LOCUS       DAOMFD010000001        40828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_95_length_40828_cov_34.968631, whole genome shotgun sequence.
ACCESSION   DAOMFD010000001 DAOMFD010000000
VERSION     DAOMFD010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 40828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40828
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95_length_40828_cov_34.968631"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="PL152_00005"
     CDS             <1..225
                     /locus_tag="PL152_00005"
                     /inference="COORDINATES: protein motif:HMM:NF027525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="band-7 C-terminal domain-containing protein"
                     /protein_id="HQR47680.1"
                     /translation="ANATADAIRKIAEAIRSPGGDQAVQLKVAEKAVEAYSQLAQKTN
                     AMIVPGNMTEVSSLIATAMAVFRAKSGGTP"
     gene            225..521
                     /locus_tag="PL152_00010"
     CDS             225..521
                     /locus_tag="PL152_00010"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HQR47681.1"
                     /translation="MKRHVAACLALAALAASTAAFGDDDTRCGSQVVTIGMSKSDVQA
                     ACGDPATSEDIPQDVRDGDMVVGQSMQSRWTYVSGAVKTTFVFDQDKLIEIHVD"
     gene            600..3503
                     /gene="cphA"
                     /locus_tag="PL152_00015"
     CDS             600..3503
                     /gene="cphA"
                     /locus_tag="PL152_00015"
                     /EC_number="6.3.2.29"
                     /EC_number="6.3.2.30"
                     /inference="COORDINATES: protein motif:HMM:NF010623.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0051235 - maintenance of location [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycin synthetase"
                     /protein_id="HQR47682.1"
                     /translation="MRILDRSVYVGPSLYAHFPVIRLELDLGELEAWPTGRLGAGFVD
                     ALAVALPGLAEHGCSYQVPGGFFRRMREDDGTWLGHVLEHVAIELQNMAGEEVTFGKT
                     RSADRPGVYTVVYEYAQRDEGIAAGELGLRLLCSLLPEGIRPADTVPEGWSWPEARDE
                     FIRYAQRRALGPSTASLVRAAESRGIPWLRMNDQSLVQLGHGKYQQRIQATVTGRTPH
                     IAVELASDKEETNKILATLGLPVPRQELVQSEGQAVRAARRIGFPVVTKPYNGNHGRG
                     ISIRLTTDEEVAHGFTVAREHSRSVIVETYLEGDDHRLLVVNGELVAATRRTPGHVVG
                     DGEHTVAQLVEIVNQDPRRGVGHEKVLTRLEIDAQAQKMLERAGLAADSVPDAGRVVY
                     LRSTANLSTGGTATDVTDVIHPDNREMAERAVRAIGLDVGGVDFLSSDITESYRRIGG
                     GICEVNAAPGFRMHVAPSEGTPRDVAGPVIDMLFPPGTPSRVPIAAITGTNGKTTTAR
                     MLAHVTKMAGYTPGLTTTDGVYIDGQRTVQGDMTGPVSARMVLADPQIDIAVLETARG
                     GLIRAGMGVNEVNVGAVLNVQSDHLGLKGIDTLEQLADVKRVVVEVATDCAVLNADDP
                     LVLRMAGYSDAKNICYVTMNPQHTLVREHIRAGGRACALEAGVNGQMITLYDRGGHIP
                     LVWTHLIPATLEGRALHNVQNAMFAAAMAFSLGIKLDAIRQGLRTFDSTFYQAPGRMN
                     VFDEHPFKVLFDYGHNAHAVGMLADLAGRLEVKGRRIVVLAGPGDRRDEDLVAIANAV
                     AGRFDHYVCRRDDSLRDRAPDEVPRIQAAALRAAGVPDAAISIIPDEQEAIDAALRMG
                     REGDLLLVFADALVRSWKQVTKFRPAAVSMPELRSPDAGTIAVVESTRQEPLRVSAAD
                     GLDDAVPGPTFTPSHAAAAGRGLAEGGPVPPAVSLEGLIRDERGVRIAPETED"
     gene            3518..5368
                     /locus_tag="PL152_00020"
     CDS             3518..5368
                     /locus_tag="PL152_00020"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQR47683.1"
                     /translation="MPFEDSRRLTGCNLFFASTGAVLELAGIAADETLLAGWRARAER
                     ARTALGWTDSRMVGSERRSDAEPHGTAARLHASGAAFAVSAPCDQLFTATEVNEWALC
                     AALYERDPLHWASLEDAMRVAAVEAAEGRPVDDPPVLDETQAIARLRRLAAGEARPDV
                     VALIAAADAHDLPHVLDETVLTVGAGAGSRSYPLDALPQIASVPWGELRDIPTAVVTG
                     SNGKTTTVRLVAACARAQGWKAGYNCTDGVVIGDETIATGDYAGPAGARMVMRDPRAE
                     AAVLETARGGILRRGIAVSRAHVAVVTNVSPDHFGEYGIHDLAGLADVKLTVASVLGR
                     DGLLVLNADDPILRSREGTLAQRFGRQPQIGWFALDADAPHLQAHRGHGGWTCGVRDE
                     RLRLSVGSIEHDLGLVSAMPLTVDGSADYNIANLGAAALASAALGVAPVAIAAVFATF
                     GAGRGDNDGRLMRFDVGGVHVLVDYAHNPDGIRGLLRVATHLRRRGGRLGLLLGHAGN
                     RQDAALEDVARVAAGFGPDLVVVKEDEAHLRGRAPGEVPRIIRDALLRHGLPPAAVPL
                     RGSEMDAVRCALDWARPGDVLALPVHGGAARKATIELLSEHHAWNGSGSG"
     gene            5344..6336
                     /locus_tag="PL152_00025"
     CDS             5344..6336
                     /locus_tag="PL152_00025"
                     /inference="COORDINATES: protein motif:HMM:NF014476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HQR47684.1"
                     /translation="MERVGIRVIGGEDVRRLLPYADCVEAVAAAMQQVSAGRTVMPLR
                     QVMRLPGGRGALGTMPGYLGEPECFGIKVLSLFPGNPAVGLSSHLGLYMLCEAVNGRP
                     VALMDASALTAIRTAAASVVATRALARSDSRVLAIVGTGEEARSHVDAFQAVRPFERL
                     LIWGRNHTSAQGLAAYARDAGVPAVRVVATVKDALEEADVVCTVTSAPDPLLFGADVR
                     PGTHLCLVGASIPSTREVDDACVAMARYFVDYRASAMAQAGELLHAMRDGVVDQAHVL
                     AEIGEVLGGSATGRRSAEDVTIYKSLGIAAQDLAAAMLVHRRAVELGVGTVVPI"
     gene            complement(6361..8121)
                     /locus_tag="PL152_00030"
     CDS             complement(6361..8121)
                     /locus_tag="PL152_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HQR47685.1"
                     /translation="MSRTTRRAACLVLSGVLGLVACQHAPTNAGRAGATAPPANAGPP
                     SATHHAGVAAANPLAVEAGLEILKRGGSAVDAAVAIQAMLGLVEPQSSGLGGGAFMVV
                     LDGRTGKVTAFEGRETAPAGARPDMFLGEDGKPLGYADAVTSGRSTGAPGAIAMLGIA
                     HERFGRLPWRELFGTATRAADEGFTVPKRLARFAGGNFPQSALPDTRALLSRPDGTPV
                     QAGDTLRNPAYAATLRIVAAQGSRAVLEGSIAQRIAARTHDDPRPGTMTTADLAAYRP
                     HQVDALCRPYRIYVVCVPPPPSSGVSLLQMLAILEHTDIDKRGPSDPQAWFLFAEASR
                     VMYADRDRYVADPAFVRVPVNGMLDPGYVASRAALIGATAGPAPTAGDPPGATARLGR
                     DATTEAAGTSHFVVVDGDGTVVSMTTTVESLFGSGRAVDGFFLNNQLTDFSFIAVDDG
                     RPVANAVAPGKRPRSSMSPAVILDRDGHFYAALGSPGGSQILTYVAKAIVGLVDWHLT
                     MQQAIDLPNLGGRGNDFYGEVSKFPPEVLAGLAARGIVVKSGRGEESGLHGVVVRGGG
                     TLEGGADTRREGEWREVVQP"
     gene            complement(8154..8981)
                     /locus_tag="PL152_00035"
     CDS             complement(8154..8981)
                     /locus_tag="PL152_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47686.1"
                     /translation="MTADAPRERDLRRVDEAEAAAFRDMCRAAPSEFVHGSGLRCLDA
                     GGATLVVAPGLPTTMFNRAIGLGVHRPAAEADLESVISEFRSAGSSNYWVHLNPGSSP
                     PQIGAWLEARGFEPARRRSWAKVVFDGARAPDTQTSLDIREVGVEHADELAAVLAAAF
                     EMPPAFASWFRALVGRPNWHAVGGFDGKALVCGGFLYRERDFGWLGIGGTRTESRGRG
                     GQRAVMALRMRIALGHGARWIATETGEPIDGESNPSLANMYRCGFRHVCSRLNYEPG"
     gene            complement(8978..10666)
                     /locus_tag="PL152_00040"
     CDS             complement(8978..10666)
                     /locus_tag="PL152_00040"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR47687.1"
                     /translation="MTAPLRATLFISLALCALGAGVSRAGPGVLVNAHIRTLDDQRPS
                     AEAMAWGDDGRIVAIGRRDEVLAHSPGLPVFDAGGATVVPGLIDAHAHLMGLGYALMS
                     VDLVGTRSRTDVVARLKAHAAALPPDAWLLGRGWDQNDWPIREFPTAADLDAAFPTRP
                     VWLERVDGHAGWANSAAMRLVRRDLGGDWQPEGGRIVRAGGKATGVFIDNATALIDGI
                     VPAPSDEVRAEALRRALAATASVGLTGVHDMGTSLEDLALYRRLADQGALTMRIVAYA
                     DGEHAAFEELCRNGPYRHAGGRLTMPGVKFYADGALGSRGAALLAPYSDDPGNRGLLV
                     TPPDRLSSDIRKARRCNLQVATHAIGDRGNRIVLEDYALALSDQARSDHRWRIEHAQV
                     VALNDVSIFAELRVIASMQPTHATSDMPWARDRIGSQRIEGAYAWQRFRQARVRLALG
                     SDFPVESPDPRLGLYAAVTRQDLAGNPPGGWYPRQRLTRLEALQGFTTDAAWAGFMEH
                     EVGHLAPGMRADFVVLDRDPLTVPPTELPTMKVLSTWLDGAKVFDSASTGPAQP"
     gene            complement(10715..11581)
                     /gene="htpX"
                     /locus_tag="PL152_00045"
     CDS             complement(10715..11581)
                     /gene="htpX"
                     /locus_tag="PL152_00045"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HQR47688.1"
                     /translation="MKRILLFVATNIAVLMVISVVLRVLGIDQAMAAQGGMGPLLAFS
                     AVVGFTGAIISLLMSKFIAKMSTGAQVITGAEGESQRWLVETVRKLADKAGIGMPEVA
                     VYDGPPNAFATGAFRNSALVAVSTGLLQSMNRDEVEAVLGHEIGHVANGDMVTLTLIQ
                     GVLNTFVVFLSRIVGSIVDKAVFNSERGTGPGYFITVIVCQILFGILASMIVAWFSRQ
                     REFRADAASAGLLGSSTPMVSALRRLGGLEPTALPEAMKAFGINDKPGFMALFSTHPP
                     IEQRIAALQQRG"
     assembly_gap    11704..11803
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11804..12336)
                     /locus_tag="PL152_00050"
     CDS             complement(<11804..12336)
                     /locus_tag="PL152_00050"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47689.1"
                     /translation="MTRSTLHEAAGELARRDTALRGIHERLGTPPLWGRQPGFATLVH
                     IILEQQVSIPAARTLFLRLKRHLGGVTAERVAATPIVALQRFGLTRQKAGYCHGLALR
                     IVDGRLDLGAAARAPDELGRRELLAVPGLGPWSVDIYYLMALRRPDVWPTGDLALAVA
                     MRELEGMSRLPTRDEQA"
     gene            12449..13351
                     /locus_tag="PL152_00055"
     CDS             12449..13351
                     /locus_tag="PL152_00055"
                     /inference="COORDINATES: protein motif:HMM:NF006128.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQR47690.1"
                     /translation="MTFRTLKYAVEGRVATITLDRPGRMNAISLEMPGEIEAAVHAAN
                     HDDAVHVIVLTGAGDGFCSGYDLVEFAEKAGEQPGSQLGSGTTETRPWDPMADYFAMY
                     DFTQQFMSLWRSYKPTIARVHGPAVAGGSDIALCCDLVVMAEEAVIGYPPARVWGCPT
                     TAMWVYRLGAERAKRMLLTGDLVDGREAARLGLVLEAVPLSRIDERVAAIAARIAAVP
                     KNQLMMHKLMVNQAYDAMGLANTQRFATLFDGITRHSPEGLWFKRRAEEAGFKQAVRE
                     RDGGEPIAPDASRPLAPRREGERS"
     gene            13348..15036
                     /locus_tag="PL152_00060"
     CDS             13348..15036
                     /locus_tag="PL152_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HQR47691.1"
                     /translation="MRIVDVGGHADALESRTLCEAFQATAAERPGQVAHRTLDGSVEF
                     TYAEFAERVRALATGLHSLGVRRGDTVALMLRNRPEFALADAAAMHVGATAFSVYNTL
                     SPEQIGHLFRNARNRVVVTELMLLPRVLAARTADVEHIVLVDGEAEGAMTLADLGRRS
                     SPGFRFEDAWREVEPGDVLTLIYTSGTTGPPKAVQLTHANVMFEARAIATAMPWDRGG
                     RGISYLPSAHVGDRTLVHYVSSMCLGRTITSIDDYRRIGEALVSVRPTIFGGVPRVWE
                     KIKAALEVAGVRAPGQLPEPVKAAVRERVGLDQAAWSVSSAAPASPEVLRYFNDLGIV
                     LCEGWGMSELSCLATMNPLDDLRPGTVGRALPGVELKLLDDGELLVRAPLVMKGYRDD
                     PEKTAEAIDPQGWLHTGDVAQIDGDGYVTIVDRKKEIIINSAGKNMSPANIEFRLRAA
                     SPLIAQAVCIGDRRPYNVALLVLDPEPAARWAADRGLPAGLDALCHRPELRAALDAAV
                     AAANEGLSRVEQVKRYAVIGSEWLPGGDELTPTSKLKRKPIGEKYSAEIERLYA"
     gene            complement(15056..16972)
                     /locus_tag="PL152_00065"
     CDS             complement(15056..16972)
                     /locus_tag="PL152_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR47692.1"
                     /translation="MSPPPPGPSAAVRVLVVDDEPAVIDAYRQVLDASPASADRAALD
                     ALRTRLFLSGGSPALLAKQPPRPKPFDATYCTGAHAAVAAVREANAANQPFAVAFLDM
                     RMPPGPDGVWTAQKIREIDPQVEIVVCTAYSDVDPLEIGRRVPPADKLFYLQKPFHPH
                     EIRQMAAALGEKRANTDRRLSELSDADDLTGLPTRAKFLARLREAVDDARGHGRSMAL
                     FYLDIDNFRRINDALGHTVGDEMLRRVAVRLREALQRDDVSNCIAGRGAGPDVARLGG
                     DQFVVLVDGLREPADAALIAQHLAGPMLASNDADQTQVTLTASVGIAIYPTDSEDDEA
                     LLRQSGIAMYAAKRQGRGNFAYFNATMKDGAQARFGLESRLQGALERGELSLHYQPQF
                     DLGTGRVSGIEALLRWTNRELGKVPPEEFIPMAEETGLIVPLGEWAMRTACEQFRRWL
                     DMDLEPGRIAVNVSPAQFAHPGFCSMVTDVLRDTGIEPRNLEIEITESLAMRDDDRTR
                     DIIAELRATGVSIAIDDFGMGHSNLSRLSTIAVNRLKIDRTLVHAVDSVGRRSTIVGA
                     IVSMARALGLQVVAEGVEDFEQLLNLQEQQCNEVQGFLLSKALPVDEATQLLQRLEAS
                     TATSRTMRLRSLAF"
     gene            complement(16969..>17212)
                     /locus_tag="PL152_00070"
     CDS             complement(16969..>17212)
                     /locus_tag="PL152_00070"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR47693.1"
                     /translation="LRFTDDGVGIADQDLTRVFENGYSTKSRATNHGIGLHWCANALQ
                     AIGGSITAESSRGKGATLRVVVPVQRSVRPATERAA"
     assembly_gap    17213..17312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17313..19010)
                     /locus_tag="PL152_00075"
     CDS             complement(<17313..19010)
                     /locus_tag="PL152_00075"
                     /inference="COORDINATES: protein motif:HMM:NF017078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE4 domain-containing protein"
                     /protein_id="HQR47694.1"
                     /translation="MSLYSKVVVLLCALFLAYGATDYVVQRAVILPSFEKLEGDLART
                     DMERVSRAIDGELSQLLTFCADWGNWLETYQYMAGRNARFIDDNMTPATIVAAGLDAV
                     AYVDDQGRFVWRQGFRPGTDQELAYRFLEGEGLGRNHPFARAIREGRPAKGLVQTEHG
                     PAMIVTAPVLDGAGNGPHHGAVVLARVITPAVAARLAEQAQVKLQVLAASAANPAGAK
                     SGPATRPRILQGPHANEVYLDLNDIYGRPAVTLRIDVPRSVTRQGRDAVRYALISIFV
                     AGIVVLLALLAALRRMVLRPVSQLTRHAVAIAEGSDLTSRVDVRRRDEIGILAREFDR
                     MVDKLAGARRSLVDQSFEAGAAQAASGVLHNVGNAMTPLSVSVASLQEKLRGAPLGEI
                     EMVLAELENRSIEPARRDDLEQFLKLAVRELVRTHAAALDDADAVARRAEAIQSVLAQ
                     QLRESRAGPVIETVQLASLLDRSVELVPPALRRLLAIEFDTSVIDVGALPLPRITLQQ
                     VFQNLIQNAAESVHEAGLERGRLRISARLEAGPDGAARLTLRFTDDGVGIADQDLTRV
                     "
     gene            19273..19728
                     /locus_tag="PL152_00080"
     CDS             19273..19728
                     /locus_tag="PL152_00080"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR47695.1"
                     /translation="MNGIAHIQLTVNDFAACRAFYAKLFEWLEMRVIFDSPQAFYGVG
                     SRTGIAICPAQDEFRGARFEQRRVGLHHLCLRARRREDIDAMHAFLQSIGARIVHPPE
                     EGPWAPGYYSVLFEDPDGIRLEANYVPGKGNLAEGRALPLDVERMARDD"
     gene            19883..20263
                     /locus_tag="PL152_00085"
     CDS             19883..20263
                     /locus_tag="PL152_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005416164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR47696.1"
                     /translation="MHLSRLCGFIIDCNTPDLQSAARFWGAALGMPLQQLPAGEGEKY
                     VRLVDPRERLHVEVQAVTHPSRVHLDIEADDIEAEVRRLEALGAQRVEKVQTWWVMQA
                     PTGQRFCVVRAQSKDFEGGANRWP"
     gene            20338..20709
                     /locus_tag="PL152_00090"
     CDS             20338..20709
                     /locus_tag="PL152_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR47697.1"
                     /translation="MAGNPVRWFEIYVQDMTRARAFYETVLGRKLEKLDSGDLEMWSF
                     PMQPGQSGIGGALVRVPGVPSGGGGTLVYFGSDDCAIEASRIAPAGGRLHRDKMSIGK
                     YGYIVLGVDTEGNMFGVHSTK"
     gene            20710..21333
                     /locus_tag="PL152_00095"
     CDS             20710..21333
                     /locus_tag="PL152_00095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47698.1"
                     /translation="MNRRRTLLAAGLVLALVARVAGAIDAGPLSITAVPPADPQPASH
                     PWAVYLDGYIDPGAAGRLAGLMQRSGIRHAVVFLNSPGGSLLAAMDLGRLLRAGGFDT
                     NVGRRAPDGREPEAGVCYSSCPFAYAGGVRRSLLRGSALGVHRPRNLTPVVDPQAFER
                     RVSADATDYLEGMGVNPVLASIMAQAPHDAIRLVGRGEAERLALVND"
     gene            21395..22279
                     /locus_tag="PL152_00100"
     CDS             21395..22279
                     /locus_tag="PL152_00100"
                     /inference="COORDINATES: protein motif:HMM:NF002043.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloalkane dehalogenase"
                     /protein_id="HQR47699.1"
                     /translation="MKILRTPDARFEQIPDYPFAPHYHQLTDQLRLHYVDEGPRGAPP
                     VLMMHGEPTWSYLYRHMIVPVAAAGLRVLAPDMIGFGKSDKPAAKSDYTYAGHVAWMR
                     HWLEALDLRDITLVCQDWGSLIGLRLAAELPGRFARVLLSNGGLPEGTGAPRAFRRWR
                     RFARYSPLFPIGRIVQAATRRRLSPAEIAAYDAPFPANAHKAGARIFPALVPVEPDDV
                     AVPDQRRAWQAFEAWTRPFICCFSDGDPITRGGDAPFLRRVPGTKGQPHVTLHGGHFI
                     QEDDPEGFVRAILSSCGR"
     gene            complement(22299..23894)
                     /gene="ahpF"
                     /locus_tag="PL152_00105"
     CDS             complement(22299..23894)
                     /gene="ahpF"
                     /locus_tag="PL152_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016668 - oxidoreductase activity, acting
                     on a sulfur group of donors, NAD(P) as acceptor [Evidence
                     IEA];
                     GO_process: GO:0006805 - xenobiotic metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009398 - FMN biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HQR47700.1"
                     /translation="MLDSNLRQQLQAHLQRISHPVELVAHLDDTEASRELGSLLTDVA
                     SLSSLIRLETRRAAGPEGQVRTPSFSVARAGSTPRIRYAGLPMGHEFTSFVLSLLQAG
                     GHPPRLPDGVAERVQALEGDFEFEVYVSLSCQNCPEVVQALNAMAALSPRIRSTMIDG
                     ALFGAEIDARRVMAVPAVYLNGQPFGHGRMTAEEILAKLDAGNPARHAAALDAKDPFD
                     VLVVGGGPAGAAAAIYAARKGIRTGLVAERFGGQVMDTMGIENFISVTATEGPRLAAA
                     LEEHVRSYEVDLMNLQRATALHPADESGLATVELASGARLRSRAVILATGARWREMNV
                     PGEREYRGHGVAYCPHCDGPLFKGKRVAVVGGGNSGVEAAIDLAGIVAHVTLIEFDGR
                     LRADEVLQRKLRSLPNVRVLVAAETREVHGADGRVTGLSYRDRGNGAHHRLDLEGVFV
                     QIGLVPNTDWLRGAVALNPRGEIETDARGGTSAPGVFAAGDATAVPYKQIIVAMGEGS
                     KAALGAFDHLMRLPVAASAPLAA"
     gene            complement(23987..24553)
                     /gene="ahpC"
                     /locus_tag="PL152_00110"
     CDS             complement(23987..24553)
                     /gene="ahpC"
                     /locus_tag="PL152_00110"
                     /EC_number="1.11.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102039 - NADH-dependent peroxiredoxin
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HQR47701.1"
                     /translation="MTSLINTVIKPFKATAFHGGEFVQVTDADLRGKWSVVFFYPADF
                     TFVCPTELGDIADLYAEFQKIGVEVYSVSTDTHFTHKAWHDASETIRRIQYPMVGDPT
                     HAISRNFGVLIEEEGLALRGTFVVNPEGVIKVAEIHDLGIGRDARELLRKVQAAQYVA
                     THDGEVCPARWTPGAKTLAPSLDLVGKI"
     gene            24770..25792
                     /locus_tag="PL152_00115"
     CDS             24770..25792
                     /locus_tag="PL152_00115"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HQR47702.1"
                     /translation="MDKPTILLTGISGFIAKHCAVELLNAGYGVRGTVRSLGKSAEVR
                     ESLGRHAPVSRLEFVQADLTADAGWDAAVRGCEHVVHVASPFPARPPKHDDELIRPAV
                     DGTLRVMRAAAEGGVRRFVQTSSMVAVMYGHPRTRTEPFTEDDWTRVESPDVTAYGRS
                     KTLAERAAREFAAREGAALHYASVNPGFVLGPALDRDIGTSAAVIQMFLKGKYPGVPR
                     LMIPCVDVRDVARMHRLALETSAPSGGRYLCAAEVLSFLEMSLAIREKLGAEARRSPT
                     RELPDWAIRLVALFDGAARQAVPDLGRPMHVDNSRTVRTLGMKFIPAREAAVAMARSL
                     LDLHLA"
     gene            25805..27277
                     /locus_tag="PL152_00120"
     CDS             25805..27277
                     /locus_tag="PL152_00120"
                     /inference="COORDINATES: protein motif:HMM:NF018012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HQR47703.1"
                     /translation="MRPTLSVIEPALVDRVIDEALGVLEKTGVLIEDESALARLRGAG
                     IAADPASGRVLFPRAAIERALATAPSSVTLHDRDGQPAAVLQGDDVHFVPASSALRVL
                     DRRTQQAREPGTPDFVEYVKIGDGLKNISYLSTAFIPKDIPQDVADVWRLYLVLAHSK
                     RPIVSGAFTAWGVPRMGQVMAMFRRDRDDLVRRPMSMFTCCPNTPLRWGEDPVANIMD
                     CAEWGIPMEIVPVLLLGMISPVTTVGALVLHTAEVLSGLAIAQAVRPGTPVVFGGAPA
                     SFHMRLMTNPMTAVEALQLYCGYAQVAKRLNLPCQGYMALSDAKFNDPQAGMETGVGA
                     FLAACAGINSVSGPGMLDFVNCFSFEKLVFDDEVVAHAKRFVRSVEVREDLPAGELIA
                     ELVRDHHLLTSEHTLAHWPDELYLPGPTLDRMNWDQWADHGSRDWRARTNEVIDELLS
                     NYEVEPLDAGLRAEIEGLISTTCREVPGVALPAFTGDAHA"
     gene            27270..27929
                     /locus_tag="PL152_00125"
     CDS             27270..27929
                     /locus_tag="PL152_00125"
                     /inference="COORDINATES: protein motif:HMM:NF014375.2"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HQR47704.1"
                     /translation="MPDYALMNQSLFEGHHDAVRRMTEEALAEGRPPMEVLEDGLIAG
                     MRVVGEDFKHNRIFVPEVLLAARAMKAGMAVLKPLLGSKQDGASALSETIVLGTVKGD
                     LHDIGKNLVGMMAEGAGFTIVDLGTDTSAERFIAAAKEHGAKIVGMSALLTTTMIYMR
                     TVVQKFKEAGMGDVKLCIGGAPTSPDYAKQIGADGYAADAASAVELFKRLIAEHAAAA
                     K"
     gene            27932..29578
                     /locus_tag="PL152_00130"
     CDS             27932..29578
                     /locus_tag="PL152_00130"
                     /inference="COORDINATES: protein motif:HMM:NF025925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HQR47705.1"
                     /translation="MPGAAAPSGGATLTIDGLTGLAPAGQTLFDWAESRGIAVPTSCH
                     KQGKCRECLVEIETGAECLSEPAEQELHLEGRFRLACRTRVVQDGQVRAHTLRRGSMR
                     IETSSDAEAIDGVALDPAVTRDGPRILLDGEPIAEAGGPLHGVAIDLGTTTIALRLHD
                     LGTGRLVATHSFENPQRFGGTNVMSRIHYDGTHRGRLLQRTLLGYLTHAIESLPVDPA
                     TIYEIVVAGNPTMRDLLFGLDVQGLGVLPYRSTTEAALADGRATTTSLAATARRLRLP
                     AHPLARVYALPLVGSHVGADAAACLLATGIAEGTGISALLDIGTNTEAFFGNADRMLA
                     ASCPAGPAFEGGGVGSGMPALDGAIERVAIADDGSIGIRTVGDAAPIGVCGSGLVDLL
                     SELRRTGRMNAQGRFLDPDEPITLDGEGRVSVSEIDVNELAQAKGANAAGMRVLADVH
                     GVDLARVDRLYLAGGFARHLDVDAACRIGLIPDLPRERIVKVGNAALAGASVALLSRR
                     RRQDIESIVRRIELVRLEAHPRFFDFFVQGCQFEPFGAAP"
     gene            29575..30345
                     /locus_tag="PL152_00135"
     CDS             29575..30345
                     /locus_tag="PL152_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47706.1"
                     /translation="MTVASILQVSGSDAAAAVSDAEFHRLLGVPRGREVPVEVAERAG
                     LARRWYEEHGRPFVATRRLALAAISHDAVTLRSGDVLAGPALADRLRDGEAHALVVAA
                     FSAGREVADEAARLWADARPDESYFLDRFAAAVVEQLVWRASAVLCADATATGEGLLQ
                     HLSPGCGRWELSAQHALMALLLGTTGHHADAWSIGPIRLLESGALHPQHSLLAALGVT
                     RRAIVATSESACRSCDLRHCRYRRAAYARDAAEPGTTE"
     gene            30342..31256
                     /locus_tag="PL152_00140"
     CDS             30342..31256
                     /locus_tag="PL152_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014615.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQR47707.1"
                     /translation="MTQPSLLDAIKTRVLLGDGAMGTQLQNAGLESGHCGEAWNVDHP
                     DRVLGIQRRYAEAGSDVLLTNTFGACRIMLDRHGEGARTADINRASVAIARQALGGRG
                     YVLGDIGPFGGLMEPYGDITRAAVESAFREQARALVEGGADGIIIETQTAFEEVEIAI
                     AAAREAGAKVVIASFAFDRMADEDDVRTMMGISPEQAAEFMARHGCDIAGLNCGTGVD
                     MRFAADITRRYRATCGLPVMAQPNAGAPVLEDMKVLYKQTPEEMGAGLAGLLAAGPRI
                     VGGCCGSTPAHIARFRQILDGGVGGRQA"
     gene            31253..31582
                     /locus_tag="PL152_00145"
     CDS             31253..31582
                     /locus_tag="PL152_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47708.1"
                     /translation="MKVTIPVDEAAVDAYLPSAKPDGSGVGVNYADQVLKLLKVTLED
                     GRKLAARRRGLKVAVTIGEQSGHAIMRRIDDGPDTRVIFERALAAALREAGATFSREP
                     GAIHIEA"
     gene            31748..32410
                     /locus_tag="PL152_00150"
     CDS             31748..32410
                     /locus_tag="PL152_00150"
                     /inference="COORDINATES: protein motif:HMM:NF037410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47709.1"
                     /translation="MNGTRLASYKLSQDAGFAPNPFFGTLTLATCMPDMRLAAALDDW
                     IAGFTSEALCGDAVGAERLVFLMRVEEALSIAEYYSDARFARKIPVPNGAPEVGRHGD
                     NIYQPRRADAGEPEHFAQLPNPHHWDHTHRREDERSKRLDLRGRRVLLATEFVYFGRE
                     ALVVPEFARPALPRGHAPAGSSTADPELARAFIDYALGRASRPVVAPPHTWPAGDDSW
                     RH"
     gene            32460..32621
                     /locus_tag="PL152_00155"
     CDS             32460..32621
                     /locus_tag="PL152_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47710.1"
                     /translation="MRSILGWLAAFIGGASGWWLGRHVGLGTAVVLSSLGTGAGMYFG
                     YRWFDENLR"
     gene            32736..>33111
                     /locus_tag="PL152_00160"
     CDS             32736..>33111
                     /locus_tag="PL152_00160"
                     /inference="COORDINATES: protein motif:HMM:NF012360.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47711.1"
                     /translation="MSIHPTAIIEDGAQLGAGCVVHAHAIVSRHCVLGEGVVVHPFAV
                     VGGDPQDLRFDVATRTGVRIGARTVIREHVTIHRATKADGGTDIGADCYLMVGSHVAH
                     DCRIADRVIMANAVLLAGHVQIG"
     assembly_gap    33112..33211
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <33212..33646
                     /locus_tag="PL152_00165"
     CDS             <33212..33646
                     /locus_tag="PL152_00165"
                     /inference="COORDINATES: protein motif:HMM:NF025097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47712.1"
                     /translation="DRVIMANAVLLAGHVQIGEGAFIGGGATVHQFTRIGEGAMIGGA
                     ARVSRDVPPFSMTTERDEVVGLNVVGLRRRGLPKATLEELKRAFREVNVPVGNMREIA
                     AAALAGGSYATPEARRYLEFFAAGRRSFVRARRAGAALDSDE"
     gene            33682..35043
                     /locus_tag="PL152_00170"
     CDS             33682..35043
                     /locus_tag="PL152_00170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47713.1"
                     /translation="MAIEGLDAAVAAALIGIDTVWGGDVMNPSGTGRFIADSWFSGEP
                     LPSAYTRPEAAVLRSSGGVGARMPDVPAVEAYLKAIELPGTIARVIAGGRSLGGLRGA
                     HIEGLGLCCEVMLDLALEILGRGPAVPYERCVRTLTGSEPEPSRPDDKVRRVAELLGN
                     GTGSPPRGAALVAAVDEWRAARRVPRGSIASLGDRCIAEYDALTKRNLLPHLPRDMAD
                     VPRANVRFLPIENAWFSGSMNYIGRARKPDGTPEYEATYELNAALEISVPEFAQLVSH
                     EVVPGHVTTFAYLQNLYVRGMAGFEVTVQAMNTRDSVLAEGIANNAILIAHGVTELED
                     LPDRELELGVLLALLQDDAKNQASYLTWNDKVAQSEVAAVLRRDFLVSEERADKLSGA
                     WGRHPLLGRMYLPAYRAGTELVARLRRRYPPGVILPALYGCRGYADSHTLEGLLEGRY
                     GPG"
     gene            complement(35054..36439)
                     /locus_tag="PL152_00175"
     CDS             complement(35054..36439)
                     /locus_tag="PL152_00175"
                     /inference="COORDINATES: protein motif:HMM:NF016150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 domain-containing protein"
                     /protein_id="HQR47714.1"
                     /translation="MGLNAAPTGPSERIASIDAVRGIALLGILVANVRQMFLPWDVAA
                     FPVPGAHGELLGQLDWALFDAVVDLKFITLFSLLFGMGFALQSGRSAVARPGFPAIYL
                     RRIAMLAVFGALHALLLYPADVLLPYAIAGLCLYAARRLSTRNLYRIGIVLVATTVVW
                     GFQFGSLGRVSPGVTGIAMVAFTVVVALLWDRNWRAALLVVALVMAIAIAVLLLCGDP
                     RGWGRSVASEYSLALGDLAAMRSADAGAWPAEYAARRDGGFRALLQLHAQQHWLVLVW
                     FGVVLLWRTLGLNMIGAALVRSGVIAGATPRTWRRVSIVGLGIGLPMSVLATFLQSRE
                     MHGVTDWRWPEWLHVASAFPLAIGFGARVMLNEHLRKRRWWYAPMESAGRMALTNYVG
                     QSLIMAALAEPWGFGLYGSLSGPELTVLACVVFAVLALASHAWLLRYRMGPLEWVWRC
                     GTYWTWLPLRR"
     gene            complement(36430..37299)
                     /locus_tag="PL152_00180"
     CDS             complement(36430..37299)
                     /locus_tag="PL152_00180"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR47715.1"
                     /translation="MTSNSDKAILVSGASSGIGKASAGALLGAGFRVFACVRSDSAAE
                     TLERDLAADANGRLRTLRLDVTRPDQIRSAVTTVGAAVGERGLWGLFNNAGIVVAGPM
                     ECTPVDELRRQFEVNVFAQVAMTQAFLPLLRRARGRILNTGSIAGFGTVPGLGPYSMS
                     KHAMEALSDALRRELRPWGIEVSLLEPGSIATEIWSKGADAFEANLKAPPPGLVELYG
                     PLTRTLNQFAAQSARDASPVSVVTKDVVHAFTAARPRTRYCKGHGSGVRKVLRRLPDR
                     WVDAVFARTLRWG"
     gene            37411..39114
                     /locus_tag="PL152_00185"
     CDS             37411..39114
                     /locus_tag="PL152_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47716.1"
                     /translation="MRFVSPCTVATLAAVGFAMQANAGDVVAAKPVAAAEAAFLDFLD
                     AHGELGVVESGLQERYLGRDRAQWQALSDSRRNRLDAALGRLKPLQLRPADERAVQAM
                     LASLAALASEDAVSNSPATSCKDAGERDLDYARLRAALVACYVEYGNALRFEDSTIDR
                     GSALQLLHVIDEPARRRAVFDAFVPLWTALDGHGEPDSPYRRMIAMAAEAARKQGSPI
                     DAAARALGVRETDVENWLVEVLDAWRQATGPQLVEPWDYRYVNSAANRELAARIPPES
                     LVPANQRFYQDLGADLAALGVMYDLEPRAGKSPLAYTDFVTRGRTTGGRWQVSVARVV
                     ATYPTGGLYSLNELVHENGHAVHIAAIHNRPAYMDWPDTLFTEAFADVPSWSVYEPAW
                     QRRYLGAAAAEPASLRALFGDVMLDVAWALFELRLLRDPRQDPNVLWTDITSRYLHVA
                     PHPEVPWWAVRVQLVDEPGYMVNYGLGAVLTAEMRQRTVDAVGVFDAGNPGWYAYLSR
                     QLLQYGSERGTRSLLQDLLGRPVSPAALIRQVARSGDAAASGSTVTGNQGTFPYFASS
                     K"
     gene            complement(39154..>40828)
                     /locus_tag="PL152_00190"
     CDS             complement(39154..>40828)
                     /locus_tag="PL152_00190"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HQR47717.1"
                     /translation="AMPSSRVQRTLPPPLPEWFPRGTALLRDPTLNKGTAFTEHERDV
                     LGLRGLLPPHVCTQEDQVARVLENFHRLQTPLAKYILIEALQDRNEALFFRVIVENPD
                     EMMPIIYTPTVGLACQQFGHIYRRPRGLFISAADRGRIAQVMRNWPHREAAMIVVTDG
                     ERILGLGDLGANGMGIPIGKLALYTACAGLHPRRCLPVMLDVGTNNEALLRDPLYLGL
                     RQRRLSGGALDELVEEFIEATQQVFPGIVVQFEDFANHNAFRLLERYRDRVCCFNDDI
                     QGTASVTVAGIFSALRVTRRRMSEQTFLCLGAGEAATGISDLLVAAMTAEGMGIAEAR
                     RRCWMVDSKGLVVAGRQGLAAHKIPYAHEHAPVPDFLGAVKALQPTAIIGVGATPGLF
                     TKDVIEEMSRANERPIVFALSNPTSKSECTAEQAYAWSGGRALFASGSPFDPVTLDGR
                     RYVPRQGNNSYIFPGVGLGVVAVRATRVTDEMFMAAARELARHVNESDLAQGSLYPPL
                     NRVRDVSAHIAAAVADVVFSRGLAGMERPDDLYEYMRSQMYEPRYAAYA"
BASE COUNT         6093 a        13892 c        14499 g         6044 t
ORIGIN      
        1 gcgaacgcga cggccgacgc aatccgcaag atcgccgagg cgatccgctc tcccggtggc
       61 gaccaggccg tccagttgaa agtggcggag aaggccgtcg aggcctacag ccagctggca
      121 cagaagacca atgccatgat cgtgccgggc aacatgaccg aggtctcctc gctcatcgcg
      181 acggcgatgg cggtgttcag ggcgaagtcc ggaggtactc cttgatgaag cggcatgttg
      241 cggcgtgcct ggcgctggcg gccctggccg catcgaccgc ggccttcggc gacgacgata
      301 cgcgctgcgg cagccaggtc gtcacgatcg gcatgtcgaa gtccgacgtg caggcggctt
      361 gcggggatcc ggcgacgagc gaggacatcc cgcaggacgt gcgcgacggt gacatggtcg
      421 tcggccagtc catgcagtcc cgatggacct acgtctccgg tgccgtcaag accacgttcg
      481 tcttcgacca ggacaagctg atcgagatac acgtcgacta ggcgccggcc cttttcccag
      541 ggcccccgcg ctgtgccatg atccacgacc cggcccgtca gggggaatgg caggcagtca
      601 tgcgcatcct ggatcgttcg gtctacgtcg ggccgtcgct gtacgcccac tttcccgtca
      661 tccgcctcga actcgacctg ggcgagctcg aggcctggcc gacgggacgc ctcggtgccg
      721 ggttcgtgga cgcgctcgcg gtcgcgttgc ccgggctcgc ggagcacggc tgctcgtacc
      781 aggtgccggg cgggttcttc cgccgcatgc gcgaggacga cgggacctgg ctcggccacg
      841 tgctcgagca cgtcgccatc gagctgcaga acatggcggg ggaggaggtg acgttcggca
      901 agacccggag cgcggaccgc ccgggcgtgt acaccgtcgt ctacgaatac gcgcagcggg
      961 acgaaggcat cgccgcgggc gaactcggac tgcggctgct ctgctcgctg ctgccggaag
     1021 ggatccgccc cgccgacacg gtcccagaag gctggagctg gcccgaggcg cgcgacgagt
     1081 tcatccgcta cgcgcagcgc cgggcgctgg gtccgtcgac ggcatcgctg gtccgggcgg
     1141 cggaatcgcg cggcattccc tggctgcgga tgaacgacca gtccctggtg cagctcgggc
     1201 acggcaagta ccagcagcgc atccaggcga cggtcaccgg acgcacgccg cacatcgcgg
     1261 tcgagctggc gagcgacaag gaggagacca acaagatcct cgcgacgctc ggcctgccgg
     1321 tgccgcggca ggaactcgtg cagagcgagg gccaggccgt gcgcgccgcg cgccgcatcg
     1381 ggtttccggt ggtcaccaag ccgtacaacg gcaatcacgg ccgcggcatc tcgatccggc
     1441 tcacgaccga cgaggaagtc gcgcacggtt tcacggtcgc gcgcgagcac tcccgctcgg
     1501 tgatcgtcga gacctacctc gagggggacg atcatcgatt gctggtcgtg aacggcgagc
     1561 tcgtggcggc gacgcgacgc acgccggggc acgtggtggg tgacggagag cacacggtcg
     1621 cgcaactcgt ggagatcgtg aaccaggacc cgcgtcgcgg tgtcgggcac gagaaggtcc
     1681 tcacgcgcct cgagatcgac gcccaggccc agaagatgct cgagcgggca gggttggccg
     1741 ccgactcggt tcccgacgcg ggacgggtcg tgtacctgcg ctcgaccgcg aatctctcga
     1801 ccggcggcac ggccaccgac gtcaccgacg tcatccaccc cgacaatcgc gagatggcgg
     1861 agcgcgcggt gcgcgccatc gggctggatg tcggtggggt ggacttcctg tcatccgaca
     1921 tcaccgagag ctaccgcagg atcggcggcg gcatctgcga ggtgaacgcg gcgcccggct
     1981 tccgcatgca cgtcgcgccc agcgaaggca cgcctcgcga cgtcgccggc ccggtcatcg
     2041 acatgctgtt tccgcccgga accccctcgc gcgtgccgat cgcggcgatc acgggcacga
     2101 atggcaagac caccaccgcg cgcatgctcg cgcacgtcac gaagatggcg gggtatacgc
     2161 ccggtctcac cacgaccgac ggcgtgtaca tcgacggcca gcgcaccgtc cagggcgaca
     2221 tgaccgggcc cgtctctgcg cgcatggtgc ttgccgatcc gcagatcgac atcgcggtgc
     2281 tggagaccgc gcgcggcggc ctcatacgcg cgggcatggg cgtgaacgag gtcaacgtcg
     2341 gcgccgtgct caacgtccag tcggaccacc tcggcctcaa gggcatcgac acgctcgagc
     2401 agctggcaga cgtcaagcgc gtcgtggtcg aggtcgccac cgactgcgca gtcctcaacg
     2461 cggacgaccc gctggtgctg cgcatggcgg gctacagcga cgcgaagaac atctgctacg
     2521 tgacgatgaa tccgcagcac acgctggtgc gcgagcacat ccgcgccggc ggccgcgcct
     2581 gtgcgctcga ggccggcgtc aacgggcaga tgatcacgct ctacgaccgt ggcggccaca
     2641 tcccgctcgt gtggacgcac ctgatcccgg ccacgctcga ggggcgggcg ctgcacaacg
     2701 tgcagaacgc gatgttcgcg gccgccatgg cgttctcgct cggcatcaag ctcgatgcca
     2761 tccgccaggg gctgcgcacg ttcgactcga cgttctacca ggcgccagga cgcatgaacg
     2821 tgttcgacga gcatcccttc aaggtgctgt tcgactacgg gcacaacgcg cacgcggtcg
     2881 gcatgctcgc ggacctcgcc gggcggctcg aggtcaaggg ccggcgcatc gtcgtgctcg
     2941 cgggccctgg cgaccggcgc gacgaggacc tcgtcgccat cgcgaacgcc gtcgccggcc
     3001 gcttcgatca ctacgtctgt cgccgcgacg acagcctgcg cgaccgtgcg ccggacgagg
     3061 tgccgcgcat ccaggcggct gcgttgcgcg cggcgggcgt tcccgacgca gcgatctcga
     3121 tcattccgga cgagcaggag gccatcgacg cggcattgcg catgggtcgc gagggcgacc
     3181 tgctgctggt cttcgccgac gcgctcgtgc gttcctggaa gcaggtcacg aagttcaggc
     3241 cagcggcggt ttcaatgcct gaacttcggt cacccgacgc gggcaccatt gccgtcgtcg
     3301 aatccaccag gcaggagccg ctgcgagtgt cggctgcgga cgggctggac gatgcggtgc
     3361 cgggacccac attcacaccc agccacgcag ccgccgctgg acgcggactg gccgaaggcg
     3421 gcccggtgcc gccggccgtc agcctcgaag ggctgattcg cgacgagcga ggcgtgcgca
     3481 tcgcaccgga gaccgaagac tgacggtcca gctcgcattg ccgttcgagg actcgaggcg
     3541 cctcaccggc tgcaacctgt tcttcgcctc gacgggcgcg gtactcgaac tggcggggat
     3601 cgccgccgac gagacgctgc tggcgggatg gcgcgcgcgt gcggaacgcg cgcggaccgc
     3661 gctcgggtgg actgactcga ggatggtcgg tagcgagcgg cggtcggacg cggagccgca
     3721 cgggacggcg gcacgattgc acgcgtctgg tgcggccttt gccgtgtcgg ccccatgcga
     3781 ccagttgttc accgccacgg aagtcaacga atgggcgctg tgcgccgccc tgtacgagcg
     3841 cgatcccctg cactgggcca gtctcgagga cgcgatgcgt gtcgcggcgg tcgaggcggc
     3901 cgagggacgg ccggtggacg atcctccggt cctcgacgaa acccaggcga ttgcgcggtt
     3961 gcggcgactg gcggccggcg aggctcggcc cgatgtcgtc gcgctcattg cggcggccga
     4021 cgcgcacgac ttgccgcacg tcctggacga gaccgtgctg acggtgggcg cgggcgccgg
     4081 cagtcgcagc taccccctcg acgcattgcc gcagatcgcc tcggtcccgt ggggcgaact
     4141 cagggacatc ccgaccgcgg tcgtcaccgg ctcgaacggc aagacgacga ccgtgcgcct
     4201 ggtcgcggcc tgcgcacgag cgcagggctg gaaagccggc tacaactgca cggatggcgt
     4261 cgtgatcggt gacgagacga ttgcgaccgg cgactacgcg ggtccggcgg gcgcgcggat
     4321 ggtcatgcgc gatccgcgag cggaggctgc cgtgctcgag accgcgcgtg gcggcatcct
     4381 gcgtcgcggc atcgcggtca gccgggctca cgtggccgtg gtcaccaacg tgagcccgga
     4441 tcatttcggc gagtacggca tccacgatct cgctggactg gcggacgtga agctcaccgt
     4501 cgcgtcggtc ctcgggcgcg acggactgct ggtgctcaac gcggacgacc cgatcctcag
     4561 gtcgcgggag ggcacgctcg cgcagcggtt cgggcgacag ccgcaaatcg gctggttcgc
     4621 gctcgatgcg gacgcgccgc acctccaggc tcaccgcgga cacggcggtt ggacctgcgg
     4681 cgtccgcgat gagcggctga gattgtcggt cggatcgatt gaacatgacc tgggtctggt
     4741 ctcggccatg ccgctgaccg tcgacggcag cgcggactac aacatcgcga atctcggggc
     4801 cgccgccctc gcgtccgccg cgctgggcgt ggcgcccgtc gccattgcgg ccgtgttcgc
     4861 gaccttcggt gcaggccgcg gcgataacga cggccgcctg atgcgattcg acgtcggggg
     4921 cgtgcatgtg ctggtcgact acgcgcacaa cccggacggg attcgcggac tgcttcgggt
     4981 cgcaacgcac ttgcgccgtc gaggcgggcg cctgggcctg ctgcttggcc acgcgggcaa
     5041 ccggcaggac gcggccctcg aggacgtggc acgggttgct gcaggattcg gccccgatct
     5101 cgtcgtcgtc aaggaggatg aagcgcatct ccgcgggcgc gcgcccggcg aggtgccgcg
     5161 catcatccgc gacgcgttgc tgaggcacgg tcttccgccg gccgcagtgc ccttgcgtgg
     5221 gagcgagatg gatgcggtgc gctgcgcgct cgactgggcg cgtccgggcg atgtgctggc
     5281 cttgccggtc cacggcggcg ctgcgcgcaa ggcgacgatc gagctgctgt cggagcatca
     5341 cgcgtggaac gggtcgggat cagggtaatc ggcggggagg acgtgcggcg attgctgccg
     5401 tacgccgact gcgtcgaggc ggtcgccgcc gcgatgcaac aggtcagtgc cggcaggacg
     5461 gtcatgccgc tgcggcaggt catgcggctg cccggcggcc gcggtgcgct cggcacgatg
     5521 ccgggctacc tcggggaacc ggagtgtttc ggcatcaagg tcctgagcct gtttccgggc
     5581 aatcccgcgg tcggtctgtc gtcgcacctc gggctctaca tgctctgcga ggcggtcaac
     5641 gggcgaccgg tggcgctcat ggatgcgtcc gcactgacgg ccatccgcac ggccgcggcc
     5701 agcgtcgtcg cgacgcgcgc actcgcgcgc agcgactcgc gggtcctcgc catcgtgggc
     5761 accggcgagg aggcgcgctc gcacgtcgac gcgttccagg ccgtgcggcc gttcgagcga
     5821 ctgttgatct ggggccggaa tcacacctcg gcgcaaggtc ttgcggctta cgcgcgcgac
     5881 gccggcgtgc ccgccgtgcg cgtcgtcgcc acggtgaagg atgcgctcga ggaggcggac
     5941 gtggtctgca ccgtcacctc ggcgcccgat cctttgctgt tcggcgccga cgtgcggccg
     6001 ggtacgcacc tgtgcctggt cggcgcgtcg atcccgtcga cccgggaggt cgacgacgcc
     6061 tgtgtcgcga tggcgcggta cttcgtggac taccgggcga gcgcgatggc gcaggccggg
     6121 gagttgctgc acgccatgcg ggacggtgtc gtcgaccagg cgcacgtcct ggccgagatc
     6181 ggggaagtgc tgggcgggtc cgcgacgggc cgacggtcgg ccgaggacgt cacgatctac
     6241 aagtcgctgg gcatcgcggc gcaggacctc gcggcggcga tgctcgtcca tcgccgcgcc
     6301 gtcgagctcg gcgtcggcac cgtcgtgccg atctgagtgt cggtgcccac cggtcggcga
     6361 tcacggctgc acgacttcgc gccactcgcc ctcgcgtcgc gtgtcagcgc caccctcgag
     6421 cgtgccgccg ccgcgaacga cgaccccatg cagacctgac tcctcgccgc gcccgctctt
     6481 caccacgatc ccacgcgccg ccaggcccgc gagaacctcg ggcgggaact tcgacacctc
     6541 gccgtagaag tcgttgccgc gcccgcccag gttcggcagg tcgatcgcct gctgcatggt
     6601 gaggtgccag tcgacgaggc cgacgatcgc cttcgcgacg taagtcagga tctgcgagcc
     6661 gcccggcgag ccgagcgccg cgtagaagtg gccgtcgcgg tcgaggatga cggcgggcga
     6721 catggacgag cgcgggcgct tgccgggcgc gacggcgttg gcgacgggcc tgccgtcgtc
     6781 gacggcgatg aacgagaaat ccgtgagctg gttgttgagg aagaatccgt ccaccgcgcg
     6841 ccccgagccg aacagcgact cgaccgtcgt cgtcatggag acgacggtgc cgtccccgtc
     6901 gacgacgacg aagtggctgg tcccggccgc ttcggtcgtc gcgtcacgac cgagtcgcgc
     6961 ggtcgcgcct ggcgggtcgc cggccgtcgg cgccgggcct gccgtcgcac cgatcagcgc
     7021 ggcacggctt gcgacgtagc cggggtcgag catgccgttc accggcacgc gcacgaaggc
     7081 cgggtccgcg acgtagcggt cgcgatcggc gtacatcacg cgactcgcct cggcgaacag
     7141 gaaccacgcc tgcgggtccg acggcccgcg cttgtcgatg tcggtgtgct cgaggatcgc
     7201 cagcatctgc agcagcgaca cgccgctcga cggcggcggc ggcacgcaga cgacgtagat
     7261 tcgatacgga cggcagagcg catcgacctg gtgcggccgg taggccgcaa ggtcggcggt
     7321 cgtcatcgtg cccggccgcg ggtcgtcgtg agtgcgggcc gcgatccgct gcgcgatcga
     7381 gccctccagc accgcgcgtg atccctgcgc ggcgacaatg cgcagggtcg ccgcataggc
     7441 aggattgcgc agcgtgtcgc cggcctgcac cggcgtaccg tcgggtcgcg acagcagcgc
     7501 ccgggtatcc gggagcgcgg actgcgggaa gttgccgccc gcgaatcgag ccaggcgctt
     7561 cgggaccgtg aagccctcgt cggctgcgcg cgtggcagtg ccgaagagct cgcgccaggg
     7621 cagcctgcca aagcgctcgt gtgcaatccc gagcattgcg atcgccccgg gcgcgcccgt
     7681 cgagcgaccg ctcgtgaccg cgtcggcgta accgagcggc ttgccgtcct cgcccaggaa
     7741 catgtctggc cgcgctcccg caggtgcggt ctcgcggccc tcgaacgccg tgaccttgcc
     7801 ggtccgccca tcgagcacga ccatgaacgc gccaccgccg agtcccgaac tctgcggctc
     7861 gacgaggccg agcattgcct ggatcgcgac ggccgcgtcg actgcgctgc cgccgcgctt
     7921 caggatctcg agccccgcct cgacggccag cggattcgcg gccgcgacac ccgcgtggtg
     7981 cgtcgccgac ggtggacccg cgtttgccgg gggcgccgtg gcgccggcgc ggcccgcatt
     8041 ggtgggcgcg tgctggcacg cgacgagccc gaggacgccg gacaatacga gacaggcggc
     8101 gcgacgcgtg gtgcggctca tcgtggaaac cagggcaggg ccgggcggct agcttagccc
     8161 ggttcgtaat tgagccgcga gcacacgtgg cggaatccgc agcggtacat gttcgcgagc
     8221 gacgggttcg actcgccgtc gatcggctcc ccggtctccg tcgcgatcca gcgcgcgccg
     8281 tgcccgagcg cgatccgcat ccgcagggcc atcaccgcgc gctgtccgcc gcggcctcgg
     8341 gattcggtgc gggtcccgcc gatgccgagc cagccgaagt cacgctcgcg gtagaggaat
     8401 cctccgcaaa cgagcgcctt gccgtcgaac ccgcccacgg cgtgccagtt cgggcgcccg
     8461 accagtgcgc ggaaccagga ggcgaatgcc ggtggcatct cgaacgcggc cgcgagcacg
     8521 gccgcgagtt cgtcggcgtg ctcgacaccg acttcgcgaa tgtcgagcga ggtctgggtg
     8581 tcgggcgccc gggctccgtc gaacacgacc ttggcccagg accggcgtcg cgcgggctcg
     8641 aacccgcggg cctcgagcca ggcgccgatc tgcggtggcg acgagcctgg attgaggtgc
     8701 acccagtagt tgctgctgcc ggcgctgcgg aattccgaga tcactgactc aaggtctgct
     8761 tccgctgctg gtcgatggac gccgagcccg atcgcgcgat tgaacatcgt ggtcggcagg
     8821 ccaggagcca ccaccagggt ggcaccgccc gcgtcgaggc agcgcaggcc gctgccgtgg
     8881 acgaattccg acggagccgc gcggcacatg tcgcggaatg cggcggcttc ggcctcgtcg
     8941 acgcgccgca gatcccgctc gcgcggtgcg tcggcggtca cggctgtgcc ggcccggtgc
     9001 tcgcggagtc gaaaaccttc gcgccgtcga gccaggtcga cagcaccttc atcgtcggca
     9061 actccgtcgg cggaaccgtc agcgggtccc gatcgagcac gacgaagtcg gcgcgcatgc
     9121 ccggagcgag gtgaccgacc tcgtgctcca tgaacccggc ccacgcggcg tccgtcgtga
     9181 atccctgcag ggcctcgagc cgggtgaggc gctgtctcgg gtaccaccca ccgggcgggt
     9241 ttcccgcgag atcctgccgg gtcacggcgg cgtacaaccc gaggcgcggg tccggagatt
     9301 cgacggggaa atcggagccc agcgccagcc gcacgcgcgc ctgccggaat ctctgccacg
     9361 cgtacgcgcc ttcgatacgc tgggagccga tgcgatctct ggcccacggc atgtccgacg
     9421 tcgcgtgcgt cggctgcatg gaggcgatca cgcgcagctc ggcaaagatg gagacgtcgt
     9481 tcagtgcgac cacctgcgcg tgctcgatcc gccagcggtg gtcgctgcgc gcctggtccg
     9541 acagggcaag cgcgtaatcc tcgagcacga tgcggttgcc tcgatcgccg atcgcgtggg
     9601 tggccacctg caggttgcag cggcgagctt tccggatgtc gctcgacaac cggtcgggag
     9661 gagtcacgag caggcctcga ttgcccgggt cgtcgctgta gggggccagc agcgccgcgc
     9721 cacgcgatcc cagtgcgccg tccgcgtaga acttcacgcc cggcatcgtc agccgcccgc
     9781 cggcatggcg gtatgggccg ttgcggcaca gttcctcgaa cgctgcgtgc tcgccgtcgg
     9841 cgtaggcgac gatgcgcatc gtgagcgcgc cctggtcggc aaggcgccgg tacagcgcga
     9901 ggtcctcgag cgacgtgccc atgtcgtgca cgcccgtgag accgacgctt gccgtcgcgg
     9961 ccagggcgcg tcgcagcgcc tcggcccgga cctcgtcgct tggtgccggc acgatgccgt
    10021 cgatgagcgc cgttgcgttg tcgatgaaca cgccggtcgc tttgccaccg gcgcggacga
    10081 tgcggccgcc ctccggctgc cagtcgccgc cgaggtcgcg gcgcacgagc cgcatggccg
    10141 cgctgttggc ccagcccgcg tggccgtcca cgcgttcgag ccacacgggc cgtgtcggaa
    10201 acgcagcgtc gaggtcggcg gccgtcggaa attcgcgaat gggccagtcg ttctggtccc
    10261 acccgcggcc gagcagccac gcatccggcg gtaaggccgc tgcgtgcgcc ttgaggcgcg
    10321 cgacgacatc ggtccggctg cgcgtgccga cgaggtccac gctcatgagc gcgtaaccca
    10381 ggcccatcag gtgcgcgtgc gcgtcgatga gtcccgggac gaccgtcgcg ccgccggcat
    10441 cgaataccgg gagtcccggc gagtgcgcga gcacctcgtc acgccgaccg atggcgacga
    10501 tgcgaccatc gtcgccccag gccatcgctt cggcagaggg gcgctggtcg tcgagcgtgc
    10561 ggatgtgcgc attgacgagc acgcccgggc cggcccggga cacgcccgcg ccgagcgcgc
    10621 agagcgcgag cgagatgaac agcgttgccc ggagcggtgc agtcacggcc gggcgattgt
    10681 atgccgtcgc gccccggacc gccgcccgca aacgtcagcc ccgctgttga agcgccgcga
    10741 tgcgctgctc gatcggcggg tgcgtggaga acagggccat gaacccgggc ttgtcgttga
    10801 tgccgaaggc cttcatggct tcgggcagcg cagtcggctc gagaccgccg aggcgccgga
    10861 gcgcgctcac catcggtgtg gagctcccga gcagtcccgc agacgcggcg tcggcgcgga
    10921 actcgcgctg cctggagaac cacgcgacga tcatcgacgc gaggatcccg aacaggatct
    10981 ggcagacgat cacggtgatg aagtatcccg ggccggtgcc gcgctcggag ttgaacacgg
    11041 ctttgtccac gatcgacccg acgatgcggg acaggaacac cacgaaggtg ttcaggacgc
    11101 cctggatcag ggtgagcgtc accatgtcac cgttggcgac gtggccgatc tcgtggccca
    11161 gcaccgcttc cacttcgtcg cgattcatgg attgcagcaa cccggtcgaa acggcgacca
    11221 gcgcggaatt gcggaatgcg ccggtggcga acgcgtttgg cggcccgtca tagaccgcga
    11281 cctcgggcat gccgatgccc gccttgtccg cgagcttgcg caccgtctcc acgagccagc
    11341 gctgggactc gccctcggca ccggtgatga cctgcgcgcc ggtcgacatc ttcgcgatga
    11401 acttcgacat gagcagcgag atgatcgcgc ccgtgaagcc gacgaccgcg gagaacgcca
    11461 gcagcgggcc catgccgccc tgggctgcca tcgcctggtc gatgcccagc accctgagca
    11521 cgaccgaaat gaccatcagg acggcgatgt tcgtggcgac gaacagcagg atgcgcttca
    11581 tgaaatctct ccttgcccag gtgactcgat gcgggccggt gaaattttct cacgaaaacc
    11641 accggccatg ccgccgcagg attgggtcgc gcccgggccg cttcaaggtt tgacagccgt
    11701 cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntgcgcct gctcgtcccg
    11821 ggtcggcagg cgactcatgc cctcgagttc gcgcattgcc acggccagcg cgaggtcccc
    11881 ggtcggccag acgtccggcc ggcgcagcgc catgaggtaa tagatgtcga cgctccacgg
    11941 gccgagaccg ggcactgcca gcagttcgcg gcggccaagt tcgtcgggcg cccgggcagc
    12001 cgcgccgagg tccagccggc cgtccacgat gcgcaatgcg agtccatggc agtagcccgc
    12061 cttctggcgc gtgagcccga agcgttgcag cgcgacgatc ggcgtcgccg cgacccgctc
    12121 ggcggtcacc ccgccgaggt ggcgcttgag gcgcaggaaa agcgttcgcg ccgccgggat
    12181 cgagacctgc tgctcgagga tgatgtgcac cagcgttgcg aagcccggct gccgtcccca
    12241 gagcggcggg gtgcccagcc gctcgtggat gccgcgcaag gccgtgtccc gtcgcgccag
    12301 ctcgccggcc gcctcgtgca gcgtcgatct cgtcattgcg cgtggcggag gtgtcatgct
    12361 cgcggggagg agggcctgcg ggattgccga gtgtagcgcg cgctctgtta taagcatgcc
    12421 gtcacgatcc ggcgcaatcg ggggagtcat gacgtttcgg acgctgaagt acgccgtcga
    12481 gggccgcgtc gcaaccatca cgctggaccg cccggggcgc atgaatgcga tcagcctgga
    12541 gatgcccggg gaaatcgagg ccgccgtgca cgccgccaat cacgatgacg ccgtgcacgt
    12601 catcgtcctc accggcgccg gcgacgggtt ctgcagcggt tacgacctcg tcgagttcgc
    12661 ggagaaggcg ggcgagcagc cgggctcgca gctcggatcc ggtaccacgg agacgcgtcc
    12721 ctgggacccg atggcggact acttcgccat gtacgacttc acccagcagt tcatgtcgct
    12781 gtggcgcagc tacaagccga cgatcgcgcg ggtgcacggg ccggccgtgg ccggaggcag
    12841 cgacatcgcg ttgtgctgcg acctggtggt catggccgag gaggccgtga tcggctatcc
    12901 gccggcgcgc gtctggggtt gcccgacgac cgcgatgtgg gtgtaccggc tcggggcgga
    12961 gcgcgcgaag cgcatgctgc tcaccggcga cctcgtcgac ggtcgagagg cggcgcggct
    13021 cggcctggtg ctcgaagcgg ttccgttgag ccggatcgac gagcgcgtgg cggcgatcgc
    13081 ggcccgcatc gccgccgtac ccaagaacca gctgatgatg cacaagctga tggtgaacca
    13141 ggcctacgac gccatgggcc tcgcgaacac gcagcgcttc gccacgctct tcgacggcat
    13201 cacgcgccac agccccgaag ggctctggtt caagcgccgt gcggaggagg ccggattcaa
    13261 gcaggccgtg cgggaacgcg acggcggcga gccgatcgcg ccggacgcga gccggccact
    13321 ggcgccgcgg cgggaagggg agcggtcatg aggatcgtcg acgtcggcgg gcacgccgac
    13381 gcacttgagt cccgcacgct gtgcgaggcg ttccaggcga cggccgccga gcggccgggc
    13441 caggtcgcgc accgcacgct cgacggcagc gtcgaattca cgtacgcgga gttcgccgaa
    13501 cgcgtgcggg cgctcgcgac gggcctgcac tcgctcggcg tgcgccgcgg tgacaccgtc
    13561 gcgctcatgc tgcgcaaccg cccggagttc gcgctcgccg acgcggcggc catgcacgtc
    13621 ggcgccacgg cattctccgt gtacaacacg ctgagcccgg agcagatcgg ccacctgttc
    13681 cgcaatgcgc gcaatcgcgt cgtcgtcacc gagctgatgt tgctgccgcg cgtgctggca
    13741 gcccggaccg ccgacgtcga gcacatcgtg ctggtggatg gcgaggccga aggcgccatg
    13801 acactggcgg acctcgggcg acgcagttca cccgggttcc gcttcgagga tgcctggcgc
    13861 gaggtcgagc cgggtgacgt gctgacgctg atctacacct cgggcacgac cggcccgccc
    13921 aaggccgtgc agctcacgca cgcgaacgtc atgttcgagg cgcgggcgat cgcgacggcg
    13981 atgccctggg atcgaggtgg ccgcggcatc tcgtacctgc cgtcggccca cgtcggtgac
    14041 cgcacgctcg tgcactacgt gtcctcgatg tgcctgggcc ggaccatcac gtcgatcgac
    14101 gactaccgca ggatcggcga ggcactggtg tccgtgcggc ccaccatctt cggcggcgtg
    14161 cctcgcgtgt gggagaagat caaggccgcg ctcgaggtcg ccggcgtgcg cgcgcccggg
    14221 cagctgcccg aaccggtcaa ggcggccgtc cgcgagcgtg tcggactcga ccaggcggcg
    14281 tggtccgtca gcagcgctgc gcccgcatcg ccggaggtgc tgcgctactt caacgacctc
    14341 ggcatcgtgc tgtgcgaagg ctggggcatg tccgagctgt cctgcctggc gaccatgaac
    14401 ccgctcgacg acctgcgccc gggaaccgtg gggcgtgcgc tgccgggggt cgaactcaag
    14461 ctgctcgacg acggcgaact gctggtgcgt gccccgctcg tcatgaaggg atatcgcgac
    14521 gatcccgaga agacggcgga agccatcgat ccgcagggct ggctgcacac cggcgacgtc
    14581 gcgcagatcg acggcgacgg ctacgtgacg atcgtggacc gcaagaagga gatcatcatc
    14641 aactcggccg gcaagaacat gtcgccggcg aacatcgagt tccggctgcg ggctgcgagc
    14701 ccgctgatcg cgcaggcggt gtgcatcggc gatcggcggc cctacaacgt cgcgttgctg
    14761 gtgctcgatc ccgagccggc cgcgcgctgg gccgcggacc gcggcctgcc tgccgggctc
    14821 gacgcgctct gccaccggcc cgagttgcgc gccgcgctcg atgcggcggt ggcggctgcc
    14881 aacgagggcc tgtcccgcgt ggagcaggtc aagcgctacg cggtgatcgg cagcgagtgg
    14941 ctgccgggcg gcgacgagct cacgccgacc tcgaagctga agcgcaagcc gatcggcgag
    15001 aagtactcgg ccgagatcga gcggctgtat gcgtgagcag acgcggagcc ggacgctaga
    15061 aggcgagtga gcgcaggcgc atggtgcggc tggtcgccgt gctggcctcg agccgctgga
    15121 gcagctgcgt ggcttcgtcc accggcagtg ccttgctgag caggaatccc tggacctcgt
    15181 tgcactgctg ttcctgcagg ttcaacagct gttcgaagtc ctcgacgccc tcggccacga
    15241 cctgcagccc cagcgcccgg gccatggaga cgatcgcgcc gacgatcgtg gagcgccggc
    15301 cgaccgagtc gacggcgtgc accagcgtgc ggtcgatctt gagccggttg accgcgatcg
    15361 tgctcagccg gctcaggttg gaatgcccca tgccgaagtc gtcgatcgcg atggagacgc
    15421 cggtggcgcg cagttccgcg atgatgtcgc gcgtccggtc gtcgtcgcgc atcgcgagcg
    15481 actcggtgat ctcgatctcc aggttccgcg gttcgatccc ggtgtcgcgc agcacgtcgg
    15541 tgaccatgga gcagaatccg gggtgcgcga actgtgccgg cgagacgttg accgcaatgc
    15601 gacccggctc gaggtccatg tcgagccagc ggcggaattg ttcgcacgcg gttcgcatcg
    15661 cccattcgcc gagcggaacg atcagtcccg tctcctcggc catcgggatg aactcctcgg
    15721 gcgggacctt gccgagttcc cggttggtcc agcgcagcag cgcctcgatg ccggaaaccc
    15781 gacccgtgcc caggtcgaac tgcggctggt agtgcagcga gagttcgccg cgctccagcg
    15841 ctccctgcag ccggctctcc aggccgaagc gcgcctgagc gccgtccttc atcgtcgcat
    15901 tgaagtaggc gaaattgccg cgaccctggc gcttggccgc gtacatcgcg atgccggact
    15961 ggcgcagcaa cgcctcgtcg tcctcgctgt ccgtcgggta gatcgcgatg ccgacgctgg
    16021 ccgtgagggt cacctgggtc tggtccgcgt cgttcgaggc gagcatcggg cccgcgagat
    16081 gctgcgcgat cagggccgcg tcggccggtt cgcgcagccc gtcgacgagc accacgaact
    16141 ggtcgccgcc gaggcgcgcg acgtccggtc cggcgccacg cccggcgatg cagttcgaca
    16201 cgtcgtcgcg ctgcagtgcc tcccgcaacc gcaccgccac ccgccgcagc atctcgtcgc
    16261 cgacggtgtg gccgagcgcg tcgttgatgc gacggaagtt gtcgatgtcg aggtagaaca
    16321 gtgccatcga gcgcccgtgg ccccgtgcgt cgtcgaccgc ttcgcgcagc cgcgccagga
    16381 atttcgcccg cgtcggcagt cccgtcaggt cgtcggcgtc cgacagctcc gacaggcggc
    16441 ggtcggtgtt cgcgcgcttc tcgcccagcg cagccgccat ctgccggatc tcgtggggat
    16501 ggaagggctt ctgcaggtag aagagcttgt cggcgggcgg aacgcgccgg ccgatctcga
    16561 gcggatccac gtcggagtag gcggtgcaga ccacgatttc gacctgtggg tcgatttccc
    16621 ggatcttctg ggcggtccac acgccgtcgg gcccgggcgg catgcgcatg tcgaggaacg
    16681 cgactgcgaa cggctggttg gcggcgttgg cctcgcgcac ggcggccacc gcggcgtgcg
    16741 cgccggtgca atacgtcgcg tcgaacggct tgggtcgcgg cggctgcttc gccagcagcg
    16801 ccgggctgcc acccgacagg aacaggcggg tgcggagcgc atcgagagcc gcgcggtccg
    16861 cgctggcggg agacgcatcg agcacctgcc ggtacgcgtc gatgacggcc ggctcgtcat
    16921 ccaccacgag cacgcgcacg gcggcggacg gtccgggggg cggcgggctc atgcggctcg
    16981 ctccgtcgcc ggcctgacgc tgcgctgcac cggcacgacg acccgcagcg tggcgccttt
    17041 gccgcggctg ctttccgcgg tgatgctgcc gccgatcgcc tgcagcgcat tcgcgcacca
    17101 gtgcaagccg attccatggt tcgtcgcgcg ggacttggtc gagtagccat tctcgaacac
    17161 gcgcgtcagg tcctggtcgg cgatgccgac cccgtcgtcc gtgaagcgca gcnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncacgcgcg tcaggtcctg gtcggcgatg
    17341 ccgaccccgt cgtccgtgaa gcgcagcgtg agccgcgcgg cgccgtccgg ccccgcctcg
    17401 agccgcgccg agatgcgcag ccggccccgc tcgaggccgg cctcgtggac ggactccgcg
    17461 gcattctgga tcaggttctg gaacacctgc tgcagcgtga tacgcggcag cggcagcgca
    17521 ccgacgtcga tcacggaggt gtcgaattcg atcgcgagca gcctccgcag ggcgggcggc
    17581 accagctcga cgctgcggtc gagcagggac gcgagctgca cagtctcgat gaccgggccg
    17641 gcacgggact cgcgcagctg ctgcgcgagc accgactgga tcgcctcggc gcggcgggcc
    17701 accgcgtcgg cgtcgtcgag cgcagccgcg tgggtgcgca cgagctcgcg tacggcgagc
    17761 ttcaggaact gctcgaggtc gtcgcggcgc gccggctcga tcgatctgtt ctcgagctcg
    17821 gccaagacca tctcgatctc gccgagcggc gcgccacgca gcttctcctg cagcgaggcg
    17881 accgacaccg acagcggggt catcgcgttg ccgacgttgt gcagcacgcc gctcgcggcc
    17941 tgggcggcac cggcctcgaa cgactggtcc acgagactgc ggcgcgcgcc ggcgagcttg
    18001 tcgaccatgc gatcgaactc gcgagccagg atcccgatct cgtcccgacg ccgcacgtcc
    18061 acgcgcgacg tgaggtcgct gccctcggcg atcgcgacgg cgtgtcgggt caactggctc
    18121 accggccgga gcaccatgcg gcgcagggcc gccaggagcg cgagcaggac gacgatgccg
    18181 gcgacgaaga tggagatgag cgcgtagcgg acggcgtcgc gcccctgccg cgtgacggac
    18241 cggggcacgt cgatgcgcag cgtcacggcc ggccggccgt aaatgtcgtt gaggtcgagg
    18301 tacacctcgt tggcgtgcgg gccctgcagg atgcgcggtc gggtcgccgg gccgctcttg
    18361 gcaccggccg gatttgcagc ggaggccgcc agcacctgca gtttcacctg cgcctgttcg
    18421 gcgaggcgcg ccgcgacggc gggggtgatg acccgcgcca gcacgaccgc gccgtgatgc
    18481 ggtccgttgc cggcaccgtc gagcaccgga gcggtgacga tcatcgccgg cccgtgctcc
    18541 gtctgcacca ggcccttggc cggccggcct tcgcggatcg cgcgcgcaaa cggatggttg
    18601 cgccccagac cctcgccttc gaggaaccgg tatgcgagtt cctggtcagt gcccggcctg
    18661 aagccctggc gccatacgaa tcgcccctgg tcatccacgt aagcgactgc gtctaggccg
    18721 gccgcgacga tcgtcgcggg cgtcatgttg tcatcgatga accgggcgtt gcggccggcc
    18781 atgtactggt aggtctcgag ccagttgccc cagtccgcgc agaacgtcag cagctgggag
    18841 agttcgccgt cgatggcgcg cgacacgcgc tccatgtccg ttcgcgccag gtcgccctcg
    18901 agcttctcga agctggggag gatcaccgcg cgctgcacca cgtagtcggt cgcgccgtac
    18961 gccaggaaca gggcgcacag cagtacgacg accttcgaat agagactcac ggggcagcta
    19021 tccctggctg gttggcgttt ccgcgcaagt tagccgtgtg cccggcgcgc cgaggagacg
    19081 cagttcaccg aatgcccccg gttcccgagt cgtcggcgcc acgaaacgtg acacacgtcg
    19141 cgaagttccc gtgcgcggtc ccaggccacg cggcgcggcc gcccattgca tcgcagcatc
    19201 ccgcgcgttg ccccggccgg cggccggtcc ttcagcgtgc cggtagccac gcctatactc
    19261 gaccgcatgg agatgaacgg cattgcccac atccagctga cggtcaacga cttcgcggcc
    19321 tgccgggcgt tctacgcgaa gctgttcgag tggctggaga tgcgggtgat cttcgactcg
    19381 ccgcaggcct tctacggcgt gggcagccgc accggcatcg ccatctgccc tgcgcaggac
    19441 gagtttcgcg gagcgcgctt cgagcagcgt cgcgtcggcc tgcaccacct gtgcctgcgc
    19501 gcgcggcgcc gcgaggacat cgacgcgatg catgcattcc tgcagtcgat cggcgcccgc
    19561 atcgtccatc cccccgagga gggaccctgg gcgccggggt attactccgt gctgttcgag
    19621 gaccccgacg gcatacgcct cgaggccaac tacgtgccgg gcaagggcaa cctcgcggag
    19681 ggcagggcgc tgccgctcga cgtcgagcgg atggcgcgcg acgactgaac gacaggcagg
    19741 cgacggggga ggtcgggggg gcgttcagca cgcggaacga tcgcctgtgg caactggtga
    19801 tgagcggttc cgggcgcgat gccaccgatc aggcggtacg ctagcgcccc tgggtgtcag
    19861 gtgcggactc ggcgaggacg acatgcactt aagcaggctg tgcggattca tcatcgactg
    19921 caacacgccg gacctgcagt ccgccgcgcg cttctggggc gccgccctcg gcatgccgct
    19981 tcagcagctc ccggccggcg agggcgaaaa atacgtgcgc ctggtcgacc ctcgtgagcg
    20041 tctccacgtg gaggtgcagg cggtcacgca cccgagccgg gtgcacctcg acatcgaggc
    20101 ggacgatatc gaggccgagg tgcgtcgcct cgaggcgctg ggcgcgcagc gtgtcgagaa
    20161 ggtgcagacg tggtgggtca tgcaggcacc caccggccag cgcttctgtg tggtgcgggc
    20221 ccaatcgaag gacttcgagg gcggcgccaa ccgctggccc tgacgaggtg gtggcccgcc
    20281 tcggcgtgca gccgccgctg ggtcgttcga cattgatcca atccagggga gatcgacatg
    20341 gcaggcaatc ccgtccgctg gttcgagatc tacgtgcagg acatgacccg cgcgcgtgct
    20401 ttctacgaga cggtcctcgg ccgaaaactc gagaagctgg actccggcga cctggagatg
    20461 tggtccttcc cgatgcagcc cggccagtcg ggcatcggcg gagccctcgt gcgtgttccg
    20521 ggggtgcctt ccggaggcgg cggcacgctg gtctatttcg gcagcgacga ctgcgcgatc
    20581 gaggcctcgc ggatcgcgcc ggcaggcggg cgcctgcacc gggacaagat gtccatcggc
    20641 aagtacggct acatcgtgct gggcgtggac accgagggca acatgttcgg cgtgcactcg
    20701 acgaagtaga tgaatcggcg gcgaaccctg ctggccgccg gcctggtgct cgcgctcgtc
    20761 gcgcgagtgg ccggcgccat cgatgcaggt ccgctcagca tcaccgctgt cccgccagcc
    20821 gatccgcagc cggcgagtca cccgtgggcg gtttatctcg acgggtacat cgaccccggt
    20881 gccgccggcc gcctcgccgg gctgatgcag cgctcaggga tccgacacgc agtcgtgttc
    20941 ctgaattcgc cgggcggcag cctgctcgct gcgatggacc tcgggcgcct gctgcgggcg
    21001 ggcgggttcg acacgaacgt cggccggcgt gcgccggacg gccgcgagcc cgaggccggg
    21061 gtgtgctaca gctcgtgccc gttcgcgtat gccggcggag tccggcggtc gctgctgcga
    21121 ggatcggcgc tcggcgtgca ccggccccgg aacctcacgc ccgtggtgga cccgcaggca
    21181 ttcgagcggc gggtctccgc ggatgcgacg gactatctcg aaggcatggg cgtgaatccc
    21241 gtcctggcct ccatcatggc gcaggcgccg cacgacgcga tccgcctggt cggtcgcggc
    21301 gaggcggagc ggctcgcgct cgtcaacgac tgacgtccgc cctcgatcgt atgctcaact
    21361 gcaggctcga cgcctgctcg aaggaatccc aagcatgaag atcctgcgca ctcccgacgc
    21421 ccggttcgag caaattccag actatccgtt cgcgccgcac tatcaccagc tgacggacca
    21481 gctgcgcctg cactacgtgg acgagggtcc gcgcggggcg cctccggtcc tgatgatgca
    21541 cggcgaaccc acctggtcct acctgtaccg ccacatgatc gttccagtgg ccgcggcggg
    21601 attgcgggta ctcgcccccg acatgatcgg cttcggcaag tcggacaagc cagcggccaa
    21661 gtcggactac acctatgcgg gacatgtcgc atggatgcgc cactggctcg aggcgctcga
    21721 cctgcgggac atcacgctcg tgtgccagga ctgggggtcg ctcatcggcc tccggcttgc
    21781 ggccgagctg ccaggccgat tcgcccgcgt cctgctcagc aatggcggcc tgccggaggg
    21841 caccggcgct ccgagggcat tccgccggtg gcgcaggttc gcacgctaca gcccgttgtt
    21901 tccgatcggc aggatcgtgc aggccgccac gcggcggcgt ctgtccccgg ccgagatcgc
    21961 ggcgtacgac gccccgttcc ccgccaatgc gcacaaggcg ggggcgcgaa tcttcccggc
    22021 gctcgtgccg gtggagcccg acgacgtcgc ggtgccggac cagcggcgcg cgtggcaggc
    22081 cttcgaggcg tggaccaggc cgttcatctg ctgcttcagc gacggagacc cgatcacgcg
    22141 cgggggcgac gcgccgttcc tgcgccgcgt gcccggaacg aaaggccagc cgcacgtgac
    22201 gctgcacggt gggcacttca tccaggaaga cgacccggaa ggattcgtgc gggcgatcct
    22261 ctcgagctgc ggccgctgat cgctcgccgc cggcggcgct aagcggccag cggagcgctt
    22321 gccgcgactg gcaggcgcat caggtggtcg aacgccccga gcgccgcctt cgaaccttcg
    22381 cccatcgcga cgatgatctg cttgtacgga accgcggtgg cgtcgccggc ggcgaagact
    22441 cccggagccg acgtacctcc gcgtgcatcg gtctcgatct cgccgcgggg gttcagcgcg
    22501 accgcgcccc gcagccagtc ggtgtttggc acgaggccga tctgcacgaa gacgccctcg
    22561 aggtcgagtc gatggtgcgc cccgttgccg cgatcccggt agctcaggcc cgtcacccgt
    22621 ccgtcggctc cgtgcacttc cctggtctcg gccgcgacga gcacgcggac gttcggcagg
    22681 ctgcgcagct tgcgctgcag gacctcgtcg gcccgcagcc gcccgtcgaa ttcgatcagc
    22741 gtgacgtgcg cgacgatgcc ggcaaggtcg atggccgcct cgacgccgga gttgccgcca
    22801 ccgaccacgg cgacacgctt gcccttgaac agcgggccgt cgcagtgcgg acagtacgcg
    22861 acgccgtgcc cgcggtactc gcgctcgccg gggacgttca tctcgcgcca gcgcgcgccc
    22921 gtcgcaagaa tcaccgcccg gctgcgcaac cgggcaccgc tcgcgagttc gacggtcgcg
    22981 agcccggatt catcggccgg gtgcagcgcg gtcgcacgct gcaggttcat caggtccacc
    23041 tcgtacgagc ggacgtgctc ctcgagcgcc gcggcaagcc tggggccctc ggtcgcggtc
    23101 acggagatga agttctcgat gcccatcgtg tccatcacct ggccgccgaa ccgctcggcg
    23161 accagcccgg tgcggattcc cttgcgcgcc gcgtatatgg cggccgcggc gcccgccggg
    23221 ccgccgccga ccacgaggac gtcgaacggg tccttcgcgt cgagcgctgc ggcgtgccgc
    23281 gccggattgc ccgcgtcgag tttcgcgagg atctcctccg ccgtcatgcg gccgtggccg
    23341 aacggctggc cattcaggta gacggccggc acggccatga cgcgcctcgc gtcgatctcc
    23401 gcgccgaaaa gtgcgccgtc gatcatcgtg ctgcggatcc ggggactgag cgcggccatc
    23461 gcgttcagcg cctggacgac ctccgggcag ttctggcacg acagcgacac gtagacctcg
    23521 aactcgaagt cgccttcgag ggcctgcacc cgctcagcga cgccgtcggg cagccgcggc
    23581 ggatggcccc cggcctgcaa cagcgacagc acgaaagacg tgaattcgtg ccccatcggc
    23641 aggcctgcgt aacggattcg cggggtggaa ccggcgcgcg caacgctgaa cgagggggta
    23701 cgcacctggc cttccgggcc ggccgctcga cgcgtctcca gccgtatcag gctcgacagc
    23761 gacgcgacgt ccgtcagcag cgagcccaac tcgcgcgacg cctcggtgtc gtcgaggtgc
    23821 gccacgagtt ccaccgggtg cgagatccgc tgcaggtgtg cctgcagctg ctgcctgaga
    23881 ttcgagtcga gcatcgcgtg ccagcctccg gccgtgcgcc cggtcctggg gcgcagcgtg
    23941 ccctggtgat cgaaacaccc cgtccgtccc tggacggggc ggggggtcag atctttccga
    24001 ccaggtccag cgacggggcc agcgtcttgg cgccgggcgt ccagcgcgcc gggcagacct
    24061 cgccgtcgtg cgtggcgacg tactgggccg cctggacctt gcgcaacagc tcgcgtgcgt
    24121 cccggccgat gccgaggtcg tggatctcgg cgaccttgat cacgccttcc gggttcacga
    24181 cgaacgtgcc gcgcagggcc agcccttcct cctcgatcag cacgccgaaa ttgcggctga
    24241 tcgcgtgcgt cgggtctccg accatcgggt actggatgcg acgaatcgtc tccgacgcgt
    24301 cgtgccacgc cttgtgcgtg aaatgggtgt cggtcgagac gctgtacacc tccacgccga
    24361 tcttctggaa ctccgcgtac aggtcggcga tgtcgccgag ctcggtcggg cacacgaacg
    24421 tgaagtcggc ggggtagaag aagaccaccg accacttgcc gcggaggtcg gcgtccgtga
    24481 cctgcacgaa ctcgccgccg tggaacgcgg tggccttgaa tggcttgatg acggtattga
    24541 tcagggaagt catgtgcaag tctccgtggg gcagaggcgc gggatggcct cgttggagcg
    24601 gcaagatacg tagcgcacgg cttgagctcc aatcgattga aacgaatgga aagatagaca
    24661 ggctcgatca aaatggccgg ctgccgcggc gccgcaggcg tgcgttcggg ctcgtggcgc
    24721 ggacttgcta tccttggccg ggcgaccagt ccgtcgcagg ggggatacca tggacaagcc
    24781 gacgatcctg ctcaccggca tatccggctt catcgcgaag cactgcgccg tcgagctgct
    24841 gaacgccggt tacggcgtgc gcggcacggt gcgtagcctg gggaagtcgg ccgaggtccg
    24901 cgagtcgctg ggtcgtcacg ctccggtgtc gcgcctcgaa ttcgtgcagg ccgacctgac
    24961 ggcggacgcc ggctgggacg ctgccgtgcg tggctgcgag cacgtggtgc acgtcgcgtc
    25021 gccgtttccc gcgcgcccgc cgaagcacga cgacgagctg atccgcccgg cggtggacgg
    25081 cacgctgcgg gtcatgcggg cggcggcgga gggcggcgtg cgtcggttcg tccagacgtc
    25141 gtccatggtg gccgtgatgt acggccaccc gcgcacgcgc acggagccgt tcacggagga
    25201 cgattggacg cgcgtcgagt cccccgacgt caccgcgtac ggcaggtcga agacgctcgc
    25261 ggagcgcgcg gcacgcgaat tcgcggctcg cgagggagcg gcgctgcact atgcgtccgt
    25321 gaatcccggg ttcgtgctcg gtccggcgct cgaccgggac atcggcacct cggccgcggt
    25381 catccagatg ttcctcaagg gcaagtatcc gggcgtcccc cgcctgatga tcccttgcgt
    25441 ggacgtgcgc gacgtcgcgc gcatgcaccg gctggccctc gagaccagtg ctcccagcgg
    25501 cggtcgctac ctttgtgcgg ccgaggtcct gtcgttcctg gaaatgtcgc tggcgatacg
    25561 cgagaaactc ggcgcagagg cacgcaggtc gccgacccgc gagttgccgg actgggccat
    25621 caggctggtg gctctcttcg atggcgcggc gcgccaggcc gtgccggacc tcggcaggcc
    25681 gatgcacgtg gacaattccc gcacggtgcg cacgctcggc atgaagttca tcccggcgcg
    25741 cgaagcggcc gtcgcgatgg cgcgcagcct gctcgacctg catctcgcct gaaggacgac
    25801 cgcaatgagg ccgacgttat cggtgatcga gcctgcgctc gtcgatcgcg tgatcgacga
    25861 ggcgctcggc gtgctcgaaa agaccggcgt gctgatagag gatgaatctg cgctggcccg
    25921 cctccgaggc gctggcatcg ctgcggatcc ggcgtcgggc cgggtgctgt tcccgcgggc
    25981 ggcgatcgag cgggcgctcg cgaccgcgcc gtcgtcggtc acgctgcacg atcgggacgg
    26041 ccagccggcg gccgtgctcc agggcgacga cgtccacttc gttcccgcga gttcggcact
    26101 gcgcgtactc gaccgccgga cgcagcaggc gcgcgagccg ggcactccgg atttcgtgga
    26161 gtatgtgaag atcggcgacg gcctcaagaa catctcctat ctctccacgg cattcatccc
    26221 gaaggatatc ccgcaggacg tggcggacgt ctggcggctc tacctggtgc tcgcgcattc
    26281 gaagcgcccg atcgtgtccg gcgcgttcac cgcgtggggg gtgccgcgca tggggcaggt
    26341 catggcgatg ttccgccgcg accgcgacga cctggtgcgg cgcccgatgt cgatgttcac
    26401 ctgctgcccg aacacgccgc tgcgctgggg cgaggacccg gtggcgaaca tcatggactg
    26461 cgccgagtgg ggcatcccga tggagatcgt gccggtgctg ctgctcggca tgatctcgcc
    26521 cgtcacgacg gtgggcgcgc tggtgctgca cactgcggag gtgctgtccg gacttgccat
    26581 cgcgcaggcc gtgcggccgg gcacgcccgt ggtcttcggc ggcgcacccg cgtccttcca
    26641 catgcggctg atgaccaacc cgatgaccgc ggtcgaggcc ctgcagctct attgcggcta
    26701 cgcgcaggtc gcaaagcggc tgaacctgcc ttgccagggg tacatggcgc tgtcggatgc
    26761 caagttcaac gatccccagg ccggcatgga gacgggggtc ggcgcgttcc tcgccgcctg
    26821 tgccgggatc aactcggtct cgggccccgg gatgctggat ttcgtcaact gcttcagctt
    26881 cgagaagctg gtattcgacg acgaagtcgt cgcgcacgcg aagcggttcg tgcgatcggt
    26941 cgaggttcgc gaggacctgc cggccggcga gctcatcgcg gagctggtgc gcgaccacca
    27001 cctgctcacc tccgagcaca cgctcgcgca ctggccggac gaactgtacc tgccgggccc
    27061 cacgctcgac cgcatgaact gggaccagtg ggccgaccac ggctcgcgcg actggcgcgc
    27121 ccgcaccaac gaggtcatcg acgagctgct ctcgaattac gaggtggagc cgctcgatgc
    27181 cgggctgcgc gccgagatcg aggggctgat cagcaccacc tgcagggaag tgcccggcgt
    27241 ggccctcccg gcgttcacgg gggacgcgca tgcctgatta cgcgctcatg aaccagtcgc
    27301 tcttcgaggg gcaccacgac gccgtgcgcc gcatgaccga ggaggcgctg gcggagggcc
    27361 ggcccccgat ggaagtgctg gaggacggcc tgatcgcggg catgcgcgtg gtgggcgagg
    27421 atttcaagca caatcgcatc ttcgtgccgg aagtgctgct cgcggcgcgg gcgatgaagg
    27481 ccggcatggc ggtgctgaag ccgctgctcg gctcgaagca ggacggcgcc tctgcactgt
    27541 cggagaccat cgtgctcggc accgtcaagg gcgacctgca cgacatcggc aagaacctcg
    27601 tcggcatgat ggccgagggc gccggtttca ccatcgtcga cctcggtacc gacacgtcgg
    27661 ccgagcggtt catcgcggcc gcgaaagagc acggcgcgaa gatcgtcggc atgagcgcgc
    27721 tgctcacgac cacgatgatc tacatgcgca cggtggtgca gaagttcaag gaagccggca
    27781 tgggcgatgt gaagctctgc atcggcgggg cgcccaccag cccggactac gcgaagcaga
    27841 tcggcgccga cggctacgcg gcggatgcgg cgagcgccgt cgagctgttc aagcggctca
    27901 tcgccgagca cgcggccgcc gcgaagtgac cgtgccgggc gcagcggcgc cgtcgggcgg
    27961 cgccaccctc accatcgatg ggctcaccgg cctcgcgccg gcggggcaga ccctgttcga
    28021 ctgggcggag tcgcgcggca tcgccgtccc gacctcgtgc cacaagcagg gcaagtgccg
    28081 cgagtgcctg gtggagatcg agactggcgc ggaatgtctg agcgagcccg ccgagcagga
    28141 attgcacctc gagggccgct tccgcctggc gtgccggacg cgcgtcgtac aggacgggca
    28201 ggtcagggcg cacaccttgc gccgcgggtc gatgcgcatc gagacctcgt ccgacgccga
    28261 agccatcgac ggtgtcgcgc tggatccggc ggtcacgcgg gacgggccgc gcatcctgct
    28321 cgacggtgaa ccgatcgcgg aggccggtgg cccgctgcac ggcgtcgcga tcgacctggg
    28381 gacgacgacg atcgcattgc ggctgcacga cctcgggacg ggtcgcctgg ttgcgacgca
    28441 ctccttcgag aacccgcagc gcttcggcgg gacgaacgtg atgtcgcgca tccactacga
    28501 cggcacgcac cgtggccggc tgctgcagcg cacgttgctc ggctacctga cgcacgccat
    28561 cgagtcgctg ccggtggatc ctgcgaccat ctacgagatc gtcgtggccg gcaacccgac
    28621 catgcgcgac ctgctgttcg ggctcgacgt gcaggggctc ggcgttcttc cgtaccggtc
    28681 gacgaccgaa gcggcactcg cggacggacg cgcgacgacg accagcctgg cagcgacggc
    28741 aagacgcctg cgcctgcccg cgcatccatt ggcccgcgtc tacgccctgc cgctcgtcgg
    28801 cagccacgtc ggtgccgacg cggcggcctg cctgctggcg accggaatcg ccgaaggaac
    28861 cgggatcagc gccctgctcg acatcggcac caacaccgag gcgttcttcg gcaatgcgga
    28921 ccggatgctc gcggcatcgt gtcccgccgg tcccgcgttc gagggtggtg gcgtggggtc
    28981 cgggatgccg gcgctcgacg gggcgatcga gcgcgtcgcc attgccgacg acgggtcgat
    29041 cggcatccgc accgtgggtg atgccgcgcc gatcggtgtc tgcgggtccg gcctcgtcga
    29101 cctgctgtcc gaactgcggc gcaccggccg gatgaacgcg cagggccgct tcctcgatcc
    29161 cgacgagccg atcaccctcg atggcgaagg gcgcgtgtcg gtcagcgaga tcgacgtgaa
    29221 cgaactcgcg caggccaagg gcgcgaacgc cgccggcatg cgcgtgctcg cggacgtcca
    29281 tggcgtggac ctcgcgcggg tcgatcggct gtatctcgca ggcggtttcg cgcggcacct
    29341 cgacgtggac gccgcgtgcc ggatcgggct gatcccggac ctgcctcgcg agcggatcgt
    29401 gaaggtcggc aatgctgcgc tggccggcgc gtcggtcgcg ctgctctcgc ggcgtcgtcg
    29461 ccaggacatc gagtcgatcg tgcgccgcat cgagctggtc cgactggagg cgcatccgag
    29521 attcttcgac ttcttcgtgc agggctgcca gttcgagcct ttcggggccg cgccatgacc
    29581 gttgccagca tcctgcaggt gtcggggagc gacgcggcgg cggccgtgtc cgacgcggag
    29641 ttccaccggc tgctcggcgt cccgcgcggc cgcgaggtcc cggtcgaggt cgccgagcgc
    29701 gccggtctcg cgcgacgctg gtacgaggag cacgggcgcc cgttcgtcgc gacgagacga
    29761 ttggctctcg ccgcgatcag ccacgatgcc gtaactctcc gcagtggcga cgtgctcgcg
    29821 ggacccgcgc tcgcggaccg gctgcgcgat ggcgaagcgc atgcattggt ggtggccgcg
    29881 ttcagcgccg gccgcgaagt cgcggacgag gcggcgcgcc tgtgggccga cgcgcgtccg
    29941 gacgagtcgt acttcctcga ccggttcgcg gcagcggttg tcgaacagct cgtgtggcgg
    30001 gcttcggcgg tgctgtgcgc ggacgcgacg gcgaccggcg agggcctgct ccagcacttg
    30061 tcgccgggct gcggtcgctg ggagctctcc gcgcagcacg cattgatggc gctgctgctc
    30121 gggacgactg gccaccacgc cgacgcgtgg tcgatcggac cgatcaggct gctcgagtcc
    30181 ggcgcattgc atccgcagca ctcgctgctt gccgcgctcg gggtcacgcg ccgtgctatc
    30241 gtcgcgacgt ccgagtcggc gtgccgcagc tgcgacctgc gacattgccg gtatcgccgg
    30301 gccgcgtacg cgcgcgacgc cgctgaacca gggacgaccg aatgacgcag ccgtcattgc
    30361 tcgatgcgat caagacccgc gtgctgctcg gcgacggggc catgggcacg cagttgcaga
    30421 acgcgggcct cgagtccggg cattgcggcg aagcctggaa cgtcgatcat cccgaccggg
    30481 tgctcggcat ccagcgcagg tatgccgagg ccggctccga cgtgctgctc accaatacgt
    30541 tcggggcctg ccgcatcatg ctcgaccgcc acggcgaggg agctcgtacc gcggacatca
    30601 atcgcgcctc ggtggcgatc gcacggcagg cgctcggcgg gcgcgggtac gtgctcggcg
    30661 acatcgggcc gttcggcggc ctcatggagc cgtatgggga catcacgcgc gccgccgtgg
    30721 agagcgcctt ccgcgagcag gcgcgcgcgc tggtcgaagg cggcgcggac ggcatcatca
    30781 tcgagacgca gacggcgttc gaggaagtgg agattgcgat cgcggccgcg cgcgaagccg
    30841 gcgcgaaggt cgtgatcgcg tcgttcgcgt tcgaccgcat ggcggacgag gacgacgtgc
    30901 gcacgatgat ggggatcagc cccgagcagg ccgccgaatt catggcccgc cacggttgcg
    30961 acatcgcggg cctcaattgc ggaaccggcg tggacatgcg cttcgcggcg gacatcacgc
    31021 ggcggtatcg ggcgacctgc gggctgccgg tgatggcgca gccgaacgcg ggagccccgg
    31081 tcctcgagga catgaaagtg ctctacaagc agacgccgga agagatgggt gccggactcg
    31141 ccggcttgct ggcggccggg ccgcgcatcg tcggcgggtg ctgcggcagc acgccggcgc
    31201 acatcgcgcg attccggcag atcctcgacg gcggggtggg aggtcggcag gcatgaaggt
    31261 gacgattccc gtggacgagg cggcggtcga tgcgtacctg ccatcggcca agcccgacgg
    31321 atcgggggtg ggcgtcaact acgccgacca ggtgctgaag ctcctgaaag taacgctcga
    31381 ggacggccgc aagctcgccg ccaggaggcg cggcctcaag gtggcggtca cgatcgggga
    31441 gcagtcgggc cacgcgatca tgcgacgcat cgacgacggc cccgacacgc gcgtgatctt
    31501 cgagcgggcg ctcgccgccg cgctgcgcga ggcgggtgcg acgttcagcc gcgagccggg
    31561 cgcgatccac atcgaagcct gagtcggcag ccgcacccgg ccccggggcg gtagctggca
    31621 attcgacggg caccgaaggc ccgccgcggt gcgcgaacga aaagaattcg caattattcg
    31681 acagccagtt ggcgataatc cgccgctggt cacattcacg gcggcccgcg cggcgtatct
    31741 agacgccatg aacggcacca ggcttgcttc gtacaagctg tcgcaggacg ccggattcgc
    31801 gccgaacccg ttcttcggca cgctgacgct ggcgacctgc atgcccgaca tgcgcctggc
    31861 ggccgcgttg gacgactgga tcgcgggctt cacgtccgag gcgttgtgcg gcgatgccgt
    31921 cggcgccgag cggttggtgt tcctgatgcg ggtcgaggag gcgctctcga tcgccgagta
    31981 ctactcggac gcgcggttcg cccgcaagat cccggtgccg aacggcgcgc ccgaggtcgg
    32041 ccgccatggc gacaacatct accagcccag gcgcgcggac gcgggcgagc cggaacactt
    32101 cgcgcagttg cccaatccgc atcactggga ccatacgcac cgccgcgagg acgaacgcag
    32161 caagcggctg gacctgcgcg gacgtcgtgt gctgctggca accgagttcg tgtacttcgg
    32221 ccgcgaggcg ctggtcgtcc ccgaattcgc acggcccgca ctgccccggg gacacgctcc
    32281 ggccggctcc tcgaccgcgg atcccgaatt ggcacgggct ttcatagact acgccctcgg
    32341 ccgggcgagt cgtcccgtgg tcgcgccgcc gcacacgtgg ccggccgggg acgacagctg
    32401 gcgtcattga acgacggtcc ggcgacacgg aggccggtgg cgagcggagg tgtcgggcga
    32461 tgcgaagcat cctgggctgg ttggcagcct tcatcggcgg tgcatcgggc tggtggctcg
    32521 ggcggcacgt gggcctgggg acggccgtcg tgctgagttc gctcggtacc ggtgcgggca
    32581 tgtacttcgg ttatcgctgg ttcgacgaga acctgcgcta gcaggttcca cgcggtcgcc
    32641 cgaggacgtc ggccggcttc gagtgccgct gctgcagtaa gatgacggcc catgcgggcc
    32701 ggcatccggt cgcgcacctg cacggattcc ggtcgatgag cattcacccc acggccatca
    32761 tcgaggacgg cgcgcagctc ggcgcgggct gcgtcgtgca cgcccatgcg atcgtcagca
    32821 ggcattgcgt gctcggagaa ggcgttgtcg tgcatccctt cgcggtggtg ggaggggatc
    32881 cgcaggacct caggttcgac gtcgcgacgc ggactggcgt gcgcatcggc gcgcgcaccg
    32941 tgatccgcga gcatgtcacc atccatcgtg cgacgaaagc cgacggcggg accgacatcg
    33001 gcgcggactg ctacctgatg gtcggcagcc acgtcgcgca cgattgtcga atcgctgacc
    33061 gcgtcatcat ggcgaacgcc gtgctgctcg ccggccacgt gcagatcggc gnnnnnnnnn
    33121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    33181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngaccgcgtc atcatggcga acgccgtgct
    33241 gctcgccggc cacgtgcaga tcggcgaggg cgcgttcatc ggcggcggtg cgaccgtgca
    33301 ccagttcacc cgcatcggcg aaggcgcgat gatcggtggt gcggcgcggg tgtcgcgcga
    33361 cgtcccgccg ttctcgatga ccacggagcg ggacgaagtg gtcggtctca acgtcgtagg
    33421 actgcgtcgg cgggggctgc cgaaggccac gctcgaggaa ctcaagcgcg ccttccgcga
    33481 ggtgaacgtc ccggtcggca acatgcggga gattgcagcg gcagcgctgg ccggcgggtc
    33541 ctacgcgacg ccggaggcaa ggcgctacct ggagttcttc gctgccggcc ggcgcagctt
    33601 cgtgcgcgcg cgtcgcgcag gcgcggccct ggactcggac gagtgatcgg gccggcgcgg
    33661 acgcagcgag gggggaacct gatggccatc gagggactcg acgccgccgt ggcggccgcg
    33721 ctgatcggca tcgacaccgt ctggggcggc gacgtgatga acccgtccgg gacaggccgg
    33781 ttcatcgccg attcgtggtt ctcgggggag ccgctgccgt cggcctacac gcgtccggag
    33841 gcggcggtgc tgcgttcgag cggcggagtc ggggcgcgga tgccggatgt cccggcagtc
    33901 gaagcctatc tcaaggcgat cgaactgccg ggaaccatcg cacgcgtcat tgccggcggg
    33961 cgttcactgg gtggcttgcg cggcgcccac atcgagggcc tgggcctgtg ctgcgaggtg
    34021 atgctggacc tcgcgctgga gatcctcggg cggggccccg ccgtgcccta cgagcgttgc
    34081 gttcgaaccc tgaccggctc ggagccggag ccatcgcgac cggacgacaa ggtgcgccgg
    34141 gtcgcggagc tgctcggtaa cgggaccgga tcgccgccac gcggggccgc actcgtcgcc
    34201 gcggtcgacg agtggcgcgc ggcgcgacgc gtgccgcggg gctccatcgc gagcctgggc
    34261 gaccgctgca tcgccgagta cgacgccttg acgaagcgca acctgttgcc gcacctgccg
    34321 cgggacatgg ctgacgtgcc gcgcgccaac gtgcggttcc tgcccatcga gaacgcgtgg
    34381 ttctccggct cgatgaacta catcggccgc gcgcgcaagc ccgacgggac tccggaatac
    34441 gaggcgacct acgagctgaa tgcggcgctg gagatctcgg tgccggaatt cgcgcagctc
    34501 gtgagccacg aggtcgtgcc gggccacgtc acgacgttcg cttacctgca gaacctgtac
    34561 gtgcgtggca tggctggctt cgaggtcacg gtgcaggcaa tgaacacccg cgactcggtg
    34621 ctcgccgaag gcatcgccaa caatgcgata ttgatcgcgc acggagtcac cgagctcgag
    34681 gacctgcccg atcgcgagct cgagctcggc gtgctgctcg cgctcctgca ggacgacgcg
    34741 aagaaccagg cgtcgtacct cacgtggaac gacaaggtcg cgcagtccga ggtcgcggcg
    34801 gtcctgcgca gggatttcct ggtctcggag gagcgcgcgg acaagctctc gggtgcctgg
    34861 ggacgccacc cgctgctcgg caggatgtac ctgcctgcgt accgcgcggg cacggagctc
    34921 gtggcgcggt tgcgtcgccg ctatccgccc ggggtgatcc tcccggcgct ctatggctgt
    34981 cgcgggtatg cggactcgca cacgctggaa ggactgctcg agggtcgcta cggccccgga
    35041 tgacggcggt cgcctatcgt cgcagcggca gccacgtcca gtaggtgccg cagcgccaga
    35101 cccactcgag cggtcccatg cggtagcgga gcagccatgc gtggctcgcc aacgcgagca
    35161 cggcgaagac cacgcacgcc agcaccgtca attcggggcc gctcaagctt ccgtagagcc
    35221 cgaagcccca aggctccgcg agcgcagcca tgatcagcga ctggcctacg tagttcgtca
    35281 atgccatgcg gccggccgat tccatcggcg cataccacca gcgccgcttg cggaggtgct
    35341 cgttaagcat gacgcgcgcg ccgaacccga tggcaagcgg gaaggcactc gcgacgtgca
    35401 gccactccgg ccagcgccag tcggtcacgc catgcatctc gcgggactgc agaaatgtcg
    35461 ccagcacgct catcggcagg ccgatgccga gacccacgat cgatacgcga cgccaggtcc
    35521 gcggcgtcgc acctgcgatc acaccgctgc gcaccagggc cgctccgatc atgttgagcc
    35581 ccagcgtccg ccacagcagc acgaccccga accagaccag caccagccag tgctgctgcg
    35641 catggagttg cagcagcgcc cggaacccgc cgtccctgcg cgccgcatat tccgcgggcc
    35701 aggcccccgc atccgcggag cgcatcgccg caaggtcgcc caacgccagc gagtactcac
    35761 tcgcgacgct ccgtccccaa cctcgcgggt cgccgcacag caggaggacc gcgatcgcga
    35821 tggccatgac gagggcgacg accaacaggg ccgcgcgcca gttcctgtcc cagagcagcg
    35881 ccacgacgac cgtgaatgcc accatggcga ttccggtgac tcccggcgac acgcggccca
    35941 gggagccgaa ctggaatccc cacacgaccg tggtggccac cagcacgatc ccgatgcgat
    36001 agaggttcct agtgctcagg cggcgcgcgg catacaggca caggcccgcg atcgcgtacg
    36061 gcaggaggac gtccgccggg tacagcagca gcgcatgcag ggcgccgaac accgcgagca
    36121 tcgcgatccg gcgcaggtag atggcaggga agcccggccg tgcgaccgcg ctgcgtccgc
    36181 tctgcagcgc gaagcccatg ccgaagagca gcgagaacag cgtgatgaac ttgaggtcca
    36241 ccaccgcatc gaagagtgcc cagtcgagct ggccgagcag ttcgccgtgc gcgcccggca
    36301 cagggaacgc cgcgacgtcc cagggcagga acatctgccg gacgttcgcc accaggatgc
    36361 cgagcagcgc gatgccgcgc acggcatcga tcgacgcgat gcgctccgac ggaccggtcg
    36421 gcgccgcgtt cagccccatc gcagcgttcg ggcgaacact gcgtccaccc agcggtccgg
    36481 caagcgtcgc aggaccttgc gcaccccgct gccgtgcccc ttgcagtagc gcgtccgggg
    36541 tcgcgccgcc gtgaatgcat ggacgacgtc cttcgtcacc acggacacgg gcgaggcgtc
    36601 acgcgccgac tgcgccgcga actgattgag tgtccgcgtc agtggtccat acagctcgac
    36661 gaggcccggc ggcggggctt tcaggttcgc ttcgaatgcg tccgcgccct tgctccagat
    36721 ctcggtcgcg atggacccgg gctcgagcag cgacacctcg atgccccacg ggcgcagctc
    36781 ccggcgcagg gcatcgctca gcgcttccat cgcgtgcttc gacatggagt acggcccgag
    36841 gccgggcacg gtgccgaagc cggcgatgga cccggtgttg aggattcggc cgcgcgcccg
    36901 gcgcagcagc ggcaggaatg cctgggtcat tgcgacctga gcgaagacgt tgacctcgaa
    36961 ctgccgacgc agttcgtcga cgggggtgca ctccatcggc cctgcgacga cgatgccggc
    37021 gttgttgaac aggccccaca gtccacgttc gccgacggcc gccccgacgg tcgtgactgc
    37081 ggaccggatc tggtccgggc gggtgacgtc gagtcgcagt gtccgcagcc tgccatttgc
    37141 gtccgccgcg aggtcgcgct cgagcgtctc ggccgcggaa tcgctgcgca cgcatgcaaa
    37201 gacgcggaat ccggccccga gcagcgcgcc tgcggacgcc ttgccgatgc cgctcgacgc
    37261 gccgctgacg agtatcgctt tgtccgaatt cgaggtcatg aacctgctcc cgcgtacgtg
    37321 gactgcggtg cgtcttgtgc gccgcgcgag tcgggtatcc taccccgagg cgcgcaacgc
    37381 cgcgttgcag gcaggtcggc aggagttcac atgcgctttg tgtcaccgtg caccgtcgcc
    37441 acgctggcgg ccgtcggttt cgccatgcag gcgaacgctg gcgacgtggt cgccgcgaag
    37501 ccggtagcgg cggcggaggc cgcttttctc gatttcctcg atgcgcacgg cgaactcggg
    37561 gtcgtcgagt cggggctgca ggagcgctac ctgggacgcg atcgcgcgca gtggcaggcg
    37621 ctgtcggact cgcgccgcaa tcgcctcgat gcagcgctcg ggcggctgaa gcccctgcag
    37681 ctccggcctg cggacgagcg cgcagtacag gccatgctcg cctcgctcgc ggccctggca
    37741 agcgaggatg cggtgtcgaa ctcgccagcc accagctgca aggacgccgg ggaacgcgac
    37801 ctcgactacg cgcgactgcg tgcggcgctg gtcgcctgct acgtcgagta cggcaacgcg
    37861 ctccggttcg aggacagcac gatcgatcgc ggctcggcgc tgcaattgct gcacgtcatc
    37921 gacgagccgg cgcggcgccg tgccgtcttc gacgccttcg ttccgctgtg gaccgcactc
    37981 gacgggcatg gcgagccgga cagtccctac cgccgcatga tcgctatggc ggcggaggct
    38041 gcgcgcaagc agggttcgcc gatcgatgcg gcagcccgcg ccctcggcgt gcgcgagacg
    38101 gacgtcgaga actggctcgt cgaggtcctc gacgcgtggc ggcaggcgac cgggccgcag
    38161 ctggtcgagc cctgggacta tcgctacgtc aacagcgcag ccaatcgcga gcttgccgcg
    38221 aggatcccgc cggagtcgct ggtgccagcc aaccagcgct tttaccagga ccttggcgcc
    38281 gacctcgcgg cgctgggtgt catgtacgac ctggagccgc gagccgggaa gtcaccactc
    38341 gcatacacgg atttcgtgac gcgaggtcgc accacgggcg gccgctggca ggtgtccgtc
    38401 gcgcgcgtgg tcgcgaccta tccgacgggc gggctgtact ccctgaacga actggtgcac
    38461 gagaatggtc acgcggtgca cattgccgcc atccacaacc ggccggcgta catggactgg
    38521 cccgacacgt tgttcaccga ggcgtttgcc gacgtgccgt cgtggagcgt ctacgagccg
    38581 gcctggcagc gccgctacct cggggccgcc gcggccgagc cggcgtcgct gcgcgcgctg
    38641 ttcggcgacg tgatgctcga cgtggcgtgg gcgctgttcg agctgcggct cctgcgcgat
    38701 ccgcgccagg atccgaacgt gctgtggacc gacatcacca gccgctacct gcacgtcgcg
    38761 ccgcacccgg aggtgccgtg gtgggcggtg cgcgtgcaac tcgtcgacga gcccgggtac
    38821 atggtcaact acggcctggg cgccgtgttg accgcggaga tgcgacaacg cacggtcgac
    38881 gccgtcgggg tcttcgacgc cggcaacccc gggtggtacg cgtacctgag ccggcagctg
    38941 ctgcaatacg ggtccgaacg cggcacgcgc tcgctgctgc aggacctgct cggccggccg
    39001 gtgtccccgg ccgcgctgat ccgccaggtc gcgcgcagcg gtgacgccgc ggcgtccggg
    39061 tcgacggtca ccgggaacca ggggacattc ccctatttcg cgagttcgaa ataggagaat
    39121 gtcccctggc ggccgccgcg cagcaccggc gcactacgcg taggcggcgt accgcggctc
    39181 gtacatctgc gaccgcatgt attcgtacag gtcgtccggg cgttccatgc ccgcgaggcc
    39241 gcggctgaac accacgtcgg cgaccgcggc ggcgatgtga gccgacacgt cgcgcacccg
    39301 gttcagcggc ggatacaggc tgccctgggc gaggtcggat tcgttcacgt gccgggcaag
    39361 ctcgcgcgcg gccgccatga acatctcatc ggtcacgcgc gtggcgcgca ccgcgaccac
    39421 gccgaggccg acgcccggga agatgtagga attgttgccc tgccgaggca cgtagcggcg
    39481 cccatccagg gtcaccgggt cgaaggggct cccggaggcg aacaacgcgc gtccgcccga
    39541 ccaggcgtac gcctgctccg cggtgcactc cgacttcgac gtgggattcg acagcgcgaa
    39601 cacgatcggc cgctcgttcg ctctcgacat ctcctcgatg acgtccttcg tgaacaatcc
    39661 cggcgttgcg cccacgccga tgatggccgt cggctgcagc gccttgaccg cgccgaggaa
    39721 atccgggacg ggcgcgtgct cgtgagcgta tggaatcttg tgcgccgcaa gtccctgccg
    39781 ccccgcgacc accagcccct tggaatccac catccagcag cggcggcggg cttcggcgat
    39841 gcccatcccc tcggcggtca ttgcggcgac gagcaggtcg gagatccccg tggccgcttc
    39901 gccggcaccg aggcagagga aggtctgctc cgacatcctg cgtcgcgtga cgcgaagcgc
    39961 ggagaagatg ccggcgaccg tgacggacgc cgtcccctgg atgtcgtcgt tgaagcagca
    40021 gacccggtcg cgataccgct cgagcaggcg gaatgcgttg tgattcgcga agtcctcgaa
    40081 ctgcaccacg atgcccggga agacctgctg ggtcgcctcg atgaactcct cgacgagctc
    40141 gtcgagcgcc cctcccgaca ggcgacgctg gcgcaggccg agatacagag ggtcccgcag
    40201 cagcgcttcg ttgttggttc cgacgtcgag catcaccggc aggcagcggc gcggatgcag
    40261 gccggcgcag gccgtgtaca gcgcgagctt gccgatcggg atgcccatgc cgttcgcgcc
    40321 gaggtcgccg aggccgagga tgcgctcgcc gtcggtcacc acgatcattg ccgcctcgcg
    40381 gtgcggccag ttgcgcatga cctgggcgat gcggccgcgg tcagcggcgg agatgaacag
    40441 gccgcgcggc cggcggtaga tgtgcccgaa ctgctggcac gcgagcccga cggtgggcgt
    40501 gtagatgatc ggcatcatct cgtcggggtt ctcgacgatg acgcggaaga acaatgcctc
    40561 gttgcggtcc tgcagggcct cgatcaggat gtacttcgcg agcggtgtct gcagacggtg
    40621 gaagttctcg agcacgcggg cgacctggtc ctcctgcgtg cagacgtgcg gcggcagcag
    40681 gccgcgcagt cccagcacgt cgcgctcgtg ctcggtgaag gccgtgccct tgttgagggt
    40741 cgggtcgcgc aggagggcgg ttccgcgcgg gaaccactcg ggaagcggcg ggggcagcgt
    40801 gcgctggacg cgactggacg gcatggcg
//
LOCUS       DAOMFD010000002        39120 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_108_length_39120_cov_39.120927, whole genome shotgun sequence.
ACCESSION   DAOMFD010000002 DAOMFD010000000
VERSION     DAOMFD010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 39120)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 39120)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39120
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_108_length_39120_cov_39.120927"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            342..680
                     /locus_tag="PL152_00195"
     CDS             342..680
                     /locus_tag="PL152_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016654695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQR47718.1"
                     /translation="MKLITAIIKPFKLDEVRQAIADIGLQGVTVTEVLGFGRQRGRTE
                     FYRGAEYHSGLLPKTKVEIAVEDTVVDPVVEAISNVARTGRTGDGKICVTNLRQAVRI
                     RTGERGAEAV"
     gene            739..2139
                     /locus_tag="PL152_00200"
     CDS             739..2139
                     /locus_tag="PL152_00200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HQR47719.1"
                     /translation="MNTLTAARPGLRAAALALGTLLAPALAFAADPVPDKGDTAWMLT
                     STMLVILMTIPGLALFYGGMVRSKNVLSLLMQVFMTFCLVAILWAVYGYSVAFTSGNA
                     FFGGFDRAFLQGLTSSSVAATFSKGVYIPEYVYFVFQMTFACITPCLIVGAFAERIKF
                     SAVLLFMVLWFTFAYLPIAHMVWFWAGPDAYTDPTAGAAATAGAGFLFQKGALDFAGG
                     TVVHINAGIAGLVGALMIGKRIGYGSAAMAPHNVPMVMIGSSLLWVGWFGFNVGSNLE
                     ATGFAGQVFANTLLATAAAALAWSFAEWIHRGTPTMLGAASGAVAGLVAITPACGWVG
                     PMGSIAIGLIAGLVCLWAVVSLKQKLGYDDSLDVFGVHCVGGILGALLTAVFCSPALG
                     GTGVYDYVANAVGDYSIAAQFKSQLWAVLVSLAWSGLVAAISFKVVDLVVGLRVAEDQ
                     EREGLDTVSHGERAYN"
     gene            2304..2741
                     /locus_tag="PL152_00205"
     CDS             2304..2741
                     /locus_tag="PL152_00205"
                     /inference="COORDINATES: protein motif:HMM:NF024901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4124 domain-containing protein"
                     /protein_id="HQR47720.1"
                     /translation="MAGLVLSAAAAAGDVYVTKDAQGNLIYTDTPLTVPAKKVDIHSD
                     STNPETAEQRYTSEMSQYAKDNQAAAKSQSQQAAQQQAAQDTAADQAKRCVEARQTYQ
                     TYMNSWRLYETGPNGERTYLTSEQIDKARADAKQAMDQFCSGQ"
     gene            2749..3654
                     /locus_tag="PL152_00210"
     CDS             2749..3654
                     /locus_tag="PL152_00210"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR47721.1"
                     /translation="MVTAAGFVGLGAMGRGMARNLHRAGLLGSVWNRTHPVAEQLASE
                     LGVTVAASPAALAAASDVVVLCVSADADVLAVVDAMLPSIRPGTIVIDCSTVSSDTAR
                     AAAAKLAGCGADFLDAPVSGGVEGARDGTLAIMVGGSPEAFERAAPVLQAMGRTVTHF
                     GPSGAGQAAKATNQIMCAGIIQAVAEAMAFAKAEGLPLAQLVETLGKGAGSSWYFVNR
                     APNMVRGTYPAGFRVRLHDKDLRICRDMAARHGVELSVVESALGHYRRLIEQGHGDED
                     ISTIFRLTDALFTHEGAGDVSPKPR"
     gene            3651..4736
                     /locus_tag="PL152_00215"
     CDS             3651..4736
                     /locus_tag="PL152_00215"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HQR47722.1"
                     /translation="MSEVPAAPAGRSDYARIGGLLLAALLAYLVWSVVEPLWRPLVWA
                     ALLGSLLAPLNMRLAVRLGGRKRLASVVTTLATVLLFLVPVGIIAGAVTTQAAQLLGR
                     LNSGAAALNPQSFDIGHVPWLARPLEWLSEHTSISAEQVQGWTVGAAKHVLQSIASSG
                     GNLVLGALGTVLSFALMLFVLFFVLRDGPDVARKVVGMLPIEERRRSRLWQHLVEVNR
                     AVFMGIGLTAVVQGVLVGIGFWIAGLPSPLLFGVLGAFCALVPLIGTALVWVPGAAFL
                     ALHGDYGHAGFLTAWSLVVVGSVDNFLRPMLISGRAELPTLAVFVGVMGGLSAFGFIG
                     LFIGPIVLGLLVALFRFEHEERAAAAN"
     gene            4809..5750
                     /gene="hemC"
                     /locus_tag="PL152_00220"
     CDS             4809..5750
                     /gene="hemC"
                     /locus_tag="PL152_00220"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004418 - hydroxymethylbilane synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HQR47723.1"
                     /translation="MSNPIRIATRRSKLALWQAEHVAALLRGAHPQVPVELVPMSTQG
                     DRVLDRALADVGGKGLFVKELEVAMQEGRADIAVHSMKDVPSELPPGFAIVAALPRAN
                     PNDAFLSLRYARFAELPRGARVGTSSPRRQAQLRHARPDLALELLRGNVDTRLRRLEE
                     GSLDAILLACAGLERLGLGARITEVLDADFCVPAVGQGVIGIECRADDARSRGLLAAL
                     HHEESFVRLTAERAFARALGGSCHSPIAAHAALDGGVITLRGFVGAPDGTEVYRDRIT
                     GPAANADRLGLELARRVQAAGAGPLLERLRDEATATK"
     gene            5747..6598
                     /locus_tag="PL152_00225"
     CDS             5747..6598
                     /locus_tag="PL152_00225"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: protein motif:HMM:NF014641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006780 - uroporphyrinogen III biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006782 - protoporphyrinogen IX
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HQR47724.1"
                     /translation="MTAPLAGLVVVVTRPARQAARMLEHLRQQGAATIAFPTILVEPV
                     ALDAAARAALAPGRFDWIVYTSANSVEMSVPQVGRPGDATAVAAGDAAPARTEGASRT
                     SPPRVAAIGRATARALADAGVHVDVIPESGADSEALLAHPALAQPRGLRFMLVKGVGG
                     RDALRAGLEARGAIVTMAEVYRRTRATPSPLALDALHASREQGRFVVAVTSVEILESL
                     LALVPETIAPWLRDAPLLLPGERVAAEALRRGWRGPLIVARSAEDETMVETLRDWVVH
                     GLGSGRR"
     gene            6654..7697
                     /locus_tag="PL152_00230"
     CDS             6654..7697
                     /locus_tag="PL152_00230"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF016275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HQR47725.1"
                     /translation="MSAEIESLSSDMSSRRTGGGTSPMLVAVSVLALLLAGWAHWRFN
                     HFDDRVDKVRRQVAELRESRNRLAAQISALTTRLETSQNAMRAELGALKELPAQLAVL
                     GRGVEELKARTEAPQRAWARAEALYLLDLAQRQLDLDHDIGTAIAAMEAADARLAAFN
                     DPAMGEVRRLLAQDLGALRAVTVPDLPAVLERIAAVEGAAATLPVLGVPVAREATGAG
                     EPAGTSAFRRALRRIADALSGLVSFRRVDPAAMQLVTQEEQSLRRQHLELLLLAARVA
                     AMQPDSSAYTSSLDAASKWLDQYFDGSSPAVVAARKEVAALRNVSVEPPVPKIGAAAR
                     QLQLVMHGGTSAP"
     gene            7694..8902
                     /locus_tag="PL152_00235"
     CDS             7694..8902
                     /locus_tag="PL152_00235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HQR47726.1"
                     /translation="MRGAVIVALALVGGGLVAQLLLTDPGYVAVRAGRSLVETTLPVL
                     LAFLAAAYLVVRGIAGIISARRRLAELHAERRRRRARDDTRRGLLDLAAGNWRRAEEL
                     LTRSAADADSPASNYLIAARAADLLDAIDRRDQWLTQAQESAPEERAAALVTLAEMHL
                     RRGQDAAAVRTLEQLEASGDLNSRGLELLARLYPKLGQGEKLLALGPRLMAAKELPPA
                     QVSELLAQVQLEEIRAAGAHNDQARLEKAWSDLSRGVRRLPQSIVTYARAAMGCNDHP
                     TAEKVLRELIDDTSDPAAVRLYGDLVLADPLAPLDRAEGWLRKRPEDPELLATCARLC
                     LRAELVGKARSYLEASLARRPSSENSLILADLLDQIGERDRAADCLRKAVTLSVGRRP
                     NVPRVRLRRR"
     gene            complement(8910..9953)
                     /locus_tag="PL152_00240"
     CDS             complement(8910..9953)
                     /locus_tag="PL152_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQR47727.1"
                     /translation="MPQVTLVPSGQDFTAERDEPVLAAALRAGLNLPHSCKGGHCSSC
                     LARIVSGTIAYHGERPVGITEDEARRGYALLCQARALTDLRVEAREMRPAPEVDVRSL
                     PCRIERKVLLAPDVMAVFLRLPAVEALHHRAGQYLDIMLSEGRRRSFSIASAPADGPL
                     VELHVRRASTSGFTAQLFDVLRPGALLRIEGPLGQFWLRAESPRPTLMVGGGTGYAPL
                     RAMLRQMIAIGDRRPVTLYWGSRDLDGLYEHAWLRDLERSRPGFRYCPVLSEPQPIAA
                     SVRSGPVHEAVLADHPDLSAFEVYASGPPSMIETIREQFPAAGLPREQLHFDSFDYAP
                     DTMAKMSASTATS"
     gene            10037..10921
                     /locus_tag="PL152_00245"
     CDS             10037..10921
                     /locus_tag="PL152_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HQR47728.1"
                     /translation="MDRRCVIAGCGYVGRRLAVSVQRSWQVVAVTRAPATVADLRAAG
                     IETIGVDFDSDQAAPELRSLADGAAVAYLAPPPNHGTTDPRLERFLGLLADARPAVLL
                     YMSTTGVYGDAAGAAVDESSPAAPTNDRSRRRLAAERVAQSWSGARGTRCVILRVPGI
                     YGPHRLPLERLQRGEPVLRAEDSGPGNRIHVDDLVACCIAAIERPVDGVFNVGDGDHA
                     STTAFLQKVATLAGLQSPRLVSLAEARGQISPGMLEYLVESRRVLTTRMREELGVIPR
                     YGILESGIAASLAEMRIR"
     gene            10945..11787
                     /gene="dapF"
                     /locus_tag="PL152_00250"
     CDS             10945..11787
                     /gene="dapF"
                     /locus_tag="PL152_00250"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HQR47729.1"
                     /translation="MRLAFTKMHGLGNDFIVFDARAPGEVPSPAELRRLAARRTGIGF
                     DQALVLEPPRRDGTDVYYRIFNADGSEVEQCGNGARCIARLVATRASRQDRPLRLDSP
                     GGVVEARLRRDGLVSVAMGVPDFSPASLPFVADREEPSYHIELGSGPIEFGAVSIGNP
                     HAVIRVRSVTDAPVDTVGPAMENHPRFPRRVNVGFLEIVAPDHVRLRVFERGAGETRA
                     CGTGACAAVAVARKHGPLAEEVRVDLPGGRLVVQWAGPGEQVWLTGPAETAFEGQVDV
                     ADAP"
     gene            11801..12514
                     /locus_tag="PL152_00255"
     CDS             11801..12514
                     /locus_tag="PL152_00255"
                     /inference="COORDINATES: protein motif:HMM:NF016243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 family protein"
                     /protein_id="HQR47730.1"
                     /translation="MNKQPIRPTGPEAIAEEQVGDYLVAHPEFFERHPGVLARLRLPH
                     QRGSAAVSLVERQVLVLREKHGALEQKLQELIENGRANDAIADRMHRLTRRLLRARDL
                     GSTLAGLETSLREDFGASRWLVLLTDSALPGLAGHQHPHVRVVPRGSPELRIFETFFE
                     SARPRSGQIRDTQRDYLFGTEGGPVGSTVLIPLGERASLGLLAIASHDTERYLPTMST
                     DFLVRIGDIVTEAITSRAA"
     gene            12511..13416
                     /gene="xerC"
                     /locus_tag="PL152_00260"
     CDS             12511..13416
                     /gene="xerC"
                     /locus_tag="PL152_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HQR47731.1"
                     /translation="MSEALAGGVTRFLQGLRNERRLSPHTESAYARDLESLMEYCASQ
                     GIASWDQLDPQHVRSFAAQCHRRGLSPRSVQRRLSAARSLFRYLMREGELSRDPATDV
                     QAPKARKRLPVTLDADAMARLLEFRTDDRLGVRDKAIMELFYSSGLRLSELLGLDLTD
                     LDLRDRTVRVLGKGRKARIVPVGRHAAEALSRWLRERTTLADVGETAVFVGTNGRRLG
                     PRIVQRRIARWARLQGLPEHVHPHMFRHSFASHLLESSGDLRAVQELLGHANISTTQV
                     YTHLDFQHLARVYDSAHPRAKRRRA"
     gene            13459..14013
                     /gene="hslV"
                     /locus_tag="PL152_00265"
     CDS             13459..14013
                     /gene="hslV"
                     /locus_tag="PL152_00265"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000072681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HQR47732.1"
                     /translation="MTSFDPHGTTILCVRRHGLVAMGGDGQVTLGNTVMKGNARKVRR
                     LHEGQVVAGFAGGTADAFTLFERFEGKLQQYGNLTRAAIELAKDWRSDRSLRRLEALL
                     CVADASHSFIVSGNGDVIEPEHDLMAVGSGGAFAQAAATALLRNTDLDARAIVEQALG
                     IAADICIYTNRNLTIETLGKPSAG"
     gene            complement(14079..14693)
                     /locus_tag="PL152_00270"
     CDS             complement(14079..14693)
                     /locus_tag="PL152_00270"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQR47733.1"
                     /translation="MTRAWRGSRFARCIAALTAALGCAPAEAHLNSTGMGPVYDGLAH
                     FAMSPDDLVPVLAVALLAGLNGADAARRALFALPAAWLAGCLLGMDAPVVNPGSAWAA
                     LNFLVLGGLVVADAKLSPRVLTALAVVLGLAHGYPNGSGLGRSGQSFVAMLGLTSAVF
                     VTIALVSAFVVRLRAHWARIAVRVAGSWIAASGLLMLGWAMRGT"
     gene            complement(14690..15250)
                     /locus_tag="PL152_00275"
     CDS             complement(14690..15250)
                     /locus_tag="PL152_00275"
                     /inference="COORDINATES: protein motif:HMM:NF016819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQR47734.1"
                     /translation="MAIACWTPAALAHVTKGQAYGLLTGFMHPLSGTDHVLAMVAVGL
                     WGAQLGAPAIWVLPVAFPLVMAMGGMLGFLGVPLPGIEYGIAASAIVLGAAVAFEVRP
                     PLVVAALLVGIFAIFHGYAHGTELPPGQSAMLYSLGFVVATGCLHAVGIGIGTAHRWP
                     WGRRLLRGAGAVVMAGGVYFLWRAIA"
     gene            complement(15292..16317)
                     /locus_tag="PL152_00280"
     CDS             complement(15292..16317)
                     /locus_tag="PL152_00280"
                     /inference="COORDINATES: protein motif:HMM:NF025167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HupE/UreJ family protein"
                     /protein_id="HQR47735.1"
                     /translation="MQSSLRHVAVRIVAMLVAALGCVPPAQAHESRPAYLEVRETAPG
                     QYSLLWRTPMLAGMRLPVVLQLPQDVRNVRSPEIDQLADSVVERRWIDAGKGGLAGRR
                     IDFPGLQLTITDVLVRVERLDGTTTTAIARPSHPWVEIAASQTRWQVMGAYVVEGMRH
                     ILFGADHLLFVLGLLLIVRDRWMLVKTVTAFTVAHSITLAIATLGYAQAPALPLNAAI
                     ALSILFLGPEIVRVWRGETSFTIRHPWVVAFAFGLLHGFGFASALTSAGLPQQDVPIA
                     LFSFNVGVEIGQLTFVGLVLALERSFRILRVRWPRWVQALPGYTVGALGAFWTIQRVA
                     VMFGAMR"
     gene            complement(16326..17243)
                     /locus_tag="PL152_00285"
     CDS             complement(16326..17243)
                     /locus_tag="PL152_00285"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR47736.1"
                     /translation="MNRIRRFLREPLVHFLLLGAALFGLYRYYGPTTDAGPSSKQIRF
                     TLDDLGQMIAVFRSQWQREPTPQELSLLVEEKVQQEILYREAIAMGLDQDDEIVKRRM
                     AQKMQFLAEDVATSREPTTDELQAWFAKNSQRFAMPPRISFRHLYFSPDRRGTRARDD
                     AARALEQLAGKPEDAPLASTLADPFMFQDYYRDRAPDYLGKEFGPQFAQAVEKLPAGS
                     WQGPVESGFGWHLVYVDTVIPGRVPDFEEVEPDVKTAWLAERKAEAWAKAYKDMREKY
                     TVLLPVPAAVAADIPVPQRPAAAPQPTAD"
     gene            17456..18781
                     /gene="hslU"
                     /locus_tag="PL152_00290"
     CDS             17456..18781
                     /gene="hslU"
                     /locus_tag="PL152_00290"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HQR47737.1"
                     /translation="MTPREIVQELDKHIVGQGAAKRAVAIALRNRWRRMQVPERLRAE
                     ITPKNILMIGPTGVGKTEIARRLARLANAPFVKVEATKFTEVGYVGREVDSIVKDLVD
                     VAVKMTRESETDKVRHRAMDAAEERVLDVLLPKPRNVGFGSEASADRDGDTRQRFRKM
                     LREGQLDDREIEIELRAVPMGVEIMAPPGMEELSQQLQGMFSSLGGDRTRPRKVKVRE
                     AFKLLTEEEAAKLVNEEELKVQALRNAEQNGIVFIDEIDKIARRQETVGADVSREGVQ
                     RDLLPLVEGCTVNTKYGMVRTDHVLFIASGAFHMSKPSDLIPELQGRLPIRVELEALG
                     VDDFVRILTEPDASLCRQYSALLETEGVRVEFAADGVRRLAEVAFDVNQRTENIGARR
                     LHTVMERLLEGLSFEASDRSGASVVIDASYVDRNLGELARDQDLARYIL"
     gene            18788..19177
                     /locus_tag="PL152_00295"
     CDS             18788..19177
                     /locus_tag="PL152_00295"
                     /inference="COORDINATES: protein motif:HMM:NF017922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF971 domain-containing protein"
                     /protein_id="HQR47738.1"
                     /translation="MADPRPTDIKLRTRSRVLEITFDDGERHELPFEYLRVYSPSAEV
                     RGHGPGQETLQVGKHEVGVKAVETVGNYALRIVFDDGHDTGLYTWGYLQELGRTRAEK
                     WQRYLDRLAKLGLPYPTPVPTRLPRTQ"
     gene            19198..19704
                     /locus_tag="PL152_00300"
     CDS             19198..19704
                     /locus_tag="PL152_00300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47739.1"
                     /translation="MARPVSPMIPGIKALVLWLGLTAAAVVVADDLRQAHLFLRSNHI
                     YIRIPDGPEYRVEVDSPVYEEYAREHGADCETPPEVREKVEIRQFYKPSKGGVPGRVR
                     VELLVGPAGRVVAIQALESGAGGLSLYSVLYATRRWIFEPGRDRSGQAAFCRYPVSVE
                     FVLPDTQG"
     gene            19762..20532
                     /gene="ubiE"
                     /locus_tag="PL152_00305"
     CDS             19762..20532
                     /gene="ubiE"
                     /locus_tag="PL152_00305"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HQR47740.1"
                     /translation="MSEPSDKQAASGSVDFGYKQVPKDEKAKHVRAVFDSVADKYDLM
                     NDLMSAGVHRLWKRFALSQTGLRPGQAALDVAGGTGDLAAGMARQVGDSGLVVLSDIN
                     AAMLEVGRGRLMDRGLMRNVRFSLANAECLPFEDESFDCVTIGFGLRNVTDKPAALAS
                     MRRVLKPGGRLLVLEFSKPVVPGLKPIYDTYSFRVLPWLGRRVAGDADSYQYLAESIR
                     RFPDQETLRTMMGDAGLEDCSYHNLSGGIVALHKGFRY"
     gene            20534..21169
                     /locus_tag="PL152_00310"
     CDS             20534..21169
                     /locus_tag="PL152_00310"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQR47741.1"
                     /translation="MLLEPLGDLLNRNIADSRRAQALCRQLDGRTVSLVVEGTPLAFF
                     FRSEGGRVALTASHDGATDATLSGTPIGLLALAGPRAEGALRGGGVRIEGDAEVAQKF
                     RDLLAESQPDFEEELARVIGDVAARNIANLARGFLDFGRKAGRSLAGNVSEYLQEEGR
                     DVPTRTEMEEFLSDVDHLRDDVERLDARLARLESGAGKPASPQGATRSKAQ"
     gene            21236..22873
                     /gene="ubiB"
                     /locus_tag="PL152_00315"
     CDS             21236..22873
                     /gene="ubiB"
                     /locus_tag="PL152_00315"
                     /inference="COORDINATES: protein motif:HMM:NF003404.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HQR47742.1"
                     /translation="MRFRVIGRLLQIQRVLVRHGLDEIILATHLFRPLRFFFYLSPAT
                     WFERRRGGTRGERIRLALEELGPIFVKFGQALSTRRDLLPADVADELEKLQDRVPPFP
                     GAEARAIVERAYGRSVTEVFEQFDESPLAAASVAQVHTARLRTGEEVVVKVIRPGVRE
                     KIERDLEVMYVLAGLARDYASEGHRLRPVEVVREYEKTILDELDLMREAANAAQLKRN
                     FAGNELLHVPAVYWDYCRPDAMVMERIHGVIISDMPELRRRGTNIQRLAESGVTIFFT
                     QVLHHNFFHADMHPGNIFVLLEDPEQPKYCAVDFGIMGTLTARDQHYLAENFLAFFER
                     DWRRVAQLHVDSGWVPAETRVDELETAVRTVCEPIFQKPLSEISFGQVLLRLFEVARR
                     FQMQVQPQLILLQKTLLQIEGLGRQLYPELDLWKTAQPILRDWAADRLSGRALAQDLR
                     RQLPDLSEALRMLPQVLQQVLQHASDGTFRLKVEQAGLEQIREEIRRGARQRDATVVA
                     AAAMLAGLVWLAIGMSAWPGIALCAGGLVGALVARRW"
     gene            complement(22903..23613)
                     /locus_tag="PL152_00320"
     CDS             complement(22903..23613)
                     /locus_tag="PL152_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47743.1"
                     /translation="MSTLSIGRRFNGPPGSANGGYACGALAVAANADLKVRLLAPPPL
                     EEAMALERKDDGSWTLASQAGPVAVGTPGRLVLDVPSPPAYVQAVWASQHYPGFHQHA
                     FPDCFVCGPHRRRGDGLRIFPGMLDTGIVAAPWLPADDLDRGDGRVAAVFHWAALDCP
                     GYFAVSAGRRPMVLGEMQAHIDRRVHTGESCTVIGWKIGSEGRKHHAGTAIFDEDGEL
                     CARALATWIEVKLPVGGP"
     gene            complement(23610..23936)
                     /locus_tag="PL152_00325"
     CDS             complement(23610..23936)
                     /locus_tag="PL152_00325"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HQR47744.1"
                     /translation="MRKVHTAESTIEIAHLRNVLESAGIPCVVRNDRLSGVIGEIPFV
                     ECWPELWVREPGHALRARGLIDEALRPAQDSEGWTCARCGERCEGQFTECWNCAARAD
                     GGTGEP"
     gene            complement(23953..24636)
                     /locus_tag="PL152_00330"
     CDS             complement(23953..24636)
                     /locus_tag="PL152_00330"
                     /inference="COORDINATES: protein motif:HMM:NF014600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HQR47745.1"
                     /translation="MNELELTGRARTHVVDIEHPRCVLHVEAVTSFLAMRDAAAAEGL
                     DLAAASSFRDFERQVHLWNRKWRGERPLQDRAGKPIDPSTLDDARKVDAILCWSAIPG
                     GSRHHWGTDVDVIDAAAMPAGYQVQLVPAEYAGDGVFGRLSAWLDSNMTRFGFYRPYA
                     SVGSGAGVEPWHLSYWPIATEALEALTLPVLRAAVAGSDMLGKAQVLERLPEIYTRFI
                     LAVDRPRKA"
     gene            24897..27017
                     /locus_tag="PL152_00335"
     CDS             24897..27017
                     /locus_tag="PL152_00335"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR47746.1"
                     /translation="MLTVVAVGVLLTAAVSAMVLETASADAQRVRNVLAQAQEVAAAD
                     PTAALAIVTDDAMHSIQRIVWRRLPPVIASGLLLTLLAGFVTWRMVLRIDRGVEALMH
                     SAEQIGSGHQAEPVTATGVREISAIEESLERMRQALAQTTISRDYLGIVLNSMSDAVL
                     VTSTDGRVRTANRAATQLLGLGLDRLVGVDLVSLVAEDHRATFDPAKVLAAPGETVLS
                     TARGQTIPVSVSASAISSDDPRFKGSIFVLRDITERKRAERRIRYLARFDTLTKMPNR
                     MQFQHLLHQAITRATRQGRSLALLYVDVDNFKDVNDTFGHDAGDRVLETLAERLSRIL
                     PKESVVGRLAGDEFAVFAETPDGEQDRHEQAASLARMLLAEIARAQHVGEHEIEVTAS
                     IGIAACPDDGDNVIDLIRNADAAMYHAKRAGRNCHVFYAPEMNAAAVERLLLKGKLRR
                     ALGRNEFVLRYQPKVDLASGHVVGAEALLRWRLPGHGEIAPAQFIPLAEESLLIRDIG
                     AWVLNQVCSDYAVWQKRVSDPGIVALNLSLKELSHASFIPRCRSVFQQHGVSPGRVEL
                     EITETTLMMDAERTLPLLDELRAMGVHLAIDDFGTGYSSLSALQQFPIGTLKIDQSFV
                     RNAATDPDDATIVRTIIEMGRSLGLQVLAEGIETEEQRAFLLHSGCEYGQGRLFGEPM
                     SADEFLETMVRQATGVHPVLRLPA"
     gene            complement(27084..27698)
                     /locus_tag="PL152_00340"
     CDS             complement(27084..27698)
                     /locus_tag="PL152_00340"
                     /inference="COORDINATES: protein motif:HMM:NF015823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HQR47747.1"
                     /translation="MRSLGPVLLLASLCTACVGTALRSNEAPPEQYRLEAPAAVPGGP
                     VLQLAIAVARPRAPSSLDTDRIAVTTPGNGFDYLAGARWADPAPQMLQQLLVVTLAGD
                     GHFATAVAAPSRVPPDLLLDVELRRFEAAYAPVDAPPTVVVEFEANLVDARKGLRMAS
                     FRSAASVPAASNDRTAVVAAFEQAAGQAVRDAATRVAAAAQGTP"
     gene            complement(27695..28630)
                     /locus_tag="PL152_00345"
     CDS             complement(27695..28630)
                     /locus_tag="PL152_00345"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HQR47748.1"
                     /translation="MERDAKYAAVAAFALLAVAATVVFVLWYSGHGDQRQYVRYEIYF
                     DGSVSGLSRGSPVRYLGVDVGRVTRLSLDRENPGRVKAVVEVDAQAPVSGATQARLGL
                     LGLTGLLYIDLQKNPAADPSRPLAKGTEFPVIPSRPGDIEAFLAKLPDLLGHVAGVTE
                     RLELLLADRNLDAIAQSLQNVKVASRDLPAVTHEAAALAGELRNAATEVTALTASLRE
                     AAGTAGPKLEATLTGMNNAVDKLGRTADSLDRIVNGNEATLTQFAGSGVADLQQLIGD
                     LRETSEEMRELARSLRDNPSSLIIQRKESGMEIPR"
     gene            complement(28620..29396)
                     /locus_tag="PL152_00350"
     CDS             complement(28620..29396)
                     /locus_tag="PL152_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR47749.1"
                     /translation="MQTVVSARDIVNRFGAQVVHDGVSFDVHHGEVFGIVGGSGSGKS
                     VLLKTLLGLRHPDSGTVEIEGREITSMGDAELREIKRHYGVTFQQGALFSSLTVAENV
                     ALPMHEAMTLAPDTVASLVELRLRMVGLPLDAAVKYPAQLSGGMVKRVAVARALALDP
                     KLLFLDEPTAGLDPIAAAAFDALVLYLRKALGLTVVMVTHDLDTLARTCDRVAVLVDG
                     KVIVDTLEGIVANDHPWIRQYFHGARGRAAVPQQGADGGT"
     gene            complement(29405..30520)
                     /locus_tag="PL152_00355"
     CDS             complement(29405..30520)
                     /locus_tag="PL152_00355"
                     /inference="COORDINATES: protein motif:HMM:TIGR00056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR47750.1"
                     /translation="MTIQALLGEARDRLSITGDCGIADVPELASRLDAIREAAPARLT
                     IDFTRAGAFDIGPAWLLHEAISGLRARGAEVTLEGTPPVHFAYLDGLAASDAGAPPAA
                     PETGLLPWLAGRGRDLEVRATGWYAALTFGGEVIVTAGSRWTRLKGLRVASVVRHTYE
                     TGVRAIPVVAMIAFLISVIAAYISAQQLRPFGAEIYTVDVVAVGVLRELGVLLTAIMV
                     AGRSGSAYAAELGVMKLNDEVDALESMGLNPLELLVLPRVLGLVIAMPLLTIVADAMG
                     LVGGAMLTRYLFDLSWPQFVARLDDAIADTTFWAGLIKAPVFGLVIALTGTLRGLQVR
                     ESSRELGRLTTVAVVQSIFLVILVDALFAVLFLEIDF"
     gene            30581..32560
                     /locus_tag="PL152_00360"
     CDS             30581..32560
                     /locus_tag="PL152_00360"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HQR47751.1"
                     /translation="MTGAVLSQILILLAGSVIALSLVRRFSLPPILGYLLVGLLLGPS
                     ALHLAVDSASVALLAEFGVVFLVFTLGLEFSFARMVAMKSEVLGVGGLQMLITTAVVG
                     SVAWAFGLAPAVALVVGGALAMSSTAIILRQLGDQIELNRTHSRLALGILLFQDLAFA
                     PLLALATSLGGSGEMPGGAWLLGMAARAVAALVAVLALGRWVLRPLFHEIARHRSTES
                     FTLTVLFVALGGAWSTHALGLSMALGAFLAGMLLSETEFRHQTEAVIKPFQAILLGLF
                     LVSVGMLLDLQLLLTRLPLILLVTVALLVVKAAIVALVVRGFVPNTRKALRTGIVVSM
                     GGEFGFALLTLLLKSHMADPVIVQPLLTAVALSLLIGPLLVRYNGRIADRILRRPTVE
                     ASEVARETVATRQLATREHVIICGFGRVGQNLARVLEARGLEYIALDLDPFRVRSARQ
                     AGDPVVYGDASEAEVLRALGVEHASVLVVSFEDPQRALGIVRTARQLRADLPVLVRTG
                     DDAKLEELQTAGATEVIPGTLETSLALVAHVLLILKVPAQEVMQTTENIRHDRYSILR
                     SVFRKRRARSLGGDHALRQQLRTVVLPPGAQAVGHTIRDAGLDEGSVRVRAVRRDGIV
                     GRDPDGDTRLREGDVLVLWGPPEELDVAENRLLMG"
     assembly_gap    32859..32958
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32959..33549
                     /locus_tag="PL152_00365"
     CDS             <32959..33549
                     /locus_tag="PL152_00365"
                     /inference="COORDINATES: protein motif:HMM:NF024701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR47752.1"
                     /translation="DAADAVYAESGWDSVTMDQVARRARLSRALVYVYFKDKPDLHLA
                     LVERSLDTLRLRFEAARAGKANGLGELEAIGRAYVAFSRELPHYYDACARYQAHDCGP
                     ACDGGDLPANEAACMAAGRRVHETIVESIRRGIADGSVRADIGDPMPTALALWAFTHG
                     VIQIAASKGGQIERDGVPIQQFIDHAFSLALRSLRP"
     gene            33566..35161
                     /locus_tag="PL152_00370"
     CDS             33566..35161
                     /locus_tag="PL152_00370"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR47753.1"
                     /translation="MRRSPAVLKSRRPSEIGVALPLLTWALMAGTTTPGIARAETTLP
                     AVPSARQVAALSLASAAGTQIQDPRPVTSAGTTVEVGLREVLGEALAANLELRAGAAG
                     VAQRVAALDRARARYLPVLDFDARYSVADGGRTIDFPVGDLLNPVYETLDQLLVQSGQ
                     PAAFPRVKNEEIRLLLPREQNTRLVLAQPVYEPRLGPAVEGTRQELNRVEADYSGLRS
                     RVIRDTKQAYYEWLVAQQQVLVFDATLELARSNLAATESLYRNGRITRDLVYRAEADL
                     LEIEQQRLAAASRVTIAQSYVNLLRNQPLTSPVPAATIDAATVERFRDRLVRRAAGRR
                     LDAPALEEVATGQRAEIQGLDAAIAGSLAQQDLARAAFKPAVAVGAEAGIQGEDYGFG
                     PDERYVLASVVLRWNTFRGGADLAALAEARALTDELRATRDLATQQVRLQVQRAVEQL
                     GVAEASLATAEKRAVAAEAAFRITERKRDLGQVNQTDYIDARKTLTDAELNVNRVRTE
                     FLARIAELEFAIGDRRAADEEPQ"
     gene            35158..>35827
                     /locus_tag="PL152_00375"
     CDS             35158..>35827
                     /locus_tag="PL152_00375"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR47754.1"
                     /translation="MNRIAVAVLTTAALAVAACSRETPAPEPAHASATPVRAAPVETV
                     QLAGSVRAVGTLEPESEVRLSFKVGGVVDSITVHAGDQVRRGQLLATIKRAEVDTALA
                     QAAEAEAKAQRDLDRARRLREDEVATEEQVQDLATAWKVARANLDAARFNARYSRIEA
                     PQDGVVLSRLAEQDELVQAGQPVLVLGGTGGGWIVRTALADRDAVRVEVGDTARVEFD
                     AFPGR"
     assembly_gap    35828..35927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            36092..>39120
                     /locus_tag="PL152_00380"
     CDS             36092..>39120
                     /locus_tag="PL152_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR47755.1"
                     /translation="MRIWAFSVRRWQFTLLLFALLTAMGVSSFLGIPRSEDPEFHVPI
                     PTIVVVYPGADPVDIERLVVDPIEDALSELDDVKRIDSASMDGVGVIRPEFHWDTDAD
                     DKYDEVVREVSRIRGELPAGITSLEIRKAGSGLVNIVQVALVGAQVPDRELKKLAEDL
                     SDSIETVPGVRSSAWWAVPEPEVRVSVDLERMGRAGVTLTQVEQAIRGGNATVPGGAV
                     DVGLRKYNLKTSGGFDSLPEISDTVVASRDGRVVRVADVAAVSWASAEETYVGRYKGE
                     RAAFVTANAKDAVNVFAVRNAIYAKLGAFEKELPAGVRLERGFDQTRNVAHRLKRLGM
                     DFAIAIALVLLTLLPLGLRASAVVMISIPLSLAIGLSLLYYTGFSLNQLSIAGFVLAL
                     GLLVDDSIVVVENIARFLREGYSRVEAAIAATDQISLAVLGCTATLLFAFLPLLFLPE
                     GAGKFIRSLPAAVLYTVLASLFVALTVIPFLASRMLRPEGHDGEGNRVLRGLMSVINR
                     VYAPALRVALAWPRRTLLAALAFVMASLAIVPFIGVSLFPPADIPQFRVTIETPDGAS
                     LADTDRALRFVEAELGRHPEVKQWFANLGHGNPRVYYNIFPEETKANTAEVFAELHHF
                     DPRRSPALYEEIRAKFARYPGARILLESYQNGPPINAPIEISVAGPDLGQLRELAARV
                     EKEIAGTPGTRDVDNPLRRQRTDIDLRIDPEKAALFGVAPVDADRTVRAAVAGLPVGS
                     YRESDGDEYDITLRLPMQGRQTLDALGLIEVASASGRPVPLRQISSPQFAAAPSTIER
                     HDRVREAKITAYPAAGYNTGKVTRAVMRRLDALGLPPGYSLRVGGELEAREESFGGMG
                     PAVLVAVFGILAVLVLEFGSFRSMLIVAGVIPLGVAGGLVALLLTGYTLSFTAMIGLI
                     ALIGIEIKNSILLVDFTNQLRAQGVEVDEAITRAGQIRFLPILLTSATAIGGLLPLAV
                     QGSGLYSPLAIVIIGGLVSSTLIGRLVTPVMYKVLPPA"
BASE COUNT         5796 a        13789 c        13613 g         5722 t
ORIGIN      
        1 cgctttcggg cacggaaccg gcgaggcgcc gggccaggtc gtcgatgagg cgagggtcga
       61 agggcatggc agggggctgg ttgggctgcg ggagacgtag tgtaaggaag aacgccgggc
      121 gggatcagcg cctgcccttc gggcagcagc cggcgcggcc gcggccgatg cgctctgcgg
      181 tgaggcggcc gtccccaccg tacgccccac gatggtgcag gcgttcattc cgcagccgga
      241 cgcgctggcc aaaaagaggc ggccgggcag gaccggggtc gtggcacgct tcctgcattg
      301 aggtccccgg gttccatgcc attcccggag gtctgcccgc gatgaagctg atcaccgcca
      361 tcatcaagcc cttcaagctc gatgaagtgc ggcaggccat cgccgacatc ggcctgcagg
      421 gcgtgacggt caccgaggtg ctcggcttcg gccgccagcg cggccgcacc gagttctacc
      481 ggggcgccga gtaccacagc ggcctgctgc cgaagaccaa ggtggagatc gcggtcgagg
      541 acacggtcgt cgacccggtg gtcgaggcga tcagcaacgt cgcgcgcacc ggccgcaccg
      601 gtgacgggaa gatctgcgtg accaacttga ggcaggccgt gcgcatacgc acgggcgaac
      661 gcggggccga ggccgtctga aacggacgat gccgcgcaca gacattcaat ccgacgaccg
      721 accgacagga gaacgccagt gaacacgttg accgccgcgc ggccgggact cagggcagcc
      781 gcactcgccc tgggcaccct gcttgccccc gccctcgcct tcgccgccga ccccgtgccg
      841 gacaagggcg acacggcgtg gatgctcacc tcgaccatgc tcgtcatcct gatgacgatc
      901 ccgggactcg cgctgttcta cggcggcatg gtccgctcca agaacgtgct gtcgctgctc
      961 atgcaggtgt tcatgacgtt ctgcctggtc gcgatcctgt gggccgtgta tggctacagc
     1021 gtcgcgttca cgtcgggcaa cgcgttcttc ggcggattcg accgcgcgtt cctgcagggc
     1081 ctcacgtcga gttccgtcgc ggcgaccttc agcaagggcg tctacatccc ggagtacgtg
     1141 tacttcgtgt tccagatgac gttcgcctgc atcacgccgt gcctgatcgt cggcgccttc
     1201 gccgagcgca tcaagttcag cgccgtgctg ctgttcatgg tgctgtggtt caccttcgcg
     1261 tacctgccga tcgcgcacat ggtgtggttc tgggccgggc cagacgctta taccgaccca
     1321 acggccgggg ccgccgccac cgctggcgcc ggcttcctgt tccagaaggg cgcgctcgac
     1381 ttcgcgggcg gcaccgtcgt gcacatcaac gccggcatcg cgggcctggt gggcgcgctc
     1441 atgatcggca agcgcatcgg ctacggcagc gccgccatgg cgccgcacaa cgtgccgatg
     1501 gtcatgatcg ggtcgtcgct gctgtgggtc ggctggttcg gcttcaacgt gggatcgaac
     1561 ctcgaggcca ccggtttcgc gggccaggtg ttcgccaaca cgctgctcgc gacggccgcg
     1621 gccgcgctcg cctggtcgtt cgccgagtgg atccaccgcg gcacgccgac gatgctgggc
     1681 gccgcgtccg gtgcggtcgc gggtctcgtc gcgatcacgc cggcctgcgg ctgggtcggg
     1741 ccgatgggct cgatcgcgat cggtctgatc gccggtctcg tgtgcctctg ggccgtggtg
     1801 agcctcaagc agaagctcgg ctacgacgac tcgctcgacg tgttcggcgt gcactgcgtc
     1861 ggcggcatcc tgggcgcgct gctgacggcg gtgttctgct cgcccgcgct cggcggcacc
     1921 ggcgtgtacg actacgtcgc gaatgccgtc ggcgactact cgatcgcggc ccagttcaag
     1981 tcgcagctgt gggcggtgct ggtgtcgctc gcctggtcgg gactggtggc tgcgatctcg
     2041 ttcaaggtcg tcgacctggt cgtgggactg cgggtcgccg aggaccagga gcgcgagggc
     2101 ctcgacaccg tgtcgcacgg cgagcgggcc tataactgac cccccggggg ggcactgcgc
     2161 agaccccatc ggggcgggta ccccggtggg gtctgcgtct cagtttggcc gggccgggcc
     2221 gcggctccgg aggacatttc cggcggtcct gctagactcg ggacatgctt cggagccccc
     2281 atatgcgacc gatcgcgaca ctcatggcag gcctggtcct ctccgccgcg gcggcggcag
     2341 gggacgtgta cgtgaccaag gatgcgcagg gcaacctgat ctacacggac acgccgctga
     2401 cggtcccggc gaagaaagtg gacatccaca gcgacagcac caacccggag acggcggagc
     2461 agcgctatac gtcggagatg tcgcagtacg ccaaggacaa ccaggcggcg gccaagtcgc
     2521 agtcccagca ggccgcccag cagcaggcgg cccaggacac cgctgcggac caggccaagc
     2581 gctgcgtgga agcgcgccag acctaccaga cctacatgaa ctcctggcgg ctgtacgaga
     2641 cgggtccgaa cggcgagcgc acgtacctga ccagcgagca gatcgacaag gcgcgcgccg
     2701 acgccaagca ggcgatggac cagttctgct ccgggcagta gtcggggcat ggtgaccgcc
     2761 gctggcttcg tcggcctcgg cgcgatgggc cggggcatgg cgcgcaacct gcaccgggcc
     2821 ggcctgctcg gcagcgtctg gaatcgcacc cacccggtcg cggagcaact cgcctctgaa
     2881 ctcggtgtga cggtcgcggc gagccccgcg gccctggctg cggcgtccga cgtcgtcgtg
     2941 ctgtgcgtgt cggccgacgc ggacgtcctc gccgtggtcg atgcgatgct gccgtcgatc
     3001 cgccccggca cgatcgtgat cgactgctcg acggtgagtt cggacaccgc gcgcgccgcg
     3061 gcggcgaagc tcgcggggtg cggagcggat ttcctcgacg cgccggtcag cggcggcgtg
     3121 gaaggcgcgc gcgacgggac gctcgcgatc atggtcggcg gcagtcccga agcattcgag
     3181 cgcgccgcac cggtcctcca ggccatgggc cgcaccgtga cgcacttcgg cccgagcggc
     3241 gcgggccagg ccgccaaggc caccaaccag atcatgtgcg caggcatcat ccaggcggtc
     3301 gccgaggcca tggcctttgc gaaggccgag ggcttgccgc tcgcgcagct cgtcgagacg
     3361 ctcggcaagg gcgccggctc gagctggtac ttcgtcaacc gcgcaccgaa catggtgcgc
     3421 ggcacctacc ccgcgggctt ccgcgtgcgg ctgcacgaca aggacctgcg catctgccgc
     3481 gacatggccg cgcgccacgg cgtcgagctt tcggtcgtcg agtccgcgct cggccactac
     3541 cgcaggttga tcgagcaggg ccacggcgac gaggacatct cgacgatctt ccgcctcacc
     3601 gacgcgctgt tcacgcacga gggcgcgggc gacgtcagcc cgaaaccgcg atgagcgagg
     3661 tgccggccgc acccgcaggc cgcagcgact acgcccgcat cggcggcctg ctgctcgccg
     3721 cactgctcgc atacctggtg tggagcgtcg tcgagccgtt gtggagaccg ctcgtgtggg
     3781 cggcgctgct cggctcgctg ctggccccgc tcaatatgcg gctcgcggtc cgcctcggtg
     3841 gccgcaagcg cctggccagc gtcgtcacca cgctcgccac ggtcctgctg ttcctcgtgc
     3901 ccgtcgggat catcgccggc gcggtcacga cgcaggccgc gcagctgctc gggcgcctca
     3961 attcgggcgc tgcggcgctg aacccgcagt cgttcgacat cgggcacgtg ccctggctgg
     4021 cgcggccgct cgagtggctg agcgagcaca cctcgatcag cgcggagcag gtgcagggct
     4081 ggaccgtcgg cgccgcgaaa cacgtgctgc agtcgatcgc gtcgtccggc ggcaatctcg
     4141 tgctcggcgc gctcggcacc gtcctgagct tcgcgctcat gctgttcgtg ctgttcttcg
     4201 tgctgcgcga cggcccggac gtcgcgcgca aggtggtggg catgctgccg atcgaggagc
     4261 gccgccgctc gcggctctgg cagcacctcg tcgaggtgaa tcgcgccgtg ttcatgggca
     4321 tcgggctcac cgccgtggtg cagggcgtgc tggtcggcat cggcttctgg atcgcgggac
     4381 tgccgagtcc cctgctgttc ggcgtgctcg gggcgttctg cgccctcgta ccgctgatcg
     4441 gaacggcgct cgtgtgggta cccggggccg catttctcgc gctgcacggc gactacggcc
     4501 acgccgggtt cctcacggca tggagcctgg tggtcgtcgg gtccgtggac aatttcctgc
     4561 gcccgatgct gatctcgggc cgcgccgaac tgcccacgct ggcggtgttc gtcggcgtga
     4621 tgggtgggct gtcggcgttc ggtttcatcg gcctgttcat cgggccgatc gtgctcgggc
     4681 tgctggtcgc cctgttccgc ttcgagcacg aggaacgcgc ggccgccgca aactgaagcg
     4741 agcgcgacaa gtctcgttcc gtgcacaatt ggcgacggga ccttccctgg accgcaccgg
     4801 agcccgactt gtccaatccg atccgcatcg ccactcgccg cagcaagctg gcgctctggc
     4861 aggccgaaca cgtcgcggcg ctgcttcgcg gcgcgcaccc ccaggttccc gtcgaactcg
     4921 tgccgatgag cacgcagggc gaccgcgtgc tcgatcgggc gctggccgat gtcggcggca
     4981 agggcctgtt cgtgaaggaa ctcgaggtgg cgatgcagga aggccgcgcc gacatcgcgg
     5041 tgcactccat gaaggacgtg ccgagcgagc tgccgccggg gttcgcgatc gtcgcggcac
     5101 tgccgcgcgc caaccccaac gatgccttcc tgtctctgcg ctacgcgcgt ttcgcggaac
     5161 tgccgcgcgg cgcccgcgtc ggcacctcga gcccgcgccg ccaggcccag ttgcggcacg
     5221 cacggcccga cctggcgctg gaactgctgc ggggcaacgt cgacacgcgg ctgcggcggc
     5281 tcgaggaagg cagcctcgat gcgatcctgc tcgcgtgcgc gggcctcgag cggctcgggc
     5341 tcggcgcgcg aatcaccgag gtgctggacg cggacttctg cgtgcccgcc gtcgggcagg
     5401 gagtgatcgg catcgagtgc cgtgccgacg acgcccgcag ccgcgggttg ctggcggcgc
     5461 tccatcacga ggaatcgttc gttcgcctca cggccgagcg ggcgttcgcg cgcgcgctcg
     5521 gcggcagctg ccactcgccg atcgcggcgc acgcggccct ggatggtggc gtcatcacgc
     5581 tccgcggatt cgtcggcgcg cccgatggca ccgaggtgta tcgcgaccgc atcaccggcc
     5641 cggccgcgaa cgccgaccgc ctcggtctcg aactcgcgcg gcgagtgcag gcggccggcg
     5701 cgggaccact gctcgagcgt ctgcgcgacg aggcgacggc caccaagtga cagcgccgct
     5761 cgccggactg gtcgtggtcg tgacccggcc cgcccggcag gcggcccgca tgctcgagca
     5821 cctgcggcaa cagggcgccg cgacgatcgc gttcccgacg atcctcgtgg aacccgtggc
     5881 gctggacgcg gccgcgcgtg ccgcgctcgc acccggccgg ttcgactgga tcgtctacac
     5941 gagtgcgaat tcggtcgaga tgtcggtccc gcaggtcggc cggcccggcg acgcgaccgc
     6001 ggtggctgct ggcgacgcgg cgcctgcgcg taccgaggga gcgtcacgaa cctcgccgcc
     6061 gcgagttgcg gcgatcggac gcgcaacggc gcgcgcgctc gccgacgccg gagtccacgt
     6121 cgacgtgata ccggagtccg gcgcggactc ggaagccctg ctcgctcacc cggcgctcgc
     6181 gcagccgcgc gggctccggt tcatgctcgt gaagggtgtc ggtggtcgcg acgccctgcg
     6241 tgcagggctc gaggcgcgcg gcgccatcgt cacgatggca gaagtctatc gccgcacgcg
     6301 ggcgacgccg tcgccgctgg cgctggacgc gctgcacgcc tcccgtgagc aggggcgctt
     6361 cgtcgtcgcc gtgaccagcg tcgagatcct cgagtccctg ctcgcgctcg tccccgagac
     6421 catcgctccg tggctgcgtg atgctccact gctgctgccg ggcgagcggg tcgctgccga
     6481 ggcactgcgc cgcggctggc gcggcccgct gatcgtggcc cggagcgccg aagatgagac
     6541 catggtcgag acgcttcggg actgggtcgt gcacggactc ggttccgggc gccggtgacg
     6601 gatcgcgtgc gtgatacgat ccgcctggca ctccgggcgc ccgcgcagag ccgatgtccg
     6661 ccgaaatcga atcgctgtcc agtgacatga gttcccgccg cacgggcggc ggcacgagcc
     6721 cgatgctggt ggcggtgtcc gtgctcgccc tgctgctcgc cggatgggca cactggcgct
     6781 tcaatcactt cgacgaccgc gtcgacaagg tccgcaggca ggtcgccgag ctgcgcgaat
     6841 cccgcaaccg cctggccgcg cagatctcgg cgctgacgac gcgcctcgag acctcgcaga
     6901 atgcgatgcg cgcggagctg ggcgcgctca aggagctgcc cgctcagctt gcagtgctcg
     6961 ggcgcggcgt cgaggaactc aaggcccgca ccgaggcgcc gcagcgtgcc tgggcgcgcg
     7021 cggaggcgct gtacctgctc gacctcgcgc agcgccagct cgacctcgac cacgacatag
     7081 gcaccgccat cgcggcgatg gaggccgcgg acgcgcgcct cgcggccttc aacgaccccg
     7141 ccatgggcga ggtgcgacgg ctgctggcgc aggacctcgg ggctctgcgc gcagtgaccg
     7201 tgccggacct gcctgccgtg ctcgagcgca tcgcagccgt cgaaggcgcc gccgcgacct
     7261 tgcccgtgct gggcgtcccc gtggcccgcg aggcgaccgg cgcaggggag ccggccggga
     7321 ccagcgcgtt ccggcgcgcc ctgcggcgca tcgccgacgc gttgagcgga ctcgtgtcat
     7381 tccggcgcgt ggaccccgcc gccatgcagc tcgtcacgca ggaggagcag tccctgcgcc
     7441 gccagcacct cgagctgctg ctgctcgccg cgcgcgtcgc agccatgcag ccggacagct
     7501 cggcgtacac gagttcgctc gatgccgcga gcaagtggct cgaccagtat ttcgacggat
     7561 cgtctccggc ggtcgtggcc gcacgcaagg aagtcgctgc gctgcgcaac gtcagcgtcg
     7621 agccgccggt gccgaagatc ggggccgccg cgcgccagct gcagctcgtg atgcacggcg
     7681 gcacgagcgc gccatgaggg gcgcggtgat cgtggcgctg gcgctcgtgg gcggcgggct
     7741 ggttgcccag ttgctgctga ccgatcccgg ctatgtcgcg gtacgtgcgg gccgctcgct
     7801 ggtcgagacc acgctgcccg tgttgctggc attcctggcg gcggcctacc tcgtggtgag
     7861 gggcatcgcc gggatcatct cggcccgtcg ccgcctcgct gaactccacg ccgagcgccg
     7921 ccgacgtcgc gcacgcgacg acacgcgccg cggcctgctc gacctcgcgg ccggcaactg
     7981 gcgccgtgcc gaggaactgc tcacgcgttc ggcggccgac gcggactctc ccgcgtcgaa
     8041 ctacctcatc gcggcccgcg cggccgacct gctcgacgcg atcgaccgtc gcgaccagtg
     8101 gctcacccag gcgcaggaga gcgcgccgga ggagcgtgcc gcggcgctcg tgaccctggc
     8161 cgagatgcac ctgcgccgtg ggcaggacgc cgcggccgtg cgcaccctcg agcagctcga
     8221 ggcgtccggc gacctcaatt cgcgcgggct cgagctgctt gcgcgcctgt acccgaagct
     8281 cggccagggc gagaagctcc tcgcgctcgg tccgcgcctc atggccgcca aggaactgcc
     8341 gccggcgcag gtgagcgagc tgctcgcgca ggtgcagctc gaggaaatac gtgcggccgg
     8401 cgcacacaac gaccaggcac ggctcgagaa ggcctggtcg gacctgtcgc ggggcgtgcg
     8461 ccggctgccg cagtcgatcg tcacctacgc gcgggccgcg atgggctgca acgatcaccc
     8521 aacggcggaa aaggtactga gggaattgat cgacgacacg tcggacccgg cagcagtgcg
     8581 gctctacggc gacctcgtgc tggccgatcc gctcgcgccg ctcgatcgcg ccgagggctg
     8641 gctgcgcaag cggcccgagg accccgagct gctggcgacg tgcgcgcggc tctgcctgcg
     8701 ggcggaactg gtcggcaagg cgcgcagcta cctcgaggca agcctggcgc ggcggccgag
     8761 ctcggaaaac agcctgatcc tcgccgacct gctcgaccag atcggcgagc gcgaccgcgc
     8821 ggccgactgc ctgcgcaagg ccgtcaccct gtccgtcggc cggcgtccca acgtgccgcg
     8881 cgtgcgcctg cggcgacgct gagcgatcgt caggaagtcg cggtcgacgc ggacatcttc
     8941 gccatcgtgt cgggcgcgta atcgaacgaa tcgaagtgga gttgctcgcg cggcaggccg
     9001 gccgccggga actgctcgcg tatggtctcg atcatgctgg gcgggccgct cgcgtagacc
     9061 tcgaatgccg acaggtccgg gtggtcggcg agcaccgctt cgtgcaccgg gcccgaacgc
     9121 acgctcgccg cgatcggctg cggctccgac agcaccgggc aatagcggaa tcccggccgg
     9181 ctccgctcga ggtcccgcag ccacgcgtgc tcgtacaggc cgtccaggtc gcgcgagccc
     9241 cagtagagcg tcaccggccg ccggtcgccg atggcgatca tctggcgcag catcgcgcgc
     9301 agtggcgcgt accccgtgcc gccgccgacc atcagcgtcg gacgtggcga ttccgcccgg
     9361 agccagaact gtcccagggg accctcgatg cgcagcagcg cgcccggacg cagcacgtcg
     9421 aagagctgcg ccgtgaagcc gctggtcgag gcgcggcgca cgtgcagctc gacgagcggg
     9481 ccgtcggccg gcgcgcttgc gatcgaaaaa ctgcgccgcc ggccttccga cagcatgatg
     9541 tcgaggtact ggccggcgcg atgatgcagc gcctcgaccg cgggcagccg caggaacacg
     9601 gccatgacgt cgggcgccag cagcaccttg cgctcgatgc gacacggcag gctccggacg
     9661 tccacctcgg gcgccggacg catctcgcgg gcctcgaccc gcaggtccgt gagcgcacgt
     9721 gcctggcaca gcagcgcgta gccgcgacgc gcttcgtctt cggtgatgcc caccgggcgc
     9781 tcgccgtgat acgcgatcgt gccggacacg atccgtgcca ggcacgacga gcagtggccg
     9841 cccttgcagc tgtggggcag gttgagtccc gccctcagcg ccgcggcgag caccggttcg
     9901 tcgcgctcgg cggtgaagtc ctgacccgac gggaccagcg tgacctgcgg catgcgcgca
     9961 ggatgccacg aaggacggcg cgagcgtcag cactcgcgcc gatgccgccg tcgctgctcg
    10021 cgtatccttg cgggacatgg atcgccggtg cgtcatcgcg ggttgcggct acgtcgggag
    10081 gcggctggcc gtgtctgtgc agcggtcgtg gcaggtcgtc gcggtgacgc gagcgccggc
    10141 gacggtcgcg gacctgcgcg cggcgggtat cgagacgatc ggcgtggatt tcgattccga
    10201 ccaggctgcc cccgaattga ggagcctggc cgacggagcc gctgtggcgt acctggcgcc
    10261 gcccccgaac cacggcacca cggacccgcg cctcgagcgg ttcctgggcc tgctcgccga
    10321 cgcgcgcccg gcggtgctgc tgtacatgag cacgaccggg gtctacggcg acgcggcagg
    10381 agcggcggtg gacgagtcga gtccggcggc tccgaccaat gaccgctcac gtcgccgcct
    10441 ggccgccgaa cgtgtcgcgc agtcctggtc cggtgcccgc ggcacccgct gcgtgatcct
    10501 gcgcgtgccg ggaatctatg gcccgcatcg cttgccgctc gagcggctgc agcgcggtga
    10561 gcccgtgctg cgcgccgagg actccggacc cggcaatcgc atccacgtcg acgacctggt
    10621 cgcgtgctgc atcgccgcca tcgagcgacc cgtcgacggg gtgttcaacg tcggcgatgg
    10681 tgatcacgcc agcacgaccg cgttcctgca gaaggtcgcc acgctggccg ggttgcagtc
    10741 gccgcgactc gtctcgctgg cggaagcacg aggccagatc agcccgggga tgctcgagta
    10801 cctggtcgag tcacgccgcg tcctcacgac gcgcatgcgc gaggaactgg gcgtgatccc
    10861 ccgttacggg atcctggagt cgggaatcgc ggcgagcctg gccgagatgc gcatccgctg
    10921 atcgcgttcg gatatcctta cgccatgcgg ctcgcattca cgaagatgca tggactcggc
    10981 aacgatttca tcgtcttcga cgcccgggcc ccgggcgagg tgccctcgcc cgccgagctg
    11041 cgccgcctcg cggcgcgccg caccggcatc ggcttcgacc aggcgctggt cctcgaaccg
    11101 ccgcgccgcg acggcacgga cgtgtactac cgcatcttca atgccgacgg ctcggaggtc
    11161 gagcagtgcg gcaacggcgc ccgctgcatc gcgcgcctcg tcgccacgcg cgcgtcgcgg
    11221 caggatcgcc cgctgcggct cgacagtccc ggcggcgtcg tcgaggcgcg cctgcgtcgc
    11281 gacgggctgg tgtcggtcgc gatgggggtg cccgatttct cacccgcgtc gctgcctttc
    11341 gtcgccgatc gcgaggagcc gagctaccac atcgagctag gctctggccc gatcgagttc
    11401 ggcgcggtgt ccatcggcaa tcctcatgcc gtgatccgcg tgcgttccgt cacggatgcg
    11461 ccggtggaca ccgtcggacc cgcgatggag aatcacccgc gctttccgcg gcgggtgaac
    11521 gtcggattcc tcgagatcgt cgcacccgac cacgtgcgcc tgcgcgtctt cgagcgcggc
    11581 gcgggcgaga cacgggcatg cggcaccggg gcgtgcgccg cggtggccgt cgcgcggaag
    11641 catgggccgc tcgccgagga ggtccgcgtg gacctgcccg gcggacggct ggtcgtccag
    11701 tgggccgggc cgggggaaca ggtctggctc acgggaccgg cggagacggc cttcgaaggc
    11761 caggtggatg tcgcagacgc gccctaaggg gagacctcaa gtgaacaagc agccgatacg
    11821 cccgaccgga ccggaagcga tcgccgagga gcaggtcggc gactacctcg tcgcgcaccc
    11881 ggagttcttc gagcggcatc ccggcgtgct cgcgcgcctg cggctcccgc accagcgcgg
    11941 cagcgccgcc gtctcgctcg tcgagcggca ggtgctcgtg ctgcgcgaga agcacggcgc
    12001 gctcgagcag aagctgcagg agctgatcga aaacgggcgc gcgaacgacg ccatcgccga
    12061 ccggatgcac cggctgaccc gcaggctgct gcgtgcgcgc gacctcgggt ccacgctcgc
    12121 cggtctcgag acgtcgctgc gcgaggactt cggggcatcg cgctggctcg tgctgctcac
    12181 cgattccgcg ctgccgggcc tcgcgggtca ccagcatccg cacgtgcgcg tcgtgccccg
    12241 cggcagtccc gaactcagga tcttcgagac cttcttcgag tcggcgcgcc cgcgcagcgg
    12301 gcagatccgc gacacgcagc gcgactacct gttcggcacg gagggcgggc ccgtgggctc
    12361 gaccgtgctg atcccgctcg gcgagcgcgc atcgctcggg ctgctggcga tcgcgagcca
    12421 cgacaccgag cgctacctgc cgaccatgag cacggacttc ctggtgcgca tcggcgacat
    12481 cgtcaccgag gcgatcacct cccgcgccgc atgagcgagg cgctcgcagg cggtgtgacc
    12541 cgcttcctgc agggcctgcg caacgagcgg cggctgtccc cgcacaccga gtcggcctac
    12601 gcgcgcgacc tcgagtcgct gatggagtat tgcgcttcac aagggatcgc gtcctgggac
    12661 cagctcgacc cgcagcacgt gcgcagtttc gccgctcagt gccaccgtcg cgggctgtcg
    12721 ccacggagcg tgcagcggcg gctgtccgcg gcacgcagcc tgttccgcta cctgatgcgg
    12781 gagggcgagt tgtcgcgcga ccctgcgacg gacgtgcagg cgcccaaggc ccgcaagcgc
    12841 ctgcccgtga cgctcgacgc cgacgcgatg gcgcggctgc tcgagtttcg cacggacgat
    12901 cgcctcggcg tgcgcgacaa ggcgatcatg gagcttttct attcctcggg cctgcgcctg
    12961 tcggagctgc tgggtctcga cctcaccgat ctcgacctcc gcgaccgcac cgtgcgcgtg
    13021 ctgggcaagg ggcgcaaggc acgcatcgtc ccggtcggca ggcacgcggc ggaggcgctg
    13081 tcgcgctggc tgcgcgaacg gacgacgctc gccgacgtgg gcgagactgc ggtgttcgtc
    13141 ggcacgaacg gccggagact cggcccgcgc atcgtgcagc gacgcatcgc gcgctgggcg
    13201 cgcctgcagg ggctgccgga gcacgtgcat ccccacatgt tccgccactc gttcgcgtcg
    13261 cacctgctcg agtcgtcggg cgacctgcgc gcggtgcagg aactgctcgg ccatgcgaat
    13321 atctcgacga cccaggtgta cacccacctc gacttccagc acctcgcgcg ggtctacgac
    13381 tcggcgcacc cgcgcgcgaa gcgcagaagg gcttagcaag acagaatccg gtgccaggca
    13441 ccgaccccgg gtattttcat gacaagcttc gatccacacg gcaccaccat cctctgcgtc
    13501 cgccgccacg ggctcgtcgc catgggcggc gacgggcagg tgacgctcgg caacaccgtg
    13561 atgaagggca acgcccgcaa ggtgcgccgc ctgcacgagg ggcaggtcgt cgccgggttc
    13621 gcgggcggta ccgccgacgc gttcacgctc ttcgagcggt tcgagggcaa gttgcagcag
    13681 tacggcaacc tcacgcgcgc ggcgatcgaa ctcgcgaagg actggcgcag cgaccgcagc
    13741 ctccggcgtc tcgaggcgct gctgtgcgtc gccgacgcgt cgcactcctt catcgtctcg
    13801 ggcaacggcg acgtgatcga gcccgagcac gacctcatgg cggtggggtc tggcggcgcg
    13861 ttcgcgcagg ccgcggcgac agccctgctc aggaataccg acctcgacgc gcgcgcgatc
    13921 gtcgagcagg cgctcggcat cgcggccgac atctgcatct acaccaaccg caacctcacg
    13981 atcgagacgc tgggaaagcc gagcgccggc tgaaggccgc ggggccggag gcgacggtcg
    14041 acacggagcg atcgccgcct ggcgacgacc tgcgtggctc aggtcccgcg catcgcccag
    14101 ccgagcatca gcaggccgct cgcggcgatc cagctgccgg cgaccctcac tgcgatccgc
    14161 gcccagtgcg cgcgcagccg cacgacgaat gcgctcacga gcgcaatcgt cacgaagacc
    14221 gccgacgtca gtccgagcat cgcgacgaag gactgcccgg accggcccag gcccgacccg
    14281 ttcgggtagc cgtgcgcgag gccgagcacg acggccagcg cggtcagcac acggggcgac
    14341 agtttcgcat cggcgaccac gaggccgccc aggaccagga agttcagcgc cgcccaggcg
    14401 gatccgggat tcacgaccgg cgcatccatg ccgagcaggc agcccgcgag ccatgcggcc
    14461 ggcaacgcga acaacgcgcg cctcgcggcg tccgcgccat tcaggccagc cagcaacgcg
    14521 accgccagga ccggcacgag gtcgtccggg ctcatcgcga agtgcgcgag cccgtcatac
    14581 accgggccca tgccggtgga attcaggtga gcctcggcgg gtgcgcagcc cagcgccgcg
    14641 gtcagcgccg cgatgcagcg agcgaagcgc gaaccccgcc acgcacgagt cacgcgatcg
    14701 ccctccacag gaaatacacg ccgcccgcca tgaccactgc gcccgcgccg cgcagcagcc
    14761 tccgtcccca gggccaacgg tgcgccgttc cgatcccgat gcccacggcg tgcaggcacc
    14821 cggtcgccac cacgaatccc aggctgtaga gcatggcgct ctgccccggc ggcaactccg
    14881 tcccgtgcgc gtatccgtgg aagatcgcga agatgccgac cagcagcgcc gcgacgacga
    14941 gcggcgggcg tacctcgaac gctacggccg cgccgagcac gatcgccgac gccgcaatcc
    15001 cgtactcgat cccgggcagc ggcaccccga gaaagccgag catcccgccc atcgccatga
    15061 cgaggggaaa cgccacgggc agcacccaga tcgcgggcgc cccgagctgg gcaccccaca
    15121 gcccgaccgc gaccatcgcc agcacgtgat cggtgccgga cagcgggtgc atgaacccgg
    15181 tcagcaaacc gtaggcctgg cccttggtga cgtgtgccag cgccgccggc gtccagcagg
    15241 cgatggccat ggcggctgcc cattgccccg cgcgcgacga ccttcggccg ctcatcgcat
    15301 cgccccgaac atcaccgcca cgcgctggat ggtccagaac gcgcccagtg cgcccaccgt
    15361 gtacccgggc aacgcctgca cccaccgcgg ccagcgcacc cgcaggatcc ggaacgaccg
    15421 ctccagcgcg agcaccagcc cgacgaacgt gagctggccg atttcgacgc cgacgttgaa
    15481 cgagaacaga gcgatcggca cgtcctgctg cggcaggccg gcgctggtga gcgcgctcgc
    15541 gaagccgaac ccgtgcagca agccgaaggc gaacgccacc acccaggggt ggcggatggt
    15601 gaagctggtc tcgccgcgcc agacgcgcac gatctcggga ccgaggaaca ggatgctgag
    15661 cgcgatcgcg gcattcagcg gcagcgcggg cgcctgcgcg tagccgagcg tcgcgatggc
    15721 gagcgtgatg ctgtgggcga cggtgaacgc cgtgaccgtc ttcacgagca tccagcggtc
    15781 ccgcacgatc agcagcaggc cgagcacgaa cagcaggtga tccgcgccga acaggatgtg
    15841 gcgcatgcct tccacgacgt aggctcccat cacctgccag cgggtttgcg acgcggcgat
    15901 ctccacccac gggtgcgagg gccgcgcgat cgcggtcgtg gtggtgccgt cgagccgctc
    15961 gacccgcacg aggacgtcgg tgatggtcag ctgcaatccg ggaaagtcga tgcgccggcc
    16021 cgccagcccg cccttgccgg cgtcgatcca gcggcgctcg acgacggagt ccgcgagctg
    16081 gtcgatctcc ggggaacgga cgttgcgcac gtcctgcggc agctgcagca cgacgggcag
    16141 ccgcatcccc gcgagcatcg gcgtgcgcca cagcagcgag tactggccag gcgcggtctc
    16201 cctgacctcg aggtaggcag gccgcgattc gtgggcctgg gccggcggca cacagccaag
    16261 cgccgcgacc agcatggcca cgatgcggac ggcgacatgg cgcaggctgg attgcattcg
    16321 atgcctcagt ccgccgtcgg ttgcggggcc gcggccgggc gctgcggcac ggggatgtcc
    16381 gcggcgacgg cggccggcac cggcagcagc accgtgtact tctcgcgcat gtccttgtag
    16441 gccttggccc aggcctcggc cttgcgctcc gcgagccacg cggtcttcac gtccggctcg
    16501 acttcctcga agtccgggac gcggcccggg atgaccgtat cgacgtacac gagatgccag
    16561 ccgaatccgg actcgaccgg tccctgccac gaacccgccg gcagcttctc gaccgcctgc
    16621 gcgaactgcg gcccgaactc cttgccgaga tagtcgggcg cgcgatcgcg gtagtagtcc
    16681 tggaacatga acggatcggc gagcgtggac gcgagcgggg cgtcctccgg ctttcctgcc
    16741 agctgttcca gcgcgcgcgc cgcgtcatct cgcgcccgcg tcccgcgccg gtccggcgag
    16801 aagtacaggt gacggaagct gatgcgcggc ggcatggcga accgctgcga gttcttggcg
    16861 aaccacgcct gcagttcgtc cgtcgtcggt tcgcgcgacg tggcgacgtc ctcggcgagg
    16921 aactgcatct tctgcgccat gcggcgcttg acgatctcgt cgtcctggtc gaggcccatg
    16981 gcgatcgcct cgcggtacag gatctcctgc tgcaccttct cctcgaccag cagcgagagc
    17041 tcctgcgggg tcggttcgcg ctgccactgc gagcggaaca ccgcgatcat ctgccccagg
    17101 tcgtcgaggg tgaaccggat ctgcttcgac gacggccccg cgtcggtcgt cggcccgtag
    17161 taccggtaca gcccgaacag cgcggccccg agcagcagga aatggacgag cggctcgcgc
    17221 aggaagcgtc ggatgcggtt catggacgtg tcatcggcgg cgtggccgcc gtgggcggcc
    17281 gtcgtggtcg tgttcttcgg ggtcgtcggt gcggtcccgc cggcaggcag cgcgaccggc
    17341 gaccgtcgaa gtcgggtcgt gcgccgagcc cgcgcggcgg aatcgcggct aatctagcac
    17401 agccgccgga cgcgaccgaa cgcgcggcgt ccagtatcct cggcacatgt cctcgatgac
    17461 tccccgcgaa atcgtccagg agctcgacaa gcacatcgtc gggcagggcg ccgccaagcg
    17521 cgcggtcgcc atcgcgctca ggaaccgctg gcggcgaatg caggtgcccg agcgcctgcg
    17581 cgcggagatc accccgaaga acatcctgat gatcgggccc accggcgtcg gcaagaccga
    17641 gatcgcgcgg cggctcgcgc ggctcgcgaa cgctcccttc gtgaaggtcg aggcgaccaa
    17701 gttcaccgag gtcggctacg tcggccgcga ggtcgactcg atcgtcaagg acctcgtcga
    17761 cgtcgcggtg aagatgaccc gtgagtccga gacggacaag gtgcggcatc gggccatgga
    17821 cgcggccgag gagcgcgtgc tcgacgtgtt gctgccgaag ccgcgcaatg tcggattcgg
    17881 cagcgaggcc tccgctgacc gcgacggcga cactcgccag cgcttccgca agatgctgcg
    17941 cgagggacaa ctcgacgacc gcgagatcga gatcgagttg cgcgccgtgc cgatgggcgt
    18001 cgagatcatg gcgccgcccg ggatggagga actttcgcag cagcttcagg gaatgttctc
    18061 gagcctcggc ggcgaccgca cgcggccgcg caaggtgaag gtccgcgagg cgttcaaact
    18121 gctgaccgag gaggaagccg cgaagctcgt gaacgaggag gagctcaagg tccaggcgct
    18181 gcgcaacgcc gagcagaacg gcatcgtgtt catcgacgag atcgacaaga tcgcgcgccg
    18241 gcaggagacc gtgggtgccg acgtgtcacg cgaaggcgtg cagcgcgacc tgctgccgct
    18301 ggtcgagggc tgcacggtca acaccaagta cggcatggtg cgcaccgacc acgtgctgtt
    18361 catcgcatcg ggcgcgttcc acatgtcgaa gccgtccgac ctcatccccg agctgcaggg
    18421 ccgactgccg atccgggtcg agctcgaggc gctcggcgtg gacgacttcg tccgcatcct
    18481 caccgagccc gatgcctcgc tgtgccgaca gtactcggca ttgctcgaga ccgagggtgt
    18541 gcgggtcgag ttcgccgccg acggcgtgcg ccgcctcgcg gaagtggcgt tcgacgtgaa
    18601 ccagcgcacc gagaacatcg gcgcgcgccg actgcacacg gtgatggagc gcctgctcga
    18661 aggcctgtcg ttcgaggcgt cggaccgttc gggagcgtcg gtcgtgatcg acgcgtccta
    18721 cgtggaccgc aacctcggtg aactcgcgcg cgaccaggac ctcgcgcgct acatactgta
    18781 ggcccgcatg gcggatccac gcccgaccga catcaagctg cgcacgcgct cgcgtgtgct
    18841 ggaaatcacc ttcgatgacg gcgagcgcca cgaactgccg ttcgagtacc tgcgcgtgta
    18901 ctcgccgtcg gccgaagtgc gcggccacgg gcccggccag gagacgctgc aggtgggcaa
    18961 gcacgaggtt ggagtcaagg ccgtcgagac ggtcggcaac tacgcgttgc gcatcgtctt
    19021 cgacgacggc cacgacacgg ggctgtacac ctggggttac ctgcaggagc tcggccgcac
    19081 gcgtgccgag aagtggcagc gctacctcga ccggctggcg aagctcgggc tgccctaccc
    19141 gacgccggtg ccgacaagat tgcctcgcac gcagtgaatt catcagggag cgcggacatg
    19201 gctcgtccag tcagcccgat gattcccggc atcaaggccc tggtcctgtg gctcggcttg
    19261 accgccgctg cggtcgtggt cgccgacgac ttgcggcagg ctcacctgtt ccttcgcagc
    19321 aatcacatct acatccggat cccggacggc cccgaatatc gggtggaggt cgactctccg
    19381 gtttacgagg aatacgcccg ggagcacggt gccgattgcg agactccgcc cgaggtacgc
    19441 gagaaggtcg aaattcgcca gttctacaag ccttcgaagg gcggcgtgcc cggacgcgtg
    19501 cgcgtcgaac tgctggtggg cccggccgga cgggtcgtcg cgatccaggc cctcgagtcg
    19561 ggtgccgggg gactttcgct gtactccgtc ctctacgcga cccgacgatg gatatttgag
    19621 ccaggccgcg accgaagtgg ccaggccgca ttctgccggt accccgtgtc ggtcgagttt
    19681 gtcttgcccg acactcaggg ctgacgggac caggcggctc gagacgcggg gtcgcccgat
    19741 ccgcgtagaa tgcccgttcc catgagcgaa ccgtccgaca agcaagccgc ctccggctcc
    19801 gtggacttcg gctacaagca ggtgccgaag gacgagaagg cgaagcacgt ccgcgccgtc
    19861 ttcgactcgg tcgcagacaa gtacgacctg atgaacgacc tcatgtccgc cggcgtgcac
    19921 cgcctgtgga agcggttcgc gctgtcgcag acgggcttgc gcccgggcca ggcggcgctc
    19981 gatgtcgccg gaggcacggg cgatctcgcc gcgggcatgg cgcggcaagt cggcgacagc
    20041 ggcctcgtcg tgctctcgga catcaacgcg gcgatgctgg aggtcggccg cggccgcctg
    20101 atggatcgcg gcctgatgcg caacgtgcgc ttctcgctcg ccaacgccga gtgcctgccc
    20161 ttcgaggacg agagcttcga ctgcgtgacg atcggcttcg ggctgcgcaa cgtcaccgac
    20221 aagcccgctg cgctggcctc gatgcgccgc gtgctgaagc ccggcggccg gctgctggtg
    20281 ctcgagttct cgaagcccgt cgtgccggga ctcaagccga tctacgacac ctactcgttc
    20341 cgcgtgctgc cctggctcgg gcggcgcgtc gccggggatg ccgacagcta ccagtacctc
    20401 gccgagtcga tccgccgctt ccccgaccag gagacgctgc gcacgatgat gggcgacgcc
    20461 ggcctcgagg actgcagcta ccacaacctg agcggcggca tcgtcgcgct gcacaaggga
    20521 ttccgttact gacatgctgc tcgagcccct cggcgacctc ctgaaccgca acatcgcgga
    20581 ctcgcgccgg gcgcaggcgc tctgccggca gctcgacggc cgcacggtgt cgctcgtcgt
    20641 cgagggcacg ccgctcgcat ttttcttcag gtcggagggc gggcgcgttg ccctgaccgc
    20701 gagccacgac ggggccacgg atgcgaccct gtccggcaca ccgatcgggc tcctcgcgct
    20761 ggccggcccg cgcgccgagg gcgcgctgcg cggcggcggc gtgcgcatcg agggggacgc
    20821 cgaggttgcg cagaagttcc gcgacctcct cgcggaatcg cagccggact tcgaggagga
    20881 actggcgcgc gtgatcggcg acgtggcggc ccgcaacatc gcgaacctcg cccgcggatt
    20941 cctcgacttc ggccgcaagg ccggtcgatc actcgccggc aacgtgtccg agtacctgca
    21001 ggaggaaggg cgcgacgtgc cgacccgcac cgagatggag gagttcctct ccgacgtgga
    21061 tcacctgcgg gacgacgtcg agcggctcga tgcgcgactc gcgcgcctcg aatcgggtgc
    21121 cggcaaaccc gcgtcgccgc aaggcgcgac gcggagcaag gcgcagtgac gggcagtcac
    21181 gcagcgcccc gcacctgaca ccgcaaccca gccacgggcg accagcattc cccccatgcg
    21241 cttccgcgtc atcggccgac tcctgcagat ccagcgcgtg ctcgtgcgcc acgggctcga
    21301 cgagatcatc ctcgcgaccc acctgttccg gccgctgcgc ttcttcttct acctgtcgcc
    21361 ggcgacgtgg ttcgagcgcc ggcgtggcgg cacgcgcggc gagcgcatcc ggctcgcgct
    21421 cgaggaactc gggccgatct tcgtgaagtt cggccaggcg ctctcgacgc gccgcgacct
    21481 gctgccggcg gacgtcgccg acgagctcga gaaactgcag gaccgcgtgc cgccgttccc
    21541 gggcgccgag gcgcgggcca tcgtcgagcg cgcctacggt cgctcggtga ccgaggtctt
    21601 cgagcagttc gacgagagcc cgcttgcggc ggcatcggtg gcgcaggtcc acaccgcgcg
    21661 cctgcgcacc ggcgaggagg tcgtcgtcaa ggtcatccgc ccgggcgtgc gggagaagat
    21721 cgagcgcgac ctcgaggtca tgtacgtgct cgccgggctc gcccgcgact acgcgagcga
    21781 aggccaccgg ctgcggccgg tcgaggtcgt gcgcgagtac gagaagacga tcctcgacga
    21841 gctggacctc atgcgcgagg cggcgaatgc cgcgcagctg aagcgcaact tcgcgggcaa
    21901 cgagctgctg cacgtgccgg cggtgtactg ggactactgc cgccccgacg cgatggtgat
    21961 ggagcgcatc cacggcgtca tcatcagcga catgcccgag ctgcgccggc gcggcaccaa
    22021 catccagcgg ctcgcggaaa gcggcgtgac gatcttcttc acgcaggtgc tgcaccacaa
    22081 tttcttccac gccgacatgc accccggcaa catcttcgtc ctgctcgagg accccgagca
    22141 gccgaagtac tgcgccgtcg acttcggcat catgggcacg ctcaccgcgc gtgaccagca
    22201 ctacctcgcc gagaacttcc tcgcattctt cgagcgcgac tggcgccgcg tcgcccagct
    22261 gcacgtcgac tcgggctggg tgccggccga gacgcgcgtc gacgagctcg agaccgcggt
    22321 gcgcaccgtg tgcgagccga tcttccagaa gccgctgtcc gagatctcgt tcggccaggt
    22381 gctgctcagg ctgttcgagg tggcgcgcag gttccagatg caggtgcagc cgcagctcat
    22441 cctgctgcag aagacgctgc tgcagatcga ggggctcggc cgccagctct acccggagct
    22501 cgacctctgg aagacggcac agcccatcct cagggactgg gccgccgacc ggctgagtgg
    22561 ccgcgcgctc gcacaggacc tgcgccggca gctgccggac ctgagcgagg cgttgcgcat
    22621 gctgccgcaa gtgctgcaac aggtgctgca acacgcctcc gatggcacct tccgactgaa
    22681 ggttgagcag gctggcctcg agcagattcg cgaggagatc cgccgcggcg cacgacaacg
    22741 tgatgcgacc gtcgtcgcag cggcggcaat gctcgccggg ctcgtgtggc tcgccatcgg
    22801 gatgtcggcc tggcccggga ttgcgctgtg tgccggggga ctcgtggggg cgctggtcgc
    22861 gcgccgctgg tagcggccga aggtccggcg aacaatggcg cctcagggtc cgcccaccgg
    22921 cagcttcacc tcgatccacg tcgcgagcgc ccgcgcgcag agttccccgt cctcgtcgaa
    22981 gatcgccgtg ccggcatggt gcttgcggcc ttcgctgccg atcttccagc cgatgacggt
    23041 gcaggattcg ccggtgtgca cgcggcgatc gatgtgcgcc tgcatctcgc cgagcaccat
    23101 cgggcggcgg cccgccgaca cggcgaagta ccccgggcaa tcgagcgcgg cccagtgaaa
    23161 cacggccgcg accctgccgt caccgcgatc gaggtcgtcg gccggcagcc agggcgctgc
    23221 gacgattccc gtatcgagca tgcccgggaa gatccgcagc ccgtcgccgc gccgccggtg
    23281 cgggccgcac acgaagcagt ccgggaatgc gtgctggtgg aaccccgggt agtgctgcga
    23341 ggcccagacc gcctggacgt acgcgggtgg cgacggcacg tcgagcacca gcctgcctgg
    23401 cgtgcccact gcgacgggac ccgcctgcga cgcgagcgtc cagctgccgt cgtccttgcg
    23461 ctccagcgcc atcgcctcct cgaggggcgg cggcgcgagc agcctcacct tgagatcggc
    23521 attcgcggcg acggccaatg cgccgcaagc atatccgccg ttcgccgaac cgggtggccc
    23581 gttgaagcga cgtccgatcg agagcgtgct catggctcgc ccgtcccgcc atcggctctc
    23641 gccgcacagt tccagcattc ggtaaattgt ccttcgcagc gctcgccgca gcgagcacac
    23701 gtccaaccct ccgaatcctg ggccggccgc agcgcctcgt cgatcagtcc cctggcacgc
    23761 agcgcgtgac cgggctcgcg gacccacagt tcgggccagc actcgacgaa agggatctcg
    23821 ccgatcacgc ccgagagccg gtcattgcga acgacgcacg gaatgccggc tgactcgagg
    23881 acgttgcgca ggtgcgcgat ctcgatcgtg gactcggcgg tgtggacctt gcgcatggca
    23941 gagccccggg cctcaggcct tccgcggccg gtcgaccgcg aggatgaagc gcgtgtagat
    24001 ctccggcagg cgctcgagca cctgcgcctt gcccagcatg tcactcccgg cgacggcggc
    24061 ccgcagcacg ggcagcgtga gcgcctccag cgcttccgtg gcgatgggcc agtagctcag
    24121 gtgccagggc tcgacaccgg cgccgctgcc gaccgaggcg tacggccggt agaacccgaa
    24181 gcgcgtcatg ttcgagtcca gccacgccga gagtcgtccg aacacgccgt cgccggcgta
    24241 ttccgcgggc acgagctgga cctggtagcc ggccggcatg gcggctgcgt cgatcacgtc
    24301 gacgtcggtg ccccagtggt ggcgactgcc gcccgggatg gccgaccagc acaggattgc
    24361 gtccaccttg cgcgcgtcgt cgagcgtcga cgggtcgatc ggcttcccgg cacgatcctg
    24421 cagcggccgc tcgccgcgcc acttgcgatt ccacaggtga acctgtcgct cgaagtcgcg
    24481 gaagctggag gccgccgcga ggtccaggcc ctccgcggcc gcggcgtcgc gcatcgccag
    24541 gaacgacgtg acggcttcga cgtgcagcac gcagcgcgga tgctcgatgt ccacgacgtg
    24601 cgtgcgtgcg cggccggtca gttcgagttc gttcatgcgc gggggtcccg gcgggcaagc
    24661 ataccggagt cgcgaaccgc ccccgccggg cgcgggatgc cgcactgtcg gaaaattttc
    24721 catatgatca attggttgcc cgtcgctatc tcggctcgcc ctgccgaccc ggcgggtcta
    24781 tgatgaactc ggagccgggt gcgggctcca acaagaataa ggcggttgca ggtcgggctc
    24841 gagcgagccg gaaatccggt ccaggcatgc gcgggaatcg tctcggacat cgatatgtgc
    24901 tgacagtcgt cgcggtgggc gtgctgctca ccgccgcggt gtccgccatg gtcctggaaa
    24961 ccgcctctgc cgacgcccag cgcgtgcgca acgtactcgc gcaggcgcaa gaggtcgccg
    25021 cggcagatcc gaccgccgcg ctcgcgatcg tcaccgacga tgcgatgcac tccatccaga
    25081 ggatcgtctg gcgccgcctc ccgccggtga tcgcatcggg cctgctgctg accctgctcg
    25141 ccggcttcgt cacgtggcgc atggtgctgc gcatcgatcg cggcgtggag gccctcatgc
    25201 acagcgccga gcagatcggc agcgggcacc aggcggaacc cgtcactgcg acgggtgtgc
    25261 gcgagatctc cgcgatcgag gaatcgctcg agcgcatgcg ccaggcgctc gcccagacga
    25321 ccatttcgcg cgactacctc ggcatcgtcc tgaacagcat gagcgatgcg gtgctcgtca
    25381 cgtcgacgga cggccgcgtg cgaaccgcga atcgcgcggc gacgcagctg ctgggcctcg
    25441 gactggaccg gctggtcggc gtcgatctcg tatcgctcgt cgcggaggat caccgcgcga
    25501 ccttcgaccc tgccaaggtg ctcgcggcgc cgggcgaaac ggtgctcagc acggcgcgcg
    25561 gccagacgat ccccgtgtcg gtctcggcgt ccgccatttc gagcgacgac ccccgcttca
    25621 agggcagcat cttcgtcctg cgcgacatca ccgagcgcaa gcgcgcggag cggcgcatcc
    25681 gctacctggc gcgcttcgac acgttgacca agatgccgaa ccgcatgcag ttccagcacc
    25741 tgctgcacca ggcgatcacg cgcgcgacgc gccagggccg gtcgctcgcg ctgctgtacg
    25801 tcgacgtcga caacttcaag gacgtgaacg acacgttcgg ccacgacgcg ggcgatcgcg
    25861 tgctcgagac cctggccgag cgcctgagcc ggatcctgcc caaggagtcg gtggtgggtc
    25921 ggctggccgg cgacgagttc gcggtgttcg ccgagacgcc ggacggcgag caggaccggc
    25981 acgagcaggc ggcttccctt gcccgcatgc tgctcgccga gatcgcgcgg gcgcagcacg
    26041 tcggcgagca cgagatcgag gtcaccgcca gcatcggcat cgccgcctgc cccgatgatg
    26101 gcgacaacgt catcgacctg atccgcaacg ccgacgcggc gatgtaccac gccaagcgcg
    26161 ccggccggaa ctgccacgtg ttctacgcgc ccgagatgaa tgctgcggcg gtcgagcggc
    26221 tgctgctcaa gggcaagctg cgacgcgcgc tcgggcgcaa cgagttcgtg ctccgctacc
    26281 agccgaaggt ggacctcgcg agcggccacg tggtgggcgc cgaggccctg ctgcggtggc
    26341 ggctgccggg ccacggcgag atcgcgccgg cgcagttcat cccgctcgcg gaggagtcgc
    26401 tgctgatccg cgacatcggt gcctgggtgc tgaaccaggt gtgcagcgac tacgcggtct
    26461 ggcagaagcg cgtctcggac ccggggatcg tcgcgctcaa cctctcgctg aaggaattga
    26521 gccacgcgag cttcatcccc cgctgccgct cggtgttcca gcagcacggc gtgtcgcccg
    26581 gacgcgtcga gctcgagatc accgagacca ccctgatgat ggacgccgag cgcacgctgc
    26641 cgctgctcga cgagttgcgc gcgatgggcg tgcacctcgc catcgacgat ttcggcaccg
    26701 gctattccag cctgagcgcg ctgcagcagt tcccgatcgg cacgctcaag atcgaccagt
    26761 ccttcgtgcg caacgccgcc accgacccgg acgacgcgac catcgtgcgc accatcatcg
    26821 agatgggccg cagcctcggc ctgcaggtgc tcgccgaagg catcgagacc gaggagcagc
    26881 gcgcattcct gttgcacagc ggttgcgagt acggccaggg ccggctgttc ggcgagccga
    26941 tgagcgccga cgaattcctc gagaccatgg tgcggcaggc gaccggcgtg cacccggtgc
    27001 tgcggctccc ggcctgacgg ggaggaccgg tcaggttgcc gcggtccgcg tgcatctgac
    27061 gatgctcgcg gcactcgtgc gtatcagggc gtgccctggg ctgcggcggc aactcgcgtc
    27121 gcggcgtcgc gcacggcctg tcccgcggcc tgctcgaacg cggccaccac cgccgtacgg
    27181 tcgttgctgg cggctgggac cgacgccgcg gaccggaagc tcgccatgcg caagcccttg
    27241 cgtgcatcga cgaggttcgc ctcgaactcg acgacgacgg tgggcggagc gtccaccggt
    27301 gcgtacgccg cctcgaagcg ccgcagctcc acgtcgagca gcaggtccgg cggaacacga
    27361 ctcggcgccg cgactgcggt cgcgaagtgc ccgtccccgg ccagcgtgac gaccagcaac
    27421 tgctgcagca tctgcggcgc gggatcggcc cagcgcgcgc cggccaggta gtcgaagccg
    27481 ttgcccggcg tcgtcaccgc gatccggtcc gtgtcgagcg acgacggcgc gcgcggccgg
    27541 gcgactgcga tcgcgagctg cagcaccggc ccgcccggca cggcggccgg agcctcgagc
    27601 cggtattgct cggggggcgc ctcgttcgaa cgcagcgcgg tgccgacgca ggccgtgcac
    27661 aggctcgcca gcagcagcac cggccccaag gacctcatcg cgggatctcc atgccggatt
    27721 ccttgcgctg gatgatcagg ctcgaaggat tgtcgcgcag gctgcgggcg agctcgcgca
    27781 tttcctcgct ggtctcgcgc aggtcgccga tcagctgctg caggtccgcg accccgctgc
    27841 cggcgaattg cgtgagcgtg gcttcgttgc cgttgacgat ccggtcgagg ctgtcggccg
    27901 tccggccgag cttgtccacg gcgttgttca tgccggtgag cgtggcttcc agtttcgggc
    27961 cggcggtgcc ggcggcctcg cgcaggctgg ccgtgagcgc cgtcacctcg gttgcagcat
    28021 tgcgcagctc gcccgcaagc gccgccgcct cgtgcgtgac ggcgggcagg tcgcgcgatg
    28081 caaccttcac gttctgcagc gattgcgcga tggcatcgag attccggtcg gcaagcagca
    28141 actcgaggcg ctcggtgacg ccggccacgt gcccgagcag gtccggcagc ttggcgagga
    28201 acgcctcgat gtcgcccggc cgcgagggaa tcacggggaa ctcggtgcct ttcgccaatg
    28261 gtcgagacgg atccgccgcc gggttcttct gcaggtcgat gtacagcagc cccgtgagtc
    28321 ccagcaggcc gaggcgcgcc tgggttgcgc cggacacggg cgcctgggcg tccacctcga
    28381 cgaccgcctt cacccggccg ggattctcac gatccaggga caggcgcgtg acccggccca
    28441 cgtcgacgcc gaggtagcgc acgggactgc cgcgcgacag gccgctcacg gaaccgtcga
    28501 aatagatctc gtagcggacg tactgtcgct ggtcgccgtg ccccgagtac cacagcacga
    28561 acacgacggt cgcggccacc gccagcagcg cgaacgcggc gacggccgcg tatttcgcgt
    28621 cacgttccac cgtccgcccc ctgctgcgga accgccgcac ggccgcgggc gccgtggaag
    28681 tactggcgga tccacggatg gtcgttcgcg acgatgcctt cgagcgtgtc cacgatgacc
    28741 ttgccgtcca cgagcacggc gacacggtcg cacgtcctcg cgagggtgtc cagatcgtgc
    28801 gtcaccatga cgacggtgag ccccagcgcc ttgcgcaggt acagcaccag cgcgtcgaat
    28861 gcggccgccg cgatgggatc gagcccggcc gtcggctcgt cgaggaacaa cagcttcggg
    28921 tccagcgcga gcgcgcgtgc gactgcgacg cgcttcacca tgccgcccga gagctgggcc
    28981 gggtatttca ccgcggcgtc gagcggcagg cccaccattc gcaggcgcag ctcgaccagg
    29041 ctcgcgaccg tgtccggcgc cagcgtcatc gcctcgtgca tcggcagggc aacgttctcc
    29101 gcgacggtga gcgagctgaa cagtgcgccc tgctggaacg tgacgccgta gtgtcgcttg
    29161 atctcccgaa gctcggcgtc gcccatcgag gtgatttcgc gtccctcgat ctcgacggtg
    29221 ccggagtccg gatgccgcag gccgagcagc gtcttgagca ggaccgactt gccgctgcct
    29281 gagccgccca cgatgccgaa gacctcgccg tggtggacgt cgaagctcac gccatcgtgc
    29341 acgacctggg caccgaagcg gttgacgatg tcgcgagcgg atacgacggt ctgcacggcg
    29401 gatcctagaa gtcgatctcg aggaacagca ccgcgaacag cgcgtccacc aggatcacga
    29461 ggaaaatcga ctgcacgacc gccaccgtcg tgagccggcc gagttcgcgc gacgactcgc
    29521 gcacctgcag gccgcgcagc gtgcccgtca gcgcgatgac gagcccgaat accggtgcct
    29581 tgatcagtcc cgcccagaac gtcgtgtcgg cgatcgcgtc gtccaggcgc gccacgaact
    29641 gcggccacga caggtcgaac aggtaccgcg tgagcatcgc gccgccgacg aggcccatcg
    29701 cgtccgcgac gatcgtgagc agcggcatgg cgatcacgag gccgagcacg cgcggcagga
    29761 ccagtagctc gagcgggttg aggcccatgg actcgagcgc gtccacctcg tcgttcagct
    29821 tcatcacgcc gagttccgcc gcgtaggcgc tgccggaacg tcccgccacc atgatcgctg
    29881 tgagcagcac gccgagctcg cgcagcaccc cgacagcgac cacgtccacg gtgtagatct
    29941 cggcgccgaa cggacgcagc tgctgggcgc tgatgtaggc ggcgatgacg ctgatcagga
    30001 aggcgatcat cgcgacgact ggaatcgcgc ggacgcccgt ctcgtacgtg tgccgcacga
    30061 cggaagccac ccgcagcccc ttcaggcggg tccagcgcga cccggccgtg acgatcactt
    30121 cgccgccgaa ggtgagcgcc gcgtaccagc ctgtcgcgcg gacttcgagg tcacggccgc
    30181 gccccgcgag ccatggcagc agccccgtct cgggcgccgc cggcggcgca cctgcatcgg
    30241 aggccgcgag tccgtcgaga tacgcgaagt ggaccggcgg agtaccctcg agcgtcacct
    30301 ccgcaccccg cgcacgcagg cccgagatgg cttcgtgcag cagccaggcc gggccgatgt
    30361 cgaatgcccc ggcccgcgtg aagtcgatcg tgaggcgcgc cggcgcggcc tcgcggatcg
    30421 catcgagccg cgacgcgagc tccggcacgt ctgcgatccc gcagtcgccc gtgatgctca
    30481 gccggtcgcg cgcctcaccg agtaatgcct ggatggtcat gcatcacgtc tggtcgcagt
    30541 gcatgaatgc tagcatcggc cggtaggtgc ggtgaggacc atgaccggcg cagtgctctc
    30601 ccagatcctg atcctgctgg cgggctcggt catcgccctg agcctggtcc ggcgcttttc
    30661 gctgccgccg atcctggggt acctgctggt cggcctgttg ctcggcccga gcgcgctgca
    30721 cctcgcggtc gactccgcct ccgtcgcgtt gctcgcggaa ttcggcgtgg tgttcctcgt
    30781 gttcacgctg ggcctcgagt tctcgttcgc gcgcatggtc gcgatgaaat ccgaggtgct
    30841 cggcgtcggc ggcctgcaga tgctgatcac gaccgccgtg gtcgggagcg tcgcatgggc
    30901 gttcggcctc gcacccgccg tggcgctcgt ggtcggcggc gcgctcgcga tgtcgtcgac
    30961 ggcgatcatc ctgcggcagc tcggcgacca gatcgagctg aatcgcactc attcgcggct
    31021 cgccctcggc atcctgctgt tccaggacct ggccttcgcg ccgctgctcg cgctcgcgac
    31081 ttcgctcggc ggatcgggcg agatgcccgg cggcgcgtgg ctgctcggca tggccgcgcg
    31141 cgccgtcgcc gccctggtcg cagtgctggc gctgggacgc tgggtgctgc gtccgctgtt
    31201 ccacgagatc gcgcgccacc gcagcaccga gagcttcacg ctgaccgtgc tgttcgttgc
    31261 gctcggcggc gcctggtcga cgcacgcgct cgggctgtcc atggcgctcg gcgcgttcct
    31321 ggccggcatg ctgctctccg agacggagtt ccgtcaccag accgaggccg tcatcaagcc
    31381 cttccaggcc atcctgctcg gcctgttcct cgtctcggtc ggcatgctgc tcgacctcca
    31441 actgctgctc acccggctgc cgttgatcct gctggtgacc gtggcgctgc tcgtggtgaa
    31501 ggcggcgatc gttgcactcg tcgtgcgcgg gttcgtgccg aacacccgca aggcgctgcg
    31561 caccggcatc gtcgtcagca tgggcggcga gttcggcttc gcgctgctca ccctgctgct
    31621 gaagagccac atggccgacc ccgtcatcgt gcagccgctg ctgaccgcgg tcgcgttgag
    31681 cctgctcatc ggcccgctgc tggtgcgcta caacggccgc atcgcggacc gcatcctgcg
    31741 ccggccgacg gtcgaggctt ccgaggtcgc gcgggagacg gtcgctacgc gccagctcgc
    31801 gacccgcgag cacgtcatca tctgcggctt cggccgcgtc gggcagaacc tcgcccgggt
    31861 gctggaggcg cgcggcctcg agtacatcgc gctcgacctc gatccgttcc gggtgcgcag
    31921 cgcgcgccag gccggcgacc ccgtggtcta cggcgacgca tcggaagccg aggtgctgcg
    31981 agcgctcggc gtcgagcacg ccagcgtgct cgtcgtgtcg ttcgaggatc cgcagagggc
    32041 gctcggcatc gtgcgcaccg cacgtcaact gcgcgcggac ctgccggtgc tggtgcgcac
    32101 gggtgacgac gcgaagctcg aggagctgca gaccgcaggc gcgacggaag tcatccccgg
    32161 gacgctggag accagcctcg cgctggtcgc acacgtgctg ctgatcctga aagtgcccgc
    32221 gcaggaggtc atgcagacga ccgagaacat ccgccacgac cgctactcga tcctgcgcag
    32281 cgtgttccgc aagcggcgcg cgcgcagcct cggcggagac cacgccctgc gacagcaact
    32341 gcgcaccgtc gtgctgccgc ccggcgcgca ggcggtgggg cacacgattc gcgatgcggg
    32401 tctcgacgaa ggctcggtgc gcgtccgggc ggtgcggcgc gacggtatcg tcggccgcga
    32461 cccggatggc gacacacggc tgcgcgaagg cgacgtgctg gtcctgtggg gacccccgga
    32521 ggaactggat gtggcggaaa accggctcct gatgggctga cctggcggcg ggggccgcca
    32581 ccccaggtta ccgccccata acccgttttc tttccgatag ttagcgtcct cgaggtcgcg
    32641 caggcgtccc ggcaagcggc ccgccgtggc aggtcgactg aacgtcattg tgtacagtca
    32701 tgtagtaatt gacagtgtgt catcactcgc tcagcattgc cgcatgtcct acatcgaaga
    32761 acggcggcag gaagagcgcg accgacgccg cacggagatc gtcgacgccg cggacgcggt
    32821 gtacgcggaa tcgggctggg actcggtcac catggaccnn nnnnnnnnnn nnnnnnnnnn
    32881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    32941 nnnnnnnnnn nnnnnnnnga cgccgcggac gcggtgtacg cggaatcggg ctgggactcg
    33001 gtcaccatgg accaggtcgc gcggcgagcg cgcctgagcc gcgcgctcgt ctacgtctac
    33061 ttcaaggaca agccggacct gcacctcgcg ctggtcgagc gctcgctcga cacgctgcgc
    33121 ctgaggttcg aggcggcgcg cgccggcaag gcgaacgggc tcggggaact cgaggccatc
    33181 ggccgcgcct acgtggcgtt ttcccgcgag ctgccgcatt actacgatgc ctgcgcccgc
    33241 taccaggccc acgactgcgg cccggcgtgc gacggtggcg atctgcccgc gaatgaagcc
    33301 gcgtgcatgg ccgccggtcg acgcgtgcac gagaccatcg tggagtccat caggcgggga
    33361 atcgccgacg gatcggtgcg tgccgacatc ggcgatccga tgccgacggc gctggcactg
    33421 tgggcgttca cccacggcgt catccagatt gccgcgagca aggggggcca gatcgagcgc
    33481 gacggcgtgc cgatccagca gttcatcgac cacgcgttca gcctggcgct gcgctcgctg
    33541 cgcccgtgac cgaggcgatc gcaccgtgcg ccgcagtccc gccgtcctga aatcccgccg
    33601 tccctcggag atcggcgtcg cgctgccgtt gctcacctgg gcattgatgg cgggcacgac
    33661 gacgcccggc atcgcccgcg cggagacgac cctgcccgcc gttcccagcg cccggcaggt
    33721 cgcggctctg tcactcgcgt cggcggctgg gacccagatc caggacccac ggccggtgac
    33781 gtcggccggc acgaccgtcg aggtgggtct gcgcgaggtg ctcggcgagg cgctcgccgc
    33841 gaatctcgaa ctgcgtgccg gggccgccgg tgtcgcgcag cgcgttgccg cgctcgaccg
    33901 cgcccgcgcc cggtacctgc cggtcctcga tttcgatgca cgttactcgg tcgcagacgg
    33961 cgggcgcacc atcgactttc cggtaggcga cctgctcaat cccgtgtacg agacgctcga
    34021 ccagctgctc gtgcagtctg gccagccggc cgctttcccg agggtgaaga atgaggaaat
    34081 ccgcctgctg ctgccgcggg agcagaacac gcggctggtg ctggcacaac cggtctacga
    34141 gccgcgcctc gggcccgccg tcgagggcac gcggcaggaa ctcaaccgcg tcgaggcgga
    34201 ctactcgggc ctgcgctcgc gcgtgatccg cgacaccaag caggcgtact acgaatggct
    34261 cgtggcccag cagcaggtcc tggtgttcga cgccacgctg gaactcgcgc gctcgaacct
    34321 cgccgcgacc gagagcctgt accgcaacgg ccgcatcacc cgcgacctcg tctatcgagc
    34381 cgaggccgac ctgctcgaga tcgagcagca gcggctcgct gcagcgagtc gcgtgaccat
    34441 cgcgcagagc tacgtcaacc tgctgcgcaa ccagccgctc acctcgccgg tgccggcggc
    34501 gacgatcgac gccgcgaccg tcgagcgctt ccgcgatcga ctggtgcgcc gcgccgccgg
    34561 acgccgactc gacgcgcccg cactcgagga agtcgccacc ggccagcgcg cggaaatcca
    34621 gggcctcgac gctgcgatcg ccggcagcct cgcacagcag gacctcgccc gtgcggcttt
    34681 caagccagcg gtggccgtgg gtgccgaagc cggcatccag ggcgaggact acggcttcgg
    34741 cccggacgag cgctacgtgc tcgcgtccgt cgtgctgcgc tggaacacgt tccgcggcgg
    34801 cgccgacctc gcggcgctcg ccgaagcgcg cgccctcacc gacgagctgc gcgccacccg
    34861 cgacctcgcc acgcagcagg tgcggctgca ggtgcagcgc gcggtggagc aactcggcgt
    34921 cgccgaggcg tcgctcgcca cggcagagaa gcgcgccgtg gctgcggagg ccgcattccg
    34981 catcacggag cgcaagcgcg acctcggcca ggtgaaccag accgattaca tcgacgcgcg
    35041 caagacgctc accgacgccg agctcaacgt gaatcgcgtg cgcaccgagt tcctggcgcg
    35101 catcgcagaa ctggaattcg cgatcggcga ccgccgggcc gccgacgagg agccgcagtg
    35161 aatcgcattg ccgtcgccgt actcaccacc gccgcgctgg ccgtcgcagc ctgctcgcgc
    35221 gagacacctg cgccggaacc cgcgcacgcg tcggccaccc ccgtccgcgc ggcgccggtc
    35281 gagaccgtgc agctcgcggg gtcggtgcga gcggtgggca cgctcgagcc ggaatcggaa
    35341 gtccggctgt cgttcaaggt gggcggcgtg gtcgacagca tcaccgtcca tgccggcgac
    35401 caggtcaggc gcggccagct gctcgcgacc atcaagcgag cggaggtcga tacggccctg
    35461 gcgcaagccg ccgaggccga ggccaaggcg cagcgcgacc tcgaccgcgc ccgccgcctg
    35521 cgcgaggacg aagtcgcgac ggaggaacag gtgcaggacc tcgcgaccgc ctggaaggtg
    35581 gcacgcgcca acctggacgc cgcgcgcttc aatgcccgct actcgcgcat cgaggctccg
    35641 caggacggcg tcgttctgtc acgcctcgcg gaacaggacg agctggtgca agccggccag
    35701 ccggtgctcg tgctgggcgg cacgggcggc ggctggatcg tacggaccgc gctcgccgac
    35761 cgcgacgccg tgcgggtgga ggtgggcgac accgcgcgcg tcgagttcga cgcgttcccc
    35821 gggcgcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngag cgcgtggccc
    35941 gccgccgcca ggtcgaagtc ggtcccatcg tcggcgaccg tgtcgtggtg agcgcgggct
    36001 tgagcgacgg cgagcaggtc atcacggacg gcgcggcgtg gctcaccgac ggccagtccg
    36061 tcgagctggc cgcccaggcc ggctgaaccg catgcgcatc tgggccttca gcgtacgacg
    36121 ctggcagttc acgctgttgc tgttcgcgct gctcaccgcc atgggcgtga gctcgttcct
    36181 cggcatcccg cgctcggagg acccggaatt ccacgtgccg atcccgacca tcgtcgtcgt
    36241 ctacccgggc gcggaccccg tggacatcga gcggctggtc gtggatccca tcgaggacgc
    36301 gctgagcgag ctcgacgacg tcaagcgcat cgacagcgcc tccatggacg gcgtcggcgt
    36361 gatccgcccc gagttccact gggacaccga cgccgacgac aagtacgacg aggtggtgcg
    36421 cgaggtgagc cgcatccgcg gcgaactccc ggccgggatc acttcgctcg agatacgcaa
    36481 ggcgggatcg gggctcgtca acatcgtgca ggtcgcgctc gtgggcgccc aggtgccgga
    36541 ccgcgagttg aagaaactcg ccgaggacct gagcgactcg atcgagacgg tgcccggggt
    36601 tcgcagcagc gcctggtggg ccgtgcccga gcccgaggtc cgggtctcgg tggatctcga
    36661 gcgcatggga cgtgccggcg tcacgttgac gcaggtcgag caggccatcc gcggcggcaa
    36721 tgcgacggtg ccgggcggag cggtggacgt gggcctgcgc aagtacaacc tgaagacgtc
    36781 gggcggcttc gactcgctgc cggaaatttc cgacacggtc gtagcaagcc gcgacggccg
    36841 cgtggtccgc gtcgccgacg tcgcggccgt cagctgggcc agcgccgagg aaacctacgt
    36901 cggccgctac aagggcgagc gcgcggcgtt cgtcaccgcc aacgcaaagg acgccgtgaa
    36961 cgtcttcgcg gtccgcaatg ccatctacgc gaagctcggc gcgttcgaga aggaactgcc
    37021 cgcaggcgtg cgcctcgagc gcggcttcga ccagacgcgc aacgtcgcgc atcgactgaa
    37081 gcgcctggga atggatttcg ccatcgccat cgcgctggtg ctgctcacgc tgctgccgct
    37141 cggtctgcgg gcgtcggcgg tcgtgatgat ctcgatcccg ctgtcgctcg cgatcgggct
    37201 gtcgctgctc tactacacgg gcttctcgct gaaccagctg tccatcgcgg gcttcgtgct
    37261 cgcgctcggc ctgctggtcg acgactccat cgtggtcgtc gagaacatcg cccgcttcct
    37321 gcgcgaggga tattcgcgcg tggaagcggc aatcgcggct accgaccaga tctcgctcgc
    37381 cgtgctcggc tgcaccgcca cgctgctgtt cgcgttcctg ccgctgctgt tcctgccgga
    37441 gggcgccggc aagttcatcc gctcgctgcc ggcggccgtg ctgtacacgg tgctcgcctc
    37501 gctgttcgtc gcgctgaccg tgattccgtt cctcgcgagc cgcatgctgc gacccgaggg
    37561 ccacgacggc gaaggcaatc gggtgctgcg cgggctgatg tcggtcatca atcgtgtcta
    37621 cgcaccggcg ctgcgggtcg ccctcgcgtg gccgcgccgc acgctcctcg cggcgctcgc
    37681 gttcgtcatg gcttcgctcg cgatcgtgcc gttcatcggc gtcagcctgt ttccgcctgc
    37741 ggacatcccc cagttccgcg tcaccatcga gacgccggac ggtgcgagcc tcgccgacac
    37801 ggaccgcgcg ctcaggttcg tcgaggcgga actcggcagg caccccgagg tgaagcagtg
    37861 gttcgccaac ctcggccacg gcaatccccg cgtctactac aacatctttc ccgaggagac
    37921 gaaggccaac accgccgaag tattcgcgga actgcaccac ttcgacccgc ggcgcagccc
    37981 ggcgctgtac gaggagatcc gcgcgaagtt cgctcgctac ccgggcgcgc gcatcctgct
    38041 cgagtcctac cagaatggcc cgccgatcaa tgcgccgatc gagatctcgg tggccggccc
    38101 ggacctcggg cagttgcgtg aactcgctgc gcgcgtcgag aaggagatcg ccggcacgcc
    38161 gggcacgcgc gacgtggaca acccgttgcg ccgccagcgc accgacatcg acctgcgcat
    38221 cgacccggag aaggctgcac tgttcggcgt cgcaccggtg gacgcggacc gcacggtgcg
    38281 agccgcggtg gccgggctcc cggtcggcag ctatcgcgag tccgacggag acgagtacga
    38341 catcacgctg cggctgccga tgcagggccg ccagacactc gacgcgctcg gcctgatcga
    38401 ggtcgcttcc gcgtccggcc ggcccgtgcc gctgcgccag atctcgtcgc cgcagttcgc
    38461 cgccgcgccg agcacgatcg agcggcacga ccgggtgcgc gaggcgaaga tcacggccta
    38521 ccctgcggcc ggctacaaca ccgggaaggt gacgcgcgcc gtgatgcgtc gcctcgacgc
    38581 cttgggcctg ccgccggggt acagcctgcg cgtcggtggc gaactcgagg cgcgggagga
    38641 gagcttcggc ggcatgggcc ccgccgtgct cgtcgcagtg ttcgggatcc tcgccgtgct
    38701 ggtgctcgag ttcggcagct tccgctcgat gctgatcgtc gccggcgtga tcccgctcgg
    38761 ggtcgcgggc gggctcgtgg cgctgctgct cacgggttac acgctgtcgt tcacggcgat
    38821 gatcggattg atcgcgctca tcggcatcga gatcaagaac tcgatcctgc tggtcgattt
    38881 caccaaccag ctgcgcgcac agggagtcga ggtggacgaa gccatcaccc gcgccggcca
    38941 gatccgcttc ctgccgatcc tgctcacgtc cgcgaccgcg atcggcggcc tgctgccgct
    39001 cgccgtgcag ggctcgggac tctactcgcc cctggcaatc gtgatcatcg gtggactcgt
    39061 ctcgtcgacg ctcatcggcc gcctcgtcac cccggtgatg tacaaggtgc tgccgccagc
//
LOCUS       DAOMFD010000003        38984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_110_length_38984_cov_36.920368, whole genome shotgun sequence.
ACCESSION   DAOMFD010000003 DAOMFD010000000
VERSION     DAOMFD010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 38984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38984
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110_length_38984_cov_36.920368"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2525)
                     /gene="secA"
                     /locus_tag="PL152_00385"
     CDS             complement(<1..2525)
                     /gene="secA"
                     /locus_tag="PL152_00385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HQR47756.1"
                     /translation="MANWLTRIFGSRNQRLISQYDGIVRRINAFEPALQALTDAELAD
                     KTRQFREQLGNGKTLDDILPEAFAVVREASRRTLGLRHFDVQLIGGMVLHDGKISEMR
                     TGEGKTLVATLSAYLNALPGTGVHIVTVNEYLAGRDAEWMGPIYRFLGMTVGVVKAGQ
                     SADEKRAAYNADITYSTNNELGFDYLRDNLAFRLEDRMQRALTFAIVDEVDSILIDEA
                     RTPLIISGPAEESTELYTRINELVPRLRAQESEEGTGDFWADEKGKQVHLSDEGHEHV
                     EELFLKAGLLQPGQSLYDPANIRLMHHLNAALRAHALYRRDVEYIVRNGEVIIVDEFT
                     GRTMPGRRWSDGLHQAIEAKEGVRVREENQTVASITFQNYFRMYGKLAGMTGTADTEA
                     YEFQQIYGLEVVVIPTNVPMIRDDAADLVYLTQKDKFEAIIEDIRDCAQRRQPVLVGT
                     TSVETSEFLSDLLKKQGIEHQVLNAKQHEREALIVTQAGRPGTVTIATNMAGRGTDIV
                     LGGNLKAELAAVDGADEAALQAVRDDWQKRHEAVVAAGGLHIVGTERHESRRIDNQLR
                     GRSGRQGDPGSSRFYLSLEDNLMRIFGDPERTKRWLTTAGMKEGEVIESKMLSRQIER
                     AQRKVEAYNFDLRKNLLEYDDVANDQRKVIYQQRTELMAAEEISDSITGIREEVVNGI
                     IDQFVPRESVEEEWNLAGLSEAIARDFLVEIDPKAWLAADHEIAEAGLRERVVKAVED
                     AYQRKVEQVGKPVMVHLERVVMLQELDRHWREHLAAMDYLRQGIHLRAYAQKNPKQEY
                     KREAFELFGALLDRIKHDTISLLSRLQLRTEAEIEEQERERER"
     gene            complement(2650..3567)
                     /locus_tag="PL152_00390"
     CDS             complement(2650..3567)
                     /locus_tag="PL152_00390"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HQR47757.1"
                     /translation="MNFKIVSSRTGKVRHVDLKRPGVWLAAAAVSLTVVSLVFGAGLQ
                     VGRHIAGPLESDELARALDQQKLDLQTARGQLQGKVDALASRVGLMNAHLIRLDALGR
                     RITDLANLDRGEFNFDEPPPAGGPELGMQAAGSAQVPELASVLDSLEQQLADRERQLT
                     VLESLMSTRQLGARILPGGLPVIGGWISSHFGFRSDPITGHTAFHPGVDIAAVPGTHV
                     IATGPGVVSFAGFRSGYGNVVEITHPSGLVTLYGHNSRNLVREGESIQKGQAIAIIGS
                     TGRSTGTHVHFEVEKNGSPVNPAGYLGQP"
     gene            3801..4118
                     /locus_tag="PL152_00395"
     CDS             3801..4118
                     /locus_tag="PL152_00395"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HQR47758.1"
                     /translation="MAGVEKSRFGTPLEGLEHLARRAAAQDSLARLVQEMLPDPLGAH
                     VVGASRREADLVIIVDSAAWAARVRYAGPRLKEELAVRGEPVAGTIRVKVRGRAATSP
                     RGR"
     gene            complement(4133..5050)
                     /gene="lpxC"
                     /locus_tag="PL152_00400"
     CDS             complement(4133..5050)
                     /gene="lpxC"
                     /locus_tag="PL152_00400"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HQR47759.1"
                     /translation="MLKQRTLKNSIRATGVGLHTGKKVLMVLRPAPANHGIWFRRTDL
                     DEPVDIPARAEAVGDTTLGTTIVKDGVKVSTIEHLLSALAGLGIDNAIIELSSDEVPI
                     MDGSAGPFVFLLQSAGIEEQNAPKKFVRIRRSVMVEDGEKWARFDPHDGFRVNFEIEF
                     DHPVFKRRSQVASMEFSTTSFLREVSRARTFGFMRDLEYMRSRNLALGGNLDNAIVLD
                     DYRILNEDGLRYEDEFVKHKVLDAIGDLYLLGHGLIGEFSGYKSGHGLNNRLLRALIA
                     DSAAWEDVTFETLQDAPISYVAAASAAHA"
     gene            complement(5254..6426)
                     /gene="ftsZ"
                     /locus_tag="PL152_00405"
     CDS             complement(5254..6426)
                     /gene="ftsZ"
                     /locus_tag="PL152_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HQR47760.1"
                     /translation="MFVLADTENQGAVIKVVGVGGGGGNAVTHMVSSGIEGVDFVCIN
                     TDSQALKHSKVKMSLQIGQNITKGLGAGADPEVGRQAAMEDRDRIVELVEGSDMIFIT
                     AGMGGGTGTGAAPVVAQVAKELGILTVAVVTKPFEMEGNKRLALADQGIGELGKYVDS
                     LITIPNQKLLTVLGGDTTLLDAFKSANQVLQGAVQGIAELITRPGLINVDFADVRTVM
                     AETGMAMMGSGAATGENRARAAAEAAISSPLLEDVNLSGAHGLLINVTAGMDLRTSEF
                     HVVGDTIKQFASEDATVVVGYVIDPEMTDQIRVTVVATGIGRPAAIRGLPQGAQPKVE
                     VVAGGRAARRPLETYADYETPANLRRRQRAVGDDLRADPNAESFLDIPAFLRRQAD"
     gene            complement(6633..7868)
                     /gene="ftsA"
                     /locus_tag="PL152_00410"
     CDS             complement(6633..7868)
                     /gene="ftsA"
                     /locus_tag="PL152_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HQR47761.1"
                     /translation="MPKKTERNMIVGLDIGTSKVVAIVGELGLDGTIEVVGLGTHPSR
                     GLKKGVVVNIDSTVQSIQRAVEEAELMAGCEIHSVFAGIAGSHVRSLNSHGIVAIRDK
                     EVMPGDVDRVIDAARAVAIPADQKILHILPQEFIIDSQEGIREPIGMSGVRLEAKVHI
                     VTGADSAAQNIVKCVQRCGLEVEDIVLEQLASSYAVLADDEKELGVCLVDVGGGTTDI
                     AVFSGGAIRHTAVIPIAGDQVTNDIAVSLRTPTHHAEEIKVRYACALSQLANPDETIE
                     VPSVGDRPARRLARQTLAEVVEPRYEELFQLVREELRRSGFDEMIAAGVVLTGGSAKM
                     EGVIDLAEEVFHAPVRLGLPQGVSGLVDVVRNPIHATGVGLLLFGRENYVRRGRTAPV
                     ANGMRSVVDRMRAWFQGNF"
     gene            complement(7861..8658)
                     /locus_tag="PL152_00415"
     CDS             complement(7861..8658)
                     /locus_tag="PL152_00415"
                     /inference="COORDINATES: protein motif:HMM:NF015739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HQR47762.1"
                     /translation="MLAFWKKRNRRQPRNGEQSLVARVNWRALGAAAGILAGLALVAL
                     LLVLALDRPVRRVLVEGAFQRVSPPEIESAVVEVVHGGLASVDLDLVRERIERIEWVD
                     HAVVQRRWPDAIRVTVTEQVAAARWNEAGLLNARGELFVRNARYVPPELPLLQGPDGS
                     EHRVAQLYLEAQGPLLEAGLRLTGVRLDERGAWELQLTNGVVVKLGRQAVNERLGQFI
                     RLASPLVSKRLAEIKYVDMRYTNGFSVGWNARSALAVAPQEAATPDA"
     gene            complement(8668..9633)
                     /locus_tag="PL152_00420"
     CDS             complement(8668..9633)
                     /locus_tag="PL152_00420"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HQR47763.1"
                     /translation="MTHASHRITDPRDFGRVAVVMGGTSSEREVSLDSGRNVLEALVR
                     RGVDAFAVDGIPGLVAELVAPAAGRRIDRVFNILHGHRGGGEDGILQGLLDAFGVPYT
                     GSGVLGSALSMDKIRTKQVWMALGLPTPGYVSLERGDDVVAAAHKLGLPVIVKPALEG
                     SSVGISRCFKEADLAAAVELAARYAGPMLVEQLIVGDEYTVAVLGTDALPSIRIVPAG
                     EYYDYHAKYVAEDTQYVCPGATGAVEQEFGSLALAAFFAVGCSGWGRVDVMRDRAGKP
                     WLLEVNTAPGMTSHSLVPKAARMRGIEFDELVWRVLETSCVARRD"
     gene            complement(9630..11051)
                     /gene="murC"
                     /locus_tag="PL152_00425"
     CDS             complement(9630..11051)
                     /gene="murC"
                     /locus_tag="PL152_00425"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010951291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HQR47764.1"
                     /translation="MRDRMRRIQTIHFVGIGGSGMSGIAEVLVNLGYTVQGSDLKQGE
                     ATRRLEKLGARVMIGHRAENVAGADVVVVSSAINAENPEVAQALAQRVPVVKRAEMLG
                     ELMRFRHSIAVAGTHGKTTTTSLVASVLAEGGEDPTFVIGGRLKSAAANARLGAGRYL
                     VAEADESDASFLHLQPVIAIVTNVDADHLGTHGGDFGRLKASFIEFLHNLPFYGLAIV
                     CSDDAEAASLIPQIARPYVTYGFDESADVRAMNVTRRGAQSVFDVRRPGRPLLNVSLN
                     LPGRHNVLNALAAICVATELEIDDAAIQRALAGFQGIDRRLQWLGDVQAPAGRVTLVD
                     DYGHHPTEIAATMEAARQGWPDRRIVLVFQPHRYTRTRDLLDEFARVLSDADALLLAE
                     VYPAGEAPIAGADSKALCRAIRSRGRVEPVLLHSLDELPQALAGVARDGDVVLTMGAG
                     SIGAAAQELPAALAEIATQGGRA"
     gene            complement(11048..12139)
                     /gene="murG"
                     /locus_tag="PL152_00430"
     CDS             complement(11048..12139)
                     /gene="murG"
                     /locus_tag="PL152_00430"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HQR47765.1"
                     /translation="MSRPTAMIMAGGTGGHVFPALATARVLQRRGYDIVWLGTQRGIE
                     ARLVPAAGIPVEWLSVGGLRGKGITTLLAAPFKLATALMQALRAVRRHKPRVVLGAGG
                     FVSGPGGVAAWLTRTPLVVHEQNAIAGLTNRLLARLADRVLEGFPGSFGKGVKAECVG
                     NPVRKEIADIAPPERRYAGREGRARLLVFGGSQGAARLNAVVPAAIAELPDWLRPDVL
                     HQTGVRGAADTAAAYGARGIEADVRAFIDDMASAYGWADLAVCRAGALTIAELTAAGV
                     PAVLVPFPSAVDDHQTRNAQHMVRAGAAVLVAEAGLTPLSLAAKLRELLEAGRPRLER
                     MACAARASAITDADERLADACVAVAGGAA"
     gene            complement(12136..13320)
                     /gene="ftsW"
                     /locus_tag="PL152_00435"
     CDS             complement(12136..13320)
                     /gene="ftsW"
                     /locus_tag="PL152_00435"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HQR47766.1"
                     /translation="MTSTTISYARSTGQPRRYALDAWVIGTVAALLLVGLVMVASASI
                     GVSARESGNPFYYFEHQLFYVGMGLVAALVAMAIPTRIWEKHSIVLLIGAFALLLLVL
                     VPGIGHEVNGSRRWARLGFMNFQVSELARVMLLTYVASYAVRRSEELCSDFKGFIKPV
                     VVLGAAAVLLLLEPDFGAATVLMATGLAVLFLAGARLHHLLVPVVVGVAGMALLAVTS
                     SYRMRRLTAFRNPWEDPFDSGFQLVQSLIAIGRGEWFGVGLGSSVQKLFYLPEAHTDF
                     VFAVLAEEFGFLGVCVVIGLFALLVGRALEISRRAADADLPFQSCLAASIGIWLGLQA
                     FVNIGVNMGLLPTKGLTLPLLSYGGSSMLVTLGVIGLLLRINHETQQAGRPAASREAR
                     RE"
     gene            complement(13317..14672)
                     /gene="murD"
                     /locus_tag="PL152_00440"
     CDS             complement(13317..14672)
                     /gene="murD"
                     /locus_tag="PL152_00440"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HQR47767.1"
                     /translation="MTAHATSLAPLEPGSRALVLGLGRTGLSVARFLKRKGLRVRVAD
                     TRAEPPGVAKLLSDVPEAEVRTGAFASGLLDDVAQVVISPGLSVKEPVAREAQQRGLP
                     LVGDIELFAREAQAPVAAITGTNGKSTVTALLAELATAAGQPAVAGANLGEPALDLLE
                     RPVPALYVLELSSFQLETTYSLATATATVLNVTPDHMDRYDTLADYAEAKARIFDGCE
                     VAVVNADDPLVREMPRPGQRVLSFGLMDEAADYTLADGAQPTLACRGEPLLPVSALRI
                     KGLHNVANALAALAMCEALGLPRAPVLEALAGFGGLAHRAQWVRDVAGVHFVNDSKGT
                     NVGATIAAVTGTSGPVVLIAGGDGKGQDFGDLRAAFAGKVRHAVLIGRDGPRLAAVLE
                     GVCTTESAADMASAVAAARAVARPGDTVLLSPACASLDMFRDYAHRGDEFATAVRSLA
                     A"
     gene            complement(14669..15751)
                     /gene="mraY"
                     /locus_tag="PL152_00445"
     CDS             complement(14669..15751)
                     /gene="mraY"
                     /locus_tag="PL152_00445"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005297383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HQR47768.1"
                     /translation="MLRHVAEWLAQYYSGFRVFNYLTFRAILAALTALTISFMVGPGM
                     IRRLAERQVGQRVRSDGPQSHLSKAGTPTMGGALILVAIVVGTLLWADLANRLVWIVL
                     GVTVAFGLIGFWDDYLKLVVGNSRGLIARYKYLWQTVVGLGAAIALYLTARTAAETTL
                     IVPFVKSFVLPLGPLFIVLAYFVIVGTSNAVNLTDGLDGLAIMPAVMVAGALGVFAYA
                     SGNVVFANYLGIPYIAGAGEVLVICAAIFGAGLGFLWFNTYPAQVFMGDIGALALGAA
                     LGVIAVIVRQEIVLFIMGGVFVMETVSVILQVGSFKLRGKRIFRMAPIHHHYELKGWA
                     EPKVIVRFWIITVILVLIGLATLKLR"
     gene            complement(15753..17132)
                     /gene="murF"
                     /locus_tag="PL152_00450"
     CDS             complement(15753..17132)
                     /gene="murF"
                     /locus_tag="PL152_00450"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HQR47769.1"
                     /translation="MIRDLAEFARVAGGRLDGANAAFGAVTTDSRAVERGELFVALRG
                     ERFDAHDFVRQAAERGCAGAVVSRKVKAAVPQVVVADTLEALTAFARAWRHDFSGTVV
                     GLTGSNGKTTVKEMIGAILGRCGPCLVTQGNLNNHIGVPLTLARLEAGHRYAVIEMGA
                     NHRGEIAHLASIARPDVGLVINAGPAHLEGFGGIEGVARGKGEMFEALGVECAAVVNA
                     DDRYAAFWHGLAREAGRIVTFGVREHADFMAGGVECRLADDGFVTDFDLTCPLGRSRV
                     SIALAGEHNVMNALAAAATAAAAGASLDAITAGLGSVRPVAGRLQLRAALNGARLVDD
                     SYNANPGSVRAGLKALATVPGEHWLVLGEMRELGEDSARLHEEIGDLARTSGIRRLFA
                     VGEDARRAVEAYGAGGLWFADVEELVAAARPHLRSGVTVLVKGSRSNRLERVADALAA
                     EGAPRRGGH"
     gene            complement(17129..18643)
                     /locus_tag="PL152_00455"
     CDS             complement(17129..18643)
                     /locus_tag="PL152_00455"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HQR47770.1"
                     /translation="MVESELPPVYRASLATLLGDDVRLPRDLTVAGLTQDSRGATQGS
                     AFLACRGRSTHGLAHAADAVARGAVAVLWEPAAGVDVPALPGHVVVQAVPDLAQRVGT
                     IADRFFRAPSADLAVAGITGTNGKTTTAYLLAQAADAVGRRGAYLGTIGYGRPGALVD
                     AGLTTPDCVNVHRRLAQARDAGAKTMGIEVSSHALDQGRVDGVRFDTAVFTNLTRDHL
                     DYHGSLEAYGAAKARLFATPGLRGAVVNVRDEFGRALADRLDASIERVFYSSSNEVWA
                     PAGSAWIRLPELRATTAGLTLHVESSWGAGTLRSRLVGEFNADNLLAVLGVLLGWKVP
                     LQQALAALATCAPPPGRMEAFGGGAQPLALVDYAHSPDALAKVLDAARAHARGRLYCV
                     FGCGGDRDPGKRPIMGSIAEQRADVVVVTDDNPRTEDSRSIIEQILGGMREPDAAHVI
                     ADRAEAIHHALAEADAGDVVVIAGKGHEDYQVVGRDVRPFSDRKVVLDALGSEA"
     gene            complement(18673..20403)
                     /locus_tag="PL152_00460"
     CDS             complement(18673..20403)
                     /locus_tag="PL152_00460"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HQR47771.1"
                     /translation="MIRDSKRDRSPGRQPWRHWLVLGALAAAGLTLLARSVYLQVVDQ
                     AFLEQQGDARILRKATLSANRGMILDRNGEALAVSTPVDTVWADPRKLAEVPQEFPRL
                     AKALDRDPQWLARRVTSSLDREFIYLVRHMRPADAAKVRALGIPGVDTLREYRRYYPA
                     GEVTGHLLGFTNVDDVGQEGLELAYDQWLGGVPGEKIVMRDSLGRTIEDIEQLSAPRP
                     GQDLRTSIDLRVQYLAYRELKAAVQANRAIAGSIVVLDIATGEVLAMVNQPAFNPNDR
                     EQYLPSRYRNRATNDFLEPGSSIKPFVVAAGMETGRFHADTLIDTSPGIMRVGIKTVK
                     DHNNLGTIDVTTVLAKSSNVGAAKIALTLKPQEMWGVYDAFGFGRVTGSGFPGESAGI
                     LPGYEHWRAISQATMAYGYGLSVTPLQLAQAYAVLGAGGIRRPVSLRRVDTPPAGERV
                     MPEPVARELVRMLQSVVTDAGTARRAAIMGYQVSGKTGTAWKASETGGYSTDKYRAIF
                     GGVVPASNPRLAAVVIIDEPSAGAYYGGEVAAPVFAAVMSGALRVLAIPPDDLQGIPA
                     TTLVRAGDPW"
     gene            complement(20400..20675)
                     /gene="ftsL"
                     /locus_tag="PL152_00465"
     CDS             complement(20400..20675)
                     /gene="ftsL"
                     /locus_tag="PL152_00465"
                     /inference="COORDINATES: protein motif:HMM:NF016860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HQR47772.1"
                     /translation="MSTVAWRCAVSLLGLALLASALGVIFAKHEARSRFYELQRLTKE
                     RDDLDIEWGQLQLEQSTWATNGRVESVARGDLHMVIPQATDLRIVQP"
     gene            complement(20672..21607)
                     /gene="rsmH"
                     /locus_tag="PL152_00470"
     CDS             complement(20672..21607)
                     /gene="rsmH"
                     /locus_tag="PL152_00470"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HQR47773.1"
                     /translation="MAEHTPVLPDEVLAGLAVRADGRYCDATFGRGGHTAAILRALGP
                     QGRVIAIDRDPEAIRSGAARFQGEPRLALVKGSFGRLEELVRATGLEGELNGVLMDLG
                     VSSPQLDEAKRGFSFMQDGPLDMRMDNEAGQSAAQWIARADEREIAAVVRSLGEDRYA
                     NRIARAIVAARAERPIERTGQLAGIVAAAVPTREPGKHPATRTFQAIRIHLNGELEAL
                     EAALPQAVRLLAPGGRLCVISFHSLEDRIVKRYIRREEQGDPVYAGLPDAQVPPHARP
                     RLKRVGKAIEAGEAEVARNPRARSAVLRVAERLAA"
     gene            complement(21616..22071)
                     /gene="mraZ"
                     /locus_tag="PL152_00475"
     CDS             complement(21616..22071)
                     /gene="mraZ"
                     /locus_tag="PL152_00475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HQR47774.1"
                     /translation="MFRGANKVTLDAKGRMAMPARYRDTLVERSEGRLVATVDRADRC
                     LLIYPLPDWEEIERKLMRLPSLNPQARRLQRLMVGHATDLELDSHGRVLVPPKLREYA
                     GLTRQATLIGQGNRFELWDEQHWNARREAWLQAGEETGELPPELEQLSL"
     gene            complement(22549..22920)
                     /gene="rnpB"
                     /locus_tag="PL152_00480"
     ncRNA           complement(22549..22920)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PL152_00480"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(22957..23844)
                     /gene="rsmI"
                     /locus_tag="PL152_00485"
     CDS             complement(22957..23844)
                     /gene="rsmI"
                     /locus_tag="PL152_00485"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HQR47775.1"
                     /translation="MAKEPVDSIAGPRHGALFLVATPIGNLGDLSPRARDLLRDADLL
                     LAEDTRHTARLLHACGIERPPGSVESLHEHNERSRVPRVLERLRAGARVALVSDAGTP
                     LVSDPGAPLVRAAIDAGVEVVAVPGPCAAIAALSTSGLPAERFAFEGFLPARAAARRR
                     ALESLATEPRTLVFYEAPHRLEEALADLAQCLGGERTAAIAREITKRFESAYRGTLAD
                     LARRAALEPDMARGEIVIVVHGAAQSSDEDSRDSDRILRALLAEVTVSQAARLAATIT
                     GRPRKELYERALAIAQREE"
     gene            23866..25734
                     /locus_tag="PL152_00490"
     CDS             23866..25734
                     /locus_tag="PL152_00490"
                     /inference="COORDINATES: protein motif:HMM:NF016251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HQR47776.1"
                     /translation="MLHASLRGRQVRALLLTVALAAGAAQLPAPARADAGEQLSQAAA
                     LSRDGQHEAAARIYEQQARRVFRAWDARLSLLAAREYLEAGRTSDAARVLSKVTGPLR
                     GDDIVLYGRVKAELALARGDGPAALGALAAIPRPWPAPLAQELLLLEGRADFLAGRTL
                     EGIRAFEERGQVLGGAEARAENYRLLVDELGRSDAVLAVPAGATDGERAWIELAQIRA
                     MSQGDAAVVAQRAADWRTRHPNHPGAAFLPQPASNAGGVAVPVAAPGQGSATVALLLP
                     LSGRQQAVGVAVRDGFIAASLAHDDTGRKVLVYDTAALGPAQAYQRALADGAGAVVGP
                     LTREDVAALSGSQPLAVPTLALNAYAGAGAPPAFLYEFTLDPEQEARAAARRIAADGR
                     THGIALFPRSPWGERIYAAFTAELQQAGVSLTSAQYYEPGSRDFSGPLRAALGRYGGA
                     ADRSDGRPPPKRDPVAEARDGPQFAFIAATASTMRTIVPQLRFQMTYALPVYATSDAW
                     DPVPRAVPDLDGMVYPEFPWVLQGGEGARGLWEVLQHEWAANARGRVRLYAFGHDAAT
                     VAAAITSGHADSPIDGLTGRLALGGDARVHRELDWAQIVDGRPQPAAVTVSTAPPR"
     gene            25731..26105
                     /locus_tag="PL152_00495"
     CDS             25731..26105
                     /locus_tag="PL152_00495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HQR47777.1"
                     /translation="MTTETTTIVGRRGEDAALEHLAAAGLRLLERNYRCRGGEIDLVM
                     ADGTTLVLVEVRLRSSREFGGAAASVDRRKQRRFTLAARHLVLTHPEFRSMPARFDVV
                     ALERGDGAGEMRIEWLRDAFRM"
     gene            complement(26146..26562)
                     /locus_tag="PL152_00500"
     CDS             complement(26146..26562)
                     /locus_tag="PL152_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HQR47778.1"
                     /translation="MKSRRPYLLRAMHEWICDSECTPHIVVDAGVAGVEVPRQYVRDG
                     KIILNVSWNATAQLRLGNDAVSFSGRFGGVSMVVRVPIEAVLAIYARETGQGMIFSDD
                     EGGPAPAPPEGPTPEGAPAEATSKPAPGRAKLKVVK"
     gene            complement(26559..27155)
                     /locus_tag="PL152_00505"
     CDS             complement(26559..27155)
                     /locus_tag="PL152_00505"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQR47779.1"
                     /translation="MTLFSRGDDPRSHRVRIVVAAKGLEARIVEVDPARPPEDLIDLN
                     PYQSVPTLVDRDLVVYEAGIICEYLDERFPAPALLPVDPAARAQSRVALHRIEQDWYA
                     LAPALESADRREQQKGRRLLLESVVASEPLFRLRPWFLSDQFSQLDAAAAPILWRLAR
                     WEIGLPADAQSIERYARRVFAHPAFRASLSRIEQEMRA"
     gene            complement(27262..28002)
                     /locus_tag="PL152_00510"
     CDS             complement(27262..28002)
                     /locus_tag="PL152_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009174980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c1"
                     /protein_id="HQR47780.1"
                     /translation="MRTKLLVLAAALAPLAAFAAGAEAQLDRFNADVSNQASLQRGAR
                     NFVNYCLGCHSAKYVRYNRIAADLGLTEQQVIDNLMFTGDSVQGTMRIGINPDDAKRW
                     FGTVPPDLSLIARARGTDYLYTFLRSFYADPTRPTGVNNIVLPGTAMPHVLWKLQGVQ
                     EAVWVGHPDAQGNVQKHFDKFQPGTPGTMTAEEFDGFVRDTVNFLDYIGEPAQLQRTR
                     LGYPVIAFLLFFTLLAWALKKEYWKDVK"
     gene            complement(27992..29257)
                     /locus_tag="PL152_00515"
     CDS             complement(27992..29257)
                     /locus_tag="PL152_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bc complex cytochrome b subunit"
                     /protein_id="HQR47781.1"
                     /translation="MGSTNQTPAPAGTGRLASLVAWIDARFPMTKLWNESAAEYYAPK
                     NLNFWYYFGSLAMLVLVIQILTGIFLTMNYKPAAADAFGSVEYIMRDVDWGWLIRYMH
                     STGASAFFIVVYLHMFRAVLYGSYQKPRELLWIFGMVIYFLLMGEAFFGYLLPWGNMS
                     YWGAQVIVSLFGAIPYVGDSLTEWIRGDFYISDVTLNRFFALHVVALPLALVFLVIAH
                     LIALHEVGSNNPDGVEIKSQKGADGHPLDGIRFHPYYTVKDMVGVGVFLILFCLVIFF
                     APTMGGYFLEHANFEPANALQTPPHIAPVWYFTPFYAILRSVPNKPLGAALMGLAVLL
                     FVFLPWLDRGKVKSIRQRGPIFKFFVAAFAISFIALGYLGMQPPTPTYAIAGRVFAAV
                     YFAFFLLMPWYTSIDKTRPVPTRVTYHAH"
     gene            complement(29257..29847)
                     /gene="petA"
                     /locus_tag="PL152_00520"
     CDS             complement(29257..29847)
                     /gene="petA"
                     /locus_tag="PL152_00520"
                     /EC_number="7.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045275 - respiratory chain complex III
                     [Evidence IEA];
                     GO_function: GO:0008121 - ubiquinol-cytochrome-c reductase
                     activity [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HQR47782.1"
                     /translation="MTEEVDTGRRHFLTVATIATGAVGAAFAAVPFLASWKPSARAKA
                     MGAPVEVDVGKLEPGAMVKVEWRGKAIYVVHRSPQLLAEIKGHDARLRDPDSKESEQP
                     KFAQNANRSLKPEYLVLVGVCTHLGCAPLDKFQAGDPTVSADWPGGFFCPCHGSKFDM
                     SGRVFKDVPAPTNLPVPPYRFVSDTRILIGAESGNA"
     gene            complement(30016..31284)
                     /gene="murA"
                     /locus_tag="PL152_00525"
     CDS             complement(30016..31284)
                     /gene="murA"
                     /locus_tag="PL152_00525"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HQR47783.1"
                     /translation="MDKLQIRGGVPLDGEVRISGAKNATLPILAGALLADGPVTIGNV
                     PHLQDVTTMIELLGRMGASVTVDERMRIEVDPRTTRETFAPYELVKTMRAAILVLGPL
                     VARHGSADVSLPGGCAIGARPVNIHVAGLQAMGADVHIENGYIKARAGRLKGARIVLE
                     TVTVTGTENLMMAACLAEGRTILENAAREPEIVDLAQFLNSMGARVSGHGTDTIVIDG
                     VERLHPTSYDVLPDRIEAGTYLVAGAITGGRVRARGARAEHLDAVLAKLEEAGATVTR
                     GEGFVEVDMRGRRPRAVDIRTAPHPGFPTDMQAQFAALNTVAEGVGTIVETIFENRFM
                     HMLEMRRMGAEIRLEGNTAIIRGVPRLTAAPVMATDLRASASLVLAGLVADGTTEVER
                     IYHIDRGYECIEEKLHGLGAQIRRIGRARK"
     gene            complement(31298..31555)
                     /locus_tag="PL152_00530"
     CDS             complement(31298..31555)
                     /locus_tag="PL152_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012510288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA/IbaG family iron-sulfur metabolism protein"
                     /protein_id="HQR47784.1"
                     /translation="MTADEVTRLIESGLSGAQVRVLTDDDTHFEAVIVAAQFDGKRSI
                     QRHQLVYAALGPRMGREIHALSIQAYTPAEWQAARGSPGPG"
     gene            31716..32690
                     /locus_tag="PL152_00535"
     CDS             31716..32690
                     /locus_tag="PL152_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HQR47785.1"
                     /translation="MPAPDPSIARGRQVLETEAGAVAALASRLGDEFARACGLLLACK
                     GRVVVTGMGKSGHVGGKIAATLASTGSPSFFLHPAEASHGDIGMITAGDVVIALSNSG
                     ETGELLTILPLIKRLGTPLIALTGNRNSTLGRYATVVLDVSVPAEACPLNLAPTASTT
                     AALAMGDALAVAVLEARGFTEEDFARSHPAGSLGRRLLLLVEEVMRRGDDLPAVGPDT
                     LLSAGLLEMSRKGLGMTTIVDSSRRVLGIFTDGDLRRALDRQIDVHATPMREVMTAGG
                     KVAKPRMLAAEAVRMMQDHRITALPVVDDAGILVGALNVHDLLRAGVM"
     gene            32693..33187
                     /locus_tag="PL152_00540"
     CDS             32693..33187
                     /locus_tag="PL152_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIIA family hydrolase"
                     /protein_id="HQR47786.1"
                     /translation="MDAVLDRARRVRLLVLDVDGVLTDGRLYIAASGEEFKVFHVRDG
                     AGIVAVQRAGIAVAIISGRNSAAVSRRAEELGIRHVRQGVADKAAALRTLLGELGVAA
                     AETACVGDDTPDLPMLAAAGLAIAVADAHPEVRSAAHWVTRVNGGCGAVRETCDLLLS
                     AATS"
     gene            33154..33729
                     /gene="lptC"
                     /locus_tag="PL152_00545"
     CDS             33154..33729
                     /gene="lptC"
                     /locus_tag="PL152_00545"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HQR47787.1"
                     /translation="MRSPAQRRYVLTQRLVAVAAVTAALALLYGLFGPEPPDVEKGAT
                     PPQQGYYLVDTTMTEMGPTGRPKVIVHARHAEQQLADQSVDASDITLDSPTDKYGTWH
                     GTALRGHLTPDRNTIQLAGDVTMTSQLEQGVAVIHTEHLDYDVDDSVITTSDAVDVRL
                     GAHVLKARGLRADMNAQTLRLESDVNGRFTP"
     gene            33710..34405
                     /gene="lptA"
                     /locus_tag="PL152_00550"
     CDS             33710..34405
                     /gene="lptA"
                     /locus_tag="PL152_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR03002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0001530 - lipopolysaccharide binding
                     [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide transport periplasmic protein
                     LptA"
                     /protein_id="HQR47788.1"
                     /translation="MAASLPDVARVRIAVALLCCAAAITASGAVGAAESIELPRPSKD
                     LPIDLVAASSDVDYKNNALRFRQLVVTQGPLRVEAQEATATGLDFTKSDWTLTGQVRI
                     TMPDGKLTADDAQLAFRNNEIARATAHGAPAEFEHKLKDSPQVVKGHADTIDYNVQAG
                     TVRLSGAGWLTDGDNVIRGDTLVYGLATQRVQANPDSAVPGGVHITINPKSQDKPGGK
                     TTDQPKGKPDAHP"
     gene            34402..35130
                     /gene="lptB"
                     /locus_tag="PL152_00555"
     CDS             34402..35130
                     /gene="lptB"
                     /locus_tag="PL152_00555"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004091254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HQR47789.1"
                     /translation="MSVLRAESLGKRYRSRKVVSGLSLDVSSGEVVGLLGPNGAGKTT
                     AFYMIVGLIPCDEGRIHLDDRELTRLPMYRRARLGVGYLPQEASVFRKLSVEDNIRAI
                     LEVREDLDDEAAREAALDALLEELHIGHIRTNVGMSLSGGERRRVEIARALAAEPRFI
                     LLDEPFAGVDPISVDDIQRIIRHLAARNIGVLITDHNVRETLGICNRAYIVSAGSVIA
                     SGSAQEILANPQVREVYLGQDFKL"
     gene            35136..36578
                     /locus_tag="PL152_00560"
     CDS             35136..36578
                     /locus_tag="PL152_00560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF009118.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HQR47790.1"
                     /translation="MLKPALQLKLGQQLTMTPQLQQAIRLLQLPVLELQAQVQEALES
                     NVMLEVEEEEEPIDLASLDQPAPAGEEGAESDTGSEPADEVVVEMEDPWSEVSAAPGE
                     GRSQDDDDRPLEFTDNRELNLHQHLVWQLEVSKLDPREVWIGEALVDALNDDGYLTES
                     TADIARSLSADLPVTEADVERVLAFLQTLDPAGVGARSVAECVCLQLGQLDAGTAGRE
                     LALRIAAQHLQEVANRDLPGLRQRLGVAEDEFHEALALIRACNPRPGAAFESAPPEYI
                     VPDVFVRRTQHGWSVELNPASVPRLKVNQGYASLVSRSQDYATLRAQLQEARWLIRSL
                     EIRGETLLKVARSIVQRQAAFLEQGEEAMQPMILRDVAEAVGMHESTISRVTTGKYMH
                     TPRGIFEFRFFFSSHVSGAGGEDVSSVAIRARIRKLIADEQPGKPLSDAQLADVLARE
                     GVKVARRTVAKYREALGLASSSERRHAAAG"
     gene            36580..37452
                     /gene="rapZ"
                     /locus_tag="PL152_00565"
     CDS             36580..37452
                     /gene="rapZ"
                     /locus_tag="PL152_00565"
                     /inference="COORDINATES: protein motif:HMM:NF015620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HQR47791.1"
                     /translation="MRLIIVSGLSGSGKSVALNMLEDLGYYCVDNIPAGLLPAFIAYT
                     VRTSEPSYRLTAVGVDARNRPEDLADVPRLVAELRRSGIGCELLFLRAGNDVLLKRFS
                     ETRRRHPLSREGLGLQEALEQEQRLLAPVATAADLTVDTSSLSMHELRDMIRERVVER
                     PAAGPSLLFESFAYRNGVPDDADFVFDARSLPNPYWEPALRDFTGRDEPVRQFLSRQE
                     EVARFQADVTAFLERWMPSLARSNRSYLTIAVGCTGGQHRSVYLAEQLAAHFNASLGQ
                     ALVRHRDLAGRRHE"
     gene            37805..>38984
                     /gene="mgtE"
                     /locus_tag="PL152_00570"
     CDS             37805..>38984
                     /gene="mgtE"
                     /locus_tag="PL152_00570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQR47792.1"
                     /translation="MNAELHPMRMSRLATLKRALEQGTGRQVQRLVNSLHPAEVASLL
                     ESLPHAQRDLVWDLVDPELEGDVLVELNDEVRADLIQEMGTEELVAATEGLEVDDLAD
                     LLADLPETVNQQVLRSMDSQDRERLKAVLAYDADTAGGLMNTDTVSVRPDVTLETVLR
                     YVRMRGELPERTDSLYVVNRHDRYLGTLALPRILTEDPDRTVGEVMDAAAEAIAPETP
                     ASQVASLFQDHDLLSAPVVGPEGRLLGRITVDDVVDVIRDEADHSVMSMAGLDEDADM
                     FAGVVPSARRRGVWLGINLATAFLAAWVVGLFESTIQSIVALAVLMPIVASMGGVAAT
                     QTLTLIVRGLALGQVERANARWLMAKEIAVALLNGLAWATVVAVVALAWFRNWKIAAV
                     I"
BASE COUNT         5754 a        13840 c        13566 g         5824 t
ORIGIN      
        1 tggcgctcac gctcgcgctc ctgctcctcg atctcggcct cggtcctgag ctgcaaccgc
       61 gacagcagcg agatcgtgtc gtgcttgatc cggtcgagca gcgcgccgaa cagttcgaag
      121 gcctcgcgct tgtactcctg cttcgggttc ttctgcgcgt aggcgcgcag gtggatgccc
      181 tgccgcaggt agtccatcgc cgcgaggtgc tcgcgccagt gccggtcgag ctcctgcagc
      241 atcacgacgc gctcgaggtg caccatgacc ggcttgccga cctgctcgac cttcctctgg
      301 taggcatcct ccacggcctt cacgacgcgc tcgcgcagcc cggcctccgc gatctcgtgg
      361 tcggcggcga gccacgcctt cgggtcgatc tcgaccagga aatcccgcgc aatggcttcg
      421 gacagccccg cgaggttcca ctcctcctcg acgctctcgc gcgggacgaa ctggtcgatg
      481 atgccgttga cgacctcctc gcggatgccg gtgatggagt cggagatctc ctccgccgcc
      541 atcagctcgg tgcgctgctg gtagatcacc ttgcgctggt cgttcgcgac gtcgtcgtac
      601 tcgagcaggt tcttgcgcag gtcgaagttg taggcctcga ccttgcgctg tgcgcgctcg
      661 atctggcgcg agagcatctt gctctcgatc acctcgcctt ccttcatgcc ggcggtcgtg
      721 agccagcgct tggtgcgctc cgggtcgccg aagatccgca tcaggttgtc ttcgagcgac
      781 aggtagaagc ggctcgagcc cgggtcgccc tggcggcccg agcggccgcg cagctggttg
      841 tcgatgcggc gcgactcgtg gcgctcggtg ccgacgatgt gcaggccgcc cgcggccacg
      901 actgcctcgt ggcgcttctg ccagtcgtcg cgcaccgcct gcagtgccgc ctcgtccgcg
      961 ccgtcgaccg cggcgagttc cgccttgaga ttgccgccga gcacgatgtc ggtgccgcgg
     1021 ccggccatgt tggtggcgat ggtgacggtg cccggccgcc cggcctgcgt cacgatcagc
     1081 gcctcgcgct cgtgctgctt ggcgttcagc acctggtgct cgatgccctg cttcttcagc
     1141 aggtcggaga ggaactccga ggtctcgacc gaggtcgtgc cgacgagcac cggctggcgg
     1201 cgctgcgcgc agtcgcggat gtcctcgatg atcgcctcga acttgtcctt ctgcgtgagg
     1261 tagacgaggt cggccgcgtc gtcgcggatc atcggcacgt tggtcgggat cacgaccacc
     1321 tcgagcccgt agatctgctg gaactcgtag gcctcggtgt ccgccgtgcc ggtcatgccg
     1381 gcgagcttgc cgtacatgcg gaagtagttc tggaaggtga tcgaggcgac cgtctggttc
     1441 tcctcgcgca cgcgcacgcc ttccttggcc tcgatcgcct ggtgcaggcc gtcggaccag
     1501 cggcggcccg gcatggtgcg gccggtgaac tcgtcgacga tgatcacctc gccgttgcgc
     1561 acgatgtact cgacgtcgcg gcggtagagc gcgtgcgccc tgagcgccgc gttcaggtgg
     1621 tgcatcaggc ggatgttggc cgggtcgtac aggctctgtc ccggctgcag cagccccgcc
     1681 ttgaggaaca gttcctcgac gtgctcgtgc ccctcgtcgg acaggtgcac ctgcttgccc
     1741 ttctcgtcgg cccagaagtc gcccgtgccc tcctccgatt cctgtgccct gagccgcggc
     1801 acgagctcgt tgatgcgcgt gtagagctcg gtgctctcct cggcgggacc cgagatgatg
     1861 agcggcgtgc gggcctcgtc gatcaggatc gagtcgacct cgtcgacgat cgcgaacgtg
     1921 agggcgcgct gcatgcggtc ctcgagccgg aacgcgaggt tgtcgcgcag gtagtcgaag
     1981 ccgagctcgt tgttggtgct gtaggtgatg tcggcgttgt aggcggcgcg cttctcgtcg
     2041 gccgactgcc ccgccttcac caccccgacc gtcatgccga ggaagcggta gatgggcccc
     2101 atccactcgg cgtcgcggcc cgcgaggtac tcgttgaccg tgacgatgtg cacgccggtg
     2161 cccggcagcg cattcaggta cgcggacagc gtggcgacca gcgtcttgcc ctcgccggtg
     2221 cgcatctcgg agatcttgcc gtcgtgcagc accatgccgc cgatcagctg cacgtcgaag
     2281 tggcgcagcc cgagcgtgcg gcgcgacgcc tcgcgaacga ccgcgaacgc ctcgggcagg
     2341 atgtcgtcga gggtcttccc gttgcccagc tgctcgcgga actggcgggt cttgtcggcc
     2401 agctcggcat cggtcagcgc ctggagcgcg ggctcgaagg cgttgatcct gcggacgatg
     2461 ccgtcgtact ggctgatcag ccgctggttg cggctgccaa agatgcgggt gagccaattg
     2521 gccactgggg ctccgggaga caggggcagg ggaaagggcg aaattttaca ggaaagaggc
     2581 ttccgggtac cggcccgggg ccccggcccg cgcccgtatg acccgttcgt tggccccggc
     2641 tggccgaatt caaggctggc cgaggtatcc cgccggattc accgggctgc cgttcttctc
     2701 gacctcgaag tgcacgtggg tgcccgtcga gcgcccggtg ctgccgatga tggcgatcgc
     2761 ctgtcccttc tgtatcgact cgccctcgcg cacgaggttg cgggaattgt gcccgtacag
     2821 cgtcacgagg cccgacgggt gggtgatttc caccacgttg ccgtacccgg agcgaaatcc
     2881 ggcgaaactg accacgccgg gtccggtcgc gatcacgtgg gtgcctggca cggccgcgat
     2941 gtcgacgccc ggatggaacg ccgtgtgtcc cgtgatggga tcggagcgga agccgaaatg
     3001 cgacgagatc cagccgccga tcacgggcag gccgcccggc aggatgcgcg cgccgagctg
     3061 gcgcgtggac atgaggctct cgagcaccgt gagctggcgc tcgcggtccg cgagttgctg
     3121 ctccagcgaa tcgaggacgc tcgccagctc cggaacctgc gccgagccgg ccgcctgcat
     3181 gccgagttcc ggtccgcccg ccggaggcgg ctcgtcgaaa ttgaactcgc cgcggtcgag
     3241 gttcgcgagg tcggtgattc gccggccgag cgcgtcgagg cggatcaggt gcgcgttcat
     3301 caggccgacg cgcgatgcga gggcatcgac cttgccctgc agctggccgc gcgccgtctg
     3361 caggtcgagc ttctgctggt cgagtgcacg cgcgagctca tcgctctcga gcggacccgc
     3421 gatgtggcga ccgacctgga ggccggcgcc gaagacgagc gacacgacgg tcagcgacac
     3481 ggccgcagcc gcgagccaca cgcccggccg cttcaggtcg acgtggcgca ccttgccggt
     3541 gcgactcgaa acaatcttga aattcatcgc ggcgcttttc ccctcggcag ccgtggaacc
     3601 ggccgtcgat cgtcaatgcg acggccttgc aacgcttcga cccctggcgt gccttttcgc
     3661 tggctagaca cgcaccaacc gccagtaccc agctggaggg agaccggcca atggaccagc
     3721 ccggtcaagg gtgcgagact atgaacgaag cgccctgtgc ccgcaataag tgagtgccgt
     3781 ctgttaacga aaaattgact atggcaggcg tggaaaagtc tcgcttcggc accccgctcg
     3841 agggcctcga gcacctcgca cgacgggctg ccgcccagga ctcactggcg aggctcgtgc
     3901 aggagatgct gccggatccg ctgggcgcgc acgtcgtggg ggccagccgc cgcgaggcgg
     3961 acctggtgat catcgtcgat tcggccgcct gggcggcgcg cgtgcgctat gcgggcccga
     4021 ggctcaagga ggagttggcc gtccgcggcg agcccgtggc cggcacgatc agggtcaagg
     4081 tgcgggggag agccgcaacc agcccacggg gtcgttgacc ccgggattgg ccctatgcgt
     4141 gagctgcgga cgcggcggcc acgtacgaaa tgggcgcgtc ctgcagcgtc tcgaacgtga
     4201 cgtcttccca cgccgcgctg tcggcgatga gcgcccgcag caggcgattg ttgaggccgt
     4261 gcccggactt gtatccgctg aactcgccga tcaggccgtg gccgagcagg tagaggtcgc
     4321 cgatcgcgtc gaggaccttg tgcttcacga actcgtcctc gtagcgcagg ccgtcctcgt
     4381 tcaagatccg gtagtcgtcg agcacgatcg cgttgtcgag gttgccgccg agggcgaggt
     4441 tgcgcgatcg catgtactcg aggtcgcgca tgaagccgaa ggtgcgggcc cggctcactt
     4501 cgcgcaggaa ggacgtcgtc gagaactcca tcgacgccac ctgcgaccgg cgcttgaaga
     4561 cgggatggtc gaactcgatc tcgaagttga ccctgaaacc gtcgtgcggg tcgaagcgcg
     4621 cccatttctc gccgtcctcg accatgacgc tcctgcggat gcgcacgaac ttcttcggcg
     4681 cattctgttc ctcgatgccg gccgactgca gcaggaacac gaacggtccc gcgctgccgt
     4741 ccatgatcgg gacttcgtcc gacgacaact cgatgatcgc gttgtcgatg ccgagccccg
     4801 ccagcgccga cagcaggtgc tcgatcgtcg agaccttcac gccgtccttc acgatcgtgg
     4861 tgccgagcgt cgtgtccccc accgcctcgg cgcgcgccgg gatgtccacc ggctcgtcga
     4921 ggtccgtgcg gcggaaccag atgccgtggt tggccggggc gggcctgagc accatcagca
     4981 ccttcttgcc ggtgtgcagg ccgacgccgg tggcgcggat cgagttcttg agggttcgtt
     5041 gcttcagcat gccagggtcc tggtcgcgct cgcggagcgc ccccctgaaa agttagcgaa
     5101 aaaggatatc acagccgtcc ttgatcacgt aactcgttga tttgacgaga ttgtctgcgt
     5161 caccgaggcc cgggcggcgg cgcctcgaga cccgggaagg ggcttcccgg gcctcgatac
     5221 gccttgggcc acgacctgcg ctggtcacgc ggctcagtcg gcctgccggc gcaggaaggc
     5281 cgggatgtcc aggaacgact cggcgttcgg gtcggcacgc aggtcatcgc cgaccgcgcg
     5341 ctgccggcgg cgcaggttgg ccggggtctc gtagtccgcg taggtctcga gcgggcggcg
     5401 cgcggcgcga ccaccggcca cgacctcgac cttgggctgc gcgccctgcg gcagcccgcg
     5461 gatcgctgcg ggccggccga tgccggtcgc gacgaccgtg acccggatct ggtcggtcat
     5521 ctccggatcg atcacgtagc cgacgacgac cgtggcatct tccgaggcga actgcttgat
     5581 cgtgtcgccg accacgtgga actcgctggt gcgcaggtcc atgccggccg tcacgttgat
     5641 cagcaggccg tgcgcgccgg agaggttcac gtcctcgagc agcgggctcg agatcgccgc
     5701 ttcggccgcc gcgcgcgcgc gattctcgcc cgtggccgcg cccgatccca tcatcgccat
     5761 gccggtctcg gccatcaccg tgcgcacgtc ggcgaagtcc acgttgatga ggcccggccg
     5821 ggtgatcagc tcggcgatgc cctgcaccgc gccctgcagc acctggttcg cggacttgaa
     5881 ggcgtcgagc agcgtcgtgt cgccgccgag caccgtgagc agcttctggt tcgggatggt
     5941 gatcagcgag tccacgtact tgcccagttc gccgatgccc tggtcggcga gcgcgaggcg
     6001 cttgttgcct tccatctcga agggcttggt gacgaccgcg acggtgagga tgccgagttc
     6061 cttcgccacc tgcgccacga ccggcgccgc accggtgccc gtcccgccgc ccatgccggc
     6121 ggtgatgaag atcatgtcgg agccttcgac cagctcgacg atgcggtcgc gatcctccat
     6181 cgcggcctgg cggccgacct ccgggtcggc gcccgcgccg agtcccttgg tgatgttctg
     6241 gccgatctgc aggctcatct tcaccttcga gtgcttcagt gcctgcgagt cggtgttgat
     6301 gcagacgaag tccacgccct cgatgcccga gctgaccatg tgagtgaccg cattgccacc
     6361 gccgccgccg actccgacga ccttgatgac ggccccctga ttttcagtgt ctgcaagtac
     6421 gaacattgtt tgctgctcca gtgatgagtg tgaccagtga ctcgagatcc gtgactgacc
     6481 cgtgatccgc gatcatttgg gcgcggccat gccgccgccc gaattcctta ccgccccttc
     6541 cgccgccttc ctcacgacct gccctcctcg ttccgcccgc ctccgctacc cgcctgcctc
     6601 gatccgccgt cggccgtccg ccgaccgctg ccctagaaat tcccctggaa ccacgcgcgc
     6661 atccggtcca cgacgctgcg catgccgttc gcgacgggcg cggtgcggcc gcggcgcacg
     6721 tagttctcgc gcccgaacag cagcaatccg acgccggtgg cgtggatggg attgcgcacg
     6781 acgtccacga gcccgctcac gccctgcggc agcccgagcc gcaccggcgc gtggaagacc
     6841 tcctccgcga ggtcgatgac gccttccatc ttggcgctgc cgccggtgag cacgacgccg
     6901 gccgcgatca tctcgtcgaa gcccgagcgg cgcagttcct cgcgcaccag ctggaacagc
     6961 tcctcgtagc gcggctcgac gacctcggcg agcgtctggc gcgcgaggcg gcgcgccggc
     7021 cggtcgccca cgctcggcac ctcgatcgtc tcgtccgggt tggcgagctg cgacagcgcg
     7081 cacgcgtagc ggaccttgat ctcctcggcg tggtgcgtcg gggtgcgcag gctcacggcg
     7141 atgtcgttgg tgacctggtc gccggcgatg ggaatcaccg cggtgtggcg gatcgccccg
     7201 cccgagaaca cggcgatgtc cgtggtgccg ccgccgacgt ccacgaggca cacgccgagc
     7261 tccttctcgt cgtcggccag caccgcatag ctcgacgcga gctgctcgag cacgatgtcc
     7321 tcgacctcga gcccgcagcg ctgcacgcac ttgacgatgt tctgcgccgc gctgtcggcg
     7381 ccggtgacga tgtgcacctt cgcctcgagg cgcacgccgg acatgccgat cggctcgcgg
     7441 atgccctcct gcgagtcgat gatgaattcc tgcggcagga tgtgcaggat cttctggtcg
     7501 gccgggatcg ccaccgcgcg cgccgcgtcg atcacgcggt ccacgtcccc gggcatcact
     7561 tccttgtcgc ggatcgcgac gatgccgtgc gagttgagcg agcgcacgtg cgaacccgcg
     7621 atgccggcga acaccgagtg gatctcgcag ccggccatca attccgcctc ttccaccgcg
     7681 cgctggatgg actgcacggt cgagtcgatg ttcacgacca cgcccttctt gaggccgcgc
     7741 gaggggtgcg tgcccaggcc caccacctcg atcgtcccgt cgaggccgag ctcgccgacg
     7801 atcgccacca ccttggacgt gccgatgtcg agcccgacga tcatgttgcg ttcagtcttc
     7861 ttaggcatcg ggtgtcgcgg cctcctgcgg tgccacggcg agggcggaac gcgcgttcca
     7921 gccgacgctg aatccattgg tgtagcgcat gtcgacgtac ttgatctccg cgagccgctt
     7981 ggagaccagc gggctcgcga gccggatgaa ttgcccgagc cgctcgttca cggcctgccg
     8041 gccgagcttg accacgacgc cgttggtgag ctgcagctcc cacgcgccgc gttcgtcgag
     8101 gcgcacgccg gtgaggcgca gcccggcttc gagcagcggg ccctgcgcct cgaggtacaa
     8161 ctgcgcgacg cggtgttcgc tgccgtccgg gccctgcagc agcggcagct cgggcggcac
     8221 gtagcgcgcg ttgcggacga acagttcccc gcgtgcgttc agcagccccg cctcgttcca
     8281 gcgcgccgcg gccacctgct cggtgacggt cacccggatc gcgtccggcc agcggcgctg
     8341 gaccacggcg tggtcgaccc actcgatgcg ctcgatgcgc tcgcgcacca ggtcgaggtc
     8401 caccgacgcg aggccaccgt gcacgacctc gacgaccgcg ctctcgatct ccgggggcga
     8461 gacgcgctgg aacgcgccct cgaccagcac gcggcgcacc gggcggtcga gcgccagcac
     8521 cagcagcagc gcgaccagcg ccaggcccgc gaggatgccg gcggcggcgc cgagcgcgcg
     8581 ccagttgacc cgcgcaacca gggactgctc cccgttgcgg ggctggcggc ggttgcgctt
     8641 tttccagaat gccagcatcc gtgatcctca gtccctgcgc gcgacgcagc tcgtctcgag
     8701 cacgcgccac accagctcgt cgaactcgat gccgcgcatg cgggccgcct tgggcacgag
     8761 cgagtggctc gtcatgccgg gcgcggtatt gacctcgagc agccacggct tgcccgcgcg
     8821 gtcgcgcatg acgtcgacgc ggccccatcc gctgcagccg acggcgaaga acgcggcgag
     8881 cgcgaggctg ccgaactcct gctcgacggc gccggtcgcg cccgggcaga cgtactgggt
     8941 gtcctcggcg acgtacttgg cgtggtagtc gtagtactcg ccggccggca cgatgcggat
     9001 cgaaggcagc gcatccgtgc ccagcacggc gacggtgtat tcgtctccca cgatcagctg
     9061 ctcgacgagc atcggcccgg cgtagcgcgc agccagctcc accgccgccg cgaggtccgc
     9121 ttccttgaag cagcgcgaga tgccgacgct cgatccctcc agggcgggct tgacgatgac
     9181 cggcagcccg agcttgtgcg ccgccgccac gacgtcgtcg ccgcgctcga ggctcacgta
     9241 gcccggcgtc ggcaggccga gcgccatcca cacctgcttg gtgcggatct tgtccatcga
     9301 cagcgccgag ccgagcacgc cggaaccggt gtacggcacg ccgaacgcat cgagcaatcc
     9361 ctgcaggatg ccgtcctcgc cgccgccacg atgcccgtgc aggatgttga atacacggtc
     9421 gatccggcga ccagcggccg gcgcgaccag ctccgcaacg agccccggga ttccgtccac
     9481 ggcaaacgcg tcgacgccgc gccgcaccag cgcctcgagc acgttgcgcc ccgaatcgag
     9541 cgagacctcg cgctccgaag aggtcccgcc catcaccacg gcgacgcggc cgaagtcccg
     9601 cggatcggtg atgcgatgcg atgcgtgcgt catgcgcgcc ctccctgggt cgcgatttct
     9661 gccagggcgg caggaagctc ctgggcagcg gcgccgatgc tgccggcgcc catcgtcagc
     9721 acgacatcgc cgtcgcgcgc gacgccggcg agtgcctgcg gcagctcgtc gagcgaatgc
     9781 agcaggaccg gctcgacgcg cccgcggctg cggatcgcgc ggcacagcgc cttgctgtcg
     9841 gcgcccgcga tcggcgcttc accggccgga tacacctcgg ccagcagcag cgcatccgca
     9901 tcggaaagaa cccgcgcgaa ctcgtcgagc aggtcgcgcg tgcgcgtata gcggtgcggc
     9961 tggaacacca gcacgatgcg gcgatcgggc cagccctgtc gcgccgcttc catcgtcgcc
    10021 gcgatctccg tcgggtgatg cccgtagtcg tcgaccaggg tcacgcgccc ggcgggcgcc
    10081 tgcacgtcgc cgagccactg caggcggcgg tcgatgccct ggaaaccggc gagcgcgcgc
    10141 tggatcgcgg cgtcgtcgat ctcgagctcc gtcgccacgc agatcgcggc gagcgcattg
    10201 agcacgttgt gccgtcccgg caggttgagc gagacgttga gcagtggccg tccagggcga
    10261 cgcacgtcga acaccgactg cgcaccgcgg cgcgtgacgt tcatcgcacg cacgtccgcg
    10321 gactcgtcga acccgtaggt gacgtacggc cgggcgatct gcgggatcag gctcgcggcc
    10381 tccgcgtcgt cgctgcagac gatcgccagc ccgtagaacg gcaggttgtg caggaactcg
    10441 atgaagctgg ccttgagccg gccgaagtcg ccgccgtgcg tgccgaggtg atcggcgtcc
    10501 acgttggtga cgatcgcaat caccggctgc aggtgcagga acgaggcgtc gctctcgtcg
    10561 gcctccgcga cgaggtaccg gcccgcgccg aggcgcgcat tggccgccgc gctcttcagc
    10621 ctgccgccga tgacgaacgt cgggtcctcg ccgccctcgg cgagcacgct cgcgacgagg
    10681 ctcgtggtcg tggtcttgcc gtgcgtcccc gccacggcga tcgagtgccg gaagcgcatc
    10741 agctcgccga gcatctcggc gcgcttgacg accgggacgc gctgcgcgag cgcctgcgcg
    10801 acttccgggt tctccgcgtt gatcgcgctc gacacgacga cgacgtcggc ccccgcgacg
    10861 ttctcggccc ggtgaccgat catcacccgc gcgccgagct tctcgagccg gcgcgtcgcc
    10921 tcgccctgct tcagatccga cccttgcact gtgtatccga ggttgaccag cacctccgcg
    10981 atgccgctca tgccgctgcc gccgatcccg acgaagtgga tggtctgtat gcggcgcatc
    11041 cggtccctca tgccgcacct cccgccaccg cgacgcaggc gtcggccagc cgttcgtccg
    11101 catccgtgat cgccgacgcg cgcgcggcgc aggccatgcg ctcgagccgg ggccgcccgg
    11161 cctcgagcag ctcgcgcagc ttcgcggcga gcgacagcgg ggtgagtcct gcctcggcca
    11221 ccagcaccgc cgcaccggcg cggaccatgt gctgggcatt gcgagtctgg tgatcgtcca
    11281 cggcggacgg gaacggcacg agcaccgcag gcacgccggc ggccgtgagc tcggcgatcg
    11341 tgagcgcgcc cgcgcggcac acggccaggt cggcccagcc gtaggcgctc gccatgtcgt
    11401 cgatgaacgc gcgcacgtcg gcctcgatcc cgcgcgcgcc ataagccgcc gcggtatccg
    11461 ccgcgccccg aaccccggtt tggtgcagga cgtccggccg cagccagtcg ggcagctccg
    11521 cgatcgcggc gggaaccacg gcgttgagtc gcgcggcgcc ctggctgccg ccgaagacga
    11581 gcaatcgcgc ccggccttcg cggcccgcgt aacgacgctc cggcggcgcg atgtcggcga
    11641 tctccttgcg caccgggttg ccgacgcact ccgccttcac gcccttgccg aaactgccag
    11701 ggaatccctc gagcacccgg tcggccagcc gcgcgagcag gcgattggtg aggccggcga
    11761 tggcgttctg ctcgtgcacg acgagcggcg tgcgcgtgag ccaggccgcg acgccgcccg
    11821 gtcccgacac gaatccgcct gcgccgagga cgacgcgcgg cttgtgcctg cgcaccgcac
    11881 gcaatgcctg catcaacgcg gtggcaagct tgaacggagc cgcgaggagt gtcgtgatgc
    11941 ccttgccgcg caggccgccg acgctcagcc actcgacggg gatgccggcg gcgggcacga
    12001 gccgcgcctc gatgccgcgc tgggtgccga gccacacgat gtcgtagccg cggcgctgca
    12061 ggacgcgcgc cgtcgcgagc gccgggaaca cgtggccgcc cgtgccgccc gccatgatca
    12121 ttgcggtggg acggctcatt cgcggcgcgc ctcccgcgac gcggccggcc gcccggcctg
    12181 ctgcgtctcg tggttgatgc gcagcagcag cccgatgacg cccagcgtga ccagcatgct
    12241 gctgccgccg taggacagca gcggcagggt gagccccttg gtcggcagca ggcccatgtt
    12301 gacgccgatg ttcacgaacg cctgcagtcc gagccagatg ccgatcgacg cggcgaggca
    12361 ggactggaac ggcaggtcgg catcggcggc gcggcgcgag atctcgagcg cgcggccgac
    12421 cagcagcgca aagagcccga tcacgacgca cacgcccagg aacccgaact cctccgccag
    12481 cacggcgaac acgaagtcgg tgtgcgcctc gggcaggtag aacagcttct gcacgctcga
    12541 gcccaggccg acgccgaacc actcgccgcg cccgatggcg atgagcgact gcacgagctg
    12601 gaagccgctg tcgaacgggt cttcccaggg attgcggaat gccgtgaggc gacgcatgcg
    12661 gtacgacgag gtcacggcca gcagcgccat gccggcgacg ccgaccacca ccggcacgag
    12721 caagtggtgc agccgggccc cggccaggaa cagcaccgcg agacccgtgg ccatgagcac
    12781 ggtcgcggcg ccgaagtccg gttcgagcag cagcagcacc gcggcggcgc cgagcacgac
    12841 caccggcttg atgaagccct tgaagtccga gcacagttcc tcggagcggc gcacggcgta
    12901 gctcgcgacg taggtgagga gcatcacccg cgcgagctcg gagacctgga agttcatgaa
    12961 gcccagccgc gcccagcgcc ggctgccgtt gacctcgtgg ccgatgcccg gcaccagcac
    13021 cagcagcagc agcgcgaagg cgccgatcag cagcacgatc gagtgcttct cccagatccg
    13081 cgtcgggatg gccatcgcga ccagcgctgc caccaggccc atgccgacgt agaacaactg
    13141 gtgctcgaag taatagaacg ggttgccgga ctcgcgggcg gacacgccga tcgacgccga
    13201 ggcgaccatg accaggccca ccagcagcag ggcggccacc gtgccgatga cccaggcgtc
    13261 cagggcgtag cgccgcggct ggccggtgga gcgcgcgtag gagatggtcg tcgacgtcat
    13321 gcggcgaggc tcctcaccgc ggtggcgaac tcgtcgccgc ggtgcgcgta gtcacggaac
    13381 atgtcgaggc tggcgcaggc cggcgagagc agcacggtgt cgccggggcg ggcgaccgcc
    13441 cgcgcagccg ccaccgcgga cgccatgtcg gctgcgctct cggtcgtgca gacgccctcg
    13501 agcacggcgg cgagtcgcgg accgtcgcgc ccgatcagca cggcgtgccg gaccttgccg
    13561 gcaaatgcgg cgcgcaggtc gccgaagtcc tggcccttgc cgtccccgcc cgcgatcagc
    13621 acgaccggac cgctcgtgcc ggtgaccgcg gcgatcgtcg ccccgacgtt ggtccccttc
    13681 gaatcgttca cgaagtgcac gccggccacg tcgcgcaccc actgcgcgcg gtgcgcgagc
    13741 ccgccgaacc cggcgagcgc ctcgagcacc ggggcgcgcg gcagtccgag ggcttcgcac
    13801 atggcgagcg cggcgagcgc gttggccacg ttgtgcaggc ccttgatgcg cagcgcggac
    13861 accggcagca acggctcgcc gcggcacgcg agagtcggtt gcgcaccgtc cgcgagcgtg
    13921 tagtccgcgg cctcgtccat gagcccgaac gacagcaccc gctggccggg ccgcggcatc
    13981 tcgcgcacga gcgggtcgtc ggcgttgacc acggcgacct cgcagccgtc gaagatccgc
    14041 gccttcgcct ctgcgtagtc cgcgagcgtg tcgtagcggt ccatgtggtc gggagtgacg
    14101 ttcagcaccg tggcggtcgc cgtggcgagc gagtacgtcg tctcgagctg gaagctcgac
    14161 agctcgagca cgtacagcgc cggcacgggc cgctcgagca ggtcgagcgc cggttcgccg
    14221 agattggcgc cggccacggc cggttgcccg gcagcggtcg cgagttcggc gagcagcgcg
    14281 gtgacggtgc tcttgccgtt cgtcccggtg atcgcggcga cgggcgcctg ggcttcgcgg
    14341 gcaaacagct cgatgtcgcc gaccagcggc aggccgcgct gctgcgcctc gcgtgccacc
    14401 ggttccttga ccgagagccc gggggaaatc acgacctgcg cgacgtcgtc gagcagcccg
    14461 gacgcgaacg cccccgtgcg cacctcggcc tccggcacgt cgctcagcag cttcgcgacc
    14521 cccggcggct cggcgcgcgt gtccgcgacg cgcacgcgca ggcccttgcg cttcaggaaa
    14581 cgcgccaccg acaacccggt gcggccgagc ccgagcacca gcgcgcggga gcccggctcg
    14641 agcggcgcga gacttgtcgc gtgagccgtc atcgcagctt cagggtcgcc agcccgatca
    14701 gcacgaggat cacggtgatg atccagaagc gcacgatcac cttcggctcg gcccagccct
    14761 tgagttcgta gtggtggtgg atgggcgcca tgcggaagat gcgcttgccg cgcagcttga
    14821 aggagcccac ctgcaggatg acggagacgg tctccatcac gaacacgccg cccatgatga
    14881 agagcacgat ctcctgccgg acgatgacgg cgatgacgcc gagcgcggca ccgagcgcga
    14941 gcgccccgat gtctcccatg aacacctggg ccgggtaggt gttgaaccac aggaagccga
    15001 ggcccgcgcc gaagatcgcc gcgcagatca ccagcacctc gccggcaccg gcgatgtaag
    15061 ggatgccgag gtagttggcg aacaccacgt tgcccgacgc gtaggcgaac acgccgagtg
    15121 ctccggcgac catcaccgcc ggcatgatcg ccagcccgtc gaggccgtcg gtcaggttca
    15181 cggcgttgct cgtgccgacg atcacgaagt acgcgagcac gatgaacagc gggccgagcg
    15241 gcagcacgaa gctcttcacg aacggcacga tcagcgtcgt ctcggccgcc gtgcgcgccg
    15301 tcaggtacag cgcgatcgcc gcaccgagcc cgaccacggt ctgccagagg tacttgtagc
    15361 gcgcgatcag cccgcgcgaa ttgccgacca cgagcttcag gtagtcgtcc cagaagccga
    15421 tcaggccgaa cgccaccgtc acgccgagca cgatccacac caggcggttc gcgaggtcgg
    15481 cccacaacag ggttcccacg acgatcgcga cgaggatcag cgcgcctccc atcgtcggcg
    15541 ttcccgcctt cgacaggtgg ctctgcggac cgtccgagcg cacgcgctgg ccgacctggc
    15601 gctctgcgag ccggcggatc atgcccggcc cgaccatgaa ggaaatggtg agtgccgtga
    15661 gcgccgcgag gatcgcgcgg aacgtcaggt agttgaagac gcggaagccg gagtagtact
    15721 gggcgagcca ctcggcgacg tgacgcagca tgctagtggc ctccccggcg cggcgcgccc
    15781 tcggcggcca gtgcatcggc gacgcgctcg aggcgattcg agcgtgatcc cttgaccagc
    15841 accgtcacgc cggaccgcag gtgcggccgc gcggcggcga ccagttcctc gacgtcggcg
    15901 aaccacaggc cgcccgcgcc gtacgcctcg accgcgcggc gcgcatcctc gccgaccgcg
    15961 aacaggcggc gtatgccgct cgtgcgggcg agatccccga tctcctcgtg caggcgcgcc
    16021 gagtcctcgc cgagctcgcg catctcgccg agcaccagcc agtgctcgcc gggcacggtt
    16081 gcgagcgcct tcaggcccgc gcgcacggag cccgggttcg cgttgtagga gtcgtcgacg
    16141 aggcgggcac cgttgagcgc ggcgcgcagt tgcagccggc ccgcgaccgg acgcacggac
    16201 ccgaggccgg cggtgatcgc gtcgaggctg gcgccggccg ccgccgcggt cgccgcggcc
    16261 gcgagcgcgt tcatgacgtt gtgctccccg gcgagcgcga tggatacgcg ggaccgcccg
    16321 agcgggcacg tgaggtcgaa gtccgtgacg aacccgtcgt cggccaggcg acactccacg
    16381 ccgccggcca tgaagtcagc gtgctcccgg acgccgaagg tgacgatccg ccccgcttcg
    16441 cgcgcgagtc cgtgccagaa cgcggcgtac cggtcgtccg cgttgaccac cgccgcacat
    16501 tccacgccga gcgcctcgaa catctcgccc ttgccgcgcg cgacgccttc gatgccacca
    16561 aagccctcga ggtgggccgg acccgcgttg atcacgaggc cgacgtcggg ccgcgcgatg
    16621 gacgcgaggt gcgcgatctc gccgcgatgg ttggcaccca tctcgatcac cgcgtagcgg
    16681 tgccccgcct ccaggcgcgc gagcgtcagc gggacgccga tgtggttgtt caggttgccc
    16741 tgcgtcacca ggcaggggcc gcagcggccg agaatcgcgc cgatcatttc cttgaccgtg
    16801 gtcttgccgt tgctgccggt gagcccgacc accgtgccgg agaagtcgtg tcgccatgcg
    16861 cgcgcgaaag ccgtcagggc ctcgagcgtg tccgcgacca cgacctgcgg gacggccgcc
    16921 ttgaccttgc ggctgacgac ggcaccggcg cagccgcgct ccgcggcctg ccggacgaag
    16981 tcgtgcgcat cgaatcgctc gccacgcagc gcgacgaaca gctcgccgcg ctccacggcg
    17041 cgactgtcgg tggtgacggc cccgaacgcg gcattggcgc cgtcgagcct gccgccggcg
    17101 acgcgcgcga attcggcgag gtcccggatc acgcctcgct ccccagtgcg tcgagcacga
    17161 ccttgcgatc gctgaacggg cgcacgtcgc ggccgacgac ctggtagtcc tcgtggccct
    17221 tgccggcgat caccacgacg tcgccggcgt cggcctccgc cagcgcgtgg tggatggcct
    17281 ccgcacggtc ggcgatcacg tgcgcggcgt ccggctcgcg catgccgccg aggatctgct
    17341 cgatgatcga gcgcgaatcc tcggtgcgcg ggttgtcgtc ggtgacgacg acgacgtcgg
    17401 cgcgctgctc ggcgatcgat cccatgatcg gccgcttgcc cggatcgcga tcaccgccgc
    17461 agccgaacac gcagtacagc cggcctcgcg cgtgggcgcg cgcggcatcg agcaccttgg
    17521 cgagcgcatc cggcgagtgg gcgtagtcga cgagcgcgag tggctgtgcg ccgccgccga
    17581 acgcctccat gcggcccggg ggcggggcgc acgtcgcaag tgcggcgagc gcctgctgca
    17641 gcgggacttt ccacccgagc agcacgccga ggacggccag caggttgtcc gcattgaact
    17701 cgcccaccag gcgcgagcgc agcgtcccgg cgccccagct cgactcgacg tgcagcgtga
    17761 gtcccgccgt cgtcgcacgc agttcgggga gccggatcca cgcgctgccg gccggcgccc
    17821 agacctcgtt cgagctcgaa tagaagacgc gctcgatgga cgcatcgagc cggtcggcga
    17881 gcgcgcgccc gaactcgtcg cgcacgttga cgactgcgcc gcgcagcccc ggggttgcga
    17941 acaggcgcgc cttggcggcg ccgtaggcct cgagcgagcc gtgatagtcc aggtggtcgc
    18001 gcgtcaggtt ggtgaagacc gccgtgtcga agcgcacgcc atcgacccgc ccctggtcga
    18061 gcgcatgcga cgacacctcg atgcccatcg tcttcgcgcc cgcatcgcgc gcctgcgcca
    18121 ggcgccggtg cacgttcacg caatccgggg tcgtgaggcc ggcatccacg agcgcgcccg
    18181 ggcggccgta accgatggtg cccaggtacg cgccgcggcg gccgaccgca tcggcggcct
    18241 gcgccagcag gtaggccgtc gtggtcttgc cattggtgcc ggtgatgccc gcgacggcca
    18301 gatcggcgga cggcgcccgg aagaaccggt ccgcgatcgt gccgacgcgc tgcgcgaggt
    18361 ccggtacggc ctggacgacc acgtgcccgg gcagtgccgg aacgtccacg cccgccgcag
    18421 gctcccagag caccgccact gcgccgcgcg ccacggcatc cgcggcgtgc gcaagtccat
    18481 gggtgctgcg accgcggcac gcgaggaacg cgctgccctg ggtcgcgccg cggctgtcct
    18541 gcgtcaggcc ggccacggtc aggtcgcgcg gcaggcgcac gtcatcgccg agcagcgtcg
    18601 ccagcgacgc gcgatagacc ggaggcagct cggattcaac cacggcgttc attccgctcg
    18661 attgcccatg gttcaccacg gatccccggc ccggacgagc gtcgtcgcag ggatgccctg
    18721 caggtcgtcg ggcgggatcg cgagcacgcg cagcgcgccc gacatgaccg ccgcgaacac
    18781 gggagccgcg acctcgccgc cgtagtaagc cccggcgctc ggctcgtcga tgatcaccac
    18841 cgccgcgagc cgtggattgc tcgccggcac gacgccgccg aagatggcgc ggtacttgtc
    18901 ggtcgagtag ccgcccgtct ccgacgcctt ccacgcggtg ccggtcttgc cggaaacctg
    18961 gtaacccatg atggccgccc ggcgggcggt tccggcgtcg gtcaccacgc tctgcagcat
    19021 gcgcaccagt tcgcgtgcga cgggttccgg catgacgcgt tcacccgccg gcggcgtatc
    19081 cacgcgacgc aggctgaccg gacgacggat cccgcctgcg ccgagcaccg cgtaggcctg
    19141 cgcgagctgc agcggcgtga ccgacagccc gtagccgtac gccatcgtcg cctgggagat
    19201 cgcgcgccag tgctcgtagc ccgggaggat cccggccgac tcgccgggga agcccgatcc
    19261 cgtcacgcgg ccgaagccga acgcgtcgta gacgccccac atctcctgcg gcttcagggt
    19321 gagcgcgatc ttcgccgccc cgacgttgct cgacttggcg agcaccgtgg tcacgtcgat
    19381 cgtgccgagg ttgttgtggt ccttcacggt cttgatgccg acccgcatga tcccgggcga
    19441 ggtgtcgatc agcgtgtccg cgtggaagcg cccggtctcc atgccggccg cgacgacgaa
    19501 cggcttgatg ctcgagcccg gctcgaggaa gtcgttggtc gcgcggttgc ggtaccgcga
    19561 cggcaggtac tgctcgcggt cgttcggatt gaacgccggc tggttgacca tggccaggac
    19621 ctcgccggtg gcgatgtcga gcacgacgat cgagccggcg atcgcgcggt tcgcctgcac
    19681 ggccgccttg agctcgcggt aggcgaggta ctgcacgcgc aggtcgatgc tggtgcgcag
    19741 gtcctggccc gggcgcggcg cgctcagttg ctcgatgtcc tcgatcgtcc ggccgaggct
    19801 gtcgcgcatg acgatctttt cgcccggcac gccgccgagc cactggtcgt aggcgagctc
    19861 gaggccttcc tggccgacgt cgtcgacgtt ggtgaacccg agcaggtgac ccgtcacttc
    19921 ccccgccggg tagtagcgcc ggtactcgcg cagcgtgtcg acgcccggga tgccgagtgc
    19981 cctgaccttc gccgcatcgg ccggacgcat gtgccgcacc aggtagatga attcccggtc
    20041 gaggctgctg gtcacgcggc gcgcgagcca ctgcgggtcg cgatcgagcg ccttggcgag
    20101 gcgcgggaat tcctgcggga cctcggcgag cttgcgcggg tcggcccaca cggtgtcgac
    20161 gggcgtgctg accgcgagtg cctcgccgtt gcggtccagg atcatcccgc ggttcgccga
    20221 cagcgtggcc ttgcgcagga tgcgcgcgtc gccctgctgc tcgaggaacg cctggtcgac
    20281 cacctgcagg tacacggagc gcgcgagcag cgtgaggccg gcggcggcca gcgcgcccag
    20341 caccagccag tggcgccagg gctggcggcc ggggctgcgg tcccgtttcg agtcgcggat
    20401 catggctgga cgatgcgcag gtcggtggcc tgcgggatga ccatgtggag gtcgccgcgg
    20461 gcgacgctct cgacgcggcc gttggtcgcc cacgtgctct gctcgagctg cagctgtccc
    20521 cactcgatgt cgaggtcgtc gcgctccttg gtgaggcgct gcagctcgta gaagcggctg
    20581 cgcgcctcgt gcttggcgaa gatgacgccg agcgccgagg cgagcagcgc cagcccgagc
    20641 agcgacaccg cgcagcgcca ggcgacggtg ctcatgcggc aagcctctcg gccacgcgca
    20701 gcacggcgct gcgcgcccgc ggattgcgtg cgacttccgc ctcgcccgcc tcgatcgcct
    20761 tgccgacgcg cttcaggcgc ggtcgcgcat gcggcggcac ctgcgcgtcc ggcaggcctg
    20821 cgtacacggg atcaccctgc tcctcgcgcc ggatgtagcg cttgacgatg cgatcctcga
    20881 gggagtgaaa gctgatcacg cacagccgtc cgcccggcgc gagcagccgc accgcctgcg
    20941 gcagcgcggc ctcgagcgcc tcgagctcac cgttcaagtg gatgcggatc gcctggaacg
    21001 tgcgcgtggc gggatgcttg ccgggctcgc gggtgggaac ggcggcggcc acgatcccgg
    21061 cgagctggcc ggtgcgctcg atcgggcgct cggcgcgtgc cgcgacgatc gcgcgcgcga
    21121 tgcggttcgc gtagcggtcc tcgccgagcg agcgcacgac cgcggcgatc tcccgttcgt
    21181 ccgcgcgcgc gatccactgc gccgcgctct gccccgcctc gttgtccatg cgcatgtcga
    21241 gcggcccgtc ctgcatgaag ctgaagccgc gcttcgcttc gtcgagctgc ggcgaggaga
    21301 caccgaggtc catgagcacg ccgttcaact ccccttccag tcccgtcgca cggaccagct
    21361 cctcgagccg gccgaacgac cccttcacca gcgcgagacg tggctccccc tggaagcgcg
    21421 ctgcaccgga cctgatcgcc tccgggtccc ggtcgatggc gatcacgcgg ccttgcggcc
    21481 ccagcgcgcg caggatcgcg gccgtgtgcc cgccccgccc gaacgtcgcg tcgcagtacc
    21541 gaccgtcggc ccgcaccgcg agcccggcca gcacttcgtc gggaagtacc ggcgtgtgct
    21601 ccgccacgac cgcgcctaga gcgagagctg ctcgagctca ggaggcagtt cgcccgtctc
    21661 ctcgcccgcc tgcagccacg cctcgcggcg cgcgttccag tgttgctcgt cccaaagctc
    21721 gaaacggttg ccctggccta tcagcgtggc ctggcgcgtg agtccggcgt actcgcgcag
    21781 cttcggcggc accagcaccc ggccgtggga gtcgagctcc aggtcggtcg cgtggccgac
    21841 catcagcctc tgcagccgcc gcgcctgggg attcaggctc gggagccgca tgagcttgcg
    21901 ctcgatctct tcccagtccg ggagcgggta gatcagcagg caccggtcgg cccggtcgac
    21961 ggtcgcgacc agacggccct cgctgcgctc gaccagggtg tcccggtatc gcgcaggcat
    22021 ggccatccgc cctttggcgt cgagagtgac tttgttggcg ccgcgaaaca tttcaggtcg
    22081 ctgatgggcc catcgcccat ttcatcccac tttgtcccac cgcggcggaa tataggtcgc
    22141 gcgcgcgaag agagtcaacg gcccaggcaa aagattttcc atgaggcaca gcgacttaga
    22201 cccagtcagc tcacggtcgc tcgaggcctc taggaactat cgttatttta atcaaatagt
    22261 taccggagtt ttctgaaaac aacttgagat atcagctcct gtgggaggaa accgctggac
    22321 caagggatat gttggggcgc ccggtgccgg gctggcgacc gccggcaatt ccattgccgg
    22381 gtgttgtggg acggccggtg cgtgaccgcc gcccggttga aggctcggcg gcctgcgatc
    22441 gaggtgggac gcggtgggcg atgtcgcagg tccgctacgc ccagggcgag ccacccatgg
    22501 acagccgcac gaaactattg acagtctcgc ttaattgtga ctgccggaag tgggagtcgg
    22561 ccgataagcc gggttctgtc gtgggcaacc atccatctgg gacgcgcgtc gccgcgcgcc
    22621 tcgagcgacc tacccggaag cctccgcgag ccgcgggtgc gggcccgaaa gcccgcgcgc
    22681 ttccctattt ggtcttgctc caggtggggt ttgccgtgcc gccgaatgtt gccaccggcg
    22741 cggtgcgctc ttaccgcacc gtttcaccct tgccgtcgcc gcatcgctgc ggcgcttggg
    22801 cggtctgctc tctgttgcac tttccgtggg ctcgcgcccc ccaggcgtta cctggcacct
    22861 tgctctgtgg agcccggact ttcctccgcg gaataccgcg gcgattgcct ggccgactcc
    22921 cgcgtccagt ataccgcggc gaccccgcga atccgttcat tcctcgcgtt gcgcgatggc
    22981 gagcgcccgc tcgtagagtt ccttgcgcgg tcgcccggtg atcgtcgcgg cgagccttgc
    23041 ggcctgcgac acggtcacct ccgcgagcag cgcgcgcagg atgcggtcgg aatcccgcga
    23101 atcctcgtcg ctcgattgcg ccgccccgtg cacgacgatc acgatctccc cgcgcgccat
    23161 gtccggctcg agcgcggctc gacgcgccag gtccgccagc gtcccgcgat acgcactctc
    23221 gaagcgcttg gtgatctcgc gcgcgatggc cgcggtccgc tcgccgccga ggcattgcgc
    23281 gaggtccgca agcgcctcct ccagccggtg cggcgcttcg tagaacacca gcgtgcgcgg
    23341 ctcggtcgcg agcgactcga gcgcgcggcg gcgcgccgcg gcgcgcgccg gcaggaaccc
    23401 ctcgaaggcg aatcgctcgg ccggcagtcc cgacgtcgag agcgcggcga tggctgcaca
    23461 cggacccggc acggccacga cctcgacgcc cgcgtcgatc gcggcgcgga ccagcggcgc
    23521 accggggtcg ctcaccagcg gcgtgcccgc gtcgctcacg agcgcgaccc gggctcccgc
    23581 ccggagccgc tccaggacac gcggcacccg tgagcgctcg ttgtgctcgt gcagggattc
    23641 gacgctgccg ggcggccgct cgatcccgca agcgtgcagc aggcgcgcgg tgtggcgcgt
    23701 gtcctccgcc agcagcaggt ccgcgtcgcg cagcaggtcg cgagccctcg ggctcaggtc
    23761 gccgaggttg ccgatcggcg tcgcgaccag gaacagtgcg ccgtgtcgcg gccccgctat
    23821 agaatccacc ggttccttcg ccacgcgcgc atcctccggg gccccatgct gcacgcaagc
    23881 cttcgcggcc ggcaagttcg agcgttgctg ctcaccgtcg cactggcggc cggcgctgcg
    23941 caactgccgg cgccggcccg cgccgatgcg ggcgagcaac tttcgcaggc cgccgcactg
    24001 tcgcgcgatg ggcagcacga ggcggcggcc cgaatctacg agcagcaggc gcggcgggtg
    24061 ttccgcgcgt gggacgcgcg cctgtcactg ctcgcggctc gcgagtacct cgaggcgggt
    24121 cgcacgagcg acgccgcccg cgtgctctcc aaggtgacgg gaccgctgcg cggcgatgac
    24181 atcgtgctgt acgggcgcgt caaggcggaa ctcgcgctgg ccaggggaga cggtcccgcg
    24241 gcgctcggcg cgctcgctgc cattcccagg ccgtggcccg caccgcttgc tcaagagctg
    24301 ctgctgctcg agggccgcgc cgactttctc gcgggccgga ccctggaagg catccgcgcc
    24361 ttcgaggagc gcggccaggt gctcggcggc gccgaagcgc gcgcggaaaa ctatcgcctg
    24421 ctcgtcgatg aactcgggcg cagtgatgcc gtgctcgccg ttccggccgg ggccaccgac
    24481 ggcgagcggg cctggatcga actcgcgcag atccgcgcga tgtcgcaggg cgatgcggcc
    24541 gtggtggcgc agcgggccgc cgattggcgc actcggcacc ccaaccatcc cggcgccgcc
    24601 ttccttcctc aacccgcgag caacgctggc ggagtggcgg tgccggtggc tgcgcccgga
    24661 cagggatctg cgacggtggc cctgctgctt ccgctgtcgg gacggcaaca ggccgtcggg
    24721 gtcgcggtcc gcgacggctt catcgcggcc agcctcgcgc atgacgacac cgggcggaag
    24781 gtgctcgtgt acgacacggc agccctcggg ccggcgcagg cataccagcg tgcgttggcg
    24841 gacggcgcgg gcgcggtcgt cgggccactg acgcgcgaag acgtcgcggc gctctcggga
    24901 tcgcagcccc tggcggtgcc gacgctcgca ctgaacgcgt acgcgggcgc cggtgcgcca
    24961 cccgcgttcc tgtacgagtt caccctcgac ccggagcagg aagcgcgagc tgccgcgcgg
    25021 cgcatcgctg cagacgggcg cacgcacggc atcgcgctgt ttccgcgcag cccgtggggc
    25081 gagcggatct acgcggcgtt caccgcggaa ctccagcagg ccggcgtgtc gctgacgtcg
    25141 gcgcagtact acgagccggg ctcgcgcgat ttctcggggc cgctgcgcgc ggccctcggg
    25201 cgctacggcg gtgccgccga tcgcagcgac ggccgcccgc cgccgaagcg ggaccccgtc
    25261 gccgaggcgc gcgacgggcc gcagttcgcg ttcatcgccg cgaccgcgtc gacgatgcgc
    25321 accatcgtgc cgcagctgcg cttccagatg acgtacgcat tgcccgtgta tgcgacctcc
    25381 gacgcctggg accccgtgcc ccgcgcggtc cccgacctcg acggcatggt gtacccggaa
    25441 ttcccgtggg tcctgcaggg tggcgagggc gcgcggggac tctgggaagt gctgcagcac
    25501 gagtgggccg cgaacgcccg cgggcgcgtc cgcctgtacg ctttcggcca cgacgcggcg
    25561 accgtggcgg cggccatcac gtcgggccac gccgactcgc cgatcgacgg gctcacggga
    25621 cgtctcgcgc tcggcggcga cgccagggtc catcgcgaac tcgactgggc gcagatcgtg
    25681 gatggacggc cacagccagc ggcggtcacg gtatccactg cgccaccccg gtgaccaccg
    25741 agacgacgac tatcgtcggg cggcgcggcg aggacgcggc gctcgagcac ctcgccgccg
    25801 cggggctgcg actgctcgag cgcaactacc gctgcagggg cggcgagatc gatctcgtga
    25861 tggccgacgg cacgacgctc gtgctggtcg aggtgcggct gcgctcgtcg cgcgagttcg
    25921 gcggagccgc ggcgagcgtc gaccggcgca agcagcggcg cttcacgctc gccgcccggc
    25981 acctcgtgct cacccatccc gaatttcgca gcatgccggc gcgattcgac gtcgttgcgc
    26041 tcgagcgcgg cgacggcgcc ggagaaatgc gcatcgagtg gctgcgggac gcgttcagga
    26101 tgtagtgact gacggcggtc gcggacccac cgacctcgag cgccgtcact tcacgacctt
    26161 gagcttcgcg cgcccgggcg ccggcttgct ggtcgcctcg gccggtgcac cctcgggcgt
    26221 gggcccctcc ggcggggccg gagccggacc accctcgtcg tccgagaaga tcatgccctg
    26281 gcccgtctcg cgcgcgtaga tcgcgagcac cgcctcgatg ggcacccgca cgaccatgct
    26341 cacgccgccg aatcgtccgc tgaagctcac cgcgtcgttg ccgagcctga gctgcgcggt
    26401 ggcgttccag ctcacgttga ggatgatctt gccgtcccgg acatactgcc gaggcacctc
    26461 gacgccggcg acacccgcgt ccacgacgat gtgcggagtg cattcgctgt cgcagatcca
    26521 ctcgtgcatc gcacgcagca ggtacggcct gcgcgacttc aagcccgcat ctcctgctcg
    26581 atgcggctca ggctggcgcg gaatgccggg tgggcgaaca cgcgcctcgc atagcgctcg
    26641 atcgactgcg cgtcggccgg cagcccgatt tcccagcgcg caaggcgcca gaggatcggc
    26701 gccgcggccg catcgagctg cgagaactgg tcggacagga accacggccg gagcctgaac
    26761 aggggctcgg aggcaaccac gctctcgagc agcaatcggc gacccttctg ctgctcgcgg
    26821 cggtcggcgg actcgagtgc gggcgcgagt gcgtaccaat cctgctcgat gcggtgcagg
    26881 gcgaccctcg actgcgcgcg ggcggccggg tccacgggca gcagcgccgg ggccgggaat
    26941 cgctcgtcga ggtactcgca gatgattccg gcctcgtaca cgacgaggtc gcgatccacc
    27001 agcgtcggga cggactggta gggattcagg tcgatgaggt cctcgggcgg acgggccggg
    27061 tccacctcga cgatgcgcgc ctcgagcccc ttcgccgcca cgacgatccg cacgcggtgg
    27121 ctgcgcggat cgtccccacg ggagaagagc gtcatcatgg cgaaaaaggg ggcgttctcc
    27181 tgactgggga cattctccta tttcgagtcg aaataggaga atgtccccaa tttccattcc
    27241 aggagtatgt cccctgcctt gttacttcac gtccttccag tattccttct tcagcgccca
    27301 ggcgagcagc gtgaagaaca gcaggaacgc gatgaccggg tagccgagcc gtgtgcgctg
    27361 cagctgcgcg ggctcgccga tgtagtcgag gaagttcacc gtgtcgcgca cgaagccgtc
    27421 gaactcctcg gcggtcatcg tgcccggcgt gcccggctgg aacttgtcga agtgcttctg
    27481 cacgttgccc tgcgcgtcag gatgcccgac ccagacggct tcctgcacgc cctgcagctt
    27541 ccacagcacg tgcggcatgg cggtgcccgg cagcacgatg ttgttgacgc cggtcggccg
    27601 cgtcgggtcg gcatagaacg agcgcaggaa cgtgtagagg tagtccgtgc cgcgggcccg
    27661 cgcgatcagc gagaggtccg gcggcaccgt gccgaaccag cgcttggcgt cgtccgggtt
    27721 gatgccgatg cgcatcgtgc cctgcacgct gtcgccggtg aacatcaggt tgtcgatcac
    27781 ctgctgctcg gtgagcccga ggtccgccgc gatgcggttg tagcgcacgt acttggcgga
    27841 gtggcagccg aggcagtagt tgacgaagtt gcgcgcgccg cgctgcaggg acgcctggtt
    27901 cgagacgtcg gcgttgaagc ggtcgagctg cgcctcggcg cccgctgcga acgccgcgag
    27961 cggggccagc gcggcggcca ggacgaggag cttagtgcgc atggtaggtc accctcgtgg
    28021 gcacgggcct ggtcttgtcg atgctcgtgt accacggcat cagcaggaag aacgcgaagt
    28081 acaccgcggc gaacacccgc ccggcgatcg cgtacgtcgg cgtcggcggc tgcatgccga
    28141 ggtaccccag cgcaatgaag cttatggcaa acgccgcgac gaagaacttg aagatcgggc
    28201 cgcgctggcg gatcgacttc accttgccgc ggtcgagcca cgggaggaac acgaacagca
    28261 gcaccgcgag acccatgagc gcggcgccca gcggcttgtt cggcaccgag cggaggatcg
    28321 cgtagaacgg cgtgaagtac cacacgggcg cgatgtgcgg cggcgtctgc agcgcgttcg
    28381 ccggctcgaa gttcgcgtgc tcgaggaagt agccgcccat ggtcggcgcg aagaagatca
    28441 cgaggcagaa caggatcagg aacaccccga cgcccaccat gtccttcacc gtgtagtacg
    28501 ggtggaagcg gatcccgtcg agcgggtggc cgtccgcgcc cttctggctc ttgatctcca
    28561 cgccgtccgg gttgttggag cccacctcgt gcagcgcgat caggtgcgcg atcacgagga
    28621 acaccagcgc cagcggcagc gcgaccacgt gcagcgcgaa gaatcggttc agcgtgacgt
    28681 cggagatgta gaagtcgccg cggatccact cggtgagcga gtcgccgacg tacgggatgg
    28741 cgccgaacag cgacacgatc acctgcgcgc cccagtagga catgttgccc cacggcagca
    28801 ggtagccgaa gaacgcctcg cccatcagca ggaagtagat caccatgccg aagatccaca
    28861 gcagctcgcg cggcttctgg taggagccgt acagcaccgc gcggaacatg tgcaggtaga
    28921 cgacgatgaa gaaggccgag gcgccggtcg agtgcatgta ccggatcagc cagccccagt
    28981 ccacgtcgcg catgatgtac tcgacggagc cgaacgcgtc ggccgccgcg ggcttgtagt
    29041 tcatcgtaag gaagatgccg gtcaggatct ggatgaccag caccagcatc gcgagggagc
    29101 cgaagtagta ccagaagttc aggttcttcg gcgcgtagta ctccgcggcc gactcgttcc
    29161 agagcttggt catcggaaaa cgcgcgtcga tccaggcgac caggctggcg aggcggccgg
    29221 ttcccgcggg ggcgggagtc tggttcgtgc tgcccatcat gcgttccccg attcagcgcc
    29281 gatgaggatg cgcgtgtcgc tcacgaagcg gtacggcggc accggcaggt tggtcggggc
    29341 cggcacgtcc ttgaagacgc ggcccgacat gtcgaacttg gagccgtggc acgggcagaa
    29401 gaagccgccc ggccagtcgg cggaaaccgt ggggtcgccc gcctggaact tgtcgagcgg
    29461 cgcgcagccg aggtgcgtgc agacgccgac cagcaccagg tactcgggct tcagcgagcg
    29521 gttggcgttc tgcgcgaact tcggctgctc ggactccttg gagtccgggt cgcgcagccg
    29581 cgcgtcgtgc cccttgatct ccgcgagcag ctgcggcgag cggtgcacga cgtagatcgc
    29641 cttgccgcgc cactcgacct tgaccatggc gcccggctcg agcttgccca cgtcgacctc
    29701 gacgggcgcg cccatggcct tggcccgcgc gctcggcttc cacgacgcca ggaagggtac
    29761 ggccgcgaac gcggcgccga cggcgccggt tgcgatggtg gcgacggtca ggaagtgacg
    29821 cctgcccgta tcgacttctt cggtcatcca gcgctctcct cggcggattc gggtgcgtgc
    29881 cggggcggac cgcgggtgac cgccatgggc agcaaggaat tatagcggat cgcgggggcc
    29941 ggtcagcggc cgcccgaccg ggacgctgga atgggtcttg ccagggtgcg gcgggtcgct
    30001 cccccgggtc aagggtcact tcctggcccg gccgatgcgc cggatctgcg cccccagccc
    30061 gtgcagcttc tcctcgatgc actcgtagcc gcggtcgatg tggtaaatgc gctcgacctc
    30121 cgtcgtgccg tctgctacga gccccgccag cacgaggctc gccgacgccc tgaggtcggt
    30181 cgccatcacc ggggccgcgg tcagccgcgg cacgccgcgg atgatcgcgg tgttgccttc
    30241 gaggcggatc tcggcgccca tgcggcgcat ctccagcatg tgcatgaagc gattctcgaa
    30301 aatcgtctcg acgatggtcc cgacgccttc ggccaccgtg ttgagcgccg cgaattgcgc
    30361 ctgcatgtcg gtcgggaacc cggggtgcgg cgccgtgcgg atgtccaccg cgcgcggcct
    30421 cctcccccgc atgtcgacct ccacgaagcc ttctccgcga gtgacggttg cgcctgcctc
    30481 ctcgagcttc gcgaggactg cgtcgaggtg ctccgcgcgg gccccgcgcg cccggacccg
    30541 gccgccggtg atcgccccgg cgacgaggta ggtgccggcc tcgatgcggt ccggcagcac
    30601 gtcgtagctc gtggggtgca gccgctcgac accgtcgatc acgatggtgt ccgtgccgtg
    30661 gcccgacacc cgggccccca tcgaattcag gaactgcgcg aggtccacga tctcgggttc
    30721 gcgggccgcg ttctcgagga tcgtgcgccc ttccgccagg caggcggcca tcatcaggtt
    30781 ctccgtgccc gtgaccgtca cggtctcgag cacgatgcgc gcgcccttca ggcgtccggc
    30841 ccgcgccttg atgtagccgt tctcgatgtg gacgtcggcc cccatcgcct gcaggccggc
    30901 gacgtggata ttgacgggcc gggcaccgat cgcgcagcca cccggcagcg acacgtccgc
    30961 tgacccatgg cgcgccacca gcggcccgag caccaggatc gccgcgcgca tggtcttgac
    31021 cagttcgtac ggcgcgaacg tctcgcgcgt cgtgcgcgga tcgacctcga tgcgcatgcg
    31081 ctcgtccacc gtgacgctcg cgcccatgcg accgagcagc tcgatcatcg tcgtcacgtc
    31141 ctgcaggtgc ggcacgttgc cgatcgtgac cgggccgtct gccagcagcg cgcccgccag
    31201 gatcggcagc gtcgcgttct tggcgccgga gattcgcacc tcgccgtcca ggggaactcc
    31261 accgcggatc tgcaacttgt ccaaggtgtc gctccggtca gcccggtccg ggcgagccgc
    31321 gcgcggcctg ccactcggca ggcgtgtagg cctggatcga gagcgcgtgg atctcgcggc
    31381 ccatgcgcgg cccgagcgcc gcatacacga gctggtggcg ctggatcgac cgcttgccgt
    31441 cgaactgcgc ggcaacgatg acggcctcga aatgcgtgtc gtcgtcggtc aggacccgga
    31501 cctgcgcgcc ggacagcccc gactcgatca accgggtcac ctcgtctgcg gtcatgggcg
    31561 cagctcgctt ggggaaaatg ccagtttaac ccgccgtgcc gcgggccgtg gccccgtcgg
    31621 gatgcggcca gcccggcgca cctctagact atgcagcgtc cgccgaacgt caccccgcac
    31681 cccagtcacc caccttccgg ccacgccgct cacccatgcc cgccccggac ccttccatcg
    31741 cccgcggacg ccaggtcctc gagaccgagg ccggcgccgt cgcggccctc gcgagccgcc
    31801 tcggcgacga gttcgcgcgg gcctgcgggc tgctgctcgc ctgcaagggg cgcgtggtcg
    31861 tgaccggcat gggcaagtcg ggccacgtgg gcggcaagat cgccgccacg ctggccagca
    31921 cgggcagccc gtcgttcttc ctgcacccgg ccgaggcgag ccacggcgac atcggcatga
    31981 tcaccgcggg tgacgtggtg atcgcgttgt ccaactccgg ggagaccggg gagttgctca
    32041 ccatcctgcc gctgatcaag cgcctcggca ctccgctcat cgcgctgacc ggcaaccgca
    32101 actcgacgct ggggcgctac gcgacggtgg tgctggacgt gagcgttccg gccgaggcct
    32161 gcccgctcaa cctggcgccc acggccagca cgaccgcagc actcgcgatg ggagacgcgc
    32221 tcgccgtcgc ggtgctcgag gcgcgcggct tcacggagga ggattttgcg cgctcgcacc
    32281 cggccggcag cctcggccgg cggttgctgc tgctcgtcga ggaagtcatg cggcgcggcg
    32341 acgacctgcc cgccgtcgga ccggacaccc tgctcagcgc cgggctgctc gagatgagcc
    32401 gcaagggcct cggcatgacg acgatcgtcg attcgagtcg ccgcgtgctc gggatcttca
    32461 cggacggcga cctgcgccgg gcgctcgacc gacagatcga cgtgcacgcg acgccgatgc
    32521 gcgaggtcat gacggcgggc ggcaaggtcg cgaagccgcg catgctggcc gcggaggcgg
    32581 tgcgcatgat gcaggaccac cgcatcacgg ccctgcccgt cgtcgacgac gcgggcatcc
    32641 tggtcggcgc gctgaatgtg cacgacctgc tgcgcgccgg ggtgatgtga ccgtggatgc
    32701 cgtgctcgat cgcgcgcggc gcgtccgcct gctggtgctg gacgtggacg gcgtgctcac
    32761 cgacgggcgg ctctacatcg ccgcctcggg cgaggagttc aaggtattcc acgtgcgcga
    32821 cggcgccggc atcgtcgccg tgcagcgcgc cgggatcgcc gttgcgatca tttcgggccg
    32881 caactccgcc gcggtgtctc gccgcgcgga ggaactcggc atccgtcacg tccggcaggg
    32941 cgtggccgac aaggctgccg cgctgcgcac gctgctgggc gagctcggcg tcgcggcggc
    33001 ggaaaccgcg tgcgtcgggg acgacacgcc cgacctgccc atgctcgcgg cggccgggct
    33061 tgcgatcgcg gtggccgacg cgcaccctga ggtacggtcg gccgcgcatt gggtcacccg
    33121 agtcaacggg ggctgcgggg ccgtccgcga aacatgcgat ctcctgctca gcgccgctac
    33181 gtcctgacgc agcgcctggt cgcggtcgcc gcagtgaccg ccgccctcgc gctgctgtac
    33241 ggcctgttcg gtccggagcc gccggacgtc gagaagggtg cgacgccgcc gcagcagggc
    33301 tactacctgg tcgacacgac gatgacggag atgggcccga ccggccgccc gaaagtgatc
    33361 gtgcacgcgc ggcatgccga gcaacagctc gccgaccaga gcgtggacgc atcggacatc
    33421 acgctcgact cgccgacgga caagtacggc acctggcacg gcacggcact gcgcggacac
    33481 ctcacgcccg accgcaatac gatccagctc gcgggcgacg tgaccatgac gagccaactc
    33541 gagcagggcg tcgccgtgat ccacacggaa cacctcgatt acgacgtcga cgacagcgtg
    33601 atcaccacct cggacgcagt ggacgtgcgt ctcggagcgc acgtgctcaa ggcgcgcggg
    33661 ctccgcgccg atatgaacgc gcagaccctc aggctagaat cagacgtcaa tggccgcttc
    33721 actccctgac gtcgcgcgcg tccgcatcgc ggtcgcgctg ctctgctgtg ccgccgcgat
    33781 cacggcttcg ggggccgtgg gtgccgctga gtcgatcgag ttgccgcgac cgtccaagga
    33841 cctgccgatc gacctcgtgg ccgcgtcgtc cgacgtcgac tacaagaaca acgcgctgcg
    33901 attccggcag ctcgtggtca cgcagggccc gctccgggtc gaggcgcagg aagccacggc
    33961 gaccggcctc gacttcacga agtccgactg gacgctgacg ggccaggtca ggatcaccat
    34021 gcccgacggc aagctcacgg ccgatgacgc gcagctggcc ttccgcaaca acgagatcgc
    34081 gcgggccacg gcgcacggtg cgccggcgga attcgagcac aagctcaagg acagcccgca
    34141 ggtcgtgaag gggcacgccg ataccatcga ctacaacgtg caggccggca cggtgcgact
    34201 gtccggcgca ggatggctca ccgacggcga caacgtgatc cgcggggaca ccctcgtcta
    34261 cggtcttgca acccagcgcg tgcaggcgaa cccggacagc gcggtgcccg gcggcgtcca
    34321 catcacgatc aatcccaaga gccaggacaa gccgggcggg aagaccacgg accagcccaa
    34381 gggcaagccg gacgcgcacc catgagcgtg ttgcgcgccg agtccctggg caagcgctac
    34441 cgctcccgca aggtcgtctc cggcctgtcg ctcgacgtga gctccggcga agtcgtgggc
    34501 ctgctcgggc cgaacggcgc cggcaagacc acggccttct acatgatcgt gggcctgatc
    34561 ccctgcgacg aagggcgcat ccacctcgac gaccgcgagc tcacgcgcct gccgatgtat
    34621 cgccgcgcgc gcctcggcgt cggctacctg ccgcaggaag cgtcggtgtt ccgcaagctg
    34681 tcggtcgagg acaacatccg cgcgatcctc gaggtgcgcg aggacctcga cgacgaggcg
    34741 gcgcgcgagg ccgcgctcga tgcgctgctc gaggaactgc acatcgggca catccgcacc
    34801 aacgtcggca tgagcctgtc gggcggcgag cgccgccgcg tcgagatcgc gcgcgcgctc
    34861 gcggccgagc cgcgcttcat cctgctcgac gagccgttcg ccggcgtcga cccgatctcg
    34921 gtggacgaca tccagcgcat catccggcac ctggccgcgc gcaacatcgg ggtgctgatc
    34981 accgaccaca acgtgcgcga gaccctcggc atctgcaacc gtgcgtacat cgtcagcgcc
    35041 ggaagtgtga tcgcttccgg atccgcgcag gagatccttg ccaatcctca ggtgcgcgag
    35101 gtctacttgg gccaggattt caagctctag gaacgatgct caagccggcc ctgcagctca
    35161 agctcggtca gcagctgacg atgacgccgc agctgcagca ggccatacgg ctgctgcagc
    35221 ttccggtgct cgagctgcag gcccaggtgc aggaggcgct cgagagcaac gtcatgctcg
    35281 aggtcgagga ggaagaggag ccgatcgacc tcgcctcgct cgaccagccc gcgcccgccg
    35341 gcgaggaagg agctgaatcc gatacgggca gcgagcccgc cgacgaagtc gtcgtcgaga
    35401 tggaggatcc ctggtccgag gtgtccgcgg cgccgggcga gggccgctcc caggacgacg
    35461 acgaccggcc gctcgagttc acggacaacc gtgaactcaa cctccaccag cacctggtct
    35521 ggcagctcga ggtgagcaag ctcgacccgc gcgaggtctg gatcggcgag gcgctggtcg
    35581 atgcgctgaa cgacgacggg tatctcaccg agtccaccgc cgacatcgcg cgcagcctga
    35641 gcgccgacct gccggtgacc gaagccgacg tcgagcgggt gctggcattc ctgcagacgc
    35701 tggacccggc gggcgtgggg gcgcggtcgg tcgccgagtg cgtctgcctg cagctcggtc
    35761 agctcgatgc gggcacggcg ggacgggaac tcgcgctgcg gatcgccgcg cagcacctgc
    35821 aggaggtggc gaaccgcgac ctgccgggac tgcgccagag gctcggtgtc gcggaggacg
    35881 agttccacga ggcgctggcg ctgatccgtg cgtgcaatcc gcggccgggc gcggcgttcg
    35941 agagcgcacc gcccgaatac atcgtcccgg acgtgttcgt ccgccggacc cagcacggct
    36001 ggtcggtcga gctcaacccg gcgtcggtcc cgcgcctcaa ggtcaaccag ggctacgcga
    36061 gcctggtgtc gcgctcgcag gactacgcga cgctgcgcgc ccagctccag gaggcgcgct
    36121 ggctcatccg cagcctcgag atccgcggcg agacactgct caaggtcgcc cgctcgatcg
    36181 tgcagcgcca ggccgccttc ctcgagcagg gcgaggaagc catgcagccc atgatcctgc
    36241 gcgacgtggc cgaggcggtc ggcatgcacg agtcgacgat ctcgcgcgtg acgacgggca
    36301 agtacatgca cacgccgcgc ggcatcttcg agttccggtt cttcttctcg agccacgtga
    36361 gcggcgccgg cggcgaggac gtgtcgtcgg tcgcgatccg cgcgcgcatc cgcaagctga
    36421 tcgcggacga gcagccgggc aagccactga gcgacgcgca gctcgccgac gtcctcgcgc
    36481 gcgagggcgt caaggtcgcg cggcgcaccg tcgccaagta ccgcgaggcc ctgggactcg
    36541 cgtcgtccag tgagcggcgc cacgcggccg cgggctgaga tgcgcctcat catcgtgagt
    36601 ggcctgtcgg gctccggcaa gagcgtcgcg ctcaacatgc tggaggacct cggctactac
    36661 tgcgtggaca acatcccggc aggactgctg ccggcgttca tcgcctacac ggtccgcacc
    36721 agcgagccgt cctaccgcct gacggccgtc ggggtggatg cccgcaaccg gcccgaggac
    36781 ctggccgacg tgccgcggct ggtcgccgaa ctgcggcgca gcggcatcgg ctgcgagctg
    36841 ctgttcctgc gcgcgggcaa cgacgtgctg ctcaagcggt tcagcgagac gcgccggcgc
    36901 cacccgctga gccgtgaagg cctcggcctg caggaggcgc tggagcagga gcagcggctg
    36961 cttgcgccgg tcgcgaccgc ggccgatctc acggtcgaca cgtcgagcct gtcgatgcac
    37021 gagctgcgcg acatgatccg cgaacgcgtg gtggagcggc cagccgccgg gccgtcgctg
    37081 ctgttcgagt ccttcgcgta ccgcaacggc gtgcccgacg acgcggactt cgtgttcgac
    37141 gcgcggtcgc tgccgaaccc gtactgggag ccggcgctgc gcgacttcac cggtcgcgac
    37201 gagccggtcc ggcaattcct cagccggcag gaggaagtcg cgcgattcca ggcggacgtg
    37261 acggcattcc tcgagcgctg gatgccgtcg ctcgcacgct cgaaccgcag ctacctgacg
    37321 atcgccgtcg gctgcacggg cggccagcac cgctcggtgt acctcgccga gcagctcgcg
    37381 gcgcatttca acgcgagcct ggggcaggcg ctggtgcgcc accgcgacct cgcgggccgt
    37441 cggcacgaat agtcgcgcga cgcgagctgg cgtcgccgag cacgcagtcc gcgcggacag
    37501 ccggcatggg ccggccgagg ggaggtcttc cgcgctccgg gtctaccgcg cttcgaaaac
    37561 tccggccggg gacggctcgg agttttcggc gacccttgcg ccgtcgggtc cgcggcaaaa
    37621 tttcggcggt gactgccgac ccatcggccc agccgcggac ctgcgacgtc gctgcggcgt
    37681 ccgacacgga gcgcggaaga cctcccctcg gccggcctcg cattcctgct ccgcgttcgc
    37741 gctgcatcgc ggtcgcgcaa tgcgcctcgc cgcggcgact gcgacgtcgt aaagtgcgcc
    37801 ggccatgaac gcggaactcc accccatgcg gatgagccgg ctcgcaacgc tgaagcgggc
    37861 gctcgagcag ggcaccggcc gccaggtcca gcgcctggtg aactcgctgc atccggccga
    37921 ggtggcgtcg ctgctcgagt cgctgccgca cgcgcagcgc gacctcgtgt gggacctcgt
    37981 cgacccggaa ctcgagggcg acgtgctcgt cgagctgaac gacgaggtcc gcgccgacct
    38041 gatccaggag atgggcaccg aggaactggt cgcggcgacc gagggcctcg aggtcgacga
    38101 cctcgcggac ctgctcgctg acctgccgga gacggtcaac cagcaggtgc tgcggtccat
    38161 ggactcgcag gaccgcgagc gcctcaaggc cgtgctcgcc tacgacgccg acacggccgg
    38221 cggcctgatg aacaccgaca cggtcagcgt ccgcccggac gtgacgctcg agaccgtgct
    38281 gcgctacgtg cggatgcgtg gcgagctgcc cgagcgcacc gactcgctgt acgtcgtgaa
    38341 tcgccacgac cgctacctcg gcacgctcgc gctgccgcgg atcctgaccg aggatcccga
    38401 tcgcaccgtc ggcgaggtca tggacgcggc ggccgaggcg atcgctcccg agacaccggc
    38461 gagccaggtc gcaagcctgt tccaggatca cgacctgctc tcggcgccgg tggtgggacc
    38521 ggagggacgc ctgctcggtc gcatcaccgt cgacgacgtc gtggacgtca tccgcgacga
    38581 ggccgaccac tccgtgatga gcatggccgg cctcgacgag gacgccgaca tgttcgccgg
    38641 ggtcgtgccg agcgcgcggc gccgcggcgt gtggctcggc atcaacctcg ccaccgcctt
    38701 cctcgcggcg tgggtcgtgg gcctgttcga atctacgatc cagagcatcg tcgcactcgc
    38761 ggtgctgatg ccgatcgtgg ccagcatggg cggcgtggcg gccacccaga cactgacgct
    38821 gatcgtgcgc gggctcgcgc tcggccaggt ggagcgagcc aacgcacgct ggctcatggc
    38881 gaaggagatc gcggtcgcgc tcctgaacgg gctggcctgg gccacggtcg tcgcggtcgt
    38941 cgcgctggcc tggttccgca actggaagat cgcagccgtg atct
//
LOCUS       DAOMFD010000004        32588 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_177_length_32588_cov_34.793441, whole genome shotgun sequence.
ACCESSION   DAOMFD010000004 DAOMFD010000000
VERSION     DAOMFD010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 32588)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 32588)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32588
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_177_length_32588_cov_34.793441"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..570
                     /locus_tag="PL152_00575"
     CDS             <1..570
                     /locus_tag="PL152_00575"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQR47793.1"
                     /translation="APGPDNLMVLALGASRGRTRGMAFGLGCALGCLSHTVLAAIGVG
                     AVIAASPMAFTVLKVLGGLYLLRLGWQALRSGGSIRPSEAGETVESLLRMFSRGLVAN
                     AINPKVVMFFLAFLPQFVVASRGDTAWQIAQLGLVFTAQAAVIFGLLGYFAGAIGQWL
                     GRHERAGLWLDRVAGTVLIALGLKLLVSR"
     gene            1022..1222
                     /locus_tag="PL152_00580"
     CDS             1022..1222
                     /locus_tag="PL152_00580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47794.1"
                     /translation="MTPRSIREFAAIDPGREAIPGETTVCRFRLPLERHQLGPQILAT
                     VNAHLKRSGIKISAGAIVDVLA"
     gene            complement(1580..1888)
                     /locus_tag="PL152_00585"
     CDS             complement(1580..1888)
                     /locus_tag="PL152_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47795.1"
                     /translation="MFTRIAPQRARDTDGAIVQVGSRDRIEYARGEITAVLDADFALV
                     TGIYPDSLTIRKAGGALATISDEEREAIFCKIKEGLDCLGIRYEECRRGSQGRPPSDT
                     "
     gene            complement(1888..8280)
                     /locus_tag="PL152_00590"
     CDS             complement(1888..8280)
                     /locus_tag="PL152_00590"
                     /inference="COORDINATES: protein motif:HMM:NF015497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HQR47796.1"
                     /translation="MRNARAVQRLARDTRIDASPGSRSADSIRILATAAAACALTLLA
                     GAGWAIETQSPSPDGFVVEAPTDLPSSDVIGVEPEPIVPPDPVPTQPLASVAAATAAG
                     RTPGSFGVTSSGAATYRIPIWTPPGVGSVGLDLALVYNSRAGNGVLGQGWALSGLSVI
                     TRCNRTIAQDGAPAAVGNALADRYCLDGRQLKLVSGSYGAPDSVYATEVESFSKIVAS
                     GIAGNGPASFTVTSKNGLVYEFGTASDSQVYAGSSGTVRTWALSRIRDRAATTSGNSI
                     AISYANDARNGAYTNGTIRVSSITYPVTATGQGPFYRVSFAYSARPASDIVAGYLAGS
                     VVREPNQLDTITVASVSSGATIKSYRLSYAQGPATGRLQLRSVQECSPTSCLAPTVVT
                     YQQGATGWASTAASTGAVASASAATRAVDLNGDGLSDLVLPRAVSSGSLGWWIALARP
                     GGYASPVDTGVTSTVAAHVITGRFLGNGRTQVLVEQSGSWVLVACEGTAIRLTPVAIV
                     PDGEYLAADVDGDGLDDLLSHVSSSPATLQGRRNVTVPGVGASVAFAASRVTLWTVSG
                     GYVSPRSYNNVADINGDGRADLAVHMVATVKGGASRLTPLLSNGFDAPFTEGAQRDFW
                     PDGRIVIVDWNGDGCSDFLQVSSVLVSNCNGEFTEIPTGAVGMARDANGNATVLPADV
                     DGDGRTDLLYVKAATSSWFSNTWYVVRSTGIGAAAPVAIGMPAPDKTAWFVVDADGDG
                     KTDLAWRDDARGGVIKYNLHNAPGVPADLATSFSDGFGMSQGASYVSIASGNYTKHAD
                     AAFPEVDYAGPLYVVSQVAASDGTGGTYQNLFQYFGARVHLQGRGFEGFGSRRSYDSR
                     SGLYTYDYFERAFPYTGLHTRRVVLQSNLASKVSEWSATPAWQSLGAGFEQRAFPHLA
                     SAVETRYEAGGALDGQAVTQATETYAYGDGYGNRTQVQRVVTDKDPASPFSGSSWQSI
                     TVTTYANDATSNCLGLPVSSVVTRTIPGQPSQARTVAYTVDTGACRIWQQVLEPATPS
                     LKVTTTLAFDGCGNVSSVQVVGSASTGAAMPARTTRFDYGPRCQLPETLTNALGQATR
                     VSYQYDFGVPVSVTDPNGVTTTWQYDDFGRRVLETRPDQTTTSWAFESCSTGPCWGQG
                     DLRFHVYETSRGSAGEVYDQREQLFDGFERPRSSQYMRALGAWVAETWKYDSLGRPVT
                     RYRPTSGPANGYVGWTYDVLDRVTARKLYQPNGAVDRASSFAYAGRTMRATDALGRTR
                     TRVVDVEGRLRRVVDPPPGGTTSYDYDAFGNLNRVQDPIGAVSAGWYNLRGFRTQWAD
                     ADRGTWTFAADSLNELVGWTDARGQSFSVAYDALGRPVARTEPDGTSTWTWGASAAAH
                     NIGRLQSAAGYGYAENLAYDGAGRVASRTIVTDQSYQYDFAYNSAGALDTLTYPSSPT
                     PTGQAGARFKIRYAYSYGEPVQVTDVTDAPASTRTLWTLNQANDDSLPTLETLGAGLL
                     RVASGYKAWTGEIVSRQSGVLPATGNRQNLAYQWDTAGNLTQRQDLNQGLTETFTVDA
                     LDRVQSSTLNGVANFAAAYDASGNVTSRSDVGNYTYGSAAHPHAVTAAGSHTYAYDAN
                     GNQVTRDGASQAWASFNLPVQLAQPIDGVTYQSQFSYGPDHRRWKQVASYANGTETTY
                     YVGGLLEKESTTSTGRTYWRHYAYTPSGVAIVVSRNSDGTGSTSYALADHLGGTDTVL
                     DASGSVTARTSFDAFGGRRGGDWSSGTTPDWAGIANSTRHGYTGHEHLDNVALIHMNG
                     RVYDPGTGRFLSVDPVIGDLGNSQTVNPYAYAGNRPLVLTDPSGLDPTGGVLTVSVVG
                     SVIKTVGDLLFGGGDRYVPPASAMPGQSAQTGGVMCGPGQSTVACGGWVLSAAGSPIL
                     RSGAPTSSWVNETGADDPYAGEILEQLFIDLGNNAVDVLILAPMYDAIDAYDSARDRR
                     YGTAVLDAGLAICGVSKVCEGLAAGAIPLARVAGNLARARQVSGSKAAFNRLMRRAET
                     LDIRTARNGAVFYSGPGNRALAEEFATANGRSTLEMTPGGNWLDQQQLFGPNSPISAE
                     QAVQVWSRVSTRFADGASGSVFGFVSGTRVSSIFSTVEYPALLRNPEIVNVITGGH"
     gene            complement(8530..9543)
                     /locus_tag="PL152_00595"
     CDS             complement(8530..9543)
                     /locus_tag="PL152_00595"
                     /inference="COORDINATES: protein motif:HMM:NF022825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3187 family protein"
                     /protein_id="HQR47797.1"
                     /translation="MNSTPRIRPCGSCIRGAVLAAATLALAGAAAQSSQAEPLITRNQ
                     NPLTLPYGLPMPLPARLTDAGHGRAALDVNWSNSASLDSSGQDDFTLDAETVDVRLRL
                     EYSPWQDWSLMVELPWRDLSGGSLDGFIDNWHDFWNLPQGPRKNMPKDRLLIDYQRDG
                     EVLLHVDDSGSGIADIPIRTGYQLMSNEKSALSAWLTVDLPTGDSDYLLGSGGTDVAL
                     SVAGQTQLAEKWQLFGQVDAVYLGNGDILPGEQENFVVAGLAGVTWNAWRRLDLTVQF
                     YANSKVFDVPQNGLSGDAIVLSYGGTWRTEHGWRFDLGMNEDIQVEASPDATFYFLVQ
                     KEF"
     gene            complement(9662..10714)
                     /locus_tag="PL152_00600"
     CDS             complement(9662..10714)
                     /locus_tag="PL152_00600"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HQR47798.1"
                     /translation="MKAMLLRELGPVVAGRALLEFADVPVPEPAPGEVLLRLAACGVC
                     HTELDEIEGRTAPPRLPVIPGHQAVGRIIEYGDSVPGTDLPISARPRIEDRVGVAWIF
                     SACGHCEQCTAGNENLCPGFRATGRDVNGGYAEYMTAPAEFVHPIPDAISDIDAAPLL
                     CAGAIGYRSLRLAGVRDGMRLGLTGFGGSGHLVLKMVRHRYPSARVYVFARHPEERAF
                     ALELGATWAGNTTDRAPVKLHAIIDTTPAWLPVLGALEQLEPGGRLVVNAIRKEDADK
                     GHLLDLDYSRHLWLEKEVRSVANVARVDVREFLALAAEIPLRPEVETYDLRDAGRALL
                     DLKTQRVRGAKVLRLA"
     gene            complement(10753..11862)
                     /locus_tag="PL152_00605"
     CDS             complement(10753..11862)
                     /locus_tag="PL152_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007612981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0051903 - S-(hydroxymethyl)glutathione
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA];
                     GO_process: GO:0009404 - toxin metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase/class
                     III alcohol dehydrogenase"
                     /protein_id="HQR47799.1"
                     /translation="MKTRAAVAFEARRPLEIVELDLDGPKSGEVLIEIKATGICHTDA
                     YTLDGFDSEGIFPSVLGHEGAGVVVEIGPGVTSVKPGDHVIPLYTPECRQCKSCLSRK
                     TNLCTAIRATQGKGVMPDGTTRFSLKGRPIFHYMGCSTFSNYTVLPEIAVAKIRDDAP
                     FDKACYVGCGVTTGVGAVVNTAKVEAGANCVVFGLGGIGLNVVQGLRMVGAAKIVGVD
                     VNDAKESWGRRFGITHFVNPKRVEGDLVQHLVTLTDGGADYTFDCTGNVAVMRQALEA
                     CHRGWGTSVIIGVAEAGKEISTRPFQLVTGRVWKGSAFGGARGRTDTPRIVDWYMEGR
                     IQIDPMITHTMPLERINDAFDLMHRGESIRSVVIY"
     gene            12039..13394
                     /locus_tag="PL152_00610"
     CDS             12039..13394
                     /locus_tag="PL152_00610"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR47800.1"
                     /translation="MNGETQVSRTTVDEWAWDPATNPLPASVDVAIIGGGIVGCCAAW
                     FLAREGLSVGLFEKGRIAGEQSGRNWGWVRQQGRSPVELPMMMRSRRLWPELQRELGE
                     DLGFRQGGCMYLAATQVQLDVISPWLVVAREHGLDTRLLTRSELGGVLRGDLTKWAGA
                     LYTASDGRAEPNRAAPAIARGARRAGAHIITGCAVRGLQTAAGRVQGIVTEKGAVSAR
                     SVVCAAGAWTRLFCRSLGITVPQLMVLGTVARAAPSDAVLEGEVWAPTLGIRRRADGG
                     HTIAHGASFLHRLTPDTLRFATKFTGLYRGTHSMRVRAGAEFFRDLAMPARWDLDAPS
                     PFERQRMLDPAPETGVLRQIRGELRQWFPEIADVPFVETWAGMIEASPDALPIICESD
                     RLPGFHIATGFSGHGFGIGPGAGRFVADLVQGKADPTELAAFRLNRYFDGTPIRPGPS
                     L"
     gene            13503..15419
                     /locus_tag="PL152_00615"
     CDS             13503..15419
                     /locus_tag="PL152_00615"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR47801.1"
                     /translation="MPRVAAYYVAGAWVAAQAASLLLDAFDLGHYTRYVIVALGAAFP
                     VALVLAWRFDITPRGIERTLDRAVPAAPASQAPAPERSIAVLPLANLSDDPANEYFSD
                     GLSEEIRNQLARVPGLQVAARRSSFAFKASAVDVREIGRRLNVATLLEGGVRKHAGTV
                     RIDVQLVSTRDGFHLWSQGFERRLEDVFRLQTEVAAAVLEAVRARHGLEECTLPAPDE
                     RRFEVYNAYLLGRHHFHRRSEQSLQKAAQCFEQAIALDPGYALAYSGLADTYLLLGGR
                     YYGSATTADSVARAEPCARKALELAPALAEAHASLGLVQLNRGEAALAASSLARALEL
                     NPGYTMAHVWYGLALTAQGRYRDAATSNLEAFRLDPLSPIVNTNVGFDALRFGDYAEA
                     EARFRTAIEVDPGFAVPYSGLSKMAMDRGDTGTAHRWIDAAIEHAPTRAFYRSRKALL
                     SLQSGDLDAAAEHAAEANRLAPHDVLNCDFQVALHMARADRDAIARIASGGCGPDYST
                     GAVAQACIALGQLDVARELYEHDVPDIDRMIHEILNDDWTWRLPHPINHAHLLLPLDA
                     ARAGAGLNAVLAACDRVFADGIVNGSLTYLAATALAVLGRSEAAQGKLAEARRLGWSH
                     DWWRRVDWNARGLD"
     gene            complement(15456..16142)
                     /locus_tag="PL152_00620"
     CDS             complement(15456..16142)
                     /locus_tag="PL152_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQR47802.1"
                     /translation="MLTVHHLETSRSQRVLWLLEELGTPYELKVYKRTAQRLAPPELR
                     KVHPLGKSPVITDDGKVIAESAVIIEYIAETYGSFAGGELAGLVPERGSAEDWQCRYW
                     MHYAEGSLMNWLVMKLVFGSLPKQPMPFFARPIARAICNKVQGQLIDPNLATAASYVD
                     GHLGRNRWFAGPLLTVADFQMSFAVEALLSRASYAARLPHLVEYRSRINARPAYQRAL
                     QKGGPVIMAS"
     gene            complement(16214..16750)
                     /locus_tag="PL152_00625"
     CDS             complement(16214..16750)
                     /locus_tag="PL152_00625"
                     /inference="COORDINATES: protein motif:HMM:NF020918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HQR47803.1"
                     /translation="MNEFLEEQTRGLKEMAENLRKSQLETARKAALRSAARVRSLNAR
                     AREMARSGVKLSNISHGTLERLIGLHEEIVTAALAEAATQIEKLAYTESARDFGRDQA
                     RVLQSARERIVNDVSRGMKILREAAEQAREVGARAAKAAPAARKPAARKAKAKAKARK
                     ATRKTAARAKKAKTRRAR"
     gene            17008..>18704
                     /locus_tag="PL152_00630"
     CDS             17008..>18704
                     /locus_tag="PL152_00630"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HQR47804.1"
                     /translation="MAHDVFLSHAAADRAAALAVLEGLEAAGIRCWIAPRDIPAGSEY
                     GQQIVDAVKGCRAFVVIFSANANASPHVRREVERAVSTDRPIVPFRIENVTPTGAMEY
                     CLGGTHWFDALTTPLEPHVANLIATLKQMLGAAGRTAEGKASQEAAERPSIAVLPFNN
                     MSGDAEQEYFSDGITEDIITDLSKVSGLFVVARNTAFTYKGKPVKVQEVCRELGVKYV
                     LEGSVRKAGSRVRVTGQLINGEDGGHVWADRYDRDLTDIFAIQDEITHAIVEQLKVKL
                     LPQEKRSIAQAPTDNIEAYTYYLRGRQFLHRHTRSYYQFARRMFVKAVELDPAYARAY
                     AGIADCDSFLYLHYDIGIAIDDILATSARALELDPGLAEAHASRGLALSLAQRYDEAM
                     AEFERAIALDPNSYEANYFYARACFAQGKLPQAAQLLERSAGLKPDDYQAPAMLVMVY
                     RSIGREADVPGAARKVVELSEAELGRHPENPRPAYLGAGSLIEIGEIDRAKEWLSRAV
                     ATDPDDVLTQYNVACIYSKLGEYDAAFDLLERLLPQANHETRSWVRHDADFDPLRTLP
                     "
     assembly_gap    18705..18804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18951..19595)
                     /locus_tag="PL152_00635"
     CDS             complement(18951..19595)
                     /locus_tag="PL152_00635"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016471 - vacuolar proton-transporting
                     V-type ATPase complex [Evidence IEA];
                     GO_function: GO:0008553 - P-type proton-exporting
                     transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQR47805.1"
                     /translation="MIHPTRTDLRQLREKAASVANSVAILRARRQVLIRGFLESIRPV
                     VRSRDAIRRDYLRALAELRLSEGHEGTAGIESLAALAERPVGVDVEERNALGVRYREL
                     TVFGPFVRRPEERGYDYTVTTPHLDEATHLFESIIEAMLGSAAFESKLKMLGEEILRV
                     TRRTRVLEERLLPRLRADIRAIVQFLGEREREAHYRLKMFKSARDTAARGVVRK"
     gene            complement(19592..20932)
                     /locus_tag="PL152_00640"
     CDS             complement(19592..20932)
                     /locus_tag="PL152_00640"
                     /inference="COORDINATES: protein motif:HMM:NF003235.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HQR47806.1"
                     /translation="MRLSQHSYGTLQSIQGPLVFVDAVLESRMGEVVTIVFPDGHEAE
                     GEVLRIDGDTVLIQVIGDSRGLDLDHSRVVFTDVVKRAPLSLAVLNRSFGGSFQPIDD
                     LPMFLPEKWAPVAGFPLNPTARERPQEMIETGLSCIDGLNTLVKGQKLPIFSCAGLPA
                     GEIAARILQQAQTSGAGAQAGARSTVVFVALGLTYHEYAFYRRTLEQVRGDFVAFINL
                     AKDPVVERLLAPRFGLTVAEYLAFEHGRDVLVVVTDMTHYCDALREVSTAREELPGRR
                     GYPGYLYSDLASIYERAGRIKDCRGSVTMLPVVTMPEDDITHPIPDLTGYITEGQIVL
                     SRELHQRGIFPPVDVLPSLSRLMQRGIGKGRTREDHRDVANALYMHYANGRDLRRLEA
                     IVGREGLGDADKRMLDFADAFEREVVAQGQARRTIVETLDLSRALLERFGLGLP"
     gene            complement(20913..22418)
                     /locus_tag="PL152_00645"
     CDS             complement(20913..22418)
                     /locus_tag="PL152_00645"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF003220.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HQR47807.1"
                     /translation="MAGRIVGISGPTVTVELPGLRLYERVTVGHAALMGEVVRLERGR
                     AIVQLYESTRGLGIGEPVVGTGSQLTVRLWPGLLGHMFDGLQRPLEEMRRTQGPFLRP
                     GAADAAHGPGLSPRMPTEPPQEQAVEEWPVRLPRPYRRKLSTFEPLVTGQRCIDFLFP
                     LARGGSAIFPGGFGTGKTLLEQSIAKYADVDIVVYVGCGERGNEMAEMLDDFSVLEDP
                     WRKRPLMERTIVVVNTSNMPVAAREASIYTAVTMAEYFRDQGQHVLLLADSLSRWAEA
                     LREISSALEEMPGEEGYPTYLSSRLAEFFARAGVVETLDGRIGSLTVLLSISPPGGDF
                     TEPVTQACLRNTGAFFMLDTSLAHRRHFPAIHWFQSFSLYEAEVVAHLEKQVAPDWGA
                     LRSRARVLLQREESLREVKEIVGAEGLQDADRLLMRTTEAVRQRFLAQNAYTDDAFST
                     PQQTHASIRALLEDYDRAAARLEKGESLQEILKDDRASAAHGGEPHASQPA"
     gene            complement(22418..22735)
                     /locus_tag="PL152_00650"
     CDS             complement(22418..22735)
                     /locus_tag="PL152_00650"
                     /inference="COORDINATES: protein motif:HMM:NF014092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HQR47808.1"
                     /translation="MKHVIVITPQDANPGFALAGVRQAATSTEGLPAALAAVAREPLT
                     GVVIVDERLVDAAARDALRDMDRRWPGLVIVLPAPMPGERAEEDYVLQLIRRAIGYQV
                     RLS"
     gene            complement(22732..23667)
                     /locus_tag="PL152_00655"
     CDS             complement(22732..23667)
                     /locus_tag="PL152_00655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47809.1"
                     /translation="MTLPLRDIAGEGYPTDYLLARVRARRATLAAAWARVRATGRDDA
                     ASDEAIWEGFLHELGWLRAQMNRRLRDSLAGVFLLFEVKTIVLCLRARAAERSAEVQR
                     LLADSQLDDVVRRGLLLGPDIAASVAEVAAGWTGEAGVSAALERAYAEAGLKGFEQRL
                     ARDQLERVTAGQLPTEVRRFFVAFIDVRNLVTLYKHLRWACGDATDFVAGGSVECSRL
                     GAASSSKDTATLDALVREIAGRAAPPVSIDEAALETILLGSLTARVRAIARDSAAVGP
                     ILEYMWRCYVHARNRSILFHAAGFDAAVLDQELIA"
     gene            complement(23648..24232)
                     /locus_tag="PL152_00660"
     CDS             complement(23648..24232)
                     /locus_tag="PL152_00660"
                     /inference="COORDINATES: protein motif:HMM:NF014093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033178 - proton-transporting two-sector
                     ATPase complex, catalytic domain [Evidence IEA];
                     GO_function: GO:0046961 - proton-transporting ATPase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit E family protein"
                     /protein_id="HQR47810.1"
                     /translation="MGREALLESLRAKAAEDREAVWADARAAAERHRVELARVAEAER
                     ESASQAAAGLEQALASEAVVDARRVARERRARAALALAERCRHLAMAELPRLREEGGP
                     PLFTALAQELPGLAWDRVSVNPGDADLAHTSFPHAEVAGDPAISGGMVLEGDAGRVRI
                     SNTLDTRLATAWPDILPQLVGGLLPEQRDDTAAP"
     gene            complement(24236..24469)
                     /locus_tag="PL152_00665"
     CDS             complement(24236..24469)
                     /locus_tag="PL152_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47811.1"
                     /translation="MDAAMHWDKILMAFAAALALGVSALSTAWVQSRIGAAGAGALAE
                     KPELTATIIILLAIPETIVILGFVIAAMILLQG"
     gene            complement(24481..26364)
                     /locus_tag="PL152_00670"
     CDS             complement(24481..26364)
                     /locus_tag="PL152_00670"
                     /inference="COORDINATES: protein motif:HMM:NF013647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033179 - proton-transporting V-type
                     ATPase, V0 domain [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase 116kDa subunit family protein"
                     /protein_id="HQR47812.1"
                     /translation="MLVRMSKVEITGPRGQLLPVLETIQKSEVLQIDPDIRHRIDLGS
                     DPVSQVRPLALDGKTLAERLFLEDLNLKVERLLALLPKVDTRESYLSPSRAVSSIASV
                     VGKHLDAAEERRRRRESLQGELAQVNRYLMFLTTVDSLAPKGAASAGLDFIGVEVRDP
                     AALEQLTRVAGRVLLGAEVRTAQAKDGSYIGLLTTEKDLAAKLKEGLRENHIPEIALP
                     AYLEGLALPDKLKALQRKRDELSAGIDSLDREEGEFATAWRGHYLSMRSWLQRRLALL
                     RTSASVYETDRCFVIYGWMPSAGLDDLRRTLADRHGDTVVVEEREMLKKDLDDVPVAF
                     RNPPYFRPFELLVRLLPTPRYTSIDPTPFIGIFFPLFFGMILGDVGYGVLLTIAAAVL
                     IAMGRKRPALRPIGQILLAASIYTVIFGALYGECFGDLGVRLLGIHPFIDRRTSIIPM
                     FYFALAVGGVHVLVGLVLGVIAAIRGRHPKEAVFRGISILMLLLVAGLLASYMAPVAG
                     LLRKPMLVAILIAAPVVLLTGGVLAPFELLRTLGNIVSYARIMAVGLASVLLAYVANS
                     LAGAAGSLWVGIAVAVLLHAFNIVLGVFAPTVHALRLHYVEFFSKFIETGGRRYQPLR
                     KAD"
     gene            complement(26354..26683)
                     /locus_tag="PL152_00675"
     CDS             complement(26354..26683)
                     /locus_tag="PL152_00675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47813.1"
                     /translation="MEHSTLQDILGVEREIREQLDAERTRASQWLEDARREIDAAHRK
                     ELESVRQEAERRREAVRRAAAERADRHAREAEALALAVDRRSDEELRRAVRECIAVIV
                     PGTSRAR"
     gene            26941..27231
                     /locus_tag="PL152_00680"
     CDS             26941..27231
                     /locus_tag="PL152_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47814.1"
                     /translation="MPKFRPSGRVLTVAAAAALLLASNAYADVVTGTVAPQGASVVIV
                     DSAGKTVAKLPAGPYQLQLPVGKYIARCQAPKQRDQEFLSLSDPVTVDINCG"
     gene            27242..28294
                     /locus_tag="PL152_00685"
     CDS             27242..28294
                     /locus_tag="PL152_00685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47815.1"
                     /translation="MTDTAAPRPPEGNKPPATHSGPGWRANIRLAADVVNTLAIPLAT
                     LGATIVGVHLTNSLKEREIAIATENNVQALRNQREESETALRSAMFRELVGPLLQSEA
                     DSRGSDAKSDLPRAQRLALLAELLTLNFHEHFELGPLLRYVDTLPGQTDDARMRLRST
                     ARRVISRQLAPMLAVSDGAGQHAGFQEISLDADVSAPPDALLSCAIPESAAQASGALT
                     LDCSLAAQGSPLRVTSPDGRDHLQISFRGVSWRDQTVGVYALVTDAKGVREAGTPPIE
                     FTLSPYSLPYSDNTLLSSGNRFGVYLTRVDVIPDVARIMRLSFVWFPRDFVPPRERPM
                     DLDINRPDTKAEATKG"
     gene            complement(28352..29806)
                     /locus_tag="PL152_00690"
     CDS             complement(28352..29806)
                     /locus_tag="PL152_00690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015333 - peptide:proton symporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide:H+ symporter"
                     /protein_id="HQR47816.1"
                     /translation="MRPIAARLSRIPEGTAALFFIQVFATLGFAVLYSTLVLYTTRHL
                     AFPVRDATAVMGMFGAFNYGLHLFGGYLGGRFLSNRNLFVGGMVLQVLGCATIALGTT
                     TALYWGLGLFLTGSGLNVTCLNMMLTQRFAPADNRRETAFLWNYAGMNVGFFVGFAVA
                     GHYQLTENYTSLFLFATIGNFLAIVLAGVNWKALRDLDTPLLGATAPQFRKRFAIGVL
                     VLVGVPPLVCIMLQHAGPTGAALKAFCALVAGWLIYLTARHTDLVEKNNMKAYLVLAV
                     GSLVFWSLYQMAPNGLQLFAVNNVDLHVWGIAVAPQWVQNINTVVIAVGGPLLAVLFG
                     RLRARGWVIDLPRQFAASLILMGLGFLALPVGISFADSAGKSAFFWLFLSYVLQSIGE
                     LLISPIGYAMIGKLAPRQYQGIMMGSWMLITGLASLFAGDFSGMVPEPAGTTAVATNP
                     EYASLFARLGWGSTAVGVVLALLIPVLRRLIRDK"
     gene            29962..31212
                     /locus_tag="PL152_00695"
     CDS             29962..31212
                     /locus_tag="PL152_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR47817.1"
                     /translation="MYIGTHGKDEVFRLFGQHVSSGKARFFSSVGIDFVLGRREGPWL
                     WDITGARKLIDTHCNGGVFNLGHRHPEIVATMRAALDELDIGNHHLVSEQRAALAARI
                     AALMPGDLSYTVFGVSGGESVDLAIKVARAHTGRSKIVSAAGAYHGHTGLALAAGDEK
                     FRAPFGPQAPGFVQVPFDDLAALDRAVDDTTAAVILETIPATFGVCVPADGYLAGVRA
                     LCDRRGVLWIADEVQTGLGRTGRMWGFEHDGVVPDIVVLGKGLSGGIFPMTATCFRAP
                     IEHVFHADPFIHVSTFGGAELGCPVAAHVLQVASDPAFLQNVRDVAAVFAEGFRGLAT
                     KHEVLVRLRQRGLMMGIEMVNELCGPVFTKAAFDHGLLSIYAANDRRVAQLLPPLTTD
                     RSLAEEILRRVDAALGVVGALLRR"
     gene            31209..32180
                     /locus_tag="PL152_00700"
     CDS             31209..32180
                     /locus_tag="PL152_00700"
                     /inference="COORDINATES: protein motif:HMM:NF039685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6206 family protein"
                     /protein_id="HQR47818.1"
                     /translation="MTDLSVDSARLDAFEHDLDLRNPERGPAATKVLGYGEISTVLAI
                     DAPGMTQLAFKRMPMFLDEGEAARYEALHAEYLELLNERVGLRVVPGRTVRLGRHRGK
                     IVMYIVQERLAPDRVAQRLIPRLSVAGLAALTRSALTEAGKVYAFNASHAPAVEVAVD
                     GQVSNWALREASDVDRLERGDTVRLDYLDTSTPLLRKGGREQLDTELFLRSAPSFLVP
                     LLRAFIVQDVVDRYYDFRRVAIDAVANHYKEQRADAVPTLVDTVNRFFAANVPGVEPI
                     SEKAVRDYYRTDAFIWRVYLAARKLDRKLHRLSGRDYPYILPAKVRR"
     gene            complement(32190..>32588)
                     /locus_tag="PL152_00705"
     CDS             complement(32190..>32588)
                     /locus_tag="PL152_00705"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQR47819.1"
                     /translation="AVAEKGGVCGIYVMPYLSKGRQPTAADVVRHVEHALDVCGEDHV
                     SIGTDGTLSAVDLTPEFKADFRDNVRERRARGIAAPDESEEGYLFASDLNTPRRFETL
                     AGLLLARGHSESCVEKLIGANLLRVFGDAW"
BASE COUNT         5098 a        11319 c        10875 g         5196 t
ORIGIN      
        1 gcgccgggcc ccgacaacct gatggtgctg gcgctgggcg cgtcgcgcgg gcggactcgc
       61 ggcatggcgt tcggactcgg ttgcgctctc ggatgcctga gccacacggt gctcgcggcg
      121 atcggcgtcg gtgcggtgat cgcggcatcg ccgatggcgt tcaccgtgct caaggtcctg
      181 ggcgggctgt acttgctgcg cctcggctgg caggcgctgc gcagtggtgg gtcaatccgg
      241 ccgagcgagg ccggcgagac ggtcgaatcg ctgctcagga tgttctcgcg cggcctcgtc
      301 gccaacgcca tcaacccgaa ggtcgtgatg ttcttcctcg ccttcctgcc gcaattcgtc
      361 gtggcgagtc ggggggacac cgcgtggcaa atcgctcagc tcggcctggt cttcacggcg
      421 caggcggccg tcatcttcgg gctgctgggt tattttgctg gcgccatcgg gcagtggctc
      481 ggccggcatg aaagggccgg gctgtggctc gaccgtgtcg ccggcaccgt gttgatcgcg
      541 ctgggactaa agctgctcgt gtcgcgctga gcgcaggcat cggagcgcgg ctcgtgccta
      601 gtcaatccgg gccggtcttc cttcaaccgg ctctcgggcg aattcgctgc cggcgcgacc
      661 gttcgaccat cgagagccac acgtcgggcg gattccagcc gacgctgttt ctctccatac
      721 actgagctgg cgaccggcga tcgactgtcg tgccgtcgtt ccgtgatgga tccagagggt
      781 gggcccgccg aatccatgac tcggatgcgt tcgtttccgc tgtgccgttc atgcgcgtgt
      841 cctccttcgg gcacgcatga tgcgggggga ttgttactgg ggagtgactg ggtgatcctt
      901 cccaaactag acagcggcaa tcaatcgact tgtcgcatcc ctaagggaaa ctccgaccta
      961 ccagcactga cgatggagcg gacgctgcgg gtgcatttcc tgtagcggtg gttgagtcta
     1021 ggtgaccccg cggtcgatac gagagttcgc cgccatcgat cccggacggg aggcgattcc
     1081 cggcgagacg acggtgtgca ggttccgcct gccgcttgag cgccatcagc tcgggccgca
     1141 gatcctcgcg accgtgaatg cccacctcaa gcgcagcggc atcaagatca gcgccggcgc
     1201 gatcgtcgac gtgctggcct agccggtgca cgtgcgtatc gacgaactca acaagctcgt
     1261 cgacttctag aggtcgcgat tgcgatccaa cgtcgggcac tggatcgggg tgatcaagcg
     1321 cctcctcggc ttctaagagg tacgccaccc cggcttggcg cagaccggca tcgactcgag
     1381 gttactgcgg cactcgccga ccggttcctg gtgcggcgac aactactgcg atcgcaggag
     1441 tgatgtcgat atacgttggc gacagtgttc acaaatagtt gacgtttcag ctgaaacgtt
     1501 acgcggcggc caaattcgcc tgccgcacgc cttcgacccg gaatccgtta ctctcatcga
     1561 cggtcattca gacattgcct tacgtgtcgg acggtggcct accctgtgac ccgcgccgac
     1621 actcctcgta acggattcca agacagtcga gaccctcttt gatcttgcag aaaatcgcct
     1681 cgcgttcctc gtcacttatg gtcgccaggg cgccgccagc ctttcggatc gtaagcgaat
     1741 ctgggtagat tccggtgaca agagcaaaat cggcatccaa aaccgctgtg atttcaccgc
     1801 gcgcgtactc gattcgatct cgactgccga cctgcacaat tgcgccatcg gtgtctctcg
     1861 cccgctgcgg tgcgatgcga gtgaacatca gtggcctcct gtaatgacgt tcactatttc
     1921 tggatttcga agaagtgctg ggtattcaac cgtactgaag atgctactga ccctcgtgcc
     1981 actgacaaag ccaaatacgc tgccggacgc tccgtcggca aatctcgtag acacgcgcga
     2041 ccaaacctga acggcctgtt cagcggaaat cgggctgttg gggccgaaaa gctgttgctg
     2101 gtccaaccaa ttgccgccag gcgtcatttc aagcgtgctc cttccattgg cggtagcaaa
     2161 ctcttcagct agcgcgcgat tcccgggccc ggaatagaat acagccccat tcctcgcggt
     2221 ccggatatca agcgtttcgg ctcgccgcat gagtcggttg aaggcggcct tgctccctga
     2281 cacctgtcgt gcccgagcaa ggttccccgc gacgcgcgcc agaggtatgg cgccagcagc
     2341 caggccttcg cataccttcg atacgccgca gattgccagt ccggcatcca ggactgcggt
     2401 gccgtaccgc cggtccctcg ccgagtcata tgcgtcgatg gcgtcgtaca tcggcgccag
     2461 gatcaggacg tcgaccgcat tgttcccgag atcaatgaac aactgctcga gaatctcgcc
     2521 tgcgtaagga tcgtcggcgc cagtctcgtt gacccagctc gacgtcgggg cgccgctccg
     2581 cagaatcggc gatcctgccg ccgacagcac ccacccgccg cacgcgaccg tggactgccc
     2641 cggcccgcac atgactccgc cggtctgcgc cgactggccc ggcattgcac tggcgggtgg
     2701 cacgtaacgg tcgcctccgc cgaacaacag gtcgccgacc gtcttgatga cggagcccac
     2761 caccgagacc gtcaggacgc cgccggtggg atcgagtccg gacggatcgg tgagcaccag
     2821 cggccggttg cccgcgtagg cgtacggatt gaccgtctgg ctgttcccga gatcgccgat
     2881 gaccggatcc accgacagga atcgtcccgt ccccgggtcg tagacacgcc cgttcatgtg
     2941 gatcagcgcg acgttgtcga ggtgctcgtg gccggtgtag ccgtggcgcg tgctgttggc
     3001 gatgcccgcc cagtcgggcg tcgtgccgga actccagtcg cccccgcgcc gcccgccgaa
     3061 ggcgtcgaag ctcgtgcgcg ccgtgacgct gccgctggca tcgagcacgg tatccgtgcc
     3121 gcccaggtgg tcggcgagcg cgtaactcgt ggaaccggtg ccgtccgagt ttcgcgacac
     3181 gacgatcgcc acgccggaag gcgtgtacgc gtaatggcgc cagtaggtcc ggcccgtcga
     3241 cgtcgtgctc tccttctcca gcagcccgcc gacgtagtac gtcgtctcgg tgccattcgc
     3301 gtacgaggcg acctgcttcc agcgccggtg gtccggcccg tagctgaact ggctctggta
     3361 agtgacaccg tcgatcggct gcgcgagttg aaccggcagg ttgaacgagg cccaggcctg
     3421 cgatgcgccg tcgcgggtga cctggttgcc gttggcgtca taggcgtagg tgtggctgcc
     3481 ggccgcggtc acggcgtgcg gatgtgcggc gctgccgtag gtgtaattgc cgacgtccga
     3541 ccgactggtg acgttgccgg atgcgtcgta ggcggcagcg aaatttgcga cgccgttgag
     3601 cgtggatgac tgcacgcgat ccagcgcatc gacggtgaat gtctcggtga ggccctggtt
     3661 caggtcctgc cgctgcgtga ggttcccggc cgtgtcccac tggtaggcga ggttctgccg
     3721 attgccggtt gccggcagga ctccggactg gcggctcact atttcgccgg tccatgcctt
     3781 gtatccggag gccaccctga gcaggcccgc cccgagggtt tcgagggtcg ggagcgagtc
     3841 gtcgttcgcc tggttcagtg tccacagcgt gcgggtggac gcgggtgcgt cggtgacgtc
     3901 ggtgacctgc accggctcgc catagctgta cgcgtaccgg atcttgaacc tggcacccgc
     3961 ctgcccggtc ggcgtcggac tcgacgggta cgtgagtgtg tccagcgcgc ccgccgagtt
     4021 gtacgcgaag tcgtactggt aggactggtc ggtgacgatc gtgcgactcg ccaccctgcc
     4081 cgcaccgtcg tacgcaagat tctccgcata accatagcca gcggccgact gcagacgccc
     4141 gatgttgtgg gcggcagccg acgctcccca ggtccacgtg ctcgtgccgt ccggctccgt
     4201 gcgggcgacc gggcgaccga gtgcatcgta ggcgaccgag aacgactgcc cgcgcgcatc
     4261 ggtccacccg accagttcgt tcagcgagtc ggcggcgaac gtccacgtgc cgcggtccgc
     4321 atcggcccac tgcgtgcgga atccgcgcag gttgtaccaa ccggccgaga ccgcaccgat
     4381 cggatcctgc acgcgattca ggtttccgaa ggcgtcgtag tcatagctgg tcgtgccgcc
     4441 tggcggcgga tcgacgacgc gtcgcagcct tccctcgacg tccacgaccc gcgtgcgcgt
     4501 gcgccccagc gcgtccgtcg cgcgcatcgt ccggcctgca tatgcgaatg agctcgctcg
     4561 atcgaccgct ccgttcggct ggtagagctt ccgtgcggtg acgcggtcga gcacgtcgta
     4621 cgtccagccg acgtagccgt tggccgggcc agacgtcggc cggtagcgcg tgaccggacg
     4681 cccgagactg tcgtacttcc aggtctccgc gacccaggcg ccgagcgcgc gcatgtactg
     4741 gctcgagcgc gggcgctcga agccgtcgaa gagctgctcg cgctggtcgt agacttcgcc
     4801 ggccgagccc ctggacgtct cgtagacgtg aaacctgagg tccccctggc cccagcatgg
     4861 cccggtgctg caggactcga aggcccatga cgtggtggtc tggtcgggcc gcgtctcgag
     4921 cacacgccgc ccgaagtcgt cgtactgcca ggtcgtcgtg acgccattcg gatccgtcac
     4981 cgagaccgga acgccgaagt cgtactggta gctcacgcgc gtggcctgac ccagcgcgtt
     5041 ggtcagcgtc tccggcagct ggcagcgagg cccgtagtcg aatctcgtgg tgcgcgcggg
     5101 cattgcggcc ccagtcgagg ccgagcccac gacctgcacc gaactcacgt tgccgcagcc
     5161 gtcgaacgcc agggtcgtcg tgaccttgag cgacggcgtg gccggctcca ggacctgttg
     5221 ccagatccgg cacgcgcccg tgtccacggt gtatgcgacc gtgcgtgcct gagacggctg
     5281 cccgggtatc gtgcgtgtga cgacggacga caccggcagg cccaggcagt tgctcgtagc
     5341 gtcgttggcg tacgtggtga cggtaatcga ctgccagctc gaccctgaga acggcgaagc
     5401 cgggtccttg tccgtgacga cacgctggac ctgcgtgcga ttgccgtagc cgtcgccgta
     5461 cgcatacgtc tccgtcgcct gcgtgaccgc ctgaccgtcg agagcgccgc ctgcctcgta
     5521 tcgtgtctcg accgcactcg cgaggtgcgg gaacgcgcgc tgctcaaatc cggcgcccag
     5581 cgactgccag gcgggagtcg cactccattc gctcaccttc gacgcaaggt tcgattgcag
     5641 gacgacgcgc cgcgtgtgca ggcccgtgta gggaaacgcg cgctcgaaat agtcgtacgt
     5701 gtacaggccg ctgcgggagt cgtagctgcg ccgcgaaccg aatccctcga acccgcgtcc
     5761 ctgcaggtgc acgcgcgcgc caaagtactg gaagagattc tgataggttc cgccggtgcc
     5821 atccgacgcg gcgacctggc tcaccacgta cagcggaccg gcgtagtcga cctccgggaa
     5881 ggctgcgtcg gcgtgcttcg tgtagttgcc gctcgcgatg gagacgtacg acgcgccctg
     5941 cgacatcccg aagccgtcgc tgaacgatgt cgcgaggtcc gcgggcacac ccggcgcatt
     6001 gtgcaggttg tacttgatga ctccgccgcg ggcatcgtcg cgccaggcga ggtccgtctt
     6061 gccgtctccg tccgcgtcga cgacgaacca cgccgtcttg tccggggcag gcatgccgat
     6121 ggccaccggc gcggctgcgc cgatgccggt cgagcgcacc acataccagg tattgctgaa
     6181 ccacgagctg gtggcggcct tgacgtacag gaggtcggtg cggccgtctc catcgacgtc
     6241 cgcgggcagc acggtcgcat tgccgttcgc atcccgggcc atgccgaccg cgccggtcgg
     6301 gatttccgtg aactcgccgt tgcagttcga gaccaggacg ctcgatacct gcaggaagtc
     6361 cgagcagccg tcgccgttcc agtcgacgat cacgatgcgg ccgtccggcc agaagtcgcg
     6421 ttgcgctccc tccgtgaacg gcgcgtcgaa gccgttggac agcagcggcg tcagccgtga
     6481 cgcgccgccc ttcacggtgg caaccatgtg caccgccagg tcggcacgac catcgccgtt
     6541 gatatcggcg acgttgttgt agctgcgcgg gctgacgtag ccgccgctca cggtccaaag
     6601 cgtcacgcga ctggcagcga atgccaccga cgcgcccacg cccggcacgg tgacgttgcg
     6661 gcgaccctgc agcgtggccg gcgaggaaga cacgtgcgac aagaggtcat cgaggccatc
     6721 accgtcgacg tcggcggcga gatactcgcc gtccgggacg atcgcgacgg gtgtcagccg
     6781 gatcgccgtg ccttcgcagg cgacgagcac ccacgatccg ctctgctcga cgaggacctg
     6841 cgtgcggccg ttgccgagga accgtcccgt gatcacgtgc gccgcgacgg tggacgtgac
     6901 cccggtgtcg acgggacttg cgtagccgcc gggccgcgcc aatgcgatcc accagccgag
     6961 gctgccgctg ctgaccgcac gcggaagcac caggtccgac aagccgtcgc cgttcaggtc
     7021 caccgcacgg gtggcagcgc tggcactcgc aaccgctccc gtgctggcgg ccgtcgacgc
     7081 ccagcctgtc gcgccctgct ggtacgtgac gacggtcggc gcgaggcaac tcgtcggcga
     7141 acactcctgc acgctgcgca gctgcaggcg accggtcgcc ggtccctggg cgtaggacag
     7201 cctgtaggac ttgatcgtcg ccccgctcga gacgctggcg accgtgatcg tgtcgagctg
     7261 gtttggctcc ctgaccacgc tgccggccag gtatcctgcg acgatgtcgc tcgcgggtcg
     7321 cgcggagtac gcgaacgaca cgcgatagaa ggggccctgg ccggtggccg tcaccgggta
     7381 ggtgatcgac gaaacgcgaa tcgtgccgtt ggtgtaggcg ccattgcgtg cgtcgttcgc
     7441 ataggagatc gcgatgctgt tgccgctggt cgtagcggcg cgatcgcgga tcctcgacag
     7501 cgcccaggtg cggaccgtgc cgctcgaacc ggcgtacacc tgcgagtcgg aggccgtacc
     7561 gaattcgtac acgagcccgt tcttgctggt gaccgtgaac gacgccgggc cattgccggc
     7621 gatgccgctg gcgacgatct tcgagaacga ctcgacttcc gtggcgtata cggaatcggg
     7681 cgcgccgtag ctgccgctca ccagcttcag ctgccggccg tcgaggcaat agcggtcggc
     7741 gagcgcgttg ccgaccgcgg ctggagcgcc gtcctgcgcg atcgttcgat tgcagcgcgt
     7801 gatgacggag agcccggaca gcgcccaacc ctgccccagg acgccgttgc cggcgcggct
     7861 gttgtacacg agcgcgaggt cgaggccgac gctgccgaca cccggcggag tccagatcgg
     7921 gatgcggtaa gtcgctgcgc cggagctggt gaccccgaag ctacccggcg tgcggccggc
     7981 ggccgtcgcc gccgcgaccg aagccaatgg ctgggtcggc acggggtcgg gcggcacgat
     8041 cggctctggc tcgaccccga tgacgtcgga ggacggcaga tccgtcggtg cctcgaccac
     8101 gaatccatcg ggcgacggcg actgggtttc gatcgcccag ccggcgccgg cgagcagcgt
     8161 cagtgcgcac gcggcggcag cggtggccag gatgcggatg gaatcggccg atcgcgaccc
     8221 tgggctcgcg tcgattcggg tatcgcgagc cagacgctgg actgcacgag cgttgcgcat
     8281 gtgacctcct tgtccgccgc gggcgtcgcg actgcgacca cccgcctgtg aacggcgact
     8341 tcctgtcgcc ggtcgctcgc cggggcgggc atcctgccca ttctcggaaa gcacgcgcag
     8401 gctacgggcc ggcgaccgct cggtcactcg tgatttctgg ccggaacgtg cagttatgcc
     8461 gcggcgcgcg cccggcctgc gatcacgccg gatggatgaa cgcgtctgcg cgcgtccggc
     8521 cggccgccgt cagaattcct tctgcaccag gaaatagaac gtcgcgtccg gcgacgcctc
     8581 cacctggatg tcctcgttca tgccgaggtc gaagcgccag ccgtgctcgg tgcgccacgt
     8641 gccgccgtag ctcagcacga tcgcatcgcc ggacagcccg ttctgcggca cgtcgaagac
     8701 cttggagttc gcgtagaact gcacggtgag atcgagcctg cgccacgcgt tccacgtcac
     8761 gcccgcaaga cccgcgacga cgaaattctc ctgctcgccc ggcaggatgt cgccgttgcc
     8821 gagatacacg gcatccacct gcccgaacag ctgccatttc tctgcgagct gcgtctggcc
     8881 cgcgacgctc aacgcgacgt cggtcccgcc gctgccgagc aggtagtcgc tgtcgcctgt
     8941 cggcaggtcc accgtcagcc acgccgacag cgcggacttc tcgttcgaca tcagctggta
     9001 gcccgtacgg atggggatgt ccgcgatccc cgacccgctg tcgtcgacgt gcagcagcac
     9061 ttcgccgtca cgctggtagt cgatgagcag ccggtccttc ggcatgttct tgcgcggccc
     9121 ttgcggcagg ttccagaagt cgtgccagtt gtcgatgaaa ccatcgaggc tgccgccgct
     9181 caggtcgcgc cacggcagct ccaccatcag cgaccagtcc tgccacggcg agtattccag
     9241 gcgcaggcgg acgtcgacgg tctcggcgtc gagggtgaag tcgtcctggc cggacgagtc
     9301 caggctggcc gagttcgacc aattcacgtc gagggctgca cgcccgtggc cggcgtcggt
     9361 cagtcgggct ggcagcggca tcggcagccc gtacggcagg gtcagcggat tctggttgcg
     9421 cgtgatgagt ggctcggcct gcgacgactg cgccgcggcg ccagcgagtg caagcgtggc
     9481 ggcggcaagg actgcgccgc gaatgcagga tccgcacggg cggatgcgag gcgtgctgtt
     9541 cattggattc cccgcaatga cggcctcgga gtctaggcga cttccgccgg ccggccatcg
     9601 tggaatcttc agatggggac attcaccatt ttccggccgg aaaatggtga atgtccccaa
     9661 ttcaggccag gcgcagcact ttcgcgccgc gcacccgctg cgtcttgagg tcgagcaacg
     9721 cccggccggc atcacgcagg tcgtaggtct cgacctccgg gcgcaacggg atctcggccg
     9781 cgagcgccag gaactcccgc acgtcgacgc gcgccacgtt cgcgacgctc cggacctcct
     9841 tctcgagcca gagatgccgc gaatagtcga gatccagcag gtgccccttg tccgcgtctt
     9901 ccttgcggat cgcattgacg accagccgac cacccggctc gagctgctcg agcgcgccga
     9961 gcaccggcaa ccatgcgggc gtcgtgtcga tgatcgcgtg cagcttcacg ggagcccggt
    10021 ccgtcgtgtt gcccgcccag gtcgcgccga gttccagcgc gaatgcgcgc tcctccgggt
    10081 gccgcgcgaa gacatatacg cgcgccgatg ggtatcgatg tctcaccatc ttcagcacca
    10141 ggtggccgga gccgccgaag ccggttagcc cgaggcgcat tccgtcgcgc acaccggcca
    10201 gtcgcagcga gcgatagccg atcgcgcccg cgcacagcaa tggcgcggcg tcgatgtcgg
    10261 agatcgcgtc gggaatcggg tgcacgaact cggccggcgc ggtcatgtac tcggcgtagc
    10321 cgccgttgac gtcacgccca gtcgcacgaa accccggaca gaggttctcg ttacccgcgg
    10381 tgcactgctc gcagtgccca cacgccgaga agatccaggc gacgccgaca cgatcctcga
    10441 tcctcggccg cgcagaaatt ggcaagtcgg tgcctggcac cgaatcgccg tattcaatga
    10501 tgcggccgac ggcctggtgc ccgggaatca cggggagtcg cggcggcgca gttcggccct
    10561 cgatctcgtc gagttccgtg tgacacacgc cgcaggcggc gaggcgcagc aggacttcgc
    10621 ccggcgcggg ctcgggcacg ggcacgtcgg cgaactcgag cagggctcgg cccgccacga
    10681 ccggccccaa ttcgcgcagc agcatcgctt tcatggtcgt gatggctcga cgggacgggt
    10741 aaatgtcgcg gctcagtaaa tcaccaccga ccggatgctc tcccctctgt gcatcaggtc
    10801 gaatgcgtcg ttgatgcgct cgagcggcat ggtgtgcgtg atcatcgggt cgatctggat
    10861 gcgcccttcc atgtaccagt ccacgatgcg cggggtgtcg gtgcgtccgc gcgcgccgcc
    10921 gaaggccgaa cccttccaca cgcggccggt gacgagctgg aacggacgcg tcgagatttc
    10981 cttgcccgcc tctgccacgc cgatgatgac gctcgtgccc cagccacgat gacaggcctc
    11041 gagcgcctgg cgcatgacgg cgacgttgcc cgtgcagtcg aacgtgtagt ccgcgccgcc
    11101 gtcggtgagg gtgacgaggt gctgcacgag gtcgccctcg acccgcttcg ggttgacgaa
    11161 gtgcgtgatg ccgaagcgcc gtccccacga ttccttggcg tcgttcacgt ccacgccgac
    11221 gatcttcgcg gcgccgacca tcctgaggcc ctgcacgacg ttgagcccga tgccaccgag
    11281 cccgaacacc acgcagttgg cgccggcctc gaccttcgcg gtgttgacga ccgcgcccac
    11341 gccggtggtg acgccacagc ccacgtagca cgccttgtcg aacggcgcgt cgtcgcggat
    11401 cttcgcaacc gcgatctcgg gcaggaccgt gtagttcgag aacgtcgagc agcccatgta
    11461 gtggaagatg ggccgaccct tcaatgaaaa gcgagtcgtc ccgtccggca tcacgccctt
    11521 gccctgcgtc gcgcggatgg cggtgcagag attggtcttg cgcgacaggc acgacttgca
    11581 ctgccggcac tcgggtgtgt agagcgggat gacgtggtcg cccggtttca cgctcgtgac
    11641 tcccgggcca atctcgacca cgacgcccgc gccctcgtga cccaggaccg acgggaagat
    11701 gccttcggaa tcgaaaccgt cgagcgtgta ggcgtcggtg tggcagatgc cggtggcctt
    11761 gatctcgatc agcacctcgc cggacttcgg tccgtcgagg tccagctcga cgatctcgag
    11821 cggcctcctg gcttcgaatg cgacggcggc ccgggtcttc atcggtgatc actcccgcgg
    11881 caaaggttgc cggtcacggc gcagctatcg tgccgcgtgc gccttcgtgt actgtgcggc
    11941 aaacgagcgt gaaaacgcca gtccccgggg aactgcgatg gcggacttgg tcgagtgcgg
    12001 cgcttgcgac atcgtgaacc gttgagccag gcgtcgccat gaacggcgaa acgcaggtct
    12061 cacgtacgac ggtcgacgag tgggcctggg atcctgcgac gaacccgctg ccggcgtccg
    12121 tggacgtggc gatcatcggg ggcgggatcg tcggctgctg cgcggcctgg ttcctggcca
    12181 gagaaggcct gtcggttggc ctgttcgaga agggccgtat cgcaggcgag cagtcgggcc
    12241 gtaactgggg ctgggtgcgg cagcagggac gctcgcccgt cgaactgccg atgatgatgc
    12301 gcagccggcg gctgtggccg gagctgcagc gcgaactcgg cgaggacctt ggattccggc
    12361 agggcggctg catgtacctg gccgcgaccc aagtgcagtt ggacgtgatc agcccgtggc
    12421 tggtggtcgc tcgcgagcat ggcctcgaca cgcgtttgct cacccgttcc gagctcggcg
    12481 gagttctgcg cggcgatctc accaagtggg cgggagcgct ctacacggcc agcgacggac
    12541 gcgccgagcc gaatcgcgcc gccccggcca ttgcgcgcgg tgcgcggcgt gcgggtgcac
    12601 acatcatcac cggatgcgcg gtgcgcggat tgcagaccgc ggcgggccgg gtgcaaggca
    12661 tcgtcacgga gaaaggcgcc gtctccgcgc ggtccgtcgt ctgcgccgcc ggtgcctgga
    12721 ctcgcctctt ctgtcgctcg ctcggcatca ccgtgcccca gctcatggtc ctcggcacgg
    12781 tcgcacgagc ggcgccgagc gacgccgtcc tcgaaggcga agtctgggca ccgaccctgg
    12841 ggatccgtcg ccgcgcggac ggcggccaca cgatcgctca cggcgcatcg ttcctgcacc
    12901 gcctgacgcc cgacaccttg cggttcgcca cgaaattcac cgggctctat cggggcactc
    12961 actcgatgcg cgtccgggcc ggcgctgagt tcttccgtga cctggccatg cccgcccgct
    13021 gggacctgga tgcgccgagc ccgttcgaac gccaacggat gctcgatccg gcccctgaga
    13081 cgggggttct acgccagatc cgcggtgaac tccggcagtg gttcccggag atcgccgacg
    13141 tgcccttcgt cgagacctgg gccggcatga tcgaagcgtc gccggacgcg ttgccgatca
    13201 tctgcgagtc cgatcgcctg ccgggcttcc acatcgccac tggcttcagc ggacatggct
    13261 tcggcatcgg acccggcgcc ggacgtttcg tcgccgacct cgtgcaaggc aaggcggatc
    13321 cgaccgagct cgcggcgttc cggctcaatc gctatttcga cgggacgccg atccgccccg
    13381 gcccatcgct ttgatcgcgc gcctcgcccg ggacgattga ccgcgagtcg gtgctgcatg
    13441 caatgtgagg catggcgggt agctcgctac gccagttctg ggcggagtta cggcggcggc
    13501 acgtgccgcg cgtcgccgcg tactacgtcg ccggcgcgtg ggttgcggcg caggcggcca
    13561 gcctgctgct cgacgccttc gacctgggtc actacacgcg ctacgtgatc gtggcgctgg
    13621 gagccgcctt cccggtcgcg ctggtcctgg catggcgctt cgacatcacc cctcgcggca
    13681 tcgagcgcac gctggaccgc gcagtccccg ccgctcctgc gtcgcaggcc ccggcgcccg
    13741 agcgatcgat cgccgtgctg ccgctcgcca atctcagcga cgaccctgcc aacgagtact
    13801 tctccgacgg actctccgag gagatccgca accagctcgc gcgcgtcccc ggactgcagg
    13861 tcgcggcgcg gcgctcgtcg ttcgcattca aggccagcgc cgtggacgtg cgcgagatcg
    13921 ggcggcgcct caacgtcgcg acgctgctcg agggtggcgt gcgcaagcac gccggcaccg
    13981 tccgcatcga cgtgcagctc gtgagcaccc gcgacggctt tcatctgtgg tcgcaggggt
    14041 tcgagcggcg gctcgaggac gtcttccggc tgcagaccga ggtcgccgcc gcggtgctgg
    14101 aggcagtgcg ggcgcgccac ggcctcgagg agtgcacact gccggctccc gacgagcggc
    14161 gcttcgaggt ctacaacgcc tacctcctcg gccgtcacca cttccacagg cgcagtgagc
    14221 agtcgctgca gaaggccgcc cagtgtttcg agcaggcgat cgcgctcgac ccgggctacg
    14281 cgctcgccta cagcggactc gcggacacgt acctgctgct gggagggcgt tactacgggt
    14341 cggcgaccac cgccgactct gtcgcccgcg ccgagccctg cgcgcgcaag gcactcgagc
    14401 tggccccggc gctggccgaa gcccacgcgt cgctgggact ggtgcagttg aaccgcggcg
    14461 aggcggcgct cgccgcaagc tcattggcgc gtgcgctcga gctcaatccc ggctacacga
    14521 tggcccacgt ctggtacggc ctcgcgctca ccgcgcaggg tcgctaccgg gatgcggcca
    14581 ccagcaatct cgaggcattc cgactcgacc cgctgtcgcc catcgtgaac accaacgtcg
    14641 gcttcgacgc cctgcgattc ggcgactatg ccgaggcaga ggcccgcttc cgcacggcga
    14701 tcgaggtgga cccgggattt gccgtcccct actcgggcct gtcgaaaatg gcgatggacc
    14761 gcggtgacac cggcacggct catcggtgga tcgacgctgc catcgagcac gccccgacgc
    14821 gggccttcta ccgctcgcgc aaggccctgc tgtcgctgca gtcgggcgac ctcgacgctg
    14881 ctgccgagca cgccgccgag gcgaatcgcc tcgcgccgca cgacgtgctg aactgcgact
    14941 tccaggtcgc gctgcacatg gcgcgcgcgg accgcgacgc gatcgcccgc atcgcgtccg
    15001 gcggctgcgg accggactac tccacgggtg ccgtggcaca ggcgtgcatc gcgctcggcc
    15061 agctggatgt ggcgcgtgaa ctctacgagc acgacgtgcc ggacatcgac cgcatgatcc
    15121 acgagatcct gaacgacgac tggacctggc gcctgccgca tcccatcaac catgcgcacc
    15181 tgttgttgcc gctggacgcc gctcgtgcag gagccgggct gaatgccgtg ctcgccgcat
    15241 gtgaccgcgt cttcgcggac ggcatcgtga acggcagcct cacttatctc gcggcgacgg
    15301 cgctcgccgt actgggacgc agcgaagcgg cgcaaggcaa gctcgccgag gcaaggcgcc
    15361 tcggctggag ccacgactgg tggcgacggg tcgactggaa cgcgcgcgga ctggattgac
    15421 ccggccagcg gcgtgtggaa tgcgcgacgc cctgatcagc tcgccatgat gaccggcccg
    15481 cccttctgca gtgcgcgctg gtaagcgggc cgggcgttga tgcgcgaccg gtattcgacg
    15541 aggtgcggca ggcgcgccgc gtatgacgcg cgcgacagca gcgcctccac ggcgaagctc
    15601 atctggaaat cggcgactgt cagcaacgga ccggcgaacc agcgattgcg gccgaggtgc
    15661 ccgtcgacgt aggaagccgc cgtcgcgagg ttcgggtcga tcagctgccc ctgcaccttg
    15721 ttgcagatgg cccgggcgat cggccgcgcg aagaacggca tgggctgctt cgggagcgag
    15781 ccgaacacca gcttcatcac cagccagttc atcagcgagc cttcggcgta gtgcatccag
    15841 tagcggcact gccagtcctc ggccgagccg cgctccggga cgagaccggc gagctcacct
    15901 ccggcgaagg acccgtacgt ttcggcaatg tattcgatga tcaccgcgga ttcggcaatg
    15961 accttgccgt cgtccgtgat gaccggtgac ttgcccagcg ggtgcacctt cctcagttcc
    16021 ggtggcgcga gccgctgggc ggtccgcttg tagaccttga gttcgtatgg cgtgccgagt
    16081 tcctcgagca gccacagcac gcgctgcgag cgcgaggtct cgagatggtg caccgtcagc
    16141 atgcaatcgc tcccctgttc gccatcgtct caccaggcga cggcgcccta tccgtgtccg
    16201 accgatggcc gcgtcagcgg gcgcggcggg tcttcgcctt cttcgcgcgc gcggcggtct
    16261 tgcgcgtcgc ctttcgcgcc ttggccttgg ccttcgcctt gcgcgctgcc ggcttccggg
    16321 cggccggagc tgccttggcg gcccgggcgc ctacctcgcg cgcctgctcg gccgcctcgc
    16381 gcaggatctt catgccgcgc gacacgtcgt tcacgatgcg ttcgcgcgcg gactgcagca
    16441 cccgcgcctg gtcgcgcccg aaatcgcgcg cgctctccgt gtaggccagc ttctcgatct
    16501 gcgtcgccgc ttccgcaagc gccgccgtga cgatctcctc gtggagcccg atcaaccgct
    16561 cgagcgtccc gtgcgagatg ttggacagct tgacgccgga tcgcgccatc tcgcgagccc
    16621 gggcgttcag cgaccggacg cgggccgcag acctcagggc ggccttgcgg gccgtctcga
    16681 gctgcgactt gcgcaggttt tctgccatct ccttgaggcc ccgggtctgc tcctccagaa
    16741 actcgttcat tcccgcgtgc atgactgttc cctcgcgtgg cggccctgac cgtttcgagg
    16801 ccgtccgtgc aacatacgcc gacggggacc gcggtcaatc cccaccgtct ccgggcactc
    16861 cggcgacgcc acccacgcac cgccggtgtg cggcagcctg ttccaaggac acctgccctt
    16921 gtcggccagt ccggccccgg gcaagattgt ccggacgacc ggtcgattcc ccccgcaagc
    16981 cgcagcagcg aggtccgtgc gagccccatg gcccacgacg tgttcctgag tcatgccgcc
    17041 gcagaccgcg cggccgcgct ggcggtcctc gagggcctcg aggcggccgg catccgctgc
    17101 tggatcgcac cgcgcgacat cccggcgggt tccgagtacg gccagcagat cgtggatgcg
    17161 gtcaagggct gccgcgcgtt cgtcgtgatc ttctcggcga acgcgaacgc ctcgccgcac
    17221 gttcggcgcg aggtcgagcg cgcggtgtcc accgaccgcc ccatcgtccc gttccgcatc
    17281 gagaacgtca ccccgacggg cgccatggaa tactgcctcg gcggcacgca ctggttcgac
    17341 gcgctcacca cgccgctgga gccgcacgtc gcgaacctca tcgcgacgct gaagcagatg
    17401 ctgggggcgg ctggccggac cgcggagggc aaggcgtccc aggaagccgc cgagcggccg
    17461 tcgatcgccg tattgccgtt caacaacatg agcggtgacg ccgagcagga gtacttcagc
    17521 gacgggatca ccgaggacat catcaccgac ctgtcgaagg tctcggggct gttcgtcgtc
    17581 gcgcgcaaca ccgccttcac ctacaagggc aagccggtca aggtgcagga ggtctgccgg
    17641 gaactcggcg tgaaatacgt gctcgagggc agcgtccgca aggccggctc gcgcgtgcgc
    17701 gtcaccggcc agctcatcaa cggcgaagac ggcggtcacg tctgggccga ccgctacgat
    17761 cgcgacctca ccgacatctt cgcgatccag gacgagatca cgcacgccat cgtggagcag
    17821 ctcaaagtga agctgctgcc gcaggaaaag cggtcgatcg cgcaggcgcc cacggacaac
    17881 atcgaggcgt acacctacta cctgcgcggc cggcagttcc tgcaccggca cacccggtcc
    17941 tactaccagt tcgcccggcg catgttcgtc aaggcggtgg aactcgaccc ggcctacgcc
    18001 cgggcctacg ccgggatcgc cgactgcgac tcgttcctgt acctgcacta cgacatcggg
    18061 atcgccatcg acgacatcct ggcgaccagc gcccgcgcgc tcgagctgga cccgggcctc
    18121 gcggaggcgc acgcgtcgcg cggcctggcg ctgtcgctgg cacagcgcta cgacgaggcg
    18181 atggcggaat tcgagcgcgc gatcgccctg gacccgaatt cgtacgaggc caactacttc
    18241 tacgcgcgcg cgtgcttcgc gcagggcaag ctgccgcagg ccgcgcagtt gctcgaacgc
    18301 tccgcggggc tcaagccgga cgactaccag gcaccggcga tgctggtgat ggtatatcgc
    18361 tccatcggcc gcgaggccga cgtgccgggt gcggcgcgca aagtcgtcga gctttcagaa
    18421 gccgagctgg gacggcaccc ggagaatccc cgacccgcat acctcggcgc gggcagcctg
    18481 atcgagatcg gcgagatcga ccgggccaag gagtggctct cacgggccgt cgcgaccgat
    18541 cccgacgacg tgctcacgca atacaacgtg gcctgcatct attccaagct cggcgagtac
    18601 gacgcggcct tcgacctgct cgagcggctt ctgccacagg ccaaccacga gacccggtcg
    18661 tgggtgcgcc acgacgcgga cttcgacccg ctgcgcacgc tgccnnnnnn nnnnnnnnnn
    18721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18781 nnnnnnnnnn nnnnnnnnnn nnnnagaccc ggtcgtgggt gcgccacgac gcggacttcg
    18841 acccgctgcg cacgctgccc cgttaccaga aggtcctgca gctgatccag tagtccgtgc
    18901 tggaaaggac ggctgacacc gagcgcctgg gcgcgccgcg caggccgatt tcacttgcgc
    18961 acgacgcctc ttgcggcggt atcacgggct gacttgaaca tcttgagtcg gtagtgagcc
    19021 tcgcgctcgc gttcgccgag gaactgcacg atcgcgcgga tgtcggcgcg caagcgcggc
    19081 aggagccgct cctcgagcac gcgcgtccgc cgcgtcacgc gcaggatctc ctcgccgagc
    19141 atcttgagct tgctctcgaa cgcggcgctg ccgagcatcg cctcgatgat ggactcgaac
    19201 aggtgagttg cctcgtcgag gtgcggcgtg gtgacggtgt agtcgtagcc gcgctcttcg
    19261 ggccggcgca cgaacgggcc gaacacggtc agctcccggt agcgtacgcc gagcgcgttg
    19321 cgctcctcga cgtccacccc gaccggtcgc tccgccagcg cagcgagcga ctcgatcccg
    19381 gccgtgccct cgtgaccctc gctgagtcgc agctcggcca gcgcgcgcag gtaatcgcgc
    19441 cggatcgcgt cgcgcgatcg caccactggc cggatcgact cgaggaaccc ccggatcagc
    19501 acctggcgtc gcgccctcag gattgcgacg ctgttggcca ccgacgcggc cttctcccgc
    19561 agctgacgca ggtcggttcg cgtcgggtgg atcacggcag gcccagtccg aagcgctcga
    19621 gcagcgctcg gctcaagtcg agagtctcga cgatcgtgcg ccgcgcctgt ccctgcgcga
    19681 ccacctcgcg ctcgaacgca tcggcgaagt cgagcatgcg cttgtccgcg tcacccagtc
    19741 cctcgcgccc gacgatggct tcgagcctgc gcaggtcccg cccgttggca tagtgcatgt
    19801 aaagagcatt ggccacgtca cggtggtcct cccgggtacg ccccttgccg atgcccctct
    19861 gcatcagccg cgacaggctc ggcagcacgt ccaccggcgg gaagatgccg cgctggtgca
    19921 gttcgcggct caggacgatc tgcccctcgg tgatgtagcc cgtgaggtcc gggatcgggt
    19981 gcgtgatgtc gtcctcgggc atcgtgacga ccggcagcat cgtgaccgag ccacggcagt
    20041 ccttgatccg gccggcgcgc tcgtagatcg aggcgaggtc cgagtacagg tagcccgggt
    20101 acccgcgcct tcccggcaat tcctcgcggg ccgtggacac ctcgcgcagc gcgtcgcagt
    20161 agtgagtcat gtcggtcacc acgaccagca cgtcgcgccc gtgctcgaag gccaggtact
    20221 cggcgaccgt gagcccgaag cgcggcgcga gcagccgctc gacgacggga tccttggcga
    20281 ggttgatgaa cgccacgaag tctccgcgca cctgctcgag cgtccggcgg tagaaggcgt
    20341 attcgtggta cgtgagcccc agcgcgacga acactaccgt ggagcgggcg cccgcctgcg
    20401 cgcccgcgcc gctggtctgc gcctgctgca ggatgcgcgc ggcgatctcg ccggcgggca
    20461 gacccgcgca ggagaagatc ggcagcttct ggcccttgac cagcgtgttc aggccgtcga
    20521 tgcaggagag ccccgtctcg atcatctcct gcggtcgctc gcgcgccgtc gggttgagcg
    20581 ggaatccggc gaccggcgcc cacttctccg gcaggaacat cggcaggtcg tcgatgggct
    20641 ggaacgaacc gccgaagctg cggttcaaca cggcgagcga caacggcgcg cgcttgacca
    20701 cgtcggtgaa cacgacgcgc gagtgatcga ggtcgagccc gcgactgtcg ccgatcacct
    20761 ggatcagcac ggtgtcgccg tcgatgcgga ggacctcgcc ctccgcctcg tggccgtccg
    20821 ggaacacgat cgtgacgact tcgcccatgc gcgactcgag cactgcgtcg acgaacacca
    20881 gcggtccctg gatgctctgc agcgtgccgt agctatgctg gctgagacgc atggggttcg
    20941 ccgccgtgcg ccgcggaggc acggtcgtcc ttcaggattt cctgcagtga ctcgcccttc
    21001 tcgagccgcg ccgccgcccg gtcgtagtcc tccagcagcg cgcgaatcga tgcatgggtc
    21061 tgctgcggtg tcgagaacgc atcgtcggtg taggcgttct gtgcgaggaa gcgctgtcgc
    21121 acggcctcgg tcgtccgcat cagcagccgg tcggcgtcct gcaggccctc ggcgccgacg
    21181 atctccttga cctcgcgcag gctctcctcg cgctgcagca gcactcgcgc ccgggaccgc
    21241 agcgcacccc agtccggcgc gacctgcttc tcgaggtgcg cgacgacctc ggcctcgtac
    21301 agcgagaagc tctggaacca gtggatcgcc ggaaagtggc gccggtgggc gagtgacgtg
    21361 tcgagcatga agaacgcgcc ggtgttcctc aggcacgcct gcgtcaccgg ctcggtgaag
    21421 tcgccgccgg gcggcgagat cgacagcagc acggtcagcg aaccgatcct gccgtcgagc
    21481 gtctccacga cgcccgcgcg agcgaagaac tccgcaagcc tggagctcag gtacgtcggg
    21541 tagccttcct cgcccggcat ctcctccagt gccgaggaga tctccctcag cgcctcggcc
    21601 cagcgcgaca ggctgtccgc cagcagcagc acgtgctgcc cctggtcgcg gaagtactcg
    21661 gccatggtca cggccgtgta gatggacgcc tcgcgtgcgg cgaccggcat gttcgacgta
    21721 ttgacgacga cgatcgtgcg ctccatcagc ggacgctttc gccaggggtc ctcgagcacg
    21781 gaaaagtcgt cgagcatctc ggccatctcg ttgccgcgct cgccgcaacc cacgtagacg
    21841 acgatgtcca cgtcggcgta cttcgcgatc gactgctcga gcagcgtctt gccggtgccg
    21901 aagcccccgg gaaagatggc actgccgccg cgcgccagcg ggaacaggaa gtcgatgcag
    21961 cgctgcccgg tgacgagcgg ctcgaaggtc gagagcttgc gccggtacgg gcgcggcaat
    22021 cgcaccggcc actcctcgac agcttgctcc tgaggaggct ccgtgggcat ccgcggggac
    22081 aggcccggcc cgtgcgccgc atctgccgcg cccggccgca ggaatggacc ctgggtgcgc
    22141 cgcatctcct cgagcggccg ctgcaacccg tcgaacatgt gcccgagcag gccgggccag
    22201 agccgcacgg tgagctggct gcccgtgccg acgaccggct cgccgatgcc gagcccacgc
    22261 gtgctctcgt agagctgcac gatcgcgcgg ccccgctcga gccgcaccac ctcgcccatc
    22321 agtgccgcgt gcccgaccgt cacgcgctcg tacagccgca ggcccggcaa ctcgaccgtg
    22381 accgtgggac ccgagatgcc gacgatgcgc ccggccatca gctcagcctc acctggtagc
    22441 cgatcgcgcg gcggatgagt tgcaggacgt agtcttcctc tgcacgctcg cccggcatcg
    22501 gcgccggcag cacgatcacg agacccggcc agcggcggtc catgtcgcgc agcgcatccc
    22561 gcgcggccgc gtccaccagc cgctcgtcga cgatcaccac ccccgtcagc ggctcgcgcg
    22621 cgaccgccgc aagtgccgca ggcaggccct cggtgctggt cgccgcctgc cgcacgccgg
    22681 cgagcgcgaa tcccgggttc gcatcctggg gcgtgatgac gatcacgtgc ttcacgcgat
    22741 cagctcctgg tcgagcaccg cggcatcgaa gccggccgcg tggaacagga tcgagcggtt
    22801 gcgcgcgtgg acgtagcagc gccacatgta ctccaggatc gggccgactg ccgcgctgtc
    22861 gcgcgcgatc gcgcggacgc gcgcggtgag gctcccgagc aggatcgtct cgagcgccgc
    22921 ctcgtcgatc gagaccgggg gcgccgcccg cccggcgatc tcccgcacca gggcatcgag
    22981 cgtcgccgta tccttgctcg aggacgccgc tcccagccgc gagcactcga cgctgccccc
    23041 cgccacgaaa tccgtcgcgt cgccgcacgc ccagcgcagg tgcttgtaga gcgtcaccag
    23101 gttgcgcacg tcgatgaacg cgacgaagaa ccttcgcacc tccgtcggga gctgcccggc
    23161 ggtgacgcgc tcgagttgat cccgcgcgag ccgctgctcg aaccccttga gtccagcctc
    23221 cgcatacgcg cgctcgagtg cggcggatac gcccgcctcg cccgtccatc ctgccgcgac
    23281 ctcagcgacc gacgctgcga tgtccggtcc cagcagcaac ccgcgacgca ccacatcgtc
    23341 cagctggctg tcggcgagca atcgctgcac ttcggcggac cgttcggccg cccgggccct
    23401 caggcagagc acgatggtct tgacctcgaa cagcaggaat acgccggcga ggctgtcccg
    23461 gagacggcgg ttcatctgcg cgcgcagcca gccgagttcg tgcaggaagc cttcccagat
    23521 cgcctcgtcc gaggcagcgt cgtcgcgacc cgttgcgcgc actcgcgccc acgcggcagc
    23581 gagcgtcgcg cggcgcgccc ggaccctcgc gagcaggtag tccgtcgggt agccttcgcc
    23641 tgcgatgtca cggagcggca gtgtcatccc gctgctccgg aagcagcccg ccgacgagct
    23701 gtggaagtat gtcgggccag gcggtcgcga ggcgcgtgtc cagcgtattg ctgatccgaa
    23761 cgcgcccggc atcgccttcg agcaccatgc ctcccgagat cgccggatcg ccggcaacct
    23821 cggcatgcgg gaagctcgtg tgcgccaggt cggcgtcgcc cgggttgacc gagacccggt
    23881 cccacgcgag gccgggcagt tcctgcgcga gcgctgtgaa caatggcggg ccgccttcct
    23941 cgcgcagccg cggcagttcg gccatggcga ggtgccggca gcgctcggcg agtgcgagcg
    24001 cggcgcgggc gcgtctctcg cgagccaccc tgcgtgcgtc gaccaccgct tcgctcgcga
    24061 gcgcctgctc gagcccggcg gcggcctgag acgcggactc acgctcagcc tcggcgactc
    24121 gtgcgagttc cacacgatgc cgctcggctg cggcgcgcgc gtcggcccag acggcctcgc
    24181 ggtcctccgc cgccttggca cgcagcgact cgagcaaggc ctcgcggccc atcgctcagc
    24241 cctgcagcag gatcatcgcg gcgatcacga agcccaggat gacgatggtc tccgggatcg
    24301 cgagcaggat gatgatcgtg gccgtcagct cgggcttctc cgccagcgcg ccggcaccgg
    24361 cggcgccgat gcgcgactgc acccacgccg tcgacagcgc gctcacgccg agcgcgagcg
    24421 ccgcggcgaa cgccatcagt atcttgtccc agtgcattgc tgcatccatg acaggtctcc
    24481 tcagtcggct ttcctgagcg gctggtagcg ccggccgccg gtctcgatga acttgctgaa
    24541 gaactcgacg tagtgcaggc gcagcgcatg cacggtcggg gcgaacacgc cgagcacgat
    24601 gttgaacgcg tgcagcagga cggccaccgc gatgcccacc cagaggctgc ccgccgcgcc
    24661 ggccaggctg ttcgcgacgt aggcgagcag caccgaggcg aggccgacgg ccatgatgcg
    24721 cgcgtaggag acgatgttcc cgagcgtgcg caacaactcg aatggcgcga ggacgccgcc
    24781 ggtcagcagg accacgggcg cggcgatgag gatcgcgacc agcatcggct tcctgagcag
    24841 gcccgcgacc ggcgccatgt aggatgcgag caggccggcg accagcagca gcatgaggat
    24901 gctgatcccg cggaacaccg cttccttcgg gtgacgtccc cggatcgcgg cgatgactcc
    24961 cagcacgaga cccacgagca cgtgcacccc gccgacggcg agtgcgaaat agaacatggg
    25021 aatgatcgag gtgcggcggt cgatgaacgg gtggatgccg agcaggcgca cgccgaggtc
    25081 gccgaagcac tccccgtaca gcgcgccgaa gatgacggtg tagatcgagg cggccagcag
    25141 gatctggccg atcggtcgca gtgccggccg cttgcgtccc attgcgatca gcaccgccgc
    25201 tgcgatcgtg agcagcacgc cgtatccgac gtcgcccagg atcatgccga agaacagcgg
    25261 aaagaagatc ccgatgaaag gcgtcgggtc gatggaggtg tagcgcgggg tgggcagcag
    25321 ccgcaccagc agttcgaacg gacggaagta cggggggttg cggaacgcga ccgggacatc
    25381 gtcgaggtcc ttcttcaaca tctcgcgctc ctcgaccacg accgtgtcgc cgtgcctgtc
    25441 ggccagcgtg cggcgcaggt cgtcgaggcc ggccgagggc atccacccgt agatcacgaa
    25501 gcagcggtcc gtctcgtaga ccgaggcgct ggtcctgagc agcgcgaggc ggcgctgcag
    25561 ccaggagcgc atgctgaggt agtggccgcg ccacgcggtc gcaaattccc cttcctcccg
    25621 gtcgagggag tcgatgccgg ccgagagttc atcgcgcttc cgctgcagcg ctttcagctt
    25681 gtccggcagc gcgagtccct cgaggtacgc gggcaacgcg atctccggga tgtggttctc
    25741 gcgcagcccc tccttgagct tcgcggcgag atccttctcg gtcgtgagca gtccgatgta
    25801 ggaaccgtcc ttcgcctgcg cggtgcgcac ctccgcgccg agcagcacgc gtcccgccac
    25861 gcgggtcagc tgctcgaggg ccgcgggatc gcggacttcc acgccgatga aatcgaggcc
    25921 ggcggacgcc gcgcccttcg gcgccagcga atcgacggtc gtcaggaaca tgaggtagcg
    25981 attgacctgg gccagctcgc cctgcagcga ctcgcggcgc ctgcggcgct cctctgcggc
    26041 gtcgaggtgc ttgccgacga cgcttgcgat cgaactcacc gcgcgcgacg gactcaggta
    26101 ggactcgcgc gtatccacct tcggcagcag cgcaagcaac cgctcgacct tgagattcag
    26161 gtcctcgagg aacagtcgct ccgccagcgt cttgccgtcc agcgccagcg gccgcacctg
    26221 ggaaacgggg tcagacccca gatcgatgcg gtggcggatg tcggggtcga tctgcaggac
    26281 ctccgacttc tggatcgtct cgagcaccgg cagcagctgg ccacgcggcc cggtgatctc
    26341 gaccttggac atcctaacga gcacgggacg tccccggcac gattacggcg atgcactcgc
    26401 gcaccgcgcg cctgagctct tcgtccgacc tgcgatccac cgccagagcg agcgcctctg
    26461 cttcgcgagc gtgtcggtcc gcccgctcgg ctgccgcgcg ccgcacagcc tcgcgccggc
    26521 gctccgcctc ctgtcgcacg ctctccagct ccttgcgatg cgccgcgtcg atctcgcgcc
    26581 gcgcgtcctc gagccactgg ctcgcccggg tgcgctcggc gtcgagttgc tcgcggatct
    26641 cccgctcgac gccaagaatg tcctgaagag tgctgtgctc catgcgcagg gagccctgcg
    26701 gatattgccg ccatgctcct cggggccgtc gcgcgacggt cccgcgccaa aaagtctagt
    26761 ccgtactcac acttacgcaa tccggaatag aggaaccggc atgggtgaac ctgcgtgcag
    26821 tcggcagtga tcaggtgacc tcgccacaac ctgttcgcgc gtcacgggtg cagggagtcg
    26881 ccttgccacg cccaccgcgc cagcgtacct tcggcgtcac ttcgtccccc aggtgactcc
    26941 atgcccaagt tccgcccgtc cggccgtgtg ttgaccgtgg ctgccgccgc tgccctgctg
    27001 ctcgcgtcga acgcgtacgc cgatgtcgtc accggcacgg tcgcgcccca gggcgccagc
    27061 gtcgtcatcg tcgattccgc cggcaagacg gttgcgaagc tcccggccgg accgtaccag
    27121 ctgcaactgc cggtcggaaa atacatcgcc cggtgccagg caccgaaaca gcgcgaccag
    27181 gaattcctga gcctgtcgga tcccgtgacc gtcgacatca actgcggttg accgtcgagc
    27241 cgtgaccgac accgcggccc ccaggccccc ggaaggcaat aagccgccgg cgacgcacag
    27301 cggaccgggc tggcgcgcca acatccggct cgccgccgac gtcgtcaaca cgctggcaat
    27361 cccgctcgcc acgctcggcg cgacgatcgt cggcgtgcac ctgacgaact cgctcaagga
    27421 acgcgagatc gcgatcgcca ccgagaacaa cgtccaggcc ctgcgcaacc agcgcgagga
    27481 gtccgagacg gcgctgcgct cggcgatgtt ccgggagctg gtcggcccgc tgctgcagtc
    27541 ggaagccgac tcgcgtggca gcgacgcgaa gtcggacctg ccgcgagcgc aacgactcgc
    27601 gctgctcgcc gaactgctca cgctcaactt ccacgagcac ttcgagctcg ggccgctgct
    27661 gcgctacgtg gataccctgc ccggccagac cgacgacgcc cgcatgcgac tgcgcagcac
    27721 ggcgcgacgc gtgatctcgc gacagctcgc accgatgctc gccgtgagcg acggtgccgg
    27781 gcagcacgcc gggttccagg aaatctccct cgatgccgac gtcagtgcgc cgcccgacgc
    27841 gttgctgtcg tgcgcgatcc cggagtccgc ggcccaggcc agcggtgcgc tcacgctcga
    27901 ctgctcgctc gccgcacaag gctcgccact gcgggtgacg tcgccggacg ggcgcgatca
    27961 cctgcagatc agcttccgcg gcgtgagctg gcgcgaccag accgtcggcg tctatgcact
    28021 cgtgaccgac gccaagggcg tgcgggaagc cggtacgccg ccgatcgagt tcacgctgtc
    28081 gccgtactcg ctgccgtact cggacaacac gctgctgtcg agcggcaacc gcttcggcgt
    28141 ctacctcacg cgcgtggacg tgatcccgga cgtggcgcgc atcatgcgcc tgtcgttcgt
    28201 gtggttcccg agggatttcg tgccgccccg cgagcggccc atggacctcg acatcaatcg
    28261 gccggacacc aaggcggaag cgacgaaagg gtaacggaaa atggggacat tcccctattt
    28321 cgagtcgaaa taggggaatg tccccatttc ttcacttgtc cctgatcagg cggcgcagca
    28381 cgggaatgag cagcgcgagc acgacaccga ccgcggtgct gccccagccg agtcgcgcga
    28441 acaggctcgc atactcgggg ttggtggcaa ccgccgtcgt gccggccggc tccgggacca
    28501 tgcccgagaa atcgccggca aacagcgacg cgagcccggt gatgagcatc cagctgccca
    28561 tcatgatgcc ctggtactgg cgcggcgcca gcttgccgat catcgcgtag ccgatcggcg
    28621 agatgagcag ctcgccgatg ctctgcagca cgtagctcag gaacagccag aaaaacgccg
    28681 acttgcccgc cgagtccgcg aacgagattc cgaccggcag cgcgaggaaa cccaggccca
    28741 tcagtatcaa cgaggccgcg aactgccgcg gcaggtcgat gacccagccg cgcgcacgca
    28801 gcctgccgaa cagcacggcg agcaacgggc caccgacggc gatcacgacc gtgttgatgt
    28861 tctgcaccca ctgcggcgca accgcgatgc cccagacgtg caggtccacg ttgttgacgg
    28921 cgaagagctg cagcccgttc ggcgccatct ggtacagcga ccagaacacg agcgacccca
    28981 ccgcgaggac gaggtacgcc ttcatgttgt tcttctcgac cagatccgtg tgccttgcag
    29041 tgagatagat gagccacccc gccaccaggg cgcagaacgc cttgagtgcc gcgcccgtcg
    29101 gaccggcatg ctgcaacatg atgcagacga gcggcgggac gccgaccagg accagcacgc
    29161 cgatcgcgaa gcgcttcctg aactgcggcg ccgtggcccc gagcagcggc gtatcgaggt
    29221 cgcgcagcgc cttccagttg acgcctgcga gcacgatcgc gaggaaattg ccgatggtcg
    29281 cgaacaggaa caggctcgtg tagttctccg tgagctggta gtggccggcc accgcgaacc
    29341 cgacgaagaa gccgacgttc atgcccgcgt aattccagag gaacgccgtc tcgcgccgat
    29401 tgtcggccgg cgcgaagcgc tgcgtgagca tcatgttgag gcaggtgacg ttgagcccac
    29461 tgcccgtgag gaacagtccc agcccccagt agagcgcggt cgtggtcccg agcgcgatgg
    29521 tggcgcagcc gagcacctgc agcaccatgc cgccgacgaa gagattgcgg ttgctcagga
    29581 accggccgcc gagatagccg ccgaacaggt gcagtccgta gttgaacgcc ccgaacatgc
    29641 ccatgaccgc ggtcgcgtcc ctgaccggga acgccaggtg acgcgtcgtg tagagcacga
    29701 gcgtcgagta cagcaccgcg aaccccagcg tcgcgaacac ctggatgaag aacagcgccg
    29761 ccgtgccttc cggaatcctg ctgagcctgg ctgcgatggg gcgcaattcg gtcaccgttc
    29821 gtggggcatg aactgccgga atgatagcgc cacggcagga atcgcggccg ggcaatgccg
    29881 gagcgcgacc cggcgggcgc agcggggatg ggcggcgtat attccggtcg ccggtgcgcg
    29941 ccggcgagcg ggacgacgac gatgtacatc ggcacccacg gcaaggacga ggtgttccgg
    30001 ctgttcggcc agcacgtgtc gagcggcaag gcgaggttct tctcctcggt cggcatcgac
    30061 ttcgtgctcg gccgccgcga gggcccgtgg ctctgggaca tcacgggcgc caggaaactc
    30121 atcgacacgc actgcaacgg cggcgtgttc aacctcggcc accgccaccc cgagatcgtg
    30181 gcgacgatgc gggccgcgct cgacgagctc gacatcggca accaccacct cgtgagcgaa
    30241 cagcgcgccg cgctggccgc gcggatcgcg gcgctgatgc cgggcgacct gtcttacacc
    30301 gtgttcggcg tgagcggcgg ggagtccgtg gacctcgcga tcaaggtcgc gcgggctcat
    30361 acggggcgat ccaagatcgt ctcggccgca ggcgcctatc acggtcacac ggggctcgcg
    30421 ctcgcggccg gcgacgagaa gttccgcgcg ccgttcggcc cgcaggcacc cggcttcgtg
    30481 caggtgccat tcgacgacct cgcggccctc gatcgcgcgg tcgacgatac gaccgctgcg
    30541 gtgatcctcg agacgatccc ggcgaccttc ggcgtgtgcg tgccagccga cggctacctc
    30601 gcaggtgtcc gcgcgctctg cgaccgccgg ggcgtgctgt ggatcgccga cgaggtgcag
    30661 accgggctcg ggcgcaccgg ccggatgtgg ggcttcgagc atgacggcgt cgtgccggac
    30721 atcgtggtgc tgggcaaggg gctctcgggc ggcatctttc ccatgaccgc gacctgcttc
    30781 cgcgctccca tcgagcacgt cttccacgcc gacccgttca tccacgtctc gacgttcggc
    30841 ggcgccgagc tggggtgccc cgtcgcggcg cacgtgctgc aggtcgcgtc ggatccggca
    30901 ttcctgcaaa atgtccgaga cgtggcggcg gtgttcgccg aaggtttccg cgggctggcc
    30961 acgaagcacg aagtgctggt ccgcctgcgc cagcgcgggc tcatgatggg catcgagatg
    31021 gtgaacgagc tgtgcggccc ggtcttcacc aaggcggcct tcgaccacgg actgctgtcg
    31081 atctatgcgg cgaacgaccg gcgcgtcgcg cagctgctgc caccgctcac gaccgatcgc
    31141 agcctggccg aggagatcct gcgacgggtc gatgcagccc tcggggtggt gggcgcgttg
    31201 ctgcggcgat gacggacctc agtgtcgatt cggcccgcct cgacgcgttc gagcacgacc
    31261 tcgacctgcg caatcccgag cgcggcccgg ccgccacgaa agtgctcggc tacggtgaga
    31321 tcagcacggt cctcgcgatc gacgcgccgg gaatgacgca gctcgccttc aagcgaatgc
    31381 ccatgttcct cgacgaaggc gaggccgccc gctacgaagc gctgcacgcc gagtacctgg
    31441 aactgctcaa tgagcgcgtc ggcttgcgcg tggtaccggg ccggaccgtg cggctcgggc
    31501 gccaccgcgg caagatcgtg atgtacatcg tgcaggaacg cctcgcgccg gaccgcgtcg
    31561 cgcagcgcct catcccgcgc ctctccgtag cagggctggc cgccctgaca cgctcggcgc
    31621 tcaccgaggc gggcaaggtg tatgccttca atgcgagtca cgcgcccgcg gtcgaggtcg
    31681 cggtggacgg ccaggtctcc aactgggccc tgcgcgaggc ctccgacgtg gaccgcctcg
    31741 agcgcggcga cacggtgcgg ctcgactacc tcgacacgag cacgccgctg ctgcgcaagg
    31801 gcggccgcga gcaactcgac accgagctgt tcctgcgcag cgcgccgtcg ttcctggtgc
    31861 cgctgctgcg cgccttcatc gtgcaggacg tggtcgaccg ctattacgac ttccggcgtg
    31921 tcgccatcga cgccgtcgcg aaccactaca aggagcagcg cgccgacgcg gtgccgacgc
    31981 tcgtcgacac ggtgaaccgc ttcttcgcgg ccaacgtccc cggcgtcgaa ccgatttcgg
    32041 agaaagcggt gcgcgactac taccgcaccg acgcattcat ctggcgcgtg tacctcgccg
    32101 cccgcaagct cgaccgcaag ctccaccggc tgtcgggccg cgattacccc tacatcctgc
    32161 cggcaaaggt gcggcgctga ccgctcggcc taccaggcgt cgccgaacac ccgcagcagg
    32221 ttcgcgccga tcagcttctc gacgcacgac tcgctgtggc cgcgcgcgag cagcaggccg
    32281 gccagcgtct cgaatcgccg cggggtattg agatcgctcg cgaagaggta gccctcctcc
    32341 gactcgtccg gcgccgcaat gcctcgtgcc ctgcgttcgc ggacgttgtc gcggaagtcc
    32401 gccttgaact cgggcgtgag atcgacggcg gaaagcgtcc cgtccgtgcc tatggagacg
    32461 tggtcctcgc cacagacgtc gagcgcgtgc tcgacgtgcc ggacgacgtc ggccgctgtc
    32521 ggttgccggc ccttcgacag gtacggcatg acgtagatgc cgcagacgcc gcccttctcc
    32581 gccacggc
//
LOCUS       DAOMFD010000005        28467 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_239_length_28467_cov_32.632937, whole genome shotgun sequence.
ACCESSION   DAOMFD010000005 DAOMFD010000000
VERSION     DAOMFD010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 28467)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28467)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28467
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_239_length_28467_cov_32.632937"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2745)
                     /locus_tag="PL152_00710"
     CDS             complement(<1..2745)
                     /locus_tag="PL152_00710"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47820.1"
                     /translation="MAKDKPKSELGALEFRAVGPAIAGRATRVAGVPGDPLTYYVAFA
                     QGGIWKSENGGHEWKPVFDEQSTNSIGSIAIAPSDPNVIYAGSGEANIRGNVSWGTGI
                     FKSSDAGKSWQQVWKTRGQIGTMAVDPRNPDVAFAAVLGSPFGPNKDRGVYRTTDGGK
                     SWKQVLYKNDETGASDVALDPNNPRIVFAGMWQTRRQPWTMTSGGPGSGLYRSADGGD
                     TWEQVKDDGLPKAPWGKVGVRVAPSDSKVVYALIEAKDGGLYRSSDGGETWELASDHR
                     SLRQRAWYYTCLTIDPTNADIVWFPQVPLLKTVDGGKTIVSVDAGIHGDHHDVWIDPK
                     DPRRIMSGNDGGVGVSIDGGATWYWPAIPTPQFYNIDVDDRWPYHVGGTIQDWGTASG
                     PAYTLRRGEHGGGPNLGDNWAVGGGEAGDFVYDRGTPGGVYAGEYGGYISYYQEGVGN
                     FRNVGIYPHDTSGTPAREIKYRFQWTAPIAASPHDPKVLYHGANVLFRTSDHGATWQA
                     ISPDLTRNDPAKQEWSGGPITGDITGVETYDTIFSIAESPLQAGEIWVGTDDGLVQLT
                     RDGGKTWTNVTPKKLPEWGTVEAIEPSRRDAATAYVVVDAHRLDDPHPYLYRTRNHGQ
                     SWEQLGKGLPDDQHLFVVREDPTNANLLYVGAERGVFMSRDGGASFVDIRNNLPAVGV
                     ADIEVKHDDLILGTRRGIWILDDITALRAFDAGVKSEPVHLFAPRKAYRNRLEFRWDD
                     LVDTGSNNAPYGMNVTYWLKDEPAENPAGKKASPADPSELKLEIYDSQGALVRTLSSV
                     PRPDRFPKDDPDEPKDPNKPALTKKQGLNRIQWDLRHEGAQRPGHAKIDAGDPDEGPL
                     VLPGRYTLKLTVAGQVYTTEGEVAADPRSTATLADMRANYEFALRAGAALDRLVADID
                     A"
     assembly_gap    2772..2871
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3174..4895
                     /locus_tag="PL152_00715"
     CDS             3174..4895
                     /locus_tag="PL152_00715"
                     /inference="COORDINATES: protein motif:HMM:TIGR00815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015103 - inorganic anion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HQR47821.1"
                     /translation="MSESAEVSTWSRWLPGVRVLREYRREWLRHDVVAGLVLTTMLVP
                     VGIAYAEASGVPGICGLYATIVPLLAYAVFGPSRILVLGPDSSLAAVILAVVLPLSGG
                     DAQRAIAIAGAMAIVSGVACMAAGLGRLGFVTELLSKPIRYGYMNGIALTVLLSQLPK
                     LFGFKIDAEGPMRQIWAFGRAVFEGRTNAWALAVGIGTVAIILLLQRRPRAPAVLVGV
                     VVATLVAWALDLGERAGVSVLGVLPQGLPSPALPLIGLDDAASVLVGGLAVAMVSFAD
                     TSVLSRSYSARLGTPVNANQEMVGLGIANLAAGFFQGFPISSSSSRTPVAEAAGSRTQ
                     LTGVVGALAITALLLFAPGLLRYLPSPALAGVVIVAAIGLIEVADLRRIYRIQRWEFW
                     LSMTCFAGVAVFGALQGIGIAIVIAVIEFLWDGWRPHAAVLGRVERLPGYHDVKRYPQ
                     ARRVPGLVLFRWDAPLFFANAELFHERVKAAVAASPTPARRVVVAAEPVTSVDVTAAD
                     MLADLHDQLRAEGVELHFAEMKDPVKDKLERFELIARFGTESFHPTVGAAVKDFVRDY
                     EVDWEDR"
     gene            5063..8098
                     /locus_tag="PL152_00720"
     CDS             5063..8098
                     /locus_tag="PL152_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR47822.1"
                     /translation="MSFRKPSTTLRVRQRPATARAAPAEAGARRSWGRLPVREWFESL
                     KLEAQLRVTAVVAVTAALVVAHALIFAWDGLYQRWQDSRAADQLAQSISARLEKPGTH
                     RVFGSLADQPLVLGADVRRDDGKVLERYDAARYARPGESAGRLARWGHATLRFLGLEP
                     GHREYPVKIGGRVTGTLVLVVDNRPPWRAASAGLLLAPLLMACGLLIALRATRAPQRY
                     IVEPLRQLAETTRLGTAGDTPPGDFGGRGNELVDLAANFEALTSRLAAYERDLTNLRV
                     ASRKEVADRTREIEERLARNEAAMSAKDEFLANMSHEIRTPMNGVLGMAELLAGTDLD
                     KRQRRYVDSMRSAAETMMQIINDILDDSKIEAGKMDLVRDPFDVREFAEQVGEIFAGR
                     AESKKLELICRVEPTVPAVVIGDVLRLRQVVGNLVSNAVKYTDRGEIVIRIGVDGQQG
                     DRCRLHFSVQDTGPGIAEADQATVFEAFAQIGNAKRIGGTGLGLSIATRLVKLMGGEK
                     IDLWSRPGEGSSFSFVLPFEVHAAAPAPDRAGDEFGGMRVLVVEDNPTSYMQLEEMLS
                     NWSVDVTVLAQGRLLDDKLREAVARRRPFDVVLLDHALPDASTGDLLRVTRMNPATAS
                     TWVVLLSAFDYDTAYEGTRAIGPDVCIAKPVRQQSLRSALRAARQARAAPADAAAPAA
                     PGRATAGEGPAAMVSLGLDVLVADDNEINREVAQAMLERCGCRVTIAADGNDACEQAG
                     QRRFDAILMDCQMPGMDGYSAAAAIRRAEAERGSRPTAIVALTANVLARDRNRAIEAG
                     MDRFLAKPFTQEQMVAILRPVAEEQGKLVVLPPPAPSRPVARSVTDTHPVVRFEETEE
                     STDLAPAPDESQFDEPLLSDTVVLKMLEMPSGEADESNRLPVLDASQIAAIRGLGKPQ
                     ILERLCELMFATAPETLRRLGEALDAGDLEAVAAAAHALKSPVSSLGGRRLAAALERC
                     EEAALTQRDPEATRRAASGLRQTYDDLEAALRAESGLAA"
     gene            8116..10290
                     /locus_tag="PL152_00725"
     CDS             8116..10290
                     /locus_tag="PL152_00725"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR47823.1"
                     /translation="MTEGRETIILMADDDPAQLMLAEAALAGAGFMVHSAADGAAVVE
                     RFDEVHPDIVVLDVNMPRMSGIDACRAIRERVGSKSLPILMLTGRNDLAAISDAFAAG
                     ASDFAQKGLNPRLLVERVRFLLRDRALQEELRSSRSKLLLAQRVARVGHWELGCDGRS
                     LNVSPVLGEILAVDPASLRSFEDFVRMLDRSEHFAARQAFLACAAGEGRCSSDHRLTT
                     ASGDELWIHLEAERVEQDGQANESTVVLTLQDLTRLRRAEEQARFLSYYDSATGLPNR
                     RHLIEQTVAASAEGAGVAAAGIAAFRIHNLDSIAQSQGIEAAHAMLGHVARLIEIALE
                     NVSEGGMVPWRSASSAVCRTGEAELSLLLRSRVSAQHLFEVATGMLRALAAGMPDELQ
                     DFRPAIGAGLAEIKEGPAGGEQAMLQAHAAAEQAAEPWACDVYSPESQARSRRRLQLE
                     SLLRGAVERGEISLAYQPRVTVDGCVLTGVECLARWENAQVGRVEPREFVPVAERAGL
                     IEPIGEWVLGEACRQLAVWRKHFQLEFRVSVNVSSRQLRDPTLPGTISRLLASHGLPG
                     TALELELTESTAIESAADVRATLQKLRSLGVRVAIDDLGIGLASLTQLRRLTLDTMKL
                     GRSLIGELYTDLGAQGVVTAVIAMARSLQVRSVAVGVTDWETLDMLAALGCDEVQGQY
                     ISLPLGARDFETWLEDGGAASRVRSGRVDLIDAVEAIERRSR"
     gene            complement(10323..>10576)
                     /locus_tag="PL152_00730"
     CDS             complement(10323..>10576)
                     /locus_tag="PL152_00730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47824.1"
                     /translation="ETRMLVWGSATLFAGMALDRYLRKPRAGITSARMTEREGPLEIL
                     QTAGVAALTHGSAAAPAAGEPTLKPGGGRYGGGGASGRY"
     assembly_gap    10577..10676
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10677..11596)
                     /locus_tag="PL152_00735"
     CDS             complement(<10677..11596)
                     /locus_tag="PL152_00735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47825.1"
                     /translation="MSRWNPSPDEERWLAVAARLGSALQRGLVGDRAGGWRTTGTLAR
                     IALFLLGIVAAGLVAGILGIASEQTLLVAGLLASVAAEWLKVTKRMHASGVEEGLCVA
                     GYLMIGAWIAVKVVPHVAATADQVGILIVVLAGAAAGLRLVNPFVTTGAALGLLYWIQ
                     LTGIARTIDSGLGPGTTTIVAGCATAVAALVIGRREFSRPSVDRMLDWLVATLPIAAF
                     AQAPGWIDVGDTSAVVPGAVMRLVRVLLPAALGAVMLTAGLARRRHAPIVGFMGCVVC
                     VAIELHRIFPLPTETRMLVWGSATLFAGMAL"
     gene            complement(11593..12513)
                     /locus_tag="PL152_00740"
     CDS             complement(11593..12513)
                     /locus_tag="PL152_00740"
                     /inference="COORDINATES: protein motif:HMM:NF021446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2157 domain-containing protein"
                     /protein_id="HQR47826.1"
                     /translation="MLREEGALDDATAARASALDRGEIFSLHAELRATMYAGVLLVTG
                     GVGMILARNLDRLGPAAIVLLVALIAVACAVPALRARRAGRDLTIAGDYLLLLAVLLA
                     SADLGYAERQFTLLGPLWPQHLLLLALVHAWVAYAFGSSLVLAASLTALAGWFGVGGG
                     FAHALDVFPSSPELGGRALACAAVITAWRYADRRTRPESRFSDVFDHFATNIAFWGAL
                     AWCLEPGWLLAGLPLVLALSTLSVRRGLTDAREAFVVYGVLYAALGACFVVVPKIHGG
                     TAVMWFVLMVVCAAAVALWQLRRAMREPAP"
     gene            12623..13558
                     /locus_tag="PL152_00745"
     CDS             12623..13558
                     /locus_tag="PL152_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HQR47827.1"
                     /translation="MRISKRDAKAVRSIVRPFRVDKGKGFRLRDFDPGDTLQFGAEDK
                     TRAQEALRKGIEHLAALQDMLYAQDRWSVLLVFQAMDAAGKDGVIKHVLSGVNPQGCQ
                     VTSFKAPSAEELDHDYLWRCMKALPERGRIGIFNRSYYEEVLVVRVHENLLAQQKLPP
                     HLVTPAIWEQRYQDIRAIERYLARNGTLILKFFLNVSKGEQKKRFLERLDDPDKNWKF
                     SMADAAERQHWSDYMSAYEDMIRGTAEPHAPWFVVPADNKWFTRVVVAAAIIDGLQSL
                     RLHYPRVDRAKQKELAAARAELTGTRAPRARRHRG"
     gene            complement(13567..17208)
                     /locus_tag="PL152_00750"
     CDS             complement(13567..17208)
                     /locus_tag="PL152_00750"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional proline dehydrogenase/L-glutamate
                     gamma-semialdehyde dehydrogenase"
                     /protein_id="HQR47828.1"
                     /translation="MTNAVTLDTLLGQFKPNPSSPHAATIQKALMVARHLQERARSLQ
                     TPAERRQQAELDRMLQTPSDKATLALMTDQAFRTNDPARAVEHLTHILDVQGVPRFFG
                     PVDRTLMKGFQSFGSFVPGVALPLVKEQMQKETANVILPGEKEVLTRHLADRRTEGVR
                     MNVNFLGEAILSEVEAERRLQEILQGLQWPEIEVISIKISTLYSQISPLAREHTVATL
                     CDRLERLFRTADRARFTRPDGREVSKFVYLDMEEYRDKELTAETFMRTLDRAGLEHVR
                     AGIALQAYIPDSYRTLLELQAWARRRAASGGGRITIRLVKGANMEAERCESSVRDWPQ
                     APYRTKPETDANYKRMVDELMKPENLAAMDVGIASHNLFDLSYGLVLAHERGALDKVQ
                     FEMLEGMANAQRRALFELTRNLLLYAPACHKENFINAIGYLIRRLDENTGPENFLRHA
                     FKIEVDSPEWHRLEQGFLSSFAAIPHLSHAPRRNQDRNEPPLPTPAVARGWQHFQNEP
                     DTDFALPQNGRWGEDIIAKWLPRCGESAVQVPLVIAGEDLLDGRSVRDCLDPSRPGVV
                     VGRYRQATDADVARAVDCAAADPDGWRTMPDEQRYEILGRVAQELRVARADLMGAALA
                     DGGKTLMESDPEVSEAIDFLEFYRDTARWWRRIPTLKARPKGVVVVVPPWNFPIAIPC
                     GGVAAALAAGNTVIIKPASDTVLVAYEMCQCFWRAGVSKSALQFVPCSGGKEGRKLVN
                     HPKVNAVILTGGTETALTMLGDNPRMNLFAETGGKDATIVTAVSDRDLAIKHILHSAF
                     SHAGQKCSATSLVLLQAEVYDDPAFRNALSEAVQSLKVGSAWELDTKMGPMIKPPSGD
                     LETALKVLEPGEEWAVMPQPADGNPCLWTPGVKYGVQPGSYTHMTEFFGPVLAVMRFE
                     KLGEAVALVNYTGYGLTSGLESLDEREWDYWKRHIRAGNLYINRVTTGAIVLRQPFGG
                     MGKSGFGPGMKAGGPNYVAQFMDFEDVDGMAATGTPQMPTLAALCSGMRAQPQDDDAR
                     IAAAACSYERSEREEFGVEHDHFRLVGQDNVRRYLPVGSVRVRVHDDDSDFDVFARAC
                     AAHVAGCGVVISTPPGLNSPAVRRLEKLTESWAAAIEFVEESDAELAQAIGEGQVDRV
                     RYAAPERASLEVLKSGNAANGCVVSVPVSAEGRLELLWYVREQSISTDYHRYGNLGIR
                     ADEPRAEVL"
     gene            complement(17332..19392)
                     /locus_tag="PL152_00755"
     CDS             complement(17332..19392)
                     /locus_tag="PL152_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47829.1"
                     /translation="MAPSVRHIVVATVLACVAACSGGGGGGGGSKSPARTPLVVNSLF
                     DMETPPAGTVTLRSALAQAASGQAIQFDASLSGGTIALSLVADEHTKLKGEVMGMRNE
                     PSGPVSYLVGYFDRDYGRSALYAHKDVVIDASSLAAGITLAWTGTAPARVLAVYGDLT
                     LRNVAITGGHSVAEQLPTTSPDDQPWTLARGGAVAVWGTAHLVDCRLHGNSVEGDFDQ
                     SRDRGAFGGAVYADIVAMQGCVVSGNSVLGGGAAGGGVYSVGGAESSQSLSTIDRSAI
                     TGNAIRGLYAYGAGAYSDGGGIGKSKTLRVTNSTIARNLAEPAPGLPAFLLRTGYWRG
                     GGIYMSNGSLEVKGATIVENVVRGVARTDSLGRGNLAGGIAATVGNAHAVEGMTIGQS
                     IVAGNTVQEIGGATYEHDLYTGSLMYFHSAGYNRFGVIDFSQILVPVGEPGWASLCRR
                     HFPKQGDASGVALAGVVDLAGGVTVDATIPSAGADAGNPVVLYYSPAGSALAQVPAGT
                     YRVAETLAEYGVSAGATDDFLAIVLGRIEHHYGLAGFAASFTGEFESFLQTADSDAAT
                     AGVQPYRTPHGTAILTLADTQWFGPAQTWPRELYNYPYIEFWHRLDTALGAAGIAGMG
                     PELLGDSAWQALFTSGRLAENQGITVAIQTRDALGVSRLTTDQLGNSRPTQSAADIGA
                     IEAP"
     gene            complement(19508..20473)
                     /locus_tag="PL152_00760"
     CDS             complement(19508..20473)
                     /locus_tag="PL152_00760"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR47830.1"
                     /translation="MSDIHGPCVDPAAFDPARIATTTGAANAALEHLLSTLPTILEVP
                     ASVVRAARAEGKSFFGPIVLSPNGTNRTIDGPAGPLPLRVFATPDARSVLLHIHGGGW
                     SLGSHDQQDVLLDALARSLRLAVVSVGYRLAPEHPYPAGNDDCEAAALWLARNARREF
                     GTDRLLIGGESAGAHLSVTTLLRLRDRHGLSPFRGAYLTYGAFDLGLTPSARSWGERN
                     LVLSTPIIRKFLEWYAPSDLLDPDVSPLHADLRGLPPALFSVGTLDPLLDDSLFMASR
                     WAAGGNAAELAVYPGGIHGFNALPIPLADEANARIARFLDHALGG"
     gene            complement(20526..24098)
                     /locus_tag="PL152_00765"
     CDS             complement(20526..24098)
                     /locus_tag="PL152_00765"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF025376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQR47831.1"
                     /translation="MNADLALPTAAISAHALRTPEPFVRLFARRVQQEGALDLTQGDY
                     KNSDFAPHPEVVRAAQRITRNTVHSYGPAVGRLDVRSEVAEFFNRDGLVDYPGTEVRF
                     MPDEVLFTPGTRAGLAIVLEVLGADGSGVVVPRPSWEYDWFIERAGKKVVELPTSAPE
                     FLPDPADLERLLARGGVSSVILNNPHNPTGRVYPRSLVEEIVRVAVRHRCFVLYDCVY
                     QRLDYVGWFVNPAFANPEWRDHVVSLSGLSKMDMFGASTGARACWLVLSDQIRANGIR
                     ARDVLANLSAWLVATPSTLAQDWALAALQSPLAALRRPSPYMRERRDFMIGAADRLAH
                     LGIERTDFGGTFYAPLAFPGLVGEKFERVRNGVQEQVVVRNSIDAFEFLLSGGVGGIP
                     FAAFAGGTGALDRYGTWQRLSYGSKDVKELAVFMDRVRARIEQQGRLGAAAAAVGPAG
                     SPHDLTWDSPCTAGHYDALDAIDPVGFAEARRRFLADPRHESLRLTGTKHPDSTAHRV
                     EQFERALEAAGGHADRHAHAALTHLEWNPLMDARREYAESIADLLGPCSEVLLDMEGR
                     QISPDWLAVPEKVVLELLRRGVVAGEDRHLLLRAPNPFLEQDEEKVSRILASVARANV
                     LFHLACERLGVVPVRNAIHEITVPQINSRAEIEALLKVGTLYLQAATALLDPASDQHD
                     AFLVRRVGPDRRRSILERLRRVRLVPLCENVGALAHLPDLLEAYYVALGRGVGVDGSP
                     TPAMLSPSFEREEAVVRVFVAMSDTALQSGKLATDSAYALAVGSVAEAEQRLAAAAAS
                     RGLPAPRVTLLVGAGRAGFRGGFDPQHPGVIRQFARAEGVTLQGIRADAPDDAARLAA
                     AFRAACAAGTREPAHAITRADTEALRKVLEGGVQAHTETLLRTAPLLAPFGSLVPQTR
                     VRIRATGSVNYGRSIPSYPAEWTGHLRLPDDRDLRDAWPEGVTLPRAIVYNLACTTLG
                     LPAVTSDLAVLDRRTAVLLERHVPGYREIIATELPAFVKDSVALVFGRKAAETTARRC
                     LRAAAALWIDARPRQDLVAPTCLFAMQYLRYMAEDSECWDETKEHESRTTREEELIRE
                     TSSVAFAPLCGETPGDRWPLLQSMVDSEDAPRLLLARELTIEEKREFVDLRIEGWLRG
                     LPEELSASLRREWAVLRELPGEDFTLDAIRRMLALERLRGSRGA"
     gene            complement(24198..24590)
                     /locus_tag="PL152_00770"
     CDS             complement(24198..24590)
                     /locus_tag="PL152_00770"
                     /inference="COORDINATES: protein motif:HMM:NF017426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF779 domain-containing protein"
                     /protein_id="HQR47832.1"
                     /translation="MVERVSATPEALELIAVLRGQHGDLMFHQSGGCCDGSSPMCYPR
                     GEFLVGNSDVLLGEIGGCPFYMSRAQFEYWRHTHLTIDVVPGRGGMFSLEGPTGRRFL
                     TRSRLFTDDEARLLETAEPAPAAAAAPR"
     gene            complement(24590..26110)
                     /locus_tag="PL152_00775"
     CDS             complement(24590..26110)
                     /locus_tag="PL152_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR47833.1"
                     /translation="MRYLDPGTAGAKVSFKSRYENFIGGKWVAPVRGQYFDNVSPVNG
                     KVFCQVPRSTEEDINLALDAAHAAADAWGSTPVAQRAAILNKIADRIEANLEMIAIAE
                     TWDNGKPVRETLAADIPLAVDHFRYFAACVRAQEGTLAEIDESTVAYHFHEPLGVVGQ
                     IIPWNFPILMATWKLAPALAAGNCVVLKPAEQTPVGILLVAELIADLLPPGVLNIVNG
                     FGVEAGKPLASSSRIAKIAFTGETTTGRLIMQYASQNLIPVTLELGGKSPNIFFEDVM
                     AEDDDFLDKSLEGFTLFALNQGEVCTCPSRALVQESIADKFLERVVARVKKIKQGHPL
                     ETDTMVGAQASSEQMEKILSYLDIGRKEGAQVLTGGGKAVVGSDIASGYYIEPTVFRG
                     HNRMRIFQEEIFGPVVSVTTFKDEADALKIANDTLYGLGAGVWSRDGSRAYRMGRGIK
                     AGRVWTNCYHLYPAHAAFGGYKQSGIGRETHRMMLDHYQQTKNLLVSYSPKALGFF"
     gene            complement(26267..27052)
                     /locus_tag="PL152_00780"
     CDS             complement(26267..27052)
                     /locus_tag="PL152_00780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQR47834.1"
                     /translation="MLELLLDPQAWLAFATLAALEIVLGIDNLIFIAILAGKLPHEQQ
                     ARARRMGIAAALVSRLMLLAALAWIMKLTEPLFTVAATDISGRDLVLIVGGAFLLYKA
                     TREIHEKLEGAAAPSAPAAAASTAFAAVIGQIMVIDVVFSLDSIITAVGMVDEIEIMV
                     AAILTAVIFMLVFAESVHGFVNRHPTIKMLALAFLLLIGVALVADGAGFHIPKGYIYG
                     SMAFATLVELLNIRLRRRGAPVRLHEPHVVPERRCEACGQTLP"
     gene            complement(27080..28003)
                     /locus_tag="PL152_00785"
     CDS             complement(27080..28003)
                     /locus_tag="PL152_00785"
                     /inference="COORDINATES: protein motif:HMM:NF014433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HQR47835.1"
                     /translation="MDATRVQVFIGKGGVGKTTCSAATALALAERGDSSLVISTDPTP
                     SLSHIFEIAGGHRERELLPRLYLTELGLKDVRNLWDERFGRDVYGVFSSFVDVEYTTF
                     VEFVTAVLPGLNEEFMVDYIRRLAIESRFRHLVWDTAPLGQTLALLGMPALLAEHLRA
                     APRIYSHLRTTGERREPVIAILQRWRDLAAECMEFLRRDVAFTMVAIPEALSVYQLED
                     AVGELARYGLRMSRLVINNVVQDAPSAFLQQKRREQQSHLLRIRDRYGSVPIVEVPLF
                     PGEVRGFARLRAVGRLLVPQDAPAAQRDAVP"
     gene            28179..>28467
                     /locus_tag="PL152_00790"
     CDS             28179..>28467
                     /locus_tag="PL152_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR47836.1"
                     /translation="MSVRCNRRAFCQAIGAAAAIAAMRSARSQPEESVMLERPIPSTG
                     ERLPVIGLGTSDVFDVGPDPAARKPLHEVLDILLGSGGSLLDTSPMYGRAEQ"
BASE COUNT         4257 a         9636 c         9961 g         4413 t
ORIGIN      
        1 ggcgtcgatg tccgccacga ggcgatcgag ggccgctccg gcgcgcaagg cgaactcgta
       61 gttcgcgcgc atgtcggcca gggtcgcggt cgagcgcgga tcggcagcga cctcgccctc
      121 ggtcgtgtag acctgccccg caaccgtcag cttcagggtg tatctgcccg gcagcacgag
      181 cggaccctcg tccggatcgc ccgcatcgat cttcgcgtgc ccgggccgtt gcgcgccttc
      241 gtggcgcagg tcccactgga tgcggttcaa cccctgcttc ttcgtgagcg ccggcttgtt
      301 cgggtccttc ggctcgtccg gatcgtcctt cgggaaccgg tcgggcctgg gcaccgagct
      361 cagcgtacgt acgagggcgc cctgcgagtc gtagatctcg agcttgagct cggacggatc
      421 ggcagggctc gctttcttgc cggccggatt ctccgccggc tcgtccttga gccagtacgt
      481 gacgttcatg ccgtagggcg cattgttgga gccggtgtcg acgagatcgt cccagcggaa
      541 ctccagtcgg ttgcgatacg ccttgcgcgg cgcgaagagg tgcacgggct cggacttcac
      601 gccggcatcg aacgcgcgca gggcggtgat gtcgtcgagg atccagatgc cgcggcgcgt
      661 gccgaggatc aggtcgtcgt gcttgacctc gatgtccgcg actccgacgg ccggcaggtt
      721 gttacggatg tcgacaaagc tcgcgccgcc gtcgcgcgac atgaagacgc cccgttccgc
      781 gccgacgtac aggaggttcg cgttggtcgg gtcttcgcgg acgacgaaca ggtgctggtc
      841 gtcgggcagc cccttgccga gttgctccca gctctggccg tggtttcgcg tgcggtaaag
      901 gtacggatgg ggatcgtcga gacggtgcgc gtccacgacc acgtaagccg tcgccgcgtc
      961 gcgtcgcgag ggctcgattg cctcgaccgt gccccactcg ggcagcttct tcggcgtgac
     1021 gttcgtccac gtcttgccgc cgtcgcgcgt gagctgcacg aggccgtcgt cggtgccgac
     1081 ccaaatctcc cctgcctgaa gcgggctctc cgcgatggag aagatcgtgt cgtaggtctc
     1141 gacgccggtg atgtcgccgg tgatcgggcc gcccgaccat tcctgcttgg ccgggtcgtt
     1201 gcgcgtgagg tcggggctga tcgcctgcca agtcgcaccg tggtccgagg tgcggaacag
     1261 cacgttcgcg ccgtgataca gcaccttggg atcgtgcggc gaggccgcga tcggcgcggt
     1321 ccactggaag cggtacttga tttcccgggc gggagtgccc gacgtgtcgt gcggatagat
     1381 gccgacgttg cggaaattgc ccacgccctc ctggtagtac gagatgtagc cgccgtactc
     1441 gccggcgtac acgccgccgg gcgtgccgcg gtcgtacacg aagtcgccgg cctcgccgcc
     1501 gcccaccgcc cagttgtcac ccaggttcgg gccaccgccg tgctcgccgc ggcgcaacgt
     1561 gtaggcgggg ccgctcgccg tgccccagtc ctggatcgtt ccgccgacgt ggtacggcca
     1621 gcggtcgtcc acgtcgatgt tgtagaactg cggggtcggt atggcaggcc agtaccaggt
     1681 ggcgccgccg tcgatggaca cgccgacgcc gccatcgttg ccggacatga tgcggcgcgg
     1741 atccttcggg tcgatccaca cgtcgtggtg gtcgccgtgg atacccgcgt cgaccgacac
     1801 gatggtcttg ccaccgtcca ctgtcttgag cagcggtacc tgcgggaacc agacgatgtc
     1861 cgcgttggtc gggtcgatcg tcaggcacgt gtagtaccac gcgcgctggc gcagcgagcg
     1921 atggtcgctc gcgagttccc aggtctcgcc gccgtcgctc gagcgataca ggccaccgtc
     1981 cttcgcctcg atgagcgcgt agaccacctt cgagtcggac ggcgcgaccc gcacgccgac
     2041 cttgccccac ggcgccttcg gcaggccgtc gtccttgacc tgctcccacg tgtcgccgcc
     2101 gtccgccgag cggtacaggc cgctgcccgg cccgccgctc gtcatcgtcc acggctggcg
     2161 tcgcgtctgc cacatccccg cgaacacgat gcgcggattg ttcgggtcga gcgcgacgtc
     2221 cgatgcgccc gtctcgtcgt tcttgtagag gacctgcttc caggatttgc cgccgtcggt
     2281 cgtgcgatag acaccgcggt ccttgttcgg gccaaacggt gaacccagca ctgcggcgaa
     2341 cgcgacgtcc ggattgcgcg ggtcgacggc catcgtgccg atctgtccgc gcgtcttcca
     2401 gacctgctgc cagctcttgc ccgcatccga agacttgaag atgccggtgc cccaggagac
     2461 gttgccgcgg atgttggctt cgccgctgcc ggcatagatc acgttcgggt cggacggggc
     2521 gatcgcgatc gacccgatcg agttggtgga ctgctcgtcg aacaccggct tccattcgtg
     2581 cccgccgttt tcgctcttcc agatcccacc ctgcgcgaac gcgacgtagt aggtgagggg
     2641 gtcgcccggc acgccggcca cgcgcgtcgc acgcccggcg atcgcgggac cgaccgcgcg
     2701 gaattcgagc gccccaagtt cggacttggg cttgtccttc gccatcgctg gcgcggacac
     2761 gactgcaagc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagttcggac
     2881 ttgggcttgt ccttcgccat cgctggcgcg gacacgactg caagcactac gagagccagg
     2941 atggcgcgcg agtagcgcgc atgcgtgtga ttcacgggac tcctaaccgt tggcacgggt
     3001 ccggtttgtt gttacagcat cgtagcgcgc cgcctgcgtg ccgtacaccg gcggcgggat
     3061 cctgcgccgc gcggtcacga gtcgacggtg cggccggggc actctccgcg gactgtgtgc
     3121 gcacggcgca ccggcagcta tgattgccga ggtacaccgc ttgcgagtcg tcgatgtcgg
     3181 aatccgccga ggtatcgacc tggtcacgct ggctgcccgg cgtccgggtg cttcgcgagt
     3241 accgccgcga gtggttgcgg catgacgtcg tcgccggcct ggtgctcacc acgatgctgg
     3301 tacccgtcgg gatcgcctat gcggaggcat cgggtgtgcc gggcatttgc ggcctgtacg
     3361 cgacgatcgt gccgttgctc gcgtacgccg tgttcgggcc cagccggatc ctggtgctcg
     3421 ggccggattc gtcgctcgcg gcggtcatcc tcgcggtcgt gctgccgctg tcgggcggcg
     3481 acgcgcagcg cgcgattgcg atcgccggcg cgatggccat cgtctccggc gtcgcatgca
     3541 tggccgccgg actcggtcgc ctcgggttcg tgaccgaact gctgtccaag ccgatccgct
     3601 acggatacat gaacggcatc gcgctcaccg tgctcctgag ccaattgccc aagctcttcg
     3661 gcttcaagat cgacgcggaa ggtcccatgc gccagatctg ggcgttcggg cgcgccgtgt
     3721 tcgagggccg aaccaatgcc tgggcactgg ccgtcggaat tggaaccgtg gcgatcatcc
     3781 tgttgctgca gcggcgtccg cgcgcacctg cggtcctggt cggcgtggtc gtcgccaccc
     3841 tcgttgcctg ggcactcgac ctcggcgagc gggcgggcgt gtccgtgctc ggcgtgctgc
     3901 cccagggcct gccgtcgccg gcattgccgc tgatcgggct ggacgacgcc gcttccgtgc
     3961 tggtcggtgg actcgccgtc gccatggtct cgttcgccga cacgagcgtg ttgtcgcgtt
     4021 cctactcggc acggctgggg actccggtga acgccaacca ggagatggtg gggctcggta
     4081 tcgcgaacct ggcggcgggc ttcttccagg gcttcccgat cagcagcagt tcgtcgcgca
     4141 ctccggtggc ggaagcggcg ggttcccgca cccagttgac gggcgtcgtc ggcgcgctgg
     4201 ccatcaccgc gctgctgctc ttcgcgccgg gcctgctccg gtacctgccg agcccggcgc
     4261 tggccggggt cgtgatcgtc gccgcgatcg ggctcatcga ggtcgcggac ctgcgacgca
     4321 tctatcgcat ccagcgctgg gagttctggc tgtcgatgac ctgcttcgcc ggtgtcgcgg
     4381 tgttcggcgc gctgcagggc atcggcatcg cgatcgtgat cgccgtgatc gaattcctgt
     4441 gggacggctg gcgaccgcat gcggcggtgc tcgggcgcgt ggagcgcctg cccgggtatc
     4501 acgacgtgaa acgctacccg caggcgcggc gcgtcccggg gctggtgctg ttccgatggg
     4561 atgcgccgct gttcttcgcc aatgcggagc tgttccacga gcgcgtgaag gccgcggtcg
     4621 ctgcgtcgcc gaccccggcg cgccgtgtcg tcgtggccgc ggagcccgtc accagcgtgg
     4681 acgtcacggc cgcggacatg ctggcggacc tgcacgacca gttgcgcgcc gagggagtcg
     4741 agctgcactt cgccgagatg aaggatccgg tcaaggacaa gctcgagcgt ttcgagctca
     4801 tcgcgcgatt cggcacggaa agcttccacc ccaccgtcgg ggcggcggtc aaggacttcg
     4861 tgcgcgacta cgaggtcgac tgggaggacc gctagcggtc ctcggtaccg gccgcaagcg
     4921 ccgcccgccg caagccgggc cccggtgccc gcgggacttg cgccacgtca cagtcctgca
     4981 ggctgctcgt accccttatg tcacgttccc gcttaggctg tcgtgcaatt cagtgccttc
     5041 cgtcgggagc cacctgcgcg cgatgtcatt caggaagcca tcgaccaccc tgcgtgtccg
     5101 gcaacgcccg gccaccgcgc gtgccgcgcc ggcggaggcg ggtgcgcggc gctcgtgggg
     5161 gcggctgcct gtccgcgagt ggttcgagtc actgaagctg gaggcgcagc tgcgggtcac
     5221 ggccgtcgtg gcggtgactg cggcgctggt ggtcgcgcat gcgctcatct tcgcgtggga
     5281 tgggctgtac cagcgctggc aggacagccg ggccgccgac cagctcgccc aatcgatctc
     5341 ggcgcgcctg gagaagcccg ggacgcaccg ggtgttcggg tcgctcgcgg accagcccct
     5401 ggtcctgggt gccgacgtcc gtcgcgacga cggcaaggtg ctggagcgct acgacgcggc
     5461 gcgctacgcc cggccaggtg agtccgcagg gcgcctcgca cggtgggggc atgcgacgct
     5521 gcgcttcctc ggtctcgagc cgggccaccg cgagtacccg gtcaagatcg gcggaagggt
     5581 gaccggcacg ctggtgctgg tcgtcgacaa ccggccgccc tggcgggcgg ccagcgccgg
     5641 cctgctgctt gcgcctttgc tgatggcgtg cggcctgctg attgcgctgc gggccacccg
     5701 cgcgccgcag cgctacatcg tcgagccgct gcgccagctg gcggagacga cccgcctcgg
     5761 gacggcgggc gatacgccgc ccggcgattt cggaggacgc ggcaacgagc tcgtcgacct
     5821 ggccgccaac ttcgaggcct tgacgagccg gctcgccgcg tacgagcgcg acctgacgaa
     5881 ccttcgcgtg gcaagccgca aggaggtggc cgacaggacc cgggagatcg aggagaggct
     5941 cgccaggaac gaggctgcga tgagcgccaa ggacgaattc ctggcgaaca tgagccacga
     6001 gatccgcacg cccatgaacg gcgtgctcgg catggccgag ctgctggcgg gcaccgacct
     6061 cgacaagcgc cagcgccgct acgtggactc gatgcgctcc gccgccgaga cgatgatgca
     6121 gatcatcaac gacatcctcg acgactcgaa gatcgaggcc ggcaagatgg acctcgtgcg
     6181 cgacccgttc gacgtccgcg aatttgccga gcaggtgggg gaaatcttcg ccggccgcgc
     6241 cgaaagcaag aagctcgagc tgatctgccg tgtcgagccc accgtccccg cggtcgtcat
     6301 cggggacgtg ctgcgcttgc ggcaggtggt cggcaacctg gtgtcgaatg ccgtcaagta
     6361 caccgaccgg ggcgagatcg tgatccgcat cggcgtggac ggccagcagg gcgatcgctg
     6421 ccggctgcac ttcagcgtgc aggacaccgg accgggcatc gcggaggctg accaggcgac
     6481 ggtgttcgag gccttcgccc agatcggcaa tgcgaagcgc atcggtggca cgggtctcgg
     6541 cctgtccatc gccacccgcc tcgtgaagct catgggcggc gagaagatcg acctgtggag
     6601 ccggcccggc gagggcagct ccttctcctt cgtgctgccg ttcgaggtgc acgcggcagc
     6661 gccggcgccc gatcgcgccg gcgacgagtt cgggggaatg cgggtgctgg tcgtggaaga
     6721 caacccgacc agctacatgc agctcgagga aatgctttcg aactggtcgg tggacgtcac
     6781 cgtgctcgcg caggggcggc tcctcgacga caagttgcgc gaagccgtcg cgcggcgacg
     6841 gccgttcgac gtcgtcctgc tcgaccacgc gctgccggac gcatccaccg gtgacctgct
     6901 gcgggtgacc cgcatgaacc ctgcgaccgc atccacgtgg gtggtgctgt tgagcgcctt
     6961 cgactacgac acggcctacg agggcacgcg cgcgatcggc ccggacgtgt gcatcgcgaa
     7021 gccggtccgg cagcagtcgc tgcgcagcgc actgcgggcc gcgcgccagg cgcgcgcggc
     7081 gcctgcggac gcagcagcac cggcggcacc gggtcgggcg acggcgggcg aaggtcctgc
     7141 cgccatggtc tcgctcgggc tggacgtgct cgtcgccgac gacaacgaga tcaaccgcga
     7201 ggtggcgcag gcgatgctcg agcgatgcgg ctgccgggtg acgattgcgg ccgacggcaa
     7261 cgatgcctgc gagcaggcag ggcagcgtcg gttcgacgca atcctcatgg attgccagat
     7321 gccgggcatg gacggataca gcgccgccgc ggcgatccgg cgggccgagg ccgagcgagg
     7381 gtcccggccg acggccatcg tggcgctgac ggccaacgtg ctcgcccgcg atcgcaaccg
     7441 ggccatcgag gccgggatgg accgcttcct ggcgaagccg ttcacgcagg agcagatggt
     7501 cgccatcttg aggccggtcg ccgaggagca gggcaagctc gtggtcttgc cgccacccgc
     7561 accgtcgcga ccggtggcac gaagcgttac cgacacgcat ccggtcgtgc ggttcgaaga
     7621 gaccgaggag tcgacggatc tcgcgccggc gccggacgaa tcccagttcg acgagccgct
     7681 gctctcggac acggtcgtgc tgaaaatgct cgagatgccc tcgggcgagg ccgatgagtc
     7741 gaatcgcctg ccggtgctgg acgccagcca gattgccgcc attcgcgggc tggggaagcc
     7801 gcagatactc gagcgattgt gcgagctcat gttcgccact gcccccgaga cactgcgcag
     7861 gctcggggag gcgctcgacg ccggggacct ggaggcggtc gcggcagcgg cgcacgccct
     7921 gaagtcgccc gtgagcagcc tcggggggcg gagactcgcg gccgcgctcg agcgctgcga
     7981 ggaagccgcg ctcacgcaac gtgacccgga agcgactcgc cgggccgcga gcggactgcg
     8041 gcagacctac gacgacctcg aggctgcgct gcgcgcggag tcggggctcg ctgcctgatt
     8101 gcggaggatc gggagatgac cgaagggcgc gagacgatca tcctgatggc cgacgacgac
     8161 ccggcgcagc tgatgctcgc ggaagcggca ctcgcgggcg cgggattcat ggtgcactcc
     8221 gcggccgacg gtgctgcggt cgtcgaacga ttcgacgagg ttcatcccga catcgtcgtg
     8281 ctcgacgtca acatgccgcg gatgtccggc atcgacgcct gccgtgccat ccgcgagcgc
     8341 gtcggctcca agtcgctgcc catcctgatg ctcaccggtc gcaacgacct ggccgcgatc
     8401 agtgacgcgt tcgcggcagg cgccagcgat ttcgcccaga agggcctgaa cccgcgcctg
     8461 ctggtggagc gggtgcgctt cctgttgcgc gatcgcgcct tgcaggagga gctgcgctcc
     8521 agccgctcga agctgctgct cgcgcagcgc gtggctcgcg tcgggcactg ggaactcggc
     8581 tgcgacgggc gcagcctgaa tgtctcgccg gtgctcggcg agatactggc tgtcgatccg
     8641 gcgtcgctgc gctccttcga ggatttcgtg aggatgctcg accggtcgga gcacttcgcg
     8701 gcacgccagg cgttcctcgc ttgtgcggcc ggcgaaggac gctgcagttc cgatcatcgc
     8761 ctgacgacgg catccggtga tgaactctgg atccacctgg aggcggaacg cgtcgagcag
     8821 gacggacagg cgaacgagtc gacggtggtc ctcacgttgc aggacctcac gcggttgcgc
     8881 cgcgcggaag agcaggcacg atttctttcc tattacgact ccgcgaccgg cctgccgaac
     8941 cgacggcacc tgatcgagca gacggtcgcg gcttccgccg aaggtgccgg ggttgcggct
     9001 gcaggcattg ccgcattccg gatccacaac ctcgattcca tcgcccagtc gcagggcatc
     9061 gaggccgcgc acgccatgct cggccacgtg gcccggctca tcgagatcgc gctcgagaac
     9121 gtcagcgagg gtggcatggt gccgtggcgc tcggcgtcgt cggccgtttg ccgtaccggc
     9181 gaggcggaac tctccctgct gctgcgcagc cgggtttccg cccagcacct gttcgaggtg
     9241 gcgaccggca tgctgcgcgc gctggccgcc ggcatgccgg acgaactgca ggatttccgc
     9301 ccggcgatcg gtgccgggct ggccgagatc aaggaaggcc cggccggtgg cgagcaggcg
     9361 atgctgcagg ctcacgcagc ggccgagcag gcggcggaac cctgggcctg tgacgtctat
     9421 tcacccgaat cgcaggcgcg ttcacgccgg cggctgcagc tcgagtcgtt gctgcgcggc
     9481 gcggtggagc gcggcgagat ctcgctcgcg taccagccgc gcgtcaccgt cgacggctgc
     9541 gtgctcaccg gcgtggaatg cctggcgcgc tgggaaaacg cgcaggtcgg tcgcgtggag
     9601 cccagggagt tcgtcccggt cgccgagcgg gcggggctga tcgagccgat cggtgaatgg
     9661 gtgctcggcg aggcctgtcg gcagctcgcc gtctggcgca agcacttcca gctcgagttc
     9721 cgggtctcgg tgaacgtgtc gtcgcgacag ctgcgcgacc cgacgctgcc ggggacgatc
     9781 agccgcctgc tggcgagcca cggcctgccg gggaccgctc tcgagctgga gctgaccgag
     9841 tcgacggcga tcgaatcggc cgcggatgtc cgcgcgacgc tgcagaagct gcgctcgctc
     9901 ggcgtccgcg tggccatcga cgacctgggc atcgggcttg cgtcgctgac ccagctgcgg
     9961 cgtctcacgc tggacaccat gaagcttggt cgctcactga ttggagagct ctacaccgat
    10021 cttggcgcgc agggcgtcgt cacggcggtg atcgcaatgg cgcgcagtct tcaggtgcga
    10081 tcggtggccg tgggcgtcac cgactgggag acgctggaca tgctggccgc gctggggtgc
    10141 gacgaggtgc aggggcagta catatcgcta ccgctcggcg cgcgtgattt cgaaacctgg
    10201 ctcgaggacg gcggtgcggc atcgcgggtc cgcagcggac gggtggacct gatcgacgcg
    10261 gtcgaggcga tcgagcgtcg ctctcgctga cgcccgcgcg tcgatccgcg tggcgacggc
    10321 gctcagtacc ggccgctcgc accgccgccg ccgtagcgcc cgccgccggg cttgagcgtg
    10381 ggctcgcctg cagccggagc cgcggcgctg ccgtgtgtga gcgcggcgac gcccgccgtc
    10441 tgcaatatct ccaagggacc ctcacgctcg gtcatcctcg ccgaggtgat gcccgcgcgc
    10501 ggcttgcgca agtagcggtc gagggccatt ccggcgaaca acgtggcgct tccccagacg
    10561 agcatccggg tctcggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaggg
    10681 ccattccggc gaacaacgtg gcgcttcccc agacgagcat ccgggtctcg gtgggcaggg
    10741 gaaatatgcg gtgcaattcg attgcgacgc agacgacgca gcccatgaag cccacgatcg
    10801 gcgcgtgacg ccggcgcgcc agcccggccg tcagcatcac cgcgccgagc gcggccggca
    10861 gcaggacgcg cacgagccgc atcacggcgc ccgggacgac cgcgctcgtg tcgccgacat
    10921 cgatccaccc cggagcctgg gcgaaggcgg caatcggcag ggtggccacc agccagtcca
    10981 gcatccggtc gacggatggg cggctgaact ctcgcctgcc gatcaccagc gccgccaccg
    11041 ccgtcgcgca gcctgcgacg atcgtcgtcg tgccgggtcc gaggccggag tcgatggtcc
    11101 gcgcgatccc ggtcagctgg atccaataca gcagcccaag tgcggcgccg gtcgtcacga
    11161 acggattgac caggcgcaac ccagccgcgg caccggccag gacgacgatc aggattccga
    11221 cctggtcggc cgtcgcggcg acatgcggca cgaccttgac cgcgatccag gcgccgatca
    11281 tgaggtagcc ggcgacacac aagccctctt cgacacccga agcgtgcatg cgcttcgtga
    11341 ccttgagcca ctcggctgcg acgctggcga gcagccccgc cacgagcagt gtctgctcgc
    11401 tggcgatgcc caggattccg gcgacgagcc cggcggcgac gatgccgagc aggaagagcg
    11461 cgatgcgcgc aagtgtgccg gtcgtgcgcc atccgccggc gcgatcgccc acgaggccgc
    11521 gctggagggc gctgcccagt cgcgcggcaa cggccagcca gcgctcctcg tccggcgacg
    11581 gattccagcg gctcacggtg ccggctcgcg catggcgcgg cgcagctgcc agagcgcaac
    11641 tgccgcggcg cacacgacca tcaggacgaa ccacatcacc gccgtcccgc cgtggatctt
    11701 cggcacgacc acgaagcagg ccccgagcgc cgcgtacagg acgccgtaga ccacgaatgc
    11761 ctcgcgggca tcggtgagtc cgcggcgcac ggagagggtc gacagcgcca gcaccagcgg
    11821 cagcccggcc aacagccatc cgggctccag gcaccacgcc agcgcgcccc agaacgcgat
    11881 gttggtcgcg aagtgatcga acacgtcgct gaaccgcgac tccggtcgcg tgcgccggtc
    11941 ggcgtagcgc caggcggtga tgacggcggc acaggcgagc gcgcgaccgc ccagctccgg
    12001 ggacgaggga aacacgtcga gcgcgtgcgc gaagccgccg ccgacaccga accagccggc
    12061 cagtgccgtg agcgacgccg cgagcaccag cgacgacccg aacgcgtagg caacccacgc
    12121 gtggaccagg gccagcagca gcaggtgctg gggccacaac gggccgagga gcgtgaactg
    12181 ccgttccgcg tagccgaggt ccgcgctcgc gagcagcacg gcgagcagca ggaggtaatc
    12241 gccggcgatg gtgaggtcgc ggccggcgcg ccgggcccgc agggccggca ctgcgcacgc
    12301 gaccgcgatc agcgccacga ggagcacgat ggctgccggt cccaggcgat cgaggttgcg
    12361 cgccaggatc atgccgacgc cgccggtcac cagcagcacg ccggcataca tcgtcgcccg
    12421 cagttccgca tgcagcgaga agatctcccc gcggtcgagt gccgaggccc gcgcggccgt
    12481 cgcgtcgtcg agcgcccctt cttcccgcag catccgcaat tctggttcga gcgagtgcat
    12541 gcggacactg tagcgccgat gccgccgctt gggcagctgc cggcctatac tggctccggc
    12601 ccaacgcgag gagggagacg cgatgcggat atcgaaacgc gatgcgaagg ccgtgcggtc
    12661 catcgtgcgg ccgttccgcg tcgacaaggg caagggattc cgcctgcgtg acttcgatcc
    12721 cggcgacacg ctgcaattcg gcgcggagga caagacgcgc gcgcaggagg cgctgcgcaa
    12781 gggcatcgag cacctcgcgg cgctccagga catgctgtac gcgcaggatc gctggtcggt
    12841 gctgctcgtc ttccaggcga tggacgctgc cggcaaggac ggtgtcatca agcacgtcct
    12901 gtccggcgtc aatccacagg gctgccaggt cacgtcgttc aaggcgccgt cggccgagga
    12961 gctcgaccac gactacctgt ggcgctgcat gaaggcgctg ccggagcggg ggcgcatcgg
    13021 catcttcaat cgctcgtatt acgaggaagt cctggtcgtg cgcgtgcacg agaacctgct
    13081 cgcacagcag aagctcccgc cgcatctggt cacgcccgcg atctgggagc agcgctacca
    13141 ggacatccgt gcgatcgagc gctacctggc gcgcaacggc accctgatcc tcaagttctt
    13201 tctcaacgtc tcgaagggtg agcagaagaa gcgcttcctc gagcgcctcg acgacccgga
    13261 caagaactgg aagttctcga tggcggacgc cgcggagcgc cagcactgga gcgattatat
    13321 gagcgcctac gaggacatga tccgcggcac cgcggagccg catgcgccct ggttcgtcgt
    13381 tcccgccgac aacaagtggt tcacgcgcgt cgtcgtggct gcggcgatca tcgacggact
    13441 gcagtccctg cgcctgcact accctcgcgt ggaccgcgcc aagcagaagg aactcgccgc
    13501 tgcgagggcc gagctcaccg gcacccgcgc gccgcgtgcg cgacggcacc gaggctgatc
    13561 agcgcgtcaa agcacctcgg cccgcggctc gtcggcccgg atgccgaggt tgccgtagcg
    13621 gtggtagtcg gtgctgatgc tctgctcgcg cacgtaccac agcagctcga gacgaccttc
    13681 ggccgatacc ggcacgctga ccacgcaacc gttcgcggcg ttgcccgact tcaggacctc
    13741 gagcgacgcg cgctccgggg ccgcgtaacg cacgcggtcc acctgtccct cgccgatcgc
    13801 ctgcgcgagt tcagcatcgg actcctccac gaactcgatc gctgcggccc aggattccgt
    13861 cagcttctcc agtcgccgga cagccggcga attcaggcct ggcggcgtgc tgatgaccac
    13921 gccgcagccg gccacgtgcg ccgcgcaggc cctcgcgaac acgtcgaagt ccgaatcgtc
    13981 gtcgtggacg cggacccgca cgctgccgac gggcaggtag cggcgcacgt tgtcctggcc
    14041 gaccagcctg aagtgatcgt gctcgacgcc gaactcttcc cgctcgctgc gctcgtagct
    14101 gcaggcggcg gccgcgatgc gcgcatcgtc gtcctgcggt tgtgcccgca tcccggagca
    14161 cagcgccgcg agcgtcggca tctgtggcgt gccggtcgcc gccatgccgt cgacgtcctc
    14221 gaagtccatg aactgggcga cgtaattcgg cccgccggcc ttcatgccgg gtccgaagcc
    14281 cgacttgccc atgccgccga atggttgccg gagcacgatg gcgccggtgg tgacgcggtt
    14341 gatgtacagg ttgccggcgc ggatgtgcct cttccagtag tcccactcgc gctcgtcgag
    14401 actctcgagg ccgctggtga gtccgtagcc cgtgtagttc acgagtgcga cggcctcgcc
    14461 gagcttttcg aagcgcatca cggcgagcac gggaccgaag aactcggtca tgtgcgtgta
    14521 gctgcccggc tgcacgccgt acttgacgcc gggagtccat agacacgggt ttccatcggc
    14581 tggctgaggc atgacggccc attcttcgcc aggctccagg accttgagcg cggtctcgag
    14641 gtcgcccgac ggcggcttga tcatcggtcc catcttcgtg tcgagttccc acgcggagcc
    14701 gaccttcagg ctctgcaccg cctccgacaa cgcattgcgg aatgccgggt cgtcgtacac
    14761 ctcggcctgc agcagcacca gcgacgtcgc cgagcacttc tggccggcgt ggctgaatgc
    14821 cgagtgcagg atgtgcttga tcgcgaggtc gcggtccgac acggccgtga cgatcgtggc
    14881 gtccttgccg ccggtctcgg cgaacaggtt catcctcggg ttgtcgccga gcatcgtgag
    14941 cgccgtctcc gtgccgcccg tcaggatcac tgcgttcacc ttcgggtggt tgacgagctt
    15001 gcgcccctcc ttgccgcccg agcagggcac gaactgcaga gcgctcttcg acacgccggc
    15061 gcgccagaag cactggcaca tctcgtacgc gacgaggacg gtgtcagagg ctggcttgat
    15121 gatcacggtg ttgccggccg cgagcgccgc cgcgacgccg ccacagggga tcgcgatcgg
    15181 gaagttccag ggcggcacga cgaccacgac acccttggga cgcgccttca gcgtcgggat
    15241 cctgcgccac cagcgcgccg tgtcgcggta gaactcgagg aagtcgatcg cctccgacac
    15301 ctcggggtcg gactccatca gtgtcttgcc gccatcggcg agcgcggcgc ccatgaggtc
    15361 ggcgcgcgcg acgcgcagtt cctgagcgac gcggccgagg atctcgtagc gctgctcgtc
    15421 cggcatggtg cgccaaccgt ccggatccgc ggccgcgcaa tcgaccgcgc gtgcgacgtc
    15481 ggcatcggtc gcctgccggt agcgccctac gacgacgccg ggtcgcgacg ggtccaggca
    15541 atcgcgcacc gagcgtccat cgagaaggtc ctcgcccgcg atcacgagcg gcacctgcac
    15601 cgcggactcg ccgcagcgcg gcagccattt cgcgatgatg tcctcgcccc accggccgtt
    15661 ctgcgggagg gcgaagtcgg tatccggctc gttctggaaa tgctgccagc cgcgcgccac
    15721 cgcgggcgtc ggcagggggg gctcgttgcg gtcctggttg cgccgcggcg cgtggctcaa
    15781 gtgcgggatg gcggcgaagg acgagaggaa gccctgttcg agcctgtgcc actccgggct
    15841 gtccacctcg atcttgaacg cgtggcgcag gaagttctcc ggcccggtgt tctcgtcgag
    15901 gcgccggatc aggtagccga tcgcgttgat gaaattttcc ttgtggcagg ccggcgcgta
    15961 cagcagcaga ttgcgcgtga gctcgaagag ggcgcgtcgc tgcgcgttcg ccatgccttc
    16021 gagcatctcg aactgcacct tgtcgagcgc accgcgctcg tgcgcgagta ccaggccgta
    16081 ggagaggtcg aagaggttgt gcgacgcgat gcccacgtcc atggcggcga ggttttcggg
    16141 cttcatcagc tcgtcgacca tgcgcttgta gttcgcgtcg gtctcgggct tggtccggta
    16201 aggcgcctgc ggccagtcgc gcaccgacga ctcgcagcgc tcggcctcca tgttcgcacc
    16261 cttcaccagc cggatcgtga tgcgcccgcc gccggaggct gcgcgccggc gcgcccacgc
    16321 ctgcaactcg agcagcgtgc gatatgagtc ggggatgtac gcctgcagcg cgatgcccgc
    16381 gcgcacgtgc tcgaggcccg cgcgatcgag cgtgcgcatg aacgtctcgg cggtcagctc
    16441 cttgtcgcgg tattcctcca tgtcgaggta cacgaacttg ctgacctcgc gaccgtccgg
    16501 cctggtgaag cgcgcgcgat ccgccgtgcg gaacaggcgc tccaggcggt cgcacagcgt
    16561 ggcgaccgtg tgctcgcggg cgagcggcga gatctgcgag tacagcgtcg agatcttgat
    16621 cgagatgacc tcgatctccg gccactgcag cccctgcagg atctcctgca ggcggcgctc
    16681 cgcctcgacc tcgctcagga tggcctcgcc gaggaagttc acgttcatcc gcacgccttc
    16741 ggtgcgacgg tcggcaaggt ggcgcgtgag cacctccttc tcgcccggca ggatgacgtt
    16801 ggcggtctcc ttctgcatct gctccttgac cagcggcagg gcgacgccgg gcacgaagct
    16861 gccgaacgac tggaagccct tcatcagcgt gcggtcgacc ggcccgaaga agcggggcac
    16921 gccctgcacg tcgaggatgt gcgtcaggtg ctcgaccgcg cgagccggat cgttcgtccg
    16981 gaaagcctgg tcggtcatga gcgccagcgt cgccttgtcc gacggggttt ggagcatgcg
    17041 gtcgagctcg gcctgctgcc gccgctcggc cggggtctgc agtgagcggg cacgctcctg
    17101 caggtggcgg gccaccatga gcgccttctg gatcgttgcg gcgtggggtg aggacgggtt
    17161 cggcttgaat tggccgagca gcgtgtcgag cgtgactgca ttggtcatgt ggggagtccg
    17221 tcttgcgggc gggcttggcg cagggcgcat attctggcag ggatcgaggg gcggcagtga
    17281 atcacccccc tcccttgcgg cggggcgccg gcggcgatca ggtcccgcgc gtcacggagc
    17341 ttcgatcgcg ccgatgtccg cggccgactg ggtgggcctc gagtttccga gctggtcggt
    17401 cgtcagtcgc gagacgccga gtgcatcgcg ggtctggatc gccacggtga tcccctggtt
    17461 ttccgccagg cgcccggaag taaacagtgc ctgccacgcg gagtcgccca gcagttccgg
    17521 ccccatgccg gcgatccctg ctgcgccgag cgcggtgtcg agccggtgcc agaactcgat
    17581 gtacgggtag ttgtagagtt cacgcggcca ggtctgcgcc ggcccgaacc actgcgtgtc
    17641 cgccagcgtc aggatggccg taccgtgggg cgtccggtag ggctgcacgc cggccgtcgc
    17701 ggcgtcggag tccgccgtct gcaggaagct ctcgaattcg cccgtgaaac tcgccgcgaa
    17761 cccggcgagc ccgtagtggt gttcgatgcg cccgagcacg atcgcgagga agtcgtccgt
    17821 cgctccggcg ctgacgccgt actctgccag cgtttccgcg actcggtacg tgcccgccgg
    17881 cacctgggcc agggcgctgc cagcgggcga gtagtacaag acgacgggat tcccggcgtc
    17941 ggcgccggcg gacgggatcg tcgcgtcgac ggtcacaccg cccgccaggt ccacgacgcc
    18001 agccagcgcc acgccgctcg cgtcgccctg cttcgggaaa tgccgacggc acagcgaggc
    18061 ccagcctggc tcgccgaccg ggacgagaat ctggctgaaa tcgatcacgc cgaagcggtt
    18121 gtagccggcg ctgtggaagt acatcagcga gccggtgtag aggtcgtgct cgtaggtcgc
    18181 gccgccgatt tcctgcaccg tgttccctgc gacgatggac tggccgatgg tcatgccctc
    18241 gacggcgtgc gcgttgccga ccgtggcggc gatgccgccg gcgagattcc ctcgtccgag
    18301 actgtcggtg cgcgcgacgc cgcggacgac gttctcgacg atcgtggcgc ccttgacctc
    18361 gagactgccg ttcgacatgt agatgccgcc gccgcgccaa tagcccgtgc gcagcaggaa
    18421 tgccggcagc ccgggcgcag gctcggcgag gttgcgcgcg atggtggagt tcgtcacgcg
    18481 cagggtcttg ctcttgccga tcccgccgcc gtcggagtag gcgccggcgc cgtacgcata
    18541 cagcccgcgg atcgcgttgc cggtgatcgc ggaacggtcg atggtggata gtgactgcga
    18601 gctctccgct cccccgaccg agtacacgcc gccaccggcc gcgccgccgc cgagcaccga
    18661 gttgccgctc acgacgcagc cctgcatcgc gacgatgtcg gcatacacgg ccccgccgaa
    18721 ggcgccccgg tcgcgcgact ggtcgaagtc gccttcgacg ctgttgccgt gcaggcggca
    18781 gtccaccagg tgcgccgttc cccacaccgc gacggcgccg ccgcgcgcga gcgtccaggg
    18841 ctggtcgtcg ggcgaggtcg tcgggagctg ctcggcgacg ctgtgcccgc cggtgatcgc
    18901 gacgttcctg agagtaaggt ctccatacac cgcgaggacg cgggcgggag ccgtgccggt
    18961 ccacgccagc gtgatgccag ccgctagact cgatgcgtct ataacaacgt ccttgtgcgc
    19021 gtacagcgca gacctgccgt aatcgcggtc gaagtagccg acgaggtacg aaacgggccc
    19081 gctcggctcg ttacgcatcc ccatcacttc gcccttgagc ttcgtgtgct cgtccgcgac
    19141 gagcgacaac gcgatcgtgc cgccgctcag gctcgcgtcg aactggatgg cctgcccgga
    19201 ggcagcctgt gccagcgcgg atcgcagcgt gacggtgccc gcgggcggcg tctccatgtc
    19261 gaacaggctg ttgacgacga gcggcgtgcg ggccggggac ttcgagccgc caccgcctcc
    19321 gccaccgccg gagcaggcag ccacgcatgc gaggaccgtg gccacgacaa tgtgacggac
    19381 gctgggcgcc attgcgggac cctctggcca agtgcctgtt ctcatgctag tcgtgcgatt
    19441 cggctggtcg cgtacgtgaa atccgtggcc cgagtgccga ttcccgcgca gctcgcgcgg
    19501 ggcgcggtca tccgcccaat gcgtggtcga ggaaccgggc aatcctggca ttcgcctcgt
    19561 cggcgagcgg aatcggcagc gcattgaagc cgtggatgcc gccggggtag acggccagtt
    19621 cggcggcgtt gccgcccgcg gcccagcgac tggccatgaa cagcgaatcg tcgagcagcg
    19681 ggtcgagcgt gccgacgctg aacagcgccg gcggcaggcc gcgcaggtcc gcgtgcagcg
    19741 gcgacacgtc ggggtcgagc aggtcggaag gcgcgtacca ctcgaggaat ttccggatga
    19801 tcggcgtcga cagcacgagg ttgcgctcgc cccagctgcg ggcgctcggc gtcaggccga
    19861 gatcgaacgc gccgtacgtc aggtacgcgc cgcggaatgg actcaggccg tggcgatcgc
    19921 gcaggcgcag cagcgtcgtg accgacaggt gcgctccggc ggactcgccg ccgatcagca
    19981 gccgatccgt gccgaattca cgccgcgcat tccgcgccag ccacagcgcc gccgcttcgc
    20041 agtcgtcatt gcctgccgga tagggatgct ccggcgcaag ccggtatccc acgctcacga
    20101 ccgcgaggcg caggctgcga gcgagcgcat cgagcagcac gtcctgctgg tcgtgcgaac
    20161 ccagtgacca gccgccacca tggatgtgca gcagcacgct gcgtgcgtcg ggtgtcgcga
    20221 acactcgcaa cggcagcggg ccggccggtc cgtcgatggt ccggttcgtg ccgtttggcg
    20281 agagcacgat cgggccgaag aacgacttgc cctcggcgcg tgccgcgcgg acgacggacg
    20341 ccggcacctc caggatcgtc ggcagggtgc tcagcaggtg ctcgagcgcg gcattggccg
    20401 cgccggtggt cgtggcgatc cgggcgggat cgaacgcggc cgggtcgacg caggggccgt
    20461 ggatgtcact catgcggcga ttccatgcgt tgtgcgccgc gcacctgccg gcgtccgcgg
    20521 ggtcgctagg caccgcggct gccgcgcagc cgctcgaggg caagcatgcg gcggatggca
    20581 tcgagcgtga agtcctctcc cggcagttcg cgcagcacgg cccattcccg ccgcaggctg
    20641 gcggacagtt cctcgggcag gccccgcagc cagccttcga tgcgcaagtc gacgaactcc
    20701 cgcttctcct cgatggtgag ctcccgggcg agcagcaggc gcggcgcgtc ctcggaatcg
    20761 accatggact gcagcagggg ccagcgatcg cccggtgtct cgccgcacag cggggcgaac
    20821 gcgacgctcg acgtctcgcg gatcagttcc tcctcgcggg tggtgcggct ctcgtgctcc
    20881 ttggtctcgt cccagcattc ggaatcctcg gccatgtagc gcaggtactg catcgcgaac
    20941 aggcaggtcg gcgcgaccag atcctgccgc ggccgggcgt cgatccacag cgcggcggcc
    21001 gcgcgcaagc agcgccgcgc ggtcgtctcc gccgccttgc ggccgaagac cagggcgacc
    21061 gagtccttga cgaaggcagg cagttcggtc gcgatgatct cgcgatatcc ggggacgtgg
    21121 cgttcgagca gcacggccgt gcgacgatcg agcaccgcca gatcgctggt cacggcgggc
    21181 aggccgagcg tggtgcaggc gaggttgtag acgatcgcgc gcggcagcgt gacgccctcc
    21241 ggccacgcgt cgcgcagatc gcgatcgtcc ggcaggcgca ggtggccggt ccactcggcc
    21301 gggtagctgg ggatcgaccg gccgtaattg accgagccgg ttgcgcggat gcgcacgcgc
    21361 gtctgcggca cgaggctgcc gaacggggcg agcagcggcg cagtccgcag cagcgtctcg
    21421 gtgtgtgcct gcacgccacc ttcgagcacc ttgcgcagcg cctcggtgtc ggcgcgcgtg
    21481 atcgcgtgcg cgggctcgcg cgtgccggcg gcgcacgcgg cgcggaaggc cgcagccagg
    21541 cgcgcggcgt cgtccggcgc gtcggcacgt atgccctgca gcgtgacgcc ctcggcgcgg
    21601 gcgaactggc ggatgacccc tggatgttgc ggatcgaagc cgccccggaa gccggcgcgg
    21661 ccagcaccga cgagcagggt cacgcgtggt gcgggcagac cacgcgatgc ggcagcggcc
    21721 gcgaggcgct gctccgcttc ggcgaccgag ccgacggcga gcgcgtaggc cgagtcggtg
    21781 gcgagcttgc cgctctgcag cgccgtgtcc gacatggcga cgaacacgcg cacgacggcc
    21841 tcctcgcgct cgaacgatgg gctcagcatc gccggtgtcg gggagccgtc gaccccgacg
    21901 ccgcgtccga gcgcgacgta ataggcctcg agcaggtcgg gcaggtgggc cagtgccccg
    21961 acgttctcgc acagtgggac cagccgcacg cgccggaggc gctcgaggat cgagcgacgc
    22021 cggtccggcc cgacgcgccg taccaggaac gcgtcgtgct ggtcgctcgc agggtcgagc
    22081 agcgcggtcg cggcctggag gtacagcgtg ccgaccttga gcagcgcctc gatctcggcg
    22141 cgcgaattga tctgcgggac ggtgatctcg tggatcgcat tgcggacggg gacgacgccg
    22201 agccgttcgc acgcgagatg gaacagcacg ttggcccttg cgaccgacgc gaggatcctg
    22261 gagaccttct cctcgtcctg ctcgaggaac gggttcggcg cgcgcagcag caggtgccgg
    22321 tcctcgcctg cgacgacgcc acgtcgcagc agctccagta cgaccttctc cggcacggcg
    22381 agccagtccg ggctgatctg ccggccttcc atgtccagca gcacctcgct gcagggaccg
    22441 agcaggtccg cgatcgattc cgcgtactcg cggcgtgcgt ccatcagcgg attccactcg
    22501 aggtgggtca gtgcggcgtg ggcgtggcgg tccgcgtgtc cgccagccgc ctccagggcg
    22561 cgctcgaact gctcgacgcg atgggcggtc gagtcggggt gcttggtgcc ggtgagccgc
    22621 aacgactcgt ggcgtggatc cgccaggaaa cggcgacgcg cttccgcgaa gccgaccggg
    22681 tcgatcgcat ccagggcgtc gtagtgcccg gccgtgcagg ggctgtccca ggtcaggtcg
    22741 tgaggcgacc ccgcggggcc gaccgcggcc gccgcggctc caagccgccc ctgctgctcg
    22801 atgcgcgccc gcacccggtc catgaagacc gcgagttcct tgacgtcctt ggagccgtac
    22861 gaaagacgct gccaggttcc gtagcggtcg agggcgccgg ttccgccggc gaacgcggcg
    22921 aacggaatgc cgcctacgcc gccggacagc aggaactcga aggcgtcgat ggaattgcgc
    22981 acgacgacct gctcctgcac gccgttgcgc acccgctcga acttctctcc caccaggccc
    23041 gggaaggcca gcggcgcgta gaaggtgccg ccgaagtcgg tgcgctcgat gccgaggtgc
    23101 gcgaggcggt cggcggcgcc gatcatgaag tcgcgtcgtt cgcgcatgta gggcgacggt
    23161 cggcgcagtg ccgcgagcgg gctctgcagg gcggccagcg cccagtcctg cgccagcgtc
    23221 gacggcgtgg ccaccagcca cgccgacagg ttggccagga cgtcgcgtgc gcggatgccg
    23281 ttcgcgcgga tctggtcgga gagcaccagc cagcacgcgc gcgcgcccgt cgacgcgccg
    23341 aacatgtcca tcttggacag ccccgacagc gacaccacgt ggtcgcgcca ctcggggttc
    23401 gcgaacgccg ggttgacgaa ccagccgacg tagtcgaggc gctggtagac gcagtcgtac
    23461 agcacgaaac agcggtgacg caccgccacg cgcacgatct cctcgaccag gctgcgcggg
    23521 tacacgcgcc cggtgggatt gtgcggattg ttgaggatca ccgaggagac gccgccccgg
    23581 gccaggaggc gctcgaggtc cgccgggtcc ggcaggaact ccggggcgct ggtcggcaac
    23641 tcgacgacct tcttgccggc gcgctcgatg aaccagtcgt actcccagct cggccggggc
    23701 accacgacgc cggagccgtc cgcaccgagc acttcgagca cgatcgcaag accggcgcgg
    23761 gtgcccggcg tgaacagcac ctcgtcgggc atgaagcgca cttcggtgcc cgggtagtcg
    23821 acgagcccgt cgcggttgaa gaattccgcc acctcgctgc gcacgtcgag ccggccgacc
    23881 gccggtccgt agctgtgcac ggtgttgcgg gtgatgcgct gcgccgcccg gacgacctcg
    23941 ggatgcggcg cgaagtcgga gttcttgtaa tcgccctggg tcaggtcgag ggcgccttcc
    24001 tgctgcacgc ggcgtgcgaa caggcgcacg aacggttccg gcgtgcgcag ggcgtgggcg
    24061 gaaatggcgg cagtcgggag tgcgaggtct gcgttcatcg tcggggccgg cggtggttgg
    24121 ttgccggcag acgattcttt cagcaccggc gcccgcggct acacgcactt gccgcagtcg
    24181 cctcggcccg gcacgcccta tcttggtgcg gcagcggccg ccggagcggg ctcggccgtt
    24241 tcgagcaacc ttgcttcgtc gtcggtgaac agtcgcgagc gcgtgaggaa gcgccggccc
    24301 gtcggaccct ccagcgagaa catgccgccg cgaccaggca ccacgtcgat cgtgaggtgc
    24361 gtgtgccgcc agtactcgaa ctgcgcgcgg ctcatgtaga acgggcagcc gccgatctcg
    24421 ccgagcagga cgtcggagtt gccgacgagg aattcgccgc gcggatagca catcggcgag
    24481 ctgccgtcgc agcagccgcc cgactggtgg aacatgaggt cgccgtgctg cccgcgcagc
    24541 acggcgatca gttccagcgc ctccggcgtg gccgagacgc gctccaccat cagaagaagc
    24601 ccagtgcctt cgggctgtag ctcaccagca ggttcttggt ctgctggtag tggtcgagca
    24661 tcatccggtg cgtctcgcgg ccgatgcccg actgcttgta gccaccgaac gccgcgtgcg
    24721 ccgggtacag gtggtagcag ttggtccaca cgcgaccggc cttgatgccc cggcccatgc
    24781 ggtacgcacg gctgccgtcg cggctccaga cgcccgcgcc gagaccgtag agcgtgtcgt
    24841 tcgcgatctt gagcgcgtcg gcctcgtcct tgaacgtggt caccgagacc accgggccga
    24901 agatctcctc ctggaagatg cgcatgcggt tgtgtccgcg gaacacggtc ggctcgatgt
    24961 agtagccgct cgcgatgtcg ctgcccacca ccgccttgcc gccgcccgtc agcacctggg
    25021 cgccctcctt gcggccgatg tcgaggtacg acaggatctt ctccatctgt tcgctcgagg
    25081 cctgcgcacc gaccatcgtg tcggtctcga gcgggtggcc ctgcttgatc ttcttcacgc
    25141 gcgcgacgac gcgttcgagg aacttgtcgg cgatgctctc ctgcaccagg gcccggctcg
    25201 ggcaggtgca cacctcaccc tggttcagcg cgaacagcgt gaatccctcg aggctcttgt
    25261 cgaggaaatc gtcgtcctcg gccatcacgt cctcgaagaa gatgttcggg ctcttgccgc
    25321 cgagctcgag cgtgaccggg atcaggttct ggctcgcgta ctgcatgatc agccggccgg
    25381 tcgtcgtctc gccggtgaag gcgatcttgg cgatgcggct gctcgaggcg agcggcttgc
    25441 cggcctcgac gccgaagccg ttgacgatgt tgagcacgcc cggcggcaac aggtccgcga
    25501 tcagttccgc gacgagcagg atgccgaccg gcgtctgctc ggcgggcttc aggacgacgc
    25561 aattgccggc cgcgagggcc ggggcaagct tccacgtcgc catcaggatc gggaaattcc
    25621 acgggatgat ctgcccgacc acgccgagcg gctcgtggaa gtgatacgcg accgtcgact
    25681 cgtcgatctc ggcgagggtg ccttcctgcg cccgcacgca ggccgcgaag taacggaagt
    25741 gatcgacggc gagcgggatg tccgcggcca atgtctcgcg caccggcttg ccgttgtccc
    25801 aggtctcggc gatcgcgatc atctcgaggt tggcctcgat gcggtcagcg atcttgttga
    25861 ggatcgcggc acgttgcgcg accggcgtgc tgccccacgc gtcggcagcc gcgtgcgcgg
    25921 catcgagcgc gaggttgata tcctcctcgg tggaccgggg cacctggcag aacaccttgc
    25981 cgttgaccgg cgaaacgttg tcgaagtact ggccgcgcac cggcgcgacc cacttgccgc
    26041 cgatgaagtt ctcgtagcgc gacttgaagc tgaccttggc gccggccgtg cctggatcga
    26101 ggtaacgcat gaggtgtctc ccgcctgttc acgaagaaaa agaaacccct cggccccgag
    26161 cggtttcgcc gacgcatggt aactccaacc gcgcgccgga acgatgctgc gccgcgtcat
    26221 gccggcgggc ggcggcgtcc tcgcggccgg tatcgccgcg gacaggctag ggcagcgtct
    26281 gcccgcaggc ttcgcagcgc cgctcgggca ccacgtgcgg ctcgtgcagg cgcaccggcg
    26341 caccgcgccg ccgcagccgg atgttgagca gctcgacgag cgtcgcgaaa gccatggaac
    26401 cgtagatgta gcccttcggg atgtggaagc cggcgccgtc cgcgaccagg gccacgccga
    26461 tcagcagcag gaatgccagt gcgagcatct tgatcgtcgg atggcgattc acgaatccgt
    26521 gcacggactc ggcgaacacc agcatgaaga tcacggcggt caggatcgcg gcgaccatga
    26581 tctcgatctc atcgaccatc ccgaccgcgg tgatgatgga gtcgagggaa aacacgacgt
    26641 cgatcaccat gatctggccg atcacggcgg cgaaggcggt cgacgcggca gccgccgggg
    26701 cggaaggagc cgccgctccc tccagcttct cgtggatttc gcgggtcgcc ttgtagagca
    26761 ggaaggcgcc gccgacgatc agcacgaggt cccggccgga gatgtccgtg gcggcgaccg
    26821 tgaacagcgg ctccgtcagc ttcatgatcc aggcgagggc ggcgagcagc atgagtcgtg
    26881 agaccagggc ggccgcaatt cccatcctgc gtgcccgggc ctgctgctcg tgcggcaact
    26941 tgcccgccag gatcgcgatg aagatcaggt tgtcgatgcc gagcacgatc tcgagcgcgg
    27001 cgagcgtagc gaaggcgagc caggcctgtg ggtccagcag cagttcgagc atgaaggtct
    27061 cccgaaggga gtccagattc taaggcacgg cgtcgcgttg cgccgctggc gcgtcctgcg
    27121 gcaccaggag gcgcccgacg gcacgcagcc ttgcgaaacc gcgcacttcc cctggaaaca
    27181 gcggcacttc gacgatcggc acgctcccgt agcgatcgcg aattcgcagc aggtgtgact
    27241 gctgctcgcg ccgcttctgc tgcaggaatg ccgacggcgc atcctgaacg acgttgttga
    27301 tcacgaggcg tgacatgcgc aggccgtaac gggcgagttc cccgaccgca tcttcgagct
    27361 gatacaccga cagggcctcg gggatcgcca ccatggtgaa tgcgacgtcg cggcgcagga
    27421 actccatgca ctctgcggcc aggtcgcgcc agcgctgcag gatcgcgatg accggctccc
    27481 tgcgctcacc cgtcgtgcgc aggtgcgagt agatgcgggg cgcggcgcgc aggtgctccg
    27541 cgagcaacgc aggcatcccg agcagcgcga gcgtctggcc gagcggcgcc gtgtcccaca
    27601 ccaggtgccg gaagcgtgac tcgatcgcga ggcgacggat gtaatcgacc atgaactcct
    27661 cgttcaggcc cggcagcacg gcggtgacga actcgacgaa ggtcgtatac tcgacatcca
    27721 cgaaactcga gaacacgccg tagacgtcgc ggccgaaccg ctcgtcccac aggttgcgga
    27781 cgtccttgag gccgagttcc gtgaggtaca accgcgggag cagttcccgc tcgcgatgac
    27841 cacccgcgat ctcgaagatg tgcgacagcg acggcgtcgg gtcggtggaa atcaccaggc
    27901 tcgaatcgcc ccgctcggcg agcgcgagcg cagtcgccgc cgagcaagtc gtcttgccga
    27961 cgccgccctt gccgatgaag acctgcacac gggttgcgtc catgccccat gattccaccg
    28021 ttggcgaacc ccgcctatcc gtgatcgccc ggcccgtcat gcaccgatcg cgaaatggcc
    28081 cacggagtcg accccgacgc agggcggcat taccctgatg gcccgcggga acgggccccg
    28141 gacacgaccc gggcaacgcc gcgaggggag agcagaccat gagcgttcga tgcaatcggc
    28201 gtgccttctg ccaggcgatc ggcgcagccg ctgcgatcgc cgccatgcgg tccgcgcgca
    28261 gccagccgga ggaatccgtc atgctcgagc gccccattcc atccaccggc gaacgactcc
    28321 cggtcatcgg cctcggaacg tcggacgtct tcgacgttgg accggacccg gccgcgcgca
    28381 aacccctgca tgaggttctc gacatcctcc tcgggtccgg cggctcgctg ctcgacacgt
    28441 cgcccatgta tgggcgcgcg gagcagg
//
LOCUS       DAOMFD010000006        27232 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_261_length_27232_cov_31.786290, whole genome shotgun sequence.
ACCESSION   DAOMFD010000006 DAOMFD010000000
VERSION     DAOMFD010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 27232)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27232)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27232
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_261_length_27232_cov_31.786290"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1141
                     /gene="guaA"
                     /locus_tag="PL152_00795"
     CDS             <1..1141
                     /gene="guaA"
                     /locus_tag="PL152_00795"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQR47837.1"
                     /translation="FKLMASTASCPIAAMADETRGYYAVQFHPEVTHTRQGRAILERF
                     VLGIAGARPDWIMKDHIAEAVAAIRAQVGGEQVILGLSGGVDSSVAAALIHRAIGDQL
                     TCVFVDHGLLRLNEAQMVMEMFAGRLHAKVVHVDASGQFLGHLKGVADPEQKRKIIGR
                     EFVEVFQAEAGKIEGAKWLAQGTIYPDVIESGGAKTKKATTIKSHHNVGGLPETLGLK
                     LLEPLRDLFKDEVRELGVALGLPHDMVYRHPFPGPGLGVRILGEVKREYADLLRRADA
                     IFIDELRGTVDPATGRSWYDLTSQAFAVFLPVKSVGVMGDGRTYDHVVALRAVHTSDF
                     MTADWAELPYSLLKKVSSRIINEVRGINRVTYDVSSKPPATIEWE"
     gene            1439..2698
                     /locus_tag="PL152_00800"
     CDS             1439..2698
                     /locus_tag="PL152_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005413108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HQR47838.1"
                     /translation="MLSDGTLRGIKPQATTYKIADRDGMYVTVSPRGTITFRLDYRLN
                     GRRETLTIGRYGSKDGISLLMARERCVEARKAIAEGRSPSQEKQREKLRTAEAKTFEE
                     FTKRWLEEARMAESTKSMRKSIIDRDIMPVFRNRLMAEIGAEDLRNLCAKVKARGAPA
                     TAVHVRDIVKQVFGFAALHGEKAPNPADEVGPSSIATFVAKDRALSPSEIRVMHRVLD
                     ATATLPTIRLALRLILLTLVRKSELIEATWDEVDFENAVWTIPKSRMKAGKPHNVYLS
                     QQALDIMVALRTCASGSKFLLPSRYDADRCMSKATLNRVTQTVAERAKEAKLPLEPFT
                     VHDLRRTGSTILNELGFNSDWIEKCLAHEDGRSSRGVYNKAEYAEQRRHMLQEWADMI
                     DAWVAGKTHTPTLLPPTMKVATTAAPI"
     gene            2750..3475
                     /locus_tag="PL152_00805"
     CDS             2750..3475
                     /locus_tag="PL152_00805"
                     /inference="COORDINATES: protein motif:HMM:NF016163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HQR47839.1"
                     /translation="MDGCRAGWIAVADVGGGLTYRLHASFAELLASWSTAQRILVDIP
                     IGLPWRDCPSRPCDRQARALLGPRASSVFSPPSRRASRAANVGQARAWNLDEVGRSLS
                     AQAWGICKKIVEVDRVLLEDTSANRKVFEVHPEVCFWGLNGGKPMRHRKSNPEGVRER
                     LAALSVRLPAADHLLQRVLRETRRQDVLADDVLDALVAHVTGRAVEAELRRLVGVPSE
                     DDEGLPMQMLFCEYELTPLPPGS"
     gene            complement(3554..4003)
                     /locus_tag="PL152_00810"
     CDS             complement(3554..4003)
                     /locus_tag="PL152_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47840.1"
                     /translation="MGQLKAMDEKREFPPALRIPAANLRKLLQREQHDAANEFRTLKE
                     LKSPNTWVAVPAGHYQFMFSETAEDGKPFRLDDQPLWQEALGRTLNAGSAVMPPLPKY
                     ESFPWAATVGEVSPLKLDLVFDDRYFMASNELNLLNTLLLSSSEGVD"
     gene            4006..5400
                     /gene="traD"
                     /locus_tag="PL152_00815"
                     /pseudo
     CDS             4006..5400
                     /gene="traD"
                     /locus_tag="PL152_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
     gene            5492..6205
                     /locus_tag="PL152_00820"
     CDS             5492..6205
                     /locus_tag="PL152_00820"
                     /inference="COORDINATES: protein motif:HMM:NF025706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HQR47841.1"
                     /translation="MSSRNAAFHDGALAGVLLSPLKLAFSLALGLVALLLCAWIVDWV
                     FVFKVWPQGIERLRELLTHDLASGIALAARQGADAGVITAPANALYSIVFEATGIHDM
                     GTQFANGSALSIPDTIMRRSYVSHREGIEAAMVGTQLLGVRAAILARFAPLLLLLYAV
                     GAADGFTQRAIRRACGGRESASLYHRAKYLQLAVLGLGGVSLLLWPGPVQWERCVALG
                     ALLAGGLASVQWAYYKKHM"
     gene            complement(6244..7434)
                     /locus_tag="PL152_00825"
     CDS             complement(6244..7434)
                     /locus_tag="PL152_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47842.1"
                     /translation="MRRQGTPVSREALYEEVWTDAVTVVAPRYGLSDVGLVKICKKLG
                     IPVPPRGYWAKVKAGRPTRKVPLPALPAGARDLAGPIPLSEQEAAMHARVRDALQQTR
                     ESQSPVSVPAELVDPHPLVRATAARLKRRDGWDHPAGVRSAPKEVLDLQVTRNTLDRA
                     LLLMDTLLKSLEPSGFTARVDEEKGETLLVGGGTTLTISIVEQVTRTSHTPTRAEVRA
                     RDRYYDSFRVGARGEYPNIPQFDWHPTGRLTLTVGSWPSRKWNDTERSLIDSRLSGIV
                     AAIVGLAEAKRAKEEEEERRRRTYEEARARYEAQVRARNEERRQLRALFRDASRLQRA
                     NRLREFIAAVEDRARHDDELTPAKQQWIEWAKAKADWLDPLVRRSDPILDAPEPEAPS
                     FWHF"
     gene            7580..8659
                     /locus_tag="PL152_00830"
     CDS             7580..8659
                     /locus_tag="PL152_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47843.1"
                     /translation="MLHAQASERELIDQFLAALRSLPEVQAELERVGSPELDAQLALD
                     VAGKPIHALVELRKAVYPRDVRQLVWRIRDLARQQPAGESGSEPLAILVAESISPGAK
                     ELLRAERVGYYDSGGSLYLPARGAYLYVDRPPPKSLSRSMRSLFTGRRAQVLHGLLVR
                     HQDWFGVKDLAEQTLVSSATASQVLTEMERFDWLESRGQGPSKQRHLREPAALLDAWA
                     KQLSSIRPPAMRRYFVPAMKPDGLMERLGQVCEAHSVDYAVSFEAAAQRYAPFLSAVS
                     QVRCRLLAGSGGDAAIADLGARVVSEGANLAVIEAKSDGDLLFRERVGGVWLASPVQV
                     YLDLLRGEGRAKEMAEHLRRERIGF"
     gene            8659..9555
                     /locus_tag="PL152_00835"
     CDS             8659..9555
                     /locus_tag="PL152_00835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HQR47844.1"
                     /translation="MAKPATFDGYSDQYTLDCERVLVTLLRGLGPWKDSVYLVGGLTP
                     RYLVPARPPVVPAHAGTLDVDIVIDLQILADTEAYHTLEENLKRMGFERAENEQGAKL
                     SWRWQTRTEHGALMVLELLADAPQIAGGRVQPLPTEGTISALNIPHSSIVFDLYQVAE
                     IRAELLGENGMATERVKHADIVSFTCLKAFAFDQRFERKDAHDLVYCIEHAPEGLDAV
                     VGAFTRALAGKHAAVVREALDILRRRFADEEATEGYRKDGPVAVAKFELGEGDEADQR
                     EARVLRQRQASDVIDRLLAGIG"
     gene            9572..12202
                     /locus_tag="PL152_00840"
     CDS             9572..12202
                     /locus_tag="PL152_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQR47845.1"
                     /translation="MLITFGLRLDGERGWRPANQLGRPVFGPLGLLNLLETRLGLLRS
                     DCAHAQRVTQYRECLKQFDTPDRFYHASFGIDPIGTSASLLSWRDTWHLHGWNGQSPP
                     AAGVRMTDMAAVESAARELLFPSIGERLARVAEFLARQQSSIDGIELVDPLDAFPKRW
                     REVLAKLPVRSIRAYGPSADPITVLGRLQIALKAAHKGEKPEGKIAWLDDGSLRVVRA
                     ETGLVAARCVADDLAAPQEGIAIIAEQSRSLLDATLDGSDVARQGFLDASPLAPALQV
                     LPLALATVWEPLDVYALLQFLSHPIGPVPGYARRRLAEVVAQFPGVGGPRWREAVEEI
                     VRRHPERADDLRRALAFWVEHKRFDPKQGAPTSALLERTRAIRDYFGARLADQDANRS
                     AAAATGLAQAAAVTAALEALAAQGEASVAPVELEALVAQCTARGAPNFAMHAQVGCAP
                     AATDPGALLEPFDRVIWWQMGAPSLPGHYPWSASEMASLSRAGVELPPLEELLTRRGE
                     EWLRPVLHARRQLVLVLPPYGEEMHPLWQEIQWFVDGVRPEPLEGLLTGRAGNELPTI
                     AHAALPARRRWWDLAAQATIPPRTRESYSSLSAFLNAPHQWVLKYAARLNPSNLLAVA
                     DANLLYGNLAHRLIDRFFRTSGSLALRGEALRAWFSREFETVVAEEGAVLLMPGRRGD
                     YERLRSSLERAIAEFQRQFAAAGVDAVEPERALAGSFTGGELEGVADLVVRNRSGQRA
                     IVDMKWSGGNYHQERLAKNRHLQLAVYAELLRQETGAWPQVSYFILEAARLIAPDTGF
                     FPEARAVQQGGPGSTAVLWDRFVAAWNWRRSQIDAGLIEVAARGIEPTAESVGPAEAL
                     EPEELREDYDDYRWLTGWEG"
     gene            12206..15352
                     /locus_tag="PL152_00845"
     CDS             12206..15352
                     /locus_tag="PL152_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQR47846.1"
                     /translation="MAGNLRFVSAGAGSGKTYRLTQILHEKLSSGQARPGGVIATTFT
                     RKAATELRERVRTALIEKGEFALANSIGQARIGTVNSVCGGYLERFAFEAGLATEQRV
                     LEEAQSGALVREAIDRVSDSASVQELVELARRLGIESWEDALKSIIDQARANDIAHTR
                     LAAFGERNARELLAHFPAPATDDLDALLVSAIEAAVPELEKRATGKKNTADFIALARE
                     TLRLVRGGTATWSDWVKLSKSAPEAGLRTVAQPATDIAGRFPAHPRLRQDIERYLAAI
                     FALGARALDAYAQRKRELGVIDFVDQEHLFLRLLEKPAVVEALSEELDLLLVDEFQDT
                     SPIQLELFVRLSRIARESVWVGDVKQAIYGFRGSDAELMKAVLAELPAMGGTKEVLGQ
                     SRRSRPGLVRLVNGAFGDAFAPGLSRQEVELAPVRKELVPDAALANWVLNGRNVTERA
                     AALAEGVKALVASGYRVVDPSSGNPRPAHFGDIAVLCKTNVGVQTVAEALRIASVPWA
                     TQQSGLLSTPEAVLAVACLRRFNDPRDTLASAEIVSLADCEEPESWLADRLRYLEAGG
                     DSAQWRDGTEGGHPLLARIAKLREQAPLLSPYAAMELVVVQCDLAGRVLRWRRNELVA
                     QVRLANLQALLGLARTYEDACRARREPGTFSGLILWLGEQAGAQLDMLAQPPVDAVKI
                     MTHHAAKGLEWPIVVLLDLEKDIQDRIWSVGARSEGRLDVAAPLKDRWIRYWPWPFSA
                     QKKVDIADTIAQSAEGTRLRAEAMEEAKRLLYVSATRARDCLVLAFPAKRGACEWLGS
                     LNAPWLAPDVADDAVSLPDGSRVSCDFRSFDAPAAPSVAVEPDAPLHWFRGTESRTER
                     LPATLVASATQARACRITDSASIGDRIPLSAGIDMTALGDAIHACIATAFTDPSVPFD
                     EPRAARILGGFGLAGALEPSALVRQIAALDAWISARWPDCRRHAEIPIESILPSGQVM
                     HGRIDLLLETKTGWVLLDHKANPAPRDRWDQVAQEHGGQLSVYADALVRATGRSVAEA
                     WIVLPVGAGAIQVTASEL"
     gene            15429..16655
                     /locus_tag="PL152_00850"
     CDS             15429..16655
                     /locus_tag="PL152_00850"
                     /inference="COORDINATES: protein motif:HMM:NF039739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6361 family protein"
                     /protein_id="HQR47847.1"
                     /translation="MRHDAVTWLDFRADDLARARDFIRSLQEEGVIDELGFLALLGRF
                     SDLLHPATTTLMRSARYFYFIAGIYRSLEREGARASQIPHLSRQRQDELRDVLSDTET
                     IGVIGRDAGINLRQFPSQIYWSGLKKLGMFTSNLSESAYQEQFDDIRRSRRGYQDDDK
                     TPQGPEGVRFWSDDLPPASFLDSDGRIRPGATFKLTRAEASDLHKRFTSRFSESLLSH
                     MLKHRLIGIDWPWECPKLPESLAVWVWHARNMSLLVRGATLQYYSLLMEERDKLRIEE
                     DTDLVAPIFNEWWIAAKEPLKKWRTSEFVTVPTVASALRLGNRGDRWFIDGWLECCVA
                     ASTAEALLRDEKARHLIRERERRVKPAKARLKYEKHLRQWKPDRVGEGIYQFDYRHSI
                     GSRFVSEILDGMERGQ"
     gene            16961..18442
                     /locus_tag="PL152_00855"
     CDS             16961..18442
                     /locus_tag="PL152_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HQR47848.1"
                     /translation="MDGGAFHPKVWVLRFDPVARPERHSVEPVYRLLTASRNVTDSGC
                     WELGALFEGSRRTGTQKFGSDVAAFCRKVAASRDLPKALWKLIEELKYVEFVAPREAA
                     QGLRFDWQSPGDRVLINRLPRAMSRVLVISPFLRADFLKRLCDRTDDLTLVSTQDELD
                     QLSDETHERLANARVFVVSAEADEEAPSLDLHAKLLAWESDGVSETLIGSANATGPGW
                     GCGPNVNCEAMVSLNPGLGIDAVVKAFVAPAKDQLQPWIDRYVRQTAVADPEREAAKR
                     LEHFKRLLRADQIRGEYDPVKKTLRLLAPSSAGEQTWPADMRAEIVPLLQRDQSARAD
                     YRLVYGPGAAFQGIELEDVAAFAYLVVWSEHHRDVELAVVVQFELNLGALEADDRDKA
                     VNARLLEGVDARSLLLDVLAGLPGGTSRPAGGGQSGVWQAGDPLLRRATIERILEACT
                     ADPSRIHEVEAVLAACGDASNMSTFREFWAVFRESLEEEGAGV"
     gene            18435..21863
                     /locus_tag="PL152_00860"
     CDS             18435..21863
                     /locus_tag="PL152_00860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47849.1"
                     /translation="MSSLELAEFQRAAVETICERLTNAGGSRRFLLADEVGLGKTVVA
                     RGVIDELLRRRRGRELVVVYLCSNTEIADQNRTKLAPNAPPSLRRITQLAWSTKAGDN
                     LQLYSFTPGTSLSEGTGLAWERQLLLFLVHRVLRQDIRKGKWREYFRCGAGEERWKAA
                     TKFAVLRLEFNRKLRPGLQQRIAEEWRKPTEVDGTTLVLSEVLRGEVDQFKPTDAGSR
                     RRRNLIVGMLRSAAQRVLLDDLQPHLVILDEVQRFREVIEEATSRRSIAARLFERRPG
                     VLILSATPYRMLALDHEGQGHYDEFLDTVRFLFADQGKQEVARLRSDLKAFRERLELG
                     AFLRGHDHELLALRARIEQRLRKVICRTERNAYIHDPTKGVQEVRPTGNVFAVPQKEE
                     VVDYIRLRRFLLDKVDTSQHITEYWKSCPAPFTFMDAQYAPMAAAKRKKAVVPAGVIE
                     QASKLGALPRRNLRFRELFQAMFGGPDERWRFLWTRPTYTYYRDEFFREADPKKMLVF
                     SGWRFVPKAIALLTSHEAEQRIAPRGRLWEGDDRPPLRFTEKGSFHIFDVCLPSPALA
                     RLVEPSALASDALTAKELLRRTRKALKRALEEAGVEVAATSRSPIWQAVARLERHSDS
                     SIRDALEGSAVYNGDDITERFAEHVDTFVDWMEEEGSLRISEERLTHLARIAAFSPAI
                     SLLRAFWTTYPESRGEVHERLVDLCFGELRSYFNRRTVRAIVERSVPAGRGYARAAIE
                     YCERAHFQAIADEYLYLGKNVLQRNGPAEMAEHLARVLGVGTGSPNINLTTDTGRIRA
                     EQLVRRSHFALAFGEDVKVDQGVPLEQTGPSRKTAVREAFNSPFWPFVLSTTSVGQEG
                     LDFHLFCRDIVHWNLPSNPVDLEQREGRINRRDGLAIRRAIAREWPLERLAQAGPGSS
                     PNFWERVFSVVGEAPDIQRYKHGLYPHWVFEPRGGQVERIRRHLFFYTDSQDASRYEE
                     LKERLALYRLVFGQPRQQDLLERIQRTVRDEAGREVSHTALTRYMVNLSPLGEEHAQV
                     RAALEAQEIAASPDKLQQLLRDVEQIEAERAAELLPARGDLDRFKAMVRRHIESPYED
                     HGKLVTAIRVLTYLRDPYDAIMDQYVDLGLQDDVALIRSASRSLASCGTVGAKG"
     gene            22074..26978
                     /locus_tag="PL152_00865"
     CDS             22074..26978
                     /locus_tag="PL152_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024486.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HQR47850.1"
                     /translation="MEIRFLRKSGVPAAEISAHQRIQKVFDASPFTKSWRGYASFQLA
                     RGGRGSGGDDFDLVLVTHTCIVVIELKNWHGNTLKSEGGRWYVDGEDRGASPVDLVSE
                     KAKKLASRMKQKLGVEKTPFVNSFVVIQDGIENLELRDEEESRSVLFLTDDLLEWTKR
                     DAYEAVLPWRPRFNVLANLPLYDQFFGGTDFKPKDYLVQGYRPGDNPIWEHPRRLYSE
                     FRARAKDNPDQFALLRQWDFSAMGPALIGEGDRAFIGLREQRIYEYVAERNEELSLGL
                     LRPIGRHGDKDVNLDFAELYALPSKLVRLNEFSNSVLPRLTADERVLLVKALLNRFAE
                     LHELSVAHRDVAEHCLWLERPAKVVMTGFPAAHYPSMQTVGTFREQLKVERANLPEDT
                     GLAKEGTPYTRDVYLLGVQAHLLLFGERPPKLRDVYEWTPRADKDFGGAFDAVLAKAL
                     DREPSKRYSNAREMLEAVNAATSSPAQQAIDLSRFEAYRAASKQRDYEEAEVLTDEDD
                     LLFYRSKGAEGDLAVKVWIGVEPDARKPELSLRLLAFLERARVLKGCVLPGLPRIIDF
                     GLSRRSLLLVYEWVDGLALKDWLAKDPPLEQRLAIASMLVDTLARIHALELAHGDVHP
                     GNVIVAPTGQAFFIDAFDFRRGTEDAYTTAYLPPTYKSLTPMERDRYGVAAVIAEVLG
                     GTKETPEGPFPVPAVYEQLRKLLTDLQASALEPLQNALAKAAQAGEKATPAFTATVQY
                     LSRKGVPAGAMHPDNGIFHVSVERSKKRAGDAPLWFVRITGVGRQVTFDWNIEQGVAQ
                     NVWAGPITQAQLMWAQIYRDTTLEARIELADGAVADVDEVARAVLDLPEVSRKVGEAE
                     PAKQAPLAPSEASTTSAQDIGASAPTPATTLPAPIPELWQALLDAEEEVLPMVTVSGE
                     RRANPERRGQVLVPYHLDSGGIDYDGGDTVVVEFLGTDKNWRSCGLLSLRDTTFGDQA
                     ELAIDDFNSRTNLKIGERLRLRSTLEKASFTRRSQAVQRLLNDKAVIPGLTSYFNPAV
                     SAERVPLSYPVPTDADLDVYADGDKQLNESQRDAFRKVLGNGPISLLQGPPGTGKTWF
                     IASLLHYLMTKQQSRRILLVSQSHEAVNNALEKALDLCRDKGIPFDAVRLGNESAASD
                     RIRHLHSGSIEQAYRERFKAEQRLRITRLAVEMGLPEDFAREFVELHHRLGGLATRIG
                     FMAGEVESAPEGAERSAAMARLKSLLGSFDDICQDVYDESVDGPPATVIATLEARLIA
                     EHGVASPDAVHRLRRLVRLSEDWLSALGSPQANFSEFLAKSRTVVAGTLVGVGHRASG
                     VVQNLYDWVIIDEAGRAAPSELAVAMQTGHRILLVGDHKQLPPTFSQEVREAIKARYA
                     VSDESSVFGSDFERIFESDYGRRVGASLLRQYRMAPNIGELVSSCFYDGRLETGRGDP
                     PEFYSHLPNLLRHQVNWVDTTPLGAHAHEQVSSAGDQRWNELEAKVVMGVLRQIVESH
                     EFMDRLTQELEPGEPAVGIICMYSRQREVLDRMKAEASWLGDARKLVKIDTVDSYQGK
                     ENRVVILSTVRNNDRGKVGFLTSPNRINVALSRAMDRLFVVGSSAMWNRKNADLPLGR
                     VLSKVQQMSSEGKAITLSAKEFLQ"
     gene            26975..27217
                     /locus_tag="PL152_00870"
     CDS             26975..27217
                     /locus_tag="PL152_00870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47851.1"
                     /translation="MSGASYEFNRATFGLPVESYRVDSFITKGERLPVVTEFVLRLLR
                     ICGPITVPAFRDYFGFSEAESLSVVNRPGFRGGWLV"
BASE COUNT         4658 a         8813 c         9147 g         4614 t
ORIGIN      
        1 cttcaagctg atggccagca ccgccagctg cccgatcgcg gcgatggccg acgagacacg
       61 cggctactac gcggtgcagt tccaccccga agtcacgcac acgcggcagg ggcgcgcgat
      121 cctggagcgc ttcgtgctgg gcattgccgg tgccaggccc gactggatca tgaaggacca
      181 catcgccgag gcggtggccg cgatacgtgc gcaggtgggc ggcgagcagg tgatcctggg
      241 cctgtcgggc ggggtcgact ccagcgtggc ggcagcgctg atccaccgcg ccatcggcga
      301 ccagctgacc tgcgtgttcg tggaccacgg cctgctgcgc ctgaacgagg cgcagatggt
      361 gatggagatg ttcgcgggcc ggctgcatgc caaggtcgtg catgtcgacg ccagtgggca
      421 gttcctcggc cacctgaagg gcgtggccga ccccgagcag aagcgcaaga tcatcggccg
      481 cgagttcgtc gaggtgttcc aggccgaggc cgggaagatc gaaggcgcga agtggctcgc
      541 gcagggcacg atctatccgg acgtgatcga gtcgggcggc gccaagacca agaaggcgac
      601 cacgatcaag agccaccaca acgtcggcgg cctgcccgag accctgggcc tgaagctgct
      661 cgagcccttg cgcgaccttt tcaaggacga ggtgcgcgaa ctcggcgtcg cgctgggtct
      721 gccgcacgac atggtctacc gccacccttt cccggggccg gggctgggcg tgcgcatcct
      781 cggcgaggtg aagcgcgaat acgccgatct gctgcggcgt gccgacgcga tcttcatcga
      841 cgagctgcgc ggcaccgtcg accccgccac cggacgcagc tggtacgacc tgacgagcca
      901 ggccttcgcg gtgttcctgc cggtcaagag cgtcggtgtg atgggcgacg gccgcaccta
      961 cgaccacgtg gtggcgctgc gcgcggtgca caccagcgac ttcatgacgg ccgactgggc
     1021 cgagctgccc tacagcctgc tgaagaaggt ctccagccgc atcatcaacg aggtgcgcgg
     1081 catcaaccgc gtcacctacg acgtcagcag caagccgccc gccaccatcg agtgggaatg
     1141 attcggcgtc tttcagggtc tatcgaggcc tgtcggaaag ttcatctaag ttgttgtcct
     1201 acatgagaaa tgtctgtcga gatctatcgg catctatcgg tgggatgcct ttcgatctga
     1261 cggtaaatct gacggtagaa atcttcgacc ggatgtctcc aaagttttta ccgtcagctc
     1321 agagcgagca atgacggtaa aaaaagtggc atgaaattca tataaatcaa gcacttagtg
     1381 aagttgtagc ggccgctctg gcgtgacggt aaaattcctc cgagaggagg gactgccgat
     1441 gctgtccgat ggcacgctca ggggcatcaa gccccaggcc accacctaca agatcgccga
     1501 ccgggacggg atgtacgtga ccgtctcccc caggggcacg atcaccttcc gtctcgacta
     1561 ccggctcaac ggccggcggg agaccctcac gatcggccgc tacgggtcga aggacgggat
     1621 ctcgcttctg atggcgcgcg agcgctgcgt ggaagcgcgc aaggcgatcg ccgaaggccg
     1681 gtctccctcc caggagaagc agcgcgagaa gctcaggaca gccgaggcca agaccttcga
     1741 ggagttcacg aaacgctggc tggaagaagc caggatggcc gagagcacca agtcgatgcg
     1801 caagagcatc atcgaccggg acatcatgcc ggtgttccgc aaccggctga tggccgagat
     1861 cggcgcggag gacctgcgca acctgtgcgc caaggtcaag gcgcgcggcg cgccggccac
     1921 cgccgtgcac gtgcgcgaca tcgtcaagca ggtcttcggc ttcgcagccc tgcacggcga
     1981 gaaggcgccg aacccggcgg acgaagtcgg cccctcgtcg atcgccacct tcgtggccaa
     2041 ggaccgcgcg ctgtcgccgt ccgagatccg cgtcatgcac cgcgtgctgg acgccaccgc
     2101 gaccctgccg accatccgcc tggcgctgcg cctgatcctg ctgacgctcg tgcgcaagag
     2161 cgaactgatc gaggccacct gggacgaggt cgacttcgag aacgccgtct ggacgatccc
     2221 gaagagccgc atgaaggccg gcaagcccca caacgtctac ctgtcgcagc aggcgctcga
     2281 catcatggtg gcgctgcgca cctgtgccag cgggtccaag ttcctgctgc cctcgcgtta
     2341 cgacgccgat cgctgcatgt cgaaggccac cttgaaccgg gtcacccaga ccgtcgcgga
     2401 gcgagccaag gaagcgaagc tgccgctgga accgttcacg gtgcacgacc tgcgccgcac
     2461 cggctcgacc atcctgaacg agctcggctt caacagcgac tggatcgaga agtgcctggc
     2521 ccacgaagac gggcgctcat cgcgcggcgt ctacaacaag gcggagtacg cagagcagcg
     2581 acgtcacatg ctgcaggagt gggccgacat gatcgacgcc tgggtggcgg ggaagaccca
     2641 cacgccgacg ctgctgccgc cgacgatgaa ggtggcgacg acggcggcgc cgatttgagg
     2701 gctggatttg gacgcgatcc acaagcctga agcgccgctg tgcgtcggcg tggatggatg
     2761 ccgtgcggga tggatcgcag tcgccgacgt gggcggcggg ctgacatatc gattgcacgc
     2821 gagcttcgca gagctcctcg cttcgtggag tacagcccag aggatcctcg tcgacattcc
     2881 gatcggtcta ccttggcgag attgtccgtc gcgtccctgc gatcggcagg cgcgtgcatt
     2941 gcttgggccg cgagcctcga gcgtgttctc gccaccctcg cgtcgcgcat cgagggccgc
     3001 gaacgttgga caggctcgcg cgtggaacct cgatgaagtt ggccgaagcc tgtccgctca
     3061 ggcctgggga atctgcaaga agatcgtgga ggtcgatcgc gtgctgctcg aagacacgag
     3121 cgcgaaccgg aaggtctttg aggtccatcc ggaggtctgc ttctgggggc tgaacggcgg
     3181 caagccgatg cggcatcgga agagcaaccc ggagggcgtc agggagcgcc ttgccgcgct
     3241 ctcggttcgg ctgcccgcgg cggaccatct tctgcaaaga gttcttcgcg agacccgtcg
     3301 tcaagacgtc ctagctgatg acgtactcga tgcccttgtg gctcatgtca ctggacgcgc
     3361 ggtcgaagcg gagctccggc gcctcgttgg tgttccatcg gaagacgacg aaggcttgcc
     3421 aatgcagatg ctgttctgcg aatacgagtt gaccccgctc ccgccgggca gttgattggc
     3481 cgggggtgtt atccagcaga tgccactgtg gttctgaatc agcgaagcag gccgggtagg
     3541 ggtccgacgc cgttcaatcg acaccttcgc tgctgctgag cagtaaggtg ttcagcaggt
     3601 tcagctcgtt cgacgccatg aagtaccggt cgtcgaacac caggtccagc ttcagcggac
     3661 tgacctcgcc gactgttgcg gcccacggga acgactcgta cttcggcagc gggggcatca
     3721 cggccgagcc agcgttcagc gtcctcccga gtgcctcttg ccacagcggc tggtcgtcga
     3781 gccgaaaggg cttcccgtcc tcggcggtct cggagaacat gaactggtag tgcccggccg
     3841 gcaccgccac ccaggtgttc ggggatttca gctctttgag ggtgcggaac tcgttggcgg
     3901 cgtcgtgttg ctctcgctgg agcagcttgc gcaggttggc cgcggggatg cgcagcgccg
     3961 gcggaaactc ccttttctcg tccatggcct tgagttggcc catgagtgcg cggcaccacg
     4021 cgcgtcggca agacgcgcct ggcggagttg ctgatcgccc aggacatccg tcgcggcgag
     4081 gtggtgatcg tcttcgaccc gaagggcgac gtcgatctgc tgcgccgtgt cttcgcggaa
     4141 gccgagcggg cggggcgcgg cggggagttc ttcatgttcc acctcgggca tccggacatc
     4201 tcggcgcgct acaacccggt gggcagcttc tcgcgcatca ccgaggtcgc cacccgcatc
     4261 gcgggccagt tgccgtcgga aggccagtcc gccgcgttca aggagttcgt ctggcgcttc
     4321 gtgaacgtga tggcccgtgc gctcgtggcg ctcggtcgca agcccgacta ccaggagatc
     4381 aaccgctacg cgtccgacgt cgagccgctg ctgatcgact acttcgagta ctggctcgac
     4441 cgcgagccct ccgccacggg ctggcgcgag gagctgcgat cgctggccat cgacaagaag
     4501 aacctcgaca agggtctgca gtcgcgcggc gcgcgggcgg tgagcctggt ggagtacgcg
     4561 cggcgcaaga agctctacga cccgatcgcc cacgcgctcg cctcgacgct caactacgag
     4621 aagagccact tcgacaagct ggtggcgtcg ctgctgccgc tgatggagaa gctcacgacg
     4681 gggcgcacgg cctcgctgct ctcgcccgag ctcgacgatc cgaccgactg gcgcccggtg
     4741 ttcgactgga cctcggtgat ccacctgggc ggcatcgtct acgtcgggct cgatgcgctc
     4801 tccgactacg aggtggccgc agcggtgggg aactccatgt tcgccgacct caccagcgtg
     4861 gccggcagcc tctacaagtt cggcgccggt cgcggcctgc cgggcgaggt gacgccccgg
     4921 cggatcgcga tccacgccga cgagttcaac gagctgatcg gcgacgagtt cattccgctg
     4981 ctcaacaagg cgggtggggc gggctttcag gtgacggcct acacgcagac ctggtccgac
     5041 gtggaagcgc gtatcggctc gcccgccaag gccggacaga tcgccggcaa cttcaacacc
     5101 ctgatcatgc tgcgggtgaa ggaggtggcg accgccgagc tgttgacgag ccagctgccc
     5161 gaagtgcatg tggtctcgac cgtcgtgtcg tcgtcggttt ccgacaccaa cgaccctgtc
     5221 gatttcgcgg acttcgccag ccgcaacgaa gaccgcatcg ccccggaaac cgtccggatg
     5281 ctcgagccga ccgatctcgt gcaactgccc aagggcgagg ccttcgcgct cattcacggc
     5341 ggccagctgc acaagatccg catgccgctg ccttcggcgt cgagcgatcc gctcatgccg
     5401 acgagcctcg cggcgatcgg cgaaacactg cgtgcgcgcc tcgacgggcc ctggaatcag
     5461 ccggaggacc cctccgaacc ggagggtcga cgtgtcgtct aggaatgcgg ccttccacga
     5521 cggcgccttg gccggggtgc tgttgagccc gctgaagctg gccttctcgc tggccttggg
     5581 tctggtcgcg ctcctgctgt gcgcctggat cgtcgactgg gtgttcgtgt tcaaggtctg
     5641 gccgcagggg atcgagcgac tgcgtgaact gctgacgcac gatcttgcga gcggcatcgc
     5701 gctcgccgct cggcagggcg ctgatgccgg cgtcatcacc gcgccggcga atgccctcta
     5761 ctcgatcgtg ttcgaggcca ccggcatcca cgacatgggc acgcagttcg ccaacgggtc
     5821 agccctgtcg atcccggaca cgatcatgcg gcgcagctat gtctcgcacc gggagggcat
     5881 cgaggcggcg atggtcggca cgcagttgct cggcgtgcgc gcggcgatcc tggcgcggtt
     5941 cgcgccgctg ctcctgcttc tgtatgcggt cggcgccgcc gatgggttca cccagcgtgc
     6001 gatccgccgg gcttgcggcg gacgcgagtc ggccagcctg taccaccggg cgaagtacct
     6061 gcagttggcg gtgctggggc tgggcggggt ttccctgttg ctctggccgg ggccggtgca
     6121 gtgggagcgc tgcgtggcgc tgggcgcgct gctggcaggc ggcttggcga gtgtgcagtg
     6181 ggcgtactac aagaagcaca tgtaggccgg gcaccgtggc gtgcgcgagg ggcccggccg
     6241 gtttcagaag tgccagaagc tcggcgcctc cggctccggc gcatcgagga tcgggtcgct
     6301 tcgtcgcacg agcggatcga gccagtccgc cttggccttg gcccactcga tccactgctg
     6361 cttcgcgggc gtgagctcat cgtcgtggcg tgctcgatcc tcgacggcgg cgatgaactc
     6421 gcgcaaccgg ttcgcgcgct gcagccggct cgcgtcgcgg aacagggcgc gcagctggcg
     6481 acgttcctcg ttgcgcgcgc gtacctgcgc ttcgtatcgg gcgcgcgctt cctcgtaggt
     6541 ccgccggcgg cgctcctctt cctcctcctt cgcgcgtttc gcttcggcca ggccgacgat
     6601 ggcagcgacg atgccgctga gccgcgagtc gatgaggctg cgttccgtgt cgttccactt
     6661 ccgtgacggc cacgatccca ccgtgagcgt cagccgtccg gtggggtgcc aatcgaactg
     6721 cgggatgttc ggatactctc ctcgggcgcc cacgcgaaag gagtcgtagt accggtcgcg
     6781 cgcccgcacc tccgcgcgtg tcggcgtgtg gctggttcgc gtgacctgct cgacgatcga
     6841 gatcgtcagc gtcgtgccgc cgccgaccag cagggtctcc cccttctcct cgtccacccg
     6901 cgccgtgaag ccgctgggct cgagcgactt gagcagcgtg tccatcagca gaagggcgcg
     6961 gtcgagggtg ttcctcgtga cctgcaggtc gagcacttcc ttcggcgcgc ttcgcacgcc
     7021 cgccgggtga tcccagccgt cgcgccgctt caagcgtgcc gctgtggccc gtacgagcgg
     7081 gtgcgggtcg acgagctcgg cggggacgct gacgggcgac tggctctcgc gggtctgttg
     7141 gagggcgtca cggacacgcg cgtgcatggc cgcctcctgt tcggacagcg gtatcggtcc
     7201 ggcgagatcg cgcgcgccgg cgggtagggc gggcagcggc accttgcggg tggggcgacc
     7261 ggccttgacc ttcgcccagt agccccgcgg cggcaccgga atgccgagct tcttgcagat
     7321 cttgaccagg cccacgtcgg acaggccgta gcggggcgcg acgaccgtga ccgcgtcggt
     7381 ccagacctct tcgtacagcg cctcccggct gacgggggtg ccttggcgtc tcatcgcatg
     7441 tctcccggtt cgggccggag ccgcttgggg acggcggctc ctgttggttc gaagatttgc
     7501 tgttcagtaa atattgccac aatgccgaat cttcggtatg gtgcgcatat ttactgcaca
     7561 gcaaacctag gcggtgacca tgcttcatgc gcaggcgagc gaacgggagc tgatcgatca
     7621 gttcctcgct gcacttcggt cgctgcctga agtgcaggcc gaactcgaac gcgtcggctc
     7681 gccggagctc gacgcccagc ttgcgctcga cgtcgcgggt aagcctatcc acgcgctcgt
     7741 cgagctgagg aaggccgtct atccgcgcga cgtccgtcag ctcgtctggc ggatcaggga
     7801 ccttgcccgc cagcaacccg ccggcgagag cggcagcgag ccgcttgcga tcctggtcgc
     7861 cgaatccatc tcgccgggcg cgaaggagct gcttcgagcc gagcgggtcg ggtactacga
     7921 cagtgggggc agcctctacc tgccggcacg gggagcctac ctctacgtcg atcgaccgcc
     7981 gcccaagtcg ttgtcccgct cgatgcgctc gctgttcacg gggcggcgcg cacaggtcct
     8041 gcacggcctg ttggttcgcc accaggactg gttcggcgtg aaggacctgg ccgagcagac
     8101 gctggtctcg tccgccacgg cgtcccaggt gctcacggag atggagcgct tcgactggct
     8161 ggagtcgcgc gggcagggcc ccagcaagca gaggcacctt cgggaacccg cggcgctgct
     8221 ggatgcatgg gcgaagcagc tgagctcgat ccggcccccg gcgatgcgac gctacttcgt
     8281 gcccgcgatg aagcccgacg gcctgatgga gcgcctcggt caggtttgcg aggcgcatag
     8341 cgtcgactac gccgtcagct tcgaggccgc cgcgcagcgc tacgcgccgt tcctgtcggc
     8401 ggtatcgcag gtccgatgcc gactgctggc ggggtcgggg ggcgatgccg cgatcgcgga
     8461 cctgggcgca cgcgtcgtca gcgagggcgc gaatctcgcc gtcatcgaag cgaagtcgga
     8521 cggtgacctg ctctttcgcg agcgggtcgg cggggtctgg ctggccagtc cggtccaggt
     8581 gtacctcgac ctcctccgcg gcgagggtcg agccaaggag atggccgaac acctgcgtcg
     8641 agaaaggatc ggcttctgat ggccaagccc gcaaccttcg acgggtacag cgaccagtac
     8701 acgctcgact gcgagcgggt gctggtgacg ttgctgcggg gactgggacc gtggaaggac
     8761 tcggtctatc tggtcggcgg cttgacgccg cggtatctcg tcccggctcg cccgccggtc
     8821 gtgcccgcgc acgccggcac gctggacgtc gacatcgtca tcgacctgca gatcctggcc
     8881 gacaccgagg cgtaccacac gctcgaagag aacctgaagc gcatggggtt cgagagggcc
     8941 gagaacgagc aaggggccaa gctctcctgg cgttggcaga cccggaccga gcacggcgca
     9001 ctgatggtgc tggaactgct ggccgacgcg ccgcagatcg ccggaggccg ggtgcagccg
     9061 ctgccgacgg aaggcacgat ctcggcgctc aacattccgc actcgtcgat cgtcttcgac
     9121 ctgtaccagg tcgccgagat ccgcgccgag ctgctcggcg aaaacgggat ggcgaccgag
     9181 cgcgtcaagc acgccgacat cgtcagcttc acgtgcctga aggcattcgc gttcgaccag
     9241 cgcttcgaac gcaaggacgc ccacgacttg gtctactgca tcgagcatgc cccggagggg
     9301 ctggacgccg tcgtcggggc cttcacccgg gcgcttgcgg gcaagcacgc ggccgtggtc
     9361 cgagaggcgc tggacatcct tcgacgacgc ttcgccgacg aggaagcgac cgagggctat
     9421 cgcaaggacg ggccggtcgc cgtggccaag ttcgagctgg gggagggtga cgaggcggat
     9481 cagcgcgagg cgagggtgtt gcggcagcgc caggccagcg acgtgatcga tcgactcctg
     9541 gcaggcatcg gctagcggct gaggcgacga gatgctcatc acctttggat tacgactgga
     9601 cggagaacgt ggctggcgcc cagcgaatca gctggggagg ccggtgttcg gcccgctggg
     9661 gctgctgaat ctgctcgaga cccgtctcgg cttgctgcgt tccgactgcg cgcacgcgca
     9721 gcgcgtcacg cagtaccgcg aatgcctcaa acaattcgac acgccagatc gcttctatca
     9781 cgcctcgttc ggcatcgatc cgatcggtac ttccgcgtcg ctgctgtcgt ggcgcgatac
     9841 ctggcatctc cacggctgga acggccagtc gccgccggcg gccggtgtcc ggatgaccga
     9901 catggccgcc gtcgagtccg ccgcgcgcga acttttgttc ccgtcgatcg gcgagcgact
     9961 ggcccgagta gccgagttcc tcgctcgaca gcaatcaagt atcgacggca tcgagctcgt
    10021 cgacccgctg gatgcctttc ccaagcgttg gcgggaggtg ctcgcaaagc tgccggtacg
    10081 gtcgatcagg gcgtacggtc cgtcggcaga cccgatcacg gtgctgggca ggctccagat
    10141 cgcgctcaag gctgcgcaca aaggcgagaa gcctgaaggg aagatcgcct ggttggacga
    10201 cggcagtctt cgcgtcgtgc gtgcggagac gggcctcgtc gccgcgcgct gcgtcgccga
    10261 cgatctcgca gcgccgcagg agggaatcgc gatcatcgcc gagcagagcc gttcgctgct
    10321 cgacgccacg ctcgatggct ccgatgtggc ccggcagggc ttcctggacg cgagtcccct
    10381 cgcacctgcg cttcaagtcc tgcctctcgc gctcgccacg gtctgggagc cgctcgacgt
    10441 ctacgcgctg ctccagttcc tctcgcaccc gatcgggccc gtacccggtt atgcccgccg
    10501 acggctggcc gaggtggtgg ctcagttccc cggcgtcggc ggcccccgat ggcgggaggc
    10561 ggttgaggag atcgtgcggc gccacccgga gcgtgccgac gatctccgtc gggcgctggc
    10621 gttctgggtc gagcacaagc gcttcgaccc caagcagggt gcgccgacct ctgcgctgct
    10681 tgaaagaacc cgggcaattc gcgactactt cggcgcgcgg ctcgcggatc aggacgccaa
    10741 tcgcagcgca gctgctgcaa cgggtctcgc acaggccgcc gcggtgaccg cggccctcga
    10801 ggcgctcgca gcgcagggcg aggcctcggt cgccccggtg gagctcgagg cgcttgtggc
    10861 gcagtgcacg gcgcgaggtg cgcccaactt cgctatgcac gcgcaggtcg gctgcgcacc
    10921 ggcagctacg gatccggggg cgctgctgga gcccttcgac cgggtgatct ggtggcagat
    10981 gggtgcgccg tcgttgccgg gtcactaccc ctggagtgcg agcgagatgg cgtcgctgtc
    11041 ccgtgcgggc gtggaactcc cgccgctcga agagctactg acgcggcgcg gcgaggagtg
    11101 gctcaggccc gtcttgcacg cacggcggca actcgtgctc gtacttcctc cgtacgggga
    11161 ggagatgcac ccgctgtggc aggagatcca gtggttcgtc gatggcgtac ggcccgagcc
    11221 tttggagggt ctcctgaccg gccgcgccgg caacgaactg ccaacgatcg cgcacgcggc
    11281 gctgccggcc cgcaggcgtt ggtgggactt ggcggcgcaa gcgacgattc cgccgcgcac
    11341 ccgcgagtcc tattccagcc tcagcgcgtt tctgaatgcg ccccatcagt gggttctgaa
    11401 gtacgcggct cggctcaatc cgtccaacct cctcgcggtc gccgatgcga acttgctcta
    11461 cggcaacctc gcccatcggt tgatcgatcg gttcttccgg acgagcggtt cgctcgcgct
    11521 tcgcggcgaa gcattgaggg cctggttctc gcgcgagttc gagacggtcg tggccgaaga
    11581 gggcgcggtc ctcctcatgc cagggcgacg cggagactac gagcggcttc gctcctccct
    11641 ggagcgcgcc atcgcggagt tccagcggca gttcgcggcg gcgggcgtcg atgctgtcga
    11701 gcccgagcgc gcgctcgccg ggagcttcac cggcggcgag ctcgagggcg tcgccgatct
    11761 cgtcgtgcgc aaccggtccg gccagcgcgc gatcgtcgac atgaagtggt ccggcggcaa
    11821 ctaccaccag gaacggctcg cgaagaatcg ccatctgcag ctcgcggtgt atgcggagct
    11881 gcttcggcag gagacggggg cgtggccgca ggtgtcctac ttcatcctcg aagcagcgcg
    11941 cctcatcgcg ccggacacgg ggttctttcc ggaggcgcgg gcggttcagc aaggcggccc
    12001 aggatccacg gcggtgctgt gggatcgatt cgtcgcagcc tggaactggc gccgctccca
    12061 gatcgatgcg ggcttgatcg aagttgcggc ccggggcatc gaacctaccg ccgaatcggt
    12121 ggggccggcc gaggcgctcg aaccggaaga gctgcgggag gactacgacg actaccggtg
    12181 gctgacgggc tgggagggct gagcgatggc cggcaacctc aggttcgtca gcgcgggggc
    12241 gggaagcggc aagacctacc gcctcacgca gatcctgcac gagaagctca gctcgggaca
    12301 ggccaggccc ggcggggtga tcgcgacgac cttcacgcgc aaggccgcga ccgagcttcg
    12361 cgagcgggtc agaacggcgc tgatcgagaa gggcgagttc gcactggcca attcgatcgg
    12421 acaggcgcgc atcggcacgg tgaacagcgt ctgcggcggg tatctcgagc gctttgcgtt
    12481 cgaggcgggc ctcgccacgg agcagcgcgt gctggaggag gcgcagtcgg gcgcactcgt
    12541 gagggaggcc atcgacagag tctccgacag cgcctccgtg caggaactgg tcgagctcgc
    12601 gcggcgcctc ggcatcgaga gctgggaaga cgcgctcaag tcgatcatcg accaggctcg
    12661 cgcgaacgac atcgcgcaca cgcgtctcgc cgcgttcggg gaacgcaacg cgagggaact
    12721 tctcgcccac tttcccgcgc cggcgacgga cgacctcgac gcactgctcg tgagtgcgat
    12781 cgaggccgcc gtacccgagc tcgagaagcg cgccaccggc aagaagaaca cggccgactt
    12841 catcgcgctt gcgcgcgaaa cgctgcgttt ggtgcgcggt ggaaccgcca cttggtccga
    12901 ctgggtcaag ctctccaaga gcgcgccgga ggcgggcctg aggacggtcg cgcagccagc
    12961 gacggacatc gccggtcgct tcccggcgca cccacgcttg cgccaagaca tcgagcgcta
    13021 ccttgcggcg atcttcgcgc tgggcgcgag ggcgctggat gcctacgccc agagaaagcg
    13081 cgagctgggt gtcatcgact tcgtggacca ggaacacctc tttctccgtc tgttggagaa
    13141 acccgcagtc gtcgaggctc tctcggagga actcgatctc cttctcgtgg acgagttcca
    13201 ggacacgagc cccatccagc tcgaactctt cgtgcgcctg tcgcgcattg cccgcgagag
    13261 cgtgtgggtc ggcgacgtga aacaggccat ctatggtttc aggggcagcg acgccgagct
    13321 catgaaggcg gtgctcgccg agcttccggc gatgggcggt acgaaggagg tgctcggcca
    13381 gtcgcggcgt tctcggcctg gcctggttcg actggtcaac ggcgccttcg gcgacgcgtt
    13441 tgcgcccggg ctctcgcgcc aggaggtgga actggcgccc gtgcgcaaag agctggtccc
    13501 cgacgcggca ctcgcgaact gggtgctcaa cggtaggaac gtgacggagc gggctgccgc
    13561 actcgccgaa ggcgtcaaag ctctcgtcgc ttcaggctat cgcgtcgtcg acccgtcgag
    13621 cggcaacccc cgacccgctc acttcgggga catcgcggtg ttgtgcaaga ccaacgtggg
    13681 cgtccagacg gtggcggagg ccctgcgcat cgcgtcggtg ccctgggcca cgcagcagtc
    13741 cgggctcctc tccacgcccg aggccgtcct tgcggtcgcg tgcctgcgtc gattcaacga
    13801 cccgcgcgac accctggcga gcgcggagat cgtttcgctg gccgactgcg aggagcccga
    13861 gtcctggctc gcggaccgcc tgcgctacct cgaagccggg ggcgacagcg cgcagtggcg
    13921 ggacggcacc gagggcggtc acccgctgct cgcgcgcatt gcgaagctgc gagagcaggc
    13981 gccgctgctc tcgccctacg ccgcgatgga gctcgtcgtc gtccagtgtg atctcgccgg
    14041 acgcgtgctg cgctggcggc gcaacgaact cgtcgcccag gtgcgcttgg cgaacctcca
    14101 ggctctcctc ggcctcgcta ggacctacga agacgcgtgc cgtgcgcggc gcgagccggg
    14161 tacgttttcc ggcctgattc tctggctcgg cgagcaggcc ggtgcgcaac tggacatgct
    14221 cgcgcaaccg cccgtcgatg cggtcaagat catgacgcac cacgcggcca agggactgga
    14281 gtggccgatt gtcgtcctgc tggacctgga gaaggacatc caggaccgca tctggtcggt
    14341 cggcgcgcgg tccgaggggc gcctcgacgt cgccgccccg ctcaaggacc ggtggatccg
    14401 atactggccg tggccgttca gcgcacagaa gaaggtcgac atcgccgaca ccattgctca
    14461 gtcggcagag ggaactcgcc tgcgtgcgga ggcgatggaa gaggccaagc gcctcctgta
    14521 cgtgagcgcg acacgggcac gcgactgcct cgtcttggcg ttccccgcca agcgaggcgc
    14581 gtgcgagtgg ctcggttcgc tgaatgcgcc gtggctggcg ccggacgtcg cggacgatgc
    14641 ggtgtctctc cccgatggtt cgcgcgtctc gtgcgacttc cgcagcttcg acgcgccggc
    14701 cgcgccctct gtcgcggtcg aacccgatgc gccgctccat tggttccgtg gcaccgaatc
    14761 gcgcaccgag cggctgccag cgaccttggt tgcctcggcc acccaggccc gggcttgcag
    14821 gatcacggat tccgcatcga tcggcgatcg catcccgctc tcggcgggaa tcgacatgac
    14881 cgctctcggc gacgcgatcc acgcctgcat cgcgacggcg ttcaccgatc cgagcgtgcc
    14941 cttcgacgag ccccgggcag cgcgcatcct cggcggtttc ggactcgcgg gcgcactcga
    15001 accgtccgca ctggttcgac agatcgcggc attggatgcg tggatctccg cgcgctggcc
    15061 ggactgcagg cgccacgccg agatacccat cgagtcgatt cttcccagcg ggcaggtcat
    15121 gcacggccgc atcgatctcc tcctggagac gaagactggc tgggtgcttc tcgatcacaa
    15181 ggcaaatccg gcgccgcggg atcgatggga tcaggttgcc caggagcacg gcgggcaact
    15241 gtccgtctac gcggatgccc tcgtgcgtgc cacggggcgt tccgtcgcgg aggcgtggat
    15301 cgtcctgccc gtcggagccg gcgcgatcca ggtgacggcg agcgagctat gagcggcaga
    15361 ccatctcatc ggcgagagtc cagggcgacg ccaagcacgg agcggcgccc agcggtgggg
    15421 aggcggccgt gaggcacgac gcggtgacgt ggctggactt ccgcgcggac gatctcgcgc
    15481 gagcgcgcga cttcatccga tcccttcagg aagaaggcgt tatcgacgag ctcgggttcc
    15541 tcgctcttct gggacggttc tccgacctgc tgcatccggc gacgaccacc ctgatgcggt
    15601 ctgcccgata tttctacttc atcgccggca tctatcggtc gctcgagcgc gaaggggctc
    15661 gtgcgagcca gattcctcat ctgtctcggc aacggcagga cgagctgagg gacgtcctct
    15721 cggatacgga gacgataggc gtcatcggtc gggacgcagg catcaacctc aggcagttcc
    15781 catcgcagat ctactggagc gggctgaaga aactcgggat gttcacgtcg aatctctccg
    15841 aaagcgccta tcaggagcag ttcgacgaca tcaggcgcag ccgccgcggg tatcaggacg
    15901 acgacaagac gccgcaggga ccggagggcg tgcggttctg gtccgacgat ctgccgccgg
    15961 caagttttct ggattcggat ggccggatac ggccaggcgc gaccttcaag ctcaccaggg
    16021 cggaagcctc ggacctgcac aagaggttta cgtcgcgttt ctccgagtcg ctcctgtcgc
    16081 acatgctcaa acatcgactc atcggcatcg actggccttg ggagtgcccg aagctccccg
    16141 agagtctcgc cgtctgggtc tggcacgcca ggaacatgag cctcctcgtc cgaggagcca
    16201 cactgcagta ctactccctg ctgatggagg agcgcgacaa gctgaggatc gaggaggaca
    16261 cggacctcgt tgctcccatc ttcaacgagt ggtggatcgc ggccaaagag cctctgaaga
    16321 agtggcgcac gagcgagttc gtgacggtcc cgaccgtcgc ctcggcattg cgtctcggca
    16381 accgaggcga tcgctggttc atcgacggct ggctcgagtg ctgtgtcgcg gcgtcgactg
    16441 ccgaagcact tctgcgcgac gagaaggcca ggcatctgat ccgagagcga gagcgacgcg
    16501 tcaagccggc gaaagcgcgg ctcaagtacg agaagcacct gaggcagtgg aagcccgatc
    16561 gggtcggcga agggatctac cagttcgact accgacacag catcgggtcc cgcttcgttt
    16621 ccgagatcct cgacggcatg gagcgcggcc agtgagcgtt ctcgacgtta ctcgccaacg
    16681 cagtgcgttc accgacgcac tgcgtccccc gcctggattc gtgctcggcg cgtgcatcgg
    16741 cacgaccttc agcctggact tcgagctctt caccgccgtg ctcctggcat tcgtcggggc
    16801 cgacgtggag gaacccctga acaacgcgcc tgcagtgctg acgtcggtgg cgaggctgag
    16861 gtctcgcctg cgagtcttcg tgaacggggg aagccttcac ccgccggtga cgacgaaccg
    16921 gctctacgcg ctctacgacc gaatactccg gccgaaggca gtggacgggg gcgcattcca
    16981 ccccaaggtc tgggtcctgc gtttcgatcc ggtggcccgc cccgagcggc acagcgtcga
    17041 gcccgtttat cggttgctga cggcgagcag gaacgtgacg gattccggtt gctgggagct
    17101 cggcgcactg ttcgaagggt cacggcgaac cggcacacag aagttcggat cggacgtcgc
    17161 cgccttctgc cggaaagtgg cggcttcccg cgatctcccc aaggctttgt ggaagctgat
    17221 cgaggagctg aagtacgtcg agttcgtggc acctcgggag gcggcgcagg gcctgagatt
    17281 cgattggcag tcgcctggcg atcgcgtgct catcaaccgg ttgccgcggg ccatgtcgcg
    17341 cgtccttgtg atatcgccgt tcctgcgcgc ggacttcctc aagcgtctgt gcgatcggac
    17401 cgacgacctg acgctcgtca gcacccagga cgaactcgat cagctctccg acgagactca
    17461 cgagcgtctg gctaacgccc gggtctttgt ggtctcagcc gaagccgacg aagaggcgcc
    17521 gtcgctggac cttcacgcca agctgctggc ctgggagtcc gatggcgtca gcgaaaccct
    17581 gatcgggtcg gcgaacgcaa cgggtccagg ctgggggtgc ggtccgaacg tcaactgcga
    17641 ggcgatggtg tcgctcaacc ccggccttgg catcgatgcg gttgtgaaag ccttcgtcgc
    17701 gcctgccaag gaccagctgc aaccgtggat cgacaggtac gtccgccaga ccgcggtcgc
    17761 cgaccccgag cgggaggcgg ccaagcgcct ggagcacttc aaacgtctcc tgcgcgcgga
    17821 ccagattcgc ggcgagtacg acccggtgaa gaagaccctt cggctgctcg cgccgtcctc
    17881 ggccggcgag cagacgtggc cggcggacat gcgcgcggag atcgtgccac tcctccagcg
    17941 agatcagtcg gcacgcgcgg actaccggct cgtttacggt ccgggggcgg ccttccaagg
    18001 gatcgagctc gaggacgtcg cggctttcgc ctacctcgtg gtctggagcg agcaccaccg
    18061 ggacgtcgaa ctcgcggttg tcgtgcagtt cgagctgaac ctcggagcac tggaggcgga
    18121 tgaccgggac aaggccgtga acgcccgcct tcttgaggga gtcgacgcgc gcagcctgct
    18181 gttagacgtg ctggcgggct tgcccggcgg gacatcgcgg cccgcaggcg ggggacagtc
    18241 cggggtctgg caggcgggcg atccgctgct gaggcgagcg acgatcgaaa gaatcctcga
    18301 ggcgtgcact gcggacccga gccgcatcca cgaggtcgag gccgtcttgg cggcgtgcgg
    18361 cgacgcatcc aacatgagca cgttccgcga gttctgggcg gtgttccgcg aatcgctcga
    18421 agaggaaggt gccggtgtct agtctcgagc tcgccgagtt tcagcgcgcc gcggtcgaga
    18481 cgatctgcga gcgtttgacg aacgcaggcg gatcgcgtcg atttcttctc gccgacgaag
    18541 tcgggctggg caagaccgtc gtcgcgcgcg gcgtgatcga cgaactcttg cggcgccggc
    18601 ggggccgaga gctggtggtg gtgtacttgt gttcgaacac cgagatcgcc gaccagaacc
    18661 gaacgaagct tgcgccgaac gctccgccgt cgctgcgaag gatcacccag ctcgcgtgga
    18721 gcaccaaagc cggcgacaac cttcagctct attcattcac tcccggcacg tcgctgtccg
    18781 agggaaccgg attggcctgg gagcggcagc tgctgctgtt ccttgttcac cgcgtcctcc
    18841 ggcaggacat ccggaagggt aagtggcggg agtatttccg atgcggcgcc ggagaagagc
    18901 gctggaaggc ggccacgaag ttcgccgtcc tgcggctgga gttcaatcgc aaactccgcc
    18961 ccgggctgca gcagaggatc gccgaggagt ggagaaagcc gaccgaggtc gacggtacga
    19021 cgctcgtgct gtccgaagtc ctgcgcggcg aggtcgacca gttcaagccg accgatgccg
    19081 gaagccggag gcgccggaac ctgatcgtcg ggatgcttcg cagcgccgcg cagcgcgttc
    19141 tcctcgacga tctacagcct cacctcgtca ttctcgatga ggtccagcgg ttccgggaag
    19201 tgattgagga ggcgaccagc cgcaggtcca tcgcggcgcg tctcttcgag aggcgtccgg
    19261 gcgtcctcat tctgtcggcg acgccctacc gcatgcttgc cctggaccat gaagggcagg
    19321 ggcactacga cgagttcctg gacacggtgc gtttcctgtt tgcagaccaa ggcaagcagg
    19381 aggtcgctcg cctgcggagc gatctgaagg cgtttcgcga acgcctcgaa ctcggggcct
    19441 tccttcgagg gcacgatcac gagctgctgg cgcttcgagc gcgcatcgag cagcgcctcc
    19501 gcaaggtgat ctgccggacg gagcgcaacg cgtacataca cgacccgacg aagggcgtac
    19561 aggaagtgcg gccgacgggg aacgtgttcg ccgtgcctca gaaggaagag gtcgtcgact
    19621 acatccgact gcgaaggttc ctcctggaca aggtcgacac ctcccagcac atcacggagt
    19681 actggaagtc gtgtcccgcg cctttcacgt tcatggacgc gcagtacgcg ccgatggcgg
    19741 cggcgaagcg aaagaaggcc gtagttccgg cgggcgtcat cgagcaagcg tcgaaactgg
    19801 gcgcgttgcc gcgtcggaac ttgcggttcc gagagctctt ccaggcgatg ttcggcggtc
    19861 ccgacgagcg ctggcggttc ttgtggacgc ggccgaccta cacctactac cgcgacgaat
    19921 tcttccggga ggccgatccg aagaagatgc tcgtcttctc cggctggcgc ttcgtgccga
    19981 aagccatcgc tctgctgaca agccacgagg ccgaacaacg aatcgctccg cgcggtcgcc
    20041 tgtgggaggg cgacgaccgg ccgccactgc gcttcaccga gaagggctct ttccacatct
    20101 tcgatgtctg tttgccatcg ccggcgctcg ctcggctcgt cgaaccctcg gctctggcga
    20161 gcgacgccct cacggccaag gaactgctca ggcgcacgcg gaaggctctg aagagggcgc
    20221 tggaagaggc tggcgtcgag gttgcagcca cgagccgttc gccgatatgg caagccgtcg
    20281 cgcgcctgga gcgacactcg gattcgtcaa tccgagatgc tctggaaggg agcgcggtct
    20341 acaacgggga cgacatcacc gagcgcttcg ccgagcacgt cgataccttc gtcgactgga
    20401 tggaggagga gggctccttg cgcatctccg aggagcggct cacgcatctt gcgcgcatcg
    20461 ctgccttttc accggccatc tctctcctgc gcgccttctg gacgacttat cccgaaagcc
    20521 gcggcgaggt gcacgaaagg cttgtcgatc tctgctttgg agagctccgg agctatttca
    20581 accggcgcac cgttcgagcg atcgtcgagc gctccgtgcc ggcagggcgc ggctatgcgc
    20641 gtgccgcgat cgagtactgc gagcgcgcgc acttccaggc gattgccgac gagtacttgt
    20701 acctcggcaa gaacgttctg cagcgcaacg gcccggccga aatggcggaa cacttggctc
    20761 gcgtgctggg cgtaggcacg gggagcccca acatcaatct gacgaccgat acggggcgca
    20821 tacgtgccga acaactggtc cgccggtcgc acttcgcgct ggcgttcggc gaggacgtca
    20881 aggtcgacca aggagtcccc ttggagcaaa cggggcctag ccggaagacg gcagtgcgcg
    20941 aggccttcaa ctcgccgttc tggccgtttg tcctgtcgac cacgtcggtc gggcaagagg
    21001 ggctggactt ccacctcttc tgccgcgaca tcgtccattg gaacctgccg tcgaatcccg
    21061 tcgacctcga gcagagggag ggccgtatca accgacgcga cggattggcg attcgtcggg
    21121 cgatcgcaag agaatggccg ctagagcgcc tcgcccaagc gggcccggga agcagcccga
    21181 acttctggga gcgagtgttc tcggtggtcg gggaggcgcc cgatattcag cgctacaagc
    21241 acgggctcta cccccactgg gtcttcgagc cgagaggcgg ccaggtcgag aggatccgcc
    21301 ggcacctctt cttctacacc gacagtcagg acgcgagccg ttacgaagag ctcaaggaac
    21361 gacttgctct gtaccggttg gtgttcggcc aaccgcgtca gcaagatctg ctcgagcgga
    21421 ttcagcgcac cgttcgcgac gaagccggcc gggaggtgag ccacaccgca ctgacccgct
    21481 acatggtcaa cctctcgccc ttaggggaag agcatgcgca ggttagggcg gcgcttgaag
    21541 cccaagagat cgcggcatcc cctgacaagc tgcagcagct cttgcgagat gtcgaacaga
    21601 tcgaggccga gcgtgcagcg gaactgcttc cggcgagagg cgatctggac cggttcaaag
    21661 cgatggttcg gcggcacatc gagtcaccct acgaggacca cggcaagctc gtcacggcga
    21721 ttcgcgttct gacctacctt cgcgatccgt acgacgccat catggatcag tacgtcgatc
    21781 tcggattgca ggacgacgtc gcgctgatcc gttctgcctc acggtcgctc gcctcctgtg
    21841 gaacggtcgg ggcgaagggg tgaggagatt ggctacggtg agtgaatcct atcgatgcca
    21901 ggcgggaggc gacttcgtgc gcccccctcc accattgaca tctttgaaga ttgaaagcta
    21961 ttgcggagcc tcgaagaggc gcctcccact gacggcacgc ggaacctttc gagcgagccg
    22021 tcgcactgcc aacactgcgc gactgtcatc gccaagtcga taagtagcta aagatggaaa
    22081 tacgcttcct cagaaagtct ggcgtccccg cggcggagat cagcgcgcac cagcggattc
    22141 agaaggtctt cgacgcttcg cccttcacca agtcttggag gggctacgcc tcgtttcagc
    22201 tcgcgcgtgg cgggcgaggg tccgggggcg atgacttcga cctcgttctg gtcacccaca
    22261 cgtgcatcgt cgtcatcgaa ctgaagaact ggcacgggaa tacgctcaag tccgaaggcg
    22321 gcaggtggta cgtcgacggc gaagaccgag gagcgtcgcc cgtcgatctg gtcagcgaga
    22381 aggcgaaaaa gctcgccagc cggatgaagc agaagctcgg cgtcgagaag acgccgttcg
    22441 tgaattcgtt cgtcgtcatc caggacggca ttgaaaacct ggaactgcgt gacgaggagg
    22501 aatcgcgttc agttctcttc ctgaccgatg acctgctcga gtggacaaaa cgcgacgcgt
    22561 acgaggccgt cttgccgtgg cggccaagat tcaacgttct ggcgaacttg ccgctgtacg
    22621 accagttctt cggtgggacc gacttcaaac cgaaggacta cttggttcaa gggtaccgtc
    22681 ccggagacaa cccgatttgg gaacacccac ggcgcctcta ctcagagttc cgtgcgcggg
    22741 cgaaggacaa ccccgaccag ttcgccctct tgcgacagtg ggacttcagc gctatggggc
    22801 ctgccctcat cggagagggc gacagggcgt tcatcggttt gcgcgagcag cgcatctatg
    22861 agtacgtcgc ggagcgaaat gaggagcttt ctctgggtct gctcaggccc ataggccgcc
    22921 acggcgacaa ggacgtaaac ctcgacttcg ccgaactcta tgccctccca tcgaagctgg
    22981 tccgccttaa cgagttcagc aacagcgttc tgcccagact gacggcggac gagcgcgttc
    23041 ttctggtcaa ggcgctcttg aaccggttcg ccgaacttca cgagctgagc gttgcgcacc
    23101 gcgacgtggc ggagcactgt ctgtggctgg agcggccagc caaggtcgtc atgaccggct
    23161 ttcccgccgc ccactatccg tccatgcaga cggtcggcac cttccgtgaa cagctgaagg
    23221 ttgagcgcgc caacctcccg gaagacaccg gacttgcaaa ggagggcacc ccgtacacgc
    23281 gagacgtcta cctactcggg gtgcaagcac acctgctgtt gttcggcgag cgtccgccaa
    23341 agttgcggga cgtctacgag tggacgccgc gagccgataa ggacttcggt ggggcgttcg
    23401 acgctgtatt ggccaaggcg ctcgaccgcg agccatccaa gaggtacagc aacgcacgag
    23461 agatgctcga ggccgtcaac gccgcgacct catcccccgc tcaacaagcc atcgatctga
    23521 gccgattcga agcgtatcgc gcagcgagca agcagcgcga ctatgaagag gcagaggtgt
    23581 tgaccgatga ggatgatctt ctcttctatc gatccaaagg agccgagggc gacctggcgg
    23641 tgaaggtctg gattggcgtc gagcccgacg ctcgaaagcc cgaactgtcg ctacgcctgc
    23701 tcgccttcct cgagcgagca cgagtgctga agggctgtgt ccttcccggg ctgccacgca
    23761 ttatcgattt cggcctcagc cgccggagcc ttctgctcgt ctacgagtgg gtcgacgggc
    23821 ttgcgctgaa ggactggctg gcaaaggacc cgccgctcga gcagcggctc gcaatcgcat
    23881 cgatgctcgt cgacacgctc gcgcgtatcc acgccctcga actggctcac ggcgacgtcc
    23941 accccggaaa cgtcatcgtt gctccgactg gtcaggcctt cttcatcgac gctttcgact
    24001 tccgtcgcgg aacagaggat gcctacacaa cggcctactt gccgcccacg tacaagtcgc
    24061 tgacgccgat ggaacgggac cgctatggag tggcagcggt catcgctgag gttctgggag
    24121 gcaccaagga aacacccgaa ggtcccttcc ccgtacctgc cgtctacgag cagctgcgca
    24181 agcttctcac tgatctgcag gcctctgcgc ttgagccatt gcagaacgcg ctggcaaagg
    24241 cggcgcaggc gggcgagaag gcgacacccg cgttcacggc aacggtgcag tacctatccc
    24301 gcaagggcgt ccctgccggc gccatgcacc cggataacgg catcttccat gtgtcggtgg
    24361 agcgctcgaa gaagagggcg ggcgatgccc ctctctggtt cgttcgcatc acaggggtgg
    24421 gtcggcaggt cacatttgac tggaacatcg agcagggagt cgcgcaaaac gtctgggccg
    24481 gccccataac ccaggcccag ttgatgtggg cccagatcta ccgcgacaca acgctggaag
    24541 cacgcatcga acttgctgac ggcgctgtcg cggatgtcga tgaggtcgcc cgcgccgtgc
    24601 tggacttgcc ggaagtcagc cgaaaggtcg gcgaagcaga gcctgccaag caagcccctc
    24661 tggcgccttc agaggcctca acaacgtcgg ctcaggacat cggcgcatcc gcaccgactc
    24721 cagcaacgac gttgccagcg cccatcccgg agctgtggca ggcactgctc gacgcagagg
    24781 aagaagtact gccgatggtg acggtctccg gcgagcgacg cgccaatccg gagcgacgcg
    24841 gccaagtgct tgtcccctat cacttggaca gcggcggcat cgactacgac ggcggagaca
    24901 ccgtcgtcgt cgagttcctc gggactgaca agaactggcg ctcttgcggc ttactaagcc
    24961 tgcgggacac gaccttcggc gaccaggcgg aactcgccat tgacgacttc aacagtcgca
    25021 caaacctcaa gatcggcgaa cgtctgcgtc ttcgaagcac gctcgaaaaa gcctcgttta
    25081 cgcgccgcag ccaagccgtg cagcgactgc tgaatgacaa ggcggtcata ccggggctga
    25141 ccagctactt caatcccgcc gtttccgctg aaagggttcc cctcagctat ccggttccga
    25201 cggacgccga tctggacgtc tacgccgacg gggacaagca gctcaacgag agccaacgcg
    25261 acgccttccg caaagtgctt ggcaacggcc ccatcagcct tctgcaaggg cccccgggca
    25321 ccggtaagac ctggtttatc gcctcgctgc tgcactacct gatgaccaag cagcagtcgc
    25381 gccgcatcct gctggtcagc cagtctcacg aggcagtcaa caacgctctc gagaaagcgc
    25441 tcgatctgtg tcgcgacaag ggcatcccgt tcgacgccgt tcggctgggc aacgagtccg
    25501 ctgcgtccga ccgaattcgc caccttcaca gcgggtccat cgagcaagcc taccgcgaga
    25561 gattcaaggc ggagcagcga ctgcgcatca cgcggcttgc ggtagagatg ggcctgcccg
    25621 aggactttgc tcgcgagttc gtcgaacttc accatcggct cggtggcctt gctacgcgga
    25681 tcggcttcat ggctggcgaa gtcgagtcgg cgcccgaagg cgctgagcga agcgctgcaa
    25741 tggcccgcct caagtcgttg ctgggtagtt tcgacgacat ctgccaggac gtctatgacg
    25801 agtccgttga cggacctccg gcgaccgtca ttgccaccct cgaggcgcgg ctcattgccg
    25861 agcacggcgt agcctcgccc gatgcggttc accgcctgcg aaggctggtg cgcctttcgg
    25921 aggactggct atccgcgctg ggctcgccgc aggccaactt ctcggagttc ctggccaagt
    25981 ctcgaactgt cgtggccggc accctggtcg gcgtcggtca ccgggcgtcc ggggtagtgc
    26041 agaacctgta cgactgggtc atcatcgacg aggcgggccg tgccgccccc agcgagcttg
    26101 cggtcgcgat gcagactggg caccgcatct tgctggtcgg cgaccacaag caattgccgc
    26161 caacgttctc gcaagaagtc agagaagcca tcaaggcgcg ctatgccgtc agcgacgagt
    26221 catccgtctt cggcagcgac ttcgaacgca tcttcgagtc cgactacggt cggcgcgtcg
    26281 gcgcgtcact gctgcgccag taccgcatgg cgccgaacat cggcgagctg gtgtcctcct
    26341 gcttctacga cggccgactg gagacgggcc gcggcgaccc ccccgagttc tattcgcacc
    26401 tccccaacct gctcaggcac caggtgaatt gggtcgacac caccccgctc ggcgcacacg
    26461 cgcacgagca ggtgtcaagc gccggcgacc agcggtggaa cgagcttgaa gccaaggtcg
    26521 tgatgggtgt gcttcggcaa atcgtggaga gccacgaatt catggatcga ctcacgcagg
    26581 agcttgaacc cggggagccc gccgtcggca tcatctgcat gtacagccgc cagcgcgaag
    26641 tcctagaccg gatgaaggcg gaggccagct ggctcggcga cgcgcgcaag ctcgtcaaga
    26701 ttgacacggt cgacagctac cagggcaagg aaaaccgggt ggtcatcctc tctaccgtgc
    26761 gcaacaacga ccgtggcaag gtcgggttcc tgaccagtcc gaaccgcatc aacgttgcct
    26821 tgtcgcgcgc catggacagg ctatttgtcg tgggctccag cgcgatgtgg aaccgaaaga
    26881 acgccgacct cccactcggc cgtgttctgt cgaaggttca gcagatgagc agtgaaggca
    26941 aggcgatcac gctatccgcc aaggagttcc tgcaatgagc ggcgccagct acgaattcaa
    27001 tcgggccacc ttcgggctgc ctgtcgaaag ctatcgggtg gattccttca tcaccaaggg
    27061 cgagcgcctt ccagtggtga cggagttcgt cctacgtctg ctccgaatct gcggtcccat
    27121 caccgttccg gcttttcgcg actacttcgg cttctccgag gccgagtctc tgtcggtggt
    27181 gaaccgcccc gggtttcgtg gaggctggtt ggtttaagtc agacctccac tg
//
LOCUS       DAOMFD010000007        26986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_266_length_26986_cov_32.952174, whole genome shotgun sequence.
ACCESSION   DAOMFD010000007 DAOMFD010000000
VERSION     DAOMFD010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 26986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26986
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_266_length_26986_cov_32.952174"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="PL152_00875"
     CDS             complement(<1..1012)
                     /locus_tag="PL152_00875"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HQR47852.1"
                     /translation="MSAVTSHVHQKTSSAPPGAAAAAALSAQELAIEQGAKALHGNIS
                     ERVLRIFDAIRGYGSPRVTVHRAVYFTESFKQTEGQPLVLRWAKALKHVAENIPVTIF
                     EDELIVGRPNTWLGRYGLVYGELDGSLLKAAAEAADKQVGQKGAVAFTAEDKRAIEDV
                     LYPYWNGKDFGTSFVRRLPEDTRFFFFGPDRNNASNATGVGISTGLWRHSQNWAHDFA
                     KIINKGCAGIRAEAEARLAALEDPYDVVEKKPFLEAVIITCDAMVLWARRYSALAAEL
                     AAREADPHRKRELEEIAANCARVPEHPARTFHEALQAQWFAQIFSRLEQNIGGQVSQG
                     RMD"
     gene            1261..1800
                     /locus_tag="PL152_00880"
     CDS             1261..1800
                     /locus_tag="PL152_00880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47853.1"
                     /translation="MRSATALAYIILLLPITADAALTQVASDGAIIEHHFQVSATPTV
                     AWNALVHPELWWPSDHTWSGDRANLRLTAQAGGCFCENWGESSAEHGYVVMSVPGKVL
                     RIRGALGPLQDMAVTAVLTIRLAAAASGSTEATVTYRISGDASHKLDALAPAVDKVVG
                     LQFGNFAAYASGRPVKPAP"
     gene            complement(1784..3622)
                     /locus_tag="PL152_00885"
     CDS             complement(1784..3622)
                     /locus_tag="PL152_00885"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQR47854.1"
                     /translation="MKRVLKVLGWTALVLLVVGAYAGYRIVWGHPFTINQLANRQAFF
                     FLVRNPELFTSVGIADGTIVDFHSGKLADVGVAKRDSDYDFAAKSIEEVKQFDRARLD
                     RQDQITYDILLDLYGSQLDFRPFDWLSSEGIYPISPMFGTQVTLVGFMQTQHVVKNDK
                     TARNYVSRLRAMGPKLDALTAEMRRQSDAGVVMPPSLIAKSIAVIDDTLAPKPADNAL
                     VTTFVERMKAAKDIDAATQSQLRDEAVAAVTNDVYPAFRRMRDALVAEQPKAAGTAAG
                     VGRLRDGAAYYQAMLKQFTTTDYTPAQIHQIGLDEVARILPEMNALLVSQGFTQGTVA
                     ERMKALGEDPRYLEPDTDEGRAKLLARYREILAEVAKLMPQYFRTIPPGELKVEREPP
                     SVEKGSSGAHYNPPAMDGSRPGTFFANLRDTKETPTWGMKTLAYHEGIPGHHFQIATA
                     LGLKDLPLIRQQSIYGAYVEGWALYAERLAAEVGLYKDDPLGDLGRLQAELLRAVRLV
                     VDTGLHADGWTREQAIDYMVNTTGMAESDATSEVERYMALPGQACSYKVGQLKILELR
                     EKARRELGPKFDLKDFHTVVLENGSVPLTVLERLVDEWIAKVKAQA"
     gene            3760..6453
                     /locus_tag="PL152_00890"
     CDS             3760..6453
                     /locus_tag="PL152_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47855.1"
                     /translation="MSARIAEQRRLDECANAGVPWKRWGPYLSERQWGTVREDYSDNG
                     NAWDYFPHDQARSRAYRWGEDGLGGVSDDEQRLCFALALWNGRDPILKERLYGLTNSE
                     GNHGEDVKEYYFYLDSTPTHSYMKFLYKYPQAEFPYAKLIEENKARGRAGQEYELIDT
                     GVFDDDRYFDVFVEYAKAAPDDILVRITACNRGPDVATLHLLPTLWFRNTWSWGQEVE
                     RPSLTAAGERVIVARHAELGRQELVCGGQPALLFTENETNHARLWGQANASPWTKDAF
                     HEYVIHGDIAAVNPARTGTKAAALYVLEVPARGEKVVQLRLSPRLALADGPDFDRVFK
                     DRLAEADRFYDVITPDRLSADEHRVMRQALAGMLWSKQYYYFDLDRWLSEHGSNPVRG
                     AFVRSTRNVDWFHMRNSDVISMPDKWEYPWYAAWDLAFHTLALSMVDIDFAKQQLQLM
                     LREVYLHPSGQIPAYEWNFGDVNPPVHAWATLFVYSADRGKSGRGDLDFLRHSFQMLL
                     FNFNWWLNRKDPTGRNVFTGGFLGLDNIGVFDRSAPLPTGGHLEQADGTAWMAFYCQN
                     MLEIALELAADDPDYEDLAYKFAEHFLWIAHAMDRIGEHADEMWDEADGFFYDVLRLP
                     DGRAGRLKVRSMVGLLPLCATTVFPADSAERFPKLMKRLGEFLERNPDLRRLLNPRER
                     SPQGRPMLALLDEQKLRRVLARMLDEAEFLAPGGIRSLSRHHLDHPFGVQLDGNRYEV
                     RYLPAESDSGMFGGNSNWRGPVWMPVNALIIRALLQFYAYYGDAFRVEYPTGSGRELT
                     LFEVAHQLSDRLAGIFLRGLDGRRPVYGGTEKFQADPHWRDLILFYEYFHGDNSAGIG
                     ASHQTGWTGVVARFMQLFGHLEARDLVNAANLRPLARYYRE"
     gene            6491..7558
                     /locus_tag="PL152_00895"
     CDS             6491..7558
                     /locus_tag="PL152_00895"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR47856.1"
                     /translation="MIVKNEAPVIRRCLDSVRPLIDYWVIVDTGSTDGTQQIIREHMK
                     DVPGELHERPWQDFAHNRSEALELARPHADYSLIIDADDVIEIPRGYTLPRLKADAYY
                     LDIDHPPVRYQRMLVVSNARPWRYRGVLHEFLTCEGAGPAGKLPLLYRMNFDGARRRD
                     PSTFEKDARVLEQALSTETDPFLKSRYTFYLAQSCRDSGDKRRALDLYLARAGQGFWQ
                     DEVYVSLLEAARLMEQLDYPEQQVIDAFLEAAKAAPRRAEALHGAARYCRIKQRYEEG
                     YRYAKSGLKLQQPSDGLFVEASVYQWSLLDEYSILCFWVGRYRDSLEACLTLLTGEHC
                     PEEQRERILANGAQAKQKLTG"
     gene            7654..8079
                     /locus_tag="PL152_00900"
     CDS             7654..8079
                     /locus_tag="PL152_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQR47857.1"
                     /translation="MPTNAQFLMPVLALVAWTFVMWAWMYATRIPAMRAAGIDPQDAA
                     YSDALGERLPPQVRWKADNYNHLHEQPTIFYALMFFAALTGGGDAIALELAWAYVALR
                     VVHSLVQATVNRIVVRFALFMLASLVLLAFTVREAMRLL"
     gene            complement(8083..9651)
                     /gene="nadB"
                     /locus_tag="PL152_00905"
     CDS             complement(8083..9651)
                     /gene="nadB"
                     /locus_tag="PL152_00905"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR00551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HQR47858.1"
                     /translation="MSPAIHSDVLILGCGIAGGTAALELADAGLDVVIVTRAAEAADS
                     NTYWAQGGIIFRGEGDSPELLVHDVLNAGAGICREQAVETLARTGPDLVQSVLIDRLK
                     VPFDRRPDGSLSLGREGGHSLPRIVHSTDATGRAIEDALIAELRRHPHVRFLTAHTAV
                     DLLTPAHHGRDRRAVYDPVSCVGAYVLDQGSGNVVRCFARSTILATGGLGQIYLRTTN
                     PVGSRGDGLAMAYRAGARVINTEYVQFHPTAFYHRNAPCFLVTEAVRGAGARLVDERR
                     RPFMDRYDPEWKDLAPRDVVSRSIHAEMLSKGVKNVYLDLASYIPAERIRAEFPGMFQ
                     SCLEYGIDITRDLLPVVPAAHYSCGGVWVDANGRSTLDRLYAIGEVSCTGVHGANRLA
                     STSLLEGLVWGHRSAQDIVASLEPRGATLYDDIPPWEPTGAVAPDPALVAQDVTSIRH
                     IMWNYVGLVRTTRRLARAIRDLRNLEVEIERFYRAAALSDDLIGLRNSIRVALIVTLA
                     AWENRESRGCHYRE"
     gene            complement(9648..10700)
                     /gene="nadA"
                     /locus_tag="PL152_00910"
     CDS             complement(9648..10700)
                     /gene="nadA"
                     /locus_tag="PL152_00910"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF006879.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HQR47859.1"
                     /translation="MQAEQIYRDLKAKLGDVLPDAEILLKAEVAAEVQRLKVERDAVI
                     LGHNYMEPALFHSVPDFQGDSLELSRRAAETDKSVIVFCGVRFMAETAKILNPKKTVL
                     LPTERGGCSLAESITAADVRALRQAYPGVPIVTYINTYADVKAECDICCTSSNAVAVV
                     ESLKSDTVIFLPDEFLARNVARETGRHVILPSVLPKSGSGASTDVEQIMVAWKGRCEV
                     HELFTPRDVDDVRKQFPDVMILAHPECSPDVVEKADFSGSTTAMIKAVEKSKAKRFLL
                     LTECSMGDNIVAANPGKEMLRLCSHRCPHMHEITLEHTRDSLRDMRWVIDVPEDIRVR
                     ALRAVERMLAVGPKVD"
     gene            10774..12015
                     /locus_tag="PL152_00915"
     CDS             10774..12015
                     /locus_tag="PL152_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HQR47860.1"
                     /translation="MTAASRTHDVVVFGATSFVGQILSRYLVDEYGGRSGLRWAIAGR
                     SQAKLEELRDALGPAAGKLPIIVADAADDRALRSMCEGTRVVVSTVGPYALYGEPLVR
                     ACVETATDYCDLSGEPQWIRRMIDTYEAAARRSGARIVHCCGFDSIPFDLGVFFLQRE
                     ARARFGKPCSRVKMRVKGMSGGMSGGTAASMMNAFKELAASPELRKQLANPYSLCPKG
                     YGSKVRQPNVKFAEYDRDFGGWVAPFVMAALNVRIVHRTNALSRNAYGTDFRYDEAVF
                     TGRGLRGRAFAAAIASGIGGLAVAASIGPLRSALQRFVLPSPGTGPSPKQQKHGYFDL
                     RFLGQTDDGRVIRAKVTGQGDPGYGSTSKMLGQAAACLARDVPHSRVSGGFWTPATIF
                     GDALVERLEKHSGMRFKVLED"
     gene            12082..13626
                     /locus_tag="PL152_00920"
     CDS             12082..13626
                     /locus_tag="PL152_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47861.1"
                     /translation="MHRHLCAALAAFALIATGSAHAADESKPADGDKKAEADKSAEPA
                     SSVTQHQVTIGGQVVKYTATAGWLIMKDDKDKPIARFGYTAYTRDGVKDLSKRPVMFA
                     YNGGPGSSSIWLHMGILGPRRVVVNDQGYAPPPPSVSVDNAYSPLDLTDIVMIDPVGT
                     GFSKAVGDAKPADFWGVDQDIKSVGQFIKRYVSENGRWASPKYLLGESYGGMRSAGLA
                     WYLQSTQNMDLNGVVLVSPFLNAGSGIDGEEIDLPHVLYLPTLAATAWYHDAIPNKPA
                     SLPAYLDEVERFAYNEYAPALMRGYMIPDGEKKSIAAKLAAYTGTTADYWEKADLRVS
                     HPQFLQELMRNQRLVAGRIDSRFVGPAVNPLSETQDYDPFFPAVGPAYTAAFLDYLHS
                     DLKFGKDENYVVTAFDIKWDWQHKRPGEGGWMSPVPNTVPDLAMAMTFNPGLHVLVLQ
                     GYYDLATPWLATRNDLAHLHITPEARKRITVDYFDAGHMMYVHEPAMQKFRDDLAGFI
                     RQTDRL"
     gene            13665..15368
                     /locus_tag="PL152_00925"
     CDS             13665..15368
                     /locus_tag="PL152_00925"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR47862.1"
                     /translation="MTSKSSRPLRRFWGWGLETDALTPMEVGIVKGTMARLGCATQEL
                     PTPPRKEDYRLRAPRVAPPESLADAFSCTPHDRLSHAYGKSFADSVRMWKRDVPQPPD
                     WVAFPADEQAVADILDWATRENVAVVPYGGGSSVCGGVEPAVGPGYSATLSLDLERLN
                     RVLEVERTSRSARIQAGILGPELEDALRPHGLTLRHYPQSFQFSTLGGWIVTRSGGHY
                     ATLYTHIDEFVQAVRMLAPNGPIETRRLPGSGAGPDPNRLVSGSEGVLGVVTEAWMRL
                     QDRPRFRASASVRFAELTAAVRCVRALAQSGLNPTNCRLLDPAEVAWTGVGDGRSTML
                     VLGFESADHALDAWMQRALELVRDCGGEYDAAAVERSLRAGAATEGAEHRKGAAGAWR
                     DAFIRMPYWRDPMVGAGAILDTFETAITWDRFDEFYRGVRADLESAILRATSGGGHVS
                     CRFTHVYPDGPAPYFTYAALGSATGDLASSFAAWRDIKHAANESVTSRGGTITHHHAV
                     GRDHRSGYEREAPALYREALAAAKSRLDPRGILNPGVLIDPLGRDIGVRGALEPRPRA
                     S"
     gene            15428..18628
                     /locus_tag="PL152_00930"
     CDS             15428..18628
                     /locus_tag="PL152_00930"
                     /inference="COORDINATES: protein motif:HMM:NF027232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47863.1"
                     /translation="MHFLTRQAAALAAMLTIATASAATAPDVTSALEWRLVGPHRAGW
                     VTATGGAGNGSDTFYFGAAGGGVWKSDDAGYTWTAIFDGVGSASVGALAVAPSNSEVI
                     YVGMGQVTSRYDIGAGDGVYRSDDAGRTWRHVGLATSRHIGRILVDPRQPDTAWVAAL
                     GSTFGPGGERGVYRTTDGGKTWQRTLVVSEDTGAVDLALDPADSRVVYASAWQVRYRP
                     WLNYFTPDVGPESGLYKSTDGGATWKRIEGGGWPAGPLGRIGLATSARDQGTRVWAVV
                     DAESVGGLYRSDDGGANWAKVNDDPEFVNSYFAQLTALPGDPDAVYVMGRSIHRCSDG
                     GTKCEIVKGAPGGDDYHYLWLDPQRPERMITGADQGAAITLNGGRTWTDWYNQPTGQF
                     YKLSTDDSFPYRIYAGQQDNGTVRIASRSDYGSINWRDWESVGADERDYEIPDPRDPD
                     IVYGSGLGGRLSRWNARNGEVQNVTPWPISSYGERPTDFQQRYSWITPIAMSKLAPYP
                     LYFGSQYLWRTTDRGEHWEKVSPDLSARDPAATGCSGSLDPARARACGYGVIWSIGLS
                     PRDNSEIWVGTDDGVVRLTRNGGRTWTNVTPKGVPAWAKIATVEPSPTTPGSAYIAVD
                     NHRQDDFRPYVYRTRDYGATWTQVTDGLPAGHFVGVVRADTVRAGLLYAGTETGVFVS
                     LDDGAHWRSLQRNLPVAWVRDIEQHGNDLIVATQGRAIWVLDDVSPLRQHDAAAATAR
                     EHLFRPATTVRLRKNQNRDTPLPKEVPAGRNPPTGAVVDYWLAAPAKTVELEFRDASG
                     TVVRRFSSSEPAPTLQGERYFAADWLAPDAPPETSAGMHRFTWNLRYPRPRSTEYEYS
                     ISTAYGAGVALVPQGPVAAPGEYRVVLRVDGREQSAPLKVVMDPRVPVDTASLAAALS
                     LSREIGTLLGKHYAGAAELEYVTQHTGDLRKEQSGDANVIAALDAFDKHVEPIATGSG
                     DLPSDLNLHSIGQVLRSIATDVESSDRAPTEPQRRAVAESAHRLDRALAVWNGVRDTE
                     LPRLNAALAASGLPTITIPAVDQIKLGGPSASREMP"
     gene            complement(18641..19597)
                     /locus_tag="PL152_00935"
     CDS             complement(18641..19597)
                     /locus_tag="PL152_00935"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HQR47864.1"
                     /translation="MPEASPQLSDIRAAAGRIAPYVARTPVMTSRTLDAFTGARLYFK
                     CENFQRIGAFKARGAHNAVLSLPPGVLPQGVVTHSSGNHAAALALAARNAGTHAYVAM
                     PHNAPKAKVESVRRLGGEIVFCEPTVPAREACCAGIRERTGAALVHPYDDWRVIAGQG
                     TAALELLEDAPELEALVAPIGGGGLVSGTAIVGAAHGVATFGAEPAAADDAYRSFVAG
                     SIQPVLRTDTVADGLRTSLSERTFQVIRRHVEDVLTVSETEIVAAMRLVWEVMKIVIE
                     PSSAVPVAALLGAGSRFAGKRVGVILSGGNVDLDALPWSARA"
     gene            19690..21540
                     /locus_tag="PL152_00940"
     CDS             19690..21540
                     /locus_tag="PL152_00940"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQR47865.1"
                     /translation="MTRHGPGKDRLDWGFRALLVVACLVLYLAGSSGTSVFDSDEARF
                     ALAVKEMSARGDWVVPSNFGEPRYNKPILSYWLALASTRLLGMNEFALRLPSALCCIF
                     AVLVTMAVAQRTLSRRAAHYAGIAVATSLYVVIEARSLTADASLLAATTASFWAWSRL
                     RERPPDPLRWQVLLWSSVGFGLLAKVVNVAFIGAAACALTLIETSWSGRTRVAIAVAV
                     LAGAIATTLPRCGWIGPSVLGVLATIFLLHSLLDASARAEWRRLGAAWGIPLAALMFL
                     AWGLPAALATHGGFVAQGLGHHLLARTATPFEGHSGWPGYYLVATFVAFFPWASFLPA
                     AVTRAWCERRDPWLAFLLAWTLGPLVLVELTTSKLPHYLLVAFPAISLLVAMLIDGRS
                     DGNRSWTRRERVVEAAVFAALCGCVAVASGYVAASFGSASVRAPAAITAAVAIAVAAA
                     VVRSWLHEPPRRLLARLAGGVVILYLAVFAVLLPALEPLRLAPQLGAAVAQRLEPGER
                     LVLCRIREASVGFYLPRVPDATGDLGTVTLQLRSSPRDALVLVPDDERGLPARLQAGE
                     QDRWERLEAVDGIVLPELSLRRMLVLRRHGNAGVELPRLAAETQSRVSRP"
     gene            complement(21516..22169)
                     /locus_tag="PL152_00945"
     CDS             complement(21516..22169)
                     /locus_tag="PL152_00945"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HQR47866.1"
                     /translation="MTTRHATLRKEIVTVACELDAAGMMPNKSGNLSCRAPGGFVITP
                     SAVAYRELEPSLLVELHDDGTVGAGDLRPSSEWLMHAAIYGQRPDVAAIVHTHSPSAT
                     ALSCAGVGIPAFHYMIALAGGEVRCMPYATFGSAELAANAVLGLEGRRAVLLANHGVV
                     AVGPSLAAAKAVALEVENMASQYLALRAAGMEPVVLDDEELQKVIEKFTDYGRLTRD"
     gene            complement(22159..23289)
                     /gene="mtnA"
                     /locus_tag="PL152_00950"
     CDS             complement(22159..23289)
                     /gene="mtnA"
                     /locus_tag="PL152_00950"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HQR47867.1"
                     /translation="MRVHGEHWRSIFVADDGWTVTVIDQTLLPFEFEVCHLRTVEDAA
                     RAIRDMIVRGAPLIGVTAAHGVALAMRSDASDASLQSALALLGAARPTAVNLRWALGR
                     MAEALQPLPIAQRVQHAYAEAGRIAEDDVAACAAIGRHGATIIEQLSAGAGRRPVNIL
                     THCNAGWLACVDWGTALAPVYAAHEAGIAVHVLVDETRPRNQGGSLTAFELAGHGIPH
                     EVIVDNAGGHLMQRGRVDLCIVGSDRVTARGDVTNKIGTYLKALAAHDNRVPFYAALP
                     YSTIDWQIDDGVREVPIEERSSREVTHVRGRTNGGQVLEVEVVRPGSAVANPGFDVTP
                     ARLVTGIITERGIAAASRAGLQSLYPEAASRADRSTGERDDH"
     gene            complement(23291..24169)
                     /locus_tag="PL152_00955"
     CDS             complement(23291..24169)
                     /locus_tag="PL152_00955"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: protein motif:HMM:NF006492.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HQR47868.1"
                     /translation="MTKAVLGIIGGSGIYDLPGLEGLSERRVSTPWGEPSDALRFGRV
                     GGTEVVFLPRHGRGHRYSPSDINYRANVDAMKRAGVTDLVSFSACGSFREELSPGTFV
                     LVDNFVDRTVRRANSFFGAGCVAHVPFAHPVGPALQELVARAADGEGIPFHRGGTAVC
                     MEGPQFSTLAESLSYRDAGYDVIHMTSMPEAKLAREAEITYAVVCMVTDYDCWHPTHD
                     SVEVSTLLKVMDENRGKAQRLFARLARDFPAEHVSCPVRSDRALEGAILTRPEARDPA
                     LLAKLDAVAGRVLGGH"
     gene            complement(24256..25353)
                     /locus_tag="PL152_00960"
     CDS             complement(24256..25353)
                     /locus_tag="PL152_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR47869.1"
                     /translation="MSTGRYFLYSMQLSWYSAKVRPYLRFRRLPFDERTPTAREYYGE
                     IKRRCGHAVVPIVITPDGEWLQDSSVIIERLEERFPGYPIVPTSPRQHFAAYLLELWA
                     DEFWLSAGVHARWSFPENYPKWRDELATAFAPGFPTFVQRLLPLPLKWFMQRVNTQLG
                     ATPDQVALIERWMAQQLDHLERHFALMPYVFGARPSLADFSLAGPICGHLRYDESASR
                     RLLRPRPRLYAWSERMMSATPAPSDGPYLADDAIPESLVPLLDSAFHEMVPMLAALAE
                     LVRPMKNGRGGHLPRIVGKVTYPYAGERFTRHGAPYMLWMAQRLLEVVAAMPADQAAE
                     VRRWVASLGGQGLLDLDIPRVTRVGVHAALA"
     gene            25483..26670
                     /locus_tag="PL152_00965"
     CDS             25483..26670
                     /locus_tag="PL152_00965"
                     /inference="COORDINATES: protein motif:HMM:NF012588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase"
                     /protein_id="HQR47870.1"
                     /translation="MATADRDRIPRDADADADYTKQAAARRAEFLREKTGATLSHVQG
                     YSFDPGVLPGNIENFTGVAQVPIGIAGPLRVNGEHAQGDFYVPMATTEGTLVASYNRG
                     MRVLAESGGVTTTVVKRSMQRAPVFMFTTAREARDFGDWLRTNHEGVRDAAQSTTRSG
                     RLVEIEQYPLANMLYLRFNYTTGDAAGQNMTGKATRAACEWIRESHPAHPKYMLSGSI
                     DTDKKHSAINTLRTRGRRVVAEAVIRKDVLQRLMRVDTQTLFRARQVSNAGSMLAGSA
                     YNGPHAANGITAMFIACGQDVANVAESHAGITYAQLLDNGDYYWSVTLPSLIVASYGG
                     GTGLPTQRECLEVLGCYGTGKADKLAEIVAGVVLAGDISLGCAVLADEWVTAHEQLGR
                     NRP"
BASE COUNT         4176 a         9359 c         9215 g         4236 t
ORIGIN      
        1 ggtccatgcg gccctggctc acctggccgc cgatgttctg ctcgaggcgc gagaagatct
       61 gcgcgaacca ctgcgcctgc agggcctcgt ggaaagttcg cgccgggtgc tctggcacgc
      121 gcgcgcagtt ggctgcgatt tcctccagct cgcgtttgcg gtgcgggtcg gcctcgcgcg
      181 cagccagctc ggcggcgagc gcggagtagc gacgcgccca gaggaccatc gcatcacacg
      241 tgatgatgac cgcctcgagg aacggcttct tctcgacgac gtcgtaaggg tcctcgagcg
      301 ccgctaggcg ggcttccgcc tccgcccgga tgccggcaca gcccttgttg atgatcttgg
      361 cgaagtcgtg ggcccagttc tgagagtgcc gccagagccc cgtgcttatg ccaacgccag
      421 tggcgttcga cgcgttgttg cggtccgggc cgaaaaagaa gaaccgggtg tcctccggca
      481 gcctgcggac gaaactcgtg ccgaagtcct tgccattcca gtatggatac aggacgtcct
      541 cgatggctcg cttgtcttcg gccgtaaacg cgacggcacc cttctggccc acctgcttat
      601 ccgcggcctc ggccgccgcc ttgagcaggc tgccatcgag ttcgccgtag acgagtccat
      661 agcggcccag ccacgtattc gggcgaccga caatgagttc gtcctcgaag atcgtgaccg
      721 ggatgttctc cgcgacgtgc ttcagcgcct tggcccagcg cagcaccagc ggctggcctt
      781 ccgtctgctt gaaggactcc gtgaagtaca cggcgcggtg gaccgtcact cgaggactgc
      841 cgtaaccgcg gatggcatca aagatccgca gcacccgctc gctgatgttg ccatgcagcg
      901 ccttcgcgcc ctgctcgatc gcgagttcct gggccgacag ggctgcggcg gccgcggcac
      961 ccggcggtgc ggacgaagtc ttctgatgca catgcgatgt cacggcgctc atcctgcgta
     1021 ctccttcaga tcggtgcccg acacgggcgg acgcgtcccc cacgaaatcg accatacgcg
     1081 ccctctcgta aaggccttgc gcctttcgtt gcgccttttg agcatttggt ctccaatgat
     1141 tcactggcga tgccgctggg ccgacgcgcg ggacgcagca tcgctgcaat gcagcaagca
     1201 gacgcggcgc agaccttcgt gcaacgatag gtgggtttct gcaaccaggg ggacatcgcc
     1261 gtgaggtcag ccaccgcgct tgcgtacatc atcctgctgc tgccgatcac ggcggatgcc
     1321 gcgctgacac aggtcgcatc cgacggcgcg atcatcgaac accacttcca ggtcagcgcg
     1381 acaccgacgg tcgcgtggaa tgcactcgtg caccccgaac tttggtggcc gtccgaccac
     1441 acgtggtccg gcgaccgcgc gaacctgcgc ctcacggctc aggccggcgg ctgtttctgc
     1501 gagaactggg gagaatcgag cgccgagcac gggtatgtcg tcatgtccgt gcccggcaag
     1561 gtgctgcgga tccgcggcgc actgggtcca ttgcaggaca tggctgtcac cgcggtactg
     1621 acaatccggc tggcagcggc agcgtctggc agcaccgaag cgaccgtgac ctaccgcatc
     1681 agcggcgacg cctcgcacaa gctcgacgcc ctggccccgg cggtggacaa ggtcgtcggc
     1741 ctgcagttcg gcaacttcgc ggcctacgcc tccgggaggc cggtcaagcc tgcgccttga
     1801 ccttcgcgat ccactcgtcg accaggcgct cgagcacggt gagcggcacc gagccattct
     1861 cgagcacgac cgtgtggaag tccttcaggt cgaatttcgg ccccagctcg cgacgcgcct
     1921 tctcgcgcag ttcgaggatc ttgagctgcc cgaccttgta cgagcacgcc tgccccggca
     1981 gcgccatgta gcgctcgacc tccgaggtcg cgtcgctctc cgccatgccg gtggtgttca
     2041 ccatgtagtc gatcgcctgc tcgcgcgtcc agccgtcggc gtgaaggccg gtgtccacca
     2101 cgagacgcac ggctcgcaac agttccgcct gcaggcgccc gaggtctccg agcggatcgt
     2161 ccttgtacag gccgacctcg gccgcgagcc gctcggcata cagcgcccag ccttcgacgt
     2221 aggccccgta gatcgactgt tgccggatca gcggcaggtc cttgaggccc agcgcggttg
     2281 cgatctggaa atggtggccc gggatgccct cgtggtacgc gagggtcttc atgccccagg
     2341 tcggcgtctc cttggtgtcg cgcaggttgg cgaagaacgt gcccggacgc gaaccgtcca
     2401 tcgccggggg attgtagtgc gccccgctcg agcccttctc caccgacggc ggctcgcgct
     2461 ccaccttgag ttcgcccggc gggatggtgc ggaagtactg cggcatgagc ttcgcgacct
     2521 cggcgaggat ctcgcggtag cgcgccagca gcttcgcgcg gccctcgtcg gtgtccggct
     2581 cgaggtagcg cgggtcctcg ccgagcgcct tcatccgctc ggcgacggtg ccctgcgtga
     2641 acccctggct caccagcagc gcattcatct ccggcaggat gcgcgcgacc tcgtcgagcc
     2701 cgatctggtg gatctgcgcc ggcgtgtagt cggtcgtcgt gaactgcttc agcatcgcct
     2761 ggtaatacgc cgcgccgtcc ctcaggcgcc cgacgccagc ggccgtgccc gcggccttcg
     2821 gctgctcggc gaccagcgcg tcgcgcatgc gccggaaggc ggggtaaacg tcgttggtca
     2881 cggcggcgac cgcctcgtcg cgcagctgcg actgcgtggc ggcgtcgatg tccttggccg
     2941 ccttcatgcg ctcgacgaag gtcgtcacca gcgcgttgtc cgccggcttc ggcgcgaggg
     3001 tgtcgtcgat gaccgcgatc gacttggcga tcagcgacgg cggcatcacc acgcccgcgt
     3061 ccgactggcg ccgcatctcg gcggtgagag cgtcgagctt cgggcccatc gcgcggaggc
     3121 gactcacgta attgcgcgct gtcttgtcgt tcttgacgac gtgctgggtc tgcatgaacc
     3181 cgaccagggt cacctgcgtg ccgaacatcg ggctgatcgg atagatgccc tccgacgaca
     3241 gccagtcgaa cggcctgaag tcgagctgcg agccgtacag gtcgagcagg atgtcgtagg
     3301 tgatctggtc ctgccggtcg agcctggcgc ggtcgaactg cttgacctcc tcgatggact
     3361 tcgccgcgaa gtcgtaatcc gagtcgcgct tggcgacgcc gacgtcggcc agcttcccgg
     3421 aatggaagtc gacgatcgtg ccgtcggcga tgccgacgga cgtgaacaac tccggattgc
     3481 ggaccaggaa gaagaatgcc tgccggttcg ccagctggtt gatggtgaac ggatggcccc
     3541 agacgatgcg gtagccggcg tacgccccga cgaccagcag caccagggcg gtccagccga
     3601 ggaccttcag gactcttttc atttgattct ccccgcggta agcgcgcgag gctaccgccc
     3661 gtgcacgccc cccgcaagcg tcccgcccgt tctgcgcgcc gccgtgccgg gcaatgggaa
     3721 taaaatgggc gacgggcaag acgaaccagg acgacgatca tgagtgcacg gatcgccgag
     3781 cagcggcgac tggacgaatg cgccaacgcc ggagtgccgt ggaagcgctg ggggccgtac
     3841 ctcagcgagc gccagtgggg caccgtgcgc gaggactaca gcgacaacgg caacgcctgg
     3901 gactactttc cgcacgacca ggcgcgctcg cgtgcctatc gctggggcga ggacggcctc
     3961 ggcggcgtga gcgacgacga gcagcgcctg tgcttcgcgc tcgcgctctg gaacgggcgc
     4021 gacccgatcc tgaaggagcg tctctacggg ctcaccaaca gcgagggcaa ccacggcgag
     4081 gacgtgaagg agtactactt ctacctcgac agcacgccga cgcactcgta catgaagttc
     4141 ctgtacaagt acccgcaggc ggagttcccc tacgcgaagc tgatcgagga gaacaaagcc
     4201 cgcggccgcg ccggccagga atacgagctc atcgacacgg gcgtcttcga cgacgaccgc
     4261 tatttcgacg tgttcgtcga gtacgcgaag gcggcaccgg acgacatcct ggtgcgcatc
     4321 acggcctgca atcgcggtcc ggacgtcgcg accttgcacc tgttgccgac gctgtggttc
     4381 cgcaacacct ggtcgtgggg ccaggaggtc gagcgtccgt cgctgaccgc cgcgggtgag
     4441 cgcgtcatcg tggcgcgtca cgctgaactt ggccgccagg aactggtgtg cggtggccag
     4501 ccggcactgc tgttcacgga gaacgagacc aatcacgccc ggctctgggg acaggcaaac
     4561 gcgtcgccct ggaccaagga cgcgttccac gagtacgtga tccatggcga catcgcggcc
     4621 gtcaaccccg ccaggaccgg caccaaggcc gccgctctct acgtcctcga agtcccggcc
     4681 cgcggcgaga aagtcgtgca actgcgcctg tcgccgaggc tggccctggc cgacggtccg
     4741 gatttcgatc gcgtcttcaa ggatcgcctg gccgaagcgg accggttcta tgacgtcatc
     4801 acgccggacc ggctgtcggc ggacgagcac cgggtgatgc gccaggcgct cgcgggcatg
     4861 ctctggtcca agcagtacta ctacttcgac ctcgaccgct ggctgtccga gcacggctcg
     4921 aacccggtgc gcggggcgtt cgtgcgcagc acgcgcaacg tcgactggtt ccacatgcgc
     4981 aacagcgacg tgatctcgat gccggacaag tgggaatacc cctggtacgc ggcctgggac
     5041 ctcgccttcc acacgctggc gctctcgatg gtggacatcg acttcgccaa gcagcagctc
     5101 cagctgatgc tgcgcgaggt atacctgcac ccgagcggac agatcccggc ctacgagtgg
     5161 aacttcggcg acgtcaatcc gccggtgcac gcctgggcca cgctgttcgt gtacagcgcc
     5221 gaccgtggca agtcgggccg cggcgacctg gacttcctgc gccacagctt ccagatgctg
     5281 ctgttcaact tcaactggtg gctgaaccgc aaggatccca ccgggcgcaa cgtgttcacc
     5341 ggcgggttcc tcggcctcga caacatcggc gtgttcgacc gcagtgcgcc cttgcccacc
     5401 ggcgggcacc tcgagcaggc ggacggcacg gcgtggatgg cgttctactg ccagaacatg
     5461 ctggagatcg cgctcgagct cgcggcagac gatccggact acgaggacct cgcctacaag
     5521 tttgccgagc atttcctgtg gatcgcccac gcgatggacc ggatcggcga gcatgccgac
     5581 gagatgtggg acgaggccga cggcttcttc tacgacgtgc tgcggctccc ggacggccgc
     5641 gccggccggc tcaaggtgcg ctcgatggtc gggctgctgc cgctgtgcgc gaccacggtg
     5701 ttcccggcgg actcggccga gcgcttcccc aagctcatga aacggctcgg cgagttcctg
     5761 gagcgcaacc ccgacctgcg ccggctgctc aatccccgcg agcgcagccc gcagggacgg
     5821 ccgatgctcg cgctgctcga cgagcagaaa ctgcggcggg tgctggctcg catgctcgac
     5881 gaggcggaat tcctcgcgcc gggcgggatc cgctcgctgt cgcgccacca tctcgaccat
     5941 cccttcggcg tgcaactcga cggcaaccgg tacgaggtgc gctacctgcc ggccgagtcc
     6001 gacagcggca tgttcggcgg caactcgaac tggcgcggcc cggtgtggat gccggtgaac
     6061 gcgctcatca tccgcgcgct gctgcagttc tacgcctact acggcgacgc gttccgggtc
     6121 gagtacccga cgggttcggg ccgcgaactg acgctgttcg aggtcgcgca ccagctgtcg
     6181 gaccgcctcg ccggcatctt cctgcgcggg ctcgacggca ggcgcccggt gtacggcggg
     6241 accgagaagt tccaggccga cccgcactgg cgcgacctga tcctgttcta cgagtacttc
     6301 cacggtgaca acagcgccgg catcggcgca agccaccaga ccggctggac gggcgtcgtg
     6361 gcgcggttca tgcagctgtt cggtcacctc gaggcccggg acctggtcaa cgccgccaac
     6421 ctgcggccgc tggcccgcta ctaccgggag tgatgaggtc ttgccgcgcc ggacgaccct
     6481 gtgcctcagc atgatcgtga agaacgaggc gccggtcatt cgccggtgcc tcgattccgt
     6541 ccggccgctc atcgactact gggtgatcgt cgacaccggg tcgaccgacg gcacgcagca
     6601 gatcatccgc gaacacatga aggacgtgcc cggcgaactg cacgagcggc cgtggcagga
     6661 cttcgcgcac aatcgttccg aggcgctcga gcttgcacgt ccccacgccg attactcgct
     6721 gatcatcgac gccgacgacg tgatcgagat tcccaggggc tacacgctcc cgcggctcaa
     6781 ggccgacgcc tattacctcg acatcgacca cccgccggtc cgctaccagc gcatgctggt
     6841 cgtgagcaac gcccgcccct ggcgctaccg tggcgtgctg cacgagttcc tgacgtgcga
     6901 aggagcgggt cccgcgggca agctgccgct gctgtaccgc atgaacttcg acggcgcgcg
     6961 gcggcgtgac ccgtcgacgt ttgaaaagga cgcccgggtc ctggaacaag cgctttcgac
     7021 cgagacggac ccgttcctca agtcgcgcta cacgttctac ctcgcgcaga gctgccgcga
     7081 cagcggtgac aagcgcaggg cgctggacct gtacctcgct cgtgccggcc agggcttctg
     7141 gcaggacgag gtgtacgtga gcctgctcga ggcggcgcgc ctcatggaac agctcgacta
     7201 cccggagcag caggtgatcg acgcgtttct cgaggcggcc aaggcggcac cccgccgggc
     7261 ggaggcgctg catggcgccg cacgctactg ccgcatcaag cagaggtacg aggaaggcta
     7321 ccgatacgcc aagtccggcc tcaagttgca gcagccgagc gacgggctgt tcgtggaagc
     7381 gtcggtctac cagtggagcc tgctggacga atactcgatc ctctgcttct gggtcgggcg
     7441 ctatcgcgac tcgctcgagg cctgcctgac gctgctcacg ggcgagcatt gccccgagga
     7501 acagcgcgag cgaatcctcg ccaacggggc ccaggcgaag cagaagctga ccgggtagcc
     7561 tgccggccgg tcacgggccc gccgctggtc gctgcgaccg attcgccggc ctgctagtct
     7621 cctccgctgg ataagcaaga ctcagggggt tccatgccga ccaatgccca gttcctgatg
     7681 cccgtactcg cgctcgtcgc ctggacgttc gtgatgtggg cgtggatgta cgcgacgcgc
     7741 atacccgcca tgcgggccgc cgggatcgat ccccaggacg ccgcgtattc cgacgcgctc
     7801 ggcgaacgcc tgccgccgca ggtgcgctgg aaggccgaca actacaacca cctgcacgaa
     7861 cagccgacga tcttctacgc gctcatgttc ttcgcggcgt tgaccggcgg cggcgacgcg
     7921 atcgcgctcg aactcgcctg ggcctacgtg gcgctgcgcg tcgtgcactc cctcgtgcag
     7981 gcgacggtga accgcatcgt cgtgcgcttc gcgctgttca tgctcgcttc gctcgtgctg
     8041 ctcgcgttca cggtccgcga ggcgatgcgc ctgctgtaac gcctactcgc ggtagtgaca
     8101 gccgcggctc tcgcggttct cccatgcggc gagcgtcacg atgagcgcca cgcggatcga
     8161 gttgcgcagg ccgatcaggt cgtcgctcag cgccgccgcg cgatagaacc gctcgatctc
     8221 gacctcgagg ttgcgcaggt cgcggatcgc gcgcgcgagg cgtcgcgtcg tgcgcacgag
     8281 ccccacgtag ttccacatga tgtgccggat cgacgtcacg tcctgggcaa ccagcgcggg
     8341 gtcgggcgcc acggcgccgg tcggctccca gggcgggatg tcgtcgtaga gcgtcgcgcc
     8401 gcgcggctcg aggctcgcca cgatgtcctg cgccgagcgg tgtccccaga cgagtccctc
     8461 gagcagggac gtgctggcga gccggttcgc accgtgcacg ccggtgcacg acacctcgcc
     8521 gatcgcgtag agccggtcga gcgtgctgcg accgttcgcg tcgacccaca cgccgccgca
     8581 ggagtagtgc gccgccggca cgaccggcag caggtcgcgc gtgatgtcga tcccgtactc
     8641 gaggcagctc tggaacatgc ccgggaactc ggcgcggatg cgctcggcgg ggatatacga
     8701 cgccaaatcc aggtacacgt tcttgacgcc tttcgacagc atctccgcgt ggatgctgcg
     8761 ggatacgacg tcgcgcggcg cgaggtcctt ccactccggg tcgtagcggt ccatgaacgg
     8821 ccggcgccgc tcgtccacca gccgtgcacc cgcgccgcgc actgcctcag tgacgaggaa
     8881 gcacggcgca ttgcggtgat agaaggcggt cgggtggaac tgcacgtact cggtgttgat
     8941 gacgcgcgca cccgcacgat aggccatggc gagtccgtcg ccgcgggatc cgaccgggtt
     9001 ggtggtgcgc aggtagatct gcccgagccc gccggtcgcg aggatcgtgg acctcgcgaa
     9061 gcagcgcacg acgttgccgc tcccctggtc gagcacgtag gcgccgacgc acgacacggg
     9121 atcgtacacg gcgcggcggt cgcgaccgtg gtgagcggga gtgagcaggt ccacggcggt
     9181 gtgcgccgtc aggaatcgca cgtgggggtg acgccgcagc tccgcgatca gcgcatcctc
     9241 gatggctcgc ccggtggcgt cggtggagtg cacgatgcgc ggcagcgagt gccccccctc
     9301 ccggcccagc gacaggctgc cgtccgggcg ccggtcgaaa ggcaccttca ggcggtcgat
     9361 cagcaccgac tgcacgaggt ccgggccggt gcgcgcgagg gtctcgacgg cctgctcgcg
     9421 gcagatgccg gcgccggcgt tgagcacgtc gtgcaccagc agctcggggg aatccccctc
     9481 gccccggaag atgatgccgc cctgcgccca gtaggtgttc gagtcggcgg cctcggcggc
     9541 gcgcgtgacg atcaccacgt cgagcccggc gtcggcgagc tccagcgcgg ccgtgccgcc
     9601 ggcgatgccg cagccgagga tcaggacgtc gctgtggatg gcggggctca atcgaccttc
     9661 ggcccgacgg cgagcatgcg ctccacggca cgcagcgcgc gcacgcggat gtcctccggc
     9721 acgtcgatca cccagcgcat gtcgcgcagc gagtcccgcg tgtgctcgag cgtgatctcg
     9781 tgcatgtgcg ggcagcggtg gctgcacagc cgcagcattt ccttgccggg gttcgcggcg
     9841 acgatgttgt cgcccatcga acactcggtc agcagcagga agcgcttcgc cttcgacttc
     9901 tcgaccgcct tgatcatcgc cgtggtgctg ccggagaagt ccgccttctc gaccacgtcc
     9961 gggctgcatt ccggatgcgc caggatcatc acgtcgggga actgcttgcg gacgtcatcg
    10021 acgtcgcgcg gcgtgaacag ctcgtggacc tcgcagcgcc ccttccacgc gaccatgatc
    10081 tgctcgacgt cggtcgacgc gccggagccg gatttcggca ggaccgacgg gaggatcacg
    10141 tgccggcccg tctcgcgcgc cacgttgcgc gcgaggaatt cgtcgggaag gaagatgaca
    10201 gtgtctgact tgagcgactc gaccacggcg accgcgttgc tcgacgtgca gcagatgtcg
    10261 cactcggcct tcacgtccgc gtacgtattg atgtaggtca cgatcggcac gccggggtac
    10321 gcctgtcgca gggcgcgcac gtcggccgcg gtgatgctct cggcgagcga gcacccgccc
    10381 cgctccgtcg gcaggagcac ggtcttcttc gggttgagga tcttggccgt ctcggccatg
    10441 aaccgcaccc cgcagaacac gatcaccgac ttgtcggtct cggcggcgcg gcggctcagc
    10501 tccagcgagt cgccctggaa gtccggcacc gagtggaaca gcgcgggctc catgtagttg
    10561 tgcccgagga tcacggcgtc gcgctcgacc ttgaggcgct ggacctcggc ggccacctcc
    10621 gccttgagca ggatctcggc gtcgggcagc acgtcgccga gcttggcctt gaggtcgcgg
    10681 tagatctgtt cagcctgcat gcgaggaacc gctggtggat gcaccaggtc gggattctat
    10741 gccatgctgc cgccgtccgc cccggaggcc cccatgaccg cagccagcag gacccacgat
    10801 gtcgtcgtgt tcggtgccac gagtttcgtc gggcagatcc tgtcccggta cctcgtcgac
    10861 gagtacggcg gccgcagcgg cctgcgctgg gcgatcgcgg gccggtcgca ggccaagctc
    10921 gaggaactgc gcgatgcgct ggggcctgcc gctggaaagc tgccaatcat tgtggccgac
    10981 gcggccgacg accgggcgct ccgcagtatg tgcgagggca cgcgggtcgt cgtctcgacg
    11041 gtcggtccgt acgcgctgta cggcgagccg ctggtccgcg cctgcgtcga gacggccacc
    11101 gactactgcg acctgtccgg cgagccacag tggatccggc ggatgatcga cacctacgaa
    11161 gcggccgcgc gccgctccgg cgcgcgcatc gtgcactgct gcggcttcga ctcgatcccg
    11221 ttcgacctcg gcgtcttctt cctgcagcgc gaggcccgcg cgcgctttgg caagccgtgc
    11281 agccgcgtca agatgcgcgt caagggcatg agcggcggga tgtcgggcgg caccgccgcc
    11341 agcatgatga atgcgttcaa ggaactggcg gccagcccgg agctgcgcaa gcagctcgcc
    11401 aacccctact cgctgtgccc gaaggggtat ggttcgaagg tccgccagcc gaacgtgaag
    11461 ttcgccgagt acgaccggga cttcggcggc tgggtcgcgc cgttcgtgat ggccgccttg
    11521 aacgtgagga tcgtccatcg gacgaatgcc ctgtcccgca atgcctacgg cacggacttc
    11581 cgctacgacg aggccgtgtt caccgggcgc gggttacgcg gacgcgcctt tgcggccgcg
    11641 atcgcctcgg gcatcggcgg cctggccgtc gctgcgtcga tcgggccgtt gcggtccgcg
    11701 ttgcagcgct tcgtcctgcc gtcccccggc accgggccga gcccgaagca acagaagcac
    11761 ggctacttcg acctgcggtt cctgggccag acggacgacg gccgggtgat ccgtgcaaag
    11821 gtgaccggcc agggcgatcc cggctacggc tcgacgtcga agatgctcgg gcaggccgcg
    11881 gcctgcctcg ccagggacgt cccccactcg cgggtcagcg gcggattctg gacgccggcg
    11941 accatcttcg gcgacgcact ggtcgagcga ctggagaagc actccggcat gcgtttcaag
    12001 gtgctcgagg actgaccgaa tttgcattcc gcccgggaat cggttgtcct gtgcggcctc
    12061 gaattcaggg ggatcgacac gatgcacagg cacctctgcg ctgcgctcgc cgcgttcgcg
    12121 ctcatcgcca cgggatcggc gcacgccgcg gacgaatcca agccggccga cggcgacaag
    12181 aaagcagagg ccgacaagtc ggccgagccc gcgtcgtcgg tcacgcagca ccaggtcacg
    12241 atcggcgggc aggtcgtcaa gtacacggcc accgccgggt ggctgatcat gaaggacgac
    12301 aaggacaagc cgatcgcgcg cttcggctac acggcgtaca cgcgcgacgg ggtgaaggac
    12361 ctgtcgaagc gcccggtcat gttcgcctac aacggcggcc ccggatcgtc gtcgatctgg
    12421 ctgcacatgg ggattctcgg gccgcgccgc gtcgtggtga atgaccaggg ctatgcgcct
    12481 ccgccacctt cggtcagcgt ggacaacgcc tacagcccgc tcgacctcac cgacatcgtg
    12541 atgatcgacc cggtcggcac gggcttctcg aaggcggtgg gggatgctaa gcccgcggac
    12601 ttctggggcg tggaccagga catcaagtcg gtcgggcagt tcatcaagcg ctacgtctcg
    12661 gagaacggcc gctgggcaag ccccaagtac ctgctcggcg agagctacgg cggcatgcgc
    12721 agcgcgggcc tcgcctggta cctgcagtcg acgcagaaca tggacctcaa cggcgtggtg
    12781 ctggtctcgc cgttcctcaa cgcgggctcg ggcatcgacg gcgaggagat cgacctgccg
    12841 cacgtgctct acctgccgac gctggctgca acggcgtggt atcacgacgc gatcccgaac
    12901 aagccggcct cgctgcccgc ctacctcgac gaggtcgagc gtttcgccta caacgaatac
    12961 gcaccggcgc tgatgcgcgg ctacatgatc ccagacggtg aaaagaagtc catcgcagcg
    13021 aaactcgccg cctacaccgg cacgaccgcg gactactggg agaaggccga cctgcgcgtg
    13081 agccacccgc agttcctgca ggaactgatg cgcaaccagc gactggtcgc gggccgcatc
    13141 gactcgcgct tcgtcggccc ggcggtcaac ccgctgagcg agacgcagga ctacgacccg
    13201 ttcttcccgg ccgtgggtcc ggcgtacacg gccgcgttcc tcgactacct gcactcggac
    13261 ctcaagttcg gcaaggacga gaactacgtg gtgacggcct tcgacatcaa gtgggactgg
    13321 cagcacaagc ggcccggcga gggtggctgg atgtcgccgg tgccgaatac cgtgccggac
    13381 ctcgccatgg cgatgacgtt caatcccggg ctgcacgtgc tggtgctgca gggctactac
    13441 gacctcgcga cgccgtggct cgcaacgcgc aatgacctcg cgcacctgca catcacgccc
    13501 gaggcacgca agcgcatcac ggtggactac ttcgatgcgg ggcacatgat gtacgtgcac
    13561 gagccggcga tgcagaagtt ccgcgacgac ctcgcgggct tcatccggca gacggaccgg
    13621 ctctgatcag cggcgtgtcg ccgaggcctg gccgggatac ctgaatgacc agcaagtcca
    13681 gtcgtccgct gcggcgtttc tggggctggg gtctcgagac cgacgccctc acgccgatgg
    13741 aagtcggcat cgtgaagggc acgatggcgc gcctcggctg cgcgacgcag gaactgccca
    13801 cgccgccgcg caaggaggat taccggctgc gcgcgccgcg agtcgcgccg ccggagagcc
    13861 tcgccgacgc attctcctgc acgccgcacg accggctgtc gcacgcgtac ggcaagtcgt
    13921 tcgcggactc cgtgcgcatg tggaagcgcg atgtgccgca gccaccggac tgggtcgcat
    13981 tccccgcgga cgagcaggcc gttgccgaca tcctcgattg ggcgacccgg gagaacgtcg
    14041 cggtcgttcc ctacggcggc ggatcgagcg tgtgcggcgg cgtcgagcca gccgttgggc
    14101 cgggctattc ggccacgctg tcgctcgacc tcgaacgact gaaccgggtg ctcgaggtgg
    14161 agcgcaccag tcgctcggcg cgcatccagg ccggcatcct cggccccgaa ctcgaggatg
    14221 cgctgcgacc gcacgggctc acgctgcgcc actacccgca gagcttccag ttctcgacgc
    14281 tgggcggctg gatcgtgact cgctcgggcg gtcactatgc gacgctctac acgcacatcg
    14341 acgagttcgt gcaggcggtg cgcatgctgg cgccgaacgg gccgatcgag acgcggcggt
    14401 tgccgggcag tggcgcaggg cccgacccga accgactcgt cagcgggtcg gaaggcgtcc
    14461 tcggcgtcgt caccgaggcg tggatgcgcc tgcaggaccg gccgcgattc cgtgcatcgg
    14521 cgtccgtgcg cttcgccgag ctgacggccg ccgttcgatg cgtgcgcgcg ctggcgcagt
    14581 cgggcctgaa tccgaccaac tgccgtctgc tcgatcccgc cgaagtcgcg tggaccggcg
    14641 tcggtgacgg ccgtagcacc atgctggtcc tcggtttcga atcggccgat cacgccctgg
    14701 acgcatggat gcaacgggcg ctggaactcg tccgggactg cggtggcgag tacgacgcgg
    14761 cggccgtcga gcggtccttg cgcgccggcg cagccacgga aggcgcagag caccgcaaag
    14821 gcgccgccgg cgcgtggcgg gacgcgttca tccgcatgcc gtactggcgc gacccgatgg
    14881 tgggcgcggg cgcgatcctc gacaccttcg agacggccat cacctgggac cgcttcgacg
    14941 agttctaccg cggcgtgcgc gcggatctcg agtccgcgat cctgcgggcc accagcggcg
    15001 gaggccatgt gtcctgccga tttacgcacg tatacccgga cggcccggcg ccctacttca
    15061 cgtacgccgc gctcggcagc gcgacgggcg acctcgcctc ttcgttcgcg gcgtggcgcg
    15121 acatcaagca cgcggccaac gagtccgtca ccagccgcgg cggtacgatc acgcaccacc
    15181 acgccgtggg gcgggaccat cgcagcggtt acgagcggga ggcgccggcg ctctaccgcg
    15241 aggcgctggc cgccgccaag tcgcgcctcg acccgcgagg catcctgaac cccggggtgc
    15301 tgatcgatcc gctcggtcgc gacatcggtg tccggggcgc actggagcca cggccgcggg
    15361 cgtcgtgagg tttcggccaa cccggtacac tgtcgccctg tcgcgcatcg tccccgggaa
    15421 tcgatttatg cattttctga cccgccaggc cgcggcgctc gccgcgatgc tcaccattgc
    15481 caccgcgagc gccgcgacgg cgccggatgt cacttccgcg ctcgaatggc ggctcgtcgg
    15541 cccgcaccgc gcgggctggg tgacggcaac gggtggcgcg ggcaacgggt ccgacacctt
    15601 ttacttcggt gccgccggcg gcggcgtatg gaagtcggac gacgcgggct acacctggac
    15661 cgcgatcttc gatggcgtgg gctcggcgtc ggtcggcgca cttgcggtcg cgccgtcgaa
    15721 ttccgaggtc atctacgtcg gcatgggaca ggtgacgagc cgatacgaca tcggcgccgg
    15781 cgacggcgtc taccgctcgg atgacgccgg caggacctgg cgacacgtcg ggctcgcgac
    15841 cagccgtcac atcgggcgga tcctggtcga tccgcgccag ccggacaccg cgtgggtcgc
    15901 cgcgctcggc tcgacgttcg gcccgggtgg cgagcgaggc gtgtatcgca cgacggacgg
    15961 tggcaagacc tggcagcgca cgctcgtcgt gagcgaagac acgggcgccg tggacctggc
    16021 gctcgacccg gccgattcgc gcgtcgtcta tgcgtcggcg tggcaggtgc gttaccggcc
    16081 ctggctcaac tatttcaccc cggacgtcgg gccggagagc ggcctctaca agtcgacaga
    16141 cggcggcgcg acgtggaagc gcatcgaagg cggcgggtgg cccgcaggcc cgctcggccg
    16201 catcggtctc gcgacgagcg cgcgcgacca gggcacgcgc gtatgggccg tcgtcgatgc
    16261 ggagtcggtc ggcggtttat accgctccga cgatggcggc gcgaactggg cgaaggtgaa
    16321 cgacgacccg gaattcgtga actcgtactt cgcgcagttg accgccctgc cgggcgatcc
    16381 tgacgcggtc tacgtgatgg gacgcagtat ccatcgctgc agtgacggcg ggacgaagtg
    16441 cgagatcgtc aagggcgcgc ccggcggcga cgattaccac tacctgtggc tggatccgca
    16501 gcgccccgag cgcatgatca cgggcgccga ccagggcgcg gcgatcacac tgaacggtgg
    16561 tcgcacctgg accgactggt acaaccagcc gacgggccag ttctacaagc tctcgaccga
    16621 cgactcgttc ccctaccgca tctacgcggg ccagcaggac aacggcacgg tccgcatcgc
    16681 gagccgcagc gactacggct cgatcaactg gcgcgactgg gagtccgttg gcgccgacga
    16741 gcgcgactac gagatccccg acccgcgcga cccggacatc gtctacggct cgggcctggg
    16801 cggacggctg tcgcgctgga atgcgcgaaa cggcgaggtg cagaacgtca cgccgtggcc
    16861 gatctcgagt tacggcgagc ggccgacgga cttccagcag cgctattcct ggatcacgcc
    16921 gatcgcgatg tcgaaactcg cgccgtatcc actgtatttc ggctcgcagt acctctggcg
    16981 cacgaccgat cgcggcgagc attgggaaaa agtgagcccc gacctgtcgg cgcgcgatcc
    17041 ggcggcgacg gggtgctcgg gcagcctcga tccggcccgt gctcgggcct gcggctacgg
    17101 cgtgatctgg agcatcggac tctcgccgcg cgacaactcc gagatctggg tcggcacgga
    17161 tgacggcgtg gtgcgcctca cgcgcaacgg cggcaggacc tggacgaacg tgacgccgaa
    17221 gggcgtcccc gcgtgggcga agatcgcaac cgtcgagccg tcgccgacga ccccgggcag
    17281 cgcctacatc gcggtggaca atcaccgcca ggacgacttc cgcccgtacg tctatcgcac
    17341 gcgggactac ggcgcgacgt ggacccaggt gacggacggc ttgccggccg ggcatttcgt
    17401 gggtgtcgtg cgcgcggaca cggtgcgcgc cggactgctc tatgcgggca ccgagaccgg
    17461 cgtgttcgtc tccctggacg acggcgcgca ctggcgctcg ctgcagcgca acctgcccgt
    17521 cgcgtgggtg cgggacatcg agcagcacgg caacgacctg atcgtcgcga cccagggtcg
    17581 cgcgatctgg gtgctggacg acgtctcccc gctgcggcag cacgatgcag ccgctgcgac
    17641 cgcacgggag cacctcttca ggcccgccac cacggtgcgc ctgcgcaaga accagaatcg
    17701 cgacacgccc ctgcccaagg aagtgcccgc gggccgcaat ccgccgacgg gcgcagtcgt
    17761 cgattactgg ctcgcggcgc ccgcgaagac ggtggaactc gagttccgcg acgcgtcggg
    17821 cacggtggtg cggagattct cgagctccga gccggcaccg acgctgcagg gcgagcgcta
    17881 ttttgcggcg gactggctgg cgccagacgc accgccggaa acttcggccg gcatgcatcg
    17941 tttcacctgg aacctgcgct atccacggcc gcgctcgacc gagtacgagt actcgatttc
    18001 gacggcctac ggcgcgggcg tcgccctcgt accgcaggga ccggtcgcgg cgccgggcga
    18061 gtaccgtgtc gtgctgcgtg tcgatggacg cgagcagtcc gccccgctca aggtcgtgat
    18121 ggatccgcgc gttcccgtgg acaccgccag cctcgcggca gccctgtcgc tgtcccggga
    18181 gatcggcacg ctcctcggca agcactacgc cggcgccgcg gaactcgagt acgtgaccca
    18241 acacacgggc gacctgcgca aggagcagtc gggcgatgcc aacgtgatcg ccgcgctcga
    18301 cgcattcgac aagcacgtcg agccgatcgc gaccgggagc ggggaccttc cgtcggacct
    18361 caacctgcac tccatcggcc aggtcctgcg ctcgatcgcc acggacgtcg aatcgtcgga
    18421 tcgcgcaccc accgagccgc agcgccgcgc cgtcgcggaa tcggcgcaca ggctcgaccg
    18481 cgcgctcgcc gtgtggaacg gcgtgcgcga cacggaactg ccgcgactga atgcggcctt
    18541 ggcggcgtcc ggattgccga cgatcacgat cccggcggtg gaccagatca agctcggcgg
    18601 accgagcgca tcgcgcgaga tgccctgacg ggcctggttc ttacgccctc gcgctccacg
    18661 gcagcgcgtc gaggtcgacg ttgccgccgg agaggatcac gccgacgcgc ttgccggcga
    18721 atcgcgaccc tgcgccgagc agcgcggcga ccggcacggc gctcgaaggc tcgatcacga
    18781 tcttcatgac ctcccacacg aggcgcatgg ccgcgacgat ctcagtttcc gacacggtga
    18841 ggacgtcctc gacgtgccgc cggatcacct ggaaggtccg ttcactcaac gaggtgcgca
    18901 gtccgtcggc gaccgtgtcc gtccgcagca ccggctggat cgagcccgcc acgaacgacc
    18961 ggtaggcgtc gtcggccgcg gcaggctcgg cgccgaatgt cgcgacgccg tgcgcggcgc
    19021 cgacgatcgc ggtgccactg acgagcccgc ccccgccgat cggcgccacc agcgcctcca
    19081 gttccggcgc gtcctcgagc aattcgagcg ccgcggtgcc ctggcccgcg atcacccgcc
    19141 agtcgtcata aggatgcacc agggctgcgc cggtacgttc gcggatcccg gcgcagcagg
    19201 cttcgcgcgc cggcaccgtg ggctcgcaga agacgatctc gcctccgagc ctgcgcaccg
    19261 actcgacctt ggccttcggc gcgttgtgcg gcatcgcgac gtatgcgtgc gtaccggcat
    19321 tgcgcgccgc gagcgccagc gccgccgcgt ggtttcccga cgaatgcgtg acgacgcctt
    19381 gcgggagcac gccgggcggc agcgacagga ccgcattgtg ggcgccccgc gccttgaagg
    19441 cgccgatgcg ctggaagttc tcgcacttga agtacagccg tgcgcccgtg aacgcgtcga
    19501 gggtgcggct ggtcatcacc ggcgtgcgcg cgacgtacgg cgcgatgcgc cccgcggcgg
    19561 cgcggatgtc ggagagttgc gggctcgctt cgggcatcgg gtctcctggg cggccaccgg
    19621 gatcgtactg gaaacccggg cagcgtccag cgggactccc cgtcgccgcg gagcgcgcat
    19681 actggggccg tgactcggca cggacccggc aaggaccggc tcgactgggg cttccgcgcc
    19741 ctgctcgtcg tcgcctgcct ggtgctgtat ctcgccggca gctccggcac cagtgtcttc
    19801 gacagcgacg aagcccgctt cgcgctcgcc gtcaaggaaa tgagcgcgcg cggtgactgg
    19861 gtggtcccga gcaacttcgg ggagccgcgc tacaacaagc cgatcctcag ttattggctg
    19921 gcactggcct cgacgcgcct gctcggcatg aacgagttcg cgctgcggct gccctcggcg
    19981 ctgtgctgca tcttcgcggt gctggtcacg atggccgtcg cgcaacgaac cctgagccga
    20041 agggcggcgc actacgcggg gatcgccgtc gccacgtccc tgtacgtcgt gatcgaggcg
    20101 cgttccctga ccgctgacgc gagcctgctc gccgcgacga ccgcgtcgtt ctgggcatgg
    20161 tcgcgattgc gggagcgtcc tcccgacccg ctgcgctggc aggtcctgct gtggtccagc
    20221 gtgggcttcg gcctgctcgc caaggtcgtc aacgtcgcgt tcatcggtgc cgccgcgtgt
    20281 gcgctcacgc tgatcgagac gagctggagc gggaggacgc gcgtggcgat cgcagtcgca
    20341 gtgctcgccg gagcgatcgc caccacgctg ccccgctgcg gctggatcgg tcccagcgtc
    20401 ctcggcgtgc ttgcgacgat cttcctgctg cattcgctgc tcgacgcatc ggcgcgcgcc
    20461 gaatggcgac gactcggcgc tgcctggggc attccgctcg ccgcgctcat gtttctcgcc
    20521 tggggcctgc cggccgcact cgctacgcac ggcgggttcg tcgcgcaggg gctcggccat
    20581 cacctgctcg cccgcaccgc cacgcccttc gaaggacatt ccggctggcc cggctattac
    20641 ctcgtggcca cgttcgtcgc gttctttccg tgggcttcgt tcctgccagc agcggtcacc
    20701 cgtgcttggt gtgaaagacg cgacccctgg cttgccttcc tgcttgcgtg gaccctcggg
    20761 ccgctggtgc tcgtcgaact cacgacctcg aagctgccgc actacctgct cgtcgccttc
    20821 cctgcgatat cgctgctcgt cgcgatgctg atcgacggtc gcagcgacgg gaaccgctcc
    20881 tggacgcgcc gcgagcgcgt cgtcgaggcg gctgtgttcg cggcgctttg cggttgcgtc
    20941 gccgtggcgt cgggctacgt cgccgcgagc ttcgggtccg cttcggtgcg cgcgccagcc
    21001 gccatcacgg ccgcagttgc gatcgcggtg gcggccgccg tcgtgcgcag ctggctgcac
    21061 gagccacccc gtcgactgct ggcccggctg gcgggcggag tcgtcatcct gtacctcgcc
    21121 gtgttcgcgg tgctgctgcc ggcgctcgag ccactgcggc ttgcgccgca actcggtgcc
    21181 gcggtcgccc aacgactcga gcccggggag cgactggtgc tctgcaggat ccgcgaggcg
    21241 agcgtcggtt tctatcttcc gcgcgtacct gatgccacgg gagacctcgg cacggtcacc
    21301 ctgcaattgc gcagctcccc gcgggatgcg ctcgtgctgg tcccggacga cgaacgcgga
    21361 ctgcccgctc gactccaggc cggcgagcag gaccgatggg agaggctcga ggccgtggac
    21421 ggcatcgtcc tgccggaact gtcgttgcga cggatgctgg tcctgcggcg ccacggcaac
    21481 gccggcgtgg agttgccgcg actggcggcg gaaactcagt cgcgcgtcag ccgcccgtaa
    21541 tccgtgaact tctcgatgac cttctgcagc tcctcgtcgt cgagcacgac gggttccatg
    21601 cccgcggcgc gcagcgcgag gtactgcgaa gccatgttct ccacctcgag cgcaaccgcc
    21661 ttggccgcag cgagcgacgg gccgacggcc acgacgccgt ggtttgccag cagcaccgcg
    21721 cgccgacctt cgagccccag caccgcgttg gctgcaagtt ccgcgctgcc gaaagtcgcg
    21781 tagggcatgc aacggacctc gccgccggcc aatgcgatca tgtagtggaa tgccgggatg
    21841 ccgacccccg cacaggacag tgcggtcgca ctcgggctgt gggtgtgcac gatcgcggcc
    21901 acgtccggac gctgcccgta gatcgccgcg tgcatgagcc actccgagga cggacgcagg
    21961 tcgccggcgc ccacggttcc gtcgtcgtgc agctcgacga gcaaggacgg ctcgagttcc
    22021 cggtacgcga cggcggacgg cgtgatgacg aatccgcccg gcgcccggca agagaggttg
    22081 cccgacttgt tgggcatcat gcccgcggcg tcgagctcgc acgcgacggt gacgatttcc
    22141 ttgcgcaggg tggcgtgtct agtggtcatc ccgttccccg gtgctcctgt ccgcgcgcga
    22201 cgccgcctca ggatacagcg actggagtcc tgcgcgcgac gccgcagcga tgccgcgctc
    22261 cgtgatgatg cccgtcacca gccgcgccgg cgtcacgtcg aatcccggat tcgcgacggc
    22321 actgcccggc cgcacgacct cgacctccag cacctgcccg ccgttggttc gtccccgtac
    22381 gtgcgtcacc tcgcgtgacg accgttcctc gatcggcacc tcgcgcacgc cgtcgtcgat
    22441 ctgccagtcg atcgtggagt acggcagcgc tgcatagaac ggcacgcggt tgtcgtgggc
    22501 tgcgagcgcc ttcaggtacg tgccgatctt gttggtcacg tcgccccttg ccgtgacacg
    22561 atcacttccg acgatgcaca ggtcgacgcg gccgcgctgc atcaggtggc cgccggcatt
    22621 gtcgacgatg acctcgtgcg gaatgccgtg cccggcgagt tcgaacgcgg tgagcgatcc
    22681 gccctggttg cgcggccgcg tttcgtccac gagcacgtgc acggcgatgc cggcctcgtg
    22741 cgccgcgtac acgggtgcga gcgctgtgcc ccaatcgaca caggcgagcc acccggcgtt
    22801 gcagtgggtc aggatgttga ccggccgacg tccggctccg gcgctcagct gctcgatgat
    22861 cgtggctccg tggcgcccga tggccgcgca ggcggcgaca tcgtcctcgg cgatgcggcc
    22921 ggcttccgcg tatgcgtgct gcacgcgctg cgcgatcggc aggggctgca gcgcctccgc
    22981 catccggccc agcgcccaac gcaggttgac cgccgtcgga cgcgccgcgc cgagcagcgc
    23041 cagggcggac tgcagcgatg catcggatgc gtcggatcgc atggcgagcg cgacgccatg
    23101 ggcggcggtc acgccgatga gtggggcacc tcgcacgatc atgtcgcgga tcgcgcgggc
    23161 tgcgtcctcg accgtgcgca ggtggcagac ctcgaattcg aacggcagca gcgtctggtc
    23221 gatcaccgtc acggtccagc cgtcgtcggc gacgaagatg ctgcgccagt gctcgccgtg
    23281 gacgcgcacc tcagtgtccc ccgagcacgc gccccgcgac cgcgtcgagt ttcgccagca
    23341 gcgcaggatc ccgcgcctcc ggccgcgtga ggatcgcgcc ttcgagggcg cgatcggagc
    23401 gcacggggca cgatacgtgc tcggccggga agtcccgcgc gagcctggcg aacaggcgct
    23461 gtgccttgcc acggttctcg tccatcactt tcagcagcgt cgacacctcc accgaatcgt
    23521 gcgtcgggtg ccagcagtcg tagtccgtca ccatgcagac gaccgcatag gtgatctccg
    23581 cctcgcgcgc gagcttggcc tccggcatcg atgtcatgtg gatcacgtcg tagccggcgt
    23641 cgcgatacga cagcgactcc gcgagcgtgg agaactgcgg accttccatg cacacggccg
    23701 tcccgccgcg atggaacgga atcccctccc cgtcggccgc gcgagccacg agctcctgca
    23761 gcgccggacc gaccggatgt gcgaatggca cgtgagcgac gcaacccgcg ccgaagaacg
    23821 aattcgcgcg ccgcaccgtc cggtcgacga agttgtccac cagcacgaag gtgccggggc
    23881 tgagttcctc gcggaaggat ccgcaggccg agaaggacac gaggtcggtg acgcccgcgc
    23941 gcttcatcgc gtccacgttg gcgcggtaat tgatgtcgga cggcgagtaa cggtgtcccc
    24001 ggccatgccg cggcaggaac acgacctcgg ttccgccgac gcgtccgaag cgcagcgcgt
    24061 cggacggctc accccacggc gtcgagacac gccgttcgct caggccctcg agcccgggaa
    24121 ggtcgtagat ccccgaaccg ccgatgattc cgagcactgc cttggtcatg gcccacctgc
    24181 ccgcgagtga cgacagtgta ccgtcccgcc ggcgggccgg cgtgcatcgt ctcgcgatcg
    24241 tgcggcagtc gacgcttacg ccagtgcggc gtgcaccccg acgcgcgtga cgcgggggat
    24301 gtcgaggtcg agcagcccct ggccgccgag cgacgcgacc cagcgccgca cctcggccgc
    24361 ctggtccgcc ggcatcgctg cgaccacctc cagcagccgc tgcgccatcc agagcatgta
    24421 cggcgcaccg tggcgcgtga agcgctcgcc tgcgtacgga tacgtcacct tgcccacgat
    24481 tcgcggcaga tgcccgcccc gaccgttctt catagggcgc accagctccg ccagggccgc
    24541 gagcatgggc accatctcgt ggaaggcgct gtccagcagc ggaaccagcg attcgggaat
    24601 cgcatcgtcg gcgaggtacg gcccgtcgga cggcgcgggc gtcgccgaca tcatccgctc
    24661 gctccatgcg tacaggcgcg gccgcgggcg cagcagccgc ctgctcgccg actcgtcgta
    24721 gcgcaggtgg ccgcagatcg gcccggccag cgagaagtcc gccagcgacg gcctggcccc
    24781 gaacacgtag ggcatcaagg cgaaatgtcg ctccaggtga tcgagctgct gcgccatcca
    24841 ccgctcgatg agcgcgacct ggtccggcgt cgcgccgagc tgggtgttca cgcgctgcat
    24901 gaaccacttg agcggcaggg gcagcagtcg ctgcacgaag gtcgggaagc ccggcgcaaa
    24961 agcggtcgcg agctcgtcgc gccacttcgg gtagttctcg ggaaagctcc agcgcgcgtg
    25021 cacgccggcc gacagccaga actcgtcggc ccacagctcg agcaggtacg ccgcgaaatg
    25081 ctggcgcggc gacgtcggca cgatcgggta gcccgggaat cgctcctcga ggcgctcgat
    25141 gatcacgctg ctgtcctgca gccattcgcc gtccggcgtg atcacgatcg gcaccacggc
    25201 atggccgcag cgccgcttga tttcgccgta gtattcgcgg gcggtcggcg tgcgctcgtc
    25261 gaacggcagc cgccggaatc gcaggtacgg gcgcaccttg gccgagtacc acgacagctg
    25321 catggaatag aggaagtacc ttccggtcga catcggtccg atcccgtgct cgtgagcggc
    25381 gcgaatccgt gccctggtgc ggtggccgcc gcgggccgcc atcgccggta cgaatgttgc
    25441 cataatcact ctgccccggc ggacggggcg aaggggggtt ccatggccac tgccgaccgc
    25501 gaccgcatcc cgcgcgacgc cgacgccgac gccgactata cgaagcaggc tgccgccagg
    25561 cgcgccgagt tcctgcgcga gaagacggga gccaccctgt cccacgtgca gggatacagc
    25621 ttcgaccccg gggtgctgcc cggcaacatc gagaatttca cgggcgtggc ccaggtgccg
    25681 atcggcatcg ccggaccatt gcgggtcaat ggcgagcacg cgcaggggga cttctacgtg
    25741 cccatggcga cgaccgaggg cacgctggtc gcgagctaca accgcggcat gcgggtgctc
    25801 gcggagagcg gcggcgtcac cactaccgtc gtcaagcgct cgatgcagcg cgcgccggtg
    25861 ttcatgttca cgacggcgcg cgaggcccgc gacttcggcg actggctgcg cacgaaccac
    25921 gagggcgtgc gcgatgctgc ccagtccacg acgcgcagcg ggcggctggt cgagatcgag
    25981 cagtacccgc tcgccaacat gctgtacctg cgcttcaact acacgacggg cgacgcggcc
    26041 gggcagaaca tgaccggcaa ggcgacgcgc gcggcgtgcg agtggatccg cgagagtcac
    26101 cctgcccacc cgaagtacat gctgtcgggc agcatcgaca ccgacaagaa gcactcggcg
    26161 atcaacacgc tgcgcacgcg cggacgacgc gtggtcgcgg aggccgtgat tcgcaaggac
    26221 gtcctgcagc gcctgatgcg ggtggacacg cagacgttgt tccgcgcacg gcaggtctcg
    26281 aatgcgggat cgatgctggc cggcagcgcc tacaacggac cgcacgcggc caacggcatc
    26341 acggccatgt tcatcgcctg cggccaggac gtggccaacg tcgccgagtc ccatgccggc
    26401 atcacctacg cgcagctgct cgacaatggt gactactact ggtcggtgac gctgccctcg
    26461 ctgatcgtcg cgtcctacgg cggcggcacc ggactgccga cgcagcgcga gtgcctcgag
    26521 gtgctcggct gctacggcac gggcaaggcg gacaagctcg ccgagatcgt ggccggtgtc
    26581 gtgctcgccg gcgacatctc gctgggttgc gcggtgctcg ccgacgagtg ggtcacggcg
    26641 cacgagcagc tcgggcgaaa ccggccctga gcgtcgccgt gcgcccgcgg cctgcctgac
    26701 ggaaatccgc gggtttttag gtatttttcg cgctctccgg ggcgcgactc acgcggcccg
    26761 gcgatatagg ctcgcgcgca ggaagccctt gtcttcccgc agcgccatcc gccatgagga
    26821 tcgaccgcta tgttcacgac gagcgcctga cgtggggcgc ggcgggcgtc ctgttgttcg
    26881 tgacggcggc gcacttcgcg ggcatcggtg ggttgctcgt agcgcgaccg ccgcaggtga
    26941 cgacggcgct gccgccgata ttccaggtga gcctggagaa gctccc
//
LOCUS       DAOMFD010000008        25927 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_290_length_25927_cov_30.338049, whole genome shotgun sequence.
ACCESSION   DAOMFD010000008 DAOMFD010000000
VERSION     DAOMFD010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 25927)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25927)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25927
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_290_length_25927_cov_30.338049"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(923..1528)
                     /locus_tag="PL152_00970"
     CDS             complement(923..1528)
                     /locus_tag="PL152_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47871.1"
                     /translation="MDSLPLAIMLGLLGIGVGLFVWQQVVHWALFASGLLDGLARKDV
                     SAIPKVVRLAKQLGAAWVITFGGAAAYLYLAAIPHGGFAGLLAGAAIVPILVLPRVIR
                     IARGERAAKSGRIRRLITKASRTLMGTMALMQVIWTPVMSALGMYLSYEDGALTVTEA
                     FRIVAIVSIGGAFATIPLYMLVSGMRPVSRDGRLKDSDSDS"
     gene            1771..2490
                     /locus_tag="PL152_00975"
     CDS             1771..2490
                     /locus_tag="PL152_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47872.1"
                     /translation="MSITEAMITSAKAGALIGAVGGTLPLVAGLCWGERKKAVLGLSA
                     CVLAGAIGGTYAVIPASLITFTAINRARQEKQAKQESMGLVFSYSRLIVWLGLLGMPV
                     LAVTSLVCIVVAAFGIARPDVTIGMKLNATFVGLLGVAFGWVTVVAARYWYALFTEYT
                     VTEEGVKIRYRASTDFAPWPSLRCAKYSKAFGQIELDFEGYARRVILSNVDLDPQRRR
                     VLEVLGLIERATGRPVLKSLL"
     gene            2630..2983
                     /locus_tag="PL152_00980"
     CDS             2630..2983
                     /locus_tag="PL152_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47873.1"
                     /translation="MTQTSPIVDLHTAMRLSSIALYLWPAIVTITIAAVRWRSMNHRF
                     GFLILGYLVCVGTGALFSHFGFVVYWLHFAPTVAEDNMVASLVNAAISVAVFGTLLSM
                     WPVLWLSRMLGAPTT"
     gene            complement(3525..5309)
                     /locus_tag="PL152_00985"
     CDS             complement(3525..5309)
                     /locus_tag="PL152_00985"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HQR47874.1"
                     /translation="MAKPADVFISYSREDKDRVLELATRLQAAGVSVWIDQGGIDGAT
                     LWGEEIVNALDRSKVLLLALTASAARSHNVAKEVVLVSERKGHILPVDLEPTSLPAGL
                     KYALAGIQHVEYFQGDPDENLRTILRSLERAGVSVGGRDVPLAAPKESAGVRAGAGAP
                     GAAEVAIESGAVAVLPFDNISADQETDYFSDGLTEELIARLSLISGIELVSRWASMQY
                     KGKAHDVRGIGNELGARYIVGGGVRRFQDSVRITVQLVDVATNRQIWGNTYKGKLDDI
                     FDIQEQVAQQIVDALKLKLTFSEKVSLTKRQTVNVQAYDFYLRGQDYLYRLTKKGTEY
                     AIQLFEKAIELDPRYGAAYAGCSCAYGQMYQWFSREEHYRDKAQEFSFKALMYDSNLP
                     EAYTAMGLSYFIWGKFEEAAASVRKAIELDPDDFVAHWTLGRIHFSTGALEAAIPHFE
                     RVIELRPRFYVAYSDLGQTLLGMGRTEEAADNARKVLDLMPNYLLQNPDDSRARMFYA
                     VTLCDVGQRDAAIREGAAAIEASPGDSVMLYNAACLYSRLGETRRAVDTLRQAVDAGV
                     RNYSWMKNDPDLDAIRGDPEFIALMAGN"
     gene            5492..5830
                     /locus_tag="PL152_00990"
     CDS             5492..5830
                     /locus_tag="PL152_00990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47875.1"
                     /translation="MRRFDSNNTGNEGRAMKKVSTMIAVVSAALLLAACAKKQEQAAT
                     AEQAADTAAAAADSATADAAAADAAAAASAAADAAAAAPATDGSAAPAGDAAAAPAAD
                     AAQPSSTTGN"
     gene            complement(5961..7346)
                     /locus_tag="PL152_00995"
     CDS             complement(5961..7346)
                     /locus_tag="PL152_00995"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQR47876.1"
                     /translation="MAHIAEAVATELSGGAELSRSLRSRHVSMIAIGGIIGAGLFVGS
                     STAINTVGPAAVVSYAVAGLIILLVMRMLSEMAIAIEGARTFPEFARVGLGHLAGFLS
                     GWLYWYFWVVVVAIEAIAGAKIISGWFPLLPVWLVGVGLMAVLTAVNLLSARSYGEFE
                     FWFSSIKVAAIIVFILVAGAFALGLTSGTGHSFANLTQHGGFAPHGWGVVLAGVTSTI
                     FSLCGAEIATIAAAESEEPAKTISRMTVSVSLRILIFYVLSILLIVAVVPWTQVQPGV
                     SPFATTLHFMGYGGADVIMNAVVLVAVLSCLNSGLYVTSRALFGLAKYGDAPQWLVQV
                     NRRKVPARAIIVASLFSYAALAAGRLSPDTVFSFLLNSSGVTMMLLYLLVSAAQLRLR
                     EQFEAQSPERLQIRMWAHPYGTWLAIVAMAAIMVFMGLTPDLASQLWASLLVAAGFAA
                     TYFLLRRRAPA"
     gene            7478..8953
                     /locus_tag="PL152_01000"
     CDS             7478..8953
                     /locus_tag="PL152_01000"
                     /inference="COORDINATES: protein motif:HMM:NF006112.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HQR47877.1"
                     /translation="MNRVSAVGLAALGAVALATIVITVRTVAFEPPAARDFGSVALAD
                     AAPVDVATAARHLSEAIRIRTVSHQDSSRNDWHEWDRFDQWLVATYPAANAAMRREVL
                     AGHTLVYTWPGANPALPPIVLMAHHDVVPVTPGTEGDWKHPPFDGVIADDAVWGRGSV
                     DDKGSLVGIFEAIEALATAGFKPQRSVIVLSGHDEEAGGSGAMTVAASWRSSGLAPQF
                     ALDEGLAVVSDLPLAGRPVALVGVAEKGYATLRITAPAKGGHSSAPPQETGVETLAKA
                     VLDVASHPFPMRFSGPTADMVRTLAPDAGLGVRVAVANTWLFEPLLIWKIAATPAGAA
                     TLHTTIAPTMLRGSPKENVLPQDATAWINYRIAPQDTAAQVLARATRATRRLDVQLAW
                     EGAAYDPSPVSSTRSDAYLVIGALASDGGRVPVAPGLVTATTDSRYLAGIARDVYRFQ
                     PIVASLREFEMVHGTDEHLTLDNLRRMTEFYSRLIATAAAR"
     gene            complement(8968..10683)
                     /locus_tag="PL152_01005"
     CDS             complement(8968..10683)
                     /locus_tag="PL152_01005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47878.1"
                     /translation="MPLVVAVTGHRDLVPAEVPAIRERVRALLEGLRNAYPYRAIMVM
                     SALAEGADRLVAEEALALDVLLVAVLPMSKDLYLEDFATDDSRAEFERLCAAAAETFE
                     LPLVSAGTARPGPESAVVREAQYAQCGVFLCAHCHILLALWDGKPSAQVGGTSQVVHF
                     HHRDVMPGYGSQAPASQLALTDDESDLVYHIVCSRNRPDGEPAAGLEPLTVSWFTTDD
                     AQPRTAELPARHRRVFERSTEFGREARERADDIERSSYPLLGNTDAAALAAGLIDIDR
                     VFCAADWLAIHYQRRFLLTLRASHVCVFLTGLAYISYTDFYSARLFLFLVLGLIVAGS
                     AINYVAARGAWHRKYLDYRTLAEGLRVQFYWAAAGVTTGSATKFAHDNFLQMQDAELG
                     WIRNVMRVAGTECDARPGAGRHGLEFTMREWIGDERSGQLGYYVRKAKERVIKRDTTH
                     RIARIGLWASAATLAVLMFVGAGVPMAVQAPLVYGMGCVLLAVGVRQSYAKGTAEAEL
                     IKQYEFMGRIFSNARRRIGEAGDDATKRAILKSLGDAALEEHAQWILMHRERSVGQED
                     IVRLS"
     gene            10706..>11034
                     /locus_tag="PL152_01010"
     CDS             10706..>11034
                     /locus_tag="PL152_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47879.1"
                     /translation="MPRTPVQGSKRLAAGLSCAMSIPAGPYWNAQRDAPGRFGVGRGL
                     APQQPGASPLVDRDLCRSPGGCNAGRDRGAAAGITIERGAPRAQAMNAAEQRLVDLLD
                     RWQASLER"
     assembly_gap    11035..11134
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11135..11320
                     /locus_tag="PL152_01015"
     CDS             <11135..11320
                     /locus_tag="PL152_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47880.1"
                     /translation="SAKVTEMVIADAVRLLSWGREWPQLAGLIARLADRPPEPEVWAI
                     LRAHRSSIQSKSRRAPG"
     gene            11421..11891
                     /locus_tag="PL152_01020"
     CDS             11421..11891
                     /locus_tag="PL152_01020"
                     /inference="COORDINATES: protein motif:HMM:NF013487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HQR47881.1"
                     /translation="MSSRSTFRRALPLAAALVLAGAAQADAPPSKGEQALTYRKAVYQ
                     VILWNVRPMTAMAQDKVPFNAQEFAMRAGRVAELAPMLSEAYPPESKGVANSKLKPEM
                     WANRADFDAKLKDLVDRSAALADVAKGGDAAKTKTAFFSLMDACKACHEKYKAD"
     gene            complement(11893..12612)
                     /locus_tag="PL152_01025"
     CDS             complement(11893..12612)
                     /locus_tag="PL152_01025"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HQR47882.1"
                     /translation="MQTAGQPKPAASRGAAARLVWDLPLRVTHWLIVITVAGAWATHY
                     AGTRWFAWHRTLGCATLVLVAFRIAWGFVGTRHARFATFLRGPRAVSAYVRERPRRPT
                     VGHNPLGALSVVAMLATLLVQAATGLFANDGIANAGPFYGWIAPATSNRISGLHEANS
                     TVLLALVVLHVLAIAWYGRVAGQPLTRAMLTGRKPATEVPPDASIADSRTVLALVIVV
                     ALALLLALAIRAAPDATIALY"
     gene            12792..13229
                     /locus_tag="PL152_01030"
     CDS             12792..13229
                     /locus_tag="PL152_01030"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQR47883.1"
                     /translation="MPAGLLRRCGALLYDLLLLAAVLMVVTALFLPFTGGEAITSAGN
                     PLLEWTYRAVLLGTIVLFYGAFWTGRGQTLGMAAWRLRVEREDGGRLTWGDTLRRLGT
                     AILSWLPAGLGYLWILVDPQRRAWHDRLSHTRVIVVPKRPRVR"
     gene            complement(13235..14302)
                     /gene="lptG"
                     /locus_tag="PL152_01035"
     CDS             complement(13235..14302)
                     /gene="lptG"
                     /locus_tag="PL152_01035"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HQR47884.1"
                     /translation="MPILARYILRTVFAYTLLVLAVLLVLGGLFLFINEQDDIGTGSY
                     TATEAMTYVLFNIPTFATQLLPVAALIGALLGLGNLARGSELVVMRASGITTFRFCLW
                     LAVAGILMAVVMAGVGEYVAPPLEKYGRQVKVFSKYNQFSFAGSRGAWVRDGDTIISV
                     EQQSAKSRFGGIQLFRFGPDRELLSAGRADAASVDDGRGWRLENYSETRFAGGGTEAR
                     KSPVEEFHSTLSPDFLGLAVVEPEAMGLRDLAAFIDHLRRNDLDSSGFEVALWSRIAR
                     MFALMLVLMLALPFSLGSMRSTGQGARMIVGIMIGAGFVLVSQTLENSGRLLGLSPPV
                     TGWLPTALLAVLTLTMLVRAR"
     gene            complement(14304..15395)
                     /gene="lptF"
                     /locus_tag="PL152_01040"
     CDS             complement(14304..15395)
                     /gene="lptF"
                     /locus_tag="PL152_01040"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR04407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HQR47885.1"
                     /translation="MLTRLDRYLLREVTLTFVAVTGVLLVILLSNQLARVLGQAVQDG
                     FPGRVVAGLIALTTLQQLTVLVPIGLYLGIVLALGRLYHESEMTAIAACGVGTASIYK
                     PVAILTLVVVAALAALSFRIVPNAGMQAQLIRIEALKAAQFGALEPGRFKTFAGGDIV
                     FYAERVLADGELKGVFVQRRVDDRVELAVAARAEQRGTGQSEQLFVLHDGRRYEGVPG
                     SLEWRVVEFKEHGIPVRLPEVRAKTDKLSYKPTRDLIGSSDNGDRSELAWRIAVPIMA
                     FVLMVLAVPLAKLSPRQGRFARVGFAVLAYFAYSNLVAAVRVWIDKGSPGGALGMWWV
                     HLVPLTLAAWLLWRDERPGPLLRWRKAGG"
     gene            15574..17058
                     /locus_tag="PL152_01045"
     CDS             15574..17058
                     /locus_tag="PL152_01045"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HQR47886.1"
                     /translation="MRFHVVSGSAARQRTDCAIVGLYESGTLGAAAQQIDAALGGRLT
                     RLVKRGDLRGKAGETTMLDTDRGPCGRVLVIGLGRKDGFDRRRYKKAMLAAAAAVVKS
                     GARDAVSYLSVEPVAATDAYYRARLAAEALGNALYRVPAIRSSRKPREPALRSFGVAV
                     PERDQKKDAERGLKHGRGIALGAALTRDLANLPANVCTPSYLGKRAHELAREYRTVRA
                     QVLGEPELRRLKMGSFLSVTHGTDEPARLIVLRYSGAAPRQAPVVLVGKGVTFDSGGI
                     SLKQPVGMDEMKFDMTGAASVFGAVKAAAEIGLPINIVGIVPACENMPSGRATKPGDV
                     VHSMSGQTIEVVNTDAEGRLILCDALTYARRFKPAVVVDIATLTGACVIALGAHLSAV
                     MSNDDELAAEIEEAGRRAEDRAWHMPMAEEYHDQLKSNFADFQNSCGREGGAITAACF
                     LAKFTEGMRWAHLDIAGTAWLGGAQKGSTGRPVPLLVDFLINRG"
     gene            17055..17522
                     /locus_tag="PL152_01050"
     CDS             17055..17522
                     /locus_tag="PL152_01050"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF016267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HQR47887.1"
                     /translation="MTVPDNAATKVDFYLVGATDARSRLATACRLAEKAFGQGLRVSV
                     RTASPSETAEVDELMWTFSDRSFVPHGVWPAEPEFAAATPVLIASGALPESHRDVLIN
                     LADDLPPDLGRYARVCEVVSGDEEARRRARIRWRGYRDAGLEPATHSLSERLA"
     gene            17519..17803
                     /locus_tag="PL152_01055"
     CDS             17519..17803
                     /locus_tag="PL152_01055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47888.1"
                     /translation="MNHTADTIPVLTDLIEDDALAGVPGTGLTATFLGEIEAHLSAAI
                     HDYADELVHNACRELEAMLLEQVSDRLRTQLPTLIAGIIEEHFRGPGSAG"
     gene            17816..20608
                     /locus_tag="PL152_01060"
     CDS             17816..20608
                     /locus_tag="PL152_01060"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003092872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HQR47889.1"
                     /translation="MDKSYSPRDIESRLYARWEASGWFAPSGAGAPYCIMIPPPNVTG
                     TLHMGHAFQHTLMDALTRMHRMDGDRALWQPGTDHAGIATQMVVERQLNAEGRSRQEL
                     GREEFLARVWQWKAQSGDTISRQMRRLGDSVDWSRDRFTMDEGLSAAVAEVFVRLHDK
                     GLIYRGKRLVNWDPVLHTALSDLEVVSEPESGSLWHLRYPLEDGTGQVVVATTRPETM
                     LGDVAVAVHPDDDRYRHLVGRRVRLPLTDRLIPVIADDYVDPAFGSGCVKITPAHDFN
                     DYEIGKRHGLPLINLFTASAVLNDAAPAAYRGLERFEARKRVVADLEAAGLLEKVEPH
                     SSPVPRGDRSGAVLEPWLTDQWYVRIAPLAEPAIRAVEDGRIRFVPDNWSKTYFQWMR
                     NIQDWCISRQLWWGHRIPAWYDDGGNVFVARSESEARAQARTRHGRDVPLVQDEDVLD
                     TWFSSALWPFSTLGWPEPTATLRSFYPTTVLVTGFDIIFFWVARMIMMGLEFTGEVPF
                     REVYITGLIRDEHGNKMSKSKGNIIDPLDLIDGIGLEELVAKRTTGLMQPQMRSSIEK
                     ATRKQFPQGIPAYGTDALRFTFASLATMGRDIRFDLGRIEGYRNFCNKLWNASRYVLM
                     SVGDDPAALAGDCEFSVADRWILGRLGRTVSAVREHFATYRFDLAAQSAYEFVWYEFC
                     DWYLELSKPVLQSADTAAALKRGTRRRLVEVLEATLRLLHPLMPFITEEIWQRVGALA
                     GRNGDTIMLEPYPRAADYPDDAQAARQVAVLQAVVLGVRQIRGELDVPHSRATPVFVR
                     TDHSGDAEAIDALAATIARVGNLESITVVASEADLPPCAIAISDGRTILAPFASLVDD
                     LSAERARLEKRRARAGQDRDKARAKLANESFVANAPPEVVDQERSRVAEFDRQLAQLA
                     EQLRRLESMTSRGETA"
     gene            20605..21564
                     /locus_tag="PL152_01065"
     CDS             20605..21564
                     /locus_tag="PL152_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR47890.1"
                     /translation="MSETDRAIVQLDADGVARVEAAVAALNRIILGKERQLRLCLACL
                     FARGHLLIEDIPGVGKTTLAHALAYTLGLSYQRIQFTSDLLPADITGVSVFDAESGQF
                     HFHRGPVFAQLVLADEVNRATPKAQSALLEAMEEHQVTSDGTTYPMSEPFFVIATQNP
                     MYQVGTFPLPESQLDRFLMRIELGYPEASLERELLLGADRRDLLAGLTPALTPQQLLR
                     IQGDVPRVHASGALLDYVQDVARYTRQASEFEAGLSPRAVIALLRAAQAWALMHARAG
                     VIPEDVQAVLPAVVGHRLAPRDASRFRDAGQVGRHVLESVPVP"
     gene            21571..22557
                     /locus_tag="PL152_01070"
     CDS             21571..22557
                     /locus_tag="PL152_01070"
                     /inference="COORDINATES: protein motif:HMM:NF013998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HQR47891.1"
                     /translation="MRDAIRRLRALADERMGAWIRRRQGADHDPVSLHRGRIYILPTR
                     LGGAYAVMVVAMVAGGLNYGNNLALGLAFVLASLGMVTMHHCHGTLSGLGLRLLATES
                     AFVGQPVRFRVLLENRSRSPRPQIEVAASDEAPDIADVPPVGTGTAGLALTAQVRGHV
                     HLRRFVVATRHPLGLFRAWAVVHPEYSAIAWPRPAERGRHPPGVETDTGGAQDRARGD
                     EDFAGLRPYQAGDALKRIAWKAYARGQGLHTKQYAGTDVVSHVFDWDSLPGLDAEARL
                     SQLCRWVIDAHESGEAFGIRLPGTAIEANIGTAHFQRCLDALALFERNEPHG"
     gene            22550..24505
                     /locus_tag="PL152_01075"
     CDS             22550..24505
                     /locus_tag="PL152_01075"
                     /inference="COORDINATES: protein motif:HMM:NF023418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3488 and transglutaminase-like
                     domain-containing protein"
                     /protein_id="HQR47892.1"
                     /translation="MAEPATSRLRGLDGVLVALAVSAAAHITQLPAWVSLLLVAAIAW
                     RWTADRRGAALPPRWLRNLVAVAAVVAVLATYRTINGVEAGTALLVLAASLKLLETRA
                     ARDLTILAFIAYFLLYAALLRDQRLPLLPLLLVGTLVTTAALMRVHAGSAGESWSTVA
                     RRSGILVLQALPFALALFLLFPRLPGPFWGIAAPNSARTGLSDEMTPGDVSDLSTSGE
                     PAFRVRFAGKLPPPALRYWRGPALHEFDGRTWRRPRGQAFPEQSVTTQGAPVDYQVTL
                     EPHDRNWILALDMPGGWPEREAYRAYDFTLVSPRPITDVASFQLRSYPRYRAGSVLPL
                     SLRHKDTQLPETGNPRSVALGRDLRARYGDPRQVAAAMLRLFRNQPFEYTLEPPRLGR
                     DSVDEFLFGTRKGFCEHYASAFTLVMRAAGVPARVVTGYQGGEWNALGGYLLVRQSDA
                     HAWSEIWLDDAGWVRVDPTAAVAPERIERGLVDAVGADEPAPGRLRQASALWYTATLA
                     WDTMNDFWNERVVRFNAARQFELLERLGIDEPDWRALGLALAASLAVFFVALSAQMAW
                     KFRGPVRDWPARLHDETARRLRRRGLEPGPVEGPIEFLDRASAACPDLSRELQQIRDL
                     YCGLRYGPRPAPEDLGRLKHLVNRLRP"
     gene            24556..24819
                     /locus_tag="PL152_01080"
     CDS             24556..24819
                     /locus_tag="PL152_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA-binding protein"
                     /protein_id="HQR47893.1"
                     /translation="MADLKKQFEAASKDAQKLPSRPSDDDMLRLYALYKQATAGDVSG
                     DRPGAFDFVNRAKYDAWARAKGTPTDKAMKSYVDLVARLKKLG"
     gene            24824..25687
                     /locus_tag="PL152_01085"
     CDS             24824..25687
                     /locus_tag="PL152_01085"
                     /inference="COORDINATES: protein motif:HMM:NF017932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent peptidase"
                     /protein_id="HQR47894.1"
                     /translation="MTPLAAVALAAAAVLAWLLAGPVIASRRRRAAMARPLDPQLVRL
                     IERNVPLRGRLPADLAARHDALVSAFLAEKEFVGCGGLEITDEVRATIAALACVLVIG
                     RRGHYDELHSVLVYPAAFWVEDEVEDEAGVVQKRRRDLSGEAWESSRIILSWEDVLES
                     AHFPGEGYNVALHEFAHYLDAEGLGLAPPDQSGTSGAARHRTRSLDAWSADLVQEFEA
                     FQSAVDREEQTFLDPYAAEDATEFFAVATEDFFERAAELRDAHPRLYELLQEFYALDP
                     ASWRASAAGLA"
     gene            complement(25722..>25927)
                     /locus_tag="PL152_01090"
     CDS             complement(25722..>25927)
                     /locus_tag="PL152_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47895.1"
                     /translation="FGWFSRKVSKGSRLRLVVSCPNSIYFEKNYNSGGRVEGESGKDA
                     RVAHVTVYHDRKHPSALEIPIVR"
BASE COUNT         4169 a         8981 c         8705 g         3972 t
ORIGIN      
        1 gcgtaacgta aatcttggag aagcgaccgc ccgcgcggcg gtccgcttcc gacccatttc
       61 ggacggtcag ccaactagtc acccgcgggt gcgcttggtg cgatccatca ttgcgtcgcg
      121 ttccgctggg ctaaagccga aaaagaatgc tacagacagt actagcgaac caatcgccaa
      181 aagtctccgc tcgtagggcg cgaaccattc tagcccgagg atcgaactgg tcatgatggg
      241 caaatagagc accaacaaga atcctaaagc ccacctcttg agttgtagac gcgagttccc
      301 ctcgggtgtc agcttgttgt tgggttccac gatcggagcc aacccaaact cgtggcgtag
      361 tcgcgcgaca gcggcgttca attcatcaga cgtgaccgca tctgcgcctg cgactcgata
      421 tgtcactgca ctcccaaaac cgtcgacatg tgcgcgaagg acgaatacca cagtattgcc
      481 cgttgtccac gcaccccgac cgacatcagt cacatcctct agacttatca ccacagtccg
      541 gcgaccgcgc ctgacagtca atttcctgcc acccaagtcg accgaatcgg cgatgaacca
      601 cgtcgtcgac cgcatgctca acgcaaagca gcctagaact aatgccacca ataggacgcc
      661 catgactagg ccaggtggtc gccgcgccaa ttggatggcg acaatgccga gggccactgc
      721 taggactatc agtgttacct tgaccttgta acgatgggtt ccgtcatcgg acaggcgtcg
      781 gctctcaatc atccgacctc cgcacttgtt cggacgcctg tcaggtgtcc ggttgtggct
      841 gagtccagtc gcataagcag ggccgattct tagcctcgca ccgcgctgaa ctcaagggcg
      901 gcttccgacc cgaagcaggc actcacgagt ccgaatccga gtccttcaat cggccgtcgc
      961 gcgataccgg ccgcattcca gacacaagca tgtaaagcgg tatggtcgcg aacgcgccgc
     1021 cgatgctcac aatggcaact atgcggaagg cttcggtcac ggtgagagcg ccgtcctcgt
     1081 agctgagata catgccaaga gccgacatca caggtgtcca tatcacctgc atcagcgcca
     1141 tcgtgcccat cagagtccgt gacgccttgg taatgagccg tctaattcgg ccactcttgg
     1201 ccgcgcgttc accccgcgca atccttatga cccgcggaag gacaaggatt ggcactatcg
     1261 ccgcacccgc gaggagtcct gcgaagccgc cgtgcggtat cgcagccaga tagagataag
     1321 ccgcagctcc gccgaacgta atcacccaag ctgcgccgag ctgcttcgcg agtctgacta
     1381 ccttcggaat cgccgaaaca tctttgcgcg ctaagccgtc cagtagccca cttgcgaaca
     1441 acgcccaatg cacaacctgt tgccagacga agaggccgac cccaattccg agcaagccca
     1501 acatgatggc aagcggcaag gagtccacac agttacccgt atgtcattgg tgaaatacga
     1561 tcctgccttg agtctaccgg cagccccgac cgtcggcttc tggccgaaag cggcccctgg
     1621 cggtagtcta atcggggtgg cggtgctgcg gcgcaacgaa tgtccgcaac cggccagaag
     1681 cggacaagat cgagatcgag gtatgcgggt acttggatcg aatctggttg ccaggatgct
     1741 gcggccggca tccgcaaaag ggagcgccga gtgtcgatca cagaagccat gattacgagc
     1801 gcgaaggccg gtgcgctaat cggagccgta ggtggcacgt tgccactagt cgctggactg
     1861 tgctggggcg aaagaaagaa ggctgtatta gggctgtcgg cctgtgtact cgcaggggcg
     1921 ataggtggca cgtatgcggt aatacccgcg tcgctaataa ccttcaccgc gatcaaccgt
     1981 gctcggcagg agaagcaggc gaagcaagag tcgatggggc ttgtattcag ctacagccgc
     2041 ttaatcgtct ggctcggtct cctaggcatg cccgtgctcg cggtcacgtc gttggtgtgc
     2101 atcgtggtcg cagctttcgg tatcgctcgg ccggatgtaa ctattggcat gaagttgaac
     2161 gccacgtttg ttggattact aggcgtcgcg ttcggctggg tgaccgtagt cgcagcaaga
     2221 tattggtatg cgttgttcac cgagtacacc gtgaccgaag agggcgtcaa gattaggtat
     2281 agggcgtcga cggactttgc gccctggcca tcacttcgtt gcgccaagta cagcaaggca
     2341 tttggacaaa ttgagcttga ttttgaaggc tacgctcgcc gggtgatctt gagcaatgtg
     2401 gacctagacc cgcagcggcg cagagttcta gaggtacttg ggctcattga gagagccacc
     2461 ggtcgcccgg tgctgaaatc actattgtga gccctgacaa gaggtctgct tcgggtcgag
     2521 aacggacatc ggaagtcggg caggataggc gcgtaagagg ccgtaggtag aatgcccgga
     2581 gccggccaga aacggacatt tgggttgtgt ctacggacgg gggattccag tgacgcagac
     2641 ctcaccgatc gtcgatctgc atacagcaat gcgcctgtcg agcattgcct tgtacctgtg
     2701 gccggcgatt gtgacgatca cgatcgccgc cgttcgttgg cgttccatga atcaccgctt
     2761 cggcttcctg atactcggtt acctcgtgtg cgtggggacc ggcgcacttt tcagtcactt
     2821 cggtttcgtt gtgtattggc tgcacttcgc accgactgta gccgaagaca acatggtggc
     2881 tagtttggtg aatgcagcca tcagcgtggc cgtgttcgga acgttgctca gcatgtggcc
     2941 tgttctatgg ctatcaagga tgctgggagc accgacgact taacggctag cggaccgtga
     3001 atggggctgc tttgggtcga aacccgccgt ccataatccg cgagaggtgg acgtcagctc
     3061 ctcggctaga gtgtcgctat gactaggcaa gggaagcgct cgtttattcc gcgagacaag
     3121 ccgaagtctt ggattgtgct gacgatcgcg tgcctcagcg ccctggcgtt tggcctcgtc
     3181 agtgtgcttc tgggcagcgc tttaggctct tcgacgggga aatttctggg ggcagcgttg
     3241 agcggctgct tcctgaccgc gctcgtcagc gcgttcgttt acatgcgcgg cttgtttacc
     3301 ggacgatacc gcgacctgca ggcccgacct tggcgggagc aagtgtggta agtcaggtcg
     3361 aattcagcat cttgaccgac gtccgcttcg ggtcggaagc ggacggtcac tgaagcggtc
     3421 cggtgcccgg cttcacacct actggcgcgc cggcctccct ccagccccgg cctcccgcac
     3481 tccagccgga ccgccgcggc gtcacccgcc gaagcctcgc gccgttaatt tcccgccatc
     3541 agcgcgatga attccgggtc gccgcggatc gcatccaggt ccgggtcatt cttcatccac
     3601 gagtaattcc gcacgccggc atcgaccgcc tgccgcagcg tgtcgacggc ccgccgcgtc
     3661 tcgccgagcc gcgagtagag gcacgcggcg ttgtagagca tcaccgagtc gccggggctc
     3721 gcctcgatcg cggccgcacc ctcgcggatc gccgcgtcgc gctggccgac gtcgcagagc
     3781 gtcacggcgt agaacatccg ggcccgcgag tcgtcgggat tctgcagcag gtagttcggc
     3841 attaggtcca gcaccttgcg ggcgttgtcc gcggcctcct ccgtgcgccc catcccgagc
     3901 agcgtctggc cgagatcgct gtacgccacg tagaacctcg gcctcagctc gatcacgcgc
     3961 tcgaagtgcg ggatggccgc ctccagcgcc cccgtcgaaa agtggatccg cccgagcgtc
     4021 cagtgcgcca cgaagtcgtc ggggtcgagc tcgatcgcct tgcgcacgga cgccgccgcc
     4081 tcctcgaact tgccccagat gaagtacgac agccccatcg ccgtataggc ctcgggcagg
     4141 ttgctgtcgt acatcagcgc cttgaagctg aactcctggg ccttgtcccg gtagtgttcc
     4201 tcgcgcgaga accactggta catctgcccg tacgcgcagg agcatccggc gtaggccgcg
     4261 ccgtagcgcg ggtcgagctc gatggccttc tcgaacagct ggatcgcgta ttcggtgccc
     4321 tttttcgtca gccggtacag gtagtcctgg ccgcgcaggt agaagtcgta ggcctgcacg
     4381 ttcaccgtct ggcgcttggt gagcgacact ttctcggaga aggtgagctt gagcttcagc
     4441 gcgtcgacga tctgctgcgc gacctgctcc tggatgtcga agatgtcgtc gagcttgccc
     4501 ttgtaggtgt tgccccagat ctggcgattg gtcgcgacgt cgacgagctg caccgtgatg
     4561 cgcaccgagt cctggaagcg ccgcacgccg ccaccgacga tgtaccgcgc gccgagctcg
     4621 ttgccgatgc cgcggacgtc gtgcgccttg cccttgtact gcatcgacgc ccagcgcgag
     4681 acgagctcga tgccgctgat cagcgacagc cgcgcgatca gctcctcggt gaggccgtcg
     4741 ctgaaatagt ccgtctcctg gtcggcgctg atgttgtcga atggcagcac cgccacggcg
     4801 cccgactcga tggccacctc ggccgcaccc ggggcaccgg cacctgcccg caccccggca
     4861 gactccttcg gtgccgccag cggtacgtcc cgaccgccga cgctgacccc cgctcgttcc
     4921 agcgagcgca ggatcgtcct caggttctcg tcggggtcgc cctggaagta ctcgacgtgc
     4981 tggatgccgg ccagcgcgta cttgagtccc gcgggcaggc tcgtgggctc gaggtcgacc
     5041 ggcaggatgt ggcccttgcg ctcgctcacg agcaccacct ccttcgccac gttgtgcgag
     5101 cgcgcagccg acgccgtcag cgccagcagc agcaccttcg agcggtccag cgcgttgacg
     5161 atctcctcgc cccacagcgt cgcgccgtcg atgccgccct ggtcgatcca gaccgacacg
     5221 ccggccgcct gcagcctggt cgcgagctcg aggacacggt ccttgtcctc gcgggaataa
     5281 ctgatgaaga cgtccgccgg cttggccatg cacgtgtcgc gtagcgtgga ccccgcggcg
     5341 ccttgccgcc ggggcgttct cccacctgag cggcgaaggc tagctcatcg tgccgggcct
     5401 gagaaggcaa accgcgacca acccgcaccc gccctgcgcg cgccgccccg ctcccggacg
     5461 cctgttacgc agcaccggct tcgattagga tgtgcgccgt ttcgactcca acaacaccgg
     5521 caacgaggga agggctatga agaaagtttc cacgatgatc gcggtcgtgt ccgcggcgct
     5581 cctgctggct gcctgcgcca agaagcagga acaggctgca accgcagagc aggcggccga
     5641 tacggctgct gccgctgcgg actccgccac cgcggacgct gccgcggccg acgcggccgc
     5701 tgcggcttcg gctgctgccg acgccgccgc cgctgcaccg gcgaccgatg gctccgcggc
     5761 tccggccgga gatgcggccg ctgcgccggc ggccgacgcc gcccagccga gcagcaccac
     5821 cggcaactga tcgtcagccg tgttgaagcg ggccgtcatc cggaatcgct ccgggtgacg
     5881 gccctttttc atggccgcga gttcggggcg ggtgcggctt catcgcaggg tcgaacccga
     5941 tcacgtcgac cagcgcggtt tcaggccggc gcccggcgcc gcagcaggaa gtaggtcgcc
     6001 gcgaatccgg cggccaccag caggctcgcc cacagctggc tcgcgaggtc gggcgtcagt
     6061 cccatgaaca ccatgatcgc cgccatcgcc acgatcgcga gccaggtgcc gtaggggtgc
     6121 gcccacatgc ggatctgcag ccgctccggt gactgcgcct cgaactgctc cctcagcctg
     6181 agttgtgccg cggacaccag caggtacagc agcatcatgg tcacgccgga ggaattcagc
     6241 aggaagctga acaccgtgtc cggcgacagg cgcccggccg cgagcgccgc gtagctgaac
     6301 aggctcgcca cgatgatcgc ccgggccggc accttgcgcc ggttgacctg cacgagccac
     6361 tgcggggcgt cgccgtactt ggcgagcccg aagagcgcgc gcgaagtgac gtacagcccg
     6421 gagttgaggc acgagagcac cgcgaccagc accacggcgt tcatgatcac gtcggcgcca
     6481 ccgtatccca tgaaatgcag cgtcgtcgcg aacggcgaca cgccgggctg gacctgtgtc
     6541 cagggcacga cggcgacgat gagcaggatc gacagcacgt agaagatcag gatccgcagc
     6601 gacacggaga ccgtcatgcg cgagatggtc ttcgccggct cctcggattc cgccgcggcg
     6661 atggtcgcga tctccgcgcc gcacagcgag aagatcgtcg aggtgacgcc cgcgagcacc
     6721 actccccagc cgtgcggcgc gaagccgccg tgctgcgtga ggttcgcgaa ggagtgaccc
     6781 gtgcccgagg tgagaccgag cgcaaaggcc ccggcgacca ggatgaagac gatgatcgcg
     6841 gcgaccttga tcgacgagaa ccagaattcg aattcgccgt acgagcgcgc cgacagcagg
     6901 ttgaccgcgg tgagcacggc catgagcccg acgccgacca gccagacggg cagcagcggg
     6961 aaccagccac tgatgatctt ggcgcccgcg atggcctcga tcgcgacgac gacgacccag
     7021 aaataccagt agagccaccc cgacaggaag cccgcgaggt gcccgagtcc gactcgcgcg
     7081 aattccggga acgtgcgcgc cccttcgatg gcgatcgcca tctccgagag catgcgcatc
     7141 acgagcagga tgatcagtcc cgcgacggcg taggacacga cggccgcggg accgacggtg
     7201 ttgatcgcgg tcgaggaccc cacgaacagt cccgcgccga tgatcccgcc gatcgcgatc
     7261 atggacacgt gccgcgagcg caggctgcgg ctgagttcgg cgccgccgct cagctccgtc
     7321 gcgacggctt ctgcgatgtg tgccacttcg ttcccccctg ccgccccttc gacggtgtgc
     7381 gggaggatac aggcccgccc gggactgtcc accttgacgc ggcgcgcagt tcagttcaca
     7441 aacgacgaac acaacctgca cttgcccggg accacccttg aacagagtct ctgcagtcgg
     7501 cctcgccgcc ctcggcgccg tggccctcgc aaccatcgtg atcaccgtgc ggacggtggc
     7561 cttcgagccg ccggccgcgc gcgacttcgg gtcggtcgcg ctggcggacg ccgcgccggt
     7621 cgacgtggcg accgccgcac ggcacctgtc ggaagcgatc cgcatccgca cggtcagcca
     7681 ccaggacagt tcacggaatg actggcacga gtgggatcgc ttcgaccagt ggctggtcgc
     7741 gacctacccg gccgcgaacg cggcgatgcg ccgcgaggtc ctcgccggcc acacgctggt
     7801 gtacacatgg cccggtgcga atcccgccct gccgccgatc gtgctgatgg cccaccacga
     7861 cgtcgtgccg gtcacaccgg gcaccgaggg tgactggaag cacccgccgt tcgacggcgt
     7921 cattgccgac gacgcggtgt ggggacgcgg atcggtcgac gacaaggggt cgctggtcgg
     7981 catcttcgag gcgatcgagg cgctcgcgac ggccggtttc aagccgcagc gcagcgtcat
     8041 cgtcctgagt ggccacgacg aggaggccgg cggcagcggc gcgatgaccg tcgccgcctc
     8101 gtggcgctcg agcggactgg cgccccagtt cgcgctcgac gagggtctcg cggtcgtgtc
     8161 cgacctgccg cttgcgggcc gcccggtcgc actcgtggga gtcgccgaga agggctatgc
     8221 gacgctgcgc attacggcac cggcaaaagg cgggcactcg tccgcaccgc cccaggagac
     8281 cggggtcgaa acgctggcga aggcggtgct cgacgtcgcg tcgcacccct tccccatgcg
     8341 cttcagcggc ccgacggccg acatggtccg cacgctcgcg cccgatgcag gactcggcgt
     8401 gcgcgtcgcc gtcgccaaca cgtggctgtt cgagccgctg ttgatctgga agatcgccgc
     8461 cacccccgcc ggcgccgcga cgctgcacac gacgatcgct ccgaccatgc tgcggggcag
     8521 cccgaaggag aacgtgctgc cccaggatgc cacggcctgg atcaactacc gcatcgcgcc
     8581 gcaggacacg gcagcgcagg tgctggcgcg cgccaccagg gcgacccgca ggctggacgt
     8641 gcagctcgcc tgggagggcg ccgcatacga cccgtcgccc gtctcgtcga ccaggtcgga
     8701 tgcgtatctc gtgatcggcg cgctggcctc cgacggcggg cgtgtcccgg tggcgccggg
     8761 gctcgtcacg gccacgacgg attcgcggta tctcgccggc atcgcgcgcg acgtctatcg
     8821 gttccagccg atcgtcgcgt cgctcaggga gttcgagatg gtgcacggca cggacgagca
     8881 cctgacgctc gacaacctgc gccgcatgac ggagttctac tcgaggctga tcgcgactgc
     8941 ggcggcaaga tagccgcggt cggtgcttca gctcaggcgg acgatgtcct cctggccgac
     9001 cgagcgctcg cggtgcatga ggatccactg cgcgtgctcc tcgagggctg cgtccccaag
     9061 cgacttcagg atggcgcgct tcgtggcatc gtcgccggcc tcgccgatcc ggcgtcgcgc
     9121 gttgctgaag atccgcccca tgaattcgta ctgctttatc agctcggcct ccgccgtccc
     9181 cttggcatac gactgccgca cgcccacggc cagcagcacg cagcccatgc cgtacaccag
     9241 cggtgcctgc acggccatgg gcaccccggc cccgacgaac atcagcaccg cgagcgtcgc
     9301 ggcgctcgcc cacaatccga tgcgggcgat gcggtgcgtg gtgtcgcgct tgatcacccg
     9361 ctccttcgcc ttgcgcacgt agtaaccgag ttgcccggat cgctcgtcgc cgatccattc
     9421 ccgcatcgtg aactcgagcc cgtggcggcc cgcgccgggc cgggcgtcgc actccgtccc
     9481 cgcgacccgc atcacgttgc ggatccagcc gagctccgcg tcctgcatct gcaggaaatt
     9541 gtcgtgcgcg aacttcgtcg cgctgccggt cgtcacgccc gcggccgccc agtagaactg
     9601 cacgcgcagg ccctcggcca gcgttcggta gtcgaggtac ttgcggtgcc aggcaccccg
     9661 ggccgcgacg tagttgatgg cgctgccggc gacgatcagc cccagcacca ggaacaggaa
     9721 caggcgggca gagtagaagt ccgtgtagga gatgtatgcg aggcccgtca ggaacacgca
     9781 gacgtggctc gcgcgcagcg tcagcaggaa tcgcctctgg tagtggatcg cgagccagtc
     9841 cgcggcgcag aacacccggt cgatgtcgat cagcccggca gccaatgccg ccgcgtcggt
     9901 gttgcccagc agcggatagc tggatcgctc gatgtcgtcg gcacgctcgc gggcctcgcg
     9961 gccgaattcc gtggaccgct cgaagacgcg acggtgccgc gcgggaagct ccgccgtgcg
    10021 cggctgcgcg tcgtcggtgg tgaaccacga gacggtgagc ggctcgagac ccgcggccgg
    10081 ctcgccatcc ggccggttgc gcgagcacac gatgtggtag acgaggtcgc tttcgtcatc
    10141 ggtcagcgcg agctggctcg ccggcgcctg ggagccgtag cccggcatga cgtcgcgatg
    10201 gtggaagtgc acgacctggc tcgtgccccc gacctgtgcc gatggcttgc cgtcccacaa
    10261 cgcgagcagg atgtggcagt gcgcgcacag gaacacgccg cactgcgcgt actgcgcttc
    10321 gcgcaccacg gccgattccg gccccggtcg cgcggtcccg gccgacacca gcggcagctc
    10381 gaaggtctcg gccgccgccg cgcacagccg ctcgaattcg gcgcgcgaat catccgtggc
    10441 gaagtcttcg aggtacaggt ccttcgacat cggcagcacc gcgaccagca gcacgtcgag
    10501 cgcgagcgct tcctcggcga cgagccggtc ggcgccctct gcgagcgccg acatcaccat
    10561 gatggcgcgg tacggatacg cgttgcgcag gccttcgagg agcgcacgca ccctctcgcg
    10621 gatcgccggt acctcggccg gcaccaggtc ccgatggccg gtgaccgcca cgacgagggg
    10681 cacggcgaat ccccggcgcg ccccgatgcc ccgcacaccc gtccagggat cgaagcgcct
    10741 cgcggccggg ttgtcgtgcg ccatgtccat cccggccgga ccatactgga acgcgcagcg
    10801 cgatgcaccc ggccggttcg gggtcggccg gggtcttgcg ccgcaacagc ccggtgcgtc
    10861 gcccctcgtg gaccgggacc tgtgtcgcag cccgggagga tgcaacgccg ggcgtgaccg
    10921 cggcgccgcc gctggcatta caatcgagcg tggcgcacct cgggctcaag cgatgaacgc
    10981 agcggaacag cggctggtcg acctgttgga tcgctggcag gcgagcctgg aacgnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcggcc aaggtcacgg agatggtgat
    11161 cgccgacgcg gtgcggctgc tgtcctgggg gcgcgagtgg ccccagctgg cggggctgat
    11221 cgcgcgcctg gccgaccggc cgcccgagcc ggaagtctgg gcgatcctgc gcgcgcatcg
    11281 ctccagcatc cagtcgaagt cgcggcgcgc gccaggctga ccggtcctcc gactcgaacc
    11341 gcggccgctg gcgatcgcgc ggtcgctgcg gtaaggtcgc gcgctccccg acccgtcacg
    11401 gccacaggag tgcccgaccc atgtcctcgc gttccacgtt ccgccgcgcc ctgcccctcg
    11461 ccgcagctct cgtgctcgcc ggtgccgccc aggccgacgc gcccccgtcc aaaggcgaac
    11521 aggcactcac ctatcgcaag gcggtctacc aggtgatctt gtggaatgtc cggccgatga
    11581 cggccatggc ccaggacaag gtcccgttca atgcccagga gttcgcgatg cgcgccggcc
    11641 gcgtggccga actcgcgccc atgctgtcgg aagcctatcc gccggaatcg aagggcgtgg
    11701 cgaattccaa gttgaagccg gagatgtggg ccaatcgggc ggacttcgac gccaagctca
    11761 aggatctcgt ggaccgcagc gccgcactcg ccgacgtcgc gaagggcggc gacgccgcga
    11821 agacgaagac cgccttcttc agcttgatgg acgcctgcaa ggcgtgccac gagaagtaca
    11881 aggccgactg attcaataca acgcgatcgt cgcgtcgggc gccgcccgaa tcgcgagcgc
    11941 cagcagcaag gcgagcgcca cgacgatgac gagcgcgagc acggtccgcg agtcggcgat
    12001 cgacgcgtcc ggcggcacct cggtcgcagg cttgcgcccc gtcagcatgg cacgcgtgag
    12061 cggctggccc gcgaccctgc cgtaccaggc gatcgcgagc acgtgcagga cgaccagcgc
    12121 gagcagcacc gtcgaattgg cctcgtgcag cccggagatg cggttgctcg tcgccggcgc
    12181 gatccacccg tagaacgggc ccgcattggc gatcccgtca ttggcgaaca gcccggtcgc
    12241 cgcctggacg agcagcgttg cgagcatggc gaccacgctg agcgccccga gcgggttgtg
    12301 cccgaccgtg ggtcgccgcg gtcgctcgcg cacatacgcg gacacggcgc gcgggccgcg
    12361 caggaatgtc gcaaatcgcg cgtgtcgcgt gccgacgaat ccccaggcga tgcgaaatgc
    12421 caccagcacc agcgtcgcgc agccgagcgt cctgtgccag gcgaaccatc gtgtccccgc
    12481 gtagtgggtg gcccaggcgc cggcgaccgt gatcacgatg agccagtggg tgacccgcag
    12541 cggcaggtcc cacaccagac gcgccgccgc gccacgcgat gcggcgggtt tcggttggcc
    12601 ggctgtctgc atcctccacc acctcgcggg tcgaccgggc gattctagca acgcgcacgg
    12661 gatgcgtgac gcggcgtact gcttgcacta atgtgccggc ctcgcatgcc gacccaccgt
    12721 cactcaatgc cgagcaccgt ccaggccgcg cacacgagcc ctcaccgctg cgacggggca
    12781 cgggaccgct gatgcccgcc ggcctgctgc gccgttgcgg cgcgctcctc tacgacttgc
    12841 tgctgctcgc ggccgtgctg atggtcgtca cggcgctgtt cctgccgttc acgggcggcg
    12901 aggcgatcac gagcgcaggc aatccgctgc tcgaatggac ctatcgcgcg gtcctgctcg
    12961 gaacgatcgt gctgttctac ggggcattct ggacgggtcg tggccagaca ctgggcatgg
    13021 ccgcctggcg gctgcgcgtg gagcgcgagg acggcggacg gcttacgtgg ggcgacaccc
    13081 tgcgccggct cgggaccgca atcctctcgt ggctgccggc ggggcttggc tacctctgga
    13141 tcctcgtcga cccgcagcgg cgcgcgtggc acgaccgcct gtcccacaca cgcgtgatcg
    13201 tcgtgccgaa gcggcctcgc gtgcgctgaa cgggtcagcg agcccgcacc agcatcgtca
    13261 gcgtcagcac ggccagcagc gcggtcggca accagcccgt caccggcggc gagaggccga
    13321 gcagccggcc gctgttctcg agcgtctggc tcaccagcac gaaacccgcg ccgatcatga
    13381 tgccgacgat catgcgggcg ccctgccccg tcgagcgcat cgatccgagc gagaacggca
    13441 gcgcgagcat gagcaccagc atcaacgcga acatgcgcgc gatccgcgac cagagtgcga
    13501 cctcgaagcc tgacgaatcg aggtcgttgc gtcgcaggtg gtcgatgaag gcggcgaggt
    13561 ctcgcaggcc catggcctcg ggctcgacga ccgcgagccc gaggaagtcc ggcgacagcg
    13621 tcgaatgaaa ttcctcgacc ggcgacttgc gcgcctcggt cccgccaccg gcgaaacgcg
    13681 tctcgctgta gttctcgagc cgccagccgc ggccgtcgtc gacactggcc gcgtccgcgc
    13741 gaccggccga gagcaactcg cgatccggcc cgaagcggaa cagctggatg ccgccgaagc
    13801 gcgacttggc ggactgctgc tccacgctga tgatcgtgtc gccatcgcgg acccacgccc
    13861 cgcgactgcc ggcgaagctg aactggttgt acttgctgaa gaccttgacc tggcggccgt
    13921 acttctcgag cggcggcgcg acgtactccc cgacgccggc catgacgacg gccatcagga
    13981 tgccggcgac cgcaagccac aggcagaagc ggaaggtggt gatgcccgac gcgcgcatca
    14041 cgacgagctc gctgccgcgc gcgaggttgc cgagcccgag cagcgcgccg atcaacgcgg
    14101 cgaccggcag cagctgggtc gcgaaggtcg ggatgttgaa cagcacgtag gtcatcgcct
    14161 cggtcgcggt gtagctgccg gtgccgatgt cgtcctgctc gttgatgaac aggaacagtc
    14221 cgccgagcac gagcagcacc gccagcacga gcagggtgta cgcgaacacc gtgcgcagga
    14281 tgtagcgggc caggatcggc atctcagcct cctgccttgc gccagcgcag cagcggaccg
    14341 ggccgctcgt cgcgccacag cagccacgcc gcaagcgtca gcggcaccag gtgcacccac
    14401 cacatgccga gcgcgcctcc cgggctgccc ttgtcgatcc acacgcgcac ggcggcgacg
    14461 aggttggagt aggcgaaata cgcgagcacg gcgaagccca cccgcgcgaa ccggccttgc
    14521 cggggactca gcttcgcaag cggcaccgcc agcaccatca gcacgaacgc catgatcggc
    14581 acggcaatgc gccaggcgag ttcggagcgg tcgccgttgt cactcgagcc gatcaggtcg
    14641 cgtgtcggct tgtacgacag cttgtcggtc ttcgccctga cttcgggcag gcgcaccggt
    14701 atgccgtgct ccttgaactc gacgacccgc cactccagcg acccgggcac gccctcgtag
    14761 cgccgcccgt cgtgcagcac gaacagctgc tcggactgcc cggtgccacg ctgctcggcg
    14821 cgcgccgcaa cggccagttc cactcgatcg tccacgcgtc gctgcacgaa cacgcccttg
    14881 agttcgccgt cggcgagcac tcgctccgca tagaagacga tgtcgccccc ggcgaaggtc
    14941 ttgaagcggc ccggttccag cgcgccgaac tgcgccgcct tgagcgcctc gatgcggatc
    15001 agctgggcct gcatgccggc gttcgggacg atccggaagg acagcgcggc gagcgcggcg
    15061 acgacgacca gggtcaggat cgcgacgggc ttgtagatgc tggccgtgcc gacgccacag
    15121 gcggcgatgg ccgtcatctc gctctcgtgg tacagccggc cgagggcaag cacgatcccg
    15181 aggtacagcc cgatgggcac cagcaccgtg agttgctgca gcgtcgtgag cgcgatgagg
    15241 cccgccacca cccggcccgg gaacccgtcc tggacggcct gtccgagcac gcgcgcgagc
    15301 tggttcgaca gcaggatcac cagcagcacc ccggtgaccg ccacgaacgt cagtgtcacc
    15361 tcgcgcagca ggtagcggtc aaggcgggtg agcacaggtc gcggctccgt gcgggaaacg
    15421 gtagactccg gcgaccgccg gcggcgggcc gggattcacc cggcgagcct gcgacgggcg
    15481 gcgcgcaatg caagtgtagc ggtcgcgacc gcccggccac cagccggctt cgcgccgccc
    15541 gggattcggt caccacttcg acaaggaaca agaatgcgat ttcacgtcgt gagcggctcg
    15601 gccgcacgcc agcgcacgga ttgcgcgatc gtgggactgt acgaatccgg cacgctcggc
    15661 gcggctgctc agcagatcga cgccgcgctg gggggacgtc tcacgcgact cgtgaagcgc
    15721 ggcgacctgc gcggcaaggc gggcgagacg acgatgctcg acaccgaccg cggaccctgc
    15781 gggcgtgtgc tggtgatcgg cctggggcgc aaggacggct tcgaccgccg gcgctacaag
    15841 aaggccatgc tcgcggcagc ggcagcggtc gtgaagtccg gggcgcgcga cgccgtgagc
    15901 tacctgtcgg tggaaccggt cgccgcgacg gacgcgtact accgcgcgcg actggcggcc
    15961 gaggcgctcg gcaacgcgct ctatcgcgtg cccgcgatcc gcagctcgcg caagccgcgg
    16021 gagccggcgc tccgcagctt cggtgtcgcc gtgccggagc gcgaccagaa gaaggacgcc
    16081 gagcgcgggc tcaagcacgg ccggggcatc gcgctcggcg ccgccctcac ccgggacctc
    16141 gccaacctgc ccgccaacgt gtgcacgccg agctacctcg gcaagcgcgc ccacgagctc
    16201 gctcgcgagt accgcaccgt ccgggcgcag gtgctcggcg aacccgaact gcgacggctc
    16261 aagatgggat cgttcctctc cgtcacccac ggcacggacg agcccgctcg cctgatcgtg
    16321 ctgcgctatt cgggcgccgc gccgcggcag gcgccggtgg tcctggtcgg caagggcgtg
    16381 accttcgaca gcggcggcat ctcgctcaag cagccggtcg gcatggacga gatgaagttc
    16441 gacatgaccg gtgcggcgag cgtcttcggt gccgtgaagg cggcggccga gatcgggctg
    16501 ccgatcaaca tcgtgggcat cgtgccggcg tgcgagaaca tgccgagcgg ccgcgccacc
    16561 aagcccggcg acgtcgtgca cagcatgtcc gggcagacca tcgaggtcgt caacaccgac
    16621 gccgaggggc ggctgatcct gtgcgacgcc ctgacctacg cgcgccgctt caagcccgcg
    16681 gtggtcgtcg acatcgccac gctgaccggc gcctgcgtca tcgcgctcgg cgcgcacctg
    16741 agcgccgtga tgagcaacga cgacgagctc gcggccgaga tcgaggaggc cgggcgtcgc
    16801 gcggaggacc gcgcgtggca catgccgatg gccgaggaat accacgacca gctcaagagc
    16861 aacttcgcgg acttccagaa ttcctgcggc cgcgaggggg gcgcgatcac ggccgcgtgc
    16921 ttcctggcca agttcaccga gggaatgcgc tgggcgcacc tcgacatcgc gggcaccgcg
    16981 tggctcggcg gggcccagaa aggcagcacc gggcggcccg tgccgctgct ggtggacttc
    17041 ctgatcaatc gcggatgacc gtgccggaca acgcagcgac caaagtggac ttctacctgg
    17101 tcggcgcgac cgatgcgcgc tcgcgcctcg ccaccgcctg ccgcctggcg gaaaaggcct
    17161 tcggccaggg cctgcgcgtc tcggtgcgca cggcgagccc gtcggagacc gccgaggtgg
    17221 acgagctcat gtggacgttc agcgatcgca gcttcgtgcc gcacggggtg tggcctgcgg
    17281 agccggagtt cgcggccgcg accccggtgc tgatcgcgag cggcgccctg cccgagtcgc
    17341 accgcgacgt gctgatcaac ctggccgacg acctgccgcc cgatctcggc cgctacgcgc
    17401 gggtgtgcga ggtggtgtcg ggcgacgagg aagcgaggcg ccgggcgcgc atccgctggc
    17461 gcggctaccg ggatgccggg ctcgaaccgg ccacgcacag tctgagcgag aggttggcat
    17521 gaaccacacc gcggacacga ttcccgtcct gaccgacctc atcgaggacg acgccctggc
    17581 gggcgtgcct ggcacgggcc tgacggcgac gttcctgggt gagatcgagg cgcacctgtc
    17641 cgcggcaatc cacgactacg ccgacgagct cgtgcacaat gcctgccgcg agttggaggc
    17701 catgctcctc gagcaggtct ccgaccggct gcgcacccag ctgcccacgc tgatcgccgg
    17761 catcatcgaa gagcatttcc gcggaccggg cagcgccggc tgacgattca ggacgatgga
    17821 caagagttac tcgccgcgcg acatcgagtc gcgcctgtac gcacgctggg aggcatcggg
    17881 ctggttcgcg ccgagcgggg ccggcgcgcc ctactgcatc atgatcccgc ccccgaacgt
    17941 gaccggaacg ctgcacatgg gccacgcgtt ccagcacacg ctgatggacg ccctcacgcg
    18001 catgcaccgc atggacggcg accgcgccct gtggcagccg ggcacggacc acgccggcat
    18061 cgccacgcag atggtcgtgg agcgtcagct caatgccgag ggccgctcgc gccaggaact
    18121 cggtcgcgag gagttcctcg cccgcgtgtg gcagtggaag gcccagtccg gcgacacgat
    18181 cagccgccag atgcgccgcc tcggcgactc ggtcgactgg tcacgcgacc gcttcacgat
    18241 ggacgagggc ttgtcggccg ccgtggccga ggtcttcgtc cggctccacg acaagggcct
    18301 catctatcgc gggaagcgcc tggtcaactg ggacccggtg ctgcacacgg cgctttcgga
    18361 cctggaagtc gtgtcggagc cggagtccgg cagcctctgg cacctgcgct acccgctcga
    18421 agacggaact ggccaggtcg tcgtggcgac gacgcgcccg gagaccatgc tcggcgacgt
    18481 cgcggtggcc gtgcatccag acgatgatcg gtaccgacat ctggtagggc gacgcgtccg
    18541 gctgccgctc accgaccggc tcatcccggt gatcgccgac gattacgtcg atcctgcgtt
    18601 cggcagcggt tgcgtgaaga tcaccccggc gcacgacttc aacgattacg agatcggcaa
    18661 gcggcacggg ctgccgctca tcaatctgtt cacggcgagc gcggtgctga atgacgcggc
    18721 cccggccgca taccgcggac tggagcgctt cgaggcgcgc aagcgcgtcg tcgcggacct
    18781 cgaagccgca ggattgctgg agaaggtcga acctcacagc tcgcccgtgc cgcgcggcga
    18841 ccgctcgggc gccgtgctcg agccgtggct caccgaccag tggtacgtgc gcatcgcgcc
    18901 gctcgccgag ccagcgatcc gcgcggtcga ggacggccgc atccgcttcg tgccggacaa
    18961 ctggtcgaag acgtacttcc agtggatgcg caacatccag gactggtgca tcagccggca
    19021 gctgtggtgg ggtcaccgaa tccccgcctg gtacgacgac gggggcaacg tgttcgtcgc
    19081 gcgcagcgag tcggaagcgc gggcgcaggc gcgtaccagg cacgggcgcg acgtgccgct
    19141 cgtgcaggac gaggacgtac tcgacacctg gttcagctcg gcgctgtggc cgttctcgac
    19201 gctggggtgg ccggaaccaa cggcgacact ccgctcgttc tacccgacga ccgtgctcgt
    19261 cacgggcttc gacatcatct tcttctgggt ggcccggatg atcatgatgg gcctggagtt
    19321 caccggggag gtgccgttcc gcgaggtgta catcaccggg ctgatccgcg acgagcacgg
    19381 caacaagatg tcgaagtcca agggcaacat catcgacccg ctggacctga tcgacggcat
    19441 cggcctcgag gaactggtcg cgaagcggac caccgggctg atgcagccgc agatgcgcag
    19501 ctcgatcgag aaggcgacgc gcaagcagtt cccgcagggc attcccgcgt acgggacgga
    19561 cgcgctgcgc ttcaccttcg cctcgctcgc gaccatgggg cgcgacatcc gcttcgacct
    19621 cgggcgcatc gagggctacc gcaacttctg caacaagctc tggaacgcgt cgcgttacgt
    19681 gctcatgagc gtgggcgacg accctgcggc gctcgcgggt gactgcgagt tcagcgtcgc
    19741 cgaccgctgg atcctgggac ggctcggccg cacggtgtcc gccgtgcgcg agcacttcgc
    19801 cacctatcgc ttcgacctcg cggcgcagtc cgcgtacgaa ttcgtgtggt acgagttctg
    19861 cgactggtac ctggaacttt cgaagccggt cctgcagtct gcggacacgg ccgccgcgct
    19921 gaagcgcggc acgcggcgcc ggctggtcga ggtgctcgag gcgaccctgc ggctgctgca
    19981 ccccctcatg cccttcatca ccgaggagat ctggcagcgc gtcggggccc tcgccggccg
    20041 caacggcgac acgatcatgc tcgagcccta tccgcgcgca gccgattacc cggacgatgc
    20101 ccaggctgcg cgacaggtcg ccgtgctgca ggcggtcgtg ctcggcgtgc ggcagatccg
    20161 cggcgagctc gacgtgccgc actcgcgcgc cacgcccgta ttcgtccgca ccgaccattc
    20221 gggggatgcc gaggccatcg acgcgctcgc ggccaccatc gccagggtcg gcaacctcga
    20281 gtcgatcacc gtcgtggcgt ccgaagcgga cctgcccccc tgcgccatcg cgatcagcga
    20341 cggccgcacg atcctcgccc cgttcgcgag cctcgtcgac gacctctccg cggaacgcgc
    20401 ccggctcgag aagcggcgcg cccgggcggg ccaggaccgt gacaaggcgc gtgccaagct
    20461 cgccaacgag agcttcgtcg ccaatgcgcc gccggaggtg gtcgaccagg agcgctcgcg
    20521 cgtcgcggag ttcgaccgcc agctcgcgca gctcgccgag cagttgcgga gactcgagtc
    20581 catgacgtca cgcggcgaaa ccgcatgagc gagaccgatc gcgccatcgt gcagctcgac
    20641 gccgacggcg tcgcccgcgt ggaggccgcc gtcgccgcgc tcaaccgcat catcctcggc
    20701 aaggagcgac agctgcggct gtgcctggcg tgcctgttcg cccggggcca cctcctgatc
    20761 gaggacatcc ccggggtcgg caagaccacg ctggcgcacg cgctcgccta cacgctggga
    20821 ctcagctacc agcgcatcca gttcaccagc gacctgctgc cggccgacat cacgggggtc
    20881 tcggtcttcg atgccgagtc gggccagttc cacttccacc gcggcccggt gttcgcgcag
    20941 ctcgtgctcg ccgacgaggt caatcgcgcc acgcccaagg cacagagcgc gctgctcgag
    21001 gcgatggagg agcaccaggt cacgtccgac ggcacgacct acccgatgag cgagccgttc
    21061 ttcgtgatcg cgacccagaa cccgatgtac caggtgggca cgtttccact gccggagtcg
    21121 cagctcgacc ggttcctgat gcgaatcgag ctcggctatc cggaagcctc gctcgagcgc
    21181 gagctgctgc tcggcgcgga ccggcgcgac ctgctggccg gcctcacgcc ggcgctgacg
    21241 ccgcagcagc tgctgcgcat ccagggggac gtccctcgcg tgcatgcgtc gggagcgctg
    21301 ctcgactacg tgcaggacgt cgcgcgctac acccggcagg cgagtgaatt cgaggcggga
    21361 ctttcgccgc gcgccgtcat cgcgctgctg cgtgccgcgc aggcgtgggc gctgatgcac
    21421 gcccgcgccg gagtgatccc ggaggacgtc caggcggtgc tgcccgccgt cgtcggccac
    21481 cgcctcgcgc cgcgcgacgc gtcgcgcttc cgcgacgccg ggcaggtcgg acggcacgtg
    21541 ctcgagtccg tccccgttcc ctgaggcggg atgcgcgacg cgatccgacg cctgcgcgcg
    21601 ctcgccgacg agcgcatggg cgcctggatc cggcgccggc aaggtgcgga ccatgatcct
    21661 gtctcgctgc accgcggacg catctacatc ctgccgacgc ggctcggcgg tgcgtacgcc
    21721 gtcatggtcg tggcgatggt cgcgggcggc ctcaattacg gcaacaacct cgcgctcggc
    21781 ctcgcattcg tgctcgcgag cctcggcatg gtcaccatgc atcattgcca cgggacgctg
    21841 tcggggctcg gactgcgatt gctcgccacg gagtcggcct tcgtcggaca gcccgttcgg
    21901 tttcgcgtcc tgctcgagaa tcggtcgcgg tcgccccgac cgcagatcga agtggcagcc
    21961 tccgacgagg caccggacat cgccgacgtg ccgcccgtcg gaaccggaac tgccggcctc
    22021 gcgttgactg cgcaagtgcg cgggcacgtg cacctccggc ggttcgtggt cgcgaccagg
    22081 catccgctgg ggctgttccg ggcctgggcc gtcgtgcacc cggagtactc ggccatcgcg
    22141 tggccacgcc cggccgagcg cgggcggcat cccccgggcg tcgagaccga taccggcggc
    22201 gcgcaggacc gtgcgcgagg cgacgaggat ttcgcgggac tgcgtcccta ccaggcggga
    22261 gacgcgctga agcgcatcgc gtggaaggcc tatgcgcgcg gccagggact gcacacgaag
    22321 cagtacgccg gcaccgacgt ggtgtcgcac gtgttcgact gggacagcct gccggggctc
    22381 gacgccgagg cgcggctgtc gcagttgtgc cgctgggtga tcgacgccca cgagagcggc
    22441 gaggcgttcg gcatccggct gcccggcacc gcgatcgagg ccaacatcgg cacggcccac
    22501 ttccagcgct gcctcgacgc gctcgcgctc ttcgagcgga atgaaccgca tggctgagcc
    22561 ggccacctcg aggttgcgcg gactggacgg cgtgctggtc gccctcgccg tgtcggcagc
    22621 cgcacacatc acgcagctgc cggcctgggt gtcgttgctg ctcgtggccg ccatcgcgtg
    22681 gcgatggacg gcggaccgcc gcggtgccgc cctccccccg cgctggttgc gcaatctcgt
    22741 cgccgtcgcg gcggtggtgg ccgtgctggc gacgtatcgc accatcaacg gcgtcgaggc
    22801 cggtaccgcc ctgctggtgc tcgcagcgtc cttgaagctc ctcgagaccc gcgcggcgcg
    22861 tgacttgacg atcctcgcgt tcatcgccta cttcctgttg tacgccgccc tgctgcgcga
    22921 ccagcggctg ccgctgctcc cgctcctgct cgtgggaacg ctggtgacca cggcggccct
    22981 gatgcgcgtg cacgccggat cggccgggga gtcgtggagc acggtcgcgc ggcggtccgg
    23041 catcctcgtg ctgcaggcgc tcccgttcgc gcttgcgctg ttcctgctgt tcccgcgcct
    23101 gcccggaccg ttctggggca tcgcggcccc gaactcggcc cgcaccggat tgtcggacga
    23161 aatgacgccg ggcgacgtct cggacctgag cacgtcgggc gagccggcct tccgcgtccg
    23221 cttcgcgggc aagcttccgc cgccggccct gcgctactgg cgcgggccgg ctttgcacga
    23281 attcgacggc cgtacctggc ggcgcccgcg cggccaggcc ttcccggagc agtccgtcac
    23341 gacgcaggga gcgcccgtcg actaccaggt cacgctcgag ccgcatgacc gcaactggat
    23401 cctcgcgctc gacatgcccg gcggctggcc ggagcgggaa gcgtaccgcg cctacgactt
    23461 cacgctggtc tccccgcgcc cgatcaccga cgtcgcgtcg ttccagctgc gctcataccc
    23521 gcgctatcgc gccggctccg tcctgccatt gtcgctgcgt cacaaggaca cgcagctgcc
    23581 ggagaccggc aacccgcgct cggtcgcgct gggacgcgat ctgcgcgcac gctacggcga
    23641 tccccggcag gtcgccgccg cgatgctgcg gctgttccgc aaccagccgt tcgagtacac
    23701 gctggagccg ccccggctcg gccgcgactc ggtcgacgag ttcctgttcg gcacccgcaa
    23761 gggattctgc gagcactacg cgtcggcgtt cacgctggtg atgcgtgcgg ccggcgtgcc
    23821 ggcgcgcgtc gtcaccggat accagggtgg cgagtggaat gcgctcggcg gctacctgct
    23881 ggtgcgccag tcggacgcgc acgcgtggtc ggagatctgg ctggacgacg ccggctgggt
    23941 ccgcgtcgat cccaccgcgg cggtcgcgcc ggaacgcatc gagcgaggac tggtcgacgc
    24001 cgtcggggcc gacgaacccg cacccgggag gctccggcag gccagcgcgc tctggtacac
    24061 ggcgacactg gcctgggaca cgatgaacga cttctggaac gagcgcgtcg tgcgcttcaa
    24121 cgccgcgcgc cagttcgagc tgctcgagcg cctcggcatc gacgagcccg actggcgggc
    24181 gctcggtctc gcgctcgcgg ccagcctcgc ggtgttcttc gtggcactgt cggcgcagat
    24241 ggcctggaag ttccgcggac cggttcgcga ctggcccgca cgactgcacg acgagaccgc
    24301 gcggcggctc cggcgaaggg ggctcgagcc cggcccggtc gagggcccga tcgagttcct
    24361 cgaccgcgcg tcggccgcct gtccggacct gtcccgcgag ctgcagcaga tccgtgatct
    24421 ctactgcggc ttgcgttacg ggccacgacc cgcccccgag gacctgggcc ggctcaagca
    24481 cctcgtcaac cggctccgcc cctgagcgcc ggtcgggtat gcttggtccc tcgtgcgtct
    24541 tcgagttgca ggaccatggc agacctcaag aaacagttcg aagcggccag caaggacgcg
    24601 cagaagctcc cgtcgcgtcc gtccgacgac gacatgctgc gcctgtacgc gctctacaag
    24661 caggcgaccg cgggcgacgt gagcggcgac cggcccgggg cgttcgattt cgtgaatcgc
    24721 gcgaagtacg acgcgtgggc gcgcgccaag ggcacgccga cggacaaggc gatgaagtcc
    24781 tacgtggacc tcgtcgcgcg cctgaagaaa ctgggctgac gcgatgaccc cgcttgccgc
    24841 ggtcgcgctc gccgcggccg cggtcctcgc ctggctgctc gccgggccgg tgatcgcatc
    24901 gcgccgccgg cgcgcggcga tggcaaggcc actggacccg cagctcgtgc ggctcataga
    24961 gcgcaacgtt ccgttgcgcg gccggctgcc cgcggacctc gccgcgcgcc acgatgcact
    25021 ggtcagcgcc ttccttgccg agaaagagtt cgtcggctgc ggtggcctcg agatcacgga
    25081 cgaggttcgc gccacgatcg cggcgctggc ctgcgtgctg gtgatcggac gccgggggca
    25141 ctacgacgaa ctgcactccg tgctggtgta cccggcggcg ttctgggtcg aggacgaagt
    25201 cgaggacgaa gcgggcgtgg tgcagaagcg tcgccgcgac ctctcgggcg aggcgtggga
    25261 atcgagccgc atcatcctgt cgtgggagga cgtgctcgag tcggcgcact ttcccggcga
    25321 gggctacaac gtcgcgctgc acgagttcgc gcattacctg gatgccgagg ggttggggct
    25381 cgcgccgcct gaccagagcg gcacctccgg cgcggcgcgc caccggacgc gatcgctgga
    25441 cgcgtggtca gcagatctcg tgcaggaatt cgaggcgttc cagtccgcgg tggaccgtga
    25501 agagcagacg tttctcgatc cctacgcggc cgaggacgcg acggaattct tcgcggtcgc
    25561 aacggaagac ttcttcgagc gggcagccga attgcgcgac gctcatccgc gcctgtacga
    25621 gctgctgcag gagttctatg cgctcgatcc cgcgtcgtgg cgcgcgagtg cggccgggct
    25681 ggcctgagca ggctccggcc ggcgcgccga gctcgaccgg ctcaacgcac gatcgggatc
    25741 tccagcgcgc tcgggtgctt gcgatcgtgg tacacggtca cgtgcgcgac ccgcgcgtcc
    25801 ttgccggact cgccctcgac gcgcccgccc gagttgtagt tcttctcgaa gtagatgctg
    25861 ttcgggcaac tcacgacgag ccgcaggcgg ctgcccttcg acaccttgcg cgagaaccac
    25921 ccgaacc
//
LOCUS       DAOMFD010000009        25620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_298_length_25620_cov_26.603677, whole genome shotgun sequence.
ACCESSION   DAOMFD010000009 DAOMFD010000000
VERSION     DAOMFD010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 25620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25620
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_298_length_25620_cov_26.603677"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="PL152_01095"
     CDS             complement(<1..446)
                     /locus_tag="PL152_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47896.1"
                     /translation="MQPSAHDKLVRQFRQILLWPLQLLPIRHGMQIQRHWELLEQPGP
                     DNPWHEVADEFTGDPARFQERHYTEFTTFLPHVQRFLYGEGREQRARVGGPADPGASP
                     MRVFRRDDVAAVRLVPEAGSEPVTLSLVHVDLYFFFDIDIVLLNVE"
     gene            553..1299
                     /locus_tag="PL152_01100"
     CDS             553..1299
                     /locus_tag="PL152_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47897.1"
                     /translation="MSESEPGLSVATWVLALGGAGFAAGFFGPMALNPGANQGPMAGI
                     FITGPGGALLGLVLGVASRLLGAGLARQRQALISAAAVLSLGTLYACLPEPETEGVLI
                     EGSVQTCQPPAELEEAAIQDWQRRIEAAPWGQPRDGWREGVDRMLRDADGVVLTVDVQ
                     REDRILRHRKPWNAGRKTVAGWTQANEARRYYAAFAGRDCASYSGPIQILYVRYGQGS
                     GVWPPDDAPNFLGVARVEPAPAALLALTQH"
     assembly_gap    1360..1459
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1700..1996)
                     /locus_tag="PL152_01105"
     CDS             complement(1700..1996)
                     /locus_tag="PL152_01105"
                     /inference="COORDINATES: protein motif:HMM:NF018717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HQR47898.1"
                     /translation="MSVYVVAQITIHDRAGYSRYEEGFLGVFQLFKGELLAVSENPTV
                     VEGQWPCTRTVLIRFPSQEEAARWYQSPEYQAIAQHRFRASTGNAVIVEGLTSS"
     gene            complement(2082..2378)
                     /locus_tag="PL152_01110"
     CDS             complement(2082..2378)
                     /locus_tag="PL152_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47899.1"
                     /translation="MRLHELTAASALVIASALAPPAYASTAPDQTTTCQLLAAARHIG
                     GDAQHTFVTECVRSSTIALNGMAGRQAACEELADTDHLVGAARRSFIADCKTFR"
     gene            2604..2999
                     /locus_tag="PL152_01115"
     CDS             2604..2999
                     /locus_tag="PL152_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HQR47900.1"
                     /translation="MAVRIVRLGSQRLAGEGLRIGTVRRPPRGVPKSDFAKRDFYDVW
                     LPNLAPSEELLKAAHAAIGADDERAWKAFVRRYRAEMKRPDSRALLQTLAALSHTADF
                     SVGCYCADEARCHRSVLRELLAEHGARLA"
     gene            3596..>4850
                     /locus_tag="PL152_01120"
     CDS             3596..>4850
                     /locus_tag="PL152_01120"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQR47901.1"
                     /translation="MLLAPGDATRWFVVEQGGRVRVFDNQASVSTTRSFVDITARVQS
                     GGETGLLGMAFHPNFPTNPRVYLSYTNGSSGLVSRVSEFRSTDGGQTLDPTTEVILLT
                     VTQPESNHNGGNIAFGPDGYLYIGFGDGGGGGDQHPPIGNGQNLTTTLGKLLRIDIGG
                     STGSVPYRIPADNPYVGNALCSTGSGAQNCPEIYAYGFRNPWRWSFDRVSGELWVGDV
                     GQNLIEEVDRVVAGGNYGWRCFEGTSTYNSNCGPNAGSSLPPVAQYDHSVGSSITGGY
                     VYRGSAIPALAGRYVFGDFGSGRIWNIARDTAPTLTVTGTPAFASGRSISSFAQDSAG
                     ELYIVDYGGALYRLDNAAAGGGSVPAKLSDSGCVQPGDPTKPAAGLVPYAPNAAFWSD
                     GASKDRYVALPNGTSATVGTDGDMDL"
     assembly_gap    4851..4950
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4951..5733
                     /locus_tag="PL152_01125"
     CDS             <4951..5733
                     /locus_tag="PL152_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47902.1"
                     /translation="ALPNGTSATVGTDGDMDLPVGTVLMKEFRLGSRRVETRFLMRHT
                     DGEWAGYTYEWNAQGTDATRVVGGKTAVIGTQTWVFPSEAQCLSCHTSAAGRTLGLEL
                     AQLNGLLLYPQTGRTANQVVTWNAIGLLNPPVGGAPDQWASLPDPYGSAGTLAERARA
                     YLHSNCSQCHRPGGGTPSDMDLRYATPLASTNACDRPPQAGDLGIANARIIAPGSATR
                     SVLVSRVGRRDAQAMPPLASTVVDAAGVSLLTNWVNSLASCN"
     gene            5750..5956
                     /locus_tag="PL152_01130"
     CDS             5750..5956
                     /locus_tag="PL152_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47903.1"
                     /translation="MLKFLLWLLLFVVCWPLAIAALVLWPIVWLLSLPFRLVGIAVEG
                     MFAFLRALVMLPARVLGSRPALRE"
     gene            complement(5983..>7663)
                     /locus_tag="PL152_01135"
     CDS             complement(5983..>7663)
                     /locus_tag="PL152_01135"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR47904.1"
                     /translation="ATVNPTRFERWRSMTLAPFEHRVFALFWWASLVSSFGSLIQTVG
                     ASWLMTTIAPSVDQVALVQTAGSLPFFFLSLVAGALADTRDRRAIMLISQILAVFASV
                     ALAVVVLAGRITPSLLLTLTFLIGCGGALFAPAWQASIGDQVPRAQIAPAVMANAVGF
                     NLARSLGPAIGGVIVAAFSAAAAFIINAVSYLGIIATLLWWRPARAHSELPPEPLGTA
                     IAAGVRYISLSPHLLSIMLRCVLNTIPIAAVPALMPIVARDLLGGGATTYGLLLGGFG
                     VGAMLGALASAPLRSQFTHDGLLRLLSALACVAMVGAGLSRWMALTLLMHVLAGTAWT
                     LAFANFNIAVQLSSPRWVTARMLAVYQTVAFASIALGSWWWGRFAADAGLRESLVVAG
                     VSALVSLVAARWLPIAVEQLGSLDPRVRIERNPPSADIHPSSGPIVVSIEYRVPAEHA
                     VEFVAVINEVGRIRRRDGARAWSICQDIDAPDLWVERFESPTWMDHLRWRTRPTESDQ
                     AVRARLGRLIVSEHSRVRRFVVRPPGADPLGTHPHAGHDATHAARDPHGRW"
     assembly_gap    7664..7763
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7932..9386
                     /locus_tag="PL152_01140"
     CDS             7932..9386
                     /locus_tag="PL152_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR47905.1"
                     /translation="MKTLSGLDGMFLHLETPETPMHVGSLIVLQLPKGYRGDFLADVK
                     RLYARRAALAPVLKRTLRELPLQLANPAWVQADTLDLDYHFRRVVLPKPGTQAQLEAC
                     VGHLHSVPLDRAHPLWSVTIIEGLPGRKVGYYTNIHHAVIDGQSGVELAKVVFDQTPR
                     PRRMPRMLADAAHGEHPWPGAAVAGALRHDAAQLAKLVQHLPEITRTIAAMRRAASRV
                     GTEEPARTERRFAPRTTFNAAIRAARGFAGVSIPLGEVHRIAAAHRATVNDVVLAICA
                     GALRRYLGRHGGVPLDSLIAAVPISLREAGNVEYTTLATMARVSLATDVADPVRRLHA
                     IRDAASAAKAATGRARSILPTDFPTLGLPWLLHGLATLYGAAHLADVVPPLFNLVISN
                     VRGPPMPLYIAGARVVRYWPLSIVAQGLGINITVESYAGSLEFGVTTAASAVRNPRAL
                     GAALLAAHRQLLARTIRGRPGRGKTRTRAVRGLG"
     gene            9483..9653
                     /locus_tag="PL152_01145"
     CDS             9483..9653
                     /locus_tag="PL152_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47906.1"
                     /translation="MGNTLRGLRHKPQLPGLAASLRTLNAWMRALVIQTAAVQAVWSA
                     ALQRNLDRGRRI"
     gene            complement(9982..10386)
                     /locus_tag="PL152_01150"
     CDS             complement(9982..10386)
                     /locus_tag="PL152_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47907.1"
                     /translation="MDQVSTADLLNLLLAQRSSIDLQFQFWLTITFAVLAAAFVAGDR
                     LRWGLRWLAALLYALASAHLILRWMYDGTVGQHWIEELNRRGVDIAIPWPAVYLRMTL
                     MGIGTVSSLGFLLTAAKRHPRLGASSESDRIP"
     gene            10511..10909
                     /locus_tag="PL152_01155"
     CDS             10511..10909
                     /locus_tag="PL152_01155"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR47908.1"
                     /translation="MLAVLAARPKNRYLAQQRYRIHRPRMTDESKLAPLRKGLLEFLV
                     LKIVGAERMYVPDILRRLAGTEFSTQEGTLYPLLSKLRREDLVQYEWKESDAGPPRKY
                     YRLTAAGRMQLKALDEYWQQINRTVASIGH"
     gene            10913..11491
                     /locus_tag="PL152_01160"
     CDS             10913..11491
                     /locus_tag="PL152_01160"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HQR47909.1"
                     /translation="MQRISITVRLNRSTLQFDEAAFARLEQYLAESAGLLEGDPDPQE
                     ILGDLEQAVADQCTRRLPPERSVVTLAELEPALAEIGTVQVPGTASTAQDRPAPAASR
                     PLQQISEGAVISGVCQGLARYFGLNVTALRVFAVLLLLLSGGGVIVVYLALMLLMPYA
                     PLRSDGPSLSWLPEKCRTLTEFLRAKFHLATS"
     gene            complement(11563..11943)
                     /locus_tag="PL152_01165"
     CDS             complement(11563..11943)
                     /locus_tag="PL152_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQR47910.1"
                     /translation="MAKAKKSPKKSAPKAAAKAAPKAVKTVLSKSALVKHLAGVSGAA
                     AKDVRAVIHALEHTIHASISKKGPRTFVLPGILKITAVAVPAKKARKGINPFTGQETV
                     FKAKPATVKVKVRPMKKLKDAATA"
     gene            complement(12044..12511)
                     /locus_tag="PL152_01170"
     CDS             complement(12044..12511)
                     /locus_tag="PL152_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1203 domain-containing protein"
                     /protein_id="HQR47911.1"
                     /translation="MTFTIRGLPIARFSHLLALDSAALAAVGVSVVTADSADGYPCRI
                     SLEDAAPGEELLLLSYQHQPGPTPYAAAGPIFIRRKASATRIARDTIPDQQRRRLLSV
                     RAYDDRNWIVAADVTPGNELESLIHRYFGRDDVAYLHVHNARPGCFACRVDRA"
     gene            complement(12700..12822)
                     /locus_tag="PL152_01175"
     CDS             complement(12700..12822)
                     /locus_tag="PL152_01175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47912.1"
                     /translation="MFARQASDRFLDVALAGLTIAVTVVMAAATHAIVAAQSFI"
     gene            13012..14109
                     /locus_tag="PL152_01180"
     CDS             13012..14109
                     /locus_tag="PL152_01180"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HQR47913.1"
                     /translation="MKITIDNMRRYAVARSLFRPTTLGRAIRRLGFVQADPIRAPARA
                     QDLTLRHRVVDYRAGDLERRYARLGLEEDFFVNYGFLRREHSALMHPRTPRRPWSAER
                     RRQAEAVLEFVRQRRSVHPREVDAAFAHGKTRNWFGGSSNASTQLLDEMHYRGLLRVV
                     RREGGTRVYAVRSHTAPSASDDTAVATRMDELVDLIAMKYAPLPARSLGYYLSLLCTG
                     VPQWRSRRADALARARQRLGRARVGAEDWFWPADENPGSRRWRPDGAVRLLTPFDPIV
                     HDRHRFERLWDWRYRFEAYTPPSKRSLGYYALPLLWGHRVLGWGNLSVVDGALRSEFG
                     YVGGVAPGDPGFQSELRAELERMQVFLGING"
     gene            14175..14651
                     /locus_tag="PL152_01185"
     CDS             14175..14651
                     /locus_tag="PL152_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR47914.1"
                     /translation="MTARAALLATLVLAALLAAVPSWAGYTYVPAPGLPASDLVQPPE
                     LAALLADPSARKPLVLQVGAHVLYVQAHIPGSEYVGPGEDDAGLAALRSRVARLPRDA
                     SIVIYCGCCPWDHCRNIGGAYAQLKRMGFTRVKVLYIASNFVSDWVDAGYAVATGD"
     gene            14752..15879
                     /locus_tag="PL152_01190"
     CDS             14752..15879
                     /locus_tag="PL152_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR47915.1"
                     /translation="MNAFGKKPDGLRLERMQASPLWHGGTFRNMHPLLPRLLKPNVAA
                     PTVSAFLFEGARRVPTAPLPAVDPTGAWRRKPGSGLRATWLGHSTVLLEIDGLRVLTD
                     PVWSLRASPSQWVGPKRFQPVPVSLRSLPHLDAVVISHDHYDHLDYRAIRELARREVP
                     FVTSLGVGAHLEDWGVPPKRIHELDWWESYALPRADLSVTAAPSQHFSGRGLRSRNST
                     LWSSMVIRSQRHSVFFSGDTGLTTEYAEIRRRLGPFDLVMLEVGAYHPAWGHIHLGPD
                     NALEALDLLGGGAFLPVHWGTFSLAMHAWDDPAETLFAEAPRRGVQLVMPRLGEAVEP
                     AHVDRVKPWWRSVEQAQRRAPKGLQEPRDTPPPKSMPWPID"
     gene            15893..16312
                     /locus_tag="PL152_01195"
     CDS             15893..16312
                     /locus_tag="PL152_01195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HQR47916.1"
                     /translation="MQTVLTIRTQGPGLYEFTADVARFVHASGVREGLVTLFVQHTSC
                     SLCVQENADPDVRRDLDEFFRRLVPSSDDPSMRWIAHTTEGPDDMPAHIKAALNAVSL
                     AVPVTDGRLALGTWQGIYLFEHRDRGQARRVFAHLTA"
     gene            16544..16903
                     /locus_tag="PL152_01200"
     CDS             16544..16903
                     /locus_tag="PL152_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47917.1"
                     /translation="MKLDDGKQWATDAPLRSGMAAIRKAFDADHPAIHAGEETDAQYE
                     VLAGKIEAQVNDIVRNCHLPPAADANLHFVIADLLQGVNLMRGTNAARSRHDGAALVH
                     GALIAYGQYFEDPDWRH"
     gene            16916..17659
                     /locus_tag="PL152_01205"
     CDS             16916..17659
                     /locus_tag="PL152_01205"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HQR47918.1"
                     /translation="MATVLVPLPSRDFDPTETGVPWKSLRALGHRIVFATPDGQPGTA
                     DDRMLTGRGLGVLARFLIADANGRQAHAEMAASEEFRRPITHTTARVADYDGLLLPGG
                     HAPGMRTYLESKPLQALIVAAFDRGLPVGAICHGVVLAARSRRNDGRSVLCGRKTTAL
                     TKQMELTAWALTCTWLGRYYRTYPMTVEDEVTAALANPTDFLRGPLSTARDRPEDLAA
                     GFTVRDGNYLSARWPGDAHRFASEFARML"
     gene            complement(17705..18586)
                     /locus_tag="PL152_01210"
     CDS             complement(17705..18586)
                     /locus_tag="PL152_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HQR47919.1"
                     /translation="MYRSLLFVPGDSEKKITKAMTVGADVVILDLEDAVADSRKAAAR
                     GIVREALRADRARRGCSLFVRVNPLGSPLCHEDLAAVMAGRPDGLVLPKAASADDTVR
                     LSNRLDILETENGIGAGATRIMPVATEVPEALFTLGTYRDAGPRLAALTWGAEDLAAA
                     VGAIDNKEPDGRWSAPFELARSLCLFAASAAGVPAIDTLHADFRDSTGLAESSRRARR
                     DGFAGKLAIHPDQVAVINSAFTPTAEEVARARRIVELFEANPGAGALSLDGRMLDMPH
                     LVQARKTIALAERLTSS"
     gene            complement(18589..19050)
                     /locus_tag="PL152_01215"
     CDS             complement(18589..19050)
                     /locus_tag="PL152_01215"
                     /inference="COORDINATES: protein motif:HMM:NF024844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQR47920.1"
                     /translation="MAGLYFEEFHVGQVFRHAIRRTITEADNVFFTALTHNPAAIHLD
                     EEYCREHSEFGTRVVNSVLTLGLMVGISVGDTTLGTTVGNLGWDEVRFPRPLFHGDTV
                     HVESEVVELRDSKSRPQNGIIVFQHRAFNQRNELVGVCRRTALMLRKPVGA"
     gene            complement(19056..19397)
                     /locus_tag="PL152_01220"
     CDS             complement(19056..19397)
                     /locus_tag="PL152_01220"
                     /inference="COORDINATES: protein motif:HMM:NF019401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HQR47921.1"
                     /translation="MSDYQHVMELVSDAVDAFGVLIMVLGATIATVRFALAQRAEFDG
                     AYRGYRQDLGRGILLGLEFLVAGDIIRTVAVAPTLQNVAVLGLIVVIRTFLSMSLQLE
                     VDGRLPWQREI"
     gene            complement(19416..20087)
                     /locus_tag="PL152_01225"
     CDS             complement(19416..20087)
                     /locus_tag="PL152_01225"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HQR47922.1"
                     /translation="MLKLHGFPVSNYVNMVEMALLEKGVPYEYVLVMPDQSPEWLAKS
                     PRGKVPALETPQGYLNEASVILEYLEDTGQGRPLLPADPYARGLVRALMKEIELYIEL
                     PARSCFVEAFFGGKMPEPIKAMARENLLAGFATLKRHGKFTPYVAGDTFTLADIVFMF
                     SADLAAGVGAKLWGIDLLAEMPAAKALLQRLGELPHARAIAARREAAMPAFIAATRAK
                     FAKAG"
     gene            complement(20113..20571)
                     /locus_tag="PL152_01230"
     CDS             complement(20113..20571)
                     /locus_tag="PL152_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HQR47923.1"
                     /translation="MEMPRPTAGHAQLERLAGLWKGEETMHPSQWDPNGGTATARLDY
                     RMALGGFALVTDYEQQRDGHVTYQGHGVMTYDPATTTYNLHWFDSMGAPPEVFSGRFD
                     GDELVVAHGGPGMHARMTYDLSTAGTLKMRMDMSRDGTRWMTLFDGEYRR"
     gene            complement(20656..21018)
                     /locus_tag="PL152_01235"
     CDS             complement(20656..21018)
                     /locus_tag="PL152_01235"
                     /inference="COORDINATES: protein motif:HMM:NF018893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HQR47924.1"
                     /translation="MPGYVDGFVVPVPKRKVAAYRRMSRLAGKVWREHGALDYVECMA
                     DDVKPGKWTSFPQSVKLRKGEVVWFSWILYRSRRDRDRINAKVMRDPRLASMMDPKNM
                     PFDGKRMFWGGFKIMVRA"
     gene            complement(21078..21521)
                     /locus_tag="PL152_01240"
     CDS             complement(21078..21521)
                     /locus_tag="PL152_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR47925.1"
                     /translation="MSLLIRPLGASERGEWEPLARAYKDFYQTVLPDTAYDGTWRRLT
                     AGDGIRGLGARLDGRLVGIAHYLYHTGIWAPRACYLEDLFVAPAARGQGVARALIEAI
                     AQQARADGAGRLYWLTHETNATARMLYDRVARYSGFVEYEYPMDA"
     gene            complement(21518..>21774)
                     /locus_tag="PL152_01245"
     CDS             complement(21518..>21774)
                     /locus_tag="PL152_01245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47926.1"
                     /translation="WRFNGQQHPNAIHMCVTGPQTRPGLVEEFAADLDAAVAYAGNPS
                     RAKPASSGIYGGGLDMDLSHPAVAQAFFTHAMDAFTDCPL"
     assembly_gap    21775..21874
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<21875..23091)
                     /locus_tag="PL152_01250"
     CDS             complement(<21875..23091)
                     /locus_tag="PL152_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR47927.1"
                     /translation="MDIEKAMRALYPYAEEFGVTRRMPQHGMDTASVLGQLRSMAQRE
                     DRTWESGKCSGTMYCGDHDHYAALNEAFASFSHVNALQRDMCPSMNRLESEIVAMTLD
                     MLHGEAVAGHDAKRKACGVLGFGGTESILNAMLAYREWARAERRITRPRMIWPDTAHP
                     AFRKAAHLFGLEVDVAPTDPVTTQVDVDFVRRSITPETAVIVASAGNYPYGTIDPISE
                     LSEVAVAAGVGLHVDGCLGGWILPWGQELGVPDIPVFDFRLPGVTSISADTHKYGYGL
                     KGTSVLAWQDKSLRRHQYYMLTDWKGGAYASPGLAGSRSGGLIAATWASMMMLGRDGY
                     LERARKIFDAAFAMQAAVRSHPELRIMGRPTFCFSFTSDAFDIYHVNDFMKTRGWRFN
                     GQQHPNAIHMCVTG"
     gene            complement(23121..23921)
                     /locus_tag="PL152_01255"
     CDS             complement(23121..23921)
                     /locus_tag="PL152_01255"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQR47928.1"
                     /translation="MNDREISECLEVGLAAVLEAGPVALQYFRKDPVAHDKSTFGHYD
                     PVTEADRRVEAVIRGRLAQRYPAHRIVGEEHGESGSGPVAWYIDPIDGTASFVSGMPT
                     WGILLGLVVDGRAVAGIMHQPYTGETYLASPGIGAVHRAHDRETRLRTRATATMADAT
                     VYSTHPRMFVDPALRAAYDRLAAQCRLQRWGGDCYSLALLAHGCIDLVVEGILMPYDF
                     FPMVPIIERAGGVVTNLAGKPPQTAGPVIAAANAALHAAALDVLTRSP"
     gene            complement(23918..25492)
                     /locus_tag="PL152_01260"
     CDS             complement(23918..25492)
                     /locus_tag="PL152_01260"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HQR47929.1"
                     /translation="MSQPHVLAIDLGTSGPKAAVVSLHGRIVATSHERVATHFIPGSG
                     VEQDPAEIWSAVKAACARALRSSGVAAQVRAVICASQYSSIVPVDAAGRATMNMILWL
                     DHRGETSRLKRLSGFPRAVDTPWRMLRWLRIHGLPPVEGGMSLTHMRWVRYARPDVYA
                     RTAKFLEPMDYLALRFSGRAVATQCTAFMSLLTDNRTIGTTRYDRSLLRYSCIDADRL
                     PEVVPLDSVIGEVLPDVASELGLPSGAVVVTGLNDTQAGGMGTAAFQGRHAAISIGSS
                     AVMITHVPFKRTDVRRAVLSMPSPVPDTWFVMAENGTGGAALDHFLGKVLGASGAVDA
                     PMMGDAYARIDRAVADTPAGSHGVLFLPWMTGSLAPCAERHMRGGFLNLGLDTTSDHM
                     ARAVMEGVAYNLRWLREAVQRFARREFESFTLYGGGAQSDTWSQIMADVLQVPVRQAH
                     EPRYVTCRGAALLAFQRLGLLGFADFAPLIPTRREFEPDTSTSSLHAHRHAQFVQAFR
                     RNRPLFRALNGQEGPA"
BASE COUNT         4104 a         8428 c         8618 g         4070 t
ORIGIN      
        1 atctcgacgt tgagcagcac gatgtcgatg tcgaagaaga agtacaggtc gacgtgcacg
       61 agcgacaacg tcaccggctc gctgcccgcc tcggggacga gacgcactgc agccacgtcg
      121 tcacggcgga atacgcgcat cggcgacgcg cccgggtccg cggggccgcc gacccgtgcg
      181 cgctgctcgc gcccctcgcc gtagaggaaa cgctgcacgt gcggcaggaa cgtcgtgaac
      241 tcggtgtaat ggcgctcctg gaaccgggcg ggatcaccag tgaattcgtc ggcgacctcg
      301 tgccacggat tgtccgggcc gggctgctcg agcaactccc aatgccgctg gatctgcatg
      361 ccgtgacgaa tgggcagcag ttgcagcggc cacagcagga tctgccggaa ctggcgaacg
      421 agcttgtcat gggcagaggg ttgcatcgtc gctcccgccg gtgcgtccgg cgcccatgat
      481 ggcgtatcgt gcccgctcgt caatgcgtcg gcggggcgcg cgattccgcc aaatgacagg
      541 acgttgcggt ccatgagtga atcggagcca ggtctgtccg tcgccacgtg ggtcctcgca
      601 ctcggcgggg caggcttcgc cgcgggcttt ttcggtccca tggcgctcaa tcccggcgcg
      661 aaccagggtc cgatggccgg catcttcatc acgggtcccg gcggcgcgct gctcgggctc
      721 gtgttgggcg tggcgtcacg gctcctcgga gcgggcctcg cacgacagcg ccaagccctc
      781 atctccgcgg ccgccgtgtt gtcgctgggg accctctacg catgtctccc ggagcccgag
      841 accgagggcg tactgatcga agggtccgtt cagacgtgcc agccgcccgc ggaactcgag
      901 gaggctgcga tccaggactg gcagcgccgc atcgaagccg caccgtgggg acagccgcgc
      961 gacggttggc gcgagggcgt cgaccggatg cttcgggacg cggacggcgt cgtgctcacc
     1021 gtcgacgtgc aacgcgagga ccggatcctg cggcatcgca agccgtggaa cgccggtcgg
     1081 aagaccgttg caggctggac gcaggcgaat gaggctcggc gctactacgc agcattcgcc
     1141 ggccgcgact gcgcgtccta cagcggtccg atccagatcc tgtacgtccg ctacggccag
     1201 ggatcgggcg tctggccgcc ggacgacgcg ccgaatttcc tgggcgtggc gcgggtcgag
     1261 ccagccccag cggcgctgct ggcgctgaca caacactgaa tcggttgtcc cgacgggaga
     1321 tcagcatgac gaagccggtc atcaacgtgt ccgatgccan nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnt tgtcccgacg ggagatcagc atgacgaagc cggtcatcaa
     1501 cgtgtccgat gccaaggtcc atagatcgtc ggctacccgg actcaggcaa gaccggcgtg
     1561 cggatcgcgg ccgacatgac tcctgagtcg cggtacgtca tcagcgacac ggccaggaat
     1621 gccgtcggct actgggaggg tgaggactgc gccaaggtcc tggacatcgt ggaccgggcc
     1681 cggggccgcc gctgacgatt cagctgctgg tcaagccctc gacgatcact gcgttgccgg
     1741 tcgatgcgcg gaaccggtgc tgggcgatgg cctggtactc cggcgattgg taccagcgcg
     1801 cggcttcctc ctgcgaaggg aagcgaatca ggacggtgcg cgtgcagggc cactggcctt
     1861 cgacgaccgt ggggttctcc gagacggcca gcaactcgcc cttgaacagc tggaagacgc
     1921 cgaggaagcc ttcctcgtat cgggaatatc cggcgcgatc gtgaatcgtg atctgtgcaa
     1981 cgacgtaaac ggacatggtg tggtctcaat tgaacgaatg cgacgcagcg acggttcgct
     2041 gcgtcgcgat actagccgac ctggtgccac cagtccgcgc ttcagcggaa tgtcttgcag
     2101 tccgcgatga acgaacgacg cgccgcgccg acgagatggt cggtgtcggc cagctcttcg
     2161 caggccgcct gtcgacccgc catgccattt agagcgatgg tcgacgacct cacgcattca
     2221 gtcacgaacg tatgttgcgc gtcgccgccg atgtgtcttg cggcggcgag cagctgacag
     2281 gtcgtggtct ggtccggcgc ggtgctggca tatgcgggtg gcgccagtgc agaagcaatg
     2341 acgagagcac tggccgccgt gagctcatgc agtctcatgg caggttcccc ctgtgttgca
     2401 gtgtccagtt cgcttatacg ccgagtacgc gtcatgggta tcgctgcacc gaaaatcact
     2461 gcaatgcaaa atcgtcgccc cggtttcggc ctcggcgttc gagtcgcaaa gactcgcgcc
     2521 gggcgtggcc gtgcagcctc ccggcggtaa actggcgcct ggacggcccg gctcgggtgc
     2581 gtcggcaggc gggaggtacc acgatggccg tgcgaatcgt ccggctcggc agtcaacgcc
     2641 tggcggggga gggactgcgc atcggaaccg tgcggcgtcc gccccgcggc gtgccgaaga
     2701 gcgactttgc gaagcgcgat ttctacgacg tctggctgcc caacctggcc ccgagcgagg
     2761 aactgctgaa ggcggcgcac gcggcgatcg gagctgacga cgagcgggcg tggaaggcat
     2821 tcgtgcgacg ctaccgggca gagatgaagc gccctgacag ccgggcgctg ctccagacgc
     2881 tcgcggccct gtcgcacacc gcggatttct ccgtcggctg ctactgtgcc gacgaggcgc
     2941 gctgtcacag gtcggtgctg cgtgaactgc tggccgagca cggcgccagg ctcgcgtagg
     3001 cgaccacgcg atgcaggggg attcgatgcg acacgatggt cgagtgttgt cgaggaaggc
     3061 gcggagcagt ccgagtcggc tcgcgcccac cctcacgctg ctggtcgtcg cggcggccgg
     3121 cggctgcggt ggaggaggtg gtggcggtgg gggcggcggc tccggtcctg ccccggacac
     3181 cacgccgccc accgtcccgc aaggagtgac cgcgacggca agctcctcca ctgcgatcgt
     3241 cgtcaactgg caggcgtcca ccgatgcagg tcgtggcgtc gcgggctacc gcgtcttccg
     3301 ggacggtgcc gcgacggcgg tggcgacggt gaccggcacg acgtacgccg acacggggct
     3361 cacggcgtcg acgcagtact cctacgcggt tcgggccttc gacgcagcca gcccgtccaa
     3421 cgagtcggcg ctctcgacct cggcggccgc ccgcacgctc gatgccagcg tgacgtcagg
     3481 gctcgatgca cggcccagca acacgacgtg cctcgccggc gatcggcctg cgcagaacgc
     3541 cgcgcttgcg gtcaaccgag tgttccccgg tgtatcgttc gcgaatccga tactgatgct
     3601 cctggcgccg ggggatgcaa cgcgctggtt cgtcgtggag cagggcggcc gggtccgcgt
     3661 attcgacaac caggcgtccg tctcgacgac ccgaagcttc gtggacatta cagcgcgggt
     3721 gcagtccggc ggcgagacgg gcctgctcgg gatggcattc cacccgaatt tcccgacgaa
     3781 tccgcgcgtc tacctctcgt acacgaacgg gagcagcggc ctcgtgtctc gcgtgtcgga
     3841 gtttcgcagc accgatggcg gccagaccct cgatccgacg accgaggtga tcctgctcac
     3901 cgtcacccag ccggaatcga atcacaacgg cggcaacatc gccttcggcc cggacggata
     3961 cctgtacatc gggttcggcg acggcggagg tggcggcgat cagcaccctc cgatcggcaa
     4021 tggccagaac ctgacgacga cactagggaa gctgctgcgc atcgacatcg gtggttccac
     4081 cggcagcgtg ccgtaccgca tcccggccga taatccttac gtgggcaatg cgctctgcag
     4141 cacgggatcg ggcgcgcaga actgcccgga aatctatgcc tacgggttca ggaatccctg
     4201 gcgctggagc ttcgatcggg tgagcggcga actgtgggtc ggcgatgtcg ggcagaatct
     4261 gatcgaggag gtggaccgcg tcgttgccgg cggaaactac ggctggcgct gcttcgaggg
     4321 caccagcacc tacaactcca actgcggccc gaacgcgggc agcagcctgc cgcccgtcgc
     4381 ccaatacgat cactcggtcg ggagctcgat cacgggcggc tacgtgtatc gcggttccgc
     4441 cattcccgcg ctggcagggc gttacgtgtt cggcgacttt gggtcgggtc gtatctggaa
     4501 tatcgcccgc gacacggcgc ccacgctcac ggtcaccggc acacccgcct ttgccagcgg
     4561 ccgatccatc tcgtcctttg cgcaggacag cgccggtgag ctgtacatcg tggactacgg
     4621 cggcgcgctg taccgcctgg acaacgccgc ggcgggcggc ggttccgtac ccgcgaaatt
     4681 gtcggacagt ggctgtgtgc agcccggcga cccgacgaag cctgcggcag gactcgttcc
     4741 gtatgcgccg aacgcggcat tctggtcgga tggcgcgagc aaggaccgtt atgtggcgct
     4801 gccgaacggc acatcggcga cggtgggcac ggatggggat atggatctac nnnnnnnnnn
     4861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gcgctgccga acggcacatc ggcgacggtg
     4981 ggcacggatg gggatatgga tctacctgtt ggcaccgtgc tcatgaagga attccgcctc
     5041 ggaagccggc gcgtcgagac gcgattcctg atgcggcaca ccgacggcga atgggccggc
     5101 tacacctacg agtggaacgc acagggtacg gacgcgaccc gcgtcgtcgg cggcaagacg
     5161 gccgtgatcg gcacgcagac ctgggtcttt ccgagcgagg cgcagtgcct gtcctgtcac
     5221 acctccgcgg ccgggcgcac gttgggcctc gagctcgccc aactgaacgg cttgctcctg
     5281 tacccgcaga ccggtcgcac ggcaaaccag gtcgtcacgt ggaatgcgat cggactcttg
     5341 aatccgcccg tcgggggcgc gcccgatcag tgggcctcgc tgcccgatcc gtacggaagc
     5401 gccggcacgc tcgccgaacg cgcacgcgcg tatctgcact cgaactgctc gcagtgtcat
     5461 cgcccaggcg gcggaacacc gtccgacatg gacctgcgct atgcgacgcc gctcgcctcc
     5521 acgaatgcct gcgaccggcc accccaggca ggagacctcg gcatcgcgaa cgcccggata
     5581 atcgctccgg gctcggccac gcgatcggtg ctcgtctcgc gcgtcggtcg gcgcgacgcg
     5641 caagcgatgc cgccgctcgc tagcaccgtg gtcgatgcgg caggcgtcag cttgctcacc
     5701 aactgggtta actccctcgc cagttgcaac tgatcacgcg gctcgcccca tgctgaaatt
     5761 cctgctgtgg ctgctgctgt tcgtcgtgtg ctggccgctc gcgatcgcag cgctggtgct
     5821 ctggccgatc gtgtggctgt tgtcgctgcc gttccggctg gtcggaatcg ccgtcgaggg
     5881 tatgttcgcg ttcctgcgcg cgctcgtgat gctgccggca cgggttctcg gcagccggcc
     5941 ggcgctgagg gagtgaattc cgatcgcgct cggcgccggt cgtcaccacc gcccatgtgg
     6001 atcgcgagcc gcgtgggtcg cgtcgtgtcc cgcatgcggg tgcgtaccga gcgggtcggc
     6061 ccccggcggt cggacgacga agcgacgcac gcggctgtgc tcgctgacga tcagtcgtcc
     6121 caggcgtgcg cgcacggcct ggtcggattc ggtcggtcgt gtccgccagc gcaggtggtc
     6181 catccacgtc gggctctcga agcgttccac ccagaggtcc ggcgcgtcga tgtcctggca
     6241 gatcgaccac gcacgggccc cgtcacgtcg ccggatgcgg ccgacctcgt tgatcacggc
     6301 gacgaactcc accgcgtgct cggccgggac ccgatactcg atggagacca cgatcgggcc
     6361 agacgacgga tgaatgtcgg cgcttggtgg attgcgctcg atccggaccc gcgggtcgag
     6421 cgagcccagc tgctcgacgg cgatcggcag ccagcgcgcc gcgaccaggg acaccagcgc
     6481 ggagaccccg gcgacgacca gtgactcgcg caggcccgcg tcagcggcga accggcccca
     6541 ccaccagctg ccgagcgcga tgctggcaaa tgcgaccgtc tggtagaccg cgagcatcct
     6601 cgccgtcacc cagcgcggtg aggacagctg cacggcgatg ttgaaattgg cgaacgccag
     6661 cgtccacgcc gtccccgcaa ggacgtgcat gagcaacgtg agcgccatcc agcggctgag
     6721 tcccgcgccg accatcgcga cgcaggcgag cgccgacagc agccgcagca gaccgtcgtg
     6781 cgtgaactgg ctgcgcagcg gcgcactcgc gagcgcgccg agcatggcgc cgacgccgaa
     6841 gccaccgagc agcaagccgt aggtggtggc gccaccgccg agcaggtccc gggccacgat
     6901 cggcatcagg gccggcaccg ccgcgatcgg gatcgtattg agcacgcagc gcagcatgat
     6961 cgagagcagg tgtggggaca ggctgatgta gcggacgccg gccgcaatgg ccgtgccgag
     7021 cggctccggg ggcagttcgc tgtgggctcg tgccgggcgc caccagagca aggtcgcgat
     7081 gatgccgagg taggagaccg cgttgatgat gaacgcggcc gccgcgctga aggccgcgac
     7141 gatgactcca ccgatcgccg ggccgaggct gcgggccagg ttgaagccca ccgcattggc
     7201 catgaccgcc ggtgcaatct gtgcgcgcgg cacctggtca ccgatggacg cctgccatgc
     7261 cggggcgaac agcgcgcccc cgcagccgat caggaaggtg agcgtcagca gcagcgacgg
     7321 cgtgatgcgg ccagccagca ccaccacggc cagtgccacc gatgcgaaca cggcaaggat
     7381 ctgtgatatg agcatgatcg cgcggcgatc gcgcgtatcc gccagggcac ctgccaccag
     7441 cgacaggaag aagaagggca gggaaccggc ggtctgcacc agcgcgacct ggtcgacgga
     7501 cggagcgatc gtggtcatca gccacgaggc accgacggtc tgaatgaggc tgccgaagga
     7561 ggatacgagc gaggcccacc agaacagcgc gaacacgcga tgctcgaacg gcgcgagcgt
     7621 catgctgcgc cagcgttcga agcgagtcgg gttgacagtg gccnnnnnnn nnnnnnnnnn
     7681 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7741 nnnnnnnnnn nnnnnnnnnn nnncggcgcg agcgtcatgc tgcgccagcg ttcgaagcga
     7801 gtcgggttga cagtggcccc gctgcccatg aatcaatcta gcatgaagcg aatatagggg
     7861 tttccgaggg tcgggcaggg cattggctgc tgtagtgttg atggttgcgc ggtcgcgaac
     7921 gggtgtcgca catgaagacg ctgagcgggc tcgacgggat gttcctgcat ctcgagacgc
     7981 cggagacccc gatgcatgtc gggtcgttga tcgtgctgca actgccgaag ggttaccgcg
     8041 gcgactttct cgcggacgtg aagcgtctgt acgcgcgccg cgcggcgctc gcgcccgtgc
     8101 tcaagcgaac gctgcgggaa ctgccgctgc agctggccaa tcccgcctgg gtgcaggcgg
     8161 acacgctcga tctcgattat cacttccggc gcgtggtgct gccgaaaccc ggcacgcagg
     8221 cgcagctcga ggcctgtgtc gggcacctgc actccgtgcc gctcgaccgc gcgcatccgc
     8281 tctggagcgt gacgatcatc gagggtctgc ccggtcgaaa ggtcggttac tacacgaata
     8341 tccaccacgc ggtcatcgac ggccaatcgg gggtcgaact cgcgaaggtg gtgttcgacc
     8401 agacgccccg gccgcggcgg atgccgcgca tgctcgcgga tgcagcgcac ggcgagcacc
     8461 cgtggccggg ggcagccgtt gccggtgcgc tgcggcacga tgccgcccag ctcgccaagc
     8521 tggtgcagca cctgccggag atcacgcgga cgatcgctgc gatgcgccgc gccgccagtc
     8581 gcgtcggcac ggaggagccg gcgcggaccg agcgtcggtt cgccccacgc acgacgttca
     8641 acgccgcaat tcgcgccgcg cggggcttcg ccggcgtgtc gattccactg ggcgaggtcc
     8701 accgcattgc cgctgcacac cgggccacgg tgaatgacgt ggtgctcgcg atctgcgcag
     8761 gcgcgctgcg tcggtatctt ggccgccacg gcggcgtgcc gctggacagc ctgatcgccg
     8821 ccgtgccgat ctcgctgcgg gaagccggta acgtcgagta caccacgctc gcgaccatgg
     8881 ctcgggtgag tctggccacc gacgtcgccg accccgtgcg gcggctgcac gcgattcggg
     8941 acgcggcgag cgcggcgaag gccgcgacgg ggcgggccag gtcgatcctg ccgacggact
     9001 tcccgacgct cgggctgccg tggctgctgc acgggctggc cacgctgtat ggcgccgcgc
     9061 acctcgccga tgttgtgccg ccattgttca acctggtgat ctcgaacgtg cgcggtccgc
     9121 ccatgccgct gtacatcgcg ggcgcgcgcg tcgtgcgtta ctggccgctg tcgatcgtcg
     9181 cccaggggct ggggatcaac atcacggtcg agagctacgc gggctcgctc gagttcggcg
     9241 tgaccacggc ggcgagcgca gtccgcaatc cgcgcgcact gggtgcggcg ctgctggcgg
     9301 cgcatcggca gttgctcgcg cgaacaattc gaggtcgccc cgggcgtggg aagacgcgga
     9361 cgcgggcggt gcgaggactc ggttgatcgg tccacttatc ccgttcgtgg cgatggcctg
     9421 acgattggat ctcgaggcta gcgcgtctaa cggggtgtcg tgcagcactg cggaggatcg
     9481 tcatgggcaa cacactgcgt gggttgcggc acaaaccgca attaccaggt ctcgccgcca
     9541 gcctgcgaac actcaacgcg tggatgcggg cgttggtgat acagaccgcg gcggtgcaag
     9601 cggtctggtc ggccgctctg caacgcaatc tcgaccgcgg tcgtcgcatt tgacgccacc
     9661 ggcgcccgtc ggcgggcgac atttcaagcc gtgcccgtgc cttccgacgg atttccccga
     9721 gctggcgtcc ggcggtcttc gggtgccaaa ggggtagcta aggtcgccgg accagaagct
     9781 cgattgcggg aatcgaattc aagatattac ataatataca ttatgcgtac cgtggtggtc
     9841 ggcggcctag accggatgtc gcggcattct cgtgaccagc ccagcgaacg catgggcaac
     9901 agacaccctc ccgtaagctg ccgcagcaca tgcacgaccg agtggaattc agcggtccac
     9961 ggcgcacccg actccgtcga atcaaggaat acggtcggat tccgaactcg ccccgagcct
    10021 ggggtgtcgc ttcgcggcag tgagcaggaa gcccaggctg ctgaccgtgc cgatacccat
    10081 cagcgtcatg cgcaggtaca ccgcgggcca cggaatggcg atgtcgacgc cgcgccggtt
    10141 gagttcttcg atccaatgct gcccgaccgt accgtcgtac atccagcgca atatgaggtg
    10201 ggctgaagcc agcgcgtaga gaagcgccgc gagccatcgg aggccccaac gcaatcggtc
    10261 tcccgccacg aaggcggccg ccaagacagc gaacgtgatg gtgagccaga actgaaactg
    10321 caggtcgatc gagctacgct gcgcaagcag cagattgagc aggtccgccg ttgacacttg
    10381 gtccatcgtc agctgtcgcc tctgtgcccc gatgaatccc gcaccgacac cagtcgcata
    10441 gtctcacgtc acggactgca ggcgtgtgtc gccgtcttgc tccggcaggt caaccgccgc
    10501 gctgccttca gtgctggcag tgctggcggc ccggcctaaa aacaggtacc ttgctcaaca
    10561 aaggtaccgc attcatcgtc cgcgcatgac tgacgaatcg aagctcgcgc cgctgcgtaa
    10621 aggactgctg gagttcctgg tgctcaagat cgtgggtgcc gagcgcatgt acgtgccgga
    10681 catcctgcgc aggctcgccg ggaccgagtt cagcacgcag gaaggcacgc tctatccgct
    10741 gctgagcaag ctgcgccggg aggatctggt gcaatatgag tggaaggaat cggacgccgg
    10801 gccgccgcgg aagtactacc gcctgaccgc ggccggccgt atgcagctca aggcactcga
    10861 cgagtattgg caacagatca accgcacagt cgcatccatc ggacactgag ccatgcaacg
    10921 catttccatc accgtccgtc tcaatcgcag cacgcttcaa ttcgacgagg cggcattcgc
    10981 gcgtctcgag caatacctgg cggagtcggc aggtctgctc gagggcgatc ccgatccgca
    11041 ggagatcctg ggtgacctcg agcaggcggt ggccgaccaa tgcacgcgtc gcctgccgcc
    11101 ggagcgcagc gtggtgacgc tggcggagct cgagccggcg ctcgccgaga tcgggacggt
    11161 gcaggtgccg ggtacggcat cgacggctca ggaccggccc gcccctgcgg cctcgcggcc
    11221 gcttcagcag atcagcgaag gcgccgtgat cagtggggtg tgccagggac tcgcgcggta
    11281 cttcggactc aacgtaacgg cgctgcgcgt gttcgccgtg ctgctgctgc tgctgtccgg
    11341 cggcggcgtg attgtcgtct acctcgcact gatgctgctc atgccatacg cgccgctccg
    11401 gagtgacggt ccgtcgctca gctggctgcc ggagaagtgc cgcacgttga cggagttcct
    11461 gcgcgcgaag tttcacctcg cgacgagctg acttccccgg ccacgacagg gcaaggcgac
    11521 cgagggtggc cgccctgccc cgtgcaagca acggccgtgc gctcaggcgg tcgcagcgtc
    11581 cttcagtttc ttcatcgggc gcaccttgac cttcacggtg gccggcttcg ccttgaagac
    11641 ggtctcctga cccgtgaatg ggttgatgcc cttgcgcgcc ttcttcgcgg gcaccgccac
    11701 cgcggtgatc ttgagaatgc cgggcagcac gaacgtgcgc gggcccttct tgctgatcga
    11761 ggcgtggatc gtgtgctcga gtgcgtggat caccgcgcgc acgtccttcg cagcggcgcc
    11821 agagacgccg gcgaggtgct tcacgagcgc cgacttgctg agcacggtct tgacggcctt
    11881 cggcgcggct ttcgcagcag ccttcggggc ggacttcttc ggggacttct tggccttggc
    11941 catgacggag ggtgacctct tcgtgaatgc tgcgatcgac gttgatcgcc gcgcgcacgt
    12001 taggggaaat ggcgcggaaa gccaagcggc gtggatgcgg gcgtcaggcc cgatcgaccc
    12061 ggcaggcaaa gcagccgggc cgggcattgt ggacatgcag ataggccacg tcgtcccggc
    12121 cgaagtagcg atggatcaat gactcgagtt cgttgcccgg ggtgacatcc gctgcgacga
    12181 tccagttgcg atcgtcgtag gcacggaccg agagcagccg gcgacgctgc tggtcaggga
    12241 tcgtgtcgcg cgcaatgcgc gtagccgagg ccttgcgtcg gatgaaaatc gggccggcgg
    12301 cggcgtatgg cgtcggaccg ggctggtgct ggtagctcag gagcaacagc tcctcgccgg
    12361 gggccgcgtc ttccagcgag atccggcagg gatagccgtc ggcggagtcc gcggtgacca
    12421 cggagacacc gacagcggcc agtgcggcgg agtcgagcgc gagcaggtgc gagaaccggg
    12481 cgatgggcag gccgcggatc gtgaatgtca tcggaaggct ccctggacgc cgtcgaacgg
    12541 cgatgagcag ggagactgcg gcggctggcc gggcccgtcg acccgaatcg tgctccgagc
    12601 gttcagtgag gccgggatcg atagcggaac accggccgcg ttaccggcga cgcccggccg
    12661 ggtcgcgcgg gcaatgcgcg cccggcctgg agttgcagct cagatgaacg actgcgcggc
    12721 gacgatggcg tgagtcgcgg cagccatcac cacggtgacg gcaatcgtga gtccggcgag
    12781 cgcgacgtcg aggaagcgat ccgaagcctg gcgggcgaac atggcgtgat cctccgttcg
    12841 gtacgggggc caaggtacgc gcgaagactg gcgcaataaa ctgccttaat ccgatcactc
    12901 tgttcagcat tgcaggacaa tcgcttccgg catgggcgcg ggccgcgccg ttcggcgacg
    12961 tgccagaggc cgtcctgacg ataattgcgc tgcgaggctg cgggccagga cgtgaagatc
    13021 accatcgata acatgcggcg ctacgccgtc gcgcgctcat tgtttcgccc gaccacgctc
    13081 gggcgtgcaa tccggcggct cgggttcgtc caggccgatc ccatccgcgc accggcccga
    13141 gcccaggacc tcacgctgcg gcaccgggtg gtcgattacc gcgcgggcga cctcgagcga
    13201 cgctatgcgc gtctcgggct cgaggaggat ttcttcgtca actacggttt cctgcggcgc
    13261 gagcacagcg cgctcatgca cccgcgcacg ccacggcggc cgtggtccgc cgaacgacgg
    13321 cggcaggccg aggcggtgct cgaattcgtg cgccagcgcc ggtcggtgca cccgcgcgag
    13381 gtcgatgcgg ccttcgcgca cggcaagacc aggaactggt tcggcggctc ctcgaatgcg
    13441 agcacgcaac tgctcgacga gatgcactac cgcgggctgc tgcgggtggt ccggcgcgaa
    13501 ggtggcactc gtgtgtacgc ggttcgctcg catactgcgc cgtcggcctc ggacgacacc
    13561 gcggtcgcga cgcgcatgga tgagctcgtc gacctcatcg cgatgaagta cgcaccgctg
    13621 ccggcacgca gcctcggtta ctacctgagc ctgctctgca caggcgtgcc gcagtggcgt
    13681 tcacgacggg cggacgcgct cgcgcgtgcc cggcagcggc tgggacgcgc gcgggttgga
    13741 gccgaggatt ggttctggcc ggccgacgag aatccgggct cccggcgctg gcgtcccgac
    13801 ggagcggtgc gattgctcac accgttcgac ccgatcgtgc acgatcgaca tcgattcgag
    13861 cgcctgtggg actggcgcta ccggttcgag gcctatacgc cgccgtcgaa gcggtccctc
    13921 ggctattacg ccctgccgct gctctggggt catcgggtgc tgggctgggg caacctgtcg
    13981 gtcgtggatg gcgcactcag gagcgagttc ggttatgtcg gcggagttgc gcccggtgat
    14041 ccaggattcc agtccgaatt gcgggcggag ctggagcgaa tgcaggtctt cctcgggatc
    14101 aacgggtgag catcgccggc tccggcgctg cgccaacgcc gtgcgcaggc agccccgttc
    14161 agtatcattc acgcatgaca gcgcgcgccg cccttctcgc gacactggtc cttgcggcct
    14221 tgctcgcggc ggtaccgtca tgggccggct atacgtacgt gcctgcgcct ggactcccgg
    14281 cgtcggacct ggttcagccg cccgagcttg cagccctgtt ggcggacccg tctgcgcgca
    14341 agccgctcgt gctgcaggtc ggagcacatg tcctctacgt gcaggcccac atccctggct
    14401 cggagtacgt cggccccggc gaagacgacg cgggcctcgc ggcgttgcgc agccgggtcg
    14461 cccggttgcc gcgcgatgcg tcgatcgtca tctattgcgg ctgctgcccg tgggaccact
    14521 gtcgcaacat cggtggcgcc tatgcgcagc tgaagcggat ggggttcacc cgcgtcaagg
    14581 tcctctacat tgcgtcgaat ttcgtcagcg attgggtcga tgcaggctat gcagtggcga
    14641 cgggcgactg acctggtgag gcagcgggtc ggcgcggact tgcattggcg agccgttgcc
    14701 ccaacagttg cgccctccgg cacggcgaac cgccccgcag catgaggacg catgaacgca
    14761 ttcggcaaga aacctgacgg cctgcggctc gagcgcatgc aggcgtcgcc gctgtggcac
    14821 gggggcacgt tccgcaacat gcatcccctg ctgccgcgcc tgctcaagcc gaacgtcgca
    14881 gcgccgacgg tcagcgcgtt cctgttcgaa ggcgcgcgcc gcgttccgac ggcaccgctg
    14941 ccggcggtcg atccgaccgg cgcgtggcgc aggaagcccg gcagcggcct gcgcgcgacc
    15001 tggctcggtc actcgacggt gctgctcgag atcgatggcc tgcgggtgct caccgatccg
    15061 gtctggagct tgcgcgcctc gccatcgcag tgggtcggcc cgaagcggtt ccagcccgtg
    15121 ccggtgtcac tgcgctcgct gccgcacctc gacgcggtcg tcatttccca cgatcactac
    15181 gaccacctcg actatcgggc aatccgcgag ctcgcgcgac gtgaggtgcc gttcgtcacc
    15241 tcactcggtg tgggtgcgca cctcgaggac tggggtgtgc cgccgaagcg cattcacgaa
    15301 ctcgactggt gggagtccta cgcgctgccg agggccgatc tctcggtgac ggctgcaccg
    15361 tcgcagcatt tctccggtcg cggcctgcgc tcccgcaact cgacgctgtg gtcctcgatg
    15421 gtgatccgct cgcagcgcca ttccgtgttc ttcagcggcg atacgggcct cacgaccgag
    15481 tacgcggaga tccgtcggcg actgggtccg ttcgacctgg tgatgctcga agtgggtgcc
    15541 tatcacccgg cctgggggca catccacctc ggcccggaca atgcgctcga ggcgctggac
    15601 ctgctcggtg gcggggcatt tctcccggtg cactggggaa cgttcagcct cgcgatgcac
    15661 gcctgggacg atccggcgga gacgctgttc gccgaggctc ccaggcgtgg cgtccagctc
    15721 gtgatgccgc gcctcgggga agcggtcgaa ccggcgcacg tggacagggt gaagccgtgg
    15781 tggagatcgg tggagcaggc gcagcgccgg gcacccaaag ggttgcagga gcctcgtgac
    15841 acgccgcctc cgaagtccat gccctggccc atcgactgag tgccgccgca cgatgcagac
    15901 ggtgctcacg attcgcaccc agggaccggg actgtacgaa ttcaccgccg acgtcgcacg
    15961 cttcgtgcat gccagtggcg tgcgcgaggg gctggtgacg ctgttcgtgc agcacacctc
    16021 gtgctcgctg tgcgtccagg agaatgcgga cccggacgtg cgacgggatc tcgacgagtt
    16081 ctttcggcgc ctcgtgccct cctccgatga cccctcgatg cgatggatcg cgcacaccac
    16141 cgagggaccg gacgacatgc cggcgcacat caaggcggcg ctcaatgccg tgtcgcttgc
    16201 ggttccagtg accgatggac gcctggcgct cggtacctgg caggggatct acctcttcga
    16261 gcaccgagat cgcgggcaag cgcgccgggt gtttgcgcac ttgactgcgt gaaagcgccg
    16321 ctcgcggtca cagtccgtcg cggactagaa tcggctgacc ggtcgtgaac cacgccgtcg
    16381 cgaggcattg attcccatga agatcaccgt tcatcgactg acgcgtgcct gcgcccttct
    16441 cctcggcttc gcggtgtgcg gcagcgtgag cgcgggggcc gggcaggagg cgactgctca
    16501 agcccagcac gagcacatgg ccggcggatc cgccgtcgcg aacatgaagc tcgacgacgg
    16561 gaagcaatgg gcgacggacg cgccactgcg cagcggcatg gcagcgatac gcaaggcgtt
    16621 tgatgccgat cacccggcga tccacgccgg cgaggagacc gatgcgcaat atgaagttct
    16681 cgcggggaag atcgaggcgc aggtgaatga catcgtgcgg aactgccacc tgccgccggc
    16741 agccgacgca aacctgcact ttgtcattgc ggacctcctg cagggcgtta acctgatgcg
    16801 cggtacgaat gcagcccgca gccggcacga cggcgccgcg ctggtgcacg gggcgctgat
    16861 cgcctacggg cagtatttcg aggatccaga ctggagacat tgagccagcg cgttgatggc
    16921 caccgtactc gtgccgctgc ccagtcggga cttcgacccg accgagacgg gtgtgccgtg
    16981 gaagtcgctc cgcgcgctcg gccatcgaat cgtatttgcg acgccggacg gccagcccgg
    17041 caccgctgac gaccgcatgc tgacgggccg ggggctcggc gtgctcgcga ggttcctgat
    17101 cgcggacgcc aatgggcgac aggcccatgc ggagatggcg gcgagcgagg aattccggcg
    17161 gccgatcacg cacaccaccg cccgcgtcgc cgactacgac ggcctgctgc tgcccggcgg
    17221 tcatgccccc gggatgcgga cgtacctcga gtcgaagcct ctgcaagcgc tgatcgtggc
    17281 ggcattcgac cgagggctgc ccgtcggcgc aatctgtcac ggagtcgtgc tggcagcacg
    17341 ttcacgtcgc aacgatggcc ggtcggtgct ctgcggccgc aagacgacgg cgctcacgaa
    17401 gcagatggaa ctcactgcct gggcgctcac ctgcacgtgg ctggggcgct actatcgaac
    17461 ctacccgatg accgtcgagg acgaggtgac ggccgcactc gcgaacccaa cggacttcct
    17521 gcgcgggccc ctgagcacgg cccgcgatcg acccgaggat ctcgccgctg gtttcaccgt
    17581 acgcgacggg aattatctat ccgcccgctg gccgggcgac gcgcatcgat tcgcgagcga
    17641 gttcgcgagg atgctgtaga cgggcggagg gacgctccgt ctcctcacgg ccgaccggcg
    17701 cgagtcagga cgaagtcagt cgctcggcca gcgcgatcgt cttgcgggcc tgcacgaggt
    17761 gcggcatgtc cagcatccgg ccgtccaggc tcaatgcgcc cgcgcccggg ttggcctcga
    17821 agagttcgac gatgcggcgc gcacgcgcga cctcctccgc ggtgggcgtg aaagccgaat
    17881 tgatgaccgc gacctggtcg ggatgaatcg ccagcttgcc ggcgaaaccg tcacgccggg
    17941 cgcgccggga cgactcggcg aggcccgtgg agtcgcgaaa gtccgcatgc aaggtgtcga
    18001 tcgcgggcac gccggctgcg gaggccgcaa acaggcacag cgatcgcgcg agctcgaagg
    18061 gtgcactcca gcgcccgtcc ggttccttgt tgtcgatcgc gccgacggcc gcggccaggt
    18121 cttcggcacc ccaggtgagc gccgcaagcc gcggccccgc gtcacgatac gtgccgagcg
    18181 tgaacagcgc ttccggcacc tcggtcgcca cgggcatgat gcgcgtcgcg ccagccccga
    18241 tgccgttctc cgtctccaga atgtccaggc ggttcgacag ccgcaccgtg tcgtccgccg
    18301 aggccgcctt cggcagcacg agcccgtccg gtcgccccgc catgaccgcc gcgaggtcct
    18361 cgtggcacag cggcgatccg aggggattga cgcgcacgaa gagcgagcag ccgcgtcgtg
    18421 cacgatccgc gcgcagggcc tcgcgcacga tcccgcgggc ggcggccttg cgagaatcgg
    18481 cgacggcatc ctcgagatcc aggatgacca cgtccgcccc gaccgtcatc gccttggtga
    18541 tcttcttctc gctgtcaccc ggcacgaaga gcagcgatcg gtacatcctc aagctccgac
    18601 cggcttgcgc agcatgagcg ccgttcgtct gcacaccccg accagttcat tccgctggtt
    18661 gaaggcccga tgctggaaca cgatgatgcc gttctggggc cgcgacttgc tgtcgcgcag
    18721 ttcgacgacc tcgctttcga cgtggacggt atcgccgtgg aacagcggcc tcgggaaacg
    18781 cacctcgtcc cagccaagat tgccgaccgt ggtgcccagc gtcgtgtccc cgacggagat
    18841 cccgaccatg agtccgagcg tgagcacgct gttgaccacg cgcgttccga actcgctgtg
    18901 ctcccggcaa tactcctcgt cgaggtggat cgcagcggga ttgtgcgtca gcgccgtgaa
    18961 gaacacgttg tccgcctcgg tgatggtgcg gcggatcgcg tggcggaaca cctgcccgac
    19021 gtggaactcc tcgaaataaa gtcccgccat ggacctcaga tctcccgctg ccacggcagc
    19081 cggccatcga cctcgagctg cagcgacatg ctgaggaaag tccggatgac gacgatgagc
    19141 cccagcacgg ccacgttctg gagcgtcggc gccactgcga ccgtgcggat gatgtcaccg
    19201 gccaccaaga actcgagtcc cagcaggatg ccgcgtccga ggtcctggcg gtagccacgg
    19261 tacgccccgt cgaattcggc tcgctgcgcc agcgcgaacc gcacggtggc gatcgtggct
    19321 ccgagcacca tgatcagcac gccgaatgca tccacggcgt cactgaccag ttccatcacg
    19381 tgctgataat cgctcatggg catggtcgat cccgcctagc cggccttcgc gaatttcgcg
    19441 cgcgtcgccg cgatgaacgc aggcatggcc gcctccctgc gcgcggcgat ggctcgggca
    19501 tgcggcagct cgccgagtcg ctgcagcagc gccttggccg caggcatctc ggccagcagg
    19561 tcgatgcccc acagtttcgc cccgacgccg gccgcaagat cggcgctgaa catgaacacg
    19621 atgtccgcaa gcgtgaacgt gtcgccggcc acgtacggcg tgaacttgcc gtgccgcttg
    19681 agcgtggcga atccggccag cagattctcg cgcgccatgg ccttgatggg ctccggcatc
    19741 ttgccgccga agaaggcctc cacgaagcag gagcgcgccg gaagctcgat gtagagctcg
    19801 atctccttca tgagggccct gaccaggccg cgtgcatagg ggtcggcagg gagcagcgga
    19861 cggccctggc ccgtgtcctc gaggtactcg aggatgacgc tcgcctcgtt caagtaaccc
    19921 tgtggggtct ccagcgccgg caccttgcct cgcgggctct tggccagcca ctccggcgac
    19981 tggtccggca tgaccagcac gtattcgtac ggcacgccct tctcgagcag ggccatctcg
    20041 accatgttca cgtagttgct gaccgggaaa ccatgcagct tgagcatcaa ccagtcctcc
    20101 actcggaaat catcagcgcc tgtactcgcc gtcgaacaat gtcatccacc gggtaccgtc
    20161 gcgggacatg tccatgcgca tcttcagcgt cccggcggtc gagagatcgt acgtcattct
    20221 ggcgtgcatc ccgggaccgc cgtgcgcgac gaccagctcg tccccgtcga accgtcccga
    20281 aaagacctcc ggcggcgcgc ccatgctgtc gaaccagtgc aggttgtagg tcgtcgtggc
    20341 cgggtcataa gtcatgacgc cgtgcccctg gtaagtgacg tggccatcgc gctgttgctc
    20401 atagtccgtg acgagcgcga atccgccgag ggccatgcgg tagtcgagcc gagcggtcgc
    20461 cgtgccaccg ttggggtccc actgcgaagg gtgcatcgtc tcctccccct tccacagccc
    20521 cgccagtcgc tcgagctggg catgccccgc cgtcggtcgt ggcatctcca tggtgctctc
    20581 ctcgatgaaa tgagtgtgcg tgcctgcgcc gcacggacgg tgtctcgtgc agcaccgggc
    20641 ccatgatcca gcacgtcagg cgcggaccat gatcttgaag ccgccccaga acatgcgctt
    20701 gccgtcgaat ggcatgttct tcgggtccat catgctggcc aggcgcggat cgcgcatcac
    20761 cttggcattg atgcgatcgc ggtcgcgccg cgaccggtac aggatccacg agaaccagac
    20821 gacctcccct ttcctgagct tgacgctctg cgggaaggag gtccatttcc ccggcttcac
    20881 gtcatcggcc atgcactcga cgtaatcgag cgcgccgtgc tcgcgccaca ccttgcccgc
    20941 gaggcgcgac atgcgccggt acgcggccac cttgcgcttc ggcaccggaa cgacgaatcc
    21001 gtccacgtaa ccaggcatgc tgctcctcct gcatggattg ctttgttctt ggtcgtgacc
    21061 ggcgccgaaa cacgcggtca ggcatccata gggtactcgt attcgacgaa cccgctgtac
    21121 ctggcgacgc ggtcgtacag catccgggcc gtcgcattgg tctcgtgggt gagccaatac
    21181 aggcgccccg cgccgtccgc cctcgcctgt tgcgcgatgg cctcgatcag tgcccgggcc
    21241 acaccctggc cgcgcgccgc cggcgcgacg aagagatcct ccaggtagca cgctcgcggc
    21301 gcccagatgc ccgtgtgata caggtaatgc gcgatgccca ccagccggcc gtccaggcgc
    21361 gcaccgagcc cgcggatccc gtcgccggcc gtgagtcgtc gccaggtgcc gtcgtacgcg
    21421 gtgtccggca ggacggtctg gtagaagtcc ttgtaggcgc gtgcaagggg ctcccactcg
    21481 ccgcgctcgg acgcacccag gggcctgatc aggaggctca taagggacag tccgtgaatg
    21541 cgtccatggc gtgcgtgaag aacgcctgcg cgacggctgg gtgtgacagg tccatgtcga
    21601 ggccgccgcc gtagataccg ctgctcgcag gcttcgcgcg ggatgggtta cccgcatagg
    21661 cgactgcggc gtccaggtcc gcggcgaatt cctcgaccag gccgggacgg gtctgcgggc
    21721 ccgtgacgca catgtggatg gcgttcgggt gctgctggcc gttgaagcgc cagcnnnnnn
    21781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnncccgtg acgcacatgt ggatggcgtt
    21901 cgggtgctgc tggccgttga agcgccagcc gcgggtcttc atgaagtcgt tcacgtggta
    21961 gatgtcgaac gcgtccgaag tgaacgagaa gcagaacgtc ggccgtccca tgatgcgcag
    22021 ttccgggtgg gaccggaccg ctgcctgcat cgcgaacgcg gcgtcgaata tcttgcgcgc
    22081 ccgctccagg tagccatccc gcccgagcat catcatcgat gcccaggtcg ccgcgatcag
    22141 cccgccggac cggctgccgg ccagtcccgg cgacgcatac gccccgccct tccagtcggt
    22201 cagcatgtag tactggtgcc tgcgcaacga cttgtcctgc cacgccagca ccgacgtccc
    22261 cttgagtccg tagccgtact tgtgggtgtc cgccgaaatc gacgtcaccc cggggaggcg
    22321 gaagtcgaag accgggatgt cgggcacccc gagttcctgc ccccacggca ggatccagcc
    22381 gccgaggcat ccgtccacgt gcagtccgac acccgctgcc acggccacct cggacagttc
    22441 gctgatcgga tcgatcgtgc cgtagggata atttcccgcc gacgccacga tgaccgcggt
    22501 ctccggcgtg atcgagcgac gcacgaaatc cacgtcgacc tgcgtggtca cgggatcggt
    22561 cggagcgacg tcgacctcga gcccgaagag gtgcgccgcc ttgcggaacg ccggatgagc
    22621 cgtatccggc cagatcatgc gcggtcgcgt gattcgccgc tcggccctgg cccactcccg
    22681 ataggcgagc atcgcattga ggatgctctc cgtgccgccg aagcccagca cgccgcacgc
    22741 cttgcgcttc gcatcgtgcc ctgccacggc ctcgccgtgc agcatgtcga gcgtcatcgc
    22801 gacgatctcg ctctcgagcc ggttcatgct cgggcacatg tcccgctgca gcgcgttcac
    22861 gtgactgaag cttgcgaacg cctcgttcag cgccgcatag tgatcgtggt cgccgcagta
    22921 catcgttccg gaacacttgc cgctttccca ggtgcggtcc tcgcgctgcg ccatcgagcg
    22981 cagctgtccg agcacgcttg ccgtatccat gccgtgctgc ggcatccggc gcgtgacgcc
    23041 gaactcttcc gcgtatggat acagggcgcg cattgccttc tcgatgtcca tcgggcttcc
    23101 tcgtcgctcg tttccggggt tcagggcgaa cgcgtcagta cgtccagcgc ggcggcatgg
    23161 agggctgcat tggccgcagc gatgaccggg ccggccgtct gcggcggctt cccggcgagg
    23221 ttcgtgacca cgcctcccgc tcgctcgatg atcgggacca tggggaagaa gtcgtacggc
    23281 atcaggatcc cctcgacgac gaggtcgatg cagccgtgcg cgagcagcgc cagcgagtag
    23341 cagtcgccgc cccagcgctg caggcggcac tgcgcagcga ggcggtcgta ggccgcgcgc
    23401 agggccggat cgacgaacat ccgcggatgc gtgctgtaga ccgtcgcatc cgccatggtc
    23461 gcagtcgctc gggtccgcag ccgggtctcc cggtcgtgcg cccggtggac cgcgccaatc
    23521 cccggtgaag cgaggtacgt ctcgccggtg tacggctggt gcatgatgcc ggcgacggcg
    23581 cgcccgtcga cgaccagccc aagcaggatg ccccacgtgg gcatgccact cacgaaactc
    23641 gcagtcccgt cgatcgggtc gatgtaccag gcgacgggcc ccgagccgga ctcgccgtgc
    23701 tcctcgccga cgatgcgatg cgcggggtag cgctgcgcaa ggcgtccgcg gatcacggcc
    23761 tcgacgcggc ggtccgcctc ggtgaccgga tcgtagtgcc cgaacgtgga cttgtcgtga
    23821 gccacagggt ccttgcggaa atactggagc gcaaccggtc ctgcctcgag cacggccgcg
    23881 agaccgacct ccaggcactc cgagatctcc cggtcgttca tgcgggtccc tcctgcccat
    23941 tcagcgcccg gaacagcggt cggttgcgac ggaacgcctg cacgaactgg gcgtggcggt
    24001 gcgcgtgcag tgaagacgtc gaggtgtccg gttcgaattc gcggcgcgtc ggaatcagcg
    24061 gcgcgaaatc ggcgaacccc agcagcccga ggcgctggaa cgccagcaat gcggcgcctc
    24121 ggcaggtcac gtagcgcggt tcatgcgcct ggcgtaccgg gacctgcagg acgtccgcca
    24181 tgatctgcga ccaggtgtcg gactgtgcgc ccccgccata cagggtgaag ctctcgaact
    24241 cgcggcgtgc gaaccgctgc acggcctccc gcaaccagcg caggttgtag gccacgccct
    24301 ccatcaccgc gcgggccatg tggtcgctcg tcgtgtcgag gccgaggttc agaaagccgc
    24361 cacgcatgtg acgttcggca cagggtgcca gcgatccggt catccagggc aggaacagga
    24421 ctccatgact tccggccggc gtgtcggcga ccgcacgatc gatgcgcgcg tacgcatcgc
    24481 ccatcatggg agcgtcaact gcaccgctgg ccccgaggac cttgcccagg aagtgatcca
    24541 gcgcggcacc gccggtgccg ttctccgcca tgacgaacca ggtgtccggc accgggctcg
    24601 gcatgctgag caccgcgcga cgaacgtcgg tccgcttgaa tggcacgtgg gtgatcatca
    24661 ctgccgagct gccgatggag atcgcggcat ggcgcccctg gaaggcggcc gtccccatgc
    24721 caccggcctg cgtgtcgttg agcccggtga cgacgaccgc gccggagggc agcccgagtt
    24781 cacttgcgac atcgggcagc acttcgccga tgaccgagtc gaggggcacg acttccggca
    24841 gcctgtccgc gtcgatgcag gaataccgga gcagcgagcg gtcgtaacga gtcgtgccga
    24901 tggtccggtt gtcggtcagc agcgacatga acgccgtgca ctgggtggcc accgctcggc
    24961 ccgagaatcg cagggcgagg tagtccatcg gctcgaggaa cttggcggtc cttgcgtaca
    25021 cgtcgggtcg cgcatagcgc acccagcgca tgtgcgtcag ggacatcccg ccctcgacgg
    25081 gcggcaagcc gtggatgcgc agccaccgca acattcgcca cggcgtgtcg acggcacgcg
    25141 ggaagccgct cagtcgcttg agacgcgacg tttcgccgcg gtgatcgagc cagagaatca
    25201 tgttcatcgt cgcgcgaccg gccgcgtcga ccggaacgat ggacgagtac tggctcgcgc
    25261 agatcacggc ccgcacttgc gcggcgacgc cggaggaccg caaggcgcgc gcgcacgcgg
    25321 ccttcaccgc agaccagatc tcggccggat cctgctcgac accgctgccc gggatgaaat
    25381 gcgttgcgac ccgttcgtgg ctggtcgcga cgatccggcc gtgcagggac acgaccgcag
    25441 ccttggggcc gctggtgccc aggtcgatcg cgagcacgtg cggctggctc acggaggtcg
    25501 cgctcctcag gcgccgagca gcgcgtcggc gaagcggccc gccgcttccg gcgcatgcag
    25561 gaagaacagc gacggctcgg tgagttcgag ctcgagcacc acgggctcgc cacgaccatc
//
LOCUS       DAOMFD010000010        25142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_308_length_25142_cov_29.797704, whole genome shotgun sequence.
ACCESSION   DAOMFD010000010 DAOMFD010000000
VERSION     DAOMFD010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 25142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25142
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_308_length_25142_cov_29.797704"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="PL152_01265"
     CDS             <1..502
                     /locus_tag="PL152_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47930.1"
                     /translation="ERVEFIHEQTHSDALVEEYIEGREIYVGVLGNDRLTTFPLWELN
                     FGTLPEVMAGIATRKVKWDLKYQRKHGIGTGPARDLPEGMEHRIRQLVRRIYRALCLS
                     GYARIDLRLKPDGSVFVLEANANPNLAQTEDFATSALAASVGYDELLERIMKLGAAYD
                     VAWRQG"
     gene            572..2239
                     /locus_tag="PL152_01270"
     CDS             572..2239
                     /locus_tag="PL152_01270"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQR47931.1"
                     /translation="MGKVERSLRASLAALRIALSCTVALATAAVAGGADAGGAQAPAA
                     GAPAVDESTQAVFAVANRPIFTLRASLLGATPAERVAASEHRLDSIVKHGGPLVVSTR
                     DVDDGVALLVDGKFVVRVLTGDVDPESGESELSYAAAAAHNLRKALAEIREANDARAI
                     GRAVAYALAASVLLAVSCWVLLRAYRWLAARVHAAARRRLGHLLPAWADKVIGEAALG
                     GLLTVPFKLLVVVAIALLGYEWAGYVLQRFPYTRPWGEQLSDNLFEAAGRLLHSVLSA
                     IPGLLFVVFIFVAARILVRAVRAFFSGVEQGQIHVELVEKTTARPTGRLVTTAIWLFA
                     LVAAYPYIPGSGSEAFRGIGVFVGLMVSIGASGVVNQAVSGLMLMYTKALRPGEFVKV
                     GDVEGTVTSVGFVATRIETLRHEEVNVPNSVIATNVMHNYSRLSSERGLWLPATVTIG
                     YDAPWRQVHAMLTIAAERTEGVAHDPAPRVHQTALQDFYVEYTLLVNVAVPRHKREVL
                     TRLYANIQDVFNEHGVQIMSPNYEDDPESPKIVPKEKWYEPPAARPE"
     gene            complement(2251..2649)
                     /locus_tag="PL152_01275"
     CDS             complement(2251..2649)
                     /locus_tag="PL152_01275"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HQR47932.1"
                     /translation="MRGDLDPRQMEELKATMLERAKVLREEIRQTLLRSDSEQYAQVA
                     NEVRDLEDESFADLIVDVNLAEIDRDLEELRAVEAALLSVQEGSYGRCEHCGLPIEFH
                     RLQATPSATRCFDCQTSFERTHVQRTGHTL"
     gene            2857..3162
                     /locus_tag="PL152_01280"
     CDS             2857..3162
                     /locus_tag="PL152_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47933.1"
                     /translation="MDHGLKCWKCGASLADYTLPLRRLEECRACHAELHVCRMCEFYD
                     VSVAKHCRETIAEEVKDKVRANFCDYFRPAASAYRPGDVSAADRARADLEALFGGKK"
     gene            complement(3189..>3482)
                     /locus_tag="PL152_01285"
     CDS             complement(3189..>3482)
                     /locus_tag="PL152_01285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47934.1"
                     /translation="DRPCGQFAETRDVSFVEPGPGRVATVARPPAPATTRPRAEPRPP
                     DAKPDERAARCQRLLDQRDRLNARMREGYSATQSAQLWNRWRELAARIYAERC"
     assembly_gap    3483..3582
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3583..3865)
                     /locus_tag="PL152_01290"
     CDS             complement(<3583..3865)
                     /locus_tag="PL152_01290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47935.1"
                     /translation="MEILHRFVTACGAALFLACAVTVETEAATSGEADATGQPIEPAS
                     APEADIAAPSPTPSRRHRPIFVCHGARPVIFSDRPCGQFAETRDVSFVEP"
     gene            complement(3995..4279)
                     /locus_tag="PL152_01295"
     CDS             complement(3995..4279)
                     /locus_tag="PL152_01295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47936.1"
                     /translation="MSFDEAFGKAVDLGNQIADKDEKADLWDIADGLLAGAVQYWLYT
                     RQPCGDPLCEDCMPISTAEGRLELLRQLVREFSEESQYFHSPTDANVGRA"
     gene            complement(4422..4700)
                     /locus_tag="PL152_01300"
     CDS             complement(4422..4700)
                     /locus_tag="PL152_01300"
                     /inference="COORDINATES: protein motif:HMM:NF025715.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4404 family protein"
                     /protein_id="HQR47937.1"
                     /translation="MSELHDTLQGLHRELARADRLEPEDRALLETLLGDIRRLLETPK
                     VATAAPAAASAQQPGVALEGVAVKLEAGHPGVAAAIRAVLDALGKAGI"
     gene            4847..5488
                     /locus_tag="PL152_01305"
     CDS             4847..5488
                     /locus_tag="PL152_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47938.1"
                     /translation="MSGLRQALIAVTGVLACGLALAADAADKPRFELTPYVGYRMGGE
                     FDLDNPPPDTSSSADLDSSASWGVDLGIYRDRNSFYEILYSHQSAGVSTDDPSLDGKD
                     VSVQYLQAGGALLFPHEHYVPWLSLTIGATWFDAGGSYGSETKFSGSLGGGVRFPFGD
                     RFAATLGLRGYWTMVNSDTAFFCSGSGSVSCLFKTTGSAFFQGEALVGFTAVF"
     gene            complement(5502..9419)
                     /locus_tag="PL152_01310"
     CDS             complement(5502..9419)
                     /locus_tag="PL152_01310"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/FMN-binding oxidoreductase"
                     /protein_id="HQR47939.1"
                     /translation="MQGLDPPARLREIPYNYTSFSDREIVIRLLGDEGWRLLDELRSE
                     RRTGRSARMLYEVLGDIWVVQRNPYLEQDLLDNPRRRGQLIAALEHRLSDIDRRRDRS
                     DAGRDERVGRLLEAARAAVRDFARGFETVEALRRRALRLLRRHTRADNVCFDPYARVS
                     HATDATDWRVEIPFVVLAPDAEDEVPGLVRACVDLGLTIVPRGGGTGYTGGAVPLTPL
                     AAVINTEKLERVGSVEPADLPGVTGPVPTVFTEAGVVTRRVEEAAHKAGLVFAVDPTS
                     ADASCVGGNIAMNAGGKKAVLWGTALDNLAWWRMVDPDGNWLEVSRLNHNRGKIHDAA
                     TAQFELVWKDGSEAPARARVLRTERLEIEGRRFRKAGLGKDVTDKFLGGLPGVQKEGC
                     DGVITCARWVLHRMPAHVRTVCLEFFGQAREAIPAIVEIRDQLAARETQGVRLAGLEH
                     LDERYLKAVGYATKSKRGALPKMVLLGDIVGDDEAAVAHAASEVVRIANARSGEGFVA
                     AGAEARKTFWRDRARTAAIARHTNAFKVNEDVVIPLERLGEYTDAIERINIEFSLSNK
                     LALTEALEEYLAGSPRVGKTGDPDVDRLPHEDVLGERPQQALALVAATRARWSWLAGN
                     LDARVADALDTLGSLGLESRHAEFARRASESPGLRIFDLLQDRTLRVSWKTELRTELR
                     RLFAGGAFVPLVADLEDIHAKVLHGRVFVALHMHAGDGNVHTNIPVNSDDYAMLQAAN
                     RAVARIMRIARDLGGVISGEHGIGITKLEFLTDAETAEFRAYKGRVDPDGRFNKGKLL
                     PGADLRSAYTPSFNLLGHESLIMQQSDIASISDAIKDCLRCGKCKPVCSTHVPGANLL
                     YSPRNKILATSLLIEAFLYEEQTRRGVSLQHWDAFSDVADHCTVCHKCESPCPVDIDF
                     GDVSMAMRDLLRRVGKKRFNPGTSAAMFFLNATDPDTIRATRRLMVGWGFRAQRLGHR
                     ALGALGAAQKKRPPATTGGPAPVREQVVHFVNRKMPGGLPKRTARALLDIEDRNYVPI
                     LRDPARTTSESEAVFYFPGCGSERLFSQVGLATQAMLWEVGVQTVLPPGYVCCGYPQR
                     GTGQGDVAQKMITDNRVLLHRVANTLNYLDIRTVVVSCGTCYDQLQDYRFGEIFPGSR
                     ILDIHEYLLEKGVRVEGAQGVRYMYHDPCHSPIKTTDPLKVVNGLLDSGSRRIERNDR
                     CCGESGTFAVSRPDVATQVRFRKQREIERDVAGLREDGFAGETRILTSCPSCLQGLAR
                     YDDDTGTTADYIVVEIARKVLGEDWLPDYVRRANAGGIERVLV"
     gene            complement(9443..10870)
                     /gene="bamD"
                     /locus_tag="PL152_01315"
     CDS             complement(9443..10870)
                     /gene="bamD"
                     /locus_tag="PL152_01315"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HQR47940.1"
                     /translation="MALVLVRRPLPFILLLLGLVLAGCRSTGDVGPVDPPDKLYADAA
                     QDLRAANYKAAIQKLEALESHYPFTNAAKQGTLDLMFAYYKNGEGESAIDQADQFIRE
                     NPTHPRVDYAYYIKGLVYYESGAGWLEHVFRADVTKRPPNEARKSLQAFQTLLQQYPR
                     SPYAADARQRMVFLRNKLADYELAVARYYVKRQAYVAAVNRARAVIETYDGSPAALEA
                     LQIMQKGYRRLGIPELAQSAQVVYAANVNKPSVVESTAGLAGLAPSQGSDADWPEGKA
                     AADHAGQWEARVGVNYQNSSSTDFKGGTTVDVDSGLNFVGGVEYHFTDRISAGTSLSY
                     GKENYTAKVEGAGDQQGTVYPIKGDLTTTSLMVDGSYNFLTGRWSPFVTGGVGWSWVD
                     TNVATGPPEVGCWWSPWYGYVCTAWQDTKTESGLAYELGLGVRYDFNEYLAADGVYKM
                     KWIGFDNANGTPQYDGFQLNVGWKF"
     gene            10987..11946
                     /gene="rluD"
                     /locus_tag="PL152_01320"
     CDS             10987..11946
                     /gene="rluD"
                     /locus_tag="PL152_01320"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HQR47941.1"
                     /translation="MTASTVRISLEIPVGQAGQRLDQALAALLPDHSRSRLKSWIESG
                     EVLVDGSPRRPRDKVMGGESVAITATLAEETRAAPQDIPLVVVHEDRHVLVVDKPAGL
                     VVHPGAGNPDRTLQNALLARDPKLALLPRAGIVHRLDKDTSGLLVVARTLAAHTALVR
                     MLEERDIHREYEAVCRGVMTAGGTVDAPIARHPTDRVRMAVRQGGRESVTHYRVIRRF
                     RAHTHVRVQLETGRTHQIRVHLAHAGFPIVGDRVYGGRLAFPKGASDALREALRNFPR
                     QALHAARLEFAHPVSGKAVSCVAPLPADMQELLRVLEADAART"
     gene            11943..12692
                     /gene="pgeF"
                     /locus_tag="PL152_01325"
     CDS             11943..12692
                     /gene="pgeF"
                     /locus_tag="PL152_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HQR47942.1"
                     /translation="MSGRVEWVAPDWPAPAQVRVISTLRTGGASAGAYSSLNLAAHVG
                     DDPRCVRDNRLRLRLAAGLPAEPLWLEQVHGRDVVVHDQQAHAPRADAAIATTPHRVC
                     AVMTADCLPVVLADAGATRVGVAHAGWRGLAAGVVEATIAALGVAPAGLHAWLGPSIG
                     QSAFEVGGEVRQAFVAIANEAAACFAPNGRGRFQADLYGLARLALARAGVKSVHGGDW
                     CTATDRDRFFSFRRDGVTGRMATLAWLERRA"
     gene            12727..13506
                     /locus_tag="PL152_01330"
     CDS             12727..13506
                     /locus_tag="PL152_01330"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HQR47943.1"
                     /translation="MKTLVLIVLLTGLSGFLSALAASVFLLLDERRRVALLPHLVSFA
                     TGALLGAAFLGLVPHALESIGPGGAHRIGLGLLLGILTFFLLEKFVLWRHCHDDPCEM
                     HAPSHAARDAASARMILAGDSFHNVLDGVLVAAALMTDVRLGVVTALAVAAHEVPQEV
                     GDLAILLHGGYSRRRALALNLLTSLTSIAGGIVAYYALGTALSLLPYALAFAAASFIY
                     IAVADLIPGLHRRVDAWAGVEQVLFIVLGVALVYFAHQQMH"
     gene            13639..13953
                     /locus_tag="PL152_01335"
     CDS             13639..13953
                     /locus_tag="PL152_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47944.1"
                     /translation="MLPIRQFLRRVGRHALVSSALIAFSLGIGTLGYRAGARLGWIDA
                     FMNASMILTGMGPIAPMPTPGAKLFASFYALYSGIVFLAVSGILLAPFLHRMLHRFHL
                     DG"
     gene            14231..16849
                     /gene="clpB"
                     /locus_tag="PL152_01340"
     CDS             14231..16849
                     /gene="clpB"
                     /locus_tag="PL152_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HQR47945.1"
                     /translation="MRQDKLTSKFQMALADAQSLAVGRDHQFIEPVHLMMALLDQEGG
                     TVRPLLDKSGANVSRLRSQLGQALDGLARVEGTGGEVHVSNDLGKLLNVTDKLAQKRG
                     DQYVSSELFVLAALEDKGQLGRVLRDCGVVKGALEKAIDEVRGGEKVTDANAEEQRQA
                     LEKYTIDLTARAEQGKLDPVIGRDDEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIVNGEVPEGLRDKRILALDMGALIAGAKFRGEFEERLKGVLNDLAKQEGRVILF
                     VDELHTMVGAGKAEGAMDAGNMLKPALARGELHCVGATTLDEYRKYVEKDAALERRFQ
                     KVLVDEPSVEDTIAILRGLKERYAVHHKVEITDPAIVAAATLSHRYITDRQLPDKAID
                     LIDEAASRIRMEIDSKPEELDRLDRRIIQLKMEREALKKEADEASKKRLQALDEQLGV
                     LGREYADLEEVWKAEKAAVQGAASFKEELERARIDFEAARRAGDLARMSELQYGKIPE
                     LEKRIKAAQDAETKAPTLVRNKVTDEEIAEVVSKWTGIPVSKMLEGEREKLLRMEEAI
                     SRRVVGQDEAVKAVSDAIRRSRAGLSDPNRPNGSFLFLGPTGVGKTELSKALAEFLFD
                     TEEAMVRIDMSEFMEKHSVARMIGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNVLLQVLDDGRLTDGQGRTVDFRNTVIIMTSNLGSQVIQELAGEANYAKMKAA
                     VMEIVGSHFRPEFINRVDEVVVFHPLGHEHIRSIVDIQVGYLRKRLADRDIELALDDA
                     ARDLLGEAGFDPVYGARPLKRAIQHQLENPLAQAILRGEFGPGAHVLVTARDGKLAFE
                     QRGAAEAPAAAKRKKA"
     gene            17035..17460
                     /locus_tag="PL152_01345"
     CDS             17035..17460
                     /locus_tag="PL152_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HQR47946.1"
                     /translation="MPIYEYQCDHCGHHLEALQKISDKPLRECPECGRHRLKRLMSAP
                     LFRLAGSGWYETDFKSDKEQKRNLLEKGDKEPAPEAKAEAKPEGKAEAKAPEKTVVPA
                     AKPVATPAGAARPRAAKSAARSSAKAPVRKKAPARARKR"
     gene            17444..18175
                     /locus_tag="PL152_01350"
     CDS             17444..18175
                     /locus_tag="PL152_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQR47947.1"
                     /translation="MPASADPQGPTPEASPPAASGATGEHHPVRSFRNYLIGGLLVWI
                     PIMVTVWVIRFLSRILDQSLVLLPRAWRPETIFGTYVPGVGIVLSLLLLFLTGLVVKN
                     LFGGRIVAGLESLVRRIPVVGPIYGGAKSFSETVLTDKGRSFKQVVMVEFPRKGIYSI
                     GFITADDLEEVQAKTAQDVTCVFVPTTPNPTTGFIVLVPKEEVVALDMTVDEAIRMLL
                     TLGVVVPRWKGRGADARLAPQQTDP"
     gene            18242..20014
                     /gene="aspS"
                     /locus_tag="PL152_01355"
     CDS             18242..20014
                     /gene="aspS"
                     /locus_tag="PL152_01355"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HQR47948.1"
                     /translation="MRTHYCGEVNESLVGSTVSVAGWVHRRRDHGGVIFVDLRDREGL
                     LQVVFDPDAADVFAEAERVRGEWVLKVTGTVRPRPAGTANPHLASGQVELLARGLEVL
                     NRSEPIPFQLDEDVKEETRLKYRYLDLRRDEMQKRLRLRHAVTRAMRSYLDGHGFIDV
                     ETPMLTKATPEGARDYLVPSRTQPGKFFALPQSPQIFKQLLMMAGFDRYYQIVRCFRD
                     EDLRADRQPEFTQLDVETSFLARDEITGLMEGLVRELFDKVLGVELPDPFPRMTYAEA
                     MRRYASDKPDLRIPLELVDVADLVKDSEFKVFSAPAADKEGRVAALRVPGGGEKLSRK
                     QIDDYAAFVARYGAKGLAYVKVNDATKGRDGLQSPILKFLSDAAVTGLMQRTGAQTGD
                     LVFFGADKAKVVNDSMGALRLKVGQDLGLVEAGWRPLWVLDFPMFEWDPEAKRWAAMH
                     HPFTSPVNDDPAALKADPGGALARAYDMVLNGSEIGGGSVRIHRNEMQSTVFELLGIG
                     PEEAQKKFGFLLEALKFGAPPHGGIAFGLDRLAMLMAGADSIRDVIAFPKTQTATCPL
                     TDAPTEVSEQQLRELHIRVRLPEK"
     gene            20049..20699
                     /locus_tag="PL152_01360"
     CDS             20049..20699
                     /locus_tag="PL152_01360"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR47949.1"
                     /translation="MSPISPRDPVVLVHGLWMRGNEMALLRRRLQRDHGFDVHVFSYP
                     TLFGDTAQITADLADFAEGVAAGRLVHFVGHSLGGTFVLRALERYPARFDGNAVLLAA
                     PLNGCKAARGVLRWPMLRPIIGPHLLAEVSAPAEHHWQGPAALGAIAGTRRLGMGQFF
                     AGFHEDHDGTVAVSETIIPGLADHLVLPHSHMGMLTAGDVADQVAHFLRHAAFRRS"
     gene            complement(20696..22108)
                     /locus_tag="PL152_01365"
     CDS             complement(20696..22108)
                     /locus_tag="PL152_01365"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HQR47950.1"
                     /translation="MRRAVLLVACWSLTACLGTPYQRPKLDIPAQYRFTLSEAQRPAD
                     VQWWSSFGDPVLDELVLEALRNNEDLRIAAARVDEFHGAMVTTRAPIFPSVGYAASSG
                     QNKPSAQVVGKGNDVVANSSAAGFTAGWEIDVWGKIRNQTAAASADWRASDADRRAAV
                     LTLVSAVTSGYLTLLSLDDQLRTSEATLASRRKALDVFEKRYKGGVISKVELSQARYQ
                     YEVAAAAVPVLQEQIARQEDALSLLLGRNPGPIARGRVLLELGTPIVPPGLPSDILVQ
                     RPDVVSAEQSLIAANARVSAARALYFPSISLTGLLGVASLDLNKLFDGGSGTWSYAGQ
                     ATGPIFQGGSNVGVNEQADARREQLLANYEKTVQGAFADVDDALIATQKSTERTEAQR
                     LQVEALGDYARLARKRYEGGYSSYLEVLDAENSLFNAQLLYSQGQRDRFIAHVDLYKA
                     LGGGWVDAAAAKAPQPQYVP"
     gene            complement(22112..>22311)
                     /locus_tag="PL152_01370"
     CDS             complement(22112..>22311)
                     /locus_tag="PL152_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR47951.1"
                     /translation="ASRHSIGTGVIGGMVGATTLALFFVPLFYVVINELSERFFPPKA
                     PAAPAPATPGGDTATTGPGGH"
     assembly_gap    22312..22411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<22412..>25142)
                     /locus_tag="PL152_01375"
     CDS             complement(<22412..>25142)
                     /locus_tag="PL152_01375"
                     /inference="COORDINATES: protein motif:HMM:TIGR00915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR47952.1"
                     /translation="DTAQVQVQNRVNIALPQLPDVVQKTGVRVEKRSSSIMMLLAVYA
                     PDGGYDVNYVANYANLYVLDAVKRVPGANQATLFGAADNAMRIWLSPDRMAARGITST
                     DIQQAIAKQNQQFGAGSIGQSPTSRPVEVTVPVVTEGRFNEPAQFDDIILRTDPEGAA
                     IVRLGDVGRAEEGLQNYMMRPSLNGKPAVFIAVYQQPGSNALQVAQGVRKTLENLKKS
                     FPPGIDYEVSLDTTKFVTASIDEVVKTLLEAVVLVVLVVYVFLQNLRATLIPTIAVVV
                     SIVGTFVGMTMLGFSVNLLTLFGMVLSIGIVVDDAIVVIEAVEANMKGKGLSAREAAF
                     AAMHEVSGPVVAIVLVLAAVFVPTAFLGGTTGVLYKQFAMTVVISVILSGFVALTLTP
                     ALAALLLDGAHGHPPKVLQKFNEWFERLTESYTQGVRWVLKRAALALAVFAAFVVAIL
                     GLFKVVPGSFVPPEDQGFMIVAAILPDSASLDRSEAVGRRIAGIVLKHPAAKYASVLS
                     GYSAIDSQYKTNSATVFVSLKDFAERKDRSLSLDAMIAAIAPELAQIEDAYVIPINPP
                     PIPGLGMQGGFEMWVQDLTGGEPARLAQAVQQVLADAKQQPALAGVNSTFNPASRQLS
                     VKVDREKAETLGAPIADVYGALQSLFGSLYVSQYNKYGRVWQVVLQAEPEFRNSPEDL
                     RNIYVRGRSGEMVPLDAVTTARFTKGPDLIPRFNGFPAAKINGGPAPGYSSGQAIATM
                     EKLAATLPEGYGIAWSGQAYEEKQSGGASAMVFVFGLVMVFLILAAQYESWSLPGSVI
                     TAVPFGLLGALLAVWLRGMENDVYFQIGLVTLIGLAAKNAILIVEFAVLERQKGLSIV
                     ESAVEGAKARLRPIVMTSLAFTAGAIPLAIASGAGSASRHSIGTGVIGGMVGAT"
BASE COUNT         3837 a         8457 c         8688 g         3960 t
ORIGIN      
        1 ggagcgcgtc gagttcatcc acgagcagac gcattccgac gcgctggtgg aggaatacat
       61 cgaggggcgc gagatctacg tcggcgtcct cggcaacgac cggctcacga cgttcccgct
      121 ctgggaactc aatttcggca ccctgccgga ggtgatggcg ggcatcgcca cgcgcaaggt
      181 gaagtgggac ttgaagtacc agcgcaagca cggcatcggc accgggccgg cgcgcgacct
      241 gcccgaaggc atggagcacc gcatccgcca gctggtacgg cgcatctacc gggcgctgtg
      301 cctgagcggc tatgcgcgca tcgacttgag gctcaagccg gacgggagcg tgttcgtgct
      361 ggaggcgaac gccaatccga acctcgcgca gaccgaggat ttcgcgacct cggcgctggc
      421 ggccagcgtc ggctacgacg aactgctcga gcggatcatg aagctgggtg cggcctacga
      481 cgtcgcgtgg cggcagggct gagccgcagg ttgcaggtga cgaagggcag ccgataggct
      541 tgcgactgga acggcgccgg ggcggggttc gatgggcaag gtcgagcggt cattgcgagc
      601 ttcgctggcg gcgctgcgga tcgcgttgtc ctgtaccgtc gccctggcga cggccgccgt
      661 cgccggcggt gccgatgcgg gcggcgcgca ggctccggca gccggcgcgc ccgccgtgga
      721 cgagtcgacg caggcggtct tcgccgtcgc gaaccggccg atcttcacgc tgcgcgcctc
      781 gctgctcggg gcgacccctg ccgagcgcgt tgcagcctcg gaacaccggc tcgacagcat
      841 cgtgaagcat ggtgggccgc tcgtcgtctc cacgcgagac gtggacgacg gcgtcgcgct
      901 gctcgtggat ggcaagttcg tcgtgcgcgt gctgacgggt gacgtcgacc cggagtcggg
      961 tgagtccgag ctgagctacg cggccgccgc ggcgcacaac ctgcgcaagg cactcgcgga
     1021 gatccgcgag gcgaacgacg cgcgcgccat cggcagggcg gtggcatacg ccctcgcggc
     1081 gtccgtcctg ctcgcggtgt cctgctgggt gctgctccgg gcctaccggt ggctcgccgc
     1141 gagagtccac gcggccgcgc ggcgtcgact gggtcacctg ttgccggcgt gggccgacaa
     1201 ggtcatcggc gaggccgcac tgggcgggct gctcaccgtg cccttcaagc tgctggtcgt
     1261 ggtcgcgatc gcgttgctcg gctacgagtg ggccgggtac gtgctgcagc ggttcccata
     1321 cacccggccg tggggcgagc agctcagcga caacctgttc gaggccgcgg ggcggctcct
     1381 tcactcggta ctgtcggcca tcccgggcct gctgttcgtg gtgttcatct tcgtcgccgc
     1441 gcgaatcctg gtgcgcgcgg tgcgcgcatt cttcagtggc gtcgagcaag ggcagatcca
     1501 cgtcgagctg gtggagaaga ccacggcccg cccgaccggc aggctggtga cgaccgcgat
     1561 ctggctgttc gcgctcgtcg ccgcgtatcc ctacattccc ggtagtggca gcgaggcgtt
     1621 caggggcatc ggtgtgttcg tgggcctcat ggtgtcgatc ggggcgtcgg gtgtcgtcaa
     1681 ccaggccgtg agcggcctca tgctcatgta cacgaaagcc ctgcgccccg gcgaattcgt
     1741 caaggtcggc gacgtggagg gaacggtcac ttcggtcggg ttcgtcgcga cgcgcatcga
     1801 gaccctgcgg cacgaggagg tcaacgtgcc gaattccgtc atcgccacga acgtcatgca
     1861 caactattcg cggctgtcct cggagcgcgg cttgtggctc ccggcgacgg tcacgatcgg
     1921 gtacgacgcg ccgtggcggc aggtgcatgc gatgctgacg atcgcggcgg aacgcacgga
     1981 aggcgtggcg cacgacccgg cgccgcgtgt ccaccagacg gcgctgcagg acttctacgt
     2041 cgagtacacg ctgctggtca acgtcgcggt gccccgccac aagcgggaag tgctcacccg
     2101 gctgtacgcg aacatccagg acgtcttcaa cgagcacggt gtgcagatca tgtcgccgaa
     2161 ctacgaggac gacccggagt cgcccaagat cgtgccgaag gaaaaatggt acgagccgcc
     2221 ggcggcgcgc cccgaatagg cgcgccccgg tcagagcgtg tggccggtcc gctggacgtg
     2281 cgtgcgctcg aagctcgtct ggcagtcgaa gcagcgggtc gccgacggcg tggcctgcag
     2341 ccggtggaac tcgatcggca ggccgcagtg ctcgcaccgg ccgtagcttc cttcctgcac
     2401 gctgagcagg gcggcttcga cggccctgag ttcctcgagg tcgcggtcga tctccgcgag
     2461 gttcacgtcc acgatcaggt cggcgaacga ttcgtcctcg aggtcgcgca cctcgttggc
     2521 gacctgcgcg tactgctcgg aatcgctccg cagcagcgtc tggcggatct cctcgcgcag
     2581 caccttggcg cgctcgagca tcgtcgcttt cagttcttcc atctgtcgtg ggtcgaggtc
     2641 gcctcgcatc cgtgtctccc tgcgtccgat ccgggccggc cggaactgct tgcgcgagcg
     2701 aagcattgct cgcgcggccg tccgggccat atagggacat ggcgcaggtc gtatctcgga
     2761 aaattgccag cgcgggatcg gtcgtcccgc gagccgcgtt gacagggacc gggtggcggc
     2821 gtacctagga cgaccacggt cgcacgggag cgcgccttgg atcacggatt gaagtgctgg
     2881 aaatgcgggg cttccctcgc cgactacacc ttgccgctgc gccgcctcga ggagtgccgt
     2941 gcctgccacg ccgagctgca cgtctgccga atgtgcgagt tctacgacgt gtcggtcgcg
     3001 aagcactgcc gcgagaccat cgcggaggaa gtcaaggaca aggtccgcgc gaacttctgc
     3061 gattacttcc ggcctgctgc ctcagcctat cgtcccggcg acgtttcggc cgcggatcgg
     3121 gctcgcgccg acctcgaggc gctgttcggc ggcaagaagt agcggccggt cgcggccggg
     3181 cccgcgggtc agcagcgctc cgcatagatc cgggccgcga gttcgcgcca tcgattccac
     3241 agctgcgcag actgcgtcgc ggagtagcct tcgcgcatgc gggcattgag acggtcgcgc
     3301 tggtcgagca ggcgctggca gcgagcggcg cgctcgtcgg gcttcgcatc cggcggtcgc
     3361 ggttcggcgc gcggacgcgt ggttgcgggg gcgggcggtc gcgcaacggt cgcgacgcgg
     3421 ccggggcctg gctcgacgaa gctcacatcc cgcgtctccg cgaattgtcc gcaaggccgg
     3481 tcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnctggctcg acgaagctca
     3601 catcccgcgt ctccgcgaat tgtccgcaag gccggtcaga gaagatgacc ggcctggcgc
     3661 cgtggcacac gaagatgggc cggtggcgcc tggaaggcgt cggcgacggg gcagcgatat
     3721 ccgcctcggg agcggatgcg ggttcgatgg gctggcctgt agcgtcggcc tcaccgctgg
     3781 tcgccgcctc cgtctcgacg gtgactgcgc aggcaaggaa cagtgccgcg ccgcacgccg
     3841 ttacgaatcg atgcaggatt tccatgctcc gccctccatg gccgcgacga gcagcctgca
     3901 ccgcggagcg aggcggagcg aggcggatta acgccgcgat cgcccagaaa tgcggggcct
     3961 gcgactggcg cggtcgtggg cggtcggtgg tgagtcaggc ccgaccgacg ttcgcgtccg
     4021 tcggcgagtg gaagtactgg ctctcttccg agaattcgcg cacgagctgg cggagcagct
     4081 caaggcgtcc ctcggcggtg ctgatcggca tgcagtcctc gcagagaggg tcgccgcagg
     4141 gctgccgcgt gtacagccag tactggacgg cgccggcgag caggccgtca gcgatgtccc
     4201 agaggtccgc cttctcgtcc ttgtcggcga tctggttgcc gaggtcgacg gccttgccga
     4261 aggcctcgtc gaacgacatt gagtcttcct gcatgtgagt cgtgccgtag gtttcgcgcg
     4321 cgggtcgacg cggatgggcc ggttggaatg atagcagagc cagacgcgag gccatctgcg
     4381 ggcctggacg gcccgcacgg ccgacgggct gccgcctccg cctagattcc ggccttgccg
     4441 agcgcatcga gcacggcgcg gatcgccgcc gcaacgcccg ggtgcccggc ctcgagcttc
     4501 actgcgacac cttcgagcgc aacaccaggc tgctgtgccg aagccgccgc tggcgcggcc
     4561 gtcgcaacct tcggcgtttc gagcaaccgg cgtatgtcgc cgaggagtgt ctcgagcagg
     4621 gcgcggtcct ccggctcgag gcggtcggcc cttgccagtt cccggtgcag tccctgcagg
     4681 gtgtcgtgca gttcgctcat ggctcgtcca gtcgtctgtg aacgggatcc agtttagcag
     4741 cggcgcgggc cggacggcgc gggtgccgtc cgccggggcc cgcccgctcg cgtacaatcc
     4801 ggcgtccgca gcgccgcgcg ctgccgggtc gtccaaagga gcaggcatga gcgggctgag
     4861 gcaggcgctg atcgcggtga ctggcgtgct ggcctgcggt ctggccctgg cggccgacgc
     4921 cgccgacaag ccgcgcttcg aactgacccc gtacgtgggt tatcggatgg gcggcgagtt
     4981 cgacctcgac aatccgccgc ccgatacctc cagttccgcg gacctcgaca gcagcgcctc
     5041 ctggggcgtg gacctcggga tctaccgcga ccgcaattcc ttctacgaga tcctctacag
     5101 ccatcagtcg gcaggcgtca gtaccgacga cccgtcgctc gatggcaaag acgtctcggt
     5161 ccagtacctg caggcgggcg gggccttgtt gttcccgcac gagcactacg tgccctggct
     5221 gtcgctcacg atcggcgcga cgtggttcga tgcgggcggt tcctacggct cggagacgaa
     5281 gttttccggc agcctcggcg gcggtgtccg ctttcctttc ggcgaccggt tcgcggcgac
     5341 cctgggactg cgcggctact ggaccatggt caattccgac actgcgttct tctgctccgg
     5401 cagcggcagc gtcagctgcc tgttcaagac gacgggtagc gccttcttcc agggcgaggc
     5461 gttggtcgga ttcaccgctg tgttctgatc ggcggggccg ctcacaccag cacgcgctcg
     5521 atgccaccgg cattggcgcg gcgcacgtag tccgggagcc agtcctcgcc gagcaccttg
     5581 cgcgcgatct cgacgacgat gtagtcggcc gtcgtgccgg tgtcgtcgtc gtagcgcgcg
     5641 agtccctgca ggcaggacgg gcacgaagtc aggatgcgcg tctcgccagc aaagccgtcc
     5701 tcgcgcaggc ccgcgacgtc gcgctcgatc tcgcgctgct tgcggaaccg cacctgcgtc
     5761 gccacgtcgg gccgcgacac cgcgaacgtg ccggactctc cgcagcagcg atcattgcgc
     5821 tcgatccggc gagaccccga gtcgagcagg ccgttcacca ccttgagcgg gtcggtggtc
     5881 ttgatcggcg agtggcaagg gtcgtggtac atgtagcgca cgccctgggc gccctcgact
     5941 cgcacgcctt tctcgagcag gtactcgtgg atgtcgagga tgcggctgcc cgggaagatc
     6001 tcgccgaacc ggtagtcctg cagctggtcg tagcaggtgc cgcagctcac gacgaccgtg
     6061 cggatgtcca ggtaattgag cgtgttcgcg acccggtgca gcaggacgcg gttgtccgtg
     6121 atcatcttct gcgcgacgtc gccttgcccg gttccccgct gcgggtatcc gcagcagacg
     6181 tagccgggcg gcagcaccgt ctgcacgccg acctcccaca gcatggcctg cgtggccagc
     6241 ccgacctgcg agaacaggcg ttccgagccg cagcccggga aatagaacac cgcttccgac
     6301 tcgctcgtgg tgcgtgccgg atcccggagg atcggcacgt agttgcggtc ctcgatgtcg
     6361 agcagggcgc gtgccgtgcg cttcggcagg ccgccgggca tcttgcggtt cacgaagtgg
     6421 acgacctgtt cccgaaccgg cgccggcccg cccgtcgtcg ccggcgggcg cttcttctgc
     6481 gcggcgccga gcgcaccgag cgcgcggtga ccgagccgct gcgcgcgaaa gccccagccg
     6541 accatcagcc tgcgcgtggc gcggatcgtg tccgggtccg tggcgttcag gaagaacatc
     6601 gcggccgacg tgccggggtt gaagcgcttc ttgccgacgc gccgcagcag gtcgcgcatc
     6661 gccatggaca cgtcgccgaa gtcgatgtcg acggggcagg gcgactcgca cttgtggcag
     6721 acggtgcagt ggtcggccac gtccgagaac gcgtcccagt gctgcagcga gacgccgcgc
     6781 cgcgtctgct cctcgtagag gaaggcctcg atcagcagcg aggtcgccag gatcttgttg
     6841 cgcggcgaat agagcaggtt ggcgccgggc acgtgcgtgg agcacaccgg cttgcacttg
     6901 ccgcagcgca ggcagtcctt gatcgcgtcc gaaatggagg cgatgtcgga ctgctgcatg
     6961 atgagcgact cgtgcccgag caggttgaag ctcggcgtgt aggcgctgcg caggtcggcg
     7021 ccgggcagca gcttgccctt gttgaagcga ccgtccgggt cgacgcgccc cttgtacgcg
     7081 cggaactctg cggtctccgc gtcggtgagg aactcgagct tggtgatgcc gatgccgtgc
     7141 tcgccagaga tgacgccgcc gaggtcgcgg gcgatgcgca tgatgcgcgc caccgcacgg
     7201 ttcgctgcct gcagcatcgc atagtcgtcc gagttcaccg ggatgttggt gtgcacattg
     7261 ccgtcgcccg cgtgcatgtg cagcgcgacg aacacgcggc cgtgcaggac cttcgcgtgg
     7321 atgtcctcga ggtccgcgac gagcggcacg aaggcgccgc ccgcgaacag ccgtcgcagt
     7381 tcggtccgca gctccgtctt ccacgacaca cgcagggtgc ggtcctggag caggtcgaag
     7441 atccgcagtc cgggtgattc cgacgctcgt cgcgcgaact cggcgtgccg cgactcgagg
     7501 ccgagtgagc caagcgtgtc gagcgcgtcc gcgacgcgcg cgtcgaggtt gccggccagc
     7561 catgaccagc gagcccgggt cgccgcgacc agtgcgagcg cctgctgtgg ccgctcgccg
     7621 agcacgtcct cgtgcggcag ccggtccacg tcggggtcgc cggtcttgcc gacgcgcggc
     7681 gagccggcga ggtactcctc gagcgcctcg gtgagcgcca gcttgttcga cagagagaac
     7741 tcgatgttga tgcgctcgat ggcgtcggtg tactcgccga gtcgctcgag cgggatcacc
     7801 acgtcctcgt tcaccttgaa ggcgttggta tggcgcgcga tggccgccgt gcgcgcgcgg
     7861 tcacgccaga acgtcttgcg ggcctccgct ccggcggcga cgaagccctc cccggagcgg
     7921 gcgttcgcga tgcgcacgac ctcggatgcc gcgtgcgcca ccgcagcctc gtcgtcgccg
     7981 acgatgtcgc cgagcagcac catcttcggc agggcgccgc gcttcgactt ggtggcgtag
     8041 ccgaccgcct tgagatagcg ctcgtcgagg tgctccagac cagccaggcg gacgccctgg
     8101 gtttcgcgcg ccgcgagctg gtcgcggatc tcgacgatgg ccgggattgc ctcgcgcgcc
     8161 tgcccgaaga actccaggca gaccgtgcgg acgtgcgccg gcatgcggtg cagcacccat
     8221 cgcgcgcacg tgatgacgcc gtcgcagcct tccttctgca cgccgggcag gccgccgagg
     8281 aacttgtcgg tgacgtcctt gccgagcccc gccttgcgga agcgccgtcc ctcgatctcg
     8341 agccgctcgg tgcgcaggac acgggcacgc gccggtgcct ccgatccgtc cttccacacg
     8401 agttcgaact gcgctgtcgc ggcgtcgtgg atcttgcccc tgttgtgatt gaggcggctc
     8461 acctcgagcc agttgccgtc ggggtcgacc atgcgccacc acgcgaggtt gtcgagcgcg
     8521 gtgccccaca gcacggcctt cttgccgccg gcattcatgg cgatgttgcc gccgacgcag
     8581 gacgcatcgg cggacgtcgg gtccaccgcg aatacgaggc ccgccttgtg ggccgcctcc
     8641 tcgacgcggc gcgtgacgac acccgcttcc gtgaacacgg tcggcaccgg cccggtgact
     8701 ccggggaggt cggccggttc gacgctgccg acgcgctcga gcttctcggt gttgatgacg
     8761 gccgccagcg gcgtcaacgg cacggcgcca ccggtgtagc ccgtgccgcc gccgcggggg
     8821 acgatcgtga ggccgagatc cacgcaggcg cgcaccaggc cggggacctc gtcctccgcg
     8881 tcgggcgcca gcaccacgaa gggaatctcg acgcgccagt ccgtcgcgtc ggtggcgtgc
     8941 gagacgcgtg cgtacgggtc gaagcagacg ttgtcggcgc gcgtgtgccg gcgcagcagt
     9001 cgcagcgcgc ggcgccgcag ggcctccacc gtttcgaatc cgcgggcgaa atccctgacc
     9061 gcggcccgcg ctgcctcgag cagccggccc acgcgttcgt cgcgcccggc gtcggagcgg
     9121 tcgcggcggc ggtcgatgtc ggacaggcga tgttcgagcg cggcgatcag ctgcccgcgc
     9181 cgccggggat tgtcgagcag gtcctgctcg aggtacgggt tgcgctgcac gacccagatg
     9241 tcgcccagca cctcgtagag catgcgcgcc gagcggcccg tgcgtcgctc ggaacgcaac
     9301 tcgtcgagca gccgccagcc ctcgtcgccg agcaggcgga tcacgatctc gcgatcggag
     9361 aacgacgtgt agttgtaggg gatctcgcgc agccgcgccg gcgggtcgag tccctgcatc
     9421 gtgtgtgcgc cggccgtcgc ggtcagaact tccagccgac gttgagctgg aatccgtcgt
     9481 actggggtgt gccgttggcg ttgtcgaagc cgatccactt catcttgtac acgccgtcgg
     9541 ccgcgaggta ctcgttgaaa tcgtagcgca cgcccaggcc gagttcgtac gcgaggccgc
     9601 tctcggtctt ggtgtcctgc cacgcggtgc agacgtatcc gtaccacggg ctccaccagc
     9661 aaccgacctc gggcgggccg gtggccacgt tggtgtcgac ccagctccag ccgacgccgc
     9721 cggtgacgaa tggcgaccag cggcccgtga ggaaattgta cgagccgtcc accatcagcg
     9781 acgtcgtcgt gaggtcgccc ttgatcggat acacggtgcc ctgctggtcg cccgcgccct
     9841 ccaccttggc ggtgtagttc tccttgccgt aactcaggct ggtgccggcg gatatgcggt
     9901 cggtgaaatg gtattccacg ccgccgacga agttgaggcc gctgtcgacg tcgaccgtcg
     9961 tgccaccctt gaagtcagtg ctggacgaat tctggtagtt caccccgacc cgcgcttccc
    10021 actggccggc gtgatccgcc gcggccttgc cctccggcca gtccgcgtcg ctaccctgcg
    10081 acggagccaa gcccgcgaga ccggctgtgg actcgacgac gctcggcttg ttcacgttgg
    10141 ccgcgtacac gacctgcgcg gactgggcca gttccgggat cccgagcctg cggtatccct
    10201 tctgcatgat ctgcagggcc tcgagcgccg ctggcgaacc gtcgtaggtc tcgatcacgg
    10261 ctcgcgcgcg attcaccgcg gcgacgtagg cctggcgctt cacgtagtaa cgtgcgaccg
    10321 ccagttcata gtcggcgagc ttgttcctca ggaacaccat gcgctgccgg gcatccgcgg
    10381 catacgggct cctcggatac tgctgcagca gcgtctggaa cgcctgcagg gacttgcggg
    10441 cctcgttggg cgggcgcttc gtcacgtcgg cgcggaatac gtgctcgagc cagccggctc
    10501 ccgactcgta gtacacgagg cccttgatgt agtaggcgta gtcgacccgc gggtgcgtcg
    10561 ggttctcgcg tatgaactgg tcggcctggt cgatcgccga ctcgccttcg ccattcttgt
    10621 aataggcgaa catcaggtcg agcgtgccct gcttcgccgc gttggtgaac gggtagtgcg
    10681 actcgagcgc ctcgagtttc tggatggccg ccttgtagtt ggccgcgcgc aggtcctgcg
    10741 cagcgtccgc atagagcttg tcgggtggat ccaccgggcc gacgtcgccc gtgctccggc
    10801 aacccgccag aaccaagccc aggagcaaga ggatgaacgg cagcggtcgg cgaacgagga
    10861 ctagtgccat gggcggtcgt gaaaaggggc ggcgcggctc gcggggggcg gccggaagta
    10921 tatagaaagc tggcgcgccc cgtagttccg gtacacaagg gccgggtcat cggccagaat
    10981 cgcgcgatga cggcctcgac agtgcggatt tcgctcgaga taccggtggg ccaggccggt
    11041 cagcgcctcg accaggcgtt ggccgcactg cttccggacc actcgcgcag ccgcctgaag
    11101 tcgtggatcg agtccggcga ggtgctcgtc gacgggtcgc cgcgcaggcc gcgcgacaag
    11161 gtcatgggtg gcgaaagcgt cgcgatcacc gccacgctgg ccgaagagac gcgggccgcg
    11221 ccgcaggaca tcccgctcgt cgtcgtgcac gaggaccggc acgtgctggt ggtggacaag
    11281 cccgcgggac tcgtcgtgca ccccggcgcc gggaaccccg atcgcacgct gcagaatgcg
    11341 ctgctcgccc gcgacccgaa gctcgcgctc ctgccgcgcg ccggcatcgt gcatcgcctc
    11401 gacaaggaca cgagcggcct gctcgtcgtg gcgcgcacgc tcgccgccca caccgcgctc
    11461 gtcaggatgc tcgaggaacg cgacattcat cgggaatacg aggccgtctg ccggggcgtg
    11521 atgacggccg ggggcacggt ggatgcgccg atcgcgcgcc atccgacgga ccgcgtgcgg
    11581 atggccgtgc ggcagggtgg ccgtgagtcc gtgacccact atcgcgtgat ccgccggttc
    11641 cgcgctcaca cgcacgtgcg cgtgcaactg gagaccggcc gcacccacca gatccgcgtg
    11701 cacctcgccc acgcggggtt cccgatcgtc ggcgaccgcg tctacggcgg gcgcctggcg
    11761 ttccccaagg gcgcgagcga tgcgctgcgc gaggcgctgc ggaatttccc gcggcaggcg
    11821 ttgcatgcgg ccaggctcga attcgcgcac ccggtgagcg gcaaggcagt ttcgtgcgtc
    11881 gcgccgctgc cggccgacat gcaggaactc ctgcgcgtgc tcgaagcgga cgcggcgcgc
    11941 acgtgagcgg gagggtggag tgggtcgcgc cggactggcc cgcgccggcg caggtgcgcg
    12001 tgatctcgac cctgcgcacc ggaggcgcga gcgcaggcgc gtattcttcg ctcaacctgg
    12061 cggcccacgt cggggacgat ccgcgctgcg tgcgggacaa ccgcctgcgt ctacgccttg
    12121 cggcagggct gcccgccgaa ccactgtggc tcgagcaggt gcacggccgg gacgtcgtcg
    12181 tgcacgacca gcaggcgcat gcgccccgcg ccgatgccgc gattgccacg accccgcacc
    12241 gggtgtgcgc ggtcatgacg gcggattgcc tgcccgtcgt cctggccgac gccggtgcaa
    12301 cgcgggtcgg cgtcgcgcat gccgggtggc gcggactggc ggccggcgtc gtcgaggcga
    12361 cgatcgcggc gctcggggtc gccccggcag gcctgcacgc atggctggga ccgtcgatcg
    12421 ggcagtcggc cttcgaggtc gggggcgaag tgcgacaggc attcgtcgcg atcgcgaacg
    12481 aagcggccgc ctgtttcgcc ccgaacgggc gcgggcgttt ccaggccgac ctgtacggcc
    12541 tcgcgcgcct ggcactggcc cgcgccggag tgaagtcggt gcacggaggc gattggtgca
    12601 ccgccaccga ccgggatcgc ttcttctcgt tcaggcgcga cggagtgacc gggcgcatgg
    12661 cgaccctggc gtggctcgag cgacgcgcgt gataggctca acggaacgag gtccgcgcaa
    12721 cacgccgtga agacgctagt cctgatcgtc ctgctgaccg gcttgagcgg cttcctgagt
    12781 gcgctggccg cctcggtgtt cctgctgctc gatgaacgac ggcgcgtcgc actcctgccg
    12841 cacctcgtga gcttcgccac cggcgcgctg ctcggcgccg ctttcctcgg cctggtgccg
    12901 cacgcgctcg agtcgatcgg cccgggaggc gcacaccgca tcgggctcgg cctcctgctc
    12961 gggatcctga ccttcttcct gctcgagaag ttcgtgctgt ggcggcactg tcacgacgat
    13021 ccctgcgaaa tgcatgcacc gagccacgcg gcgcgggacg ccgcgtcggc gcgcatgatc
    13081 ctggcgggcg acagcttcca caacgtgctg gacggcgtgc tggtcgccgc ggcgctcatg
    13141 accgacgtac ggctcggcgt ggtcacggcg ctggcggtcg cggcgcacga ggtgccgcag
    13201 gaggtcgggg acctagccat cctgctgcac ggcgggtact cgcgccggcg cgcgctggca
    13261 ctcaacctgc tgacgagcct cacgtccatt gccggcggca tcgtggctta ctacgcgctg
    13321 gggaccgcgc tcagcctgtt gccctacgcg ctggccttcg cggcggcgag cttcatctac
    13381 atcgcggtcg cggacctcat tcccggcctg catcgccgcg tggacgcctg ggccggcgtc
    13441 gagcaggtgc tgttcatcgt gctcggcgtg gccctcgtgt acttcgcgca ccagcagatg
    13501 cactgacggc ggcgtctgaa cgaagcgccg ggcctccagc tcgggtcggc acggattcct
    13561 gcgacacgcc ggtgcaggct aggctatcgg gcacacttcc cgggagatca ggctgtgtac
    13621 gagcatcatc gggagccgat gcttcccatc cggcaattcc tgaggcgcgt cggccgccac
    13681 gcgctcgtca gttctgcctt gatcgcgttc tcgctgggaa tcgggacgct cggctatcgc
    13741 gccggtgccc ggctcggctg gatcgacgcc ttcatgaacg cgtcgatgat cctcaccgga
    13801 atggggccga tcgcgccgat gccgacgccc ggcgcgaagc tgttcgcatc cttctacgcg
    13861 ctgtacagcg ggatcgtgtt cctggccgtt tccgggatcc tgcttgctcc attcctgcac
    13921 cggatgctgc accggttcca cctcgacggc tgagcctgct cggcatggcc ggcgccgggc
    13981 ggcgcgggga gatgccactt gcggggagaa aaggcgtctt ctggcgtcga ttgtgcaaaa
    14041 tactgcggct aacgtcgtcg aaaaagctcc tatccgcctg gcgcggacgc tgattcgcag
    14101 cttcgccgca cggccccggg caggggtttg accgccccga ccccggcaac aacgctgccg
    14161 caccctttat ctcgtcccgt gcgccccaag actagggcac accgcagccc ctcatgggag
    14221 cccccgactc atgcgacagg acaagttgac cagcaagttc cagatggccc tcgccgatgc
    14281 ccagtcgctg gcggtcggtc gcgaccacca gttcatcgag cccgtgcacc tgatgatggc
    14341 gttgctcgac caggagggcg ggaccgtccg gccgctgctc gacaagtccg gcgccaacgt
    14401 gagtcgcctg cgctcgcagc tcgggcaggc gctcgacggc ctcgcgagag tggaagggac
    14461 gggcggcgag gtccacgtat ccaacgacct cggcaagctg ctgaacgtca ccgacaagct
    14521 cgcgcagaag cgcggggacc agtacgtctc gagcgagctg ttcgtgctcg cggccctcga
    14581 ggacaaaggc cagctcggtc gcgtgctgcg cgactgcggc gtggtcaagg gagcgttgga
    14641 aaaggccatt gacgaagtga ggggcggcga aaaggtcacc gatgcgaatg ccgaggagca
    14701 gcggcaggcg ctcgagaagt acacgatcga cctgaccgcg cgcgccgagc agggcaagct
    14761 cgacccggtc atcggccgcg acgacgagat ccgccgcacc atccaggtgc tgcagcggcg
    14821 caccaagaac aacccggtgc tgatcggcga gcccggcgtc ggcaagaccg ccatcgtcga
    14881 aggactcgcg cagcgcatcg tgaacggcga agtcccggag ggattgcgcg acaagcgcat
    14941 cctcgcgctc gacatgggcg cgctcatcgc cggtgccaag ttccgcggcg aattcgagga
    15001 gcgcctgaag ggcgtgctca atgacctcgc gaagcaggag ggtcgcgtca tcctgttcgt
    15061 cgacgagctg cacacgatgg tcggcgcggg caaggccgag ggcgcgatgg acgcgggcaa
    15121 catgctgaag cccgcgctgg cgcgcggcga gctgcactgc gtcggcgcca cgacgctcga
    15181 cgagtatcgc aagtacgtcg agaaggacgc cgcgctcgag cggcgcttcc agaaggtgct
    15241 ggtcgacgag ccctcggtcg aggacacgat cgcgatcctg cgcggcctga aggagcgcta
    15301 tgccgtgcac cacaaggtcg agatcaccga tcccgccatc gttgcggcgg ccacgctctc
    15361 gcaccgctac atcaccgacc gccagctccc cgacaaggcg atcgacctga tcgacgaggc
    15421 ggcgtcgcgg atccgcatgg agatcgactc caagcccgag gaactcgacc ggctcgaccg
    15481 tcgcatcatc cagctcaaga tggagcgcga ggcgctcaag aaggaggccg acgaggcctc
    15541 gaagaagcgc ctgcaggcgc tcgacgagca gcttggcgtc ctcgggcgcg agtacgcgga
    15601 cctcgaggag gtctggaagg ccgaaaaggc tgcggtgcag ggtgccgcga gcttcaagga
    15661 ggaactcgag cgtgcgcgca tcgacttcga ggcggcgcgt cgcgccggcg acctcgcgcg
    15721 catgtcggag ctgcaatacg ggaagattcc cgagctcgag aagcgcatca aggccgcgca
    15781 ggacgccgag accaaggcgc cgacactggt gcgcaacaag gtgactgacg aggagatcgc
    15841 ggaagtcgtc tccaagtgga ccggcatccc ggtctcgaag atgctggaag gcgagcgcga
    15901 gaagctcctg cgcatggaag aagccatctc ccggcgcgtc gtcggccagg acgaggccgt
    15961 gaaggccgtt tccgacgcca tccggcgctc gcgcgccggg ctgtcggacc ccaatcgccc
    16021 gaatggttcg ttcctgttcc tcgggccgac cggcgtgggc aagaccgagc tgtccaaggc
    16081 gctcgccgag ttcctcttcg acaccgagga ggccatggtg cgcatcgaca tgtccgagtt
    16141 catggagaag cactccgtgg cgcggatgat cggcgcgccc ccgggctacg tcggttacga
    16201 ggaaggtggc tacctcacgg aagcggtgcg tcgccgcccg tactcggtga tcctgctcga
    16261 cgaggtcgag aaggcgcacc cggacgtgtt caacgtcctg ctgcaggtgc tcgacgacgg
    16321 ccgcctgacg gacgggcagg gccgcaccgt ggacttccgc aacaccgtca tcatcatgac
    16381 ctcgaacctc ggttcgcagg tcatccagga gctggcgggc gaggccaact acgcgaagat
    16441 gaaggccgcg gtgatggaga tcgtcggcag ccacttccgg ccggaattca tcaaccgcgt
    16501 cgacgaggtc gtggtgttcc acccgctcgg ccacgagcac atccgctcga tcgtggacat
    16561 ccaggtcggc tacctgcgca agcggctcgc ggaccgcgac atcgagctcg cgctcgacga
    16621 cgccgcgcgc gacctgctcg gcgaggctgg attcgacccg gtgtacggcg cgcgaccgct
    16681 caagcgcgcg atccagcacc agctcgagaa tccgctcgcg caggccatcc tgcgcggcga
    16741 gttcgggccc ggagcgcacg tcctggtcac ggcgcgcgac ggcaagctcg ccttcgagca
    16801 gcgcggcgct gcggaggcgc ccgccgccgc gaagcggaag aaagcctgac gggcgtgaca
    16861 caggcgatcc acgccgcgtg cctgcgcgag gacgccgtga acccgctccc tgggggctcc
    16921 gggcgccgcg tccatgcggc gcacggcctc gcgcaggcac acggcatgga tcgcccggtc
    16981 cgcggccacc gagctctcgt ataatcgccc gcccggagga gcatcgacgc gaccatgccc
    17041 atctacgagt accagtgcga ccactgcggc caccacctcg aggcgctgca gaagatttcc
    17101 gacaagcccc tgcgcgaatg cccggagtgc ggtcggcatc gcctgaagcg cctcatgtcg
    17161 gcgccgctgt tccgactcgc gggcagcggc tggtacgaga ccgatttcaa gtccgacaag
    17221 gagcagaaac gcaacctgct cgagaagggc gacaaggaac ccgcccccga ggcgaaggcg
    17281 gaagccaagc cggaagggaa ggccgaggcc aaggcgcctg agaagacggt cgttcccgcg
    17341 gccaagcccg tcgcgacgcc ggccggcgcc gcgcgaccgc gggcggcaaa gagcgcggcg
    17401 aggtcatcgg cgaaggcgcc ggtacgcaag aaggcccctg cgcgtgcccg caagcgctga
    17461 tccgcagggc ccgacgcccg aagcgtcgcc tcccgccgcc agcggtgcga caggggagca
    17521 tcacccggtt cgatcgttcc gcaattacct gatcggcgga ttgctggtct ggatccccat
    17581 catggtcacc gtctgggtga tccggttcct gtcgcgcatc ctcgaccagt cactggtgct
    17641 gctgccgcgc gcctggcgac ccgaaacgat cttcggtacg tatgttccgg gagtcggcat
    17701 cgtcctgtcg ctgctcctgt tgttcctcac gggactcgtg gtcaagaacc tgttcggcgg
    17761 acgcatcgtc gccgggctcg agtcgctggt gcggcgcatc ccggtcgtcg gcccgatcta
    17821 cggcggcgcc aagtcgttca gcgagacggt gctcaccgac aagggccgct cgttcaagca
    17881 ggtggtgatg gtcgagttcc cgcgcaaggg gatctacagc atcggcttca tcacggccga
    17941 cgacctcgag gaggtccagg ccaagaccgc ccaggacgtc acctgcgtgt tcgtcccgac
    18001 cacgccgaat ccgaccaccg ggttcatcgt cctcgtgccg aaggaggagg tggtcgcgct
    18061 cgacatgacc gtcgacgagg cgatcaggat gctgctcacg ctgggcgtgg tcgtgccgcg
    18121 ctggaagggg cggggtgcgg acgctcgtct tgcgccgcag cagaccgacc cgtaacatcc
    18181 cgtcccccct gagaaaaacc ggcctcgggg cgccggcaga ccggccccgc caaccatgtc
    18241 catgcgcacc cattactgcg gcgaggtcaa cgagagcctc gtcggaagca ccgtctccgt
    18301 cgccgggtgg gtccaccgcc ggcgtgacca cggcggagtg atcttcgtcg acctccgcga
    18361 tcgcgagggc ctgctgcagg tcgtcttcga tcccgacgcg gcggacgtct tcgcggaagc
    18421 ggagcgcgtg cgcggcgaat gggtgctcaa ggtgaccggc accgtgcggc cgcgtcctgc
    18481 cggcacggcc aatccgcacc tcgcatccgg ccaggtggaa ctcctcgcgc gaggcctcga
    18541 ggtgctgaac cgctccgagc cgatcccgtt ccagctcgac gaggacgtga aggaagagac
    18601 gcggctcaag taccgctacc tcgacctgcg tcgcgacgag atgcagaagc gcctgcgcct
    18661 gcgccacgcg gtgacgcgcg cgatgcgcag ctacctcgac ggccacggct tcatcgacgt
    18721 cgagacgccg atgctgacca aggcgacgcc cgagggcgcg cgcgattacc tcgtgccgag
    18781 ccgcacgcag ccgggcaagt tcttcgcgct gccgcagtcg ccgcagatct tcaagcagct
    18841 gctgatgatg gcgggcttcg accgctacta ccagatcgtg cgctgcttcc gcgacgagga
    18901 cctgcgcgcc gatcgccagc ccgagttcac ccagctcgac gtcgaaacct cgttcctcgc
    18961 gcgcgacgag atcaccggcc tgatggaagg gctggtgcgg gagctcttcg acaaggtgct
    19021 cggcgtcgag ctgccggatc ccttcccgcg catgacgtat gccgaggcga tgcgccgcta
    19081 cgcgtccgac aagccggacc tgcgcatccc gctggaactc gtggacgtcg ccgacctggt
    19141 gaaggacagc gaattcaagg tgttctcggc gccggccgcg gacaaggagg gtcgcgtcgc
    19201 ggcactgcgc gtgcccggtg gcggcgagaa gctctcgcgc aagcagatcg acgactacgc
    19261 cgcgttcgtc gcgcggtacg gcgcgaaggg cctcgcgtac gtcaaggtca acgacgcgac
    19321 gaagggacgc gacggcctgc agtcgccgat cctgaagttc ctgtcggacg ccgcggtgac
    19381 ggggctgatg cagcgcacgg gcgcgcaaac cggcgacctc gtgttcttcg gtgccgacaa
    19441 ggcgaaggtc gtgaacgatt cgatgggcgc gctcaggctc aaggttggcc aggacctcgg
    19501 gctggtcgag gcgggctggc gcccgctgtg ggtgctcgat ttcccgatgt tcgaatggga
    19561 cccggaggcg aagcgctggg ccgcgatgca ccatccgttc acctcgccgg tcaacgacga
    19621 cccggccgca ctcaaggccg atccgggcgg agcgctggcc cgcgcgtacg acatggtgct
    19681 gaacggctcg gagatcggcg gcgggtcggt ccgtatccac cgcaacgaga tgcagtccac
    19741 cgtgttcgag ctgctcggca tcgggccgga ggaagcgcag aagaagttcg gcttcctgct
    19801 cgaggcgctg aaattcggcg cgccgccgca cggcggcatc gcattcggcc tcgatcgcct
    19861 cgcgatgctg atggcagggg cggactcgat ccgtgacgtc atcgccttcc cgaagacgca
    19921 gaccgcgacc tgcccgctga ccgacgcgcc aaccgaggtg tccgagcagc agttgcgcga
    19981 gctgcacatc cgcgtgcgtc tgccggagaa ataggggaca ttctcctatt tcgaactaaa
    20041 acaggagaat gtccccaatt tctccgcgcg acccggtcgt gctcgtccat ggactgtgga
    20101 tgcgaggcaa cgaaatggcc ctgctgcggc ggcgcctgca gcgcgaccac ggattcgacg
    20161 tccacgtgtt ctcgtatccg acgctgttcg gtgacaccgc gcagatcacg gcggacctcg
    20221 ccgatttcgc agagggagtc gcagctggtc ggctcgtgca cttcgtcggc cacagcctcg
    20281 gcggcacgtt cgtgctgcgc gcgctcgagc gatatccggc gcggttcgac ggcaacgccg
    20341 tcctgctcgc ggccccgttg aacggctgca aggccgcgcg cggcgtgttg cgctggccga
    20401 tgctgcggcc gatcatcggg ccgcacctgc tcgccgaggt ctccgcgccg gccgagcacc
    20461 actggcaggg tccggcggcg ctcggtgcga tcgcgggcac gcgcaggctc ggcatgggcc
    20521 agttcttcgc ggggttccac gaggaccacg acggcacggt ggccgtcagc gagacgatca
    20581 tccccggtct cgccgatcac ctcgtgctgc cccacagtca catgggcatg ctcacggccg
    20641 gcgacgtcgc cgaccaggtg gcgcatttcc tccggcacgc tgcgtttcga cgctcctagg
    20701 gcacgtattg cggctgcggc gccttcgcgg cagcggcatc cacccagcca ccgccaagcg
    20761 ccttgtacag atcgacgtgc gcgatgaatc ggtcgcgctg cccttggctg tagagcagct
    20821 gcgcgttgaa gagcgaattt tccgcatcga gcacttcgag gtaactcgaa tagccgccct
    20881 cgtagcgctt gcgggccagc cgcgcgtagt cgcccagcgc ctcgacctgc aggcgctgcg
    20941 cctcggtgcg ttcggtagac ttctgcgtcg cgatgagcgc gtcgtccacg tccgcgaatg
    21001 cgccctgcac cgtcttctcg tagttcgcga gtagttgctc acgcctcgcg tcggcctgct
    21061 cgttcacgcc tacgttggag ccaccctgga agatcgggcc ggtcgcctgc ccggcgtagc
    21121 tccaggtgcc ggacccgccg tcaaacagct tgttgaggtc caggctggcc acgcccagca
    21181 ggcccgtcag cgatatggac gggaagtaaa gcgcgcgcgc cgcgctcacc cgcgcgttcg
    21241 cggcgatcaa cgactgctcg gccgaaacca cgtcgggccg ctggacgagg atgtccgagg
    21301 gcagtccagg tggcacgatg ggtgtgccga gttccagcag aacgcgtccg cgcgcgatcg
    21361 gccccgggtt ccgcccgagg agcagggata gagcgtcctc ctgccgtgcg atctgctcct
    21421 gcagcacggg caccgccgct gccgcgactt cgtactggta gcgcgcctgc gagagttcga
    21481 ccttcgagat gacgccgccc ttgtagcgct tttcgaagac gtcgagcgcc ttgcgccggc
    21541 tcgcgagtgt cgcctccgag gtgcggagct ggtcgtcgag cgacagcagc gtgaggtatc
    21601 ccgatgtcac cgcgctcacg agcgtgagca cggcggcgcg gcgatccgcg tcgctggcgc
    21661 gccagtccgc gctcgcagct gccgtctggt tgcggatctt gccccacacg tcgatctccc
    21721 agcccgcggt gaacccggcc gcgctggaat tcgccacgac gtcgttgccc ttgccgacca
    21781 cctgcgcgga cggcttgttc tggccgctgc tcgcggcgta gcccacgctc gggaaaatcg
    21841 gcgcgcgcgt cgtgaccatg gcgccgtgaa actcgtccac gcgagccgcg gcgatgcgca
    21901 gatcttcgtt gttgcgcaag gcctcgagca cgagttcgtc gagcaccgga tcgccgaaac
    21961 tcgaccacca ctggacatcg gccgggcgct gcgcctcgga caaggtgaag cggtactgcg
    22021 ccggaatgtc gagctttggg cgctggtacg gcgtgccgag gcaggcagtc agcgaccaac
    22081 aagcgacgag aagcactgcg cgtcgcatgg ctcagtggcc tcccggcccg gtggtggccg
    22141 tgtccccgcc gggagtggca ggcgcaggcg ccgccggagc cttcggcggg aagaagcgct
    22201 cgctcagctc atttatcact acgtagaaga gcggaacgaa gaacaacgca agcgtagtgg
    22261 cgccgaccat gccgccgatc acgccggtgc cgatcgagtg gcgcgaggcc gnnnnnnnnn
    22321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    22381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngtggcgccg accatgccgc cgatcacgcc
    22441 ggtgccgatc gagtggcgcg aggccgagcc tgcgcccgag gcgatggcaa gcgggatggc
    22501 gccggccgtg aaggccagcg aggtcatgac gatcggtcgc aggcgcgcct tcgcgccctc
    22561 gaccgcggac tcgacgatcg acaggccctt ctggcgctcg agcaccgcga actcgacgat
    22621 caggatcgcg ttcttggcgg cgaggccgat gagggtgacg aggccgatct ggaagtagac
    22681 gtcgttctcc atgccgcgca gccagaccgc gagcagcgcg ccgagcaagc cgaagggcac
    22741 ggccgtgatc acgctgcccg gcagcgacca cgactcgtac tgcgccgcga ggatcaggaa
    22801 caccatgacg aggccgaata cgaacaccat ggccgatgcg ccgcccgact gcttctcttc
    22861 gtacgcctgc ccggaccacg cgatgccgta accctccggc agcgtcgccg cgagcttttc
    22921 catggtggcg atggcctgcc ccgagctgta gcccggcgcc ggaccgccgt tgatcttcgc
    22981 ggccgggaag ccgttgaatc gcgggatcag gtccggaccc ttcgtgaagc gcgccgtggt
    23041 gaccgcgtcc aggggaacca tctcgcccga gcgtccgcgc acatagatgt tgcgcaggtc
    23101 ttcggggctg ttgcggaact cgggctcggc ctgcagcacg acctgccaca cgcgaccgta
    23161 cttgttgtac tggctcacgt acagtgagcc gaacagcgac tgcagcgcac cgtatacgtc
    23221 ggcaatcggc gcgccgagcg tctcggcctt ctcgcgatcg accttcacgc tcagctggcg
    23281 cgaggcgggg ttgaaggtcg agttcacgcc ggcgagcgca ggctgctgct ttgcgtcggc
    23341 gaggacctgc tgcaccgcct gggcgaggcg cgcgggctcg ccgcccgtca ggtcctgcac
    23401 ccacatctcg aagcccccct gcatgccgag gcccgggatc ggcggcgggt tgatcggtat
    23461 gacgtaggcg tcctcgatct gcgcgagctc cggcgcgatc gccgcgatca ttgcatcgag
    23521 cgacaggctc ctgtccttgc gctccgcgaa gtccttgagg ctcacgaaga ccgtggcgga
    23581 gttggtcttg tactggctgt cgatcgcgct gtacccggag agcacggacg cgtacttcgc
    23641 cgccgggtgc ttgagcacga tcccggcgat gcggcggccg acggcctcgg agcggtcgag
    23701 gctcgccgag tccggcagga tcgccgccac gatcatgaac ccctggtcct ccggcgggac
    23761 gaagctgccc ggcaccacct tgaacaggcc gaggatcgcc acgacgaacg ctgcgaatac
    23821 cgccagcgcg agggcggcgc gcttcaacac ccagcgcacg ccctgcgtgt aactctcggt
    23881 cagccgctcg aaccactcat tgaatttctg caggaccttc ggcgggtgac cgtgcgcgcc
    23941 gtcgagcagc aacgccgcaa gggccggcgt cagcgtcagc gccacgaagc cggacaggat
    24001 caccgagatg accaccgtca tcgcgaactg cttgtagagc actcccgtcg tgccgccgag
    24061 gaacgccgtc ggcacgaaga ccgcggcgag cacgagcacg atcgccacga ccgggcccga
    24121 cacctcgtgc atggctgcga acgcggcttc gcgcgcggag aggcccttgc ccttcatgtt
    24181 ggcctcgacc gcctcgatca ccacgatcgc gtcgtccacc acgatgccga tcgagagcac
    24241 catgccgaag agcgtgagca ggttcaccga gaagccgagc atggtcatgc cgacgaacgt
    24301 gcccacgatc gagaccacga cggcaatggt cgggatcagc gtcgcgcgca ggttctgcag
    24361 gaagacgtac accaccagca cgacgagcac gacggcctcg agcagcgtct tcacgacctc
    24421 gtcgatggac gccgtgacga acttggtggt gtcgagcgag acctcgtagt cgatgccggg
    24481 cgggaagctc ttcttcaggt tctcgagcgt cttgcgcacg ccttgcgcga cctgcagcgc
    24541 gttggagccg ggctgctggt acacggcgat gaagacggcc ggcttgccgt tcaggctcgg
    24601 ccgcatcatg tagttctgca ggccttcctc ggcccgcccg acgtcgccga gccgcacgat
    24661 ggccgcgccc tccgggtccg tgcgcaggat gatgtcgtcg aactgcgccg gctcgttgaa
    24721 gcgtccttcc gtgacgaccg gaacggtgac ctcgaccggg cgggaggtcg gcgactgccc
    24781 gatcgagccc gcgccgaact gctggttctg tttcgcgatc gcctgctgga tgtcggtgga
    24841 cgtgattcca cgcgccgcca tgcggtccgg gctcagccag atgcgcattg cgttgtccgc
    24901 ggcgccgaac aatgtcgcct ggttggcgcc cggcacacgc ttgacggcgt cgaggacgta
    24961 gaggttcgcg tagttggcga cgtagttgac gtcgtagcca ccgtcagggg catagacggc
    25021 gagcagcatc atgatgctgg acgaacgctt ctcgacccgg acgcccgtct tctgcaccac
    25081 gtcggggagc tgcggcagcg cgatgttgac gcggttctgc acctgcacct gcgcggtgtc
    25141 gg
//
LOCUS       DAOMFD010000011        24365 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_328_length_24365_cov_25.308680, whole genome shotgun sequence.
ACCESSION   DAOMFD010000011 DAOMFD010000000
VERSION     DAOMFD010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 24365)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24365)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24365
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_328_length_24365_cov_25.308680"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(232..1062)
                     /locus_tag="PL152_01380"
     CDS             complement(232..1062)
                     /locus_tag="PL152_01380"
                     /inference="COORDINATES: protein motif:HMM:NF004794.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HQR47953.1"
                     /translation="MDFANLTIERSGQVATLWLDRPDKLNALHRPLWSSIPAAVAALD
                     ADADVRAIVLAGRGKAFCAGIDLLDHAPGMAGGGSLSGKGDSAVGKRLALLDDIRRYQ
                     DTCSSFADTNKPVIAAVHGVCLGAGMDLITACDIRVASADAVFSVRETRIAMVADVGT
                     LQRLPRVIGDGPARELVFTGRDIDAARALAIGLVNEVLPDAVAAHARAQALAQEIAAN
                     SPLAVQGSKQVLSQARRREVAAELDYVALWNAAFLHSDDLAEAMTAFMQRRKPEFRGR
                     "
     gene            complement(1078..1914)
                     /locus_tag="PL152_01385"
     CDS             complement(1078..1914)
                     /locus_tag="PL152_01385"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR47954.1"
                     /translation="MSTPGLTFRDRNVLVTGASSGIGRETALAFAAAGANVVLVARRP
                     KALAAVATQARKSGVQALAAAADVTKPAAVTAVFQKAIKRFGSVDIVVNNAGVLIPAE
                     VATMKDADLHSMLDVNLFGALHVMQDAVKAMRKQGRGHIVNVASLAGRRGLSPLGGYC
                     ASKFALIGLTEALRTELVGERIHVSMVLPGVVDTPMAAAISQDTEHGGYWPQALNMPP
                     SWVVWSIFLAIRFRLAEVGVPPGSAMLEKLASLAPGATDTLLRWASDAARRVGKPTRA
                     SR"
     gene            complement(2082..3197)
                     /gene="ald"
                     /locus_tag="PL152_01390"
     CDS             complement(2082..3197)
                     /gene="ald"
                     /locus_tag="PL152_01390"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HQR47955.1"
                     /translation="MRIGVPKEIKVHEYRVGLIPAAVRELVESGHEVTVQTGAAAGIG
                     FTDADYQRVGAKIAPTAEEIFAKAEMIVKVKEPQPVECAQLKAGQVLYTYLHLAPDPQ
                     QTAALVNSGATCIAYETVTAPDGSLPLLTPMSEVAGRMSVQVGAYCLQKANGGRGILL
                     GGVPGVAPAKVVILGGGVSGTHAAEMAVGLRAEVTVVDRSVKRLRELSSIFGPALRTE
                     YSTQEHIDALVQDADLVIGAVLIAGAAAPKLVTREMVRRMKAGSVLVDISIDQGGCFE
                     TSHATTHAEPTYVVDDVIHYCVANMPGAVPRTSTFALNNATLPFARKLADLGWKEAMR
                     QDPHLANGLNVHAGHITNEAVARDLGYDYKPVAQFLK"
     gene            3331..3795
                     /locus_tag="PL152_01395"
     CDS             3331..3795
                     /locus_tag="PL152_01395"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQR47956.1"
                     /translation="MKLDRTDQHILAELQRDGRLSNRDLAEKVHLSESACLRRVRSLE
                     EAGVIDRYTALVTQAKVGLPGNVFVSITLNRQEQGDLAAFEEAVKRVPEVMECYLMTG
                     QQDYLLRVVVSDPADFERLHSQHLTRLPGVARVQSSFALRTVRKSTELPVHG"
     gene            3861..4844
                     /locus_tag="PL152_01400"
     CDS             3861..4844
                     /locus_tag="PL152_01400"
                     /inference="COORDINATES: protein motif:HMM:NF025034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl protease family protein"
                     /protein_id="HQR47957.1"
                     /translation="MAGPHDLQAQVPPPATTAPAAAPAASAPGEGEPLYAAPTREDRS
                     GRILAAVEIDGQGPYRFILDTGANSSALAPQVAEQLRLPSAGDVSVHGVTGSAMVPAV
                     QVATMRAGDVVQSAVILPVLPGDIFGGADGILGISGMQDMRIEVDFLHDRVTISRSRG
                     WGATSSLVTIKASLWQGGLLLVKGRVGSIPTQIIIDTGAERTLGNVPLRQAIVARTRP
                     GEEFSTTVFGATPDTEAGTYFRAPRISIGSSHLNDQPVTFGDLHVFELWGLTDVPALV
                     IGMDVLGRFEKFVVDYRRKEFQISSRSNGKAFMRKCTVTDCGSRIPGSDGD"
     gene            complement(4890..6383)
                     /locus_tag="PL152_01405"
     CDS             complement(4890..6383)
                     /locus_tag="PL152_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR47958.1"
                     /translation="MSPAVGPASTPPQSASDWLALSRRHEQRGEMSPMFVAAQRALQL
                     EPASLDARFREIECLIVFGRTRVALQRLVALEPDCAGDAVRMLQAGAVYSRLNRIPDY
                     HRCYARAFDLARDDRNARLNLARSFIVLGDLGRAQELLEDAIRAWPQDHEAWHALARL
                     RKWTADDNHVETLERLAGNATDPASKVAFCYALHKELEDLGEDERAMQWLKLGARTLR
                     GTLSYRPETDAAVMESIARNFPASRVRAAPRTGLGLGAIFVIGLPRSGTTMVDRILSA
                     HRKVESLGELRDLTYAVMTGGGTVASSPQAPAARPAAAGMQAIGRCYMDAVATYRGDR
                     PCFVDKAPMNFLYAGLIRLALPGARIVLLRRDPMDSCLAIHKTLFREGSPFACDLEDL
                     GRYYLAWHRLAEHWTEALGDAMLTVEYESLVRNQEAETRRMLGYCGLEWDPACLDFHL
                     NASPTATASAAQVRQPLYSTSIGRWKRHAADLEPLARVLTGAGIAIG"
     gene            6665..7174
                     /locus_tag="PL152_01410"
     CDS             6665..7174
                     /locus_tag="PL152_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47959.1"
                     /translation="MSDDAARLACYDAIFRASGQAVVPGAAPAATPGSVQQASTATAT
                     ATAAAASAQPPAAAAPPRNPEQDFGLTPARKESLEAAKAKESATPPPPKEPAAPESMT
                     ARVTTAAHRLTGELVLILDNGQVWVQIDTETKARVKEGDEVTIRKATLGSYFLVAPNH
                     VLVRVRRVK"
     gene            complement(7241..9247)
                     /locus_tag="PL152_01415"
     CDS             complement(7241..9247)
                     /locus_tag="PL152_01415"
                     /inference="COORDINATES: protein motif:HMM:NF012888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR47960.1"
                     /translation="MSTASNAVDALRSAIEGAARLVASDPERAAEQAGEILKQAPDHA
                     AARLVLGAAHRSRGDAPSALAVLEPLAAARPDWVAARYELGLAYGLAGRSADAVAELR
                     RALEIRPDMADAWRAVAFHLYALGDRAGADSAYLRLIDAAAQDGRLAGAADALAEGRY
                     ADAEPVLVAHLARVPQDVAALRMLAELAARTGQYTRAEILLEHCLDLAPGFRAARLNL
                     AQVMLWQARFAESLRIVELMLQEKPDDVPLRTLRAELLGRLGDNAGSLEILRQLLQEN
                     PADAVVWHGYGHALKTAGQPVESADAYRRTISLAPQFGEAYWSLANLKTFRFTAEDTD
                     RMLRQVERADLSVEDRFHFHFALGKAYEDAGDYERSFRHYAEGNRLRRSVVDYDAAAE
                     ADRVRRAAGVFTREFIASRSGRGCPAADPIFIVGMPRAGSTLIEQILASHSAVEGTME
                     LPDIGAIARELGQRGGNTQRSAYPEIVRDLDDAELHRLGERYLETTRAQRKGALPHFI
                     DKNPRNWLHVGLIRLILPNARIIDARRHPMSCCFSVFKQHFAHGNRFSYDLAELGAYY
                     RNYVDLMAHFDAAMPGHVHRVIYERLVTDTEAEVRRLLEYCGLPFEDACLRFYENKRA
                     VRTASAEQVRRPIYRDALEQWVHYRPWLGALEQALGPAVDTYEQ"
     gene            complement(9399..12524)
                     /locus_tag="PL152_01420"
     CDS             complement(9399..12524)
                     /locus_tag="PL152_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR47961.1"
                     /translation="MANRNIERAVRVALLASAVSAGAYSAGALAQEAGATPDEVEQII
                     VTGSRIPQPNLEGASPVTQIGSQNVELQGITDSVNLVNQLPQAFAAQSSTVSNGSSGT
                     STIDLRNLGSSRTLVLVNGRRLPAGDPASISGIAPDINFIPAPLINRVEVLTGGAGAV
                     YGSDAISGVVNFIMKDDFEGIELGGQYSLYSTNNSDGDNANLMDGILAKRKFAKPGNS
                     VTDGDTYDLSLMMGSNFADGKGNATMFVGYRHTDPVLQGDRISSACALQTAYIETDPE
                     ILAYYSITASSAYFYGGAPIGGSWNKKKAVRCGGSGTRANGSFYAAGNNFTGPYGVTP
                     TGPARDSSLYNYAPLNYYQRPDERWNADVFMHYDVTDQIQAYGQFMFMDDHTVAQIAP
                     SGIFSTYYDLPCDPTAANANPLVSQQWLNYLCYNNATPITTGTAPVFIQRRNVEGGPR
                     QDDLRHTDYQGVLGIRGKIWDHWNYDLFGQYGTTILNEHVNNYVSNQRALAALNAVAN
                     PDYDPNLPFDAVSNNPAACAGALAGYAAGCVPWDIWSANTATTGVNAAALNYINVQGF
                     SSGSTAQTVVNGSLSADLGDYGVKMPWAASGIGVAFGVEYRQEDLQLDYDTENQTGDL
                     GGGSGVLLPVKGGYNVTDVFMEARVPIVEDAPGAKSLTFNGSYRYSDYSTGVNTNTYG
                     LGLDWAPMDDFKLRGSYQHAVRAPNVLELFTPQAVGLFDWAGDPCAGSNPSASPAECA
                     LTDNDNGNTAAWYGTTPVSPAGQYNALFGGQSTLKPETGNTWTVGLVLTPTFLEGFTG
                     SIDYWNIKIEDVISSIAPATTVANCIAFADPFFCDNIHRNAGGSLWTTKDGYVTALTS
                     NLGFYHTSGTDFNLNYNLPIGDWGSLNFNELATWVDSFEIQEIPGYGKYDCKGFYGPS
                     KCTTPTPEWKSKFQTVWSTPWNVDISVAWRYIDSVELETYSKDPDLQGSYDTQKPFDK
                     WLGSQNYIDLGATYTFAEHYTINAGINNLLDQDPPLSGSLPTSPANGNTYPGVYDALG
                     RYMFVGLTAKF"
     gene            complement(12881..14161)
                     /locus_tag="PL152_01425"
     CDS             complement(12881..14161)
                     /locus_tag="PL152_01425"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HQR47962.1"
                     /translation="MNASVNAGIPAMAQQARELARVGRAAEAALVWQRVLGADPNHTE
                     ALLALATQALARRDATLALQMLQHAAATAPRDPMVQIYQAVAFRDLGNPQEEMAAVTR
                     ALAIDPYFFPALLHKATLLERLGKRRQAARVYKDVLKIMPAKDQAAPAFARAVEHAQA
                     VVDENRGSLERHLDGVLADLRRRHSTARLDRFEETVAVMTGAKKRYSPEPVMLYFAQL
                     PPLQFYPNELFPWIPELEAATEDIRRECEAVVAEQRDEFDPYIQRPPGAPLDQWKDLN
                     HSPRWATYLLWQHGQRIDAHCERCPRTAEVVESLPLARTPNFSPNVLFSALEAHTTIP
                     PHNGDTNVRLIVHLPLILPPGCTFRVGNDTREWQYGKAWVFDDSVDHEARNDSDQLRV
                     QLMLDVWNPFLTDVEKELVNGLLNGQRTYYAAED"
     gene            complement(14273..14827)
                     /locus_tag="PL152_01430"
     CDS             complement(14273..14827)
                     /locus_tag="PL152_01430"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HQR47963.1"
                     /translation="MSQPTPRLLASLLARAASIALPFAWCLVPAAARADVAQDDLIPL
                     AQFPREPLVVETHSARRRTFDAWRADTPATHEQGLMFVREMRDGQAMIFVYDPPRYVA
                     MWMKNTLIPLDMLFVNADGCVIKVKHDAQPQSLATIAADGPVALVVELKGGAASALGI
                     GLGDRVRRPQAGWPTSDRACTSDP"
     gene            complement(14824..15474)
                     /locus_tag="PL152_01435"
     CDS             complement(14824..15474)
                     /locus_tag="PL152_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47964.1"
                     /translation="MASLALALLVTGCGHSHDDRSADSAGTAHVSGQPDVTPRPASGG
                     ETSEVARTPGAASGASMPSGQQALCIPANEGSLEARLHGAIDAEIRWAYPMPQCRGGV
                     RPDGVGVRLLYKGEIPGQGPLLVVIGMGPLRAGENATSVPANITLVREGSGRFFATQG
                     DDKCSVDEVQQVPVFGNGHTYRITGRGYCTQPARAVGGEGVVVVSRFDLSAVVDFQ"
     gene            complement(15497..16405)
                     /locus_tag="PL152_01440"
     CDS             complement(15497..16405)
                     /locus_tag="PL152_01440"
                     /inference="COORDINATES: protein motif:HMM:NF025168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing protein"
                     /protein_id="HQR47965.1"
                     /translation="MTAQTPRTVEQYLDALRAALKGADPALVQDALYDAEEHLRAELA
                     ARAGQDEAIVLESIVSSYGAPDEVADAYRSNEATIQKALRTPAPRAHQSTAGRFFGVY
                     VDPRAYLSLLYMLLTLVTGIFYFTFAVTGLSLSVGLAILIIGVPFFLLFVGTTRIVAL
                     AEGRLVEAMLGTRMPRRPPYPDRETPFLRRIGDMLKDPRTWGTLVYFLLMLPLGIAYF
                     TFVVVGVAVSVATTVAPIAVLLYHAGVITIDGDVNVHSPHPALLPLISIVGVLLLTVT
                     LHLARGIGYLHGLLAKNLLVRPPGVD"
     gene            complement(16402..16812)
                     /locus_tag="PL152_01445"
     CDS             complement(16402..16812)
                     /locus_tag="PL152_01445"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HQR47966.1"
                     /translation="MAIDPVDDAPVGDAEPLIRKFQKELTAGLTALVLLSVLAETEGD
                     LYGYEIAKRLSGDAAPASPFKQGALYPVLRNLSAAGLLSSRVVPSYAGPPRRYYRITP
                     LGRDVLKSWCATWTETRSFVDSIVKTTTAGEATP"
     gene            17143..18420
                     /gene="glgC"
                     /locus_tag="PL152_01450"
     CDS             17143..18420
                     /gene="glgC"
                     /locus_tag="PL152_01450"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HQR47967.1"
                     /translation="MSTEQRQFAPRRYLSRLTRGTLAVIMAGGRGERLKQLTEDRCKP
                     ATPFGGKFRIIDFVLSNCVNSGIRQISVLTQYKAHSLIQHIERGWSYLRGEFGEFVEI
                     IPAQQRHGLAWYQGTADSLWQNMDIVKSHRPLHVLVLAGDHIYKMDYGPMIGLHVENE
                     ADITVGVVEVPVERAREFGVLTVDESNRVLRFQEKPLDPEPVPGRPDVALASMGIYVF
                     NPKLLERLLRADADDPNSAHDFGRNIVPGAIDKLRVFAYPFEDIRTKAQNYWRDVGTV
                     DAYYDANMELVRVAPELNIYDEQWPIWTYQEQLPPAKFVFDDPDRRGCAIDSMVSGGC
                     IISGSHVSNSLLFSNVRVHDWSSIDRSVVLPRVRVGERCVIRGAIIDEGAIIPDGTHI
                     GVDPVADAERYYVTEGGVALVTSAMLRGASKAG"
     gene            18456..18941
                     /locus_tag="PL152_01455"
     CDS             18456..18941
                     /locus_tag="PL152_01455"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HQR47968.1"
                     /translation="MPWIPPKRALPLAVVLAMLAACSTLTPKLETPTVSLLGVQMTSG
                     DMFSQRFNVRVKIENPNDLEVPVKSLEYTIYLMGDRFGEGTSERPFVLPARGEAEFDM
                     LVTTDFVSALGRLVSRMGGNKLENVEYAVTGKLHLDKGFVRTIPFEHKGLLNFTKAPK
                     N"
     gene            complement(18986..20437)
                     /locus_tag="PL152_01460"
     CDS             complement(18986..20437)
                     /locus_tag="PL152_01460"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HQR47969.1"
                     /translation="MRICFIAAEYSPFAKTGGLGDVAAALTRYLARRGHDLRVFVPLH
                     GQMELAGIDRHPVDFLRDLTLRVGPHEYRYDVQTARVPDTQLWVYLVDCPALFDRPRI
                     YSDAPDEHVRYLALTRAAFDCCQRMGFAPEIMHSNDWHTAFAPLLLKHAYAWDLGLFA
                     ATRSVFTIHNIGYQGVFAASHAADLGPGVPIAALHQPDLAAGHINPMRHGVIHADAVT
                     TVSPTYAAEICTPAGGYGLDPDLRARGDSLTGILNGVDYDEWSPSTDRYIPHRYDATD
                     LTGKLWNKQALLDLLRMPATPDTPLVGMVTRLTQQKGIDLLFDVLPELLRTRNLRFVA
                     LGNGDHQYEEFLHGLQQSFPGRAVFYSGYSEELAHMIEASADIFLMPSMYEPCGLNQM
                     YSLKYGTVPVVRRTGGLADSVQHFDPASGQGTGIVFNDFDASGVRWALETALEWYGWP
                     TVWRRLVQNGMRQDFSWDTQGAEYERLYARLKK"
     gene            20689..21567
                     /locus_tag="PL152_01465"
     CDS             20689..21567
                     /locus_tag="PL152_01465"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HQR47970.1"
                     /translation="MNTAPAAPSDRPVPLDYRRIERAIRYIDRRFRDQPSLEEIAKSA
                     GLAPHHFARVFRRWAGISPKQFVQHLTLEAARDSLGRDESVLQAALDAGLSGPGRLHD
                     LFVTLEAATPGDFKARGRGMTIRYGTATTPFGVATIAQTSRGIGFVGFELARGEVPGW
                     REFRDTWRHAEFVADDAVAQDLARKVWGEGAQAGESLRLWVHGSNFQIQVWQALLRCA
                     RDTTVNYADVAREIGRPEAARAVGGAVGANPVAWLIPCHHVLRTSGALGGYRWGVERK
                     RAMLAWELSRTVAAQG"
     gene            complement(21618..22103)
                     /gene="phaR"
                     /locus_tag="PL152_01470"
     CDS             complement(21618..22103)
                     /gene="phaR"
                     /locus_tag="PL152_01470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HQR47971.1"
                     /translation="MSQERLIKKYANRRLYDASQSRHITLDDIRALIVKGEKIKVVED
                     KTGHDITRHILLQVIAEQEQFGRPILSTKVLEAIIRFYGNAMQGFLAGFLEKSIETFL
                     NQQEALQAQISKIVSSTPLSTVADITRQNLETLAKAQESLLGAILPRRDKSRDEDDKR
                     G"
     gene            22275..22748
                     /locus_tag="PL152_01475"
     CDS             22275..22748
                     /locus_tag="PL152_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HQR47972.1"
                     /translation="MSQQELFERLTSQIGEEIYVGEWHQVTQEMIDAFAVVTGDHQWI
                     HVDVERARRESPWRSTIAHGFLTLSLLPMLRGMGSGQIPYPGVKQGVNYGLDRLRFPN
                     VVKAGARVRARCRLLKVEQIPGGLQVTEQFTAEIEGEQKPGCVADTIVRLYFQTA"
     gene            22842..23783
                     /gene="cysK"
                     /locus_tag="PL152_01480"
     CDS             22842..23783
                     /gene="cysK"
                     /locus_tag="PL152_01480"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004124 - cysteine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQR47973.1"
                     /translation="MIYDSILQTIGRTPVVRVNRIAPAHVTMYVKCEFFNPLASVKDR
                     LAIAIIEDAERSGALKPGQTVVEATSGNTGIALAMVCAAKGYPFVSVMVETFSIERRK
                     IMRMLGAKVILTPAAERGSGMVRKAAELARKHGWFLARQFENEANPAYHRQTTGPEIL
                     SDFAGRRLDFFVSGWGTGGTLTGAGEMIRLARPEVRIVATEPAGAALLSGAEFKPHKI
                     QGWTPDFVPAVLNRSVPHEIVLVTDDEALAAARSLASREGIFCGISSGGTFAAALKVA
                     EKAPQGSVILAMLPDTGERYMSTPLFEGIAEGSDPEP"
BASE COUNT         3866 a         7989 c         8495 g         4015 t
ORIGIN      
        1 gccgcgagca gctgaccggc tacaagaagc cgaagttcat cgagttccgc accgaactgc
       61 cgaagacgaa cgtcgggaag atcctccgcc gggaattacg tgactcgaag gccgcgtgaa
      121 aaacagggga cattctccta ttttccgggc cctgcgcgag gcctgggaaa acggggtctg
      181 gccccgtttc cgaaattgga gaatgtcccc tgttttcccg attttcgatt ttcagcggcc
      241 cctgaactcg ggcttgcgcc gctgcatgaa cgcggtcatg gcctcggcga gatcgtccga
      301 gtgcaggaac gcggcgttcc acagcgccac gtaatcgagt tccgcggcga cctcgcggcg
      361 gcgcgcctgg ctcagcacct gcttcgatcc ctgcacggcg agcggtgaat tcgcggcgat
      421 ctcctgcgcg agcgcctgcg cccgggcgtg cgccgcgact gcgtccggca gcacctcgtt
      481 caccaggccg atcgccaggg cgcgcgcggc atcgatgtcg cggccggtga acaccagctc
      541 gcgcgccggt ccgtcgccga tgacgcgcgg caggcgctgc agcgtgccga cgtcggcgac
      601 catcgcgatg cgcgtctcgc gcaccgagaa gacggcatcg gcggacgcga cacggatgtc
      661 gcaggccgtg atcaggtcca tgccggcccc gaggcacacg ccgtggacgg ccgcgatcac
      721 gggcttgttg gtgtccgcga agctgctgca cgtgtcctgg tatcgacgga tgtcgtcgag
      781 gagcgccagg cgcttcccga ccgccgagtc gcccttgccc gagagagagc cgcctccagc
      841 catgccgggg gcgtgatcga gcaggtcgat gcctgcgcag aatgccttgc cacgccccgc
      901 gagcacgatc gcgcgcacgt cggcgtcggc atccagagcc gcgaccgccg ccggaatcga
      961 gctccagagc gggcgatgca gcgcattgag cttgtccggc cggtcgagcc acagcgtcgc
     1021 gacttggccg gagcgttcga tcgtcaggtt ggcgaaatcc atgtcattcc tctcggctca
     1081 acgggaagcg cgcgtgggct tgccgacgcg acgcgcggcg tcgctggccc agcgtaacag
     1141 ggtgtcggtc gcgcccggcg ccagcgaggc cagcttctcg agcatcgcgc tgcccggagg
     1201 cacgccgacc tcggcgagcc ggaagcggat cgcgaggaag atggaccaca cgacccagga
     1261 tggcggcatg ttgagcgcct gcggccagta gccgccgtgc tcggtgtcct gcgagatcgc
     1321 ggcggccatc ggcgtgtcca cgacgccggg cagcaccatc gatacgtgga tacgctcgcc
     1381 caccagttcc gtgcgcagcg cctcggtgag cccgatcagc gcgaacttgc tggcgcagta
     1441 acctccgagg ggtgacaggc cgcgacggcc ggcgagcgag gcgacgttca cgatatggcc
     1501 gcgaccctgc ttgcgcatgg ccttgacggc gtcctgcatg acgtgcagcg cgccgaacag
     1561 gttcacgtcg agcatcgagt gcaggtcggc gtccttcatc gtcgcgacct ccgcggggat
     1621 gagcactccc gcgttgttca ccacgatgtc cacggacccg aagcgcttta tggccttttg
     1681 aaatacagcg gtgaccgccg ctggcttggt gacgtcagcg gcggcggcga gtgcctgcac
     1741 gccgctcttg cgggcctgcg tggcgaccgc ggccagcgcc ttggggcgcc gggcgacgag
     1801 cacgacgtta gcacccgcgg cggcgaatgc gagcgcggtc tcgcgcccga tgccggagga
     1861 cgcaccggtg acgagcacat tccggtcgcg gaacgtaagt cctggcgtcg acatgggagg
     1921 cacctcgtgg aagacgtgcg agccgggcgg gcgggggtgc cacaagggta acggaaaaag
     1981 gggacattct cccttttcgc ttccgcgaaa agggagaatg tccccttcaa tccgcatcgg
     2041 ggcccggtgt cgctccccgg ggcctggccc cgtttgctcg tttacttcag gaactgggcg
     2101 acgggcttgt agtcgtagcc gaggtcccgc gccacggcct cgttggtgat gtgtccggca
     2161 tgcacgttga ggccgttggc gaggtgcgga tcctgccgca tggcctcctt ccagccgagg
     2221 tcggcgagct tgcgtgcaaa tggcagggtg gcattgttca gcgcgaacgt ggacgtgcgc
     2281 ggcacggcgc ccggcatgtt cgccacgcag tagtggatga cgtcgtcgac gacgtacgtc
     2341 ggctcggcgt gcgtggtcgc gtggctggtc tcgaagcagc cgccctggtc gatcgagatg
     2401 tcgaccagca cggagccggc cttcatgcgc cggaccatct cgcgagtgac cagcttggga
     2461 gcagctgctc ccgcaatcag caccgcgccg atcaccaggt cggcgtcctg caccagcgcg
     2521 tcgatgtgct cctgcgtcga gtattcggtg cgcaacgcag gcccgaagat cgaggacagt
     2581 tcgcgcaggc gcttcaccga gcgatcgacc acggtcacct cggcacgcag tccgaccgcc
     2641 atctcggccg cgtgggtgcc ggagacgcca ccaccgagga tcacgacctt ggccggggcc
     2701 actcccggca cgccgccgag cagtatgccg cggccgccat tggccttctg caggcagtac
     2761 gcgccgacct gcacggacat gcggcccgcg acctcgctca tcggcgtcag caaggggagc
     2821 gatccgtccg gcgccgtgac ggtctcgtag gcgatgcagg tggcgcccga attcaccagc
     2881 gccgcggtct gttgcgggtc cggtgccagg tgcaggtacg tgtacaggac ttgtccggcc
     2941 ttcagctgcg cgcactcgac gggctgcggc tccttgacct taacgatcat ctcggccttg
     3001 gcaaagatct cctccgccgt gggcgcgatt ttcgcgccga cgcgctggta gtccgcatcg
     3061 gtgaaaccga tgccggcggc ggctcccgtc tggacggtga cttcgtggcc gctttccacg
     3121 agttcacgca cggcggccgg gatcaggccg acacggtact catggacctt gatttcctta
     3181 ggcacgccga tgcgcatgga cgctctcccc tggattgaat ccgcggagca taccgcgcgt
     3241 gtcgggcagg aaaatgcaaa agtcgtcgac tgcggccata tagacgcaat aaaatgcggc
     3301 gaaagcttga atagccgaag gatcctgcgc atgaagctcg accggaccga ccagcacatc
     3361 ctcgcggagt tgcagcgcga cggccgcctc agtaatcgcg acctcgccga gaaggtccac
     3421 ttgagtgagt ccgcatgcct gcgacgggtg cgcagcctgg aggaggccgg cgtcattgac
     3481 cgctacactg cgctcgtgac ccaggcgaag gtcggcctgc cgggcaacgt attcgtcagc
     3541 atcacgctga accggcagga gcagggcgat ctcgccgcat tcgaggaagc ggtcaagcgt
     3601 gttcccgaag tcatggagtg ctacctgatg accggccagc aggactacct gctgcgcgtc
     3661 gtcgtgtccg atcccgcgga cttcgagcgg ctgcacagcc agcacctcac gcgactgccg
     3721 ggcgtggcgc gcgtgcagtc gagtttcgcg ctcaggacgg tgcgcaagag cacggaactc
     3781 ccggtgcacg gatgacgccc acacgtcgat tcgcatggcg cgcatcggga gccgcggcga
     3841 tggctgcggc tcttgccacg atggcagggc cccacgacct ccaggcgcag gtgccgccgc
     3901 cggcgacgac cgcacccgcg gctgctccgg ccgcatcggc acccggtgaa ggcgagccgt
     3961 tgtacgcggc gccgacgcgc gaggatcgct cgggcaggat cctcgcggcg gtcgagatcg
     4021 acgggcaggg accgtatcga ttcatcctgg acacgggcgc caacagttcg gcgctcgccc
     4081 cgcaggttgc cgaacaactg cggctgccgt cggccggcga tgtcagcgtc cacggcgtga
     4141 ccggatccgc gatggtacct gccgtgcagg tcgcaacgat gagagccggg gatgtcgtgc
     4201 agtcggcagt gatccttccg gtgctgcctg gagacatctt cggtggcgca gatggcatcc
     4261 tcgggatctc cggcatgcag gacatgagga tagaggtcga tttcctgcac gatcgcgtca
     4321 cgatcagccg gtcgcgcggc tggggggcga cttcgagcct ggtcacgatc aaggcgtccc
     4381 tgtggcaggg cgggctgctg ctggtcaagg gacgcgtcgg aagcattccc acgcagatca
     4441 tcatcgacac gggcgcggaa cgcaccttgg gaaacgtgcc gctccggcag gcgatcgtcg
     4501 ctcgcacgag gcccggggaa gagttctcga cgacggtgtt cggggccaca cccgacaccg
     4561 aggcggggac ctacttccgg gcaccgagga tttcaatcgg ctcgagccac ctgaacgacc
     4621 agcccgtcac cttcggcgac ctgcacgtgt tcgaactctg gggcctgacc gacgtgcccg
     4681 cgctggtgat cggcatggac gtgctcgggc ggttcgagaa attcgtcgtg gattaccgcc
     4741 gcaaggaatt ccagatcagc tcacgcagca acggcaaggc cttcatgcgc aagtgcaccg
     4801 tcacggactg cggttcgcgc attccagggt cggacggcga ctgaccggcc gcgcggcggc
     4861 gccggtccgc gcgcaatcgc ggtgcgcctt cacccgatcg caatcccggc cccggtgagg
     4921 acgcgcgcga gcggctcgag atcggctgcg tgccgcttcc agcgcccgat cgacgtcgag
     4981 tacagcggct gacgcacctg cgccgcgctc gcggtcgcgg tgggcgacgc gttcaggtgg
     5041 aagtcgaggc acgccgggtc ccactcgaga ccgcagtatc cgagcatgcg gcgcgtctcg
     5101 gcttcctgat tgcggaccag cgactcgtac tccaccgtca gcatggcgtc gcccagcgcc
     5161 tcggtccagt gctcggcgag cctgtgccag gccaggtagt agcgcccgag gtcctcgaga
     5221 tcgcaggcga acgggctgcc ttcgcggaac agcgtcttgt ggatcgcgag gcagctgtcc
     5281 atcggatcgc gtcgcagcag cacgatccgc gcgcccggca aggccagccg gatcagcccg
     5341 gcgtacagga aattcatcgg cgccttgtcc acgaagcagg ggcggtcgcc gcgatacgtc
     5401 gcgacggcgt ccatgtagca gcgtccgatg gcctgcatgc cggccgcggc tggacgagcc
     5461 gccggagcct gcggtgaact ggcgacggtg ccgccaccgg tcatcacggc gtaggtcagg
     5521 tcgcgcaact cgcccaggct ctcgactttg cgatgtgccg acaggatgcg gtcgaccatc
     5581 gtcgtgccgc tgcgcggcag gccgatgacg aagatcgccc cgagtccgag tccggtccga
     5641 ggcgctgcgc ggacgcggct ggccgggaag ttccgtgcga tggactccat gacggccgcg
     5701 tcggtctcgg ggcgatagct caacgtcccg cgcagggtcc gggcgccgag cttcagccat
     5761 tgcatcgccc gctcgtcctc gccgaggtcc tcgagctcct tgtgcagcgc gtagcagaat
     5821 gccaccttgc tcgcggggtc cgtggcgttg ccggcgagcc gctccagggt ttcgacgtga
     5881 ttgtcatccg cggtccactt gcgcaggcgc gccagcgcgt gccacgcctc gtggtcctgt
     5941 ggccaggcgc gaatggcgtc ctcgagcagt tcctgcgcgc gcccgaggtc gcccaggacg
     6001 atgaacgacc gcgccaggtt caagcgggca tttcgatcgt cgcgcgccag gtcgaacgca
     6061 cgcgcatagc accggtggta gtccggtatg cgattgaggc gcgaatacac ggcgcccgcc
     6121 tgcagcattc gcaccgcgtc gccggcgcag tcaggctcca gggccaccag tcgctgcagc
     6181 gcgacacgcg tgcggccgaa gacgatgagg cactcgattt cccggaagcg cgcgtcgaga
     6241 ctggctggct cgagttgcag cgccctctgc gccgccacga acatcgggct catctcgccg
     6301 cgctgctcgt ggcgacggct cagcgccagc cagtcagacg cggactgcgg tggcgtgctg
     6361 gccggaccaa cggctggcga catgagcgac cccggtgcaa gattagcgtg cgagtataca
     6421 ggcgctgctg ttcccggacg ccccggtacg acctgcccgc ggcaatcgcg ttgcggtagc
     6481 tctcgcggcg gatgaaaccg atactatagc gaccgcgccg gtcgtcccgc ggcgcgccgt
     6541 gatgcgaggg gtgcaggtcg tgcgtcgtgc cagtgcaagt gcgtttgtcg ccgcattcgc
     6601 ggtcatcgtg tcaccgggcc cggcatctgc ctccgacact gaacaggcgt tgcgctgcgc
     6661 ggcgatgagc gacgatgccg cccgcctcgc gtgctacgac gcgattttcc gggcctcggg
     6721 ccaggccgtc gtgcctggtg ccgccccggc cgcgacgccc gggagtgttc aacaggcgtc
     6781 gactgcgact gcgaccgcga ccgcagctgc ggcctcggcg cagccgcccg cggccgcggc
     6841 gccaccgcgg aatccggagc aggacttcgg cctcacgccc gcacgcaagg aatccctgga
     6901 ggccgccaag gccaaggaat ccgcgacacc acctccaccc aaggagccgg ccgcaccgga
     6961 gagcatgacg gccagggtca cgacggcggc gcaccgcctg accggtgagc tcgtgctgat
     7021 cctcgacaat ggccaggtct gggtacagat cgatacggag acgaaggcgc gcgtcaagga
     7081 aggcgacgaa gtcacgatcc gcaaggcgac gctcgggtcg tatttcctcg tcgcgccgaa
     7141 tcacgtcctg gtgcgcgttc gtcgcgtgaa gtagcgcggt cgagcagcac ggctgacgaa
     7201 ccgacgcgca cggggccggc cgccgcggtc cagccgcacg tcactgttcg taggtatcca
     7261 cggcggggcc cagggcctgc tcgagtgcgc cgagccaggg ccggtagtgc acccactgct
     7321 cgagcgcgtc gcggtagatc ggccggcgca cctgctcggc actcgcggtc ctgaccgccc
     7381 gcttgttctc gtagaagcgc aggcaggcat cctcgaacgg cagcccgcag tactcgagga
     7441 ggcggcgcac ttccgcttcg gtgtcggtca ccagtcgttc gtagatcacg cgatgcacgt
     7501 ggcccggcat ggccgcgtcg aagtgcgcca tgaggtcgac gtagttccgg tagtacgccc
     7561 cgagctccgc gaggtcgtag gagaagcgat tgccgtgcgc gaagtgctgt ttgaacacgg
     7621 agaagcagca gctcatcggg tgccgccgcg cgtcgatgat ccttgcgttg ggcaggatca
     7681 ggcggatcag gccgacgtgg agccagttgc gcgggttctt gtcgatgaag tgcggcaacg
     7741 cgcccttgcg ctgcgcgcgg gtcgtttcca ggtagcgctc gccgagcctg tgtaactcgg
     7801 cgtcgtcgag gtcgcgcacg atctcgggat acgcggaacg ctgggtgttg ccgccacgct
     7861 ggccgagctc gcgtgcgatg gcgccgatgt ccggaagctc catcgtgccc tcgacggcgg
     7921 aatggctcgc caggatctgc tcgatcagcg tcgatccggc gcgaggcatg cccacgatga
     7981 agatcgggtc ggccgccggg catccgcgtc cggagcggga cgcgatgaat tcgcgcgtga
     8041 acacgccagc cgcacgccgc acccggtcgg cctcggcggc ggcatcgtag tcgaccacgg
     8101 agcggcgcag acggttgcct tccgcgtagt gccggaagga gcgctcgtag tcaccggcgt
     8161 cctcgtacgc cttgccgagc gcgaagtgga agtggaagcg atcctcgacc gacaggtccg
     8221 cacgctcgac ctggcgcagc atgcggtcgg tgtcctccgc cgtgaaccgg aacgtcttca
     8281 ggttggcgag gctccagtac gcctcgccga actgcggcgc gaggctgatc gtccggcgat
     8341 aggcgtcggc ggattccacg ggctgccccg cggttttcaa tgcgtgcccg tagccgtgcc
     8401 acacgaccgc gtcggcgggg ttctcctgca gcaattggcg caggatttcg agggaccccg
     8461 cgttgtcccc gaggcggccg agcaattcgg cccgcaacgt gcgcagcggc acgtcgtcgg
     8521 gcttctcctg caacatcagc tcgactatcc gcagcgactc ggcgaagcgc gcctgccaga
     8581 gcatgacctg cgcaagattg aggcgtgccg cccggaagcc cggcgcgaga tcgaggcaat
     8641 gttccagcag gatctcggcc cgcgtgtact ggcccgtccg cgcggcgagt tccgccagca
     8701 tgcgcagggc ggccacgtcc tgcggcacgc gggcaagatg cgcgacgagc accggctccg
     8761 cgtcggcgta tcgaccctcc gccagtgcgt ctgcggcgcc ggcgaggcgg ccgtcctgcg
     8821 cagccgcgtc gatgagtcga agatatgcgg aatcggctcc cgcgcggtcg cccagcgcat
     8881 acaggtggaa ggccacggcc cgccacgcgt cggccatgtc gggacgaatc tcgagggcgc
     8941 ggcgcagttc cgccaccgcg tcggcgctgc ggccggcgag gccgtaggcg agccccagtt
     9001 cgtagcgtgc ggcgacccag tccggtcgcg ctgcggccag gggctccagg acggccagcg
     9061 cggacggcgc gtcgccgcgc gaccggtgtg ccgcgccgag cacgagccgg gcggccgcgt
     9121 ggtccggtgc ctgcttgaga atttcaccgg cctgctcggc cgcccgctcc gggtccgacg
     9181 cgaccaggcg ggcagcgccc tcgatggcgc tgcggagggc atcgacggcg ttgctcgctg
     9241 tgctcatcgg gcggtcggtc tcgcgaaaga tcgtgcatga gagaccgggt ggcaccgaat
     9301 gtcaattgcc gcgactggcg gcggcaatca ccgtgagcaa aaaaaaacgg cgggccgagg
     9361 cccgccgttc gagttgccgc gtttccgcag cgtgtttctt agaacttcgc agtcagaccg
     9421 acgaacatgt aacgacccag ggcgtcgtac acgcccgggt aggtgttgcc gttcgccgga
     9481 gaggtcggca gcgagcccga cagcggcgga tcctgatcga gcaggttgtt gatgcccgca
     9541 ttgatcgtgt agtgctccgc gaaggtgtag gtcgcgccca ggtcgatgta gttctgggag
     9601 ccgagccact tgtcgaacgg cttctgcgtg tcgtacgaac cctggaggtc cgggtccttg
     9661 ctgtacgtct cgagttcgac cgagtcgatg tagcgccacg ccacggagat gtccacgttc
     9721 cacggcgtcg accagacggt ctggaacttg ctcttccact ccggggtcgg ggtcgtgcac
     9781 ttggacgggc cgtagaagcc cttgcagtcg tacttgccgt agcccgggat ttcctggatc
     9841 tcgaacgagt cgacccaggt ggccagctcg ttgaagttca ggctgcccca gtcaccgatc
     9901 ggcaggttgt agttgaggtt aaagtccgtg ccgctcgtgt ggtagaagcc gaggttgctc
     9961 gtgagtgccg tcacgtagcc gtccttggtc gtccacagcg aaccgcccgc attgcggtgg
    10021 atgttgtcgc agaagaacgg atcggcgaac gcgatgcagt tcgcgacggt cgttgccggg
    10081 gcgatgctgc tgatcacgtc ctcgatcttg atgttccagt agtcgatcga cccggtgaag
    10141 ccctcgagga acgtcggggt cagcacgaga ccgaccgtcc acgtgttgcc cgtctcgggc
    10201 ttcaaggtcg actggccacc gaacagtgcg ttgtactggc cggccgggct taccggcgtg
    10261 gtgccgtacc aggccgccgt gttgccgttg tcgttgtcgg tcagtgcgca ctcggccggc
    10321 gacgcagacg ggttcgaacc cgcacacgga tcgcccgccc agtcgaacag gcccaccgcc
    10381 tgcggcgtga acagctcgag gacgttcgga gcgcgaacgg cgtgctggta gctgccgcgg
    10441 agcttgaagt cgtccatcgg cgcccagtcg agacccaggc cgtacgtgtt ggtattcacg
    10501 ccggtgctgt agtccgagta ccggtagctg ccgttgaacg tcaggctctt cgcgcccggg
    10561 gcatcctcga cgatcgggac gcgggcttcc atgaagacgt ccgtcacgtt gtagccgccc
    10621 ttgacgggca gcagtacgcc ggagccgccg ccgaggtcgc ccgtctggtt ctcggtgtca
    10681 tagtcgagct gcaggtcttc ctggcggtac tcgacgccga acgccacgcc gatgccgctg
    10741 gcagcccacg gcatcttcac gccgtaatcg ccgaggtcgg ccgacagcga gccgttgacc
    10801 accgtctggg ccgtgctgcc gctggagaag ccctggacgt tgatgtagtt gagggccgct
    10861 gcattcacac ccgtcgtggc ggtatttgca ctccagatgt cccacggcac gcaaccggcc
    10921 gcgtacccgg ccaatgcacc cgcacaggcg gccggattgt tgctgaccgc gtcaaagggc
    10981 aggttcgggt cgtagtccgg attggcgacg gcattgagcg cggccagcgc ccgctggttc
    11041 gagacgtagt tgttgacgtg ctcgttcagg atggtcgtgc cgtactggcc gaacaggtcg
    11101 tagttccagt gatcccagat cttgccgcgg atgcccagca cgccctggta gtcggtgtga
    11161 cgcaggtcgt cctggcgcgg gccgccttcg acgttgcggc gctggatgaa caccggggcc
    11221 gtgccagtgg tgatcggggt ggcgttgttg tagcagaggt agttgagcca ctgctggctg
    11281 accagcgggt tggcattggc ggccgtcgga tcgcagggca ggtcgtagta cgtgctgaag
    11341 atgcccgacg gggcgatctg cgcgaccgtg tggtcgtcca tgaacatgaa ctgaccgtac
    11401 gcctggatct ggtcggtcac gtcgtagtgc atgaacacgt ccgcgttcca gcgctcgtcc
    11461 gggcgctggt agtagttcag cggcgcgtag ttgtacagcg agctatcgcg ggccgggccg
    11521 gtcggggtga cgccataagg gcccgtgaag ttgttgcccg cggcatagaa cgagccgttg
    11581 gcacgggtgc ccgaaccacc gcagcgcacg gccttcttct tgttccagct gccgccgatc
    11641 ggggcgccgc cgtagaagta ggccgacgac gccgtgatgc tgtagtaagc gaggatctcg
    11701 gggtccgtct cgatgtacgc ggtctgcagc gcgcaggcac tgctgatgcg gtcgccctgc
    11761 aggaccggat cggtgtggcg gtagcccacg aacatcgtgg cgttgccctt accgtcggcg
    11821 aagttcgagc ccatcatcag gctcaggtcg taggtgtcgc cgtccgttac cgagttgcct
    11881 ggcttggcga atttccgctt ggcgaggatg ccatccatca ggttggcgtt gtcgccgtcg
    11941 ctgttgttcg tgctgtacag gctgtactgg ccgccgagct cgatgccctc gaagtcgtcc
    12001 ttcatgatga agttgacgac gcccgagata gcgtcggagc cgtagaccgc gccggcgccg
    12061 cccgtcagca cttcgacgcg attgatcagc ggggccggga tgaagttgat gtcgggagcg
    12121 atgccggaaa tgctcgccgg gtcacctgcc gggaggcggc ggccgttcac cagcacgagg
    12181 gtgcggctgc tgccgaggtt gcgcaggtcg atggtcgacg tacccgacga accgttggac
    12241 accgtgctgg actgcgcggc aaacgcctgc ggcagctggt tgaccaggtt gacgctgtca
    12301 gtgatgccct gcagttcgac gttctgcgaa ccgatctgcg tgaccgggct ggcaccttcg
    12361 aggttcggct gcggaatgcg cgaaccggtg acgatgatct gctcgacttc gtcaggcgtg
    12421 gcgcccgcct cctgagcgag tgcacccgct gaatacgcgc ccgcgctgac tgcgctggcg
    12481 agcagggcaa cccgcactgc gcgctctata ttgcgattgg ccatcagttg actcccaaga
    12541 atttctagtt gcgaaagacc cacgcccggc tagggcccga acggtggact ggcacgtgcc
    12601 ccgtgcagga accgcgcacc aacagcactc ttggcgacgg cccggcacaa gtggggccga
    12661 tcataccaac ggccaaaggc gtctgtgaag cacctgttgc ggaaattcaa catgccccgg
    12721 ccgggctggc cgtctgttcg atgccgcgct gcagcgtccg gcgaggctgt caaagttgca
    12781 agaccaagtc cagctcggcc ggacctgtgt ctttcaggca acaatgacgg cttagactgg
    12841 tgtcgcggcg atacgacggc cgggatggcc ggccgtgcgg tcagtcctcg gcggcgtagt
    12901 aggtccgctg gccgttcagc aggccgttga ccagttcctt ttccacgtcg gtcaggaacg
    12961 gattccacac gtcgagcatg agctggaccc tgagctggtc gctgtcgttg cgagcctcgt
    13021 ggtctacgct gtcgtcgaac acccaggcct tgccgtactg ccactcccgg gtgtcgttgc
    13081 ccacccggaa cgtgcagccc ggcggcagga tgagtggcag gtgcacgatc agccgaacgt
    13141 tggtgtcccc gttgtgcggc gggatggtcg tgtgggcctc gagcgccgag aacagcacgt
    13201 tgggggagaa gtttggggtc cgcgccaggg gcagcgattc gaccacttcg gccgtgcgcg
    13261 ggcagcgctc gcagtgtgcg tcgatccgct ggccgtgctg ccacagcagg taggtggccc
    13321 agcgcggcga gtgattgaga tccttccact ggtcgagcgg cgcgccgggg gggcgctgga
    13381 tgtaagggtc gaattcgtcg cgctgctcgg cgacgaccgc ctcgcattcc cgacggatat
    13441 cctcggtggc tgcctcgagt tccgggatcc acgggaacag ctcgttcggg tagaactgca
    13501 gcggcggcaa ctgggcgaaa tacagcatca ccggctcggg cgagtagcgt ttcttggcgc
    13561 cggtcatgac cgcgaccgtc tcctcgaagc ggtccaggcg cgcagtcgag tgccgccggc
    13621 gcaggtccgc cagcacgccg tccaggtggc gttcgagcga cccgcggttc tcgtcgacca
    13681 ccgcctgggc gtgctcgacg gccctggcga atgcgggcgc ggcctggtcc ttcgccggca
    13741 tgatcttgag gacgtccttg tacacgcgcg ccgcctgacg gcgcttgccg agccgctcga
    13801 gcagcgtcgc cttgtgcagc agcgccggga agaagtacgg gtcgatggcc agcgcacgcg
    13861 tcacggcggc catttcttcc tggggattcc cgaggtcccg gaaggcgacg gcctggtaga
    13921 tctggaccat cgggtcccgc ggcgccgtgg ccgcggcgtg ctggagcatc tgcagcgcga
    13981 gggtcgcgtc gcggcgcgcc agcgcctgcg tggccagcgc cagcagcgcc tcggtgtggt
    14041 tgggatcggc gccgaggacc cgctgccaga ccagcgccgc ttctgcggca cgtccgacgc
    14101 gggccagttc acgggcctgt tgggccatgg ccgggatccc ggcattgacg cttgcgttca
    14161 tgggccgcga ttgtttcaca aaggtcgccg gggcgacgag ggcagcggct cgaatgctcc
    14221 tgcgtcgaca ggagcagcct ctccggggca agcgacttgc ttcagcggcg aatcaggggt
    14281 cgctcgtgca ggcgcggtcg ctcgtcggcc agcctgcctg gggccgccgg acccggtcgc
    14341 cgaggccgat gccgagcgcg ctggcggcgc cacccttcag ttccacgacc agcgcaaccg
    14401 gtccgtccgc ggcaatcgtc gcgagcgact gcggttgcgc gtcgtgcttg accttgatca
    14461 cgcagccgtc ggcgttcacg aacagcatgt ccagcggaat caacgtgttc ttcatccaca
    14521 tcgccacgta gcgcggcggg tcgtagacga agatcatcgc ctggccatcg cgcatctcgc
    14581 gcacgaacat cagtccctgc tcgtgggtcg cgggtgtgtc ggcgcgccac gcgtcgaagg
    14641 tgcgacgccg ggcgctgtgc gtctccacga cgagcggctc gcgcggaaac tgcgcgagtg
    14701 gaatcaggtc gtcctgcgcg acgtcggcgc gagcggcagc gggaacgagg caccatgcga
    14761 agggcagggc gatcgatgcc gcccgggcca ggagcgaggc gaggagccgt ggcgtcggct
    14821 ggctcactgg aaatccacga ccgcgctcag gtcgaagcgt gagacgacga ccactccttc
    14881 gccgccgacc gcgcgcgccg gctgcgtgca gtatccgcgg cccgtgatcc ggtaggtgtg
    14941 accgttgccg aacaccggca cctgctgcac ttcgtccaca ctgcacttgt cgtcgccctg
    15001 cgtggcgaag aagcgccccg agccctcgcg cacgagcgtg atgttcgcgg gcacgctggt
    15061 tgcattttca ccggcgcgca acggacccat cccgatgacc acgagcagcg gcccctggcc
    15121 cggaatctct cctttgtaga ggaggcgcac gcctacgccg tccgggcgca cgccaccgcg
    15181 acactggggc atcgggtagg cccagcggat ctcggcgtcg attgccccgt gcaggcgcgc
    15241 ctcgagcgag ccttcgttcg cggggatgca cagggcctgc tggcccgacg gcatgctggc
    15301 gccggacgcc gcgcccggtg ttcgtgcaac ctcgctcgtc tcgccgccgg acgcgggacg
    15361 cggcgtgacg tcgggctggc cgctcacgtg cgcggtgccg gcggagtccg cgctccggtc
    15421 gtcgtgggag tgcccgcagc cggtgacgag cagggccagt gcgaggcttg ccaccacgcg
    15481 tttcgtcagg ccagggtcag tcgacccccg gggggcgtac gagcaggttc ttcgcaagca
    15541 ggccatgcag gtagccgata ccccgggcga gatgcagcgt gacggtgagg agcaggactc
    15601 cgacgatcga gatcaacggc agcagcgccg ggtggggcga gtgcacgttc acgtcgccgt
    15661 cgatcgtgat cacgcccgca tggtaaagga gcacggcgat cggcgcgacg gtcgtcgcga
    15721 ccgacaccgc gacgccgacg accacgaacg tgaagtacgc gatgccgagc ggcagcatca
    15781 gcaggaaata caccagcgtg ccccaggtgc gcgggtcctt gagcatgtcg ccgatgcgcc
    15841 tgaggaacgg tgtctcgcgg tcggggtagg gcggccgcct gggcatgcgc gtgccgagca
    15901 tggcctcgac cagccggccc tccgccagcg ccacgatgcg cgtggtgccg acgaacagca
    15961 ggaagaacgg cacgccgatg atcaggatgg ccagtcccac cgacagcgac aggccggtca
    16021 cggcgaaagt gaaatagaag atgccggtca cgagggtgag cagcatgtag agcagcgaca
    16081 ggtaggcgcg cgggtcgaca tagacgccga agaatcggcc agcggtggac tggtgcgcac
    16141 ggggagctgg cgtgcgcagc gccttctgga tcgtcgcctc gttgctgcga tacgcgtccg
    16201 cgacttcgtc gggcgcgccg tagctggaca cgatcgactc gagcacgatg gcctcgtcct
    16261 ggccggctcg cgctgcgagt tccgcgcgca ggtgttcctc ggcatcgtag agcgcgtcct
    16321 gcacgagcgc agggtcggcg cccttcaggg ccgcgcgcag cgcgtcgagg tactgctcga
    16381 ccgttcgcgg tgtctgggcc gtcatggcgt cgcctccccg gcagtggttg tcttgactat
    16441 ggagtccacg aagctgcgcg tctcggtcca ggtcgcgcac caggatttca gcacgtcccg
    16501 gccgagcggc gtgatcctgt aatagcgtcg gggcggcccg gcgtacgagg gcacgacgcg
    16561 gctcgagagc aggcccgccg cactcaggtt gcgcaggacc gggtagagcg ctccctgctt
    16621 gaacgggctc gcgggtgccg cgtcgcccga caggcgcttg gcgatctcgt acccgtatag
    16681 gtccccctcc gtctccgcga gaactgacag caggacgagg gcggtcagcc ccgccgtcag
    16741 ttccttctgg aacttgcgga tcagcggctc cgcgtcgccc acgggcgcgt cgtcaaccgg
    16801 gtcgatggcc acggaattac tcgaggatta gatgcgatgc cttgtattaa acacaaaaca
    16861 gtattacgaa gtcaacaata ttgcgacatt aacatcatct cagagccggg tcccgtgcca
    16921 tcggacgtcc tggcgggtga gaccggaatt ggggcctgac cccatttcga acgggtgccc
    16981 agcgcgatcc gggcgtcggc cgctccatgc cggcggccgg atcgctggca cggcacacgc
    17041 aggtactagt ggagttggca tcgtggattt cgctcacgtg taagacgagg ccgcgtgtct
    17101 gtaacatcga tttcaccgga gccggatgga attgcgcgcc cgatgtccac cgaacagcga
    17161 cagtttgccc cacggcgata cctgagccgc ctgacccgag gcacgcttgc cgtgatcatg
    17221 gcgggcgggc gcggcgagcg cctcaagcaa ttgaccgagg atcggtgcaa gcccgccacg
    17281 ccgttcggcg gcaagttccg gatcatcgac ttcgtgctgt cgaactgcgt gaattccggc
    17341 atccgccaga tctcggtgct cactcaatac aaggcgcact cgctcatcca gcacatcgag
    17401 cgcggctgga gctacctgcg cggcgagttc ggcgagttcg tcgagatcat tcccgcgcag
    17461 cagcggcacg gccttgcgtg gtaccagggc acggccgact cgctctggca gaacatggac
    17521 atcgtcaagt cgcaccggcc gctgcacgtg ctcgtgctcg cgggcgacca catctacaag
    17581 atggactacg gcccgatgat cggcctgcac gtggagaacg aggccgacat cacggtgggc
    17641 gtggtggagg tgccggtcga gagggcgcgc gaattcggcg tgctgacggt cgacgagtcg
    17701 aaccgggtgc tgcgcttcca ggagaagccg ctcgatccgg agccggtgcc gggccgcccc
    17761 gacgtcgcgc tcgcctcgat gggcatctac gtgttcaacc cgaagctgct cgagcgcctg
    17821 ctgcgcgcgg atgcggacga cccgaattcg gcgcacgact tcgggcggaa catcgtcccg
    17881 ggcgccatcg acaagctcag ggtgttcgcc tatcccttcg aggacatccg caccaaggca
    17941 cagaactact ggcgcgacgt cgggacggtg gacgcgtact acgacgcgaa catggagctc
    18001 gtgcgtgtgg cgccggagct gaacatctac gacgagcagt ggccgatctg gacctaccag
    18061 gaacagttgc cgccggcgaa gttcgtgttc gacgaccccg accggcgcgg ctgcgcgatc
    18121 gattcgatgg tgtccggcgg ctgcatcatc tccgggtcgc acgtgtcgaa ctcgctgctg
    18181 ttctcgaacg tgcgcgtcca cgactggtcg tcgatcgacc ggtcggtcgt cctgccgcgg
    18241 gtgcgggtgg gcgagcgctg cgtgatccgg ggcgcgatca tcgatgaagg cgccatcatc
    18301 ccggacggta cgcacatcgg ggtggatccg gtcgccgacg cggagcgcta ttacgtgacg
    18361 gagggcggcg tcgcgctcgt gacttccgct atgctccgtg gcgcgtcgaa agccggctga
    18421 tgccggttcc cgtgccctgg tcctggagga tgccgatgcc ctggatcccg cccaagcgcg
    18481 cgctgccgct cgccgtcgtg ctggcgatgc tcgcggcttg ctcgacgctg acccccaagc
    18541 tcgaaacacc gacggtcagc ctgctcggcg tgcagatgac gagcggcgac atgttctcgc
    18601 agcgtttcaa cgtgcgcgtc aagatcgaga acccgaacga cctcgaggtg ccggtcaaga
    18661 gcctcgagta cacgatctac ctgatggggg accgcttcgg cgaggggacg agcgagcggc
    18721 cgttcgtgct gccggcgcgc ggcgaggcgg agttcgacat gctggtcacg acggacttcg
    18781 tgagcgcgct cgggcggctg gtgagcagga tgggcggcaa caagctcgag aacgtcgagt
    18841 acgctgtcac cggcaagctg cacctcgaca agggcttcgt gcgcacgatc cccttcgaac
    18901 acaagggcct gctcaacttc acgaaggccc cgaagaatta acaggggaca ttctcctatt
    18961 tcgattcgaa ataggagaat gtcccctatt tcttcaggcg ggcgtagagt cgctcgtact
    19021 cggcgccctg ggtgtcccag gagaagtcct ggcgcatgcc gttctgcacc agcctgcgcc
    19081 agaccgtcgg ccacccgtac cactcgagcg cggtctcgag tgcccagcgc acgccgctcg
    19141 catcgaagtc gttgaacacg atgccggtgc cctgtccgct cgcggggtcg aagtgctgca
    19201 cggaatccgc gaggccgccg gtccggcgca cgaccgggac ggtgccgtac ttcaggctgt
    19261 acatctggtt caagccgcac ggctcgtaca tcgagggcat gaggaagatg tcggccgagg
    19321 cctcgatcat gtgcgcgagt tcctcgctgt agccgctgta gaacaccgcc cggcccggga
    19381 aactctgctg cagtccgtgc aggaattcct cgtactgatg atcgccgttg ccgagcgcga
    19441 cgaaccgcag gttgcgggtc cggagcagtt ccggcaacac gtcgaacagc aggtcgatgc
    19501 ctttctgttg cgtgagccgc gtcaccatgc cgacgagcgg cgtgtcgggc gtcgcgggca
    19561 tgcgcagcag gtcgagcagc gcctgcttgt tccagagctt gcccgtgagg tccgtggcgt
    19621 cgtaccggtg cgggatgtag cggtcggtgg acgggctcca ttcgtcgtag tcgacgccgt
    19681 tcaggatgcc ggtcagcgag tcgccgcgcg cccgcaggtc cgggtcgagc ccgtagccgc
    19741 ccgcgggcgt acagatctcg gccgcgtacg tcgggctcac cgtcgtcacc gcgtccgcgt
    19801 gtatcactcc gtggcgcatc gggttgatgt ggcccgccgc gaggtccggc tggtggagcg
    19861 ccgcgatagg cacgccgggt ccgagatccg cggcgtggct cgcggcgaac acgccctggt
    19921 agccgatgtt gtggatcgtg aacacgctgc gggtcgcggc gaagagcccg aggtcccagg
    19981 cataggcgtg cttcagcagc agtggtgcga acgcggtgtg ccagtcgttc gagtgcatga
    20041 tctctggcgc gaaacccatg cgctggcagc agtcgaacgc ggcgcgcgtg agcgcgaggt
    20101 accgcacgtg ctcgtcgggg gcatcggagt agatgcgcgg ccggtcgaac agcgcaggac
    20161 agtccacgag gtacacccag agctgcgtgt cggggacccg cgccgtctgc acgtcgtagc
    20221 ggtactcgtg cggtccgaca cgcagcgtca ggtcgcgcag gaagtccacc ggatggcggt
    20281 cgatgcccgc gagttccatc tggccgtgca gcggcacgaa gacccgcagg tcgtgaccgc
    20341 ggcgcgccag gtagcgcgtc agcgcggcgg cgacgtcgcc gagcccgccg gtcttggcga
    20401 acggcgaata ctccgcggca atgaaacaga tgcgcattcg acaagtgtgc catcagcgac
    20461 gcgcgcgggg cgatcgagcg gtcgtcgcgc ccgctgccgt cgcaggctcg gacggagctg
    20521 gcggggggtc gcgccgccgg ttcaaaatgt cataagtgtc aggcggttac gcgggaccgc
    20581 ccgagcggcg actgcgccgt catgcgggtc tcgcgaccgg cgccgcgccg cgcgatgcga
    20641 agcacgaatc gggtggcgcg cggcggctgc acgactatcc ttgccgccat gaacaccgca
    20701 cccgctgccc cgagcgatcg ccccgtcccg ctcgactatc gccgcatcga gcgcgcaatc
    20761 cgctacatcg accgtcgatt ccgcgaccag ccgagcctgg aggaaatcgc gaagagcgcc
    20821 ggcctcgccc cgcatcactt cgccagggtc ttccgccgct gggccggaat ttcaccgaag
    20881 cagttcgtgc agcacctcac gctcgaggcg gcgcgcgact cgctcggccg cgacgagtcg
    20941 gtgctgcagg cggcgctgga tgcggggttg tcgggcccgg ggcgactgca tgacctgttc
    21001 gtcactctcg aggccgccac gcccggcgat ttcaaggcgc gcgggcgcgg catgacgatc
    21061 cgttacggca ccgcaaccac gccgttcggt gtcgcgacga tcgcgcagac cagccgcgga
    21121 atcggttttg tcggcttcga actggcacgc ggagaagttc caggatggcg tgagttccgc
    21181 gacacctggc gacacgcaga gttcgtcgcc gacgatgcgg ttgcgcagga tctcgcccgg
    21241 aaggtctggg gcgaaggagc gcaagcgggg gagtcgctgc gcctctgggt gcacggttcg
    21301 aatttccaga tccaggtctg gcaggcgctg ctgcgctgcg ctcgcgacac gacggtcaac
    21361 tacgcggacg tcgcgcgcga aatcgggcga cccgaggcgg cccgcgcggt cggcggcgcg
    21421 gtcggcgcca atcccgtcgc atggctcatt ccctgccatc acgtgctgcg cacgagcggc
    21481 gcgctcggcg gctatcgctg gggagtggaa cgcaagcgcg cgatgctggc ctgggagttg
    21541 tcgcgcacgg tcgcggcgca aggctgacgg gtcggaccag caccggtccc ccgagtccag
    21601 ctggccgggg ccgacgatca gccgcgcttg tcgtcctcgt cgcggctctt gtcccgcctg
    21661 ggcaggatcg cgccgagcag gctctcctgg gccttggcga gtgtctcgag attctggcgg
    21721 gtgatgtccg cgaccgtcga gagcggcgtg gaggacacga tcttcgagat ctgcgcctgc
    21781 agtgcttcct gctgattcag gaacgtctcg atgctcttct cgaggaatcc cgcgaggaac
    21841 ccctgcatcg cgttgccgta gaagcggatg atggcctcga gcaccttggt gctcaggatc
    21901 ggccggccga actgctcctg ctcggcgatc acctgcagca ggatgtggcg cgtgatgtcg
    21961 tggccggtct tgtcctccac gaccttgatc ttctcgccct tgacgatcag cgcgcggatg
    22021 tcgtcgagcg tgatgtggcg gctctgcgag gcgtcgtaca gtcgacggtt cgcgtacttc
    22081 ttgatcaggc gttcctggct catccggggt ggtacctcgg tcgtactgcg gcgcacgaca
    22141 tttgctgcac ggctgccggt ggattctatg gagcgttcta ttgcgccgca acagccatgc
    22201 tgcatcgccg cgcgtggtgg tcgccacgta cggcgcgacg tagactcccg gccgttcgct
    22261 gacggaggcg ccgcatgtcg cagcaggaac tgttcgaaag gctgacgagc cagatcggcg
    22321 aggagatcta cgtcggtgaa tggcaccagg tcacccagga gatgatcgac gccttcgccg
    22381 tcgtcaccgg cgaccaccag tggatacacg tggacgtgga acgggcgcgg cgcgagtctc
    22441 catggcgttc gacgattgcc catgggtttc tgacgctgtc cctgctgccg atgctgcgcg
    22501 gcatggggtc cggccagatc ccgtacccgg gggtcaagca gggggtcaac tacggcctgg
    22561 accggctgcg attcccgaat gtggtcaagg ccggcgcgcg cgtccgcgcg agatgcaggc
    22621 tgctcaaggt cgagcagatt ccgggcggcc tgcaggtcac cgagcagttc acggcggaga
    22681 tcgaggggga acagaagccc ggctgcgtgg ccgacaccat cgtcaggctc tatttccaaa
    22741 ccgcctgatc gcccctgatt cccggccggt atgagcgccg ggccgcgact cggcttatag
    22801 tccgggcaaa cgacagccct gccgggcggg agaatgcatc catgatctac gacagcatcc
    22861 tgcagacgat cggccgcacg ccggtcgtgc gcgtcaatcg catcgcgccc gcgcacgtca
    22921 ccatgtacgt gaagtgcgag ttcttcaacc cgctggcttc ggtgaaggac cggctcgcga
    22981 tcgccatcat cgaggacgcc gagcgcagcg gcgcgctcaa gcccggccag acggtcgtcg
    23041 aggccacgtc gggcaacacc ggcatcgcgc tggcgatggt ctgcgccgcg aagggctatc
    23101 cgttcgtgtc ggtgatggtc gagacgttct cgatcgagcg ccgcaagatc atgcgcatgc
    23161 tcggcgccaa ggtgatcctc acgcccgccg cagagcgcgg cagcggcatg gtgcgcaagg
    23221 ctgccgagct cgcgcgcaag cacggctggt tcctcgcgcg gcagttcgag aacgaggcga
    23281 atcccgccta ccaccgccag accacgggac cggaaatcct cagcgatttc gccggccggc
    23341 gcctcgactt cttcgtctcg ggctggggca ccggcggaac gctcacgggc gccggcgaaa
    23401 tgatccgcct cgcgcgcccg gaggtacgca tcgtcgcgac ggagccggcc ggcgccgcgc
    23461 tgctgtcggg ggcggaattc aagccgcaca agatccaggg ctggacgccg gacttcgtgc
    23521 cggccgtgct caatcggtcg gtgccccacg agatcgtcct ggtcaccgac gacgaggcgc
    23581 tcgccgcggc gcgctcgctc gcgagtcgcg agggcatctt ctgcggcatc tcctccggcg
    23641 gcacgttcgc cgcagcactc aaggtcgcgg agaaggcgcc gcaaggctcg gtcatcctcg
    23701 cgatgctgcc cgacaccggc gagcgctaca tgagcacgcc gttgttcgaa ggcattgcgg
    23761 aagggtccga tcccgagccc tgacccgccg ggcgggcatg gccgccaccg tgcccgcccg
    23821 caccctatgc cgtcgacgac cgccagggca tgtcgcgcag cgtggcttgc actgctgctc
    23881 ggtgcctgtt catcccagca gctgtatcac gccggccagg agtggcagcg caccgagtgt
    23941 cgcaagctcg cgccggctga ccaggagcgg tgcctcgcca gcaatgcagt gacgtacgac
    24001 gaatacgagc gcgagcgggc agcggcgggc ggtggaagtt cccggtgatg ccccgtgtcg
    24061 ggcaagtggc cacgcatcgg agtgggccgt cggccgcgcg acttggtggc cgccggtgat
    24121 tacataaggc aatgattctg aagaataaat agatcggccg ctgccgagag ctgcggcccc
    24181 gggaggtggc acgccttgct agaatcgcaa ttcccggcgc gattcccgcc cggagcattt
    24241 cgtcatggct tccaccccgt accagcagct cgagcaggag ttccgccgcc ttcacgcatt
    24301 ccgtggcgcg gcgagcctgt tgcgctggga cgccgcggtg atgatgccgc gcggcagcgc
    24361 ggacg
//
LOCUS       DAOMFD010000012        23496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_363_length_23496_cov_30.936180, whole genome shotgun sequence.
ACCESSION   DAOMFD010000012 DAOMFD010000000
VERSION     DAOMFD010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 23496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_363_length_23496_cov_30.936180"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="PL152_01485"
     CDS             complement(<1..298)
                     /locus_tag="PL152_01485"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HQR47974.1"
                     /translation="MTESFAQLFEESLASQRIRPGQILTGRVIEVGPDKVVVSAGLKS
                     EAVIDIHQFKNERGEIEVSVGDDVEVALDSVEDGSGETRLSREKAKRARTWERLE"
     gene            complement(400..1083)
                     /gene="cmk"
                     /locus_tag="PL152_01490"
     CDS             complement(400..1083)
                     /gene="cmk"
                     /locus_tag="PL152_01490"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HQR47975.1"
                     /translation="MNVPVIAIDGPSGSGKGTIARHVARRLGWHLLDSGALYRLVAMA
                     GIAHGVEPGDEAGHARVAASLDARFETDTLGNERVILEGRDVTLDLRTEAAGAEASRV
                     AAMPPVRAALLERQRSFASAPGLVADGRDMGTVVFATAPLKVYLTASPEERAARRYKQ
                     LKEKGLTANLADLSQEILDRDRRDTNRPVAPLRPATDAVVIDSTGIPIEAVVDQVLAL
                     AKKRIPGAL"
     gene            complement(1093..2394)
                     /gene="aroA"
                     /locus_tag="PL152_01495"
     CDS             complement(1093..2394)
                     /gene="aroA"
                     /locus_tag="PL152_01495"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HQR47976.1"
                     /translation="MSRYRVSPAAGIAGEVTVPGDKSISHRALMLGALASGTTRVTGF
                     LEGEDCLATLRAVAALGVEVRRPGAGEVIVEGVGLDGFKAPAVPLDMGNAGTAMRLFM
                     GILSSQPFDSVLVGDESLMRRPMERAAAPLRAMGARIETRDGRPPVRISGGARLHGIR
                     YEMPVASAQVKSAVLLAGLRAEGNTVVVEPAVTRDHSERMLQAFGCDVTTQGNVITLR
                     PPGRLQATSIEVPGDFSSAAFFLVAGCIGARAPFVVRGVGVNPTRTGLLEMLGLMGAD
                     LRMVNRRTCGAEPVADIEIRPATLKGIRVPEHLVPLAIDEFPAFFIAAACADGETVVT
                     GAEELRVKESDRIAVMASALGRLGVRHEVLPDGLRIEGGSFGGGTVDSHGDHRVAMSI
                     AVASLRASAPIEILDVANVATSFPGFASLANSVGLRIDEQA"
     gene            complement(2396..3601)
                     /gene="pheA"
                     /locus_tag="PL152_01500"
     CDS             complement(2396..3601)
                     /gene="pheA"
                     /locus_tag="PL152_01500"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HQR47977.1"
                     /translation="MARRPVRSRKPKKAAAPAGSRGVRAVPVRKGPAGLTPAKQALEE
                     IRARIDTIDVRLHELINERARLAQQVGLSKHAAGHTVDFYRPEREAQVLRMAIERNRA
                     ERGPLPDEEVVRLFREIMSACLAQQEPLKVAFLGPEGTFTQQAVLKHFGHSVRALPLT
                     SIEEVFREVESGTADLGVVPIENSSEGTVNNTLDMFLTSPLGVCGEVELRVHQNLMGR
                     MATLEDVKRICAHPQALAQCRGWLAEHLQGVERLPVASNAEGARRARDEEGTAAIASA
                     AAAEVYGLNMLVPEIEDRPDNTTRFLVIGSRAFPPSGQDKTTLLVSAAHTDAPGALFG
                     LLEPLARHRISMTRIESRPSRRRKWDYVFFIDLEGHVQSQPLATALAELKARTSLCRV
                     LGSYPKAVL"
     gene            complement(3604..4770)
                     /locus_tag="PL152_01505"
     CDS             complement(3604..4770)
                     /locus_tag="PL152_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HQR47978.1"
                     /translation="MSYKILTLNNISVRGLERLPRERYEVASEIGHPDAILLRSADMH
                     SMDIPASVLAVARAGAGTNNIPVAKLSRRGVPVFNAPGANANAVKELVLAGLFLAARN
                     ICQAWGFVRALEGDDHAIEAAIEKGKKKFVGHELPSRTLGVVGLGAIGVEVANAALAL
                     GMKVLGYDPQITVQRAWQLSSGVEQALSLDDLFARSDIVTVHVPLADATRGLVNEARI
                     RLMRRGSAVLNFARGPIVDEAAILAALEQGQLATYVCDFPTRAAKDHPRVVALPHLGA
                     STDEAEENCAVMVADTLRDYLENGNIRNSVNFPEAVLPRVGENRIAIANDNVPNMVGQ
                     ISTCLAEAKLNIADLLNKSRGDIAYTLIDTDSRIAPDVLGHLRAIEGVLAARLV"
     gene            complement(4781..5863)
                     /gene="serC"
                     /locus_tag="PL152_01510"
     CDS             complement(4781..5863)
                     /gene="serC"
                     /locus_tag="PL152_01510"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HQR47979.1"
                     /translation="MSRVFNFSAGPAALPAEVLEQLRDEMLDWHGQGMSVMEMSHRGK
                     TFVGIAQRAEADLRELLAIPANYKVLFLQGGATAQFAAIPLNLARADSTVDYVNTGAW
                     SKKAIGEAKRYCKVNVAADAAGDNYSSVPERASWRLTPEAAYVHYTPNETIGGVEFGF
                     IPDVGDTPLIADMSSTVLSRPIDVSRFGVIYAGAQKNIGPAGLVVVIVREDLLGRARP
                     DTPTVFDWTAMAADGSMLNTPATYSWYVAGAVFQWIKRQGGLAAMERLNRAKAERLYS
                     VIDGSGFYRNPVRTDCRSWMNVPFVLPSADLDKPFLEGAKAAGLVSLEGHRSVGGMRA
                     SIYNAMPMAGVEALVAYMQEFQRKKG"
     gene            complement(5944..8559)
                     /gene="gyrA"
                     /locus_tag="PL152_01515"
     CDS             complement(5944..8559)
                     /gene="gyrA"
                     /locus_tag="PL152_01515"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQR47980.1"
                     /translation="MTEIAREILHVSIEDEMKQSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMRELGNEWNKPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQPFSMRYLL
                     VDGQGNFGSVDGDAPAAMRYTEVRMSRLASELLADIDKETVDFVPNYDESESEPSVLP
                     TRVPNLLVNGSSGIAVGMATNIPPHNLCEVIDACVAVIDHPAITIPELMEIVPGPDFP
                     TAGIINGAREIYSAYTTGRGRILVRAKTEVEEIDDSGREAIIVTELPYQVNKARLLER
                     IAELVRGKLIEGISELRDESDKDGMRVVIELKRGEVADVVLNNLYKQTPMESVFGVNM
                     VALQDGQPKLLNLKELIEAFLRHRREVVTRRTVFELRKARERGHILEGLAIALANIDE
                     VIALIKASPSPAEAKAGLMGRAWGPGAVPAMLERAGAIQTRPDGLGEEFGLRGGAYRL
                     SEAQAQAILDLRLHRLTGLEQGKILDEYAELLDLIRDLSDILARPERLLAVIRTELLE
                     IREQYGDQRRTRIEADESDLTIEDLIAPQDVVVTLSHGGYAKAQPVSEYRAQGRGGRG
                     KSATSVKDEDFVDKLFVANTHDTLLCFSSHGQLYWLKVYRLPQAGRGSRGKPIVNLLP
                     LEEGERITAVLPIREYESDRYVFMATAQGTVKKTPLAAFSRPRAAGIIAVGLEAGDKL
                     VGVAITDGTRDVILCSSGGKAIRFAEDDVRPMGRTAAGVRGIRVPEGEVVISLIIPDK
                     DGLILTASENGYGKLTSEAEFPVHGRGGQGVIAMQTSERNGRLVAATQIATTDELMLM
                     SSSGTLVRTRAEEISVLGRNTQGVRLIRLDEGERLIGVEPVEPETEEDAGEADAGDPG
                     VVDSEPDAPGSPAIH"
     gene            8715..10055
                     /locus_tag="PL152_01520"
     CDS             8715..10055
                     /locus_tag="PL152_01520"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HQR47981.1"
                     /translation="MESIDTLVTARWVLPVEPDGRVLDDHALAIRGGRIVDLLPADDA
                     AARYSAREVIDRPTHVLMPGFVNAHTHAAMTLLRGRAENLRLGPWLRDGIWPLERRWA
                     DPEYVRDGTELAIAEMLRAGVTCFGDMHLWPDVAAQTAAECHMRASIGLVVTEAATGW
                     AATADEYIDRGMQLRDQYRGDPLVSTHFAPHAPYSVSDATLARVRRLADELDLPVAMH
                     LHETAWEIEHSERKFGRRPLSRLASLGLASPQLVAVHMAHVVPQDLDTLAGCGASVVH
                     CPESNLKLGAGTCPLPDLLGRGVRVAIGTDGAASNNDLDVLAEARAAGLLAAGITGVP
                     GTLIAADLLRMATLEGARALGLGEVTGSLATGKWADLCCLDLSRPRSWPVHDVAATVV
                     YSASSCQVTDTWVAGRRLLADDALLYLDEHEVLDRAENWRRRFDSPAMESAADG"
     gene            10048..10773
                     /gene="ubiG"
                     /locus_tag="PL152_01525"
     CDS             10048..10773
                     /gene="ubiG"
                     /locus_tag="PL152_01525"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017393471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HQR47982.1"
                     /translation="MAETANVDRAEIARFQASASRWWDPEGEMRPLHDLNPVRLGYVE
                     RGGPLAGRNVVDVGCGGGLLAEAMARQGARVTGLDMASELLQVAALHAMESGITVDYR
                     LESAEQHAAAHPGRYDIVTCMEMLEHVPDPRSVIAALASLARPGGHVFVSTINRTPRA
                     YLHAVVGAEYVLRLLPPGTHTYDKFIRPSELAAWGKMHGLQTVDVAGLDYDPFARKAR
                     LSSDARINYVMHLMKPESAAARE"
     gene            10773..11459
                     /gene="gph"
                     /locus_tag="PL152_01530"
     CDS             10773..11459
                     /gene="gph"
                     /locus_tag="PL152_01530"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01449.2"
                     /note="PGP is an essential enzyme in the glycolate salvage
                     pathway in higher organisms (photorespiration in plants).
                     Phosphoglycolate results from the oxidase activity of
                     RubisCO in the Calvin cycle when concentrations of carbon
                     dioxide are low relative to oxygen. This enzyme is a
                     member of the Haloacid Dehalogenase (HAD) superfamily of
                     aspartate-nucleophile hydrolase enzymes (PF00702); Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0008967 - phosphoglycolate phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA];
                     GO_process: GO:0009853 - photorespiration [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HQR47983.1"
                     /translation="MRSHAALLLDLDGTLLDTAPDMGGALNRLRSENGLDPLPHETIR
                     PVVSHGSARLVSLGFPQAVGDEYERLRQRFLELYASNIAEHTSLFPGAESLLAELERL
                     AIPWGVVTNKPGWLTDPLMQELGLFDRAACIVSGDTVAERKPHPLPLLHASRLLGVPP
                     VQCVYAGDAERDIQAGRAAGMTTVVAAYGYIGSDEDPLAWQPAGIVTRPEHLLDWLGT
                     DPARAVAGRS"
     gene            11456..12724
                     /locus_tag="PL152_01535"
     CDS             11456..12724
                     /locus_tag="PL152_01535"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HQR47984.1"
                     /translation="MTGGATLWVILVGLLAFGVGWLIGRRRSHALELELAAARAEVRS
                     AADVAREREQALELALTRLRTGFDTVAGEALRGNSETFLQLARQVLGQQHELATRSLA
                     EREKAVETMLAPVRDALQRTHEQIARIEKERAETFGALRSSIEGVALGQQALQRETRN
                     LVTALRRPEVRGQWGEMTLRRLAELAGMIEHCDFAEQVTVQGEDGSLRPDMIVNMPDG
                     RQLVVDVKTPLDAYLSAVEATTDEDRAAAIRRHAQAVVERVRQLASKSYWTQFEHSPD
                     FVVLFIPGDQFVSAAIGEVPTLLEDAIRQHVIIATPTSFIALLKAVAYGWRQNALAEN
                     AAHIQELAEELYKRLATFGEHLARIGRSLGQSVDAYNSAVGSLERQVLPGARKFTELG
                     LRPGKEIGDIAPVDKLARVPKSGDGDGADE"
     gene            12717..13472
                     /locus_tag="PL152_01540"
     CDS             12717..13472
                     /locus_tag="PL152_01540"
                     /inference="COORDINATES: protein motif:HMM:NF006509.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HQR47985.1"
                     /translation="MSEVDPRAYVPPPGALQDRVILVTGASGGLGSELARTCAVLGAR
                     VVLTGRHVKRLETVYDQVRAAGGPRPSIVPLDFERADASHYSALVDAVAAEYGRLDGL
                     AHVAGLLGDRSPIEHYDVVTWMRVMHVNVNAAFILTQALLPLLRVSQDASVVFTTSGV
                     SQRARAYWGAYAVSKFALEGLMQVLADETDTSTHIRVNSVNPGRIRTSMRAKAYPGED
                     AASVPLPSQALPTYLYLLGPASRGITGRRFDAQ"
     gene            complement(13444..14247)
                     /locus_tag="PL152_01545"
     CDS             complement(13444..14247)
                     /locus_tag="PL152_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQR47986.1"
                     /translation="MDERLHKLLAQHGLGSRRQVESWIGEGRVLVNGRPAEIGQKVRP
                     GDRVVVDGRDVTKRLASTPSQRVIVYHKPSGEMLRRQAGDERAGVEMRLPSLRAGRWL
                     PVNALGFGEDGLLIMASDGTLVSAITRRAAAIPVEYRVRALRPRQADDWPALPLETEV
                     EGQHVAFSAVERLEGGLTNAWFKVTAERTLPRGAVRALFDAAGLKVSRTMLVRWGPVS
                     LPRDLPRGRSRELVGTELEALLGLAGRQAEARPAARPRRRPTGRRSGGR"
     gene            complement(14257..15012)
                     /gene="scpB"
                     /locus_tag="PL152_01550"
     CDS             complement(14257..15012)
                     /gene="scpB"
                     /locus_tag="PL152_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HQR47987.1"
                     /translation="MSDHPLCNVVEAVLLAAGRPVTVDQVLELFDEHERPAAEDVLEA
                     LAELGSRLEGTGVELREVASGWRVQVRPQYSATVSRLWQERPSRYSRALLETLALIAY
                     RQPITRGEIEDIRGVTVASTIMRTMQERNWIRVVGHREVPGRPELLGTTREFLDYFGL
                     RSLDDLPTLAELQDLGDIKVQLELPGGADGEEMPTMPEALSGKVLATAGSMVADEASD
                     DEAAPDDELEAGRAGPEDEAPTLVALPPGDDQR"
     gene            complement(15075..15842)
                     /locus_tag="PL152_01555"
                     /pseudo
     CDS             complement(15075..15842)
                     /locus_tag="PL152_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007895030.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
     gene            complement(15938..17143)
                     /locus_tag="PL152_01560"
     CDS             complement(15938..17143)
                     /locus_tag="PL152_01560"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF008922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HQR47988.1"
                     /translation="MTTSRRVLSGMRPTGALHLGNYFGALRNWTELQYEYDCYFFIAD
                     YHALTTGYDETAGIESNAWQIVIDWLAAGLNPSVATLFIQSKVPEHAELHLLLSMITP
                     LGWLERVPTYKDQQEQLKEKDLATYGFLGYPLLQSADILLYRPMYIPVGEDQVAHVEI
                     TREIARRFNFIYGRETDFEAKAERAVKNLGAKNAALYRDLRKRYQERGEEEALGKARA
                     LLEGNSRLTLADRERLLGYLEGTGRTILPEPEALLTPTPKVLGLDGRKMSKSYGNTIG
                     MREDPDAVAAKLKTMQTDPARVRRTDPGDPEKCPVWDLHKLYSSDETRRWVDQGCRTA
                     GIGCLECKKPLIERVVAEVSALRQRGQEFEENPDLVRGILAEGCEKARAVAGQTLDDV
                     KQAMGLGYR"
     gene            complement(17217..17837)
                     /locus_tag="PL152_01565"
     CDS             complement(17217..17837)
                     /locus_tag="PL152_01565"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQR47989.1"
                     /translation="MALLLTIHPVSPQPRLIRQAIAAIRDGSVVAYPTDSCYALGCLI
                     GDKAAMERIRRIREADKNHNFTFVCSDLSQIAKYARVDNRQYRTLRAFTPGPYTFLLE
                     ATREVPKRLQNPKKRTIGIRVPDNAIVRMLLAELGEPIMSSTLLLPGEPTPMTDPQEI
                     KARLEHAVDLVIDGGRCGFEPSSVVDLSGEAPVVVRRAKGDVAAFE"
     gene            17933..18478
                     /locus_tag="PL152_01570"
     CDS             17933..18478
                     /locus_tag="PL152_01570"
                     /inference="COORDINATES: protein motif:HMM:NF001325.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HQR47990.1"
                     /translation="MQFVVDLLPVIAFFAAYKFKGIYVATAVLIVGVLLQTAVTWLRF
                     RKVSSMLLGSAALVLLFGGLTLYLHDATFIKWKPTIVDWLFAAVLLYGQFARGPNFLQ
                     RMMGEQVSLVPADWTRLNVAWTVFFLVSGVLNLYVAYNYPESTWVSFKLFGLMGLMLL
                     FAVAQGFWIARRAIVADTEQA"
     gene            18480..18779
                     /locus_tag="PL152_01575"
     CDS             18480..18779
                     /locus_tag="PL152_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HQR47991.1"
                     /translation="MLYVITGRDAPGSLATRRRARPAHLSRIRELSDAGRVVLAGPCP
                     AVDSAEPGEAGFTGSVIVADFPSLDAARAWIAADPYVTDGVFESHEVCPFIKVLP"
     gene            18776..19054
                     /locus_tag="PL152_01580"
     CDS             18776..19054
                     /locus_tag="PL152_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family protein"
                     /protein_id="HQR47992.1"
                     /translation="MTATRLERLTERLNEALAPRQLDVVDDSHHHAGHAGAADGRGHF
                     SVLIVSDRFAGLGTLKRHRLVYDAVGDMMTTDIHALSIQAIAPGEATT"
     gene            19111..19929
                     /locus_tag="PL152_01585"
     CDS             19111..19929
                     /locus_tag="PL152_01585"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR47993.1"
                     /translation="MIAGIALLAGCTKPAGEAAKPAASAASPAGKPAADVATVNGKPI
                     TAEQFSVFVEAVTGKPGTDLPPEQKSQMLDQLINMELASQAAEKEGLQNDAATKARIA
                     LLTMQVLAQAGTEKYLKAHPVSDSEVKAEYDEQVAKMPMEYKARHILVDSKEQAEAII
                     KQLDAGADFAKLAAKDSKDPSGKNGGDLGWFSLQSMVKPFSDAVAALKKGEITQQPVQ
                     TQYGWHVIQLEDTRTPTAPAFDDVKQQVKTLAERKKLQAYLDELRKTAKIQKTG"
     gene            20562..21401
                     /locus_tag="PL152_01590"
     CDS             20562..21401
                     /locus_tag="PL152_01590"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HQR47994.1"
                     /translation="MSENVKKSLLAACRHLLHPLVRILLRHGVGYGEFSDSVRGAYID
                     IASAELMNPNQPQSDARLGILTGIGAQEVHRIREADAAQESEVGLNQIARVLQGWSQD
                     PEYLGPYGLPLEVPFAGDRISFEALVGRYAVGAEPRPLLGELIRVGAAVEADDGYVRL
                     LNRTYLPTPLDPVGLERLGNVVNYFIDTVDFNLQKKRQGEGRFERYAITMEGLSPDSF
                     AAFDQLVREKGQEFLEILDDWLGQYEIKGGHRLPPDASIRTGVGVFHFVDRFPTTTDD
                     SFR"
     gene            21416..>21756
                     /locus_tag="PL152_01595"
     CDS             21416..>21756
                     /locus_tag="PL152_01595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47995.1"
                     /translation="MVDSDTKDKRGLGMSTAGRVQTIALAVGLVLAAGSTSAANRGNA
                     IIGGDLAKAAPSAIIGGDAIIGGDAIIGGDARKARRGNAIIGGDAIIGGDLAKAAPSA
                     IIGGDAIIGGDA"
     assembly_gap    21757..21856
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <21857..22342
                     /locus_tag="PL152_01600"
     CDS             <21857..22342
                     /locus_tag="PL152_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR47996.1"
                     /translation="RRGNAIIGGDLLATYPSNALVVGPVEKVSVATGQVTILGQTFQG
                     PAGSRQLQSMSEQLASGTPVLATITGAPDKSGHLHPISMTANSGPYAAGASRVLVVGR
                     VSSTSLATGHFSVGQLVVDYTSLLADSSVQVASGSTVAIVGVQAAKGGALQALQLRVL
                     R"
     gene            complement(22421..23260)
                     /locus_tag="PL152_01605"
     CDS             complement(22421..23260)
                     /locus_tag="PL152_01605"
                     /inference="COORDINATES: protein motif:HMM:NF039473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6502 family protein"
                     /protein_id="HQR47997.1"
                     /translation="MSAAIQDKVLAATMMCLRPLARLLLRSGINYRQFADAAKIAFLE
                     EAASERDARGRVANVSRMAIRTGLSRKEVARIRDRRTLEKGTVVELVGEIKHAGHAAR
                     VLQLWYSDARFVDSSGEPSELRFAGDGVTFTNLVRLVGGDVPAGAVRAELVAAGSVEE
                     TAGGGLRAVKRYFVPGNVNEDLVIGLTQIVQPVIEGLVRNTGDENVEPFVQRLAYSDR
                     LLPAAVPLFRHIARSRCSDFVQSIDDWLSSNEIAAGAHADESLRVGVGVFYFEGTAPS
                     GAE"
BASE COUNT         3698 a         8063 c         7766 g         3869 t
ORIGIN      
        1 actcgaggcg ctcccaggtg cgggcgcgct tggccttctc gcgcgacagg cgcgtctcgc
       61 cggacccgtc ctcgacgctg tcgagggcca cctcgacgtc gtcgccgacg ctgacttcaa
      121 tctctccacg ctcgttcttg aactggtgga tgtcgatgac ggcctcggac ttgaggcccg
      181 cgctcacgac gaccttgtcg ggaccgactt cgatcacgcg gccggtgagg atctggcccg
      241 ggcggatgcg ctgactcgcc aggctctctt cgaacagttg ggcaaagctc tcggtcattt
      301 gactgcactt ccaggggtct cgcgacccgt atgcccgccg gctccatgcc agcgagggtt
      361 gacgacgaca cgcccgcgtc cattcgggcg acaacaggat caaagggccc cagggatccg
      421 cttcttggcg agggcgagca cctggtcgac caccgcctcg atgggaatgc ccgtcgagtc
      481 gatcacgacc gcatccgtcg cggggcgcag tggcgccacg ggccggttgg tgtcacgccg
      541 atccctgtcc aggatctcct gcgaaaggtc ggcgagatta gcagtaagtc ccttttcttt
      601 caactgctta tagcgcctcg cggcgcgttc ctcggggctg gccgtgaggt agaccttgag
      661 gggcgccgtg gcaaagacga ccgtccccat gtcgcgtccg tcggcaacga gaccaggggc
      721 cgaagcgaac gacctctggc gctccagcag cgcggcccgg accggtggca tggcggcgac
      781 ccgtgacgcc tcggccccag ccgcctccgt gcgcaggtcc agggtgacgt cgcgaccctc
      841 gaggatgacc cgctcgttgc cgagcgtatc ggtctcgaac cgggcgtcga ggctcgcagc
      901 gacccgcgca tggcccgcct cgtcgcccgg ctcgacgccg tgggcaatcc cggccatggc
      961 gaccaggcga tacagggccc cgctgtcgag caggtgccag ccgagcctgc gggccacgtg
     1021 acgcgcgatc gtgcccttgc ccgacccgct cggcccgtcg atggcgatga ccggaacgtt
     1081 cacccgctgg cctcaggcct gctcgtcgat gcgcaggccg acggaattgg cgagcgatgc
     1141 gaagccgggg aaggacgtgg cgacgtttgc gacgtccagg atctcgatcg gcgcggaggc
     1201 acgcaggctc gccacggcaa tcgacatcgc aacccggtgg tccccgtggc tgtcgacggt
     1261 gccgccgccg aagctgccgc cttcgatccg cagcccgtcg ggcagcactt catgccgcac
     1321 gccgagccgg cccaatgcgc tcgccatcac cgcgatacgg tcactctcct tgaccctcag
     1381 ttcctccgcg ccggtgacga ccgtttcacc gtccgcacag gccgcggcga tgaagaatgc
     1441 cggaaactcg tcgatggcga gcggcacgag gtgctccggc acgcggatcc ctttcagggt
     1501 cgccggccgg atctcgatgt cggcgacggg ctccgcgccg caggtgcgac ggttgaccat
     1561 gcgcaggtcg gcgcccatga gcccgagcat ctcgagcagc ccggtgcgtg tcgggttcac
     1621 acccacgccg cggaccacga atggcgcgcg cgcgccgatg caaccagcta ccaggaagaa
     1681 tgcagcagag gaaaaatcgc ccgggacttc gatgctggtc gcctgcaggc gaccgggtgg
     1741 ccgcagcgtg atgacgttgc cctgggtcgt gacgtcgcag ccgaacgcct gcagcatccg
     1801 ctccgaatgg tcgcgggtga ccgccggctc gaccacgacg gtattgcctt cggcacgcaa
     1861 gccggccagc agcaccgcgg acttcacctg tgcgctcgcg acgggcatct cgtagcggat
     1921 gccatgaagc cgcgctccgc cgctgatgcg cacgggcggg cgaccgtccc gggtctcgat
     1981 gcgggccccc atcgcacgga gcggcgcagc agctcgttcc atcgggcggc gcatgagtga
     2041 ctcgtcgccg acgagcaccg aatcgaacgg ctgggaactc aggatgccca tgaacaggcg
     2101 catcgccgtg ccggcattgc ccatgtcgag cggaaccgcc ggcgccttga atccatcgag
     2161 gccgacccct tccacgatga cctcgcccgc tcctggccga cgaacctcga cgcccagagc
     2221 cgcgaccgcg cgcagcgtcg ccaggcagtc ctcgccctcg agaaaaccgg tgacccgcgt
     2281 cgtgcccgac gccagcgcgc cgagcatgag tgcccggtgg gagatcgact tgtcgcccgg
     2341 caccgtgacc tcgccggcaa tgccggccgc gggcgacacc cggtaacggc tcatctcaca
     2401 gcacggcctt cgggtacgaa cccagcaccc ggcacagcga ggtgcgtgcc ttcaactcgg
     2461 caagcgcagt ggcgagcggc tgcgactgca catgcccctc gaggtcgatg aagaacacgt
     2521 agtcccactt gcgccgtcgc gacgggcggg actcgatgcg ggtcatgctg atgcggtggc
     2581 gggccagcgg ttcgagcagg ccgaacagcg cgccgggcgc atcggtgtga gccgccgaca
     2641 ccagcagcgt cgtcttgtcc tgcccgctcg gcgggaacgc ccggctgccg atgaccagga
     2701 atcgcgtggt gttgtccggt cggtcctcga tttccggcac gagcatgttg agcccgtaca
     2761 cctcggcggc ggctgcgctc gcgatggcgg ccgtgccctc ctcgtcccgt gcgcggcgcg
     2821 cgccttccgc gttgctcgcc accggcaggc gctcgacacc ctgcaggtgc tccgcgagcc
     2881 agccccggca ttgggcgagt gcctgcggat gtgcgcagat gcgcttgacg tcctccagcg
     2941 tggccatgcg tcccatcagg ttctggtgga cgcgcagttc gacttcgccg cagacgccga
     3001 gcggcgaggt gaggaacatg tcgagcgtgt tgttcaccgt gccttcgctc gaattctcga
     3061 tcggcacgac gccgaggtcg gccgtccccg actcgacctc gcggaacact tcctcgatgg
     3121 aggtcagcgg cagcgcgcgg accgagtgcc cgaagtgctt gagcaccgcc tgctgggtga
     3181 acgtgccttc cgggccgagg aacgcgacct tgagcggctc ctgctgggcg aggcacgcgg
     3241 acatgatctc gcggaacagg cgcacgacct cctcgtccgg cagcgggccc cgctcggcgc
     3301 gattgcgctc gatcgccatg cgcagcacct gggcctcgcg ctcgggccgg tagaaatcca
     3361 ccgtgtgccc ggccgcgtgc ttggacaggc cgacctgctg agcgagcctg gcgcgctcgt
     3421 tgatcaactc gtgcagcctc acgtcgatcg tgtcgatgcg ggcgcgaatc tcctcgaggg
     3481 cctgcttggc gggcgtcaat ccggccggcc cctttcgaac cgggacggcg cgcacgccgc
     3541 gcgagccagc cggcgccgcc gccttcttcg gcttgcgact gcggactggc cggcgtgcca
     3601 tcgtcagacg agcctcgcgg ccagcacgcc ttcgatggcg cgaaggtgtc ccagtacgtc
     3661 gggcgcgatc cgggaatccg tgtcgatcag ggtgtaggcg atgtcaccgc gcgacttgtt
     3721 cagcaggtcc gcgatgttga gcttcgcctc ggccaggcac gtcgagatct gtcccaccat
     3781 gttcggcacg ttgtcgttgg cgatggcgat gcggttctcg ccgacgcgcg gcagcaccgc
     3841 ctccgggaaa ttgaccgagt tgcggatgtt accgttctcg aggtaatcgc gcagggtgtc
     3901 ggcgaccatg accgcgcagt tctcctccgc ctcgtccgtg gacgcgccga ggtgcgggag
     3961 cgccacgacg cgcgggtggt ccttggcggc gcgcgtcggg aagtcgcaga cgtaggtcgc
     4021 gagctggccc tgctcgagcg ccgcgagtat ggcggcctcg tccacgatcg gcccgcgggc
     4081 gaagttgagc accgcgctgc cgcggcgcat caggcggatg cgcgcctcgt tgaccagccc
     4141 gcgagtcgcg tccgccagcg gcacgtggac cgtgacgatg tcgctgcgcg cgaacaggtc
     4201 gtcgaggctg agcgcctgct cgacgcccga cgacagctgc caggcgcgct gcacggtgat
     4261 ctgcgggtcg tagccgagca ccttcatgcc gagcgccagc gccgcgttcg ccacctcgac
     4321 gccgattgcg cccaggccca cgacgccgag cgtgcgcgaa ggcagctcgt ggccgacgaa
     4381 cttcttcttg cctttctcga tcgccgcctc gatggcgtgg tcgtcgcctt ccagcgcgcg
     4441 cacgaagccc caggcctggc agatgttccg cgcggcgagg aacagtcccg ccagcaccag
     4501 ctccttcacc gcgttcgcgt tggccccggg cgcgttgaat accgggacgc cgcggcggct
     4561 cagcttcgcc accgggatgt tgttcgtgcc ggcgccggcg cgtgccaccg ccaggacgct
     4621 ggcggggatg tccatcgagt gcatgtcggc cgagcgcagc aggatggcgt ccggatggcc
     4681 gatctcggag gcgacctcgt agcgctcgcg gggcagccgc tcgaggcccc ggacggagat
     4741 gttgttcagg gtcaggattt tgtacgacat ggcctcggcc tcatcccttc ttgcgctgga
     4801 actcctgcat gtacgcgacc agcgcctcga cgcctgccat cggcatggcg ttgtagatgc
     4861 tggcgcgcat gccgcccacg gagcggtgtc cctcgagcga cacgagtccc gcggccttcg
     4921 cgccttcgag gaacggcttg tcgaggtccg ccgatggcag cacgaacggg acgttcatcc
     4981 acgaccggca atcggtgcgc accgggttgc ggtagaagcc cgagccgtcg atgacggagt
     5041 acaggcgctc cgccttggcg cggttcaggc gctccattgc agcgaggccg ccctgccgct
     5101 tgatccactg gaagaccgcg ccggcgacgt accaggagta ggtggccggt gtgttgagca
     5161 tcgaaccgtc ggccgccatg gccgtccagt cgaagaccgt cggcgtgtcc ggtctcgccc
     5221 tcccgagcag gtcctcgcgc acgatcacga ccacgaggcc ggccgggccg atgttcttct
     5281 gcgcgccggc gtagatgacg ccgaagcgac tcacgtcgat cgggcgggac aggacggtcg
     5341 aggacatgtc cgcgatgagc ggcgtgtcgc cgacatcagg aatgaatccg aactccacgc
     5401 cgccgatcgt ctcgttcggc gtgtagtgca cgtacgcagc ctctggcgtc aggcgccacg
     5461 acgcccgctc ggggacgctc gagtagttgt ccccggcggc gtcggctgcg acgttgacct
     5521 tgcagtagcg cttcgcctcg ccgatcgcct tcttggacca ggcgccggtg ttgacgtagt
     5581 cgacggtcga atctgccctg gcgaggttca gcgggatcgc agcgaactgc gccgtcgcgc
     5641 caccctgcag gaacaggacc ttgtagttgg ccgggatcgc cagcagctca cgcaggtccg
     5701 cctcggcgcg ctgtgcgatg cccacgaacg tcttgccgcg atgactcatt tccatcacgg
     5761 acatgccctg gccgtgccag tcgagcatct cgtcgcgcag ttgctcgagg acctcggccg
     5821 gcaatgcggc cggaccggcg ctgaagttga agacacggct catgggctgg gactctcccg
     5881 gacgatgttg cagtgcagtg tagcagcgca catcgcctgg cggtcacccc gggcgcacgg
     5941 cgctcagtgg atggcgggcg accccggcgc gtcgggttcc gagtcgacga cccccgggtc
     6001 accggcatcg gcctccccgg cgtcctcttc ggtctccggc tcgacgggct cgacgccgat
     6061 gagccgctca ccctcgtcga ggcggatcag ccggacgccc tgggtattgc ggccgagcac
     6121 cgagatctcc tcggcacgcg tgcgcaccag cgtcccgctc gaactcatga gcatgagctc
     6181 gtcggtggtc gcgatctgcg tggccgcgac cagccggcca ttgcgctcac tcgtctgcat
     6241 ggcgatgacg ccctgccctc cgcgtccgtg caccgggaac tcggcctccg acgtcagctt
     6301 gccgtagccg ttctcggacg cggtcaggat cagcccgtcc ttgtcgggga tgatgagcga
     6361 gatcacgacc tcgccctccg gcacgcggat gccgcgtacg ccggccgcgg tgcggcccat
     6421 ggggcgcacg tcgtcctcgg cgaaacggat cgccttgccg cccgacgagc agaggatcac
     6481 gtcgcgcgtg ccatcggtga tggcgacgcc gaccagcttg tctccagcct cgaggcccac
     6541 cgcgatgatc cccgcggcgc gcggacgcga gaacgccgcg agcggcgtct tcttcacggt
     6601 gccctgcgcg gtggccatga acacgtagcg gtcgctctcg tactcacgga tcggcaacac
     6661 ggcggtgatg cgctcgcctt cctccagcgg caggaggttg acgatcggct tgccgcgcga
     6721 accgcggccg gcctgcggca ggcggtacac cttgagccag tagagctgcc cgtggctcga
     6781 gaagcacagc aacgtatcgt gtgtgttcgc cacgaagagc ttgtcgacga agtcctcgtc
     6841 cttcaccgag gtcgcggact tgccgcgtcc gccgcgcccc tgcgcgcggt actcgctcac
     6901 cggctgcgcc ttggcgtagc cgccgtgcga cagcgtgacg acgacgtcct gcggcgcgat
     6961 gaggtcttcg atcgtcaggt cggactcgtc ggcctcgatg cgggtgcggc gctggtcgcc
     7021 gtactgctcg cggatctcga gcaactccgt ccggatcacc gccagcagcc gctcgggccg
     7081 cgcgaggatg tcgctcaggt cgcggatcag gtcgagcagc tcggcgtact cgtcgagaat
     7141 cttgccctgc tcgagtccgg tgagccggtg caggcggagg tcgaggatcg cctgtgcctg
     7201 cgcttccgac agccggtacg cgccgccgcg aaggccgaac tcctcgccga ggccgtcggg
     7261 ccgcgtctgg atcgccccgg cgcgctcgag catcgccggc accgcgccgg gaccccaggc
     7321 gcgacccatc aggccggctt tcgcttcggc cggcgaaggc gacgccttga tcagcgcgat
     7381 cacctcgtcg atgttggcga gcgcgatcgc gagcccctcg aggatgtggc cgcgttcgcg
     7441 ggccttgcgc aactcgaaca cggtccgccg cgtgacgact tcgcgccggt ggcggaggaa
     7501 tgcctcgatc agttccttca ggttcagcag cttcggctgg ccgtcctgca acgcgaccat
     7561 gttgacgccg aacacggact ccatcggcgt ctgcttgtag aggttgttca ggacgacgtc
     7621 ggcgacctcg ccccgcttga gctcgatcac cacgcgcatg ccgtccttgt cggactcgtc
     7681 acgcagctcg gagatgccct cgatcagctt gccgcgcacc agttcggcga tgcgctcgag
     7741 cagccgcgcc ttgttcacct ggtacggcag ctcggtgacg atgatggctt cgcgcccgct
     7801 gtcgtcgatt tcctcgacct cggtcttcgc gcgcaccagg atgcggccgc ggcccgtcgt
     7861 gtaggcgctg tagatctcgc gcgcgccgtt gatgatgcca gccgttggaa agtccggtcc
     7921 aggaacgatt tccatcagtt ccggaatcgt gatcgcaggg tggtcgatca ccgccacgca
     7981 ggcgtcgatg acttcgcaga gattgtgcgg cggaatgttg gtcgccatgc ccaccgcgat
     8041 gccggacgac ccgttgacca ggaggttcgg tacgcgtgtc ggcagcaccg acggctcgct
     8101 ctcggactcg tcgtagttcg ggacgaagtc cacggtctcc ttgtcgatgt ccgcgagcaa
     8161 ctcggaggcg agccgcgaca tgcgcacctc ggtgtagcgc atcgccgccg gggcgtcgcc
     8221 gtccaccgaa ccgaagttgc cctgaccgtc caccagcagg tagcgcatcg agaacggctg
     8281 cgccatgcgc acgatcgtgt cgtacaccgc cgtgtcgccg tgcgggtggt acttgccgat
     8341 cacgtcgccc accacgcgcg cggacttctt gtacggtttg ttccactcgt tgccgagctc
     8401 gcgcatcgcg tagagtacgc ggcggtgcac gggcttcagt ccgtcccgca cgtcgggcag
     8461 cgcgcggccc acgatcacgc tcatcgcgta atcgaggtac gactgcttca tctcgtcttc
     8521 gatgctgacg tgcaggattt cgcgcgcgat ttcggtcatg tgagcggcgt ggaccagggt
     8581 gccggcgaag ccggtccaaa gacccggaat gataccacaa gggtcgcccc ggaccggcgc
     8641 tgcgggcacg ctgcggacgc gtgccgtacg aggcgcctcg gtatactgcc ggcccccttc
     8701 cctgccaccg cgcgatggaa tcgatcgaca cgctcgtgac cgcacgctgg gtgctgcccg
     8761 tcgagcccga cggacgcgtg ctcgacgacc acgcgctcgc catccgcggc ggccgcatcg
     8821 tcgacctgct gccggcggat gacgccgctg cccgctattc agcccgcgag gtcatcgacc
     8881 gtccgaccca cgtgctcatg cccgggttcg tcaacgccca cacgcacgcg gccatgacgc
     8941 tgctgcgtgg cagggccgag aacctgcggc tcggcccgtg gctgcgcgac ggcatctggc
     9001 cgctcgagcg acgctgggcc gatcccgagt acgtgcgcga cggaacggaa ctggcgatcg
     9061 ccgaaatgct gcgcgccggc gtcacctgct tcggcgacat gcacctgtgg ccagacgtgg
     9121 cggcgcagac cgccgccgaa tgccacatgc gcgcgagcat cgggctggtg gtcaccgagg
     9181 cggccaccgg ctgggctgcg acggccgacg agtacatcga tcgcggcatg caattgcggg
     9241 accagtatcg cggcgacccg ctcgtgtcca cgcacttcgc tccgcatgcg ccctactcgg
     9301 tcagcgacgc gaccctggcg cgggtgcgac gcctggcgga cgagctcgac ctgccggtcg
     9361 cgatgcacct gcacgagacg gcctgggaga tcgagcacag cgaacggaaa ttcggccggc
     9421 ggccgctgtc acgtttggca tcgctcgggc tcgccagccc gcagctcgtc gccgtgcaca
     9481 tggcgcacgt cgtgccgcag gacctcgaca cgctggccgg atgcggcgcg agcgtggtcc
     9541 actgcccgga atccaacctg aagctgggcg ccggcacctg cccgttgccc gacctgctcg
     9601 gtcgcggcgt tcgagtggcg atcgggaccg acggcgccgc atcgaacaac gacctcgacg
     9661 tgctggccga ggcgcgcgcg gcgggattgc tcgccgcagg catcaccggg gtgcctggca
     9721 ccctgatcgc ggcggacctg ctgcgcatgg ccacgctcga gggcgcacgg gcactgggcc
     9781 tcggcgaggt gaccggcagc ctggcgacgg ggaaatgggc agacctctgc tgcctcgacc
     9841 tgtcccggcc ccgcagctgg ccggtgcacg acgtggccgc gaccgtggtg tactcggcct
     9901 cgtcgtgcca ggtcaccgac acgtgggtcg ccgggcgacg actgctcgcg gacgacgcgc
     9961 tgctgtacct cgacgagcac gaggtactcg atcgggccga gaactggcgt cgaaggttcg
    10021 actcgcctgc catggagagt gcggcagatg gctgaaaccg ccaacgtcga tcgtgcggaa
    10081 atcgcgcggt tccaggcctc ggcgagccgc tggtgggacc ccgagggcga gatgcgcccg
    10141 ctgcacgacc tgaatcccgt gcgcctcggg tacgtcgagc gcggcggacc actcgccggc
    10201 cgcaacgtcg tggacgtcgg ctgcggcggc ggcctgctcg ccgaggcgat ggcgagacaa
    10261 ggggcacgcg tcaccggcct cgacatggcc tccgagctgc tgcaggtcgc cgcgctgcat
    10321 gcgatggaat ccggcatcac ggtggactat cgcctcgagt cggcggagca gcacgcggcg
    10381 gcgcatccgg ggcgttatga catcgtcacc tgcatggaaa tgctcgagca cgtacccgac
    10441 ccgcggtcgg tcatcgcagc gctcgcgtcg ctggcgcgcc cgggcggcca cgtgttcgtc
    10501 tcgacgatca accgcacgcc gcgcgcctac ctgcacgccg tggtcggcgc ggagtacgtg
    10561 ctgcgactcc tgccgccggg cacccacacc tacgacaagt tcatccgtcc gtcggagctg
    10621 gccgcctggg gcaagatgca cggcttgcag acggtcgacg tggccgggct cgattacgac
    10681 ccattcgccc gcaaggcacg actgtcgagt gacgcgcgca tcaactacgt catgcacctc
    10741 atgaagcccg aatctgcggc ggcccgcgag tgatgcgcag ccacgccgcc ctgctgctcg
    10801 acctcgacgg cacgttgctc gacacggcgc cggacatggg tggcgcgctg aatcgactcc
    10861 gcagcgaaaa cgggctcgat ccgctccccc acgagaccat ccgcccggtg gtctcgcacg
    10921 gttcggcccg actcgtgtcg ctggggttcc cccaggcggt cggcgacgaa tacgaacggc
    10981 tgcgccagcg attcctcgag ctgtatgcgt ccaacatcgc cgagcacacc agtctctttc
    11041 ccggcgcgga gagcctgctt gcggaactcg agcgtctcgc gatcccctgg ggcgtggtca
    11101 ccaacaagcc cggctggctc accgacccgc tgatgcagga gctcggcttg ttcgaccgcg
    11161 ccgcctgcat cgtgagcggc gacacggtcg cggagcgcaa gccgcacccg ctgccgctgc
    11221 tgcacgcgtc gcgactgctc ggcgtgccac cggtgcagtg cgtctatgcc ggcgatgcgg
    11281 agcgcgacat acaggccgga cgcgccgccg gcatgacgac ggtggtcgcg gcctacggct
    11341 acatcggctc cgacgaggac ccgctcgcgt ggcagcccgc ggggatcgtc acgcggcccg
    11401 agcacctgct cgattggctc ggcaccgacc cggcgcgggc cgtcgcaggt cggtcatgac
    11461 cggcggtgca accttgtggg tcatcttggt ggggctcctc gccttcggtg tcggctggct
    11521 gatcggccgc cgccggagcc acgcgctcga actcgagctc gctgcggccc gcgccgaggt
    11581 tcgctccgct gccgacgtgg cgcgcgaacg cgagcaggcc ctcgagctgg cgctcacgcg
    11641 gctgcgaacc ggcttcgaca ccgtcgcggg cgaggcgctg cgcggcaaca gcgagacgtt
    11701 cctgcaactc gcccgccagg tgctcggcca gcagcacgaa ctcgccacgc gcagccttgc
    11761 cgagcgcgag aaggccgtcg agacgatgct cgcgccggtc cgcgatgcct tgcagcgcac
    11821 ccacgagcag atcgcgcgca tcgagaagga acgggcggag acattcggcg cgctgcgcag
    11881 ttccatcgag ggcgtcgcgc tcggccagca ggcattgcag cgcgagaccc gcaacctggt
    11941 gacggcgctg cgtcgacccg aggtccgggg ccagtggggg gagatgactc tgcgccggct
    12001 cgcggaactc gcgggcatga tcgagcattg cgacttcgcc gagcaggtca cggtgcaggg
    12061 cgaggacggg tcgctgcgtc cggacatgat cgtcaacatg ccggatggcc ggcagctcgt
    12121 ggtcgacgtc aagacgccgc tcgacgcgta cctctccgcg gtcgaggcca cgaccgacga
    12181 ggaccgcgcg gccgcgatcc gtcgccacgc gcaggccgtc gtggagcgcg tccggcaact
    12241 tgcgtcgaag tcctactgga cccagttcga gcacagcccc gacttcgtgg tgctgttcat
    12301 ccccggcgac cagttcgtga gcgccgccat cggcgaagtg ccgacgctgc tcgaggacgc
    12361 gatccggcag cacgtgatca tcgccacgcc gacgagcttc atcgcattgc tgaaggcggt
    12421 cgcgtacgga tggcgtcaga acgcgctcgc agagaatgcg gcgcacatcc aggaactcgc
    12481 cgaggaactc tacaagcgac tcgcgacgtt cggcgagcac ctcgcccgga tcgggcgctc
    12541 gctcggacag agcgtggacg cctacaacag cgcggtcggc tcgctcgaac ggcaggtcct
    12601 gcccggcgcg cgcaagttca ccgaactcgg actgaggccc ggcaaggaaa tcggcgacat
    12661 tgcgccggta gataagctcg cgcgcgtgcc gaagtccggc gacggagacg gtgccgatga
    12721 gtgaggtcga cccgcgcgcg tatgtgccgc ctcctggcgc gctgcaggac cgggtcatcc
    12781 tcgtgacggg cgccagcggc ggcctggggt ccgaactcgc ccgcacgtgc gccgtgctcg
    12841 gggcgcgcgt ggtgctgact ggacgccatg taaagcggct cgaaacggtc tacgaccagg
    12901 tccgcgccgc gggtgggcca cgtccgtcga tcgtgccgct cgacttcgag cgcgctgatg
    12961 cgagccacta ctccgcgctc gtcgatgcgg tcgcggcgga gtatggccgg ctcgacgggc
    13021 ttgcgcacgt cgcggggctg ctcggtgacc gctcgcccat cgagcactac gacgtcgtga
    13081 cttggatgcg ggtgatgcac gtgaacgtca atgccgcatt catcctgacg caggcgttgc
    13141 tgcccctgct gcgggtctcg caggacgcat ccgtggtctt cacgacgagc ggcgtgtcac
    13201 agcgcgcccg cgcctactgg ggcgcgtatg ccgtgtccaa gttcgcgctc gaggggctca
    13261 tgcaggtcct ggccgacgag accgacacct cgacgcacat ccgcgtgaac agcgtcaatc
    13321 ccggccgcat ccgcacgtcg atgcgtgcga aagcctatcc gggcgaggat gccgcgtccg
    13381 tgccactccc gtcgcaggcg ctgccgacct acctctacct gctcggcccc gcgagtcgcg
    13441 gcatcaccgg ccgccgcttc gacgcccagt agggcggcgt ctcgggcgcg ccgccggtcg
    13501 cgcctcggcc tggcgcccgg ccaggcccag cagggcctcg agttccgtgc cgacgagttc
    13561 acgcgagcgc ccgcgcggca ggtcgcgcgg cagcgatacc ggaccccatc ggacgagcat
    13621 cgtgcggctg accttgagcc cggcggcgtc gaacaacgcg cggaccgcgc cgcgtggcaa
    13681 ggtccgctcc gccgtcacct tgaaccatgc gttggtcaag ccgccttcga ggcgctcgac
    13741 ggcggaaaat gcgacgtgct ggccctcgac ctcggtctcg agcggcagcg ccggccagtc
    13801 gtccgcctgc cgcggtcgca gggcccgtac gcggtattcg acggggatcg ccgcagcccg
    13861 gcgcgtgatg gccgacacca gcgtgccgtc gctcgccatg atcagcaggc cgtcctcgcc
    13921 gaacccgagc gcattgaccg gcaaccagcg acccgcccgc aggctcggca ggcgcatttc
    13981 cacgccggcg cgctcgtcgc cggcctgtcg gcgcagcatt tcgccgctcg gcttgtgata
    14041 gacgatcacc cgttgggacg gcgtcgacgc gagccgcttc gtgacgtcgc ggccgtccac
    14101 gaccacccgg tccccggggc gcaccttctg gccgatctcc gccggccgcc cgttgaccag
    14161 cacgcgaccc tcgccgatcc aggactcgac ctgccgtcgc gaacccaggc cgtgctgggc
    14221 cagcagcttg tgcaggcgct cgtccaagga tcgcgtctag cgctggtcgt cgccgggagg
    14281 cagcgccacc agtgtcggag cctcgtcttc cggtccggcg cggcccgctt cgagttcgtc
    14341 gtccggcgcg gcctcgtcgt cggaggcttc gtccgcgacc atcgaaccag ccgtcgccag
    14401 cacctttccc gaaagggcct caggcatcgt tggcatttcc tcgccgtcgg ccccgcccgg
    14461 cagctcgagc tgcaccttga tgtccccgag gtcctgcagc tctgccagcg tcggcaggtc
    14521 gtcgaggctc ctgagcccga aatagtcgag gaactcgcga gtcgtgccga ggagttccgg
    14581 ccggccaggc acctcgcgat ggccgacgac ccggatccag ttgcgctcct gcatcgtgcg
    14641 catgatcgtg gacgccaccg tcacgccgcg gatgtcctcg atctcgccgc gcgtgatcgg
    14701 ctgccgatac gcgatcagcg ccagcgtctc gagcagcgca cgcgagtagc ggctcggacg
    14761 ctcctgccag agcctggaca cggtggccga gtactgcggg cgcacctgca cgcgccagcc
    14821 gctcgccacc tcgcgcagct cgacgcccgt gccctccagg cggctgccga gttccgccag
    14881 tgcctcgagc acgtcttccg ccgcaggccg ctcgtgttcg tcgaagagct ccagcacctg
    14941 gtcgaccgtg accgggcgac ccgcggccag cagcaccgct tcgacgacgt tgcacagtgg
    15001 atggtcgctc atgattccgt ccggtttccg gcctccggcc ggtcgttgtc tacgacgagc
    15061 gtgacgtgcc tcggcgcgcc cgcgcgcacg tggatcggcg cgaactgctc cgcctgcacg
    15121 atgtcgatca acccttcgcg caggagttcc agcagcgccg agaaggtcac ggtgactccc
    15181 atgcgcccct cttcgggccg gaacagctgc gagaactcga cgaacgcctg cgcacgcagc
    15241 gccgccagca cgtccgacat gcgctgccgc acggagagcc gctcgcgctg cacgtggtgg
    15301 tgcgcgaaca tgtcgacgcg cgacatcacg tccttcagcg cgaccagcat ctcctgcatc
    15361 gtcaccgtcg gcaggatgcg cacgacgcga cgctcggtga gctcggccga cgccgggaac
    15421 gtgtcgcgat ccacgcgcgg cagttcctcg atgcgctcgg cagcccgctt gaagcgctcg
    15481 tattcctgca ggcgtcgtac cagctcggcg cgcgggtcgt cctcctccgc cccgccctcg
    15541 ccctgcgggc gcggcagcag catgcgcgac ttgatctcgg cgagcgtcgc ggccatcagc
    15601 atgtactcac cggcgagttc gagctgcagg tcgcgcatca cctcgatgta ctgcatgtac
    15661 tggcgggtga tctccgcgag cggaatgtcg aggatgtcga gattctggcg gcggatcagg
    15721 tagaggagca ggtcgagcgg gccctcgaac gcctcgagga acacctgcag tgcgtccgga
    15781 gggatgtaga ggtcgcgcgg catctcggtg agcggctcgc cctcgaccac tgcgaagggc
    15841 atctccgcct gctcgtgcac ttcgacgacg tgggcctccg gggccggcgg cggcaccttc
    15901 tccaccagtc gcaggtctgg cttctgcatc gcgaacctca gcggtatccg agacccatgg
    15961 cctgtttcac gtcgtccagg gtctgcccgg cgacggcccg ggccttctcg cagccctccg
    16021 cgagtatgcc gcggacgagg tccggattct cctcgaattc ctggccgcgc tgccgcaggg
    16081 cgctgacctc ggcgacgacc cgctcgatga gcggcttctt gcactcgagg cagccgatgc
    16141 ctgcggtgcg gcagccctgg tcgacccagc ggcgggtttc gtcgctcgaa tagagcttgt
    16201 gcaggtccca gaccgggcac ttttccgggt cgcccgggtc ggtgcggcgc acgcgggccg
    16261 ggtcggtctg catggtcttg agcttggccg cgacggcatc cgggtcctcg cgcatcccga
    16321 tggtgttgcc gtacgacttc gacatcttgc ggccgtcgag cccgagcacc ttcggcgtgg
    16381 gcgtaagcag cgcctcgggc tcgggcagga tggtccgccc cgtgccctcg aggtacccca
    16441 gcagccgctc cctgtcggcg agtgtcaggc gcgaattacc ctcgagcagc gcccgcgcct
    16501 tgccgagcgc ctcctcctcc ccgcgctcct ggtagcgctt gcggagatcg cggtagagcg
    16561 ccgcgttctt ggcgccgagg ttcttcacgg cacgctccgc cttcgcttcg aagtcggtct
    16621 cgcggccgta gatgaaattg aagcgccgcg cgatctcgcg ggtgatctcg acgtgcgcga
    16681 cctggtcctc gccgacgggg atgtacatcg gccggtacag caggatgtcg gcgctctgca
    16741 gcagcgggta gccgaggaac ccgtaggtcg cgaggtcctt ttccttgagc tgttcctgct
    16801 ggtccttgta ggtcggcacg cgctcgagcc agccgagcgg cgtgatcatc gagagcagca
    16861 ggtggagttc cgcgtgctcg ggcaccttcg actggatgaa cagcgtcgcg acggagggat
    16921 tgaggcctgc cgcgagccag tcgatgacga tctgccaggc gttcgactcg atcccggccg
    16981 tctcgtcgta gccggtcgtg agcgcgtggt agtcggcgat gaagaagtag cagtcgtact
    17041 cgtactgcag ctccgtccag ttgcgcaggg ccccgaagta gttgccgagg tgcaaggcgc
    17101 ccgtggggcg catgcccgac agcacgcgtc gggaggtcgt cactgaagaa ctcaaggcgg
    17161 tcccttggga atcgtcgggg aggcgggatt atacgcagcc ggccgcggcg gcggcgtcat
    17221 tcgaacgccg cgacgtcgcc ctttgcccgg cgcacgacga ccggcgcttc gcccgacagg
    17281 tccaccacgc tggagggctc gaacccgcag cggccgccgt cgatgacgag gtcgacggcg
    17341 tgctcgagcc gcgccttgat ctcctgcggg tccgtcatgg gcgtcggctc ccccggcagc
    17401 agcagggtcg agctcatgat cggctcgccg agctcggcca gcagcatgcg cacgatggcg
    17461 ttgtccggca cgcggatgcc gatggtgcgt ttcttcgggt tctgcagccg cttcggcacc
    17521 tcgcgggtcg cctccaggag gaacgtgtac ggtccgggcg tgaacgcgcg cagcgtgcgg
    17581 tactggcggt tgtcgacccg ggcgtacttg gcgatctgcg aaaggtcgct gcagacgaac
    17641 gtgaagttgt ggttcttgtc ggcctcgcga atgcggcgga tccgctccat ggccgccttg
    17701 tcgccgatca ggcaaccgag cgcgtagcac gagtcggtcg gatacgcgac cacgcttccg
    17761 tcccgaatgg ccgcgatcgc ctggcggatc agcctcggct ggggactcac cgggtgtatg
    17821 gtcaggagca gtgccatcgg cctaacggtc cgtcgttggg gccgacggtc gagttggata
    17881 tgatacgcgc cccgcccacg ggccgccccc ggccgccacc acagcgccca gcatgcagtt
    17941 cgtcgtcgac ctgttaccgg tcatcgcatt cttcgccgcc tacaagttca agggcatcta
    18001 cgtcgccacg gcggtgctca tcgtgggcgt gctgctgcag acggcggtga cgtggctgcg
    18061 gttccgcaag gtgagctcga tgctgctcgg gtcggccgcg ctcgtgctgc tgttcggcgg
    18121 cctcacgctg tacctgcacg acgcgacctt catcaagtgg aagccgacca tcgtcgactg
    18181 gctgttcgcg gcggtgctgc tgtacggcca gttcgcccgc gggcccaact tcctgcagcg
    18241 aatgatgggc gagcaggtca gtctcgtgcc cgccgactgg acgcgactca acgtggcgtg
    18301 gacggtcttc ttcctggtga gcggcgtcct gaacctgtac gtcgcctaca actacccgga
    18361 gtcgacctgg gtgagcttca agctgttcgg cctgatgggc ctgatgctgc tgttcgcggt
    18421 ggcgcagggc ttctggatcg cgcgccgggc catcgtcgcc gacacggagc aggcctgacg
    18481 tgctctacgt gatcaccggc cgcgatgcac cgggttcgct ggcgacgcgg cgccgcgcgc
    18541 ggcccgcgca cttgagcagg atccgtgaac taagcgacgc cggccgtgtc gtactggcgg
    18601 gcccgtgccc cgccgtcgac tcggcggagc ctggcgaggc cggattcacc ggcagcgtca
    18661 tcgtggccga ctttccctcg ctggacgctg cgcgggcctg gatcgccgcc gacccctacg
    18721 tgaccgacgg cgtattcgaa tcgcacgagg tctgcccgtt catcaaggtg ttgccgtgac
    18781 agccacgcgt ctcgaaagac tgacggagcg gctgaacgag gcgctggcgc cgcggcagct
    18841 cgacgtcgtc gacgacagcc atcatcacgc gggccacgcc ggagccgcgg atggccgcgg
    18901 tcatttcagc gtgctcatcg tctcggaccg attcgccgga ctcgggacgc tgaagcgcca
    18961 ccgactggtg tacgacgcgg tcggcgacat gatgaccacc gacattcacg ccctttcgat
    19021 ccaggccatc gcaccgggcg aggccaccac ctgacaggat tccaacccca gaggctcatg
    19081 atgcgtacgc aaagattccc cctcgttgtg ttgatagccg gcattgcatt gctggctggc
    19141 tgcaccaagc cggccggcga agccgcaaag cccgctgcca gcgccgccag ccccgccggc
    19201 aagcctgcgg ccgacgtcgc caccgtgaac ggcaagccga tcaccgcgga acagttcagc
    19261 gtcttcgtcg aagcggtgac tgggaagcca ggcaccgacc tgccgcccga gcagaagtcg
    19321 cagatgctcg accagctcat caacatggag ctggcatccc aggcagccga gaaagaaggc
    19381 ctgcagaacg atgccgccac caaggcgcgc atcgcgctgc tgacgatgca ggtgctcgcg
    19441 caggccggca cggagaagta cctgaaggcg cacccggtgt ccgactccga ggtcaaggcg
    19501 gagtacgacg agcaggttgc gaagatgccg atggagtaca aggcgcgcca tatcctcgtg
    19561 gacagcaagg agcaggccga ggcgatcatc aagcagctcg acgccggcgc cgatttcgcc
    19621 aagctcgcag ccaaggactc gaaggatccg agcggcaaga atggcggcga cctcggctgg
    19681 ttcagcctgc agtcgatggt gaagccgttc tcggacgccg tggcggccct gaagaagggc
    19741 gagatcacgc agcagccggt gcagacccag tacggctggc acgtgatcca gctcgaggac
    19801 acccgcacgc cgaccgcgcc ggcattcgac gacgtgaagc agcaggtcaa gacgctcgcc
    19861 gagcgcaaga agctccaggc ttacctggac gagcttcgca agacggccaa gatccagaaa
    19921 acaggttaac tgtcaagccg tcattcggct ccgcacggcc ggattccctc gggcgtccgg
    19981 ccgtattttt ttggcttcga cagtacgcgc cggacccagg tagtccactc gacaattgcg
    20041 agcgtctgcc tttggtggcg gacccctcgg ccgctgttcg ccggacccat acggagcgtt
    20101 cggcagcgcg ctccagacgg ccgccagcca gaattcgcag ccgcctagag aagttctagg
    20161 ttttcagcat aagtcactga ctatttttta aaatcctctg ggctggcagg aaagtgtgac
    20221 gggattcaca tttatccggt gcggcacttc attaggtcaa aacagcgccc ctcgctgact
    20281 tgacatagaa cgcctccggg atcgaattcc cgctgagcca gcgctgctgc aagcgtttct
    20341 aggcaggcac cctcactact ttgaatttgc gggtatttcg caacggaccc gccggccggc
    20401 ccattcaaga cgacctatca gaaccaggcc gtaagtgtcc gaatatgttg aactcttgac
    20461 cgacccagcc acgccgatat agttcaacgc gggagttaac aataagtgta atgacagcgg
    20521 cagttgcaca ttggacgggg ccgcccgaat tcgggcttgg aatgagcgag aacgttaaaa
    20581 aatcgttgct tgcggcgtgt cggcacctgc tgcacccgct ggttcgaatt ctgctgcgtc
    20641 acggcgtcgg ttacggcgaa ttttcggact cagttcgtgg cgcgtacatc gacatcgctt
    20701 cggccgaact gatgaatccg aatcagccgc agagtgatgc gcgactcgga atcctcactg
    20761 gaattggcgc ccaagaagtg caccgaatcc gggaagccga tgcggcacag gagtcggaag
    20821 tcggtctgaa tcaaatcgct cgagtcctgc aagggtggag ccaggacccg gagtacttgg
    20881 gtccgtacgg cctgcctctg gaggttccat ttgcgggcga ccgaatctcg ttcgaggcgt
    20941 tggtcggccg ctatgccgta ggcgccgaac cgcgcccgct actcggggag ctaatccgcg
    21001 tcggcgcagc cgtggaagcg gacgacggct atgtgcggct cctcaatcgg acgtatctac
    21061 cgacgccatt ggacccggtt ggtctcgagc gcctcggcaa cgtcgtcaat tacttcatag
    21121 ataccgtcga cttcaacttg cagaagaaga ggcaaggcga agggcggttc gagcgatacg
    21181 cgataacgat ggaaggcctg tcgcccgatt cgttcgcagc ttttgaccaa ctcgtgcgag
    21241 agaaaggcca ggagttccta gagatcctag acgactggct tggtcaatac gagatcaagg
    21301 gcggccatcg cttgccacct gacgcatcga ttcggacggg agtcggagtt ttccacttcg
    21361 tcgatcgttt cccaacgacc acagacgaca gctttcgata gaggcagcca cgcaaatggt
    21421 cgacagcgac accaaggata aaaggggatt ggggatgagc acagccggtc gagttcaaac
    21481 aattgcgcta gctgtggggc tcgtgttggc ggcagggtca acatccgcag ccaaccgcgg
    21541 caacgccatc atcggcggcg acctcgccaa ggcggccccc agcgccatca tcggtggcga
    21601 cgccatcatc ggcggtgacg cgatcatcgg tggcgacgcc cgcaaggctc gccgcggcaa
    21661 cgccatcatt ggtggcgatg ccatcatcgg cggcgacctc gccaaggcgg cccccagcgc
    21721 catcatcggt ggcgacgcca tcatcggcgg tgacgcnnnn nnnnnnnnnn nnnnnnnnnn
    21781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    21841 nnnnnnnnnn nnnnnncgcc gcggcaacgc catcattggt ggcgatctgc tagcgaccta
    21901 tccgtccaac gccctcgtcg tcggtccggt cgaaaaggtc agcgtcgcca ccggccaagt
    21961 tacgattctt ggccagacat tccaggggcc agcgggaagc cgccagctgc aaagcatgtc
    22021 cgagcagttg gcatccggaa caccggtgct tgcaaccatt acaggtgctc ccgacaagtc
    22081 gggccatctg cacccaatta gcatgacagc caactcgggc ccttacgccg caggagcttc
    22141 acgagtcctc gttgtaggtc gtgtgtcttc gaccagcctt gcgacgggtc atttcagtgt
    22201 tggacaactc gtagtcgact acacgtcgct gctagcggac tcaagtgtcc aggtcgcgtc
    22261 cggatctacg gtcgcgatag tcggcgtcca agccgcaaaa ggcggggcgc tccaagcgct
    22321 gcaacttcgc gtacttcgct aggcctacct gcgcaagcat agagggcacc actaggtgtg
    22381 gtgccctttg tttatctgca aaatggttct gtagcggcgt tcattctgct ccggacggcg
    22441 cggtcccctc gaagtagaac actcctacgc cgactctaag ggattcatcg gcatgcgccc
    22501 cagccgcgat ctcattggaa ctcagccagt catcgatgga ctgaacgaag tccgagcatc
    22561 ggctccgcgc aatgtgccgg aagagcggta ccgccgccgg caataaccga tcggagtacg
    22621 ccagccgctg cacaaatggc tcgacgtttt cgtcgccagt gttccgaacg agcccttcaa
    22681 ttaccggctg aacgatctgc gtcaatccaa ttacaaggtc ctcgttgacg ttgcctggca
    22741 caaaatatct cttgactgct cggagtccac caccggcggt ctcctcgacc gacccggcag
    22801 cgaccaattc cgctcgaacg gcacctgcag gcacgtcgcc gccaaccaac cgcaccaggt
    22861 tagtgaatgt cacgccatcg cctgcgaaac gcagttcgct aggttcgccg gacgagtcca
    22921 caaagcgggc gtccgaatac cagagctgaa gcactcgcgc cgcgtggcct gcgtgcttga
    22981 tctcgccgac caactcaaca accgtccctt tctccaacgt gcgccgatct cttattcgag
    23041 cgacctcttt tcgagagagc ccggtccgta tcgccattct ggatacattc gcaaccctac
    23101 cccgagcgtc tcgttcgctc gcggcctctt ccaagaacgc tatctttgct gcgtcggcga
    23161 attgtcggta attgatgccc gaccgcagta gcagacgcgc caacggacgc aagcacatca
    23221 ttgttgccgc gagaaccttg tcttgaatgg cagcgctcat gcctacgtca gctcgatttc
    23281 tggtatttga aaacgttgat gaccccagct ggatagcgcg gtcaattcgc gattggctcg
    23341 attattcgac gttgaatagc gcccgtcgat aatgtcgcag ctccatcacc gactcccgaa
    23401 cgtcctcgag cgccgaatgc gtcgaagcct tctgcacgcc ggcgagcacg tcgggcgccc
    23461 agcggcgcgc gagttccttc agcgtgctga cgtcca
//
LOCUS       DAOMFD010000013        22172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_402_length_22172_cov_31.474612, whole genome shotgun sequence.
ACCESSION   DAOMFD010000013 DAOMFD010000000
VERSION     DAOMFD010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 22172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_402_length_22172_cov_31.474612"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..981
                     /locus_tag="PL152_01610"
     CDS             <1..981
                     /locus_tag="PL152_01610"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CorA family divalent cation transporter"
                     /protein_id="HQR47998.1"
                     /translation="LVHEQAEEAGLIRFRLLEYYRMPLMAYLALDDPRALTRDDFVRL
                     GLVTAPGTDTPLPYSDRHLADFEDRYCYDRFWSSRPDGPSTRYLCCGHALVVVGSNGS
                     AAFLNPERGVLAQFRHQHFLLFLIAHFQRAALLMFSDRLVQALKKLDIHDPESVKRFK
                     RSIRQNFEIFLRFTHRYWFHEVADQAQARALFELTARHLGLDALYGEVKERIYDMNEY
                     LDSDSLRRQANTVLRLTVVTTFGLIGTVVTGFLGMNLIAAADQSFGHKLAYMLLVLVP
                     TTLLTFYTVSRSKPLSDFLEALSDERLGVGDKLRALAAVWRRPPRRPPRG"
     gene            complement(1021..1392)
                     /locus_tag="PL152_01615"
     CDS             complement(1021..1392)
                     /locus_tag="PL152_01615"
                     /inference="COORDINATES: protein motif:HMM:NF022317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2784 domain-containing protein"
                     /protein_id="HQR47999.1"
                     /translation="MTPRLLADLVLLLHAAFVLFVVLGALLVLRWPRVAWAHVPAALW
                     GACIEFAGWICPLTPLENHWRRLAGEEGYAGGFVEHYVFPVLYPDGLTRNAQLVLGAL
                     VLLINVAAYGWAIRRRRRPAR"
     gene            complement(1394..2047)
                     /locus_tag="PL152_01620"
     CDS             complement(1394..2047)
                     /locus_tag="PL152_01620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48000.1"
                     /translation="MTESARLALVGLRDFSTLQWYAVPLLALVFYVYVVEIRAARQSG
                     NWDAVIAGATLFGADFVNESINGWIFAISGYSALWLTPGPTALRTFVGWNLEIMFMFA
                     IAGILYCKSLDADPRARVLGLPNRWFWALFYSAVSVAVELALNSGGLLVWDYRWWNRG
                     VLAIVPIFVLGYLWFYLAAKYAIERPTLAGKVRVPVALFALAVALDVVGLGLLGWTY"
     gene            complement(2064..2357)
                     /locus_tag="PL152_01625"
     CDS             complement(2064..2357)
                     /locus_tag="PL152_01625"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HQR48001.1"
                     /translation="MRVLFHASIVPVVLIAAAGSAHADDDFRCSGRIVKAGMTDAAVL
                     QACGAPESRQSETQDVRSGNRVVGKTVIWRWTYTIAGRTRVLVFDQGALKSIE"
     gene            2515..2946
                     /locus_tag="PL152_01630"
     CDS             2515..2946
                     /locus_tag="PL152_01630"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQR48002.1"
                     /translation="MAGGTYKAGCPCGAVQLELSGEPAVQVYCHCDSCRTWLSAPIHG
                     ATLWPTTNVKITKGADNLGMYKRTDASHRQFCKSCGAAVLVGHPAIGMTDVPSVSIKG
                     LAFSPALHVNYSEKVMAVHDGLPKFKDFPKEFGGSGEMLPE"
     gene            2988..3353
                     /locus_tag="PL152_01635"
     CDS             2988..3353
                     /locus_tag="PL152_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48003.1"
                     /translation="MATRFTAWLSGKLGRNEPEQPKEAPAARKSAEPYHAVTIKAGAE
                     SCEGAKQLAGVRFLSTSAPRLPLPNCSQPACLCHYIHHADRRTGTDRRAAENWRRTRD
                     SGVERRQRKQGRRANDSIA"
     gene            complement(3468..3932)
                     /locus_tag="PL152_01640"
     CDS             complement(3468..3932)
                     /locus_tag="PL152_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HQR48004.1"
                     /translation="MSRAFVKEQDGAESDDLPERPVSRHRNLVTPEGLIRIEQMVRGL
                     GLQLAEARAADDRAAVARVERDLRYWTRRKATAELVPRTPRPERVRFGCRVELETDTG
                     ERVSFRIVGEDESDPSQGLISYVSPLAELLIGSKPGDRVSFRQGQAEIVGID"
     gene            complement(3950..4441)
                     /locus_tag="PL152_01645"
     CDS             complement(3950..4441)
                     /locus_tag="PL152_01645"
                     /inference="COORDINATES: protein motif:HMM:NF015147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LEA type 2 family protein"
                     /protein_id="HQR48005.1"
                     /translation="MRAALLRGWLATVSLALASCAGLPHGDPLQVSVAGIDSLPGEGL
                     EVRMALKLRVQNPNDAEVDYDGAYVALSVQDRTFATGVSDATGHIPRFGESVISIPVT
                     VSVLRLVGHVVGSAGGTTSGPIRFSLEGKLSGSGLSTYRFKSQGELQLPASRSEPVEP
                     DQP"
     gene            complement(4452..4838)
                     /locus_tag="PL152_01650"
     CDS             complement(4452..4838)
                     /locus_tag="PL152_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48006.1"
                     /translation="MIGGAGKGPGREDAWPVIEELEDPVEAELVSPASLLRFLGETSH
                     VSEAGEPEPARSEPVPRITRMPEGVRVEERRFGRAVAQWILQVRCECGRRWFEVEEID
                     TAKCPKCGMLVYVDMEARTRPSRRTP"
     gene            complement(4900..5307)
                     /locus_tag="PL152_01655"
     CDS             complement(4900..5307)
                     /locus_tag="PL152_01655"
                     /inference="COORDINATES: protein motif:HMM:NF020839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR48007.1"
                     /translation="MLAAHRDDATAELFGITQLVREFGVSARAIRFYEDKGLLSPRRV
                     NGARVYTRRDRARLALILRAKAIGSTLAETKHYLDLYGARGEGRVQQMKYVLSRTDTA
                     IEELEQRRAHIETTLAELRLINDTVRAHLAGKA"
     gene            complement(5405..5701)
                     /locus_tag="PL152_01660"
     CDS             complement(5405..5701)
                     /locus_tag="PL152_01660"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQR48008.1"
                     /translation="MDMQQCTETIRGKVGEASGLDAVLKFDCGADGVIVIDGKSVPNT
                     VSNENRDADCTISITRENLEALLTGQLDSITGFMTGKFTVAGDMSIAMKLQRVV"
     gene            5858..6757
                     /locus_tag="PL152_01665"
     CDS             5858..6757
                     /locus_tag="PL152_01665"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR48009.1"
                     /translation="MSHAPDAPDPEPTPRYRSVFRPGLFAGRIAWVTGGGSGIGRCVA
                     HELASLGALVVISGRTASRLDGVAAEIAADGGRCETIAFDIRDEEAVKASVAATVQRH
                     GRVDALVNNAGGQFPSPLVAISKRGFDAVVANNLTGGFLMMREVFGQSMQKHGGAIVN
                     MTADFRNGMPGMGHSGAARAGMSNLTMSAAFEWAHAGVRVNAVAPGWVASSGMDTYQG
                     AVRAMIPKLKAHVPLRRLATEAEVSAAIVFLLSPAAAFVTGVTLQIDGGASLGSAVFP
                     SIDHDRSQPFDGFHRAVTPEALR"
     gene            6757..8376
                     /locus_tag="PL152_01670"
     CDS             6757..8376
                     /locus_tag="PL152_01670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQR48010.1"
                     /translation="MPAIVSQLDPRSATFKSNADRMAARLAEIRALEARVVEESASKR
                     AKFEERGQLLPRERVARLLDRGSDFLELSALAGLGMHDDDGRKSIQGGGSIVGIGVVA
                     GKRVVVSASDSAVKGGTVAPMGLKKGLRVQAIALQNKLPLVSLVESGGANLMYQAEIF
                     VDGGRSFANQARLSAAGIPQVAVVHGSSTAGGAYLPGLSDYVVLVRNRSSIYLAGPPL
                     VKAAIGEDATDEALGGAELHATVTGLGEYLAEDDAHAIAIARELVAALRWDEVAARPS
                     SVLPRYSSEELMGIVPTDERTPYDAREVIARIVDASDFLEFKAPYSTDTVCGHARIDG
                     HRVGIIANNGPIQPTGSVKAAQFMQLCDQTGTPLVFLQNTTGYMVGTAAERAGAIKHG
                     SKMIQAVANARVPKFTIVLGGSYGAGNYGMCGRGFDPRFIFAWPTARTAVMGGAQAAM
                     VMEMVSRGKLAKSGVPVNEQAEAGLKAMSDQLKRRLDAESDVLYGTARLWDDGVIDPR
                     DTRRVLALCLAIASEADARTLHPNSFGVARM"
     gene            8411..9565
                     /locus_tag="PL152_01675"
     CDS             8411..9565
                     /locus_tag="PL152_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020055843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48011.1"
                     /translation="MQFTDQHRQLDDTVTRFVANEINPHIPEWEKAGEFPSHEIFKKL
                     GDLGLLGLKYPEEYGGLGLDFSYSMVMAEALGAADCGGVPMAIGVHTDMCTPALARFG
                     TDQLRREYLAPSIAGDVVGCLGISEPSGGSDVAAVKTTARKDGDDYVVNGTKMWITNG
                     MKADWCCLLANTSDGSPHRNKSLIIVPMDAKGVTRQKIHKIGMHSSDTAQLFFDDVRV
                     PRRNLIGQEGMGFTFQMLQFQEERLWGAASSLRSMDRIIDLTIEYARERKAFGRSILD
                     NQVVHFRLAELRTEIAALRALTYQAVEQYVAGKDVTKLASMAKLKCGRLSREVADSCL
                     QYWGGMGYTAENLVARSYRDSRLVSIGGGADEVMLGIIAKLEGTLPRAAS"
     gene            9568..10365
                     /locus_tag="PL152_01680"
     CDS             9568..10365
                     /locus_tag="PL152_01680"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR48012.1"
                     /translation="MNDASTLAIRRAGAVLHVALNRPELRNAMSLAMVRELTTTLHAA
                     ESDGETRVIVLRGAGGHFCAGADLKDMAGARARLTQDPDAILKVNAAFGALCNAYAAS
                     GLAIVAVLEGAVMGGGFGLTCVADVVIAGDSTVFRLPETSLGVLPAQIAPFLVERLGY
                     SEAKRLAVTGSRLDAREALAIRLVHEVHAAQDLDAALARQLQDILACAPQALAATKAL
                     LRRARWTPGADLVQEAAAAFSRAALGPEGTEGTTAFLQKRKPNWAPQ"
     assembly_gap    10463..10562
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10563..12182
                     /locus_tag="PL152_01685"
     CDS             <10563..12182
                     /locus_tag="PL152_01685"
                     /inference="COORDINATES: protein motif:HMM:NF014805.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HQR48013.1"
                     /translation="AMGDKAGAKRRMLAAGVPCAPGYLGDDQDDGRLLAEGAKLGVPL
                     LVKAIAGGGGRGMRLVRSLDELPAAVAGARREALSAFGDGALMLERLIEGGRHIEIQV
                     FADRHGNAIHLGERDCTTQRRRQKVVEEAPSPIVDAAMRARMGADAVAAARAVGYVGA
                     GTVEFIVDARLQHYFLEMNTRLQVEHPVTESICGLDLVEWQLRVAAGEPLPLAQEDVR
                     FTGHAIEARLYAEDPYAGFAPQTGRILHWRPERAEARAVRVDSGIAEGGTVSPYYDAM
                     VAKVVAHGRDRADAARRLATAVEDAVLVGVKNNGRFLRDLLSHPDFTASRMDTGTLDR
                     WSESGEPVLQAPAIPETVWRLAAAVLATRNGTGWRPASVASYDLELISGDERRNLRVR
                     AQARSVDVTSHDATSSIEILSWRDHDLRYAEGGRMHTTVCVLEDDALHIASDGAVFTI
                     REVSPWPSRDANADPRYARAPVAGAVAAISVAPGDRVESGQPLVCVEAMKMEMWLTAR
                     SAGTVRAVHVEIKQSVEAGVLLVEIETEGTE"
     gene            12182..13051
                     /locus_tag="PL152_01690"
     CDS             12182..13051
                     /locus_tag="PL152_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HQR48014.1"
                     /translation="MDKAILTCALTGVLTNPKQHPVPVTPAQMAAEARDAFNAGASVM
                     HVHLRRQEDGMGHLPSWDPEVAAAVIDAIRAACPGVIINLTTGVIGKDISGPLACLRR
                     VRPEAAACNAGSLNYLKIKDDGGWAWPPMVFDNPVEKIQQYLGVMNELGIHPEFECFD
                     VGIVRSVGMYLKAGMFSGVPEVNFVMGVASGMPCDADLLALLPGYAPPGAVWQATLIG
                     RGEIWPVHQKAADLGGMLRTGLEDTFYLPGGERASGNGVLIEALARCARNAGREIATP
                     SEARVLLGVHPSG"
     gene            complement(13065..14744)
                     /locus_tag="PL152_01695"
     CDS             complement(13065..14744)
                     /locus_tag="PL152_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR48015.1"
                     /translation="MNSEVRPDDLQLQRLYRWERTIPDRVALTQPMGGGIVRDLTWRE
                     MMDETRRMAAHLKSLGFEPGARIGLLSKNTAHWLMADFAIWMAGYVSVPLYPSLAAGT
                     IRQILTHSEARLLFVGKLDGWDAMRDGVPTGLPMIRFPLSPPVDCPAWDEIVTSTQPL
                     QGEPVRDGNELATIIYTSGTTGTPKGVMHSFATFAWSIGTVLRRIGAHENGRVLSYLP
                     LAHVAERTVVEHGMLGLGLHVYFAESLETFAADIRRARPTIFFSVPRLWVKFQQGVLA
                     KMPAAKLDRLLRIPVVRRIVARRILRALGLDQCEFAAGGAAPMPAELLKWYRRLGLRL
                     SEVYGMTENCGVSHATLPDVPRPGTVGPGYDGIQSRVDPETGELQVKCPGVMLGYYKE
                     PELTRQAMTGDGWLRTGDKGEFDAEGNLRIVGRVKDLFKTGKGKYVAPAPIEDRLVTH
                     VAVEACCVTGANLGQPLALLMLNAETSQRIAEPAHRDDLEQSLSAHLDAVNALLDPHE
                     QLDCVVVVRDTWTVENDLVTPTLKVKRNRIEAVYATHYESWVGKRRPVVWQ"
     gene            complement(14813..16243)
                     /locus_tag="PL152_01700"
     CDS             complement(14813..16243)
                     /locus_tag="PL152_01700"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQR48016.1"
                     /translation="MTGPRIAGRLAGLVACAGCAYSSMVCAAAVASDDGEATFRSIYK
                     ELVEINTTLSSGSCTQAAEAMAARLRAAGLPKDSMEILAPPDRPKSGALIATLPGQDP
                     SLAPVMLLAHIDVVEARREDWSRDPFKLVEEGGYFYARGSSDDKAMAAVFTDILVRFQ
                     AQGFKPKRGVKLALTCGEETPDTFNSVHWLMQTRPDVLRAAFVLNEGAGGLLGPDGKP
                     VALDVQAGEKTYQDFMLETTNPGGHSSRPMRVNAINELAAGLTRLAAYQFPVQLNDTT
                     RAYFQAQAQLQPPDVAGAMRAIVANPADAAAAGKLWDANPSWNGMLRTTCVATEIEGG
                     HAPNALPQRARANVNCRILPGVPIESVKAELERVVADPGIKVSLVGDPGVMAPVPPLT
                     PAIMDPVRKVAAGIWPGVPVIPTMSTGATDGRFLNGAGIPTYGMSGMFSDAEGSRAHG
                     LNERMRVQSLMEGRRFLYEVVKIYVQ"
     gene            complement(16320..17642)
                     /locus_tag="PL152_01705"
     CDS             complement(16320..17642)
                     /locus_tag="PL152_01705"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HQR48017.1"
                     /translation="MSDDPKRKRSAVPASRASRLLRFGLLSGELALSAAAATARQLTS
                     GKRPDLAAAVLTPGNAGLLARRLAALRGPAMKVGQMLSLQSDEIIPAEFRNALSMLRS
                     QGYAMPDSQLRRVLGREYGRGWEAKFARFDMEPAAAASIGQIHRARTHGGRELALKVQ
                     FPGVARSVDSDVDNLATLLRRLDFLPVTIDIGAIAAEAKRQLRLETDYEGEARNLERY
                     ARLVADMPEVVVPRIHRGLTTRRILAMDWMDGEPLEGLASEAVPQATRNAVAKVLQEL
                     MFRELFEFRFMQTDPNFANYLYLPATQQIGLLDLGSVGEFSREFTDNYRRISRAVVAD
                     DRVAIRDAAAAIGYLHPDDAAERARGVVDIIRLVCEPLAHRGPYDFAESGMIGRVRDL
                     GLAVAFDQGLRSPPPQTIFLHRKLLGTFLICAKLRARVNVHALVERFV"
     gene            complement(17688..18581)
                     /locus_tag="PL152_01710"
     CDS             complement(17688..18581)
                     /locus_tag="PL152_01710"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HQR48018.1"
                     /translation="MAASVHLRLRKLPTGLATYPRVVLTKRPAITEVVDPPVFTANVS
                     GVALSRVSVAKYAKVCGYGDCIPPTYLHVLAMPLHLEIFACASFPLRPMGLIHLSNVI
                     EIRSALRYGMRLDFSVSARNYRRTDAGLAFDMATEISHEGRPVWLETCVFLSRWPESA
                     ERGGVRPPRPPKAPKDAAVLAELDVTSRTAWDYARVSTDYNPIHLSDRIARYLGLRGA
                     ISHGMWSLARSLAQPPVPALAAGTTIETQFLTPVQLPARVAIKEWMAEGQQRRALCDV
                     RTGRLHMYSSWSVVPPAQHAA"
     gene            complement(18584..>19246)
                     /locus_tag="PL152_01715"
     CDS             complement(18584..>19246)
                     /locus_tag="PL152_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48019.1"
                     /translation="VLTDDPSLQARAKDIIDATLVADPNNGKALWYSGVMAMRRGDKD
                     TAKAAFQKLLEQNPPPQIRQIVVEQLAGLGVQVPAAEGEGASAMAGSADAQSGQGQGN
                     VTPAGRTLRVSISVDPSVAAKLKPGMPLFVAAREPGIPGPPLAAVRLSSDQLPTTVVL
                     SDANSMVEGRNLSSVDTVEIVAHVAIGGTPTVATGDLVGQVKNSKGGPTDVAIVINQV
                     AP"
     assembly_gap    19247..19346
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<19347..19854)
                     /locus_tag="PL152_01720"
     CDS             complement(<19347..19854)
                     /locus_tag="PL152_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48020.1"
                     /translation="MTAFIVACIVMVCAAVALVAVPLARKQPVAAKGEPASPRSTSAF
                     AVVAITLPLAAGALYWGITNFPWKNPEAASAVPAGHGEGEAGSLADAVKQLEARMAAN
                     PQDAEGWQMLGRSYLVTGQAGKAVTAYEKADAIVGGTDPGLKLDLAEALVLTDDPSLQ
                     ARAKDIIDA"
     gene            complement(19851..20282)
                     /locus_tag="PL152_01725"
     CDS             complement(19851..20282)
                     /locus_tag="PL152_01725"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HQR48021.1"
                     /translation="MLRALLLAIAFAASGTAHAIDTERAFEDPALQARYENITRELRC
                     LVCQNETIADSNATLAGDLRREVREMIAAGKTDDQIRQFMLDRYGDFVLYRPRMTPLT
                     FLLWSAPVLLVALGALAVVRVIRRRSTEADTDPEGQEDGQS"
     gene            complement(20288..20830)
                     /locus_tag="PL152_01730"
     CDS             complement(20288..20830)
                     /locus_tag="PL152_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008810878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HQR48022.1"
                     /translation="MWKFIIPLGIFVVIGGFLLVGLNRDPSYIPSPLIGKQAPAFSLP
                     SLQDASYPVSSGDLKGQTWVLNVWGTWCGGCRQEHPVLLEISRQKAVPMIGLDWKDDS
                     VAARQWLSELGNPYSAVASDQEGRVAIDWGVYGAPETFLIGPDGVVLYKHIAPMTMEV
                     WVREFLPRIEAAKAGKGGAT"
     gene            complement(20845..>22172)
                     /locus_tag="PL152_01735"
     CDS             complement(20845..>22172)
                     /locus_tag="PL152_01735"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HQR48023.1"
                     /translation="TISWLFLTMGIAGGSWWAYYELGWGGWWFWDPVENASFMPWLVG
                     TALIHSLAVTEKRGLFKSWTLLLAIIAFSLSLVGTFLVRSGVLVSVHSFASDPKRGLF
                     ILAFLGLVIGGSLALFAWRGPALRAKGGFEMLSRESFILFNNMLLVVASALILIGTLY
                     PLFLDALNLGKISVGPPYFNVVFLIPMVPLMVLLAVGMHAAWKKGRLADMRKPLLVML
                     GASVAFALLVTVAAYRDFTVMSAVGIAAGTWVILSSLYEPVQRWRKKQTISRGMVGMT
                     LAHIGVGLFALGVTITQNYRIEKDIALKPGQSVSIHGYEFQFVSTREVPGPNYQAIEA
                     EVAIKRDGRTVKVLHPQKRVYRVQRSPMTEAGIDVNWNGDLFVAMGEDLGAGAWSMRV
                     QYKPMVRFIWLGAIVMALGGLIGITDRRYRTRREAATEKVGAGAPARAR"
BASE COUNT         3509 a         7669 c         7434 g         3360 t
ORIGIN      
        1 ctggtgcacg agcaggccga ggaagcgggc ctgatccgct tccggctgct cgagtactac
       61 cgcatgccgc tcatggccta tctcgcgctg gacgacccgc gtgcactgac gcgtgacgac
      121 ttcgtccggc tcggcctcgt cacggcgccc ggcaccgaca cgcccctgcc ctactccgac
      181 cgccacctgg ccgacttcga ggatcgctac tgctacgacc gcttctggtc cagccggccc
      241 gacgggccgt cgacccgcta cctgtgctgc ggccacgcgc tggtcgtggt cggcagcaac
      301 ggctcggccg cattcctgaa tcctgagcgg ggcgtcctgg cgcagttccg tcaccagcac
      361 ttcctgctgt tcctgatcgc gcacttccag cgggccgcgt tgctcatgtt ctcggaccgc
      421 ctcgtccagg cgctcaagaa gctcgacatc cacgacccgg agtccgtgaa gcgcttcaag
      481 cgctcgatcc gccagaactt cgagatcttc ctgcgcttca cgcaccgcta ctggttccac
      541 gaggtcgcgg accaggcgca ggcccgcgcc ctgttcgaac tcaccgctcg gcacctgggc
      601 ctcgatgcgc tgtatggcga ggtcaaggaa cgcatctacg acatgaacga gtatctcgac
      661 agcgacagcc tgcgccgcca ggcgaacacc gtcctgcgcc tgacggtcgt cacgacgttc
      721 ggcctgatcg gcaccgtcgt caccggattc ctcggcatga acttgatcgc ggcagcggac
      781 cagtcgttcg gccacaagct cgcctacatg ctgctggtgc tcgtgccgac cacgctgctg
      841 accttctaca cggtctcgcg ctccaagccg ctgtcggact tcctcgaggc gctctcggac
      901 gaacgcctcg gcgtcggcga caagctgcga gcgctcgccg ccgtgtggcg acggccgcct
      961 cgccgtccgc cgcgcggctg aaccagtgcc gcgaccacgt gcgccgcgcg cgactcgacg
     1021 tcatctcgcc ggtcgacgtc gacgccggat cgcccagccg tacgcggcga cattgatcaa
     1081 gagcacgagg gcgccaagca cgagttgcgc attgcgcgtg agcccgtccg gatagaggac
     1141 cgggaagacg tagtgctcga cgaacccgcc tgcgtagcct tcctctccgg ccagtcgccg
     1201 ccagtggttt tccagcgggg tcaacgggca gatccagccg gcgaactcga tgcacgcgcc
     1261 ccacagcgct gccggcacgt gcgcccacgc gacgcgcggc cagcgcagca ccagcagtgc
     1321 cccgagcacc acgaacagca cgaatgccgc atgcagcagc aggaccaggt ccgcgagcag
     1381 gcgcggggtc acctcaatag gtccagccca gcaggccgag cccgacgaca tcgagggcca
     1441 ccgcgagcgc gaacagcgcg accggcacgc gcaccttgcc cgcgagcgtg ggccgctcga
     1501 tggcgtactt ggcggcgaga tagaaccaca ggtagccgag cacgaagatc ggcacgatcg
     1561 cgaggacgcc acgattccac cagcggtagt cccataccag caggccgccg ctgttcaagg
     1621 cgagttccac cgcgacggac accgcggaat agaagagcgc ccagaaccag cgattcggca
     1681 ggccgagcac gcgtgcccgc gggtctgcgt cgagcgattt gcagtacagg atgccggcga
     1741 tggcgaacat gaacatgatc tcgaggttcc agccgacgaa cgtgcgcagc gcagtcggac
     1801 ccggcgtgag ccagagcgcg ctgtaaccgg agatcgcgaa gatccagccg ttgatcgatt
     1861 cgttcacgaa gtcggcgccg aagagcgtcg cgccggcgat gaccgcgtcc cagttgccgc
     1921 tctggcgggc agcccggatc tcgacgacgt acacgtagaa gaccagtgcg aggagcggca
     1981 ctgcgtacca ttgcagcgtg ctgaaatcgc gcaggccgac cagggcgagg cgtgccgact
     2041 cggtcatcgc ggatacctcc tgcctactcg atggacttca gggcgccctg gtcgaacacg
     2101 agcacgcgcg tgcggcccgc gatggtgtag gtccaccgcc agatgacggt cttgcccacg
     2161 acacggttgc cgctgcgcac gtcctgcgtc tcggactgcc tggactccgg cgcaccgcag
     2221 gcctgcagga cggcagcgtc cgtcatgccg gccttgacga tgcggccgct gcagcggaag
     2281 tcgtcgtcgg catgcgccga ccccgccgct gcgatgagta cgaccgggac gatggacgcg
     2341 tgaaacagga ctctcatggc cagaccccac atggtccccg gcggttgccg ggctccgacg
     2401 tgcccgcggg cgccggacga ccaccggcac gccgattgca caccactcgc agcaagtttg
     2461 acattccttg cggtccgcgg agaatcgcgt cattcgaata aggggggatt tccaatggct
     2521 ggtggaacgt acaaggccgg ctgcccgtgt ggcgccgtcc agctcgaact cagcggcgaa
     2581 ccggcggtgc aggtctactg ccactgcgat tcgtgccgta cctggctcag cgcgccgatc
     2641 cacggcgcca cgttgtggcc gaccaccaac gtgaaaatca cgaagggcgc cgacaatctc
     2701 ggcatgtaca agcgcacgga cgccagtcat cgccagttct gcaagtcctg tggcgccgcc
     2761 gtactggtcg gccaccccgc catcggcatg accgacgtgc cgtcggtgtc gatcaagggc
     2821 ctggcattct ccccggcgct gcacgtcaat tacagcgaga aagtcatggc cgtgcacgac
     2881 gggctgccga agttcaagga tttcccgaag gaattcggcg gctccggcga gatgcttccc
     2941 gagtagccgg atcccggtcc ggtccgacgc acggagaggt ccgccaggtg gcgactcgat
     3001 tcacggcgtg gctgtcaggg aagctcggac gcaacgagcc ggagcagccc aaagaggcgc
     3061 ctgccgcccg aaaatccgcc gagccctacc acgccgtgac catcaaggcc ggtgcggaga
     3121 gctgtgaagg cgccaagcaa ctcgccggcg tgcggttcct gtcgaccagc gcgcctcggc
     3181 tgccattgcc gaactgctcg cagccggcct gtttgtgcca ctacatccac cacgcggatc
     3241 gccgcaccgg cacggaccgg cgcgcggccg agaattggcg gcggacgcgc gattcgggcg
     3301 tcgagcggcg gcagcgcaag caagggcgcc gcgccaacga ttccatcgcg tgagtcggcg
     3361 acaaaaaaag aggccctcac taggagggcc aatcaggggg agaatgcgtg tagcgtaagt
     3421 gtgcaccgca caaattggga tacgtaaaga ccgagagcgg tgagacgtca gtcgatcccg
     3481 acgatttccg cctgtccttg ccggaacgaa acgcggtcgc ccggtttgct cccgatgagc
     3541 agttcggcca gcggcgagac gtaggaaatc agcccctgcg aggggtcgga ttcgtcttcc
     3601 ccgacgatcc gaaacgagac gcgttcgccg gtgtccgtct ccagttccac ccggcaaccg
     3661 aaccgcacgc gctcaggcct aggggtccgc gggaccagtt ccgccgtggc cttgcgacga
     3721 gtccagtacc gcaggtcgcg ctcgacgcgg gcgacggcag ccctgtcatc cgctgcccgc
     3781 gcctccgcca gctgcagacc caggccgcgg accatttgct cgattcggat gagcccctcg
     3841 ggagtgacga ggttgcgatg cctgctgacg ggcctttcgg gcaggtcgtc ggattcggct
     3901 ccgtcctgtt ccttgacgaa ggctcggctc atggctcgga tggatccgcc tagggctggt
     3961 cgggctcgac cggctccgac cgggacgccg gcaactgcag ttcaccctgc gacttgaacc
     4021 ggtaggtcga gagcccgctg ccgctcagct ttccctcgag cgagaagcgg atcggaccac
     4081 tggtcgttcc gccggccgat cccacgacgt gccccaccag gcgcaggacg gacaccgtga
     4141 ccggtatcga aatcaccgac tcgccgaagc gtggaatgtg accggttgcg tcgctcacgc
     4201 ctgtcgcaaa cgtcctgtcc tgcaccgaca gggccacgta agcgccgtcg tagtcgacct
     4261 cggcatcgtt cgggttctgc acgcgcagct tgagcgccat gcgcacctcg aggccttccc
     4321 cagggagcga gtcgatgccg gccaccgaga cctgcaacgg atcgccgtgg ggcaagccgg
     4381 cgcacgacgc gagcgcgaga gatacggtcg cgagccatcc tcgcagcagg gccgccctca
     4441 tagggggtcc gtcagggcgt ccgccggctt gggcgggtgc gtgcttccat gtccacgtag
     4501 acgagcatgc cgcacttcgg gcacttggcg gtgtcgatct cctcgacctc gaaccagcgg
     4561 cggccgcact cgcagcgaac ctggaggatc cactgggcga cggcacggcc gaagcgccgc
     4621 tcctcgacac ggaccccctc cggcatgcgc gtgatccggg ggacgggttc cgatcgggcc
     4681 ggctcgggtt ccccggcttc cgagacgtgg ctggtctcgc cgaggaagcg cagcaggctg
     4741 gccggactga ccagttccgc ctccacggga tcttccagtt cctcgatgac gggccaggcg
     4801 tcctcgcgcc ctggtccctt gcctgcgccg ccgatcatgt gccccacctc gatgccgacg
     4861 tcccggcaac cctaacgata ctactccgcg cggccggcgt caggccttgc cggcgaggtg
     4921 cgcccgtacg gtgtcgttta taagccgcag ttcggccagg gtcgtctcga tgtgggcacg
     4981 gcgctgctcg agttcctcga tggcggtgtc ggtgcggctc aggacgtatt tcatctgctg
     5041 gacccgccct tcgccccggg ccccgtacag gtcgaggtag tgcttggtct cggcgagcgt
     5101 cgacccgatc gccttggcgc gcaggatcag ggccagccgc gcccgatccc gccgcgtata
     5161 gacccgcgcc ccgttcacgc gccgggggct aagcaggccc ttgtcctcgt agaatcgtat
     5221 ggcgcgagcc gacacgccga attcccggac cagctgggtg atgccgaaga gctcggccgt
     5281 ggcgtcgtcg cggtgcgcgg ccagcacctc gcgcgcggtc cgggcgacgc cgcccggcgt
     5341 ccgcgcccgc ctgcgggacg gagcccgggc cgtcgcccgg gtcgctgtcc gccccgccgc
     5401 cacctcagac gacccgctgc agcttcatcg cgatgctcat gtcgcccgcg acggtgaact
     5461 tgcccgtcat gaagcccgtg atcgagtcga gctggccggt gagcagcgcc tcgagattct
     5521 cgcgggtgat gctgatcgtg cagtcggcat cgcggttctc gttgctgacg gtgttaggca
     5581 ccgacttgcc gtcgatcacg atgacgccgt cggcgccgca gtcgaacttg agcaccgcgt
     5641 cgaggccgct ggcctcgccg accttgccgc ggatcgtctc ggtgcactgc tgcatgtcca
     5701 tgatggaatt cctccggtgg gtgatggcgc aggggcggca tcttagcatc ggcgggtcac
     5761 cgatagggaa gcccccgcac agtgggaaag cctgttgacg tatacgtcat cccgatgcct
     5821 aatacgggtc cacccggccc cgctccgccg ccagcacatg agccacgcgc ccgacgcccc
     5881 ggatcccgaa ccgacccccc gttaccggag cgtgttccgc ccgggactgt tcgccggccg
     5941 cattgcctgg gtgacgggcg ggggcagcgg catcggccgc tgcgtcgcgc acgaactcgc
     6001 gtcactcggc gcactggtcg tgatcagcgg ccgcacggcg tccaggctgg acggcgtggc
     6061 cgcggagatc gccgccgacg gcgggcgctg cgagacgatc gccttcgaca tccgcgacga
     6121 ggaagcggtc aaggcgtcgg tcgccgcgac cgtgcagcgg cacggccgcg tcgacgcgct
     6181 ggtgaacaac gccggcggcc agttcccttc ccccctcgtc gcgatcagca agcggggctt
     6241 cgacgcggtg gtcgcgaaca acctcaccgg cggcttcctg atgatgcgcg aggtgttcgg
     6301 gcagtcgatg cagaagcacg gcggcgcaat cgtcaacatg accgcggact tccgcaatgg
     6361 catgcccggc atgggtcact ccggggcggc ccgcgccggc atgagcaacc tgacgatgag
     6421 cgcggccttc gagtgggcgc atgcgggtgt ccgcgtgaat gcggtcgcgc cgggctgggt
     6481 cgcgtcgtcg ggcatggaca cctaccaggg cgcggtccgg gccatgatcc cgaagctcaa
     6541 ggcgcatgtg ccgctgcgtc gcctcgcgac ggaggcggag gtcagtgccg cgatcgtgtt
     6601 cctgctgtcc cccgcggccg cgttcgtcac cggggtcacg ctgcagatcg acggtggcgc
     6661 ctcgctcggc agcgccgtgt tcccgagcat cgatcacgac cgctcgcagc cgttcgacgg
     6721 cttccatcgc gcggtgaccc cggaggcgct gcgctgatgc ccgccatcgt ctcccagctc
     6781 gatccccgca gcgccacgtt caagtcgaat gcggaccgca tggcggcacg gctcgccgag
     6841 atccgggcgc tcgaggcccg agtcgtcgag gagtccgcct cgaagcgggc gaagttcgag
     6901 gagcgagggc aactgctgcc ccgcgagcgt gtcgcacgac tgctcgaccg cggcagcgat
     6961 tttcttgagt tgagcgcact ggccgggctc ggcatgcacg acgacgacgg ccgcaagagc
     7021 atccagggcg gcggctcgat cgtcggcatc ggtgtcgtgg ccggcaagcg ggtcgtggtc
     7081 tcggccagcg acagcgcggt caagggcggg accgtcgccc cgatgggcct gaagaaaggg
     7141 ctgcgggtcc aggccatcgc gctgcagaac aagctgccgc tcgtgtccct cgtggagtcc
     7201 ggcggggcca acctcatgta ccaggccgag atcttcgtgg acggcggccg cagcttcgcc
     7261 aaccaggcgc ggctctcggc cgccgggatc ccgcaggtcg ccgtcgtgca cggttcatcc
     7321 accgcgggcg gcgcgtacct gccgggattg tccgattacg tcgtgctggt gaggaaccgc
     7381 tcgagcatct acctggccgg gccgccgctg gtgaaggcag cgatcggcga ggacgcgacg
     7441 gacgaggcac tcggcggggc cgagctgcat gccacggtca cggggctcgg cgagtacctc
     7501 gccgaggacg acgcgcacgc gatcgcgatt gcgcgtgaac tcgtggcggc gctgcgttgg
     7561 gacgaagtcg cagcacgccc gtcatccgtt ctcccgcgct acagttcgga agaactcatg
     7621 gggatcgtcc cgaccgacga gcgcacgccg tacgatgcgc gcgaggtgat cgcgcgcatc
     7681 gtggacgcgt cggacttcct cgaattcaag gcgccctact cgacggacac cgtctgcggg
     7741 cacgcgcgga tcgacgggca ccgcgtcggc atcatcgcca acaacggccc gatccagccg
     7801 accggctcgg tcaaggccgc gcagttcatg caactgtgcg accagacggg cacgccgctg
     7861 gtattcctgc agaacacgac cggttacatg gtgggcaccg ccgccgagcg cgccggcgcc
     7921 atcaagcatg gctccaagat gatccaggcc gtcgccaacg cgcgggtgcc gaagttcacc
     7981 attgtgctcg gcggctccta cggcgcaggc aattacggca tgtgcggacg cggcttcgat
     8041 ccgcgcttca tctttgcctg gcccaccgcg cgcaccgccg tgatgggtgg cgcgcaggcc
     8101 gcgatggtca tggagatggt cagccgcggc aagctcgcca agtccggcgt ccccgtgaac
     8161 gagcaggccg aggcgggatt gaaagccatg agcgaccagc tcaagcgccg tctcgacgcc
     8221 gagagcgacg tcctctatgg cacggccagg ctctgggacg acggcgtgat cgacccacgc
     8281 gacacgcgcc gcgtcctcgc actgtgcctc gccatcgcca gcgaggcgga cgcccgcacg
     8341 ctccacccga atagtttcgg cgtcgcacgc atgtaactga ccctcttccc gccgctcccc
     8401 ggagttccgc atgcagttca ccgaccagca ccgccagctc gacgacacgg tcacacggtt
     8461 cgtcgcgaac gagatcaatc cgcacatccc tgagtgggaa aaggcgggcg agttccccag
     8521 tcacgagatc ttcaagaaac tcggtgacct cgggctcctc gggctcaagt atccggagga
     8581 atacggcgga ctcggcctcg acttcagcta ttcgatggtg atggccgagg cactcggggc
     8641 ggctgactgc ggcggcgtgc ccatggccat cggggtgcac accgatatgt gcacgcccgc
     8701 cctcgcccgc ttcggcaccg accagctgcg gcgggaatac ctcgccccgt cgatcgccgg
     8761 cgacgtcgtg ggatgcctcg gtatcagcga accgtcgggc ggctcggacg tggcggccgt
     8821 gaagacgacc gctcgcaagg acggcgacga ttacgtcgtg aacggcacca agatgtggat
     8881 caccaacggc atgaaggccg actggtgctg cctgctcgcc aacacgagcg acggctctcc
     8941 ccaccggaac aagagcctga tcatcgtgcc gatggacgca aagggcgtca cgcgccagaa
     9001 gatccacaag atcggcatgc acagctccga cacggcccag ctctttttcg acgacgtgcg
     9061 ggttccccgc cgcaacctga tcggccagga aggcatgggc ttcacgttcc agatgctgca
     9121 gttccaggag gagcggctgt ggggcgccgc gagctcgctg cgcagcatgg accgcatcat
     9181 cgacctcacg atcgaatacg cgcgcgagcg caaggccttc ggccggagca tcctcgacaa
     9241 ccaggtcgtg cacttccggc tcgccgagct gcgcaccgag atcgcagcgc tgcgcgccct
     9301 cacctaccag gcggtcgagc agtacgtcgc gggcaaggac gtgaccaagc tcgcctccat
     9361 ggcgaaactc aagtgcgggc ggctgtcccg cgaagtcgcg gactcgtgcc tgcagtactg
     9421 gggcgggatg ggctacacgg cggagaacct cgtcgcccgc tcataccggg attcgcgtct
     9481 cgtctcgatc ggtggcggcg ccgacgaggt catgctcggc atcatcgcca agctcgaagg
     9541 cacgctgccg cgcgccgcgt cctgaccgtg aacgacgcga gcaccctcgc catccgacgt
     9601 gcgggcgccg tcctgcacgt cgcgctcaac cgtcccgagc tgcgcaatgc gatgtcgctc
     9661 gccatggtcc gggaactcac gaccacgctg cacgcggccg agagcgacgg cgagacacgc
     9721 gtgatcgtgc tgcgcggtgc gggcggccat ttctgcgccg gtgccgacct caaggacatg
     9781 gcgggtgcgc gggcgcggct gacgcaggac cccgacgcca tcctcaaggt gaatgcggca
     9841 tttggggcgc tctgcaacgc gtacgccgca tccgggctgg cgatcgtcgc cgtgctcgaa
     9901 ggcgccgtga tgggtggcgg attcggcctc acgtgcgtgg ccgacgtggt gatcgcggga
     9961 gacagcaccg tgttccggct gcccgagacg tcgctcggcg tgctgccggc gcagatcgca
    10021 ccgttcctcg tggagcggct gggctactcc gaggctaagc gcctcgccgt caccggcagt
    10081 cggctcgatg cgcgcgaggc gctcgcgatc cggctggtgc acgaggtgca cgctgcgcag
    10141 gacctcgacg cggcgctcgc gcgccagctg caggacatcc tggcgtgcgc gccacaagcc
    10201 ctggcagcga ccaaggccct gctccgccgg gcccgctgga caccaggggc cgacctcgtc
    10261 caggaagccg cggcggcatt ctcgcgggcg gcactcggcc ccgaaggcac cgagggcacg
    10321 accgcgttcc tgcagaagcg caagccgaac tgggccccgc agtgaccttc cggaggatac
    10381 tcatcgcgaa tcgcggcgag atagcctgcc gcgtcgtgcg taccgcacga atgctcgggt
    10441 accgcaccgt cgccgtttac agnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nngccatggg cgacaaggcg ggcgccaagc ggcgcatgct cgccgccggc gtgccttgtg
    10621 cacccggcta cctgggggac gaccaggacg acggacgcct gctcgcggag ggcgcgaagc
    10681 tgggcgtccc gttgctcgtc aaggcgatcg ccggcggcgg tggccggggc atgcgcctgg
    10741 tgcgatccct cgacgagttg cctgcggccg tcgccggtgc acgccgggag gcactgagcg
    10801 cattcggcga tggcgcgctg atgctcgagc gcctgatcga gggcgggcgc cacatcgaga
    10861 tccaggtgtt cgcggaccgt cacggcaacg cgattcatct cggcgagcgc gactgcacca
    10921 cgcagcgtcg gcgccagaag gtcgtcgagg aggcaccctc tcccatcgtc gatgcggcga
    10981 tgcgtgcccg catgggagca gacgcggtgg ctgcggcgcg cgccgtcggt tacgtcgggg
    11041 ccgggaccgt cgaattcatc gtcgacgcgc ggctccagca ttacttcctg gaaatgaaca
    11101 cgcggctgca ggtcgagcac ccggtcaccg agtcgatctg cggactcgac ctcgtcgagt
    11161 ggcaactgcg cgtcgccgcg ggcgagcctc tgccgctggc acaggaggac gtgcggttca
    11221 cagggcacgc gatcgaggca cgactctatg ccgaggatcc gtacgccggg tttgcgccgc
    11281 agaccggccg catcctgcat tggcggcccg agcgcgcgga agcgcgcgct gtgcgcgtcg
    11341 attcgggcat cgcggagggc gggacggtgt cgccctacta cgacgccatg gtggccaagg
    11401 tcgtggcgca cggccgcgat cgcgccgacg ccgcgcgtcg tctcgcgacc gccgtcgagg
    11461 acgccgtgct ggtcggagtg aagaacaacg ggcgattcct gcgcgacctg ctgtcgcacc
    11521 cggacttcac ggcgtcccgg atggacacgg ggacgctgga tcgctggtcg gaatcgggcg
    11581 aacccgtgct gcaggcaccc gcgataccgg agaccgtgtg gcgactcgcg gccgcggtgc
    11641 tcgcaacgcg caatggcacg ggctggcgcc cggccagcgt cgcgtcctac gacctcgagc
    11701 tgatcagcgg cgacgagcgg cgcaacctgc gggtgcgcgc gcaggcacgc agcgtggacg
    11761 tgacgagcca cgatgcaacc tcgagtatcg agatcctgtc ctggcgcgac cacgacctgc
    11821 ggtatgccga gggcggccga atgcacacga ccgtgtgcgt gctggaggac gacgcgctgc
    11881 acatcgccag cgacggcgcc gtattcacga ttcgcgaggt gtctccctgg ccgtcccgcg
    11941 acgcgaacgc ggacccacgc tatgcccgcg caccggtggc cggagcggtt gccgccatca
    12001 gcgttgcccc cggcgatcgt gtggaatcgg ggcagccgct cgtctgcgtc gaggcgatga
    12061 aaatggagat gtggctcacg gcgcgcagcg cgggcacggt gcgtgcggtg cacgtcgaga
    12121 tcaagcagag cgtcgaggcc ggggtgctcc tggtcgagat cgagacggaa ggaacggagt
    12181 gatggacaag gcgatcctga cctgcgcgct gaccggcgtg ctcaccaacc ccaagcagca
    12241 cccggtgccg gtgacgccgg cgcagatggc ggccgaggcg cgcgacgcgt tcaatgccgg
    12301 cgcgagcgtc atgcacgtgc acctgcggcg gcaggaagac ggcatgggtc acctgccgag
    12361 ctgggatccc gaggtcgcgg cggccgtcat cgacgcgata cgcgcggcgt gccccggcgt
    12421 gatcatcaac ctgaccaccg gcgtgatcgg caaggacatc tcgggcccgc tcgcctgtct
    12481 ccggcgcgtc aggccggagg ccgccgcctg caatgcgggc agcctgaact acctcaagat
    12541 caaggacgac ggcggctggg cgtggccgcc catggtcttc gacaacccgg tggagaagat
    12601 ccagcagtac ctcggcgtga tgaacgagct gggcattcac ccggagttcg agtgcttcga
    12661 cgtgggcatc gtccggtcgg tgggcatgta cctgaaggcc ggcatgttct ccggcgtgcc
    12721 cgaggtgaac ttcgtcatgg gcgtggcgag tggcatgccc tgcgatgcgg acctgctggc
    12781 gctgctgccg ggatacgcac cgcccggcgc cgtctggcag gccacgctga tcggccgcgg
    12841 cgagatctgg ccggtccacc agaaggcggc cgacctcggg ggcatgctgc gcaccgggct
    12901 cgaggacacg ttctacctgc ccggtggcga gcgggcgtcg ggaaacggcg tcctgatcga
    12961 ggctctcgcg cgctgcgccc gcaatgcagg ccgggaaatc gccacgccct cggaggcccg
    13021 cgtcctgctc ggggtgcatc cgtccggctg agccccggga ccgcctattg ccagacgacc
    13081 ggccggcgct tgccgaccca gctctcgtag tgcgtggcgt acaccgcctc gatgcggttg
    13141 cgcttcacct tcagcgtcgg cgtgacgagg tcgttctcca cggtccacgt gtcgcgcacc
    13201 accacgacgc agtcgagctg ctcgtgtgga tcgagcaatg cgttcaccgc gtcgaggtgc
    13261 gctgacagcg actgctcgag gtcgtcgcgg tgcgcgggct ccgcaatccg ctgcgaggtc
    13321 tcggcattca gcatcagcag ggccagtggc tggccgaggt tcgcgccggt cacgcagcag
    13381 gcctcgaccg ccacgtgagt caccaggcgg tcctcgatcg gcgcgggcgc gacgtacttc
    13441 cccttgcctg tcttgaacag gtccttcacg cgaccgacga tccgcaggtt gccttccgcg
    13501 tcgaactcac ccttgtcgcc ggtgcgcagc cagccgtccc cggtcatggc ctggcgggtc
    13561 aactccggct ccttgtagta gccgagcatc acgcccgggc atttgacctg caactcgccc
    13621 gtctcggggt cgacacggga ttgaatgccg tcgtaacccg ggccgaccgt cccgggccgc
    13681 ggcacgtccg gaagcgtcgc gtgcgagacg ccgcagttct cggtcatgcc gtagacctcc
    13741 gacagccgca gcccgagcct gcgataccac ttgagcagtt ccgccggcat cggcgccgcc
    13801 ccgccggcag cgaactcgca ctggtcgagg ccgagcgcgc ggaggattcg ccgcgcgacg
    13861 atgcggcgca ccaccggaat gcgcagcagc cggtcgagct tcgcggccgg catcttcgca
    13921 agcacgccct gctggaactt cacccacagg cgaggcaccg agaagaagat cgtcggcctg
    13981 gcacgccgga tgtccgccgc gaacgtctcg agcgactcgg cgaagtacac gtgcaggccg
    14041 aggccgagca ttccgtgctc gaccaccgtg cgctccgcca cgtgggccag cggcaggtaa
    14101 ctcaggacgc ggccgttctc gtgggcgccg atgcgccgca ggaccgtgcc gatggaccac
    14161 gcgaaggtcg cgaagctgtg catgacaccc ttcggcgtgc cggtcgtgcc cgaggtgtag
    14221 atgatcgtcg cgagctcgtt gccgtcccgg accggctcgc cctgcagtgg ctgcgtcgac
    14281 gtgacgattt cgtcccatgc cgggcagtcg accggcggcg acaacgggaa ccggatcatc
    14341 ggcagccctg tgggtacgcc gtcccgcatg gcgtcccagc cgtccagctt gccgacgaag
    14401 agcaggcgcg cctcgctgtg cgtgaggatc tgccggatgg ttccggccgc gaggctcgga
    14461 tacagcggca ctgacacgta gccggccatc cagatggcga agtccgccat gagccagtgc
    14521 gccgtgttct tggagagcag cccgatgcgc gcaccaggct cgaatccaag gctcttgaga
    14581 tgcgccgcca tgcgtcgcgt ctcgtccatc atctcgcgcc acgtgaggtc gcgcacgatg
    14641 ccgccgccca tgggctgcgt gagcgcgacg cgatcgggga tggtccgctc ccagcggtag
    14701 agccgctgca gttgcaggtc gtcgggtcgg acttcgctgt tcatcgatta cacccggtag
    14761 aaattgacta aaccgtcagc ccgggcccgc tcggggccgg gccgagagcg ttctactgca
    14821 cgtagatctt cacgacctcg tacaggaaac gacggccctc catcagcgac tgcacgcgca
    14881 tgcgctcgtt cagtccgtgt gcacggctgc cctccgcgtc gctgaacatc ccggacatgc
    14941 cgtacgtcgg aatgccggcg ccgttcagga atcgtccgtc ggtcgccccc gtcgacatcg
    15001 tggggatcac cggaacgcct ggccagatcc cggccgccac cttgcgcacc gggtccatga
    15061 tggcgggcgt gagcggcggc acgggggcca tgacgcccgg atcgccgacg aggctcacct
    15121 tgatgcccgg gtcggccacg acccgctcca gctcggcctt gaccgactcg atgggcacgc
    15181 ccggcaggat gcgacaattg acgttggccc gagcgcgctg cggcaacgcg ttcggcgcgt
    15241 gcccgccctc gatctcggtc gccacgcacg tcgtgcgcag catgccgttc cagctcgggt
    15301 tggcatccca gagcttgccg gcagccgccg catcggccgg gtttgcgacg atggctcgca
    15361 tggcgccggc aacgtcgggg ggctggagct gcgcctgggc ctggaaatag gctcgggtcg
    15421 tgtcgttgag ttggaccggg aactggtagg cggcgagtcg cgtgagcccg gccgccagct
    15481 cgttgatcgc gttcacgcgc atcggtcgcg aactgtgccc gcctggattc gtggtctcga
    15541 gcatgaagtc ctggtaggtc ttctcgcctg cctgtacgtc cagcgcgacc ggcttgccgt
    15601 ccgggccgag cagcccgccg gcgccttcgt tgagaacgaa cgccgcgcgc agcacgtccg
    15661 gccgcgtctg catgagccag tggacgctgt tgaacgtgtc gggtgtttcc tcgccgcagg
    15721 tgagtgccag cttcacgccg cgcttcggct tgaacccttg cgcctggaac cgcaccagga
    15781 tgtcggtgaa cacggcggcc atggccttgt cgtcgctcga gccgcgcgca tagaagtacc
    15841 cgccctcctc gaccagcttg aacgggtcac gcgaccagtc ctcgcgccgc gcctcgacca
    15901 cgtcgatgtg cgccagcagc atgaccggtg ccagcgacgg gtcctggccc ggcagggtcg
    15961 cgatcaacgc accgctcttc ggccggtcgg ggggtgcaag gatctccatc gagtccttcg
    16021 gcaggccggc tgcccgcagg cgtgcggcca tggcctcggc cgcctgcgtg caactgccgg
    16081 acgacagggt cgtattgatc tcgacgagtt ccttgtagat cgagcgaaag gtcgcctcac
    16141 cgtcgtcgct cgccacggcg gccgcgcaca ccatcgacga gtatgcacag cccgcgcacg
    16201 ccaccagtcc ggccaggcgg ccggcgattc tcggtccggt catttctcac ccccgtgaac
    16261 aggtctgcag gctagccgtg tcgcgccgcg acatgcaagc ctgtcagccg cggcggcgcc
    16321 tacacgaacc gctccacgag tgcgtgcacg ttgaccctgg cgcgcagttt cgcgcagatc
    16381 aggaatgtcc cgagcagctt gcggtgcagg aagatggtct gcggcggcgg cgaccgcagc
    16441 ccctggtcga acgcgacggc gaggccgagg tcccgcaccc gaccgatcat ccccgattcc
    16501 gcgaagtcgt acggcccgcg gtgcgccagc ggctcgcaga ccaggcggat gatgtccacg
    16561 acgccgcgcg cccgctccgc tgcgtcgtcg gggtgcaggt agccgatcgc cgcagcggcg
    16621 tcacggatcg ccacccggtc gtcggcgacc acggcgcggc tgatgcgccg gtagttgtcg
    16681 gtgaattcgc gcgagaactc gcccaccgag ccgaggtcca gcagcccgat ctgctgcgtc
    16741 gccggcaagt acaggtaatt ggcgaagttc gggtcggtct gcatgaaccg gaactcgaac
    16801 agctcgcgga acatgagttc ctggaggacc ttcgccacgg cgttgcgcgt tgcctggggc
    16861 acggcctcgc tcgccagccc ctccagcggc tcgccgtcca tccagtccat ggcgaggatg
    16921 cgccgggtcg tgagcccccg gtggatgcgc gggacgacca cctcgggcat gtcagcgacc
    16981 agccgcgcgt aacgctcgag gttccgagcc tcgccttcgt agtcggtctc gagccggagc
    17041 tggcgcttgg cctcggcggc gatcgcaccg atgtcgatgg tcaccggcag aaagtccagg
    17101 cgccgcagca gggtcgcgag gttgtccacg tcgctgtcga cgctgcgggc cacgccggga
    17161 aactggacct tgagcgccag ctcgcggccg ccatgcgtcc gggcacgatg tatctggccg
    17221 atggatgcgg cagcggctgg ttccatgtcg aatcttgcaa acttcgcctc ccacccgcga
    17281 ccgtactcgc ggccgagcac gcgccgcagc tgcgagtccg gcatggcgta gccctgcgag
    17341 cgcagcatcg agagcgcatt gcggaactcg gccgggatga tctcgtcgct ctgcagcgac
    17401 agcatctggc caaccttcat cgccggaccg cgcagtgccg ccagcctgcg cgcgagcagt
    17461 cccgcgttgc ccggcgtgag cacggccgca gccaggtccg ggcgctttcc gctcgtgagc
    17521 tgtcgcgcgg ttgccgcagc ggccgagagc gcgagttcgc cgctcaacag gccgaaccgc
    17581 agcaggcgcg aggcccgcga ggccggcacc gcgctgcgct tgcgtttggg gtcgtccgac
    17641 atcgtgtgct ggcgccggtg gtcgtcaggc caccgcagac tcttgcgtca cgccgcgtgc
    17701 tgcgcaggcg gcacgacgga ccacgacgag tacatgtgca gccggcccgt gcgcacgtcg
    17761 cacagcgccc gccgctgctg cccttcggcc atccattcct tgatggcgac ccgcgccggc
    17821 agctgtacgg gggtcaggaa ctgcgtctcg atcgtcgtcc cggccgcgag cgccggcacc
    17881 ggcggctggg cgaggctgcg cgccagcgac cacatgccgt ggctgatcgc gccgcgcagg
    17941 ccgaggtacc gcgcgatccg gtcgctcagg tggatcgggt tgtagtccgt ggacacgcgc
    18001 gcgtagtccc acgccgtccg gctcgtcacg tcgagttccg cgagcacggc ggcatccttc
    18061 ggcgccttgg gcggtcgcgg ggggcgaacg ccgccgcgtt cggcggactc cggccagcgc
    18121 gacaggaaca cgcaggtctc cagccagacg ggccgccctt cgtggctgat ctcggtcgcc
    18181 atgtcgaaag cgagccccgc gtcggtgcgc cggtaattgc gcgccgacac gctgaagtcg
    18241 agtcgcatgc cgtaacgcag cgcggaccgg atctcgatga cgttagacag gtggatcagc
    18301 cccatcggcc gcagcggaaa cgacgcgcag gcgaagatct cgaggtgcag cggcatcgcg
    18361 agcacgtgca ggtacgtcgg cggaatgcag tcgccgtagc cgcacacctt cgcatacttc
    18421 gccacgctga cccgcgagag cgccacaccg ctgacgttcg cggtgaacac cggcggatca
    18481 acgacctcgg tgatcgcggg ccgcttcgtc aacaccacgc gcggatacgt cgcaagcccc
    18541 gtcggcagct tgcgcaggcg caagtggacc gacgcggcca cggtcaggga gcgacctggt
    18601 tgatcacgat cgcgacgtcg gtcgggccgc ctttggagtt cttcacctgg ccgacgaggt
    18661 cgccggtcgc aacggtggga gtcccgccga tcgcgacgtg cgcgacgatc tccacggtat
    18721 ccaccgacga cagattgcgg ccctcgacca tcgaattcgc gtcgctcagc acgacggtgg
    18781 tcggcaactg gtcgctcgac agcctgacgg ccgccagcgg cggcccgggg attccgggct
    18841 cccgggctgc cacgaacaat ggcatacccg gcttgagctt cgctgccacg gacggatcca
    18901 cggatatcga gacccgcaag gtgcgtccgg cgggtgtcac gttcccctgc ccttggcccg
    18961 actgcgcgtc ggccgagcct gccatggcgg acgcgccctc gccctcagcc gccgggactt
    19021 gcacgccgag gcccgcgagc tgctcgacga cgatctggcg gatctgcggt ggggggttct
    19081 gctcgagcag cttctggaat gccgctttcg cggtgtcctt gtcgccgcgg cgcatggcca
    19141 tcacgcccga gtaccagagc gccttgccgt tgttcgggtc ggcgacgagg gtcgcgtcga
    19201 tgatgtcctt cgcacgcgcc tgcagcgaag gatcgtcggt cagcacnnnn nnnnnnnnnn
    19261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19321 nnnnnnnnnn nnnnnnnnnn nnnnnntcgc gtcgatgatg tccttcgcac gcgcctgcag
    19381 cgaaggatcg tcggtcagca cgagtgcctc ggcgaggtcg agcttgagcc cgggatccgt
    19441 tcccccgacg atggcatccg ccttttcata ggcggtgacc gccttgccgg cctggccggt
    19501 cacgagataa ctccgaccca gcatctgcca gccctcggcg tcctgcgggt tggcggccat
    19561 gcgcgcctcg agctgcttga ccgcgtcggc cagtgagcca gcctctcctt cgccatggcc
    19621 agccggcacg gcgctcgcgg cctcgggatt cttccaggga aagttggtga tgccccaata
    19681 caaggcgccc gccgcgagcg ggagggtgat cgcgacgacc gcgaatgccg acgtcgagcg
    19741 tggcgacgcg ggttcgccct tcgccgcgac cggctgcttg cgcgccagcg gtacggcgac
    19801 gagcgctacc gcggcgcaga ccatgacgat gcaggctacg atgaatgctg tcacgactgg
    19861 ccgtcctcct ggccttcggg atcggtgtct gcctcggtcg aacgacgacg gatgacgcgc
    19921 accacggcca acgcgccgag cgcgaccagc aggaccggcg ccgaccacaa caggaacgtg
    19981 agcggtgtca tgcgcggccg gtacaggacg aagtcgccat atcggtccag catgaactgg
    20041 cggatctggt cgtcggtctt gccggccgcg atcatctcgc gcacttcgcg tcgcaggtcg
    20101 ccggcgagcg tggcgttcga atcggcgatc gtctcgttct ggcagaccag gcagcgcaat
    20161 tcgcgggtga tgttctcgta acgcgcctgc agcgccgggt cctcgaaggc gcgctcggta
    20221 tcgatcgcat gggccgttcc cgacgctgcg aacgcgatcg ccagcagcag tgcacgcagc
    20281 atcttcgtca cgtcgcgcct cccttaccgg ccttggccgc ctcgatgcgc ggcaggaact
    20341 cgcgaaccca cacttccatg gtcatcgggg cgatgtgctt gtacagcacc acgccgtccg
    20401 gaccgatcag gaacgtctcg ggggcgccgt acacgcccca gtcgatcgcg actcgaccct
    20461 cctggtcgga ggccacggcc gagtacggat tgccgagctc gctcagccat tgccgggccg
    20521 ccacgctgtc gtccttccag tcgagcccga tcatcgggac cgccttctgc cgcgagatct
    20581 ccagcagcac cgggtgctcc tgccggcagc cgccgcacca cgtgccccag acattcagga
    20641 cccaggtctg gcccttgagg tcgccgctcg agaccgggta ggacgcatcc tgcaggctgg
    20701 gcagggagaa cgcaggggcc tgcttgccga tcagcggcga cgggatgtaa gacggatcgc
    20761 ggttcaggcc gacgagcagg aacccgccga tcacgacgaa gatccccagc ggaatgatga
    20821 atttccacat gcgccgctgc ctcgtcaccg cgcccgggcc ggggcgcccg cgcccacctt
    20881 ctcggtcgcc gcttcccgcc gcgtgcggta gcggcggtcg gtgatgccga tcagtccgcc
    20941 gagcgccatg acgatggcgc ccagccagat gaaccggacc atcggcttgt actggacgcg
    21001 catgctccac gccccggcgc cgaggtcctc gcccatggcc acgaacaggt cgccgttcca
    21061 gttcacgtcg atccccgcct cggtcatcgg gctgcgctgc acgcggtaca cgcgcttctg
    21121 cggatgcagc accttcacgg tccggccgtc gcgcttgatc gcgacctcgg cctcgatcgc
    21181 ctggtaattc ggtcccggca cctcgcgggt gctcacgaac tggaactcgt agccgtggat
    21241 gctcacggac tgccccggct tgagcgcgat gtccttctcg atgcggtagt tctgcgtgat
    21301 ggtcacgccc agcgcgaaca ggccgacccc gatgtgcgcc agcgtcatgc cgaccatccc
    21361 gcgcgagatg gtctgcttct tgcgccagcg ctgcaccggc tcgtagagcg acgacaggat
    21421 cacccaggtg ccggccgcga tcccgacggc gctcatcacc gtgaagtcgc ggtatgccgc
    21481 caccgtgacg agcagcgcga atgcgaccga ggctcccagc atcacgagca gcggcttgcg
    21541 catgtccgcg agccggccct tcttccaggc cgcgtgcatg cccaccgcga gcaggaccat
    21601 gagcggcacc atcgggatca ggaacacgac gttgaagtac ggcggcccga ccgagatctt
    21661 gccgaggttg agcgcgtcga ggaacaacgg gtacagcgtg ccgatcagga tcagcgccga
    21721 ggcgacgacc agcagcatgt tgttgaacag gatgaacgac tcgcgtgaca gcatctcgaa
    21781 accgcccttg gcgcgcagcg ccggaccgcg ccacgcgaac agcgcgagcg atccgccgat
    21841 cacgaggccg aggaacgcga ggatgaacag cccgcgcttc ggatccgacg cgaacgaatg
    21901 caccgacacc agcacgccgg agcgcaccag gaacgtcccg accaggctca gggagaacgc
    21961 gatgatggcg agcagcagcg tccagctctt gaagagcccg cgcttctcgg tgaccgcgag
    22021 cgagtggatg agcgccgtgc cgaccagcca cggcatgaac gacgcgttct cgaccgggtc
    22081 ccagaaccac cagccgcccc agccgagctc gtagtacgcc caccagctgc cgcccgcgat
    22141 ccccatcgtg aggaacagcc acgaaatcgt gg
//
LOCUS       DAOMFD010000014        21679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_422_length_21679_cov_36.367231, whole genome shotgun sequence.
ACCESSION   DAOMFD010000014 DAOMFD010000000
VERSION     DAOMFD010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 21679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21679
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_422_length_21679_cov_36.367231"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1174)
                     /locus_tag="PL152_01740"
     CDS             complement(<1..1174)
                     /locus_tag="PL152_01740"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQR48024.1"
                     /translation="MPFTLKDPTLLRQQCYVDGTWIDADGGGKIDVTNPATGEVIGTV
                     PNLQAAETRRAIEAAAAAFPAWAAKTAKERSVILRRWNDLMLANADDLAVIMTAEQGK
                     PLAEAKGEVAYAASFIEWFAEEAKRVYGDVIPGHQPDKRIVVLRQPVGVVAAITPWNF
                     PAAMITRKAGPALAAGCTFVCKPATQTPYSALAMAELAHRAGVPKGVFSVVTGSAAAI
                     GGEMTSNPIVRKLTFTGSTEIGKKLVAQCAGTLKKVSMELGGNAPFIVFDDADLDAAV
                     QGAIASKYRNTGQTCVCANRLLVQEGVYDAFVAKLVEAVRKLRVGDGLAGATEQGPLI
                     DAKAVAKVEEHIADALAKGGKVALGGKRHALGGTFFEPTIVTHVTPDMMVAREETFG"
     gene            complement(1288..1653)
                     /locus_tag="PL152_01745"
     CDS             complement(1288..1653)
                     /locus_tag="PL152_01745"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HQR48025.1"
                     /translation="MAIDRSAARSLVAGAGALLLVVCGAARSDSMRCGSRIVKDGDTM
                     EKVLAVCGEPAIRSRTWIQRPPQFALGGWTYSFPGTEDVPVDSWTYDFGPNKLMQRVR
                     FVAGEVESIVTLGKGTSEY"
     gene            complement(1643..1984)
                     /locus_tag="PL152_01750"
     CDS             complement(1643..1984)
                     /locus_tag="PL152_01750"
                     /inference="COORDINATES: protein motif:HMM:NF022459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2845 domain-containing protein"
                     /protein_id="HQR48026.1"
                     /translation="MLALLAALAVGVFAAPTASADDTMRCGTRLVSEGDGKDKVRTLC
                     GEPTSISLAGYISGPLYEDGPYGYQYFWPGWTALPVEIWTYNFGPNKLIRTLRFVGDE
                     LDEIRTNGYGY"
     gene            2202..3779
                     /gene="gshA"
                     /locus_tag="PL152_01755"
     CDS             2202..3779
                     /gene="gshA"
                     /locus_tag="PL152_01755"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HQR48027.1"
                     /translation="MAPSRTFDRRLAALVNRQATRALQGGLKGVEKESLRVTPRGLIA
                     TTPHPRALGSALTNEHITTDYSEALIELVTPPFPQTWELVQYLCDLHQFVYRHLADEL
                     LWATSMPCAIKGEESIPIAEYGSSNVGRMKTVYRRGLGHRYGRVMQAISGVHFNYSFP
                     EHFWPVLGDLLESRDTGQAFQSDAYFALLRNYRRHGWIVLYLFGNSPAICPSFLQGRK
                     VDWLEPLEPGSLYAPHATSLRMSDLGYRNKSQAGMSVSVNSLEHYVRDLAQAISTPHA
                     EYEKLGVKVDGEYRQLNANLLQIENEYYSFIRPKRVTMSGERPTKALRRGGVQYVEMR
                     SLDVSAFDPVGVNQMKLRFLEAFAAYCVLAASPPIEPAEQAELDGNHALVAREGRKPG
                     LMLRRDGRDLSLRDWASEIVDSMRGVCELLDEGDPQRHHSAALEVQAAKVREVALTPS
                     ARSLEEMRTNEESFFNFALRMSKLHKSYFLELHPPHEARQQAFAAEAEESLQAQAKIE
                     ASDTIGFDQYLAQYFAG"
     gene            complement(3849..4760)
                     /locus_tag="PL152_01760"
     CDS             complement(3849..4760)
                     /locus_tag="PL152_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HQR48028.1"
                     /translation="MIAIAVHGGAGVMPGKDLTAARQQAFHDGLAAALRAGFAVMESG
                     GLSLDAVVAAVKVLEDDPLFNAGRGSVLAANGLHELDASIMDGRDLRAGSVSGVSHVR
                     NPVELARLVMDRSPHVMLAGAGADEFAIQQGLEPVPNEYFATERRRLELERHLHGEAD
                     VEDEAVLGTVGAVALDAHGNLAAATSTGGMTAKKWGRVGDSPIIGAGTYAANDCCAVS
                     ATGHGEYFIRAVVAHEIASLMRYRGMSVSEAADEVVMQQLVRLGGSGGVIAVGRDGSI
                     AMPFNSPGMLRGAMDSRGRFETGILRK"
     gene            4904..8371
                     /locus_tag="PL152_01765"
     CDS             4904..8371
                     /locus_tag="PL152_01765"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004736 - pyruvate carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HQR48029.1"
                     /translation="MRQPGIIRTLLVANRGEISIRVMRAANELGIRTVSIYSQEDRFS
                     LHRMKADESYLVGEGKGPVEAYLDIADIIRVAREAGADAIHPGYGFLSENPEFAEACA
                     AAGIIFIGPTAEIMRSLGNKVTARALAVSAGVPVMPATPPLPRDDAECARLAAGVGYP
                     VMLKASWGGGGRGMRVVEGEAQLAEMVATARREAKAAFGNDEVYLEKLVRRARHVEVQ
                     ILGDAHGNLVHLFERDCTVQRRNQKVVERAPAVFLTGTQRAELCELGLRIGRAVRYLN
                     AGTVEFLQDADTGQFYFIEVNPRIQVEHTVTEVVTGIDLVKAQIRIAEGAVLGQPGSG
                     VPSQPDIRMNGHAMQCRITTEDPENAFIPDYGRITAYRSPAGFGIRLDAGTAYTGAFI
                     TRSYDSLLVKVTAWAPTPDETIARMHRALWEFRVRGVVTNLRFLDQVITHPRFENAEY
                     TTRFIDETPELFRFPRKRDRATRLLRYIGDVIVNGSPEVIGRARPTRTLDPRPPRVDL
                     ATPPPPGTKQRLDELGPRGFAQWMLAQEPVLLTDTTFRDAHQSLLATRFRTRDMAAVA
                     PAYARLVPNLLSVECWGGATFDVAMRFLKECPWDRLSAFREAMPNLLLQMLLRSANAV
                     GYTNYPDNVVRYFVAQAAANGVDLFRVFDSLNWVENMRVAMDAVLETDRLLEAAICYT
                     GNLTDPRQTKYDLEYYVRLAKQLERAGAHVLGIKDMAGLCQPRAAYTLVKALKDEIGI
                     PVHFHTHDTSGIAAASVLMAVEAGVDAVDGALDAMSGLTSQPNLGSIVEALRHGPRDT
                     GLDPENLRLLSNYWEQVRHGYAAFESDMRAGASEVYVHGMPGGQYTNLREQARSLGID
                     DTRWHEVARAYAQVNEMFGDIVKVTPSSKVVGDMAIMMVTSGLSVGEVLDPDTEVAFP
                     ESVVSLFRGDIGQPQGGFPPALQKKVLKGATPLTVRPGAVMPPVDLARQREELQAKMP
                     RPISDREFASWLMYPKVFTDYMTERVQYGDTSVLPTRTFFYGLGAGEEITLDLERGKH
                     LIIRYVTTSEVHDDGTRTVFFELNGQPRSVKVTDRSKVAKRPPRRKAEADHPDQVGAP
                     MPGTIATVHAVAGHHVQRGDVLVTIEAMKMETSVRAERDGTVVEVVARPGEQVDAKDL
                     LVVLAESGK"
     gene            8368..9042
                     /locus_tag="PL152_01770"
     CDS             8368..9042
                     /locus_tag="PL152_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQR48030.1"
                     /translation="MTETDGSGLGAQLRELYPALAGLPERDIEILLSRCSVLSLRAGA
                     QLFGEGSACRQFPLVIEGSIRVAKCGEGRELQLYRVTPGECCVLTSSCLVGGRDYPAT
                     GVVESDVRLVVMPQAEFESLMARHPPFRNYVFGLFAERLADLMGLVEAVAFHRLDRRV
                     ASALLGHGPVVEMTHQQLADAVGSVREIVTRVLGSLADQGVIRLGRGSIEILDAVKLR
                     QVAEGA"
     gene            9108..9383
                     /locus_tag="PL152_01775"
     CDS             9108..9383
                     /locus_tag="PL152_01775"
                     /inference="COORDINATES: protein motif:HMM:NF022577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQR48031.1"
                     /translation="MQRPNSIDSGIRPQAGIETIRKEHSNMAANVGGVDRTLRIVVGI
                     VLIALYFVLSGPARYVGLLGLVALFTGLVRFCPLYTLLGINTCATRK"
     gene            complement(9373..9807)
                     /gene="trxC"
                     /locus_tag="PL152_01780"
     CDS             complement(9373..9807)
                     /gene="trxC"
                     /locus_tag="PL152_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006662 - glycerol ether metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HQR48032.1"
                     /translation="MSSRKVVCQKCNGVVGVPEGRLGDDPRCPRCHESLFSGHPVELD
                     SGNFDSHITRGDLPVVVDFWAPWCGPCRMMAPALEETARQMEPAVRFGKLNTDEAQDV
                     AARYGIRSIPTLIVFKDGREVARQSGAMGAQQLNRWVSAAIS"
     gene            9985..10938
                     /locus_tag="PL152_01785"
     CDS             9985..10938
                     /locus_tag="PL152_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQR48033.1"
                     /translation="MRYKDYYKVMGVARDASQEDIKRAYRKLARKYHPDVSKEKDAEE
                     KFKELQEAHEVLKDPEKRAAYDQLGADWRPGQDFRPPPDWGKGFEFSRGARGGGGEGL
                     GEFSDFFAELFGERSPFGRPGGGARAGHGFAAQGQDHLARVEIDLEDAYRGGTRDIEL
                     RAPEITPDGHVLTRPRTLRVTIPAGVTEGQQIRLAGQGSPGLGGGPPGNLLLEIGFRP
                     HARFKAEGRDITLTLPVAPWEAALGESVSVPTLGGPVDMKLPAGARAGQKLRLRGRGL
                     PGPACGDQYVILEIVLPPDSPKARRFFEQMKRELPFDPRAD"
     gene            complement(10992..12020)
                     /locus_tag="PL152_01790"
     CDS             complement(10992..12020)
                     /locus_tag="PL152_01790"
                     /inference="COORDINATES: protein motif:HMM:NF014090.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - cellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HQR48034.1"
                     /translation="MSGLESWREEKQSAWLYRALAECEPDARIATLFRTLASSAEGQA
                     DKWRAESGGEPPAFAPSVRARITASLARTLGPRRIRPVLVAMKVRGVSAYDARTATPG
                     HTMPTSVSQVGVRHKGYGGGNLRAAVFGVNDGLVSNASLIMGVAGAGAGIGGVVTSGV
                     AGLLAGALSMAAGEYVSMRSQREMFEYQIGLERDELHEYPEEEAEELALIYNTRGMEL
                     EEARRITRELVKNHDAALDALAREELGLNPDDLGSPWGAAIFSFLAFAAGASLPLLPF
                     LLGLPLERAVTVAAATAGIALFGVGAALSLFTGRNAWLGGLRMVAIGGGAGAVTWIIG
                     HLLGAAIS"
     gene            complement(12022..12432)
                     /locus_tag="PL152_01795"
     CDS             complement(12022..12432)
                     /locus_tag="PL152_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR48035.1"
                     /translation="MAENKAAQDAPPHVGVIDPDPVARNGLRALLHRNGVDVSTFDSA
                     ESYLLAANGRHLACLIVDLLLPGMSGLELLRRLRGAGNDVPVVLLADESDIPTAVAAM
                     REGATDFIEKPYVDVAVLKQVQKLLHRPPAATHG"
     gene            complement(12614..13234)
                     /gene="fixJ"
                     /locus_tag="PL152_01800"
     CDS             complement(12614..13234)
                     /gene="fixJ"
                     /locus_tag="PL152_01800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator FixJ"
                     /protein_id="HQR48036.1"
                     /translation="MKEVKPTVFVVDDDAAVRDSLRLLLKSVGLPVEVFESGQEFLDA
                     DRDDRPGCLVLDIRMPGMSGLELQVKLNERHSILPIIFITGHGDVPMAVEAMQAGAVD
                     FIQKPFRDQDLLDRINQALDKDAGSRRMLAERNMIRKRLESLTPREHEVLDLVVAGKA
                     NKVIAGDLNLSQRTVEIHRARVMEKMEAHSLAHLVRMVLEVERPDE"
     gene            complement(13241..14803)
                     /locus_tag="PL152_01805"
     CDS             complement(13241..14803)
                     /locus_tag="PL152_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR48037.1"
                     /translation="MNRRHTHEGMHRREPAARASRGNRDFRALLDAAVDAIVVIDHRG
                     IVEDFNLAAERMFGYQAAEVVGHNVSMLMPEPYRSQHDGHLARYVRTGQARIIGVGRE
                     VVAQRKDGSTFPSELAVGRVPDVEPARFVGFLRDLTEHKAAEEQLKRSEAELRLAQAL
                     ANLGNYVIHLQGDGQDYYSTQLYRILDVPVDQPAIARDEYFDKWVHPSDRARVVEAFH
                     RLDAGGTALDIEYKVALHDGRVRHVHHIAQAVRDEQGRVVKHVGAIHDITDRAHAEDE
                     ARQLQERLTHFSRLSTMGEMAAGLAHEINQPLSAIATYAQACQRILRQPEPDVADVAA
                     ALEQINAQALRAGEVIRRLRNFVKNREVKREPVDCGRLLEDLRTLAETDARLHNVNLH
                     IESDEDLPAVHADPIQLQQVVLNLVRNAIDAVADCPEGRREVRLRASRAPEGEIEITV
                     ADRGSGLAPQAAEHLFNPFFTTKVSGTGLGLAISRSIVRAHGGRLWHTPNHEFGAKFH
                     FTLPASPVTKGE"
     gene            14984..15421
                     /locus_tag="PL152_01810"
     CDS             14984..15421
                     /locus_tag="PL152_01810"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HQR48038.1"
                     /translation="MRLTRWEPFREMDEFFKGFTPMFGRMPATRPLEGMHEFMPLADI
                     VEHEKEYLIKIDLPEVPKEDVKVLFEEGVLTVKGERKVEKEVKGEKLHRTERYYGAFE
                     RSFVLPEDIDPKLIRAEAKEGVLVVHIPKVETPKAKPLAITIQ"
     gene            complement(15527..16279)
                     /locus_tag="PL152_01815"
     CDS             complement(15527..16279)
                     /locus_tag="PL152_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR48039.1"
                     /translation="MSLGAALLAGIAASGHCIAMCGGVAGALAMRNRDAGTGARIANA
                     LAYNLSRIASYTVAGAIAGLLGGALLQAVNVGALVVTFRTLSGLIMLAAAGKLLFGWR
                     LLDPVEAAGSSLWRRVAPWAGRQGHTAGVRGAVGLGLAWGWLPCGMTYSMLLLAATSA
                     SAPTGAAVMLAFGAGTLPSMATATLAFDRAARILSRRAAVKQVAGLLLLVFGLWTAGY
                     ALYHALAPGATHQHHHATTSDPPAGRMDCGHG"
     gene            complement(16300..16494)
                     /gene="ccoS"
                     /locus_tag="PL152_01820"
     CDS             complement(16300..16494)
                     /gene="ccoS"
                     /locus_tag="PL152_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009238367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome oxidase assembly protein
                     CcoS"
                     /protein_id="HQR48040.1"
                     /translation="MSIVYLLIPLGLVLVGIGLWAFFWAVGNGQFDDLDSPGWSVLSD
                     DEGPPTRPADVTSHDTKEQD"
     gene            complement(16496..>17131)
                     /locus_tag="PL152_01825"
     CDS             complement(16496..>17131)
                     /locus_tag="PL152_01825"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQR48041.1"
                     /translation="SWVCLGDERGLVAGFRLTDPLRAEAATCVGELQRLGLQSEIVSG
                     DEEASVARIAARCGVERHAARLTPDAKLARVKELQAAGSVVVAIGDGINDAPLLRGAD
                     AAVAMGRGSALAQTSAGMILVRDSLDELPHAVELARRTQRIVRQNLVWAIVYNLAALP
                     VAAFGLVPPWLAAIGMAVSSIAVVLNSMRLSQGGGDRRNTGWSLQAPVEDS"
     assembly_gap    17132..17231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17232..18961)
                     /locus_tag="PL152_01830"
     CDS             complement(<17232..18961)
                     /locus_tag="PL152_01830"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase
                     metal-binding domain-containing protein"
                     /protein_id="HQR48042.1"
                     /translation="MTPAGPACFHCGEPIPAGLVIHARVGTADEPVCCHGCKAVAEFI
                     AGAGLGDYYRYRDQAAARADEAARPDQWAAYDRPELVERLTRPEPGNARSITVLLEGM
                     RCSACGWLADQALRQQPGLRDASINPATARARLVWDPAAVRLGDLLRVLEHVGLRPHP
                     LAGDAAGQLALVERRTALKRLAVAGFGMMQVMMFAVPLYIGATSGMDPGIRELLRLVS
                     MLVSVPVALYSGWPFYEGAWQALRARGINMDVPVSLGIVLAFAASVWNALTGHGDVYF
                     DSVTMFVFFLSLGRYVEMTARHRSGSVADALARLAPVTARRVHGVDVEDVQAAELQPA
                     DEILVRTGEVFAADGVVVEGNGRADESMLTGESTAVAKGPGATVNAGTQNLGVPLRVR
                     VTAVADQTVLAGIVSLLERAQAEKPRLARAADRAASWFLGRILVGAAVVFAVWWFVDP
                     SRAFSATLAVLVVTCPCALSLATPAAIASATAALARRGVLVAHADALEALAKADHVLW
                     DKTGTLTRGLVRIEEVRPLTGLAETRCLELAAALERMSEHPIAKAFLTPGAAAAVATD
                     VEVHAGLGIEG"
     gene            complement(18958..19158)
                     /locus_tag="PL152_01835"
     CDS             complement(18958..19158)
                     /locus_tag="PL152_01835"
                     /inference="COORDINATES: protein motif:HMM:NF017559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FixH family protein"
                     /protein_id="HQR48043.1"
                     /translation="MNPNAPRPWYRQLWPWLLMLPPAGAVVGGALTYYLAVTRPDTLV
                     RQDCVQDGVTMKCGETDEHRAP"
     gene            complement(19171..20553)
                     /gene="ccoG"
                     /locus_tag="PL152_01840"
     CDS             complement(19171..20553)
                     /gene="ccoG"
                     /locus_tag="PL152_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR02745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008535 - respiratory chain complex IV
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase accessory protein CcoG"
                     /protein_id="HQR48044.1"
                     /translation="MNAPETEVDGGLYAARQKIYPREIDGRFQRLRTIAAWVLLGIFY
                     LIPWIPWGGRQAVLFDLPARKFYIFGLTLWPQDFYFLTWLLVIAALSLFFFTALAGRL
                     FCGYACPQTVWTETFLWMERFVEGSRSQQMKLAKAPWTADKVLRKAAKQALWIVFSLW
                     TGFTFVGYFTPVRELAGLIASFSTGPWETFWVLFYGGATYVNAGFMREQVCKYMCPYA
                     RFQSAMFDRDTLIITYDEKRGEPRGARKKGVDPKAKGLGDCVDCTVCVQVCPTGIDIR
                     KGLQYECIACAACIDACDEVMDRVGYPRGLIRYDTQHGLEGKPKHNVRPRTIVYASLL
                     VLLVAGFAYAITHRTVVGVDILRDRNALYRERPGGLVENVYNVKILNKDSEPHEFTIR
                     ASGLEDIEVDYAGPTVWVGPGQVQGVPTRIRVPRDEVEGGADIRVEIQATDRPDLHAG
                     GKARFLAPTD"
     gene            complement(20550..20885)
                     /locus_tag="PL152_01845"
     CDS             complement(20550..20885)
                     /locus_tag="PL152_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48045.1"
                     /translation="MLFGRHPGEYTGKLRWPRLGQDVAIVLWPAFLVASIATMVCFAF
                     IDPLLVGNDDYPPPAFASRMSGYAVGFFFFWLVATSSSLLTLYLVRTAHPEPPETDEN
                     ASGGSRSPE"
     gene            complement(20899..>21679)
                     /gene="ccoP"
                     /locus_tag="PL152_01850"
     CDS             complement(20899..>21679)
                     /gene="ccoP"
                     /locus_tag="PL152_01850"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit III"
                     /protein_id="HQR48046.1"
                     /translation="DTGHVWDKDLREYNNPLPRWWLWVFYITVAFGLVYLVLYPGLGN
                     FKGIKGWSQAAQYDQEKAAAEAKASTYYAQFAGMTVAQLAADPKAMATARNLFQVNCA
                     QCHGSDGGGAKGFPNLTDKNWQWGGDPDTVVQTITAGRIAAMPPWGPVLGLAGVDSVI
                     AYVQQISGQPTDATVAAAGKTVFETNCVACHGADGKGNPLVGAPNLTDDIWLYGGDSA
                     TLHETITNGRNGQMPAFGERLGAERVRLLAAYAIHLSESGS"
BASE COUNT         3366 a         7443 c         7499 g         3271 t
ORIGIN      
        1 ggccgaaggt ttcctcgcgc gcgaccatca tgtcgggcgt cacgtgcgtg acgatggtcg
       61 gctcgaagaa cgtgccgccg agcgcgtgac gcttgccacc gagcgcgacc ttgccgccct
      121 tggcgagcgc gtccgcgatg tgttcctcga ccttggcgac cgccttggcg tcgatcagcg
      181 ggccctgctc ggtcgcgccc gcgaggccgt cgccgacgcg cagcttgcgc acggcctcga
      241 ccagcttcgc gacgaacgcg tcgtacacgc cttcctgcac cagcagccgg ttcgcgcaca
      301 cgcaggtctg cccggtgttg cggtacttgc tcgcgatcgc gccctgcacg gcggcatcga
      361 ggtccgcgtc gtcgaacacg atgaacgggg cgttgccgcc gagctccatc gagaccttct
      421 tgagcgtgcc cgcgcactgc gccacgagct tcttgccgat ctcggtggag ccggtgaagg
      481 tgagcttgcg cacgatggga ttcgaggtca tctcgccgcc gatcgcggcc gcggagcccg
      541 tgacgacgct gaacacgccc ttcggcacgc cggcacggtg cgcgagctcg gccatcgcca
      601 gcgcggagta gggcgtctgc gtcgcgggct tgcacacgaa cgtgcagcca gcggcgagcg
      661 ccggaccagc cttgcgcgtg atcatcgcgg ccgggaaatt ccacggcgtg atggccgcga
      721 cgacgccgac tggctggcgc agcacgacga tgcgcttgtc gggctggtgg ccggggatca
      781 cgtcgccgta gacgcgcttg gcttcctcgg cgaaccactc gatgaaggag gccgcgtagg
      841 cgacctctcc cttcgcctcg gcgagcggct tgccctgctc ggcggtcatg atcaccgcga
      901 ggtcgtccgc gttcgcgagc atgaggtcgt tccagcgacg caggatgacc gagcgttcct
      961 tggcggtctt cgccgcccac gcggggaagg ctgccgccgc cgcttcgatg gcgcggcgag
     1021 tttccgccgc ctgcaggttc ggcaccgtgc cgatcacctc gcccgtcgcc gggttcgtga
     1081 cgtcgatctt gccgccgccg tccgcgtcga tccaggtgcc gtccacgtag cactgctggc
     1141 ggaggagcgt ggggtccttc agggtgaatg gcatggctgc gagtcctgct gggagccccg
     1201 gaacctgcgc cgggaccgaa tgaataggat atcaaatggg ggacatgctc ctttttcgcg
     1261 aagccgaaaa aggagaatgt ccccggttca gtactcgctg gtgcccttgc cgagcgtcac
     1321 gatggactcg acctccccgg ccacgaagcg cacgcgctgc atgagcttgt tcggcccgaa
     1381 gtcgtaagtc cacgagtcca ccggcacgtc ctcggtaccg gggaacgaat aggtccaccc
     1441 gccgagcgcg aactgcggcg gacgctggat ccaggtgcgg ctgcggatcg cgggttcgcc
     1501 gcacaccgcg agcacctttt ccatggtgtc gccgtccttg acgatgcggc tgccgcagcg
     1561 catcgagtcg gaacgcgcgg cgccgcacac gacgagcagc agggcgcctg cgccggcgac
     1621 gaggcttcgg gccgcggacc ggtcaatagc cataaccgtt ggtccggatc tcgtcgagtt
     1681 cgtcgccgac gaaccgcagg gtcctgatca gcttgttcgg cccgaagttg taggtccaga
     1741 tctcgaccgg cagcgcggtc cagcccggcc agaagtactg gtagccgtaa ggtccgtctt
     1801 cgtacagcgg cccgctgatg tagccggcga ggctgatgct ggtgggctcg ccgcacaggg
     1861 tgcgcacctt gtccttgccg tcgccctcgc tcacgaggcg cgtgccgcag cgcatggtgt
     1921 cgtcggcgct cgcggtcggg gccgcgaaca cgccaacggc cagcgcggcc agcagggcca
     1981 gcacggtccc ctggctggcg gtccgtcgtg cacgaaggct gcgtcgaggc ggcgagtgca
     2041 tgcttcatgt tacggcgatt cccgggcccg ccgggtatcc gcactaaccc gatgatccag
     2101 cggaggaatc gggccttcgc accggccgga cacccctgcg gcggtcgggc cggcgggtga
     2161 gtctggtacg ctttgcggct ccccctgtcg gcaccgtgac catggctcct tcccgcacct
     2221 tcgaccgccg cctggccgcc ctggtcaacc gccaggcgac ccgagcgctg cagggcggcc
     2281 tgaagggcgt cgagaaggag tccctgcgcg tcacgccccg gggactgatc gcgacgactc
     2341 cccaccccag ggccctgggg tcggccctca cgaacgagca catcacgacg gactactccg
     2401 aggcgctcat cgagctcgtc acgccgccgt ttccgcagac gtgggagctg gtgcagtacc
     2461 tgtgcgacct gcaccagttc gtgtaccggc acctcgccga cgagctgctc tgggcgacca
     2521 gcatgccgtg cgcgatcaag ggcgaggaaa gcatcccgat cgccgagtac ggctcgtcga
     2581 acgtcggcag gatgaagacc gtgtaccgcc gcggtctcgg ccatcgctac ggccgggtga
     2641 tgcaggcgat ctccggcgtg cacttcaatt actcgtttcc cgagcacttc tggccggtgc
     2701 tgggcgacct gctcgagagc cgcgacaccg gccaggcctt ccagtcggat gcgtacttcg
     2761 cgctgctgcg caactaccgg cgccacggct ggatcgtcct gtacctgttc ggcaactcgc
     2821 cggcgatctg cccctcgttc ctgcagggcc gcaaggtgga ctggctcgag ccgctcgagc
     2881 cgggcagcct gtacgcgccg cacgcgacgt cgctgcgcat gagcgacctc ggctaccgca
     2941 acaagagcca ggcaggaatg tcggtctcgg tgaacagcct cgagcactac gtgcgcgacc
     3001 tggcgcaggc gatcagcacg ccgcatgcgg agtacgagaa gctcggcgtg aaggtggacg
     3061 gcgaataccg gcagctgaat gccaacctgc tgcagatcga gaacgagtac tacagcttca
     3121 tacgcccgaa gcgcgtgacg atgtccggcg agcggccgac caaggccctg cggcgcggcg
     3181 gcgtgcagta cgtcgagatg cgctcactgg acgtcagcgc gttcgacccg gtgggcgtca
     3241 accagatgaa gctccggttc ctggaggcgt tcgcggccta ctgcgtcctc gcggcgagcc
     3301 cgccgatcga gccagcggag caggccgaac tcgacgggaa ccacgcgctc gtggcgcgcg
     3361 agggccgcaa gcccggcctc atgctccgac gcgacgggcg cgacctgtcg ctgcgcgact
     3421 gggcgtccga gatcgtcgac tcgatgcgcg gtgtctgcga gctgctggac gagggcgacc
     3481 cgcagcgcca ccactcggcc gcgctcgagg tgcaggcggc caaggtccgg gaggtggcgc
     3541 tcacgccgtc cgcgcgctcg ctcgaggaga tgcgcaccaa cgaggaatcg ttcttcaact
     3601 tcgcactgcg catgtcgaag ctgcacaagt cgtatttcct cgagctgcac ccgccgcacg
     3661 aggcgcggca gcaggcgttc gccgccgagg cggaggaatc gctgcaggcg caggcgaaga
     3721 tcgaggcgtc cgacacgatc ggcttcgacc agtacctcgc gcagtatttc gccggctagg
     3781 tgcctggcac cgattgagaa ataggggaca ttctcctatt tcccgtgcgg gaaataggag
     3841 aatgtcccct atttccgcag gatgccggtc tcgaagcggc cgcgggagtc catcgcgccg
     3901 cgcagcatgc ccggcgagtt gaacggcatc gcgatcgagc cgtcgcgacc gaccgcgatc
     3961 acgccgccgg aaccgccgag ccgcacgagc tgctgcatca cgacctcgtc cgccgcctcc
     4021 gacacgctca tcccgcggta gcgcatcagc gatgcgatct cgtgcgcgac gacggcgcgg
     4081 atgaagtatt cgccgtggcc ggtcgccgac accgcgcagc agtcgttcgc ggcgtaggtg
     4141 ccggcgccga tgatcggcga gtccccgacc cgtccccatt tctttgcggt cataccgccc
     4201 gtcgacgtcg cggcagccag gttgccgtgg gcatccagcg caacggcacc gacggtgcca
     4261 aggaccgctt cgtcctcgac gtccgcctcg ccgtgcaggt gccgctcgag ttcgaggcgg
     4321 cgccgctcgg tcgcgaagta ctcgttggga accggctcca gcccctgctg gatggcaaac
     4381 tcatcggcgc cggctcccgc gagcatgacg tgcggcgagc ggtccatcac caggcgggcg
     4441 agttcgaccg gattgcgcac gtggctcacg cccgacacgg atccggcgcg caggtcgcgg
     4501 ccgtccatga tcgacgcgtc gagttcgtgc agcccgtttg ctgcaagcac ggagccacgt
     4561 ccggcattga agagcggatc gtcctcgagc accttcaccg ccgccacgac ggcgtcgagg
     4621 ctgagcccgc cggactccat gacggcaaat ccggcgcgca gcgcagccgc gagtccgtcg
     4681 tggaacgcct gctggcgggc cgccgtcagg tcctttccgg gcatgacccc ggcaccgccg
     4741 tgcacggcaa ttgcgatcac gactgggtcc cctcgcgtgt cccgcgtccg tggctgtcga
     4801 tgcggccagc ctgcaccatc gggtgctcga tcggggccgg ccgctccatt ggaatcagct
     4861 tatcatccgg attccgccgc ctgcctgcca tcccgacacc gccatgcgcc agcccggaat
     4921 catcaggacc ctgctcgtcg ccaaccgcgg cgagatctcg atccgcgtga tgcgtgccgc
     4981 caacgaactc ggcatccgga cggtgtcgat ctactcgcag gaggaccgct tctcgctgca
     5041 ccggatgaag gccgacgaaa gctacctggt cggggaaggc aagggcccgg tcgaggcgta
     5101 tctcgacatc gcggacatca ttcgcgtcgc ccgtgaagcc ggcgccgacg ccatccaccc
     5161 cggctacggt ttcctgtccg agaacccgga gttcgcggaa gcctgcgcgg cggcagggat
     5221 catcttcatc gggccgaccg ccgagatcat gcggagcctc ggcaacaagg tgaccgcacg
     5281 cgcgctcgcc gtgtcggccg gcgtcccggt gatgcccgcc acgccgccgt tgccgcgtga
     5341 cgatgcggag tgcgcgcggc ttgcggcagg ggtcggctac ccggtgatgc tgaaggcgag
     5401 ctggggcggc ggcgggcgcg gcatgcgcgt cgtcgaaggc gaggcgcagc tcgccgagat
     5461 ggtggcgacc gcgcggcgcg aggccaaggc ggcattcggc aacgacgagg tctacctcga
     5521 gaagctcgtt cgcagggcgc gtcacgtcga ggtgcagatc ctcggtgatg cgcacggcaa
     5581 cctcgtgcac ctcttcgagc gcgactgcac ggtgcagagg cgcaaccaga aagtggtgga
     5641 gcgcgcgccg gccgtgtttc tcaccggcac gcagcgcgcg gagctgtgcg aactcgggct
     5701 gcgcatcggc cgtgcggtcc gctatctgaa cgcgggaacc gtggagttcc tgcaggacgc
     5761 cgacaccggc cagttctatt tcatcgaggt caacccgcgc atccaggtcg agcacaccgt
     5821 caccgaggtc gtgacgggca tcgacctcgt caaggcacag atccgcatcg ccgagggtgc
     5881 ggtgctagga cagcccggga gtggagttcc ctcgcagccc gacatccgca tgaacgggca
     5941 tgcgatgcag tgccgcatca ccaccgagga tcccgagaac gcgttcatcc cggactacgg
     6001 ccgcatcacg gcgtatcgca gcccggcggg tttcggcatc cgcctcgatg ccggcacggc
     6061 ctacaccggc gcattcatca cgcgttccta cgactcgctg ctggtgaagg tcaccgcctg
     6121 ggcgccgacg ccggacgaga ccatcgcgcg catgcatcgg gcgctgtggg aattccgcgt
     6181 gcgcggcgtg gtcacgaacc tgcgcttcct cgaccaggtg atcacgcacc cgcgcttcga
     6241 gaacgccgag tacacgacca ggttcatcga cgagacgccc gagctgttcc gcttcccgcg
     6301 caagcgcgac cgcgcgacgc gactgttgcg ctacatcggc gacgtgatcg tgaacggcag
     6361 tcccgaggtg atcgggcgcg cccggccgac gcgcacgctg gatccgcgac cgcccagggt
     6421 cgacctggcg acaccgcctc cgccggggac gaagcagcgg ctggacgagc tcggcccgcg
     6481 cgggttcgcg cagtggatgc tggcgcagga gccggtgctc ctcaccgaca ccacgttccg
     6541 cgacgcgcac cagtcactgc tcgcgacgcg cttccgcacg cgcgacatgg cggcggtggc
     6601 gccggcttat gcacggctgg ttccgaatct gctgtccgtc gaatgctggg gcggcgcgac
     6661 gttcgacgtc gccatgcggt tcctgaagga atgtccgtgg gaccggctct ccgcgttccg
     6721 cgaggccatg ccgaacctgc tgctgcagat gctgctgcgc tcggcgaacg ccgtcggcta
     6781 caccaattac cccgacaacg tcgtgcgcta tttcgtcgcg caggcggcgg cgaacggcgt
     6841 ggacctgttc cgcgtgttcg actccctcaa ctgggtcgag aacatgcgcg tcgcgatgga
     6901 cgccgtgctc gagaccgacc ggctgctcga ggcggcgatc tgctacaccg gtaacctcac
     6961 cgacccgagg cagacgaagt acgacctcga gtactacgtg cggctggcaa agcagctcga
     7021 gcgggccggc gcgcacgtcc tcggcatcaa ggacatggcg ggcctctgcc agccgcgcgc
     7081 cgcgtacacg ctcgtgaagg cactcaagga cgagatcggc atcccggtgc acttccacac
     7141 ccacgacacg agcggcatcg ccgcggcgag cgtactcatg gcggtcgagg cgggagtcga
     7201 cgcggtggat ggcgcgctcg acgcgatgag cggcctcacc tcgcagccca acctcggatc
     7261 gatcgttgag gcgctgcgcc acgggccgcg cgacaccggg ctcgacccgg agaacctgcg
     7321 gctgctgtcc aactactggg agcaggtgcg ccacggctat gccgccttcg agagcgacat
     7381 gcgcgcgggc gcgtccgagg tgtacgtgca cggcatgccc ggcggccagt acacgaacct
     7441 gcgcgagcag gcacgttcgc tcggcatcga cgacacgcgc tggcacgagg tcgcgcgcgc
     7501 ctacgcccag gtgaacgaga tgttcggtga catcgtcaag gtcacgccct cgtcgaaggt
     7561 cgtcggcgac atggcgatca tgatggtgac cagcggtctc agcgtcgggg aggtgctcga
     7621 tcccgacacc gaggtcgcat tcccggagtc cgtggtgtcg ctgttccgcg gcgacatcgg
     7681 gcagccgcag ggcggcttcc cgccggcgct gcagaagaag gtcctgaagg gcgccacgcc
     7741 gctcaccgtg cggccgggtg ccgtgatgcc gccggtcgac ctcgcccggc agcgcgagga
     7801 gctgcaggcg aagatgccgc gtcccatcag cgaccgcgag ttcgcgtcct ggctgatgta
     7861 cccgaaggtg ttcacggact acatgaccga acgcgtgcag tacggcgaca cgagcgtcct
     7921 gccgacgcgg accttcttct acgggctcgg ggccggcgag gagatcacgc tcgacctcga
     7981 gcgcggcaag cacctgatca tccgctacgt cacgacgagc gaggtgcacg acgacggaac
     8041 tcgcacggtg ttcttcgagc tgaacggcca gcctcggtcg gtaaaggtca ccgatcggag
     8101 caaggtcgcg aagcggccgc cgcgccgcaa ggcggaggcg gatcaccccg accaggtcgg
     8161 cgcgccgatg ccgggcacga tcgcgaccgt gcacgcggtc gccggccatc acgtgcagcg
     8221 tggcgacgtg ctggtcacca tcgaggcgat gaagatggag accagcgtgc gcgcggagcg
     8281 cgacggcacc gtggtcgagg tcgtcgcgcg ccccggggag caggtcgacg cgaaggacct
     8341 gctggtggtg ctcgcggaat ccggcaagtg acggagacgg acggctccgg gctcggggcc
     8401 cagctgcgcg agctctaccc ggcactggct ggattgcccg agcgtgacat cgaaatcctg
     8461 ctgtcgaggt gcagtgtgct cagcctgcga gccggggcgc agctgttcgg cgaggggtcg
     8521 gcgtgccggc agttcccgct ggtgatcgaa gggtcgatcc gggtcgcgaa gtgcggcgaa
     8581 ggacgcgagt tgcagctgta ccgagtgacg cccggcgaat gctgcgtgct caccagcagc
     8641 tgcctggtcg gcgggcgcga ctatccggcc accggtgtcg tggaaagcga cgtgcggctc
     8701 gtcgtgatgc cccaggccga gttcgagtcg ctgatggcgc gacacccgcc gttccgcaac
     8761 tacgtgttcg gattgttcgc cgagcggctg gccgacctga tgggcctggt cgaggcggtg
     8821 gcgtttcacc ggctcgaccg gcgcgtggcg agcgcgctgc tcggccacgg tcccgtcgtc
     8881 gagatgacgc accagcaact ggccgacgcc gtcggcagcg tgcgcgagat cgtgacccgc
     8941 gtgctcggga gcctcgcgga ccagggcgtg atccggctcg ggcgtggctc gatcgagatc
     9001 ctggacgccg tgaaattgcg acaggtggcg gagggcgcct agcgcggcgc ccccgcaggt
     9061 tccgcgagga cggcagggcg gctcggtgac tttggttaca gacccggatg cagcgtccca
     9121 atagcatcga ctccgggatc cggccgcagg ccggcatcga gaccattcgc aaggagcatt
     9181 cgaacatggc tgcaaacgtc ggcggcgtcg atcgcacgct gcgcatcgtc gtcgggatcg
     9241 tgctgatcgc gctctatttc gtcttgtcgg gcccggctcg ctacgtgggg ctgctggggc
     9301 tcgttgcgct gttcacgggg ctggtccgct tctgcccgct gtacacgctg ctggggatca
     9361 atacctgcgc gactaggaaa tagccgcgct cacccagcgg ttcagctgct gggcgcccat
     9421 cgcgcccgac tggcgcgcca cctcgcgtcc gtccttgaac acgatcaggg tcgggatgct
     9481 gcggatgccg tagcgcgcgg cgacgtcctg cgcctcgtcg gtattgagct tgccgaagcg
     9541 cacggcgggc tccatctgcc gcgcagtttc ctcgagtgcc ggggccatca tgcggcacgg
     9601 cccgcaccac ggtgcccaga aatccacgac cacgggcagg tcgcctcgcg tgatgtggga
     9661 gtcgaaattc ccgctgtcga gttccaccgg gtgaccggag aacagggact cgtggcagcg
     9721 ggggcagcgt ggatcgtcgc ccaggcggcc ctccgggaca ccgacgactc cattgcactt
     9781 ctggcagacg accttgcgcg acgacatggg tgctccgaac gatggaatcg cctgtcccgc
     9841 tgcatgggga cggagccggc ctgggacaag ccccccttgc cggacgaggt tcacgccacc
     9901 atgtaggggc tgatccggat aatggggaca ttctccattt tcggccgctg gcgaaaatgg
     9961 agaatgtccc cgtagattac gaccatgcgc tacaaggact actacaaggt catgggcgtg
    10021 gcgcgcgacg cgagccagga ggacatcaag cgcgcgtacc gcaagctcgc gcgcaagtac
    10081 cacccggacg tcagcaagga aaaggacgcc gaggagaagt tcaaggaact ccaggaagcc
    10141 cacgaggtcc tgaaggaccc cgagaagcgt gcggcctacg accagctcgg cgccgactgg
    10201 cgacccggcc aggatttccg cccgcccccg gactggggca agggcttcga attctcgcgc
    10261 ggcgcgcgtg gcggaggcgg cgagggcctc ggcgagttca gcgacttctt cgcggagctg
    10321 ttcggggagc gcagcccgtt cggtcgtccg ggcggcgggg ctcgtgctgg ccacggcttc
    10381 gctgcgcagg gccaggacca tctcgcccgc gtcgagatcg acctcgagga cgcctatcgg
    10441 ggcggcacgc gcgacatcga actgcgcgcg cccgagatca cgcccgacgg ccacgtgctc
    10501 accaggccgc gcaccctgcg cgtcacgatt ccggcgggcg tcaccgaggg gcagcagatc
    10561 cgcctcgcag ggcagggcag ccctgggctg ggcggcggcc ctccgggcaa cctgctgctc
    10621 gagatcggct tccgcccgca cgcgcgcttc aaggccgagg gacgcgacat caccctgacg
    10681 ctacccgttg cgccctggga agcggcactc ggcgaatcgg tgtcggtccc gacgctgggc
    10741 ggcccggtgg acatgaagct gccggccggc gcccgcgccg ggcagaagct gcgcttgcgc
    10801 ggccgcggac tgccgggccc ggcctgcggc gaccagtacg tgatcctgga aatcgtgctg
    10861 ccgcccgaca gtccgaaggc gcggcgcttc ttcgagcaga tgaagcgcga gttgccgttc
    10921 gacccaaggg ctgattgacg gtcagcgggg ggctcgggag cccgatccgg ccgatccgtg
    10981 gattgcggcg tctagctgat cgccgcgccg agcaggtggc cgatgatcca ggttaccgcg
    11041 cccgcgccgc cgccgatcgc gaccatgcgc aatccgccga gccacgcgtt gcgcccggtg
    11101 aacaggctca gcgcggcgcc cacgccgaac agcgcgatgc ccgcggtcgc ggccgcgacg
    11161 gtgaccgcgc gctcgagcgg caagccgagc aggaacggca gcaacggcag cgacgctccg
    11221 gcggcgaatg cgaggaacga aaaaatcgcg gcgccccatg gcgagccgag gtcgtccgga
    11281 ttcaggccga gttcctcgcg cgccagcgcg tcgagcgccg cgtcgtggtt cttcacgagt
    11341 tcgcgcgtga tgcggcgcgc ttcctcgagc tccatgccgc gcgtgttgta gatgagcgcg
    11401 agttcctcgg cttcctcctc cgggtactcg tgcaattcat cccgctcgag cccgatctgg
    11461 tactcgaaca tctcgcgctg cgagcgcatc gagacgtact cgcccgccgc catcgagagc
    11521 gcgccggcga gcagccctgc cacgccgcta gtaaccacgc caccgatacc tgcaccggca
    11581 ccggcgacgc ccatgatcag gctggcgttg gagacgaggc cgtcgttgac gccgaacacc
    11641 gcggcgcgca ggttgccgcc gccgtagccc ttgtggcgga cgccgacctg cgaaacggac
    11701 gtcggcatcg tgtggccggg cgtggcggtc cgggcgtcgt acgcggacac gccgcgcacc
    11761 ttcatcgcga cgagcaccgg cctgatgcgg cgtgggccca gcgtgcgcgc caggctggcg
    11821 gtgattctcg cgcgcaccga gggcgcgaac gcgggcggct cgccgccgga ttccgcccgc
    11881 cacttgtcgg cctggccttc ggcggacgaa gccagcgtgc ggaacaaggt cgcgatccgc
    11941 gcatcgggct cgcattcggc gagcgcgcgg tacagccacg cggactgctt ttcctcgcgc
    12001 cagctttcga ggccgctcac gtcagccgtg cgtggcggcg ggcggccggt ggagcagctt
    12061 ctgcacctgc ttcaggaccg cgacgtcgac gtagggcttc tcgatgaagt ccgtggcgcc
    12121 ttcacgcatc gcggccacgg ccgtgggaat gtcggactcg tcggcgagca gcacgaccgg
    12181 cacgtcgttg ccggcgccgc gcagccggcg cagcagctcg aggcccgaca tgccaggcag
    12241 cagcaggtcc acgatcaggc aggccaggtg tcgaccgttc gcggcgagca ggtaactctc
    12301 cgcgctgtcg aacgtggaga cgtccacgcc gttgcgatgc aggagcgcgc gcagcccgtt
    12361 ccgtgcgacc ggatcggggt cgatgactcc gacgtggggc ggagcgtcct gcgcggcctt
    12421 gttctctgcc atagataagg cgcgaccaca ggtcgggcca gttcaattcc agccgcaggg
    12481 aggatcggtt gcggcgaact gcgcagcgaa ctgtggtcgc atgggggcga gttttcccgc
    12541 ccgcaaaatc ccggagaatc ggggcttata cctaaaccgc ggcgacaatt ccgcggtgct
    12601 gcgccgcagt acgtcactcg tcggggcgct cgacctcgag caccatgcgg accaggtgcg
    12661 cgagcgagtg cgcctccatt ttctccatga cgcgcgcccg gtggatctcc accgtgcgct
    12721 ggctcaggtt caggtcgccg gcgatgacct tgttcgcctt gccggccacg acgaggtcga
    12781 gcacctcgtg ctcgcgcggc gtgagcgact ccagccgctt acggatcatg ttgcgctcgg
    12841 cgagcatgcg ccggctgccg gcgtccttgt cgagcgcctg gttgatgcgg tcgagcaggt
    12901 cctggtcgcg gaacggcttc tggatgaaat ccaccgcgcc ggcctgcatc gcctcgaccg
    12961 ccatcggcac gtcgccgtgg ccggtgatga agatgatcgg caggatcgag tgacgctcgt
    13021 tcagcttgac ctgcagctcg aggccgctca tcccgggcat gcggatgtcg agcaccaggc
    13081 agcccggccg gtcgtcgcga tccgcgtcga ggaactcctg gccggactcg aacacttcga
    13141 cgggcagtcc caccgacttc agcagcaggc gcagggaatc cctcaccgcc gcatcgtcat
    13201 cgaccacgaa gaccgtgggc ttcacttcct tcatcactgg tcattcccct ttcgtcaccg
    13261 gcgatgccgg cagcgtgaaa tgaaacttcg cgccgaattc gtggttcgga gtgtgccaca
    13321 ggcgaccgcc gtgagcccgc acgatggagc ggctgatcgc cagcccgagg cccgtgccgc
    13381 tcaccttggt cgtgaaaaac gggttgaaga ggtgctcggc ggcctgcggc gcgagtccgc
    13441 tgccgcgatc cgccaccgtg atctcgatct cgccctccgg cgcccggctg gcccgcagcc
    13501 gcacctcgcg gcgaccctcc gggcagtcgg cgacggcatc gatggcattg cgcacgaggt
    13561 tcaggaccac ctgctgcagc tggatcgggt cggcatggac cgcaggcagg tcctcgtccg
    13621 actcgatgtg gagatttacg ttgtgcaggc gcgcgtcggt ctcggcgagc gtccggaggt
    13681 cctcgagcag ccggccgcag tcgaccggct cccgcttgac ctcgcggttc ttcacgaaat
    13741 ttctcagccg gcggatcacc tcgccggccc tgagcgcctg cgcattgatc tgctcgagcg
    13801 ccgccgcgac gtccgcgacg tcgggctcgg gctggcgcag gatgcgctgg caggcctgcg
    13861 cgtaggtcgc gatcgcggag agcggctggt tgatctcgtg tgccaggccg gccgccatct
    13921 cgcccatcgt cgacagacgg ctgaagtgcg tgaggcgctc ctgcagctgg cgtgcctcgt
    13981 cctccgcgtg cgctcggtcg gtgatgtcgt ggatcgcgcc gacgtgcttc acgacgcgtc
    14041 cctgctcgtc gcgcaccgcc tgcgcgatgt ggtgcacgtg ccgcacccgc ccgtcgtgca
    14101 gcgccacctt gtactcgatg tcgagtgcgg tgccaccggc gtccaggcga tggaatgcct
    14161 cgaccactcg cgcccggtcc gacggatgga cccacttgtc gaagtactcg tcgcgcgcga
    14221 tggccggctg gtccaccggc acgtcgagga tccggtacag ctgcgtcgag tagtagtcct
    14281 gcccgtcgcc ctgcaggtgg atgacgtagt tgccgaggtt cgccagcgcc tgggcgagcc
    14341 tgagttccgc ctcgctgcgc ttgagctgct cctcggccgc cttgtgctcg gtcaggtcgc
    14401 gaaggaaccc gacgaagcgc gcgggctcga cgtccggcac gcgcccgacg gccagttcgc
    14461 tcgggaaggt ggaaccgtcc ttgcgctggg ccacgacctc gcgtcccacg ccgatgatgc
    14521 gcgcctgccc ggtgcgcacg tagcgcgcga ggtgaccgtc gtgctgactg cggtagggct
    14581 ccggcatcag catgctgacg ttgtgcccga cgacttcggc ggcctggtag ccgaacatgc
    14641 gctccgcggc gaggttgaag tcctcgacga tgccccggtg gtcgatcacg acgatggcgt
    14701 ccaccgccgc gtcgagcagc gcgcggaagt cgcggttgcc tcgcgacgcc cgggcggccg
    14761 gctcccggcg gtgcattcct tcgtgggtat ggcggcgatt catcggcggg ggcctctttg
    14821 agacgcaatg ctaagtgatg ccgcccggac ggactaggta ttacaggaga gccccttacg
    14881 cgccgttgcg aattacgcgc accgacccag ccgcggagaa tgaatccgcg gcccggcggg
    14941 tcccggccgc gccaagttac ccgggtgacc cgaggaggga gccatgagac tgacacgatg
    15001 ggaacccttc cgagagatgg atgagttctt caaggggttt acgcccatgt tcgggcggat
    15061 gcccgccacc cgtcccctcg aagggatgca tgagttcatg ccgctcgccg acatcgtcga
    15121 gcacgagaag gagtacctga tcaagatcga cttgccggag gtgccgaaag aggacgtcaa
    15181 ggttctcttc gaagaaggcg tgctcaccgt gaagggcgag cgcaaggtcg agaaggaagt
    15241 gaaaggcgag aagctgcatc ggaccgagcg gtattacggg gcgttcgagc gcagcttcgt
    15301 gctgcccgag gacatcgatc cgaagctgat ccgcgccgag gccaaggaag gggtgctggt
    15361 ggtccacatc ccgaaggtgg agaccccgaa ggccaagccg ctggcgatca cgatccagtg
    15421 accggatcgc ggccgcgagg agcggccagc ggcgtggcgg gccaccgctg agcggtgacc
    15481 gttgccaaag gaggaagagc ggccggcatt gccggccgtt cttcgctcag ccgtgcccgc
    15541 agtccatccg gccggcgggc gggtcactcg tcgtcgcgtg atgatgttga tgcgtggcgc
    15601 cgggcgcgag cgcgtggtag agcgcgtagc cggcggtcca caggccgaag accagcagca
    15661 ggagccctgc cacctgcttc acggccgcgc gccgcgacag gatccgcgcc gcccggtcga
    15721 atgccaacgt ggccgtggcc atggacggca gcgtgccggc gccgaacgcc agcatcaccg
    15781 cggcccccgt gggcgcgctc gcgctggtcg cggcgagcag cagcatcgag tacgtcatgc
    15841 cgcagggcag ccatccccag gcaaggccga ggccgacggc accgcgcacg ccagcggtgt
    15901 ggccttgtct cccggcccaa ggcgcgaccc gcctccagag cgaggagccg gccgcttcga
    15961 cggggtcgag caaccgccag ccgaacagca gcttgcccgc cgccgcgagc atgatcagcc
    16021 cggacagcgt gcggaacgtg acgaccagcg cgccgacgtt gacggcctgc agcagcgccc
    16081 ctccgagcag gcccgcgatc gcgcccgcga cggtgtaact cgcgatccgc gagaggttgt
    16141 aggcgagcgc gttggcgatg cgggcgcccg tgccggcgtc gcgattgcgc atggccagcg
    16201 cgccggcgac cccgccgcac atggcgatgc agtggccgct cgcggcgatg cccgcgagca
    16261 gcgcggcgcc gaggctcatg gaagagttca tttccacggt cagtcctgct ccttggtgtc
    16321 gtggctcgtc acatcggcgg gacgcgtcgg cggcccttcg tcgtcggaga gcacggacca
    16381 gcccgggctg tcgaggtcgt cgaactggcc gttgcccacg gcccagaaga atgcccacag
    16441 cccgatgccg accagcacga gcccgagcgg gatgagcagg tacacgatgc tcacgtcacg
    16501 aatcctccac cggcgcctgg agcgaccacc cggtgttgcg ccggtcgccg ccgccctgcg
    16561 agagccgcat cgaattcagc acgaccgcga tcgagctcac ggccatgccg atggccgcga
    16621 gccacggcgg gacgagcccg aatgccgcga ccggcagcgc cgcgaggttg tacacgatcg
    16681 cccacacgag gttctgcctg acgatgcgct gggtacgccg cgcgagttcg acggcatggg
    16741 gcagctcgtc cagcgagtcg cgcaccagga tcatgccggc gctggtctgc gcgagcgcgg
    16801 acccgcggcc catcgcgacg gccgcgtccg cgccgcgcag cagcggcgcg tcgttgatgc
    16861 cgtcgccgat cgcaacgacg acggagccgg cagcctgcag ctccttgaca cgcgcgagct
    16921 tggcatccgg tgtcaggcgg gcggcatggc gctcgacgcc gcagcgggct gcaatgcggg
    16981 cgacggaggc ctcctcgtca ccgctcacga tctcggactg caggcccagt cgctgcaatt
    17041 cgccgacaca cgtggccgcc tcggcgcgga gcgggtcggt gagacggaag cctgcgacga
    17101 gccccctctc gtcaccgagg cagacccagc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nccctcgatg ccgagccccg cgtggacctc gacgtcggtc gcgactgccg
    17281 cggccgctcc cggcgtgagg aatgccttgg cgatcgggtg ttccgacatg cgttcgaggg
    17341 cggcggccag ctccaggcac cgtgtctccg caaggcccgt gagcgggcgg acctcctcga
    17401 tgcgcaccag gccgcgcgtc agcgtgccgg tcttgtccca gaggacgtgg tcggccttcg
    17461 ccagggcctc gagcgcgtcg gcgtgcgcca ccagcacgcc gcgtcgcgcg agtgcagcgg
    17521 tggccgatgc aatcgccgcg ggcgtggcga gcgacagtgc gcagggacag gtgacgacca
    17581 gcacggcgag cgtcgcggag aacgcgcgcg atgggtccac gaaccaccag accgcgaaca
    17641 cgacggcggc gccgaccagg atgcggccga ggaaccagct cgcagcgcgg tcggccgcgc
    17701 gcgccaggcg cggcttctcc gcctgcgcgc gctcgagcag cgagacgatg ccggccagca
    17761 ccgtctggtc cgcgacggcc gtcacgcgta cgcgcagcgg caccccgagg ttctgcgtcc
    17821 cggcattgac cgtggcaccc gggcccttcg cgactgccgt cgattcgccc gtgagcatgg
    17881 actcgtcggc gcggccgttg ccttccacca ccacgccgtc ggccgcgaag acttcgccgg
    17941 tccgcaccag gatctcatcc gctggctgta gctccgcggc ctgcacgtcc tccacgtcga
    18001 cgccgtgcac gcgcctcgcc gtgacggggg ccagccgtgc cagcgcgtcg gcgacgctgc
    18061 ccgagcggtg gcgcgccgtc atctccacgt agcgcccgag cgacaggaag aacacgaaca
    18121 tggtcaccga gtcgaagtac acgtcgccgt ggccggtgag cgcgttccac acgctggcgg
    18181 cgaatgcgag cacgatgccg aggctcaccg gcacgtccat gttgatgccg cgcgcgcgca
    18241 gcgcctgcca ggcgccttcg tagaacggcc agccggaata cagcgcgacg ggcaccgaga
    18301 ccagcatgct cacgagccgg agcagttcgc ggatccccgg atccatgccg gacgtcgcgc
    18361 cgatgtacag cggcacggcg aacatcatca cctgcatcat gccgaagccc gcgaccgcga
    18421 gccgcttgag cgccgtgcgc cgctcgacga gcgcgagctg gccggccgcg tcaccggcga
    18481 gcggatgcgg cctgagcccc acgtgctcga gcactcgcag caggtcgccg aggcgaacgg
    18541 ctgcgggatc ccacaccagc cgcgcccgcg cggtcgccgg gttgatgctc gcgtcgcgca
    18601 ggcccggttg ctggcgcagt gcctggtcgg cgagccagcc gcacgccgag cagcgcatgc
    18661 cttcgagcag cacggtgatc gagcgggcat tgcccggctc cggtcgcgtc agccgttcga
    18721 cgagctccgg gcggtcgtag gctgcccact ggtcggggcg cgcggcctcg tcggcgcgtg
    18781 ccgccgcctg gtcacggtac cggtagtagt ccccgagccc ggcgcccgcg atgaattcgg
    18841 cgaccgcctt gcagccgtgg cagcagaccg gttcgtcagc cgtgccgacg cgggcgtgga
    18901 tgacgaggcc ggcaggtatc ggctcgccgc agtgaaaaca agccggaccc gcaggggtca
    18961 tggcgcccgg tgttcgtcgg tctcaccgca tttcatggtc acgccgtcct gcacgcagtc
    19021 ctggcggacc agcgtgtccg gccgcgtcac ggcgaggtag tacgtcaggg cgccgccgac
    19081 cacggcgcca gccggcggca gcatcagcag ccagggccac agctggcggt accacggtcg
    19141 cggggcgttc gggttcatgg gcgcagggta tcagtccgtg ggtgccagga aacgcgcctt
    19201 gcccccggcg tgcaggtccg ggcggtcggt cgcctggatt tcgacccgga tgtcggcgcc
    19261 gccttccacc tcgtcgcgcg gcacacggat gcgggtcggc accccctgca cctggccggg
    19321 acccacccag accgtcggtc ccgcgtagtc gacctcgatg tcctcgaggc cgctggcacg
    19381 gatcgtgaac tcgtgcggtt cgctgtcctt gttcaggatc ttgacgttgt acacgttctc
    19441 gacgaggccg ccgggccgct cgcgatagag cgcattgcgg tcgcgcagga tgtccacgcc
    19501 gaccaccgtg cggtgcgtga tcgcgtatgc gaatcccgcg accagcagca cgagcaggct
    19561 cgcatacacg atcgtgcgcg gccgcacgtt gtgcttcggc ttgccctcga gaccgtgctg
    19621 cgtgtcgtag cggatcagcc cgcgcgggta gccgacccgg tccatgacct cgtcgcaggc
    19681 gtcgatgcac gcggcgcagg cgatgcactc gtactgcagt cccttgcgga tgtcgatgcc
    19741 ggtcgggcag acctggacgc acaccgtgca gtccacgcag tcgccgagcc ccttcgcctt
    19801 cggatccacg cccttcttgc gcgcgccgcg cggctcgccg cgcttctcgt cgtaggtgat
    19861 gatgagcgtg tcgcggtcga acatcgcgct ctggaagcgc gcgtacgggc acatgtactt
    19921 gcagacctgc tcgcgcatga acccggcgtt gacgtaggtg gcgccgccgt agaacagcac
    19981 ccagaacgtc tcccagggtc ccgtcgagaa gctcgcgatg aggcccgcga gttcccggac
    20041 cggcgtgaag taaccgacga acgtgaatcc ggtccacagg gaaaagacga tccacagcgc
    20101 ctgcttggcg gccttgcgca ggaccttgtc ggcggtccag ggtgccttcg ccagcttcat
    20161 ctgctgcgag cggctgccct cgacgaaccg ctccatccac aggaacgtct cggtccacac
    20221 ggtctgcggg caggcgtagc cgcagaacag tctccccgcg agcgcggtga agaagaacag
    20281 cgacagcgcc gcgatcacca gcagccaggt gaggaaatag aagtcctgcg gccagagcgt
    20341 caggccgaag atgtagaact tgcgcgccgg caggtcgaag agcacggcct gccgcccgcc
    20401 ccacgggatc cacgggatga ggtagaagat gccgagcagc acccacgcgg cgatcgtgcg
    20461 cagccgctgg aagcggccgt cgatctcgcg cggatagatt ttctggcggg cggcgtacag
    20521 cccgccatcg acctcggtct ctggcgcgtt cattcggggc tccgagaccc gcctgaggcg
    20581 ttctcgtcgg tctccggggg ttccgggtga gcggtgcgga ccaggtagag ggtcagcagg
    20641 ctggacgagg tggccaccag ccagaagaag aagaagccga cggcatagcc gctcatccgc
    20701 gacgcgaacg ccggcggcgg gtagtcgtcg ttaccgacca gcaggggatc gatgaaggcg
    20761 aagcagacca tggtcgcgat gctggcgacc aggaaggcgg gccacagcac gatggccacg
    20821 tcctggccca ggcgtggcca ccgcagcttg cccgtgtatt ccccgggatg acgcccgaac
    20881 agcactccgg ggggcctcct acgagccgga ctccgacagg tggatcgcat aggccgcgag
    20941 cagtcgcacc cgctccgcgc ccaggcgctc gccgaacgcc ggcatctggc cattgcggcc
    21001 gttggtgatg gtctcgtgca gcgtcgcgga gtcgccgccg tacagccaga tgtcgtccgt
    21061 caggttcggc gcacccacga gcgggttgcc cttgccgtcg gccccgtggc acgcgacgca
    21121 gttggtctcg aagacggtct tgccagccgc tgcgaccgtc gcgtcggttg gttgcccgga
    21181 aatctgctgc acgtaggcga tgaccgaatc gacgcccgcc agcccgagca ccgggcccca
    21241 gggaggcatc gccgcgatgc gcccggccgt gatcgtctgc acgacggtgt ccgggtcgcc
    21301 gccccactgc cagttcttgt cggtgaggtt cgggaagccc ttggcgccac cgccgtccga
    21361 gccgtggcac tgcgcgcagt tcacctggaa gaggttgcgc gccgtggcca tggccttggg
    21421 atccgcggcg agttgcgcca cggtcatccc ggcgaactgc gcgtagtagg tcgaggcctt
    21481 cgcttccgcc gcggccttct cctggtcgta ctgggcggcc tgcgaccagc ccttgatgcc
    21541 cttgaaattg ccgaggcccg ggtagagcac caggtacacc aggccgaatg cgaccgtgat
    21601 gtagaagacc cagagccacc agcgcggcag cgggttgttg tactcgcgca ggtccttgtc
    21661 ccacacgtgc ccggtgtcg
//
LOCUS       DAOMFD010000015        20823 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_452_length_20823_cov_29.371244, whole genome shotgun sequence.
ACCESSION   DAOMFD010000015 DAOMFD010000000
VERSION     DAOMFD010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20823)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20823)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20823
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_452_length_20823_cov_29.371244"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..746
                     /locus_tag="PL152_01855"
     CDS             <1..746
                     /locus_tag="PL152_01855"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HQR48047.1"
                     /translation="EGEGDQAKWLGYDTRHLLADLTPKPDQVTIAINTMQMNITELMT
                     PDDVRGTQFENPAICNAWADMVVDATPTFYLEHLKRLREKGIQVYFMLGNTPQLETVE
                     RLIRRGQYMGPLNHCYVAIGGGASGPDPFAMMDYIRRSPQGSVLQIEGIMRNGPPMMA
                     MAIAMGLHVRVGIEDNIWRRKGERFTSVQQVEQVVRIARELGRDVATADDTRRIYKIG
                     TWYHSVDETLEALGMTPNRKPGRRGFLAA"
     gene            736..1875
                     /locus_tag="PL152_01860"
     CDS             736..1875
                     /locus_tag="PL152_01860"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQR48048.1"
                     /translation="MPREAKILKASAGESGRVEPAGPARPAPAAGPAEQGCPVGALVQ
                     LPDLMREAGHDPWSLLESFGVTQAMMAQPMTPIPLTLYGQILQAAADAIGRDDLGLLL
                     GQRATMENAGPLRLLVLNSRTTGEAVESYVRYGGLWYHGLKITMTLEQGYACIALSSD
                     RQFVGRDHVLTWMLAATVKHLEAIFPRSWRPSQVHISYRRPKQAEAYARFFRAPVLYD
                     QLRHAVFFPAAALDEVRPGSQANLDSFLRQYMGELEAREQPDFVSRVRRVIESLLASG
                     ECSIVRVAAMFSMHRVTLHRYLREQGTTFEDLLDDARRELAGRMLEQTNLPVGEIATA
                     LGYGNAGSFVRAFKRWNGVTPGAGRRRRPGIARAASPGRLAARRT"
     gene            1900..3222
                     /locus_tag="PL152_01865"
     CDS             1900..3222
                     /locus_tag="PL152_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR48049.1"
                     /translation="MAIPVQGQASAAQPHGEHSPVYAWAVFALTFGLMLSDYVSRQVI
                     TPIFPFLKVEWSLSDTELGALVSVVALIVGVASLPIALLADRWGRVKSITVMAGLWGL
                     ATIACGMARGYGQMMVARALVGLGEAGYGSAGGAILMHVFPPRRRAMIFAAFLAAALF
                     GSVGGVVAGGMISAHFGWRHAFVIVGVAGLLLVALYPVVVRDYKTVALTKSGPGESGR
                     KLRLGEVVRELFATRTAVFTYLASGSQMFTIGVINAWIPSYMSRYYGLAPDKAAMQAA
                     IVVLVAGVGMIVCGWSVDRFAVKVPINKLRAPAAYAVAACVLLVVAFALPPGQLQYAL
                     ILAGVFVSGSHTGATGAIIGDVTHPGLRATAFATIVLGNNLIGLSPGPVVIGALSDAF
                     GLRFALGVAPLACLVAAAFFALASRHYPNDTGRYEEPADSEAGPALVH"
     gene            3237..4058
                     /locus_tag="PL152_01870"
     CDS             3237..4058
                     /locus_tag="PL152_01870"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR48050.1"
                     /translation="MELAGKTAIVTGGGRGIGEGICHVLAREGANVVVMGRHANEVDA
                     VAAAIRASGGSAHAVVADVTDRNSLDAMTRATVDRFGGIDVLVNNAGIEGPPCLVEDL
                     PDDQWDRVLGVNLRGVFLCCKAVLPAMFARGRGRIVNISSIASQRMTFFGSVDYTVSK
                     YGVEGLTRHLAWELADRGITVNSICPGGVITPLMEENISPAFRDMVTKRMVPLGRMTT
                     MEEIGEAVSFLASERAQMITGQSIAVDGGLLTGFGEDLRAVIRKRMAEVKAAGGH"
     gene            4114..4884
                     /locus_tag="PL152_01875"
     CDS             4114..4884
                     /locus_tag="PL152_01875"
                     /inference="COORDINATES: protein motif:HMM:NF014664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HQR48051.1"
                     /translation="MTNKATHESGAGSQRAPGEETGPWGDALRQLQEWDPAWATTCTA
                     MATNPWRSGVLPRKLVELIGVMLNAQCTSLDPEGTRRHIRAALAAGASREEVLLVLKM
                     ASLGAIHSCSLGAPMLLEEARAAAVELRPGMAGEPTPFCDRMRAIGQWNAAWDPFYEL
                     DPVFTDQFMATGAAVYASGLLTPKEVELLSIAFDASYTHMYAPGTRRHIKGALQAGAT
                     VGEIMEVLKLCVVQGVQSCNLGVPILAEELARMGRAHD"
     gene            5001..6635
                     /gene="pgi"
                     /locus_tag="PL152_01880"
     CDS             5001..6635
                     /gene="pgi"
                     /locus_tag="PL152_01880"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF001211.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HQR48052.1"
                     /translation="MVAVRSVQSGPAWGALEEHAGRMRGVHLRSLFTDDAGRGDRLAR
                     EAAGVYLDYSKNRVTDETIRLLVRLADEAGLRQRIDAMFAGEKINATEGRAVLHVALR
                     APRGAHIVVDGENVVPQVHAVLDRMAAFAAQVRAGDWLGHTGRPIRNVVNIGIGGSDL
                     GPVMAYEALRHYSARELTFRFVSNVDATDFTEAVRDLDAAETLFIVASKTFTTQETMA
                     NARTARAWSVAGLGGDEASVARHFVAVSTNAGEVAAFGIDTANMFGFWDWVGGRYSMD
                     SAIGLSTMLAIGPNHFGEMLAGFHEMDEHFRTAPFERNLPALLGLLTVWYVNFLGART
                     VAILPYDQYLKRFPAYLQQLAMESNGKSVRLDGQPVGCDTGPVYWGEPGTNGQHSFYQ
                     LIHQGTHLVPCDFILFAQPSNPVGSHHDLLAANVFAQGEALAFGKTAAELRAEGCPEP
                     LVPHRSFEGNRPSNTLVLERLTPAALGRLVALYEHNVFTQGAIWGINSFDQWGVELGK
                     ALASRIVPELEAGVHPAGHDSSTGALIGRYRRLRGR"
     gene            6670..7497
                     /locus_tag="PL152_01885"
     CDS             6670..7497
                     /locus_tag="PL152_01885"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HQR48053.1"
                     /translation="MSMRLEAMPNFRDAGGHATADGRRIRTGVLYRSDQIGKLSDEDA
                     RRLSGIGIRFVYDLRTAGERATLSTERIPGATHVVLDVLADDRRATPAQVIALLGNAG
                     AAGDVLGDGKAAALMTESYRSFLQLESARAGFGQLYRELADAARVPALVHCTTGKDRT
                     GWACASLQLWLGVPYEAVLQDFLASNRYILPKYEPHLRAFAVAGGDPELLLPVLTVRE
                     EYLAASLHEMQRMYGSIDGYFSAGLGLGPVVQRTLRERFLDSSADHDAVKQGRRTLP"
     gene            7494..8768
                     /locus_tag="PL152_01890"
     CDS             7494..8768
                     /locus_tag="PL152_01890"
                     /inference="COORDINATES: protein motif:HMM:NF028178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HQR48054.1"
                     /translation="MNTATRRILIALVVVLAGALVWAAIAALRPLPERSLTMATGPDG
                     SAYRLYGERYRTLLAKQGVEVRLVETSGAQDNLARLLDAKSGVQAAFVQAGSTTEDQS
                     PGLVSLGTMFYEPLWIFSRSGSDQLALHDLVGRRISIGPRGGATHELALRLMRLNGLD
                     PAHLQLEEYPPERAAEELESGRIDVAIIDTAWDSPVVRGLLADTGVSVQGFPRADAYI
                     ALLPYLSKVVLPMGVASLSENRPPADVTLIAPKASLVVREDLHPALQYLLIDAAMKIH
                     GGPGVFNRAGEFPAAEMMDLPLSEEANHVYRSGPSFLRRHLPFWLAELAQRLLILLIP
                     LAGLVYPLSRLVPEAWRWEMGRRLRGMYRELLRIEQALRRASPGPERVEMLRKLDDLE
                     VRAAAIRLPDSFAAPAYELKQNIRFVQEKYAR"
     gene            complement(8832..10097)
                     /locus_tag="PL152_01895"
     CDS             complement(8832..10097)
                     /locus_tag="PL152_01895"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HQR48055.1"
                     /translation="MPADLASVTTAATAEESNPALVGMTREGVDAIWKSVESLYATGM
                     HPGISVVLRRNGRIVLKRAIGHARGNGPGEAGEPKVAMTPETPVCIFSASKAMAAMTV
                     HLLSERKHLSLLDPVSHYIPEFGQNGKRDITIYQLLCHKAGIPTIPTEGLDVPQLLLD
                     SKEVLRLIYRTAPDRPGQHHAYHALTSGFVIADLVERVSGENFRTFFRRNVSEPLGLA
                     TLDFGARGRALSRVARNYVSGFRLTGVTDMYLKRAIGTSLRHAVEVSNTAAYYRAVIP
                     AGNGVGTADECSKFFQCLLDGGKLGRTRVFQPLTVRRAIAEVGKPEIDKSLMVPLRYS
                     AGMMLGSSRFGLYGPDTSKAFGHLGFANIFAWADPERGISASLLTTGKLLLGVHAPQL
                     IAFLCNISRHCPKLSEAEQEQQLAAAGMA"
     gene            complement(10160..10774)
                     /locus_tag="PL152_01900"
     CDS             complement(10160..10774)
                     /locus_tag="PL152_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017405992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HQR48056.1"
                     /translation="MIEFFFDCSSPWTWLAFHNIQPMARELDVAIRWRPVLVGGIFNA
                     VNPSVYEFRERGVPAKQAYLKKDLQDWARLAGLKIVFPPTVFPVNSVKAMRGCILLEA
                     EGKLVPFAQAVFEAYWTHDRDISQDAVLAGICARVGADPQGFFAGIATPEIKAALKAN
                     TEEVISRGGFGSPTIFVGDDMYFGNDRLGLVREAVLRRKAAGGQ"
     gene            10814..11509
                     /locus_tag="PL152_01905"
     CDS             10814..11509
                     /locus_tag="PL152_01905"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQR48057.1"
                     /translation="MIDLYTAPTPNGHKVSIALEELALPYTVHVINLYKLEQRRPEFL
                     KISPNGRIPAIVDRDLGDFAVFESGAILIYLAEKTGRLMPADVKGRSLVMQWLMFQMG
                     GIGPMMGQANVFFRYLPEKIQPAIDRYHAEVRRLFEVLDARLADHEYLAGDYSIADIA
                     NWAWVRTHRWSGLELDGLEHLTRWKRQLAARPACQRGIEIPPNPLKGADATAARKFAE
                     AARQMVVTGHEPT"
     gene            complement(11571..12032)
                     /locus_tag="PL152_01910"
     CDS             complement(11571..12032)
                     /locus_tag="PL152_01910"
                     /inference="COORDINATES: protein motif:HMM:NF019301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HQR48058.1"
                     /translation="MIGGPSIVRLNDLASRVAAVFQDLLLLAIRWYVAWQFLKSGWLK
                     LTTWDTTLYLFQEEYHVPVLPPGIAAIAGTTGELLFPSLLVIGLLSRYAAIGLSFVNV
                     MAVVSYAHVLLSPGFEGALGQHYLWGSLLLVVAVFGPGRWSLDEYVAPRLR"
     gene            complement(12029..12823)
                     /locus_tag="PL152_01915"
     CDS             complement(12029..12823)
                     /locus_tag="PL152_01915"
                     /inference="COORDINATES: protein motif:HMM:NF021364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HQR48059.1"
                     /translation="MTRFAAALRTGTAADDAARDMASQIVDDGLEPGRRLRIYRNNVQ
                     AMFYGALSRTYPVLKATLGDATFESLAREYRGAHPSRSGDLHWVGQALPDWLHERLAD
                     DEDDSWLADLARLEWACEEALVARAMQPLQRDALASVPPEQLADIGLEIQPGVRVVSS
                     PHPIWSVWRAHQPGCDVEGMDSAAGPQHVVVACIAGQLALRSVPREPADFIRHLRDGG
                     TLGAALDASTLPVAALPGVLDWMFEDGLVVGLRPAATPACTGGAEA"
     gene            complement(12846..13718)
                     /locus_tag="PL152_01920"
     CDS             complement(12846..13718)
                     /locus_tag="PL152_01920"
                     /inference="COORDINATES: protein motif:HMM:NF003818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HQR48060.1"
                     /translation="MRDAVSSRVPGASIPASAGIGLRSPHYAHVAAGTPAVAWVEVHT
                     ENYFHDGGPHLHELERVRESHALSLHGVGLGLGSVESLDRAHLERVKRAVRRFEPALV
                     SEHACWGHSGGEHFNDLLPLPYTEEAVEHLSARVREVQDFLGRQILIENLSSYFAYSA
                     SRLTEWEFLAAVAERSGCGLLLDVNNVYVNSVNLGLDAARFIDSVPASAVQEIHLAGH
                     RRNPVGGRDILIDDHGSAVCEDVWALYARAIDRLGRVPTLIEWDTDLPEFEVLLAEAR
                     KANAILERCGELAA"
     gene            complement(13726..13986)
                     /locus_tag="PL152_01925"
     CDS             complement(13726..13986)
                     /locus_tag="PL152_01925"
                     /inference="COORDINATES: protein motif:HMM:NF021559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HQR48061.1"
                     /translation="MDVKKIAIAAAVGSFLAVGASGIAAAGDTPSKDKCYGVAKAGKN
                     DCAGNGHSCAGQAKKDNDPNEWKHMSKEDCEKAGGKTTPPAK"
     gene            complement(14234..16756)
                     /locus_tag="PL152_01930"
     CDS             complement(14234..16756)
                     /locus_tag="PL152_01930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48062.1"
                     /translation="MRVTNPMSRVLRGGIAAFLAAGSIAPALAGGPLYVTPVNGVMTP
                     LHWVGTVPVHLDSGNLNVGTDCVRDPVTYECVLDGAGNPVIPVLDQKAGDDLVAATVY
                     QWSHVPTSSFRAQVVDRSPVDITGANVSDYIGTYNGGGIQTIYDADNSVIDALTGGDG
                     YGVLGIASPEYASDSDPTQIIEGWQVIGGSFLNATSTEQISGVVTHEFGHAINLAHTQ
                     VNGFLQMNDPTSWSFYAGGNEQAGPDQCGPAAAGYPAANQIETMYPMIDPFPQSPTYN
                     SPEMAKVLDDADDMAALSSIYPATGYAATTGTIHGRIVAKDGKSQLTGINVIARRADK
                     PLTGAISRISGDSTQGLLGADGSFTMTGLVPGVNYLLYIDQLRAGAYSTPKALLLGPE
                     EYWNAGESGDASKDDACASTPISVAAGETREITIAVNGIARAPGFTVIPYVMPQVVSD
                     NGQKVAGIYGPSNESPYWVWDKTSFAKTGDVTFIGGSGVTIAMSGNGRVVGGTVAGPT
                     VTTSWGDVVQQRAALWTKEGGWKTITKDNWPGCDIFHTSIFDLSTDGSTAVGLAFKDC
                     THVYGFKWNARTGVKLLSKVGTDSARANAVSGNGGVVVGWQDMNDTFPYRIGSIWQGS
                     EQTILREPRSVSDYNPTGYVGEVLAINSAGNVAVGYNAGPDLNDSFIWTPYAGTRNLG
                     GDRKPFCFKTWDDTGAETTVCNRRQTIAYAISDDARVITGETSYNDFWNASAIDGAIF
                     TPKLGWMQLSAFLQSQGVLEATNWVFLGTKVSADGKTLIGTGAPLGADYYQGYRIDLD
                     QVFVCAGRGRYAKTLKVDFPIGMDIALAAGAKIGLCPGDAPL"
     gene            complement(16743..17396)
                     /locus_tag="PL152_01935"
     CDS             complement(16743..17396)
                     /locus_tag="PL152_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48063.1"
                     /translation="MISSHFSRRMRVFARASLAALALGAASPSSHATSVIPMTVVDLL
                     GHSDTIVVGQVQSVTDGFDAHGLPYTEVTLRVSDTIRGQSAGTYTFRQFGLDKPRTLE
                     DGRIYLGRPTGWPTWRKDESAIVFLYGKSRTTGLQTSVGLGQGKLSVANGVAVNSNDN
                     ASLFSGVRAKAGLLDANEQQMFSTKKGPVDADTFRKFLHRAVGGNWVKNGSLSNARH"
     gene            complement(17665..18957)
                     /locus_tag="PL152_01940"
     CDS             complement(17665..18957)
                     /locus_tag="PL152_01940"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR48064.1"
                     /translation="MSRRTLFRAHRGLLVLTGLVAFSAAHAQDVLIRGAQVHTETERG
                     TMERADVLVRDGRIVAVGSGLAAGPGASVVEANGRPLTPGLFGGITALGIEEVSAEPS
                     TVDSSVSYGDGKPGPEFQPRPEFDVSPAFNADSVAIGVNRVEGITYTMVAPTALPDGT
                     MFAGQGSIARLDGRADAFLPASRTLFIDLGGNATEGSGKSRAAQFMLLDQATRESLPG
                     RPLAAGDMRLLTPTGREVLARYLTGGRMAFDVDRAADIRQVLAYAARNGVHPVIVGGA
                     QAWQVAALLAKAQVPVVLNPLVDLPGDFDQLGATMENAARLHRAGVKVAFTTGETQNA
                     RKVRQAAGVAVAHGLPWDAALTGLTSAPAEIFGVGAQLGRIAPGYAADLVLWSGDPLE
                     VTTAAEMVWIGGQAQSMRSRQTELRDRYRPKATAPTAN"
     gene            complement(18954..20378)
                     /locus_tag="PL152_01945"
     CDS             complement(18954..20378)
                     /locus_tag="PL152_01945"
                     /inference="COORDINATES: protein motif:HMM:NF014081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR48065.1"
                     /translation="MGGVHVVLRSFLLAGIAAIAAPALADVDPYSSTYVAHPGAPTLL
                     RNATVLDGTGKRLDGADVLIRDGKIVAVGPGLDPGDAVVVDATGRWITPGIIDVHSHL
                     GVYASPGVRATQDGNEATDPVTAGVWAEHSVWPQDPGFSTALAGGVTTLQVLPGSANL
                     IGGRGVTLRNVHATTYQAMKFPGAPQGLKMACGENPKRVYGNKGRAPSTRMGNVEGYR
                     QAFADAQDYQRQWDKYARELAEYEGKQAKGKVKDDGKDEGPPKQPKRDLRLETLAAAM
                     RGELQVHIHCYRADEMATMIDLSHEFNFRIAAFHHGVEAYKIADRLAQEGICGALWAD
                     WWGFKMEAYDGIQENLAFVDAPANGCAIVHSDSEEGIQRLNQEAAKAMSRGQRAGLDI
                     PPERAIRWLTSNPARALGIADRVGTLEPGRAADVVVWNSNPFSVYALADLVYIDGAVV
                     LDRKSPPPAPASDFLLGQPTGGAR"
     gene            20514..>20823
                     /locus_tag="PL152_01950"
     CDS             20514..>20823
                     /locus_tag="PL152_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48066.1"
                     /translation="MRLHVDVRHAPVAIAASLLFVGGCGEKAPEPAPVAQAGPPPTPT
                     VQQLKSATVSGVFGEPVALADGKYDGPPPTPGAASHPTALLWDSAVVFGDVDGAPGSE
                     A"
BASE COUNT         3250 a         7003 c         7210 g         3360 t
ORIGIN      
        1 cggagggcga gggcgaccag gccaagtggc tcggctacga cacgcggcac ctgctcgcgg
       61 acctcacgcc caagcccgac caggtgacca tcgcgatcaa cacgatgcag atgaacatca
      121 cggagctgat gacccccgat gacgtcaggg gcacgcaatt cgagaacccg gccatctgca
      181 atgcgtgggc cgacatggtc gtcgatgcga cgcccacgtt ctacctcgag cacctgaagc
      241 ggctgcgcga gaaaggcatc caggtgtact tcatgctcgg caacacgccg cagctcgaaa
      301 cggtcgagcg gctgatccgc cgcggccagt acatgggccc gctcaatcac tgctacgtcg
      361 cgatcggcgg cggcgcctcg ggccccgacc cgttcgcgat gatggactac atccgccgct
      421 cgccgcaggg cagcgtgctg cagatcgagg gcatcatgcg caatggcccg ccgatgatgg
      481 cgatggcgat cgcgatgggc ctgcacgtgc gcgtcggcat cgaggacaac atctggcgcc
      541 gcaagggcga gcggttcacg tccgtgcagc aggtcgagca ggtcgtgcgc atcgcgcgcg
      601 agctcggccg cgacgtcgcg actgcggacg atacccgtcg catctacaag atcggcacct
      661 ggtaccacag cgtggacgag acgctcgagg ccctcggcat gaccccgaac cgcaagcccg
      721 gccggagagg cttccttgcc gcgtgaggcg aaaatcctga aggcgtccgc tggcgagtct
      781 ggtcgcgtcg agcctgcggg cccggcgcga ccggcgcctg cggctggccc cgcggagcag
      841 ggttgcccgg tcggcgcact ggtccagctg ccggacctga tgcgcgaggc cggccacgat
      901 ccgtggagcc tgctcgagtc cttcggcgtg acgcaggcca tgatggcgca gccgatgacg
      961 ccgatcccgc tcacgctgta cggccagatc ctgcaggcgg cggcagacgc aatcggccgc
     1021 gacgacctcg ggctgctgct cgggcagcgg gccaccatgg aaaacgcagg cccgctgcgc
     1081 ctgctggtgc tcaattcgcg tacgacgggt gaagccgtcg agagctacgt gcggtacggc
     1141 ggcctgtggt accacggcct caagatcacc atgacgctgg aacaaggata tgcgtgcatc
     1201 gcgctgtcct cggaccggca gttcgtcggc cgcgaccacg tgctcacctg gatgctcgct
     1261 gcgaccgtca agcacctcga ggcgatcttc ccgcgcagct ggcggccgtc gcaggtgcac
     1321 atttcctacc gtcggccgaa gcaggcggaa gcctacgcac ggttcttccg ggcgccggtg
     1381 ctctacgacc agctccgcca cgcggtgttc tttcccgcgg cggcactcga cgaggtgcgc
     1441 ccggggtcgc aggcgaacct cgactcgttc ctgcgccagt acatgggtga actcgaggcg
     1501 cgcgagcagc cggacttcgt gtcgcgcgtg cggcgcgtca tagagagcct gctggcgtcc
     1561 ggcgagtgct ccatcgtgcg cgtcgcggcg atgttctcca tgcaccgcgt cacgctgcac
     1621 cgttacctgc gcgagcaggg cacgaccttc gaggacctgc tggacgatgc gcgccgcgag
     1681 ctggcgggtc gcatgctcga gcagaccaac ctgccggtcg gcgagatcgc caccgcgctc
     1741 ggttacggca atgcaggcag cttcgtgcgc gcattcaagc gctggaacgg cgtgacaccc
     1801 ggggccggcc ggcgccggcg tcccggcatc gcgcgagcgg cgtctccggg acgcctggcc
     1861 gcgcgacgga cataagacaa gaagccaggg ggcgaagaga tggcaattcc ggtccagggg
     1921 caggcgagcg cagcccagcc gcatggcgaa cactcgccgg tctatgcgtg ggcggtgttt
     1981 gcgctgacgt tcggcctgat gctctccgac tacgtgtcgc gccaggtcat cacgccgatc
     2041 tttcccttcc tcaaggtcga gtggtcgctc agcgacaccg agctcggagc cctggtcagc
     2101 gtggtggcgc tgatcgtcgg tgtcgcctcg ctgccgatcg cgctgctcgc ggaccgctgg
     2161 ggccgcgtca agagcatcac ggtgatggcg ggcctgtggg gactcgcgac catcgcctgc
     2221 gggatggcgc gaggctacgg ccagatgatg gtcgcgcgcg cgctggtcgg tctcggcgaa
     2281 gccggttacg gcagcgccgg tggcgcgatc ctgatgcacg tcttcccgcc gcggcgccgg
     2341 gcgatgatct tcgccgcgtt cctggcagcg gcgctgttcg gttccgtcgg cggcgttgtc
     2401 gcgggcggca tgatctcggc gcacttcggc tggcggcacg ccttcgtgat cgtgggcgtc
     2461 gcgggcctgc tgctggttgc gctctatccc gtcgtcgtgc gggactacaa gaccgtcgcg
     2521 ctgacgaagt ccgggcccgg cgagtccggg cgcaagctcc gcctcggcga ggtggtgcgt
     2581 gaactgttcg ccacgcgcac ggcggtgttc acctatctcg ccagcgggtc ccagatgttc
     2641 acgattggcg tgatcaatgc gtggatcccg agctacatga gccggtacta cgggctcgcg
     2701 ccggacaagg cggcgatgca ggcggcgatc gtggtgctcg tcgcgggcgt cggcatgatc
     2761 gtgtgcggct ggagcgtcga ccgtttcgca gtcaaggtgc cgatcaacaa gctgcgtgcg
     2821 ccggcggcgt acgcagtggc ggcctgcgtg ctgctggtcg tcgccttcgc gctgccgccg
     2881 ggccagttgc agtacgcgtt gatcctggcg ggcgtgttcg tcagcggctc gcacaccggg
     2941 gcgaccggcg cgatcatcgg cgacgtcacg cacccgggcc tgcgcgcgac ggcgttcgcg
     3001 accatcgtgc tcggcaacaa cctgatcggg ctgtcgcccg ggcccgtcgt catcggggcc
     3061 ctgtccgacg cattcggcct gcgcttcgcg ctgggcgtgg cccctctcgc gtgcctggtc
     3121 gcggcggcgt tctttgcgct cgcctcgcga cactacccga acgacaccgg ccgctacgag
     3181 gagcccgcgg actccgaggc gggaccggca ctggtgcatt gaccggagaa aagctcatgg
     3241 aactggctgg caagactgcg atcgtgacgg gtggaggccg gggcatcggg gagggcatct
     3301 gccacgtgct cgcccgcgaa ggtgccaacg tggtcgtcat gggacgccac gcgaacgagg
     3361 tggacgcggt tgcggcggcg attcgcgcct cgggcggcag cgcgcatgcc gtggtggccg
     3421 acgtcaccga ccggaactcg ctcgatgcga tgactcgcgc gacggtcgat cggttcggcg
     3481 ggatcgacgt gctggtgaac aatgccggca tcgagggacc gccgtgcctc gtcgaggacc
     3541 tgcctgacga ccagtgggac cgggtgctgg gagtcaatct ccgcggcgtg ttcctgtgct
     3601 gcaaggcggt gctgccggcg atgttcgcgc gtggtcgcgg gcggatcgtc aacatcagct
     3661 cgatcgcttc gcagcgcatg accttcttcg gcagcgtgga ctacacggtt tcgaagtacg
     3721 gagtcgaggg cctgacccgg cacctggcct gggagctggc ggaccgcggc atcacggtca
     3781 attcgatctg ccccggcggc gtgatcacgc cgctcatgga ggagaacatc tccccggcat
     3841 tccgcgacat ggtcacgaaa cgcatggtcc cgctggggcg gatgacgacg atggaggaga
     3901 tcggcgaggc ggtgagcttc ctggcgagcg agcgagccca gatgattacc gggcagtcga
     3961 tcgcggtgga cggggggctg ctcaccggct tcggcgaaga cctgcgtgcc gtgatacgca
     4021 agcgcatggc ggaggtcaag gcggcaggcg gacactgagg gcgccgcggc cgagagccac
     4081 gcggcaacgt gaatgacagc gggtgacacg aacatgacga acaaggcgac gcacgaatcg
     4141 ggagccggct cgcaacgcgc gccgggtgag gagaccggtc cctggggcga cgcgctgcgg
     4201 cagctgcagg agtgggatcc tgcgtgggca acgacctgca cggcgatggc gacgaatcca
     4261 tggcgcagcg gcgtgctgcc ccgcaagctc gtggaactca tcggcgtgat gctcaatgcc
     4321 cagtgcacca gcctcgaccc ggagggtacc cgccggcaca tccgcgctgc gctcgccgca
     4381 ggcgccagcc gggaggaggt gctcctggtg ctgaaaatgg cgtccctggg cgcgatccat
     4441 tcgtgcagtc tcggtgcgcc gatgctgctg gaggaggccc gcgcggctgc ggtcgagctc
     4501 cgtccgggca tggccggcga gccgacgccg ttctgcgaca ggatgcgggc gatcgggcag
     4561 tggaacgccg cctgggaccc gttctacgag ctcgacccgg tgttcaccga ccagttcatg
     4621 gcgacgggcg cggcggtcta cgcgagcggc ctgctgacgc ccaaggaagt cgagctgctc
     4681 agcattgctt tcgatgcgtc ctacacccac atgtatgcgc cgggcacgcg ccgacacatc
     4741 aagggcgcgc tgcaggccgg ggcgacggtc ggggaaatca tggaagtgct gaaactgtgc
     4801 gtggtgcagg gcgtgcagtc gtgcaacctc ggggtgccga tcctcgccga ggaacttgcg
     4861 cgcatgggcc gcgcgcacga ctgagcgggc tcgccgcgtg cttgcatggc aggcaggatc
     4921 ccacgctaga ctctcgacag gaaccggcac gccgccggcc gtacacggcg gcgggcgcgg
     4981 agtccggtgc aaggagtctc atggtcgcag tacgatcggt ccagtccggc cccgcatggg
     5041 gagcgctcga ggagcatgcc gggcgcatgc gcggcgttca cctgcgctcg ctgttcaccg
     5101 acgatgccgg acgcggtgac cgtctcgcgc gcgaggccgc cggcgtctac ctcgactact
     5161 cgaagaaccg cgtcacggac gagacgattc gcctgctcgt gcgcctcgcc gacgaggcgg
     5221 ggctgcggca gcgcatcgac gcgatgttcg cgggcgagaa gatcaatgcc accgaaggcc
     5281 gggccgtgct gcacgtcgcg ctgcgggcgc cgcgcggggc gcacatcgtc gtcgacgggg
     5341 agaacgtggt gccgcaggtg cacgccgtgc tcgatcgcat ggccgcgttt gccgcgcagg
     5401 tccgtgccgg ggactggctg gggcacacgg gccggcccat acgcaacgtg gtcaacatcg
     5461 gcatcggcgg gtccgacctc ggcccggtga tggcctacga ggcgctgcga cactacagcg
     5521 cgcgcgaact gacgttccgc ttcgtgtcga acgtggacgc cacggatttc acggaggccg
     5581 tgcgcgacct ggacgccgcc gagaccttgt tcattgttgc gtccaagaca ttcacgaccc
     5641 aggagaccat ggcgaatgca cgcaccgcgc gggcgtggtc ggtcgccggt ctcggcggcg
     5701 acgaggcctc ggtcgcacgg catttcgtcg ccgtgtccac caacgccggc gaggtcgccg
     5761 cgttcggcat cgacacggcc aacatgttcg gcttctggga ctgggtcggc ggccgctact
     5821 ccatggattc cgcgatcggt ctgtcgacga tgctggccat cgggccaaac cacttcggcg
     5881 agatgctcgc cggcttccac gagatggacg agcacttccg gacggcgccg ttcgaacgca
     5941 atctcccggc actgctcggc ctgctgaccg tctggtacgt gaacttcctc ggggcacgca
     6001 cggtcgcgat cctgccctac gaccagtacc tgaagcggtt ccccgcctac ctgcagcaac
     6061 ttgcgatgga gagcaacggc aagagcgtcc ggctcgacgg gcagccggtc ggctgcgaca
     6121 cgggcccggt gtattggggc gagccgggga cgaatggcca gcattccttc taccagctca
     6181 tccaccaggg cacgcacctc gtgccgtgcg atttcatcct gtttgcacag ccgtcgaacc
     6241 cggtcgggtc gcatcacgac ctgctcgcgg ccaacgtgtt cgcgcagggc gaggcgctgg
     6301 cattcggcaa gaccgcggcc gagttgcgtg ccgagggttg cccggagcct ctggtgccgc
     6361 atcgatcgtt cgagggcaat cgcccgtcga acaccctggt gctcgagcgg ctgacaccgg
     6421 cggcgctcgg ccggctggtg gcgctctacg agcacaatgt cttcacgcag ggcgcgatct
     6481 gggggatcaa cagcttcgac caatggggcg tggaactcgg caaggcgctc gccagccgga
     6541 tcgtgccgga actcgaagcc ggcgtgcacc ctgcgggcca cgacagctcg accggcgcgc
     6601 tcatcgggcg ctaccggaga ctgcggggcc gctgatgcgg tgcgtgttct cgaccatcgt
     6661 cgtcgttgca tgagcatgcg cctcgaagcc atgccgaatt tccgcgatgc cggcggccat
     6721 gccacggccg acggccggcg catccgcacc ggcgtcctct accgctcgga ccagatcggc
     6781 aagctcagtg acgaagacgc ccggcgcctg tccggcatcg gcatccgctt cgtctacgac
     6841 ctgcgcaccg caggcgagcg cgcgaccctg tccaccgagc gtatccccgg cgcgacgcac
     6901 gtcgtgctgg acgtgctcgc cgacgatcgc cgcgccacgc cggcgcaggt gatcgcgctc
     6961 ctcggcaacg ccggagccgc cggcgacgtg ctgggcgacg gcaaggcggc cgcgctgatg
     7021 accgagagtt atcgatcgtt cctgcagctc gagagcgcgc gggcaggctt cggccagctg
     7081 taccgcgaac tcgcggacgc cgcgcgggtc cctgcgctcg tccactgcac cacgggcaag
     7141 gaccgtacag gctgggcctg cgcgtcgctc cagctgtggc tgggcgtgcc gtacgaggcg
     7201 gtgctgcagg atttcctggc gagcaaccgc tacatattgc cgaagtacga gccacacctg
     7261 cgggcgttcg cggtcgcggg aggcgatccc gaattgctgc tgcccgtgct cacggtgcgc
     7321 gaggaatacc tcgcggcttc gctgcacgaa atgcagcgga tgtacggatc gatcgacggt
     7381 tacttctccg cagggctcgg tctcggtccg gtggttcagc gcacactgcg cgagcgcttc
     7441 ctcgactctt cggcagacca tgacgcagtc aagcagggtc gccggacgct gccgtgaaca
     7501 ccgcgactcg acgcatactg atagcactgg tcgttgtact ggctggcgca ctggtctggg
     7561 ccgcgatcgc cgcgttgcgg ccgctcccgg agcgcagcct cacgatggcg accggaccgg
     7621 acgggagcgc ttaccgtttg tatggcgagc gctaccggac gctgctcgcg aagcagggcg
     7681 tcgaggtgcg gctcgtcgag acgagcggcg cacaggacaa tctcgcgcgg ctgctcgatg
     7741 cgaagtccgg ggtccaggcc gccttcgtgc aggctggctc gacgacggag gaccagtcgc
     7801 cagggctcgt gtcgctcggc acgatgttct acgaaccgct gtggatcttc agccggtccg
     7861 gctcggacca gctcgccctg catgacctgg tcggccgccg catttcgatc ggtccgcgcg
     7921 gcggcgcgac acatgagctc gcgctgcggc tcatgaggct gaacggcctc gacccggcgc
     7981 acctgcagct cgaggagtac ccgccggagc gggccgccga ggaactcgag agcggtcgca
     8041 tcgacgtcgc gatcatcgac actgcctggg actcgccggt ggtccgcggg ctgctggccg
     8101 acactggcgt cagcgtgcag gggtttccca gggccgatgc ctacatcgcg ctgctgccgt
     8161 acttgagcaa ggtcgtgctg ccgatgggtg tggcgagcct gtcggaaaac cgaccgccgg
     8221 ccgacgtcac gctgatcgca ccaaaggcga gtctcgtcgt gcgcgaggac ctgcacccgg
     8281 cattgcagta cctgctcatc gacgcggcaa tgaagatcca cggcgggccg ggcgtgttca
     8341 accgcgccgg cgaatttccc gctgccgaga tgatggacct gccgctgagc gaagaggcga
     8401 atcacgtgta ccgcagtggc ccgagcttcc tgcgcaggca cctgccgttc tggctcgcgg
     8461 agctggcgca gcgcctgctc atcctcttga taccgctcgc aggacttgtc tatccgcttt
     8521 cccggctggt gcccgaggcg tggcgctggg agatgggacg ccgcctgcga ggcatgtatc
     8581 gcgaactgct gcgcatcgaa caggcgctgc gccgggcgtc acccggcccg gagcgtgtcg
     8641 aaatgctccg aaagctggat gacctggagg tgcgcgcggc ggccattcgc cttccagact
     8701 cgttcgccgc acccgcctac gaactcaagc agaacatccg cttcgtgcag gaaaaatacg
     8761 cccgctgagg ggatgagggg acattctcgt attttcgccc atcgaaaata cgagaatgtc
     8821 cccgaatacg attacgccat tccggcggcg gcgagttgct gctcctgttc ggcctcgctc
     8881 agcttcgggc agtgccgcga gatgttgcag agaaacgcga tgagctgcgg cgcgtgcacg
     8941 ccgagcagca gcttgcccgt cgtgagcagc gacgccgaga tcccgcgctc cgggtcggcc
     9001 caggcgaaga tgttcgcaaa ccccaggtgg ccgaacgcct tcgaggtgtc cgggccgtac
     9061 aagccgaatc ggctcgagcc gagcatcatg ccggccgagt agcgcagcgg caccatcagc
     9121 gacttgtcga tttccggttt gccgacttcc gcgatggcgc gacgcaccgt cagcggctgg
     9181 aagacgcgcg tgcgacccag cttcccgccg tccaggaggc actggaagaa cttcgagcac
     9241 tcgtccgccg tgccaacgcc gttgcctgcc ggtatgaccg cccggtagta agcagccgtg
     9301 ttggacacct ccacggcgtg acgcagcgac gtgccgatcg cccgcttgag gtacatgtcg
     9361 gtgacgccag tcagccggaa gcccgacacg taattccgtg cgacgcggct gagggcgcgt
     9421 ccgcgcgcgc cgaaatcgag cgtggcgagt ccgagcggct cgctcacgtt gcggcggaag
     9481 aacgtccgga aattctcgcc ggaaaccctc tcgaccaggt cggcaatcac gaagcccgag
     9541 gtcagggcgt gataggcgtg gtgctgcccg ggtcggtccg gcgcggtccg gtagatgagc
     9601 cgcaggacct ccttggagtc gagcagcagc tgcgggacgt cgagtccttc cgtggggatg
     9661 gtggggatgc cggccttgtg gcacagcagc tggtagatcg tgatgtcgcg cttgccgttc
     9721 tgcccgaact cgggaatgta gtggctcacc gggtcgagca ggctcaggtg cttgcgctcg
     9781 gacagcaggt gcaccgtcat cgcggccatc gccttgctgg ccgagaagat gcagaccggg
     9841 gtctccggcg tcatggcgac cttcggctcg cccgcttcgc ccgggccgtt gcccctggcg
     9901 tgaccgatgg cgcgcttcag gacgatcctg ccgttgcggc gcaggaccac ggaaattccg
     9961 gggtgcatgc cggtcgcgta gagactctcg acggatttcc agatcgcatc gacgccctcg
    10021 cgcgtcatcc cgacaagggc aggattggac tcctcggcgg tcgccgcggt ggtcacgctc
    10081 gcgaggtcag cgggcacctc ggctccgtcg atcacaagcc gtcgcaacgc agctggtacg
    10141 atttgcatgg cgttcagcat cactggcctc ctgcggcctt gcgcctgagc accgcctcgc
    10201 gcaccaggcc aaggcggtcg ttgccgaaat acatgtcgtc gccgacgaag atggtcggtg
    10261 acccgaagcc accgcgcgat atgacttcct cggtgttcgc cttgagtgcc gccttgatct
    10321 ccggcgtggc gatgcccgcg aagaaacctt gtggatccgc gccgacccgt gcgcagattc
    10381 cggcgagcac cgcgtcctgc gagatgtcgc gatcgtgcgt ccagtacgcc tcgaacaccg
    10441 cctgcgcgaa gggcacgagc ttgccctccg cttcgagcag gatgcagccg cgcatcgcct
    10501 tgacgctgtt caccgggaag accgtcggcg gaaagacgat cttgagcccg gccagccgcg
    10561 cccagtcctg caggtccttc ttcaggtagg cctgcttggc gggcacgccg cgctcccgga
    10621 actcgtagac gctcgggttg accgcattga agatcccgcc caccagcacc gggcgccagc
    10681 ggatcgcgac gtcgagttcg cgggccatgg gctggatgtt gtggaaggcg agccacgtcc
    10741 acggactcga gcaatcgaag aagaattcga tcatgcttgc atgctgtcag aagccggcaa
    10801 ggccgtcact acgatgattg acctatacac cgccccgact ccaaacggcc acaaggtatc
    10861 gatcgcgctc gaggaactgg cgctgccgta caccgtgcac gtgatcaacc tgtacaagct
    10921 cgagcagcgg cgtccggagt tcctgaagat cagtcccaac ggccggatac cggcgatcgt
    10981 cgaccgggac ctcggcgact tcgcggtctt cgagtccggt gcgattctca tttacctcgc
    11041 cgagaagacc gggcgcctca tgccggccga cgtgaagggg cgttcgctgg tgatgcagtg
    11101 gctcatgttc cagatgggcg ggatcggacc gatgatgggg caggcgaacg tcttcttccg
    11161 ctatctcccg gagaagatcc agccggccat cgaccgctat cacgccgagg tgcgccggct
    11221 gttcgaggtg ctggacgccc gcctcgcgga ccacgaatac ctggcgggcg attactcgat
    11281 cgcggacatc gcgaactggg cgtgggtgcg cacgcaccgc tggtcgggcc tggaactcga
    11341 cgggctcgag cacctcacgc gctggaaacg ccagctggct gcgcggccgg cgtgccagcg
    11401 gggcatcgag atcccgccga atccgctcaa gggtgctgac gcaacggcgg ccaggaagtt
    11461 cgcggaggcc gcgcgccaga tggtcgtgac gggtcacgag cccacctgac ggcgggttcg
    11521 cggccggtcc acggaccgcg cgcggggccg tccgcgcatc ccggggttcg tcagcgcagg
    11581 cgcggcgcca cgtactcgtc cagcgaccag cgcccgggac cgaacacggc caccaccagg
    11641 agcaggctgc cccagaggta gtgctggccg agcgcgccct cgaatcccgg gctcagcagc
    11701 acgtgcgcgt acgagacgac cgccatcacg ttgacgaacg acagtccgat ggcggcgtat
    11761 cgcgacagca gcccgatgac caggagtgac gggaagagca gttcgccggt cgttccggcg
    11821 atcgcggcga tgcccggtgg cagcaccggc acgtgatact cctcctggaa caggtacagc
    11881 gtcgtgtccc acgtcgtgag cttgagccag ccggacttga ggaactgcca ggcgacgtac
    11941 cagcggatcg ccaggagcag caggtcctgg aacaccgccg ccacgcgcga cgcgaggtca
    12001 ttgagacgaa cgatgcttgg gccgccgatc atgcttcggc ccccccggta cacgccggag
    12061 ttgccgcggg ccgcaagcca accaccagcc catcctcgaa catccagtcg agcactccgg
    12121 gaagcgccgc gacgggcagg gtcgatgcgt cgagcgccgc gccgagagtt ccaccgtcgc
    12181 gaaggtggcg aatgaagtcg gccggttcgc gcggaaccga gcgcagcgcg agctgtcccg
    12241 cgatgcaggc gacgaccacg tgctgcgggc cagcggcgga gtccatgcct tcgacgtcgc
    12301 agccaggctg gtgcgcgcgc cacaccgacc agatcggatg gggcgaactc accactcgca
    12361 ccccgggctg tatttcgagt ccaatgtccg ccagctgctc aggaggcacg gacgcaagcg
    12421 cgtcgcgctg cagcggctgc atcgcgcgcg cgacgagtgc ttcctcgcag gcccactcga
    12481 gccgtgccag atcggcgagc cacgagtcgt cctcgtcgtc ggcgagccgc tcgtgcagcc
    12541 agtccggcaa ggcctgaccg acccagtgca ggtcgccgct gcgcgacggg tgggcgccgc
    12601 gatactcgcg cgcgagtgac tcgaaggttg cgtcgcccag cgtggccttg agcaccggat
    12661 aggtgcgcga caatgcgccg tagaacatcg cctgcacgtt gttgcgatag atccgcagtc
    12721 gcctcccggg ctcgagtccg tcgtcgacga tctgcgaggc catgtcgcga gccgcgtcgt
    12781 ccgcggcggt cccggtgcgc agcgcggctg cgaaccgggt caagagctcc tgcagcggag
    12841 caacttcagg ccgcgagttc accgcagcgc tccaggattg cgttggcctt ccgggcctcg
    12901 gcgagcagga cttcgaattc gggcaggtcc gtgtcccatt cgatcagcgt cggcacgcga
    12961 ccgagccggt cgatcgctcg cgcgtacagg gcccagacgt cttcgcacac ggccgaccca
    13021 tggtcgtcaa tgaggatgtc gcggcccccg accggattgc gccggtgccc ggccaggtgg
    13081 atctcctgca cggcggatgc cggcacgctg tcgatgaagc gcgcggcgtc gaggccgaga
    13141 ttcaccgagt tgacgtagac gttgttcacg tcgagcagca ggccgcagcc ggatcgttcg
    13201 gcgacggccg cgaggaattc ccactccgtg agccgcgatg ccgaatacgc gaaataggaa
    13261 ctcaggttct cgatgaggat ctgccgcccg aggaagtcct gaacttcgcg gacgcgcgcg
    13321 ctcaggtgct ctacggcttc ctcggtgtag ggcaggggca gcaggtcgtt gaaatgttcc
    13381 ccgcccgagt gtccccagca ggcgtgctcg ctcacgagcg caggctcgaa acggcgaacg
    13441 gcgcgcttga cgcgctcgag gtgggcgcga tcgagcgatt ccacggaccc gagccccagt
    13501 ccgaccccgt gcaggctcaa cgcatgggac tcgcggacgc gctcgagctc gtgcaggtgc
    13561 gggccgccgt cgtggaagta attttccgtg tgcacctcga cccacgcgac ggccggagtc
    13621 ccggccgcca cgtgggcgta gtgcggggag cgcaggccga tcccggcgct ggccgggatc
    13681 gacgcgccgg ggacccggct ggacaccgcg tccctcatga tgcgatcact tggcgggtgg
    13741 ggtggtcttg ccgccggcct tctcgcagtc ctccttggac atgtgcttcc actcgttcgg
    13801 gtcgttgtcc ttcttcgcct ggcccgcgca cgaatgcccg ttgccggcgc agtcgttctt
    13861 gcccgccttg gcgaccccat agcacttgtc cttgctcggc gtgtcgcccg cggcggcgat
    13921 gccgctggcg cccacggcaa ggaagctgcc cacggcagcg gcaatcgcga tcttcttgac
    13981 gtccatcgag tgttctcctc ctgcgtgaat gatgcgtatc ggcaccgcgc ccggatccgg
    14041 gcaggaaatg acggtgctcc ggccagtaga cccggcggcg gctgaaattg tttcacaggg
    14101 gggggcgcac aagaaaacgg cgcccgcgaa gggcgccgtc atggccggac ccgtaaatgg
    14161 tgtctgaccc caattccccc cgtttgcgcg gcggctgccg gccggctcgt cgcctgccga
    14221 cagcccccag cgttcagagc ggtgcgtcgc ccgggcagag cccgatcttg gcgccggccg
    14281 cgagcgcgat gtccatgccg atcgggaagt cgaccttgag agtcttcgcg tatcgccccc
    14341 ggccggcaca gacgaagacc tggtcgaggt cgatgcgata gccctggtag tagtcggcgc
    14401 cgagcggggc gccggtgccg atcagcgtct tgccgtcggc ggacaccttg gtgccgagga
    14461 acacccagtt cgtggcctcg agcacgccct gcgactgcag gaacgccgag agctgcatcc
    14521 agccaagctt cggcgtgaag atcgccccgt cgatggcgga agcattccag aagtcgttgt
    14581 agctggtctc gccggtgatg acccgcgcat cgtcggagat cgcgtacgcg atggtttggc
    14641 gccggttgca caccgtggtc tcggccccgg tgtcgtccca cgtcttgaag cagaacggct
    14701 tgcggtcgcc accgaggttg cgcgtgccgg cgtacggggt ccagatgaac gagtcgttca
    14761 ggtccgggcc cgcgttgtag cccaccgcga cgttgcccgc cgaattgatg gccagcacct
    14821 cgccgacgta gccggtcggg ttgtagtcgc tgacgctgcg aggctcgcgc aggatcgtct
    14881 gctcgctgcc ctgccagatc gacccgatgc ggtaggggaa cgtgtcgttc atgtcctgcc
    14941 agccgacgac cacgccgccg ttgccggaga cggcgttggc gcgcgcggag tcggtgccga
    15001 ccttcgacag cagcttcaca ccggtgcgcg cattccactt gaacccgtag acgtgcgtgc
    15061 aatccttgaa ggcgagtccc accgcagtgg agccgtcggt cgacaggtcg aagatcgacg
    15121 tgtggaagat gtcgcagccc ggccagttgt ccttcgtgat ggtcttccag ccgccttcct
    15181 tggtccagag cgcggcacgc tgctgcacga cgtcgcccca gctggtggtg acagtgggtc
    15241 cggcaaccgt gccaccgacg acccggccat tgccggacat cgcgatggtg acgccgctgc
    15301 cgccgatgaa cgtgacgtca ccggtcttcg cgaaactagt cttgtcccac acccagtacg
    15361 gcgactcgtt cgacggtccg tagattcccg cgactttctg gccattgtcg gaaacgacct
    15421 gcggcatgac gtacgggatg accgtgaagc ctggagcgcg cgcaatgccg ttgaccgcga
    15481 tggtgatctc gcgggtctcg ccggctgcca ccgagatcgg agtcgacgcg caggcgtcgt
    15541 ccttggaggc atcgccgctc tcgcccgcgt tccagtactc ctcgggcccg agcagcagcg
    15601 ccttcggcgt gctgtaggcg ccggcgcgca actggtcgat gtagagcagg tagttcacgc
    15661 cgggcacgag tcccgtcatc gtgaagctac cgtccgcgcc gagcaggccc tgcgtcgagt
    15721 cgccggaaat gcgcgagatc gcgccagtga gcggcttgtc cgcgcggcgc gcgatcacgt
    15781 tgatcccggt gagctggctc ttgccgtcct tcgcgacgat cctgccgtgg atggttccag
    15841 tcgtggcggc atatccggtc gctggataga tcgaagagag cgctgccatg tcgtccgcgt
    15901 cgtcgagcac cttggccatc tcggggctgt tgtaggtcgg gctctgcggg aacggatcga
    15961 tcatcgggta catggtctcg atctggttcg ccgccggata gcccgcggct gccggcccgc
    16021 actggtcggg gccagcctgt tcattgccgc cggcgtagaa cgaccacgac gtcgggtcgt
    16081 tcatctgcag gaacccgttg acctgcgtgt gcgccaggtt gatggcatgc ccgaactcgt
    16141 gcgtcacgac gcccgagatc tgctcggtgc tcgtcgcatt gaggaacgag ccaccgatga
    16201 cctgccagcc ctcgatgatc tgcgtcggat cggagtccga tgcgtactcc ggtgaagcga
    16261 tcccgagcac accgtagccg tcgccgccgg tgagcgcgtc gatgacactg ttgtcggcgt
    16321 cgtagatggt ctggatccca ccgccgttgt acgtgccgat gtaatccgac acgttggcgc
    16381 cggtaatgtc gactggcgac cggtccacga cctgcgcacg gaacgacgag gtcggcacgt
    16441 gcgaccactg gtacacggtc gcggccacga gatcgtcgcc ggccttctgg tccagcaccg
    16501 ggatcaccgg gttgccggcg ccatcgagca cacactcgta cgtcacgggg tcccgcacgc
    16561 agtcggtgcc gacgttgagg ttgccgctgt cgaggtggac cggcacggtg ccgacccagt
    16621 ggagcggcgt catcacgccg ttgaccggcg tgacgtagag cgggccgccc gccagcgccg
    16681 gcgcgatcga accggccgcg aggaaggcgg cgatgccgcc gcgcagtacg cggctcatcg
    16741 ggttagtgac gcgcattgga gagactcccg ttcttgaccc agttgccacc gaccgcacgg
    16801 tgcaggaact tgcggaacgt gtccgcatcc accgggccct tcttcgtgct gaacatctgc
    16861 tgctcgttcg cgtcgagcag gccagccttg gctcggacgc ccgagaacag gctggcattg
    16921 tcgttgctgt tcacggccac gccgttcgca acgctcagct tgccctggcc gaggcccacg
    16981 ctcgtctgca ggcccgtcgt gcgcgacttg ccgtagagga agacgatcgc cgactcgtcc
    17041 ttgcgccagg tcggccagcc ggtcgggcgg ccgagataga ttcggccatc ctccagcgtg
    17101 cgaggcttgt ccagcccgaa ttgccggaag gtgtaggtgc cggccgactg tccgcggatc
    17161 gtgtccgaca ccctgagcgt gacttcggtg tacggcaggc cgtgtgcatc gaacccgtcc
    17221 gtcacggact ggacctggcc caccacgatg gtgtccgaat ggccgagcag gtcaacgacg
    17281 gtcatcggga tgacgctggt cgcgtggctg gatgggctgg cggctccgag cgccagcgcc
    17341 gcgagggacg cgcgcgcgaa gacgcgcatc cgccggctga agtgactgga aatcatttga
    17401 cagaatccct ctcgaggatt gaaacgagcg tgcccggcgc gaagccgggc acgagtgggg
    17461 gcaagaatgg cgaactgggt cacgaattgc cctcctctcg ccgaggggga aacgcgaaaa
    17521 acccgtcccc cctccacggg aggcttgacc cagcggcggt gccgtgatct cggtgggcgc
    17581 gtcagcctgc accgcacgac ccgctcagcc ttgcagcctg tgcgcgccgc gtgcgctcca
    17641 cagccgaacg atcgacgccg ggggtcagtt ggcggtcggt gcagtcgcct tcggccgata
    17701 ccggtcgcgt aactccgtct gccgcgagcg catcgattgc gcctggccgc cgatccacac
    17761 catctcggct gccgtcgtca cctcgagggg atcgcccgac cagagcacga ggtctgcggc
    17821 gtagccgggc gcaatgcgcc ccagctgggc accgacgccg aagatctcgg caggtgcgct
    17881 ggtgagcccg gtgagcgcgg cgtcccaggg cagcccgtgc gcgacggcca cgcccgctgc
    17941 ctgccgcacc ttgcgcgcgt tctgcgtctc gccggtcgtg aaggcgacct tcacgcccgc
    18001 gcgatgcagc cgcgctgcgt tctccatcgt cgcgccgagc tggtcgaaat ccccgggcag
    18061 gtcgacgagc gggttcagca cgaccggcac ctgcgccttg gcgagcagcg cagccacctg
    18121 ccacgcctgc gcaccgccga cgatgaccgg gtgcacgcca tttcgcgcgg cgtaagccag
    18181 tacctggcgg atgtcggccg cgcgatcgac atcgaatgcc atccgtccgc cggtgaggta
    18241 gcgggccagc acctcgcggc cggtcggtgt cagcagtcgc atatcgccgg ccgcgagcgg
    18301 acgtcccggc agcgactcgc gcgtcgcctg gtcgagcagc atgaactgcg ctgcgcgact
    18361 tttgccggag ccctcggtcg cgttgccgcc gaggtcgatg aacagcgtcc ggctggcggg
    18421 gaggaacgcg tcggcgcgac cgtccagtcg cgcaatcgat ccctggccag cgaacatggt
    18481 tccgtcgggc aacgccgtcg gcgcgaccat cgtgtaggtg atgccctcga cgcgattcac
    18541 gccgatggcg accgagtctg cattgaatgc gggactgacg tcgaactcgg gacgcggctg
    18601 gaactcgggg ccgggcttgc cgtcaccgta cgaaaccgac gagtccaccg tcgacggctc
    18661 ggccgacact tcctcgatgc cgagtgcggt gattccaccg aacaggcccg gcgtgagcgg
    18721 gcggccgttg gcttcgacga ccgaggcgcc gggaccggcg gcgagtccgc taccgacggc
    18781 gacgatccgg ccgtcgcgca ccagcacgtc cgcccgctcc atcgtcccgc gctcggtttc
    18841 ggtgtgcacc tgcgcgccgc ggatcagcac gtcctgcgcg tgtgccgcgg aaaaagcgac
    18901 gaggcccgtg aggacgagca ggccacgatg cgcccggaac agggtgcggc ggctcatcgc
    18961 gcgcctcccg tcggctggcc gagcaggaag tcggaggctg gggccggcgg cggcgacttg
    19021 cggtcgagca ccacggcgcc gtcgatgtag acgaggtccg caagtgcgta gacgctgaac
    19081 ggattgctgt tccacaccac cacatccgcg gcacgccctg gttcgagcgt gccgacacga
    19141 tcggcgattc cgagcgcccg ggcggggttc gacgtgagcc agcggatcgc ccgctcgggc
    19201 gggatgtcga gcccggcgcg ctggccgcgg gacatcgcct tcgccgcctc ctggttcagg
    19261 cgctggatgc cttcctcgga gtccgagtgc acgatcgcgc agccattcgc gggtgcgtcc
    19321 acgaatgcga ggttctcctg gatgccgtcg tacgcctcca tcttgaagcc ccaccagtcg
    19381 gcccacagcg cgccgcagat gccttcctgc gcgagccggt cggcgatctt gtacgcctcg
    19441 acgccgtggt gaaaggcggc gatcctgaaa ttgaactcgt gcgacaggtc gatcatcgtc
    19501 gccatctcgt cggcccggta gcagtggatg tggacctgca gctcgccgcg catggccgcg
    19561 gccagggttt cgagtcgcag gtcgcgcttc ggctgcttgg gcggcccctc gtccttgccg
    19621 tcgtccttca ccttgccctt ggcctgcttg ccctcgtact cggcgagttc ccgcgcgtac
    19681 ttgtcccact gccgctggta gtcctgcgcg tccgcaaacg cctgccggta gccctcgaca
    19741 ttgcccatgc gggtcgatgg tgctcgcccc ttgttcccgt acacgcgctt ggggttctcg
    19801 ccgcaggcca tcttgaggcc ttgcggcgcg cccgggaatt tcatcgcctg gtaggtcgtc
    19861 gcatggacgt tgcgcagggt gaccccgcgc ccgccgatca ggttcgcgga tcccggcagc
    19921 acctgcaggg tggtcacccc tccggcgagc gcggtcgaaa aaccggggtc ctgcggccac
    19981 accgagtgtt cggcccagac gccggccgtg accgggtccg tcgcctcgtt gccgtcctgc
    20041 gtcgcgcgca cgccgggact cgcgtacacg ccgaggtgcg agtgcacgtc gatgatcccc
    20101 ggcgtgatcc agcgaccggt tgcgtccacc acgaccgcat cgcctggatc gaggcccggg
    20161 ccgaccgcga cgatcttgcc gtcgcggatc agcacgtccg ccccatcgag gcgcttgccg
    20221 gtgccgtcga gcacggtcgc gttgcgcagg agcgtcggtg cgccgggatg cgccacgtag
    20281 gtggaggagt agggatcgac atcggcgagg gcgggggcgg cgattgccgc aatgcctgca
    20341 agcaggaaac tcctcaagac cacgtgcact ccccccatgc gcaaggtccg cgaaggatgt
    20401 cgggaccgcg gcggcgtgtc cagaggttcc cgaccttggt acccgggtcc gccgctggcg
    20461 cgtggatcgg ctacgatcag tggcactgca cgaagggatc cggaggccac gccatgcgac
    20521 tccatgtcga cgtgcgacac gcccctgtgg cgattgccgc gtcgctgctg ttcgtcggag
    20581 gatgcgggga gaaggcaccc gagcccgctc ccgtggccca ggcggggcca ccaccgacac
    20641 cgaccgtcca gcaactgaag tcggcgaccg tgtccggcgt gttcggcgaa cccgtcgcgc
    20701 tcgcggacgg caagtacgac ggaccgccgc cgacaccggg cgcggcatcg cacccgacag
    20761 ccttgctttg ggacagcgcg gtcgtattcg gtgacgtcga cggtgcgccg ggcagcgaag
    20821 ccg
//
LOCUS       DAOMFD010000016        20422 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_472_length_20422_cov_31.957529, whole genome shotgun sequence.
ACCESSION   DAOMFD010000016 DAOMFD010000000
VERSION     DAOMFD010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20422)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20422)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20422
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_472_length_20422_cov_31.957529"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1608
                     /locus_tag="PL152_01955"
     CDS             <1..1608
                     /locus_tag="PL152_01955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0009290 - DNA import into cell involved in
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HQR48067.1"
                     /translation="LARSGTSHLMAISGMHIGMFAAVAAWLAAAVQRWRQRRGALGTR
                     RDSAVLVGTAAAFGYSLLAGWSVPTQRTMIMIALVALALRLRRRLGPGDALAAGAIAV
                     LALDPLAPLAPGFWLSFGAVASILLATMGFAGSPGLVAGFARTQLAVTLGLVPVLIGS
                     FGSVSLVSALVNVLAIPLYTLVIVPAVLVATGLAALLPDAGAAALHAVAWLIEATWPL
                     IAAPAAWPLATWGVAALPAPLWIALVAGATAAVAPLPVPGRVAGGLIVLALCAWRPVP
                     IDTGAVRFALLDVGQGLASVVETRTHVLVFDAGPDFRSGTDTGVLVVEPYLRSRALRR
                     VDLLVGSHDDADHVGGADSLARLTRVLHMAASGRALDRLGRVESCRVGRRWTWDGVRF
                     EWLHPAMPLLPRDNDRSCVLLVRAGEHSLLLTGDVEQLAEQQVLRTGLTGHPDVLVVP
                     HHGSRSSSGPAFVAATQPRWALVSSGYHNRWGFPAPVVVERWRSSGAQVLDTASSGAI
                     ELDMRPGRPLAAPIEWRKSHPRIWKDP"
     gene            1684..2325
                     /locus_tag="PL152_01960"
     CDS             1684..2325
                     /locus_tag="PL152_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR48068.1"
                     /translation="MFEIVKAGGIVMVPIIFCSILAVAITLERLWTLREQRVTPAALT
                     DKVWQWVENRTLTDKQIAALEAHSPLGRILAAGLANRHRDRTVMIGAIEDAGRHVVHD
                     LERFLNMLGTIAAISPLLGLLGTVTGMIRTFKAITLSGVGNPTAMAGGIAEALITTAA
                     GLLVAIPALVAYRYLRGRVDSLVIQMEKESLKLVQAIDRTSGTTPLAVQEEAA"
     gene            2322..2759
                     /locus_tag="PL152_01965"
     CDS             2322..2759
                     /locus_tag="PL152_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR48069.1"
                     /translation="MKFQPRRTEEPELNMTSLIDVVLLLLIFFMLSTKFIDEGRLQIR
                     LPSAGVAPDAESTRDAIEIAVTAEGGYRVNGRPLINNSPETLATALGRVAQGNRGMPV
                     TIRADARAMHQSVVTAMDVAGRAGYRQINIATVNDEPQGTAGH"
     gene            2731..4497
                     /gene="msbA"
                     /locus_tag="PL152_01970"
     CDS             2731..4497
                     /gene="msbA"
                     /locus_tag="PL152_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR02203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005319 - lipid transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006869 - lipid transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A export permease/ATP-binding protein
                     MsbA"
                     /protein_id="HQR48070.1"
                     /translation="MSRKAPRGTELKMPPNALRTYRRLLGYLGPHRTMFMVGVLGMVM
                     SATSDAAWAGFIKFFLDGTFVNKDPRMVWLVPVVLIALFAFRGVGDFLQTYVPGFVGR
                     HIVKTMRLQLFDKYLHLPVSYFDRESSGVALSRLTYNTEQVAQATTESITVFVRDSLT
                     IIGLVGYLLWQNPRLTLLSLTVGPLIAFMVRRVNLLFRRYSRRIQNSMGDVTRVAKEA
                     IEAPRVVRVFNAQEYEAQLFEAVAEHNRRSYMRLMLTKGLSNPVVMLVAATAMAGVLY
                     IATVEAMKGSMTVGDFTSYIAALGLVSAPLRRIVNVAGPLQQGIAAAQSLFQVIDARV
                     EDTGGDRTITRARGDIEYRDVCFRYPSGPDDVLRDVSFKVRAGETLAIVGRSGSGKST
                     IVSMLPRFYDVSAGSVMLDGHDVREYGRKDLRAQMSLVSQDVVLFNDTVRRNIAFGFP
                     AGDAEIEAAARAARVTEFTESLPDGLDTIVGDRGMLLSGGQRQRIAIARALLRNTPVL
                     ILDEATSALDTELERQIQEQLDQLMHNRTTLVIAHRLSTVENANRILVMDGGRVIEEG
                     THEELLAREGQYAVLYRMQFNE"
     assembly_gap    4501..4600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4649..5689
                     /gene="lpxK"
                     /locus_tag="PL152_01975"
     CDS             4649..5689
                     /gene="lpxK"
                     /locus_tag="PL152_01975"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HQR48071.1"
                     /translation="MTVGPGLQGLWYGPAWRTVLLWPPALLFGVAVAARRTLYAAGVL
                     RRSRVDVPVVVVGNLTVGGTGKTPVAAWLARQLTLRGHRVGVVLRGYGGRDSRRVRVV
                     EPGDSPRDVGDEALLHAAGGPHVVVIGADRVAAARMAADRGAAIVVCDDGLQHLRLAR
                     DYEIAVVDADRGFGNGWMLPAGPLREPTGRLESVDAVVRTWRSEGSDTPVHPRRPFVL
                     DARLSIGAAVNLASGERCDLGRFRGGRVHAVAGIGNPQAFFAALVAAGLDVEGHALPD
                     HAAIIPTQIGLAAGATVLMTAKDAVKCRECAEPGWWYVELDVCVERDAAGELLAMILD
                     RAGLTGAGAQIG"
     gene            5682..5870
                     /locus_tag="PL152_01980"
     CDS             5682..5870
                     /locus_tag="PL152_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008375064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HQR48072.1"
                     /translation="MDSKLLDILACPVCKGPLQWHRDVPCLVCRADRLAFPVRDGIPV
                     MLEEEARRLDQDDALLKD"
     gene            5873..6673
                     /gene="kdsB"
                     /locus_tag="PL152_01985"
     CDS             5873..6673
                     /gene="kdsB"
                     /locus_tag="PL152_01985"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HQR48073.1"
                     /translation="MAFKVVIPARYASTRLPGKPLADIGGQAMIQRVVQGACASGAAQ
                     VVVATDDERVASAVRDPAGKGRVTALMTDTALPSGTDRVAAVAERLAWGEDTIVVNVQ
                     GDEPFLPAALIDQVAGLLARDATAGIATLATPVRSLEEFLDPNVVKVVAADDGAALYF
                     SRAPVPWTRDGAMAGLGSQREFGGASRHIGLYAYRVATLKRITRLPPSRLEWLEKLEQ
                     LRALQSGVRIVVAECSVAPGPGVDTQADLERARQRAAGLNGDSGGGKT"
     gene            6670..6990
                     /locus_tag="PL152_01990"
     CDS             6670..6990
                     /locus_tag="PL152_01990"
                     /inference="COORDINATES: protein motif:HMM:NF019214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase N-terminal
                     domain-containing protein"
                     /protein_id="HQR48074.1"
                     /translation="MNQVLVTAFGITLTPWKLVGYLGVALFAGRWFVQMWYSRSHGRP
                     VVPTVFWLMSISGSLLLLAYFTFGKNDSVGILSNLFPAGVAGYNLYLDLAHKRSLREP
                     SGAG"
     gene            complement(6974..7681)
                     /gene="rluF"
                     /locus_tag="PL152_01995"
     CDS             complement(6974..7681)
                     /gene="rluF"
                     /locus_tag="PL152_01995"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HQR48075.1"
                     /translation="MRINKYLSETGACSRREADQWIEAGRVTVDGVKAVLGTQVAEGQ
                     AVLVDGQPLRARPKRVYLALNKPVGIECTTDREVRDNIVDFVAHRERVFPIGRLDKDS
                     EGLILLTNDGDVVNTVLRAEHQHEKEYVVAVDRPLTAEFLAGMAAGVPILGTVTNPCK
                     VTQVGRNTFRIVLTQGLNRQIRRMCEHFGYTVRSLQRVRIMHIRLGDLPLGRWRNLTP
                     EELRGLVRSPRTLSLRR"
     gene            complement(7688..8053)
                     /locus_tag="PL152_02000"
     CDS             complement(7688..8053)
                     /locus_tag="PL152_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014070492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor Sui1"
                     /protein_id="HQR48076.1"
                     /translation="MSTSRLVYSTSGGKLCPECARPIADCRCRRSKPAVGSAAPRGDG
                     IVRVGRETKGRKGKGVTVVTGVPLSGDALDELATRLKKRCGSGGTVRDGAIEIQGDHR
                     DVLVAELGTLGYTVKRAGG"
     gene            complement(8064..9257)
                     /locus_tag="PL152_02005"
     CDS             complement(8064..9257)
                     /locus_tag="PL152_02005"
                     /inference="COORDINATES: protein motif:HMM:NF019607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HQR48077.1"
                     /translation="MKRRATAVIIVAVAAVAAVYALLRLGPWRVGDPLLLLDMIVGRG
                     IASPSETTVAQQLVAASGFEVRIYEGGVTAARWLAMTPAGDLLVAQSRVGNVQILARD
                     ANGDGRPDRRRILLDGLDYPHGLAVHDAWLYVAERTAVGRIRFDAATGTTSGSYAHVI
                     TGLTGDGNHVTRTIGFGPDGKLYLSQGSTCNVCEERDHRRATMMRFDPDGSHAEIHAT
                     GLRNSVGFDWAPWDGALYATENGRDLLGDDFPPDELNRIERGRFYGWPYVNGYNVLDP
                     DLGHGHEALLAQSRPPVHGFRAHNAPLGIVFLRSPSRPPGYERAAIVALHGSWNRSVP
                     DGYKVVSLHWRDDGSIEERDFLTGFLSSSGVIGRPAGVAEGSDGAVYVADDYAGAIYR
                     VAPLR"
     gene            9456..10652
                     /locus_tag="PL152_02010"
     CDS             9456..10652
                     /locus_tag="PL152_02010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQR48078.1"
                     /translation="MRRAAIVSPLRTPVGAFGGSLRGVPVETLGAAVVKAILERTGLD
                     PARIEDVVFAQSYANGETPCTGRWIALEAGLPIEVPGMQLDRRCGGGLQAVVTAAMMV
                     QSGAADVVLAGGVESMSNVEYYTTDMRWGTRAGSVKLHDRLDRGRERSQPEHRFGRIS
                     GMIETAENLAREHGITRAAADEYAALSQQRAAAAWAGGRFDAEVVPLHVPQKKGDPLV
                     FARDEGIRAETTPEALARLRPIMPGGTVTAGNSSQQNDAAAACLVVAESLLPTLGLQP
                     AGFLVGWAAAGCHPATMGIGPVPAVRRAFEQTGLSFDRMDLVELNEAFACQVLAVLKC
                     WNWHDPGRLNVNGSGISLGHPIGATGARILVTLLHELGRRKGRYGLETMCVGGGQGMA
                     AIFERA"
     gene            10649..11164
                     /locus_tag="PL152_02015"
     CDS             10649..11164
                     /locus_tag="PL152_02015"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HQR48079.1"
                     /translation="MKAGRRMESTGKHRVLFVCMGNICRSPTAEGVFRRLVQERAADL
                     DIEIDSAGTHDYHVGEPPDRRAIAAALRRGIDIRQLRARTVEAADFERFDLLIAMDRL
                     NREVLLERSPPQFHARIRLFLEFAPDAHEEDVPDPYYGGAVGFERVLDLAEEASLGLL
                     DEVMSRAGRRR"
     gene            complement(11178..13856)
                     /locus_tag="PL152_02020"
     CDS             complement(11178..13856)
                     /locus_tag="PL152_02020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HQR48080.1"
                     /translation="MKRMLVNATQEEELRVALVDGQRIYDLDIEIPSRETKKANVYKG
                     KITRVEPSLEAAFVDYGAERHGFLPLKEISRDYFKARDGGGEGRPGIRDLIEEGQELI
                     VQVEKEERGNKGAALTTFVSLAGRFLVLMPNNPRAGGVSRRIEGDDRDQLREAMDSLQ
                     IPDGMGAIVRTAGVGRTAEELQWDVNNLVDIWNAVKQASEGRPAPFLIYQESKAILRA
                     LRDYLSDDIGEILVDKQEIYAEAQDYMQRFMPGNLRKLKFYDDHVPLFTRYQIESQIE
                     SAYSHKVNLPSGGSIVIDHTEALVSIDINSARSTRGTDIETTALNTNLEAADEIARQM
                     RLRDVGGLIVIDFIDMESQKNQRAVEDRLRDAVKQDRARIQIGRISRFGLLELSRQRL
                     RPSISESTNIVCPRCNGMGVIRSVESLALAILRLIGEEARKDRTAKVIAQLPVDVATY
                     LMNEKRDWLHGIETKSEVDVILVPNRYLETPAYELRRVRDDEVDLPEFSQISHQMAVQ
                     PTIDLEAIAAEEKAPPPPPVPAVTTVVPSTPAPPPAPEPVAVAAPPAPAVAVAPQFGP
                     RDGAFVRLWRWLFGDKRAPEPVAESSRGRRDHRDRDRDRDRERDRDRHRSHDRDARRG
                     RDERGRDARRGEQRGEQRSDRNDRPAARRDERRDAPRGEQRRDERGRRPDQPQSQGGG
                     QRDRRPDGQSQRQGQPQGQPQAQPQRSEPVQQSRPDQGQAQQRSEGQPPGEGQRGEQG
                     ARPDGERGERSGRRSRRGGRRRRREGGGEAFGAPEQRGDAAGADGTFDDSPRVEGARP
                     EGERWTPPVAAPEAAPARPATPPAAPVAPPAPAYAPPAGETSAPRSGSFDFEHRPEPV
                     PYSPPSPPVVRPPHDVQEWTPAPQNDSTRETPRNEP"
     gene            14240..15286
                     /locus_tag="PL152_02025"
     CDS             14240..15286
                     /locus_tag="PL152_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015671944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HQR48081.1"
                     /translation="MGYDNNATAPDQPGRPGVGVRYVEAGEGDDGQRLDNFLVRVLKG
                     VPRTHVYRLLRKGEVRVNSKRAKPDQRVAAGDRIRIPPVRQAETPAAAARAPSRSLQD
                     LITHSIVYEDDDLLVLNKPAGVAVHGGSGMAHGVIEVLRAARPEARELDLVHRLDRDT
                     SGCLIVAKRRAALRDLHAQLREGTTEKLYLALVCGKWNLGQKRIELPLATGERRGGER
                     HVAVRGHGQVAVSTFRPVQFFGNVATLMEVAIDTGRTHQIRVHAAYAGHPVAGDDKYG
                     DREKNAALRQYGVNRMFLHAASIGVNRPGSREPLHVSAPLGEDLRAVLDALVKAPGSR
                     RGAAQRRARSTAGR"
     gene            complement(15222..15815)
                     /locus_tag="PL152_02030"
     CDS             complement(15222..15815)
                     /locus_tag="PL152_02030"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase"
                     /protein_id="HQR48082.1"
                     /translation="MSVAPAPRLILASTSPYRRALLARLRLDFECIAPDVDESRQPAE
                     PPLELAQRLARLKAESVATARAGAVVIGSDQVAVLGDDVLGKPGTTENCIEQLHRSSG
                     REVVFLTAVHVVSGVTVSAESHVDRTVVRFRALGDGEIRRYVATENPVDCAGGFKAES
                     LGISLFDRIESGDPTGLTGLPLIWLCAALRRAGIPVP"
     gene            15913..16434
                     /locus_tag="PL152_02035"
     CDS             15913..16434
                     /locus_tag="PL152_02035"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HQR48083.1"
                     /translation="MSPARSDQVNAVELAGRAATLEREFDLTQLPRLVEAGALAGTRV
                     HAVMAFSKFEGRPMVSVRVDGRVMLPCQRCLAPSECRIDDASQLVIVADDTADVPGGF
                     EAAIGDVEHLSVAELVEEQVLLGMPLVPLHEDESQCGPSAAPQAVVEPGSAAEEMQRP
                     FSNLRELLDQGER"
     gene            16456..16650
                     /gene="rpmF"
                     /locus_tag="PL152_02040"
     CDS             16456..16650
                     /gene="rpmF"
                     /locus_tag="PL152_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HQR48084.1"
                     /translation="MAVQKSRKTPSKRGMHRSHDFLVKPALSVDRTSGETHLRHRVTP
                     DGFYRGKKVIEKPAAEEGQE"
     gene            16667..17701
                     /gene="plsX"
                     /locus_tag="PL152_02045"
     CDS             16667..17701
                     /gene="plsX"
                     /locus_tag="PL152_02045"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HQR48085.1"
                     /translation="MTAGLVIAVDAMSGDHGAEVTVPAALDVLTAIPDLRIALVGRGD
                     VVRPLCGTFVPERCSIVEASEVVGMDERPQDALRRKKDSSMRVAINLVEKGEATACVS
                     AGNTGALLATARFVLGMIPGIDRPAIVSAVPAAHGHTVMLDLGANPDCSADHLVQFAV
                     MGSVIAADLHGTERPRVGLLNIGEEDIKGTDAIRDAHKRLAAAPINYVGFVEGTDIFS
                     GDVDVVVTDGFTGNVALKSMEGLARFISGVMREEFTRTPVRKMSALAATPVLNALKVR
                     LDPGAHNGASMVGLSGVVIKSHGGANRAAFANAVRVAIAEARRGVPAQIGELMSRVAA
                     TAQPAASPHG"
     gene            complement(17710..18231)
                     /locus_tag="PL152_02050"
     CDS             complement(17710..18231)
                     /locus_tag="PL152_02050"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HQR48086.1"
                     /translation="MTYRLTIELSPRDVRHFRRELRKARAAVRIADDDEIVAAARELI
                     ATLRGSSLPDFVTGRLSKLEAMLAMVTDADWTLTDAERGPVLAALAYLCDPDDLIPDS
                     IPGIGLLDDAVMIELVFRELRHEIEAYEDFVRYRRSLSRRKADAEKLRRRRAQLVTRM
                     NRRRERERRSGER"
     gene            18320..19294
                     /locus_tag="PL152_02055"
     CDS             18320..19294
                     /locus_tag="PL152_02055"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQR48087.1"
                     /translation="MKRLHMLVRPRSAWIGWLAAASAALACADAGAAEYVLPANGDNV
                     IGRNSTAIAAREDTLFDIARRTGAGYEEMVAANPGVDPWLPGDGTEVLIPGRYILPDA
                     PRKGIVVNLPEHRLYYYPPPKKGEPAVVRTYPISTGKMDWKTPIGVTQIQSKQEKPNW
                     YPPASVLKEHEERGDPLPKVVPPGPDNPLGEYAMRLAIPGGAYLIHGTNKPAGVGMQV
                     THGCIRMYPEDIAELFPLVPVRTQVNLIDQTNKVGWQRGVLYVERHAPLEGTDNPADM
                     DPSVMDQLVSAAAAGRPVEIDWALVQRTFEQATGVPVPLSVPVRAAAR"
     gene            complement(19398..19712)
                     /locus_tag="PL152_02060"
     CDS             complement(19398..19712)
                     /locus_tag="PL152_02060"
                     /inference="COORDINATES: protein motif:HMM:NF023267.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine-zipper protein"
                     /protein_id="HQR48088.1"
                     /translation="MKTAIAKVLTVAAAAVALAGCVSQSKFDEYVAQNDAAVKAAQQE
                     AAAASATAKSAADASKAAADAANMANNTANQAGQAAKAAQACCDANSEKIDRAFQKSM
                     GK"
     gene            19962..>20422
                     /gene="lexA"
                     /locus_tag="PL152_02065"
     CDS             19962..>20422
                     /gene="lexA"
                     /locus_tag="PL152_02065"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008938833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HQR48089.1"
                     /translation="MDDLTPRQRQVLRFIQDAVSEHGMPPTRAEIAEALGFKSANAAE
                     EHLRAMQRKGVLELRPGASRGIQLKDSLRDQIGLPLIGRVAAGRPILAEENIEAHYQI
                     DPQLFQPRPHYLLKVRGMSMKNAGILDGDLVAVHRTPEVRNRQIVVARLEN"
BASE COUNT         3026 a         6845 c         7230 g         3221 t
ORIGIN      
        1 ctggcgcgca gcggcaccag ccacctgatg gcgatctccg gaatgcacat cggcatgttc
       61 gcggcagtgg cggcctggct ggcggcggcc gtgcagcgct ggcggcagcg gcgcggagca
      121 cttggtacgc ggcgtgactc ggcagtgctg gtcggcaccg cggcggcctt cggctattcg
      181 ctcctggccg gctggtcagt cccgacccag cgcaccatga tcatgattgc actcgtcgcg
      241 ctggcgctga ggctgcgtcg ccgtctcggt ccgggcgacg cgctcgccgc cggcgccatc
      301 gcggtcctgg cgctcgatcc gctggcacca ctggccccgg gattctggct ttcgttcggc
      361 gcggtcgcct cgatcctgct ggcgaccatg gggttcgctg gaagccccgg cctcgtcgcg
      421 ggatttgccc gcacgcagct cgcggtcacc ctgggcctgg tcccggtgct gatcgggagc
      481 ttcggcagcg tgtcgctcgt atcggcgctg gtcaacgtgc tggcgatccc gctttacacg
      541 ctcgtcatcg ttccggcggt gctggttgca accgggctcg cggcgctcct gccggacgcc
      601 ggcgccgcgg ccttgcacgc ggtcgcgtgg ctcatcgagg cgacctggcc actgatcgcg
      661 gcacccgcgg cctggccgct ggcgacctgg ggagtcgccg ctctcccggc gccgttgtgg
      721 atcgctctgg tcgccggcgc caccgcggcc gtcgcgccgc tgcccgtgcc gggacgggtc
      781 gccggcgggc tcatcgtgct ggcactgtgt gcgtggcggc ctgtgcccat cgacacaggc
      841 gccgtccggt ttgcgctgct cgatgtgggg caggggctgg cgtcggtggt cgagacccgg
      901 acgcacgtgc tggtattcga tgccggcccc gactttcgca gcggcacgga caccggagtg
      961 ctggtggtcg agccgtacct gcgcagccgc gcattgcgcc gggtggacct gctggtcggc
     1021 agccatgacg atgcggacca cgtcggtggc gcagattccc tcgcgcgcct cacgcgggtg
     1081 ctccacatgg ccgcaagcgg gcgggcgctc gatcgcctcg gtcgcgtgga atcgtgccgg
     1141 gtcggacgcc gctggacctg ggacggcgta cgattcgagt ggctgcatcc ggccatgccc
     1201 ctgctgccca gggacaatga ccgatcgtgc gtgctgctcg tgcgcgcagg ggagcattcg
     1261 ctgctgctca ccggcgacgt cgagcagctg gccgagcagc aggtgctgcg gactggcctg
     1321 accgggcacc cggacgtgct ggtggtcccg catcacggca gccgcagttc ctcgggtccg
     1381 gcattcgtgg cggcgacgca gccgcgctgg gcgctggtat cgtcgggcta tcacaaccgc
     1441 tggggctttc ccgcgcccgt ggtggtcgag cgctggaggt cgtccggcgc gcaggtcctc
     1501 gacacagcgt cctccggagc catcgaactc gacatgcggc cggggcggcc gctggccgca
     1561 ccgatcgaat ggcgcaagtc gcatccgcgg atctggaagg acccgtgacc gagtctggtc
     1621 gcagcctgcg cccccgatca agtatgatcc gcgggcgcag cgacagggga ccagggcgag
     1681 tacatgttcg agatcgtgaa ggccggcggc atcgtgatgg tgccgatcat cttctgctcc
     1741 atcctcgccg tggccatcac gctggagcgg ttgtggaccc tgcgcgagca gcgggtcacg
     1801 ccggctgcac tcaccgacaa ggtctggcag tgggtcgaga accgcacact gaccgacaag
     1861 cagatcgcgg cgctggaggc gcattccccg ctcggccgga tcctggcggc cggcctggcg
     1921 aaccgccacc gcgaccggac ggtcatgatc ggggcgatcg aggacgccgg gcgccacgtc
     1981 gtgcacgacc tcgagcggtt cctgaacatg ctgggcacga tcgccgccat ctcgccactg
     2041 ctcgggctgc tcggcacggt caccggcatg atccgcacgt tcaaggcgat cacgctttcg
     2101 ggcgtcggca atccgacggc gatggccggc ggcatcgccg aagcgctcat cacgaccgcc
     2161 gcgggactgc tggtcgcgat cccggcgctg gtcgcttatc ggtacctgcg cgggcgcgtc
     2221 gactcgctcg tgatccagat ggagaaggag tccctgaagc tcgtgcaggc catcgaccgc
     2281 accagcggca cgacaccgct ggcggtgcag gaagaggcgg cgtgaagttc cagccgcggc
     2341 gcaccgagga gcccgaactc aacatgacgt cgctcatcga cgtcgtgctg ctgctgctga
     2401 tcttcttcat gctgtcgacg aaattcatcg acgagggacg cctgcagatc cggctgcctt
     2461 cggcgggcgt ggcgccagat gccgagtcta cgcgtgacgc aatcgaaata gcggtcaccg
     2521 ccgagggcgg ctaccgcgtc aacggccgcc cgctgatcaa caacagcccg gaaacgctgg
     2581 cgaccgcgct cggccgggtg gcgcagggca atcgcggcat gccggtgacg attcgcgccg
     2641 acgcgcgggc catgcaccag tcggtggtga cggcgatgga cgtcgcgggt cgggccggct
     2701 accggcagat caacatcgcg acggtcaacg atgagccgca aggcaccgcg gggcactgag
     2761 ctcaagatgc cgccgaacgc gctgcggacc taccgcaggc tgctcggcta cctgggcccg
     2821 catcgcacca tgttcatggt gggcgtgctc ggcatggtga tgtcggcgac atcggacgcg
     2881 gcctgggcgg gattcatcaa gttcttcctg gacgggacct tcgtcaacaa ggacccgcgc
     2941 atggtctggc tggtgccggt cgtgctgatc gcactgttcg cgttccgcgg cgtgggggac
     3001 ttcctgcaga cctatgtccc cggtttcgtg gggcgccaca tcgtcaagac gatgcgcctg
     3061 cagctcttcg acaagtacct gcacctgccg gtgagctatt tcgaccgcga gtcctcaggc
     3121 gtggcgctgt cacgcctcac ctacaacacc gagcaggtcg cccaggcgac gaccgagtcc
     3181 atcacggtgt tcgtccgcga ctcgctcacg atcatcgggc tggtcgggta cctgctgtgg
     3241 cagaacccca ggctcacgct gctcagcctg acggtcggtc cgctcatcgc cttcatggtc
     3301 cgccgcgtca acctgctgtt ccgccgctac agccggcgca tccagaattc catgggcgac
     3361 gtcacgcgag tggcgaagga agccatcgag gcgccgcgcg tggtgcgcgt cttcaacgcg
     3421 caggaatacg aggcgcagct cttcgaggcg gtcgccgagc acaaccgccg ctcctacatg
     3481 cggctcatgc tcaccaaggg cctgtcgaac ccggtcgtga tgctggtcgc agcgaccgcg
     3541 atggcgggtg tgctctacat tgcgaccgtc gaagcgatga aaggcagcat gaccgtcggc
     3601 gatttcacct cctacatcgc cgcgctcggg ctcgtgagcg caccgctgcg acgcatcgtc
     3661 aatgtcgcgg gcccgctgca gcagggcatt gccgcggcac agagcctgtt ccaggtgatc
     3721 gatgcacggg tggaggacac cgggggcgac cggacgatca cccgcgcgcg cggcgacatc
     3781 gagtaccgcg acgtgtgctt ccgctacccg tcgggcccgg acgacgtgct gcgtgacgtc
     3841 agcttcaagg tgcgggccgg cgagacgctc gccatcgtcg gccggtccgg cagcggcaag
     3901 tccaccatcg tcagcatgtt gccgcgcttc tacgacgtgt cggccggcag cgtcatgctc
     3961 gacgggcacg acgtgcggga atatggcagg aaggacctgc gcgcgcagat gagcctggtg
     4021 agccaggacg tggtgctctt caacgacacc gtccgcagga acatcgcgtt cgggtttccg
     4081 gccggggacg ccgagatcga ggcggcggcg cgcgccgcgc gggtcactga attcaccgag
     4141 tccctgcccg acggcctgga tacgatcgtc ggtgatcgcg gcatgctgct ctccggaggg
     4201 cagcggcagc ggatcgcgat cgcccgtgca ctgctgcgca acacgcccgt gctgatcctc
     4261 gacgaggcga cctcggcgct cgacaccgag ctcgaacggc agatccagga gcagctggac
     4321 cagctcatgc acaatcggac gacgctggtg atcgcgcacc ggctgtcgac ggtggagaac
     4381 gccaaccgca tcctcgtcat ggacggcggt cgcgtcatcg aggaaggcac ccacgaagag
     4441 ctgctggccc gcgagggcca gtacgcagtg ctctaccgga tgcagttcaa cgaatgactg
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggcccgcgag ggccagtacg
     4621 cagtgctcta ccggatgcag ttcaacgaat gactgtcggc cccgggctgc agggactctg
     4681 gtacggtccg gcgtggcgga cggtcctgct gtggccaccg gcgctgctgt tcggtgtggc
     4741 cgtcgccgcg cgacgcacgc tgtacgccgc cggtgtgctt cgtcgatcgc gcgtggacgt
     4801 gccggtcgtc gtggtcggca atctcaccgt cggtggaacg ggcaagacac cggtcgcggc
     4861 ctggcttgca cgccagctca cgctgcgagg gcaccgcgtg ggcgtggttc tgcgcgggta
     4921 tggcggccgc gactcccgcc gtgtgcgcgt cgtcgagccg ggcgacagtc ctcgggacgt
     4981 cggtgacgag gcgctgctgc acgcagccgg tggtccgcac gtggtggtga tcggcgcgga
     5041 tcgcgtggct gcggcccgca tggcggcgga tcgcggcgct gcgatcgtgg tctgcgacga
     5101 tgggctgcag cacctgcgcc tcgcgcgcga ctacgagatc gctgtcgtgg acgcggaccg
     5161 tggattcggc aacggttgga tgctcccggc cggaccattg cgcgagccga ctggtcggct
     5221 cgaaagcgtc gacgcggtgg tgcggacgtg gcgcagcgag ggcagcgaca cgcccgtgca
     5281 tccgcggcgc ccgttcgtcc tcgacgcgcg gctgtcgatt ggcgctgcgg tgaacctcgc
     5341 gtcgggagag cgctgcgacc tggggcgctt ccggggcggg cgggtccacg cggtggccgg
     5401 catcggcaac ccgcaggcct ttttcgctgc gctggtggcg gccgggctcg acgtcgaagg
     5461 acacgcattg ccggaccacg ccgcaatcat accgacgcag atcgggctgg ccgctggtgc
     5521 gacggtcctg atgaccgcga aggatgccgt aaaatgccgg gagtgtgccg agcccggctg
     5581 gtggtacgtg gaactcgacg tttgcgtcga gcgtgatgcc gccggggaat tgctcgcgat
     5641 gatcctcgac cgcgccggat tgaccggcgc cggagcccaa attggatagc aagctgctcg
     5701 atatcctggc ctgcccggtc tgcaagggcc cgctgcaatg gcaccgggac gtgccgtgcc
     5761 tcgtctgccg cgccgaccgc ctggcgttcc cggtgcgtga cgggattccc gtgatgctgg
     5821 aagaggaggc gagacgactc gaccaggacg acgcgctgct caaggattga aggtggcatt
     5881 caaggtcgtc attccggccc gctacgcatc gacacgactg ccgggcaagc cgctcgccga
     5941 catcggcggc caggccatga tccagcgtgt agtgcagggc gcctgcgcaa gtggcgccgc
     6001 ccaggtggtc gtggcgacgg acgacgagcg cgtcgcctcc gcggtgcgcg atccggcggg
     6061 caagggccgg gtgaccgcgc tcatgaccga caccgcattg ccttccggga ccgaccgcgt
     6121 cgccgcagtg gccgagcgac tcgcgtgggg tgaggacacg atcgtcgtca acgtgcaggg
     6181 agacgaacca ttcctgccgg ccgcgttgat agaccaggtc gccggattgc tggcgaggga
     6241 tgcgaccgcc gggatcgcga cgctcgcgac gccggtccgg tcactcgagg agttcctgga
     6301 cccgaacgtg gtgaaagtcg tggcggccga cgacggcgcg gcgctctatt tcagccgcgc
     6361 gccggtgccc tggacacgcg atggggccat ggccggcctc ggcagccagc gcgagttcgg
     6421 gggcgcatcg cggcacatcg gcctctacgc gtatcgcgtc gcgacgctga agcgaatcac
     6481 ccggctgccg ccttcgaggc tggagtggct ggagaagctc gagcaactgc gcgcgttgca
     6541 gtcgggcgtg cgcatcgtcg tggcggaatg ctcagtggcg ccgggcccgg gcgtggatac
     6601 gcaggcggac ctcgagcggg cgcggcagcg agcggcgggg ctgaatggcg attccggtgg
     6661 aggcaagaca tgaaccaggt cctggtcacg gcgttcggca tcaccctcac gccctggaag
     6721 ctcgtcggct atctcggcgt ggcactgttc gcgggtcgct ggttcgtgca gatgtggtat
     6781 tcccggtcgc acggcaggcc ggtcgtgcca acggtgttct ggctcatgag catttccggc
     6841 agcctgctgc tgctcgcgta cttcactttc ggcaagaacg actcggtggg gatcctgtcg
     6901 aacctgttcc cggccggtgt cgccggctac aacctctacc tcgacctggc gcacaagcgc
     6961 tcgctgcgag agcctagcgg cgcaggctga gcgtccgggg cgagcgcacg aggccgcgca
     7021 gttcctcggg agtcaggttg cgccagcggc ctagagggag atcgccgagg cgaatgtgca
     7081 tgatgcgcac gcgctgcagg ctgcggacgg tgtagccgaa gtgctcgcac atgcgccgga
     7141 tctggcggtt gagtccctgg gtgagcacga tgcggaaggt gttgcgcccg acctgggtca
     7201 ccttgcacgg gttggtgacg gtgccgagga tgggcacgcc ggccgccatg ccggccagga
     7261 actccgcagt gagcggacgg tcgaccgcga cgacgtactc cttttcgtgc tggtgttcgg
     7321 cacgcagcac ggtgttgacg acgtcgccgt cgttggtgag caggatcagg ccctcggagt
     7381 ccttgtccag ccgcccgatc gggaaaacgc gttcgcggtg cgcgacgaaa tccacgatgt
     7441 tgtcgcggac ctcgcgatcg gtcgtgcact cgatgccgac cggcttgttg agtgcgaggt
     7501 acacgcgctt cggcctggcg cgcagcggct ggccgtccac cagcaccgcc tgtccttcgg
     7561 cgacctgcgt gcccagcacg gctttcactc cgtcgaccgt gacgcgcccg gcctcgatcc
     7621 actggtcggc ttctcgccgc gagcaggcgc cggtctcgct caggtacttg ttgatgcgca
     7681 tgcgcgctca cccgcccgca cgtttcaccg tgtagcccag tgtgccgagt tcggccacga
     7741 gcacgtcccg gtgatcgccc tggatctcga tcgctccgtc acggaccgtg ccgcccgagc
     7801 cgcagcgctt cttgaggcgt gtcgcaagct cgtccagggc atcgcccgag agcggcaccc
     7861 ccgtcacgac cgtaacgccc ttgcccttgc gacccttggt ctcgcggccg acgcggacga
     7921 tgccgtcgcc acgcggagcg gcggatccga ccgccggctt gctgcgccgg caccgacagt
     7981 ccgcgatggg tcgggcacac tcggggcata acttgccgcc gctggtggag tagacgagcc
     8041 tcgatgtgga catgctcctg gccctagcgc aggggcgcga cgcgatagat cgcgcccgcg
     8101 tagtcgtcgg cgacgtacac cgcgccgtcg gatccctcgg cgacaccggc cggccgaccg
     8161 atcacgccgg agctgctcag gaatcccgtg aggaagtcgc gctcctcgat cgagccgtcg
     8221 tcgcgccagt gcagcgacac caccttgtat ccgtccggga ccgaacgatt ccaggagccg
     8281 tgcagcgcga caatggccgc gcgctcgtac ccgggcggcc gtgacggcga acgcaggaag
     8341 acgatgccga gcggtgcgtt gtgcgcgcgg aagccgtgca ccggaggccg ggactgcgcg
     8401 agcagcgctt cgtggccgtg accgaggtcc gggtccagca cgttgtagcc gttgacgtac
     8461 ggccacccgt agaaccggcc acgctcgatc cggttcagtt cgtcgggcgg gaagtcgtcg
     8521 ccgagcaggt cccggccgtt ctcggtcgcg tacagcgcgc cgtcccacgg cgcccagtcg
     8581 aagccgacgc tgttgcgcag cccggtcgca tggatttcgg catgcgatcc gtcgggatcg
     8641 aatcgcatca tcgtcgcgcg acgatggtcc cgctcctcgc acacgttgca cgtcgagccc
     8701 tgcgagagat acagcttccc gtccggcccg aagccgatgg tgcgggtcac gtgattgccg
     8761 tcaccggtga gtccggtgat gacgtgggcg tagctgccgc tcgtcgtacc ggtcgcggcg
     8821 tcgaaacgaa tcctgcccac cgccgtcctc tccgcgacgt acagccaagc gtcgtggacc
     8881 gcgagtccat gggggtagtc gaggccgtcg agcaggatcc ggcggcggtc ggggcggccg
     8941 tcgccgttgg catcccgggc gaggatctgg acgttgccga cccggctctg cgccaccagc
     9001 agatcgcccg ccggcgtcat cgcgagccac cgggcagccg tcacgccacc ctcgtagatc
     9061 cggacctcga acccggacgc ggccaccagt tgctgcgcca cggtcgtctc actcggcgac
     9121 gcgatgccgc gtccgacgat catgtcgagc agcagcaggg gatcaccgac ccgccagggg
     9181 ccgaggcgca gcagcgcata gacagcagcg acggcagcca ctgccacgat gatcacggcg
     9241 gtcgccctgc gtttcatccc acctcggtgg cggcgcggaa ccacgtgccc atgctagcag
     9301 gcgcctgcgc aggaccgcag tggtacgttc gcagcgtcgg cacgccgggc gacggaccgg
     9361 cgcaatgcgt gactgcgata tgatcgaagc cgccgctgcc ggatggtggc ggccgtgatg
     9421 gatgggcgca atctcggccc ggaggaatgt ggagcatgcg cagggcagcg attgtcagcc
     9481 cgctcaggac gccggtcgga gcgttcggcg gcagcctgcg cggcgttccc gtcgagacac
     9541 ttggggcggc cgtcgtcaag gcgattctgg agcgaaccgg gctcgatccc gcacggatcg
     9601 aggacgtcgt gttcgcgcag tcctacgcga acggcgagac gccctgcacg ggccggtgga
     9661 tagcgctcga agccggactg cccatcgagg tgcccgggat gcaactcgac cgtcgctgcg
     9721 gcggcgggct gcaggcggtc gtgaccgccg cgatgatggt ccagtcgggc gcggcggacg
     9781 tggtgctggc gggcggcgtc gaaagcatga gcaacgtcga gtactacacg acggacatgc
     9841 gctggggcac gcgcgccgga tcggtgaagc tgcacgatcg cctcgaccgc ggccgggagc
     9901 gttcgcaacc ggaacatcgc ttcggacgca tctcaggaat gatcgagacc gccgagaacc
     9961 tcgccaggga gcacggaatc acgcgtgcag cggctgacga atacgcggcg ctgagccagc
    10021 agcgtgcggc ggctgcgtgg gcgggcgggc ggttcgatgc ggaggtcgtg ccgctgcacg
    10081 tgccgcagaa gaaaggcgac ccgctggtct tcgcccgcga cgagggcatc cgtgccgaga
    10141 ccacgccgga agcgttggca cggctgcggc cgatcatgcc gggcggtacc gtcactgcgg
    10201 ggaactcgag ccagcagaac gacgcggccg cggcctgcct cgtggtcgcg gagtcgctgc
    10261 tgccgacgct cggcctgcag cccgcgggat tcctggtcgg ctgggctgcc gcaggttgcc
    10321 atcccgcgac catgggcatc ggtcccgtgc cggcggtgcg cagggccttc gagcagacgg
    10381 gcctgtcgtt cgaccggatg gacctcgtcg agttgaacga ggccttcgcg tgccaggtcc
    10441 tggcggtgct caagtgctgg aactggcacg atcccgggcg gctgaacgtc aacgggtccg
    10501 gtatctcgct cggtcacccg atcggtgcga ccggcgcgcg catcctggtg acgctgctgc
    10561 acgaactcgg gcgccgcaag gggcgctatg gcctcgagac gatgtgcgtc ggcggcggcc
    10621 agggcatggc ggccatcttc gagcgcgcgt gaaagccggc aggcgcatgg aatcgaccgg
    10681 caagcatcgg gtcctcttcg tctgcatggg caacatctgt cgctcgccga cggcggaggg
    10741 cgtgttccgc cgcctcgtgc aggagcgcgc ggccgacctc gacatagaga tcgactcggc
    10801 aggaacgcac gactatcacg tgggtgaacc acccgaccgc cgcgcgatcg ccgcggcctt
    10861 gcgccgcgga atcgacatcc ggcagttgcg ggcgcgcacg gtcgaggccg cggacttcga
    10921 gcgtttcgac ctgctgatcg caatggaccg gctgaatcgc gaagtcctcc tcgaacgcag
    10981 cccgccgcaa tttcacgcgc ggatccggct gttcctggag ttcgctccgg acgcccacga
    11041 ggaggacgtc ccggacccgt attacggcgg agcagtgggg ttcgagcgcg tgctcgacct
    11101 cgccgaggag gcctccctgg gcctcctcga cgaggtgatg tcacgggccg gccggcggcg
    11161 ctgatcgccg ccgagcgtta cggctcgttg cgcggcgtct cacgcgtcga gtcgttctgc
    11221 ggcgcagggg tccattcctg gacgtcgtgc ggcggacgga cgacgggagg cgacggaggc
    11281 gaatagggca ctggctcagg gcggtgctcg aagtcgaatg agccagacct tggcgccgag
    11341 gtttccccag caggcggagc gtacgccggt gcgggtggtg caaccggcgc cgcgggtggc
    11401 gttgcaggcc gtgcgggagc ggcttccggg gcagcgaccg ggggcgtcca ccgctcacct
    11461 tcaggccgcg ctccttcgac acgtggggag tcgtcgaagg tcccgtcggc acccgcggca
    11521 tcgccgcgct gctcgggcgc gccgaatgcc tcgccgccac cctcgcgccg acgccgacga
    11581 ccgcctcgtc gcgagcgacg cccgctgcgc tcgccgcgtt cgccgtccgg gcgagcaccc
    11641 tgctcgccgc gctgtccctc gccgggcggc tggccttcac tgcgttgctg ggcttgcccc
    11701 tgatccggtc gcgactgctg cactggttcg ctgcgctgcg gctgggcctg cggctgtccc
    11761 tgtggctgtc cctggcgttg cgactggcca tcgggtcttc gatcacgctg tccgccgcct
    11821 tgcgactgcg gctggtccgg cctgcgtccg cgctcgtcac ggcgctgctc gccgcgcggc
    11881 gcgtcccgcc gctcgtcgcg acgagcggcg ggacgatcat tgcgatcgga gcgctgctca
    11941 ccgcgctgtt cgccacgcct tgcgtcgcgt ccacgctcgt cccgtccgcg ccgcgcgtcg
    12001 cgatcgtgtg agcgatgccg gtcgcgatcg cgctcgcggt cacgatcccg gtcccggtcc
    12061 cgatggtcgc gccggccgcg cgaggattcc gccacgggtt cgggcgcacg cttgtcgccg
    12121 aacagccagc gccacagccg gacgaacgct ccgtcgcgcg ggccaaactg cggcgccacc
    12181 gcgactgcgg gtgcaggggg ggcggcgacg gccacgggtt ccggtgccgg cggcggggcc
    12241 ggggtgctcg gcacgaccgt cgtcaccgcg ggcaccggcg gcgggggagg agccttctcc
    12301 tcggcggcga tcgcctcgag gtcgatcgtc ggctgcaccg ccatctggtg gctgatctgc
    12361 gagaactccg ggaggtcgac ctcgtcgtcg cgcacgcgac gcagttcgta cgccggggtc
    12421 tcgagatagc ggttcgggac caggatgacg tcgacctcgc tcttggtctc gatgccgtgc
    12481 agccaatcgc gcttctcgtt catgagatag gtcgcgacgt cgaccggcaa ctgcgcgatc
    12541 accttggcgg tgcggtcctt gcgcgcctcc tcgccgatca ggcgcaggat ggcgagcgcc
    12601 agcgactcga cgctgcggat gacgcccatg ccgttgcagc gcgggcagac gatgttggtc
    12661 gactcgctga tcgagggccg cagccgctgc cgcgacagct cgagcagccc gaagcgcgag
    12721 atccggccga tctggatgcg ggcgcggtcc tgcttcaccg cgtcgcgcag gcgatcctcg
    12781 acggcgcgct ggttcttctg cgactccatg tcgatgaagt cgatgacgat caggccgccc
    12841 acgtcgcgga ggcgcatctg gcgggcgatc tcgtccgccg cctcgaggtt ggtgttgagc
    12901 gccgtggtct cgatgtcggt gccgcgggtg ctgcgcgccg agttgatgtc gatcgacacc
    12961 agcgcctcgg tgtggtcgat gacgatcgag ccgccggacg gcaggttcac cttgtgcgaa
    13021 tacgcggatt cgatctggct ctcgatctgg tagcgcgtga acagcgggac gtggtcgtcg
    13081 tagaacttga gcttgcgcag gttgcccggc atgaagcgct gcatgtagtc ctgcgcctcg
    13141 gcgtagatct cctgcttgtc gaccaggatc tcgccgatgt cgtcggacag gtagtcgcgc
    13201 agcgcgcgca ggatcgcctt ggactcctgg tagatcagga acggcgccgg gcggccttcg
    13261 gaggcctgct tcaccgcgtt ccagatgtcc acgaggttgt tgacgtccca ctgcagttcc
    13321 tcggccgtgc ggccgacgcc ggcggtgcgc acgatcgcgc ccatgccgtc ggggatctgc
    13381 aggctgtcca tggcctcgcg gagctggtcg cggtcgtcgc cctcgatgcg gcgggagacg
    13441 cccccggccc gcgggttatt gggcatgagc accaggaagc gaccggccag gctcacgaag
    13501 gtcgtgagcg ccgcgccctt gttgccgcgc tcttccttct cgacctggac gatgagctcc
    13561 tggccttcct cgatcaggtc gcggattccg ggacggcctt cgccgccgcc gtcgcgggcc
    13621 ttgaagtagt cgcgggagat ttccttgagg ggcaggaagc cgtggcgctc ggcgccgtag
    13681 tcgacgaacg cggcttccag gctgggctcg acccgggtga tcttgccctt gtagacgttg
    13741 gccttcttgg tctcccggga cgggatttcg atgtcaaggt cgtagatccg ctggccatca
    13801 accagcgcta cgcgcaactc ctcttcctgg gttgcgttga cgagcattct cttcatcggt
    13861 cctcgctgaa tacgagctgg ggaccgtgaa cccccggacg ggcggtgcct tgggcctgtg
    13921 gagaggtctt gcagcggctg cagtcatgcg gctgttgttt tctcgcctgc gcgcattcga
    13981 gcgcggacgt tcgaccgaat cctggttatc tggcatcgat cccgccgcgg ctcgcgccgg
    14041 tccgccgccc gccggggctg cggcacctgc gcgtgttcac ggcagtgcga tggtctgacg
    14101 catcagaacc aactaagatg cgcgcgcctc gggcccgtgg gtccttggtc gcgacgcgga
    14161 ttgggcgccc ttccgaccgc acgaggcggt tcggcagcgc ttcgaccaag gactctagca
    14221 agcgatcgcc ggtaaatcaa tgggttacga caacaatgcg acggccccgg atcagcccgg
    14281 gcgcccgggc gtcggcgtcc gctacgtgga agccggcgaa ggggacgacg gccagcgcct
    14341 cgacaacttc ctggtccggg tcctgaaagg ggtaccgcgg actcacgtgt accggctgct
    14401 ccgcaagggc gaggtgcgcg tcaacagcaa gcgcgccaag ccggaccaga gggtggcggc
    14461 tggcgatcgc atccgcattc ctccggtgcg gcaggcggag acaccggcag ccgccgcgcg
    14521 ggctccgagc cgctccctgc aggacctcat cacccactcg atcgtgtacg aggacgacga
    14581 cctgctcgtg ctgaacaagc cggccggggt cgcggtccac ggcggcagcg gcatggccca
    14641 cggcgtcatc gaggtgctcc gggctgcgcg tcccgaagcc agggaactgg acctcgtgca
    14701 tcggctcgac cgtgacacca gcggatgcct gatcgtcgcg aagcggcgcg cggcgctgcg
    14761 ggacctgcac gcgcaactgc gcgagggaac gaccgagaag ctctacctcg cgctcgtgtg
    14821 cggcaagtgg aacctcggcc agaagcgcat cgagctgcct ctcgcgaccg gcgagcggcg
    14881 aggtggcgag cggcacgtcg ccgtgcgcgg gcacgggcag gtcgcggtga gcacgttccg
    14941 gccggtccag ttcttcggca acgtggcgac gctgatggag gtcgccatcg acaccggcag
    15001 gacgcaccag atccgcgtgc acgcggctta cgccgggcat ccggtcgcgg gcgacgacaa
    15061 gtacggagac cgcgagaaga atgcagcgct gcgtcagtac ggcgtcaacc gcatgttcct
    15121 gcacgctgcg tcgatcggcg tgaaccgtcc cggctcgcgc gagccgctgc acgtgagtgc
    15181 gccgctcggc gaggacctgc gagcggtcct cgacgcgctg gtcaaggcac cgggatcccg
    15241 gcgcggcgca gcgcagcgca gagccagatc aacggcaggc cggtgagtcc cgtcgggtcc
    15301 ccggactcga tgcgatcgaa cagcgagatg ccgagcgact cggccttgaa tccgcccgcg
    15361 cagtcgacgg ggttctcggt cgccacgtag cgacggatct cgccgtcacc cagcgcgcgg
    15421 aaccgcacca cggtgcggtc cacgtgcgac tccgcgctga ccgtcacgcc ggacacgacg
    15481 tgcaccgccg tcaggaacac gacttcgcgc ccggacgaac gatgcaactg ctcgatgcag
    15541 ttctcggtcg tgccgggctt gccgagcacg tcgtcgccga gcacagcgac ctggtcggag
    15601 ccgatgacca cggcacccgc tcgtgcggtt gcgaccgact cggccttgag gcgtgcgagt
    15661 cgttgcgcga gttcgagcgg cggctccgct ggctgccggg attcgtccac gtcgggcgcg
    15721 atgcactcga aatcgaggcg caggcgcgcg agcagcgcgc gccggtacgg cgacgtcgat
    15781 gccaggatca gccggggtgc aggggcgacg ctcacggccg gctccgggcc gcgctctggt
    15841 ccttgaaaaa caagggttta agctcgaatt cgttttgaca ctggaagggc gcgtcactat
    15901 tatacgcggc ccatgtcgcc agcccggtcg gatcaggtca acgcggtcga gctggctggc
    15961 cgggccgcca cgctggaacg cgagttcgac ctcacgcagt tgccgcggct ggtcgaggca
    16021 ggggcactcg ccgggacgcg agtgcacgcg gtgatggcct tcagcaagtt cgaaggccgc
    16081 ccgatggtga gcgtgcgtgt cgacggccgg gtgatgttgc cgtgccagcg ttgtctcgcg
    16141 ccgagcgagt gccgcatcga cgacgcgtcg cagctggtga tcgtcgcgga tgacacggcg
    16201 gacgtcccgg gtggattcga ggcagcgatc ggcgacgtcg agcacttgtc ggtcgcggag
    16261 ttggtcgagg aacaggtgct gctcggcatg ccgctggtgc cgttgcacga ggacgagtcg
    16321 cagtgcggcc cgagcgccgc gccgcaagcg gtcgtcgaac ccggttcggc ggcggaagag
    16381 atgcagagac ccttttcgaa cctgcgggaa ttgctcgacc agggcgagcg ctgaccgcga
    16441 caggagtttg agatcatggc agtccagaag agccgcaaga cgccctccaa gcgaggcatg
    16501 caccggtcgc acgacttcct cgtgaagccg gcgctctcgg tcgatcgcac cagcggcgag
    16561 acccacctgc gccaccgcgt cacgccggac ggtttctacc gcggcaagaa ggtgatcgag
    16621 aagccggcgg ccgaggaagg ccaggaataa tcccggccgc ccgcgcatga ccgccgggct
    16681 cgtcatcgcc gtcgacgcca tgagcggcga ccacggcgcc gaagtcacgg ttccggccgc
    16741 gctcgacgtg ctcaccgcga tcccggacct gcgcatcgcg ctggtcgggc gcggggacgt
    16801 cgtgcgcccg ctgtgcggca cgttcgtgcc cgagcgctgc tccatcgtcg aagcgagcga
    16861 agtcgtcggc atggacgagc ggccgcagga cgcgctccgc cgcaagaagg attcctcgat
    16921 gcgcgtcgcc atcaacctcg tcgagaaggg cgaggcgacg gcctgcgtgt cggccggaaa
    16981 cacgggtgcg ctgctcgcca cggcgcggtt cgtgctcggc atgatccccg gcatcgatcg
    17041 gcccgcgatc gtctcggcgg tgccggcggc gcacgggcac acggtcatgc tggacctcgg
    17101 ggcgaacccg gactgctcgg ccgatcacct cgtgcagttc gcggtcatgg gatcggtcat
    17161 cgccgccgac ctgcacggca cggagcggcc gcgagtcggg ttgctcaaca tcggcgagga
    17221 agacatcaag ggcaccgacg cgatccgcga tgcgcacaag cggctggccg cggcgccgat
    17281 caactacgtc ggtttcgtcg agggcacgga catcttctcg ggcgacgtgg acgtcgtcgt
    17341 gaccgacggg ttcaccggca acgtggcgct caagtcgatg gaaggcctcg cgcgattcat
    17401 cagcggcgtg atgcgtgagg agttcacgcg caccccggtg cgcaagatga gcgcgctggc
    17461 cgccacgccg gtgctgaatg ccctgaaggt gcgcctggac ccgggcgcgc acaacggcgc
    17521 gagcatggtt ggtctgtcgg gagtcgtgat caagagccac gggggcgcga accgtgcggc
    17581 attcgcgaac gcggtgcgcg ttgcgatcgc cgaggcgcgc aggggcgtgc cggcgcagat
    17641 cggcgaactg atgtcgcgtg tcgcggcgac cgcgcagccg gccgcgtcgc cgcacggttg
    17701 acccgtcgct cagcgttcgc cgctgcgacg ttcccgctcc cgccgccggt tcatccgggt
    17761 caccagttgc gcgcgccgcc gccgcagctt ctccgcgtcc gccttgcgtc gcgacaggga
    17821 ccgccggtaa cggacgaagt cctcgtaggc ctcgatctcg tgccgcaact cgcggaagac
    17881 cagttcgatc atcacggcgt cgtcgagcag gccgatgccg ggaatgctgt cgggtatgag
    17941 gtcgtccggg tcgcacaggt aggcgagggc ggccagcacg ggccctcgct ccgcatccgt
    18001 cagggtccag tcggcgtccg tgaccatggc gagcatcgcc tcgagcttgg acagccgccc
    18061 ggtgacgaag tccggcaggc tcgaaccgcg cagcgtggca atgagttccc tcgcggctgc
    18121 gacgatctcg tcgtcgtccg cgatgcgcac ggccgcgcgg gccttgcgca gttcgcggcg
    18181 gaagtggcga acgtcacgtg gcgacagttc gatggtgagc ctgtaggtca tcgacttccc
    18241 ccctgcgccg cgcaggctac tggccgggat accgtccggt caacgcacgg ccccgtgtgc
    18301 cgttagaatg cgctcagcca tgaaaaggtt gcacatgctc gtccgccccc ggtccgcctg
    18361 gatcggctgg ctcgctgcag cgtcggctgc gctggcctgc gccgacgctg gcgcggcgga
    18421 gtacgtgctg cccgcgaacg gcgacaacgt catcggccgc aattccacgg cgatcgcggc
    18481 ccgcgaggac accctcttcg acatcgcgcg ccgaacgggc gcgggttacg aggaaatggt
    18541 cgccgccaac ccgggcgtgg atccctggtt gccgggcgac ggcacggagg tcctgatccc
    18601 gggccgctac atccttcccg acgccccgcg caagggcatc gtcgtgaacc tcccggaaca
    18661 ccggctctat tactacccgc cgccgaagaa gggcgagccg gccgtcgtgc ggacctaccc
    18721 gatcagcacc ggcaagatgg actggaagac gccgatcggg gtcacgcaga tccagtcgaa
    18781 acaggagaag ccgaactggt acccgccggc ctcggtgctg aaggagcacg aggagcgcgg
    18841 cgacccgctg ccgaaggtcg tgccgcccgg cccggacaat ccgctcggcg aatacgcgat
    18901 gcgcctcgcg atccccggcg gtgcgtacct gatccacggc accaacaagc ccgcgggcgt
    18961 cgggatgcag gtcacgcacg gctgcatccg catgtacccg gaggacatcg cggagctgtt
    19021 cccgctggtg ccggtcagga cgcaggtcaa cctgatcgac cagaccaaca aggtcggctg
    19081 gcagcgcggc gtgctctacg tcgagcggca cgcgccgctc gaaggcaccg acaacccggc
    19141 ggacatggat ccgtccgtga tggaccagct cgtcagcgca gcggccgcag gccgccccgt
    19201 cgagatcgac tgggcgctgg tgcagcggac cttcgagcag gcgacgggag ttcccgtccc
    19261 cttgtcggtg ccggtgcgcg cagcggcgcg ctgaaatcgc agctgcggcg cgcagcaccg
    19321 cgacatcgta cgcagaaaag aaaacgccgc ggcattgccg cggcgtcttc gttcgtcttg
    19381 cgaagtatcg cggacgatta cttgcccatc gacttctgga acgcgcgatc gatcttctcg
    19441 ctgttcgcgt cgcagcaggc ctgcgcggcc ttcgcagcct ggccggcctg gttggcggtg
    19501 ttgttggcca tgttggcggc gtcggcggcg gccttggagg cgtcagcggc ggacttggca
    19561 gtcgcgctgg cggcagcggc ttcctgctgg gcagccttca cggccgcgtc attctgcgcg
    19621 acatattcat cgaacttgga ctggctcacg caaccagcga gggcgacggc ggcggccgcg
    19681 acggtcagga ccttggcgat ggcagttttc atgtctgtct ttccttcgtg tgaggggaat
    19741 ctcgggagcc aactacgccc gtccggatga tggcccggaa ccttggttcg cagtagagca
    19801 ggaggatgct aaccgatcgc tggcagatgt aaaaggcgag gaaaaagcga cgttgacgcg
    19861 ggggtgggca tggtctaggg gcgaaaatcc cacgcaacac ttgcgtcgtc gcccgctcct
    19921 gtatataaga acagtcactg tacatatata caggagccgc catggacgac ctcaccccgc
    19981 gccaacgcca ggtcctgcgc ttcatccagg acgccgtgtc cgagcacggc atgccgccca
    20041 cccgcgccga gatcgccgag gcgctcggct tcaagtcggc gaacgcggcc gaggagcacc
    20101 tgcgcgccat gcagcgcaag ggcgtgctcg agctcaggcc cggcgcctcg cgcggcatac
    20161 agctcaagga ctcgctgcgc gaccagatcg gcctgccgct catcggccgg gtcgccgccg
    20221 gccggcccat cctcgccgag gagaacatcg aggcgcacta ccagatcgat ccgcagctct
    20281 tccagccgcg gccgcattac ctgctcaagg tgcgcggcat gagcatgaag aacgccggca
    20341 tcctcgacgg cgacctcgtc gccgtgcacc gcacgcccga ggtgcgcaac cggcagatcg
    20401 tcgtcgcgcg cctcgagaac ga
//
LOCUS       DAOMFD010000017        20379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_475_length_20379_cov_35.481352, whole genome shotgun sequence.
ACCESSION   DAOMFD010000017 DAOMFD010000000
VERSION     DAOMFD010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20379
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_475_length_20379_cov_35.481352"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..731
                     /gene="glnL"
                     /locus_tag="PL152_02070"
     CDS             <1..731
                     /gene="glnL"
                     /locus_tag="PL152_02070"
                     /inference="COORDINATES: protein motif:HMM:NF008293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HQR48090.1"
                     /translation="HQRILRENALLTQLGGSRAMVRQLAHEIKNPLGGLRGAAQLLER
                     QLKDPALHEYTTVIISEADRLAALVDALLGPGQPPRKEPVNVHEVVQHVGHLLAAEAP
                     NGVAIERDYDPSLPRLRLDRNQVIQALLNLGRNAIQSVGERGRVVLRTRALTNASIGS
                     RRHRVVACIQVEDDGPGVPADLKDTVFYPLVTGRPGGTGLGLAVAQDLVGRHDGLIEF
                     ESRPGRTVFTILLPYDSNELGEST"
     gene            709..2106
                     /gene="ntrC"
                     /locus_tag="PL152_02075"
     CDS             709..2106
                     /gene="ntrC"
                     /locus_tag="PL152_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HQR48091.1"
                     /translation="MNSAKALDVWLVDDDASIRWVLERALRQGGMQTTSFEEAESVLA
                     ALKRAEPDVLVTDIRMPGRSGLDLLEEIRTQRPKLPVIVMTAHSDLDSAVAAYQGGAF
                     EYLPKPFDIDQALELVRRAAAQSHRSPASAVESRRIPEMLGHAPAMQEVFRAIGRLSR
                     SSMTVLITGESGTGKELVARALHRHSPRAAKPFIALNTSAFTADLLESELFGHEKGAF
                     TGAGELRRGRFEQADGGTLFLDEIGDMSPQLQTRLLRVLAEGEFYRVGGQLPVKVDVR
                     VIAATHQDLEERVKAGLFREDLLHRLNVIRIEVPPLRQRREDIPELLQHYLDVAAMEL
                     GVAPKTLTEEASATLAGFDWPGNVRQLVNACRRLTVLAAGREIALADIPADLGGAGAS
                     ASSLDWSNALASWARARLATGGAPLLEDAMPEFERTLIREALAAAHGGRQDAAKLLGW
                     GRNTLTRKLKELGME"
     gene            complement(2151..2963)
                     /gene="murI"
                     /locus_tag="PL152_02080"
     CDS             complement(2151..2963)
                     /gene="murI"
                     /locus_tag="PL152_02080"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HQR48092.1"
                     /translation="MPQDIDHPGCIGVYDSGVGGVSVLRALRVALPHESFVYVADSGY
                     APYGDQPTDFVEARARTVAGFLTDRGVKAMVLACNTVSVVAARHLRSQHPLPIIAMEP
                     AIKPAVTASRSGVVLVLGTTNTLRSESVGRLCERYASQARIVLSACPGLVECVERGEI
                     DTPGLRSHLAEYLSPGLEAGADTIVLGCTHYAFLAAVIADLAGPAVSIIEPSAAIARQ
                     LARRLGEMQEPAAKLAAQRTIFYTSGDPGALRSFLTAVREPCKAIRALPPLA"
     gene            complement(2989..3960)
                     /locus_tag="PL152_02085"
     CDS             complement(2989..3960)
                     /locus_tag="PL152_02085"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF005574.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HQR48093.1"
                     /translation="MTPVTLPPSLAVDYCGLRLASPIVLLSGCVGFGEEYTRVDGFSN
                     THVGAIVLKGTTGEPRLGNPPHRVYETPMGMLNAIGLQNPGVDHVVDRILPTLDFSET
                     RFIANVCGSTIDEYVRVTRRFDDSPIDAIEINISCPNIKEGGVQFGNVPEMSARVVAA
                     CRAVTRKPLITKLSPNQTDIRESARVCIEAGSDGLAVINTMMGMAIDVKTRRPVIGNV
                     QGGLSGPAIKPIALLKVMQVAEVARPLGIPIIGQGGITTAQDALEFLIAGATTVGVGT
                     ALFYDPLVCPKINTGVADYLAANGHASVRELVGSLDRPARKVEACTC"
     gene            4095..5282
                     /locus_tag="PL152_02090"
     CDS             4095..5282
                     /locus_tag="PL152_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carcinine hydrolase/isopenicillin-N
                     N-acyltransferase family protein"
                     /protein_id="HQR48094.1"
                     /translation="MCDTMVTVLPGRVLFAKNSDRDPNEAQVLEWHSRADHARGCALK
                     CTWIEIPQVPATHAVLLSRPYWMWGAEIGVNEHGVAIGNEAVFSRQPYAARGLTGMDL
                     VRLGLERGATAEEAVSVIAELLERHGQGGGCGHERRGFTYHNSFLVADPGGAFVLETA
                     GRRWQAQRVTGAWSISNGYTLPAMQAEADALRTRVTGCAMRRPRTLAAAERAAGPADL
                     MKVLRDHGDGPCPRYRLLNGAMAAPCMHAGGILAASQTTASWVSELRRDSSAHWVTGT
                     AAPCTALFKPVRVEEALDLGPPAVERADGRSLWWRHERFHRRVMRDPARLRPLFEAER
                     DATEARWIADPPDPAAAFAEGDRLLARWTARIGAEPAPDRRPWLVRRYWHSRNVRAAL
                     TGP"
     gene            5391..6764
                     /locus_tag="PL152_02095"
     CDS             5391..6764
                     /locus_tag="PL152_02095"
                     /inference="COORDINATES: protein motif:HMM:NF018716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1329 domain-containing protein"
                     /protein_id="HQR48095.1"
                     /translation="MEIRGGKGAVVPVVLAMCLMAGPVRAAVTAEQAAALGTTLTAFG
                     AEMSASADGEVPAYAGGIPALHAGTGTGDAATQDPFADERPLRVITPANADQAAAQLT
                     AGTQELLRRYPTFRVDVYPAHRSAAYPPWFLDNTRRNATAAVTTDGGLGIKNALPGVP
                     FPIPADGYEVMWNHRLRYMGRALELRFEAWIEDGAGQSVMTGAALGRWEFPVYDAAHR
                     DRTVKQDEVLFLWKSDYTGPQRRAGEAMLMVEVVNPLQQPRHVWSYVPGQRRTRLVNM
                     PDDAPSSSSSGAYIVDDAFVFNGTLDRYDVKLLGKREMLVPYNTYRFSFQANPARILQ
                     PHHIDPDLVRWELHRTWVVEATLKAGQHHPYGRRVFYVDEDTWTALASDEYGADGRLL
                     RAVFTFPTFDVETGVPFACTFVPYSFDTGGYYLGYHPESDTALRYVEPLPDSGWSPDA
                     LAGAGVR"
     gene            complement(6771..7199)
                     /locus_tag="PL152_02100"
     CDS             complement(6771..7199)
                     /locus_tag="PL152_02100"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQR48096.1"
                     /translation="MLRVSQLLSRKGRDVWSVDADEPVLEAIQIMADRRVGALPVTRG
                     RELVGLVSERDYARKVILLGRSSAETPVWEIMSSPVVTVPPTESVRRCMEIMTERRIR
                     HLPVLENGAMIGMISIGDLVRAVIEEQAQTIEHLEKFISG"
     gene            7313..>7550
                     /locus_tag="PL152_02105"
     CDS             7313..>7550
                     /locus_tag="PL152_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQR48097.1"
                     /translation="MFHVILHEPEIPPNTGNVIRLCANTGATLHLVRPLGFRLDAKAV
                     RRAGLDYRELAEVRIHSSLAACLESLAHPRWFAYT"
     assembly_gap    7551..7650
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7651..7929
                     /locus_tag="PL152_02110"
     CDS             <7651..7929
                     /locus_tag="PL152_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HQR48098.1"
                     /translation="SLAACLESLAHPRWFAYTTRAASTYEQAHYRPGDALVFGAETRG
                     LPADALAACPEECRLRIPMTAGARSMNLSNAVAVVVYEAWRQQAFRGS"
     gene            complement(7957..8967)
                     /locus_tag="PL152_02115"
     CDS             complement(7957..8967)
                     /locus_tag="PL152_02115"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HQR48099.1"
                     /translation="METGTKPVVAVLGSGSWGTALAVHLARTGHRTILWGIETDELVA
                     MTRDRVNNRYLPGVKLPDRVEIDHDFAHAVGEAEQLLVVVPSHAFREVLERARPLLRP
                     GQRIAWATKGFELSTGKLPHEVADQVLGTSVPTAVLSGPTFAREVGAGLPSAMTVASR
                     DGEYAMALARGISSESFRAYASGDMTGVEVGGAVKNVLAIAAGASDGLGYGANTRVAL
                     ITRGLAEMMRLGLALGAKRETFMGLAGLGDLVLTCTDDQSRNRRCGLLLAAGRTLDQA
                     QAEIGQVVEGVLAARAVHAVARRLGVVMPICEQVYLVLYEGRPPRDAVQALMQRTIKA
                     ES"
     gene            complement(9002..9481)
                     /gene="secB"
                     /locus_tag="PL152_02120"
     CDS             complement(9002..9481)
                     /gene="secB"
                     /locus_tag="PL152_02120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA];
                     GO_process: GO:0050821 - protein stabilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HQR48100.1"
                     /translation="MTEPNAQPQPAPDPHAPLVNPQAVYIKDCSFEAPAGPFMSGAEG
                     APTVNLNLATRAAALAQDLHEVVLQVNLEAKVGDKTVWLLELQQAGAFTIRNLSAADV
                     GQVLALAAPNYLLSFARAAVADLVSRGGFPPFLLPLVTFEGLHARAAQQQAASPAVN"
     gene            complement(9529..9798)
                     /gene="grxC"
                     /locus_tag="PL152_02125"
     CDS             complement(9529..9798)
                     /gene="grxC"
                     /locus_tag="PL152_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005005995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HQR48101.1"
                     /translation="MTGTPDIVVYSTAWCGYCDRAKALLERKGLVYREIRVDEDPAER
                     QAMLARSGGRRTVPQVFIGDRHVGGFDELYALDKAGQLDTLLGRA"
     gene            complement(9804..10241)
                     /locus_tag="PL152_02130"
     CDS             complement(9804..10241)
                     /locus_tag="PL152_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR48102.1"
                     /translation="MDRLLEYLTRHPILVGGTAALAIAVLVYELSRARGAGQAVGPLD
                     AVRLLNQGALMLDVRSKAEFDSGHVIDARHVPQEELASSAETLKKYREKVVIVCCESG
                     MRSSAATRVLAAQGFARIVNLRGGLAAWRSENLPLVKADAKGK"
     gene            10328..11539
                     /locus_tag="PL152_02135"
     CDS             10328..11539
                     /locus_tag="PL152_02135"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQR48103.1"
                     /translation="MIGQPLHPARPARMVRARRWALSGLVLAAGLLLGTPAVPAPSKQ
                     PTPAQKEAELRRVNSRIEKVQKAVNEDIEKRDKLSAQLRDAELGVQKARKTLDQIHAE
                     RLATEARLQDLEQEERGLEQDLAAERGALAGELRAAYVNGREEELKLLLNQEDPATFG
                     RMLSYYGYFGRARAERIAGIRDKLEHLALVREKIAAEHERLAALEADQQQQVAALRSA
                     QESRTRAVAAIEGQIRNQRSELGRLQSQAQALERLIDQLRKALEETPVAKQAPFEPLR
                     GKLPWPVQQGRVLARFGQPRAGGSMRWQGMLIGTDRGARVRAPYAGRVVYADWLPGMG
                     LMVVLDHGGGYMSLYGHNEELFRKVGDAVSAGDVIGSVGDTGGHNEPALYFEVRRGRQ
                     PVDPETWLQKR"
     gene            11739..12920
                     /locus_tag="PL152_02140"
     CDS             11739..12920
                     /locus_tag="PL152_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQR48104.1"
                     /translation="MYARGTFVDRPVAEAPKPAAVRPAHALLDEVAEHVRREYVDAVP
                     SGRLEKAAVEGLVASLDPHSEFLDAAAYDEMRVSTAGTYSGVGIEVAEADDGRVLVVA
                     PIEGSPAERAGVLSGDELLEVDGRKVVPGSLDETIEHMRGLAGSHVVVVVARKGESGP
                     LRFDLERSEVHVRTVRAQRLPGGYGYVRVNHFSDSTPGDFDGAIEEMQRAAGGSPLRG
                     LVLDLRDNPGGVLESAVSVADSFLDSGVIVRADGRTPDARFEMDATPGDVLEGAPVVV
                     LVDGGSASGAEIVAGALRDHGRATLMGERTYGKGSVQTVVPLRDGEALKLTTSRYFTP
                     SGRSIQQRGLEPDVKVASELSDKPRDFGRDAGSDPEVRLALQYLRDRSLDTHVALATG
                     R"
     gene            12945..13514
                     /locus_tag="PL152_02145"
     CDS             12945..13514
                     /locus_tag="PL152_02145"
                     /inference="COORDINATES: protein motif:HMM:TIGR01383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HQR48105.1"
                     /translation="MPRVLVPLAEGFEEIEAVTVIDLLRRAGIEVHTASLAQPRVTGS
                     HGITLEADGELAAVDAADYDMVVLPGGMPGAEHLRQDERVIALLKQLATSGRYTAAIC
                     AAPSVLAHAGLLDGRSATSFPGFLRPDSAAGIRLTDSPVVVDGKVVTSRGPGTAIEFS
                     LALIELLAGRDAAQAVGGRLQSATAARGR"
     gene            complement(13528..13911)
                     /locus_tag="PL152_02150"
     CDS             complement(13528..13911)
                     /locus_tag="PL152_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQR48106.1"
                     /translation="MELVAVVIALALIEFVVLGMLVGRARGKYGVKAPATAGNEMFER
                     WFRVHYNTMELLVVFVPATWLFGQYVSPQWAAGLGLVYVIGRIVYVRSYIADPARREF
                     GFGMSVLPILVMVIGALLGAGRALI"
     gene            14014..14388
                     /locus_tag="PL152_02155"
     CDS             14014..14388
                     /locus_tag="PL152_02155"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQR48107.1"
                     /translation="MIGFLLRAAITALGLWVASEVLDGLVFDSPSKLIVAAILLGVVN
                     AFVRPLAFILTLPLTVLTLGLFLLVLNAAMVGLVAWIVPGFEISGFWTAVGAALIVSL
                     VSWAASSAIGSNGKVEVFTVRK"
     gene            complement(14441..15337)
                     /locus_tag="PL152_02160"
     CDS             complement(14441..15337)
                     /locus_tag="PL152_02160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQR48108.1"
                     /translation="MTGAADNDPRLRLKIVAGYAAIYLIWGSTFLVTRLGVRSLPPML
                     FAGARFLVAGLLVAVAAVGLGERFPGTAREWRYMVLFSLLLVTAGNGLSTTALTRLPS
                     NEGALLSAGSALWIAWLGALGPRGHKLTVRGNIGLLLGLVGVALLVWPRSSTGSGHFG
                     WQSLVLCGSLAWAVGTVLYRNAALPIGPVAFNAVMMGLGGVWLLAAGAVTGELPEWHW
                     DAGGLAALTYLAVFGSAVAYTAYMWLLKHSPADRVGTFAYVNPAIAAVLGWVVLGEAL
                     EPTQVAGMLVVLLGVALVTLPK"
     gene            complement(15334..17259)
                     /locus_tag="PL152_02165"
     CDS             complement(15334..17259)
                     /locus_tag="PL152_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0035673 - oligopeptide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006857 - oligopeptide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide transporter, OPT family"
                     /protein_id="HQR48109.1"
                     /translation="MNHSAVAPQLTFRAIALAIILAMLLAAANTYLGLFAGLTIASAI
                     PAAVVSMAVLRLLGGGSILENNIVQTGASAGSSIASGVIFTVPALVILGHWTDFRYAW
                     VLAIAGLGGILGVFFSVPLRRSLIVEQGLKFPEGVAAAEVLKAGDNPSQGVKILGLSA
                     LGGGFAKLAAGSGLRLIPDTAAASGFFGRWLAYFGTNLSPALLGVGYIVGLNIGALAL
                     IGGMISWNVAIPIYAAYFLPDNAELAQRLAGASAEDAAYGIWSAQVRYLGVGAMLVGG
                     IWALISIRSSLASGIRSGLAATRAGAAKVVSHTEQDLPMQSILIGLVLFTVPLAILYQ
                     MIVQNIGISLLMTVIMIVAGFVFTSVSAYMAGLVGSSNNPVSGITICTILFASLLLLM
                     LMGKGAALGPVAAIMIGSVVCCAACIGGDNLQDLKCGNIVGATPWRQQVMLAIGAASC
                     ALVMAPVLNLLLHAYGIGERTALHPKALAAPQATLMASVAKGIFGGSLPWGMIGIGAL
                     IGAAIIVVDEVLKARKAPFHAPVLAVAVGIYLPLELSVPIFVGGLIAHFVERRLGIRD
                     EGPAAERAKQNGILFAAGLITGESLMGILIAVPIVVTSSAEVLSLPDSLQFGRLLGLG
                     IVGALALWLYRTGSRRV"
     gene            complement(17289..>18798)
                     /locus_tag="PL152_02170"
     CDS             complement(17289..>18798)
                     /locus_tag="PL152_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M2 family metallopeptidase"
                     /protein_id="HQR48110.1"
                     /translation="RTIELLKLGLPAPAPDDPQKRAELAAVLAAMESKYSTAKYCPKG
                     PDSCRDSTALMEVVAASRNYDELLDAWQGWHDAARPLRADYVKFVALANEGARELGYK
                     DLGAMWRSGYDMPADDFTKETARLWAQVQPFYRQLHCYARARLAKKYGEDKVPAGKPI
                     PAHLLGNMWAQQWGTIYDLLEPYPGASNLDVDAALKAQGYDAIRITRSAEDFYESIGF
                     PALPQTFWDRSMLVKPRDREVVCYASAWQIDGYEDVRIKMCIKPDLDSLRTIYHEMGH
                     VYYDLWYKDQPFMFQNAAHDGFHEAIGDTINLSMTPAYLTKIGLLPPSKPSREAEINS
                     QMQMALDKIAFLPFGKLIDEWRWKVFSGEVTPANYNAAWWALREKYQGVAPPVPRSEA
                     DFDPGAKYHVPGNTPYTRYFLSFILQFQFQKALCDAAGYKGPLAECSIYGSKEAGAKF
                     AAMLSKGASQPWQDTLYELTGTRQMDASAILEYFAPLRAWLAEQNKGQKCGW"
     assembly_gap    18799..18898
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(18968..20338)
                     /locus_tag="PL152_02175"
     CDS             complement(18968..20338)
                     /locus_tag="PL152_02175"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQR48111.1"
                     /translation="MTSSALPAVARETWLGHPKGLFLLFATEMWERVSYYGMRALLVL
                     SLVAGVQTANPGFGWSQSEALKLYGLFTGFVYFTPLVGGWLADNYLGQRKCIIIGGLV
                     MAAGQFTLAYAIPGNLQLFFAGLVLLVIGNGFFKPNISTMVGDLYAEGDARRDSAFTI
                     FYMGINSGALIAPFVCSTLGENPEYGWRYGYMAAGIGMILSVIIQLALANRYLGDIGK
                     TPSAQVARARAGGRKDPLTADERDRLRVIFMLFVFIVLFWAAFEQAGGLMNLYASDKT
                     DRMVGTFEVPAGWFQSVNPLFIVLLGPVFSAMWAWLATHGRNPPTPRKMIFGLILTGV
                     GFLFMVAAALESNTGIKASMWWLVLAYLFHTMGELCISPVGLSMVTKLAPLRLASLMM
                     GVWFLINFVANLLSGIIGSYAESLGELTIFGGLAVTLFVFAVVLWMVSGTLVRWMHGA
                     EDVKQA"
BASE COUNT         3244 a         6923 c         7058 g         2954 t
ORIGIN      
        1 aacaccagcg catcctgcgc gagaacgcgc tgctcacgca gctcggcggc agccgggcga
       61 tggtccgaca gctcgcacac gagatcaaga acccgctcgg cgggctgcgc ggggccgcgc
      121 aactgctcga gcgccagctc aaggacccgg cgctgcacga atacaccacg gtgatcatca
      181 gcgaggccga ccggctcgcg gccctcgtgg atgcgctgct tgggcccggt cagccaccgc
      241 gcaaggagcc ggtcaacgtg cacgaagtcg tgcagcacgt gggtcacctg ctcgcggcgg
      301 aggcgccgaa tggtgtcgcg atcgagcgcg actacgaccc gagcctgccg cggctgcgcc
      361 tcgaccgcaa ccaggtgatc caggcgctgc tcaacctggg ccgcaacgca atccagtccg
      421 tgggcgaacg gggccgtgtc gtgctgcgca cgcgcgcgct caccaacgcg agcatcggca
      481 gccgccgtca ccgcgtcgtc gcgtgcatcc aggtcgagga cgacggcccg ggcgtgccgg
      541 ccgatctcaa ggacaccgtc ttctacccgc tcgtcacggg gagacccggc ggcaccggtc
      601 tcgggctggc cgtcgcccag gacctcgtcg gccgccacga cgggctgatc gagttcgaga
      661 gccgccccgg acgcaccgtt ttcaccatcc tcctgcccta cgacagcaat gaactcggcg
      721 aaagcacttg acgtctggct cgtcgacgac gacgcctcga tccgctgggt gctcgaacgc
      781 gcgctcaggc agggcggcat gcagaccacc tcgttcgagg aggccgaatc ggtccttgcg
      841 gcgctgaagc gcgccgagcc ggacgtgctg gtcaccgaca tccgcatgcc gggtcgcagc
      901 gggctcgacc tgctcgagga gatacgcacc cagaggccga agctgccggt catcgtgatg
      961 accgcgcact ccgatctcga cagcgcggtc gcggcctacc agggcggcgc cttcgagtac
     1021 ctgcccaagc ccttcgacat cgaccaggcc ctggaactcg tgcgtcgcgc cgcggcgcag
     1081 agccatcgct cgccggcctc ggcggtcgag tcgcgacgca tccccgaaat gctcggtcat
     1141 gcgcccgcga tgcaggaagt gttccgcgcc atcggccggc tgtcacggtc gagcatgacg
     1201 gtgctgatca ccggggaatc cggcaccggc aaggaactcg tggcccgcgc gctgcaccgc
     1261 cacagcccgc gcgcggcgaa gcctttcatc gcgctgaaca cgtcggcctt cacggcggac
     1321 ctgctcgagt cggagctctt cggtcacgag aagggcgcgt tcaccggcgc cggcgagctc
     1381 aggcgcggcc gcttcgagca ggccgacggc ggcacgctgt tcctcgacga gatcggcgac
     1441 atgtcgccgc agctgcagac gcggctgctg cgcgtgctgg cggaaggcga gttctatcga
     1501 gtcggcggac agctgccggt caaggtggac gtgcgcgtga tcgccgcgac gcaccaggac
     1561 ctcgaggaac gcgtcaaggc cgggttgttc cgcgaggacc tgctgcaccg gctgaacgtg
     1621 atccgcatcg aggtaccgcc gctgcgccag cgccgcgagg acatcccgga actgctgcag
     1681 cactacctcg acgtcgccgc gatggaactc ggcgtcgccc cgaagacgct gaccgaggag
     1741 gcgagcgcga cgcttgccgg gttcgactgg cccggcaacg tgcgccagct cgtcaatgcc
     1801 tgtcggcggc tgaccgtgct cgcggccggc cgcgagatcg cgctcgccga catcccggcc
     1861 gacctgggcg gagccggcgc gtcggcgtcg tcgctcgact ggtcgaatgc gctggccagt
     1921 tgggcgcgcg cacggcttgc cacgggcggc gcgccgctgc tcgaggacgc gatgcccgag
     1981 ttcgagcgca cgctgatccg cgaggcgctg gcggccgcgc acggcgggcg tcaggacgcg
     2041 gcgaagctgc tcggctgggg ccgcaacacg ctgacgagga agctcaagga actcgggatg
     2101 gagtgagccg cccgagtgcg gcgcccgggc gaccggggac ggcgcgtcgc tcaagccagg
     2161 ggcggcagtg cgcgtattgc cttgcagggt tcacgcaccg cggtgaggaa cgatcgcagt
     2221 gcgcccgggt cgcccgacgt atagaagatc gtccgttgcg ccgcgagctt cgcggcgggc
     2281 tcctgcatct cgcccagacg gcgcgcgagt tgccgcgcga tcgctgccga tggctcgatg
     2341 atcgacaccg ccgggccggc gaggtcggcg attaccgcgg ccaggaaggc gtagtgcgta
     2401 caaccgagca caatggtgtc ggcgccggcc tcgaggcccg gactcaggta ctcggcgagg
     2461 tgcgagcgca gcccgggcgt gtcgatttcg ccgcgctcga cgcactccac cagcccaggg
     2521 cacgcgctca gcacgatgcg cgcctgactt gcgtaccgct cgcacagtcg cccgaccgac
     2581 tcgctgcgca gcgtgttcgt ggtgccgagc accaggacga caccggaacg cgaggcagtc
     2641 accgcgggct tgatggccgg ttccatcgcg atgatgggca gcggatgctg cgagcgcaga
     2701 tgacgtgccg cgacaacgct caccgtgttg caggcgagca ccatggcctt gacgcctcgg
     2761 tccgtcagga agccggcgac ggtgcgcgca cgcgcctcga cgaaatccgt gggctgatcg
     2821 ccgtagggcg cgtatccgct gtcggcgaca tagacgaacg actcgtgcgg cagcgccacc
     2881 cgcagtgcgc gcagcacgga tacgccgccg acgcccgagt cgtagacgcc gatgcagccc
     2941 ggatgatcga tgtcttgcgg cacgcgagcc ccggcggcgc gatcgtgatc agcaggtgca
     3001 ggcctcgact ttgcgcgccg gtcgatccag cgagcccacg agttcacgga ccgacgcatg
     3061 accgttcgcg gccaggtagt ccgctacccc cgtgttgatc ttcgggcaca cgagcgggtc
     3121 gtagaagagc gcggtgccca cgcccacggt cgttgcgccg gcgatcagga actcgagcgc
     3181 atcctgggcg gtggtgatgc cgccctgccc gatgatcggg atgccgagcg ggcgcgcgac
     3241 ctcggcgacc tgcatgacct tgagcagcgc gatcggcttg atggccgggc ccgagagccc
     3301 gccctgcacg ttgccgatga ccgggcggcg cgtcttcacg tcgatcgcca tccccatcat
     3361 ggtgttgatc accgccagcc cgtcgctgcc cgcctcgatg cagacgcgcg cgctctcccg
     3421 gatgtcggtc tggttcggcg acagcttggt gatcagcggc ttgcgcgtca ccgcgcggca
     3481 tgcggccacg acgcgcgcgg acatctcggg cacgttgccg aactgcacgc cgccttcctt
     3541 gatgttcggg caggagatgt tgatctcgat ggcgtcgatc ggcgaatcgt cgaaccgccg
     3601 cgtgacgcgc acgtattcgt cgatggtgga accgcacacg ttggcgatga agcgcgtctc
     3661 cgagaaatcg agcgtgggca ggatccggtc gaccacgtgg tccacgcccg gattctgcag
     3721 cccgatggca ttcagcatgc ccatcggcgt ctcgtacacg cggtgcggcg ggttgccgag
     3781 gcgcggctcc ccggtggtgc ccttcagcac gatcgccccg acgtgcgtgt tggagaaccc
     3841 gtcgacgcgc gtgtactcct cgccgaaccc gacacatccc gagagcagga cgatcggcga
     3901 cgcgaggcgc aggccgcagt aatcgacggc cagcgacggc ggcagggtga ccggggtcat
     3961 gcgtgcaatt ctaagcggaa acgggcggtg ccaccgcgat cgcgcgcagg cgcggcgggc
     4021 cggtgaccgc ctgcgcatcc acggcagtgc gtctggtcga ccgtcaccgg gccggctggc
     4081 atgatcgcag ggcaatgtgc gacacgatgg tcacggtgct gcccggtcgc gtgctgttcg
     4141 cgaaaaacag cgaccgcgat cccaacgagg cgcaagtcct cgagtggcac tcgcgcgcag
     4201 accatgcgcg tggatgcgcg ctcaagtgca cgtggatcga gattccgcag gtgccggcca
     4261 cgcacgcggt gctgctgagc cgcccctact ggatgtgggg agcggaaatc ggggtcaatg
     4321 agcatggagt agcgatcggc aacgaagccg tgttttcgcg gcagccctac gcggcccggg
     4381 gcctcacggg catggatctc gtgcgcctgg gcctcgagcg cggcgccacg gcggaggagg
     4441 cagtgtcggt aatcgcggaa ttgctcgagc gccatggcca gggtggcggc tgcggccacg
     4501 agcgccgtgg cttcacttac cacaacagct tcctggtcgc ggacccgggc ggcgcattcg
     4561 tactggaaac ggcgggacgc cgctggcagg cccagcgtgt cacgggcgcg tggtcgatct
     4621 cgaatggcta tacgctgcct gcgatgcagg ccgaagcgga cgcgctgcgg acgcgcgtca
     4681 ccggttgcgc catgcggcga ccacgcacgc tcgcggctgc cgagcgcgct gcaggccccg
     4741 cggacctgat gaaggtgctg cgggaccacg gcgacggacc ctgcccccgt taccggctgc
     4801 tgaacggggc gatggccgcg ccgtgcatgc atgccggcgg gatcctggcg gcgtcccaga
     4861 cgaccgcctc gtgggtgtcg gaactgcgac gcgattcgag tgcgcactgg gtcaccggca
     4921 cggcggcgcc ctgcaccgcg ctgttcaagc ccgtgcgggt cgaggaagca ctcgatctag
     4981 gtccgcccgc cgttgagcgc gcggacggtc gcagcctctg gtggcgccac gagcgatttc
     5041 accggcgcgt gatgcgagac cctgcgcggc tgcgcccgct cttcgaagcc gagcgcgacg
     5101 cgaccgaggc gcgctggatc gccgacccgc cggacccggc cgcggcattc gcggaaggcg
     5161 accgcctgct cgcccggtgg acggcacgca tcggtgcgga gcccgcgccc gaccggcgcc
     5221 cgtggctggt caggcggtac tggcattcac ggaacgtgcg ggcggcgctg accggtccct
     5281 gaccggggag cgtggtgtcg taattctccg gattgtcacg cgtgaaggga gggacgagcc
     5341 tgccgtgact tcactcgaca ggcgcgagtc gtgcgcgcag ggggcggtcc atggaaatcc
     5401 gcggcgggaa gggggcggtt gtcccggtcg tcctcgcaat gtgcctcatg gccggacccg
     5461 tgcgggccgc cgtcacggcg gagcaggcgg ccgcacttgg caccacgctc acggctttcg
     5521 gcgccgagat gtccgcgagt gccgacggcg aggtcccggc atacgcgggc gggatcccgg
     5581 cactgcatgc cggcactggc acgggtgacg cggcgacgca ggatccgttc gccgacgaac
     5641 gaccgctgcg ggtcatcacc ccggccaacg cagaccaggc ggccgcgcag ctgacggccg
     5701 gcacgcagga gctgctgcgt cgctacccga cgtttcgcgt ggacgtctat cccgcgcacc
     5761 gctccgcggc ctatccgccg tggttcctcg acaacacccg ccgcaacgcg acggctgccg
     5821 tcacgaccga cggcggcctc gggatcaaga acgcgctgcc cggcgtgccc ttcccgatcc
     5881 cggcggacgg ctacgaggtc atgtggaatc accggctgcg atacatggga cgcgcactcg
     5941 aactcaggtt cgaggcgtgg atcgaggacg gcgccgggca gtccgtcatg acgggcgcgg
     6001 cgctcgggcg ttgggaattc ccggtgtacg acgccgcgca ccgcgacagg accgtgaaac
     6061 aggacgaggt gctgttcctg tggaagagtg actacaccgg cccgcagcgg cgcgcgggtg
     6121 aagcgatgct gatggtggag gtcgtcaatc cgctgcagca gccgcggcac gtctggtcct
     6181 acgtcccggg gcagcgtcgg acacgcctgg tcaacatgcc ggacgacgcg ccgtcgagca
     6241 gcagcagcgg cgcctacatc gtggacgacg cgttcgtgtt caacggcacg ctggaccgct
     6301 acgacgtgaa gctgctcggc aagcgcgaga tgctggtgcc gtacaacacc taccgcttct
     6361 ccttccaggc gaatccagcg cgcatcctgc aacctcacca catcgatccc gatctcgtgc
     6421 gctgggaact gcaccgcacc tgggtcgtgg aagcgacctt gaaggcgggc cagcaccatc
     6481 cgtacgggcg ccgggtcttc tacgtggacg aggacacctg gactgcgctc gcgtccgacg
     6541 aatacggcgc cgacggcagg ctgctgcgcg ccgtgttcac gtttcccacg ttcgacgtcg
     6601 agaccggcgt gcccttcgcc tgcacgttcg tgccctacag cttcgacacc ggcggctact
     6661 acctcggtta ccacccggag tcggacacgg cgctgcgcta cgtcgagccg ctgccggact
     6721 cgggctggtc gcccgacgcg ctcgccgggg ccggagtccg ctgaagcacg tcagccggag
     6781 atgaacttct cgaggtgctc gatcgtctgc gcctgctcct cgatcaccgc gcgcacgagg
     6841 tcgccgatcg agatcatgcc gatcatggcg ccgttctcga ggaccggcag gtggcggatg
     6901 cgccgctccg tcatgatctc catgcagcgt cgtacgctct ccgtcggcgg tacggtgacg
     6961 accggcgagg acatgatctc ccacacagga gtctcggcgg acgagcgacc aagcaggatg
     7021 accttgcgcg cgtaatcgcg ctccgacacg aggccgacca gttcgcggcc gcgcgtgacc
     7081 ggcaacgcgc cgacgcggcg gtccgccatg atctggatgg cttcgagcac gggctcgtcc
     7141 gcgtccacgg accagacgtc tcgacccttg cgggatagca gttggctgac gcgcagcatg
     7201 atcgtgtccc ccggggtggc gcgcaggcgg ctcgctccga ttctaaaccc gccgtgggca
     7261 ggaccgctgc aaccggttgc accgacggcg aggggacgcc acgatcggtg cgatgttcca
     7321 cgtcattctt cacgagcccg agattccgcc caacaccggc aacgtgatcc ggctgtgcgc
     7381 caacacgggt gccacgctgc acctggtccg gccgctggga ttccggctcg atgcgaaggc
     7441 ggtacgacgc gccggcctcg actatcgcga gctggccgag gtgcggatcc actcgagcct
     7501 cgcagcgtgc ctcgagtcgc tggctcaccc gcggtggttc gcctacacga nnnnnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn agcctcgcag cgtgcctcga gtcgctggct
     7681 cacccgcggt ggttcgccta cacgacgcgg gcggcatcga cctacgagca ggcgcactac
     7741 cgcccgggcg acgcgctggt gttcggtgcg gagacgcgtg gactgccggc ggacgcgctt
     7801 gcggcctgcc cggaggagtg tcgcctgagg atcccgatga cggcgggggc gcgcagcatg
     7861 aacctgtcga atgcggtggc cgtcgtggtg tacgaggcgt ggcggcagca ggcattccgg
     7921 ggttcctgac ccggcagcac cagcttgcgc gtccggtcag gattccgcct tgatggtccg
     7981 ttgcatcagc gcctgcaccg cgtcgcgcgg cggcctgccc tcgtagagca cgaggtagac
     8041 ctgttcgcag atcggcatga ccacgccgag gcgccgtgca accgcgtgaa ccgcgcgtgc
     8101 cgccagcacg ccttcgacca cctggccgat ctcggcctgg gcctggtcga gcgtgcggcc
     8161 ggccgcgagc agcagcccgc agcgccggtt gcgcgactgg tcgtcggtgc aggtgagcac
     8221 gaggtctccg aggcccgcga gccccatgaa agtctcgcgc ttcgcgccga gcgccagccc
     8281 aagtcgcatc atctccgcca ggccgcgcgt gatgagcgcg acgcgcgtgt tcgcgccgta
     8341 accgaggccg tcggaggcac ccgcggcgat cgccagtaca ttcttgaccg cgccgccgac
     8401 ttcgacgccc gtcatgtcgc ccgatgcata ggcgcggaac gactcggacg agatgccgcg
     8461 cgccagcgcc atcgcgtatt cgccgtcgcg cgacgccacg gtcatcgcac tcggcaggcc
     8521 ggcgcccacc tccctggcga acgtcggccc ggagagcacc gccgtcggca cggacgtgcc
     8581 gagcacctgg tcggccacct cgtggggcag cttgccggtc gagagctcga agcctttcgt
     8641 ggcccaggcg atgcgttgac ccggccgcag cagcgggcgt gcccgttcga gaacttcgcg
     8701 gaacgcgtgg ctcggcacca cgaccagcaa ctgctcggcc tcgcccaccg cgtgcgcgaa
     8761 gtcgtggtcg atttccacgc ggtccggcag cttcacgccc ggaaggtagc gattgttgac
     8821 ccggtcgcgg gtcatcgcca cgagttcgtc cgtctcgatg ccccagagga tcgtgcggtg
     8881 tccggtccgc gcgaggtgca cggcgagtgc cgtgccccac gagcccgaac cgagcacggc
     8941 cacgaccggc ttcgttcccg tctccacgac tggtcgcggg gtcgccacgt gggcgactac
     9001 ctcagttgac cgccgggctg gccgcctgct gctgggccgc ccgggcatgc aggccctcga
     9061 acgtcaccag cggcagcagg aacggcggga aaccaccccg gctgacgagg tcggcgaccg
     9121 cggcccgggc gaaggagagc aggtaattcg gcgccgcgag ggccagcacc tgaccgacat
     9181 cggcggccga gaggttgcgg atcgtgaacg caccggcctg ctgcaactcg aggagccaca
     9241 cggtcttgtc gccgaccttg gcctcgaggt tcacctgcag cacgacttcg tgcaggtcct
     9301 gcgcgagggc cgccgcgcgg gtcgcgaggt tgaggttgac cgtcggtgcg ccttcggcgc
     9361 ccgacatgaa cggccccgcg ggagcctcga aggagcagtc cttgatgtac acggcctgcg
     9421 ggttcacgag cggcgcgtgc ggatccgggg caggctgggg ctgggcgttc ggctcggtca
     9481 tggtgtggtc ctgtagtaga aaccggttgg atcggtggtt gccgctcgtc aggcgcggcc
     9541 gagcagcgtg tcgagctggc cggccttgtc gagggcgtag agttcgtcga acccgccgac
     9601 gtggcggtcg ccgatgaaga cctggggaac ggttcggcgt ccgccactgc gcgcgagcat
     9661 ggcctggcgc tcggccgggt cctcgtccac gcggatctcg cgatagacga ggcccttgcg
     9721 ctcgagcagc gccttcgccc ggtcgcaata gccgcaccag gccgtcgaat acaccacgat
     9781 gtcgggcgtc ccggtcacgg cggtcacttg cccttcgcgt ccgccttcac gagcggaaga
     9841 ttctcggagc gccacgcggc gagcccgccg cgcaggttca cgatcctggc aaagccctgt
     9901 gccgcgagca cgcgggtggc ggccgacgag cgcatgccgc tctcgcagca gacgatgacc
     9961 accttctcgc ggtacttctt cagcgtctcc gcgctggagg cgagctcctc ctgcggcacg
    10021 tgccttgcgt cgatcacgtg gcccgagtcg aactcggcct tcgaccgcac gtcgagcatc
    10081 agtgcgccct ggttcagcag gcgcacggcg tcgagcggac ccacggcctg gcctgccccg
    10141 cgtgcccggc tgagctcgta caccagcacc gcaatggcga gtgccgccgt tcccccgacg
    10201 aggatcgggt gccgggtcag gtattcgagg agtcggtcca tgggccgggg gtctgaaaaa
    10261 gggtcgcgga ttatagcagc cccgctagac ttgccggctt gaccccgccc cgaagcgcga
    10321 acgtggaatg atcggccagc cgctgcaccc tgcacgcccc gcccggatgg tccgcgcgcg
    10381 ccggtgggca ctgtcggggc tcgtgctggc ggccgggctc ctgctcggca caccggccgt
    10441 gcccgccccc agcaagcagc cgacgccggc gcagaaggaa gcggagctgc gccgggtcaa
    10501 ttcccgcatc gagaaagtcc agaaggccgt caacgaggac atcgagaagc gcgacaagtt
    10561 gagcgcgcag ctgcgcgacg ccgaactcgg cgtgcagaag gcccgcaaga cgctcgacca
    10621 gatccatgcg gagcgcctgg cgaccgaggc caggttgcag gacctcgagc aggaagagcg
    10681 ggggctcgaa caggacctgg cggccgagcg cggggcgttg gccggcgaac tgcgcgccgc
    10741 gtacgtcaac ggccgcgagg aagagctcaa gctgctgctg aaccaggaag accccgccac
    10801 gttcggccgc atgctcagct actacggcta tttcgggcgc gcgcgcgccg agcgtatcgc
    10861 cgggatccgc gacaagctcg agcacctggc gctggtgcgc gagaagatcg ctgcggaaca
    10921 tgagcgcctc gcggcactcg aagccgacca gcagcagcag gtggctgcac ttcgctccgc
    10981 gcaggagagc aggacccggg cggttgcggc catcgaaggc cagattcgca accagcgcag
    11041 cgagctcggt cgcctgcagt cgcaggcaca ggcactcgag cgattgatcg accagttgcg
    11101 caaggccctc gaggagacac cggtcgccaa gcaggcgccc ttcgagccgt tgcgtggcaa
    11161 gctgccgtgg ccggtccagc agggccgggt gctcgcgcgg ttcgggcagc cgcgggccgg
    11221 cgggtcgatg cgctggcagg gcatgctcat cggtaccgat cgcggcgccc gggtgcgcgc
    11281 gccctatgcc ggccgcgtcg tgtatgccga ctggctgcct ggcatgggac tcatggtcgt
    11341 cctcgatcac ggcggcggat acatgagcct ctacggtcac aacgaggaac tgttccgcaa
    11401 ggtcggcgac gccgtgtctg cgggcgacgt gatcgggtcg gtcggggaca cgggcggaca
    11461 caacgagccg gcgctgtact tcgaggtccg ccgcggtcgg cagccggtcg atcccgagac
    11521 ctggctgcag aagcgctgag gcccggcgcc ggcggggcga cctgggcgcg aatgtgaccc
    11581 gggccgcgac cagcggaggt cgctggccac cggaacgtgc ctgccgcggc ggtcgaggct
    11641 aaactgcgtg cccgttctac gatcgcgcgt ccggcttgaa tacacgcatc cacaccttgc
    11701 ttgccacact tgccggcgcc gtcgcagggg ctggtctcat gtacgcgcgg ggtacgttcg
    11761 tcgaccggcc cgtcgccgag gcgccgaagc cggccgccgt gcggccggcg cacgccctgc
    11821 tcgacgaggt ggcggagcac gtgcgccgtg agtacgtgga tgcggtgccg agcgggcgcc
    11881 tggagaaggc cgcggtggag ggactggttg ccagcctgga cccgcattcc gagttcctcg
    11941 acgctgcggc ctacgacgaa atgcgggtga gcaccgcggg cacgtactcg ggcgtcggca
    12001 tcgaggtggc cgaggcggac gacgggcgcg tcctggtcgt cgcgccgatc gagggctcgc
    12061 ccgccgagcg cgcaggcgtc ctgagcggag acgagctgct ggaagtggac ggccgcaagg
    12121 tcgtgccggg cagcctcgac gagaccatcg agcacatgcg cggtctcgca gggtcacacg
    12181 tcgtggtcgt ggtcgcccgc aagggcgaga gcgggccgct gcggttcgat ctcgagcgca
    12241 gcgaggtcca cgtgcgtacc gtccgcgccc aacgcctgcc cggcggctac ggatacgtgc
    12301 gcgtgaacca cttcagcgac tcgacgcccg gcgatttcga cggcgccatc gaggagatgc
    12361 agcgggccgc gggcggatcg ccgctgcgcg gtctcgtgct cgacctgcgc gacaaccccg
    12421 gtggcgtgct cgagtcggcc gtgagcgtgg ccgacagctt cctcgactcc ggggtgatcg
    12481 tgcgtgcgga cggccgaacc cccgacgccc gcttcgagat ggacgcgact ccgggcgacg
    12541 tgctcgaagg tgccccggtc gtcgtgctgg tcgacggcgg ctctgcttcc ggggcggaga
    12601 tcgtcgcggg tgcgctccgc gaccacggtc gcgccacgct catgggcgag cgcacctacg
    12661 gcaagggctc ggtgcagacg gtcgtgccac tgcgcgacgg cgaagcgctc aagctcacga
    12721 cctctcggta cttcacgccc tcgggtcgct cgatccagca gcgcggcctc gagcccgacg
    12781 tcaaggttgc gagcgagctg tcggacaagc cgcgcgactt cgggcgcgat gccggcagcg
    12841 acccggaagt gaggctggcg ctgcagtacc ttcgcgaccg cagcctggat acgcacgtcg
    12901 cactcgcgac cggccgctga cgagcgtccc aggagcacac gaccgtgccg cgcgtgctcg
    12961 tcccgctggc cgaaggcttc gaggagatcg aggcggtcac ggtgatcgac ctgttgcggc
    13021 gcgccggcat cgaagtccac accgcgtcac tggcgcagcc gcgcgtcacc ggctcccacg
    13081 gcatcacgct cgaagccgac ggcgaactgg ctgcggtcga cgccgcggat tacgacatgg
    13141 tcgtgctgcc gggcggaatg ccgggcgcgg agcacctgag gcaggacgag cgcgtgatcg
    13201 cgctgctcaa gcagctagca acgagcgggc gctacacggc cgcgatctgc gcggcgccgt
    13261 ccgtgctggc gcacgcgggc ctgctcgacg ggcgcagcgc cacgagtttt cctggcttcc
    13321 tgcggccgga ctcggcagcc ggcatccgac ttacggattc gccggtggtc gtggacggga
    13381 aggtcgtcac ttcgcgtggt ccgggcaccg cgatcgagtt cagcctagcg ttgatcgaac
    13441 tgctcgccgg ccgtgatgcg gcgcaggcgg tcgggggcag gctgcaaagt gcgacagcgg
    13501 ctcgaggccg ctgaagcgac gctggcctca gatcaacgca cggcccgcgc ccagcagcgc
    13561 accgattacc atcaccagga tcggcagcac gctcatgccg aatccgaact cgcgcctggc
    13621 cggatcggcg atgtagctgc gcacgtacac gatcctgccg atcacgtaga cgaggccgag
    13681 accggccgcc cattgcggac tcacgtactg gccgaacagc cacgtcgccg ggacgaacac
    13741 gaccagcagt tccatcgtgt tgtagtggac acggaaccag cgctcgaaca tttcattgcc
    13801 cgcggtggcc ggcgccttga ccccgtactt gccgcgcgcc cgccccacga gcatgcccag
    13861 cacgacgaat tcgatgagcg cgagcgcgat gacgacggcg acgagttcca tgcctgcctc
    13921 cccgggggcc attgattggt gtggggtgag gattccatta ccatgcccgc gaggtcaacg
    13981 tccgccgggc gaccatctca aggtgagact gccatgatcg gattcctgct gcgcgctgcg
    14041 atcacggcgc tggggctgtg ggtcgccagc gaagtcctcg acggcctcgt cttcgattcg
    14101 ccgtcgaaac tgatcgtcgc cgcgatcctg ctcggggtcg tcaacgcgtt cgtgcgtccg
    14161 ctggcattca ttctgacgct gccgctcacg gtgctgacgc tcgggctgtt cctgctggtg
    14221 ctgaacgccg cgatggtcgg gctggtcgcc tggatcgtcc ccggcttcga gatctcggga
    14281 ttctggaccg ccgtcggcgc cgcgctgatc gtgagcctcg tgtcctgggc cgcgtcgagc
    14341 gcgatcggct cgaatggcaa ggtcgaggtc ttcaccgtcc gaaaataggg gacattctcc
    14401 tattttccgc agcggaaaat aggagaatgt cccctgtttc ctatttcggt agggtcacca
    14461 gtgcgacgcc gagcagcacg acgagcatcc ccgcgacctg cgtcggctcg agcgcctcac
    14521 cgagcacgac ccagccgagc accgccgcga tcgccggatt gacgtacgcg aaggttccga
    14581 cgcggtcggc gggcgaatgc ttgagcagcc acatgtacgc ggtgtaggcg accgccgatc
    14641 caaacaccgc gagataggtc agcgccgcaa ggcctccggc atcccagtgc cactcgggta
    14701 gctcgccggt gaccgcaccc gccgccagca gccagacccc accgaggccc atcatgacgg
    14761 cattgaatgc gacgggcccg atcggcagcg ccgcattgcg atagagaaca gtgcccaccg
    14821 cccaggccag gctcccgcag agaaccagcg actgccagcc gaagtgccct gaccccgtcg
    14881 aactgcgtgg ccacacgagc aacgcgacac cgacgagtcc cagcagcaga ccgatgttgc
    14941 cgcgcaccgt cagcttgtgg cccctcggtc caagcgcccc gagccacgcg atccagagcg
    15001 cggatcccgc cgacagcaac gctccctcgt tggaggggag gcgcgtcagc gcggtggtgc
    15061 tgaggccatt gcccgccgtg acgagcagca gcgagaacag caccatgtag cgccactcgc
    15121 gcgcggtccc cgggaatcgc tcgccgagcc cgaccgccgc gacggcgacc agcagcccgg
    15181 cgacgaggaa acgcgcgccg gcgaacagca tcggcggcag cgagcgaacg cccaggcgcg
    15241 tcacgaggaa tgtcgagccc cagatgaggt agatcgccgc gtagcccgcg acgatcttga
    15301 gccgcagtcg cggatcgttg tccgcggcgc cggtcacacg cggcgcgacc ccgtccgata
    15361 cagccacagg gccagcgcgc cgacgatgcc caatccgagc aggcggccga actgcagcga
    15421 gtcgggcagc gagagcacct cggcgctgct ggtgaccacg atcgggacgg cgatcaggat
    15481 acccatcagt gactcgccgg tgatcagccc tgccgcgaac aggatgccat tctgcttggc
    15541 acgttccgcc gcggggcctt cgtcgcggat cccgagccgc cgctcgacga agtgtgcgat
    15601 caggccgccg acgaagatcg gcaccgacag ctcgagcggc aggtagatgc cgaccgcgac
    15661 ggccagcacc ggcgcgtgga acggcgcctt gcgcgccttg agcacctcgt cgacgacgat
    15721 gatcgcggcg ccgatcagtg cgccgatccc gatcatgccc cacggcaggc tgccgccgaa
    15781 gatgcccttg gcgacggagg ccatcagcgt ggcctgcggc gcggcgagcg ccttgggatg
    15841 cagggccgtg cgctcgccga tgccgtaggc atgcagcagc aggttgagca cgggcgccat
    15901 gacgagcgcg cagctcgcgg ccccgatcgc cagcatcacc tgttgtcgcc acggcgtggc
    15961 gccgacgatg ttgccgcact tcaagtcctg aaggttgtcg ccgccgatgc acgcggcgca
    16021 gcacacgacc gaaccgatca tgatcgccgc gaccgggccg agcgccgcac ccttgcccat
    16081 cagcatcagc agcagcagcg acgcgaacag gatcgtgcag atggtgatgc cggacaccgg
    16141 gttgttcgac gagccgacga gaccggccat gtaggccgac acggaggtga acacgaagcc
    16201 ggcgacgatc atgatgaccg tcatgagcag gctgatgccg atgttctgca cgatcatctg
    16261 gtagaggatc gcgagcggca cggtgaacag caccagcccg atcaggatcg actgcatcgg
    16321 caggtcctgc tcggtgtgcg acacgacttt cgccgcgccc gcgcgggtcg ccgccagccc
    16381 gctgcggatg cccgatgcca gcgagctgcg gatcgagatc agggcccaga tcccgccgac
    16441 cagcatcgca ccgacgccga ggtagcggac ctgcgcggac cagatgccgt aggcggcgtc
    16501 ctcggccgag gctcccgcga gccgctgcgc gagctcggcg ttgtcgggca ggaagtacgc
    16561 ggcgtagatc gggatggcga cgttccagga aatcatcccg ccgatcagcg ccagcgcgcc
    16621 gatgttcagc ccgacgatgt agccgacgcc gagcagcgcc ggcgagaggt tggtcccgaa
    16681 atacgcgagc cagcggccga agaacccgct cgccgcggcg gtgtccggga tcaggcgcag
    16741 gccgctgccg gccgcgagct tggcgaagcc gccccccagg gcggagaggc cgaggatctt
    16801 cacgccctgt gacgggttgt cgccggcctt gagcacctcg gccgccgcca cgccctccgg
    16861 gaacttgagc ccctgctcga cgatcagcga gcggcgcagc ggcaccgaga agaacacgcc
    16921 gaggatgccg cccaggcccg cgatcgccag gacccacgca tagcggaagt cggtccagtg
    16981 accgaggatg accagcgccg gcaccgtgaa gatcactccg gaggcgatcg acgaaccggc
    17041 cgaagcgccg gtctgcacga tgttgttctc gaggatcgag ccgccgccga ggaggcgcag
    17101 gaccgccatc gagaccaccg cggccgggat cgccgaggcg atggtgagcc cggcaaacag
    17161 cccgaggtag gtgttcgccg ccgcgagcag catcgcgagg atgatcgcga gcgcgatcgc
    17221 acgaaaggtg agctgcggcg caacggcgct gtgattcatg ttctcccccg ggttgctgcg
    17281 tcagcggctc accagccgca cttctgtccc ttgttctgct cggcgagcca cgcgcgcagc
    17341 ggcgcgaagt actcgaggat cgccgaagca tccatctgcc gcgtgccggt gagctcgtag
    17401 agcgtgtcct gccacggctg gctcgcgccc ttggagagca tcgccgcgaa cttcgccccg
    17461 gcctccttgc tcccgtagat cgagcactcc gcgagcgggc ccttgtagcc ggccgcatcg
    17521 cacagggcct tctggaactg gaactgcagg atgaacgaca ggaagtagcg cgtgtacggc
    17581 gtgttgcccg ggacgtggta cttggcgccc gggtcgaagt ccgcctcgct gcgcggcacc
    17641 ggcggcgcga cgccctggta cttctcgcgc agcgcccacc acgcggcgtt gtagttcgcc
    17701 ggcgtcactt cgccggagaa caccttccag cgccactcgt cgatcagctt gccgaacggc
    17761 aggaacgcga tcttgtcgag cgccatctgc atctgcgaat tgatctcggc ctccctgctc
    17821 ggcttcgaag gcggcaggag tccgatcttc gtgagatagg cgggcgtcat cgagagattg
    17881 atcgtgtcgc cgatggcctc gtggaatccg tcgtgcgccg cgttctggaa catgaacggc
    17941 tggtccttgt accagaggtc gtaatagacg tggcccatct cgtggtagat cgtgcgcagg
    18001 ctgtcgaggt cgggcttgat gcacatcttg atgcgcacgt cctcgtaacc gtcgatctgc
    18061 cacgcgctcg cgtagcagac cacctcgcgg tcgcgcggct tcacgagcat ggagcgatcc
    18121 cagaacgtct gcgggagcgc gggaaagccg atcgactcgt agaagtcctc ggcggaccgg
    18181 gtgatgcgga tggcgtcgta tccctgcgcc ttgagggcgg cgtcgacgtc gagattgctc
    18241 gcgcccgggt acggctcgag caggtcgtag atcgtgcccc actgctgcgc ccacatgttg
    18301 ccgagcaggt gggccgggat gggcttgccg gccgggacct tgtcctcgcc gtacttcttc
    18361 gcgagtcgcg cacgcgcata gcagtgcagc tgccggtaga aaggttgcac ctgcgcccag
    18421 agccgggccg tttccttcgt gaagtcgtcg gcgggcatgt cgtagccgga ccgccacatg
    18481 gcgccgaggt ccttgtagcc gagctcgcgc gcgccctcgt ttgcgagcgc gacgaacttc
    18541 acgtagtcgg cgcgcagcgg gcgggccgcg tcgtgccatc cctgccaggc gtcgagcagc
    18601 tcgtcgtagt tgcggctcgc ggcgaccacc tccatgagcg ccgtcgagtc gcgacacgaa
    18661 tccggcccct tcgggcagta cttcgccgtg ctgtacttcg actccatggc ggccagcacg
    18721 gccgcgagct cggcacgctt ctgcgggtcg tccggcgcag gcgcgggcag gccgagcttc
    18781 agcagctcga tcgtgcgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    18901 tacggagcat ggtgacctcg catgcaagtg tggaatcgcg acctcgccga cgcggcggcc
    18961 ggcagggtca ggcctgcttc acgtcctcgg cgccgtgcat ccatcgcacc agcgtgccgg
    19021 agaccatcca gagcacgacg gcgaacacga acagcgtgac ggcgagaccg ccgaagatcg
    19081 tgagctcgcc gaggctctcg gcgtacgaac cgatgatgcc gctcaacagg ttcgccacga
    19141 aattgatgag gaaccacacg cccatcatca gcgacgcgag gcgcagcggc gcgagcttgg
    19201 tgaccatgga cagcccgacc ggcgagatgc acagctcgcc catggtgtgg aagaggtacg
    19261 cgagcaccag ccaccacatg ctggccttga tgccggtgtt cgactcgagg gccgcggcga
    19321 ccatgaacag gaagccgacg ccggtgagga tgaggccgaa gatcatcttg cgcggggtcg
    19381 gcgggttgcg cccgtgggtg gcgagccacg cccacatcgc cgagaagacc ggcccgagca
    19441 gcacgatgaa cagcgggttc accgactgga accagccggc cggcacctcg aacgtgccca
    19501 ccatgcggtc cgtcttgtcc gaggcgtaga ggttcatcag gccgccggcc tgctcgaatg
    19561 cagcccagaa cagaacgatg aagacgaaca gcatgaagat cacgcgcagc cgatcgcgct
    19621 cgtcggcggt gagcggatcc ttgcggccgc ccgcgcgcgc gcgggcgacc tgcgcggagg
    19681 gcgtcttgcc gatgtcgccg aggtagcggt tcgccagcgc gagctggatg atcaccgaga
    19741 ggatcatgcc gatgcccgcg gccatgtagc catacctcca gccgtactcg gggttctcgc
    19801 cgagcgtcga gcagacgaac ggcgcgatga gcgctcccga gttgatgccc atgtagaaga
    19861 tggtgaaggc gctgtcgcgt cgcgcgtcgc cctctgcgta caggtcgccc accatggtcg
    19921 agatgttggg cttgaagaac ccgttgccga tcacgagcag caccagcccg gcgaagaaca
    19981 gctgcaggtt gccggggatg gcgtaggcga gcgtgaactg ccccgctgcc atgaccaggc
    20041 cgccgatgat gatgcacttg cgctggccga ggtaattgtc ggccagccag ccgccgacca
    20101 gcggggtgaa gtacacgaat ccggtgaaca ggccgtacag cttgagcgcc tccgactggc
    20161 tccacccgaa gcccgggttg gccgtctgca cgcccgccac gagcgacagc acgagcagcg
    20221 cccgcatgcc gtagtagctc acgcgctccc acatctcggt cgcgaagagc aggaagagtc
    20281 ccttggggtg gccgagccac gtctcccgcg ccacggcggg cagggcggaa ctggtcatgg
    20341 tttttgtttc tgcttcaatt gtttgcgcgg gatacgcgc
//
LOCUS       DAOMFD010000018        20342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_479_length_20342_cov_33.621432, whole genome shotgun sequence.
ACCESSION   DAOMFD010000018 DAOMFD010000000
VERSION     DAOMFD010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 20342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_20342_cov_33.621432"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="PL152_02180"
     CDS             complement(<1..704)
                     /locus_tag="PL152_02180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48112.1"
                     /translation="MVVDCASWNRWARPAVVLLMLVTGGCGGGGVGTGGSQGPDPIVL
                     DFPIAYVKRPVPTGPVMQTDARELLDFAPGGDLWVRDRASPSASERNVTAAITQGRGD
                     VRDVEPSYDGTKLVFALHRPLIDGATPDEQPTWEIWEYTVASQDLHRVIASDLVAHEG
                     NDVAPAYLPDGRIVFSSTRQRQSKAILLDEGKPQFDAQDEDRREPAFVLHVMNADGSD
                     IHQVSFNQSHDMDPAVL"
     gene            916..2106
                     /locus_tag="PL152_02185"
     CDS             916..2106
                     /locus_tag="PL152_02185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48113.1"
                     /translation="MRIGMPASPCATRLRAAATALCVVLATLASPALHADARTQAKRL
                     HDRLAGVPPTETVLSSMAADIAAGRPTDAAYIAMDNRAFYDVTLKNFAMPWTNRDQSV
                     FAPLNDYVATVIGMVRDDVPFDGVLYDDILYVGSGVAGVPAYSPASNDHYAALESQGA
                     NLKDSLVRTTQSAASGLPPEGTAGVLTTRAAAQAFFIAGTNRAMFRFTLINHLCTDLE
                     QVMDTSRPPDRIRQDVSRSPGGDSRLFLNNCIGCHSGMDPMVQAFAYHDWDETAGRIV
                     YTPGIVRPKYFHNKDTFPPGYSTPDDHWDNYWRAGRNSLMGWDASLPGSGQGAKSLGL
                     ELSHTDAFAGCQVKKVFRTVCLRDPSDAQDRAKVQAMVSSFKAGGYRMKQVFAEAATY
                     CMGD"
     gene            2118..4571
                     /locus_tag="PL152_02190"
     CDS             2118..4571
                     /locus_tag="PL152_02190"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LamG domain-containing protein"
                     /protein_id="HQR48114.1"
                     /translation="MNTTMRAWTLRAAALAAVLLLAGCGGGGAKTVENPVTSGGTPAA
                     YSGPPPATADIQAFKINVWDNLRATNRCGSCHVAGGQAPQFVRSDDINLAYAAANTVV
                     NLASPRDSRMVQKVAGGHNCWLASPAACADILTTWISNWAGTAAGGGTSGITLTAPPL
                     RDPGASKAFPASPALFSTTVYPVVRQYCSRCHAPAAASPQAPYFASADLNEAYAAVKV
                     KIDLDRPEQSRLVVRLRSEFHNCWSNCAADADEMEAAIRAFADQVPATQVDPSLVLSK
                     ALGMYDGIVASGGNRYTANLVGLWEFKTGKGTVAYDTSGVDPALNLTLSGDVTWVGGW
                     GINVKSGKAQGSTTASRKLTTLISATGEYSIEAWVAPGNVAQEDARIVSYSGGTTTRN
                     FMLGQTLYSYDFYNRSTSTGPNGDPKLTTAAADEDLQATLQHVVVTYDPVNGRRVYVN
                     GQSTGDVDPVSAGTIANWDDTFAFVLGNEVSGDRQFQGVFRLVAVYNRALTQEQVRTN
                     YAAGVGEKYYVLFSVSHLVNVPQSYIMFEVSQYDSYSYLFNKPTFISLDPAARPGTIP
                     IKGMRIGENGTEPDVGQAYRTLDTSITDSNYGPLGQPLSSIGTVIPLQKGPDSDEFFL
                     TFDVLGSHSNVRLDPVPLAPPPLPDVRRPADIGLRAFDRINATMSVLTGVSTQAPGVK
                     STFDTVRQSLPAVASVDTFASAHPVAIAQMSIQYCNSLVDDTTARAQYFPGFNFNAAP
                     ATAFDPTGRAIVLDSLVTHMMRANLATEPDPTAVRGDLDALITRLTACGSSCPAGRTP
                     TVVKATCAALLGSAVTLIE"
     gene            4603..6237
                     /locus_tag="PL152_02195"
     CDS             4603..6237
                     /locus_tag="PL152_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48115.1"
                     /translation="MIIRRKIPRRLALDEPIRHELAHKRPLTRRDFIAQGFTAGLGTV
                     VAPTIFSLFANPRAAMAALSPDLEALKASCGIQVQGAGKIPFICIDLAGGANMVGSNV
                     LIGGPGGQLDFLSTDGYAKLGLPGDMLPSMTNTATGTSFTDTRLGLAFHSDSAFLRGI
                     MDRVAPATAASINGAVIAARSENDTGNNPHNPMYGIYRAGANGSLATLIGSSPSDSGG
                     NSLAPAGMIDLSVRPTKVDRASDVTGLVDTGQLASLLSVSDTVAVMEAVQRISARKMA
                     VMDPKQPWADPVKTTVTRDEVVRELVSCNYLKAADVVDRFGDPSSLNPDLDPDIVGAG
                     GIFSPAEYNGDSEFRKTAAVMKMVLSGYAGAGTITMGGYDYHGQGRSTGELRDFRAGR
                     CMGACLEYAARRGMPLMLYVFSDGSISASGQVDDTIDGRGKFMWTSDNQSTAASFFLV
                     YNPQGRPSLLSSGSGGTARHQQIGYFRPDASVETASSPAANNVNLLVETVVLNYMALH
                     GEQGQFSQRFPTNGLGSASLQDSLIAFQPIVSGTIG"
     gene            6248..7621
                     /locus_tag="PL152_02200"
     CDS             6248..7621
                     /locus_tag="PL152_02200"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HQR48116.1"
                     /translation="MDMAVGVDHWTLLACPRCGGHLAPGDEPACSACGARYPVVDGLP
                     WLCADPAAALGGWRTRARSLVSALEQQSARYASAISPGETRAAARRRLELLANACNDH
                     ARRLKSLLAPLELAAGAAAPETYAALGAPLPAGQGLTSYYANLHRDWCWGAAENEAGY
                     SAVDRALGPGNPGRTLVLGCGGGRLAYDLHVRRRPAVTVAADLNPLMLAVASRMFAGA
                     QVELHEFPLAPRDLASHAVLRELRAPAPAPPGLWPIVADASHAPFAQAAFDTIVTPWL
                     VDILDEDFAVVAARVNQWLRPGGRWVNSGSWFFQQPDPARCYSIDELPELLHAAGFSD
                     VSVHEEVVPYLASPASSQARMERLVTWSAIAQAHVPAPPPHRNSPAWLERADLPLPLL
                     PQLESHVLALRVHAYVASLVDGRRTLAEIAAILVNDRLMRADEALPAVRAFLQRVYSD
                     AQPAVRP"
     gene            7618..8550
                     /locus_tag="PL152_02205"
     CDS             7618..8550
                     /locus_tag="PL152_02205"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HQR48117.1"
                     /translation="MTESAGGGWHEREAAIMGTRIAVQLWAEDPELARLGIDSVIAEM
                     HRVDRLMSTYKPESQLSQVNAHAHERPITVDPDIVDVVAKSFEYSRLSDGLFDVTYAS
                     VGHLYDYRAGRHPSDGEIAAALPAVDYRQVEVDRERCTIRFLKPGMRIDLGGFAKGWA
                     VDRGAAILRSLGIRNAMVTAGGDTRFVGDRLGKPWIVGIRDPRREGAVVTRLPLVDEA
                     VSTSGDYERYFEQDGVRYHHILVPGTGRSPAAVRSVTIIGPTATHTDGLTKPVFILGV
                     ERGMEYLRRVGDVDAVIIDAEGAVYYSPGLEPRG"
     regulatory      8656..8739
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            8740..9288
                     /locus_tag="PL152_02210"
     CDS             8740..9288
                     /locus_tag="PL152_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR48118.1"
                     /translation="MRGHDHYARARTEALIHTLAIAAAVIAGALAFPLAARSSTSPVT
                     VGAAAPDFTLKALDGRNLRLSEYRGDVVVLTFWASWCGACRPALEAANSFAAAQGKDG
                     AVVLGVSLDGDAARARSVAESLALKYPTLVDARQAVGRLYDIDALPFTLVVDRDGIVR
                     GEWSDGVAPQADLARTVAGVGQ"
     gene            9285..9851
                     /locus_tag="PL152_02215"
     CDS             9285..9851
                     /locus_tag="PL152_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48119.1"
                     /translation="MNRRYNAAARRRMIEWAGMLLVAGGVLCAGRAGAEDPAQETRAV
                     DQDVQDLKKQLVDLNRDLFKLEEEILYPASTQVAVFLAVNTGTFFQLDSVTLKIDDKE
                     VANYLYTEREAAALRRGGVQKLYLGNLKSGSHELVALFVGKGPHDVDYRRGATLTFEK
                     TVGAKYVELRISDREASMQPEFVVRQWD"
     gene            9961..11781
                     /locus_tag="PL152_02220"
     CDS             9961..11781
                     /locus_tag="PL152_02220"
                     /inference="COORDINATES: protein motif:HMM:NF019341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48120.1"
                     /translation="MTRALAAGPEAGDAPATKIRDLDYGDVLFHFYQDDYFGSLVRLE
                     AASDLSRMPHHDAEARLLAGGLSLSLGMYSEATRIFDSLLNASVPQSVSDRARFYLAR
                     IGYERGSPEEAWRNLGQVRGTLPAPLESERRVLAANVLMALGRYGEAAALLRKDTTED
                     DWTPYARYNLGVALVRSGDPEQGQQWLAAVGTMPAQTDEQRSLRDRANLALGYSLLQQ
                     RAADPAVAALSRVRLDGPYTNRALLGLGWAEADAKRPDRALVPWLELRQGRLLDSAVQ
                     ESLLAVPYAYAQLASNGQAAQQYRYAVQSYAEEAKRIDQSIAAIQDGAFLDSVLDKAP
                     EGDFAGWTWQLQRIPDAPQTRYLYHLLASHEFQDGLRNYRDLRVMQRNLAGWRQTLEA
                     FDDMVATREQAAREREPLRESTMAAADIDGLAARQAMLQQRVADIEGSRDVVALATVD
                     EAREWHTLQQAQARIAALPPGPQRDALVQRANLLQGVLFWQMDAAYKVRLRAAKVELR
                     EIDSLLASARDGQQRVGAASAAAPRDTAGFADRIQALAARVNAMGPRIDAAAVAQQRV
                     LVAIATDELEAQKRRLASYATQAQFALAAIYDGATAGVVR"
     gene            11778..14978
                     /locus_tag="PL152_02225"
     CDS             11778..14978
                     /locus_tag="PL152_02225"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR48121.1"
                     /translation="MSARIVIAIACVCASVPAAARTRSAEPATLGDLQSRSAPVSRDV
                     AVEADPGLATRSYEDFLRIPSTDAGLRAQALRRLGDLRLAESEALRARDGTESPAAQE
                     ADRQAIAAYRQLLDEQPDAPGTDAVLYQLARAYEGLGESAAAFTTLDQLVTRYPQSPH
                     YDEAQFRRGETFFAEQRYADADRAYAAVQALGPSSEFYEQAVYKRGWALFKQSRDTES
                     SAEFLVLLDHVLVRDGRLRPAADISRADQELTSDALRALAITFAANDAIETLREVLDR
                     HGPAPYESRLYASLGDLYVEKERYQDAAEAYRAYATRQPDDPEAALMIVRATDAYSRG
                     GFPSLVLDGKRQLVEQYGPQSDYWRRHAPDVDPRVSDAVQACLLDLARHHHALAQKGS
                     AADRDLAIRWYRDYLAGFDSSPQAPATRLLLADLLFEGGRYEEAAGEYERVAYSYASS
                     PDAAHAGYAALVAWQRAAEQAPASARAAIDAKGIDSSLRFAGTFPDDPEAVAVLTRTA
                     KALFDAGDRKRAEPVAQQVLAYGARADGAQQLVAWTVLAHTWFDDGRYADAERAYREL
                     ASRLPAGDPQMAEVTERLAASVYRQAEARQAAGDTQGAVQEFLRVVAVAPASSVSAKA
                     QYDAATLLLDSKQWSAAADVLQSFRAAHPDDALAPEATRKLAVAYMEAGRGRDAAVEF
                     ERVADDGKEDQAVRRAAQWQAAELYAAAGDVPAASRAYANYVERFPSPADTAIEARQE
                     LAALASKSGDAQAREHWLKEIVAADQAAGSARTDRTRLLAAQASLELARPLDQTARGI
                     ALVVPLDRALARKKSAMESALGAYSHAADYGIVEVTTAADYAMADLYRDLAKALLASE
                     RPRGLNAEELEQYDLLLEEQAYPFEEKAIAILESNARRAATGTWNEWVRKSYAALAEM
                     KPARYARTERLDAPDAAPGTAPDVALALTAARTAIEAGDSAAAIEDADAALKLDPANA
                     VAHNMLGVACRNLGRFPEARTAYESAIAQAPGHATPQRNLGVLLDLYLGDPAAALPHY
                     ERYQELTGGSDPETGPWLVEIRTRLGQVQRTAEANP"
     gene            14975..15415
                     /locus_tag="PL152_02230"
     CDS             14975..15415
                     /locus_tag="PL152_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48122.1"
                     /translation="MKRMTMLVVALALAAPAVVRANEPASTPASTPARSASPAKAAKP
                     ATPATVAPATQVAATTGNRAHDRLTLDSTAITGNRELPKVMSIVPWKEAEAPQGPDRP
                     MGSLIEEVLSPIDRAEFQREVVYYRDLTAPKSVTQSPTPGATKP"
     gene            15412..16137
                     /locus_tag="PL152_02235"
     CDS             15412..16137
                     /locus_tag="PL152_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR48123.1"
                     /translation="MNAIDLVANFFKQGGVFLYPIAAVFAVGVAVAIERFIYLGKVQR
                     GNRRLWAELAPLIQAGNFRKAMEVASRTDSALATIMRYGLTRISTARRRDDIEKAMEE
                     SLIEVIPRLEKRTHYLATLANIGMLMGLLGTVVGLINAFAAVASVNPSEKAALLAASI
                     SVAMNNTALGLGLAITLLLSHMYLETRTTELVDSLEVASIKFLNSVTERRDPAADKLD
                     ESDASAVVARARARDALAGAVRA"
     gene            16134..16643
                     /locus_tag="PL152_02240"
     CDS             16134..16643
                     /locus_tag="PL152_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR48124.1"
                     /translation="MKSSALARRQQRNHARYRGRNDLNIVPMLDVMTILAFFLIFTAV
                     FSKTSVLELDLPGPGARAAATAPPIELEVIVTRARIDIADRSVGVLTSVPATANGQDV
                     ARLSVLLGQIKERYPAVDSATLLVAEDVDYDTIVQVMDAVRVRQETDGPRVTRTELFP
                     HISMGDAPT"
     gene            16640..17146
                     /locus_tag="PL152_02245"
     CDS             16640..17146
                     /locus_tag="PL152_02245"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR48125.1"
                     /translation="MIRTGLKRHARHGRAGGGHGPLALVPMIDMLTILVVYLLVHAAD
                     TEILPNTRNISIPQSISEQKPREATVVTVTRDMLYVNGEAIVSVADVGRSAEPVVEPL
                     RAALARQPANLLGGKDGQHEVTVMAEKSLPYAVLRKVVASCSAADYSKVSLAVVEREQ
                     ALVASIGA"
     gene            17159..>17867
                     /locus_tag="PL152_02250"
     CDS             17159..>17867
                     /locus_tag="PL152_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48126.1"
                     /translation="MSTAIAVNFRIYDLPWTPSAVDERRLRRIVGAALGLLIAFGIVI
                     PLLPERPRDHTVPPAIPDRIVEFLIERPKPPPKPEPKIEPPPTPVTAPAIEHPAPVKA
                     PVVQSEPKPDPRKKAASAGLLALADQLQELRDLSVEDRPDARPLNAGSGDKARVDRAL
                     LTARTGEGSGGITVGQASHGFGGGATGLKGISTAQVSAGAEASADPGVQRAGKSSKAA
                     RTREEIELVFDRNKSAIY"
     assembly_gap    17868..17967
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17968..18252
                     /locus_tag="PL152_02255"
     CDS             <17968..18252
                     /locus_tag="PL152_02255"
                     /inference="COORDINATES: protein motif:HMM:NF033768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgmX/PglI C-terminal domain-containing protein"
                     /protein_id="HQR48127.1"
                     /translation="RTREEIELVFDRNKSAIYAIYSRALRDNPALQGKVVLEVTIAPS
                     GEVTDCRIVSSDLGDADLEHKLIARVKMFRFEAKDVAPMTTTKPIEFFPA"
     gene            18338..20173
                     /locus_tag="PL152_02260"
     CDS             18338..20173
                     /locus_tag="PL152_02260"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009337 - sulfite reductase complex
                     (NADPH) [Evidence IEA];
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HQR48128.1"
                     /translation="MSTSSTALPLSPLDPERNALLHRVVDGLDPASLHWLSGFAAGVA
                     HARAGGGAAEVLAPPALPGIPVAARSEPVARLAVVYGSQSGNSKRVADRLGRVAEEAG
                     LAVRVYAARDYPLKDLAKERYLSVVISTQGDGDPPDDARSFVEHVTGKRAPRLENLAY
                     SVLALGDSSYPKFCETGRLLDERLAELGARRLLPRVDCDLDYERLSGPWVEQLVTTAR
                     GQSGAPLIATVTRLRAVQPAPLYSREQPFTAKVLGNQRITGRGATKDVRHIEISLEGS
                     GLIYEPGDALGVWHDNPAAVVDGVLSAAGLDGAEIVAVDGKSLPLREWLTTAREVTRL
                     TRPFIVEHARRSGAADLAEALSPGQDARLRSLLKDLQLTDLFRTWPAEWTGQELVQEL
                     RPLAPRLYSIASSRDAVGDEAHITVAVIDYQSGNEQRHGAASTHLAGLTGDEATARVF
                     IEPNERFRLPADPARDVIMIGPGTGVAPYRGFLQQRKEQGATGRNWLVFGARHFDSEF
                     LYQAEWQEAARKGLLHRVDLAFSRDRTPRAYVQDRLREAGADLYAWLERGASVYVCGD
                     AERMAPDVHAALLDIVATHGRLDREGAQDYLRRLSDERRYLRDVY"
BASE COUNT         3151 a         7174 c         7031 g         2886 t
ORIGIN      
        1 agcaccgcgg gatccatgtc gtggctctgg ttgaacgaca cctggtggat gtcgctgccg
       61 tcggcattca tcacgtgcag cacgaaggcg ggctcgcggc ggtcctcgtc ctgcgcgtcg
      121 aactggggct tgccctcgtc gagcaggatc gccttcgact ggcgctgacg ggtggagctg
      181 aagacgatgc gaccgtcggg caggtaggcc ggcgcgacgt cgttgccttc gtgcgcgacg
      241 aggtcggacg cgatgacgcg atgcaggtcc tggctcgcga ccgtgtattc ccagatctcc
      301 caggtcggct gctcgtcggg cgttgcgccg tcgatgagcg ggcggtgcag cgcgaacacg
      361 agtttcgtcc cgtcgtacga cggttcgacg tcgcgcacgt cgccccggcc ctgggtgatc
      421 gcggccgtca cgttccgctc gctcgcggaa ggcgacgccc ggtcgcgcac ccagaggtcg
      481 ccaccgggcg cgaagtcgag cagctcgcgc gcgtcagtct gcatgaccgg gcccgtgggc
      541 accggccgtt tcacgtaggc gatgggaaag tcgagcacga tcgggtcggg gccctggctc
      601 ccgcccgtcc cgacgccgcc gcctccgcag ccgcccgtga cgagcatgag caggaccact
      661 gcgggtctcg cccagcgatt ccagctcgcg caatcaacga ccacggcagg catccgacga
      721 ccccctgaaa gctgaaggcg cggcccgtgc gccgcgtcgg acgctttttt ctcacaagcg
      781 ggcgtttcct ccacgcggtt cgcgccgtat ccgtgaaccg catctcaaac gcatgcacgc
      841 cgtttggaga tcggggcggc aacgctcaaa tttcgctccc acgaccggac gttccggtcc
      901 ggggggagaa tcggcatgcg catcggaatg cctgcaagtc cttgcgcgac acgccttcgc
      961 gcggccgcga cagcgctatg cgtcgtgctc gcgacgctcg cgagcccggc gctgcacgcg
     1021 gatgcgcgca cgcaggccaa gcgcctgcac gaccggctgg cgggagtgcc cccgaccgag
     1081 accgtgctct ccagcatggc tgccgacatt gccgcgggcc ggcccaccga tgccgcctac
     1141 atcgcgatgg acaaccgggc cttctacgac gtcacgctca agaacttcgc gatgccgtgg
     1201 accaaccgcg accagtccgt gttcgcgccg ctcaacgact acgtcgccac cgtgatcggc
     1261 atggtgcgcg acgacgtgcc gttcgacggc gtgctgtacg acgacatcct ctacgtcggc
     1321 agcggcgtgg cgggcgtgcc cgcgtactcg ccggcctcga acgaccacta cgccgccctc
     1381 gagtcgcagg gcgcgaacct caaggactcg ctcgtgcgga ccacgcagtc cgccgcgtcg
     1441 ggcctgccgc cggagggaac cgcgggagtg ctgaccacgc gcgccgccgc gcaggcgttc
     1501 ttcatcgccg gcaccaaccg cgcgatgttc cgcttcacgc tgatcaatca cctgtgcacg
     1561 gacctcgagc aggtgatgga cacgtcccgg ccgcccgacc gcatacgcca ggacgtgagc
     1621 cggagcccgg gtggcgacag caggctgttc ctcaacaact gcatcggctg ccacagcggc
     1681 atggacccga tggtgcaggc gttcgcctac cacgactggg acgagacggc cggccgcatc
     1741 gtgtacacgc cgggcatcgt ccgtcccaag tacttccaca acaaggacac gttcccgccc
     1801 ggctactcga ctccggacga ccactgggac aactactggc gcgcggggcg caattcgctg
     1861 atggggtggg acgcctcgct gcccggcagc ggccagggcg cgaagtcgct cgggctggag
     1921 ctgtcgcaca ccgacgcgtt cgccggctgc caggtgaaga aggtgtttcg cacggtgtgc
     1981 ctgcgtgacc cgagcgacgc gcaggaccgc gccaaggtgc aggcgatggt cagttcgttc
     2041 aaggccggcg gataccgcat gaagcaggtg ttcgccgagg ccgcgaccta ctgcatgggt
     2101 gactaggggg cgcaatgatg aacacgacga tgcgtgcctg gacattgcgc gccgcggccc
     2161 ttgcagcggt cctgctgctg gcaggctgcg gcggcggtgg cgccaagacc gtggagaacc
     2221 cggtcacttc cggcggcaca cctgccgcct acagcggccc gccgccggcg acggccgaca
     2281 tccaggcgtt caagatcaac gtctgggaca acctgcgggc caccaatcgc tgcggcagtt
     2341 gccacgtggc cggcggccag gccccgcagt tcgtgcggtc ggacgacatc aatctcgcct
     2401 acgcggctgc gaacaccgtg gtgaacctcg cctcgccgcg cgactcgcgg atggtccaga
     2461 aggtcgccgg cgggcacaac tgctggctcg cgagcccggc cgcctgtgca gacatcctca
     2521 cgacctggat cagcaactgg gccggcacgg ctgccggcgg cggcaccagc ggcatcacgc
     2581 tgacggctcc gccgctgcgt gaccccggtg cgagcaaggc tttcccggcg tcgcccgcgc
     2641 tgttctcgac gaccgtctac ccggtcgtcc ggcagtactg ctcgcgctgc cacgccccgg
     2701 ccgcagcgag cccgcaggcg ccatatttcg catcggcgga cctcaatgag gcctacgccg
     2761 cggtcaaggt caagatcgac ctcgaccgcc ccgagcagtc gcgcctcgtc gtccggctgc
     2821 gcagcgagtt ccacaactgc tggagcaact gcgccgccga cgccgacgag atggaggcgg
     2881 cgatccgcgc gttcgccgac caggtcccgg cgacccaggt cgacccgtcg ctcgtgctgt
     2941 ccaaagcact cggcatgtac gacggcatcg tcgcaagcgg cggcaaccgc tacacggcca
     3001 acctggtcgg cctgtgggaa ttcaagaccg gcaagggcac cgtcgcctac gacacgagcg
     3061 gcgtggaccc ggcgctcaac ctcacgctgt ccggggacgt gacctgggtc ggcggctggg
     3121 gcatcaacgt caagagcggc aaggcgcagg gctcgacgac cgcgagccgc aagctcacga
     3181 cgctgatctc cgcgacgggc gagtacagca tcgaggcctg ggtggcgccc ggcaacgtcg
     3241 cgcaggagga cgcgcgcatc gtctcgtact cgggcggcac gaccacgcgc aacttcatgc
     3301 tgggccagac gctgtacagc tacgacttct acaaccggtc gaccagcacc gggccgaatg
     3361 gcgatcccaa gctcacgact gccgccgcgg acgaagacct gcaggcgacg ctgcagcacg
     3421 tggtcgtgac ctacgacccg gtgaacgggc ggcgcgtcta cgtgaacggc cagtccacgg
     3481 gggacgtcga cccggtgtcc gccggcacca tcgccaactg ggacgacacg ttcgccttcg
     3541 tcctcggcaa cgaagtgtcg ggcgaccggc agttccaggg cgtgttccgg ctggtggcgg
     3601 tctataaccg ggcattgacg caggagcagg tacgcacgaa ctacgccgct ggcgtcggcg
     3661 agaagtacta cgtgctgttc tcggtgagcc acctggtgaa cgtgccgcag tcgtacatca
     3721 tgttcgaagt gagccagtac gacagctaca gctacctgtt caacaagccg acgttcatca
     3781 gcctggaccc ggccgcgcgc cccggcacga taccgatcaa gggcatgcgc atcggcgaga
     3841 acggcacgga gcccgatgtc ggccaggcgt accggacgct ggacacgagc atcaccgact
     3901 ccaactacgg gccgctcggc cagccgctgt cgagcatcgg caccgtgatc ccgctgcaga
     3961 agggcccgga ttccgacgag ttcttcctga ccttcgacgt gctcggctcg cacagcaacg
     4021 tgcggctcga cccggtgccg ctggcgccgc cgccgctgcc ggacgtccgg cgcccggcgg
     4081 acatcggact gcgggccttc gaccgcatca acgccacgat gtcggtgttg accggcgtga
     4141 gcacgcaggc gccaggcgtg aagtccacgt tcgacaccgt gcgacagtcg ctgccggcgg
     4201 tggccagcgt cgacaccttc gcctcggcgc acccggtggc gatcgcgcag atgtcgatcc
     4261 agtactgcaa ctcgctggtc gacgacacga ccgcccgggc gcagtacttc ccgggattca
     4321 acttcaacgc agcgccggcg acggcgttcg acccgacggg tcgcgcgatc gtgctcgact
     4381 cgctggtcac ccacatgatg cgcgccaacc tcgccacgga gcccgacccg acggccgtcc
     4441 gcggcgacct cgacgcgctc atcacccgcc tcaccgcgtg cggctcgagc tgcccggcgg
     4501 ggcgcacccc gaccgtggtc aaggcgacgt gcgctgcgct gctcggcagt gccgtcacgc
     4561 tgatcgaatg aggacgctcg agccggccat gaggagcatc gcatgatcat ccgcaggaaa
     4621 atcccgcgcc ggctggcgct cgacgagccg atccgccacg agctcgcgca caagcggccc
     4681 ctgacgcggc gcgacttcat cgcgcagggc ttcacggccg ggctcggcac ggtcgtggcg
     4741 cccacgatct tcagcctgtt cgcgaacccg cgcgcggcga tggccgcgct gtcgcccgac
     4801 ctcgaggcgc tcaaggcgtc gtgcggcatc caggtgcagg gcgcgggcaa gatcccgttc
     4861 atctgcatcg acctcgccgg cggcgccaac atggtcggtt cgaacgtgct gatcggcggg
     4921 cccggcggcc agctcgattt cctgagcacc gacggctatg ccaaactcgg cctgcccggc
     4981 gacatgctgc cctcgatgac caataccgcc accggcacca gcttcaccga cacgcggctc
     5041 ggcctcgcgt tccactccga cagcgcgttc ctgcgcggga tcatggaccg cgtggcgccg
     5101 gcgaccgccg cgtcgatcaa cggcgccgtg atcgcggcgc gctcggagaa cgacaccggc
     5161 aacaacccgc acaatccgat gtacgggatc taccgggccg gcgcgaatgg atcgctggcc
     5221 actctcatcg gctcgagtcc gtcggattcc ggcggcaatt cgctggcgcc ggcggggatg
     5281 atcgacctgt cggtgcgccc gaccaaggtc gaccgggcga gcgacgtgac gggactggtc
     5341 gacacgggcc agctcgcgag cctgctcagc gtcagcgaca ccgtggccgt catggaagcg
     5401 gtgcagcgca tcagcgcccg caagatggcc gtgatggacc cgaagcagcc gtgggccgat
     5461 ccggtgaaga cgaccgtgac ccgcgacgaa gtggtgcggg agctcgtttc ctgcaactac
     5521 ctgaaggccg ccgacgtcgt cgaccggttc ggcgacccca gcagcctgaa cccggacctc
     5581 gatcccgaca tcgtcggcgc cggcggcatc ttcagcccgg ccgagtacaa cggggactcg
     5641 gagttccgca agaccgccgc ggtcatgaag atggtgctgt cgggctacgc cggggccggc
     5701 acgatcacca tgggcggcta cgactatcac ggccagggcc gctcgaccgg cgagctgcgc
     5761 gacttccgcg cgggccgctg catgggcgcg tgcctcgagt atgcggcgcg tcgcggcatg
     5821 ccgctcatgc tgtacgtgtt ctcggacggc tcgatctccg cgagcgggca ggtcgacgac
     5881 acgatcgacg gtcgcggcaa gttcatgtgg accagcgaca accagtcgac ggccgcgtcg
     5941 ttcttcctcg tgtacaaccc gcagggcagg ccgtcgctcc tgagcagtgg ctccggcggc
     6001 accgcgcgcc accagcagat cggctatttc cgcccggatg catcggtcga gaccgcctcg
     6061 agcccggcgg ccaacaacgt caacctgctg gtcgagaccg tcgtgctgaa ctacatggca
     6121 ctgcacggcg agcaggggca gttctcgcag aggttcccga ccaatggcct gggctcggct
     6181 tcgttgcagg acagcctgat cgcgttccag ccgatcgtga gcggcaccat cggctgagcg
     6241 ccgcgccatg gacatggccg tcggcgtcga tcactggaca ttgcttgcct gcccgcgctg
     6301 cggcgggcac ctggcaccag gcgacgagcc ggcctgcagc gcgtgcggtg cgcgataccc
     6361 ggtcgtggac ggcctgccgt ggctgtgcgc cgacccggcc gcagcgctcg gcggctggcg
     6421 cacacgcgca cggtccctcg tgtcggcgct cgagcagcag tcagcgcgct acgcttcggc
     6481 gatctccccc ggggaaactc gtgccgccgc tcgacgacgc ctcgaactgc tggcgaatgc
     6541 ctgcaatgac cacgcgcggc gcctgaagtc cctgctggcg ccactcgagc tcgctgctgg
     6601 tgcagcggct cccgagacct acgccgcact cggcgcaccc ctgccggcgg ggcagggact
     6661 cacgagctac tatgccaacc tgcaccggga ctggtgctgg ggagcggcgg aaaacgaagc
     6721 gggctattcg gcggtggatc gcgccctcgg tcccggaaac ccgggtcgca cgctggtgct
     6781 cggctgcggc ggcgggcgcc tcgcgtacga cctgcacgtc cggcgacgac cggccgtcac
     6841 cgtcgccgcg gacttgaatc ccctcatgct cgcggtcgcg agccgcatgt tcgcaggcgc
     6901 gcaagtcgaa ctccatgaat tcccgctggc accgcgcgat ctcgcgagcc acgcggtcct
     6961 gcgcgagctg cgtgccccgg cgcctgcacc gcccggactc tggccaatcg tggcggatgc
     7021 atcccacgcg ccatttgcgc aagcggcgtt cgacaccatc gtcacgccgt ggctcgtcga
     7081 catcctcgac gaggacttcg ccgtcgtcgc agcccgcgtc aaccagtggc tgcgacccgg
     7141 cgggcgttgg gtgaacagcg gctcgtggtt cttccagcag ccggacccgg cacgatgcta
     7201 ttcgatcgac gagctgccgg aactgctgca cgcagccggc ttctcggacg tgtcggtgca
     7261 cgaagaggtg gtcccgtacc tggcatcgcc ggccagcagc caggcgcgca tggagcggct
     7321 cgtcacgtgg agcgcgatcg cgcaggcgca cgtgccggcg ccaccgccgc accgcaattc
     7381 ccccgcctgg ctcgagaggg ccgaccttcc cctgccgctg ctgccgcagc tcgagtcgca
     7441 cgtgctggcg ctgcgggtgc acgcctacgt cgcgtcgctg gtggacggcc ggcgcacgct
     7501 ggccgagatt gccgcgatac tggtgaatga tcggctgatg cgggccgacg aggcgctgcc
     7561 ggcggtgcgc gccttcctgc agcgggtgta ttcggacgcg cagccggcgg tgcgaccgtg
     7621 acggagtcgg ccggcggcgg ttggcacgag cgcgaggcag cgatcatggg cacgcggatc
     7681 gccgtgcagc tctgggccga ggacccggag ctggcgcggc tgggtatcga ctccgtgatc
     7741 gcggagatgc accgcgtcga ccgcctgatg agcacgtaca agccggagag ccagctctcg
     7801 caggtcaacg cgcacgccca tgagcggccg atcaccgtgg atcccgacat cgtcgacgtc
     7861 gtcgcgaagt cgttcgagta ctcccggctg agcgacgggc tcttcgacgt cacctacgcg
     7921 agcgtcggtc acctctacga ctatcgcgcc ggacgacacc ccagcgatgg cgagattgcc
     7981 gccgcactcc ccgcggtgga ctatcggcag gtcgaggtgg accgagagcg gtgcacgatc
     8041 cgtttcctga agcccgggat gcgcatcgac ctcggcgggt tcgccaaggg atgggccgtg
     8101 gaccgcggcg cggcgatcct gcgctcgctc ggcatccgca atgcgatggt gacggccggc
     8161 ggcgacaccc ggttcgtggg cgaccggctc ggcaagccct ggatcgtggg catccgcgac
     8221 ccgcgccgcg agggtgcggt ggtcacgcgc ctgccgctcg tcgacgaggc ggtgtccact
     8281 tccggggact acgagcgcta cttcgagcag gacggcgtgc gctaccacca catcctggtg
     8341 ccgggcacgg gaagatcgcc agcggcggtg cgcagcgtca cgatcatcgg acctacggcg
     8401 acccacacgg acggcctcac caagcccgtc ttcatcctcg gcgtcgagcg cggcatggaa
     8461 tacctgcggc gcgtcggcga cgtggacgcg gtgatcatcg acgccgaggg cgccgtctac
     8521 tactcaccgg gcctggagcc gcgcggctag ggctggcggc ccgtccgcgc attttttgtg
     8581 acgcactgcc gggtgcgcgc gatccgttcg cggcgcgatt tgacacgcgg cccggcccag
     8641 acaacgatgc aagtcgactg aaaaaggcaa acccgtcgaa agacggggac gcaaagcttc
     8701 cggtcttcgg gtagtgccca tgacagcggg gttgccagga tgcgaggcca cgatcattac
     8761 gcgcgcgccc gaacggaggc gctgatccac acgctggcga ttgcggcggc ggtgatcgcc
     8821 ggcgcattgg cctttccact cgcggcccgg tcgtcgacgt cgcccgtgac cgtcggtgca
     8881 gccgccccgg atttcacgct caaggccctc gacgggcgca acctgcggct ctccgagtac
     8941 cgcggcgacg tcgtcgtgct caccttctgg gccagctggt gcggcgcctg tcgcccggca
     9001 ctcgaggcgg ccaattcatt cgccgccgcg cagggcaagg acggcgccgt cgtgctgggc
     9061 gtcagcctcg acggggatgc cgcgcgtgcc cggtcggtgg ccgagtccct ggccctgaag
     9121 tatccgacgc tggtcgacgc gcgccaggcc gtcggccgcc tctacgacat cgatgcgctg
     9181 cccttcacgc tggtcgtcga tcgcgacggc atcgtgcggg gcgaatggtc cgacggggtc
     9241 gcgccgcagg cagaccttgc acgaacggtt gcgggagtgg ggcaatgaat cgtcgataca
     9301 acgccgcggc gcgccgccgc atgatcgagt gggccggcat gctgctggtc gccggtggcg
     9361 tgctgtgcgc cgggcgggcg ggcgcggaag acccggcgca ggaaacgcgc gccgtggacc
     9421 aggacgtgca ggacctgaag aagcagctgg tcgacctcaa ccgcgacctg ttcaagctgg
     9481 aggaggagat cctctacccg gcgagcacgc aggtggccgt gttcctggcc gtcaacaccg
     9541 gcacgttctt ccagctcgac tccgtgacgc tcaagatcga cgacaaggaa gtcgccaact
     9601 acctgtacac cgagcgcgag gccgcggccc tgcggcgcgg cggcgtgcag aagctgtacc
     9661 tcggcaacct gaagtccgga tcgcacgagc tggtcgcgct cttcgtgggc aagggcccgc
     9721 acgacgtgga ctatcggcgc ggcgccacgc tcaccttcga gaagacggtc ggcgccaagt
     9781 acgtcgagct gcgcatcagc gaccgcgagg cgtcaatgca gccggagttc gtcgtccggc
     9841 agtgggatta gccggggctg cggtgcgccg atggggggag tacagcccag cacctttgtg
     9901 acgcacgcgc gccggcgggg acggtggcgt gcgcctgtcg tcttgctgtg cgcgctggct
     9961 gtgacgcgcg ccctggccgc gggcccggag gccggcgacg cgccggccac gaagatccgc
    10021 gacctcgact acggcgacgt gctgttccat ttctaccagg acgactactt cgggtccctg
    10081 gttcgcctcg aggctgcgag cgacctgagt cgcatgccgc accacgatgc ggaggcgcgt
    10141 ctgctggcgg gaggcctgag cctctcgctc ggcatgtatt ccgaggcgac gcgcatcttc
    10201 gactccctgc tgaatgcgtc ggtcccgcag tcggtgagtg atcgcgcgcg gttctacctc
    10261 gcgcgcatcg gctacgagag aggctcgccg gaggaggcgt ggaggaacct cggccaggtt
    10321 cgagggacgc tgccggcgcc gctcgagtcg gagcggcgcg tgctcgccgc caacgtcctg
    10381 atggcgctcg ggcgctatgg agaagcggcg gcgctgctca ggaaggacac gacggaagac
    10441 gattggaccc cctacgcgcg ctacaacctc ggagtcgcgc tggtgcgctc cggcgatccg
    10501 gaacagggac agcagtggct ggcggccgtc ggcacgatgc cggcgcagac cgacgagcag
    10561 cgctcgctgc gcgatcgcgc gaacctggcg ctgggctatt cgctgctgca gcagcgggcc
    10621 gccgatcccg ccgtcgcggc gctgtcgcgc gtcaggctcg acggtccgta cacgaatcgt
    10681 gcgctgctgg gtcttggctg ggccgaggcc gatgccaagc gtcccgaccg cgcgctggtc
    10741 ccgtggctcg agttgcggca gggccggttg ctggattcgg ccgtgcagga gtcgctgctg
    10801 gccgtgccct acgcgtacgc ccagctcgcc tcgaacgggc aggcggcgca gcagtaccgc
    10861 tacgcggtgc agtcgtacgc cgaggaagca aagcgcatcg accagtccat cgcggcgatc
    10921 caggacggcg cgttcctcga ttcggtgctg gacaaggctc ccgagggcga cttcgcgggc
    10981 tggacctggc agctgcagcg catcccggat gcgccgcaga cacgttacct ctaccacctc
    11041 ctcgcatcgc acgagttcca ggacgggctg cggaattatc gcgacctgcg cgtcatgcag
    11101 cgcaacctcg ccggctggcg ccagaccctc gaggccttcg acgacatggt ggcgacacgc
    11161 gagcaggcgg cgcgcgagcg cgagccgctg cgcgagtcga ccatggcggc tgccgacatc
    11221 gacgggctgg cagcgcggca agcgatgctc cagcagcgcg tcgcggacat cgaaggcagt
    11281 cgcgacgtgg tcgcgctggc caccgtggac gaggctcgcg agtggcacac gctccaacag
    11341 gcgcaggctc gcatcgcggc gcttccgccg ggcccgcagc gcgacgctct ggtgcagcga
    11401 gcgaacctcc tgcaaggcgt gctgttctgg caaatggacg ccgcctacaa ggtgcgcctg
    11461 cgggcggcca aggtcgagct gcgcgagatc gattccctcc tcgcatcggc acgcgacggc
    11521 cagcaacgag tcggcgcggc aagtgcagcc gcgccgcgcg acaccgcggg ctttgcggac
    11581 cgcatccagg cgcttgcggc acgggtgaat gcgatgggac cgcggatcga tgccgccgcg
    11641 gtcgcgcagc agcgcgtgct ggtcgccatc gcgaccgacg aactcgaggc gcagaagcgg
    11701 cggctcgcca gctacgcgac gcaggcgcag ttcgcgctcg ccgccatcta cgacggcgcc
    11761 accgcgggag tcgtgcgatg agcgctcgca tcgtcatcgc catcgcgtgc gtctgcgcat
    11821 cggtccctgc ggccgcgcgg acgcgctcgg ccgaacccgc gacactgggt gacctccagt
    11881 cgcgcagcgc gcccgtgagc cgtgacgtcg ccgtggaggc ggatccgggc ctcgccacac
    11941 gcagctacga ggatttcctg cgcatcccgt cgacggatgc cggcctgcga gcgcaggcgc
    12001 tgcgcaggct gggcgacctg cgcctcgccg aatcggaggc gctgcgcgca cgtgacggaa
    12061 ccgagtcccc ggcggcccag gaagcggatc ggcaggccat cgcggcctac cggcaactcc
    12121 tggacgagca gcccgacgcg ccgggaaccg atgccgtcct gtaccagctc gcgcgggcct
    12181 acgaaggcct cggcgagtcc gccgcagcgt tcacgacgct cgaccagctc gtcacgcgct
    12241 atccgcagag tccgcactac gacgaggcgc agttccgtcg cggcgagacg ttcttcgccg
    12301 agcagcgcta tgccgatgcg gatcgcgcgt acgccgcggt gcaggcgctc ggcccgtcct
    12361 cggagttcta cgagcaggcg gtctacaagc gcggctgggc gctgttcaag cagtctcggg
    12421 atacggagag cagcgccgag tttctcgtgc tgctcgacca cgttctggtt cgcgacggcc
    12481 gcttgcgacc ggcagcggac atctcgcgcg ccgaccagga gctcacgagc gacgcgctgc
    12541 gcgcgctggc catcacgttt gccgcgaacg acgcgatcga gacgctgcgc gaggtgctcg
    12601 atcgccacgg cccggcgccg tacgagtcgc gcctctacgc gtcgctcggc gacctctacg
    12661 tggagaagga gcgctaccag gacgcggcgg aagcctaccg tgcctatgcc acgcggcagc
    12721 cggacgatcc cgaggccgcg ctcatgatcg tgcgggccac ggatgcgtac tctcgcggcg
    12781 gcttcccgag cctggtgctc gacggcaagc ggcaactcgt cgagcagtac gggccgcaga
    12841 gcgactactg gcgcaggcac gcgccggacg tcgacccgcg ggtgagcgat gccgtgcagg
    12901 cctgcctgct cgacctcgcg cgccaccatc acgcgctggc ccagaagggc tcggccgccg
    12961 accgcgacct cgcgatacgc tggtaccgcg actacctcgc gggtttcgat tcgtcgccgc
    13021 aggcgccggc gacgcgcctg ctgctggccg acctgctgtt cgaaggtggt cgctacgagg
    13081 aggcggccgg cgagtacgag cgcgtggcct attcgtacgc atccagtccc gacgcggcgc
    13141 acgcgggcta tgcggcactc gtcgcctggc agcgtgcggc ggagcaggcc ccagcctccg
    13201 cgcgggccgc gatcgacgcg aagggcatcg actcctcgct gcgcttcgcc ggcacgttcc
    13261 cggacgaccc cgaagcggtg gcggtcctga cgcgcacggc gaaggcgttg ttcgacgcgg
    13321 gcgatcgcaa gcgcgccgag ccggtggccc agcaggtgct ggcctacggg gcgcgtgccg
    13381 acggcgcgca gcagctggtg gcgtggaccg tcctcgcgca cacctggttc gacgacggtc
    13441 gctatgccga cgccgagcgc gcctatcgcg agctcgcatc ccggctgccg gccggcgacc
    13501 cgcagatggc ggaagtcacc gagcgcctcg ccgcgtcggt gtatcggcag gccgaggcgc
    13561 gccaggcggc gggcgatacg caaggcgcag tccaggaatt cctgcgcgtc gtcgcggtcg
    13621 cgcctgcctc gtcggtgtcc gccaaggcgc aatacgacgc tgcgacgctg ctgctcgact
    13681 cgaagcagtg gtccgccgcg gccgacgtgc tccagagctt ccgggcggcg cacccggacg
    13741 acgcactggc gcccgaggcc acgcgcaagc tcgccgtggc gtacatggag gccgggcgcg
    13801 ggcgcgatgc ggccgtcgaa ttcgagcggg tcgccgacga cggcaaggaa gaccaggccg
    13861 tgcgccgcgc ggcgcagtgg caggccgccg agctctacgc cgcggccggc gacgtgcctg
    13921 cggcgagccg ggcctacgcg aattacgtgg agcgcttccc gtcgcccgcg gacaccgcga
    13981 tcgaggccag gcaggaactc gccgcgctcg cgtccaagtc gggcgatgcg caggcacgcg
    14041 agcactggct gaaggaaatc gtcgccgccg accaggcggc ggggtcggcg cgcaccgatc
    14101 gcacgcgact cctggctgcc caggcgtcgc tcgagctcgc gcgcccgctc gaccagaccg
    14161 cgcgcggcat cgcgctggtc gtgccactcg atcgcgctct cgcccgcaag aagtcggcga
    14221 tggagtcggc gcttggcgcc tactcgcatg ccgcggacta cgggatcgtc gaggtcacga
    14281 ccgccgccga ctacgccatg gccgacctgt atcgcgacct ggcgaaggcg ctgctggcct
    14341 cggagcgtcc gcgcgggctc aacgccgagg aactcgagca gtacgacctg ctgctcgagg
    14401 agcaggcgta cccgttcgag gaaaaggcca tcgcgatcct cgagtcgaac gcgcgccgag
    14461 ccgcgaccgg cacctggaac gagtgggtgc gcaagagcta tgcggcgctc gccgaaatga
    14521 agccggcgcg ttacgcgcgc accgagcgcc tggatgcgcc cgatgccgcg ccagggaccg
    14581 cgccggacgt ggccctggca ctgaccgcgg cgcggaccgc gatcgaggcg ggcgacagcg
    14641 ccgccgcgat cgaggacgcc gacgcggcac tcaagctcga cccggcgaat gcggtcgcgc
    14701 acaacatgct gggcgtggcc tgccgcaatc tcggcaggtt cccggaggcg cgcaccgcct
    14761 acgagagcgc catcgcgcag gcgcccggcc acgcgacgcc gcaacgcaat ctcggcgtgc
    14821 tgctcgacct gtacctcggc gacccggccg cggcgctccc gcattacgag cgctaccagg
    14881 aactgaccgg cggctccgac cccgagaccg ggccgtggct cgtcgaaatt cgtacgcgcc
    14941 tcggacaggt gcaacgcacg gcggaggcga atccatgaag cgcatgacca tgctggtggt
    15001 cgccctggca ctcgccgcgc cggccgtggt gcgcgccaac gaaccggcgt ccacaccggc
    15061 gtccacgccc gcgcgcagcg cctcgccggc caaggccgcg aagcccgcaa cgcctgcgac
    15121 ggtcgctcct gcgacgcagg ttgccgccac cacaggcaac cgcgcccacg accgtctcac
    15181 gctcgattcc accgccatca ccggcaaccg cgagttgccc aaggtgatga gcatcgtgcc
    15241 ctggaaggag gcggaagcgc cccagggccc ggaccgaccg atgggcagcc tcatcgagga
    15301 ggtcctgtcg ccgatcgacc gtgccgagtt ccagcgcgag gtcgtctact accgtgacct
    15361 caccgccccg aagtccgtaa ctcagagtcc tacccccgga gccaccaagc catgaacgcc
    15421 atagacctcg tcgcaaattt cttcaagcag ggaggcgtgt tcctctaccc gatcgcagcc
    15481 gtgttcgccg tgggcgtcgc ggtcgccatc gagcgcttca tctacctcgg caaggtgcag
    15541 cgcggcaacc gccgcctgtg ggccgagctt gccccgctca tccaggccgg caatttccgc
    15601 aaggccatgg aggtcgcgag ccgcaccgac tcggcgctgg ccaccatcat gcgctacggc
    15661 ctcacccgca tctcgacggc gcgccgccgc gacgacatcg agaaggcgat ggaggagagc
    15721 ctgatcgaag tgatcccgcg gctcgagaag cgcacccact acctcgcgac gctcgccaac
    15781 atcggcatgc tcatgggcct gctcggcacc gtggtcggcc tgatcaacgc gttcgcggcg
    15841 gtggcgtcgg tgaacccgtc ggagaaggcg gcgctgctgg ctgcgagcat ctcggtggcg
    15901 atgaacaaca ccgcgctggg cctggggctc gcgatcacgc tgctgctgag ccacatgtac
    15961 ctcgagaccc gcacgaccga gctcgtcgac agcctcgagg tcgcgtcgat caagttcctg
    16021 aattcggtca ccgagcggcg tgacccggcc gccgacaagc tcgacgagtc cgacgcgtcc
    16081 gcagtggtcg cacgcgcccg cgcccgggac gcgctcgcgg gagcggtacg cgcatgaaga
    16141 gctcggcgct cgcgcgccgc cagcagcgca accacgcgcg ctaccgcggc cgcaatgacc
    16201 tgaacatcgt gccgatgctg gacgtcatga ccatcctggc gttcttcctg atcttcacgg
    16261 ccgtgttctc gaagacgagc gtgctcgagc tggacctgcc gggcccgggg gcccgtgcag
    16321 ccgccacggc tccgccgatc gaactcgagg tcatcgtgac gcgcgcgcgc atcgacatcg
    16381 ccgaccggtc ggtcggcgtg ctgacgagcg tgccggcgac cgcgaacggc caggacgtgg
    16441 cacgcctctc cgtcctgctc ggccagatca aggagcgcta cccggccgtg gactccgcca
    16501 cgctgctggt ggccgaggac gtggactacg acacgatcgt gcaggtgatg gacgcggtgc
    16561 gggtgcggca ggagaccgac ggtccccgcg tcacgcgcac cgaactgttc ccgcacatct
    16621 cgatggggga cgcgccgaca tgatacgcac cggactcaag cgccatgcgc ggcacggtcg
    16681 cgccggggga gggcacgggc cgctcgcgct cgtgcccatg atcgacatgc tcacgatcct
    16741 ggtcgtgtac ctgctggtgc acgccgcgga caccgagatc ctgccgaaca cgcgcaacat
    16801 ctcgatcccg cagtcgatct ccgagcagaa gccgcgcgag gcgacggtcg tgaccgtcac
    16861 ccgcgacatg ctgtacgtga acggcgaggc catcgtgtcg gtcgccgatg tgggcaggtc
    16921 ggccgagccc gtggtcgagc cgctgcgggc tgcgctggcc cggcagccgg ccaacctgct
    16981 gggaggcaag gacggccagc acgaagtgac ggtgatggcg gagaagtccc tgccgtacgc
    17041 cgtgttgcgc aaggtggtcg cgagctgctc ggcggccgac tattccaagg tgtcgctcgc
    17101 cgtcgtcgag cgcgaacagg cgctcgtggc cagcatcggc gcctgaggac gaatccgcat
    17161 gagcaccgcc atcgccgtca atttccgcat ctacgacctg ccgtggacgc cgtccgcggt
    17221 cgacgagcgc cgcttgcggc gcatcgtggg tgcggcgctc gggctgttga tcgcgttcgg
    17281 catcgtgatt ccgctgctgc cggagcgacc gcgcgaccac acggtgccgc ccgcgatccc
    17341 cgatcgcatc gtcgagttcc tgatcgagcg gccgaagccg ccgccgaagc ccgagcccaa
    17401 gatcgagccg ccgccgacgc ccgtcacggc gcccgcgatc gagcacccgg cgccggtcaa
    17461 ggcccctgtc gtgcagtcgg agccgaagcc cgatccgcgc aagaaggccg cgtcggccgg
    17521 gctcctcgcg ctggccgacc agctgcagga gttgcgcgac ctttccgtcg aggaccgtcc
    17581 cgacgctcgc ccgctgaatg cggggagcgg cgacaaggcc cgggtggatc gcgcgctgct
    17641 cacggcccgc acgggcgaag gcagcggtgg catcacggtc ggccaggcga gccacggttt
    17701 cggcggcggt gcgacgggcc tgaaaggcat ctcgaccgcc caggtgtccg cgggtgcgga
    17761 ggcttccgct gatccgggcg tccagcgcgc cggtaagagc agcaaggccg cgcgcacgcg
    17821 cgaggagatc gagctcgtct tcgaccgcaa caagtcggcg atctatgnnn nnnnnnnnnn
    17881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17941 nnnnnnnnnn nnnnnnnnnn nnnnnnncgc acgcgcgagg agatcgagct cgtcttcgac
    18001 cgcaacaagt cggcgatcta tgcgatctac agccgggccc tgcgcgacaa tcccgcgctg
    18061 cagggcaagg tggtgctcga ggtgacgatc gccccgtcgg gcgaggtcac ggactgccgg
    18121 atcgtctcga gtgacctcgg cgacgcggac ctcgagcaca agctgatcgc ccgggtgaag
    18181 atgttccggt tcgaggccaa ggacgtggcc cccatgacga cgaccaagcc gatcgagttc
    18241 ttcccggcct gagtcatgag cagataacca cccggccttt ccgctcgggg catgcccgta
    18301 cataggatcc accgcaggcc cagcctgggg ggatcccgtg agcaccagtt ccaccgcatt
    18361 gccgttgagc ccgctcgacc cggagcgcaa tgcgctactg caccgggtgg tcgacgggct
    18421 cgatcccgca tcgctgcatt ggttgtccgg attcgcggcg ggcgtggcac acgcccgcgc
    18481 gggcggcggt gccgccgaag tgttggcacc gcccgctttg ccgggcattc ccgtggcggc
    18541 gcggtccgag ccggtggctc gacttgccgt cgtgtatggc agccaatctg gcaacagcaa
    18601 gcgcgtcgcg gaccgactcg gccgggtggc cgaggaggcg ggccttgccg tgcgcgtcta
    18661 cgcggcccgc gactaccccc ttaaggacct cgcgaaggag cgctacctca gcgtcgtgat
    18721 cagcacccag ggcgacggcg atccgccgga cgatgcgcgc tcattcgtgg agcacgtcac
    18781 cggcaagcgc gcgccgcgcc tcgagaacct ggcgtactcg gtgctggcgc tcggggactc
    18841 gagctatccg aagttctgcg agacgggtcg cctgctcgat gagcgcctgg ccgagctcgg
    18901 cgcccgccgc ctgctgccgc gtgtggactg cgacctggat tacgagcgcc tgtccggccc
    18961 ctgggtcgag cagctggtca ctacggcacg cgggcagtca ggtgcgccgc tgatcgcgac
    19021 cgtcacccgc ctgcgcgccg tgcagccggc gccgctgtac agccgtgagc agccgttcac
    19081 cgcgaaggtc ctcggcaatc agcgcatcac ggggcgcgga gcgaccaagg acgtgcggca
    19141 catcgagatc tcgctggaag gctcgggcct gatctacgag cccggcgacg cgctgggcgt
    19201 gtggcacgac aacccggcgg cagtcgtcga cggcgtgctg tcggccgcgg ggctcgatgg
    19261 cgccgagatc gtcgccgtcg acgggaagtc gctgcctttg cgcgagtggc tcaccacggc
    19321 gcgcgaggtc acgcggctca cccgtccgtt catcgtcgag cacgcacgtc gttcgggcgc
    19381 cgcggacctc gccgaagccc tgtcgccagg ccaggacgcg cgcctgcgca gcctgctgaa
    19441 ggacctgcag ctcacggacc ttttcaggac ctggcccgcg gagtggacgg gacaggagct
    19501 cgtgcaggag ctgcggccgc ttgcgccgcg gctgtactcg atcgcgtcga gccgcgacgc
    19561 cgtgggcgac gaagcgcaca tcacggtggc ggtcatcgac taccagtccg gcaacgagca
    19621 gcgccacggc gcggcgtcga cacaccttgc cgggctcacc ggcgacgagg cgacggcgcg
    19681 ggtgttcatc gagccgaacg agcgcttccg gctgccggcc gacccggcgc gcgacgtgat
    19741 catgatcggg cccgggacgg gcgtggcgcc gtaccgtgga ttcctgcagc agcggaagga
    19801 acagggcgcg acgggccgca actggctcgt gttcggtgcg cggcacttcg acagcgagtt
    19861 cctgtaccag gcggaatggc aggaagccgc acgcaagggc ctgctgcatc gggtcgatct
    19921 cgccttctca cgcgatcgca ccccgcgcgc ctacgtgcag gaccgcttgc gcgaggccgg
    19981 ggccgatctc tacgcctggc tcgagcgtgg cgcgagcgtc tatgtgtgcg gtgacgccga
    20041 gcggatggcg cccgatgtgc atgcggcgct cctcgacatc gtcgccaccc acggccgcct
    20101 cgaccgcgag ggcgcccagg actacctgcg gcgcctgtcc gacgagcgtc gctacctgcg
    20161 ggacgtgtac tgatcatggc cggaccgtct cccgtcgagc gcatcaagag cgagagccgg
    20221 ctgctgcgcg gcacgctggt cgagagcctg gccgatccgc tgaccggagc gatccgcgag
    20281 gacgacacgg cgctcatcaa gttccacggc agctaccagc aggacgaccg cgacgtgcgc
    20341 ga
//
LOCUS       DAOMFD010000019        19903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_498_length_19903_cov_35.265316, whole genome shotgun sequence.
ACCESSION   DAOMFD010000019 DAOMFD010000000
VERSION     DAOMFD010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19903
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_498_length_19903_cov_35.265316"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..425
                     /locus_tag="PL152_02265"
     CDS             <1..425
                     /locus_tag="PL152_02265"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HQR48129.1"
                     /translation="GTTAAGRKVITDFLASQGISLPDLVLDNGSGLSREERITAAGLA
                     DVLLAAWRSQYMPEFAASLPLSATDGTLRRRFRSPEMQGRLRMKTGTLDGVSALAGYV
                     NAASGRTFVAVIIVNHPAANGGGGLAVQAALVQWLFGQ"
     gene            512..931
                     /locus_tag="PL152_02270"
     CDS             512..931
                     /locus_tag="PL152_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HQR48130.1"
                     /translation="MAYDSGNVFARILRGEIPAHTVYEDAQTLAFMDVMPQSDGHTLV
                     IPKTQAENLFDLPADALVATILTTQRVARAVKKAFDAPGMMIAQLNGRGAGQSVFHIH
                     FHVVPRYHGIDLKFHAREMADPKLLAEHAAKVRAALD"
     gene            966..2903
                     /gene="acs"
                     /locus_tag="PL152_02275"
     CDS             966..2903
                     /gene="acs"
                     /locus_tag="PL152_02275"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003987 - acetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019427 - acetyl-CoA biosynthetic process
                     from acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQR48131.1"
                     /translation="MTKLHEVPAGFAARARYRRDDYERLYAESVRDPEGFWGRMGQRL
                     AWMRPYTRVRDVNFDANAFRIRWFEDGRLNVSANCLDRHLAAHGDKAAIVWEGDDPAE
                     SETITYRELYYRVCKLANALRQLGVQKGDRVTIYLPMIPEAAVAMLACARIGAVHSVV
                     FGGFSPDSLAGRIADCASTVVITADEGIRGGKRIPLKANVDQALTAPGTETVKHVLVV
                     KRTASPVAMYQRDRWYHELVDGQPEQCEPVSVGAEDPLFILYTSGSTGKPKGVLHTTG
                     GYLVYVSLTHEAVFDLHEDDIFWCTADVGWVTGHSYVVYGPLANGATTVMFEGVPNYP
                     DASRFWQVCDKHSVTIFYTAPTALRSLMREGEAPVKKCSRKSLRLLGTVGEPINPEAW
                     EWYHRVVGDGRCPIVDTWWQTETGGVLITPLPGAIAQKPGSATLPFFGVQPAIVDNEG
                     RVLEGACEGNLVITDSWPGQMRTVFGDHQRYVDTYFKAFPGSYFTGDGARRDEDGYYW
                     ITGRVDDVLNVAGHRLGTAEVESALVAHHLVAEAAVVGCPHDIKGQGIYAYVTLVVDA
                     TPTEPLRKELREWVRREIGPIATPDFIQWAPGLPKTRSGKIMRRILRKIAANEHDQLG
                     DISTLADPSVVAQLVAERMIR"
     gene            complement(2900..3250)
                     /locus_tag="PL152_02280"
     CDS             complement(2900..3250)
                     /locus_tag="PL152_02280"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HQR48132.1"
                     /translation="MLAAEARIGAPYRYGGSGPDAFDCSGLVSYAYASAGITLPRTAA
                     AQFAAATPVQRGDLEPGDLVFFRVGGRDVSHVGIYAGNDEFVDAPQTGGTVRMARLDD
                     EWYSQRYVGAGRPY"
     gene            3568..4695
                     /locus_tag="PL152_02285"
     CDS             3568..4695
                     /locus_tag="PL152_02285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR48133.1"
                     /translation="MKWRAAAIAVGVIGIGVAAMAWFGRPKPVEVETVEVTRGKVDQT
                     VTNTRAGTVKACQRAKLAPPMGGQIARLPVKKGDHVERGQVLLELWNDDIRAELQLAR
                     KDAAATRALRDQACISAKVARRESDRLASLVERKLVSVELADRAAGEAEAGEAACRAA
                     GEQVLVSQARIDAASARLERTLLRAPFAGIVAEINGEVGEFVTPSPVGIATPPAVDVV
                     DSSCIYITAPIDEVDAPRVRPGMPARVTLDAFKDRSFRAHVRRVAPYVLELEKQARTV
                     EVEAEIEDRDDALLLPGYSADLEVILAERPDVLRVPTRALIEGNRVFVLEGGRVHERK
                     VTTGIGNWEYTEVTQGLAAGDRVVVSIDREGLKDGAEAVAK"
     gene            4708..5397
                     /locus_tag="PL152_02290"
     CDS             4708..5397
                     /locus_tag="PL152_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017953234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR48134.1"
                     /translation="MIELRDLSRTFQVGEQLVRALDSVSIRIGDGEYVSIMGPSGSGK
                     STLLNVIGLLDRPTAGQYLLDGRETTSLDDDELAHVRARKVGFIFQSFHLVPRLTAFE
                     NVELPMVLAAVAPDERRRRAEAVLGSLNLTDRAHHRPDQLSGGQRQRVAIARAMVMEP
                     TVLLADEPTGNLDHVSGADVLTALESLNTRGITLLVVTHDDEVAARARRHIRMRDGRV
                     VHDESAAGPSA"
     gene            5394..6605
                     /locus_tag="PL152_02295"
     CDS             5394..6605
                     /locus_tag="PL152_02295"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR48135.1"
                     /translation="MRYRDLLHLATESIVRARLRSAMMLIAMAIGVAAVVVLTSLGEG
                     ARRYVTGEFQSLGTDLLIVLPGRSETTGVQPGLFAGETPRDLTLDDARSLTRLPDVER
                     VAPMVIGSVPVSAGERSREVPVVGTNAEFYMVRRLSMSQGVFLPHGEIDRDLAVCVIG
                     RKVSSELFPHAPAVGSTLRIGERRFRVIGVLGESGRSIGFDMQELVIIPVASAEQLFN
                     TQSLFRILIQARDRDAMSRVKDAVRAELAKRHQGEEDVTVVTQDAVLATFNRIFTALT
                     LTLAGIASVSLAVAGILIMNVMLVAVSQRTAEVGLMKALGARGRQISALFLAEAVLLS
                     AAGALCGLAFGYTANWIIGRVYPALPLAPPAWAVAMAVIVAVASGVVFGLQPARKAAR
                     LDPVVALAGRH"
     gene            6609..7808
                     /locus_tag="PL152_02300"
     CDS             6609..7808
                     /locus_tag="PL152_02300"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR48136.1"
                     /translation="MRGADLVLFTSRSVRAHRMRALLTALGIAVGVAAVVLLTSIGEG
                     VRNFVVSEFTQFGTNIISVTPGRTQTMGGSIGVFGTVRPVTIDDAEALRHVPYVLASD
                     PMVQGNSEVEANGRKRRVTVYGTGPKFAEALRFKIGQGIFLPPDDPRAPRNFAVLGSK
                     MAHELFGAANPLGETVRVGSGRYRIVGVMESKGTVIGMDLDDTVFIPAARALELFDRE
                     SLFEIHVLYEENAPVAEVVDGIRRVMDARHGDEDYTVTPQQQMIDALGSVLGVLTFAV
                     AALGSISLLVGAVGIFTIMTIAVRERTSEIGLLRALGAERDDVLALFLVEAVVLSLLG
                     GLAGLLVGAGGAWLLGETVRQLPVRFSPPFIGLALGLSVVIGLVAGILPAMNAARLDP
                     VEALRAE"
     gene            complement(7825..10395)
                     /gene="hrpB"
                     /locus_tag="PL152_02305"
     CDS             complement(7825..10395)
                     /gene="hrpB"
                     /locus_tag="PL152_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HQR48137.1"
                     /translation="MITLPSLPIVDALPALRDALATHRNVLLEAPPGAGKSTVVPLAL
                     LDEPWRGHGRVVMLEPRRIAARAVAQRMAALLGEQAGQTVGYRTRLETRVGPGTRIEV
                     VTEGILTRMLQRDPALESVACVVFDEFHERNLQADLGLALAIESQRHLRDSLRLLVMS
                     ATLDGDAVLRVLDDAVAVRAPGRMYDVETRYVPPPRAAAARAPRIEATVAAVVIRALR
                     ESPGDALVFLPGAAEIRRVLEALQGGLSEADYCVLPLYGDLPAAAQDAALQADPRGRR
                     KVIVATNIAETSLTIDGVRIVVDAGLERRQRFDPATGMSRLDTLRISRASADQRRGRA
                     GRTAPGACYRLWSESAQEALLPQSPAEMLEADLAPLALELACWGCDDPASLAWLDPPP
                     AATYAQARELLQHLEALDARGRVTTLGRGMAGLGVHPRLAHMVLRARPLGFVDLACNL
                     AALLTERDPLRAAPGMLDPDVYHRVEVLEGRRPPPGMEVDAATVRRIERIARQVRQQI
                     DRNGEYAGGTSAATDAPALSTQDATGLLLAYAYPDRIGRAREGGGGRYVLSGGRGAAF
                     ATATALARSEFIVVASLDAGDREARIQLAASLDPPILERYFAGAIEETSGVHWDTRAE
                     AVAARRIRRIGALVLSDEPLGNVDPSESLAAMLDGIRSLGLGCLPWTRELEQWRSRVA
                     LVREHDPRGREGWPDVSDDALLRSLESWLAPWLGGITRREQLGRIDLRGALHGLLDWN
                     SQRRLDELAPTHLVVPSGSRLPLDYTGGTPSLAVRLQEVFGLMESPSIVLGRVPVTLE
                     LLSPARRPVQVTRDLASFWARGYHEVKKELKGRYPKHYWPDDPHDAIATRRVRPRDGN
                     "
     gene            10463..10978
                     /locus_tag="PL152_02310"
     CDS             10463..10978
                     /locus_tag="PL152_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HQR48138.1"
                     /translation="MSPFSDEAGPMTGATWMWVAATLLVAMGIAGSVLPALPGVPLVY
                     AGLVVAAWAEDFQRVTWLTLVLLGVLTLASFAIDLAATALGAKRVGATRLAIIGAIVG
                     TFAGVFLGVPGLILGPFVGAVAGELASHGRMRQAARAGLATWVGLVFGTLARIALVFT
                     MLGVFAFAYFV"
     gene            complement(10966..11829)
                     /locus_tag="PL152_02315"
     CDS             complement(10966..11829)
                     /locus_tag="PL152_02315"
                     /inference="COORDINATES: protein motif:HMM:NF026066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQR48139.1"
                     /translation="MYSDRLREAADLLEAQRANPFRVSAYRRAADTVRDLPEDVGAII
                     ERSGSEGLDELPGVGRGIASALVEMWRTGRWAQLERLRGGADPVQLFTLLPGLGHRLA
                     ERIHDELHVDSLEALEVAAHDGRLENVPGVGPRRAAAIRASLQGMLSRGRPRRAASIA
                     ATSRAAALPGKAPEPSIAALLAVDREYREKAAVNTLPMIAPRRFNPSGAAWLPVLHVT
                     RDGWHYTALFSNTALAHQLNRTRDWVVLYFYDDDHVEGQHTIVTETHGPLTGRRVVRG
                     RESECRAHYTK"
     gene            complement(11899..12339)
                     /locus_tag="PL152_02320"
     CDS             complement(11899..12339)
                     /locus_tag="PL152_02320"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQR48140.1"
                     /translation="MSRHRNPQILAYMTPFPYSVDVDAPLAEAHAFLRERHIRHLPVT
                     ERGQLVGVLTDRDIKLALGPDLGSPPERELTVRDVFQPDSYVVDAGASLLTVAATMAE
                     RHLGSALVTRDGKLVGIFTTTDACRALARVLHEQFPDQGPGEAA"
     gene            12473..>12674
                     /locus_tag="PL152_02325"
     CDS             12473..>12674
                     /locus_tag="PL152_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48141.1"
                     /translation="MLMSVMRFLRSILASVLVGIAVIGFGAYAWFLTHGPEPRVWHTA
                     HLDQEFTAAKAGEVTTIAAYREL"
     assembly_gap    12675..12774
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12775..>14072
                     /locus_tag="PL152_02330"
     CDS             <12775..>14072
                     /locus_tag="PL152_02330"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR48142.1"
                     /translation="FTAAKAGEVTTIAAYRELEDRLYAELEAKVLDKVQPADRGPFNR
                     FSTGSRSDPGKWPTNWNRTFEITPPNPVGAALLLHGLTDSPYSMRSIGQHLAGRGLKV
                     VGLRLPGHGTAPSGLLSFEIEDMQAAVRMAMRDLRRQMGPDKPIYIVGYSNGAALAVD
                     YTVQILEGADQPMPKGLVLLSPAIGISPFAVIARMRTGLSSVPGFEKAAWQVVETEFD
                     PYKYSSFSFHAAGETQRLTSGLAERVKKLAEHGPIKDFPPVLAFLSTVDATIVAPAVV
                     HALFEHLAPGSHELVLFDVNRLSGLQALLVADPGPLTQQLLAMPDRSFELTVITNATP
                     DTLQVRELQAAPGSSAQTSRLLDDSWPQHVFSLSHVAMPFPPDDSLYGYEATPDRGHV
                     QLGRVQLHGENGVLTVPDWVLTRLRSNPFHAYMLRRIDGF"
     assembly_gap    14073..14172
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14173..15240
                     /locus_tag="PL152_02335"
     CDS             <14173..15240
                     /locus_tag="PL152_02335"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQR48143.1"
                     /translation="LNRRMHNKAWIADNRIAIVGGRNVGDEYFGASDQSNFSDTDVVL
                     AGPVVEDVSREFDAYWNSPYAVPVSRFETREPKPEELEQLRRASREFTKEAGETPYIA
                     ALRDPRRRGELLAAAPPLIEVHDIRVLADDPSKIGSDEVGLAASKVLQGLAEVIQQSG
                     RQVLIVSPYFVPGKQGAEDLERGARRGIDVEVLTNSLAATDVAAVHVGYARYRKELLK
                     SGVQIYEMKRITADSQEAKHLSLLGSTSATLHTKALVVDDRWSFVGSMNLDPRSANLN
                     TEMGVLVDSPELARQLSEQFRENTASELSYHVVFDPERGLVWHDQVNGQDRELEHEPD
                     TSIGRRMTVTLLRLLPMESQL"
     gene            complement(15263..16129)
                     /locus_tag="PL152_02340"
     CDS             complement(15263..16129)
                     /locus_tag="PL152_02340"
                     /inference="COORDINATES: protein motif:HMM:NF012485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48144.1"
                     /translation="MTESVPAAHPRRLCPLRPLAFLAVLLGCTAVSAQDMQVIDLHYR
                     LADQVIPVLQPLLDPGDGLTGVDDKLFLRAGPATQARVREALAVIDRPPRQLVISVGQ
                     GTASGLSAADVRGSATVSSGDVSIGVNRPPGVDSSASVVARERQQQANLQNVSTVRAL
                     EGTEAYISMGRSVPYTTTTVTSGWGAPVVQQSTTFRDANTGFYATPRVNGDTVTLVIS
                     PRQQSYEPGRGGTIRTAGTDSTVTARLGEWVHLGAVTESTASDTGGLLVWGRYSSGSR
                     YDAWIKVDELPQ"
     gene            16157..16735
                     /locus_tag="PL152_02345"
     CDS             16157..16735
                     /locus_tag="PL152_02345"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR48145.1"
                     /translation="MIVLETARLRLRRFTKDDVDHLVTLDSDPEVMRYVTYGAPTPRS
                     TYEDVILPRWFQIYEQTPLLGYWAAESTADGSFVGWFHLRPDRFDADEQELGYRFARS
                     AWGRGYATEGSRAVIAHGFTRVGARRICARTLAANLRSRRVMEKCGLEFEQDFVYPED
                     VLQGRDESERAAVKYSIGFDAWVARSKAPSPQ"
     gene            16759..>17155
                     /locus_tag="PL152_02350"
     CDS             16759..>17155
                     /locus_tag="PL152_02350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HQR48146.1"
                     /translation="MSAGQGSPAKAIFYALLANLGIAVTKTGAAVYTNSGSMVAEAIH
                     SCADCTNQVLLFLGLRQADRPPTPEHPLGFGKASYFWSFVVALLLFSLGGLFSIYEGL
                     HKLREPEPLHQAWIALTVLGLAIALETGSL"
     assembly_gap    17156..17255
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(17300..18274)
                     /gene="hutG"
                     /locus_tag="PL152_02355"
     CDS             complement(17300..18274)
                     /gene="hutG"
                     /locus_tag="PL152_02355"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050415 - formimidoylglutamase activity
                     [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HQR48147.1"
                     /translation="MTHTRDMGAWAGRVDAADGEAGRRWHQVIRPASDDGQPGVALAG
                     FACDAGVRRNHGRPGSADGPRELRRMLGNLAWHGGNDRRLYDAGDVGCEADALEAAQQ
                     EYAALVAGLLRRGHLPVGMGGGHEIAWAAYLGLERALAGDPRLDRLGIVNIDAHLDLR
                     RPETPGRGTSGTPFLQIAEARAAVGREFRYLCAGASPAANTPALFDRASALGAEVILD
                     TEVAAPGSEERIRRFVADSSAIYLTFCLDALPASVAPGVSAPAAFGIPLGRAIALVRG
                     VLAACAHGSRPGKLLLADVAELNPQHDESGRTARTAARIVYEMASISR"
     gene            complement(18283..19566)
                     /gene="hutI"
                     /locus_tag="PL152_02360"
     CDS             complement(18283..19566)
                     /gene="hutI"
                     /locus_tag="PL152_02360"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HQR48148.1"
                     /translation="MLTLWRNARVATCNEHGQVHAAGAIVTRGGTIEWVGDAREVPSG
                     LRPDRTVELANRWVTPGLVDCHTHLVFAGQRAAEFARRTSGSSYAEIAREGGGILSTV
                     RATRAASEDDLVEQSRPRLASLLAEGVTTVEIKSGYGLDFEGESKMLRAARRLESEHP
                     VTVSTSLLAAHALPPEFSGRADDYIDTVCNDWLPRLLREGGVAAAGPAKGQSPAPRKG
                     QSPGRLVDAVDAYCEDIAFSTEQCGRLFAAARAHGLPVRLHSEQLSNIGGSQVAARYG
                     ALSCDHLEYANEADAAALARSGTVAVLLPVAFYALAEEQLPPVAALRANRVPIAVASD
                     CNPGTSPCASLLLAMNMARRLFGLTSEEVLLGATRHAARALGLEAERGSIAPGHAADF
                     AAWSVDSPDELGYWAGFNPCSMVVKDGEIVLERAV"
     gene            complement(19566..>19903)
                     /locus_tag="PL152_02365"
     CDS             complement(19566..>19903)
                     /locus_tag="PL152_02365"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649648.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HQR48149.1"
                     /translation="DSGSVASPNRETEAMRDGSDAVADWPLLNALLNTASGATWVSIH
                     HGGGVGIGYSQHAGVVIVCDGTPAAAKRIERVLWNDPASGVMRHADAGYDIAVDCARE
                     HHLNLPFLD"
BASE COUNT         2913 a         6729 c         7029 g         2932 t
ORIGIN      
        1 ccggcacgac ggccgccggg cgcaaggtga tcacggattt cctggcctcg cagggcatct
       61 ccctgccgga cttggtgctg gacaacggct cgggcctttc acgggaggag cggatcactg
      121 ccgccggcct cgccgacgtg ctgctggcgg cctggcgcag ccagtacatg cccgagttcg
      181 cggcctcgct gccgctgtcg gccacggacg ggaccctgcg caggcggttc cgctcgcctg
      241 aaatgcaggg tcggctgcgc atgaagaccg gcacgctcga cggggtgagc gcgctggccg
      301 gctacgtcaa tgcggcctcc gggcggacgt tcgtggccgt catcatcgtc aatcacccgg
      361 cggccaacgg gggtggcggc ctggcggtgc aggcggccct cgtgcagtgg ctgttcggcc
      421 agtagccccg gggcggccac cggggcgggg gaggtcatgg cccgcccgcg ctcgctatac
      481 tcgggacgac caggcgttac ggcggaccga catggcttac gacagcggca acgtgttcgc
      541 gcgcatcctg cgcggcgaga tcccggccca caccgtgtac gaggacgcgc agacgctggc
      601 gttcatggac gtcatgccgc agtcggacgg gcacacgctg gtgattccga agacgcaggc
      661 cgagaatctc ttcgacctgc cggcggacgc actggtggcg acgatcctca cgacacagcg
      721 cgtggcgcgc gcggtcaaga aggcgttcga cgcaccgggc atgatgatcg cgcagctgaa
      781 tggccgcggc gccggccaga gcgtgttcca catccatttc cacgtcgtgc cgcgttacca
      841 cggcatcgac ctcaagttcc acgcgcgcga gatggccgac ccgaagctgc tcgcggagca
      901 cgcggcgaag gtccgcgccg cgctcgactg agcgccgcgc gcggcaggtt gcgaggggga
      961 cgacgatgac gaagctccac gaggtgccgg ccggattcgc ggcccgggcg cgctaccggc
     1021 gtgacgatta cgagcggctg tatgccgagt cggtccgcga ccccgagggg ttctggggcc
     1081 gcatgggcca gcggctagcc tggatgcgac cgtacacgcg cgtccgggac gtgaacttcg
     1141 acgccaatgc cttccgcatc cgctggttcg aggacgggcg gctcaacgtg tcggccaatt
     1201 gcctcgatcg ccatctcgcg gcgcacggcg acaaggcggc gatcgtgtgg gagggcgacg
     1261 accctgccga gtccgaaacc atcacctatc gcgagctcta ctaccgcgtc tgcaagctcg
     1321 cgaacgccct gcgccagctc ggcgtgcaga agggcgaccg cgtcaccatc tacctgccga
     1381 tgattccgga agccgcggtg gcgatgctcg cctgtgcccg catcggcgcg gtccattcgg
     1441 tggtgttcgg cggattctcg ccggactcgc tcgcagggcg catcgccgac tgcgcgtcga
     1501 ccgtcgtgat cacggccgac gagggcatcc gtggcggcaa gcgcattccg ctgaaggcga
     1561 acgtggacca ggcgctgacg gcacccggca ccgagaccgt caagcacgtg ctggtggtga
     1621 agcgtactgc gtccccggtc gcgatgtacc agcgcgaccg ctggtaccac gaactggtgg
     1681 acggccagcc cgagcagtgc gagccggtgt cggtcggcgc cgaggacccg ttgttcatcc
     1741 tgtacacgtc gggatcgacc ggcaagccga agggcgtgct gcacaccacc ggcggctacc
     1801 tcgtctacgt gtcgttgacc cacgaggcgg tgttcgacct tcacgaggac gacatcttct
     1861 ggtgcaccgc cgacgtcggc tgggtgaccg gccacagcta cgtcgtgtac gggccgctcg
     1921 ccaacggcgc gacgaccgtg atgttcgagg gtgtgccgaa ctacccggac gcgagccggt
     1981 tctggcaggt gtgcgacaag cattcggtca cgatcttcta caccgcgccg accgcgttgc
     2041 gctcgctgat gcgggagggc gaggcgccgg tgaagaagtg ctcgcgcaag tcgctgcggc
     2101 tgctcggtac cgtcggcgag ccgatcaacc cggaagcctg ggagtggtat caccgcgtgg
     2161 tcggcgacgg tcgctgcccg atcgtcgaca cctggtggca gacggagacc ggcggcgtgc
     2221 tcatcacgcc gctgccgggt gcgatcgccc agaagccggg ctccgcgacg ctgccgttct
     2281 tcggcgtgca gccggccatc gtggacaacg aaggccgcgt gctcgagggg gcgtgcgaag
     2341 gcaacctcgt gatcacggac agctggccgg ggcagatgcg cacggtgttc ggcgatcacc
     2401 agcgctacgt cgacacgtac ttcaaggctt tcccgggcag ctatttcacc ggggacggcg
     2461 cacgacgcga cgaggacggt tactactgga tcaccggccg cgtcgacgac gtgctgaacg
     2521 tcgccggaca ccgcctcggc accgcggaag tcgagagcgc gctcgtcgcc caccacctgg
     2581 tggccgaggc ggcggtcgtg ggctgcccgc acgacatcaa ggggcaggga atctacgcgt
     2641 acgtcacgct ggtcgtggat gcgacgccga ccgagccgct gcgcaaggaa ctgcgcgagt
     2701 gggtgcggcg cgagatcggg ccgatcgcca cgccggattt catccagtgg gcgccgggcc
     2761 tgccgaagac tcgctccggc aagatcatgc ggcgcatcct gcgcaagatc gccgcgaacg
     2821 agcacgacca gctcggcgac atctcgacgc tggcggaccc gtcggtggtc gcgcaactgg
     2881 tcgcggaacg gatgatccgc tagtagggcc ggccggcccc gacgtagcgc tggctgtacc
     2941 actcgtcgtc gaggcgagcc atgcggaccg ttccgcccgt ctgcggcgcg tccacgaatt
     3001 cgtcattgcc ggcgtagatc ccgacgtgac tcacgtcgcg cccgcccacg cggaagaaca
     3061 ccaggtcgcc tggctcgagg tcgccgcgtt gcaccggggt cgcggccgcg aattgtgcgg
     3121 cggcagtgcg cggcagcgtg atcccggcgc tggcgtacgc ataggagacc agcccgctgc
     3181 agtcgaacgc gtcgggaccg gatccgccat agcgataggg agcgccgata cgcgcttcgg
     3241 cggcgagcaa tgcggttgtc cccgggcttg ggcgtcctgt gcttcccgca tcacccggtg
     3301 caccacccgg agccccaccc ggcgcgccgc gctggaacgg cgtgcacgct gccagcatca
     3361 acgaaatcag cagccccgcc agcggccttg cgagggcgga cggtgcggac gacggcttgc
     3421 ggtcgcgcga catggccgga gtctagtgcc gcaggatccg ggaaaacacc cactgcgtcg
     3481 caggcggcgc tgccgcgagc cctgattgcg cctccacagg cgaggtgctg caatgcaacg
     3541 gccgtacggg caacgggaat gtcgcacatg aagtggcgcg cagcggctat tgcggtcgga
     3601 gtgatcggca tcggtgtcgc tgccatggcc tggttcgggc ggccgaagcc cgtcgaagtc
     3661 gagaccgtcg aggtcacgcg cggcaaggtg gaccagacgg tcaccaacac cagggccggg
     3721 accgtcaagg cctgccagcg cgccaagctg gcgccgccga tgggcgggca gattgcgcgc
     3781 ctgccggtga agaagggcga ccacgtcgag cgggggcagg tgctgctcga attgtggaac
     3841 gacgacatcc gcgccgagct gcagctcgcg cgcaaggacg ccgccgccac ccgcgcgctg
     3901 cgcgaccagg cctgcatctc ggcgaaggtc gcgaggcgcg agtcggaccg gctcgcgagc
     3961 ctggtcgagc gcaagctcgt ctcggtggaa ctcgccgacc gcgccgcagg cgaggcggaa
     4021 gcgggcgaag ccgcctgccg ggcggcgggc gagcaggtgc tggtcagcca ggccaggatc
     4081 gacgcggcga gcgcgcggct cgagcgcacg ctgctgcgcg cgccattcgc cgggatcgtc
     4141 gccgagatca acggcgaggt gggcgaattc gtgacaccgt cgccggtcgg catcgcgacg
     4201 ccgcccgccg tcgacgtcgt ggactcgagc tgcatctaca tcacagcgcc gatcgacgag
     4261 gtggacgcgc cgcgcgtgcg gcccggcatg ccggcgcgcg tgacgctcga tgcgttcaag
     4321 gaccgcagtt tccgtgccca cgtgcggcga gtcgcgccct acgtgctcga gctcgagaag
     4381 caggcgcgca ccgtcgaggt cgaagccgag atcgaggatc gcgacgacgc gctgctgctg
     4441 cccggctaca gcgcggacct cgaggtgatc ctcgccgagc gcccggacgt gctgcgcgtg
     4501 cccacgcggg cgctcatcga gggcaatcgc gtgttcgtgc tcgaaggcgg gcgcgtccac
     4561 gagcgcaagg tgacgacggg catcggcaac tgggaataca ccgaggtcac gcagggcctc
     4621 gccgccggcg atcgcgtcgt cgtgtcgatc gaccgcgagg ggctgaagga cggcgccgag
     4681 gcggtggcga aataggcgcg cgccgccatg atcgaactca gggacttgag ccggaccttc
     4741 caggtgggcg agcagctggt gcgcgccctc gactcggtct cgatccgcat cggcgacggc
     4801 gagtacgtgt cgatcatggg gccgagcggg tcgggcaagt ccacgctgct gaacgtgatc
     4861 ggactgctgg accggccgac ggccgggcag tacctgctcg acggtcgcga gacgacgtcg
     4921 ctcgacgacg acgaacttgc gcacgtccgc gcccgcaagg tcggcttcat cttccagtcg
     4981 ttccatctcg tgccgcgcct gacggccttc gaaaacgtgg agctgccgat ggtgctggcc
     5041 gcagtggcgc ccgacgagcg ccggcgccgc gcagaggcgg tgctcgggag cctgaacctc
     5101 acggaccgtg cgcaccaccg gccggaccag ctgtccggcg gccagcgaca gcgcgtggcg
     5161 atcgcgcgcg cgatggtgat ggaaccgacc gtgctgctgg ccgacgagcc caccggcaac
     5221 ctcgaccacg tctcgggtgc cgacgtcctg acggcgctcg agtcgctcaa cacccgcggg
     5281 atcaccctgc tggtggtgac gcacgacgac gaggtcgcgg ccagggctcg ccggcacatc
     5341 cgcatgcgcg acgggcgcgt cgtccacgat gaatccgccg caggaccgag tgcatgagat
     5401 accgggatct gctgcacctc gcgaccgagt cgatcgtccg tgcgcgactg cgcagcgcga
     5461 tgatgctgat cgcgatggcg atcggcgtgg ccgcggtcgt cgtgctgacc tcgctgggcg
     5521 agggcgcgcg tcgctacgtc accggcgagt tccagtcgct cggcacggac ctgctgatcg
     5581 tcctgccggg gcggtcggag acgaccgggg tgcagccggg gctcttcgcg ggcgagactc
     5641 cgcgcgacct gacgctggac gacgctcggt ccctcacgcg cctcccggac gtcgagcgcg
     5701 tcgcgcccat ggtcatcggc tcggtcccgg tatccgcggg ggagcggtcg cgtgaagtgc
     5761 ctgtcgtcgg caccaacgcg gagttctaca tggtccggcg gctgagcatg tcgcagggcg
     5821 tgttcctgcc gcacggcgaa atcgaccgtg acctcgcggt gtgcgtgatc ggccgcaagg
     5881 tgagttccga gctgttcccg cacgcgccgg cggtgggttc gacgctcagg atcggcgagc
     5941 gtcgctttcg cgtgatcggc gtgctggggg aatcggggcg gtcgatcggc ttcgacatgc
     6001 aggagctggt gatcatcccg gtggcgagcg ccgagcagct gttcaacacg cagtcgctgt
     6061 tccgcatcct gatccaggcg cgcgacaggg acgcgatgtc gcgcgtgaag gacgctgtgc
     6121 gcgcggagct ggcgaagcgc caccagggcg aggaggacgt cacggtcgtc acgcaggatg
     6181 ccgtgctcgc gacgttcaat cgcatcttca ccgcgctgac gctgacgctg gccggcatcg
     6241 catcggtgag cctcgcggtc gccggcatcc tcatcatgaa cgtgatgctg gtcgcggtga
     6301 gccagcgcac ggccgaagtc ggcctgatga aggcgctcgg agcgcgcgga aggcagatca
     6361 gcgcgctgtt cctcgcggag gcggtgctgc tgtcagcagc gggcgcgctg tgtggtcttg
     6421 cgttcgggta taccgcaaac tggatcatcg gccgcgtgta ccccgcgctt ccgctggcgc
     6481 ctccggcctg ggccgttgcc atggcggtca tcgtcgctgt cgcaagcggc gtggtgttcg
     6541 gcctgcagcc ggcacgcaag gccgcgcgcc tcgatccggt cgtcgcgctg gccgggcggc
     6601 actgagcgat gcgcggcgcc gacctcgtgc tgttcacctc gcgctcggtc cgtgcgcacc
     6661 gcatgcgggc gctgctgacc gcgctcggta tcgcggtcgg cgtcgccgcg gtcgtgctgc
     6721 tcacctcgat cggggagggc gtgcggaact tcgtggtctc cgagttcacg cagttcggca
     6781 cgaacatcat cagcgttacc ccgggaagga cccagacgat gggtggctcc atcggggtgt
     6841 tcggcaccgt gcggccggtg accatcgacg atgcagaggc gttgcggcac gtgccgtacg
     6901 tgctcgcttc ggacccgatg gtgcagggca actccgaggt cgaggccaac ggccgcaagc
     6961 gtcgcgtcac cgtgtacggc accgggccga aattcgcgga ggcgctgcgc ttcaagatcg
     7021 gccagggcat cttcctgccg ccggacgacc cgcgcgcgcc gcgcaacttc gcggtcctgg
     7081 gatcgaagat ggcgcacgag ctcttcggag cggcgaaccc gctcggcgag accgtgcgcg
     7141 tcggcagcgg gcgctaccgc atcgtcggcg tgatggagtc gaagggcacg gtcatcggca
     7201 tggacctcga cgataccgtc tttatccccg ccgctcgtgc gctcgagctg ttcgaccgcg
     7261 agagcctgtt cgagatccac gtgctgtacg aggagaatgc gccggtcgcg gaggtcgtgg
     7321 acgggatacg ccgcgtgatg gacgcgcggc acggcgacga ggactacacg gtcaccccgc
     7381 agcagcagat gatcgacgcg ctgggctcgg tgctcggcgt gctgacgttc gcggtcgccg
     7441 cgctcggcag catctcgctg ctggtcggcg cggtcgggat cttcacgatc atgaccatcg
     7501 cggtgcggga acggaccagc gagatcgggc tgctgcgggc gctcggggcg gaacgcgacg
     7561 acgtgctcgc gctgttccta gtcgaagcgg tcgtgctctc gctgctcggt ggcctggccg
     7621 gtctgctggt cggcgcaggc ggtgcctggc tgctcggcga aacggtccgg cagctgcccg
     7681 tgcgcttctc gccgccgttc atcggcctgg cgctcgggct ctcggtcgtg atcgggctcg
     7741 tggcagggat cctgccggcg atgaacgccg cgaggctcga tccagtggag gcgctgcgag
     7801 cggagtagcg gtccgccggt gctctcaatt cccgtcgcgc ggtcgcacgc gccgggtcgc
     7861 aatggcgtcg tgcggatcgt cgggccagta gtgcttcggg tagcggccct tcagttcctt
     7921 cttcacctcg tggtaaccgc gtgcccagaa gctcgcgagg tcgcgggtca cctgcacggg
     7981 acgccgcgcc ggcgagagca gctcgagcgt caccgggacg cggcccagaa caatgctcgg
     8041 cgactccatg agcccgaaga cttcctgcag ccgcacggca agcgacggcg tgccgccggt
     8101 gtagtccagc gggagcctcg agccgctcgg aacgacgagg tgcgtcggcg ccagctcgtc
     8161 gaggcgccgc tgggagttcc agtcgagcag tccatgcagc gccccgcgca ggtcgatcct
     8221 gccgagctgc tcacgccggg tgattccacc gagccacggt gccagccagg actcgagtga
     8281 gcggaggagc gcgtcgtcgg agacgtccgg ccagccttca cggccgcgcg ggtcgtgctc
     8341 gcgaacgagg gctacccgtg agcgccactg ctccagttcg cgggtccacg gcaggcagcc
     8401 gaggccgagc gatcgtatcc cgtcgagcat cgccgccagg gactccgatg gatcgacgtt
     8461 gcccagcggc tcgtcgctca gcaccagtgc gccgatccgg cggatgcgcc tggccgcgac
     8521 tgcctccgcc cgcgtgtccc agtgaacgcc cgacgtctcc tcgatggcgc ctgcaaagta
     8581 tcgttcgaga attggcgggt cgagtgaagc tgccagctgg atgcgcgcct cgcggtcgcc
     8641 ggcgtcgagc gacgcgacca cgatgaactc ggaccgcgcc agtgccgtcg cggtcgcgaa
     8701 tgccgctccc ctgccgccgc tcagcacgta gcgaccgccg ccgccttcac gagcgcggcc
     8761 gatccggtcc ggatacgcgt aggcgagcag caggccggtc gcgtcctgcg ttgaaagcgc
     8821 gggcgcgtcc gtggcggcgc tggtgccacc tgcgtactca ccattgcgat cgatttgctg
     8881 ccggacctgc cgggcgattc gctcgatgcg gcggaccgtg gccgcatcga cctccatgcc
     8941 gggtggcggg cgccgaccct cgagcacttc gacgcggtga tacacgtcgg ggtcgagcat
     9001 gcccggcgcc gcgcgcagcg gatcccgctc cgtgagcagc gccgcgaggt tgcacgcgag
     9061 atcgacgaat ccgagtggcc gcgcacgcaa caccatgtgt gcgagccgcg gatgcacgcc
     9121 gagtccggcc atgccgcggc cgagcgtcgt gacgcgcccc ctggcatcga gggcctcgag
     9181 gtgctgcagc aactcgcgcg cctgggcata ggtcgcggcc ggcggcggat ccagccaggc
     9241 cagtgacgcc ggatcgtcgc agccccagca ggcgagttcg agcgccaggg gcgccaggtc
     9301 ggcctcgagc atttctgcgg gcgactgcgg cagcagcgcc tcctgcgcgg actcgctcca
     9361 caggcggtaa caggcgccgg gcgcggtgcg acctgcgcga ccacggcgct ggtccgccga
     9421 cgcacgcgag atgcgcagcg tgtcgagccg gctcatgccg gtcgcggggt cgaatcgctg
     9481 ccggcgctcg agccctgcgt cgacgacgat ccggacgccg tcgatcgtca ggctcgtctc
     9541 cgcgatgttg gtcgcgacga tcaccttgcg gcgcccgcgg ggatcggcct gcaatgccgc
     9601 gtcctgtgcc gcggcgggca ggtcgccgta cagcggcagc acgcagtagt cggcctcgga
     9661 caggccgccc tgcagtgcct cgaggacgcg ccggatctcc gcggcacccg gcaggaatac
     9721 gagcgcgtcg cccggactct cgcgcagggc gcggatcacg acggctgcca ccgtcgcttc
     9781 gattcggggc gcacgtgcgg cggctgcgcg aggcggcgga acgtatcgcg tctcgacgtc
     9841 gtacatgcgg cccggcgcgc gcacggccac tgcgtcgtcg agcacccgca ggactgcgtc
     9901 gccgtccagg gtggcggaca tgaccagcag gcgcagcgaa tcgcgcaggt gccgctggct
     9961 ctcgatcgcc agcgccaggc cgaggtcggc ctgcaggttg cgctcgtgga attcgtcgaa
    10021 cacgacgcac gccacggact cgagcgcggg gtcgcgctgc agcatgcgcg tgaggatgcc
    10081 ctcggtcacg acctcgatgc gggtgcccgg gccgacgcgc gtctcgagcc gcgtgcgata
    10141 gccgaccgtc tgtccggcct gctcgccgag cagggccgcc atgcgctgcg ccactgcgcg
    10201 cgcggcgatg cgtcgcggct cgagcatcac gacccggccg tggcctcgcc atggctcgtc
    10261 gagcagggcg agcgggacga ccgtgctctt gccggccccc ggcggcgcct cgagcagcac
    10321 gttgcgatgc gtggccagcg cgtcacgcag cgcgggcagt gcgtcgacga tcggcaggct
    10381 gggcagcgtg atcatggatc gcgcagtgta gcccggcggg aaataaggac attctcctat
    10441 ttctggcgac aaaaatagga gaatgtcccc attttcggac gaggcggggc cgatgaccgg
    10501 tgcgacgtgg atgtgggtgg cggcgacgct gctggtggcg atgggcatcg ccggatcggt
    10561 gctgcctgca ctgcccggcg tgccgctcgt ctatgccggg ctcgtggtcg ctgcctgggc
    10621 ggaagacttc cagcgcgtca cgtggctgac gctcgtgctg ctcggagtgc tcacgctggc
    10681 atcgttcgcg atcgacctcg cggcgaccgc gctcggcgcg aagcgcgtcg gcgccacgcg
    10741 cctcgcgatc atcggcgcga tcgtcggcac gttcgccggg gtgttcctgg gggttcccgg
    10801 gttgatcctc ggcccgttcg tcggcgcggt tgcgggcgaa ctcgcctcgc acggccggat
    10861 gcggcaggcg gcgcgcgccg ggctcgcgac gtgggtcggt ctcgtcttcg gcacgctggc
    10921 gcggatcgcg ctcgtgttca cgatgctcgg cgtgttcgcg ttcgcctact tcgtgtagtg
    10981 cgcgcggcac tccgactcgc ggccgcgcac gacgcggcgg cccgtgagcg gcccgtgcgt
    11041 ctcggtcacg atcgtgtgct ggccctcgac gtggtcgtcg tcgtagaagt acagcaccac
    11101 ccagtcacgg gtgcgattga gctggtgcgc aagcgcggtg ttggagaaga gcgcggtgta
    11161 gtgccagccg tcgcgcgtca cgtgcaggac cggcagccat gcggcgcccg acgggttgaa
    11221 tcgcctgggc gcgatcatgg gcagcgtatt gaccgcggcc ttctcccggt actcgcggtc
    11281 cacggcgagc agcgcagcga tggacggctc gggcgccttg ccgggcagtg ccgccgctcg
    11341 gctcgtggct gcaatcgacg cagcgcgccg cggacgcccg cgtgacagca tgccttgcag
    11401 gcttgcgcgg atggccgctg cgcggcgcgg cccgacgccc ggcacgttct cgagccgtcc
    11461 gtcgtgcgct gccacctcca gcgcctcgag gctgtcgacg tgcagctcgt cgtggatccg
    11521 ctcggccagg cggtgcccga gccccgggag cagcgtgaac agctgcaccg ggtcggcgcc
    11581 gccgcgcagg cgttccagtt gcgcccagcg cccggtccgc cacatctcga cgagtgcact
    11641 ggcgattccg cgcccgacgc ccggcaactc gtcgagcccc tccgaccccg agcgctcgat
    11701 gatcgctccg acgtcctcgg gcaggtcgcg caccgtgtcc gcagcgcggc gataggcgga
    11761 gacccggaac gggttggcgc gctgggcctc cagcaggtcg gcggcctcgc gcagtcggtc
    11821 ggaatacatg gcgttggtcc gcgccctgag cgcgcccggg ggcacggtgc ctggggaggg
    11881 ggcgctggat accatggttc aggccgcttc gccgggaccc tggtccggga actgctcgtg
    11941 caggactcgc gccagcgcgc ggcaggcgtc cgtcgtagtg aagatgccga ccagcttgcc
    12001 gtcgcgagtc accagcgccg agccgaggtg gcgctcggcc atcgtcgcgg cgaccgtcag
    12061 caacgatgcc ccggcatcga ccacgtacga gtctggctgg aacacgtcgc gcaccgtgag
    12121 ctcccgctcc ggcggggaac cgaggtccgg gccgagcgcc agcttgatgt cgcggtccgt
    12181 gagcacgccg accagctgtc cccgctccgt gaccggcagg tggcggatgt gccgctcgcg
    12241 caggaatgca tgggcctcgg ccagtggcgc gtccacgtcc acggagtacg ggaacggcgt
    12301 catgtaggcg aggatctgcg gattgcggtg gcgcgacatg gcccggtcag gaccccccaa
    12361 cgacagggcg atgctgcgcc agcggcgtgg ccgcgcacat cggggtttta cgagacgggt
    12421 cgccgttgtt ctatgatccg gcgcctcacg ccagccgaac ggtgcctcgc ccatgcttat
    12481 gtccgtgatg cgcttcctgc gctcgatcct ggcctcggtc ctggtcggga tcgcggtcat
    12541 cggcttcggg gcctatgcct ggttcctgac acacgggccc gagcccaggg tctggcacac
    12601 ggcgcacctc gaccaggaat tcaccgcggc caaggccggc gaggtgacca ccatcgcggc
    12661 ctaccgcgag ctcgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnttcacc
    12781 gcggccaagg ccggcgaggt gaccaccatc gcggcctacc gcgagctcga ggaccggctg
    12841 tacgcagagc tcgaggcgaa ggtgctggac aaggtgcagc cggccgatcg cgggccgttc
    12901 aaccgcttca gcacggggag ccgcagcgat cccggcaagt ggccgaccaa ctggaaccgc
    12961 accttcgaga tcacgccgcc caacccggtc ggcgcggcgc tgctcctgca cggactgacg
    13021 gattcgccgt acagcatgcg ctcgatcggt cagcacctcg ccgggcgcgg gctcaaggtc
    13081 gtcggcctgc ggttgccagg gcacggcacg gccccttcgg gcctgttgag cttcgagatc
    13141 gaggacatgc aggcggccgt gcggatggcg atgcgcgacc tgcgccggca gatgggcccg
    13201 gacaagccga tctacatcgt cggctattcg aacggggccg cgcttgccgt ggactacacc
    13261 gtgcagatcc tcgagggggc ggaccagccg atgccaaagg gtctcgtcct gctctcgccg
    13321 gccatcggga tttcaccgtt cgcggtgatc gcgcgcatgc gcacgggcct gtcgtccgtg
    13381 ccggggttcg agaaggcggc ctggcaggtc gtggagacgg agttcgatcc gtacaagtat
    13441 tcctcgttca gcttccacgc ggccggcgag acgcagcgac tcaccagcgg gctcgcggag
    13501 cgcgtgaaga agctcgcgga gcacggcccg atcaaggatt tcccgcccgt gctcgcgttc
    13561 ctgtccacgg tcgacgcgac gatcgtcgcg ccggcggtcg tgcacgcgct gttcgagcac
    13621 ctcgcgcccg ggtcgcacga gctcgtgttg ttcgacgtca atcgcctgtc cggcctccag
    13681 gcactgctcg ttgccgaccc cggcccgctc acgcagcagt tgctcgcgat gcctgatcgc
    13741 tccttcgagc tcacggtgat cacgaacgcg acgcccgaca ccctgcaggt ccgggaactg
    13801 caggccgctc cgggctcgtc ggcccagacc tcgaggttgc tcgacgattc ctggccccag
    13861 cacgtgttct cgctgtcgca cgtcgcgatg ccgttcccgc ccgacgactc gttgtacggg
    13921 tacgaggcga cccccgatcg cgggcacgtg caactcggcc gcgtgcagct gcacggcgag
    13981 aacggggtcc tgaccgtgcc cgactgggtg ctgacgcggc tgcgcagcaa tcccttccac
    14041 gcctacatgc tgcggcggat cgacggattc atnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnctcaaccg ccggatgcac aacaaggcgt ggatcgccga caaccgcatc
    14221 gcgatcgtcg gcggccgcaa cgtcggcgac gagtacttcg gcgcgtccga ccagtccaat
    14281 ttcagcgaca cggacgtcgt gctcgccggg ccggtggtgg aggacgtgag ccgcgagttc
    14341 gacgcatact ggaacagccc gtacgcggtg cccgtgtccc gcttcgagac gcgcgagccg
    14401 aaacccgagg aactcgagca actgcgaagg gcgtcgcggg agttcaccaa ggaagccggg
    14461 gagacgccct atatcgcggc gctgcgggat ccacggcgtc gcggggaact gctcgccgcc
    14521 gcgccgccgc tgatcgaggt gcacgacatc cgcgtgctgg cggacgaccc gtcgaagatc
    14581 ggcagtgacg aggtcgggct cgcggcatcg aaggtgctgc aggggctggc cgaggtgata
    14641 cagcagtccg gaaggcaggt cctgatcgtc tcgccgtact tcgtgcccgg caagcagggt
    14701 gccgaagacc tcgagagagg tgcgcggcgc ggcatcgacg tcgaggtatt gaccaactca
    14761 ctggcggcga ccgacgtggc ggccgtgcac gtcggttacg cgcgctaccg caaggaactg
    14821 ctgaagagcg gcgtgcagat ctacgagatg aagcgcatca ccgcggactc gcaggaagcc
    14881 aagcacctga gcctgctcgg ctcgacgagc gccacgctgc acacgaaagc cctggtcgtg
    14941 gacgatcgct ggtcgttcgt cggctcgatg aacctcgatc cgcgctccgc gaacctgaac
    15001 accgagatgg gcgtgctcgt ggacagcccc gaactcgcac gccagctgag cgagcaattc
    15061 agggagaaca ccgcgtcgga gctgagctac cacgtcgtgt tcgacccgga gcgaggcctc
    15121 gtctggcacg accaggtgaa cggccaggac cgcgaactgg agcacgagcc ggacaccagc
    15181 atcggccggc gcatgacggt caccctgctg cggctgctgc cgatggagtc gcagctctga
    15241 gcagcccggc cgccgggcgc ggctactggg gcagctcgtc caccttgatc caggcgtcgt
    15301 accgggaccc cgacgagtat cggccccaca ccagcagtcc gcccgtgtcc gacgcggtgc
    15361 tttcggtcac cgcgccgagg tgcacccact cgccgagccg cgccgtcacg gtcgaatccg
    15421 ttcccgcggt gcggatcgtg ccgccgcgcc cgggctcgta ggactgctgt cgcggcgaga
    15481 tcaccagcgt cacggtgtcc ccattcacgc gcggcgtggc gtagaaaccg gtgttcgcgt
    15541 cgcggaacgt cgtggattgc tgcacgaccg gggcgcccca gccggaggtc accgtcgtcg
    15601 tcgtgtacgg cacggagcgg cccatcgaga tgtaggcctc cgtgccctcg agtgcgcgca
    15661 cggtgctgac attctgcaag ttcgcctgct gctggcgttc tcgtgcgacc acgctggccg
    15721 aggaatcgac gcccggcggc cggttcacgc cgatcgacac gtcgcccgac gagaccgtcg
    15781 ccgagccgcg cacgtcggcg gctgacagac cgctggccgt gccctggcca acgctgatca
    15841 cgagctgccg tggaggacga tcgatgacgg cgagggcctc gcggacccgc gcctgcgtcg
    15901 ccggacccgc gcgcaggaac agcttgtcgt ccacgccggt gagaccgtcg cccggatcca
    15961 gcaggggttg cagcaccggg atgacctggt ccgccaggcg gtaatgcagg tcgatcacct
    16021 gcatgtcctg tgccgacacc gccgtgcagc ccagcaggac ggccaggaac gccagcgggc
    16081 gcagcgggca caggcgacgg ggatgcgcgg cgggcacgga ttcggtcatg atcgagcctc
    16141 gcgggaggtc gtcggcatga tcgtactaga gacggcgcgg ctgcggttgc gacgcttcac
    16201 gaaagacgac gtggatcacc tggtgacgct ggactccgac ccggaggtga tgcgctacgt
    16261 cacctacggc gcgccgaccc cccgaagcac gtatgaggac gtcatcctgc cgcgctggtt
    16321 ccagatctac gagcagacgc cgctgctcgg gtactgggcc gccgagagca ccgcggacgg
    16381 gagcttcgtc ggctggtttc acctccggcc cgaccggttc gacgcggacg agcaggaact
    16441 cggctaccga ttcgcccgct ccgcctgggg gcgaggctac gcgacggaag gctcgcgcgc
    16501 ggtgatcgcg cacggcttca cgcgggtcgg tgcgcggagg atctgcgcgc gcaccctcgc
    16561 ggccaatctc cgctcgcgtc gggtgatgga gaaatgcggc ctcgagttcg agcaggactt
    16621 cgtctacccg gaggacgtgc tgcagggacg cgacgagtcg gagcgcgcgg cggtcaagta
    16681 ctccatcggt ttcgacgcgt gggtggcgcg aagcaaggcg ccgtcgccac aatgaccacg
    16741 caacagggag cagaatcgat gtccgcgggg cagggctcgc cggccaaggc aatcttctac
    16801 gcgctcctgg ccaacctcgg catcgcggtc accaagaccg gggccgccgt ctatacgaac
    16861 tcgggcagca tggtcgcgga ggcgatccac tcctgcgcgg actgcaccaa ccaggtgctg
    16921 ctgttcctcg gcctcaggca ggccgaccgg ccgccgaccc ccgagcaccc gctcggcttc
    16981 ggcaaggcca gttacttctg gagtttcgtg gtcgcgctcc tgctcttcag cctgggcggg
    17041 ctgttctcga tctacgaagg cctgcacaag ctgcgggagc ccgaaccact ccaccaggcc
    17101 tggatcgcgc tcaccgtgct cgggctggcg atcgcgctcg agacgggcag tctcgnnnnn
    17161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggagg cccacccgga agtgggctgg
    17281 tgcttcctcg agcccgacgt cagcgactga tcgacgccat ctcgtagacg atgcgtgccg
    17341 cggtcctggc cgtgcggccg gactcgtcgt gctgcgggtt cagttccgcg acatccgcga
    17401 ggagcagctt gccgggacgc gagccgtgcg cacaggctgc gagcacgccg cgcacgagtg
    17461 cgatggcccg cccgagcggg atgccgaagg ccgccggtgc gctgacgccg ggcgcgaccg
    17521 aagccggcag cgcgtccagg cagaaggtca ggtagatcgc ggaagagtcg gcgacgaaac
    17581 ggcggatgcg ctcctcgctg ccgggcgctg cgacctcggt gtcgagtatc acttcggctc
    17641 cgagtgccga cgcccggtcg aacagcgccg gcgtgttcgc ggccgggctc gcgccggcgc
    17701 agagatagcg aaattcgcgg ccaaccgccg cgcgtgcctc ggcaatctgc aggaacggcg
    17761 tgccggacgt gccccgaccc ggtgtctccg gccggcgcag gtcgaggtga gcatcgatgt
    17821 tgacgatccc gagacggtcg aggcgtggat cgccggcgag cgcgcgctcg agccccaggt
    17881 acgcagccca tgcgatctca tggcctccgc ccatgccgac cggaaggtgc ccgcgccgca
    17941 gcaggccggc cacgagggct gcgtattcct gctgtgcggc ttcgagcgcg tctgcttcgc
    18001 agccgacatc acccgcgtcg tagagccgcc ggtcgttgcc gccgtgccag gcgaggttgc
    18061 cgagcatgcg ccgcaattct cgcgggccgt ccgcggaccc ggggcgaccg tggttgcgtc
    18121 gcacgcccgc gtcgcaggca aatcctgcaa gcgcgacccc gggttggccg tcatcgctcg
    18181 cagggcggat cacctggtgc cagcgacggc cggcctcgcc gtccgcggca tccacgcggc
    18241 cagcccatgc tcccatgtct ctggtatgcg tcatcgcacg cctcacacgg ctcgttcgag
    18301 gacgatctcg ccgtccttca cgaccatgct gcaagggttg aatccggccc agtagccgag
    18361 ctcgtccggg gagtcgacgg accaggctgc aaagtccgct gcatggccgg gtgcgatcga
    18421 gccgcgctcg gcctccaggc ccagtgcacg cgcggcatgg cgcgtcgcgc cgagcaggac
    18481 ctcctcgctc gtgagcccga acagtcgacg agccatgttc atcgcgagca gcagcgacgc
    18541 gcagggcgag gtgcccgggt tgcagtcgga cgcgaccgcg atcggcaccc ggttcgcgcg
    18601 caatgcggcg accggcggca gctgctcctc tgcgagcgcg tagaaagcca ccggcagcag
    18661 caccgcgacc gtgcccgatc gggcgagcgc cgcagcgtct gcctcgttcg cgtactcgag
    18721 gtggtcgcag gacagggccc catatcgcgc ggcgacctgg ctgccgccga tgttggaaag
    18781 ttgctcggag tgcaggcgga ccgggaggcc gtgcgcgcgc gccgccgcga acagccgccc
    18841 gcactgctcc gtcgagaacg cgatgtcctc gcagtacgcg tccaccgcgt cgaccagccg
    18901 ccccggggac tgtcccttgc ggggagcagg ggactgtccc ttggctggac cagctgccgc
    18961 gaccccgcct tcgcgcagca gacgcggcag ccagtcattg cagacggtgt cgatgtaatc
    19021 gtcggcgcga ccggagaatt ccggcggcag tgcgtgtgcg gcgagcagac tggtcgagac
    19081 ggtgaccgga tgctccgatt cgagacggcg tgcggcacgc agcatcttcg attcgccctc
    19141 gaagtcgagc ccgtagcccg acttgatctc gaccgtcgtg acgccttccg cgagcagcga
    19201 cgcgaggcgc ggccggctct gctcgacgag gtcgtcctca ctcgccgccc gggtcgcgcg
    19261 aaccgtgctc aggataccgc cgccttcgcg cgcgatctcc gcatagctcg atccggaggt
    19321 gcgtcgcgca aactcggcgg cgcgctgtcc ggcgaacacc aggtgtgtgt ggcagtcgac
    19381 gagtcccggc gtcacccaac gattcgcgag ctcgacggtg cgatccggtc gcagtcccga
    19441 cggtacctcg cgcgcgtcac ccacccactc gatcgtgcca ccgcgggtga cgatcgcgcc
    19501 cgcagcgtgc acctggccgt gctcattgca ggtcgcgaca cgcgcgttgc gccagagcgt
    19561 caacatcagt cgaggaaggg gaggttgagg tgatgctcgc gggcgcagtc caccgcgatg
    19621 tcgtagcccg cgtcggcatg gcgcatcacg ccggaggcgg ggtcgttcca cagcacgcgc
    19681 tcgatgcgct tcgcggcggc cggcgttccg tcgcagacga tcaccacgcc cgcatgctgc
    19741 gagtagccga tgccgacccc gccgccgtgg tggatcgata cccaggtggc gccgctcgcg
    19801 gtgttgagca gcgcattcag cagcggccag tcggccacgg cgtcggaccc gtcgcgcatc
    19861 gcttcggtct cgcggttcgg gctcgccacc gagccggagt cga
//
LOCUS       DAOMFD010000020        19625 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_515_length_19625_cov_37.598109, whole genome shotgun sequence.
ACCESSION   DAOMFD010000020 DAOMFD010000000
VERSION     DAOMFD010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19625)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19625)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19625
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_515_length_19625_cov_37.598109"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PL152_02370"
     CDS             <1..309
                     /locus_tag="PL152_02370"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184527.1"
                     /note="catalyzes the formation of porphobilinogen from
                     5-aminolevulinate; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQR48150.1"
                     /translation="NSDEALREVALDLEEGADMVMIKPGLPYLDIVRRVKDRFGVPTF
                     VYQVSGEYAMLMAAAGNGWLDERAVVLEALTSIKRAGADGILTYFARRAAQWLKEINP
                     "
     gene            372..1223
                     /gene="aroE"
                     /locus_tag="PL152_02375"
     CDS             372..1223
                     /gene="aroE"
                     /locus_tag="PL152_02375"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF001310.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HQR48151.1"
                     /translation="MSAAYGLFGYPVQHSWSPFIHGMFAKQTGQEMTYRLFESPPERF
                     RSEILGFLGTGGGSGANVTLPHKQAATELVNELTARAQMADAINTIVRTESGLLGDNT
                     DGAGLLTDLTRNLGLGWSAPRILVLGAGGAARGAIGPLLSLKPRMIVIANRTAARAEQ
                     LAWEFRDFGAVSGCEYSRLENQRFDLIVNATAASLRGEVPLIPIGVVDSMTTCYDMAY
                     GVGDTAFVAWAKRIGAGRAEQGWGMLVEQAAEAFELWRGVRPDTAPVLQALHKRAATG
                     PRNPSSG"
     gene            complement(1192..1722)
                     /locus_tag="PL152_02380"
     CDS             complement(1192..1722)
                     /locus_tag="PL152_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010482194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HQR48152.1"
                     /translation="MPVLPYLDLRPKLGPGTYVDPAALVIGDVETGADVSIWPMAVVR
                     GDVNQIRIGARSNVQDGSVLHVSRPYPGNDTGWPLLIGEDVVIAHKVVVHGCTIGNRV
                     LVGIGAIVLDGVVVEDDVTIGAGAVVTPGKRLESGGLYVGNPARRARELTPAEVARIP
                     IMAAWYVNLKKDYEGP"
     gene            1828..2400
                     /locus_tag="PL152_02385"
     CDS             1828..2400
                     /locus_tag="PL152_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQR48153.1"
                     /translation="MIVVLLLLVALLVVAAVYVVRIFNGLVALRENVRKAWANIDVLL
                     TQRHDELPKLVETCKRYMGYERETLERVMQARAALFQAQGRGDVAAVGAAEQQLRDGI
                     GRLFAVAENYPALKADEGFRHLQGRITELEEGIADRRELYNESVNLLNIRIQTFPDLV
                     VARLFDFKAASLLEFAEEQKRDVDLGKLFG"
     gene            2393..>3105
                     /locus_tag="PL152_02390"
     CDS             2393..>3105
                     /locus_tag="PL152_02390"
                     /inference="COORDINATES: protein motif:HMM:NF023899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004842 - ubiquitin-protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006996 - organelle organization [Evidence
                     IEA];
                     GO_process: GO:0016567 - protein ubiquitination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIDE domain-containing protein"
                     /protein_id="HQR48154.1"
                     /translation="MAEASDFWFWVIALSFAVAGGGWLSLRSLRIARLLEDTPTSRIR
                     SAAQGYVELSGRCRAMDGTRNDAPLTQRPCVWWRYRIQQKSESGAPGKRRESWTTINS
                     GRSELPFLLDDGTGECIVQPSGAEVVTGESTTWYGSTPWPTQAAGAGLLHSQEHRYRY
                     FEERVYEHEQLCLLGKFQSLTSTTASDSQAEVAALLADWKDDQPALVERFDRDRDGRV
                     SLAEWEQAREAARRTVAERA"
     assembly_gap    3106..3205
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3206..5209
                     /locus_tag="PL152_02395"
     CDS             3206..5209
                     /locus_tag="PL152_02395"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQR48155.1"
                     /translation="MQASQLPRFSAFDPDRVEPAIRQLLDDNRREIAALLDDGASGWD
                     GLVAPLERMQHALACAWSPVGHLNGVMNGDRIRAAYNACLPLLTAYHTELAQNARLCA
                     AYQRVYDTEGARLDEAQRTLLEHALRDFRLSGVSLPDDRKRRFGEVMERLATVQAKFD
                     ENVLDATNAWSRHIDLESQLDGLPPTTVERARRAAAESGRPGWLLALDAPNYQAVMTH
                     ASDAELRREFYEAWTTRASDQGPHAGRWDNTGLMAEILGLRHEAANLVGFADYAQYSL
                     ATKMARETGEVLSFLRELARLSRPAAEREFADLERYSGRKLEAWDVAYWSERLKHERF
                     EVSAESLRPYFPLPRVLAGLFTVVSRLYRIRIVARAGADVYHPDVRYFDVLDRDGSPR
                     GGFFVDLYARPKKRGGAWMDDCVGRMRIGPSSALPVAYLVCNFTPPVGDRPSLLTHDE
                     VLTLFHEFGHGLHHMLTRVDYPSVAGINGVPWDAVELPSQFMENFAWRHEVLPLISAH
                     VETGEPLPSATLAKLQATRTFQAGMQTVRQLEFALFDFRLHAEHVPGAPPRIAETLEE
                     VRREVAVVRPPAFNRFPHSFQHIFSGGYAAGYYSYKWAEVLSADAFGAFEEHGVFDRA
                     TAASFLRSILERGGSRDAMEAFVEFRGRKPRLEPLLRQMGLAA"
     gene            5212..5979
                     /gene="xth"
                     /locus_tag="PL152_02400"
     CDS             5212..5979
                     /gene="xth"
                     /locus_tag="PL152_02400"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008853 - exodeoxyribonuclease III
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HQR48156.1"
                     /translation="MLLATWNVNSLRVRLPHLRDWLAANPLDVLALQETKIPDADFPR
                     GEIESLGMRAVFSGQRTYNGVAILSKEEPRDVVAGIPAFDDEQRRVIAATVGGVRVID
                     VYVPNGQEVGSEKFEYKLRWLEALRGYVVAELARHPRLVVLGDYNIAPEDRDVHDPKA
                     WEGSVHVSEPERAALRALVAAGLVDCFRLFEQPEKSFSWWDYRMMAFRRNAGLRIDLI
                     LASAEMAKKCAESRIDKAPRKLERPSDHTPVLARFDI"
     gene            6099..7091
                     /locus_tag="PL152_02405"
     CDS             6099..7091
                     /locus_tag="PL152_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48157.1"
                     /translation="MRYRRSDYDVTNTVQVSSVASVKRAVEDLYSQTWPSGQVDRVSA
                     AFGDFERLFTGQFPGYFGCDTVYHDLQHSLDDTLAMARLLAGYDRSHTPEQRLGPERA
                     AMGLVTALFHDSGYIRQTDDTQHRNGAEFTLTHVGRSARFLARYLPTIGMAEWVPVAT
                     QIVHYTGYELPFDQIRLDDPRDRKVGHLLGTADMIAQMSDRCYLEKCRDRLYAEFVLG
                     GIAVSTSADGGLKVTYGSGLDVLRQTPEFVADTMRTRLDGEFGGAYRYAEPLFGGRNP
                     YMEAIDRTLSYLKQVLHSKRWSMLRREPPCFTWEKNPLQNTRTLVIDRLKAAVA"
     gene            complement(7148..7687)
                     /locus_tag="PL152_02410"
     CDS             complement(7148..7687)
                     /locus_tag="PL152_02410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HQR48158.1"
                     /translation="MTVAICVFCGSAQGNDPAFGAAARAVGRDIARRGWTLVYGGGRV
                     GSMGALADAALEAGGRVIGVIPRFLYEWEVGHDGLTEIEIVETLTERKVLMGDLGDAF
                     LTLPGGIGTMDEMFEALSWAQLGLEAKCNGLLNVNGYYDQLVAYLDHAVASGFLKPQH
                     RALLHVSGDPVALLDALGK"
     gene            complement(7684..8457)
                     /gene="trpA"
                     /locus_tag="PL152_02415"
     CDS             complement(7684..8457)
                     /gene="trpA"
                     /locus_tag="PL152_02415"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HQR48159.1"
                     /translation="MGKPRDTISAAIRARAATGRPALVGFMTAGFPDRGSFRRSLTAI
                     GDTADVVEIGVPFSDPMADGMTIQRASHVALQAGFTLPWLLDELGAMQRPKSPLLLMS
                     YLNPLLAYGLDRLPEAAVRSGVSGFIIPDLPYEESAETRAAFDREGLALVQMVTPVTP
                     DARLRMLCAASQGFVYAVTMTGTTGKSVAVPDEVLSYMDRVRAVSPVPVCAGFGIRGR
                     EQVSRMTGHVDGVVVGSALVEVLERGEDPAAFLRGLAGA"
     gene            complement(8457..9701)
                     /gene="trpB"
                     /locus_tag="PL152_02420"
     CDS             complement(8457..9701)
                     /gene="trpB"
                     /locus_tag="PL152_02420"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HQR48160.1"
                     /translation="MRAEALKVDRDTLLAQTLAGAFPDARGRFGPFGGRYVPETLVPA
                     LDRLQEGVRTILPDPAFRAEFESELRTWVGRPTPLTHARELSRRWGAEVWLKREDLAH
                     TGAHKINNAIGQALLAKRLGARRIVAETGAGQHGVASAAACARLGLPCVVYMGAVDME
                     RQAPNVGRMKLLGAEVVPVTGGDQTLRAAIDEALRDWVSDPDGTYYLLGSAVGPHPYP
                     YLVREFQSVIGVEARAQMLEQAGALPDVTVACVGGGSNAIGLFHPFVRDLGVKLLGFE
                     AGGTGTGLGQNAASLAYGRPGVLQGCYSLLLQDEHGQIQETHSVSAGLDYPGVGPEHA
                     LLATIGRVIYEAAEDDESLAALAECCQAEGILPAIESAHAFAGAKRYARANPGSRILI
                     GLSGRGDKDMPTLQRTLLRGKV"
     gene            complement(9698..10321)
                     /locus_tag="PL152_02425"
     CDS             complement(9698..10321)
                     /locus_tag="PL152_02425"
                     /inference="COORDINATES: protein motif:HMM:NF012900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HQR48161.1"
                     /translation="MDRLWIKVCGLRTRAAIEAAAGAGADAVGFVFHADSPRNLSVAE
                     ASDLQGAVPAGIERVAVFLRPTAAQVLEVIRVIRPDCIQADVEAMAGLGLPAGQRVLP
                     VLRNENVLPDPPPAQVLFEGARSGTGERADWSQATRVARRTQVVLAGGLDAGNVADAV
                     RMVRPFGVDVSSGVESIRGTKDPAMIREFVRAAREAGRALAAEEYRR"
     gene            complement(10327..11151)
                     /locus_tag="PL152_02430"
     CDS             complement(10327..11151)
                     /locus_tag="PL152_02430"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HQR48162.1"
                     /translation="MGLLSDMVRSSYERVAQARERAPDAALRAAALATPEPPRLKLDR
                     EGFDVIAELKLRSPSVGDLSARTIDPVRRLEAYAEGGATICSILTEPTRFDGDLEHLR
                     LAADVLQPYGVPAMRKDFIVDPYQVLEARAYGASGILLIVRMVPRERLTALMDAAADL
                     GLFVLLEAFSASDLKAAGEVASQREGRDEQVLMGLNCRDLETLEIDFGRFAELRDRMP
                     PDWPCVAESGVQTPEDAARVASLGYRFALVGTSLMRRADAKQAVREIVAAGRAGRA"
     gene            complement(11168..12205)
                     /gene="trpD"
                     /locus_tag="PL152_02435"
     CDS             complement(11168..12205)
                     /gene="trpD"
                     /locus_tag="PL152_02435"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HQR48163.1"
                     /translation="MADLRDMLERLLQRRDLAEPEAGELLLALTTGEVAPAMAGALLA
                     ALRSKGVTADEVRGFASAMRRLARRPNLPPGPPAIDIVGTGGDASGSFNISTGASLLV
                     AAMGLRVVKHGNRSISSRSGSADLLESLGLPLPLDERAAGDCLAATGFTFLFAPHYHP
                     ATKAVAPVRRDLGVRTVFNLLGPLTNPAEPPFSLIGAYSADAAKLMAETLAGMSVERA
                     FVIHGEPAWDEATPIGPFELYDVRPGRAVRELRDPRDLGVARCSVEDLAGGDPAHNAA
                     ALRAVFEGRDRGPHRDAIVLNTALALEVTGAASSAAEGMQAAAAAIDRGDGARFLERL
                     AAFGQSLAVTR"
     gene            complement(12216..12815)
                     /locus_tag="PL152_02440"
     CDS             complement(12216..12815)
                     /locus_tag="PL152_02440"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019661509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HQR48164.1"
                     /translation="MRAEPRLLLVDNYDSFTYNLVQAFLVLGADVTVRRNDTITVAEA
                     LALEPTHLCISPGPGTPRDAGVSIEMIRAMAGRIPVFGVCLGHQSIVDALGGETVRAG
                     RLMHGKVSMVEHDGRGIFTGVPQPFAAGRYHSLIARPDTLPAALEVSAKTAEGEIMAV
                     RHRELTVDGVQFHPESILTPDGPTLMRNFLATSGGHRAG"
     gene            complement(12812..14200)
                     /locus_tag="PL152_02445"
     CDS             complement(12812..14200)
                     /locus_tag="PL152_02445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HQR48165.1"
                     /translation="MTTAMFDIAADLDTPVSAYLKLKPFRPRFLLESVEGGERLARYS
                     FIGFGDCLEFRLDATGLRIGADRLERPANRDELLAALRRALAAAPHPDVASEGFPLLG
                     GLVGVASYDLVRYFERLPSIARAGDDCPDAHYVAPRSLLVFDHLTRRAALLHAGTEQE
                     RQALRREIIRALRGGLPGPAWAAKFGPAAGSLSEDEFLDRVARTKDYIAAGDVYQLVL
                     SVRFSGQCDLDPFEAYRALRLLNPSPYMYYCELGERVVVGSSPEALVKLNGRAAQMRP
                     IAGTRPRGADATEDTALEAELLADPKENAEHVMLVDLARNDLGRVATAGSVHVQPFRS
                     IERYSHVMHIVSGVRGELAPAKDAFDLFAAAFPAGTLVGAPKVRAMEIIDELEPVRRG
                     FYGGTVGYFGRGGDMDQAIAIRTMVFHGDEYSFQAGAGIVADSVPVTEYREVLAKSAV
                     LRRALELAREGL"
     gene            14496..>15391
                     /locus_tag="PL152_02450"
     CDS             14496..>15391
                     /locus_tag="PL152_02450"
                     /inference="COORDINATES: protein motif:HMM:NF005453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HQR48166.1"
                     /translation="MGGIPLDRIATVFEAERETYIERHRRSLQQTGHGIAGFYDGVPM
                     HWMRDWPMPFPFLVEEARGATLRDVDGNEYADFCLGDTGSMFGHSPPAVAEAIARQAT
                     RGLTYMLPAADSLAIGNLLAERFGLPNWQVATTATDANRFALRAARAATGRPKILVFN
                     GCYHGTVDETFVRLVNGRAVNRPGLLGQVMDLTQFARVVEFNDLPALEEALSHRDVAC
                     VITEPVLTNSCMVLPEPGFHAELRRLTRQAGTLLLIDETHTISTGPGGYTRAHGLEPD
                     LFVLGKPVAGGVPASVWGFTDEV"
     assembly_gap    15392..15491
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15492..15680
                     /locus_tag="PL152_02455"
     CDS             <15492..15680
                     /locus_tag="PL152_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48167.1"
                     /translation="GTEAEAAHAPALERAMHLSLLNRGCLIAPFHNMMLTSPVTTAAQ
                     VDALAAAFDESAARLAGL"
     gene            15697..17559
                     /locus_tag="PL152_02460"
     CDS             15697..17559
                     /locus_tag="PL152_02460"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR48168.1"
                     /translation="MRNDLLIRNALVFDGTGSPPRREDLAVRGGRIAARGAGLDPSGA
                     DRVVDASGLWLMPGLLDIHTHLDLEVEVAPGLPESVRHGTTTVVVGNCSLGTAFGAQR
                     RNGEDPILDCFARVENIPKSVLRKVVDRIDWSTTAGYLAHLNSLPLGPNIVPLIPHSM
                     LRIEAMGTAAAVSREPSDAELARMRELLEAAMNQGYAGFSTDAIPFHYLANAPHTDKR
                     IPTQYAGTGELRSLLDIVRSHDRVWQCTPDAARRFTTFLRFFFTSGRLFRRPLRTSAL
                     TAVDLTHERRTWRLFPWIARLLNSRLVDGRFHFQVLGTPFRMYAEGAICPIFEEFTST
                     RQLMSLEIEDAEGRRGLMATPDFGAVFVREWHDRGAVSTFNRDLDALHVERCPVPEWC
                     GETFAAVLRRLRDFQSGNAGAARSAAESEAFASFPPRIARDGEFLLHLLRRYDRDLRW
                     WFVVANDRPEILKRLLFDEHLLPGFNDSGAHLINLAFFDANLLTLQVAARESPELVAR
                     AVRRLTREPAEFFGVDAGRIDPGAQADLVLVDPQALLRYDTDANRRMVRRDIFEHDQL
                     VNRSDGVVTAVFIAGEQVWDGHDFTAAFGNRRLGRVLTAGGGVERCDHAARTAA"
     gene            complement(17726..19021)
                     /locus_tag="PL152_02465"
     CDS             complement(17726..19021)
                     /locus_tag="PL152_02465"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR48169.1"
                     /translation="MKLKGWAVVVVATSLAAPRAAGAETLAEAWALAEAQDNGLAAVR
                     SQAEAADLDAAAARAQRWPTLAVGGAYTRLDDSPAFDFGFTGLPIKPPELFGGDDFWM
                     GAATLTVPLFTSGRISSSIAAAEARGRGAGAQAAGAEEDMKLSVAEAYVGVLRSRKAL
                     EVADSNVRTLESIVGDVSAMFDRELVPKSDLLSVQVALADARQSRLKAANAAELALAS
                     YNRRLGEPLSRPVDLDESLPAPQGLPATPDELVRDAVERRTELKALDEQAVAYGQLAK
                     AERARVLPQVNVTGAYNYLENTFLDDQDFASVGIGLQWALFDGGQARKRAAALDRSKR
                     ATEQQRADTQSLIELQVRQAWLDVEETRERVHVTAGAVDQADENLRIARERYGAGLGT
                     QTQLLEAESLRVQALRNRDNAVLDAGLARLRLARAVGQL"
     gene            complement(19035..>19625)
                     /locus_tag="PL152_02470"
     CDS             complement(19035..>19625)
                     /locus_tag="PL152_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR48170.1"
                     /translation="GVEDGVMTVAEHAEDGSVKKEHKLPFKYSVVIPAFKGVDPVAAV
                     PELCNPRGFVLIDEHQRSKKYRNIFSAGVCVAIPPVEVTPVGTGAPKTGYMIESMVTA
                     ICENVAAELKGQPANARGTWNAICLADFGDTGAAFVALPQIPPRNVTWAKQGKWVHLA
                     KVAFEKYFLRKVKTGSVAPLYEKYVLSTIGIESLKQ"
BASE COUNT         2938 a         6966 c         6615 g         2906 t
ORIGIN      
        1 aactccgacg aggcgctgcg cgaggtcgcg ctcgacctcg aggaaggcgc cgacatggtg
       61 atgatcaagc ccggcctgcc ctacctcgac atcgtccggc gcgtgaagga ccggttcggc
      121 gtgcctacct tcgtgtacca ggtgagcggc gagtacgcga tgctcatggc cgccgcgggc
      181 aacggctggc tcgacgagcg cgcggtggtg ctcgaggcgc tcacctccat caagcgagcc
      241 ggcgcggacg ggatcctgac gtacttcgcg cgcagggcag cgcagtggct caaggaaatc
      301 aacccttgat ccgacgcgaa cctccacctg cgacccgctg atcgccagcc accactgcat
      361 cgccgcctgc catgtccgcc gcctacggcc tgttcggata cccggtccag cacagctggt
      421 cgccgttcat ccacggcatg ttcgcgaaac agaccggcca ggagatgacc tatcgcctgt
      481 tcgagtcgcc accggagcgc ttccgcagcg agatcctcgg gttcctcggc accggcggcg
      541 gcagcggcgc gaacgtgacc ctgccccaca agcaggcggc gacggaactc gtcaatgagc
      601 tgacggcgcg ggcgcagatg gccgacgcca tcaacaccat cgtgcgcacc gagtccgggc
      661 tgctcggcga caacaccgac ggtgccggcc tgctcacgga cctgacgcgc aatctcggcc
      721 tcggctggtc cgccccgcgc atcctggtgc tcggcgcggg cggcgcggcc cgtggcgcca
      781 tcggtccgtt gctctccctc aagccgcgca tgatcgtgat cgcgaatcgc accgccgcgc
      841 gggcggaaca gctcgcgtgg gaattccggg atttcggcgc ggtgagcggc tgcgagtact
      901 cgcgcctcga gaaccagcgc ttcgacctga tcgtgaacgc cacggccgcg agcctgcgcg
      961 gggaagtgcc gctgatcccg atcggcgtgg tcgactcgat gacgacctgc tatgacatgg
     1021 cttacggcgt cggcgacacc gcattcgtcg cgtgggcgaa gcgcatcggc gccggccgcg
     1081 ccgagcaggg ctggggcatg ctcgtcgagc aggctgcgga agcgttcgag ctctggcgcg
     1141 gcgtgcggcc cgacacggcg cccgtgctgc aggcgctgca caagcgcgcc gctacgggcc
     1201 ctcgtaatcc ttcttcaggt tgacgtacca cgcggccatg atggggatgc gcgccacctc
     1261 cgccggcgtg agttcccttg cgcgacgtgc cgggttcccg acgtagaggc cgcccgactc
     1321 gagccgcttg ccgggcgtga cgactgcgcc ggcgccgatc gtcacgtcgt cctcgacgac
     1381 gacgccgtcg aggacgatcg ccccgatccc caccagcacg cggttgccga tggtgcagcc
     1441 gtgcaccacg accttgtgcg cgatcaccac gtcctcgccg atcagcagcg gccagccggt
     1501 gtcatttccc ggatacggcc gggacacgtg cagcacgctg ccgtcctgca cgttgctgcg
     1561 ggcaccgatg cggatctggt tgacatcccc gcgcacgacc gccatcggcc agatggagac
     1621 gtccgcgccc gtctcgacgt cgccgatgac gagtgcggcc gggtcgacgt aggtgccggg
     1681 gccgagtttc ggccggaggt cgagataggg caggacgggc atgcgaagcc gcttgcggaa
     1741 ttgagacccc tagtctagac gctcggggcc gggaccctgc aatcttgccg ggcggcggcc
     1801 acaccggacc gccggggaga ccgatcgatg atcgtcgtgc tgctgctcct ggtcgcgctg
     1861 ctggtggtgg cggccgtgta cgtggtccgc atcttcaacg gcctggtggc gctgcgggag
     1921 aacgtccgca aggcctgggc caacatcgac gtgctgctca cccagcgcca cgacgagctg
     1981 ccgaagctgg tcgagacctg caagcgctac atgggctacg agcgcgagac gctcgagcgc
     2041 gtcatgcagg cgcgcgcggc cctgttccag gcccagggcc ggggtgacgt cgccgcggtc
     2101 ggcgcggccg aacaacagct gcgcgacggg atcggccggc tgttcgcggt ggccgagaac
     2161 taccccgccc tgaaggccga cgagggcttc cggcacctgc agggccgcat cacggagctc
     2221 gaggagggca tcgcggaccg tcgcgagctc tacaacgaat cggtcaacct gctgaacatc
     2281 cgcatccaga ccttcccgga cctcgtcgtc gcgcgcctct tcgacttcaa ggccgcctcg
     2341 ctgctcgagt tcgcggagga gcagaagcgg gacgtggacc tgggcaagct gtttggctga
     2401 agcgtcggat ttctggttct gggtcatcgc cctgtcgttc gccgtcgcgg gcggaggctg
     2461 gctgtcgctg cgatcgctca ggatcgcgcg cctgctcgag gacaccccga cctcgcgcat
     2521 ccgctccgcc gcgcagggct acgtcgagct gtcgggccgc tgccgcgcga tggacggaac
     2581 gcgcaacgac gcaccgctca cgcagcggcc gtgcgtgtgg tggcgttatc ggatccagca
     2641 gaaatcggag tcgggcgcac cgggaaagcg ccgggagtcg tggaccacga tcaactccgg
     2701 ccgctcggag ctgccgttcc tgctcgacga cggcacgggc gagtgcatcg tccagccttc
     2761 gggtgccgag gtcgtcaccg gcgaatccac gacgtggtac ggcagcacgc cgtggcccac
     2821 gcaggcggcc ggcgcgggcc tgctgcattc gcaggagcat cggtaccgct atttcgagga
     2881 acgggtctac gagcacgagc agctctgcct gttgggcaag ttccagtcgc tcacttccac
     2941 cacggcgagc gactcccagg ccgaggtcgc ggcgctcctc gcggactgga aggacgacca
     3001 gcctgcactc gtcgagcgct tcgaccgtga ccgcgatggc cgcgtcagcc tggcggagtg
     3061 ggaacaggcg cgcgaggccg cccggcgtac cgtcgccgaa cgcgcnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnctgca ggcatcccag ctgccgcgat tctcggcgtt
     3241 cgacccggac cgggtcgagc ccgcgatccg gcaacttctc gacgacaatc gccgcgagat
     3301 cgcggcgctg ctcgacgacg gcgcctcggg ctgggacggg ctcgtcgcgc cgctggagcg
     3361 catgcagcat gcgctcgcct gcgcgtggtc gccggtgggt cacctgaacg gcgtcatgaa
     3421 cggggaccgg atccgcgccg cctacaacgc ctgcctgccg ttgctcaccg cgtatcacac
     3481 cgagcttgcg cagaacgcac ggctgtgcgc cgcttaccag cgcgtgtacg acaccgaggg
     3541 cgcccggctc gacgaggcgc agcgcacgct gctcgagcac gcgctgcgcg acttccggct
     3601 gtccggcgtg tccctgcccg acgaccgcaa gcgacgcttc ggcgaagtga tggagcggct
     3661 ggcgaccgtg caggcgaagt tcgacgagaa cgtcctcgac gcgaccaacg catggtcgcg
     3721 gcacatcgac ctggagtcgc aactcgacgg cctgccgccg accacggtcg agcgcgcgag
     3781 acgcgccgcc gccgagtcgg gccgtccggg ctggctgctc gcgctggacg cgcccaacta
     3841 ccaggcggtc atgacccacg cctcggacgc ggagctgcgt cgcgagttct acgaagcgtg
     3901 gacgacgcgc gcgtccgacc agggcccgca cgcgggacgg tgggacaaca cgggcctcat
     3961 ggccgagatc ctcgggctgc gtcacgaggc ggccaacctg gtcgggttcg ccgactacgc
     4021 gcagtactcg ctcgcgacca agatggcacg cgagaccggc gaggtcctgt cgttcctgcg
     4081 cgagctggcc cggctgagcc ggcccgcggc cgagcgcgag ttcgcggacc tcgagcgcta
     4141 ctcgggacgc aagctcgagg cgtgggacgt ggcgtactgg tcggagcggc tgaagcacga
     4201 gcgcttcgag gtatccgccg agtccctgcg gccgtatttc ccgctgccgc gcgtgctagc
     4261 gggactcttc accgtcgtct ccaggctgta ccgcatccgc atcgtggcgc gcgcgggcgc
     4321 cgacgtgtat cacccggacg tgcgctactt cgacgtgctc gatcgcgatg gcagcccgcg
     4381 cggcggcttc ttcgtcgatc tctacgcgcg cccgaagaag cgcggcggcg cgtggatgga
     4441 cgactgcgtc ggccgcatgc gcatcgggcc gtcgagcgcg ctgccggtcg cctacctggt
     4501 gtgcaatttc acgccacccg tgggggaccg cccgtcgctg ctcacgcacg acgaggtgct
     4561 cacgctgttc catgagttcg ggcacgggct gcaccacatg ctcacgcggg tcgactaccc
     4621 gagcgtcgcc ggcatcaacg gagtgccctg ggacgcggtc gagctgccga gccagttcat
     4681 ggagaacttc gcctggcgac acgaggtgct gccgctcatc tcggcgcacg tcgagaccgg
     4741 cgagccgctg ccgtcggcga ccctcgcgaa gctgcaggcg acgcggacct tccaggcggg
     4801 catgcagacg gtgcggcagc tcgaatttgc gctgttcgat ttccggctcc acgcggagca
     4861 cgtgcccggc gcgccgccgc ggatcgcgga gacgctcgag gaggtccgtc gcgaggtcgc
     4921 ggtggtgcgg ccacccgcgt tcaaccgctt cccgcacagc ttccagcaca ttttctccgg
     4981 cgggtacgcc gccggctatt acagctacaa gtgggccgag gtcctgtccg ccgacgcgtt
     5041 cggcgcgttc gaggagcacg gcgtctttga ccgggcgacg gcggcgagtt tcctgcgctc
     5101 gatcctcgag cgcggcggca gtcgcgacgc gatggaggca ttcgtcgagt tccgcggccg
     5161 caagccccgg ctcgagccgc tcctcaggca gatgggactc gcggcgtagc aatgctgctc
     5221 gccacctgga acgtcaactc gctgcgcgtg cgcttgccgc acctgcggga ctggcttgcg
     5281 gccaatccgc tcgacgtcct ggcattgcag gagaccaaga ttccggacgc ggactttcca
     5341 cgcggggaaa tcgagtcgct cggcatgcgc gccgtgttca gcggccagcg cacctacaac
     5401 ggggtcgcga tcctgtcgaa ggaggaaccg cgcgacgtcg tcgccggtat cccggcgttc
     5461 gacgacgagc agcggcgcgt gatcgcggcg acggtcggtg gcgtacgcgt gatcgacgtg
     5521 tatgtcccga acgggcagga agtcggttcg gagaagttcg agtacaagct gcgctggctc
     5581 gaggcgctgc gcggctacgt ggtcgcggaa ctggcgcggc atccgcggct cgtggtgctc
     5641 ggcgactaca acatcgcgcc ggaggaccgc gacgtgcacg acccgaaggc ctgggagggc
     5701 tcggtccacg tgagcgagcc ggagcgggcg gcgctgcgcg cactcgtcgc cgcaggtctc
     5761 gtcgactgct tccggctgtt cgagcagccg gagaagtcgt tttcgtggtg ggactaccgg
     5821 atgatggcat tccggcgcaa cgcaggtctc agaatcgacc tgatcctcgc gagcgcggaa
     5881 atggccaaaa aatgcgcgga aagtcgcatc gacaaggcgc cgcgcaagct cgaacggccg
     5941 tccgatcaca ccccggtgct cgccaggttt gacatatagg cgccgggcgc gcggtgtcgg
     6001 ttttctttac tctggcgttc cccatccgaa ggactacact cggtcgcatc caatgacgcc
     6061 cgcgacctcg gcccgccgct gacaacaaca atccggccat gcgatacagg cgcagcgatt
     6121 acgacgtgac gaacaccgtt caggtgagtt ccgtcgcgtc ggtgaagcgg gcagtcgagg
     6181 atctctattc gcagacctgg cccagcgggc aggtcgaccg tgtctcggcg gcgttcggcg
     6241 acttcgagcg gctgttcacc gggcagttcc ccggctactt cggctgcgac accgtatacc
     6301 acgacctgca gcattccctc gacgacacgc tcgcgatggc gcgcctgctc gccgggtacg
     6361 accgctcgca cacgcccgag cagcgcctcg gcccggagcg cgcggcgatg ggactcgtca
     6421 cggcgctgtt ccacgactcg ggctacatcc ggcagacgga cgacacgcag caccgcaatg
     6481 gcgccgagtt cacgctcacg cacgtcggtc gcagcgcgcg gttcctcgcc cgctatctcc
     6541 cgaccatcgg catggccgag tgggtgcccg tcgccacgca gatcgtgcac tacacgggct
     6601 acgagctgcc cttcgaccag atccggctcg acgacccgcg cgaccgcaag gtcggccacc
     6661 tgctcggcac cgcggacatg atcgcgcaga tgtcggaccg ctgttacctc gagaagtgcc
     6721 gcgaccggct ctacgccgag ttcgtactcg gtggcatcgc ggtctcgacg agtgcggacg
     6781 gcggactcaa ggtcacctac ggttccgggc tcgacgtgct gcgccagacg ccggagttcg
     6841 tggcggacac catgcgcacg cggctcgacg gcgagttcgg cggcgcgtac cgctacgccg
     6901 agccgctgtt cggcggccgc aacccgtaca tggaagcgat cgaccgcacc ctttcgtacc
     6961 tgaagcaggt cctgcactcg aagcgctggt cgatgctgcg gcgcgagccg ccctgcttca
     7021 cctgggaaaa gaatccgctg cagaacacgc ggacgctggt catcgaccgg ctcaaggccg
     7081 ccgtcgcctg aaattagaaa taggggacat tctcctaatt cccgagcggg aaataggaga
     7141 atgtccccta ttttcccagg gcgtcgagca gcgccacggg gtcgccggac acgtgcagca
     7201 gcgcgcggtg ctgcggcttg agaaatcccg acgcgacggc gtggtcgagg tacgcgacca
     7261 gctggtcgta atagccgttc acgttgagca ggccgttgca cttggcctcg agtccgagct
     7321 gggcccagct cagtgcctcg aacatttcgt ccatcgtccc gatgccgccc ggcagcgtga
     7381 gaaacgcgtc gccaaggtcg cccatgagga ccttcctctc ggtcagcgtc tcgacgatct
     7441 cgatttccgt gagcccgtcg tggccgactt cccactcgta gaggaaccgc gggatcacgc
     7501 cgatcacgcg accgcccgcc tcgagcgcgg cgtcggcaag cgcgcccatc gaccccaccc
     7561 ggcctcctcc gtagaccagc gtccagccgc gccgggcgat gtcccggccg acggcacggg
     7621 ccgccgcgcc gaacgccgga tcgttgccct gcgccgaacc gcagaagacg cagatcgcga
     7681 cggtcacgcg cccgccagtc cccggaggaa cgcggccggg tcctcgccgc gctcgagcac
     7741 ctcgaccagc gccgatccca ccacgacgcc gtccacgtgc ccggtcatgc gggacacctg
     7801 ttcgcgcccg cggatgccga agccggcaca cacgggaacc ggcgacaccg cgcgcacgcg
     7861 atccatgtac gacagcactt cgtccggcac ggcgacgctc ttgccggtcg tgccggtcat
     7921 ggtcaccgcg tagacgaagc cctggctcgc ggcgcacagc atccgcaatc gcgcgtcggg
     7981 cgtgaccggc gtgaccatct gcaccagcgc cagtccctcg cggtcgaacg ccgcgcgggt
     8041 ctcggcgctt tcctcgtacg gcaggtccgg gatgatgaat ccggagacac ccgatcgcac
     8101 ggccgcctct ggcaggcggt cgaggccata ggcgagcaac ggattgaggt agctcatgag
     8161 cagcagggga gacttcggcc gttgcatcgc gccgagctca tccagcagcc acggcagcgt
     8221 gaatcccgcc tgcagcgcga cgtggctcgc gcgctggatg gtcatgccat cggccatggg
     8281 atcggagaac ggcacgccga tttccaccac atccgcggtg tcgccgatcg ccgtgagact
     8341 gcgccggaaa ctcccgcggt ccggaaagcc tgccgtcatg aagcccacga gggccggtcg
     8401 cccggtcgcg gcccgtgcgc ggatcgcggc gctgatcgtg tcgcgcggct tgcccatcag
     8461 accttccccc tgagcagcgt gcgctgcagc gtcggcatgt ccttgtcgcc gcggccggac
     8521 aacccgatca ggatgcgact gccgggattc gcacgcgcgt agcgcttcgc ccccgcgaac
     8581 gcgtgcgcgc tctcgatcgc cggcaggatg ccctcggcct ggcagcactc ggcgagcgcc
     8641 gcgagcgact cgtcgtcctc ggccgcctcg taaatcacgc gaccgatcgt ggcgagcagg
     8701 gcgtgctcgg gccctacacc ggggtagtcg aggcctgccg acaccgagtg ggtctcctgg
     8761 atctgaccgt gctcgtcctg cagcaacagc gagtagcagc cctgcagcac gccgggccta
     8821 ccgtacgcaa gcgatgcggc gttctgcccg aggcccgtgc ccgtgccgcc ggcctcgaag
     8881 ccgagcagct tcacgccgag gtcgcgcacg aacggatgaa acagcccgat ggcgttggag
     8941 ccgcccccga cgcatgcgac cgtgacgtcc ggcagcgcgc cggcctgctc cagcatctgc
     9001 gcacgggcct cgactccgat caccgactgg aattcgcgca cgaggtacgg atacgggtgc
     9061 ggtccgaccg ccgacccgag caggtagtac gtgccgtccg ggtccgacac ccagtcgcgc
     9121 agcgcctcgt cgatcgcggc gcgcagcgtc tggtcgccgc cggtcaccgg cacgacctcg
     9181 gcgccgagca gcttcatgcg cccgacgttc ggcgcctggc gctccatgtc caccgcgccc
     9241 atgtacacga cgcagggcag gccgaggcga gcgcaggcgg cggccgacgc gacgccgtgc
     9301 tgtccggcgc cggtctcggc gacgatgcgc cgggcgccga gccgcttcgc gagcagcgcc
     9361 tgcccgatcg cgttgttgat cttgtgcgcg cccgtgtgcg cgaggtcctc gcgcttgagc
     9421 cagacctcgg cgccccagcg acgcgacagc tcgcgcgcgt gcgtgagcgg cgtcgggcga
     9481 cccacccacg tgcgcagttc cgattcgaac tccgcgcgga atgccggatc gggcaggatg
     9541 gtgcgcacgc cttcctgcag ccggtcgagc gccgggacca gcgtctccgg cacgtaccgg
     9601 ccgccgaacg gcccgaatcg cccgcgcgca tccgggaatg cgcctgccag cgtctgcgcg
     9661 agcagcgtgt cccgatcgac tttcaatgcc tccgctctca tcgacgatac tcctcggctg
     9721 cgagggcccg tcccgcctca cgggcggccc tcacgaattc acgaatcatg gctggatcct
     9781 tggttccgcg aatcgattcg acgccggaac tcacgtccac gccgaacggg cggaccatgc
     9841 gcacggcgtc ggcgacgtta cccgcatcga gcccgcctgc gagcacgacc tgagtccgtc
     9901 gtgcgacgcg cgtcgcctgt gaccagtccg ccctctcgcc cgtcccgctg cgcgcgccct
     9961 cgaagagcac ttgcgcgggc ggcgggtccg gcaacacgtt ctcgttccgc aatacaggca
    10021 acaccctctg gccggccggc agcccgagcc cagccatggc ttcgacgtcg gcctggatgc
    10081 agtcgggccg aatcacacgt atgacctcga gcacctgcgc cgccgttggc cgcaggaaca
    10141 ccgccacccg ctcgatgccg gccggtaccg cgccctgcag gtcgctcgcc tcggcgacgg
    10201 aaagattgcg cggggagtcc gcgtgaaaca cgaatcccac ggcatcggcg cccgcacccg
    10261 ctgcggcctc gatcgctgca cgcgtcctca acccgcacac cttgatccag agcctgtcca
    10321 cgcgcttcaa gcccgcccgg cacggccggc cgcaacgatc tcgcggacgg cctgcttcgc
    10381 atcggcacgg cgcatcaggc tcgtgccgac cagcgcgaac cggtagccca gcgacgcgac
    10441 ccgcgcggcg tcctcgggcg tctgcacccc gctctccgcg acgcagggcc agtcgggcgg
    10501 catgcggtca cggagttctg cgaagcgtcc gaagtcgatc tcgagggtct cgaggtcgcg
    10561 gcaattgagt cccatcagca cctgctcgtc gcggccttcc cgctgcgacg cgacttctcc
    10621 cgcggccttg aggtcgctcg cgctgaacgc ctcgagcagc acgaacaggc cgaggtcagc
    10681 ggcggcgtcc atcaaagcgg tcaatcgctc ccgcgggacc atgcgcacga tgagcaggat
    10741 gccggacgcg ccgtacgcgc gcgcctcgag cacctggtac gggtccacga tgaagtcctt
    10801 gcgcatcgcc ggcacgccgt acggctgcag cacgtcggcg gcgagccgca ggtgctcgag
    10861 atcgccgtcg aagcgcgtcg gttccgtgag gatcgagcaa atcgtggcac cgccctcggc
    10921 ataggcctcc aggcgtcgca ccgggtcgat cgtccgcgcc gacaggtcgc cgacggatgg
    10981 cgagcgcagc ttcagctccg cgatcacgtc gaagccctcg cgatcgagct tgagccgggg
    11041 cggctctggc gtcgcaagcg ccgccgccct cagcgccgca tccggcgccc gctcgcgtgc
    11101 ctgcgcgacg cgctcgtagc tcgaacggac catgtcggac agcaggccca aggagtgtgt
    11161 cccgtgatca gcgagtgacg gccagtgact gaccgaacgc ggcgaggcgc tcgaggaatc
    11221 gcgcgccgtc cccgcgatcg atggctgcag cggccgcctg cattccctca gcggcggacg
    11281 aagccgctcc cgtgacctcc agcgcaaggg cggtgttgag cacgatggca tcgcggtgcg
    11341 gcccgcgatc gcggccctcg aacaccgcgc gcaatgcggc tgcgttgtgc gccgggtcgc
    11401 cgccggccag gtcttcgacc gagcagcgcg ccacgccgag gtcgcgcggg tcccgcagct
    11461 cgcgcaccgc gcgtccgggc cgcacgtcgt acagctcgaa gggaccgatc ggcgtcgctt
    11521 cgtcccacgc cggctcgccg tggatgacga acgcccgctc gaccgacatg ccggccaggg
    11581 tctcggccat gagcttcgcg gcatccgcac tgtaggcgcc gatcaggctg aacggcggct
    11641 cggccgggtt cgtcagcggg ccgagcaggt tgaacaccgt gcgcacgccg aggtcgcggc
    11701 gcaccggcgc cactgccttc gtcgcagggt ggtagtgcgg cgcgaacagg aaggtgaacc
    11761 cggtcgccgc gagacagtcg ccggccgcgc gttcgtcgag cggcagcggc agcccgagcg
    11821 attcgagcag gtcggcgctg cccgagcggc tcgaaatcga gcgattgccg tgcttcacga
    11881 cgcgcaggcc catggccgct acgagcagcg aggcgccggt ggagatgttg aagctgccgg
    11941 acgcatcgcc gccggtgcca acgatgtcga tcgcaggcgg tcccggcggc aggttcggcc
    12001 ggcgtgcaag ccggcgcatc gcactcgcga agccgcgcac ctcgtcggca gtcacgccct
    12061 tcgatcgcag cgcggcgagc agcgcgccgg ccatcgccgg ggccacttcc cccgtcgtga
    12121 gcgccagcag cagctcgcca gcctcgggct cggcgaggtc gcgccgctgc aggagtctct
    12181 cgagcatgtc gcgaaggtcg gccatcgtgg ttcgatcagc cagcacggtg cccgccgctg
    12241 gtcgcgagga aattccgcat cagcgtcggc ccgtcgggtg tcaggatgct ctccgggtgg
    12301 aactgcacgc cgtcgaccgt gagttcgcgg tggcggacgg ccatgatctc gccctccgcg
    12361 gtcttcgccg agacctcgag cgcagcaggc agtgtgtcag gcctggcaat caacgagtga
    12421 tagcggccag ccgcgaacgg ttgcggcaca cccgtgaaga tcccgcgacc gtcgtgctcc
    12481 accatcgaca ccttgccgtg catcaggcgc ccggcgcgga cggtctctcc gcccagcgcg
    12541 tcgacgatgg actggtgacc gaggcagacg ccgaacacgg gaatgcgacc cgccatggcc
    12601 cggatcatct cgatcgacac cccggcgtcg cgcggcgtgc ccggtcccgg ggagatgcac
    12661 aggtgcgtgg gctcgagcgc gagcgcttcg gcgacggtga tcgtgtcgtt gcggcggacg
    12721 gtcacgtccg cgccgagcac gaggaacgcc tgcacgaggt tgtaggtgaa gctgtcgtag
    12781 ttgtccacga gcagcaggcg cggctcagct ctcacagtcc ctcccgcgcg agttccaggg
    12841 cgcgcctcag cacggcgctc ttggcgagca cctctcggta ctcggtgacc ggcacgctgt
    12901 ccgccacgat gcccgccccg gcctggaagc tgtactcgtc gccgtggaac accatggtgc
    12961 ggatggcgat cgcctggtcc atgtcgccgc cgcgcccgaa atacccgacc gtgccaccgt
    13021 agaaaccgcg gcgcacgggt tccagctcgt cgatgatctc catcgcgcgc accttgggcg
    13081 cgccgaccag cgttccggcc gggaacgctg cggcgaacag gtcgaacgcg tccttggccg
    13141 gcgcgagctc gccgcgcacg ccgctcacga tgtgcatcac gtggctgtag cgctcgatcg
    13201 agcggaacgg ctgcacgtgc acggagccgg cggtcgccac gcggccgagg tcgttgcgcg
    13261 cgaggtccac cagcatcacg tgctcggcgt tctccttggg gtccgcgagc aactcggcct
    13321 cgagcgccgt gtcctcggtt gcgtcggccc cgcgcgggcg cgtgccggcg atcggtcgca
    13381 tctgtgccgc ccgcccgttc agcttcacga gcgcctcggg cgacgagccc acgacgacgc
    13441 gttcgccgag ttcgcagtag tacatgtagg gggaaggatt cagcaggcgc agcgcgcggt
    13501 acgcctcgaa cgggtcgagg tcgcactgtc ccgagaatcg caccgacagc acgagctggt
    13561 agacgtcgcc ggccgcgatg tagtccttgg tgcgggccac gcggtcgagg aattcgtcct
    13621 cggagagact ccccgccgct gggccgaact tggccgccca ggccggcccc ggcagcccgc
    13681 cgcgcagcgc acggatgatt tcgcggcgca gcgcctggcg ctcctgctcc gtgcccgcgt
    13741 gcaggagcgc ggcacgccgc gtcaggtggt cgaacaccag cagcgatcgc ggcgcgacgt
    13801 agtgggcgtc ggggcagtcg tcgccggcac gcgcgatcga cggcaggcgc tcgaaatagc
    13861 gcacgaggtc gtagctcgcc acgccgacca ggccgccgag cagcgggaag ccctcgctgg
    13921 cgacgtcggg atggggtgca gcggccagtg cgcgccgcaa ggccgcgagg agttcgtccc
    13981 ggttggccgg gcgttccagc cggtcggcgc cgatgcgcag ccccgttgcg tcgaggcgga
    14041 actccaggca gtcaccgaac ccgatgaacg aatagcgcgc gaggcgctcg ccgccctcca
    14101 cgctctccag caggaagcgc ggccggaacg gcttcagctt gaggtaggcc gacaccgggg
    14161 tgtcgaggtc ggcggcgatg tcgaacattg cggtcgtcac tgacggttcc tctgtcgcga
    14221 ggacccgggg acgcacaaaa aaacccatcc ccggttggct cgcggagacg ggtcctggat
    14281 tcctgcgaac gagacgtgcg cgtccggccc cactcctagg agtggcgcca ccaccagcgg
    14341 tgattggccg tgtttccggg tcgcgcgttc atgttgcagg gcagtatcca cgggccggcg
    14401 ggacgggtca aggcaggccg tgatcgcgga cgggcggcca gcctacaatc gagcacccgg
    14461 gcatacacag gacaggcacg gtgtcggaaa ggaccttggg cggcattccg ctcgaccgca
    14521 ttgccacggt gttcgaggcc gagcgggaaa cttacataga gcggcaccgg cgctcgctgc
    14581 agcagaccgg ccacggcatc gcgggcttct acgacggcgt gccgatgcac tggatgcgcg
    14641 actggcccat gccgttcccg ttcctcgtcg aggaggcgcg cggcgccacc ctgcgcgacg
    14701 tcgacggcaa cgaatacgcg gacttctgcc tgggcgacac gggctcgatg ttcgggcact
    14761 ccccgcccgc cgtcgcggag gcgatcgcgc gccaggcgac ccggggcctc acgtacatgc
    14821 tgccagcggc ggattcactg gcgataggca atctgctggc cgagcgcttc gggctgccga
    14881 actggcaggt cgccaccacc gcgaccgacg cgaatcgctt cgcactgcgc gcagcgcggg
    14941 cggcgaccgg ccggccgaag atcctcgtat tcaacggctg ttaccacggc accgtggacg
    15001 agacgttcgt gcgactcgtg aacgggcgtg ccgtgaaccg cccgggcctg ctcggccagg
    15061 tgatggacct gacgcagttc gcccgcgtcg tcgagttcaa cgacctgccg gcgctcgaag
    15121 aggctttatc gcatcgggac gtcgcctgcg tcatcaccga gcccgtgctc acgaattcgt
    15181 gcatggtgct gcccgagccc ggcttccacg cggaactgcg tcgcctgacg cggcaggccg
    15241 ggacgctgct gctgatcgac gagacgcaca cgatttcgac gggtcccggc gggtacacgc
    15301 gagcgcacgg cctcgagccc gacctgttcg tgctcggcaa gcccgtcgcg ggcggcgtgc
    15361 ccgcaagcgt ctggggattc accgacgagg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15421 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15481 nnnnnnnnnn nggcacggag gcggaagccg cccacgcccc tgcgctcgaa cgggcgatgc
    15541 atctgtcgct gctgaaccgc ggctgcctga tcgcgccctt ccacaacatg atgctcacga
    15601 gcccggtgac cacggccgcg caggtcgacg cgctcgccgc cgcgttcgac gagtcggccg
    15661 cccgtctcgc cggtctctga gtccgcctgt cacaccatgc gcaacgacct gctgatccgc
    15721 aatgcgctcg tcttcgacgg gacgggcagc ccgccccgcc gtgaggacct ggccgttcgc
    15781 ggcggccgca tcgccgcgcg tggcgccggt ctcgacccgt ccggcgccga ccgcgtcgtc
    15841 gacgcatccg gtctctggct catgccggga ctgctcgaca tccacacgca cctcgacctc
    15901 gaagtggagg tcgcgccggg gctgcccgaa tcggtgcgtc acggaaccac gaccgtcgtg
    15961 gtcggcaact gcagtctcgg caccgcattc ggggcccagc gcaggaacgg cgaagacccg
    16021 atcctcgatt gcttcgcacg cgtcgaaaac atcccgaagt ccgtgttgcg caaggtggtc
    16081 gaccgcatcg actggtccac gacggccggt tacctcgcgc acctgaattc gctgccgctc
    16141 ggaccgaaca tcgtgccgct catcccgcac tccatgctgc gcatcgaagc catgggcacc
    16201 gcggccgccg tgagccgcga accaagcgac gcggagctcg cgcggatgcg ggagctgctc
    16261 gaggccgcga tgaaccaggg atacgcgggc ttctccacgg acgcgatccc gttccactac
    16321 ctcgccaacg ccccgcacac cgacaagcgg atcccgacgc agtacgcggg cacgggggag
    16381 ctgcggtcgc tgctcgacat cgtgcgcagc cacgaccgcg tctggcagtg cacacccgac
    16441 gcggcccggc gcttcaccac gttcctgagg ttcttcttca cgagcggacg gctcttccgc
    16501 cgtccgctgc ggacctccgc gctgacggcc gtggacctga cgcacgagcg ccgcacgtgg
    16561 cggctgttcc cgtggatcgc gcggctcctc aactcccgac tggtcgacgg ccgcttccac
    16621 ttccaggtgc tcggcacgcc attccgcatg tacgcggaag gcgccatctg ccccatcttc
    16681 gaggagttca cctccacgcg gcagctcatg tcgctcgaga tcgaggatgc cgagggccgt
    16741 cgcggtctca tggcgacgcc ggacttcggt gcagtcttcg ttcgcgagtg gcacgaccgc
    16801 ggcgcagtct ccacattcaa tcgcgacctc gacgcgctgc acgtcgaacg ctgcccggtg
    16861 ccggagtggt gcggcgagac ctttgccgcg gtcctgcgac ggctgcgcga tttccagtcc
    16921 gggaacgccg gtgccgcccg cagcgcggcc gagtccgagg cattcgcgtc gttcccaccg
    16981 cgcatcgcgc gcgacggcga gttcctgctg cacctcctgc ggcgctacga ccgcgacctg
    17041 cgctggtggt tcgtcgtcgc gaacgaccgc ccggagatcc tgaagcgcct gctgttcgac
    17101 gagcacctgc tgcccgggtt caacgacagc ggcgcgcacc tgatcaacct cgcgttcttc
    17161 gacgccaacc tgctcacgct gcaggtcgcc gccagggagt cgccggaact cgtcgcgcgc
    17221 gccgtgcggc gcctgacccg ggagcctgcg gagttctttg gcgtggacgc cggccgcatc
    17281 gatccgggcg cgcaggccga cctcgtgctc gtggacccgc aggcgctgct ccgctatgac
    17341 accgacgcga accgccgcat ggtccggcgc gacatcttcg agcacgacca gctggtgaac
    17401 cgctccgacg gggtggtgac agcggtcttc atcgcgggcg aacaggtctg ggacgggcac
    17461 gacttcaccg ccgcgttcgg caatcgcagg ctcgggcggg tactcaccgc gggcggtggc
    17521 gtggaacgat gcgaccacgc agcccggaca gcggcttgac ggcaatcgaa acgcacaccg
    17581 cggcgagcag ggttcgaggg agcctatggt ggcggggcgt cacgcggcat ggacgccgcg
    17641 tgcgagccta cagggatgta tttgcggcgt ccccgccacc ataggctccc tcgaaccctg
    17701 gcgacgccct gctcatgcgc tcgacctaga gctggccgac ggcgcgggcg agccgcagcc
    17761 gcgcgagccc cgcgtcgagc accgcattgt cccgattacg cagtgcctgg acgcgcagcg
    17821 actccgcctc gagcagctgc gtctgcgtgc cgagtcccgc tccgtagcgc tccctcgcga
    17881 tgcgcaggtt ctcgtcggcc tggtcgactg cgcccgcggt gacgtgcacg cgctcgcggg
    17941 tctcctcgac gtcgagccac gcctggcgca cctgcagttc gatcagcgac tgcgtgtctg
    18001 cccgctgctg ttccgtcgct cgcttcgagc gatcgagcgc ggcggcgcgc ttgcgcgcct
    18061 gccctccatc gaacagcgcc cactgcagtc cgatcccgac ggatgcgaag tcctggtcgt
    18121 cgaggaacgt gttctccagg tagttgtagg caccggtgac gttcacttgc ggcagcacgc
    18181 gcgcgcgctc ggctttcgcc agctggccgt aggcgaccgc ctgctcgtcg agtgccttga
    18241 gttccgtgcg ccgctcgacg gcgtcccgca cgagttcgtc gggtgtcgcc ggcaggccct
    18301 gcggcgcggg cagggactcg tcgaggtcga ccggccgcga cagcggctcg cccaggcgac
    18361 ggttgtagga cgcgagcgcc agctcggccg cattcgccgc cttgagtcgc gactggcgcg
    18421 cgtcggccag tgcgacctgc accgagagca ggtcgctctt cggcacgagc tcgcggtcga
    18481 acatcgcgct cacgtcgccc acgatcgact cgagcgtgcg cacgttgctg tcggccacct
    18541 cgagcgcctt gcgcgagcgc agcacgccca cgtaggcctc ggcgaccgac agcttcatgt
    18601 cctcctcggc gcccgccgcc tgcgcaccgg ccccgcgccc gcgtgcctcc gcggcggcga
    18661 tgctcgacga gatgcggcca ctggtgaaca gcggcacggt cagggtcgcc gcccccatcc
    18721 agaagtcgtc ccccccgaag agctcgggag gcttgatggg caggccggtg aacccgaagt
    18781 cgaacgcggg cgagtcgtcg agccgagtgt aggcgccgcc gacggcgagg gtcggccagc
    18841 gctgcgcccg cgcggcggcg gcgtccagat cggccgcctc cgcctggctg cggacggcgg
    18901 cgagcccgtt gtcctgcgcc tcggcgagcg cccacgcctc ggccagcgtt tccgcgcccg
    18961 cggcgcgggg cgccgcgagc gacgtcgcaa cgacgacgac cgcccagccc tttagcttca
    19021 tccgtgcatt ctcgtcattg cttgagcgac tcgatgccga tggtgctcag gacgtatttc
    19081 tcgtagagcg gcgccaccga cccggtcttc accttgcgca ggaagtactt ctcgaacgcg
    19141 accttggcga gatggaccca cttgccctgc ttcgcccagg tcacgttgcg cggcgggatc
    19201 tgcggcagcg cgacgaacgc cgcgccggtg tcgccgaagt cggcgaggca gatcgcgttc
    19261 caggtgccgc gcgcattggc gggctggccc ttcagctcgg ccgccacgtt ctcgcagatc
    19321 gcggtgacca tcgactcgat catgtagccg gtcttcggcg ccccggtccc gacgggcgtc
    19381 acctcgacgg gcgggatcgc cacgcacacg ccggccgaga agatgttccg gtacttcttc
    19441 gaccgctggt gctcgtcgat cagaacgaag ccgcgcggat tgcacaactc gggcacggcc
    19501 gcgaccggat ccacgccctt gaaggccggg atgaccaccg agtacttgaa cgggagcttg
    19561 tgctccttct tcacgctgcc gtcctcggca tgttccgcca cggtcatcac gccgtcctcg
    19621 acgcc
//
LOCUS       DAOMFD010000021        19475 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_526_length_19475_cov_32.260196, whole genome shotgun sequence.
ACCESSION   DAOMFD010000021 DAOMFD010000000
VERSION     DAOMFD010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 19475)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19475)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19475
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_526_length_19475_cov_32.260196"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1345)
                     /gene="fabF"
                     /locus_tag="PL152_02475"
     CDS             complement(101..1345)
                     /gene="fabF"
                     /locus_tag="PL152_02475"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HQR48171.1"
                     /translation="MSKRRVVVTGLGIVSPLGSSVAKAWDAVCNGRSGIVALTRLDTT
                     AFPVRIGGEVRDFNAEDYMPAKDVRKFDPFVPFGFAAAVQAVKDSGIVVTEANAPRLG
                     VAIGAGIGGLSTIEENTAKWLEAKTPRKISPFFIPGSIINAAAGQVSIHYGMKGPNLA
                     MVTACTTSTHCIGIAARTIQYGDADVMVAGGAEAAFTPLGLGSFCQARALSTRNDDPA
                     AASRPWDRDRDGFVMAEGAGALVLEEYEHARARGATIYAEFAGFGMSGDAYHITAPPE
                     NGEGAYLAMANAVRDAGVTPADVQHINAHATSTELGDRAETVAIKRAFGDHAGRIAIS
                     STKSMTGHLLGAAGAVEAIFTILAIRDGIVPPTINLENPDPACDLDYVPGSARRMPLK
                     TALSNSFGFGGTNGSLVFRAPQ"
     gene            complement(1414..1656)
                     /gene="acpP"
                     /locus_tag="PL152_02480"
     CDS             complement(1414..1656)
                     /gene="acpP"
                     /locus_tag="PL152_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQR48172.1"
                     /translation="MSTIEQRVKAIVAEQLGVKEEEVNNDSSFVDDLGADSLDTVELV
                     MALEEEFETEIPDEDAEKITTVQQAIDYINERRSQA"
     gene            complement(1774..2514)
                     /gene="fabG"
                     /locus_tag="PL152_02485"
     CDS             complement(1774..2514)
                     /gene="fabG"
                     /locus_tag="PL152_02485"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HQR48173.1"
                     /translation="MLENELALVTGASRGIGRAIALELGRRGARVVGTATTGQGAAAI
                     SDALAAAGLKGRGVVLDVSSAASVESCFKEVEAAEGAPTILVNNAGITKDGLLMRMSA
                     EDWQAVLDTDLTAVYRTCKAVMRPMMKARRGRIVNIASVIAVMGNAGQCNYAAAKAGM
                     IGFSKSLAREVGSRGITVNVVAPGFIVTDMTDALNDEARKALLAQVPLGRLGSPDDVA
                     HAVAFLASPQAGYVTGETLHVNGGMYMI"
     gene            complement(2518..3459)
                     /gene="fabD"
                     /locus_tag="PL152_02490"
     CDS             complement(2518..3459)
                     /gene="fabD"
                     /locus_tag="PL152_02490"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HQR48174.1"
                     /translation="MSLAFVFPGQGSQSVGMLASLAAAEPVVRQTFDEASGVLGYDLW
                     ALCQDGPEADLAVTERTQPAMLAAGVATWRAWRGRGGPMPVAMAGHSLGEYTALVCSD
                     SLDFRTAVDLVRFRGQVMQRAVPLGTGAMAAILGLDDADVAAACAEAAGSEVVEPVNF
                     NAPGQVVVAGHATAVARAIEAAKARGAKRAVALPVSVPSHSSLMIGAADELAGRLATV
                     EVRMPSVPDVYTVDVQRHTSADGIRAALRQQLFKPVRWADTVRAMLASGVTTLVECGP
                     GKVLTTLNKRVERRADLKVLSIDDPDSLAAGLAAVQG"
     gene            complement(3504..4136)
                     /gene="rrtA"
                     /locus_tag="PL152_02495"
     CDS             complement(3504..4136)
                     /gene="rrtA"
                     /locus_tag="PL152_02495"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhombosortase"
                     /protein_id="HQR48175.1"
                     /translation="MAALNLRTTVLPRFPARRIANWLTAAGIAALLVVLGLAGPGTVS
                     VLRYHRSAVLSGQAWRLVTGHLVHADAAHLAWNLAGLALVWWLFAAEFTLGGWCLVML
                     ASTAAIDLGFLLFDPDIEWYVGFSGVLHGCMAAGLLAWLHRGRDWLVMVVAAVFAAKL
                     GWEHFAGPVPFTGGTLSVPVVVEAHTYGAVGGALAALWLLRRGPGRSAPL"
     gene            4181..4633
                     /gene="gspG"
                     /locus_tag="PL152_02500"
     CDS             4181..4633
                     /gene="gspG"
                     /locus_tag="PL152_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system major pseudopilin GspG"
                     /protein_id="HQR48176.1"
                     /translation="MNSRRIPRRHGGHTAGFTLIEIMVVVVILGILAALVAPNVIRRI
                     DDARVTKARQDIRAYETALNLYRMDNFRYPTTDQGLEALVKRPADPNLKNWKEGGYID
                     GLKKDPWGNDYTYLSPGSHGEYDLYTLGADGQPGGEGPNADIGNWNIE"
     assembly_gap    4736..4835
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4875..5441
                     /gene="gspH"
                     /locus_tag="PL152_02505"
     CDS             4875..5441
                     /gene="gspH"
                     /locus_tag="PL152_02505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspH"
                     /protein_id="HQR48177.1"
                     /translation="MTAHTADRNRALGAAAGFTLLEVLVVVVIIGIIASMAVVSVNVL
                     GGDREMDQETARLAAVLGQAREDAMLQGRDVGLRLEPAGYDFLRYDTRSEQWTVASDD
                     PMLRGRSLPAGVDLELRLESRDVPLASRASADPQVPPSPQIVVQASGDLVPFEILLRR
                     EGTDEVRRIAGTADGTITAGKIDDDTRG"
     gene            5422..5982
                     /gene="gspI"
                     /locus_tag="PL152_02510"
     CDS             5422..5982
                     /gene="gspI"
                     /locus_tag="PL152_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR01707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspI"
                     /protein_id="HQR48178.1"
                     /translation="MTTHAAESPRQASGHAAGFTLIEVLAALVIVALGMLGAIEAVTQ
                     NARNGTYLRDKTLAHWVAMNVITEQRLQQAAPPVAESSDDVEFAGQRWHWTLKVSQTQ
                     VQSMRRLDVTVRPADAPDSTALASVAGFYGTAISPTPTGTLPWSGVPGGDGGDGDSGQ
                     GAENDSGNKSGGNPRVKPPRPPSDDE"
     gene            5991..6599
                     /gene="gspJ"
                     /locus_tag="PL152_02515"
     CDS             5991..6599
                     /gene="gspJ"
                     /locus_tag="PL152_02515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspJ"
                     /protein_id="HQR48179.1"
                     /translation="MARAAGFTLLELLVAIAIFAIVAAMALTGYTQLQRQSEYADQRL
                     ERTRELQRAVQVISQDIEQIEPRPIREPLGEGTVAALSSGTGVDYAIEFTRAGWSNTA
                     GLQRPTLERVGYRVENDELWRDHWPVLDRTIATEPVRLKLLTNVKSISLRFMNPSRTW
                     SDRWPESEVAGGSDDRARPIAIEVTLELEDLGKIRRVLEVPG"
     gene            6596..7552
                     /gene="gspK"
                     /locus_tag="PL152_02520"
     CDS             6596..7552
                     /gene="gspK"
                     /locus_tag="PL152_02520"
                     /inference="COORDINATES: protein motif:HMM:NF037980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system minor pseudopilin GspK"
                     /protein_id="HQR48180.1"
                     /translation="MKRASRPPTRASQRGVALIMAVLMVALAMILAANVVFRGVLDQR
                     RAATMFALDQAFEVALGAEAWAADFLQRDLQESKTDNLAETWAQPLPPLPIEGGSVAG
                     RLEDLQGRFNLNNLVFADGTTNPDAVKQLERVLVTLDIEPKWASAMADWIDEDNQPGF
                     PDGAEDSVYTGQTTPYLAANMPITRTSELLALAGFGSERYAKLKPYVSALPVGTPLNV
                     CTAPGIVLDALTERTREFGLNPDDLAKRRVEGCFPTLADLRGALGDADYDKIKNSLSE
                     SSSYFGATVWVTIGTTDFTLYSLLVRNQGGLVRPQLRSFGTQ"
     gene            7568..8791
                     /gene="gspL"
                     /locus_tag="PL152_02525"
     CDS             7568..8791
                     /gene="gspL"
                     /locus_tag="PL152_02525"
                     /inference="COORDINATES: protein motif:HMM:TIGR01709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HQR48181.1"
                     /translation="MSETLVIRVRAAEGAPASWLIVDSNGARSGSVQTGSLADALPLA
                     AGRSVVLLVPGSEVTLAEPELPMRGGARLAQAVPYALEEQLASDVEGLHFAIGKRPAD
                     ATGTPVAVVARGLMDRWLATCDAAGIHPDSVYADSLAVPVAPGSCTLLLDDASLYVRH
                     ADATPFVLDATTLSDALDLTLEGTGEAIGSVTFYAAPADYERHRTTIENVRERATALQ
                     VKLLPDGALPLLASQSVHMAGIDLLQGPYAAKSSMAVGLRRWRVPAVLAAATALVFVL
                     VQAVSAWQLSRAEKKLDAEIAQVFSQAMPGQKFVDPRAQMQGLLGGGTGRSALLPAVS
                     ALATALAGMPQARVQSIACRGETVDVRLTVPSVASLESVKQSLGRGGLSAEIQSATPR
                     GATVDGRLVVKLGQS"
     gene            8788..9282
                     /locus_tag="PL152_02530"
     CDS             8788..9282
                     /locus_tag="PL152_02530"
                     /inference="COORDINATES: protein motif:HMM:NF016493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein M"
                     /protein_id="HQR48182.1"
                     /translation="MNVRQWHDSLAPRERNLVYVAAALVGVAVLYLGLALPLEHLHAR
                     QVARVEKKTADLAWMRQAAPRVAALAARGGAAGSESLVVLVDRTGRQAGLAGAVRDQS
                     PNGSNGLRLRLEGAPFDAMVSWLAGLQQQYGVNVSDATVDGGSAPGLVNASITLTHGA
                     AAAP"
     gene            9260..10078
                     /locus_tag="PL152_02535"
     CDS             9260..10078
                     /locus_tag="PL152_02535"
                     /inference="COORDINATES: protein motif:HMM:NF013376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein N"
                     /protein_id="HQR48183.1"
                     /translation="MAQRPRRDVTARPEASSSRRTTGWLVAVGLVALACFVIATLPAS
                     LLAGRVQPYQLGASGYSGTIWSGTANGFAWRGATLGDLQWRLRPWALLRGRIAADVAM
                     SLPGGTASAALSAGPSGQIALDDVNLDLPLEALSALPVASVRGWQGRAAGHFDTLSVA
                     AGSLQDARGTLDLTGLVSPAPNHTPYGSYRFVIPDPAVATTGAAPGVTAHMSDLGGSL
                     AVDGRVTFSAGRSYLLEGTVAPRGNAPESLQRALQFLGPADAAGRRPFSVSGTL"
     gene            complement(10075..13461)
                     /locus_tag="PL152_02540"
     CDS             complement(10075..13461)
                     /locus_tag="PL152_02540"
                     /inference="COORDINATES: protein motif:HMM:NF024642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HQR48184.1"
                     /translation="MTDLRRADELAREAALDVRRSFIVQAPAGSGKTELLIGRFLALL
                     ATVRSPESVLAITFTRKAAAEMRDRIVVALRAAADPDSAPRDMHPRTLELASAALAID
                     RQLDWDLLGNPGRLRIQTIDALNLGLARRLPVLSGLGSGIGVEEDGRELYRAAAERLL
                     EHLPAGDPLHSSAVATLLGHLDNRVPRFVDLVVEMLARRDSWGSVLPTLAGNDLEGAE
                     LRSRLEGARAGLASTHLEALLRDFPQALWPDVVAVAREAAVNLAAAGADSPIVAWQDR
                     RSAPGHDPGELPLWLGLATLLLTADGSPRKSFTINQGIPPGKAGAALKQRAKDIAGTL
                     VPHAELTARLHAVRDVPPAHYDPGEWHVLQAQLVVLRLALAELEVVFSERRVADYQRI
                     AEAARQALGSDEQPTDTALALDATLCHLLVDEFQDTSEVQVRLLERLTAGWQRGDGRT
                     LFLVGDPMQSIYRFRNAEVGLFLDVRRHGIGTVQLEPLTLAVNFRSTTPVVDWINHCF
                     ERVLPAGDDDVTGAVSYSPSTTAQHASGEGGVRLHPLFGRSRQHEAREVADIVERRLA
                     EDAEADVAILVQGRSHLLHIVGELVRRNVAFQATDIDPLGERPVVLDLLALTRALAHP
                     ADRPAWLAVLRAPWCGLTLADLHALCGDDRDSTIPELLGDAARRLRLSTDARARLDRA
                     LHVLEQAPVELRRFGLRDAVERAWLALGGPATATSPRELDEAQAYLDELADLEQRGRG
                     TADLTRLAAALEKLYAPSWPDPGIRVQLLTVHKAKGLQFDTVIVPGLERRTRGDDRRL
                     LQWARLPGSGGREVVLAPVAATGADPHPLYRWLERLEAAKLAQEKRRMLYVAATRARR
                     WLHLFGTSGVVQVDGEDPRLVAPASSCALGLLWPVVESGFQARLAEVGAISGEVAPGI
                     PRDPPLRRLPLAWRIPEPQPPRLVSSSKTRTTEQPALEFDWASETARHVGTVVHRELL
                     HLASDGGGRSRSEAAARRRWHDELAELGVPADRRTEAVERVVAAVQRTLADERGRWLL
                     DAAHGAAASEFAITGPIAGEVVSVVIDRTFVDAGGTRWIVDYKTSSHEGAGLEEFLDS
                     EQSRYRPQLERYAELVRRLGPEPVRIGLYFPLLSAWRSWA"
     gene            complement(13454..16105)
                     /locus_tag="PL152_02545"
     CDS             complement(13454..16105)
                     /locus_tag="PL152_02545"
                     /inference="COORDINATES: protein motif:HMM:TIGR03623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQR48185.1"
                     /translation="MAVGKDRLQPAVTTVVPTRRLAHWLRGRHDDDCLARGLEVWHTP
                     DIVPWSEFVARTYGMARRAARVEGRWLPDAAARLAWERIALDDPATASLVSPGLAGRT
                     AYQSWRTMHAYGIPPAALDEDDRPEALAFTRWMRRYTEWLSDRGWMDEPGALPALDSV
                     SVGAGLEVVGFDELTPAQEAYFQRVVAAGTRVEHVQPQPRRGSATWFQCRDRAAEIDT
                     ATRWAAQRLQREPQSRLAIVVPDLAATLDEARRAIDRVLAPGATLTGGPAPGSHGYQL
                     VAARALSAQPIVAAALEVIESFAHPVDLAIASRVLSNAFLAAARAESNARARLDALIR
                     RQEGPGLSLQRLAQLAGQHGCPQLARALRDGVALAREWPKRALSSHWAHLWSRTLTAV
                     GWPGPGLDSNEHQARERWQQLLGEIGATDDCVGPLTAAQAAALLRELADRTLFEPQEL
                     AAALTVIDPQTCAGMSFDALWICGLDAGRWPSPASPDPFLPREWQVRQRVPGATAELA
                     SLDARRLLSRLRASADEVILSVPGFDGDAPLQPSALLAGVPRGDVSGIWAAPSLARAT
                     FAARPEPERLVDGAMPPIARGATAQGGARLIETQSACPFRAQAEFRLGARALEDPELG
                     IAAAERGELVHRVLARIWSELGDQLSLRRLSLAARDTLIADAITAECASLPGTSDEVT
                     RHLLGIESDWLAARVKELLEADLGRPPFAVASLERDALTEVGGLTLRLRVDRVDRIED
                     GGLVVIDYKTGADLEPKAWLGERPRLPQLPLYAEAVGPGEVAALAFGRLRSGSTGYSG
                     MARDAEAFPGLAAPGAKSWPKEFPSWTALRAEWRRCLAALAAEHAAGVARLAPDPARA
                     CQYCPLGILCRIGEAAAGGAAEDAGDD"
     gene            complement(16148..17428)
                     /locus_tag="PL152_02550"
     CDS             complement(16148..17428)
                     /locus_tag="PL152_02550"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR48186.1"
                     /translation="MPIRTIRPSPGLANVRYEIRGRLARRAHEMERLGYDIVSLNIGN
                     PGAFGFRTPETMRLAMIENLRSAEGYCHQKGIFPAREAVVMQQQERGVRGVTAEEVFI
                     GNGVSELIDLTLRALLTSDEEVLVPSPDYPLWTAAVNLNRGRAVHYPCHPERGFVPDP
                     EEIESLVTKRTRAIVVINPNNPTGAVYPRAVLEAIARIAERHHLVVLADEIYDQVLYD
                     DAEFVPMATLVQNTLCGTLSGLSKVYRACGYRVGWAVFSGDVEHAREYLSALELLSSL
                     RLCSNVPGQWAVQTALGGYQSIRELVSPGGRLFESRRAIVDAVARSRFLYLTAPRGAM
                     YAFVGVRPDALADFDDQQFALDLLEHKHVLVAPGTSFNVPYRNHFRVTNLPEPQVLTE
                     VFGRMEELLGNYALGEQPASRPSPSLRVVDAAKR"
     gene            complement(17496..18722)
                     /locus_tag="PL152_02555"
     CDS             complement(17496..18722)
                     /locus_tag="PL152_02555"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HQR48187.1"
                     /translation="MAGPLDGIVVLDLSRILAGPWATQALADFGAEVIKVEHPRGGDD
                     TRKWGPPYARDPQGRETTESAYYLSANRGKKSVAIDLSRSEGQDLVRRLAAQADVLVE
                     NFKVGGLARYGLAYEDVSPLNPRLVYLSISAFGQDGPDAAEPGYDAMIQGMGGLMSLT
                     GVPDGEPGAGPQKVGVAVADLMCGMYAVAGVLAALHERHASGRGQYIDLSLLDTQVAW
                     LANQNLNYLVSGTPPQRRGTAHPNIVPYQAFATADGHFMLAVGNDAQFQRFCAVIGAP
                     QLATDPRYATNESRVAQRGELVPLIAEALRRGRSAEWLARLRAAGVPAGPINDLAQVF
                     AEPQVRYREMRIDLPHPAAGTVPGVRNPVRFSRTAIDYERAPPLLGGDTDEVLRDRLG
                     LEAGALAQLRAGGVVA"
     gene            complement(18766..>19475)
                     /locus_tag="PL152_02560"
     CDS             complement(18766..>19475)
                     /locus_tag="PL152_02560"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR48188.1"
                     /translation="DLGLLLLTAASIGFVHTLLGPDHYLPFVAMSAARRWTPRRTYWV
                     TALCGVGHVLGSVVIGVLGIGIGISLHRIQWFEGLRGDVAAWLLAGFGLAYMAWGLKK
                     AWRSRPHTHEHVHADGTLHRHEHGHEGGHLHPHVDPARARSITPWALFVIFVLGPCEP
                     LIPLLMYPAAKHSTWGLVAVVVAFGVVTIGTMLIVVGLAMKGIQRLPLDAAERYSHAL
                     AGAALSLCGLAIIFLGV"
BASE COUNT         2965 a         6919 c         6661 g         2830 t
ORIGIN      
        1 ggcaatccgc caccgacgct ttcgagcagg acgggataac gatccgggaa tgccgcatgc
       61 aacccgagca aggcggactc gggcaaggct gcccgaacgg tcactgcggg gcgcggaaga
      121 cgaggctgcc gttggtgccg ccgaacccga aggaattcga cagcgcggtc tttagcggca
      181 tgcgccgcgc gctgcccggc acgtagtcca ggtcgcacgc ggggtcggga ttctcgagat
      241 tgatcgtcgg cggcacgatt ccgtcgcgga tggcgaggat cgtgaagatc gcctccaccg
      301 cgccggctgc gccgagcagg tgtcccgtca tggacttggt ggagctgatg gcgatgcgcc
      361 cggcgtggtc gccgaatgcg cgcttgatcg cgaccgtctc ggcgcggtcg ccgagttccg
      421 tcgacgtcgc gtgcgcgttg atgtgctgca cgtccgccgg cgtgacgcct gcatcgcgga
      481 ctgcgttcgc catggcgaga tatgcaccct cgccgttctc gggcggggcc gtgatgtggt
      541 acgcgtcgcc gctcatgccg aagccggcga actccgcgta gatcgtggca ccccgcgccc
      601 gcgcatgctc gtactcctcg agcaccagcg cgccggcgcc ttcggccatc acgaacccgt
      661 cgcgatcgcg gtcccagggc cggcttgcgg cagcggggtc gtcgttgcgc gtggacagcg
      721 cgcgagcctg gcagaagctg ccgaggccga gcggcgtgaa tgccgcctcg gcgcctccgg
      781 caaccatcac gtcggcgtcc ccgtactgga tcgtgcgcgc cgcgatgccg atgcagtgag
      841 tcgacgtggt gcaggccgtc accatggcga ggttcgggcc cttcatgccg tagtggatcg
      901 agacctggcc cgccgccgcg ttgatgatgc tgcccgggat gaagaacggc gagatcttgc
      961 ggggcgtctt cgcctccagc cacttcgccg tgttctcctc gatcgtgctc aatccaccga
     1021 tgccggcccc gatcgcgacg ccgagcctcg gcgcattggc ctcggtgacg acgatgccgg
     1081 agtccttgac ggcctgcacg gccgccgcga acccgaaggg cacgaacgga tcgaacttgc
     1141 ggacgtcctt ggccggcatg tagtcctcgg cgttgaagtc gcgcacttca ccgccgatcc
     1201 gcacggggaa tgccgtcgtg tcgagtcgcg tcagtgcgac gatgccgctg cgtccattgc
     1261 agaccgcgtc ccacgccttg gcaacgctgc tgcccagcgg cgaaacgatg ccgaggccgg
     1321 tgacgacgac tctgcgcttg gacaagctgc gtctccccgt cggggcaggt gtggccccgg
     1381 ccgccctccg tggcggacgg gctggctcgc agctcaggcc tggctgcgac gctcgttgat
     1441 gtagtcgatc gcctgctgga ccgtggtgat cttctcggcg tcctcgtcag ggatctcggt
     1501 ctcgaactcc tcctcgagcg ccatcacgag ttcgaccgtg tcgagcgagt ccgcgccgag
     1561 gtcatccacg aaggacgaat cgttgttgac ctcttcttcc ttcaccccca gctgttcggc
     1621 aacgatcgcc ttgacccgct gttcgatggt gctcattgtc gttggagtcc tctgtgggaa
     1681 acatgccagc cggaccctcc cttcgggcgc ccggcatggg gcgcgtattc taagttaagg
     1741 gtcgagtggg gtccaattgg gaccaattcc tatttaaatc atgtacatac cgccgttcac
     1801 gtggagggtc tccccggtca cgtaaccggc ctggggcgat gccaggaagg ccaccgcgtg
     1861 cgccacgtcg tccggtgacc caaggcggcc gagcgggacc tgcgccagca gcgccttgcg
     1921 cgcctcgtcg ttcagggcgt cggtcatgtc cgtgacgatg aagccggggg cgacgacgtt
     1981 gaccgtgatg ccgcgcgacc cgacctcccg ggccaggctc ttgctgaacc cgatcatgcc
     2041 ggccttggcg gcggcgtagt tgcactggcc ggcattgccc atgaccgcga tcaccgaggc
     2101 gatgttgacg atgcggccgc gacgggcctt catcatcggg cgcatgaccg ccttgcaggt
     2161 gcggtacacg gccgtgaggt cggtatcgag cacggcctgc cagtcctcgg cgctcatgcg
     2221 catgaggaga ccgtccttcg tgatcccggc attgttgacc aggatcgtcg gcgcaccttc
     2281 cgccgcctcg acctccttga agcagctctc gaccgatgcg gcgctcgaca cgtcgagcac
     2341 gacgccccgc cccttgagac cggccgccgc aagggcgtcg ctgatcgcgg ctgcgccctg
     2401 ccccgtcgtc gccgtcccga cgacccgcgc gccgcgccgt ccgagttcca gggcgatcgc
     2461 gcgcccgatg ccgcgggatg cgcccgtgac gagcgccagt tcgttctcga gcatctgcta
     2521 cccctgcact gcggccagcc cggcggccag cgaatccgga tcgtcgatcg agagcacctt
     2581 gaggtcagcc cgccgctcga cacgcttgtt gagcgtcgtc agcaccttgc ccggcccgca
     2641 ttcgaccagg gtcgtcacgc cggatgccag catcgcgcgc accgtgtcgg cccaccgcac
     2701 cggtttgaac aactgctggc gcagcgccgc gcggatgccg tccgcgctcg tgtgacgctg
     2761 gacgtcgacc gtgtagacgt ccggcacgct cggcatcctc acctcgacgg tcgcgaggcg
     2821 gcccgcgagt tcgtccgccg ccccgatcat gaggctcgag tgcgatggca cactcacggg
     2881 cagcgcaacg gcgcgcttcg ccccgcgggc cttcgccgcc tcgatcgcgc gcgccacggc
     2941 cgtggcgtgc cccgcgacga cgacctgccc gggcgcattg aaattgacag gctcgacgac
     3001 ctcgctgccg gccgcttccg cgcacgcggc cgcgacgtcg gcatcgtcga gcccgagtat
     3061 cgcggccatt gcgcccgtgc cgagcggcac cgcccgctgc atgacctgcc cgcggaagcg
     3121 cacgagatcg accgccgtgc ggaagtcgag cgagtccgag cagacgagcg ccgtgtactc
     3181 gccgaggctg tggcccgcca tcgccacagg catcggcccg ccccgcccac gccacgctcg
     3241 ccacgttgcg acgccggcgg cgagcatcgc gggctgggtg cgctcggtga ccgcaaggtc
     3301 cgcctccggc ccgtcctggc acaacgccca caggtcgtag ccgagcacgc cggacgcttc
     3361 gtcgaacgtc tggcgcacga ccggctccgc cgcggcgagc gaggcgagca tccccacgga
     3421 ctgggaaccc tgtcccggaa acacgaatgc cagcgacatc actcccccaa ggctcgatcc
     3481 ggcgggtccg ccaggccggg cgattatagc ggcgcggacc gacccggccc gcgtcgcagc
     3541 agccagagtg ccgccaatgc gccgccgacg gcgccatatg tatgggcctc gacgacgacg
     3601 ggcaccgaca gcgtcccgcc cgtgaatggg accggcccgg cgaagtgttc ccagccgagt
     3661 ttcgcggcaa acaccgccgc cacgaccatg acgagccagt cgcgaccccg atggagccac
     3721 gccaggaggc cggccgccat gcaaccgtgc agcacgccgg agaaacccac gtaccactcg
     3781 atgtccggat cgaacaggag gaacccgagg tcgatcgccg cggtcgaagc cagcatgacc
     3841 aggcaccagc cgccgagcgt gaattcggcg gcgaacagcc accagacgag cgccagcccg
     3901 gcgaggttcc aggcgaggtg ggcagcgtcc gcgtgcacca ggtgcccggt gacgaggcgc
     3961 cacgcctggc ccgacagcac ggcgctccga tggtagcgca gcacggaaac cgtgccgggc
     4021 ccggccagcc cgagcacgac gagcagcgcg gcgatgcctg ccgccgtgag ccagttcgca
     4081 atgcgtcggg ccgggaagcg cgggagcacg gtcgtccgga ggttcaatgc cgccatttgc
     4141 tatgattccg cgactctaca gccacaggcg tccacacgcg atgaactccc gcaggatccc
     4201 acgccgacac ggcggtcaca ccgccggatt caccctcatc gaaatcatgg tcgtggtggt
     4261 catcctcggc atcctggcgg cgctggtcgc gccgaacgtg atccggcgca tcgacgacgc
     4321 ccgcgtcacc aaggcccggc aggacatccg cgcgtacgag acggcgctca acctctatcg
     4381 catggacaac ttccgctatc cgacgacaga ccagggactc gaggcgctgg tgaagcgccc
     4441 ggcggacccg aacctcaaga actggaaaga aggcggttac atcgacggac tcaagaagga
     4501 cccctggggc aacgactaca cctacctgtc cccgggcagc cacggcgaat atgacctgta
     4561 caccctcggt gcggacggtc agcccggcgg cgagggcccc aatgccgaca tcggcaactg
     4621 gaacatcgag taaccggacc gggcagccgg aagccgccgc aggtcgatga cggctcgtaa
     4681 ggctgatcgc aaccgaaccc tccgagccgc cgcaggtcga tgacagctca cacggnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggctg atcgcaaccg aaccctccga
     4861 gccgccgcag gtcgatgaca gctcacacgg ctgatcgcaa ccgcgccctc ggtgccgccg
     4921 ctggcttcac gctgctcgag gtactcgtcg tcgtcgtgat catcgggatc atcgccagca
     4981 tggccgtcgt ctcggtcaac gtgctcggcg gcgaccgcga gatggaccag gagaccgcgc
     5041 gactcgcggc cgtgctcgga caggcgcggg aagacgcgat gctgcagggt cgcgacgtcg
     5101 ggctgcggct ggaacctgct ggctacgact tcctgcgcta tgacacgcgc agcgagcaat
     5161 ggacggtggc gtcggacgat ccgatgctgc gcggccgctc cttgccggca ggggtcgacc
     5221 tcgaactccg gctcgaatcg cgagacgtgc cgctggcctc gcgcgcctcg gccgacccgc
     5281 aagtgccgcc atcgccgcaa atcgtcgtgc aggcgtcggg ggacctcgtg ccgttcgaga
     5341 tcctgctgcg gcgcgagggc accgacgagg tgcgccggat cgccggcacc gccgacggca
     5401 cgatcaccgc gggcaagatc gatgacgaca cacgcggctg agtctcccag gcaggcgtcg
     5461 gggcacgccg ctggtttcac gctcatcgag gtgctggctg cgctcgtgat cgtcgcgctg
     5521 gggatgctcg gcgcgatcga ggcggtgacg cagaacgcgc gcaatggcac gtacctgcgc
     5581 gacaagacgc tggcgcactg ggtcgccatg aacgtgatca cggagcagcg gctgcagcag
     5641 gctgcgccgc cggtggccga atcctccgac gacgtggaat tcgccggcca gcgctggcac
     5701 tggacgctca aggtgtccca gacacaggtg cagagcatgc ggcggctcga cgtgaccgtg
     5761 cgccccgccg acgcgccgga ctcgacggcg ctcgccagcg tcgctggttt ctacgggacc
     5821 gcgatcagtc cgacgccgac cggaacgctg ccttggagcg gagttccggg cggcgacggc
     5881 ggcgacggcg acagcggcca gggagccgag aacgactcgg gcaacaagtc gggcgggaat
     5941 ccgcgggtca agccgccgcg cccgccgtcg gacgacgaat gatgaatcga atggctcgcg
     6001 ccgccggctt cacgctgctg gaactgctgg tcgcaatcgc gatctttgcg atcgtcgcgg
     6061 cgatggcact caccggctat acgcagctgc aacggcagtc cgaatacgcg gaccagcgcc
     6121 tggagcggac ccgcgaactg caacgggccg tgcaggtgat ctcccaggac atcgagcaaa
     6181 tcgagccgcg gccgatccgc gagccactgg gcgaaggaac cgtggccgcg ctctcctccg
     6241 gcaccggcgt cgactacgcg atcgagttca cccgggcggg ttggagcaac accgcaggat
     6301 tgcagcggcc gacgctggaa cgggtcggct accgcgtcga gaacgacgag ctctggcgcg
     6361 accactggcc ggtgctcgac cgcaccatcg ccacggagcc cgtgcgcctg aagctgctga
     6421 cgaacgtgaa gtcgatctcc ctgcgcttca tgaaccccag ccgcacgtgg tccgaccgct
     6481 ggccggagtc cgaggtggcg ggcgggtcgg atgaccgcgc ccgcccgatc gcgatcgagg
     6541 tcacgctcga gctcgaggac ttgggcaaga tccgacgggt gctcgaggtg ccgggatgaa
     6601 gcgcgcgtcc cgcccaccca cgcgggcgtc ccagcgcggc gtcgcactga tcatggccgt
     6661 gctcatggtg gcgctcgcga tgatcctggc cgccaacgtc gtgttccgcg gcgtgctcga
     6721 ccagcgtcgc gcggcaacga tgttcgcgct cgaccaggcc ttcgaggtcg ccctcggtgc
     6781 cgaggcctgg gcggccgact tcctgcagcg ggacctgcag gagtccaaga ccgacaacct
     6841 cgccgagacg tgggcccagc cgctgccgcc actgccgatc gagggtggca gcgtcgcggg
     6901 ccggctcgag gacctgcagg ggcgcttcaa cctgaacaat ctcgtgttcg ccgacggtac
     6961 gacgaacccc gacgccgtca agcagctcga gcgcgtcctc gtcacgctcg acatcgaacc
     7021 caagtgggcc tcggcgatgg cggactggat cgacgaggac aaccagcccg ggtttcccga
     7081 cggcgccgag gacagcgtgt acacaggaca gaccacgccg tacctcgcgg cgaacatgcc
     7141 gatcacgcgc acgagcgaac tgctcgctct ggcgggattc ggcagcgagc gctacgcgaa
     7201 gctgaagcct tacgtgtcgg cgttgccggt cggcactccg ctcaacgtct gcaccgcgcc
     7261 gggcatcgtg ctcgacgcgc tcaccgagcg cacgcgtgag ttcggcctga accccgacga
     7321 cctcgccaag cgccgggtcg aaggctgctt cccgacgctc gcggacctgc gtggcgcgct
     7381 cggcgacgcc gactacgaca agatcaagaa ctcgctctcg gaatcgagca gttacttcgg
     7441 cgcgaccgta tgggtcacta ttggcactac ggatttcacc ctgtacagtc tgctcgtccg
     7501 caaccagggc ggcctcgtgc gcccccagtt gcgcagtttc ggcacccaat gatcctctcg
     7561 aggcccgatg tctgagacgc tggtgatccg cgtgcgcgcc gccgagggag cgccggcctc
     7621 gtggctgatc gtcgattcga acggcgcgcg ctccggctcc gtgcagaccg ggtcactggc
     7681 cgacgcgctc ccgctcgccg ccgggcgctc ggtggtcctg ctcgtcccgg gcagcgaggt
     7741 cacgctggcc gagccggaac tgcccatgcg gggcggcgcg cgactcgcgc aagccgtgcc
     7801 ctacgccctc gaggagcagc tcgcttccga cgtcgagggc ctgcacttcg cgatcggcaa
     7861 gcgccccgcg gacgcgacgg ggacgccggt cgcggtcgtc gctcgcggcc tcatggatcg
     7921 ctggctcgcc acctgcgatg cagccggcat ccatcccgat tccgtgtacg cggattcgct
     7981 ggccgtgccc gtggcgcccg ggagctgcac gctgctgctc gacgacgctt cgctgtacgt
     8041 gcgtcacgcc gacgcgaccc ccttcgtgct cgatgcgacc accctgtccg atgcgctcga
     8101 cctcacgctc gaaggcacgg gtgaggcgat cggcagcgtc acgttctacg cagcaccggc
     8161 agactacgaa cggcatcgca cgaccatcga gaacgtgcgc gagcgtgcga cggccctgca
     8221 ggtgaagctg ctgccggacg gtgcgctgcc gctgctcgcc tcacagtccg ttcacatggc
     8281 aggcatcgac ctcctgcaag gaccgtacgc ggccaagtcg tcgatggccg tgggcctgcg
     8341 ccgctggcgc gtgccggccg tgctggcggc cgccacggcg ctggtgttcg tgctggtgca
     8401 ggccgtgtcc gcgtggcagc tgtcccgcgc cgagaagaag ctcgacgcag agatcgcgca
     8461 ggtgttctcg caggcgatgc cggggcagaa attcgtggac ccgcgcgcac agatgcaggg
     8521 gctcctcgga ggcggaaccg ggcgcagcgc gctgctgcct gcggtctccg cactggcgac
     8581 cgcactcgcg ggcatgccgc aggccagggt gcagtccatt gcctgccgcg gcgagacggt
     8641 cgacgtgcgc ctcaccgtgc cgtcggtggc gtcgctggag tccgtgaagc agtcgctcgg
     8701 caggggcggc ctgagtgcgg agatccagtc ggcgacgccg cgcggcgcga cggtcgacgg
     8761 ccggctcgtc gtcaagctgg ggcagtcgtg aacgtccgcc agtggcacga ctccctcgca
     8821 ccccgggagc gcaacctcgt gtacgtcgcg gccgcgctcg tcggggtcgc cgtgctctat
     8881 ctcggcctgg cattgcctct cgagcacctg catgcgcggc aggttgcgcg cgtggagaag
     8941 aagacggccg acctggcctg gatgcgccag gcggcaccgc gcgttgccgc gctcgccgcg
     9001 cgcggcggag ccgccggcag cgaatcgctg gtggtgctcg tcgaccggac cgggcgccag
     9061 gccggactgg ccggcgccgt ccgcgaccag agcccgaacg gcagcaacgg cctgcgcctg
     9121 cggctcgagg gggctccctt cgatgccatg gtttcctggc tcgcggggct gcagcagcag
     9181 tatggcgtca acgtgtccga cgcgaccgtg gacggaggct ccgcgccggg actcgtcaac
     9241 gccagcatca cgctcaccca tggcgcagcg gccgcgccgt gacgtgacgg cgcgcccgga
     9301 ggcgtcctcc tcgcgcagga ccactggctg gctcgtcgca gtcggcctcg tcgcgctcgc
     9361 atgtttcgtg atcgcgacgc tgcccgcgag cctgctggcg ggtcgcgtgc agccctacca
     9421 gttgggggcg tccgggtact caggcacgat ctggtccggc accgccaacg ggtttgcgtg
     9481 gcgcggtgcc acgctcgggg acctgcagtg gcgcctgcgg ccgtgggcgc tcctgcgtgg
     9541 acgcatcgct gcggatgtcg cgatgtccct tccgggaggc actgcctcgg ctgcattgtc
     9601 tgccggcccg agcggacaaa tcgcactcga cgacgtgaac ctcgatctgc ccctcgaggc
     9661 gctttcggcc ctgcccgtcg cgtcggtacg cggctggcaa ggacgcgccg cgggacactt
     9721 cgacacgctt agcgtggccg cggggtcact gcaggacgcg cgcggcacgc tcgacctgac
     9781 gggactcgtc tcccctgccc caaaccatac tccgtacggc agctatcgat tcgtgattcc
     9841 ggatccagcg gtggccacga ccggcgcggc gcctggcgtc acggcgcaca tgtcggacct
     9901 gggcggttca ctcgcggtgg acggacgcgt cacattctcg gcgggtcgca gctatctcct
     9961 cgagggcacc gtcgcgccgc gtggaaacgc cccggagtcg ctgcagcgcg cgctgcagtt
    10021 cctcgggccc gccgacgccg ccggacgccg gccattcagc gtcagcggca cgctctaggc
    10081 ccagctcctc cacgccgaca acagcgggaa atagagcccg atgcgcaccg gctccggccc
    10141 gagccggcgc acgagttccg cgtagcgttc gagttgcggc cggtagcggg actgctcgct
    10201 gtcgaggaac tcctcgagcc cggctccctc gtggctgctc gtcttgtagt ccacgatcca
    10261 acgggtgccg ccggcatcca cgaacgtccg gtcgatgacg acggacacga cctcccccgc
    10321 gatcggtccc gtgatcgcga actcgctcgc cgccgcgccg tgggccgcgt cgagcaacca
    10381 tcggccgcgc tcgtcggcga gcgtgcgctg gacggccgcc actactcgct cgacagcttc
    10441 cgtacggcga tccgcgggca cgccgagctc ggcaagctcg tcgtgccagc gccggcgcgc
    10501 cgccgcctcc gacctcgagc gcccaccgcc gtcgctcgca agatgcagca attcccggtg
    10561 cacgaccgtg ccgacgtgtc gggccgtttc cgatgcccag tcgaactcga gtgccggctg
    10621 ctcggtcgtt cgcgtcttgc tcgacgacac gaggcgcggc ggctgcggct cggggatccg
    10681 ccaggccagc ggcagccggc gcagcggcgg gtcacgcgga atgccgggcg cgacctcgcc
    10741 agagatcgcg ccaacctccg cgagccgcgc ctggaaaccg gactcgacga cgggccacag
    10801 caggccgagg gcgcacgacg acgcgggtgc gacgagccgc ggatcctccc cgtcgacctg
    10861 cactacgccg ctggtcccga acaggtgcag ccagcgcctg gcgcgcgtcg ctgcgacgta
    10921 cagcatccgg cgcttctcct gcgcgagctt cgcggcttcg agtcgctcga gccagcggta
    10981 gagcggatgc ggatcggcac ccgtcgcggc gacgggcgca agcacgactt cgcggccgcc
    11041 ggacccaggc aggcgcgccc actgcagcag gcgcctgtcg tcgccgcgcg tccggcgctc
    11101 gagccccggc acgatgacgg tgtcgaactg cagtcccttg gccttgtgca cggtcagcaa
    11161 ctgcacgcgt atcccgggat ccggccacga cggcgcatac agcttctcga gtgcggcggc
    11221 cagccgcgtg aggtccgcgg taccgcgccc ccgctgctcc aggtccgcga gctcgtccag
    11281 gtaggcctgg gcttcgtcga gttcgcgcgg cgaagttgcg gttgcgggac ccccgagtgc
    11341 cagccacgcc cgttcgactg cgtcgcgcaa gccgaagcgg cgcagttcca cgggcgcctg
    11401 ttccagcaca tgcaacgccc tgtcgaggcg cgcgcgtgca tcggtcgaca atcgcagccg
    11461 tcgtgcggca tctccgagca gttccgggat cgtcgaatcg cgatcgtcgc cgcagagcgc
    11521 gtgcagatca gcgagcgtca ggccgcacca gggcgcgcgc aggacggcga gccacgccgg
    11581 ccggtcggcc gggtgcgcga gggcacgggt cagcgcgagc aggtccagga cgaccgggcg
    11641 ctcgccgagc gggtcgatgt cggtggcctg gaatgccacg tttctgcgga ccagttcgcc
    11701 cacgatgtgc agcaggtgac tacgcccctg gaccaggatc gcgacgtcgg cctcggcgtc
    11761 ctcggcgagc cggcgctcga cgatgtcggc gacttcgcgc gcctcgtgtt gccggctgcg
    11821 cccgaataac gggtgtaacc gcacgccgcc ctcgccggaa gcatgctgtg ccgtcgtgct
    11881 gggtgaatag ctcaccgcgc cggtgacgtc gtcgtcgccc gccggcaaga cgcgctcgaa
    11941 gcagtgattg atccagtcga ccaccggggt cgtggagcgg aaattcaccg ccagggtcag
    12001 cggctcgagt tgcaccgtgc cgatgccgtg ccggcggaca tcgaggaaca agccgacctc
    12061 ggcgttacgg aatcgataga tggactgcat cgggtcgccg acgaggaaca gcgttcggcc
    12121 gtcgccccgc tgccagcccg ccgtgagccg ctcgagcagc cgcacctgca cctcggaggt
    12181 gtcctggaac tcgtccacga gcaggtgaca caacgtagca tccagcgcga gcgccgtgtc
    12241 cgtcggctgt tcgtcgctgc ccagcgcctg cctcgccgcc tccgcgatgc gctggtagtc
    12301 ggcgacacgc cgctccgaga agacgacctc gagttccgcg agcgcgagcc gcagcaccac
    12361 cagttgcgct tgcaggacgt gccattcccc cgggtcgtaa tgcgccggcg gcacgtcgcg
    12421 cacggcatgc agccgggccg tgagctccgc gtgaggaaca agcgtcccgg caatgtcctt
    12481 cgcgcgctgc ttcaacgcgg ccccggcctt gccgggtgga atgccctgat tgatcgtgaa
    12541 actcttgcgc ggcgacccgt cggccgtgag cagcagcgtg gcgagcccga gccacagcgg
    12601 cagctcgccc gggtcgtgcc ccggcgccga acgacggtcc tgccacgcca cgatcggcga
    12661 atcggcaccc gcagcagcga ggttcaccgc cgcttcacgg gccacggcca cgacgtccgg
    12721 ccagagcgcc tgcggaaagt cccgcagcaa cgcctcgaga tgcgtggatg cgagtcccgc
    12781 gcgtgcgccc tccaggcgcg aacgcagttc cgcgccttcc aggtcgttgc ccgcgagcgt
    12841 gggcagcacg ctgccccacg agtcccggcg cgccagcatc tctaccacga ggtcgacgaa
    12901 ccgtgggacg cggttgtcga gatgtcccag caacgtggca acggcactgg aatgcagcgg
    12961 gtcgccggcg ggcaggtgct cgagcaaccg ctccgctgcc gctcggtaga gttcccgacc
    13021 atcctcctcg acgccgatgc cggaccccag gcccgaaagc acgggcaggc ggcgagcgag
    13081 ccccaggttg agcgcatcga tggtctggat gcgcagcctg cccgggttgc cgagcaggtc
    13141 ccagtcgagt tgccggtcaa tcgcgagcgc cgcacttgcg agttcgaggg tgcgcggatg
    13201 catgtcccgc ggcgcggagt cgggatcggc ggcagcgcgc aacgcgacca cgatgcgatc
    13261 gcgcatttcc gcggccgcct tgcgggtgaa cgtgatggcg agcaccgact cgggtgagcg
    13321 cacggtcgcg agcagcgcga ggaaccgccc gatgaggagt tcggtcttgc ccgagcctgc
    13381 cggggcctgc acgatgaaac tgcggcgcac gtcgagcgcc gcttcgcgcg ccagctcgtc
    13441 cgccctgcgc aggtcagtca tcgccggcgt cctcggctgc accccctgcc gctgcctcgc
    13501 cgatccggca caggatgccg agcgggcagt attggcacgc gcgagccgga tcgggcgcga
    13561 gccgtgcgac accggccgca tgctcggctg ccagcgccgc gagacaccga cgccactccg
    13621 cacgcagcgc ggtccacgac gggaattcct tcggccacga cttcgctccc ggtgccgcga
    13681 gccccgggaa cgcctcggca tcgcgcgcca tgccactgta tccggtgctt ccgctgcgca
    13741 gccggccgaa tgccaacgcg gcaacctcgc cgggcccgac cgcctcagca tagagtggca
    13801 gctgcggcaa tcgtggccgt tcgccgagcc acgccttcgg ctccaggtcc gcgccggtct
    13861 tgtagtcgat gaccaccagc ccgccgtcct cgatgcggtc gacccggtcc acgcgcaatc
    13921 gcagggtcaa gccgccgacc tcggtcagtg catcccgctc gagcgacgcc acggcgaacg
    13981 gcggtcgtcc caggtccgcc tcgagcagtt ccttcacgcg cgccgcgagc cagtccgact
    14041 cgatcccgag caagtggcgc gtgacttcgt cggacgtccc cgggaggctc gcgcactccg
    14101 cagtgatcgc atccgcgatc agcgtgtcgc gtgccgcaag cgacaggcgg cgcagcgaga
    14161 gctggtcgcc cagttcactc cagattctcg ccaggacacg gtgcaccagt tcgccgcgct
    14221 cggcggccgc gatcccgagt tcgggatcct cgagcgcgcg ggctcccagc cggaactcgg
    14281 cttgggcacg gaatgggcaa gccgactggg tctcgatcaa ccgcgcgcca ccttgcgccg
    14341 ttgcaccccg tgcgatcggg ggcatggctc cgtcgacgag ccgctccggt tccggacgcg
    14401 cggcgaacgt ggcccgcgcg agcgacgggg ccgcccagat cccggagaca tcgccgcgcg
    14461 gcacgccggc gagcaatgcg ctcggttgca ggggagcatc gccgtcgaag cccggcacgc
    14521 tcaggatcac ctcgtcggca ctcgcgcgaa gccgggagag caggcgcctg gcatccagcg
    14581 acgcgagttc ggcggtggca ccgggcactc gctggcgaac ctgccattcc cgcgggagga
    14641 acggatccgg cgacgcgggg gacggccacc gccccgcgtc gagaccgcag atccagagtg
    14701 cgtcaaaact catgccggcg caggtctgcg gatcgatgac cgtgagtgcc gcagccagtt
    14761 cctgcggctc gaacaacgtg cgatcggcga gctcccgcag caatgccgcg gcctgcgccg
    14821 cggtgagcgg accgacgcag tcgtcggtgg cgccgatttc accgaggagc tgctgccagc
    14881 gttcgcgagc ctggtgctcg ttgctgtcga gccctggccc cggccagccc acggcagtca
    14941 gcgtgcgcga ccacaggtgc gcccagtgac tggacaaggc acgcttcggc cactcgcgcg
    15001 cgagcgccac cccgtcgcgc aacgccctcg cgagctgcgg gcagccatgc tggccggcca
    15061 gctgggccag tcgctgcagc gacaggccgg gaccctcctg gcgacggatg agggcatcca
    15121 ggcgcgcgcg cgcattcgac tccgcacgcg cagccgccag gaacgcattg ctcagcacgc
    15181 ggcttgcgat cgcgaggtcg accgggtgag cgaacgactc gatgacctcc agcgccgccg
    15241 caacgattgg ttgcgcggac agtgcgcgcg cagcgaccag ctggtagccg tgcgaccccg
    15301 gcgccggacc gcccgtcagc gtggcgccag gtgcgagcac ccgatctatc gcgcgtcggg
    15361 cctcgtcgag cgtcgcggcg aggtccggca cgacgatcgc gaggcgagac tgcggctccc
    15421 gttgcaggcg ctgcgcggcc cagcgtgtgg cggtgtcgat ttccgccgcg cggtcgcggc
    15481 attgaaacca tgttgcggac ccgcggcgcg gctgcggctg cacatgctcg acacgcgtgc
    15541 ccgcagcgac gacgcgctgg aagtacgcct cctgggccgg cgtcagctcg tcgaagccga
    15601 cgacttccag gcccgcgccg acggacaccg agtcgagcgc cggcaacgcg cccggttcgt
    15661 ccatccagcc gcggtcactc aaccactcgg tgtacctgcg catccatcgc gtgaacgcga
    15721 gggcctcggg tcgatcgtct tcgtcgagcg cagccggcgg tatcccgtag gcatgcatcg
    15781 tccgccacga ctggtacgcc gtgcgtccgg caagccccgg cgacacgagc gatgcggtcg
    15841 ccggatcgtc gagggcaatg cgctcccagg cgagcctcgc cgcggcgtcg ggcagccagc
    15901 ggccttcgac acgtgcggcg cgccgtgcca tgccgtacgt gcgcgcgacg aactccgacc
    15961 acggcacgat gtccggcgtg tgccagacct cgaggccgcg cgccaggcag tcgtcgtcgt
    16021 gcctgcccct gagccagtgc gcgaggcgtc gtgtcgggac gacggtagtc accgctggct
    16081 gcagcctgtc cttgcctaca gccatgagtt tatattcaga tacttacgta tcttttctaa
    16141 tcctgcttca tcgcttggct gcgtccacga cccgcaacga ggggctcggc cggctcgccg
    16201 gctgctcccc cagcgcgtag tttccgagca actcctccat gcgtccgaag acctccgtga
    16261 gcacctgcgg ctccggcagg ttggtgacgc ggaagtggtt tcgatacggc acgttgaagc
    16321 tcgtgcctgg tgcgaccagc acgtgcttgt gctccagcag gtcgagcgcg aactgctggt
    16381 cgtcgaagtc cgccagtgca tcgggccgca cgccgacgaa cgcgtacatc gcacccctcg
    16441 gcgccgtgag gtacaggaag cggctgcgtg cgacggcgtc gacgatggcc cggcgcgact
    16501 cgaacaggcg cccgcccggg ctcaccagct cacggatact ctggtagcca ccgagcgcgg
    16561 tctgcacggc ccactgtccg ggcacgttgc tgcacaggcg cagcgacgag agcagttcca
    16621 gcgccgacag gtactccctc gcgtgctcca cgtcgccgga aaacacggcc cagcccacgc
    16681 ggtagccgca agcgcgatac accttcgaca gcccgctcag cgtgccgcac aaggtgtttt
    16741 gcacgagcgt cgccatgggc acgaactcgg cgtcgtcgta gaggacctgg tcgtagatct
    16801 cgtcggcgag cacgacgagg tgatgcctct ccgcgatccg tgcgattgcc tccaggacgg
    16861 cgcgcggata cacggcgccg gtggggttgt tcgggttgat caccacgatc gcgcgcgtgc
    16921 gcttggtcac gagcgactcg atttcctccg gatccggcac gaagccacgt tccgggtggc
    16981 atgggtagtg aacggcgcgt ccgcgattca ggttcactgc ggcggtccac agcggatagt
    17041 ccgggctcgg gaccagcact tcctcgtcgc tcgtgagcag cgcgcgcagc gtgaggtcga
    17101 tgagctcgct cacgccgttg ccgatgaaga cctcctcggc cgtgaccccg cgcacgcccc
    17161 gctcctgctg ctgcatcacc accgcctcgc gggccgggaa gatgcccttc tggtggcagt
    17221 agccctccgc cgaacgcagg ttctcgatca tggcgagccg catggtctcg ggggtgcgga
    17281 acccgaaggc gcccggattg ccgatgttca gcgagacgat gtcgtacccg aggcgctcca
    17341 tctcgtgggc gcgccgggcc agccggccac ggatctcgta gcggacgttg gccaggccgg
    17401 ggctgggacg gatggtgcgg atcggcatgg gcgagccctg ttagctgcaa tgcaccaatt
    17461 atggcccggc gcggcctgcc tgcacaggac gggcttcacg cgacgacgcc gcccgccctc
    17521 aactgcgcca gcgccccggc ctccagtccg aggcgatcgc gcaggacctc gtcggtgtcg
    17581 ccgccgagca gcggaggcgc ccgctcgtag tcgatcgcgg tgcgcgaaaa ccggaccggg
    17641 ttgcgtactc ccggcacggt gccggccgcc gggtgtggca ggtcgatgcg catctcgcgg
    17701 taccgtacct gtggctccgc gaacacctgt gcgagatcgt tgatcgggcc agcggggaca
    17761 cctgctgcac gaagccttgc cagccactcc gcgctgcgcc cgcggcggag tgcttccgca
    17821 atcagcggga cgagttcgcc gcgctgcgcg acgcggctct cgttggtcgc gtaccgcgga
    17881 tcggtcgcga gctgcggggc cccgatcact gcgcagaagc gctggaactg cgcgtcgttg
    17941 ccgaccgcga gcatgaagtg cccgtcggcc gtggcgaagg cctggtacgg gacgatgttc
    18001 ggatgcgccg tgccgcgtcg ctgcggcggc gtcccgctca cgaggtaatt gaggttctgg
    18061 ttggcgagcc acgccacctg cgtgtcgagc agcgacaggt cgatgtactg gccgcgaccc
    18121 gacgcgtgcc gctcgtgcag cgccgcgagg acgcccgcga cggcatacat cccgcacatc
    18181 aggtccgcga ccgcgacacc gaccttttgc ggccctgcgc cgggctcgcc gtccggcact
    18241 ccggtgagac tcatgagccc acccatgccc tggatcatcg cgtcgtagcc gggctcggcg
    18301 gcgtccggcc cgtcctgccc gaatgccgag atcgacaggt acacgagccg aggattcagc
    18361 ggcgagacgt cttcgtacgc aagcccgtaa cgcgcgagcc cgccgacctt gaagttctcg
    18421 accagcacgt cggcctgtgc ggcgaggcgg cgcacgaggt cctgaccttc gctgcgcgac
    18481 aggtcgattg cgacggactt cttgccccgg ttcgccgaca ggtagtaggc ggattccgtg
    18541 gtctcccgac cctgcgggtc acgcgcatac ggtggccccc acttgcgcgt gtcgtcgccg
    18601 ccgcgcggat gctccacctt gatgacctcg gcgccgaaat cggccagcgc ctgggtcgcc
    18661 caggggccgg cgaggatgcg actcaggtcg agaacgacga tgccgtcgag tgggccggcc
    18721 atgctttgct cggggcggtc gaccggacgg ccggggagcg cgggactaga cgccgaggaa
    18781 gatgatcgcg agaccgcaga ggctgagcgc agcccccgca agcgcgtgcg aatagcgctc
    18841 cgcggcgtcg agcggcaacc gctggatgcc cttcatcgca aggccgacga cgatgagcat
    18901 cgtgccgatc gtgaccacgc cgaacgccac gacgacggcc acgagccccc acgtgctgtg
    18961 cttggcggcc ggatacatga gcaacgggat gaggggttcg cacggtccga gcacgaagat
    19021 gacgaacagc gcccagggcg tgatgctccg cgcgcgcgcc gggtccacgt gtggatgaag
    19081 gtgaccgccc tcgtgcccat gctcatggcg gtgcagcgtg ccgtcggcat gcacgtgctc
    19141 gtgcgtatgc ggccgggatc gccaggcctt cttcaggccc caggccatgt acgcgaggcc
    19201 gaaacccgcg agcagccagg cggcgacgtc gccgcgcagg ccttcgaacc actggatccg
    19261 gtgcagggag atgccgatcc cgatgcccag gacgccgatg accaccgacc cgaggacgtg
    19321 ccccacgccg cagagcgcgg tcacccagta cgtgcgtcgc ggcgtccatc ggcgggcagc
    19381 cgacatcgcg acgaacggca ggtagtggtc ggggccgagc agcgtgtgca cgaagccgat
    19441 ggaagcggcg gtgagcagca gcagtccgag atcga
//
LOCUS       DAOMFD010000022        18805 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_570_length_18805_cov_33.766667, whole genome shotgun sequence.
ACCESSION   DAOMFD010000022 DAOMFD010000000
VERSION     DAOMFD010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18805
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_570_length_18805_cov_33.766667"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2545)
                     /locus_tag="PL152_02565"
     CDS             complement(<1..2545)
                     /locus_tag="PL152_02565"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR48189.1"
                     /translation="MASTAPERGEEQEGPDLLATQVVFGSGGRPAVGIDVLATKVALD
                     FQALASETGDNCVKRNLDALREAIDVDAICIALFDPEKTAIERVASATGLFAPFDPQV
                     MKGDALERIPYLANTLDHLRILELRDTRAPRRELATDAARFAELGIRSMLVCGFSLAG
                     RVHGFMALCSSQPRESWDANLHLLLKLVGGSFATGLERLRVARYLGRLEERNALSLPT
                     ANDGLWDFDVENNTVYFSPRWRKMLGFDEHDPHVSPDWRRLVHPDDMARVQAMIRDHI
                     AGKSKMFESVHRMRHCNGEWRWVASRAKARVDSEGRLKRLVGVEWDITERKLYEDALF
                     KEKESAQITLQSIGDGVITTDQHLIMEYLNPVAEELTGWRFEEAQGRHIDDIFRGFHE
                     ETCEPLENPLSVAIRRSRAIKSVRPTLLIKRDGNEMYIESTASPIRDGSGAVSGGVLV
                     FHDVSESRELNRKLSYHASHDILTGLVNRREFEARLERALRSARARETSYALCHLDVD
                     QFKIINDTCGHSAGDALLGQVGALLKTKIRWRDTLARLGGDEFGVLLESCSLDEALRM
                     ADQLRETICGYKFVWEERTFRLGCSIGVVPITGDSEDVAAVLSAADSACAAAKEAGRN
                     RVYSFQENDIDLMRRRREMQWAARINNALEESRFELYRMTIQPLQRAESGAHYELLLR
                     MKDETGKIVSPDNFINAAERYGITPAIDRWVIENALRWLVSEADERERLELCSINLSG
                     QSLGDDKFLPFVIDQFHRSGIDASKICFEITETAAIASFSQANRFIQALKELGCKFAL
                     DDFGTGLSSFGYLKHFPVDFLKIDGSFVKEILHDPIDREMVRSINEIGHL"
     gene            complement(2685..4454)
                     /locus_tag="PL152_02570"
     CDS             complement(2685..4454)
                     /locus_tag="PL152_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQR48190.1"
                     /translation="MAAATADFAPTLLDRLLRPGRDSGRDHLYDVLWLLGLGLLLIGT
                     GLGLRDPWPPDEPRFALVAQDMLRSGDWLFPRIGGDLYADKPPFFFWLVAVATAATGS
                     LRIGFLLPSLLAGLGTVLLAYDLLRRARGREAGLATGLVLLVTFQFVWEARQAQIDAT
                     LCFLTTLSLYGLLRHLCIAPARNWFLAGWAAAGLGVITKGVGFLPLLALVPFAVLVRR
                     RWRAAVPRIGALGAAGAVCLLIAIGLWFVPMMVASSAGGDLLAYRQEILFHQTVTRYA
                     DAWHHHAPVYYYFVEIIPLFWLPLIALVPWLWPRWRDALRGRDTLIAVLLAWVLLVVL
                     FFTSSTGKRTLYILPAVPAFAMAAGPWLPELLRARGPRRVAFVLACAIAVVAAAAALV
                     LAVQPLRAEHVATVYGIRPVLPLAALAACCGLVLAYFRVRDGWLAYAGVLGTVLAFVG
                     IVVYPRIDAARSGRAFMARVEQASVGVRELGLVGAKEQYLLQLRRPSVNFGHARTRDE
                     RAGEANDAAAWLAEDPSRALVMDKRTRQRCFRDASAVDLGKANQQHWFLVTGVPDARC
                     AAAGNPASIYYYVPPAASVKTGS"
     gene            complement(4455..5216)
                     /locus_tag="PL152_02575"
     CDS             complement(4455..5216)
                     /locus_tag="PL152_02575"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR48191.1"
                     /translation="MTAPQGTNSTPGLDVSVVIPVCNEQDNVEPLAREIHAALAGRYA
                     FETIFVDDGSTDATAQAVRDARGAGMPEIRLLRHSVRSGQSAAVATGVRDARAPWIAT
                     LDGDGQNDPADIPKLLDAARAAGSSRLRLVMGNRTTRRDTWLRRLSSRVANGVRGGLL
                     KDGTPDTGCGIKVFDRAVFMDMPRFNHMHRFMPALFQREGYEVVSVPVNHRERTRGTS
                     KYGLHNRLWVGIVDLFGVMWLIRRASRRVPTSEDT"
     gene            5300..6070
                     /locus_tag="PL152_02580"
     CDS             5300..6070
                     /locus_tag="PL152_02580"
                     /inference="COORDINATES: protein motif:HMM:NF015961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR48192.1"
                     /translation="MSELHDDTQHEEPRNTLYRKVKRGKRRLTPGRLFVYRIAVVVAY
                     CVGRFLWWSTRMLPPIGLERAREAVREHRSLIPAYWHQHMLYGVRALLDLQPEGLKLG
                     FLISPSVDGAAPSMLVQRIGGHVIRGSSTHTGARALRDYYETIVKQQISPAITPDGPK
                     GPVHEFKPGAVLLAQLTGKPILLVSVAASRTWRFRTWDRFELPLPFSRVQIAYGEPVR
                     VPRVLNADGIARLQLEMADKLQALNAAAHAALRSGRPR"
     gene            complement(6047..6673)
                     /locus_tag="PL152_02585"
     CDS             complement(6047..6673)
                     /locus_tag="PL152_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48193.1"
                     /translation="MAHVLVLHGRLPPGPVDEAYAPLLARLPYAKRHELERRDPHARR
                     ASLAGIGLALHGIALLSGSPAEPRGLMFPTGGKPYVPGGPRFSISHGEHHVGVALSRD
                     CEVGFDLEEVQEPDTAPGATRETQAAQQRLVRWTATEAVLKAAARGLRAVGAVELDGN
                     YAAGRIGASIFRLAPVGIAGTVAHIATDVPITSLSVQEVPLSAWPAGS"
     gene            6757..8718
                     /locus_tag="PL152_02590"
     CDS             6757..8718
                     /locus_tag="PL152_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HQR48194.1"
                     /translation="MIKFETLPKALDDRARGTVTIGYLEGENAEKSLRLAELRRRALG
                     ILRHLQDMGARQGDTLILHVASNEQFIDAFWACLYGGIVPVPVAVGISDEHKHKLLRI
                     ARQLGTPWLYTDRKLRERLATFAASLEAASLWRDLERRSFLVDQLDDVSREGAPARLS
                     RDDTAFIQFSSGSTSEPKGVVLTHGNVLANAEGTRQAARLDEQDVSLSWMPLTHDMGL
                     IGFHLMMVYVGIRQYLMPTDLFVRRALLWPKFLSAKRATVTSSPNFGYRHFLRALGDK
                     QLEGVDLSGVRLIFNGAEPISVDLAHEFLDRMRGYGLRPTSMFTVYGLAEASLAVSFP
                     PPEAPMRHINVDRRSLGVGATARLVAATDPTAVKLACEGKPIPYCSVRLLDDAGNEVG
                     PDQTGHLLIRGDNVTRGYFENPEANAAAVSPDGWVRTGDLGLWHDGELYVTGRSKEII
                     FVNGQNYYPHDLEMILQQEPGLEHGKVAAAGVRTAGGATDDLVLFVVHRGEMADFVPT
                     ATKAMHLVNEHAGVEVARVVPIARMPKTTSGKLQRAALAESYANGEFAAELAAFDAAW
                     AAVHAQGRAAAGGIERQLKAIVDDVLAGKHVDVDDNLFEIGASSLTLIEIHEKIDELY
                     PGVVDLTELFDFPTVSQLAKHLEAKLGQG"
     gene            8735..9355
                     /locus_tag="PL152_02595"
     CDS             8735..9355
                     /locus_tag="PL152_02595"
                     /inference="COORDINATES: protein motif:HMM:NF024734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HQR48195.1"
                     /translation="MTLETQAAHILHIAGQRGVVRSHEVTEAGLARATLMRLVRDGQL
                     VQLARGVYALPGQRLSRRHPLAEVAARSSRGVFCLLTALHFHGLIPRPPDAVWLAIPN
                     KGRVPTGGNVPLEVVRFSGAALAEGVEHHLVDRVGIRVYAPGKTVADCFKFRGRVGLD
                     TAVTALRTVLRRRLATRAELEHYAGVCRVRETMLPYLARGAVQARE"
     gene            complement(9356..10192)
                     /locus_tag="PL152_02600"
     CDS             complement(9356..10192)
                     /locus_tag="PL152_02600"
                     /inference="COORDINATES: protein motif:HMM:NF020227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR48196.1"
                     /translation="MQKTIFITGCSSGIGAALAREFARRGHRVYATARRKDALAPLEP
                     LGIRGLQVDVDDDATIAAAFDTVMSEAGQLDLLVNNAGFSKVGAVIDLTRDDLRRQYE
                     TNVISPARVTAMAMPLLRSAVARHGSATVANVGSIVGLFTTPFAAAYCSSKAAVHSIT
                     DALRMELKPFGIHVVAIQPGGVRSSFGDHAEAAVRLPADSLYRAAEAGIHARAQAGQQ
                     GAMPADEFAAPVVDALLRSPPPRVVRRGTGSLRYPLLKRWLPGAMFDAKLAKVFGLDA
                     LR"
     gene            complement(10289..11128)
                     /locus_tag="PL152_02605"
     CDS             complement(10289..11128)
                     /locus_tag="PL152_02605"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR48197.1"
                     /translation="MTDGWRLRCCYAIGAVAAWLGYRVFGLRRSVIRDNLQRSFPDWP
                     GDRVRRVAREFAARQGELVAESLYATALGEAELRARVTIANPEVLGGTGSSRPLVLVG
                     AHHGNFEWMLYRLSLEFPGRFVGLYKPARNPRVDAWLKDRRGRFGARMVPAKSVLREL
                     AAIRDVGAIGLIADQVPRTSPEKHWVEFLGQDTAFYMGPELLGRALRSRVVLVRMRRL
                     ERGRYVLDYLPLNEPGEKLPGGEVTTRYARCLEDWIRADPPGWWWSHRRWKLKRKVYS
                     GGT"
     gene            complement(11121..11966)
                     /locus_tag="PL152_02610"
     CDS             complement(11121..11966)
                     /locus_tag="PL152_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR48198.1"
                     /translation="MLRFLALLPLPVLYAVCGALAFVTRVVGWRRRFVRDGIARCLPD
                     APQPERRRVARDFYRYLGELVAEAVHGTRIEASDLVDRLRFEDDDVVRDLLRDGKKVL
                     LLASHHCNWEWLLLRCSTAFGVPLTAAYKPASRERADRELGAMRSRFGATMVPAKQVV
                     NHLLAQRGAVRLLALVADQSPAAGREQQHWVRFFGTETDFFAGPGWISARLGFQPVYI
                     RMRREGRGRYVARFVPLLQAGERAHPERALEAYVRAVEDAVRSQPAQYFWAYNRWKRA
                     RRMYD"
     gene            complement(11975..12050)
                     /locus_tag="PL152_02615"
     tRNA            complement(11975..12050)
                     /locus_tag="PL152_02615"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12015..12017),aa:Thr,seq:cgt)
     gene            complement(12116..12547)
                     /locus_tag="PL152_02620"
     CDS             complement(12116..12547)
                     /locus_tag="PL152_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HQR48199.1"
                     /translation="MSHESTSAAGATARAKSILARHGVRPTAQRLRIAELLLTEPRHL
                     TAEQILETLRQSAGRVSKATVYNTLKLFVEAGLLHPIHTDPERCVYDSLTEPHYHFQD
                     LATGEMLDIPPQDISFAKMPALPPGTEVAGVDVVIRLRRTA"
     gene            12674..13204
                     /locus_tag="PL152_02625"
     CDS             12674..13204
                     /locus_tag="PL152_02625"
                     /inference="COORDINATES: protein motif:HMM:NF023444.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015627 - type II protein secretion
                     system complex [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspH/FimT family pseudopilin"
                     /protein_id="HQR48200.1"
                     /translation="MERPSGFTLPELMFSMAIVAGLVGWSVPAFRDIRLNAARTSEVN
                     LFLQAVYLARSEAIKRNGVVSLCPTLDGTACAPPGSTWDRGWIVFANRDRDSPAVRDA
                     GEELLSAYPAWEGGSIVANRGTLSFRAFGQSGVTATVTFCDSRGSGHARAVIISQAGR
                     PRVADRNSSGAALSCT"
     gene            13206..13685
                     /gene="pilV"
                     /locus_tag="PL152_02630"
     CDS             13206..13685
                     /gene="pilV"
                     /locus_tag="PL152_02630"
                     /inference="COORDINATES: protein motif:HMM:TIGR02523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus modification protein PilV"
                     /protein_id="HQR48201.1"
                     /translation="MDAAFRKHGGAQGGFSLVEILVVAAIVAVGLLGGEALVVTSLEA
                     SRSAMLQTIAVDLAADLGDRIRANPSAGSAYGLSAGARLGPPPKACRTVGECNSADLA
                     AVDLYLWQQATFTALPEATTVVQVEPAGGATHRYTIVLRWTATGRPAESRVAATVEI"
     gene            13682..14680
                     /locus_tag="PL152_02635"
     CDS             13682..14680
                     /locus_tag="PL152_02635"
                     /inference="COORDINATES: protein motif:HMM:NF027400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilW family protein"
                     /protein_id="HQR48202.1"
                     /translation="MSVAKTLAARQHTSCVGAGLVELMVALVIGAFLLSASATAYLGA
                     RNARAQLDAGARLQEIARYAMALIEADVRMAGYWGLTNRADDIAAHPSLAFPAKCGGK
                     PWVTSATQFVAGTNNAYLAVPSCAALSGGAQPGSDVLVVRRASAQRIAPQKATVGSTD
                     QDRVLVVSSHASGTVFVPQDLGNQIPASYATTDVAGLAPQADTRALVVDAYYVSRGSS
                     TAADFPALRRKTLTAGPDIGDEEVIAGVEDLQFQVGVDTDADPGADVYLDPGMLPTGA
                     RIVSVRIWLRLCAPDRGTALATQRIGPYGDRAAGIVTDTRLRLLVSRTVYLRNSGS"
     gene            14677..15243
                     /locus_tag="PL152_02640"
     CDS             14677..15243
                     /locus_tag="PL152_02640"
                     /inference="COORDINATES: protein motif:HMM:NF025699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilX N-terminal domain-containing pilus assembly
                     protein"
                     /protein_id="HQR48203.1"
                     /translation="MNRLRCVNRQHGVVLVVAMLLLLVLTLLGTTGLVTAVLELRMAA
                     NARYQARAFQAAEYGIQQAVHATGLATTITLASPRLEPAAGAPLRVPGTPQDSYSYRL
                     YYDTSTPPPPLVDGTVPGPGLRAYHFVAEATGRSERGATETHVQGFYLIASDPPPASL
                     TPPACAPGCSEAALYEPHRTFWMQVNAE"
     gene            15245..15742
                     /locus_tag="PL152_02645"
     CDS             15245..15742
                     /locus_tag="PL152_02645"
                     /inference="COORDINATES: protein motif:HMM:NF028042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilin protein"
                     /protein_id="HQR48204.1"
                     /translation="MRTRTSLWRARRHAPARASGGGFSLLDVVAAMAIVALLACIALP
                     SYRAQMMRANRAAARAALMALAAEQEKFYVECHAYATSLDGSAASTCGTSTLGFPASA
                     GDGTYRLRITEGDASGWTAVAEVVPGSRQEDDRRCRALQIDEVGHRTATTASGDASDA
                     ECWSR"
     gene            complement(15766..16719)
                     /gene="ispH"
                     /locus_tag="PL152_02650"
                     /gene_synonym="lytB"
     CDS             complement(15766..16719)
                     /gene="ispH"
                     /locus_tag="PL152_02650"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HQR48205.1"
                     /translation="MRILLANPRGFCAGVDRAIDIVERAIELFGAPIHVRHEVVHNRF
                     VVDRLRGLGAIFVDELDEVPDGATVIFSAHGVSKAVQDEARRRGLRVFDATCPLVTKV
                     HMEVARFARDGREVILIGHAGHPEVEGTMGQFDSTMGGRISLVEDATDVERLEVGDPA
                     RVAVVTQTTLSVDDTAEVLDALKQRFPTLTTPRKEDICYATQNRQDAVKSLVASCDVV
                     IVVGSRTSSNSNRLREIAEKAGIPGYLVDGPEDLEREWFEGRATVGVTAGASAPEVLV
                     QRVVARLREWGGQAADEVTGREENVVFALPRELRAARGTDS"
     gene            complement(16716..17192)
                     /gene="lspA"
                     /locus_tag="PL152_02655"
     CDS             complement(16716..17192)
                     /gene="lspA"
                     /locus_tag="PL152_02655"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019486629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HQR48206.1"
                     /translation="MQRTHPAAWLWLSLAVIGLDQATKFLVLRFFELYERVEVLPVLD
                     FTLLHNTGAAFSMLAGASGWQRPFFIALGIVVSVLLVAWIWRTPRGDKLLPFALSLIL
                     GGALGNVIDRVRLGYVVDFIHAHWGDAYFPAFNIADSAITIGAMLLIVDAFRGRRA"
     gene            complement(17244..>18805)
                     /locus_tag="PL152_02660"
     CDS             complement(17244..>18805)
                     /locus_tag="PL152_02660"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQR48207.1"
                     /translation="TPQWFISMEQAGLRAKALEEIGKVEWTPDWGEQRIYGMIAGRPD
                     WCISRQRTWGVPLALFTHRESGELHPDTPALVERVADLVEKDGIDVWFDLDPAALLGA
                     QAEHYEKVTDIMDVWVDSGVSHHCVPRLFPEVRTPADLYLEGSDQHRGWFHSSLLTSV
                     AQKGHAPYMGVLTHGFTVDEKGRKMSKSLGNTILPQKVVSTLGADVLRLWVASTDYAN
                     EIAVSDEILKRMSESYRRIRNTVRFLLGNMAGFDPAADAVPTSELVALDRWALARTRE
                     LQQEVVDAYRSYDFHLIYQKVHNFCVVDLGGFYLDVIKDRLYTTPAKGLPRRSAQTAM
                     YWIAEAMVRWLSPILSFTAEEIWRHMPGARAESVFHATWADLPAPAAEARAIDWPTVL
                     DVRSSVLKELERLRAAGEIGAPLDAIVEVYADAATSTALDAIGDELRFVFITSEAHVH
                     PADSRPTSAVPAGPADAPTLWLHVHASTATKCVRCWHKRNDVGSVAAHPELCARCASN
                     VDGPGEIRRFA"
BASE COUNT         2964 a         6664 c         6378 g         2799 t
ORIGIN      
        1 tcaggtgccc gatctcgttg atcgagcgca ccatctcgcg gtcgatcggg tcgtggagga
       61 tctccttcac gaagctgccg tcgatcttga ggaaatccac cgggaagtgc ttcaggtagc
      121 cgaacgacga caggcccgtg ccgaagtcgt cgagcgcgaa cttgcagccg agctccttga
      181 gcgcctggat gaagcggttc gcctgcgaga aactggcgat cgccgcggtc tcggtgatct
      241 cgaagcagat cttcgacgcg tcgatgccgc tcctgtggaa ctggtcgatc acgaacggca
      301 ggaacttgtc gtcgccgagg ctctggcccg acaggttgat cgagcacagc tcgagccgct
      361 cgcgctcgtc cgcttccgac acgagccagc gcagcgcgtt ctcgatcacc cagcggtcga
      421 tcgccggcgt gatgccgtag cgctccgcgg cgttgatgaa gttgtccggc gagacgatct
      481 tgccggtctc gtccttcatg cgcagcagca gctcgtagtg cgcgccggac tcggcccgct
      541 gcagcggctg gatcgtcatg cggtacagct cgaagcgcga ctcctcgagc gcgttgttga
      601 tgcgcgcggc ccactgcatc tcgcggcggc ggcgcatcag gtcgatgtcg ttctcctgga
      661 agctgtagac gcggttgcgg cccgcctcct tcgcggccgc gcaggcgctg tcggcggccg
      721 acagcacggc cgccacgtcc tcgctgtcgc cggtgatcgg cacgacgccg atgctgcagc
      781 cgaggcggaa ggtgcgctcc tcccacacga acttgtagcc gcagatggtc tcgcgcagct
      841 ggtccgccat gcgcagcgcc tcgtccagcg agcagctctc gagcagcacg ccgaattcgt
      901 cgcccccgag gcgcgcgagc gtgtcgcgcc agcggatctt ggtcttgagc agcgcgccca
      961 cctgccccag cagtgcgtcg ccggcgctgt gcccgcaggt gtcgttgatg atcttgaact
     1021 ggtccacgtc gaggtggcag agcgcgtagg aggtctcgcg cgcccgcgcg ctgcgcagcg
     1081 cccgctccag gcgggcctcg aattcgcgac ggttcaccag ccccgtcagg atgtcgtggc
     1141 tcgcgtgata ggagagcttg cggttcagct cgcggctctc gctcacgtcg tggaacacga
     1201 gcacgccgcc cgacaccgcg cccgagccgt cgcggatcgg cgaggccgtg ctctcgatgt
     1261 acatctcgtt gccgtcgcgc ttgatcagca gcgtcgggcg caccgacttg atcgcacggc
     1321 tgcggcggat cgccaccgac agcgggttct cgagcggctc gcaggtctcc tcgtggaagc
     1381 cgcggaagat gtcgtcgatg tgccggccct gcgcctcctc gaagcgccag ccggtcagct
     1441 cctcggcgac cggattcagg tactccatga tcaggtgctg gtcggtggtg atcacgccgt
     1501 cgccgatcga ttgcagcgtg atctgcgcgc tctccttctc cttgaagagc gcgtcctcgt
     1561 agagcttgcg ctccgtgatg tcccattcga ccccgaccag gcgcttcagc cgtccctcgg
     1621 agtcgacgcg agccttggcg cggctcgcca cccagcgcca ctcgccgttg cagtggcgca
     1681 tgcggtgcac gctctcgaac atcttggact tgcccgcgat gtggtcgcgg atcatcgcct
     1741 gcacgcgggc catgtcgtcg ggatgcacca gccgccgcca gtccggcgag acgtgcgggt
     1801 cgtgctcgtc gaagccgagc atcttgcgcc agcgcggcga gaagtagacc gtgttgttct
     1861 cgacgtcgaa gtcccacagg ccgtcgttcg cggtcggcag ggacagcgcg ttgcgttcct
     1921 cgagacgccc gaggtagcgc gccacgcgca gccgctcgag accggtcgcg aagctccccc
     1981 cgaccagctt gagcagcagg tgaaggttgg cgtcccacga ttcgcgtggc tgcgacgagc
     2041 acagcgccat gaacccgtgc acccgtcctg ccagcgagaa tccgcacacc agcatcgagc
     2101 ggatcccgag ctcggcgaag cgcgccgcgt cggtggcgag ctcgcggcgt ggcgcgcgcg
     2161 tgtcgcgcag ctccaggatg cgcaggtgat cgagggtatt ggcgagatac ggaatgcgct
     2221 cgagcgcatc gcccttcatg acctgcggat cgaacggcgc gaacagtccc gtcgcgctcg
     2281 cgacgcgctc gatcgcggtc ttctcgggat cgaacagcgc gatgcagatg gcgtcgacgt
     2341 cgatcgcctc gcgcaatgcg tcgaggttgc gcttgacgca gttgtcgccc gtctcgctcg
     2401 cgagcgcctg gaaatcgagc gccaccttgg tggcgaggac gtcgatgccg accgcgggtc
     2461 ggccaccgct accgaagacg acttgcgtgg cgaggaggtc cgggccttcc tgttcttcgc
     2521 cgcgctcggg tgcggtgctg gccatcgcgt tgtctcggac cccctgtcgg atgcagccag
     2581 tgtgtcaggc ctgcctcggc tgcagcggaa ctgttgctca attcagtttt ccgccgccgc
     2641 aattagttgc ggcgcattct gacacaggtt atcaggaatg caagctaact ccctgttttt
     2701 acagaagcgg ctggcggcac gtagtagtag atgctggcag gattgcccgc ggctgcgcag
     2761 cgtgcatccg gcacgcccgt gacgagaaac cagtgctgct ggttcgcctt gccgagatcc
     2821 acggcgctcg cgtccctgaa acaccgctgg cgcgtgcgct tgtccatcac gagcgcccgc
     2881 gacggatcct cggcgagcca cgcggcggca tcgtttgcct cccccgcccg ctcgtcacgc
     2941 gtgcgcgcgt gaccgaagtt gacgctcggc cgccgcagtt gcagcaggta ctgctccttc
     3001 gcgccgacga ggcccagttc gcgaaccccg acgctcgcct gctccacgcg cgccatgaag
     3061 gcgcggccgg accgcgccgc gtcgatccgc ggatacacga cgatgccgac gaaggcgagc
     3121 acggtcccga gcactcccgc ataggcgagc cagccgtcgc ggacacggaa gtaagcgagc
     3181 acgaggccac agcaagccgc caatgcggcg agcggcagca ccggccgtat cccgtacacg
     3241 gtcgcgacgt gctcggcgcg cagcggctgg accgcgagca ccagtgccgc tgccgcagcc
     3301 accaccgcga tcgcgcacgc cagcacgaat gcgacgcgac gcgggccgcg cgcgcgcagc
     3361 aactccggca gccacgggcc ggccgccatc gcgaaggccg gcaccgccgg caggatgtac
     3421 agcgttcgct tgccggtgct cgaggtgaag aacagcacca ccagcagcac ccacgcgagc
     3481 agcacggcaa tcagcgtgtc gcgcccgcgc agcgcgtcgc gccagcgggg ccagagccac
     3541 ggcacgagcg cgatgagcgg cagccagaag agcgggatga tctcgacgaa gtagtaataa
     3601 acgggcgcgt ggtggtgcca ggcatccgcg taccgggtca cggtctggtg aaacaggatc
     3661 tcctggcgat acgcgagcag gtcgccgccc gcggatgacg cgaccatcat cggcacgaac
     3721 cacaggccga tcgcgatcag caggcacacc gcgccggccg cgccgagcgc cccgatgcgt
     3781 ggcacggccg cacgccagcg ccgccgcacg aggaccgcaa atggaaccag cgcgagcagc
     3841 ggcaggaatc ccacgccttt ggtgatgacg ccgaggcccg ccgcggccca gccggccagg
     3901 aaccagttgc gcgcgggcgc aatgcacagg tggcgcaaca gtccgtacag cgacagcgtc
     3961 gtcaggaagc acaaggtcgc gtcgatctgc gcctgtcgcg cctcccacac gaactggaac
     4021 gtgacgagca gcaccagtcc ggtcgcgagg cccgcctcgc gcccgcgggc gcgacgcagc
     4081 aggtcgtagg ccagcagcac ggtgccgaga cccgcgagca gcgacggcag caggaagccg
     4141 atgcgcagcg agccggtcgc cgccgtcgcg acggcgacga gccagaagaa gaacggcggc
     4201 ttgtccgcgt agaggtcgcc accgatccgc gggaacagcc agtcgccgga gcgcagcatg
     4261 tcctgcgcca cgagcgcgaa gcgcggctcg tccggcggcc aggggtcacg cagcccgagc
     4321 ccggtgccga tcagcagcag cccgagcccg agcaaccaca ggacgtcgta gagatgatcg
     4381 cgcccggaat cccgtccggg cctgagcagg cgatccagga gcgtcggcgc gaagtcggcc
     4441 gtggctgccg ccactcaggt gtcctcgctc gtcgggacgc ggcgcgacgc gcggcggatc
     4501 agccacatca cgccgaagag atcgacgatg ccgacccaga gccggttgtg caggccgtac
     4561 ttcgacgtgc cgcgcgtccg ctcccggtga ttgacgggca cggacacgac ttcgtagccc
     4621 tcgcgctgga acagcgccgg catgaagcga tgcatgtggt tgaagcgcgg catgtccatg
     4681 aacaccgcgc gatcgaacac cttgatgccg cagccggtgt ccggcgtgcc gtccttgagc
     4741 agcccgccgc gcacgccgtt ggcgacacgc gacgacaggc gccggagcca ggtgtcgcgc
     4801 cgggtggtgc gattgcccat cacgaggcgc aggcgcgacg agcctgccgc acgcgccgca
     4861 tccagcagct tcgggatgtc ggccgggtcg ttctggccgt cgccgtcgag cgtcgcgatc
     4921 cagggggcac gcgcgtcgcg cacgccggtc gccacggccg cgctctgccc cgaccggacc
     4981 gagtggcgga gcaggcggat ctcgggcatg ccggcgccgc gggcatcacg caccgcctgg
     5041 gccgtcgcgt cggtgctgcc gtcgtccacg aagatcgtct cgaaggcgta gcgcccggca
     5101 agcgcggcgt ggatctcgcg ggccaggggt tcgacgttgt cctgctcgtt gcagaccggg
     5161 atgacgaccg acacgtcgag tcccggcgtc gaattcgtgc cctggggtgc ggtcaatgtg
     5221 cggactccgc ggagcgggac gctagtatag tcgcttcggg tcggcgcgcg agatcgtgga
     5281 ccgcacccga ggatattcga tgagcgaact gcacgacgac acgcagcacg aggagcctcg
     5341 caatacgctc taccgcaagg tgaagcgcgg caagcgcagg ctgaccccgg gtcgcctgtt
     5401 cgtgtatcgc atcgcggtgg tcgtcgccta ctgcgtggga cgtttcctgt ggtggtccac
     5461 ccgcatgctg ccgccgatcg ggctcgagcg cgcgcgggag gccgtgcgcg agcaccggtc
     5521 gctcattccc gcgtactggc accagcacat gctctacggc gtgcgcgcgc tgctcgacct
     5581 gcagcccgaa gggctcaagc tcgggttcct gatcagcccg tcggtggacg gcgccgcgcc
     5641 gtcgatgctg gtgcaacgca tcggcgggca cgtgatccgc ggttcctcga cgcacaccgg
     5701 cgcgcgcgcg ctgcgcgact actacgagac gatcgtcaag cagcagatct ccccggccat
     5761 cacgccggac gggccgaagg gcccggtgca cgaattcaag ccgggcgccg tgctgctcgc
     5821 gcagctcacg gggaagccca tcctgctcgt gagcgtcgcc gcgtcgcgaa cctggcgctt
     5881 ccggacctgg gaccggttcg agctgccgct gcccttcagc cgcgtgcaga tcgcgtacgg
     5941 cgaaccggtc cgggtgccgc gcgtgctgaa tgccgacggc atcgcccgac tgcagctcga
     6001 gatggccgac aagctgcagg cactcaacgc ggccgcacac gcagccctac gatccggccg
     6061 gccacgctga gagcggcacc tcctgcacgg acagagacgt gatgggcacg tccgtcgcaa
     6121 tgtgggccac ggttccggcg atcccgaccg gggcgaggcg gaagatcgaa gccccgatgc
     6181 ggccagcggc gtagttaccg tcgagttcca cagccccgac cgcacgcagc ccgcgcgccg
     6241 cggccttgag cacggcctcg gtcgcggtcc agcgtacgag gcgctgctgc gccgcttgcg
     6301 tttcgcgcgt ggcccccggt gcggtgtccg gctcttgcac ttcctccagg tcgaagccga
     6361 cctcgcagtc acggctcaag gcgacgccga cgtgatgctc gccgtgcgag atgctgaagc
     6421 gtggcccgcc cgggacgtac ggctttccgc cggtcggaaa catgagtccg cgaggctcgg
     6481 ccgggctgcc gctcaggagc gcgatgccgt gcagggccag gccgatgccg gcgaggctgg
     6541 cgcggcgcgc atgcgggtcc cgccgctcga gttcgtgccg cttcgcatac ggcagtctcg
     6601 cgagcagggg cgcgtatgcc tcgtcgacgg gtcctggcgg cagccgccca tgcaatacca
     6661 gcacgtgagc catgcgcgga ttctcgactc gtgcaagcga gcccgcaatt ctgggacaat
     6721 tcccctcctc gtgattgcac gctggatccg gcaccgatga tcaagttcga gacgctgccc
     6781 aaggcgctgg atgaccgggc ccggggcacg gtcaccatcg gctacctcga gggcgagaat
     6841 gccgagaaat cgctcaggct ggcggagctg cgccggcgcg cgctgggcat cctgcgccac
     6901 ctccaggaca tgggcgcgcg gcagggcgac acgctcatcc tgcacgtcgc gagcaacgag
     6961 cagttcatcg acgcattctg ggcgtgcctc tacggcggca tcgtcccggt gccggtcgcc
     7021 gtgggcatca gcgacgagca caagcacaag ctgctgcgca tcgcgcgcca gctcgggaca
     7081 ccctggctgt acaccgaccg caagctccgc gagcggctcg cgacgttcgc ggccagcctc
     7141 gaggccgcaa gcctgtggag ggatctcgag cggcgttcgt tcctcgtgga ccagctcgac
     7201 gacgtgtcgc gcgagggagc tcctgcgcga ctgtccagag atgacaccgc gttcatccag
     7261 ttctcctcgg gatcgaccag cgaaccgaag ggcgtggtgc tcacccacgg caacgtcctc
     7321 gccaacgcgg aaggcacgcg ccaggccgcg cgcctcgacg agcaggatgt ctcgctcagc
     7381 tggatgccgc tcacccacga catgggactc atcggctttc acctgatgat ggtgtacgtc
     7441 ggcatccggc agtacctcat gccgacggac ctcttcgtgc gccgcgcact gctgtggccg
     7501 aaattcctgt cggccaagcg cgcgacggtg acctcgtcgc cgaacttcgg ctaccggcac
     7561 ttcctgcgcg cactcggcga caagcagctc gagggcgtcg acctctcggg cgtgcggctg
     7621 atcttcaacg gcgcggagcc gatctcggtc gacctcgccc acgagttcct ggaccgcatg
     7681 cgtgggtacg gcctgcggcc gacgtcgatg ttcaccgtct acggcctcgc cgaggcgtcg
     7741 ctcgcggtgt cgttcccgcc gcccgaggcc ccgatgcggc acatcaacgt ggaccggcgc
     7801 tcgctgggtg tcggtgccac cgcacgcctg gttgcggcga cggacccgac cgcggtcaag
     7861 ctcgcctgcg aaggcaagcc gatcccctat tgcagcgtgc gactgctgga cgacgcgggc
     7921 aacgaggtcg gccccgacca gaccggtcac ctgctgatcc gcggcgacaa cgtcacgcgc
     7981 ggctacttcg agaacccgga ggccaatgcg gcggccgtgt cgcccgacgg ctgggtgcgc
     8041 acgggcgacc tcggcctgtg gcacgacggc gagctgtacg tcaccggccg ctcgaaggaa
     8101 atcatcttcg tcaacggcca gaactactat ccgcacgacc tcgagatgat cctgcagcag
     8161 gagccgggcc tcgagcacgg caaggttgcg gccgcaggcg tgcgcaccgc cggtggcgcg
     8221 accgacgacc tcgtgctgtt cgtcgtgcat cgaggcgaga tggcggactt cgtgcccacg
     8281 gcgacgaaag ccatgcacct ggtgaacgag cacgcgggtg tcgaggtggc gcgggtggtg
     8341 ccgatcgcac ggatgccgaa gaccaccagc ggcaagctgc agcgcgccgc actcgccgag
     8401 tcgtatgcca acggcgagtt tgccgccgag ctcgcggcct tcgacgccgc ctgggccgcc
     8461 gtccacgccc agggccgggc cgcggcgggc gggatcgagc gccagctcaa ggccatcgtc
     8521 gacgacgtgc tcgccggcaa gcacgtggac gtcgacgaca acctgttcga gatcggggcc
     8581 agctccctca cgctgatcga gatccacgag aagatcgacg aactctatcc cggcgtggtc
     8641 gacctgacgg agctgttcga tttcccgacg gtgtcgcagc tcgcgaagca cctcgaggcc
     8701 aagctcggcc agggctgaag gccggcatgc ccgcgtgaca ctggaaacac aggccgcgca
     8761 catcctgcac atcgccgggc agcgcggggt cgtgcgatcc cacgaggtca ccgaagccgg
     8821 gctcgcccgc gccaccctga tgcgactggt gcgcgatggg cagctggtgc agctggcgcg
     8881 aggtgtgtac gccctgcccg gccagcggct gtcacgacgg cacccgctcg cggaagtggc
     8941 ggcgcgcagc tcgcgcggcg tgttctgcct gctgacggcg ctgcacttcc acgggctcat
     9001 cccgcggccg cccgacgcgg tctggctggc catcccgaac aagggccgtg tgccgacggg
     9061 cgggaacgtg ccgctcgaag tcgtgcgctt ctcgggtgcg gcgctggcgg aaggcgtcga
     9121 gcaccacctc gtcgatcgtg tcggcatccg cgtgtatgcg ccgggcaaga ccgtggccga
     9181 ctgtttcaag ttccgcggcc gcgtcggact ggacacggcc gtgacagccc tgcgaaccgt
     9241 cctcaggcgc aggctcgcga cccgcgccga actcgagcac tacgccggcg tgtgccgggt
     9301 ccgcgagacg atgctcccgt accttgcacg tggcgcggtg caggcgcgcg aatgatcagc
     9361 gcagcgcgtc gagcccgaag accttcgcga gcttcgcgtc gaacatcgcc cccggcagcc
     9421 agcgcttgag cagcggatat cgcaggcttc ccgtgccgcg ccggacgacc ctgggcggcg
     9481 gggaccgcaa caatgcatcc acgactggag cagcgaactc atccgccggc atcgcgccct
     9541 gctgccccgc ctgggcccgt gcgtgaatgc cggcttctgc cgcccggtag agcgaatcgg
     9601 ccggcaagcg gacggccgcc tcggcgtgat ccccgaagct cgaacgcact ccgcccggct
     9661 ggatcgccac gacgtggatg ccgaagggct tcagctccat gcgcagcgca tcggtgatgg
     9721 aatgcacggc ggccttgctc gagcaatacg ccgccgcgaa tggcgtcgta aacaggccga
     9781 cgatgctgcc cacgttcgcg accgtggcgc tgccgtgccg cgccaccgcc gagcgcagca
     9841 gaggcatcgc catcgcggtc acccttgccg gtgaaattac gttggtctcg tactggcgac
     9901 gcaggtcgtc gcgcgtgagg tcgatcaccg cgcccacctt ggagaagccg gcattgttga
     9961 ccagcaggtc gagctgtccg gcctcgctca tcaccgtgtc gaatgcggcg gcgatggtcg
    10021 cgtcgtcgtc gacgtccacc tgcaggccac ggatgcccag cggctcgagc ggcgccagcg
    10081 catccttgcg gcgtgccgtg gcgtacacgc ggtgaccgcg cctggcgaat tcgcgggcga
    10141 gtgccgcgcc gatgccgctc gagcagcccg tgatgaagat cgtcttttgc atgtcgctct
    10201 cctggaatcc tggtcgcatc gtgcgacccc gtctcgcctc gcaccgaatc ggcccggccg
    10261 gtcctgccgc cgatcgcgcg ttgcggcctc acgtgccgcc ggaatatacc ttgcgcttga
    10321 gcttccaccg ccggtgcgac caccaccagc ccggcgggtc cgcgcggatc cagtcctcga
    10381 ggcatcgcgc atagcgcgtg gtgacctccc cgcccggcag cttctcgccc ggctcgttga
    10441 gcggcaggta gtcgagcacg taccggccac gctcgagccg gcgcatgcgc acgagcacga
    10501 ccctcgagcg taacgcgcgg ccgagcagct ccggccccat gtagaaggcg gtgtcctgcc
    10561 cgaggaactc gacccagtgc ttctcggggc tcgtacgcgg cacctggtcg gcgatgagcc
    10621 cgatcgcccc gacgtcacgg atggccgcga gctcgcgcag caccgacttc gcgggcacca
    10681 tccgcgcacc gaagcgcccc cggcgatcct tgagccatgc gtcgacccgc ggattccggg
    10741 caggcttgta cagcccgacg aaccgacccg ggaattccag ggacagccgg tacagcatcc
    10801 actcgaagtt gccgtggtgc gccccgacca gcaccagtgg gcgcgacgag ccggtgcccc
    10861 cgagcacttc aggattggca atggtgacgc gcgctcgcaa ttccgcttca cccagggccg
    10921 tggcgtagag cgattcggcg acgagctctc cctggcgcgc ggcgaactcg cgcgccaccc
    10981 gccggacgcg atcgcccggc cagtcgggaa acgatcgctg caggttgtcg cggatgacgg
    11041 agcgccgcag gccgaatacg cggtacccga gccacgccgc gacggctccg atcgcgtagc
    11101 agcagcgcag acgccagccg tcagtcatac atgcgcctcg cgcgcttcca gcggttgtat
    11161 gcccagaagt actgcgcggg ttgcgaccga acggcgtcct cgacggccct cacgtacgct
    11221 tcgagcgcgc gttccgggtg tgcccgctcg ccggcctgga gcagcggaac gaatcgtgcg
    11281 acgtagcggc cgcgtccttc gcggcgcatg cgaatgtaga ccggctggaa ccccagtcgc
    11341 gcgctgatcc agccgggacc cgcgaagaag tccgtttccg tgccgaagaa ccgcacccag
    11401 tgctgctgct cgcggcccgc ggccggcgac tggtcggcca ccagcgccag gagcctgacg
    11461 gcgccgcgct gcgccagcag gtgattgacc acctgcttcg ccggcaccat ggtcgcgccg
    11521 aaacgactgc gcatcgcgcc gagttcgcga tccgcgcgct cccggctcgc cggcttgtac
    11581 gcggccgtga gcggcacgcc gaacgcggtc gagcagcgca gcagcaacca ctcccagttg
    11641 cagtgatggg aggccagcag cagcaccttc ttgccgtcgc gcaggagatc ccgcaccacg
    11701 tcgtcgtctt cgaacctgag ccgatcgacg agatccgacg cctcgatgcg cgtgccgtgc
    11761 acggcttccg caaccagctc gccgaggtac cggtagaagt cccgtgccac gcgtcgccgc
    11821 tccggctgcg gggcatccgg caggcagcgc gcgatgccgt cgcgcacgaa gcgcctgcgc
    11881 cagccgacga cgcgcgtcac gaacgcgagc gcgccgcaca ccgcgtagag gacgggcagc
    11941 ggcaggagcg cgaggaaccg caacacggat caggtggtgc cggaggaggg actcgaaccc
    12001 ccgacccgcg cattacgaat gcgctgctct accaactgag ctactccggc gggaggggcg
    12061 cgaagtatac ggattcggcc cttgggcgtg aagctttcca ccccggggac cgccgtcacg
    12121 cggtgcggcg caaccggatg acgacatcca cgccggccac ctccgttccc gggggcagcg
    12181 ccggcatctt cgcgaaactg atgtcctgcg gcgggatgtc gagcatctcg ccggtggcca
    12241 ggtcctggaa gtgatagtgc ggctccgtca gcgagtcgta gacgcatcgc tccggatccg
    12301 tgtgaatcgg gtgcagcagc cccgcttcca cgaacagctt cagcgtgttg tacacggtgg
    12361 ccttcgagac ccgccccgcg gactgccgca aggtctcgag gatctgctcc gcggtgaggt
    12421 gccgcggctc cgtcagcagg agttcggcga ttcgcaagcg ctgcgccgtc ggccgcaccc
    12481 cgtgccgcgc caggatcgac tttgcccgag ccgtggcgcc agccgccgat gtggattcgt
    12541 gggacatcgc gttgaattat acgtgccgct gcgctcacgc gcattttgcg gcaaatacgc
    12601 cctaaattgg ggctgaacgg ccgcccggcc aggcctagat tccaggcctc gagtgggcga
    12661 gcgggagcag gccatggaac ggccttcggg attcaccctg ccggaactca tgttctcgat
    12721 ggccatcgtc gcgggcctgg tcggatggag cgtgccggcc ttccgtgaca tccggctgaa
    12781 tgcggcgcgc accagcgagg tcaacctgtt cctccaggcc gtctacctcg cccgcagcga
    12841 ggcaatcaag cgcaacggcg tcgtcagcct ctgcccgacc ctcgacggca cggcctgcgc
    12901 gccgcccggc tcgacctggg accgtggctg gatcgtcttc gcgaaccgcg accgcgactc
    12961 gccggcagtc cgcgacgcgg gcgaggaact gctgtcggcg tatccggcct gggaaggcgg
    13021 cagcatcgtc gcaaaccgcg gcaccctgtc cttccgcgcc ttcggccaga gcggagtgac
    13081 cgccacggtc acgttctgcg attcgcgggg cagcggacac gcacgcgccg tgatcatcag
    13141 ccaggcgggg cggccgcgcg tggccgaccg gaattcgtcc ggggccgcgc tcagctgcac
    13201 gtgacatgga cgcggcattt cgcaagcacg gcggcgcaca gggcggattc tcgctggtcg
    13261 agatcctggt cgtggccgca atcgtcgccg tcgggctgct gggaggggaa gcactggtgg
    13321 tgacgtcgct cgaagccagc cgcagcgcca tgctgcagac catcgcagtc gatctcgccg
    13381 ctgatctcgg cgaccgcatc cgcgcgaatc cgtcggccgg atcggcgtat ggcctgtcgg
    13441 caggcgcgcg tctcggtccg ccgccgaagg cctgccggac cgtcggggaa tgcaactccg
    13501 ccgacctcgc cgcggtcgac ctgtacctgt ggcagcaggc cacgttcacg gcgctgcccg
    13561 aggccaccac cgtcgtgcag gtggagcccg ccggcggcgc cacgcatcgc tacacgatcg
    13621 tcctccgctg gacggccacc gggcggccgg ccgaatcccg tgtcgcagcc acggtcgaga
    13681 tatgagcgtc gcgaagaccc tggcggcacg ccagcacacg tcctgcgtcg gcgcaggtct
    13741 cgtcgaactg atggtggcgc tggtcatcgg tgcattcctg ctgtcggcga gtgcaacggc
    13801 gtacctcggc gcacgcaacg cgcgtgcgca actcgacgca ggcgcgcgcc tgcaggaaat
    13861 cgcacgctac gccatggcgc tgatcgaggc ggacgtgcgc atggccggct actgggggct
    13921 cacgaaccgt gccgacgaca tcgccgcgca tccgtccctc gcgttcccgg cgaaatgcgg
    13981 cggcaaaccc tgggtgacga gcgccaccca gttcgtcgcc ggaacgaaca atgcgtacct
    14041 cgccgttccc tcgtgcgcgg ccctgtccgg cggcgcgcag ccgggcagcg acgtcctggt
    14101 cgtgcggcgc gccagcgcgc agcgcatcgc gccgcagaag gctacggtgg gctcaaccga
    14161 ccaggaccgg gtgctcgtcg tgtcgagcca cgcctcgggc acggtgttcg tcccccagga
    14221 cctcggcaac cagattccgg ccagctacgc cacgaccgac gtggctgggc ttgcgccgca
    14281 ggcagacacc cgcgcgctgg tcgtcgacgc ctactacgtc agccgcggat cgagtacggc
    14341 cgccgacttc cctgccctgc gccgcaagac cctgaccgcc gggcccgaca tcggagacga
    14401 agaagtcatc gccggcgtcg aagacctgca gttccaggtc ggagtggaca ccgacgccga
    14461 cccgggtgca gacgtgtatc tcgatcctgg catgctgcct accggtgcac ggatcgtctc
    14521 agtgcgcatc tggctgcgcc tgtgcgcgcc cgatcgcggg acggcactgg ccacgcagcg
    14581 catcggtccg tatggcgatc gtgccgcagg gatcgtcacc gacacgcggc tgcgcctgct
    14641 ggtgtcgcga acggtctacc tgaggaactc cgggtcatga atcggctgcg ttgcgtgaat
    14701 cgacaacacg gcgtggtgct ggtcgtcgcg atgctgctcc tgctcgtgct caccctcctc
    14761 gggaccacgg gcctggtcac cgccgtgctg gaactgcgga tggcggcgaa cgcgcgttac
    14821 caggcgcgag cattccaggc ggccgaatac gggatccagc aggcggtgca cgcgacgggg
    14881 ctcgcgacca cgatcacgtt ggcgagcccg aggctcgagc cagcggctgg cgcgcccctg
    14941 cgagtccctg gaactccgca ggactcgtat tcctatcgcc tctattacga cacgagcact
    15001 ccgcccccac cgctcgtcga cggcacggtt cccggtccgg gattgcgcgc ctatcacttc
    15061 gtggccgagg caaccggtcg gtcggagcgc ggtgcgacgg aaacgcatgt ccagggtttc
    15121 tatctgatcg ccagcgaccc accacccgcc tcgttgacgc caccggcctg cgcgccaggt
    15181 tgcagtgagg cggcgctgta cgagccgcac cgcaccttct ggatgcaagt gaatgccgag
    15241 tgacatgcgc acacgaacga gtctgtggcg agcgcgccgg cacgcgccgg cacgcgccag
    15301 cggcggagga ttttcgctgc tcgacgtcgt ggcagccatg gccatcgtgg ccctgctcgc
    15361 gtgcatcgcc ctgccgagct accgcgcgca gatgatgcgc gccaaccggg ctgcggcccg
    15421 cgcggcgctg atggcgctgg ccgcggagca ggaaaagttc tacgtcgagt gtcatgccta
    15481 cgcgaccagc ctcgacgggt ccgccgccag cacctgcggc acgtcgacgc tcggcttccc
    15541 ggcgagcgcc ggcgacggaa cataccgcct caggatcacg gaaggcgatg cttccgggtg
    15601 gactgcggtc gccgaggtcg tgccgggcag ccggcaggag gatgaccggc gctgcagggc
    15661 gctgcagatc gacgaggtcg gtcacaggac ggcgacgacc gcgtccggtg acgccagcga
    15721 cgcagagtgc tggagccggt agcgaagcgg ccgccgctcg aggggctagg aatccgtgcc
    15781 gcgcgccgcg cgcagttcgc gtggcagtgc gaacacgacg ttctcctcgc ggccggtcac
    15841 ttcgtccgcg gcctgcccgc cccactcgcg gagacgtgcc accacccgtt gcacgaggac
    15901 ctccggcgcc gacgcccccg ccgtcacgcc gacggtggcg cgcccctcga accactcgcg
    15961 ctcgagatcc tcggggccat cgaccaggta accggggatg cccgccttct ccgcgatctc
    16021 gcgcaggcga ttcgagttcg aactggtccg cgaccccacg acgatcacga cgtcgcagct
    16081 ggcgacgagc gacttcacgg catcctgccg gttctgcgtc gcgtagcaga tgtcctcctt
    16141 gcgcggcgtg gtcagcgtcg ggaagcgctg cttcagcgca tcgaggacct ccgccgtgtc
    16201 gtcgaccgac agcgtcgtct gcgtcacgac cgccactcgt gccgggtcgc ccacctcgag
    16261 ccgctcgacg tccgtcgcat cctccacgag gctgatgcgt ccgcccatcg tcgagtcgaa
    16321 ctggcccatc gtgccctcga cttccgggtg gcccgcgtga ccgatcagga tgacttcccg
    16381 cccgtcgcgg gcgaagcgcg cgacctccat gtgcaccttg gtcaccagcg ggcaagtcgc
    16441 atcgaacacg cgcagccccc ggcggcgcgc ttcgtcctgc accgccttgg agaccccgtg
    16501 agcgctgaaa atcacggtcg cgccgtcggg cacctcgtcg agctcgtcga cgaagatcgc
    16561 ccccaggccg cgcagccggt cgaccacgaa acgattgtgg accacctcgt ggcgcacgtg
    16621 gatcggcgcc ccgaacagct cgatggcgcg ctcgacgatg tcgatcgccc ggtccacgcc
    16681 ggcgcagaag ccgcgcggat tggcgagcag gatcctcacg cccgccgtcc gcggaatgca
    16741 tccacgatca gcagcatcgc gccgatcgtg atggcgctgt cggcgatgtt gaaggcgggg
    16801 aagtacgcat cgccccagtg ggcgtggatg aagtcgacga cgtagcccag gcgcacgcga
    16861 tcgatgacgt tgccgagcgc gcccccgagg atcagcgaca gcgcgaacgg cagcagcttg
    16921 tcgccacgcg gcgttcgcca gatccatgcg accagcagca cgctcaccac gatgccgagc
    16981 gcaatgaaga acggccgctg ccagccggaa gccccggcca gcatgctgaa ggcggcgccc
    17041 gtattgtgca gcagcgtgaa gtcgagcacc ggcaggacct cgacgcgctc gtacagctcg
    17101 aagaatcgca gcaccaggaa cttggtcgcc tggtcgagcc cgatgaccgc gagcgacagc
    17161 cacagccatg cggccggatg cgtgcgctgc acgagtggcg gctggccgct ctccgacgcg
    17221 gatgtatcgt ccgtcacgtt cacttacgcg aacctccgga tctcgcccgg cccgtcgacg
    17281 ttcgacgcgc agcgcgcgca gagctccggg tgggcggcca cgctgccgac gtcgttgcgc
    17341 ttgtgccagc agcgcacgca cttcgtcgcc gtcgacgcat gcacgtgcag ccaaagcgtc
    17401 ggcgcatccg cgggccccgc gggcaccgcg ctggtcgggc gcgaatcggc cgggtgcaca
    17461 tgcgcctccg acgtgatgaa cacgaagcgc agctcgtccc ctattgcgtc gagagccgtg
    17521 ctcgtcgccg cgtccgcgta gacctcgacg atcgcgtcga gcggcgcgcc gatctcccct
    17581 gcggcacgca gccgctcgag ttccttgagc acggacgacc gcacgtcgag caccgtcggc
    17641 cagtcgatcg cgcgggcctc ggcagcgggc gccggcaggt ccgcccaggt cgcgtggaac
    17701 accgattccg cgcgcgcgcc cggcatgtgc cgccagatct cctccgccgt gaacgacagg
    17761 atcggcgaca gccaccgcac catcgcctcg gcgatccagt acatcgcggt ctgcgcggag
    17821 cgccgcggca ggcccttcgc gggggtcgtg tacaggcggt ccttgatgac gtcgaggtag
    17881 aagccgccga ggtccacgac gcagaagttg tgcaccttct ggtagatcag gtggaagtcg
    17941 tagctgcgat acgcgtcgac gacctcctgc tgcagttcgc gcgtgcgcgc gagcgcccag
    18001 cggtcgagtg ccaccagctc gctggtgggc acggcatcag cagctggatc gaagcccgcc
    18061 atgttgccga gcaggaaccg gaccgtgttg cggatgcgcc ggtaggactc cgacatccgc
    18121 ttcaggatct cgtccgagac ggcgatctcg ttggcgtagt ccgtggaagc cacccacagt
    18181 cgcaacacgt cggcgccgag cgtgctcacg accttctgcg ggaggatcgt gttgccgagc
    18241 gacttcgaca tcttgcggcc tttctcgtcc acggtgaatc cgtgggtgag cacgcccatg
    18301 tacggcgcgt ggcccttctg cgcgaccgac gtgagcagcg acgagtggaa ccacccgcga
    18361 tgctggtcgg aaccctcgag gtagaggtcg gccggcgtgc gcacctccgg gaacaggcgc
    18421 gggacgcagt ggtgcgacac gccggagtcc acccacacgt ccatgatgtc ggtgaccttc
    18481 tcgtaatgct ccgcctgggc acccagcaac gccgccgggt cgaggtcgaa ccagacgtcg
    18541 ataccgtcct tctccacgag atccgccacg cgctcgacga gcgcgggggt gtccggatgc
    18601 agctccccgc tctccctgtg cgtgaacagc gcaagcggca cgccccaggt gcgctggcgc
    18661 gagatgcacc agtccgggcg gcccgcgatc atgccgtaga tgcgctgctc gccccagtcc
    18721 ggcgtccatt cgaccttgcc gatctcctcg agcgccttcg cacgcagtcc cgcctgctcc
    18781 atgctgatga accactgcgg cgtcg
//
LOCUS       DAOMFD010000023        18288 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_589_length_18288_cov_34.473866, whole genome shotgun sequence.
ACCESSION   DAOMFD010000023 DAOMFD010000000
VERSION     DAOMFD010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 18288)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18288)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18288
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_589_length_18288_cov_34.473866"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..509
                     /locus_tag="PL152_02665"
     CDS             <1..509
                     /locus_tag="PL152_02665"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR48208.1"
                     /translation="YMYGRHCFQSGAFDRAVPLFEAAVRLRPDDYQALCILEGALLKI
                     GRMEAHGEVAQRAMAAIERQLAIDPQDGRALQLGTVQAARLGKRERTHELARRVLAVR
                     PDAFSTFYNVACAYAVLGDVDEALDLLDRAVRHGRGNLEWIEQDPDFDSLRSDPRFNE
                     IVNRLRST"
     gene            524..1759
                     /locus_tag="PL152_02670"
     CDS             524..1759
                     /locus_tag="PL152_02670"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR48209.1"
                     /translation="MGEEVSRRSAILRNADFTRFIAARFLASLAVQMQAVAVGWQVYA
                     VTRDPLDLGLVGLSQFLPFVVLILPAGHLADNHDRRRIVSACFALQCLCALLLLGFAW
                     RGLASAWPVFAVMVLFGIARAFSMPTGQALLPNLVPREQFATAVAINSSTWQVSTIAG
                     PALGGIVYLAGATVVYATVAALLAAAVVLMLMVNGGGERAAAAEPPGWHALLSGLRFV
                     WSRRPVLGAISLDLFAVLFGGATALLPVYAADVLHVGPSGLGLLRTAPGAGAALCGIV
                     LGIAPISRLVGRWMFGGVVAFGVATVVFGVSRDFWLSVTALAVMGAGDMVSVYIRHLL
                     VQLETPDAIRGRVSAVNAVFIGASNELGEFESGITAAWFGTVPAVIVGGCATLVVAGA
                     FARLFPELRDMDRLPDAAP"
     gene            1791..>2298
                     /locus_tag="PL152_02675"
     CDS             1791..>2298
                     /locus_tag="PL152_02675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQR48210.1"
                     /translation="MDREERTRVEKGRGAVSNPAGRFQATRAVAADDGWGSLDVPPRR
                     AETVLVPDRPRHAITHNDSPDVPFDQSVNPYQGCEHGCIYCFARPSHAYWNLGAGLDF
                     ETRIFHKPGLARLLERELSAPGYVCKPINLGANTDPYQPAEREFRTTREVLEVLLAHR
                     HPVTIVTKG"
     assembly_gap    2299..2398
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2399..3031
                     /locus_tag="PL152_02680"
     CDS             <2399..3031
                     /locus_tag="PL152_02680"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQR48211.1"
                     /translation="VLEVLLAHRHPVTIVTKGALILRDLDLLSELARQRLVSVHVSVT
                     TLDDDLKRVLEPRAASPAARLRVIRELADADVPVGMLLAPMIPALNDHELERLLEAGR
                     DAGASRAGFILLRLPRELATLFEEWLREHFAARADRVLNLLRDLRGGRLNDPRFGHRM
                     RGEGPYADLLSARFDVACRRLGLVTGRRHDLDTAAFRSDPGAPQQGVLFG"
     gene            3238..3933
                     /locus_tag="PL152_02685"
     CDS             3238..3933
                     /locus_tag="PL152_02685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48212.1"
                     /translation="MTSKVDETRHVAAAIQPNESIVILKKPQLEGVGTEGEFLSCVQE
                     KLGGELVHPQDGQSAKPASSSPVPFKIYGEQQFEDAMFPWFEPSTAPANAAGMKTLLE
                     HPGITERLTQIGVRYVVWLDGNTRKTDGGGSVACAIGPGGGGCFGVGWWDKEADYVAT
                     VWDLNTATELGTVTADVTGTSVLIGAVAPIPIITPVQGKACSRLSEQLRSFLTGSDLV
                     APAPAAATGSGGY"
     gene            4007..>4311
                     /locus_tag="PL152_02690"
     CDS             4007..>4311
                     /locus_tag="PL152_02690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48213.1"
                     /translation="MPKTMHRLLWSAVCAAGVAGCASSTSTQSSSMPQMPSPSSSPSS
                     QSSPSSGGSPSTGQGSGAGQPSSGGQPSAGGGQGSGSGGQESPFPSQRSQGGSPSSGG
                     "
     assembly_gap    4312..4411
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4412..4767
                     /locus_tag="PL152_02695"
     CDS             <4412..4767
                     /locus_tag="PL152_02695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48214.1"
                     /translation="GSEAAGENGDGSAGDGTAGGDGKSGAGTSGSAAGTGGDDGGRGE
                     SAGGTSGTQGGGGGYGGGPKGGAGPNTVPPDIPDGSDDDIVARQLREAAMKETDPELR
                     EKLWDEYRKYKQSTQ"
     gene            4764..6014
                     /locus_tag="PL152_02700"
     CDS             4764..6014
                     /locus_tag="PL152_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48215.1"
                     /translation="MSRLATSPGLRAASFAAMAVLLLAGCVVQDERPMPRVIAQKATT
                     EVPQDELLDVGVRLFDPNVPKDPKKLEDGEIYPEVRDAESRYIPVEIRDTLEGTGQWG
                     QVRVLPRDATGMDVYVDGKIVESDGRELKLDVTVSDSSGRLWYHKEYEGAADTRAYKD
                     VASKPADPFENVYATLANDLLAARAKLTREQRVQLHEITDLRFDADLAPYAFGQYLAK
                     DRKGYYAITRLPAADDPVLQRMDRVRERDYALMDTLNEYYTNFAESMAVPYTSWRKAT
                     YEELEARSKAQREALTRQILGAVAVVGGIVAGSNSNSSAASAAATAAVIGGMYAFKSG
                     LDQRAEIRTHTESLKQLGDSFQSEVQPMVVDVEGRTLQLKGSAEQQYAEWRQLLKQLY
                     ENETGLPATGGTAANGTPTAATKP"
     gene            6034..8187
                     /locus_tag="PL152_02705"
     CDS             6034..8187
                     /locus_tag="PL152_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQR48216.1"
                     /translation="MTFESGQVLAARYALERRLGDGGTAEVWLARDGDARCVVKVLRA
                     ERAREPAERARFVAGARLQRATQHPNVLACIDVVESEPPFAVFSFESATDLAGMRGAP
                     SRQLVPVLSQVAAGLGALHANGHVHRDIKSSNVLMTEDGRVLLADFGLAAAIGDATAA
                     AGGSPFTSSPEQLAGSAPSIADDVYGFGALAFELATGYPPFYPDDAAARTAAEPPRLG
                     ERRSGVPAALEDLVTRCLARNAADRPRDLTEISAALARMAAAADAPDAGRTRRPTVAL
                     RVPDAGPAAIEPSWQRAASTGPSAAELRSQGFRRGLLAGSFVFLLVVAGLTFFALPDW
                     VQRNEPVVAAPPPAMPAKPAQAAATPPAKDPQAEAHRALQLKQEHEQQVAAQVRAALA
                     AGAAAIDAGDSAEAQRQYGIAASLDPTNAAARRGLNRAGTLDEVRTLLAQAAEAERTG
                     RLADAERAWGRALELDPDTTAAREALARVRSEQAAGAFSAAVADGLAALSRKDYAAAR
                     AAYERAGRIRPGAPEVKDGLEQVDRALGDRTIAQHLDAARRAEGEERWRDALAEYRAA
                     LVIDPNLLDAQQGVERVEPRVMLDAALASYLERPERLFSSDVRGAARVTLAQAGAIAS
                     PGPVLTRQMAQVTNLLAAAETPVRLTIASDNVTEVTIYRVGRLGAFEHKDMDLLPGRY
                     TVVGTRAGFRDVRREVTIMPGKQPPQVVIRCEEQI"
     gene            8190..10652
                     /locus_tag="PL152_02710"
     CDS             8190..10652
                     /locus_tag="PL152_02710"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HQR48217.1"
                     /translation="MVLTLREPLGERTFAAADFPLSVGGTGSDIVVPAPAPGPLAWFA
                     LHDGQLFVQPAADDAHILHNGSSVTASTWLRGGDVLDVGGGRLKLRSDPGGPVLDLLS
                     GGDGNVTAPPVVPASESVAGEGADEAERIEAIAFRLQGPRAQRAGWSPRRIAVVAVAA
                     LLAIIAALVFTSTPVEVQIEPAADSVVFEGTWPGLRLGTSHLLRPGQYTLVARRAGYA
                     ELRAPVTVRRESGQVLRYRLVALPGRLRIDLPVPGEVTVDGRAMGKAPGEFELPGGMH
                     ELRIHTERYLDYATKVRIAGYGKRQAIAPSLTPAWAVVRMSTEPAGASVRVGGEARGV
                     TPLELELMAGSHRVELLHAGYKPWVSDVQVKANEPLTIGPVRLGLPDGRLAVRSRPSG
                     ASVAISGAYRGRTPLEVDVRPDLAQTVSVTREGYEPASQQVTVGPGKAAVVEVALTPI
                     LGDVIVRASPADAQLYVDGELRGAPNQTLQLTATRHSLEIRKPGYAPYRTTVTPRPGL
                     PQSIEATLLEGVMPATASAQPSAGAAAGATPATTDAVALQPILHTKAGQDLRLVPAGT
                     YTMGSPRREAGRRANESQRPVELKRRFYVATREVTNAEYRQFRPEHRSGYVMQTTLDL
                     DRQPVVNVSWQEAAAYCNWLSGQDGLTPAYEMKNGAFVTVVPATNGYRLPTEAEWEWI
                     ARRDGGGLRKYPWGDALPVPTGAGNYADRSAQALLPVVLTDYDDGYAATAPVGSFAAN
                     PSGYFDLGGNVAEWTHDLYTVQPRSETAAVDPAAAGEGAMHVIRGSSWKSSSVTELRL
                     AYRDYGDGRRNDLGFRIARYAQ"
     gene            10615..10944
                     /locus_tag="PL152_02715"
     CDS             10615..10944
                     /locus_tag="PL152_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48218.1"
                     /translation="MTSDSASRVTRSKRSLVAAVALFAGGLVAVGALRAQEPQSPAQT
                     QPAAPPAEDKAQPPASAPPAGRAQPTDGKTPPASRGAAATGQTQEHFEPTEKIRADFE
                     VSFPVDI"
     gene            10965..11771
                     /locus_tag="PL152_02720"
     CDS             10965..11771
                     /locus_tag="PL152_02720"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR48219.1"
                     /translation="MPRINARAPSELWFSVFSLIVFTIVVHATYVTVVRPRASAVLAE
                     QRAAMKKDPNYVAERSIYVVLKDWEQESEVILGLWAFAIIGYKAWHSRRERALLARDL
                     VHVPDGMKILPEDTREYARPLEALPPTESECLLPRALLVALYRFAATRNVQDVSDAAR
                     SVCSAEADRLDSELSMLRYIAWAIPAIGFIGTVRGIGDALGEAHKAVTGDVSGVTEGL
                     GTAFNSTLAALLISIVLMFLLHQLQLAQERLVLDSETYLDRNLIRHMQLR"
     gene            11776..13152
                     /locus_tag="PL152_02725"
     CDS             11776..13152
                     /locus_tag="PL152_02725"
                     /inference="COORDINATES: protein motif:HMM:NF012708.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQR48220.1"
                     /translation="MTLALEINDAGLVLARDGGILADEPGIVMLDRPTPETGAAAALR
                     ARLQPLYAETRHWQDLGTEPLARPVPAAADRAEVAYAQLAAFVAPHLAGGADTLVAVP
                     PWYTREQLGLLLGIARECGLEVVGLVDAGLAAASLAPAAGTMLQLELYLHRAVVSVLD
                     HTGGLRRTRFDLLPQHGWLGLQQAWLDAIASAFVLRTRFDPLHQAATEQRLCDGLRGW
                     LDASLAGGDVAIEIDSGAGPQAIDFPAGDFVAAAARTYDEYVRVLQRARPVGGPMHLR
                     LSDRFAKLPGLAARLAEIRDSELQWLPRGAAALGALAWEKHIRQGNRSVTLVQHLPVE
                     LQTSAGITPLVVAASVPADARPTHVVHAGRAHPLGPRPLVLGADVPPGRRALRVAPGP
                     GISRAHCSIVLEGGAAWLEDHSTYGTFLNGERVGGRVELRAGDQLRIGSPGVTCELVR
                     AVEDDGAT"
     gene            13139..14230
                     /locus_tag="PL152_02730"
     CDS             13139..14230
                     /locus_tag="PL152_02730"
                     /inference="COORDINATES: protein motif:HMM:NF024909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HQR48221.1"
                     /translation="MARRKTEVFSLSFLDVIACGFGAVVLFYTILSAQAGVQRQHRND
                     ELQSEVNRLEEQVLDGYKNLVVLRNALQSDEKQVPAEGLANRIIEETEQLRQQLAEAE
                     QETLSRREAIERLKQDLKSLDEGKRRLEAGTPSPGQPGTRIKGFIGSGDRQYLTGLKV
                     GGQRILILVDVSASMMDESVVNVIRLRNMADVRRMAAPKWRRTVATVDWLTSQLPIKS
                     QFQVYAFNTQARPLVPGSAGKWLAASDAAALNDALKELRKVVPQDGTSLENALAAAGS
                     LSPKPDNILLITDGLPTQGASPPLVRKTIDGDGRLKLFEHALGRLPSGIPVNVILLPM
                     EGDPMAPSAYWTLARRTNGSFLSPAKDWP"
     gene            14227..15240
                     /locus_tag="PL152_02735"
     CDS             14227..15240
                     /locus_tag="PL152_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48222.1"
                     /translation="MKNSRLGRRDVEVFSLSFLDAICCGFGAVILLLVLTEVGEPVVI
                     EKSREDLQAQVLKLEQELNDIRGTSEVLNRELKARRSQLSEEQLRIARLRGDLSALRG
                     QYESSRKEAEVQNQIEGQLVSAQQDLTEEMKRLLGAQYRRKEDSPIGGLPVDSEYVIF
                     IIDTSGSMANYEWALMQRKLQEVLDAYPTVKGWQVMNDEGTYLFPTYRGRWLPDTPMQ
                     RKLVLERMRDWFPFSNSSPVEGIVEAIRTYYATGLKISLYYFGDEFTGSSIDSVVRTV
                     DMLNREDKTGQRRVRIHALGFPVRPDAPQVTSIRYATLMRILCERNGGTFVGLNEPGS
                     YAR"
     gene            15257..15982
                     /locus_tag="PL152_02740"
     CDS             15257..15982
                     /locus_tag="PL152_02740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48223.1"
                     /translation="MRRMATAAQARVPRHGPDGRKASPRAPRSWLRRASWAALALLAG
                     CGTTEFEAKPTIPEPLVTKIPLVVGVYVPAEFRDKVYKEKRDGGDYSIAIGKAQSEAF
                     MRLMGALFERVIPVDAADAGARTDPQIRGVVEPVLEDVAFVTPVESGTDAYAVSLRYR
                     INGYRPDGQVIDSWIFTGYGAAASESMLGGGAEVLKKALHLAMRDAAAKLATEFREQA
                     MIRGLLPAESTPQAPVEVAPPPP"
     assembly_gap    16062..16161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<16162..17530)
                     /locus_tag="PL152_02745"
     CDS             complement(<16162..17530)
                     /locus_tag="PL152_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR48224.1"
                     /translation="MSSATLLAICSQACLAQTQPAPASTVPAADVRQLLDAKQYDAAV
                     AQARQQLAAAEQQPGPGGDYLQVALMNLALAQYFDEDYLGAEDSYLRVIKLIEDSGRR
                     TTARLARAQAGLATTYYAGKRYDLAAARYEQAIALVRREQGLFTEEQLPYLEKYASAL
                     TELDRVSDALKVRRYILRTVERKYGASSVQYAQALESAGRWLMHVGSYDAARTALRQS
                     IGIIEGQKGENAGELIGPLTALGECARLQLLDPSSMAPSPDQPPRPMFGESLPMVTTA
                     MSATSVTSDGQRSLERAVAIATQLDPPSPAQVADTRTLLGDWYQSRQLPDKAAANYEL
                     AWRAAGTATVAGRQLTDLLFGKPVLIAYVPPTAATRFASRSPDEVVVRNVEVRFTVTS
                     RGRVADAKVLEDAGDPSHGTSVQRALQTAIYRPRFENGVAVDTPGVTLDQRFYVLVDE
                     DKSTDK"
     gene            complement(17620..18231)
                     /gene="amrA"
                     /locus_tag="PL152_02750"
     CDS             complement(17620..18231)
                     /gene="amrA"
                     /locus_tag="PL152_02750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HQR48225.1"
                     /translation="MAATPSTPLDPVARGLLLRIAAQSIETGLTRAPDEPPATAALPA
                     ELQLQRASFVTLTVEHRLRGCCGTLEPRRALAFDVWQNARASAFGDPRFAPLARSEWE
                     EASLEISVLSPLERVEAGSEQSLQRRLRPCVDGLVLTWRGQRATFLPKVWEQLPDPGE
                     FIRHLKAKAGWDADFWAADVESWRYTVETMAVQRPARGMRWTG"
BASE COUNT         2706 a         6208 c         6444 g         2630 t
ORIGIN      
        1 actacatgta cgggaggcac tgtttccaga gtggcgcgtt cgaccgcgcc gtgccgctgt
       61 tcgaggcggc ggtccggctg cggccggacg actatcaggc actgtgcata ctggaagggg
      121 cactgctcaa gatcggccgg atggaggcgc acggtgaggt ggcgcagcgc gcaatggcgg
      181 cgatcgaacg gcagctcgcc atcgatccac aggacggacg cgccttgcag ctcggcacgg
      241 tccaggccgc acgactcggc aagagggaac gcactcacga gctggcccga cgcgtgctcg
      301 cggtgcgtcc cgatgcgttt tcgacgttct acaacgtagc gtgtgcctac gcggtcctcg
      361 gcgacgtcga cgaggcgctc gacctgctcg atcgcgcggt gcggcacggg cgcggcaacc
      421 tcgagtggat cgagcaggac cctgatttcg attcgctgcg gtcggacccg cggttcaacg
      481 agatcgtgaa ccggctgcgc agcacctgac ggcgcgtcac gggatgggcg aagaggtctc
      541 ccggcgttcc gcgatcctgc gcaacgcgga cttcacccgg ttcatcgcgg cacggtttct
      601 tgcatccctg gccgtgcaga tgcaggccgt ggcggtcggc tggcaggtgt acgcggtgac
      661 gcgcgacccg ctcgacctcg gcctcgtcgg gctgtcgcag ttcctgccgt tcgtcgtgct
      721 gatcctgccg gccggtcatc tcgcggacaa ccacgatcgc cggcgcatcg tcagcgcctg
      781 cttcgcgctg cagtgcctgt gcgccctgct gctgctcggc ttcgcgtggc gcggcctcgc
      841 ttccgcgtgg ccggtgttcg cggtgatggt gctgttcggg atcgcgcgcg cgttctccat
      901 gcccaccggg caggcactgc tgccgaacct cgtgccgcgg gagcagttcg ccacggcggt
      961 cgcgatcaac tcctccacct ggcaggtgtc gaccatcgcg ggcccggcgc tgggcggtat
     1021 cgtctacctc gcgggcgcca cggtcgtgta tgcgacggtc gcggcactgc tggccgcagc
     1081 ggtcgtactg atgttgatgg tgaacggcgg gggagagcgc gctgcggcag ctgagccgcc
     1141 gggctggcat gccctgttgt cgggactgcg attcgtctgg tcgcggcggc cggtgctcgg
     1201 cgcgatctcg ctggatctct tcgcggtgct gttcggggga gccactgcgc tgctgccggt
     1261 ctatgccgcc gacgtgctgc acgtcggtcc cagcggcctc ggcctgctgc gcacggcgcc
     1321 cggggccggc gccgcgctgt gcggcattgt tcttggcatt gcgccaatct cgcggctggt
     1381 cggccgctgg atgttcggtg gcgtggtggc gttcggcgtc gcgaccgtcg tgttcggggt
     1441 gtcgcgcgac ttctggctgt ccgtcaccgc actggcggtg atgggcgccg gggacatggt
     1501 gagcgtgtac atccgccacc tgctcgtgca gctcgagacg ccggacgcca tccgcggacg
     1561 ggtgagcgcg gtcaatgcgg tgttcatcgg ggcatcgaac gagctgggcg aattcgagtc
     1621 cgggatcacc gcggcctggt tcggcacggt gcccgccgtg atcgtcggcg gttgcgcgac
     1681 gctcgtcgtg gccggcgcat tcgctcgtct tttcccggaa ctgcgcgaca tggaccgttt
     1741 gcccgacgcc gcaccctgac agctgtataa acatacagta tgctcgtccc atggatcggg
     1801 aggagcggac tcgggtcgag aagggtcggg gcgccgtcag caatccggcg ggccggttcc
     1861 aggcgacgcg agccgtggcc gccgacgatg gctggggttc cctggacgtg ccgccgcgac
     1921 gcgcggagac ggtcctggtc cccgaccggc cgcggcacgc catcacgcac aacgattcgc
     1981 cggacgtccc gttcgaccag tccgtgaatc cctaccaggg ttgcgagcac ggctgcatct
     2041 attgtttcgc gcggccttca catgcgtatt ggaacctggg tgcagggctc gatttcgaga
     2101 cgcgcatctt ccacaagcca ggcctggcgc ggttgctcga gagggagctg tccgcgccgg
     2161 gttacgtatg caagcccatc aacctcgggg cgaacacgga tccttaccag cctgcggagc
     2221 gcgagttccg gacgacgcgc gaggtcctgg aagtgctgct cgcgcaccgt cacccggtca
     2281 cgatcgtgac caagggcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     2401 cctggaagtg ctgctcgcgc accgtcaccc ggtcacgatc gtgaccaagg gcgcgctcat
     2461 cctgcgcgat ctcgacctgc tgtcggaact cgcaaggcag cgcctggttt ccgtgcacgt
     2521 cagcgtcacg acgctcgacg acgatctcaa gcgggtgctc gagccgaggg cggcatcgcc
     2581 ggccgcgcgc ctgcgcgtca tccgagagct tgcggacgct gacgtgccgg tcggcatgct
     2641 cctcgcgccg atgatcccgg cactgaacga ccacgagctg gagcgcctgc tggaggctgg
     2701 ccgcgatgca ggggcaagcc gggccggttt catactgctc aggctgccgc gggaactggc
     2761 caccttgttc gaggagtggc tgcgggagca tttcgccgcg cgcgccgacc gggtgctgaa
     2821 cctgttgcgc gacctgcgcg gtgggcggct gaacgacccc cgcttcggcc atcgcatgcg
     2881 cggcgagggg ccgtacgccg acctgctgtc ggcccggttc gacgtcgcgt gccggcgcct
     2941 gggtctggtg accggccgcc gccacgacct ggataccgcg gccttccgga gcgatccggg
     3001 tgctccgcaa cagggcgtgc tgtttggctg agtgagacgc tgttccggcg ggtcccgcgg
     3061 cacccgtggc gtatacttgg atcgccgcat tgcaggcatt ccacacccgc ggccggaagg
     3121 ccgggatgag ccgccgtgac cgttcaagga cgtgcaggac agccgccgcc gagactcgcg
     3181 cgccgagcgc gggcaggcct gttcgcaggc gtgacggccg tcgcggcggc tggctgcatg
     3241 acctccaagg tggacgagac acggcacgtc gcggcggcga tccagcccaa tgaatccatc
     3301 gtgatcctga agaagccgca gctcgagggt gtcggcaccg agggcgagtt cctgagctgc
     3361 gtccaggaaa aactcggcgg cgaactcgtg catccgcagg atggccagtc cgcgaagccg
     3421 gcgtcttcgt cgccggtgcc tttcaagatc tacggcgagc agcagttcga ggacgcgatg
     3481 ttcccgtggt tcgagccgag caccgcgccc gccaacgcgg ccgggatgaa gaccctgctc
     3541 gagcacccgg gcatcaccga gcgcctgacc cagatcggcg tgcgctacgt cgtgtggctg
     3601 gacgggaata cccgcaagac ggacggaggc ggcagcgttg cgtgcgccat cggtccgggc
     3661 ggcggcggtt gtttcggggt cggctggtgg gacaaggaag ccgattatgt cgccacggtc
     3721 tgggacctca acacggccac cgaactcgga accgtcacgg ccgacgtgac cggcacgtcg
     3781 gtcctgatcg gggccgtggc gccgattccg atcatcacgc cggtgcaggg caaggcctgc
     3841 agccggctat ccgagcagct gcgctcgttc ctgaccggca gcgatctcgt ggcgcccgcg
     3901 cccgcggcgg ccacgggcag cggcgggtat tgaacctagc cgcggcaggg cggatccaac
     3961 ggtgagtggg cggcgatcat cgccgcgccg cggggagttc atggacatgc cgaagacgat
     4021 gcatcgcctg ctgtggtccg ccgtgtgcgc ggcgggcgtg gccggctgcg cgtcgagcac
     4081 gtccacgcag tcgtcgtcca tgccgcagat gccgagcccg tcgtcgtcgc cgtcctccca
     4141 gtcttcgcca tcctccggcg gcagcccgag cactggccag ggctcgggtg ccggccagcc
     4201 ttcgagcggc gggcagcctt cggccggtgg tggccagggc tccggcagcg gcggccagga
     4261 gtccccgttc ccgtctcagc gcagccaggg tgggtcgccc tcgagcggcg gnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nacggctccg aggcggcggg cgagaacggt
     4441 gacggcagcg caggcgatgg caccgcgggc ggcgacggca agtcgggcgc ggggacgtct
     4501 ggatccgccg ccgggaccgg tggcgacgac ggcggccgcg gcgagtcggc cggaggcacg
     4561 tcgggcacgc aaggtggcgg tggcggttac ggcggcggtc cgaagggcgg cgccggcccg
     4621 aacaccgtgc ccccggacat ccccgacggg agcgacgacg acatcgtcgc gaggcagctg
     4681 cgcgaagcag cgatgaagga gaccgacccg gagttgcgtg agaagctgtg ggacgagtac
     4741 cggaaataca agcagagcac gcagtgagca gactcgccac atcgcccggc ctccgcgctg
     4801 cgtcgttcgc ggcgatggcc gtgctgctcc tcgcgggctg cgtcgtgcag gacgagcgcc
     4861 cgatgccgcg cgtcatcgcg cagaaggcga cgaccgaggt gccgcaggac gagctgctcg
     4921 acgtgggcgt gcgcctgttc gacccgaacg tcccgaagga cccgaagaag ctcgaggacg
     4981 gcgagatcta cccggaagtc cgcgacgcgg agtcgcgcta catcccggtc gagatccgcg
     5041 acacgctcga aggcaccggc cagtggggcc aggtgcgcgt gctgccgcgc gatgcaaccg
     5101 gcatggacgt ctacgtcgac gggaagatcg tcgagtcgga cggtcgcgag ttgaagctcg
     5161 acgtcacggt gagcgactcc tccggccgcc tctggtacca caaggaatac gagggtgcgg
     5221 ccgacacccg ggcctacaag gacgtcgcgt cgaagccggc ggacccgttc gagaacgtct
     5281 acgccacgct ggccaacgac ctgctcgccg cacgcgcaaa gctgacgcgg gagcagcgcg
     5341 tgcagctgca cgagatcacc gacctgcggt tcgacgcgga cctcgcgccg tacgccttcg
     5401 gccagtacct cgcgaaggac cgcaagggct attacgcgat cacgcgcctg cccgcggccg
     5461 acgacccggt gctgcagcgc atggaccgcg tccgcgagcg cgactacgcg ctgatggaca
     5521 ccctgaacga gtactacacg aacttcgccg agagcatggc cgtgccctac acgagctggc
     5581 gcaaggccac gtacgaggag ctcgaggcgc gctcgaaggc acagcgcgag gcgctgacgc
     5641 gccagatcct cggcgccgtc gccgtggtcg gcggcatcgt ggccggatcg aacagcaaca
     5701 gcagcgcggc ctccgccgcg gcgacggctg cggtgatcgg cggcatgtac gcgttcaagt
     5761 ccggtctgga ccagcgcgcc gagatcagga cgcacaccga gtcgctgaag cagctcggcg
     5821 actcgttcca gtccgaggtg cagccgatgg tggtggacgt cgagggacgg acgctgcagc
     5881 tcaagggctc cgccgagcag caatacgccg agtggcgcca gctgctcaag cagctctacg
     5941 agaacgagac cggcttgccc gcgaccggcg gtacggccgc caacggcacg ccgacggcgg
     6001 cgacgaagcc ctgatcggct gcccacacca gccatgactt tcgagagcgg acaggttctc
     6061 gcggcgcgct acgcgctcga acgcaggctg ggtgacggcg gcacggccga ggtctggctg
     6121 gcgcgcgacg gcgatgcgcg ctgcgtcgtc aaggtactgc gcgcggaacg cgcgcgcgag
     6181 cccgcggaac gcgctcgctt cgtcgccggg gcgcgattgc agcgcgcgac gcagcatccc
     6241 aacgtgctcg cctgcatcga cgtggtcgag tcggaacccc cgttcgccgt attttccttc
     6301 gaatccgcga ccgacctggc cggcatgcgc ggcgcgccat ccaggcagct ggtgccggtg
     6361 ctgtcgcagg tcgccgcggg actgggtgca ctgcacgcga acggccacgt gcaccgtgac
     6421 atcaagtcgt ccaacgtgct catgaccgaa gatgggcgcg tgctgctcgc cgacttcggc
     6481 ctcgcggcgg cgatcggcga tgcgacggcc gcggcgggcg gatctccttt cacgtccagc
     6541 ccggagcagc tcgcgggcag cgcgccgtcg atcgcggacg acgtgtatgg gttcggcgcg
     6601 ctcgcgttcg agctcgcgac cggttacccg ccgttctatc cggacgacgc ggcggcccgc
     6661 acggcggccg agccaccgcg gctcggcgag cggcgcagtg gcgtgccggc ggcgctcgag
     6721 gacctcgtga cacgctgcct ggcacggaat gccgcggatc gcccgcgcga tctgacggaa
     6781 atctcggccg cgctggcgcg catggcagct gcagccgatg cgccggatgc cgggcgaacg
     6841 cgccggccga cggtcgccct gcgcgtgccg gacgcaggtc ccgcggcgat cgagcccagc
     6901 tggcagcggg ccgcatcgac cgggcccagc gccgccgagc tgcgctcgca ggggttccgg
     6961 cgcggcctgc tcgcaggatc gttcgtcttc ctgctggtgg tcgccggtct caccttcttc
     7021 gcgttgccgg actgggtcca acgcaatgag ccggtggtgg cggccccgcc gccggccatg
     7081 cccgcgaagc cggcgcaagc tgcggccacg ccgccggcga aggatccgca ggccgaagcg
     7141 cacagggccc tgcagctgaa gcaggagcac gagcagcagg tcgcggcgca ggtgcgcgcg
     7201 gcgctggccg ccggtgcggc agccatcgac gccggcgatt cggccgaggc gcagcggcaa
     7261 tacggcatcg cggccagcct cgatccgacc aatgcagcgg ctcgacgcgg cctcaaccgg
     7321 gctgggacgc tcgatgaagt gcgcaccctg cttgcgcagg ccgcggaagc cgagcgcacg
     7381 ggccggcttg cggacgcgga acgtgcctgg ggccgcgcgc tcgaactcga tccggacacg
     7441 accgcggcgc gcgaagcgct cgcaagggtc aggtcggagc aagcggccgg agcgttctcc
     7501 gcggccgtcg ctgacggact ggcggcgttg tcgcgcaagg actacgcggc ggcacgtgct
     7561 gcgtacgagc gggcgggtcg cattcgccct ggcgccccgg aagtcaagga cggactggag
     7621 caggtcgacc gggcgctcgg cgaccgcacg atcgcgcagc acctcgatgc cgcgcgcagg
     7681 gcggaaggcg aggagcgctg gcgtgacgcg ctggccgaat accgcgccgc gctcgtgatc
     7741 gatccgaacc tgctcgatgc acagcaaggc gtggaacgcg tcgagcccag ggtcatgctc
     7801 gatgcggcac ttgcttcgta cctggagcgc ccggagcgtc tcttttccag cgacgtgcgt
     7861 ggggcagcac gcgtgacgct ggcacaggca ggcgcgatcg ccagtccggg tccggtgctc
     7921 acgcgccaga tggcacaggt gaccaacctg ctcgcagccg cggagacgcc cgtgcgtctc
     7981 acgatcgcgt cggacaacgt gaccgaagtg acgatctatc gcgtcgggcg actcggcgca
     8041 ttcgagcaca aggacatgga cctgctgccc ggtcgctaca ccgtggtcgg gacacgcgcg
     8101 ggtttccgcg acgtccgccg cgaggtcacg atcatgccgg gcaagcagcc gccgcaggtc
     8161 gtgatccgtt gcgaggagca gatttgagcg tggtactcac actgcgcgag cccctgggag
     8221 agcggacatt cgcggccgcg gatttcccgc tctcggtcgg cgggaccggc agcgacatcg
     8281 tcgtaccggc gcccgcaccc gggccactcg cctggttcgc gctgcacgac gggcaattgt
     8341 tcgtgcagcc ggcggcggac gacgcccaca tcctgcacaa cggttccagc gtgaccgcct
     8401 cgacgtggtt gcgcggcggc gacgtactcg acgtcggggg cggccggctg aagctgcgca
     8461 gcgacccggg tgggcccgtg ctcgacctgc tgtcgggcgg cgacggaaac gtcacggcgc
     8521 ctccagtggt gccggcgtcc gagagcgtcg ccggcgaggg agcggacgag gccgagcgca
     8581 tcgaggcgat tgcgttccgc ctgcaggggc cgcgggctca gcgggccgga tggtctcccc
     8641 ggcgcatcgc cgtcgtggcc gtcgcggcat tgctcgcgat catcgccgcg ctcgtgttca
     8701 cgtccacgcc cgtcgaggtg cagatcgagc cagccgctga ttccgtcgta ttcgaaggga
     8761 cgtggcccgg cctgcgcctg ggcacgagcc acctgctgcg acccgggcaa tacacgctcg
     8821 tcgcgcggcg tgcgggctat gccgagctgc gcgcgcccgt cacagtgagg cgtgagtccg
     8881 ggcaggtgct tcgctaccgg cttgtcgcgc ttcctggtcg tctcaggatc gacctgccgg
     8941 tccccggtga ggtcaccgtc gacgggcgcg cgatgggcaa ggcgcccggc gaattcgaac
     9001 tcccgggcgg aatgcacgag ttgcgaatcc acaccgagcg ctatctggac tacgcgacga
     9061 aggttcgaat cgccggctac ggcaagcggc aggccattgc gccgagcctc acgcccgcct
     9121 gggccgtcgt ccggatgtcg accgagcccg ccggcgcgtc ggtacgcgtc gggggcgaag
     9181 ctcgcggcgt gacgccgctc gagctggagc tcatggcggg ctcgcaccgc gtggaactgc
     9241 tgcacgcggg ctacaagccc tgggtgagcg acgtccaggt gaaggcgaac gagccgctga
     9301 ccatcggtcc cgtgcgtctc gggctgccgg acggccggct cgcggtgcgg agccggccgt
     9361 cgggcgcgag cgtggccatc agcggcgcct accgtggccg cacgccgctc gaggtcgacg
     9421 tgcgccccga tctcgcgcag accgtgtccg tgacgcgcga gggctacgag cccgcgagcc
     9481 aacaggtgac ggtcgggccc ggcaaggccg ccgtcgtcga ggtcgcactc accccgatcc
     9541 tcggcgacgt gatcgtgcgc gcgagccctg ccgacgccca actctacgtc gacggcgagc
     9601 tgcgcggggc accgaaccag acgctgcaac tcacggcgac ccgtcactcg ctcgagatcc
     9661 gcaagcccgg gtacgcgccg tatcgcacga ccgtcacgcc gcggcctggc ttgccgcaga
     9721 gcatcgaggc gacgctgctc gagggcgtga tgcccgcgac ggcatcggcg cagccgtcgg
     9781 ccggggccgc cgccggcgcc acgcccgcga cgacggatgc cgtcgcgctc cagccgatcc
     9841 tgcacacgaa ggccggccag gacctgcgcc tcgtgcccgc cgggacctac acgatgggga
     9901 gcccacgccg cgaagcaggc cgacgcgcca acgagtcgca gcgaccggtg gagctcaagc
     9961 gtcgcttcta cgtcgcgacg cgcgaggtga ccaacgcgga ataccggcag ttccgtcccg
    10021 aacaccgctc gggctacgtg atgcagacca cgctcgatct cgaccgccag ccggtcgtga
    10081 acgtgagctg gcaggaggcg gcggcatact gcaactggct cagcggccag gacggcctca
    10141 cgccggcgta cgagatgaag aacggcgcgt tcgtcacggt cgtgcctgcg acgaacggtt
    10201 atcgcctgcc gacggaggcc gagtgggaat ggatcgcgcg ccgcgacggc ggtggtctgc
    10261 gcaagtatcc atggggagac gcgctgccgg tgccgacggg agccggcaac tacgccgatc
    10321 gttccgccca ggcgctgctc ccggtggtgc tcacggatta cgacgacggc tacgccgcca
    10381 ccgcgccggt cgggagcttc gccgcgaatc cgtccggtta cttcgacctg ggcggcaatg
    10441 tcgccgagtg gacccacgac ctctacactg tgcagccgcg ctccgagacg gcagcggtcg
    10501 acccggccgc ggccggggag ggcgcgatgc acgtcatccg cggatcgagc tggaagagct
    10561 cgtccgtgac cgaactcagg cttgcctacc gcgactacgg ggatggcaga cgcaatgacc
    10621 tcggattccg catcgcgcgt tacgcgcagt aaacgcagtc tcgtggcggc ggtcgcgctg
    10681 ttcgcgggcg ggctcgtcgc ggtgggcgcg ctgcgcgcgc aggagccgca gtcgcccgcg
    10741 cagacgcaac cggcggcgcc gcctgcagag gacaaggcgc agcctccggc atcggcgccg
    10801 ccggcaggcc gcgcgcagcc gacggatggc aagacgccgc ccgcgagccg cggcgcagcg
    10861 gccacaggcc agacccagga acacttcgag cccaccgaga agatccgcgc cgatttcgag
    10921 gtgtcgttcc cggtggacat ctgaacagcg aggacccgac acgcatgccg aggatcaacg
    10981 cgcgcgcccc gagcgaactg tggttcagcg tcttctcgct gatcgtcttc accatcgtcg
    11041 tgcacgcgac ttacgtgacg gtcgtgcggc cgcgggcgag cgcggtgctg gccgagcagc
    11101 gcgccgcgat gaagaaggac ccgaactacg tggcggagcg ctcgatctac gtggtgctca
    11161 aggactggga gcaggaatcg gaggtgatcc tcggcctgtg ggccttcgcg atcatcggct
    11221 acaaggcctg gcactcgcgg cgcgagcgtg ccctgctcgc ccgcgacctc gtgcacgtgc
    11281 cggacggcat gaagatcctg cccgaggaca cccgtgagta cgcgcgcccg ctcgaggcgc
    11341 tgccgccgac ggagtcggag tgcctgctgc cgcgcgcact gctcgtcgcg ctgtaccggt
    11401 tcgcggccac gcgcaacgtg caggacgtct cggatgccgc ccgcagcgtg tgttccgccg
    11461 aggcggaccg gctggactcg gagctgtcca tgctgcgcta catcgcgtgg gcgattcccg
    11521 cgatcgggtt catcggcacc gtgcgcggca tcggcgacgc gctgggcgag gcccacaagg
    11581 cggtgaccgg cgacgtgtcc ggcgtgaccg aggggctcgg cacggccttc aactcgacgc
    11641 tcgcggcgtt gctcatcagc atcgtgctca tgttcctgtt gcaccagctg cagctggcgc
    11701 aggagcgcct ggtgctcgac agcgagactt acctggaccg caacctcatc cggcacatgc
    11761 agctgcgctg agcagatgac actcgcgctc gagatcaacg acgccggact cgtgctggcg
    11821 cgcgacggcg ggatcctcgc cgacgagccg ggcatcgtga tgctggaccg cccgactccg
    11881 gagaccggcg cggccgccgc actgcgcgcg aggctgcaac cgctctacgc cgagacgcgc
    11941 cactggcagg acctcggcac cgagccgctc gcccgacccg tgcccgccgc cgccgatcga
    12001 gccgaggtcg cctacgcgca gcttgcggcc ttcgtcgcgc cgcatctcgc cggtggagcc
    12061 gatacgctcg ttgcggtccc gccgtggtac acgcgcgagc agctgggcct gctgctcggg
    12121 atcgccaggg agtgtggcct ggaggtcgtc ggcctggtgg acgcgggact ggcggccgcg
    12181 tcgctcgcgc cagcggccgg caccatgctg caactcgagc tctacctgca ccgcgcggtc
    12241 gtctcggtgc tcgaccacac gggcggcctg cgacgcacgc gtttcgacct cctgccgcag
    12301 cacggctggc tggggttgca gcaggcgtgg ctggacgcga tcgcttcggc gttcgtgctg
    12361 cggacgcgct tcgacccgtt gcaccaggcc gccaccgagc agcgcctctg cgacgggttg
    12421 cgcggctggc tcgacgcatc gctggcgggc ggcgacgtcg cgatcgagat cgattccggg
    12481 gcaggcccgc aggccatcga cttcccggcc ggcgacttcg tcgcagcggc tgcgcgcacc
    12541 tacgacgagt acgtccgcgt gctgcagcgc gcccgcccgg tcggcggccc gatgcacctc
    12601 aggctgtcgg accgtttcgc gaagctgccg ggcctcgcgg cgcggcttgc ggagatccgc
    12661 gacagcgagc tgcagtggtt gccgcgcggg gcggccgcgc tcggtgcgct cgcctgggaa
    12721 aaacacatcc gccagggcaa tcgctcggtc acgctggtcc agcacctgcc cgtcgaactg
    12781 cagacctcgg cagggattac gccgctggtc gtcgcggcaa gcgtgcccgc ggatgcgcga
    12841 cccacccacg tcgtgcatgc gggccgcgca cacccgctcg gtccgcggcc gctggtgctg
    12901 ggcgccgacg ttccgcccgg ccggcgtgcg ctgcgggtcg cgcccgggcc gggcatctcg
    12961 cgcgcgcact gctcgatcgt gctcgagggc ggcgcggcct ggctcgagga tcacagcacg
    13021 tacgggacgt tcctgaacgg cgagcgggtc ggcggacggg tggagttgcg cgccggagac
    13081 cagctgcgca tcggcagccc cggtgtgact tgcgaactgg tgcgcgcggt cgaggacgat
    13141 ggcgcgacgt aagaccgagg tcttcagcct ctcgttcctg gacgtcatcg cgtgcgggtt
    13201 cggcgcggtc gtgctgttct acacgatcct gagcgcgcag gccggcgtgc agcgccagca
    13261 ccgcaacgac gagctgcagt ccgaggtgaa ccggctcgag gagcaggtcc tcgacggcta
    13321 caagaacctc gtcgtgctgc gcaacgcgct gcagagcgac gagaagcaag ttcccgccga
    13381 ggggctcgcc aaccgcatca tcgaggaaac ggaacagctg cgacagcagc tcgcggaggc
    13441 ggagcaggag acgctttcgc gccgcgaggc gatcgagcga ctgaagcagg acctgaagtc
    13501 gctcgacgag ggcaagcgcc ggctcgaggc cggcacgccc agtcccggcc agccgggcac
    13561 gcgcatcaag ggtttcatcg gcagcggcga ccggcagtac ctcacggggc tcaaggtcgg
    13621 cggccagcgc atcctgatcc tggtggacgt ctcggccagc atgatggacg agtcggtcgt
    13681 caacgtgatc cggctgcgca acatggcgga cgtgcggcgc atggcggcgc cgaagtggcg
    13741 gcgcaccgtg gcgaccgtgg actggctcac gtcgcagctg cccatcaagt cccagttcca
    13801 ggtgtacgca ttcaacaccc aggcgcgccc gctggttccc ggcagcgccg gcaagtggct
    13861 cgccgccagc gacgcggcgg cgctcaacga cgcgctcaag gaattgcgca aggtcgtgcc
    13921 ccaggacggc accagcctcg agaacgcgct ggcggcggcc gggtcgctgt cgccgaaacc
    13981 ggacaacatc ctgctcatca ccgacgggct gcccacgcag ggcgcgtcgc cgccgctggt
    14041 gcgcaagacg atcgacggcg acgggcgact gaagctcttc gagcacgcgc tcggcaggct
    14101 gccgtccggc atcccggtca acgtgatcct gctgccgatg gaaggcgacc cgatggcgcc
    14161 gagcgcttac tggacgctcg cgcgccgcac caacggttcc ttcctcagcc cggcgaagga
    14221 ctggccatga agaactcgcg gctcggccgg cgcgacgtcg aggtgttcag cctgtcgttc
    14281 ctcgacgcca tctgctgcgg cttcggcgcc gtgatcctgt tgctcgtgct gacggaagtc
    14341 ggcgagcccg tggtcatcga gaagtcgcgc gaggacctgc aggcacaggt cctcaaactc
    14401 gagcaggaat tgaacgacat ccggggaacc agcgaggtgc tgaatcgcga gctcaaggcg
    14461 cgacgcagcc agctgtccga ggagcagctg cggatcgccc gcctgcgagg cgacctgagc
    14521 gccttgcgcg gccagtacga atcctcgcgc aaggaggcgg aagtccagaa ccagatcgag
    14581 ggacagctcg tctccgccca gcaggacctc accgaggaga tgaagcgcct gctcggcgcc
    14641 cagtaccggc gcaaggaaga ctcgcccatc ggcggactgc ccgtcgacag cgagtacgtc
    14701 atcttcatca tcgacacctc gggcagcatg gccaactacg agtgggcact gatgcagcgc
    14761 aagctccagg aggtgctcga tgcgtacccg acggtcaagg gctggcaggt gatgaacgac
    14821 gagggcacgt acctgttccc cacctaccgc ggccgctggc tgcccgacac gccgatgcag
    14881 cgcaagctgg tgctcgagcg catgcgcgac tggttcccgt tcagcaactc gagcccggtc
    14941 gagggcatcg tcgaggcgat ccgcacctac tacgcgacgg ggctcaagat cagcctctac
    15001 tacttcggcg acgagttcac cggctcgtcg atcgactcgg tcgtgaggac ggtggacatg
    15061 ctgaatcgcg aggacaagac cgggcagcgg cgcgtgcgca tccacgccct cgggttcccc
    15121 gtgcgcccgg atgcgccgca ggtgacgagc atccgctacg cgacgctgat gcgcatcctg
    15181 tgcgagcgga acggcggcac attcgtggga ttgaacgagc ccgggagcta tgcgcgctga
    15241 ccgccatgat gcgaccatga gacggatggc cacagcagcc caggcccggg ttccgcgtca
    15301 cggtccggat ggccgtaagg cgtcgccgcg cgcgccgcgt tcctggctgc gccgtgcatc
    15361 ctgggccgcg ctcgcgctgc tcgcgggctg cggcacgacc gagttcgagg ccaagccgac
    15421 catacccgag ccgctcgtca cgaagatccc gctggtggtc ggcgtgtacg tcccggccga
    15481 gttccgcgac aaggtctaca aggagaagcg tgacggcggc gactactcca ttgcgatcgg
    15541 caaggcccag tccgaggcat tcatgcgtct gatgggcgca ctgttcgagc gcgtgattcc
    15601 agtggatgca gccgatgcgg gcgcgcgcac cgacccgcag atccgcggtg tcgtcgagcc
    15661 cgtgctcgag gacgtcgcat tcgtcacgcc ggtcgaatcc ggcaccgatg cgtacgcggt
    15721 gagcctgcgg taccgcatca acggctaccg cccggacggg caggtcatcg actcctggat
    15781 cttcaccggc tacggcgcgg ccgccagcga gagcatgctc ggcggcggcg ccgaggtgct
    15841 gaagaaggcc ctgcacctcg cgatgcgcga cgccgcggcc aagctcgcca cggaattccg
    15901 cgagcaggcc atgatccgtg gactgctgcc ggcggaatcc acgccgcagg ccccggtcga
    15961 ggtggcgccg cctccgccct gaccgttcaa ccgccggggt tggcgtcctt gtccgtcgac
    16021 ttgtcttcgt cgacgagcac gtagaaccgc tggtccagcg tnnnnnnnnn nnnnnnnnnn
    16081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    16141 nnnnnnnnnn nnnnnnnnnn nccttgtccg tcgacttgtc ttcgtcgacg agcacgtaga
    16201 accgctggtc cagcgtcacc cccggcgtgt cgacggccac accgttctcg aagcgggggc
    16261 ggtagatggc cgtctggaga gcgcgttgca cgctggtccc gtgactcggg tcgccggcgt
    16321 cctcgagcac cttcgcgtcg gcgacccgcc cgcgcgaggt caccgtgaat cggacctcca
    16381 cgttgcgcac gacgacctcg tcgggcgagc gtgacgcgaa gcgagtcgcc gcggtcggcg
    16441 gcacgtacgc gatcagcacc ggcttgccga acagcaggtc cgtcagctgc ctgccggcca
    16501 cggtcgccgt tcccgcggcc cgccaggcga gttcgtaatt cgcagcggcc ttgtccggca
    16561 gttgccgcga ctggtaccag tcgccgagca gcgtgcgcgt gtcggcgacc tgcgcaggcg
    16621 acggtggatc gagctgggtc gcaatggcga ccgcgcgttc cagcgaccgc tggccatcgg
    16681 acgtgacgct ggtcgccgac atggccgtcg tcaccatcgg cagcgactcc ccgaacatgg
    16741 ggcgcggcgg ctggtccggc gacggggcca tggaggacgg atcgagcaac tggaggcgtg
    16801 cgcactcgcc cagcgccgtg agtggcccga tgagttcgcc ggcgttctcg cccttctgac
    16861 cctcgatgat gccgatcgac tgccgcagcg cggtgcgggc cgcatcgtac gagccgacgt
    16921 gcatcaacca gcgcccggcg gactccagcg cctgcgcgta ctggacgctc gaggcgccgt
    16981 attttcgctc gacggtgcgc aggatgtagc ggcgcacctt gagtgcgtcg ctcacgcggt
    17041 cgagttcggt gagcgcgctg gcgtatttct cgaggtatgg cagctgctcc tcggtgaaca
    17101 gtccctgttc gcgccggacc agggcgatgg cctgctcgta gcgcgccgcg gccaggtcgt
    17161 agcgcttgcc tgcgtagtac gtggtcgcga ggcccgcctg ggcacgcgcc aggcgcgcag
    17221 tggtccggcg gccggaatct tcgatgagct tgatgacgcg caggtagctg tcctcggcgc
    17281 cgaggtagtc ctcgtcgaag tactgcgcca gcgcgaggtt catcagcgcc acctgcaggt
    17341 agtcgccgcc gggtcccggc tgctgctccg ccgcggcaag ctgctgccgc gcctgcgcaa
    17401 ccgcggcgtc gtactgcttc gcatccagca actggcgaac gtcggcggcc ggcacggtgc
    17461 tcgccggcgc cggctgcgtc tgggcaaggc acgcctggct gcagattgcg agcagggtgg
    17521 ccgagctcat cgcgagccag aggcgggacc gatttcgtga ggtcaggacg tccaagccct
    17581 gcaacaacta cacggcgggc gcccgtgatg caatcccgct caaccggtcc agcgcatccc
    17641 gcgcgccggg cgctgcaccg ccatcgtctc caccgtgtag cgccacgact cgacgtccgc
    17701 ggcccagaag tccgcgtccc agcctgcctt ggccttgagg tggcgtatga attcccccgg
    17761 gtcgggcagc tgttcccaga ccttgggcag gaacgtggct cgctggcccc gccacgtcag
    17821 cacgagcccg tcgacgcaag gccgcaaccg ccgttgcagc gactgctcgc tgccagcttc
    17881 cactcgttcc agtggcgaca gcaccgagat ctccagcgac gcctcctccc attcggagcg
    17941 cgcaagcggc gcaaagcgcg gatcgccgaa ggccgatgca cgggcgtttt gccacacgtc
    18001 gaaggcgagc gcccgtcgcg gctcgagcgt cccgcagcag ccgcgcagtc gatgctcgac
    18061 ggtcaacgtc acgaagctcg cccgctgcag ctgcaattcg gcgggcagtg ccgccgtggc
    18121 cggaggctcg tccggtgcgc gcgtgaggcc ggtctcgatg gactgcgcgg cgatgcgcag
    18181 caacagtccg cgcgcgaccg ggtcaagcgg cgtcgagggt gtagctgcca tacccgacga
    18241 ctcgcgaccg gtcaccggcc gtgtcacccg agttgcgctg gtcgagca
//
LOCUS       DAOMFD010000024        17803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_628_length_17803_cov_33.718447, whole genome shotgun sequence.
ACCESSION   DAOMFD010000024 DAOMFD010000000
VERSION     DAOMFD010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17803
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_628_length_17803_cov_33.718447"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..623)
                     /locus_tag="PL152_02755"
     CDS             complement(<1..623)
                     /locus_tag="PL152_02755"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQR48226.1"
                     /translation="MLPALASPPDRSPGKSGFRLALCTLLLALAACTSLQPRPELPVE
                     SAIPAGTGTRLDDLIAPAEAAHPGESAFRLVSDGAEAYVIRSRSALIAERSLDVQTYI
                     WNADGTGQVLAYRVLQAADRGVKVRLLVDDMDARSKNYGFAALAAHPNIEVRMFNPFQ
                     SRTGTMALLFEAMGSFERINRRMHNKSWIADNRIAVVGGRNLGDEYFG"
     gene            complement(672..2012)
                     /locus_tag="PL152_02760"
     CDS             complement(672..2012)
                     /locus_tag="PL152_02760"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQR48227.1"
                     /translation="MKPLRTLALLSLGLALATQSVAGVTGKAVRASVEGMDVVIVPTD
                     VKDVVTITGSMRAGDDRSPPNNVAIATLTGSMLDKGTTTQDKFAIAQKLGGVGAALHF
                     SVGASALQISGKCLRKDLPLLTSLLAEQLRQPAFPEAELDKLKKQLDGAMRQQMEDTD
                     FRAGDAFSRAVFPPGHPNRQPVPDAFIADVNKAAVDELRQFHAQYYGPAAMRLVIVGD
                     VDPAATIDDLRKAFSGWTGGSVPPPVPDAPPLAAAKVERVAMPDKTSVSVVMGQPTQL
                     RYSDPDALALRMGTRILGSGFTGRLMSNVRDKEGLTYGIGSVVSDDTYSDGDWSIYAT
                     FAPPLLVQGVASAKRQLALWHDQGVTAAELARSKTRVAGSYKVGLATTSGLAGTILLM
                     LDSGMPLEFVDEYPRRVEALTLEQVNAAIRKHIDPDKLVLVQAGTLPGAAGSSR"
     gene            complement(2042..3430)
                     /locus_tag="PL152_02765"
     CDS             complement(2042..3430)
                     /locus_tag="PL152_02765"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HQR48228.1"
                     /translation="MKHIVATICLALVSAVGTASAATTPASAPGFKYVRTVGDISEYT
                     LESNGLTVLLMPDHSAPVVTFMVTYRVGSRNEVTGTTGSTHLLEHLMFKGSPAYNDAK
                     GNSVKQYLETVGGMYNATTWLDRTNYFAVVPRDALAGYMAIEADRMRNLWLRESDRER
                     EMTVVRNEYERGENEPQEALDKAIIAAAFEAHPYHHSTIGWLSDIENVPIEKLRAFYD
                     TYYWPNNATVSVIGDFEPADALRLVKQYYGVYPRSPQPIPQVYTVEPEQQGPRRVTVN
                     RAGEIAVVGIAYKSPAGRDADYAALDVLGTILSNGKTSRYYKALTDRNLALDVDASAG
                     FYHDPSLFQVYAQVAPGVDQAEVERVLVEEIERVKKDGVTQEELATALAKYDAATAYA
                     RDGSFAIASVLNEAIAAGDWTLYYTLDDATHKVTTGDVQRVANKYLVADHATTGWFVP
                     AAPDAAEPASGH"
     gene            3558..4886
                     /locus_tag="PL152_02770"
     CDS             3558..4886
                     /locus_tag="PL152_02770"
                     /inference="COORDINATES: protein motif:HMM:NF012593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQR48229.1"
                     /translation="MADIDARLANRILAALVIGAVAGAVVLAVGSVAPQVLAGAQWLS
                     TALFDPIGQVFLRMLFFVIVPLVFASLAGGVVQLGELSHLGPLAGRTFTLFFLNMAIA
                     VTLGLVMMNTLQPGRHLGEDTSRQLIAEYSGQAARHVEVGATRPEVTPATVVDMFMPK
                     NLFGAFVGNQRGQLGEMLPLILFAILVGGAGLGLPEQRRHSLQRGLETVTELMTGIVR
                     FALRLAPVAVPAMIFSVIVKVGFDIVIALGLFVVGCVAMLMLHLFGTMSLWLRFLAKR
                     RPGEFFRQIKDVLVTAFSTSSSGATLPAALAAARDRLGIAPSTAGFVLPLGTTMNMSG
                     TALYEGCVVLFVAQVFGVHLDIAQQATLVFLAVLSAVAVAAVPGGSLPLIAGLLVAFG
                     IPPEGIGIILGVDRILDMTRTVTNVGADLVTTAVVDAGQRRQYGGPDPAA"
     gene            4986..5906
                     /locus_tag="PL152_02775"
     CDS             4986..5906
                     /locus_tag="PL152_02775"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR48230.1"
                     /translation="MDALRNILVLMEPATETQPAFDAALALARPFGSRLELLISEFQD
                     LHAAYFDPMTATVQQFHDAVLAGHRAALERYEKAASQQGVSALGEAIWGTPFHEVVLA
                     RVAATRPDLVVKHSTFHNRIERTLFTGSDWHLIRDCPVPLLMVKDPARLAGAPILVCV
                     DPLHAHDKPAALDRQLLASAAELSVKLGAELHALNVCAIPAPIGVVGDAYAAAAGMFP
                     VEDWVERSREAYARLLQGYPVGKEHAHFRVGVAARDIVDVARTLHAGLVLMGAVSRRR
                     LDRWIVGSTAESVLDRLPCNVWVEKPAAVS"
     assembly_gap    6049..6148
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6149..7071)
                     /locus_tag="PL152_02780"
     CDS             complement(<6149..7071)
                     /locus_tag="PL152_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48231.1"
                     /translation="MSDTGGNLIERLLSPDAYPHAVEIPIRVAETHISWVLLTGPYAY
                     KIKKPVRLSFLDYSSLELRRTCCEEELRLNRRYAPDLYLAVAAIAGPASAPRVDGAAQ
                     PIEYAVRMRQFDQRQELDALVASDAVGVREIAALGSHIARFHAGARPVDPATPFGRPD
                     AVHRFTTANFAELRRLPEAQRWTARLQDLERRVTSTFDEHRDFIAARRSDDRVRECHG
                     DLHCGNVVRWSGELTPFDGIEFDPALRFIDVANDLAFLTMDLSERGRDDLRHAALQAW
                     AAALGDFAAVRVLPYFEAYRALVRAKVAALRA"
     gene            complement(7097..>8164)
                     /locus_tag="PL152_02785"
     CDS             complement(7097..>8164)
                     /locus_tag="PL152_02785"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF009131.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HQR48232.1"
                     /translation="AAMRFTGDVDAMPEGTVFFVDEPILRVTAPLPEAQFVESRVINL
                     LHFQSMVASKAARCRLAAGPRMLVDFGMRRAHGAEAALLAARACYVAGFDATATVEAG
                     RRFGIPISGTMAHSFIQAHDDEAAAFRNFAGIHPRGLTLLIDTYDTLRAARQVVAMVQ
                     AGVRVDAVRLDSGNLGDLAREVRRILDEGGCGRTRIVCSGNLDEFAIARLLASGAPVD
                     SFGVGTRLDVSADAPYLDCAYKLQEYAGRARRKRSPGKATWPGPRQVFRTLDERGRIA
                     SDVVGVASETLPGRALLQPAMRAGKVVARPELAEIRKHARRELETLPDDCALIDGPVP
                     VVAGISTELRELAASVDRQFP"
     assembly_gap    8165..8264
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8265..8800)
                     /locus_tag="PL152_02790"
     CDS             complement(<8265..8800)
                     /locus_tag="PL152_02790"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HQR48233.1"
                     /translation="MRNPGPGDALLVVDVQVDFMPGGALGVGRGDEIVAPINRLVDRW
                     CAAGLPVFLTRDWHPPNHCSFVDRGGPWPVHCVAGSEGARFSPALRIPGDARVVSKAT
                     RPDEEAYSAFHGTELADRLRQARVKRVFLAGLATDYCVRASTNDARATGFEVVVLTDV
                     VRAVDVQPGDGQRALDEMA"
     gene            9005..9358
                     /locus_tag="PL152_02795"
     CDS             9005..9358
                     /locus_tag="PL152_02795"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQR48234.1"
                     /translation="MTIPVQITFRHMETSQAVETRVRELASHLGVFSDRITSCRVVVD
                     SPHRHHHQGKVYNVKVQLALPGEDVVVDMERPMRDGHEDVYVVIRDAFDAAKRQLQQR
                     MSSLRNEEKRRETLS"
     gene            9426..9821
                     /locus_tag="PL152_02800"
                     /pseudo
     CDS             9426..9821
                     /locus_tag="PL152_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014058.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
     gene            9835..11265
                     /locus_tag="PL152_02805"
     CDS             9835..11265
                     /locus_tag="PL152_02805"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HQR48235.1"
                     /translation="MNLEGTRRDSEYRWVIEPTGPMRVPAVLYADDELIRGMDDKVRD
                     QVCNVAALPGIVGAAYAMPDAHWGYGFPIGGVAAFDADQGGVVSGGGVGFDISCGVRT
                     LLTGLQRDDVLACQRKLADELARAIPAGVGSTGRLHLSEDEMDAMLTDGARWAVARGY
                     GSEADLARIEEQGCISGARPAAVSQRARKRQRDEMGTLGSGNHYLEVQWVAEVHDAAI
                     ATGFGLAPGDVVVSIHCGSRGLGHQIGTDYLRDMAIQAPSFGIRLPDRELACAPIASE
                     LGQRYLGAMRAAMNCALANRQVLTHLCRESFRRILPRGRLDILYDVSHNICKEEEHEV
                     GGSRRRLHVHRKGATRAFGPGSAAIPEAMRRFGQPVLIGGSMGTASYVLAGAATGEAH
                     AFGSACHGAGRSMSRHQATRQWTGGQLVRELAGRGILIRSPSMRGVAEEAPGAYKSVE
                     RVVDVAARAGLAHKVARLEPLVCVKG"
     gene            11270..12262
                     /locus_tag="PL152_02810"
     CDS             11270..12262
                     /locus_tag="PL152_02810"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQR48236.1"
                     /translation="MIDALLFAPSASRAFAERVASALGTSLSPMEEREFEGGEHKTRP
                     LVSVRGRRVFVLQSLSGDAAGSANDRLCRMLFFLAGLKDSGAAHVTACVPYLAYARKD
                     RRTKDRDPVTLRYVAQLIEAVGTDHVIGLDAHNLAAFESAFRCPVDHLEAAPLLATWL
                     AQQAGDAPVCIVSPDIGGAKRAQRVQDLLESRTGREVGFALIEKRRSSGVVSGGEQVI
                     GPVAGTRAVIVDDLISAGTTLMRGVRACRAAGAVAVDAFATHGVFVPAASQLLGPDGP
                     DRLIVTDSILPTRIGSEARAPIVLPVSQLYAEAIRRICSGESVVALRELQPTRC"
     gene            complement(12281..13306)
                     /locus_tag="PL152_02815"
     CDS             complement(12281..13306)
                     /locus_tag="PL152_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48237.1"
                     /translation="MSDVLTQPSPASPNEPASLADKVAHLKRLVGSGDATIETHFAWI
                     FLVGDRALKLRKPVRRDTMDYSTIAARHADAEDDVRLNRRLAPGVYLGIVPLVRRADG
                     TLTAGGPGETVDWLVEMRRLDRTLFLDAMLARGAVRPAALDEVAGLLAGFYAAAEPAI
                     TGPGQLGARLAAQVAANALAIESLDAAKSRGLATLQRAAIAALAAELDARARAGCVVE
                     GHGDLRPEHVLLSSPPLIIDCLEFDRDLRILDRAEELCVLELECARSGHAAAGRRLLE
                     SCLAKLGDEPSARLLDLYRSHRAARKAKLYIWRSAEPDGGTPDEWRARAADYLETALA
                     AALRAAH"
     gene            13412..14086
                     /locus_tag="PL152_02820"
     CDS             13412..14086
                     /locus_tag="PL152_02820"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HQR48238.1"
                     /translation="MERVFEDRVEAGRALALALGRRHDLQGAIVLGLPRGGVPVAFEI
                     AQALGLPLDVLVVRKLGLPWQPELAMGAIASGGAVVLNEDVVRLAGRDDKVLEQVQQQ
                     EFAELARREHAFRGSRPPLAVKGRAAIVVDDGLATGATMEAAVRALRALGALRVVVAV
                     PVASVEARERIQAVADEVVCLEAPWRFSAVGQWYRDFAQTSDAAVTELLARASLGTTA
                     DPRSRT"
     assembly_gap    14142..14241
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <14242..14618
                     /locus_tag="PL152_02825"
     CDS             <14242..14618
                     /locus_tag="PL152_02825"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR48239.1"
                     /translation="ELGPEVIKVDEFSGREEALGLDPARLVPGSPMPDQHVRNLYTDD
                     TGRFFGGIWRSGKGAWHVSYTENELCVLTEGRVRISDDHGRTWTFGPGDCFVMPAGFR
                     GVWEVLEPARKFYAIYEPPPPG"
     gene            14851..15597
                     /locus_tag="PL152_02830"
     CDS             14851..15597
                     /locus_tag="PL152_02830"
                     /inference="COORDINATES: protein motif:HMM:NF021230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TorF family putative porin"
                     /protein_id="HQR48240.1"
                     /translation="MKLTRGLAMVALLATGATAQAVDLSATITGVTDYDFRGITQSAQ
                     NPALQGSVDLATDAGFYAGIWGSNIDFGPGDPNAEVDWYAGWGGGEDLTWDLGITYYT
                     YINGGSFNYPEAHLGFGYKWVDVKAWYGWDYGGIDGAEEHYYETNINYPLPANFGLAG
                     HIGYSNGDGIKASTGQGSYMDWSVGVTYTWSHFDMSLKWVDGSDAKYLDPPYLYKDNC
                     DCFKKTDVFSSEARAIFQISTTFPWKKEGE"
     gene            complement(15711..17039)
                     /locus_tag="PL152_02835"
     CDS             complement(15711..17039)
                     /locus_tag="PL152_02835"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HQR48241.1"
                     /translation="MSAQHPLDRAELEAFWMPFTANRQFKAKPRLLAKASGMYYWTPE
                     GRQVLDGAAGLWCVNAGHGRKEITEAVSRQIAEMDFAPVFQMGHPGAFELANEVVKWL
                     PRGLDHVFFTNSGSESVDTALKIALAYHRVRGEGTRTRLIGRERGYHGVGFGGISVGG
                     MVNNRKFFAAQLPGVDHLAHTHDLAHNAYTRGEPSWGAHLADELERIVALHDASNIAA
                     VIVEPVAGSTGVLIPPKGYLQKLRDICTKHGILLIFDEVITGFGRIGAPFAAEYFGVV
                     PDMITMAKGLTNGSIPMGAVAATKEIYDAFMKGPENAIELFHGYTYSAHPIACAAGLA
                     TQQVYRNEGLLTRAKEIGDYWADAVHSLKGLPHVIDLRNLGLIGAVELEPIAGKPGAR
                     GFDVFVRAFEKGLLARVTGDIIALSPPLIIERKHVDELIGKLAEAIQEAA"
     gene            17166..17723
                     /locus_tag="PL152_02840"
     CDS             17166..17723
                     /locus_tag="PL152_02840"
                     /inference="COORDINATES: protein motif:HMM:NF019498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR48242.1"
                     /translation="MADLTLDVGAHLRAVRTLYGLSQRELAKRAGVTNGLISLIEQNR
                     VSPSVSSLKKVLDGIPMSIAEFFTLDLSASPQVFFRADELTDLGDRSVALRLVAARRA
                     NRAMSVMHERYAPGADTGADMLRHEGEEGGVVVRGRIELTVGGETRLLGPGDAYYFTS
                     AIPHRFRNSGSEPCEIVSASTPPTF"
BASE COUNT         2627 a         5882 c         6178 g         2816 t
ORIGIN      
        1 ccgaagtact cgtcgccgag attgcggccg ccgaccaccg cgatccggtt gtccgcgatc
       61 cagctcttgt tgtgcatgcg ccggttgatg cgctcgaagc tgcccattgc ctcgaacagc
      121 aaggccatcg tgccggtccg cgactggaac gggttgaaca tgcgcacttc gatgttgggg
      181 tgcgccgcca gtgccgcaaa gccgtagttc ttggaccgcg cgtccatgtc gtcgaccagc
      241 agccgcacct tcacgccacg gtcggcggcc tgcagcacgc gatacgcgag cacctggccg
      301 gtcccgtccg cgttccagat gtaggtctgc acgtcgagac tgcgttccgc tatgagcgcc
      361 gaccgcgagc ggatcacgta ggcctcggcc ccgtcgctca ccaggcgaaa tgccgactca
      421 cccggatggg ccgcctccgc cggcgcgatg agatcgtcga gccgggtgcc ggtgcccgcg
      481 ggaatcgccg actcgaccgg gagttccggt cgcggctgaa ggctcgtgca ggcggcaagt
      541 gcaaggagca gcgtgcacag ggcgagtcga aagccagact tcccggggga ccgatccggc
      601 ggcgatgcga gtgcgggcag catttggacc cttccggcag tgcgtccagc gcgcttgcgc
      661 accgcacgag cctaccgtga actgccggca gcccccggca gcgttcctgc ctggaccagc
      721 accagcttgt ccgggtcgat gtgcttgcgg atcgcggcgt tgacctgctc cagcgtcagc
      781 gcctcgacac gccgcggata ctcgtcgacg aactcgagcg gcatgcccga gtcgagcatc
      841 agcaggatcg tgccggccag gccgctcgtg gtcgccaggc cgaccttgta cgagccggcc
      901 acccgggtct tcgagcgcgc cagctccgca gccgtcactc cctggtcgtg ccacagcgcg
      961 agctgcctct tcgccgaagc cacgccctgg acgagcaggg gcggtgcgaa cgtcgcgtag
     1021 atcgaccagt cgccgtccga gtacgtgtcg tcggaaacca ccgagccgat gccgtacgtc
     1081 aggccttcct tgtcacggac attggacatc aggcggcccg tgaagccgct ccccaggatc
     1141 cgcgtgccca ttcgcagcgc cagcgcatcc gggtcgctgt agcgaagctg cgtaggctgg
     1201 cccatcacga cgctcacgct ggtcttgtcc ggcatggcaa cgcgttccac cttcgccgcg
     1261 gcgagcggcg gtgcgtcggg caccggcggc ggaaccgagc cgccggtcca tccggagaat
     1321 gccttcctca ggtcgtcgat cgtcgcggcg ggatccacgt cgcccacgat cacgagccgc
     1381 atcgcggccg gaccgtagta ctgcgcatgg aattgccgca gctcgtcgac ggccgccttg
     1441 ttcacgtcgg cgatgaacgc gtcggggact ggctgccgat tgggatggcc cggcgggaac
     1501 accgcgcgcg agaaggcgtc accggcgcgg aagtccgtgt cctccatctg ctggcgcatg
     1561 gcgccgtcga gctgcttctt cagcttgtcg agctcggcct ccggaaacgc gggctgacgc
     1621 aattgctcgg cgagcagcga ggtcaggagc ggcaggtcct tgcgcaggca ctttcccgag
     1681 atctgcagcg cgctcgcgcc gaccgagaag tgcaacgcgg cgccgacacc gccgagcttc
     1741 tgggcaatgg cgaacttgtc ctgggtcgtc gtccccttgt cgagcatgga cccggtgagc
     1801 gtcgcgatcg cgacgttgtt cggcgggctg cgatcgtcgc cggcccgcat cgaccccgtg
     1861 atggtcacga cgtccttgac gtcggtcggg acgatcacga cgtccatgcc ttcgacgctg
     1921 gcgcgcacgg ccttgcccgt gacgccggct acggattggg tggcaagtgc gaggcccagg
     1981 ctcagcaggg cgagagtgcg tagtggcttc atcggctggt atcccgtctg gatgccggcg
     2041 gtcagtggcc ggacgccggt tcggcggcgt ctggagccgc tggtacgaac cagccggtcg
     2101 tggcgtggtc ggcaacgagg tacttgttcg cgacccgctg cacgtcgccg gtcgtcacct
     2161 tgtgcgtcgc gtcgtcgagc gtgtagtaga gcgtccagtc gccggcggcg atcgcctcgt
     2221 tcaggaccga ggcgatggcg aacgatccgt cgcgcgcata tgcggtcgcg gcgtcgtact
     2281 tggcgagcgc cgtcgcgagc tcctcctggg tgacgccgtc cttcttcacc cgctcgatct
     2341 cctcgaccag cacccgctcg acctcggcct ggtcgacgcc cggagcgacc tgcgcataca
     2401 cctggaacag cgacgggtcg tggtagaacc cggcgctcgc gtccacgtcg agcgcgaggt
     2461 tcctgtcggt gagcgccttg tagtaacggc tggtcttgcc gttgctcagg atcgtgccga
     2521 gcacgtcgag cgcggcgtag tccgcgtcgc ggcccgccgg gctcttgtag gcgatgccga
     2581 ccacggcgat ctcgccggcg cggttcaccg tcacgcggcg cggcccctgc tgctcgggct
     2641 cgaccgtgta gacctgtggg atcggctgcg gcgaacgcgg gtacaccccg tagtactgct
     2701 tgaccagcct gagcgcatcc gcgggctcga aatcgccgat caccgacacg gtggcgttgt
     2761 tgggccagta gtaagtgtca tagaacgcgc gcagcttttc gatcggcacg ttctcgatgt
     2821 cgctcagcca cccgatcgtc gagtggtggt acgggtgggc ctcgaaggcg gcggcaatga
     2881 tcgccttgtc gagggcctcc tgcggttcgt tctcgccgcg ctcgtactcg ttgcgcacca
     2941 cggtcatctc gcgctcgcga tcggactcgc ggagccacag gttgcgcatg cggtcggcct
     3001 cgatggccat gtagcccgcg agcgcgtccc tgggtacgac ggcgaaatag ttcgtgcggt
     3061 cgagccaggt ggtggcgttg tacatgccac ccaccgtctc gaggtactgc ttgacgctgt
     3121 tgcccttcgc atcgttgtag gcgggggagc ccttgaacat caggtgctcg aggaggtgcg
     3181 tggatccggt cgtgcccgtc acctcgttcc gcgacccgac ccggtaggtg accatgaagg
     3241 tcaccaccgg agcggaatgg tccggcatca gcagcacggt gagtccgttg gactccagcg
     3301 tgtactccga gatgtcgccg acggtgcgga cgtacttgaa tcccggtgcg ctggccgggg
     3361 tggtagcggc actcgcggtg ccgacggcgc tgaccagcgc caggcagatg gttgcgacga
     3421 tgtgcttcat gtggggctga ggggtccggt gcagacgcgc aagcttaccc caagggcggc
     3481 ggggatctcg ccggacttgc gcaccggcag ggggattcga caaagtgccg tctcatttgg
     3541 ttcacgaggt ggcccgagtg gccgacatcg acgcccggct cgccaaccgc atcctcgcgg
     3601 cgctcgtgat cggagcggtc gccggggccg tggtcctcgc ggtcggcagt gtcgcgccgc
     3661 aggtgctggc cggcgcccag tggctctcga ccgcgctgtt cgacccgatc gggcaggttt
     3721 tcctgcgcat gctgttcttc gtgatcgtcc cgctggtgtt cgcgtcgctg gccggcggtg
     3781 tcgtgcagct cggggaactg tcgcacctcg gcccgctcgc gggtcgcacg ttcacgctgt
     3841 tcttcctgaa catggcgatc gcggtgacgc tcggcctcgt catgatgaat accctgcaac
     3901 cgggccggca cctcggcgag gacacctcca ggcagctcat cgcggaatac agcgggcagg
     3961 cggcgaggca cgtcgaagtc ggagcgacgc ggccggaggt cacgccggcg accgtcgtcg
     4021 acatgttcat gccgaagaac ctgttcgggg cgttcgtcgg caaccagcgc ggtcagctcg
     4081 gcgaaatgct gccgctcatc ctgttcgcga tcctggtggg cggcgccggc ctcggcctgc
     4141 ccgagcagcg ccggcattcg ctgcagcgcg gcctcgagac cgtcaccgaa ctcatgaccg
     4201 gcatcgtgcg ctttgcactc aggctcgcgc cggtcgcggt cccggcgatg atcttcagcg
     4261 tcatcgtcaa ggtcgggttc gacatcgtaa tcgcgctcgg gctgttcgtc gtcggctgcg
     4321 tcgcgatgct gatgctgcac ctgttcggca cgatgtcgct gtggctccgg ttcctggcga
     4381 agcgcaggcc cggcgagttc ttccggcaga tcaaggacgt gctcgtcacg gcgttctcca
     4441 cgagttcgag cggagccacg ctgccggccg cgctcgccgc ggcgcgcgac cggctcggca
     4501 tcgcgccgtc gacggcggga ttcgtgctgc ccctcggcac gaccatgaac atgagcggca
     4561 cggcgctgta cgagggctgc gtcgtgctgt tcgtcgcgca ggtgttcggg gtccatctcg
     4621 acatcgcaca gcaggcgacg ctggtgttcc tcgcggtgct ctccgcggtc gcggttgccg
     4681 ccgtgccggg cggctccctc ccgttgatcg cgggcctgct cgtggcgttc gggattcctc
     4741 ccgaaggcat cggcatcatc ctcggcgtgg accgcatcct cgacatgacg cggaccgtga
     4801 ccaacgtcgg ggcggatctc gtgacgaccg cggtcgtcga cgccgggcag cgtcgccagt
     4861 acggcggacc ggacccggcg gcctaggtac atcccgcgcc aggacggcga cacgcgcgag
     4921 catcgccggc cgtatcctaa ttcggcgggc ggagccggat ccgccgcatg cctgcggagg
     4981 tgcccatgga cgcactccgg aacatcctgg tgctgatgga gcctgcaacc gagacgcagc
     5041 cggcgttcga cgcggcgctc gcgctcgcca ggccgttcgg cagccgtctc gagctgttga
     5101 tcagcgaatt ccaggacctg cacgccgcct acttcgaccc gatgacggcg acggtgcagc
     5161 agttccacga tgcggtgctg gcgggtcatc gtgcggcgct cgagcgctac gagaaggcgg
     5221 cttcgcagca gggcgtcagt gcgctgggcg aggcgatctg gggcacgccg ttccacgagg
     5281 tcgtgctcgc gcgcgtggcg gcgacccggc cggacctcgt ggtgaagcac agtaccttcc
     5341 acaaccgcat cgagcggacg ctgttcacgg gttccgactg gcacctgata cgcgactgcc
     5401 ccgtaccgtt gttgatggtg aaggacccgg ccaggctggc gggcgcgccg atcctggtgt
     5461 gcgtggatcc cctgcatgcg cacgacaagc cggcggcgct cgatcgtcag ttgctggcgt
     5521 cggccgcgga gctgtcggtg aaattgggag cagagctgca cgcgctcaac gtgtgtgcga
     5581 tccccgcgcc catcggggtc gtcggcgacg cgtacgcagc ggccgccggc atgttcccgg
     5641 tcgaggactg ggtcgagcgt tcccgcgagg cgtatgcgcg cctgctgcag ggataccctg
     5701 tcgggaagga gcatgcgcac ttccgggtcg gcgttgcggc gcgcgacatc gtcgatgtgg
     5761 cgcggacgct gcacgcgggc ctggtgctga tgggcgcggt gtcgaggcga cgcctcgatc
     5821 gctggatcgt cggcagcacc gccgagtccg tcctcgatcg cctgccgtgc aacgtgtggg
     5881 tggaaaagcc tgcggcggtc agctagtcgg ccggaatcct cggcggcgga gttccgccac
     5941 gcacgcagcg acggcatcgg gcgccgacgt gtccacgtcg agcacgtgag cggcctcgct
     6001 cgcggcgaac ggctcccagt aatcgacctg gcgctcgagc gtggcaacnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6121 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ccgcagtgcc gcgaccttgg cgcgcacgag
     6181 cgcgcgatag gcctcgaaat acgggaggac ccgcactgcc gcgaagtcgc ccagggccgc
     6241 ggcccaggcc tgcagcgccg cgtgacgcag gtcgtctcgt ccgcgctcgg agaggtccat
     6301 ggtgaggaac gcgaggtcgt tcgcgacgtc gatgaaccgg agcgccggat cgaactcgat
     6361 gccgtcgaag ggggtgagtt ccccggacca gcgcacgacg ttgccacagt gcaggtcgcc
     6421 gtggcactcg cgcacccggt cgtcactgcg gcgcgccgcg atgaagtccc ggtgctcgtc
     6481 gaaggtcgac gtcacccggc gctcgaggtc ctggagccgt gccgtccacc gctgcgcttc
     6541 gggaagccgg cgcagctccg cgaaattggc cgtcgtaaac cggtgcacgg cgtcgggtcg
     6601 gccgaagggc gtcgccggat cgaccggtcg cgcgccggcg tgaaagcggg cgatgtgcga
     6661 cccaagtgcc gcgatttccc gcacgccgac cgcgtcgctg gcaacgaggg cgtccagttc
     6721 ctggcgctgg tcgaactgcc gcatccgcac cgcgtactcg atcggttgcg cggcgccgtc
     6781 cacccgcggg gccgacgccg gtcccgcgat ggccgcgacc gcaaggtaga gatccggtgc
     6841 gtagcgacga ttgagccgga gttcctcttc gcagcaggtg cgtcgcagtt ccagtgacga
     6901 atagtcgagg aaactcagcc ggaccggctt cttgatcttg tatgcgtacg ggcccgtcag
     6961 cagcacccac gagatgtgcg tctcggcgac gcgaatcggg atttcgaccg cgtgcgggta
     7021 ggcatccggc gacaggagcc gctcgatgag attgccgccg gtgtcgctca tgtctcgatc
     7081 atcgccaagc cgagtctcac gggaactggc ggtcgacgct cgcggcaagc tcgcgcagct
     7141 cggtggagat tcccgccacg acgggcacgg gcccgtcgat cagggcgcaa tcgtcgggaa
     7201 gcgtttcgag ttcccggcgc gcgtgtttcc ggatctcggc gagctcgggc ctcgcgacca
     7261 ccttgccggc gcgcatcgcg ggctgcagca gcgcccggcc gggcagtgtt tcgctggcca
     7321 cgccgacgac gtccgacgca atgcgaccgc gttcgtccag cgtgcggaac acctggcgcg
     7381 ggcccggcca cgtcgccttg ccgggcgagc gcttgcgccg ggcgcgaccg gcgtattcct
     7441 gcagcttgta ggcgcagtcg aggtacggtg cgtcggcgga gacgtcgagg cgcgtcccga
     7501 ctccgaacga atcgaccggg gccccggagg cgagcagtcg cgcaatcgcg aattcgtcga
     7561 ggttgccgct gcagacgatt cgcgtcctgc cgcagccacc ctcgtcgagg atcctgcgga
     7621 cctcgcgcgc caggtcccct aggttgccac tgtccagccg caccgcgtcg acccggacgc
     7681 cggcctgcac catcgcgacc acctggcgcg cggctcgcag cgtgtcgtac gtgtcgatca
     7741 gcagcgtcag cccgcgaggg tgtatacccg cgaaattcct gaacgccgcg gcctcgtcgt
     7801 cgtgggcctg gatgaacgag tgggccatcg tgccggaaat gggtatgccg aaacggcggc
     7861 cggcctccac cgtcgccgtc gcgtcgaatc ctgcgacgta gcaggcacgc gcggccagca
     7921 gggccgcctc ggcaccgtgc gcgcgccgca taccgaagtc caccagcatc cgtggccctg
     7981 cggccagccg gcagcgcgcc gccttgctcg ccaccatgga ctggaaatgg agcaggttga
     8041 tgacgcggct ctcgacgaat tgcgcctcgg gcagcggcgc cgtcaccctc agtatcggct
     8101 cgtcgacgaa gaacacggtg ccttccggca tcgcgtccac gtcgcccgtg aatcgcatcg
     8161 cggcnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnngccatc tcgtcgagcg
     8281 cgcgttgccc gtcgccgggc tgcacgtcca ccgcgcgcac cacgtccgtg aggaccacga
     8341 cctcgaatcc ggtggcccgg gcgtcattcg tcgacgcgcg tacgcagtaa tccgtcgcca
     8401 ggccagccaa gaacacccgc ttgacgcgag cctgccgcag acggtcggca agctccgtcc
     8461 cgtgaaaggc tgaatacgcc tcctcgtcgg ggcgcgtggc cttggagacg acgcgggcgt
     8521 cgcccggaat gcgaagtgcc ggcgagaagc gcgcgccttc ggatccggcg acgcagtgga
     8581 cgggccacgg gccgccccgg tccacgaagg agcagtggtt gggagggtgc cagtcgcgcg
     8641 tcaggaatac cggcaggccg gcggcgcacc accggtccac caggcgattg atcggagcga
     8701 cgatttcgtc gccgcgaccg acaccgagcg cgcccccggg catgaagtcg acctgcacgt
     8761 cgacgacgag caaagcgtca ccgggtcccg gattcctcat ggcgtacaac gctccggccg
     8821 ggccgtgggg tccacggcgc ctttccgtgc ggtcgcgcac gttcgagggc ttacccatgg
     8881 ctttccgcca agtgtaacct tgggacaccg gggtggagtc cctgtgtgca atacttatgg
     8941 ccgcgggcag ccctggttcg acaatgaaca cgaatcaccg ggaaacttga gaagggcaac
     9001 tatcgtgacc attcccgttc aaatcacgtt tcgtcacatg gaaacctcgc aggccgtcga
     9061 gacccgggtg cgcgaactcg cgagccacct cggcgtgttc agcgaccgga tcacgtcgtg
     9121 ccgcgtggtc gtggactcgc cccaccggca ccaccaccag ggcaaggtgt acaacgtcaa
     9181 ggtccagctc gcgctgccgg gcgaggacgt ggtcgtggac atggagcgcc cgatgcgcga
     9241 cggccacgag gacgtctacg tggtgatccg cgacgcgttc gacgcggcca agcgccagct
     9301 gcagcagcgc atgtcctcgc tgcgcaacga ggagaagcga cgcgagacgc tttcgtaacc
     9361 gaggtcccgg gccggaaggc gcgaccggga acgcaggttc cgccgcgccc ggttgggagc
     9421 acttcccgca cggcgcggac atcggcatcc gcgggcgcgg gaccacgatg gccgaagcgt
     9481 tcgcgcaggc cgcccgggcg ttgacgtccg tggtcaccga cctcgccctg gtgcggccgc
     9541 agtcgacggt gcacatccgc tgcagcgcgc ccgatcgcga cctgctcctg tacgagtggc
     9601 tgaatgcgct cgtctacgac atggccacgc ggcacatgct gttctccggc ttcgcggtcc
     9661 acatcgacga cggagtgctc gatgcggacg cgaccggcga gccggtggac atcgcacgcc
     9721 acgcgcccgc cgtggaagtc aaaggcgcga cgatgaccga gctcgccgtg cggcaggacg
     9781 ccgacggtcg ctggctcgcg cagtgcgtgc tcgacgtgtg aggcgcatgc ggggatgaac
     9841 ctcgagggca ccaggcggga ctccgaatac cggtgggtca tcgagccgac cggtccgatg
     9901 cgcgtgcccg cggtcctcta cgcggacgac gagctgatcc gtggcatgga cgacaaggtc
     9961 agggaccagg tctgcaacgt cgcggcgctg cccggcatcg tgggcgcggc ctatgcgatg
    10021 ccggacgccc actggggcta tgggttcccg atcggcggtg tggcggcatt cgacgcggac
    10081 cagggcggcg tcgtgtcggg cggcggcgtc ggcttcgaca tttcctgcgg agtgcgcacg
    10141 ctgctcacgg gcctgcaacg tgacgatgtg ctcgcgtgcc agcgcaagct tgccgacgaa
    10201 ctggcgcgtg ccattccggc gggcgtgggc agcaccgggc gcctgcacct gtcggaagac
    10261 gagatggacg cgatgctgac ggacggcgcg cgctgggcgg ttgcgcgcgg ctacggcagc
    10321 gaagccgacc tggcgcgcat cgaggagcaa gggtgcatct ccggcgcgcg gccggccgcg
    10381 gtgtcgcagc gagcgcgcaa gcgccagcgc gacgagatgg gcacgctggg gtccgggaat
    10441 cattatctcg aggtccagtg ggtcgccgag gtgcacgacg ctgccattgc caccggattc
    10501 ggcctggcgc cgggggacgt ggtggtgagc atccactgcg gctcgcgtgg cctcggtcac
    10561 cagatcggca cggactacct gcgcgacatg gcgatccagg cgccatcgtt cggcatccgc
    10621 ctgcccgatc gcgaacttgc gtgcgcgccg attgcgtcgg agctcggcca gcggtacctg
    10681 ggcgcgatgc gcgcggcgat gaactgcgcg ctcgccaacc gccaggtgct gacccacctg
    10741 tgccgcgagt cgttccgccg catcctgccg cgcgggcgac tcgatatcct gtacgacgtg
    10801 tcgcacaaca tctgcaagga agaggagcac gaggtcgggg gcagcaggcg ccgcctgcac
    10861 gtgcaccgca agggtgcgac gcgtgcgttc gggccgggga gtgccgccat cccggaggcc
    10921 atgcgccggt tcggacagcc ggtgctcatc ggcggctcga tgggaaccgc atcgtacgtg
    10981 ctggccggcg cggcgaccgg cgaggcgcat gcgttcggtt ccgcgtgtca cggcgcgggt
    11041 cgcagcatgt cgcgacacca ggcgacgcgc cagtggaccg gcggccagct ggtgcgggaa
    11101 ctcgccgggc gggggatcct gatccgcagc ccgtccatgc gcggggtcgc ggaggaggcg
    11161 ccgggcgcct acaagagcgt cgagcgcgtg gtcgacgtgg cggcgcgcgc cgggctggcg
    11221 cacaaggtcg cgcgcctcga gccgctcgtg tgcgtgaaag gatgaaatca tgatcgacgc
    11281 actgctgttt gccccatcgg cgtcgcgcgc gttcgcagaa cgcgtcgcct cggcactggg
    11341 aacgtcgctg tccccgatgg aggagcgcga attcgaaggc ggcgagcaca agactcgccc
    11401 gctcgtgagc gtgcggggcc gtcgcgtgtt cgtgctgcag tcgctgtccg gcgacgccgc
    11461 gggcagcgcg aacgaccggc tttgccgcat gctctttttc ttggccgggc tcaaggacag
    11521 cggcgccgcg cacgtcaccg cctgcgttcc atacctggcc tatgcgcgca aggaccggcg
    11581 caccaaggat cgcgacccgg tgacgctgcg ttacgtcgcg cagctcatcg aggccgtcgg
    11641 cacagaccac gtgatcggcc tcgatgccca caacctggcc gctttcgaga gtgcgttccg
    11701 ctgtccggtc gaccacctgg aagcggcgcc gctgctcgcg acctggctgg cgcagcaggc
    11761 cggcgacgct ccggtctgca tcgtctcgcc cgacatcggt ggagccaagc gagcccagcg
    11821 agtgcaggac ctgctcgagt cgcgcactgg ccgcgaggtc ggattcgctc tgatcgaaaa
    11881 gcgacgctcg agcggcgtcg tgagcggcgg cgagcaggtg atcgggccgg tggcggggac
    11941 gcgcgccgtc atcgtggacg acctgatcag cgcgggcacg acgttgatgc gtggcgtgcg
    12001 agcctgccgc gcggccggcg cggtcgccgt cgacgcgttc gcgacgcacg gcgtgttcgt
    12061 gccagcggcg tcgcaactgc tgggcccgga cggtccagac cgcctcatcg tcactgactc
    12121 gatcctgccg acgcgcatcg ggagcgaggc gcgtgcgccg atcgtgctcc cggtgtcgca
    12181 gctctatgcc gaggcgatcc gccgcatctg cagcggcgaa tccgtggtcg cgctgcggga
    12241 gctgcagccg acgcggtgct aggcagcgcc tcccgtcggg tcagtgtgcg gcccggagcg
    12301 ccgccgcgag cgcggtctcg aggtaatcgg ctgcgcgggc gcgccattca tcgggggtgc
    12361 cgccgtccgg ctccgcggat cgccagatgt agagcttggc cttacgggcg gcgcgatggc
    12421 tgcgatacaa gtcgagcagc cgcgccgatg gctcgtcacc gagtttcgcc aggcaggact
    12481 cgagcagccg acggcccgcc gcggcgtgac cgctgcgcgc gcactcgagt tcgagcacgc
    12541 aaagctcctc ggcgcgatcg aggatcctca ggtcgcggtc gaattcgagg cagtcgatga
    12601 tcagcggcgg actcgagagc aggacgtgtt ccggccgcag gtcgccgtgc ccctcgacga
    12661 cgcatccagc gcgtgccctt gcgtccagtt ccgctgcgag cgctgcgatg gccgcgcgtt
    12721 gcagcgtcgc cagtccgcgt gactttgcgg catcgagcga ttcgatcgcc agagcatttg
    12781 cggcaacctg ggccgcgagt cgcgcgccga gctgcccagg accggtgatc gcgggctcgg
    12841 cggccgcgta gaaccccgcc agcagcccgg ccacttcgtc cagcgccgct ggtcgcacgg
    12901 cgccgcgcgc gagcatcgca tcgagaaaca gggttcggtc gaggcgccgc atctcgacca
    12961 gccagtccac ggtctcgccc gggccacctg ccgtgagggt tccgtcggcg cggcgtacca
    13021 gcggcacgat acccaggtag acaccgggcg cgagacggcg attgaggcgc acgtcgtcct
    13081 cggcatcggc gtgtcgcgcc gcgatcgtgc tgtagtccat ggtgtcgcgg cggacgggct
    13141 tgcgcagctt gagggcccgg tcgccgacca ggaagatcca ggcgaagtgc gtttcgatcg
    13201 tcgcgtcgcc ggacccgacc aggcgcttca ggtgcgcgac cttgtcggcg aggctcgccg
    13261 gctcgttcgg cgacgcaggg gatggctgcg tgaggacgtc gctcatcatc gtcgaccggc
    13321 ttcggttcgg ccagcatagc acttaggtac ttgcgcgaat ggcgggtcgg cacccgctgc
    13381 ggaacatgat ccaggcaaac ggagcgattc ggtggaacgc gtattcgagg accgggtgga
    13441 agcgggccgc gcgctggcgc tcgcgctcgg ccggcggcac gacctgcagg gtgccatcgt
    13501 gctcggcctg ccgcgcggcg gcgtgccggt ggccttcgag atcgcgcagg cgctcggcct
    13561 gccgctcgac gtgctcgtcg tgcgcaagct cggcctgccg tggcagccgg agctcgcgat
    13621 gggtgcgatc gcgagcggcg gggccgtcgt gctgaacgaa gacgtggtgc gcctggcggg
    13681 acgcgacgac aaggtgctgg agcaggtgca acagcaggaa ttcgcggagc tcgcgcgccg
    13741 cgagcacgcg ttccggggct cgcggccgcc gctggccgtg aagggccggg cggcgatcgt
    13801 cgtggacgac gggctcgcca ccggcgccac gatggaggcg gccgtgcgtg cactgcgcgc
    13861 cctgggtgcg ctgcgtgtcg tggtcgccgt gccggtggca tcggtcgagg cgcgcgagcg
    13921 catccaggcg gtggccgacg aggttgtgtg cctcgaggca ccgtggcggt tcagcgccgt
    13981 cgggcagtgg taccgcgact tcgcgcagac ctccgacgcc gcggtcaccg agttgctggc
    14041 gcgtgctagt ctcggcacga ctgccgatcc gaggagccgc acatgagcga gctgggtccg
    14101 gaagtgatca aggtcgacga gttcagcgga cgcgaggagg cnnnnnnnnn nnnnnnnnnn
    14161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    14221 nnnnnnnnnn nnnnnnnnnn ngcgagctgg gtccggaagt gatcaaggtc gacgagttca
    14281 gcggacgcga ggaggcgctc ggcctcgacc ccgcgcggct cgtgccgggc agtcccatgc
    14341 ccgaccagca cgtccggaat ctctacacgg acgacacggg ccggttcttc ggcggcatct
    14401 ggcgcagcgg caaaggcgcc tggcacgtct cctacacgga aaacgaattg tgcgtgctga
    14461 ctgaggggcg ggtccgcatc tccgacgacc acgggcgcac gtggaccttt ggtccggggg
    14521 attgcttcgt gatgccagcc ggcttccgcg gtgtgtggga ggtactggag ccggcgcgca
    14581 agttctacgc gatctacgag cctccaccgc ccggctgagc gctccacgag gtgcccgagt
    14641 ccggtgcgcc gggagatcac gccgcaccgg agcggtgcca ctcgcgctca atttcggccg
    14701 gtaatcgcgg cagaatttct ggcatgaatc ctgctttgtg ccctccgtgg gccggcttcc
    14761 gcggagaggg tgcatcctgt atcgcttctg gtacactccg gcgaaaccgg cggcttttcc
    14821 gtggttttat agcaacactt gggggctgac atgaaactga ctcgaggctt ggcgatggtc
    14881 gccttgttgg cgacgggcgc gacggcgcag gctgtcgacc tcagcgcgac gatcaccggc
    14941 gtgacggact acgacttccg cggcatcacg cagtccgccc agaatccggc gctccagggc
    15001 agcgtcgacc tggcgaccga tgccggcttc tacgccggca tctggggcag caacatcgac
    15061 ttcgggccgg gcgatcccaa tgccgaagtg gactggtacg cgggctgggg cggtggcgag
    15121 gacctcacct gggatctcgg catcacctat tacacctaca tcaacggcgg cagtttcaac
    15181 tatcccgagg cccacctggg gttcggctac aagtgggtcg acgtcaaggc gtggtacggc
    15241 tgggactacg gcggcatcga cggcgccgag gagcactact acgagaccaa catcaattac
    15301 ccgctgccgg cgaatttcgg gctggccggc cacatcgggt actcgaatgg cgacggcatc
    15361 aaggccagca cgggccaggg cagctacatg gactggtcgg tcggcgtcac gtacacctgg
    15421 agccacttcg acatgtcgct caagtgggtg gacggcagcg acgccaagta tctcgaccca
    15481 ccgtacctgt acaaggacaa ctgcgactgt ttcaagaaga ccgacgtgtt cagcagcgag
    15541 gcccgcgcga tcttccagat ctccacgacc ttcccgtgga agaaggaagg cgaatagtga
    15601 gcactgcccg ggtctgctga cgcgacctgg atgaacaaac gcccggtctc cgcaaggagg
    15661 ccgggcgttt tctcttgcgg gtggtcgacc ggcgcaggca gccgggcgcg ctacgcggct
    15721 tcctggatcg cctcggccag cttgccgatc agctcgtcga cgtgcttgcg ctcgatgatg
    15781 agcggcggcg acagcgcgat gatgtccccg gtgacgcgcg ccaggagtcc cttctcgaag
    15841 gcgcgcacga atacgtcgaa gccccgcgcg cccggcttgc cggcgatcgg ctcgagttcc
    15901 acggcaccga tcaggccgag attgcgcagg tcgatcacgt gcggcaggcc cttgagggaa
    15961 tgcacggcgt cggcccagta gtcgccgatc tccttcgcgc gcgtgagcag cccctcattg
    16021 cggtagacct gctgggtcgc gagtccggcc gcgcaggcga tcgggtgcgc cgagtaggtg
    16081 tagccgtgga acagctcgat cgcgttctcg ggccccttca tgaatgcgtc gtagatctct
    16141 ttcgtcgccg cgaccgcgcc catcggtatg gagccgttgg tcaggccctt cgccatggtg
    16201 atcatgtccg gcaccacgcc gaagtattcg gccgcgaacg gtgcaccgat gcggccgaac
    16261 ccggtgatca cctcgtcgaa gatgagcagg atcccgtgct tcgtgcagat gtcgcggagc
    16321 ttctgcaggt atcccttcgg cgggatcagc acgcccgtgg acccggcgac cggctcgacg
    16381 atcacggcgg cgatgttgct cgcgtcgtgc agcgcgacga tgcgctcgag ttcgtcggcc
    16441 aggtgcgcgc cccatgacgg ctcgccgcgc gtgtaggcgt tgtgcgccag gtcgtgcgtg
    16501 tgcgccaggt gatccacgcc gggcagttgc gcggcgaaga acttgcggtt gttgaccatg
    16561 ccgccgacgg agatcccgcc gaagccgacg ccgtggtagc cgcgctcgcg accgatcagg
    16621 cgcgtgcggg tgccctcgcc gcggacccgg tggtacgcca gcgcgatctt gagcgccgtg
    16681 tcgacggact ccgagccgga attggtgaag aacacgtggt cgagcccgcg cggcagccac
    16741 ttgaccactt cgttggccag ttcgaaggcg cccgggtgcc ccatctggaa caccggcgcg
    16801 aaatccatct ccgcgatctg gcggctcacc gcctccgtga tttccttgcg accgtgaccc
    16861 gcgttgacgc accacagtcc cgcggcgcca tcgaggacct gccgtccttc gggcgtccag
    16921 tagtacatgc ctgacgcctt cgccagcagc cgcggcttcg ccttgaactg gcggttggcg
    16981 gtgaacggca tccagaatgc ttcgagttcg gcgcgatcca gggggtgctg ggcggacatg
    17041 gggcgtacca gcggcggggc ggccgtatga gggtagacag atgataagaa aaattgacac
    17101 caccctcaaa tcgcgcgaaa acttcgggca cttacgcgct ccctgtgcag gatattgaac
    17161 accccgtggc agacctgacc ctcgacgtgg gcgcccacct gcgcgccgtc cgcactctgt
    17221 acggcctctc gcagcgcgaa ctcgccaagc gtgcgggcgt caccaacggc ctgatctcgc
    17281 tcatcgagca gaaccgcgtc agcccctcgg tgagttcgct caagaaggtg ctcgacggca
    17341 tcccgatgtc catcgcggag ttcttcacgc tggacctgtc ggcgagtccg caggtcttct
    17401 tccgcgccga cgaactcacg gacctcggcg atcgcagcgt ggcgctgcgc cttgtggccg
    17461 cgcggcgcgc aaaccgcgcg atgtcggtga tgcacgagcg ctacgccccg ggcgccgaca
    17521 cgggcgccga catgctgcgt cacgagggcg aggagggcgg agtcgtcgtg cggggccgca
    17581 tcgagctcac ggtgggcggc gagacgcgcc tgctcgggcc cggcgacgcc tactatttca
    17641 cgagcgcgat cccgcatcgc ttccgcaaca gcggcagcga gccttgcgag atcgtctccg
    17701 ccagcacacc gccgaccttc tgaggcctcc catgacgacc aagaccctgg actggcacga
    17761 acgggcgcgc aagctcgccc cggaacaccg tgcattcatc gat
//
LOCUS       DAOMFD010000025        17461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_649_length_17461_cov_32.919855, whole genome shotgun sequence.
ACCESSION   DAOMFD010000025 DAOMFD010000000
VERSION     DAOMFD010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 17461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17461
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_649_length_17461_cov_32.919855"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PL152_02845"
     CDS             <1..969
                     /locus_tag="PL152_02845"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HQR48243.1"
                     /translation="FRQILGFGEYGFPESHAASFALLVYVSAWLKCHEPAAFTAALLN
                     SQPMGFYAPSQLVQDARRHGVEVRPADVRASDWDCTLERCADGTPALRLGLRLVKGLQ
                     EGAARRVVEARDASACVDVQDLAARARLERRELGLLAAAGALEGLAGHRHRAAWNVAG
                     AEASMPLIQRPRIAEGIPLLRAPREGEDIAADYVHTGLTLRRHPLALLRERFDARGIM
                     TAERLRTLPNQATVRTAGLVVTRQRPGSASGVTFVTLEDETGFVNLIVWRDVAERQRR
                     ALVGSRLMGAAGTLQIEGEVMHVIVHRLVDLSRWLGSVVAPSRDFH"
     gene            complement(1012..1260)
                     /locus_tag="PL152_02850"
     CDS             complement(1012..1260)
                     /locus_tag="PL152_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48244.1"
                     /translation="MAEKPESDEFDDEDADDTAGGEDSLDVDRLMQDLDKRKRGAARG
                     LEPAWRKLERLREQKRTSELLSDFDDYDIGDGPSPRKG"
     gene            complement(1361..2140)
                     /gene="trxA"
                     /locus_tag="PL152_02855"
     CDS             complement(1361..2140)
                     /gene="trxA"
                     /locus_tag="PL152_02855"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HQR48245.1"
                     /translation="MTEAPYKFAATSRSFHASVLDASANRPVLVDFWAPWCGPCRTLM
                     PMLDRIADDYGGRFALAKVNTDEEPDVAAQFGIRSIPTVILFSGGRPVEQFVGVQPEQ
                     AIRALLDRYVAGDAAAPDGPAALVARASSLLDARDAEAALATIEQLAELDPDHRSLAA
                     LRTRADLVAAVAAHPDAAALRRAVDADPRDSASRHALAAHHALAGDYATALAEWLELL
                     RLDRRFGDDLARRSLLGAFAILGDGHELVTAYRRKMASLLN"
     gene            2274..3644
                     /locus_tag="PL152_02860"
     CDS             2274..3644
                     /locus_tag="PL152_02860"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HQR48246.1"
                     /translation="MQLSVLDIFKIGIGPSSSHTMGPMNAAGAFVEDLEAKGLLGRTS
                     RVAAQVYGSLALTGHGHCTDRAILLGLEGLRPETLDPADMEPALARIRASGRIRLGGR
                     HEIAFDEPMDLLFHRDQLLPLHPNGMRFTALGADGAVLHRDEFYSTGGGFVVRAADFG
                     ADHEPADAVAVPYPFASARDMLDTARANGLELHDLVLANERAFRSEAATRAALLQVWS
                     VMNACIERGFASTGLLPGVLKVRRRAARLHRVLVESSEARPLDALDWVDAYALAVNEE
                     NAAGGRVVTAPTNGAAGIVPAVIAYYRRFERGASDDGIVRFLLVAGAIGMLYKRNASI
                     SGAEMGCQGEVGVACSMAAGGLVAALNGTNEQVENAAEIGMEHNLGLTCDPIAGLVQI
                     PCIERNAMGAVKAINAARLAMRGDGVQKVSLDQVIATMRQTGQDMSAIYKETSQGGLA
                     VNVPEC"
     gene            3685..4392
                     /locus_tag="PL152_02865"
     CDS             3685..4392
                     /locus_tag="PL152_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR48247.1"
                     /translation="MSKADRVVVITGAFGVLGAAVALAFARRGAHVALLDLAQSPSPA
                     LQRELGQGHLLLGGVDLGEVESSRKAMAAAAMRFGGIDVLVNVAGGFQWQTLLDGAVD
                     TWDQMYLMNLRTAVVSCKVALPALIERGRGRIINVGAGAAAARAGAGMGAYTASKAGV
                     QKLTESLAEELKDRGVTVNAVLPSIIDTPRNRADMPGADFSRWVTPQAIAEVVLFLAS
                     DAAEAITGAAIPVSARA"
     gene            complement(4419..4916)
                     /locus_tag="PL152_02870"
     CDS             complement(4419..4916)
                     /locus_tag="PL152_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQR48248.1"
                     /translation="MKRLTLVRHAKSDTPMPGQQDWDRPLNRRGQRDAPEMARRLRAR
                     KLKPDLILSSPSVRTLATAAIMARELKVDASLVGQDERLYLASPADILAVIRDLGGHA
                     SHVMVFGHNPGMTDCANALSASERIDNMPTCCVFTALFDLDDWSALEFGTGQDAEFDY
                     PKNLG"
     gene            complement(4913..5221)
                     /locus_tag="PL152_02875"
     CDS             complement(4913..5221)
                     /locus_tag="PL152_02875"
                     /inference="COORDINATES: protein motif:HMM:NF025178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme iron oxygenase ferredoxin subunit"
                     /protein_id="HQR48249.1"
                     /translation="MSWVRVAPAASIPPGDHATAEVDGTFVAVFNVGGEFLAVEDVCT
                     HDGGGLAGGPLEGDQVICPRHGARFCLRTGRALSPPAYEPVRCYETRVVEGQVEVRGE
                     "
     gene            complement(5218..5766)
                     /gene="sufT"
                     /locus_tag="PL152_02880"
     CDS             complement(5218..5766)
                     /gene="sufT"
                     /locus_tag="PL152_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative Fe-S cluster assembly protein SufT"
                     /protein_id="HQR48250.1"
                     /translation="MYRADNEPVTFTRDVDAIMVPAGVNVKLREGQQGFITQALGGSF
                     TVYVEGNLFRIAGKDADALGKDAADVPQLPPNASDDDVRELAWQQMKTCYDPEIPINV
                     VDLGLVYSCDVSSEPDGRVVSVTMTLTAPGCGMGDVLVQDVREKVEIIPTVKRADVEL
                     VFDPPWDQSMMSEAARLQTGMM"
     gene            complement(5789..6235)
                     /locus_tag="PL152_02885"
     CDS             complement(5789..6235)
                     /locus_tag="PL152_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR01994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HQR48251.1"
                     /translation="MDLHDLYRDVIVDHNRNPRNFRPMPDADCHADGFNPLCGDRLTL
                     FVRLDGDVIRDVSFQGSGCAISVASASLLTESVKGRTVQDAERLFGTMHAMLTRDDSG
                     VDPATLGKLGALSGVREFPARVKCASLCWHTLDAALHRQAEPVKTE"
     gene            complement(6235..7491)
                     /locus_tag="PL152_02890"
     CDS             complement(6235..7491)
                     /locus_tag="PL152_02890"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HQR48252.1"
                     /translation="MTAAVATHARVRQPLDVEAIRREFPVLARSVHGKPLCYLDNAAS
                     SQRPRRVIDAISTYYETHHANVHRGVHALSQEATDLYEGAREKVRHFIGARSTREVVF
                     VRGTTEAINLVAQSYARPRLRAGDEILISWLEHHANIVPWQMVCEQTGAALRVIPMTR
                     AGELDFEAFRSLLGERTRLLALAHVSNALGTVVPVRRYVAEARRRGVPVLLDGAQAVP
                     HMAVDVQELDCDFYCFSGHKMCGPTGIGVLYGRETLLAAMPPWQGGGDMILSVSFERT
                     TYNDLPWKFEAGTPHIAGAVGLGAAIDYLQQLGLDRIAAAEHDLLAYATARLSSVEGL
                     EIVGTAAEKAAVVSFTMRGVHPHDLGTILDMEGVAIRTGHHCAMPVMDYYGLPATARA
                     SFAFYNTRAEVDRLVAALGVAREMLG"
     gene            complement(7488..8711)
                     /gene="sufD"
                     /locus_tag="PL152_02895"
     CDS             complement(7488..8711)
                     /gene="sufD"
                     /locus_tag="PL152_02895"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HQR48253.1"
                     /translation="MNAAVRADALAPFLAAFESARNEAAASAAVRSAAFEHFAALGLP
                     APRDEAWKYTSLRRLETRRFAPGDKAALSTGVVPAGIRVRTLAAAIADGEPQGALLRV
                     PTGGGTERFAALNAALCADALVVDIAEGAAPDAPLHVRLAASGESSMSHPRLLVRAGR
                     GSTAQVIIHHAGDDEAERFVNSVVDVEASPDSTLHLYRLQDQGLRTFLIERFEATLGQ
                     RATLHVHDAELGASLARLDLHARLAAPGATAELTGLFLADGARHLDAHVRADHLSPGT
                     RSLVDYRGIAAGRGRVVFNGKAVVHPHAQRSEARQTSRNLILTPGAEVDTKPELEIYA
                     DDVKCSHGATTGQLDPAALFYLRSRGLTEAAARGALTRAFAGAVLSRIDAPEFASAVH
                     AILDDRLARLVEIPE"
     gene            complement(8708..9463)
                     /gene="sufC"
                     /locus_tag="PL152_02900"
     CDS             complement(8708..9463)
                     /gene="sufC"
                     /locus_tag="PL152_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012726854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HQR48254.1"
                     /translation="MLKIRDLHARIGDRPILKGIDLEIRAGEVHAIMGPNGSGKSTLA
                     SVLAGREGYEVTRGSVEYLGRDLLAMEPEDRARAGVFLAFQYPVEIPGVNNAYLLKAG
                     LNAVRKQRGEPELDAFEFLALVRDKMRLMQMDESFLNRGVNEGFSGGEKKRNEILQMA
                     VLEPKLALLDETDSGLDIDALRVVANGVNSLRSPERAIVMVTHYQRLLDYIEPDFVHV
                     LSGGKILRSGDRTLALELDKRGYDWIRQEAGAA"
     gene            complement(9485..10933)
                     /gene="sufB"
                     /locus_tag="PL152_02905"
     CDS             complement(9485..10933)
                     /gene="sufB"
                     /locus_tag="PL152_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HQR48255.1"
                     /translation="MSNTPTDLDAIVGQKYRHGFVTEIESDTVPPGLDEDVIRLISRK
                     KGEPQFMLDWRLKAYRHWLTMRRPDWAQLRIAPIDYQAISYYSAPKVRSDGPKSLDEV
                     DPKLLATYEKLGVPLHERARLAGVAVDAVFDSVSVATTFKDRLAKAGVIFCPFSDAVR
                     QHPEIVERYLGSVVPYTDNFFATLNSAVFSDGSFVYVPRGVRCPMELSTYFRINEAKT
                     GQFERTLIVADEGSHVSYLEGCTAPMRDENQLHAAVVELVALADAEIKYSTVQNWYPG
                     DEEGRGGIYNFVTKRGECRGANSRISWTQVETGSAITWKYPSCVLTGDHSVGEFYSVA
                     LANHCQQADTGTKMIHIGRNTRSTIVSKGISAGRGQNTYRGLVKILPSAEGARNHTQC
                     DSLLMGDRCGAHTFPYMEIRNPGAVVEHEATTSKIADDQLFYCRSRGLSEEDAVNMIV
                     NGFCKAVFKELPMEFAVEAQNLMSVSLEGAIG"
     gene            complement(10945..11424)
                     /locus_tag="PL152_02910"
     CDS             complement(10945..11424)
                     /locus_tag="PL152_02910"
                     /inference="COORDINATES: protein motif:HMM:TIGR02944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly regulator"
                     /protein_id="HQR48256.1"
                     /translation="MLRISKLTDYGTVILACLASPPGRRHTAAEVAERTRLALPTVSK
                     LLKSFHRAGIVTSVRGARGGYELARPAQQISAASIIDAIEGPVAITECSGDHSACDLQ
                     TSCSTGNSWQRINAAIRRSLDGISLAQLSGHEAVAIWRPELVGAVRRAPPPRHGGSA"
     gene            11582..12439
                     /locus_tag="PL152_02915"
     CDS             11582..12439
                     /locus_tag="PL152_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR48257.1"
                     /translation="MGSQHGMPSGRGTAAGRDNVAVLRGVHYAVDHKPVFRGLDVAIR
                     RGDITAIMGPSGTGKTTLLRLITGQIRADAGTVEVDGRDVAGLGRRELYALRRRMGML
                     FQNGALLTDLDVFENVAFPLREHARLPERLVRSLVLTKLHAVGLRGAAWLMPQQLSGG
                     MARRVALARAIVMDPEILIYDEPFAGLDPISMGVVMRLIRSLNDALGISSIVVSHDVD
                     EIAKIADQSYILSEGRVVAHGSPRELGEHASPIVRQFMEGLADGPVPFHYPAPDYYRQ
                     LLGAGDGAR"
     gene            12436..13203
                     /gene="mlaE"
                     /locus_tag="PL152_02920"
     CDS             12436..13203
                     /gene="mlaE"
                     /locus_tag="PL152_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013346938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HQR48258.1"
                     /translation="MSLVRELLSGMADFLARAGAMTRFFGRLLLIAPAGLLRPRLVAE
                     QVYNAGALSLAIIMTSGLFVGMVMGLQGHDLLARIGSEESLGTGAALTLLKELGPVVT
                     ALLFAGRAGTALASEIGLMRATDQLSAMEVMAVDPLKRVVLPRFLGGVISMPLLTAIF
                     VTVSIYGVQLIGVQYFGIDRGQFWSEMRSYVEPSDVREGLIKGLVFGVACSFIAVFEG
                     YNAEPTAEGVGRATTRTVVVSSVATLVLDYIVTAILL"
     gene            13216..13743
                     /gene="mlaD"
                     /locus_tag="PL152_02925"
     CDS             13216..13743
                     /gene="mlaD"
                     /locus_tag="PL152_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006392340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015914 - phospholipid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HQR48259.1"
                     /translation="MRQNRAIDVSTGLFVLLGVAAILFLVTQISNREFASQDRAIRVT
                     ARFENVGGLKVGAPVKMAGVTIGRVEEIGYDMKAFKAVVTLGIDARYDEIPKDSDASI
                     FTAGLLGGQYVGISPGGSDEYLKSGDEIEFVQDAIVLENLISKYMFGQGAKDGATQPA
                     APSPSQTPATPAGKE"
     gene            13753..14403
                     /locus_tag="PL152_02930"
     CDS             13753..14403
                     /locus_tag="PL152_02930"
                     /inference="COORDINATES: protein motif:HMM:NF017319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HQR48260.1"
                     /translation="MRIAFVAFCASLMIAGVAHGAAAPATPGPGPQQLIDSVSKELLR
                     EVDANRASLAKDPAKLRALVNRVLLPHFDTEYSARLVLGRYWRDATAQQRQRFIEGFI
                     QSMMRQYGDALLDFTADRLVLLPFRGDPAASTATVRTEVTRDNGTPVPVNYSMHATPD
                     GWKAWDVTIEGVSYVKNFKTDFGAEIEAKGLEAVIQRLETENATPAAPAKPAAKAG"
     gene            14406..14705
                     /locus_tag="PL152_02935"
     CDS             14406..14705
                     /locus_tag="PL152_02935"
                     /inference="COORDINATES: protein motif:HMM:NF024857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HQR48261.1"
                     /translation="MASFTIERVAPGRLSAGGELGFATAAAALELGAAQLAREDEQVV
                     DLGGVTAGDSAGVAVLVEWIATAAASGVSLRYENVPQQMLAIARISDLEDLLIRN"
     gene            complement(14733..15491)
                     /locus_tag="PL152_02940"
     CDS             complement(14733..15491)
                     /locus_tag="PL152_02940"
                     /inference="COORDINATES: protein motif:HMM:NF016236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VacJ family lipoprotein"
                     /protein_id="HQR48262.1"
                     /translation="MLAALGRWVRWIMLACAGVLLAGCATTGERTPGDPFEHGNRDAY
                     RVNETLDRVALKPVAVAYHDHTPSWFQTGVGNFYNNLTYPATAVNDLLQGKLVAASQD
                     TLRFIINTTLGWGGLIDVASGAGLPQHQEDLGQTLGKWGVPAGPFLMVPLLGPSTVRD
                     LPSRVAETVLQPLYWFNFGNARWFSLGLSYVDTRSRLLSVDATLESAYDKYAFVRNAY
                     LQRRQFLVYDGNPPEEPLEEPPLDDTTDEPPPQH"
     gene            complement(15545..17062)
                     /locus_tag="PL152_02945"
     CDS             complement(15545..17062)
                     /locus_tag="PL152_02945"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HQR48263.1"
                     /translation="MSTVRLSRWSCLVVGMLLTPWLGLPTVARASEVQDQLPEMGTAA
                     QQTLSLEDEYRLGRMVMRELRQSGRIVADPETNEYLQTLGLRLSSLANDGQRQFNFFL
                     VNDPTINAFALPGGFVGIHSGLLLETRNESELAGVYAHEISHVTQRHIARSIEAGSRT
                     NLMATAATVAAILLGAIAGVDGNATLGAITAAQGIAQEAQIGFTRENENEADRVGIGL
                     MASAGYDPNAMPAFFEEMGRRTQLSPDQVPELLRTHPVTSARIAESKGRAAQYPPVTP
                     HDSMSYAITRERVRVLTTPAGRDPREYYKSLLVNEPDANVAQLYGYALAQFMAGDAAH
                     AVPTFERLVATHPEVMQFHTALGQALVASGQTAEGLKVLEQARILAPRNVPVTIRYAE
                     ALLQAGRPKRAHEVLLDLFNNVPPTQEQIRLTAIAANAAGDVADAYSYMAEYHLMDGD
                     LPLAANQLELALSVPNLTDVQRARYTARLKEVRDAIPQDRQPPRQDTGQRKSPSG"
     gene            complement(17115..>17461)
                     /locus_tag="PL152_02950"
     CDS             complement(17115..>17461)
                     /locus_tag="PL152_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQR48264.1"
                     /translation="SWGRAATNDLLRNRIKIRSLNFMRGRTFLNRYIILDEAQNLTPK
                     QMKAIVTRAGPGTKLVCLGNIAQIDTPYLSETTSGLTYVVNRFRGWPHYGHVTLLRGE
                     RSRLADVASETL"
BASE COUNT         2540 a         5826 c         6285 g         2810 t
ORIGIN      
        1 ttccggcaga tcctcggctt cggcgagtac ggcttcccgg agtcgcatgc ggcgagtttc
       61 gcgttgctcg tgtacgtgtc ggcgtggctc aagtgccacg agccggcggc gttcactgcc
      121 gcgctgttga acagccagcc gatgggcttc tatgcgccgt cccagctcgt gcaggacgcg
      181 cgtcggcacg gggtcgaggt gcggccggcc gacgtgcggg cgagcgactg ggactgcacg
      241 ctcgagcggt gcgcggacgg aacgccggcc ttgcgcctgg gactgcggct ggtgaagggg
      301 ctgcaggagg gcgcagcgcg gcgcgtcgtc gaggcgcggg acgcgtctgc ctgcgtcgac
      361 gtgcaggatc tcgcggcgcg cgcgcggctg gagcgccgcg agctggggct gctggcggcg
      421 gccggtgcgc tcgaggggct ggcgggtcac cgccatcgcg cggcctggaa cgtggcggga
      481 gcggaggcat ccatgccact gatacaacgg ccgcgcatcg ccgaaggcat cccgttgctg
      541 cgcgcgccgc gcgagggcga ggacatcgcg gccgactacg tgcacaccgg tctcacgctg
      601 cggcggcatc cgctcgcgct gctgcgggag cggttcgacg cgcgcgggat catgacggcc
      661 gagcgcctgc gcacgctgcc gaaccaggcc acggtccgca cggcgggact ggtcgtcacg
      721 cgccagcgcc cgggcagcgc gtcgggcgtc accttcgtga cgctggagga cgagaccggg
      781 ttcgtcaacc tgatcgtgtg gcgggatgtc gccgagcgcc agcgccgggc gctggtcggc
      841 tcacggctga tgggagcggc gggtacgctg cagatcgaag gcgaggtgat gcacgtgatc
      901 gtgcaccggc tggtggactt gtcgcgctgg ctgggcagcg tggtggcgcc ctcgcgtgac
      961 tttcactgac ggcagccgga ccgcgagccc ggcagcagcg gttgtcgctc gtcaaccctt
     1021 gcgcggtgat ggtccgtcgc cgatgtcata gtcgtcgaaa tcgctcagca gttccgaggt
     1081 tcgcttctgc tcgcgcagtc gctcgagctt gcgccaggcc ggctcgagcc cgcgcgccgc
     1141 tccacgcttg cgcttgtcga ggtcctgcat gagccggtcg acgtccagcg agtcttcgcc
     1201 gccggccgtg tcgtcggcat cctcgtcgtc gaactcgtcg gactcgggct tctcggccat
     1261 cacaggaccc gcggtctttc ccccggctgc cgtcggcagt cgatctcgtt gcggctctta
     1321 tacttcgccc gtccgggacc ggcaagaccg gttcccgggc tcagttcagc aggctcgcca
     1381 tcttccggcg gtacgccgtg acgagctcgt gtccgtcgcc gaggatggcg aaggccccga
     1441 gcaggctgcg ccgggcaagg tcgtcgccga accggcggtc aagccgcagc agctcgagcc
     1501 actccgcgag cgccgtcgcg tagtcaccgg ccagcgcgtg atgcgccgca agagcgtggc
     1561 gcgacgcgct gtcgcgaggg tcggcgtcca ccgcccggcg caatgcagca gcgtccggat
     1621 gcgccgcgac cgcggcgacg aggtcggctc tcgtccgcag tgccgcgagc gaccggtggt
     1681 cggggtcgag ttccgcgagc tgctcgatcg tcgccagtgc ggcctcggca tcgcgtgcgt
     1741 cgagcaggga cgaggcacgg gcgaccagcg ccgcagggcc gtccggggcg gcggcgtccc
     1801 cggcgacgta acgatcgagc agcgcacgga tggcctgctc gggctggacg ccgacgaact
     1861 gttcgacggg gcgcccgccg ctgaacagga tcacggtcgg gatgctgcgg atgccgaact
     1921 gcgctgcgac gtccggttcc tcgtccgtgt tgaccttggc gagcgcgaac cggccgccgt
     1981 agtcgtccgc gatgcggtcc agcatcggca tgagcgtgcg gcaagggccg caccacggcg
     2041 cccagaagtc caccagcacc ggccggttcg ccgaagcatc cagtactgac gcatggaagg
     2101 agcggctggt ggcggcaaac ttgtacggtg cttcggtcat cgcggttcct ggccgtgcgc
     2161 agggctcgaa ttgtcgggag acgagcggcc cggggcaagt gcgcgaccgg cccgcggatt
     2221 tgcgctagat tgcgcccggt ccacggccat ccccggatct cgaccccgtc gccatgcagc
     2281 tcagcgtcct cgacatcttc aagatcggca tcgggccgtc gagttcgcac acgatggggc
     2341 cgatgaacgc cgcgggcgcg ttcgtcgagg acctggaggc gaagggcttg ctcggccgca
     2401 cctcgcgagt cgcggcgcag gtctatggct cgctggcgct cacgggtcac ggccactgca
     2461 ccgaccgggc gatcctgctc ggcctggaag gcctgcgacc cgagacgctc gatccggcgg
     2521 acatggaacc ggcgctcgca cggatacggg cctcggggcg catccggctc ggtggtcgtc
     2581 acgagattgc gttcgacgag cccatggacc tgctgttcca ccgcgaccag ctgctgccgc
     2641 tgcacccgaa cgggatgcgc ttcacggccc tcggcgcgga cggcgcagtc ctgcaccggg
     2701 acgagttcta ttccaccggg ggcgggttcg tcgtgcgagc ggcggatttc ggcgccgacc
     2761 acgagccggc cgacgcggtc gcagtgccgt acccgttcgc atccgcacgc gacatgctcg
     2821 acaccgcgcg cgccaacggc ctggaactgc acgacctggt gctcgcgaac gagcgggcgt
     2881 ttcgcagcga ggccgccacg cgcgccgcgc tgttgcaggt ctggtccgtg atgaacgcgt
     2941 gcatcgagcg gggctttgcc agcaccggac tgctgccggg ggtgctgaag gtgcgacggc
     3001 gtgccgcgcg cctgcatcgc gtgctggtgg agtcgagcga agccaggccg ctcgacgcgc
     3061 tggactgggt cgacgcgtat gcgctcgccg tcaacgagga aaacgccgcg ggcgggcgcg
     3121 ttgtgacggc gccgaccaac ggcgcggccg gcatcgtgcc cgcggtcatc gcgtactaca
     3181 ggcgcttcga gcgcggcgcg agcgacgacg ggatcgttcg attcctgctc gtcgcgggtg
     3241 ccatcggcat gctctacaag cgcaacgcgt cgatctcggg tgcggagatg ggctgccagg
     3301 gcgaggtcgg cgtggcctgc tcgatggccg cgggcgggct cgtcgcggcg ctgaacggta
     3361 ccaacgagca ggtcgagaat gcggcggaga tcggcatgga gcacaacctc gggctcacct
     3421 gcgacccgat cgcgggcctg gtgcagatac cctgcatcga gcgcaatgcg atgggtgccg
     3481 tcaaggcgat caatgccgcg cgcctcgcca tgcgcggcga cggcgtgcag aaggtctcgc
     3541 tggaccaggt gatcgccacg atgcgccaga ccgggcagga catgagcgcg atctacaagg
     3601 agaccagcca gggtggactg gcggtgaacg tgccggaatg ctgagcaggg tggttcgtgc
     3661 cggaacctgc acggaggtcc gcagatgagc aaggctgacc gtgtcgtcgt gatcaccgga
     3721 gcgttcggcg tcctgggcgc cgcggtggcg ctggcattcg cccggcgcgg cgcgcacgtg
     3781 gcgctgctcg acctggcgca atcaccttcg ccggcgctgc agcgggaact cggccagggg
     3841 cacctgttgc tcggcggggt cgatctcggc gaggtcgagt cgagccgcaa ggcgatggcc
     3901 gccgccgcga tgcgtttcgg cggcatcgac gtgctggtca acgtcgccgg cgggttccag
     3961 tggcaaacgc tgctcgacgg ggcggtggac acctgggacc agatgtacct catgaacctg
     4021 cgcacggcgg tggtgagctg caaggtcgcg ctgccggcgc tcatcgagcg cggccgtggc
     4081 cggatcatca acgtcggtgc cggcgccgcg gcggcgcggg cgggtgcggg gatgggcgcc
     4141 tacacggcgt cgaaggccgg cgtgcagaag ctgacggaga gtctcgccga ggagctcaag
     4201 gaccgcggcg tgacggtcaa cgcggtgctg ccgtccatca tcgacacgcc gcgcaaccgg
     4261 gcggacatgc cgggcgccga cttcagccgc tgggtgacgc cgcaggccat cgccgaagtc
     4321 gtcctgttcc tcgcgtccga cgccgcggag gcgatcacgg gggcggccat ccccgtctcg
     4381 gcgcgggcgt gaccccggcg gtccgccacg tcgctgggct aaccgaggtt cttcgggtag
     4441 tcgaattcgg cgtcctgtcc ggtgccgaac tccagcgcgc tccagtcgtc gaggtcgaac
     4501 agcgccgtga acacgcagca ggtcggcatg ttgtcgatgc gctcgctggc cgacagggcg
     4561 ttggcgcagt cggtcatgcc cgggttgtgg ccgaagacca tgacgtgaga cgcgtggccg
     4621 ccgagatcgc ggatcaccgc gaggatgtcc gcaggcgacg caagatacag gcgctcgtcc
     4681 tgtcccacga ggctcgcgtc caccttgagt tcgcgcgcca tgatcgcggc ggtcgcgagt
     4741 gtcctcacgg acgggctcga caggatcagg tcgggcttca gcttgcgggc acgcagccgg
     4801 cgcgccatct ccggcgcgtc gcgctgccct cgacgattga gcgggcgatc ccagtcctgc
     4861 tggccgggca tcggcgtgtc ggacttcgca tgtcgcacga gcgtgagtcg cttcattcgc
     4921 cccgcacctc cacctggcct tcgacgacgc gcgtctcgta gcagcgcacc ggctcgtagg
     4981 cgggcggcga cagcgctcgc ccggtccgca ggcagaagcg cgccccgtgc cgcgggcaga
     5041 tcacctggtc gccctcgagc gggccgccgg cgagtccgcc accgtcgtgc gtgcacacgt
     5101 cctcgacggc caggaactcg ccgccgacgt tgaagaccgc gacgaacgtg ccatcgacct
     5161 cggcggtcgc atggtcgccg gggggaatcg acgcggcggg agcgacgcgc acccagctca
     5221 catcatcccc gtctgcagcc gggccgcctc ggacatcatc gactggtccc agggcgggtc
     5281 gaagaccagc tccacgtcgg cgcgcttgac ggtcgggatg atctcgacct tttcgcgcac
     5341 gtcctgcacc agcacgtcgc ccatgccgca gccgggcgcg gtgagcgtca tggtgaccga
     5401 cacgacgcgc ccgtcgggct cggagctcac gtcgcacgag tacacgagcc ccagatcaac
     5461 cacattgatt ggaatttccg ggtcgtagca ggtcttcatc tgctgccacg cgagttcgcg
     5521 cacgtcgtcg tcgctcgcgt tcggcgggag ctgcggcacg tccgcggcat ccttgcccag
     5581 ggcatcggcg tccttgccgg cgatgcggaa caggttgccc tcgacgtaaa cggtgaaact
     5641 tccccccagc gcctgggtaa tgaaaccctg ctggccctcg cgcagcttca cgttgacgcc
     5701 cgcgggcacc atgatcgcgt cgacgtcgcg cgtgaatgtc accggctcgt tgtctgctct
     5761 gtacacgcgg gtgctccgtc ggggtgcgtc actcggtctt gacgggctcg gcctgccggt
     5821 gcagcgcggc gtcgagcgtg tgccagcaga ggctcgcgca cttcacgcgg gccgggaatt
     5881 cacggacgcc ggacagcgcg ccgagcttgc cgagggtggc gggatccacg ccggagtcgt
     5941 cccgggtgag catggcgtgc atggtcccga acagccgctc ggcatcctgc accgtccggc
     6001 ccttgacgct ctcggtcagg agcgatgcgg atgccaccga gatcgcgcag ccgctgccct
     6061 ggaaggagac gtcgcggatg acgtcgccat cgagtcgcac gaacaaggtc aggcggtcgc
     6121 cgcagagggg gttgaagccg tcggcatggc aatccgcgtc cggcatcggg cggaagttcc
     6181 gcggattgcg gttgtggtcg acgatcacgt cgcgatacag gtcgtgcagg tccatcagcc
     6241 gagcatctcc cgcgccacgc cgagggccgc gaccaggcgg tccacctccg cgcgcgtgtt
     6301 gtagaaggcg aaggatgcgc gtgcggtcgc cggcagcccg tagtaatcca tgaccggcat
     6361 cgcgcagtga tggccggtcc tgatcgcgac gccctccatg tcgaggatcg tgccgaggtc
     6421 gtgcggatgg acgccgcgca tcgtgaacga cacgacggcc gccttctccg ccgccgtccc
     6481 cacgatctcc aggccctcga cgctcgacag gcgcgcggtg gcgtacgcga gcaggtcgtg
     6541 ctccgccgcc gcgatgcggt cgaggccgag ttgctggagg taatcgatcg ccgcgccgag
     6601 gccgacggcg cccgcgatgt gaggcgtgcc cgcctcgaac ttccagggca ggtcgttgta
     6661 cgtggtgcgc tcgaagctca ccgacaggat catgtcgccg ccgccctgcc aggggggcat
     6721 ggcggcgagc agggtctccc ggccgtagag cacgccgatg ccggtggggc cgcacatctt
     6781 gtggccggag aagcagtaga agtcgcagtc cagttcctgc acgtcgaccg ccatgtgcgg
     6841 gaccgcctgt gcgccgtcca gcagcaccgg gacaccgcgt cgcctggctt cggcgacgta
     6901 gcgacgcacg gggaccaccg tgccgagcgc attcgacacg tgcgcgaggg cgagcagtcg
     6961 cgtgcgctcg ccgagcagcg agcggaaggc ctcgaaatcc agctccccgg cccgcgtcat
     7021 cgggatgacg cgcaaggccg cacccgtctg ctcgcagacc atctgccacg ggacgatgtt
     7081 ggcgtggtgc tcgagccacg agatcaggat ctcgtcgccg gcgcgcagcc gcggccgggc
     7141 atagctctgt gccacgaggt tgatcgcctc ggtcgtgccg cggacgaaga cgacttcgcg
     7201 cgtggagcgc gcaccgatga agtgccgcac cttttcgcgc gcaccttcgt agaggtcggt
     7261 ggcctcctgg ctcagcgcgt gcacgccgcg gtggacgttg gcgtggtgcg tctcgtagta
     7321 cgtcgagatc gcatcgatca cgcggcgcgg gcgctggctg gaggccgcgt tgtcgaggta
     7381 gcacagcggc ttgccgtgga cgctgcgcgc caggaccggg aactcgcgcc ggatcgcctc
     7441 gacgtccagc ggttgcctga cccgggcgtg ggttgcgacg gcggcggtca ttcggggatc
     7501 tccaccaggc gcgcgagccg gtcgtccagg atggcgtgca cggccgatgc gaattcaggc
     7561 gcgtcgatcc gcgacagcac ggcgccggcg aacgcacgcg tcaacgcacc gcgggccgcc
     7621 gcttctgtga ggccccgcga gcgcaggtag aagagggcgg cgggatcgag ctggccggtc
     7681 gtcgcgccgt ggctgcactt cacgtcgtcc gcgtagatct cgagctcggg cttggtgtcg
     7741 acttccgcgc ccggggtcag gatcaggttg cgactggtct gcctggcttc cgatcgctgc
     7801 gcatgtggat gcacgacggc cttgccgttg aacaccacgc gaccgcgacc cgccgcaatc
     7861 ccgcggtagt cgacgaggct gcgggtgccc ggggagagat gatctgctcg cacgtgggca
     7921 tcgaggtgcc gtgcgccgtc ggcgaggaag aggcccgtca gttccgccgt cgcacccggc
     7981 gccgcaaggc gggcatgcag gtcgagacgt gcgagcgagg cgccgagttc ggcatcgtgc
     8041 acgtgaagtg tcgcgcgctg gccgagcgtc gcctcgaagc gctcgatcag gaaagttcgc
     8101 agtccctggt cctggagtcg gtacaggtgg agtgtggagt cagggctggc ctcgacatcg
     8161 acgaccgaat tgacgaagcg ctccgcctcg tcatccccgg catggtggat gatgacctgc
     8221 gcggtgctgc cgcgcccggc ccgcaccagc aggcgcgggt ggctcatgct cgattcgccg
     8281 gacgcggcga gccgtacgtg caagggagca tcgggggccg cgccctcggc gatgtctacg
     8341 accagcgcat ccgcgcacag tgcggcgttg agtgccgcaa accgctccgt gccgccgccg
     8401 gtcggcacgc gcagcagcgc gccctgcggt tcgccgtcgg cgatggccgc agccagcgtg
     8461 cggacgcgga tcccggcggg aaccacgccg gtcgaaagcg cggccttatc gccaggggca
     8521 aacctgcggg tctccaggcg acgcaagctc gtgtacttcc atgcctcgtc gcgcggggcg
     8581 ggcaaaccca gcgccgcgaa gtgctcgaaa gccgcgctgc gcactgcggc gctcgcagcg
     8641 gcttcgttcc gcgcggattc gaaggccgcg aggaacgggg cgagtgcatc ggcgcgcacg
     8701 gcggcgttca tgcagcgccg gcctcctgcc ggatccagtc gtaacctcgc ttgtcgagtt
     8761 ccagcgcgag cgtccggtcg ccgctgcgca ggatcttccc tccggaaagg acgtgaacaa
     8821 agtctggctc gatgtagtcg agcagccgct ggtagtgcgt gaccatgacg atcgcgcgct
     8881 ccgggctgcg caggctgttc acgccgttgg ccacgacccg cagcgcatcg atgtcgagcc
     8941 cggagtccgt ctcgtcgagc agcgcgagtt tcggctcgag caccgccatc tgcaggatct
     9001 cgttgcgctt cttctcgccg cccgagaagc cttcgttgac cccccggttc aggaagctct
     9061 cgtccatctg catcaggcgc atcttgtcgc gcaccagcgc gaggaactcg aacgcgtcga
     9121 gctccggctc gccgcgctgc ttgcgcacgg cgttcagccc cgctttcagg aggtaggcgt
     9181 tgttgacccc cgggatctcg accggatact ggaatgcgag gaacacgccg gcgcgcgcgc
     9241 ggtcctcggg ctccatcgcg agcaggtccc gccccaggta ctcgacgctg ccgcgcgtga
     9301 cctcgtagcc ttcgcgcccg gcgagcacgc tcgcgagcgt gctcttgccg gagccgttcg
     9361 gacccatgat cgcgtggact tcgcccgcgc ggatctcaag gtcgatgccc ttcaggatcg
     9421 ggcgatcgcc gatgcgggcg tgcaggtcgc gaatcttgag caagatgtct ctccagtggc
     9481 tcggtcagcc gatcgcgcct tcgaggctca ccgacatcag gttctgcgcc tcgaccgcga
     9541 actccatcgg cagttccttg aacacggcct tgcagaagcc gttgacgatc atgttcacgg
     9601 cgtcctcttc cgacaggccg cggctgcggc agtagaacaa ctggtcgtcg gcgatcttcg
     9661 aggtcgtcgc ctcgtgctcg acgaccgcgc ccggattgcg gatctccatg tacgggaacg
     9721 tgtgcgcgcc gcagcgatcg cccatcagca gcgagtcgca ctgcgtgtgg ttgcgcgcgc
     9781 cctcggccga cgggaggatc ttcacgagcc cgcgatacgt gttctggccc cggccggccg
     9841 agatgccctt ggagacgatc gtgctgcggg tattgcgccc gatgtggatc atcttcgtgc
     9901 ccgtgtcggc ctgctggcag tggttggcga gcgccacgga gtagaactcg ccgaccgagt
     9961 ggtcgccggt gagcacgcag ctcgggtact tccaggtgat ggccgacccg gtttccacct
    10021 gcgtccagct gatgcgggag ttcgcgccgc ggcactcgcc gcgcttggtc acgaagttgt
    10081 agatgccgcc acggccttcc tcgtcgcccg ggtaccagtt ctggaccgtc gagtacttga
    10141 tctcggcgtc ggcgagcgcg acgagttcga cgaccgcggc gtgcaactgg ttctcgtcgc
    10201 gcatcggggc cgtgcagccc tcgaggtagc tcacgtggct gccttcgtcg gcgacgatca
    10261 gcgtgcgctc gaactgcccg gtcttcgcct cgttgatgcg gaaataggtc gacagttcca
    10321 tcgggcagcg gacgcccctg gggacgtaca cgaacgaccc gtccgagaac acggccgagt
    10381 tgagcgtcgc gaagaagttg tccgtgtacg gcaccacgct gccgaggtaa cgctcgacga
    10441 tctcggggtg ctgccggacg gcgtcggaga acgggcagaa gatcacgccg gccttggcca
    10501 gccggtcctt gaaggtggtc gcgacggaga cgctgtcgaa cacggcgtcc accgccacgc
    10561 ccgcgagccg cgcgcgctcg tgcaggggca cgccgagctt ctcgtaggtc gccagcagct
    10621 tggggtcgac ctcgtcgagg ctcttcgggc cgtcgctccg gaccttcggc gcggagtaat
    10681 aggagatcgc ctggtagtcg atcggcgcga tcctgagctg cgcccagtcc gggcgccgca
    10741 tggtgagcca gtggcggtac gccttcagcc gccagtcgag catgaattgc ggctcgccct
    10801 tcttgcggga gatgaggcgg atgacgtcct cgtcgagccc gggcggcacc gtgtcggact
    10861 cgatctccgt gacgaagccg tgccggtact tctgtccgac gatcgcgtcg aggtctgtgg
    10921 gcgtgttgga catggcagca gggcctatgc gctaccgccg tggcggggcg gcggggcgcg
    10981 acggacggcg ccgaccagct ccggacgcca gatcgcgacg gcctcgtggc cggacagctg
    11041 ggcgagcgag atgccgtcca gcgaccgccg gatcgcggca ttgatccgct gccaggaatt
    11101 ccccgtgctg caggaggtct gcaggtcgca ggcgctgtgg tcgccgctgc attcggtgat
    11161 cgcgacgggc ccttcgatgg cgtcaatgat ggatgccgcg ctgatctgct gggcgggcct
    11221 cgcgagttca tagccgccgc gcgcccctcg gaccgaggtc acgatccccg cgcgatggaa
    11281 gctcttcagc aacttgctga cggtcggcag cgcgagccgc gtgcgctcgg cgacctccgc
    11341 cgcggtgtgc cggcggccgg ggggggacgc gaggcaggcg aggatgaccg tgccgtagtc
    11401 ggtgagcttg ctgatccgca gcatcggggg tgttccttca attgggacga ttttagtcct
    11461 attggtggat ggaggcaaaa ccgtgacggg ggccctcccg gggctaagcg gctgaacctg
    11521 ctatgattcg cgcggtcaat aggcgtcatc ggcgtccgcc gggcgcagga ttcggaggat
    11581 catgggcagt cagcacggca tgccgagcgg acgcggcacg gctgcgggac gggacaacgt
    11641 cgccgtcctg cgaggcgtcc actacgccgt ggaccacaag cccgtcttcc gcgggctcga
    11701 cgtggccatc cgccgcggag acatcaccgc catcatgggc ccgagcggca ccggcaagac
    11761 gaccttgctc aggctcatca ccgggcagat ccgtgccgac gccggcacgg tcgaggtgga
    11821 cgggcgcgac gtcgccggcc tgggccgccg cgagctgtac gcgctcaggc gccggatggg
    11881 catgctgttc cagaatggcg cgctgctcac cgatctcgac gtcttcgaga acgtcgcgtt
    11941 cccgctgcgc gagcacgcgc gcctgccgga gcgcctggtg cgctcgctgg tgctcaccaa
    12001 gttgcacgcg gtcggcctgc gcggagccgc gtggctcatg ccgcagcaac tctccggagg
    12061 catggcgcgc agggtggcgc tcgcgcgcgc gatcgtgatg gatccggaaa tcctcatcta
    12121 cgacgagccg ttcgccgggc tcgacccgat ttcaatgggc gtggtgatgc gactgatccg
    12181 ctcgctgaac gacgcgctcg gcatctcgag catcgtcgtg tcgcacgacg tcgacgagat
    12241 cgcgaagatc gccgaccaga gctacattct ttcggaaggg cgggtcgtcg cgcacggctc
    12301 gccgcgggaa ctgggcgagc acgcctcgcc gatcgtgcgg cagttcatgg aaggcctcgc
    12361 ggacgggccc gtgccgttcc actacccggc gccggactac taccgccagc tgctcggcgc
    12421 cggagacggt gcgcgatgag cctggtgcgg gaactgctgt ccggcatggc cgacttcctg
    12481 gcacgcgccg gcgccatgac gcgcttcttc ggccgcctgc tgctgatcgc gccggcgggc
    12541 ctgctgcggc cgcggctggt cgccgagcag gtctacaacg cgggcgcgct gtcgctcgcg
    12601 atcatcatga ccagcggcct gttcgtcggc atggtgatgg gcctgcaggg gcacgacctg
    12661 ctggcgcgca tcggctcgga ggagtcgctc ggcaccggcg cggcgctcac gctcctcaag
    12721 gaactcggac cggtggtgac ggcgctgctg ttcgcgggcc gggccggcac tgcgctcgcc
    12781 tcggagatcg gcctgatgcg ggcgaccgac cagctctccg cgatggaggt catggcggtg
    12841 gacccgctga agcgcgtggt cctgccgcgc ttcctcggcg gcgtgatctc gatgccgctg
    12901 ctcacggcga tcttcgtcac cgtgagcatc tacggagtgc agttgatcgg cgtgcagtat
    12961 ttcggcatcg accgtgggca gttctggtcc gagatgcgca gctacgtgga gccgtccgac
    13021 gtgcgcgagg gcctgatcaa gggcctcgtg ttcggcgtgg cgtgcagctt catcgccgtg
    13081 ttcgagggct acaatgccga gccgacggcc gaaggcgtcg gccgcgccac cacccggacc
    13141 gtcgtcgtct cctccgtggc gacgctggtc ctcgattaca tcgtgacggc catccttctc
    13201 tgacccgagg cgaccatgcg gcagaaccgc gccattgacg tatccaccgg actgttcgtg
    13261 ctgctcggcg tggcggcgat actgttcctc gtgacgcaga tctcgaaccg cgagttcgcc
    13321 tcccaggatc gcgccatccg cgtcaccgcc aggttcgaga acgtcggcgg cctgaaggtc
    13381 ggtgcgccgg tcaagatggc aggcgtcacg atcggccgcg tcgaggagat cggctacgac
    13441 atgaaggcct tcaaggccgt cgtgacgctc ggcatcgacg cgcgctacga cgagattccg
    13501 aaggacagcg acgccagcat cttcacggcc ggtctgctcg gcggacagta cgtcggcatc
    13561 tcgccgggag ggtccgacga atacctgaag agcggcgacg agatcgagtt cgtacaggac
    13621 gcgatcgtgc tcgagaacct gatcagcaag tacatgttcg gacagggcgc caaggacggt
    13681 gcgacgcagc cggctgcgcc gtcgccgtcc cagacccccg cgaccccggc cggcaaggag
    13741 tgagacgagt ccatgcgtat cgcgttcgtt gcattctgcg cgtccctgat gatcgccggc
    13801 gtggcccacg gcgccgccgc cccggccacg ccgggccctg gcccgcagca gttgatcgac
    13861 agcgtgtcca aggagctgct gcgcgaggtc gacgccaacc gcgcgtcgct ggccaaggat
    13921 ccggcgaagc tgcgcgcgct cgtgaaccgg gtcctgctgc cgcacttcga caccgagtat
    13981 tccgcccggc tggtgctggg ccgttactgg cgcgatgcca ccgcgcagca gcgccagcgc
    14041 ttcatcgagg gattcatcca gtcgatgatg cggcagtacg gcgacgcgct gctcgacttc
    14101 acggccgacc gcctggtgct cctgccgttc aggggagacc cggccgcatc cactgcgacc
    14161 gtgcgcaccg aggtcacgcg cgacaacggg acgcccgtgc cggtgaatta ctcgatgcac
    14221 gcgacacccg acggctggaa agcctgggac gtcacgatcg agggcgtgtc ctacgtcaag
    14281 aacttcaaga cggatttcgg cgccgagatc gaggcgaagg gtctcgaggc ggtgatccag
    14341 aggctggaga ccgagaacgc gaccccggcc gcgccggcca agcccgccgc gaaggcgggc
    14401 tgaggatggc gtcgttcacc atcgagcggg tagcgcccgg tcgcctcagc gccggcgggg
    14461 aactcgggtt cgcgacggct gcagcagcgc tcgagctggg cgccgcgcag cttgcgcgcg
    14521 aggacgagca ggtcgtcgac ctcggcggtg tgaccgcagg cgacagcgcg ggagtggccg
    14581 tgctggtcga gtggatcgcc accgctgcgg ccagcggtgt cagcctgcgc tacgagaacg
    14641 tgccccagca gatgctcgcc atcgccagga tctccgacct cgaggacctg ctgattcgca
    14701 actgacggtt gccgtcaccg ggcgctcggc ggtcagtgct ggggcggagg ttcgtccgtc
    14761 gtgtcgtcga gaggcggttc ctccagcggt tcctccggcg gattgccgtc gtacacgagg
    14821 aactggcgcc gctgcaggta cgcgttgcgc acgaacgcat acttgtcgta ggcgctctcc
    14881 agggtggcgt cgacggacag caggcgcgag cgcgtgtcga cgtaggacag cccgagcgag
    14941 aaccagcgcg cgttgccgaa gttgaaccag tagagcggct ggagcaccgt ttccgcgacg
    15001 cgggacggca ggtcgcgcac ggtcgacgga ccgaggagcg ggaccatcag gaacggcccg
    15061 gccgggacgc cccacttgcc gagcgtctgc cccaggtctt cctggtgctg cggcagtccg
    15121 gcgcccgagg cgacgtcgat caggccgccc cagccgagtg tcgtattgat gatgaaacgc
    15181 agtgtgtcct ggctggccgc gaccagcttg ccctgaagca ggtcgttcac cgcggtcgcc
    15241 gggtaggtga gattgttgta gaagttgccg acgccggtct ggaaccacga cggcgtgtgg
    15301 tcgtgatacg caaccgccac cggcttgagt gcgacgcggt ccagcgtctc gttgacacgg
    15361 taggcatcgc ggttcccgtg ctcgaaggga tcgcccggcg tgcgttcgcc ggtcgtcgcg
    15421 cacccggcga gcagcacccc tgcgcacgcg agcatgatcc atcgcaccca tcgcccaagc
    15481 gccgcgagca tccgtcgctc cgtgtgtggc cgttcccaaa gtccggagat tctacagggg
    15541 attgctagcc gctcggactc ttgcgctggc cggtgtcctg gcgcggcggc tggcgatcct
    15601 gcgggatggc gtcgcgtact tccttgagcc tcgccgtgta gcgggctcgc tgcacgtcgg
    15661 tgaggttggg cacggacaac gcgagttcga gctggttggc ggcgagcggc aggtccccgt
    15721 ccatgaggtg gtattcggcc atgtaggagt aggcatctgc gacgtcaccg gccgcgttgg
    15781 cggcgatcgc cgtgaggcgg atctgctcct gggtcggcgg cacgttgttg aacaggtcga
    15841 gcaggacctc gtgcgcccgt ttcggccgtc cggcctggag cagggcctcg gcgtagcgga
    15901 tggtcaccgg gacgttgcgc ggcgccagga tgcgcgcctg ctcgaggacc ttgagtccct
    15961 cggccgtctg cccggaagcg accagcgcct gcccgagcgc ggtgtggaac tgcatcacct
    16021 cgggatgggt cgcgacgagg cgctcgaacg tggggacggc gtgcgccgcg tcgcccgcca
    16081 tgaactgtgc cagtgcatag ccatagagct gcgcgacgtt cgcgtcgggt tcgttgacga
    16141 ggagcgactt gtagtactcg cgcgggtcgc ggccggccgg cgtagtcagt acccggaccc
    16201 gctcgcgcgt gatcgcgtag ctcatcgaat cgtgcggcgt gaccggcggg tactgggcgg
    16261 cgcggccctt cgattccgcg atgcgcgcgc tggtgacggg gtgcgtgcgc agcagttccg
    16321 ggacctggtc cggactcaac tgtgtgcgac ggcccatctc ctcgaagaag gccggcatcg
    16381 cattcgggtc gtacccggcc gacgccatca ggccgatgcc gacgcggtcc gcctcgttct
    16441 cgttctcgcg agtgaacccg atctgcgcct cctgcgcgat gccctgtgcc gcggtgattg
    16501 cgccgagggt ggcattgccg tctacgcccg cgatggcgcc gagcaggatc gcggccaccg
    16561 tcgcggcggt ggccatgagg ttggtgcgcg agcccgcctc gatgctgcgc gcgatgtggc
    16621 gctgcgtcac gtgggagatc tcgtgggcat agacgccagc gagttcgctc tcgttgcggg
    16681 tctcgagcag cagtccggaa tggatgccga cgaatccgcc gggcagtgcg aatgcgttga
    16741 tggtcggatc gttgaccagg aagaaattga actggcgctg gccgtcgttg gccaggctcg
    16801 acagccgcag tccgagcgtc tgcaggtatt cgttggtctc cgggtccgcg acgatccgtc
    16861 ccgactgccg cagctcccgc atcaccattc gaccgagccg gtactcgtcc tcgaggctca
    16921 gcgtctgctg cgcggcggtt cccatctccg gcaactggtc ctggacctcc gaggcccggg
    16981 ccactgtcgg caggccgagc cacggcgtca ggagcattcc gacgaccagg caggaccagc
    17041 gggacaggcg cacggtgctc atcaggactt tggacccccg tggtaccgac tggttccgcc
    17101 gcgccccggg gggttcagag cgtctccgac gcgacgtcgg cgagccgcga gcgctcgccg
    17161 cgcaacagcg tcacgtggcc gtagtgcggc cagccccgga accggttcac gacgtaggtc
    17221 aggccggacg tcgtctccga gaggtagggc gtgtcgatct gcgcaatgtt gccgaggcac
    17281 accagcttgg tcccggggcc cgcgcgcgtc acgatcgcct tcatctgctt gggcgtgagg
    17341 ttctgcgcct cgtcgaggat gatgtagcgg ttgaggaacg tacggccgcg catgaagttg
    17401 agcgaacgga tcttgatccg gttgcgcagc aggtcgttgg tcgccgcgcg gccccagctg
    17461 c
//
LOCUS       DAOMFD010000026        16911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_693_length_16911_cov_31.864203, whole genome shotgun sequence.
ACCESSION   DAOMFD010000026 DAOMFD010000000
VERSION     DAOMFD010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16911
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_693_length_16911_cov_31.864203"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(142..669)
                     /locus_tag="PL152_02955"
     CDS             complement(142..669)
                     /locus_tag="PL152_02955"
                     /inference="COORDINATES: protein motif:HMM:NF019095.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HQR48265.1"
                     /translation="MFTTQRIWIPLASAALVLAAMTAGAEELQFKADLTGAAQLPEPT
                     TSKATGECTLVVHDDGRKITYTLSVMGLSNAVTAELHLGGPTANGPAVFKLFPRHGAS
                     AKQGPFTGVLAEGTITSADLTGPMQGSPIADLVDELRGGLAYVNIHTNDGVDGSKPGP
                     GNYRLGEIRGQFGPK"
     gene            complement(812..1855)
                     /locus_tag="PL152_02960"
     CDS             complement(812..1855)
                     /locus_tag="PL152_02960"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR48266.1"
                     /translation="MKTPSLLDVVKLLPSRDLTRAAKVLGLTPDSLLKRVGALESSVG
                     SKLMDTAAKALKPGEAGRLVIDHASRIFSQARDLTRDLEELAGLQSPEIIFGTDMFAA
                     ELPLGVAIGRMATANSRLRVHAILADFEQLAKDVMAGRIEFAIADTTSAERHPTRLAI
                     EPVAEHRVFFYTRQGHPLTSAKEHSLETLLVFPLATSRLPHRIAMHVAKAMPMAKVER
                     ETGDLSPSLMLDSFAVAKAAVLAGDAIGLAPLPAIDAELRSGKLALVPFDAPWLHLSY
                     GLFYPRKRSLSRAAQLFVTQLRQVETDLQAREHRALSRMNPKRRPARKNARTAGTSSE
                     PARRRRTAAARRR"
     gene            1977..3788
                     /locus_tag="PL152_02965"
     CDS             1977..3788
                     /locus_tag="PL152_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQR48267.1"
                     /translation="MTSKLLSRRDLEFLLYEWLDVAALASRPRYADHSRETLDGALDS
                     AEKIATDRFLPYRHKGDTEEPQFDGERVHVLPEVRAAFSAFAEAGLIAAAQDYEYGGM
                     QLPVVVDKACFAYFFAANVAWSGYPLLSAANANLLLSHGSQAQIDAFARPQFAGRYTG
                     TMCLTEPQAGSSLSDVATRAEPDGDSPFGPRYRLTGNKMWISGGEHEVAENIVHLVLA
                     RIPGGPPGVKGISLFIVPRSLVSADGKVGERNDVALAGLNHKMGQRGLVNTLLNFGEG
                     RFRPEGRAGAIGYLVGEPHRGLAYMFHMMNEARIGVGLGAAALGYASYLEALEYARNR
                     PQGRPLASKNPASPQVPIVQHTDVRRMLLAQKAYVEGGLALNLYCARLVDDETTAETT
                     QARDDAALLLEILTPVAKSWPSQWCLAASDLAIQVLGGYGYSRDYPVEQLYRDNRLNP
                     IHEGTHGIQALDLLGRKVTMHGGRAFELLGAAVARCIGAARATGDEALAQYGAALENA
                     VARVAQTTRLLHGAGDPERTLANASVYLEAFGHVVVAWMWLEQAMAAHGKPGTFYDGK
                     RQACRYFFRWELPKTGPQFDLLASLDDTTLAMSDEWF"
     gene            3814..4566
                     /locus_tag="PL152_02970"
     CDS             3814..4566
                     /locus_tag="PL152_02970"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR48268.1"
                     /translation="MARSIFITGASSGIGEALAAEFARRGYDIAIAARRAERLQAQAT
                     RLRELGAARVLPVSLDVTDFASIGRALEQARAEFGRLDVVVANAGVGYSFPAGQGKFD
                     QVKQTIDTDLTGAIATIEHALPMLRAQGGGQVVAITSVAGARGMPFLGSYSAAKAGLH
                     RYVQSLRCEVRHEPIVVTELAPGYIDTDMNRGAPSRPFVIPVEKGAAIMARMIERKVG
                     RRYVPVWPWTLLAPIMKLLPTALLAPKRRVRQ"
     gene            4601..5665
                     /locus_tag="PL152_02975"
     CDS             4601..5665
                     /locus_tag="PL152_02975"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQR48269.1"
                     /translation="MSAVNPGTRPVAPQHAFDTARLAAWLEQEVAPLGGPLEVAQFKG
                     GQSNPTYLLSAGGQRYVLRRKPPGKLLPSAHAVDREYRVIRALHGTDVPVARAFALCE
                     DDSVIGTAFYVMEYVTGRVFWDPKLPDLEPAGRALVHDEINRVIAALHAVDPASVGLA
                     DYGKTGEYIARQVARWSKQYQASETEKIEAMDNLIAWLPANVPAGDETRIVHGDYRID
                     NVIFDPVEPRIRAVIDWELSTLGHPLADFAYHCMVWRIPPGLFRGLAGLDFAALGIPS
                     ERDYVAAYCRRTGRAGIPERDWEYYMAYNMFRIAAIAQGVMARALQGNASSAEALQTG
                     RAARPLAELAWSQVERLKLT"
     gene            5676..6899
                     /locus_tag="PL152_02980"
     CDS             5676..6899
                     /locus_tag="PL152_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48270.1"
                     /translation="MDFAHSAKVQDLQRRVAAFMDQHVYPNEARWHAEVDENRRKGDA
                     WVPTRVVEELKQKARAAGLWNLWLPESEYGAGLTNLEYAPLCEIMGRSHIGPEAFNCS
                     APDTGNMEVLVRYGNEAQKKQWLLPLLDGAIRSGFAMTEPAVASSDATNIEASIVRDG
                     NDYIVNGRKWWTSGAGDPRCKILIFMGKTDPANENRHRQQSMILVPMDAPGVKVLRQV
                     PVFGYDDAPHGHSEVEFRNVRVPATNLLLGEGRGFEIAQGRLGPGRIHHCMRLIGAAE
                     RALEAMCKRSLTRVAFHKRLAEQGVWRERIADARMKIDQARFLVLHAAWKMDVAGNKA
                     AQKEIAMIKVVAPNVACQVIDQAIQLFGGAGVSDDFGLGWLYANSRTLRIADGPDEVH
                     RNHIAKMELGRWFTP"
     gene            6903..7709
                     /locus_tag="PL152_02985"
     CDS             6903..7709
                     /locus_tag="PL152_02985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR48271.1"
                     /translation="MKIAGKVALVTGAGRGIGRALCREFARRGAAGIVASDLDGEAAA
                     IVARELGGLGMQCDVSDAVSVAKLVASAVERFGRVDVLVSNAGLGVKALDLDDALSES
                     DADWQRMWSVHVMAHVHACRAVVPGMLERGSGHVVSVASAAGLLSQVGDAAYSVTKHA
                     AVGLAESLAITYGDRGIDVSVVCPQYVATAMTGLDERTEPGSIPGVITVEQAAAIIAD
                     GMEQGSFLILTHPEVLGFFQKKAADYGRWLAGMRRIRDGLRAGGFLKVKP"
     gene            complement(7631..9064)
                     /locus_tag="PL152_02990"
     CDS             complement(7631..9064)
                     /locus_tag="PL152_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR48272.1"
                     /translation="MARMIPVVDLAFLLVDRAEAPANVGIAMLFEPPPGVSAAQAARD
                     IVRAYRAVGPTAPFDVVPEVPPLGVPHWVSPPSVDMKRHVRRATLPAPGTPEQLDEHI
                     ADLHRERFDRSRPLFEVHLVDGLASGHFAMYIKSHHVTWDGRSAAARIFSSLSTEPGP
                     LLPGFHALPPPPRGASDLAGGLRSAVTHALALKELYARVSARATELRAAQGTTRGNTP
                     FAGPHTRLNQRVAAERSYARFSLDLDEMRCIGRAHGGSINDVLLAVCDEGVQRYLRDC
                     GERPTEPLVAMCPVSTRDPGDVEPGTKASTLFVKLGNPRSSAARRLGEIVASTGRAKQ
                     EFHSMTKEASLDFAVLAFGLWLTSDLFGLGAYTRPVVNFVVSNVGGVEGRRYLGRSRL
                     VGTFPVSMIADPAGLNFTSFSQDGRMDVGLVANRAAVPDAQAIARHCLAAWQQLGKAA
                     PPQGLTLRKPPARKPSRIRRMPASQRP"
     gene            9160..10659
                     /locus_tag="PL152_02995"
     CDS             9160..10659
                     /locus_tag="PL152_02995"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR48273.1"
                     /translation="MRQLRGEDAQFLFAESGHANSNITLVSTYDPSTAPGGRVRFKGL
                     LKYIESRLHLSPIFRQKLLRVPLELDYPYWIEDDRFDLEYHVRHISLPKPGDWRQFCI
                     QASRIHARPLDLSRPLWELYLVEGLDTFLDLPPDSFAILAKIHHAAINVKGGAEITTL
                     LHDTTALPPQAEPAEPWFPEPPPSTSVLLARAAFHNLVQPFMVAAPLTRMVRKIAPVV
                     FGTLRDAWLHPERLPVTRFDSEVSPHRVYETRRFTIEEFKRIRALVPGSTVNDAVLAV
                     CGGALRRYLLSHDELPGPSLVSFAPVSVRSGDKAAPAERAINLFRVPLGTDIEDPVRR
                     LRSIHRHTSSADEIGQAVGAKELTDFTKHAPAATLAMTARLVSGYALGIGEHPALANC
                     TVTNVPGPAIPLYLNGARMTYFSAIMPISDGLGLVFAVTSYDGKVIISPTSCREQMPD
                     PEFFALCIRESFQEYLELAGRLAASRGKTRRKAGAKAKGKASRSVRRAA"
     gene            complement(10685..11503)
                     /locus_tag="PL152_03000"
     CDS             complement(10685..11503)
                     /locus_tag="PL152_03000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR48274.1"
                     /translation="MSATARRGKPPRTLYTLLEGRGFLEMAMLPAWLPLLQGTPRGDG
                     HTVLLVPGFTAGDATLVGLRTFLKSRGYHVETWGFGQNTGFKLKFAHALEQKVRFLHH
                     RSEGKVSLVGWSLGGVYSYFTAHAAPECVRTVISLGSPMRFSVGKLQVPMVVNALYRY
                     FAHPMGAGAHLAHVRAKVLKSPPPVPSTCVYSTTDGVVPADAAQLDDSVGQHENIWVP
                     GSHLGLGFNPAVMWILADRLAQPEGEWRPFDPQGLLGAVYERCARHMRTEMAPA"
     gene            11710..12204
                     /gene="queF"
                     /locus_tag="PL152_03005"
     CDS             11710..12204
                     /gene="queF"
                     /locus_tag="PL152_03005"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR03139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HQR48275.1"
                     /translation="MKRQDRSRRTPARSAGTTLRQLGVATALPASPSAAVLERVPNPH
                     PSASYLVRFVNPEFTSLCPVTGQPDFAHLVIDYVPRRWLVESKSLKLYLASFRNHGAF
                     HEDCTLAVATRLVEFLRPRWLRIGGYWYPRGGMPIDVFWQSGRPPAGLWIPDQGVAPY
                     RGRG"
     gene            12262..13704
                     /locus_tag="PL152_03010"
     CDS             12262..13704
                     /locus_tag="PL152_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR48276.1"
                     /translation="MRLIMRLTAMAWAALVSLPAVAEDTAAASAAKFEAVKSVDRHAA
                     ELTGLSDRIWAYAEIALREYKSSAALADYAEKQGFKVQRGVAGMPTAFVATYGQGHPV
                     IGIMGEYDALPGLSQKAVPEKDPLVPGAAGHGCGHNMFGAASLGAALAIKEQIQKGAL
                     KGTIRFFGTPAEENYGGKIYMARDGLFDDVDVMLAWHPGDDTKSDMTSSQAMVDLAVE
                     FHGKAAHAAADPWNGRSAVDAAEIFLVGLNFMREHIKPSSRMHYTIPSAGDVPNVVPE
                     YAKVWLWLRDWKRTEVESLLERVRKLAEGAALMTETTGTVTVQAGDWEELTLVAGQKL
                     IQDNLVWVGLPAYTDDEQAFARAIQRATGKSELGMDASVKPLEGQDAQGGSTDVGDVS
                     WVVPTITVTVATAPEGVPWHAWPVVATGGMSIGHKGLVRASKVLAATMVDLYERPAAI
                     EEIQAEFKAKKGDVVYKAYVPDGPPPVPKN"
     gene            13770..13866
                     /gene="ffs"
                     /locus_tag="PL152_03015"
     ncRNA           13770..13866
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PL152_03015"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            13911..15566
                     /gene="dnaX"
                     /locus_tag="PL152_03020"
     CDS             13911..15566
                     /gene="dnaX"
                     /locus_tag="PL152_03020"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HQR48277.1"
                     /translation="MSYLVLARKWRPRTFDELVGQDHVRRALVNALDSGRIHHAFLFT
                     GTRGVGKTTIARIFAKSLNCEKGVSSKPCGECGACKDIDAGRFVDLLEVDAASRTKVD
                     DTRELLDNVQYAPARGRYKVYLIDEVHMLSTHSFNALLKTLEEPPPHVKFLLATTDPQ
                     KLPVTVLSRCLQFHLRRLPLQQIQERLTAIAQAEKVEFEPAALRAVARGAEGSMRDAL
                     SLLDQVLAFGGGRVLESEVRALLGTLDRRHVEAMLNALAAKDGAALMACVQQLDERAP
                     DYDQALGELAAAIQRMALLQALPDLRGEDEEQDQALAALAGRFTPEDLQLLYQIAIMA
                     RRDLDFAPDARGGFEMALLRMLAFRPESAGAAPAAPAATTPRSSSAQSRGAGPSPASA
                     ASAPAAGASVASSTDPELARESAQAVATAPVPTGDWGAIVAGLGLQGSVSQLAAHCVL
                     VGRVRDVVKLRLDPAAESYNRSQIVERLAQSLSQHFGQGVRLEISVGQSADGEATPAR
                     QQAQVADERLKAAEQAIEADPAVRAMRETFGATVQPGSVRPLN"
     gene            15593..15919
                     /locus_tag="PL152_03025"
     CDS             15593..15919
                     /locus_tag="PL152_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008844819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HQR48278.1"
                     /translation="MKGGIGNLMKQAQQLQANVQKAQAEIAAMEVTGESGGGMVKVTL
                     NGRHEAKRVQIDPSVPLDDREMLEDLVAAAINDATHRLETASQQKMSGLVGGMNLPPG
                     LKLPFG"
     gene            15933..16532
                     /gene="recR"
                     /locus_tag="PL152_03030"
     CDS             15933..16532
                     /gene="recR"
                     /locus_tag="PL152_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011104642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HQR48279.1"
                     /translation="MLYPPSLSRLIEALRCLPGVGPKSAQRMAFHLLERDRDGAREIA
                     AAVSAAVDTLGHCRRCRMFAEGESCPVCASATRDRSLLCVVESPADVVAIEQSASYRG
                     CYFVLMGHLSPLDGVGPVEIGIDAFERLLAEGEVREVILATNPTVEGEATAHFLAEVV
                     ARSGLRASRIAHGVPVGGELEYVDGGTLAHALAGRQAVK"
     gene            complement(16598..>16911)
                     /locus_tag="PL152_03035"
     CDS             complement(16598..>16911)
                     /locus_tag="PL152_03035"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648591.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HQR48280.1"
                     /translation="LEFFDGMSIGSNDMTQLTLGLDRDSAIMARLFDERDDAVKALLH
                     MAIQACRKAGKYIGICGQGPSDHPDLARWLLDEGIESMSLNPDTVVETWLFLADPQSR
                     R"
BASE COUNT         2672 a         5712 c         5963 g         2564 t
ORIGIN      
        1 tgttcgggtc caactggccg atgatcgcgc cgaaaaaggc gctcgaggac ctcgaacggc
       61 tggaactcga cgagcggacc cggctggcgt tcctcggcgg gaacgccgcg cgcgtgttcg
      121 ggctgtgacg ggccgagcga gttacttcgg tccgaactgg ccgcggatct cgcccaggcg
      181 ataattgccg gggccgggct tcgaaccgtc cacgccgtcg ttggtgtgga tgtttacgta
      241 ggcgaggccg ccgcgcagct cgtcgacgag atcagcgatg ggcgagcctt gcatcgggcc
      301 cgtcaggtcc gcggacgtga tggttccttc cgccagcact ccggtgaacg gaccctgctt
      361 cgctgaggca ccgtgccttg ggaacagctt gaagaccgcc ggaccgttcg cagtgggacc
      421 gccgagatgg agttcggccg tcacggcgtt cgacaggccc atcaccgaga gcgtgtaggt
      481 gatcttcctg ccgtcatcat ggacgaccag ggtgcactcg ccggtggcct tcgaggtggt
      541 cggttccggc aactgcgcag ccccggtcag gtcagccttg aactgcagtt cctccgcgcc
      601 ggccgtcatg gcggccagga ccagtgccgc gcttgcaagc ggaatccaga ttcgttgcgt
      661 cgtgaacatg tccgtcatcc tgttgaagcc ggtgaaacgg gccgcccgac cccggggtcg
      721 cgcggcgatt gcactccaga taggcccggc tcgctcgatc gaaaatacgt agtttcgccc
      781 ccctggctgc gcgatgcgca gcagtcggcc gtcagcggcg ccgtgcggcg gctgtgcgac
      841 gccggcgagc cggctcggag gacgtgcccg cggtcctcgc gttctttcgg gcggggcgac
      901 gcttcgggtt catcctcgac agggcgcggt gctcgcgggc ctgcaggtcg gtctcgacct
      961 ggcgcagctg ggtcacgaac agttgcgcgg cacgcgacag cgagcgcttg cgcgggtaga
     1021 acaggccgta gctcaggtgc agccacgggg cgtcgaacgg caccagcgcc agcttgccgg
     1081 agcgcagctc cgcatcgatc gcgggcagtg gcgccagtcc gatcgcgtcg cccgcgagca
     1141 cggccgcctt ggcgaccgcg aagctgtcga gcatcaggct cggcgagaga tcgccggtct
     1201 cgcgctcgac cttcgccatg ggcatggcct tggcgacgtg catcgcgatg cggtgcggca
     1261 gtcgcgacgt ggcgagcggg aacaccagga gcgtctcgag ggaatgctcc ttggcactgg
     1321 taagcgggtg gccctgtcgc gtgtagaaga acacccggtg ctcggccacc ggttcgatcg
     1381 cgagtcgcgt cgggtggcgc tcggcactcg tcgtgtccgc gatcgcgaac tcgatgcggc
     1441 ccgccatcac gtccttcgcg agttgctcga agtccgcgag gatcgcgtgc acgcgcagcc
     1501 gcgagttggc ggtggccatg cgcccgatgg ccacgccgag cggcagttcc gcagcgaaca
     1561 tgtcggtgcc gaagatgatc tcgggcgact gcaggccggc cagttcctcg aggtcgcgag
     1621 tgaggtcgcg cgcctgggag aagatgcggc tagcgtgatc gatcaccagg cgcccggcct
     1681 cgccgggctt gagcgccttg gcggcggtgt ccatcagctt ggagcccacg ctcgactcga
     1741 gcgcaccgac gcgcttcaac agcgagtccg gtgtcaggcc caggaccttg gccgcgcggg
     1801 tgaggtcccg cgaggggagg agcttgacca cgtccagcag gctgggagtc ttcaccatgc
     1861 cctccaaacg ctagcagatg accacccgga tcgcacgagc agctgggccc gcagcccccg
     1921 gaggtatgga ctctacgatc tctcggagcg ccatgtcagc cggtatgatg cgtaccatga
     1981 cctccaagct gctttcgcgc cgcgacctcg agttcctgct ctacgaatgg ctcgacgtcg
     2041 cggccctggc ctcgcgccca cgctacgccg atcattcccg ggagaccctg gacggcgcgc
     2101 tcgactccgc ggagaagatc gcgaccgacc ggttcctgcc gtaccgccac aagggcgaca
     2161 ccgaggagcc gcagttcgac ggcgagcgcg tgcacgtcct gccggaggtc cgggcggcct
     2221 tctcggcgtt tgccgaggcc gggctgattg cggcggccca ggactacgaa tacggcggca
     2281 tgcagctgcc ggtggtcgtg gacaaggcct gcttcgcgta cttcttcgcc gcgaacgtcg
     2341 cctggtcggg ttacccgctg ctgtcggcgg ccaatgcgaa cctgttgctg tcccacgggt
     2401 cgcaggccca gatcgatgcc tttgcccggc cgcagttcgc cggccgctac accgggacca
     2461 tgtgcctgac ggagccgcag gccggatcgt ccctgtccga cgtcgccacc cgcgccgagc
     2521 cggatggcga ctcgccgttc gggccccgct accggctcac cggcaacaag atgtggattt
     2581 ccgggggcga gcacgaagtc gccgagaaca tcgtgcacct cgtgctggcc aggatcccgg
     2641 gcgggccgcc gggcgtcaag ggcatctcgc tgttcatcgt gccgcgcagc ctcgtctcgg
     2701 ccgacggcaa ggtgggcgag cgcaacgacg tggcactggc ggggctgaac cacaagatgg
     2761 ggcagcgcgg actggtgaac acgctgctca atttcggcga aggacggttt cgtcccgaag
     2821 gccgggccgg ggcgatcggc tacctggtcg gcgagccgca ccgcggcctc gcctacatgt
     2881 tccacatgat gaacgaggcg cgcatcggag tcgggctcgg cgcggccgca ctgggctacg
     2941 cgagctacct cgaggcgctc gaatacgcgc gcaaccggcc gcagggccgg ccgctggcgt
     3001 cgaagaaccc cgcgtcgccc caggtcccga tcgtgcagca caccgacgtg cgccgcatgc
     3061 tgctcgcgca gaaggcctac gtcgaagggg ggctcgcgct caatctctac tgcgcgaggc
     3121 tggtcgacga tgagaccacc gcagaaacca ctcaggcacg cgacgacgcc gcgctgctgc
     3181 tggaaatcct gacaccggtc gcgaagtcct ggccgtcgca gtggtgcctg gccgcgagcg
     3241 acctcgcgat ccaggtcctc ggcggttacg gttactcgcg cgactatccc gtggaacagc
     3301 tctatcgcga caatcgcctg aatccgattc acgaaggcac gcacggcatc caggcgctcg
     3361 acctgctcgg gcgcaaggtc accatgcacg ggggccgcgc cttcgagctc ctgggcgcgg
     3421 cggtcgcccg ctgcatcggt gcggcccgcg cgaccggcga cgaagcgctc gcgcagtacg
     3481 gggcggccct ggagaacgcc gtggcgcgcg tcgcccagac cacgcgcttg ctgcacggtg
     3541 ccggcgaccc ggagcgcacc ctggcgaacg cgagcgtcta cctcgaggct ttcgggcacg
     3601 tggtggttgc gtggatgtgg ctcgagcagg ccatggccgc tcacggcaag ccgggtacgt
     3661 tctacgacgg caagcggcag gcctgccgct acttcttccg ctgggagctg ccgaagaccg
     3721 gcccgcagtt cgacctgctc gcgagcctgg acgacacgac gctcgccatg tcggacgaat
     3781 ggttctgagg cgcggcgggc ggcgagggaa gcgatggcac gatcgatatt catcaccggg
     3841 gcgtcctccg ggatcggcga ggcgctggcg gcggaattcg cgcgccgcgg gtacgacatc
     3901 gccattgccg cgaggcgcgc cgagcggctg caggcacagg cgaccaggct gcgggaactc
     3961 ggcgcggcac gcgtgctgcc ggtctcgctc gacgtgactg acttcgcgag catcggccgc
     4021 gccctcgagc aagcccgcgc tgaattcggg cggctcgacg tcgtcgtcgc gaacgcgggc
     4081 gtcggctact cgtttcccgc gggccagggc aagttcgacc aggtcaagca gacgatcgac
     4141 accgacctca ccggtgcgat cgcgaccatc gagcatgcgc tgccgatgct gcgcgcccag
     4201 ggcggcggcc aggtcgtcgc catcacgtcc gtggcgggcg cgcgaggcat gccgttcctc
     4261 gggtcatact cggcggcgaa ggccgggctg catcggtacg tgcagtcact gcgctgcgag
     4321 gtccggcacg agccgatcgt ggtcaccgaa ctggcgccgg ggtacatcga caccgacatg
     4381 aatcgcggcg cgccgagccg gccattcgtc atcccggtcg aaaagggtgc ggcgatcatg
     4441 gcgcggatga tcgagcgcaa ggtcggacgt cgttacgtgc cggtctggcc gtggacgctt
     4501 ctggcaccga tcatgaagct cctgccgacg gccctgcttg cgccgaagcg aagggtcagg
     4561 cagtgagcat cgcgatggca gccctcgagc gaggcgacgc atgagtgcgg tgaatccggg
     4621 caccaggccg gtcgcgccgc agcacgcatt cgacaccgcg cggctggcgg cgtggctcga
     4681 gcaggaggtc gcgccgctcg gcgggccgct cgaggtcgca cagttcaagg gcggccagtc
     4741 gaacccgacc tacctgctgt cggcgggagg gcagcgctac gtcctgcgcc gcaagccgcc
     4801 cggcaagctg ctgccgtcgg cgcacgccgt cgatcgcgag taccgcgtga tccgggcgct
     4861 ccacggcacg gacgtgccgg tcgcacgcgc ttttgcgctc tgcgaggacg acagcgtcat
     4921 cggcaccgcg ttctacgtga tggagtacgt cacgggccgc gtcttctggg acccgaagct
     4981 gcccgacctc gagcccgccg gccgggccct cgtccacgac gagatcaacc gcgtgatcgc
     5041 ggcgctgcat gccgtcgatc cggcgtccgt gggcctcgcg gactacggca agaccggcga
     5101 gtacatcgcg cggcaggtcg cgcgctggag caagcagtac caggcgtccg agaccgagaa
     5161 gatcgaggcg atggacaacc tgatcgcgtg gctgccggcg aacgtgccgg ccggtgacga
     5221 gacgcgcatc gtccacggcg actaccgcat cgacaacgtg attttcgacc cggtcgagcc
     5281 gcgcatccgc gcggtcatcg actgggaact gtcgacactc ggccacccgc tcgccgactt
     5341 cgcgtaccac tgcatggtgt ggcgcattcc acccggcctg ttccgggggc tggctggcct
     5401 cgatttcgcc gcgctcggca ttcccagcga gcgcgactac gtggcggcct actgccggcg
     5461 caccgggcgc gcgggtatcc ccgagcgcga ctgggagtac tacatggcct acaacatgtt
     5521 ccgcatcgcc gcgatcgcgc agggcgtgat ggcccgcgcg ctgcagggca atgcctcgag
     5581 cgccgaggca ctgcagaccg gtcgtgcggc gcgcccgctc gccgagctcg cctggagcca
     5641 ggtcgagcgc ctgaagctca cttgaggaga agacgatgga ttttgcccat tcagcgaagg
     5701 tgcaggacct gcagcggcgc gtcgccgcat tcatggacca gcacgtctat cccaacgagg
     5761 cgcgctggca cgccgaggtg gacgagaacc ggcgcaaggg cgacgcctgg gtgcccacgc
     5821 gcgtcgtcga ggagctgaag cagaaggcga gggcggccgg cctgtggaac ctgtggctgc
     5881 cggagtccga gtacggcgcc ggcctcacga acctcgagta cgcgccgttg tgcgagatca
     5941 tgggtcgatc ccacatcggg ccggaagcct tcaactgctc ggcgcccgac accggcaaca
     6001 tggaagtgct ggtgcgctac ggcaacgaag cgcagaagaa gcagtggctg ctgccgctgc
     6061 tcgacggcgc gatccgctcc gggttcgcga tgaccgagcc cgcggtcgcg tcgtccgacg
     6121 cgaccaacat cgaggcgagc atcgtccgcg acggcaacga ctacatcgtc aacggccgca
     6181 agtggtggac ctcgggcgcc ggcgacccgc gctgcaagat cctgatcttc atgggcaaga
     6241 ccgacccggc gaacgagaac cgccatcggc agcagtccat gatcctggtg ccgatggatg
     6301 cgccgggcgt gaaagtgctg cggcaggtcc cggtgttcgg ctatgacgat gcgccgcacg
     6361 gccactcgga ggtcgaattc aggaacgtgc gcgtcccggc cacgaacctg ctgctcggcg
     6421 aaggccgcgg cttcgagatc gcgcagggcc ggctcggccc gggccgcatc catcactgca
     6481 tgaggctgat cggcgccgcg gagcgcgcgc tcgaggccat gtgcaagcgc tcgctgaccc
     6541 gggtggcgtt ccacaagcga ctcgcggaac agggcgtgtg gcgtgagcgg atcgccgatg
     6601 cgcgcatgaa gatcgaccag gcgcgcttcc tggtcctgca cgccgcgtgg aagatggacg
     6661 tggcgggcaa caaggccgcg cagaaggaga tcgcgatgat caaggtcgtg gccccgaacg
     6721 tcgcctgcca ggtcatcgac caggcgatcc agctcttcgg cggcgcgggc gtgtccgacg
     6781 acttcggact cggctggctg tatgcgaatt cgcgcaccct gcgcatcgcc gacggcccgg
     6841 acgaggtgca tcgcaaccac atcgccaaga tggagcttgg ccgctggttc acgccctgag
     6901 ccatgaagat cgcgggcaag gtcgccctcg tcacgggcgc aggtcggggc atcgggcggg
     6961 cgctgtgtcg cgagtttgcg cggcgcggtg cggcgggaat cgtcgcgtcg gacctggacg
     7021 gcgaggccgc cgcgatcgtc gcgcgtgaac tcggcggcct cggcatgcag tgcgacgtct
     7081 ccgatgccgt ctccgtcgcg aagctcgtgg cgagcgccgt cgagcgattc ggccgggtcg
     7141 acgtgctggt gtcgaatgcc ggtctcggcg tgaaggccct ggacctcgac gacgcgcttt
     7201 cggagagcga cgcggactgg cagcggatgt ggtcggtgca cgtcatggcg cacgtgcacg
     7261 cgtgccgtgc cgtcgtcccc ggcatgctcg agcgcggctc gggacacgtg gtgagcgtgg
     7321 cgtcggcggc aggattgctg agccaggtcg gcgacgccgc ctacagcgtc accaagcacg
     7381 ccgccgtcgg tctcgccgag tcgctggcga tcacctacgg cgaccgcggc atcgacgtgt
     7441 cggtcgtctg tccgcaatac gtcgccaccg cgatgaccgg tctcgacgaa aggaccgagc
     7501 cgggctcgat acccggcgtg atcacggtgg agcaggccgc cgcgatcatc gccgacggca
     7561 tggagcaagg cagcttcctg atcctcacgc accccgaggt gctgggattc ttccagaaga
     7621 aggcggccga ctacggccgc tggctcgcgg gcatgcgccg gattcgcgac ggcttgcgtg
     7681 ccggcggttt cctcaaggtc aagccctgag gcggcgcggc cttgccgagc tgctgccacg
     7741 cggccaggca gtggcgagcg atcgcctgcg cgtccggtac ggcggcgcgg ttcgcgacca
     7801 ggcctacgtc catccggccg tcctgtgaga acgacgtgaa attgaggccc gcgggatcgg
     7861 cgatcatcga cacgggaaac gtgccgacga ggcgcgatcg cccgagatag cgtcgcccct
     7921 cgacgccgcc cacgttcgag accacgaaat tgaccacggg ccgggtgtag gccccgagcc
     7981 cgaacaggtc cgacgtgagc cacagcccga aggcgagcac ggcgaagtcg agcgacgcct
     8041 ccttcgtcat ggagtggaac tcctgcttcg cgcggccggt gctcgcgacg atctcgccca
     8101 gccgtcgggc cgcactggac cgcgggttgc cgagcttcac gaacagcgtg gacgccttgg
     8161 tgccgggctc gacgtcgccc ggatcacggg tcgagaccgg gcacatcgcc accaggggct
     8221 ccgtcggtcg ttcgccgcag tcgcgcaggt agcgctgcac gccttcgtcg cacacggcca
     8281 gcagcacatc gttgatcgag ccgccgtgcg cgcgcccgat gcatcgcatc tcgtcgagat
     8341 ccagcgagaa ccttgcgtag gatcgctccg cggcgacgcg ctggttcagg cgcgtgtgcg
     8401 gtccggcaaa tggcgtgttg ccgcgtgtcg tgccttgcgc ggcgcgcagc tcggtcgccc
     8461 ttgccgacac ccgcgcgtag agctccttga gcgccagtgc gtgggtcact gccgagcgca
     8521 ggcccccggc gaggtcggac gcgcctcgcg gcggcggggg cagcgcatgg aatcccggca
     8581 gcagcgggcc cggctcggtc gacaggctcg agaagatccg cgcggcggcc gagcgcccgt
     8641 cccaggtcac gtgatgcgac ttgatgtaca tcgcgaagtg gcccgaggcg agtccatcga
     8701 cgaggtggac ctcgaaaagc ggccggctgc ggtcgaatcg ctcgcgatgc aggtcggcga
     8761 tgtgttcgtc cagctgctcc ggcgtcccgg gcgcgggcag cgtcgctcgc cgcacgtggc
     8821 gcttcatgtc cacggacggc ggcgacaccc agtgcgggac gccgagcggc ggaacctccg
     8881 gcacgacgtc aaagggagcg gtggggccca cggctcgata tgcccggacg atgtcacgag
     8941 cggcctgcgc ggcgctcacg cccggcggcg gctcgaacag catcgcgatc ccgacgttcg
     9001 ccggcgcctc cgcgcggtcg accaggagaa aagcgagatc caccaccggg atcatgcgag
     9061 ccatggcagc ccaagtatgc gtcatccgca agggcgcggc gcatccgccc aatgctagac
     9121 tcgggcaggc tgcgacgagc agctgcacgg gatccagcca tgcgacagtt gcgcggagaa
     9181 gacgcccagt tcctgttcgc tgaaagcggg cacgccaact ccaacatcac gctcgtctcg
     9241 acctacgacc cgtcgaccgc accgggtggg cgggtgcggt tcaagggctt gctcaagtac
     9301 atagagagcc gcctgcacct gtcgccgatc ttccgacaga agctgctgcg cgtgccgctc
     9361 gagctcgact atccctactg gatcgaggac gatcgattcg acctcgagta ccacgtgcga
     9421 cacatctcgc tgccgaagcc tggcgactgg cggcagttct gcatccaggc ctcgcgcatc
     9481 cacgcgcgtc cgctcgacct gtcgcggccg ctctgggagc tgtacctcgt cgagggcctc
     9541 gacaccttcc tcgacctgcc gcccgacagc ttcgccatcc tggccaagat ccatcacgcc
     9601 gcgatcaacg tgaagggcgg cgcggagatc accacgctgc tccacgacac gacggcgctt
     9661 ccgccccaag ccgagcccgc ggagccgtgg tttcccgagc cgcctccttc gacgagcgtg
     9721 ctgctggcgc gtgcggcctt tcacaaccta gtgcagccgt tcatggtcgc ggcaccgctc
     9781 acgcggatgg tgcgcaagat cgccccggtg gtgttcggga ccttgcggga cgcctggctg
     9841 caccccgagc ggctgccggt cacgcgattc gactcggagg tctccccgca tcgggtctac
     9901 gagacgcgcc gcttcacgat cgaggaattc aagcggatcc gggcgctggt gccgggttcg
     9961 acggtcaacg acgccgtgct cgccgtgtgc ggtggcgccc tgcgccgcta tctgctctcg
    10021 cacgacgagc tgccaggacc gagcctcgtg tcgttcgcgc ccgtctcggt ccgcagtggc
    10081 gacaaggcgg cgccggctga acgtgccatc aacctgttca gggtgccgct cgggaccgac
    10141 atcgaggatc cggtgcgacg cctgcgctcc atccaccgcc atacctccag cgccgacgag
    10201 atcgggcagg cggtcggggc caaggaactc acggatttca ccaagcacgc gccggcagcc
    10261 acgctggcga tgacggcgcg actggtctcg ggctacgcgc tcggcatcgg ggagcacccg
    10321 gcgctcgcga actgcaccgt gactaacgtg cccgggcctg cgatcccgct gtacctcaac
    10381 ggcgcgcgca tgacgtattt ctcggccatc atgccgatct cggacggcct gggcctggtc
    10441 ttcgcggtga cgagctacga cggcaaggtg atcatttcgc ccacctcgtg ccgcgagcag
    10501 atgccggacc ccgagttctt cgcgctgtgc atccgcgagt cgtttcagga atacctggaa
    10561 ctggccggcc ggctcgccgc ctcgcgaggc aagacgcgtc gcaaggccgg ggccaaggcc
    10621 aaggggaaag cgtcgagatc cgtccgacga gctgcgtgag gggcgggcag gccgccccga
    10681 ccggtcaggc gggtgccatc tccgtacgca tgtgccgggc gcagcgctcg tagacggcgc
    10741 cgagcagtcc ctgcgggtcg aacggtcgcc attcgccctc tggctgggcc agccggtccg
    10801 cgagtatcca catgaccgcg ggattgaacc cgagcccgag gtggctgccg ggcacccaga
    10861 tgttctcgtg ctgcccgacc gagtcgtcga gttgtgccgc atcggccggg acgacgccgt
    10921 cggtcgtcga gtagacgcag gtggacggca ccggaggcgg ggacttgagc actttggcac
    10981 ggacgtgtgc gaggtgtgcg ccggccccca tcgggtgggc gaagtaccgg tacagcgcat
    11041 tgacgaccat cggcacctgt agtttgccca ccgagaagcg catcggactg cccagcgaaa
    11101 tcacggtgcg cacgcactcg ggtgccgcgt gcgccgtaaa gtacgaatag accccgccga
    11161 ggctccagcc tacgaggctc accttgcctt cggaccggtg gtgaaggaac cgcaccttct
    11221 gttcaagggc gtgcgcgaac ttgagcttga atccggtgtt ctggccgaat ccccaggtct
    11281 cgacgtggta gccgcgtgac ttcaggaatg tcctcagccc gaccagggtg gcatcgccgg
    11341 ccgtgaatcc cgggaccaga aggacggtgt gtccgtcacc gcgcggagtc ccctgcagca
    11401 gtggcagcca tgcgggaagc atggccattt cgagaaatcc gcgcccctcg agcagcgtat
    11461 acagcgtgcg gggcggctta ccacggcggg cggtcgcgct catcgtgcct cgacgtttca
    11521 cggccactgc ttgtttgcag cgcagcaagc ttaaccctcc ggcctgtgcc tgggtagtcg
    11581 ttgcctacgc gcagccggca gtgcattgca ccgtgccgcg ccgacaggca aagtggcgtg
    11641 cctgcagtcc cggcccgcaa gccatgtgtg gctgaatcca tataagaatc attgggttgt
    11701 gaccattcga tgaagcgaca agacaggtct cggcgcaccc cggcccggtc ggctggcacg
    11761 acgctgcgcc agctcggcgt ggcgacggcg ctgccggcgt ccccctcggc cgccgtgctc
    11821 gagcgcgtgc cgaacccgca tccatccgcg tcgtacctcg tgcggttcgt gaacccggaa
    11881 ttcacgtcgc tctgcccggt gacgggacaa cccgacttcg cccacctcgt catcgactac
    11941 gtgccgcgcc gctggctggt cgagagcaag tccctgaagc tctacctggc gagcttccgc
    12001 aaccacggcg ccttccacga ggattgcacg ctggccgtgg ccacccgcct ggtcgagttc
    12061 ctgcgtccgc gctggctcag gatcggcggg tactggtacc cgcgtggcgg catgccgatc
    12121 gacgtcttct ggcagtcggg ccgcccgccg gcgggcctgt ggatcccgga ccagggcgtc
    12181 gcgccgtatc gagggcgcgg ctgaaccacc gttcctggac gcggctgaaa ttgcgtttct
    12241 gaacatgggg agtactgcgt gatgcgcttg atcatgcgat tgaccgcgat ggcctgggcg
    12301 gcgctcgtat cgctgccggc agtcgcggag gacaccgcgg cggccagcgc cgcgaagttc
    12361 gaggccgtga agtccgtgga ccggcacgcc gccgagctga ccgggctgtc cgacaggatc
    12421 tgggcgtatg ccgagatcgc gctacgcgag tacaagtcct cggccgcgct cgcggattac
    12481 gccgagaagc agggattcaa ggtgcagcgc ggcgtcgccg gcatgccgac ggcgttcgtg
    12541 gcgacgtacg gccagggcca tcccgtcatc gggatcatgg gcgagtacga cgcgttgccg
    12601 gggttgtcgc agaaggccgt gcccgagaag gacccgctgg tgccgggcgc ggcgggtcac
    12661 ggatgcggcc acaacatgtt cggcgccgcg agcctcggcg cggcgctcgc catcaaggaa
    12721 cagatccaga agggcgcgct gaagggaacc atccgcttct tcggcacgcc ggcggaggag
    12781 aactacggcg gcaagatcta catggcgcgc gacgggctgt tcgacgacgt cgacgtcatg
    12841 ctggcctggc atccgggcga cgacaccaaa tccgacatga cctcgagcca ggcgatggtg
    12901 gacctggccg tggaattcca cggcaaggcc gcgcacgcag cggcggaccc atggaatggc
    12961 cgcagcgccg tcgatgccgc ggagatcttc ctggtcgggc tcaatttcat gcgcgagcac
    13021 atcaagccgt cgtcgcgcat gcactacacg atcccgtccg ccggcgacgt gcccaacgtc
    13081 gttcccgagt atgcgaaggt ctggctgtgg ctgcgcgact ggaagcgcac ggaggtcgag
    13141 tcgctgctcg agcgcgtccg caagctcgcc gagggcgccg cgctgatgac ggagaccacg
    13201 ggcaccgtga ccgtgcaggc gggagactgg gaagaactca cgctcgtggc cgggcagaag
    13261 ctgatccagg acaacctggt ctgggtcggg ctcccggcct acaccgacga cgaacaggca
    13321 ttcgcgcgcg ccatccagcg ggccacgggg aagtccgagc tcggcatgga cgcgtccgtg
    13381 aagccgctcg agggccagga cgcgcagggc ggatccaccg acgtcggcga cgtgagctgg
    13441 gtcgtgccga cgatcaccgt cacggtggcg acggctcccg agggcgtgcc gtggcacgcc
    13501 tggccggtgg tcgccaccgg gggcatgtcc atcgggcaca aggggctcgt gcgcgcgtcc
    13561 aaggttctgg ccgcgacgat ggtcgacctg tacgagcgtc cggcggcgat cgaggagatc
    13621 caggcggaat tcaaggcgaa gaagggcgac gtggtctaca aggcctacgt ccccgacggg
    13681 ccgccgcccg tgccgaagaa ctgacgccag cctgcggact gacgccacct gcccgggctg
    13741 atatagtcgc cgcccttcat ggtggctcgc gtcggatccc tcgcgacggc cagccgcgaa
    13801 ccccgccagg cccggaaggg agcaacggta gcggccacgc cgggtgccgg gggtattctg
    13861 gcgcgggccg cctccagaca cggcgctgcc cggggagtcc gagcctccgc atgagttacc
    13921 tggtcctggc acgcaagtgg cggccgcgca cgttcgacga actggtcggg caggatcacg
    13981 tccgccgcgc gctggtcaat gcgctcgatt ccgggcgcat ccatcatgca ttcctgttca
    14041 cgggaacgcg tggggtcggc aagaccacga tcgcccgcat cttcgccaag tcgctcaact
    14101 gcgagaaagg cgtgtcgtcg aagccctgcg gcgagtgcgg cgcatgcaag gacatcgatg
    14161 ccggacgctt cgtggacctg ctcgaagtgg acgccgcctc gcgcaccaag gtggacgaca
    14221 cgcgcgagct gctcgacaac gtgcagtacg cgccggcccg gggccgctac aaggtgtacc
    14281 tcatcgacga ggtgcacatg ctctcgaccc actcgttcaa cgcactgctc aagacgctcg
    14341 aggagccgcc gccacacgtg aagttcctgc tcgcgaccac cgacccgcag aagctcccgg
    14401 tgacggtgct gtcgcgctgc ctgcagttcc acctgcggcg cctgccgctg cagcagatcc
    14461 aggagcggct gacggcgatc gcgcaggccg agaaggtcga gttcgagccg gcggcgctgc
    14521 gtgccgtggc ccgcggcgcg gagggcagca tgcgtgatgc gctgtcgctg ctcgaccagg
    14581 tcctcgcctt cggcggagga cgcgtgctcg aaagcgaggt gcgggcgctg ctcgggaccc
    14641 tcgaccgccg tcacgtggag gccatgctga atgcgctcgc cgcgaaggac ggcgccgcac
    14701 tgatggcttg cgtgcagcag ctcgacgagc gcgcgccgga ctacgaccag gcgctcggcg
    14761 aactcgcggc ggcgatccag cgcatggcgc tgctgcaggc gctgccggac ctgcgcggcg
    14821 aggacgagga gcaggaccag gcccttgcgg ccctggcggg ccggttcacg cccgaagacc
    14881 tgcaactgct ctaccagatc gcgatcatgg cgcggcggga cctcgatttc gcgccggacg
    14941 cgcgcggcgg attcgagatg gcgctgctgc gcatgctggc cttccgtccc gaatcggccg
    15001 gggccgcacc ggcggcgccg gctgcgacga cgccgcgttc gagctccgcc cagtcgcgcg
    15061 gtgccgggcc gtccccagcg tccgctgctt ccgcgccggc cgcgggcgcc tcggtggcga
    15121 gctcgaccga tccggagctt gcgcgcgagt ctgcgcaagc cgtggccacg gcacccgtgc
    15181 cgacgggcga ctggggagcc atcgtcgcgg gactcgggct gcagggctcg gtgagccagc
    15241 tcgccgcgca ctgcgttctc gtcgggcgcg tccgggacgt ggtcaagctg cggctcgatc
    15301 cagcggcgga gtcttacaat cggtcgcaaa tcgtcgagcg ccttgcccag tcgctgtcac
    15361 agcatttcgg acagggcgtg cgcctcgaga tcagcgtcgg gcaatccgcg gatggcgagg
    15421 cgacgccggc gcggcagcag gcgcaggtgg cggacgaacg gctcaaggcc gcggagcagg
    15481 cgatcgaagc cgatccggcc gtgcgcgcga tgcgggagac attcggcgcg accgtgcagc
    15541 cggggtcggt ccggccattg aactgagcat ccaagcggga gtcatcgaga ccatgaaggg
    15601 cggcatcggc aacctgatga agcaggcgca gcagctgcag gcgaacgtgc agaaggcgca
    15661 ggcagagatc gcggccatgg aagtcaccgg cgagtccggc ggcggcatgg tgaaggtcac
    15721 gctgaacgga cggcacgagg cgaagcgcgt gcagatcgat ccttccgtgc cgctggacga
    15781 tcgcgagatg ctcgaggacc tcgtcgccgc cgcgatcaac gacgccacgc accggctgga
    15841 aacggcgtcc cagcagaaga tgtccggcct ggtgggtggc atgaatctcc cgccggggct
    15901 gaagctgcca ttcggctgag cgcgctccgg cgatgctcta cccgccgagc ctgagccgcc
    15961 tgatcgaggc actgcgctgc ctgccgggcg tcgggccgaa gtccgcgcag cgcatggcat
    16021 tccacctgct cgagcgcgac cgcgatggcg cgcgcgagat cgccgcggcc gtctctgcgg
    16081 cggtggatac gctcggccat tgccgacggt gccggatgtt cgcggaaggc gagtcgtgcc
    16141 cggtgtgcgc gtcggccacc cgggatcgtt cgctgttgtg cgtcgtggaa tccccggcgg
    16201 atgtcgtggc catcgagcag tcggcgagct atcgcggctg ctacttcgtg ctgatggggc
    16261 acctgtcacc gctcgacggc gtggggccgg tcgaaatcgg catcgacgcg ttcgagcgcc
    16321 tgctggccga aggcgaggtg cgcgaggtca tcctcgcgac caatcccacc gtcgagggcg
    16381 aggcgacggc gcacttcctc gcggaagtcg tcgcgcgctc cggcctgcgg gcctcgcgca
    16441 tcgcgcacgg cgtgccggtc ggcggggaac tcgagtacgt ggacggcggc accctcgcgc
    16501 acgcgctcgc gggacgccag gccgtgaaat aatggggaca ttctcctatt tcgcggccgc
    16561 gaaataggag aatgtcccca ttattggaga gtgtctccta acgtcgtgac tgcggatcgg
    16621 cgaggaacag ccaggtctcg acgaccgtgt ccggattgag cgacatgctc tcgatgcctt
    16681 cgtcgagcag ccagcgcgcc aggtcggggt ggtccgacgg cccctgtccg cagatgccga
    16741 tgtactttcc cgccttgcgg caggcctgga tcgccatgtg cagcagggcc ttcaccgcgt
    16801 cgtcgcgctc gtcgaacagg cgggccatga tcgccgagtc gcgatcgagc ccgagagtga
    16861 gttgcgtcat gtcgttcgag ccgatggaca tgccgtcgaa gaactcgagg t
//
LOCUS       DAOMFD010000027        16102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_764_length_16102_cov_35.039197, whole genome shotgun sequence.
ACCESSION   DAOMFD010000027 DAOMFD010000000
VERSION     DAOMFD010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_764_length_16102_cov_35.039197"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1481
                     /locus_tag="PL152_03040"
     CDS             <1..>1481
                     /locus_tag="PL152_03040"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR48281.1"
                     /translation="LWSQQYTRKLLPALEAETSLSTGFKAIGHLHVATNATRREIQRR
                     EMNFARSQSVEKFELSPSEIAALFPPLETGDLVSGIYTPTDGRVNPVDLAMAYAKAAR
                     MQGVRILEGVSVTDLVIRNQRVVGVHTTSGEIGAEVVVLAAGMWSRQLAARAGVSVPL
                     QAAEHYYLLTEAMAGIHGDLPVVEDPEAYAYLREEGGGLLFGLFEPAAATWSPDRIPD
                     DASFLTLPPDWERMTPFLERAFARLPAMRDVGIKTFFCGPESFTPDGGFLMGEAPELD
                     GLYLAAGLNSLGILSSGGMGAIVAELVATGDSSQDITGIAPGRTQTYESTRWFLADRT
                     PATLGYVFSHAGLPNYKHKTARDVRRLALHDRYAARGASFTTLSGWEMPRWFSQDGAM
                     PEVQNRYGRQPWFEYAAIEHRATRERVGLFDKSFMGKFIVQGRDAARVLDRVSANSLP
                     AVGHNVYTQWLNSHGGIVSDLTITRLGEQEFLLVTSDILQRPT"
     assembly_gap    1482..1581
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1582..2615)
                     /locus_tag="PL152_03045"
     CDS             complement(<1582..2615)
                     /locus_tag="PL152_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQR48282.1"
                     /translation="MKAVVCQNAEFRVADLPEPVPAAGQVLVKILRCGICGSDLHMRH
                     HTDHMHQLMSRVGIGDIFPTAQDPVVYGHEFCCEVLDHGPGCHRKLRPGTRIVSQPMV
                     KSDSGIHLTGYDKRFNGGFAERMLLQEIALVPVPNGLSDELASLTEPMAVGLHAVRRG
                     EVTGKDVAIVIGCGPVGLAVICMLKARGVKTIVASDFSPGRRALAQRCGADVVVDPAA
                     SSPYANWQDFGFMGDLPTAMKLVVDTREKLDRLPLPWHTAWRIAEALGAMPPRPVIFE
                     CVGVPGVLQQIIEGAPLLSRIVVAGVCMQVDRIEPALAIQKEVDLRFVFGHSPLEYRD
                     TVHLIAEGKV"
     gene            2806..3588
                     /locus_tag="PL152_03050"
     CDS             2806..3588
                     /locus_tag="PL152_03050"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQR48283.1"
                     /translation="MNRRPKRVVPAHWLIVLLASIATTAVATGTLPLDGTAWVLKSAA
                     GKPVVSGVVATLQFSAGRVSGSDGCNRYSGTYKATADELHVAPDLATTRMACPGIVDE
                     QARAYVAALTGAQRWRVQGERLELLSSDGKVLATLVAQSLPLAGSSWHATGINNGKQA
                     VVSVLAGSTVTLSFGANGEASGSSGCNHYAGRYETRDSGISFGKLAVTAMACADPAVM
                     EQEQNYFRALATASTLRMEGDRLEFRTADGALAVSFVRATGG"
     gene            3617..4510
                     /locus_tag="PL152_03055"
     CDS             3617..4510
                     /locus_tag="PL152_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HQR48284.1"
                     /translation="MGNFEKQMQKVRTAPGFIAALDQSGGSTPKALRLYGIPESAYTG
                     EAQMMDLIHAMRTRIITSKAFNGDRILAAILFEATMDRSILGQPTADYLWEVKNVVPV
                     LKVDKGLEAEANGVQLMKPMPALGSLLERARAKGIFGTKMRSVVKQANAAGIDAILDQ
                     QFAVARQIIAAGLVPIIEPEVDIKCPDKPAAEDLLKAGILKRLDALAEGQLVMLKLTI
                     PDKDDLYVDCIRHPRVLKVVALSGGYTRDDANARLARQHGMVASFSRALTEGLTAQQT
                     DAEFDAALDRSIESIFRASMT"
     assembly_gap    4512..4611
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4612..5936)
                     /locus_tag="PL152_03060"
     CDS             complement(<4612..5936)
                     /locus_tag="PL152_03060"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQR48285.1"
                     /translation="MTLDSPTRAPTPAASRATAAARSRALIGTLACVALLLPGAGHAA
                     AAWLTETGGADMSMAGAGRAAMSFDGAALAANPAAIAGLHGTQATVALLPVKLDFEFR
                     GEGSTPGSAHNEAAANPAGSLFVVHDLGRVSLGLGVYSYAGLGYDYGNDWVGRYSIEH
                     ARLKTSTVAGAAAYHLTDRLDVGATVGAQYADIDAAAGVNNSAAIYGPPADLPDGRVK
                     LHGSEWAPAGSVGLLYRATDSTRVGLAWTAPVSHTVPLDMRASGLHPVLSTLVPRTSA
                     GELEFTLPQQVTLGATTQVTPATLVGLEVGRQDWSAFGHSTMTMMGQPIRILPDGLQD
                     TWNVAAGVRHQLNPSWNISAGMAYDSSPASKSTVPAYFPVAEQWRFSTGVERRFSDGM
                     VVRAALSVIDMGDVRVSQDGVAWPLPGASDLSGTVSGSRAYVFCVASDFA"
     gene            6047..6928
                     /locus_tag="PL152_03065"
     CDS             6047..6928
                     /locus_tag="PL152_03065"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQR48286.1"
                     /translation="MAGPTTSRSLVHRIIGPPEVSCRLGSAPPLVVESYRVLLDSAVV
                     PPLPAPVMLVHTGGKPLSYRSRRSGAAGRSLPGLVTLVPRLVRGEFALGGVGEGALVY
                     FENEKRIPAWVASLAPSVPVTVVDELVSAIVRQIVAAARDGTGDDLYLASLGNALIIQ
                     GHHAFGRPVHAEHPRGSRSGLMIAHTATEYVRANLDGDLSVEAIARRCGVGATHFSNT
                     FRQVTGMTPHRYVRRARIERACELLRTTALGVGEIADMVGFAGQSHFCTAFTQERGVT
                     PTAYRRSCMETERSVVR"
     gene            complement(6952..7884)
                     /gene="dmeF"
                     /locus_tag="PL152_03070"
     CDS             complement(6952..7884)
                     /gene="dmeF"
                     /locus_tag="PL152_03070"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HQR48287.1"
                     /translation="MQSHPEHPHVHRYDLHNPVAERRTRQVMWLTAVMMVIEIIAGTV
                     FGSMALLADGWHMSSHALALGVSAGAYALARRHANDPRFSFGTWKIEVLGGYTSAVFL
                     VGVAAYMGYESVKRLLAPAPIHFDQAIVVAVVGLVVNVVSAWLLSAGTHSHGHDHDHD
                     HHASHAHHHDLNLRSAYVHVIADAATSVLAIGALLGGKYFGATWLDPVMGIAGTILVG
                     RWAIGLLHDTGRVLLDAEMDAPVVAEVREALHALPEHAKLRDLHVWRVGKGKYACIVS
                     VETDGEYAASEIRRQLAVHEELVHVTVEVVPTRG"
     gene            8030..9400
                     /locus_tag="PL152_03075"
     CDS             8030..9400
                     /locus_tag="PL152_03075"
                     /inference="COORDINATES: protein motif:HMM:NF009057.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HQR48288.1"
                     /translation="MSDPIKYLLDESRIPKHWYNIQADLPKPAPAVLHPGTHKPVGPD
                     DLAPLFPMELILQEVTQEREVPIPEPVRDVYRQWRPSPLYRARRLEQALGTPARIYYK
                     YEGVSPAGSHKPNTAVPQAFYNKQAGVKRLMTETGAGQWGSSLAFAGALYDLEVQVFM
                     VRVSYNQKPYRRALMETYGARCVASPSSETESGRAILAQDPNHPGSLGIAISEAVELA
                     AQRDDTKYALGSVLNHVLLHQTVIGQEAIAQFEMADDYPDVIVGCTGGGSNFAGIAFP
                     FIGEQLRGGRKVRVVAVEPAACPSLTRGTYAYDFGDTAHLTPLTKMHTLGSTFTPPGF
                     HAGGLRYHGMAPLVSHVKQLGLIDAVAYQQTGIFAAGVQFARAEGIVPAPEANHAVKG
                     AIDEAMRCREEGVSRAILFNLCGHGHFDMQAYIDYFAGKLEDRDYDPRELAMALAGLP
                     SVAEPA"
     gene            9451..10215
                     /locus_tag="PL152_03080"
     CDS             9451..10215
                     /locus_tag="PL152_03080"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HQR48289.1"
                     /translation="MQMDAVDARQHAASVAPGWALALRRYNDRMMFDLLGGPRPWKLA
                     WVIDAQKCGTFPVLALLMWYYSGRTPAATSPAAWTYLALHGSYGLVWLLKDLAFPDPN
                     WQRRATVASGLYTFAGLVLYWSFGWLIISGTVIPDYPLPRAVWFALCTTLCILGCAIM
                     IAADAQKYFTLRVRRGLIRDGVHRYVRHPNYLGEMLVYGSFALLVWHWWPWLVLGWIW
                     LTLFATNMVMKEASLSRHEGWTDYKRHSWWLVPFVL"
     gene            10362..>11188
                     /locus_tag="PL152_03085"
     CDS             10362..>11188
                     /locus_tag="PL152_03085"
                     /inference="COORDINATES: protein motif:HMM:NF004785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HQR48290.1"
                     /translation="MRRFVSVFALLCAAVTSFAVPFWGARDSSPAGTPPAALKPGEFV
                     WAPDIAPDGPIVVIVSLTEQRAYVYRNGVQVGYTTVSTGKPGHETPTGIFTILQKDKD
                     HRSSKYNDAPMPYQERLTWDGVALHAGGLPGYPESHGCVHLPSRFAEDLFSITHLGMT
                     VVVVNDMTAPQDVVHPAALVPVSASSGEAEIQSRLVVGEKWRWEPEKSPEGPVSMMMS
                     AADQRMIVLRNGIEIGRSRVYISDPDAPLGTHAFIVKAGEGSGESVILKGAAARNWM"
     assembly_gap    11189..11288
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11305..11919)
                     /locus_tag="PL152_03090"
     CDS             complement(11305..11919)
                     /locus_tag="PL152_03090"
                     /inference="COORDINATES: protein motif:HMM:NF021390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MliC family protein"
                     /protein_id="HQR48291.1"
                     /translation="MSSRRSVVCIVGGLLAWALAPAPGVAAADGPSFDCRTVEAGSIA
                     SLICADSGLSALDRQLAQVYADASKKAVDEHPPVLKAEQRGWIKGRDDCWKSDDRRAC
                     VEQEYRLRIAELQARYRLVSFTGPFTFVCDAPAGGEVVVTHFRTDPPTLIAERGDSVS
                     LMFVQPSGSGARYQGRNESFWEHQGVASVTWGYGAPEMRCKLQH"
     gene            12082..13461
                     /locus_tag="PL152_03095"
     CDS             12082..13461
                     /locus_tag="PL152_03095"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR48292.1"
                     /translation="MDIARELRALIGESGVLDAADVARRSAGVWRPDNLRAAALARPT
                     STEQVAAVLRWCNANGVPAVTQGGLTGLVHGADAGPDEVILSLERMRTIENIDPVQRT
                     ASVQAGVTLEVLQDAADAAQLLFPVDFPARGTATIGGMAATNAGGNRVIRYGMTRDQV
                     LGLEVVLADGTIVSSLNSLIKNNTGYDLRQLFIGSEGTLGVITRLVLRLREQPTTENT
                     AFVGLESFDSVAKFLKHMDRSLGGTLSSFEVMWRSFYRLVTTAPAKGRPPIAQDYPFY
                     VLVEGQGADRELDTRRFNAAMESAFESGLVVDAAIAQSAADSASFWALRDDVEQVLHG
                     GLPVIFDISLPISEMEGFATRLEEGLPREIGEDHRLFIFGHLGDGNLHVIVQVQPAAY
                     AAARPKVEALVYRGLEEFRGAVSAEHGIGLEKKPWLRVSRSEAEIAVMRRLKAALDPH
                     NLLNPGKIL"
     gene            complement(13480..14109)
                     /locus_tag="PL152_03100"
     CDS             complement(13480..14109)
                     /locus_tag="PL152_03100"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR48293.1"
                     /translation="MPDEPSGRQAHWQKVYLTKSADSVSWYRPRLDVSLELLGLAGLC
                     TDSRLIDVGGGASTLVDDLLDQGLRRLTVLDVSAEALAIARRRIGPNEREVTWLVSDV
                     LDAALPAGGYDIWHDRAVFHFLTDPADAARYAEQAAAAVRRGGHAIIGGFAPDGPERC
                     SGLPVARRSAQDIAALFVPSFTLVAKRAERHMTPGGSEQSFAYALLQRV"
     gene            complement(14142..14666)
                     /locus_tag="PL152_03105"
     CDS             complement(14142..14666)
                     /locus_tag="PL152_03105"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HQR48294.1"
                     /translation="MRLREVKLMRVLAALAAVTCVPALAEEPAFMSGPWGVAACDAWN
                     QDPVLTTELVKSKWVKNDAGHGYKVLQLYRSDCGDKPTVELRIALKDDKAQCIYGGPV
                     ETQELDGDVDYVMHATTERWHQMGAGEYGPMKAMMFGRLHFSGPMGEAMGNMGPFGGF
                     LKLAGKVPGSATCP"
     gene            complement(14663..>16102)
                     /locus_tag="PL152_03110"
     CDS             complement(14663..>16102)
                     /locus_tag="PL152_03110"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQR48295.1"
                     /translation="YIHDHVMHFYHLHALDWVDVVSALGADPAATSQLAQSLSGYPRS
                     SPGYFADVQKRLKTFVEGGQLGIFANGYWGHPAYKLPPEANLMAVAHYLDALAWQRDV
                     AELHTIFGGKNPHPNFLVGGVPNPIDLDSDSAINAKRLAQVQELIVRMREFVDQVYVP
                     DTLAIAGFYKDWAAQGEGLGNFMCYGDFPSHGINDPKTFLVPRGVILDRDLSTIHEVD
                     LHDPTQIQEFVARAWYDYGGGSQAGLHPYDGETKLDYTGPKPPYQFLDVEKQYSWLKS
                     PRWKGKAVEVGPLARMLMMYATGNAQAKELVDSTLAKLEVPVQALYSTLGRTAARTLE
                     TKIFADAMQGWYDQLIANIKTGDTRTFDDSMWEPSTWPKTARGAGFMEAPRGALGHWI
                     VIEDEKIANYQAVVPSTWNAGPRDAQGQPGAYEAALVGHELHDPAQPIEILRTIHSFD
                     PCIACAVHVLRPRERHAAGLELQTRRPEA"
BASE COUNT         2529 a         5285 c         5451 g         2537 t
ORIGIN      
        1 tgctgtggtc gcagcagtac acgcgcaagc tgctgccggc gctcgaggcc gagacgagcc
       61 tgtcgacggg attcaaggcc atcggccacc tccacgttgc gaccaacgcc acgcgccgcg
      121 aaatccagcg gcgcgagatg aatttcgcgc gttcgcagag tgtcgagaag ttcgagctct
      181 cgccgtccga gatcgcggcg ctgtttccgc cgctcgagac cggcgacctc gtctcgggca
      241 tctacacgcc gacggacggc cgcgtgaatc ccgtggacct cgcgatggcg tatgcgaagg
      301 cggcgcgcat gcagggcgtg cgcatcctcg aaggcgtgtc agtcacggat ctcgtgatcc
      361 gcaaccagcg ggtcgtcggc gtgcacacca cgagtggcga gatcggcgcc gaagtcgtgg
      421 tactcgctgc gggaatgtgg tcgcgacaac tggcggcgcg cgccggggtg tcggtcccgc
      481 tgcaggcggc ggagcactat tacctgttga ccgaggcaat ggctggcatc cacggcgacc
      541 tgccggtcgt cgaggatccg gaggcctacg cctacctgcg cgaggagggc gggggattgc
      601 tgttcggcct tttcgagcca gcggcggcca cctggagtcc ggatcgcatc cccgacgacg
      661 ccagcttcct gacgctcccg cccgactggg agcggatgac gccgttcctg gagcgggcct
      721 tcgcccggct gccggcgatg cgggacgtgg ggatcaagac attcttctgc ggcccggaga
      781 gcttcacgcc ggatggcggc ttcctgatgg gcgaagcgcc ggaactggac ggcttgtacc
      841 tggcggccgg gctcaattcg ctcggcatcc tgtcctcggg cggcatgggc gcgatcgtgg
      901 ccgaactggt cgcgacgggc gattcgtcgc aggacatcac ggggatcgcg ccggggcgca
      961 cgcagaccta cgagtcgaca cgctggttcc tcgccgaccg cacgccggcc acgctcggct
     1021 acgtgttcag ccacgccggc ctgccgaact acaagcacaa gaccgcgcgt gacgtgcggc
     1081 ggctcgcgct gcacgatcgc tacgccgcgc gtggcgcctc gttcacgacc ctgagcgggt
     1141 gggagatgcc ccgctggttc tcgcaggacg gcgccatgcc ggaagtgcaa aatcgttacg
     1201 gtcgacagcc gtggttcgag tacgccgcga tcgagcatcg cgccacgcgc gagcgcgtgg
     1261 gcctgttcga caagtcgttc atgggcaagt tcatcgtgca gggtcgcgac gccgcgcgcg
     1321 tgctggatcg tgtgagcgcc aattccctgc cggccgtcgg gcacaacgtc tacacgcagt
     1381 ggctgaattc ccacggcggg atcgtgtcgg acctgaccat cacgcgactc ggcgagcagg
     1441 aattcctgct ggtgacgagc gacatcctgc aacggccgac cnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1561 nnnnnnnnnn nnnnnnnnnn nttcaccttg ccctcggcga tcaggtgcac ggtgtcgcgg
     1621 tactcgaggg gcgagtggcc gaagacgaag cgcaggtcca cctctttctg gatcgcgagc
     1681 gccggctcga tgcggtccac ctgcatgcag acgccggcga cgacgatgcg cgacagcagc
     1741 ggcgcgcctt cgatgatctg ctgcagcacg ccgggcacgc cgacgcactc gaagatcacc
     1801 gggcgcggcg gcatggcgcc cagtgcctcg gcgatgcgcc acgccgtgtg ccagggcagg
     1861 ggcaggcggt cgagcttctc gcgcgtgtcc acgacgagct tcatcgcggt cggcaggtcg
     1921 cccatgaagc cgaagtcctg ccagttggcg tacggcgagc tcgcggcggg gtccacgacg
     1981 acgtccgcgc cgcaacgctg cgcgagtgcg cgacggcccg gcgagaagtc gctggccacg
     2041 atcgtcttca cgccccgcgc cttgagcatg cagatcacgg cgaggccgac gggaccgcag
     2101 ccgatcacga tcgcgacgtc cttgccggtg acttcgccgc gacgcaccgc gtgcaagccg
     2161 accgccatgg gctcggtgag ggaggcgagc tcgtcggaca ggccgttcgg caccgggacg
     2221 agggcgatct cctgcagcag catccgctcg gcgaacccgc cgttgaatcg cttgtcatat
     2281 ccggtgaggt gtatgcccga gtcggacttg accatcggtt gcgacacgat gcgcgtcccg
     2341 ggtcgcaact tccggtggca gccggggccg tggtccagga cctcgcagca gaactcgtgg
     2401 ccgtacacca ccgggtcctg cgcggtcggg aagatgtcgc cgatgccgac ccggctcatc
     2461 agctggtgca tgtggtcggt gtgatgacgc atgtgcaggt cggagccgca gatgccgcag
     2521 cgcagtatct tcaccaacac ctggcccgcc gctggcaccg gctcgggcag gtcggcgacg
     2581 cgaaactcgg cgttctggca gacgacggct ttcatgacgg tctcctcgga cggcgggcga
     2641 ccacgcggcg acggcttccg ccgacgatga gccgcgccga ccggaccgtc aatcgtcgtt
     2701 tgccgcgccg ttccttcgcc gaccacccgt gcccgtcgcc cgcatcgaca ggatagactc
     2761 tcgaggcagg ctccaggcgc gggaccctcc cgccggaggt acatggtgaa tcgtcgaccc
     2821 aagcgagtcg tgccggcgca ctggttgatc gtgctgctgg catcgatcgc gaccaccgcc
     2881 gtggccaccg ggacgttgcc gctcgacggc acggcctggg tgctgaaatc agcggcgggc
     2941 aagccggtcg tgagtggagt ggtggcgacg ctgcagtttt ccgctggccg cgtgtcgggc
     3001 tcggatggct gcaatcggta ttccggcacg tacaaagcca ctgccgacga gctgcacgtc
     3061 gctcccgacc tggcgacgac gcgcatggcc tgtcccggga tcgtggacga gcaggcgcgc
     3121 gcgtacgtcg cggcgctcac gggcgcgcag cgatggcggg tgcaggggga gcggctggaa
     3181 ttgctgtcgt ccgacggcaa ggtgcttgcc acgctcgtcg cccagtcgct gcccctggca
     3241 ggatcgagct ggcacgcgac cggcatcaac aacggcaagc aggccgtcgt gagcgtcctt
     3301 gcgggttcga cggtgacgct gtcgttcggt gccaacgggg aggccagcgg gtcatctggc
     3361 tgcaaccatt acgcgggacg ctacgagacc cgggactccg gaatctcgtt cggcaagctc
     3421 gcggtcactg ccatggcctg cgcggacccg gcggtgatgg agcaggagca gaactacttc
     3481 agggcgctcg cgaccgcgag tacgctgcgc atggaagggg atcgcctcga gttccggacc
     3541 gccgacggcg cgctcgcggt gagcttcgtg cgcgcgaccg gcggctgacg ggcggcgacc
     3601 aacacaggag acgacgatgg gaaatttcga gaagcagatg cagaaggtac gcaccgcccc
     3661 gggattcatc gcggcgctgg accagagcgg cggcagcacg ccgaaggccc tcaggctcta
     3721 cggcatcccc gagagtgcgt atacgggcga ggcgcagatg atggacctga tccacgccat
     3781 gcgcacccgg atcatcacca gcaaggcctt caatggcgac cggatcctgg cggcgatcct
     3841 gttcgaggcc acgatggacc gcagcatcct cggccagccg accgcggact acctctggga
     3901 ggtcaagaac gtcgtgccgg tcctcaaggt cgacaagggt ctcgaggccg aggcgaacgg
     3961 tgtgcagctg atgaagccga tgccagccct cggctcactg ctcgagcgtg ccagggccaa
     4021 gggcatcttc ggcaccaaga tgcgctcggt cgtgaagcag gcgaacgcgg ccggcatcga
     4081 cgcgatcctc gaccagcagt tcgccgtcgc gcgccagatc attgccgcgg ggctcgtgcc
     4141 gatcatcgag ccagaagttg atattaagtg tccagataaa cctgcggccg aggacctgct
     4201 caaggccggc atcctgaagc ggctcgacgc gctggccgaa ggacagctcg tcatgctcaa
     4261 gctcacgata ccggacaagg acgacctgta cgtggactgc atccggcacc cgcgggtgct
     4321 caaggtcgtg gcgctctccg gcggatacac gcgtgacgac gcgaatgccc gccttgcccg
     4381 ccagcacggc atggtggcga gcttctcgcg ggcgctcacc gagggcctca ccgcgcagca
     4441 gacggacgca gagttcgacg cggcgctcga ccgctccatc gagagcatct tccgcgcgtc
     4501 gatgacctga gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcgaagtcg
     4621 gacgcgacgc agaacacgta ggcccggctg ccgctgacgg tgcccgagag atcgcttgcg
     4681 ccgggcagcg gccacgcgac gccatcctgc gagactcgta cgtcacccat gtcgatgacc
     4741 gagagtgcgg cgcgcacgac catgccgtcg ctgaagcggc gctcgacgcc cgtcgagaat
     4801 cgccactgct cggccaccgg aaagtaggcc gggacggtcg acttcgacgc cggcgaactg
     4861 tcgtatgcca ttccggcgga tatgttccag gacggattga gctgatgccg gactccggcg
     4921 gcgacgttcc acgtgtcctg cagtccatcg ggaagaattc gaatcggctg gcccatcatc
     4981 gtcatcgtgg agtgcccgaa ggccgaccag tcctggcggc cgacctcgag tccgacgagc
     5041 gtggccggcg tcacctgcgt ggtcgcgccg agcgtgacct gctgcggcaa cgtgaactcg
     5101 agttcgccgg ccgacgtccg cggcaccagc gtggacagga cgggatgcag gccgctcgcg
     5161 cgcatgtcga gcggcacggt gtgcgacacg ggagcggtcc aggcgagccc gacgcgggtg
     5221 ctgtcggtcg cgcgatagag caggcccacg ctgcctgcgg gcgcccactc actgccgtga
     5281 agcttgaccc gcccgtccgg caggtcggcc ggtggaccgt agatcgcggc tgaattgttg
     5341 actcccgctg cggcgtcgat gtcggcgtac tgggccccga ccgtggcgcc gacgtcgagc
     5401 cggtccgtca ggtggtaggc cgccgcgccg gccacggtcg atgtcttcag acgtgcatgt
     5461 tcgatcgagt agcgaccgac ccagtcgttg ccgtagtcgt aaccgagccc cgcatagctg
     5521 tagacgccga gtcccaggga gacgcgcccg aggtcgtgca ccacgaacag gctgccagct
     5581 ggatttgccg ccgcttcgtt gtgcgccgag cctggtgtgc tgccttcgcc gcggaattcg
     5641 aagtcgagct tgacgggcag cagggcgact gtcgcctgcg tgccgtgcag gccggcgatg
     5701 gcggcgggat tggcggccag cgcagcgccg tcgaaggaca ttgcggcgcg ccctgcgcct
     5761 gccatgctca tgtcggcgcc gccggtttcg gtcagccagg cggctgcggc gtgaccggcc
     5821 ccgggaagca gcagcgcgac acaggccagc gtcccgatga gtgcccgcga gcgcgccgct
     5881 gcggttgcgc gcgaggccgc gggtgtgggt gcgcgagtgg gtgaatcgag cgtcatggcg
     5941 atcatccgtg gtcctgatgc cgcagcaggt tacgtcgatc cgcctcgcca ctttgcgcac
     6001 gaccgaagcg cgattttcgt tttcctgcag tacactcccg gtaaccatgg caggacccac
     6061 cacgtccaga tcgctggtcc accgcatcat cggcccgccg gaggtctcgt gccggctggg
     6121 gtcggcgccg ccgctggtcg tcgagtccta tcgtgtcctg ctcgattccg cggtcgtccc
     6181 gcctctgccc gcgccggtga tgctcgtgca cacggggggc aagccgctgt cgtaccggtc
     6241 gcggcgatcc ggtgcggccg gtcgctcgct gcccggcctc gtcacgctgg tgccacgcct
     6301 ggtgcgcggc gagttcgcgc tgggcggggt cggcgaaggc gccctggtgt atttcgaaaa
     6361 tgagaagcgc attcccgcgt gggtcgcatc gctggcgccg tccgtgccgg tgaccgtcgt
     6421 cgacgagctg gtctcggcca tcgtccgcca gatcgtggcc gccgcgcggg acggcaccgg
     6481 cgacgatctc tacctcgcgt cactgggtaa cgcgctgatc atccagggcc atcacgcatt
     6541 cggtcggccg gtgcacgcgg agcacccgcg cgggtcgcgc agcgggctca tgatcgcgca
     6601 tacggccacc gagtacgtcc gcgcgaacct cgatggcgat ctctcggtcg aggccatcgc
     6661 gcgccgctgc ggcgtgggcg cgacgcattt ctcgaacacg ttccggcagg tcacgggcat
     6721 gacgccgcat cgctacgtgc gccgcgcgcg catcgaacgg gcgtgcgagt tgctgagaac
     6781 gaccgcgctc ggcgtcggcg agatcgccga catggtcggg tttgccgggc agagccactt
     6841 ctgcacggcc ttcacgcagg agcgtggggt gacaccgacc gcctatcgtc gcagctgcat
     6901 ggaaacggag cgatctgttg tgcgctgacg cacgggtcgc gcgccgccgt ttcagccccg
     6961 ggtcgggacg acctcgaccg tgacgtgcac gagttcctcg tgcaccgcga gttggcggcg
     7021 tatctcggac gccgcgtact cgccatccgt ctcgacgctc acgatgcagg cgtacttgcc
     7081 cttgccgacg cgccacacgt gcaggtcgcg cagcttcgcg tgctccggca gcgcatgcag
     7141 cgcttcgcgc acctcggcga cgaccggggc gtccatctcc gcatcgagca ggacgcgtcc
     7201 cgtgtcgtgc aacaggccga tggcccagcg tccgaccagg atcgtcccgg cgatccccat
     7261 gacggggtcg agccaggtgg cgccgaaata cttgccgccg agcagcgccc cgattgcgag
     7321 tacggaggtt gccgcatcgg cgatcacgtg cacataggcc gagcgcaggt tgaggtcgtg
     7381 gtgatgcgcg tgggacgcgt gatggtcgtg gtcgtggtca tggccgtgcg agtgcgtgcc
     7441 ggccgacagc agccacgccg agaccacgtt gacgacgagg ccgacgaccg ccaccacgat
     7501 ggcctggtcg aagtggatcg gcgccggcgc aagcaggcgc ttgacggact cgtagcccat
     7561 gtacgcggcg acgcccacca ggaatacggc gctcgtgtaa ccccccagca cctcgatctt
     7621 ccaggtgccg aacgagaaac gaggatcgtt cgcatgccgg cgcgcgagcg cgtaggcacc
     7681 ggccgagacc ccgagcgcga gggcatgcga actcatgtgc cagccgtcgg ccagcagcgc
     7741 catggagccg aagacggtcc cggcgatgat ctcgatcacc atcatgacgg cggtgagcca
     7801 catcacctgt cgcgtccggc gctccgccac gggattgtgg aggtcgtagc ggtggacgtg
     7861 cggatgctct gggtgggact gcatgacgag cctgcgttcg cgtggccgcg gccagtatag
     7921 cggcgtcaat cggcacgcgg ccggcggcaa tgggcacgcg acccgatccg ttctatgctc
     7981 tcgactgcct gcgactgcgg ccggcggtcg tcaataacga gcgcaaatca tgagtgaccc
     8041 gatcaagtac ctcctcgacg aatcgcgcat cccgaagcac tggtacaaca tccaggccga
     8101 cctgccgaaa ccggccccgg cggtgctgca tcccggcact cacaagccgg tcggtcccga
     8161 cgacctcgcg ccgctgttcc cgatggaact gatcctgcag gaggtcaccc aggaacgcga
     8221 ggtgccgatc ccggaacccg tgcgcgacgt gtaccggcag tggcggccgt cgccgctgta
     8281 ccgcgcgcgc cggctcgagc aggcgctggg cacgcccgcg cgcatttact acaagtacga
     8341 aggcgtgtcg ccggccggca gccacaagcc gaacaccgcc gtgccgcagg ccttctacaa
     8401 caagcaggcc ggcgtgaagc ggctcatgac cgagacggga gcgggacagt ggggctcgtc
     8461 gctcgccttc gccggcgcgc tgtacgatct cgaggtgcag gtcttcatgg tgcgagtctc
     8521 gtacaaccag aagccgtacc gccgcgcgct gatggaaacc tacggggcgc gctgcgtcgc
     8581 atccccatcg agcgagacag agtctggacg cgcgatcctc gcgcaggacc ccaatcatcc
     8641 cggcagcctc gggatcgcga tctcggaagc ggtcgagctg gcagcccagc gcgacgacac
     8701 gaagtacgcg ctcggcagcg tgttgaacca cgtgctgctg caccagacgg tgatcggaca
     8761 ggaagcgatc gcccagttcg agatggcgga cgactacccc gacgtcatcg tcggctgcac
     8821 cggtggcggc tcgaacttcg ccggcatcgc attcccgttc atcggcgagc aactgcgggg
     8881 cggcaggaag gtgcgcgtcg tcgcggtcga gcccgcggcg tgcccgagcc tgacgcgtgg
     8941 cacctatgcg tacgatttcg gcgacaccgc gcacctgacg ccgctcacca agatgcacac
     9001 gctgggatcg accttcacgc cgccgggctt ccacgcgggc ggactgcgtt accacggcat
     9061 ggcgccgctg gtgagccacg tgaagcaact cgggctgatc gatgccgtcg cctaccagca
     9121 gacgggtatc ttcgcggcgg gcgtgcagtt cgcgcgggcg gaggggatcg tgccagcccc
     9181 tgaagcgaac catgcagtca agggcgcgat cgacgaagcg atgcgctgcc gcgaagaggg
     9241 cgtgtcgcgc gcgatcctct tcaacctctg cggacacggt cacttcgaca tgcaggcgta
     9301 catcgactac tttgcgggca agctcgagga ccgggactac gacccgaggg aactcgcgat
     9361 ggcgctcgcg ggcctgccgt cggtggccga gccggcctga cagaatcgcg cgaggaacga
     9421 gaccgggcag tcggcaaagg ggagggaacg atgcaaatgg atgcggtgga tgcacgtcag
     9481 catgctgcca gcgtcgcccc cggctgggca ctggccctgc gccgctacaa cgaccgcatg
     9541 atgttcgacc tgctgggcgg tccgcggccg tggaagctcg cgtgggtgat cgatgcgcag
     9601 aagtgcggca cgttccccgt gctggcgctg ctgatgtggt actactcggg aaggactccg
     9661 gcggcgacct cgccggctgc gtggacgtat ctcgccctgc acggatccta cggcctcgtc
     9721 tggctgctca aggacctggc gttcccggat cccaactggc agcgccgcgc gaccgtcgcg
     9781 agcgggctct acacgttcgc gggactcgtc ctgtactggt cgttcggctg gctgatcatc
     9841 tcgggaacgg tgatccccga ctacccgttg ccgcgggcgg tctggttcgc gctgtgcacg
     9901 accctgtgca tcctcggctg cgcgatcatg atcgcggccg atgcccagaa gtactttacg
     9961 ctgcgtgttc gacgcggact catccgcgac ggcgtgcatc gctacgtccg ccatccgaat
    10021 tacctcggcg aaatgctggt gtacgggtcg tttgcgctgc tcgtctggca ttggtggccg
    10081 tggctcgtgc tcggctggat ctggctgacg ctcttcgcga ccaacatggt catgaaggag
    10141 gcgagcctgt cccgccatga aggctggacg gactacaagc gccacagctg gtggctggtg
    10201 ccgttcgtgc tgtagccctc gggcgagggg caatcgcgcc tcgaatcgcg aacccgtgcg
    10261 ctggtcgtcc ggatgcgggc gacggacttc caggtcgaca gaaaaacggt aggattcggg
    10321 aagggccgca gggtcggtcg ttcagccagg agcaaggtgt cttgcgcaga ttcgtcagcg
    10381 ttttcgcact gctgtgtgcg gccgtaacgt cgttcgctgt tccgttctgg ggcgccaggg
    10441 actcgagtcc cgccgggacg ccgccagcag cgctcaagcc gggcgaattc gtctgggccc
    10501 ccgacatcgc accggacggc ccgatcgtcg tcatcgtgag cctcacggag cagcgtgcat
    10561 acgtctatcg caacggcgtg caggtcggat acaccaccgt gagcaccggg aagccggggc
    10621 acgagacgcc gacgggaatc ttcacgatcc tgcagaagga caaggaccac cggtcgtcca
    10681 agtacaacga tgcgccgatg ccctaccagg agcggctgac gtgggacggt gtcgcgctgc
    10741 acgccggcgg cctgcccggc taccccgagt cgcatggctg cgtgcacctg ccgtccaggt
    10801 tcgccgagga cctgttcagc atcacgcacc tcggcatgac ggtcgtggtg gtcaacgaca
    10861 tgaccgcgcc ccaggacgtc gtgcatccgg ccgcgctggt tccggtcagc gcctcgagcg
    10921 gcgaggcgga gatccagtcg cggctggtgg taggcgagaa gtggcgctgg gagcccgaga
    10981 agtcgcccga aggtccggtc tccatgatga tgagtgcggc ggaccagcgc atgatcgtgc
    11041 tgcgcaacgg gatcgagatc gggcgatcgc gggtctacat cagcgatccc gacgcgccgc
    11101 tcggcacgca tgccttcatc gtcaaggcgg gtgagggcag cggcgagagc gtgatcctca
    11161 agggcgcagc ggcccgcaac tggatggann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11281 nnnnnnnncg cggtccggcg cgcgtcagtg ctgcagtttg cagcgcatct ccggggcgcc
    11341 gtagccccag gtcacgctcg ctacgccctg gtgctcccag aaggactcgt tgcgaccctg
    11401 gtagcgagcg ccgctgccgc tcggctgcac gaacatgagc gagacgctgt cgccgcgctc
    11461 ggcgatcagc gtcggcggat cggtgcggaa atgcgtgacg acgacctcgc caccggcggg
    11521 ggcgtcgcag acgaacgtga aaggcccggt gaaggacacg agcctgtagc gcgcctgaag
    11581 ctcggcgatg cgtagcctgt attcctgctc cacgcaggcg cgccggtcat cgctcttcca
    11641 gcaatcgtcc cgtcccttga tccacccgcg ctgctcggcc ttgagcacag gtgggtgctc
    11701 gtcgacggcc ttcttcgaag cgtcggcata gacctgcgcg agctgccggt cgagcgcgga
    11761 caggccgctg tcagcgcaga tcaggctcgc gatgctcccg gcttcgaccg tgcggcagtc
    11821 gaaggacggt ccgtcggcgg cggcaactcc tggggcgggt gccagggccc acgccaacag
    11881 tccgccgacg atgcacacga cactgcgccg cgatgacatg ggttcccctc ggcgatcggt
    11941 ctggctcgat gatacgccgc gacggccgag gcgcgcaggc atgcgcaggt tcgcgcatgg
    12001 aaatggttga cgccacgtcg cctacactcc gggcgtcgtg cgaagtaagc tcgaccgagt
    12061 gacttaccag gggagtggac catggacatc gcccgggaat tgcgcgcgct gatcggcgag
    12121 tccggggtcc tcgatgcggc cgacgtcgcc aggcgctcgg caggcgtctg gaggccggac
    12181 aatctgcgcg ccgcggcgct ggcacgaccg accagcaccg agcaggtcgc cgccgtgctg
    12241 cgctggtgca atgcgaacgg cgtcccggcc gtcacgcagg gcggtctgac cggcctcgtg
    12301 cacggtgccg acgccggtcc cgacgaagtc atcctgtcgc tcgagcggat gcggaccatc
    12361 gagaacatcg atccggtgca gcgcaccgcc agcgtgcagg ccggagtcac gctcgaggtc
    12421 ctgcaggacg cggccgacgc ggcgcagctg ctgttccccg tggattttcc cgcgcgcggc
    12481 accgcgacga tcggcggcat ggcggcgacc aacgccggcg gcaatcgcgt catccgctac
    12541 ggcatgacgc gcgatcaggt cctcggcctg gaggtcgtgc tggccgacgg cacgatcgtc
    12601 tcgtcgctca actccctgat caagaacaac acgggctacg acctcaggca gctcttcatc
    12661 ggctccgagg ggacgctcgg cgtcatcacg cgcctggtgc tgcgcctgcg cgagcagccg
    12721 acgacggaaa acaccgcgtt tgtcgggcta gagagcttcg acagcgttgc aaagttcctc
    12781 aagcacatgg atcgcagcct cggtggcacg ctgtcgtcct tcgaggtcat gtggcggtcg
    12841 ttctaccggc tcgtgacgac tgctcccgcg aagggacgac cgccgatcgc gcaggactat
    12901 ccgttctacg tcctggtcga aggacagggt gccgatcgcg aactcgacac ccggcgcttc
    12961 aatgccgcga tggaatccgc cttcgagtcc ggcctggtcg tcgacgcggc catcgcacag
    13021 tccgcggcgg attcggcgag cttctgggcc cttcgcgacg acgtcgagca ggtgctgcac
    13081 ggaggactcc cggtcatctt cgacatttcc ctgccgatca gcgagatgga aggatttgcc
    13141 acgcgactcg aggagggatt gccgcgggaa atcggcgagg atcatcgcct gttcatcttc
    13201 gggcacctcg gcgacggcaa cctgcacgtg atcgtgcagg tgcaacccgc ggcctacgcg
    13261 gcggcgcgcc cgaaggtgga agcactggtc tatcgcggtc tcgaggaatt ccgcggcgcg
    13321 gtctcggccg agcacggcat cgggctggaa aagaagccgt ggctgcgggt gagtcgttcg
    13381 gaggccgaaa tcgccgtcat gcgcaggctc aaggccgcgc tcgatccgca caacttgctg
    13441 aatcccggca agatcctctg acggccggcc gtctcgggcc tagacccgct gcagcagcgc
    13501 gtaggcgaag gactgctcgc tgcccccggg cgtcatgtgc cgctcggcgc gcttggctac
    13561 cagcgtgaac gatgggacga acaacgctgc gatgtcctgc gccgaacggc gtgcgaccgg
    13621 caagccactg caccgttccg ggccgtccgg cgcaaatccg ccgatgatcg cgtgaccgcc
    13681 gcggcgcacc gcggcggcgg cctgttccgc ataccttgca gcgtccgccg gatcggtcag
    13741 gaaatgaaag accgcacggt cgtgccagat gtcgtacccg ccggccggga gtgcggcatc
    13801 gagcacgtcc gacacgagcc acgtcacctc gcgttcattc ggcccgatgc gccgccgagc
    13861 gattgccagc gcctctgccg agacgtcgag gacggtgagg cggcgcagcc cctggtcgag
    13921 cagatcgtcg acgagcgtcg acgcgccgcc gccgacgtcg atcaggcgag aatccgtgca
    13981 caggccagcg aggcccagca gttccagcga tacgtccagg cgcggccggt accagctgac
    14041 ggagtccgcg ctcttcgtca ggtagacctt ctgccagtgc gcctgccgac cggatggctc
    14101 gtcgggcatg tgtcgtatcg ctgagcctgc gagccgcgtt gtcaggggca cgtcgcggag
    14161 ccgggtacct tgcccgcgag cttcaggaac ccgccgaacg gtcccatgtt gcccatggcc
    14221 tcgcccatcg gcccgctgaa gtgcagtcga ccgaacatca tcgccttcat cggcccgtac
    14281 tcgccggcgc ccatctggtg ccagcgctcc gtggtcgcgt gcatcacgta gtcgacgtcg
    14341 ccatcgagct cttgcgtctc gaccgggccg ccgtagatgc attgcgcctt gtcgtccttg
    14401 agcgcgatgc gcaactcgac cgtgggcttg tcgccgcagt cggaccggta cagttgcagg
    14461 accttgtagc cgtggccggc gtcgttcttg acccacttgc tcttcacgag ctcggtggtc
    14521 agcacggggt cctggttcca ggcatcgcac gccgcgacgc cccatgggcc ggacatgaag
    14581 gccggctcct ccgccagcgc gggcacgcag gtcacggcgg ccaatgccgc cagcacgcgc
    14641 atcaacttca cttcgcgtag tctcatgctt ccggtctccg ggtttgcagc tccaggccag
    14701 cggcgtgccg ctcgcgcgga cgcaatacgt gcactgcgca tgcaatgcat gggtcgaagc
    14761 tgtggatcgt gcgcaggatc tcgatcggct gcgccgggtc gtgcagctcg tggccgacca
    14821 gcgccgcctc gtatgcaccc ggctgcccct gtgcatcgcg cggcccggcg ttccacgtgc
    14881 tcggcacgac ggcctggtag ttcgcgatct tctcgtcctc gatcacgatc cagtggccga
    14941 gtgcgccgcg tggggcctcc atgaagccgg cgccccgcgc cgtcttcggc cacgtggacg
    15001 gctcccacat cgagtcgtcg aacgtgcgcg tgtcgccggt cttgatgttg gcgatgagct
    15061 ggtcgtacca gccctgcatc gcgtcggcga agatcttcgt ctccagcgtg cgcgccgcgg
    15121 ttcggcctag agtggagtag agcgcctgca ccggcacttc cagcttcgcc agcgtcgagt
    15181 caaccagttc cttcgcctgc gcgttgccgg tcgcgtacat catcagcatg cgcgcgagcg
    15241 gtccgacctc gacggccttg cccttccagc gcggcgactt gagccagctg tactgcttct
    15301 cgacatcgag gaactggtac ggaggcttcg ggcccgtgta atcgagcttc gtctccccgt
    15361 cgtaaggatg caggcccgcc tggctgccgc cgccgtagtc gtaccaggcc cgcgcgacga
    15421 attcctggat ctgcgtgggg tcgtgcaggt ccacctcgtg gatcgtcgag aggtcgcggt
    15481 ccaggatcac gccgcgcggc acgaggaagg tcttcgggtc attgattccg tgcgacggga
    15541 aatcgccgta gcacatgaag ttcccgagcc cttcgccctg cgcggcccag tccttgtaga
    15601 agccggcgat cgcaagcgtg tccgggacgt acacctggtc cacgaactcg cgcatgcgaa
    15661 cgatcagttc ctggacctgc gccaggcgct tggcattgat cgccgagtcg gagtcgaggt
    15721 cgatcgggtt gggcacgccg ccaaccagga aattggggtg cgggttcttg ccgccgaaga
    15781 tcgtgtgcag ctccgcgacg tcacgctgcc aggcgagcgc gtcaagatag tgcgcgaccg
    15841 ccatgaggtt cgcctccggc ggcagcttgt aggcggggtg gccccagtag ccgttcgcga
    15901 agatgccgag ttgcccgccc tccacgaacg tcttcaagcg cttctgcacg tccgcgaagt
    15961 agccgggcga cgagcgcggg tagccggaca gcgactgcgc gagctgcgag gtagcagcgg
    16021 ggtccgcgcc gagggccgac accacgtcca cccagtcgag cgcgtgcagg tggtagaagt
    16081 gcatcacgtg gtcgtggatg ta
//
LOCUS       DAOMFD010000028        16036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_769_length_16036_cov_33.566423, whole genome shotgun sequence.
ACCESSION   DAOMFD010000028 DAOMFD010000000
VERSION     DAOMFD010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_769_length_16036_cov_33.566423"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="PL152_03115"
     CDS             complement(<1..272)
                     /locus_tag="PL152_03115"
                     /inference="COORDINATES: protein motif:HMM:NF023834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase N-terminal
                     domain-containing protein"
                     /protein_id="HQR48296.1"
                     /translation="MYSYRAPLRDIRFVVHEVLDIEDHYRSFGREDLNKDLLEGILEE
                     GARFAETVLAPTNRVGDEQGLRFEDGKVYTPPGFKEAYDRYCQDGW"
     gene            432..770
                     /locus_tag="PL152_03120"
     CDS             432..770
                     /locus_tag="PL152_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48297.1"
                     /translation="MSRDYDPQPGQWYEDLETEEVFKVLSVDPDEELVRIQWLDSEVE
                     DIDLDDWNEMDLELADEPEGWVDDEEEEEEEEEEEDDEEDWDEEEDDDWDDDEDEDYD
                     DEDDEYRDRE"
     gene            767..1345
                     /locus_tag="PL152_03125"
     CDS             767..1345
                     /locus_tag="PL152_03125"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR48298.1"
                     /translation="MSDTPALAQGTPAYPAELERVWQSADGTPVTLRALRPDDLDREL
                     AFIAGLSEETLHMRLQYSARGVNREEAARLLQLDYHDTFAMGALVAQGPDQRLVGVSR
                     YARVPDTDRAECAIVVADDWQGRGLGTELMRSLGTAARARGILALEGTILGENQRILA
                     WAHRFGFTVTAEPNSGGMLKVTLDLGSFPSLS"
     gene            complement(1302..2195)
                     /locus_tag="PL152_03130"
     CDS             complement(1302..2195)
                     /locus_tag="PL152_03130"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HQR48299.1"
                     /translation="MSAGSRQLAAALQGQQARLVSIAGRLGSVGGVDVHDARVAARRL
                     RSMLATFRPLLDADAAKRMRRGLRTFARGLTTVREADVRRDLLRQLLQEDPEVAPADA
                     ARVDALLGASIAAARAGVHREVAAPGWIANLASLADAEALDALLVRRDAGKDEVLQLA
                     HRPWRRARKLVARDPRDARELHRLRLALKHCRYAFEAVATCSPTRAAQVLKKLRAAQD
                     SLGEHRDVLQARDWVREHAASLGAPLTTVIEQSLRHRDRALRREAVERATDVMPAYAG
                     WRTATRRLRKGTGTTRGRASP"
     gene            2306..3667
                     /locus_tag="PL152_03135"
     CDS             2306..3667
                     /locus_tag="PL152_03135"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003904 - deoxyribodipyrimidine
                     photo-lyase activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HQR48300.1"
                     /translation="MGEPATAIVWFRRDLRLADNPALSAACDRADRVVLLYVHAPGEE
                     GDWPPGAASRWWLHHSLERLAGSLRARGAVLHLRHGPSLEAIVDVARSTGADSVFWNR
                     LYEPVIAQRDARVAAALRAAGITVETHNAATFVEPWESLTRDGRGYRVFTPFWRTHQA
                     QLDAQPSPLPVPRIRPVRLDGTNPPLDEFGLLPKVRWDAGLHAAWTPGEAGALARLDE
                     FCESIACGYTISRDRPDQDGTSRLSPHLHFGEVGPRQCLAAVRAAMAGRPGCQPGSEA
                     FLRQLGWREFAIHVLHHNPETVIEPLDRRFAKFAWDANPTLVQAWQQGRTGYPLVDAG
                     MRQLWETGWMHNRVRMVAASLLTKNLRQHWVDGARWFWDTLVDADLANNTLGWQWVAG
                     CGADAAPYFRIFNPQLQAERHDPTGAYVRRWLPDHGDSRPPLVDFKQSRAAALAAYAR
                     LKR"
     gene            3770..4255
                     /locus_tag="PL152_03140"
     CDS             3770..4255
                     /locus_tag="PL152_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQR48301.1"
                     /translation="MSEAGIYEGLASSKLAVELNEAERRTLAHAMTLRDLRHGEVLVQ
                     EGTADDHLYVVVSGVLGVVKNVGSDNEVTLNAKRPGDVVGELSFLDSSTRYASLVALS
                     DTRVLGLSRDALEGLLESHPKVVYHVMRAIVRVVHDIQRRLSMQTAELTNYLYKTHGR
                     Y"
     assembly_gap    4374..4473
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4474..5559
                     /locus_tag="PL152_03145"
     CDS             <4474..5559
                     /locus_tag="PL152_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR48302.1"
                     /translation="AGCILADAQAAAARADRYFPLALGSEGSCQVGGNLSTNAGGLNA
                     LRYGVTRDLVLGLEVVLADGRVLDGLTALRKDNTGYDLRDLFIGAEGTLGVITAASLK
                     LFPALRTVETAFVAVTGPAAAVQLLARLRAATGDALSSFELLPRIAIDLATTHIPGVT
                     DPLDRRYECYVLCEVSSARDDPGLRLALEGGLGRAIEDGLVLDAAFAESGAQRRALWR
                     LRESVPEAQRAEGASIKHDVSVPIAALPDFVAAASAAVLAIVPGGRMVSYGHVGDGNL
                     HFNVTEPLGGDRAAFLECEPRIQAAVQEVVRRFRGSISAEHGIGRLKRAQLAAHEGPV
                     AMDVMRAIKRALDPNGIMNPGKVLPDA"
     gene            5734..6423
                     /gene="aqpZ"
                     /locus_tag="PL152_03150"
     CDS             5734..6423
                     /gene="aqpZ"
                     /locus_tag="PL152_03150"
                     /inference="COORDINATES: protein motif:HMM:NF003838.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015250 - water channel activity [Evidence
                     IEA];
                     GO_process: GO:0006833 - water transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HQR48303.1"
                     /translation="MSKKLAAEFIGTFWLVLGGCGSAVLAAGVADVGIGYAGVALAFG
                     LTVLTMAFAIGHISGCHLNPAVTVGLWAGGRHPIGEVVPYIIAQVLGAIVGAYVVYMI
                     ASGQPGFDLNAGLASNGYGDHSPGGYSMAAAFLCEVVMAMMFLFIILGATDRRAPAGF
                     APIAIGLGLTLIHLISIPVTNTSVNPARSTGPALIVGGWAIAQLWMFWVAPVFGALIA
                     GYLYRWLAESK"
     gene            6487..9621
                     /locus_tag="PL152_03155"
     CDS             6487..9621
                     /locus_tag="PL152_03155"
                     /inference="COORDINATES: protein motif:HMM:NF017197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF748 domain-containing protein"
                     /protein_id="HQR48304.1"
                     /translation="MGGLLRSKWVRGIALAAALVGLYAFLGFRVAPELVRSQAISYVK
                     QQYGRDLRIGEVRVNPFLLQLEVRDLSLPDADGGPMIAFRRLFVDFEASSLWHRAYVF
                     KEVALEAPQLKTVIRPGGNVNLADLAPKETVPPEKAQPSGGLPPVWLHSLAVSGGAIG
                     FSDRNRAAPFERRFAPVNFTLKDFRTTAEGGGFQLSAQTPAGEGFAWKGRFEVAPAIG
                     SQGDLSIHGLRAQGISEYLADALPFILSSGQIDVAGHYDISLAQGLKAKVDLSKIEVS
                     GLAVRARGSDSDWIEIPGITVTDTSLALPARTISIAGVTVTDMQVQAWMAPDGSINLK
                     QLFAPQGAGPGADAGSAAPSVETTATPASAHAGATPVRLTTTGEPPPLQLSVGSAEVR
                     NATINFEDRLKEPVKQFVVTPLNIRVSNASLDLARPVAVSLDAVVNGVAPFRAEGTVT
                     PSPLAADLSVSLSGARMQILQPYVLPVADLTITDGRLTVDGRVKLDPPESPGPDLRFD
                     GDVSIDDFASIDNALKQDLVNFKRLEVRKISYAMGPDSATVEQVLVQQPFARVIISPE
                     RVVNISAVLDPEGTRKALEERRAAAAAEAAMTPAQKAEREREAEAAQERAEKQRKADR
                     VPVEAMAPLPAEGMPIRIREVRVARGTLDFTDHNVQPNFSARIQDVQGTVTGLSSDPK
                     SHAKLDFKGKVGDFSPVTITGEVQPFSFERYADIGLRFENIPLPIFNPYSGRFAGYAI
                     AKGKLTTDLHYLIQDRKLDAKHHIRIDQLEWGEQTATQGEATLPVKFATVLLRDVDGV
                     INLDIPVAGTLGDPTFRVGPIVWQIIKNLIVKAVTAPFRLLGALFAGAEEAQYVDFAP
                     GQAALDPQTVERLGSLSKSLLQKPGLNLDVPIGALPDLDGPALRESAFQEQLGKAMRK
                     ELGKGAKEGELPSYESLDPEQKIDVLNSLLEKQTGSRPQVPEPPAPPEGTSRKDAKAM
                     EQAAEIEFLETTARANVTVPATAIGELSEARATAIQHALVDTSGLDPARVFLVRNDKV
                     AAQDGKVRLQLELK"
     gene            complement(9676..10527)
                     /locus_tag="PL152_03160"
     CDS             complement(9676..10527)
                     /locus_tag="PL152_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HQR48305.1"
                     /translation="MNRLLADLVALLDLERLEMNLFRGESRDIGSPQVFGGQVLGQAL
                     RAAYGTVDDRKVHSLHAYFLRRGDFNAPIIYEVDRSRDGASFSNRRVLAIQHGQPIFN
                     MAASFQVEEDGPDHQVPVPDVAPPEELPDLTFEVRKHLARMPAERRKFWQLERPFEFR
                     SVEAIQAGEPSPGVRNLWFRLIDRCPDDPIVHHCLLAYMSDYHLLETATLPHGLAYRP
                     EDVTLASLDHGMWFHRPVRVDEWLLYALISPSSSGSRGLAFGRIFDRAGRLVASTAQE
                     GLMRTRG"
     gene            complement(10553..11617)
                     /locus_tag="PL152_03165"
     CDS             complement(10553..11617)
                     /locus_tag="PL152_03165"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR48306.1"
                     /translation="MSPTEPAAPGAAGEPIATDALVIGAGPAGLFQVFELGLLGVKAH
                     VVDTLRHPGGQCAELYPDKPIYDIPALPVCGAQELVDRLLEQIKPFGATFHFGQEVVS
                     LSRQGDGTFLVGTSAGTRFLARTVVVAAGLGSFQPRRLGVEGADAFEDRQVHYRVRNA
                     ADFHGQRLVIFGGGDSALDWVLDLHGKAKSIVHVHRRPEFRAAPASVARMKDLVAAGA
                     MTYVEGTAAALLKDGDRLVGITVKRSDGSTVDLEADHVLAFFGLHPKLGPIAEWGMEL
                     DKKALKVDTAKFQTSVPGIFAIGDINTYPGKKKLILSGFHEAALAAFAIAEHLNPAKK
                     VHLQYTTTSPIMQKRLGVSE"
     gene            11880..13277
                     /gene="hemN"
                     /locus_tag="PL152_03170"
     CDS             11880..13277
                     /gene="hemN"
                     /locus_tag="PL152_03170"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HQR48307.1"
                     /translation="MNSHQIHFDADLIRRYDKSGPRYTSYPTAVQFTDRFGLEEYKAA
                     AIASNQDPIPRPLSLYVHIPFCSSPCFYCGCTKVITRNHEKAEAYLYRLHREIELQGQ
                     LFDRDREAEQLHFGGGTPTFLTTLEIERLIEKLGNHFKLSSDPGREYSIELDPRTITR
                     DTLRDLARVGFNRCSLGIQDFDPAVQEAVNRIQSVPDTLRLIREAREFGFGSVSVDLI
                     YGLPNQTLEGFAKTIDTVLSARPDRFAVYGYAHLPQMFKAQRQLNADLLPTPETRLQL
                     LGLTIAKLSDAGYVYIGMDHFALPQDELVLAQENGTLHRNFQGYSTRGYCDLVALGVS
                     SIGKVGDTFAQNLKTLPEYYGALDRGELAVHRGLRLTRDDVIRRDVIQQIMCYGVLDF
                     DATGKRHGIDFRAYFASEIAALAPMVADGLAEFAGPGLRVLPAGRLLLRHLAMAFDAY
                     LASAQAAQQRFSKAI"
     gene            13508..15799
                     /locus_tag="PL152_03175"
     CDS             13508..15799
                     /locus_tag="PL152_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HQR48308.1"
                     /translation="MTNTSRLWKVLGAMLVASFGILLYMGHQIYLAAPPVPSEFRTTT
                     GRTLYTRADIDEGREVWQSIGGHELGSVWGHGSYVAPDWSADWLHREATGVLDIWAQR
                     ETGRRYAALDDATQSSMRAKLQPELRTNTYDPDTKVVTVTEDRAKAIAAVADHYIRLF
                     GNDPGLDTLRAQYAMQQDPIPDAGRRHALTAFFFWSAWSTVTNRPGESVSYTSNWPSE
                     PLVGNTPTAPTFLWTFISILGLLAGVGALAWYYVATHGKEPAPKVPDSDPLKSLTPTP
                     SMKATAKYFWTVTALFLVQVVLGGITAHYAVEGQSFYGLPLSDWLPYSVTRSWHLQLA
                     VLWIATAWLATGLYIAPAISGHEPKFQRLGVNFLFVCLLVIVVGAFAGQWLGVMQKLG
                     YETNFWFGHQGYEFVDIGRFWQAFLFVGLMLWLLLVGRALWPALKVRDDNRSITMLLF
                     LSTIAIGLFYGAGLMWGRHTHLAMVEYWRWWVVHLWVEGFFEVFATAVIAFLFTRLGL
                     VRPKTATNAVLFATIVFLSGGVLGTFHHLYWTGTPTSVLAIGSIFSALEVVPLVLIGF
                     EAYENYKHSHATPWVAAYRWPILFFVAVAFWNLVGAGLFGFLINPPLALYYMQGLNTT
                     PLHGHTALFGVYGMLGIGLMLFCLRGLGHLERWDERLLKGAFWTMNLGLATMALLSLL
                     PYGLMQVYAAVAHGFWYARSAEFLQQPTMQLLVWLRMPGDVLFSIGVLMLVAFVGKLF
                     IGRGRPQESRVPAGAQTAPTGRG"
BASE COUNT         2512 a         5514 c         5384 g         2526 t
ORIGIN      
        1 atccagccgt cctggcaata gcggtcgtag gcttccttga agccgggcgg cgtgtacacc
       61 ttgccgtcct cgaagcggag gccctgctcg tcgccgaccc ggttggtggg cgcgagcacc
      121 gtctcggcga aacgcgcgcc ctcctccagg atgccctcga gcaggtcctt gttcaggtcc
      181 tcccggccga agctgcgata gtggtcctcg atgtcgagga cttcatggac gacgaagcgg
      241 atgtcacgga gcggtgcgcg gtaggaatac acggtcttgc gtcctgtgat gtggcctttg
      301 ggccgattat atgcgatgca gcaaaatccg caggtaggtg tgttccggtt gccggttcgc
      361 gcgaggcgcc cgtatagtgg ccgcgccgcc acgatcctga aaatgctgga atttctgccg
      421 aggacgccgc cgtgagccgc gattacgacc cgcaacccgg gcaatggtac gaagacctcg
      481 aaaccgagga agtcttcaag gtcctgtcgg tggaccccga cgaggaactg gtgcggatcc
      541 agtggctcga cagcgaggtc gaggacatcg acctcgacga ctggaacgag atggatctcg
      601 aactcgccga cgagccggaa ggctgggtcg acgacgagga agaggaggag gaagaggaag
      661 aggaagagga cgacgaggaa gactgggacg aggaagagga cgacgactgg gacgacgacg
      721 aggacgagga ctacgacgac gaagacgacg agtatcgtga ccgcgagtga gcgacacacc
      781 tgccctggcc caaggaaccc cggcctaccc ggctgaactc gagcgcgtct ggcagtcggc
      841 cgacgggaca cccgtcacgt tgcgggcgct gcgcccggac gacctcgacc gcgagctggc
      901 attcatcgcg ggcctgtccg aagaaacgct gcacatgcgc ctgcagtact cggcccgcgg
      961 cgtgaaccgc gaggaggccg cgcggctgct gcagctcgac taccacgaca cattcgcgat
     1021 gggtgcgctg gtggcgcagg ggcccgacca gaggctcgtc ggcgtcagcc gctatgcgcg
     1081 cgtgccggac acggaccggg ccgaatgcgc catcgtcgtc gccgacgact ggcagggccg
     1141 cgggctcggc accgagctca tgcgcagcct cggcacggcg gcgcgcgccc gcggaatcct
     1201 cgcgctcgaa gggaccatcc tcggcgagaa ccagcgcatc ctggcctggg cgcatcgatt
     1261 cggcttcacg gtgaccgccg aacccaattc cggcgggatg ctcaaggtga cgctcgacct
     1321 cgggtcgttc ccgtcccttt cctgagccgt cgcgtggccg tgcgccagcc cgcgtaggcc
     1381 ggcatcacgt cggtcgcgcg ctcgaccgcc tcgcgccgca gcgcccggtc gcggtgcctc
     1441 agcgactgct cgatcaccgt ggtgagcggc gctcccagcg acgccgcgtg ctcgcgcacc
     1501 cagtcccgcg cctgaagcac gtcccgatgt tcaccgaggc tgtcctgcgc cgcgcgcaat
     1561 ttcttcagga cctgggcggc ccgggtcgga ctgcacgtcg cgactgcctc gaatgcgtag
     1621 cgacagtgct tgagcgccag gcggagccgg tgcagctccc tggcgtcgcg gggatcgcgc
     1681 gcgaccagct tgcgggcccg ccgccaggga cggtgtgcca actgcagcac ttcgtccttg
     1741 ccggcgtcgc ggcgcacgag cagcgcgtcc agggcctccg cgtccgcaag actcgcgaga
     1801 tttgcaatcc agcccggtgc cgcgacctcg cgatgcacgc cggcccgagc cgccgcgatc
     1861 gaagcgccca gcagtgcgtc cacccgtgca gcatcggcgg gtgcgacttc cggatcctcc
     1921 tgcagcagct ggcgcagcag gtcgcgtcgc acgtcggctt cgcgcacggt cgtgagcccg
     1981 cgagcgaacg tgcgcagccc gcgacgcatg cgcttcgccg cgtccgcatc gagcagcggg
     2041 cgaaaggtcg cgagcatcga tcgcagccgc cgggcggcca cccgggcgtc gtgcacgtcc
     2101 acgccgccca ccgatccgag gcgaccggcg atcgacacca atcgagcctg ctgcccctgc
     2161 aatgcagccg ccagctgccg actgccggcg gacatcgccc gcgcgcctgt tcgccgagct
     2221 tgcatgcctg cagcctagta gaattccgcg tttgccgcga ccgcatcgga gccgttcgag
     2281 agtcgagtcc cccgggagac caccattggg cgagcccgcc accgccatcg tctggtttcg
     2341 cagggacctg cgcctggccg acaaccctgc actgtcggcc gcctgtgacc gagccgatcg
     2401 cgtcgtgctg ctgtacgtcc acgcgcccgg cgaggaaggc gactggccac ccggcgccgc
     2461 gagccgatgg tggctgcacc acagtctcga gcggttggcg ggctcgttgc gtgcacgcgg
     2521 cgcagtgctg cacctgcggc acggaccgtc gctcgaagcg attgtcgacg tcgcgcgatc
     2581 caccggtgcc gacagcgtgt tctggaaccg gctctacgaa ccggtgatcg cgcagcgcga
     2641 tgcgcgcgtg gctgcggcac tgcgggcggc cggcatcacc gtcgaaaccc acaacgccgc
     2701 gacattcgtc gagccttggg aatcgctgac ccgcgacggc cgtggctatc gggtgttcac
     2761 gccattctgg cgcacacacc aggcgcagct cgacgcccaa ccgtcgccgt tgcccgtgcc
     2821 gcgaatccgc ccggtccgac tcgacggaac gaacccgccg ctcgacgagt tcggattgct
     2881 gcccaaggtt cgttgggacg cgggactgca cgcagcgtgg accccgggcg aggccggtgc
     2941 gctcgcgcgg ctcgacgaat tctgcgagtc gatcgcgtgc ggatacacga tctcgcgcga
     3001 ccggccggac caggacggca cctccaggct gtcgccgcat ctgcacttcg gcgaggtcgg
     3061 cccacggcag tgcctcgcag ccgttcgggc ggcgatggcc ggacgtccag gttgccagcc
     3121 gggcagcgag gcgttcctcc gccagctggg ctggcgcgag ttcgcgatcc acgtccttca
     3181 ccacaacccc gagaccgtca tcgagccgct cgaccgccgg tttgcgaagt tcgcctggga
     3241 tgcgaaccct acgctcgtcc aggcatggca gcagggtcga accggatatc cactggtcga
     3301 cgcggggatg cgccagttgt gggagacggg ctggatgcac aaccgcgtgc gcatggtcgc
     3361 ggcctcgctg ctcaccaaga acctgcgcca gcactgggtc gacggcgccc gctggttctg
     3421 ggacacgctc gtcgacgccg acctcgcgaa caacacgctc ggatggcagt gggtcgcggg
     3481 ttgcggagcc gacgccgctc cgtacttccg catcttcaac ccgcagctgc aggcggagcg
     3541 ccacgacccg acgggcgctt acgtgcggcg ctggctgccc gatcacggcg acagtcgacc
     3601 gccgctggtc gacttcaagc agagtcgcgc ggccgcgctg gccgcctacg cccgcctgaa
     3661 gcgatagcgg aatcccgcgg cactacgcac gaccgcgggt ttcacctact atcgcggcga
     3721 cgccctacgc ccgagaattc atgggtcgca tcgacccggg agcgcggcca tgtccgaagc
     3781 gggaatctac gaaggcctgg ccagctcgaa gctggccgtc gaactgaacg aagcggagcg
     3841 gcgcacgctc gcgcatgcca tgactctgcg cgacctccgc cacggcgagg tgctcgtgca
     3901 ggaaggcacg gcggacgacc acctctacgt ggtggtgagc ggcgtgctcg gtgtggtcaa
     3961 gaacgtgggc agcgacaacg aagtgacgct gaacgcgaag cgccccgggg acgtggtggg
     4021 cgagttgtcg ttcctcgaca gctcgacgcg ctacgcgtct ctcgtcgcat tgagcgacac
     4081 acgggtgctc ggtctcagcc gcgatgccct cgagggactg ctggagtcgc acccgaaggt
     4141 cgtctaccac gtgatgcggg cgatcgtgcg cgtcgtgcac gacatccagc gccggctctc
     4201 gatgcagacc gcggaactca ccaactacct gtacaagacc cacggccgtt actgagcggc
     4261 gacccgcgcg ctcccgactg gccgctgcca cacctgccgc gtacgatcgc gctcatgcct
     4321 gcgccttcgc agtccgcgat cgccacggcc gtggcgcgca tccgcgacgc gctnnnnnnn
     4381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngctggtt gcatccttgc cgacgcgcag
     4501 gccgctgccg cgcgcgcgga ccgatacttc ccgctcgcgc tcggctcgga aggaagttgc
     4561 caggtcggcg gcaacctctc gacgaatgcc ggcggcctga atgcgctgcg ttacggcgtc
     4621 acccgcgacc tggtgctcgg cctcgaggtc gtgctcgcgg atggacgggt gctggacggc
     4681 ctgaccgcgc tgcgcaagga caacaccggc tacgacctgc gcgacctgtt catcggcgcc
     4741 gaaggcacgc tcggcgtgat cacggctgcg agcctgaagc tctttcccgc gctgcgtacg
     4801 gtcgagaccg cattcgtcgc cgtcacgggc ccggccgctg cagtacagct gctggctcgg
     4861 ctccgagcgg cgaccggcga tgcgctctcg agcttcgaac tcctgccgcg gatcgcgatc
     4921 gacctggcca cgacgcacat accgggcgtg acggacccgc tcgaccgccg ctatgaatgc
     4981 tacgtgctct gcgaggtgtc ctcggcgcgg gacgaccccg ggctgcgcct cgcactggag
     5041 ggcggcctcg gccgcgcgat cgaagacggc ctcgtgctgg atgcggcgtt cgcggaatcg
     5101 ggcgcgcagc gccgggcgct gtggcgcctg cgcgagtcgg tgcccgaggc ccagcgcgcc
     5161 gaaggcgcga gcatcaagca cgacgtctcg gtaccgatcg cggcgctgcc ggacttcgtg
     5221 gcggcggcga gtgcggccgt gctcgcgatc gtgcccgggg gccggatggt ctcttacgga
     5281 cacgtgggtg acggcaacct gcacttcaac gtgaccgagc cacttggcgg ggaccgtgcg
     5341 gcattcctgg agtgcgagcc acggatccag gctgcggtgc aggaagtcgt ccgccgcttc
     5401 cgcggcagca tcagcgccga gcacgggatc ggccggctga agcgggcgca gctcgccgcc
     5461 cacgagggcc cggtcgcgat ggacgtcatg cgcgcgatca agcgcgcgct ggacccgaac
     5521 ggaatcatga accccggcaa ggtgctgccc gacgcgtaac cgtgagggtt gccgagccgc
     5581 agcgcccggg catgcctgca cggggcctcg gccaggtcca ggaagagccg cgtcataggc
     5641 aatttccctt gtggaagccg actggattcg ctaaatacga ttcaaactcg agtcggacga
     5701 ttccaacaac aacaggaggg cccggacatg cccatgtcga agaagctggc agcggaattc
     5761 atcggaacgt tctggctggt gctcggcggc tgcggcagtg cggtgctcgc ggcaggcgtg
     5821 gccgatgtcg gcatcggtta cgctggtgtg gcactggcct ttggtctcac cgtgctcacg
     5881 atggccttcg ccatcggcca catctcgggc tgtcacctga accccgcggt caccgtcggc
     5941 ttgtgggccg gcggacggca cccgatcggc gaggtcgtgc cttacatcat cgcgcaggtg
     6001 ctgggtgcaa tcgtcggcgc gtacgtcgtg tacatgattg cgtccggtca gccgggcttc
     6061 gacctcaatg cgggactggc ctcgaacggc tacggcgatc actcaccggg cgggtactcg
     6121 atggccgcag cattcctgtg cgaggtcgtg atggcgatga tgttcctctt catcatcctc
     6181 ggcgccaccg accgccgcgc gccggcgggg tttgcgccga tcgccatcgg cctcgggctc
     6241 acgctgatcc acttgatcag catcccggtc accaatacct cggtgaaccc tgcgcgcagc
     6301 actggccccg ccctgatcgt gggcggctgg gccattgcgc agctctggat gttctgggtc
     6361 gcaccggtct tcggcgcgct catcgccggc tacctgtatc gctggttggc ggagagcaag
     6421 tgagctgacg cgggtcgtcc ggcgatctcg tgtcgtcgga cgcccgacca gcgaggcacg
     6481 tgatccatgg gcgggctcct caggtcgaag tgggtgcggg gcattgcgct cgcggccgcg
     6541 ctggtcgggc tgtacgcatt cctgggattc cgggtggctc cggagctcgt gcgcagccag
     6601 gcgatctcct acgtcaagca acagtatggc cgcgacctgc gcatcggcga ggtgcgcgtc
     6661 aacccgttcc tgctgcagct ggaagtccgc gacctgtcgc tgcccgatgc ggacggcggc
     6721 ccgatgatcg cctttcgccg gctgttcgtg gacttcgagg cgtcttcgct ctggcaccgt
     6781 gcctacgtct tcaaggaggt cgccctcgag gcgccacagc tgaagaccgt gatcaggccc
     6841 ggcggcaacg tcaatctcgc ggacctcgcg ccgaaggaaa cggtgccgcc tgagaaagcg
     6901 cagccatccg gtgggctgcc gcccgtctgg ctgcattcgc ttgcggtgtc cggcggcgcg
     6961 atcggcttct cggatcgcaa ccgggcggcg ccgttcgagc gacggttcgc gccggtcaac
     7021 ttcacgctga aggacttccg cacgaccgcc gagggcggcg gcttccagct gtccgcgcag
     7081 actccggccg gcgagggctt cgcgtggaaa ggtcgattcg aggtcgcgcc ggccatcggg
     7141 tcgcagggcg acctctcgat ccacggactt cgggcgcaag gcatcagcga ataccttgcc
     7201 gatgccctgc ccttcatcct gagctcgggc cagatcgatg tcgccggcca ctacgacatc
     7261 tccctcgccc agggcctgaa ggcgaaggtg gacttgtcga agatcgaagt ctcgggcctc
     7321 gccgtccgcg cgcgcgggtc ggacagcgac tggatcgaga tccccgggat cacggtcacc
     7381 gacacgtcgc tcgcgttgcc ggcgcgcacg atctcgatcg ccggcgtcac cgtcaccgac
     7441 atgcaggtgc aggcctggat ggcgccggac ggctccatca acctcaagca gctgtttgct
     7501 ccgcaaggcg caggcccggg ggcggacgcc ggcagcgccg cgccatccgt cgagaccaca
     7561 gccacgcctg cgtcggcgca cgcaggggcg acacctgtgc gactcacgac gaccggcgag
     7621 ccgccgccgc tgcaactctc ggtaggaagc gccgaggtcc gcaacgcgac catcaacttc
     7681 gaggaccgtc tcaaggagcc ggtcaagcag ttcgtcgtca cgccgctcaa catccgcgtg
     7741 agcaacgcaa gcctggacct cgccaggccc gtcgcggtgt cgctcgacgc cgtcgtcaac
     7801 ggcgtcgcgc cgttcagggc cgagggcacg gtgacgccgt cgccgctcgc cgcggacctg
     7861 tcggtctcgc tcagcggcgc acgcatgcag atcctgcagc cctacgtgct gccggtcgcg
     7921 gatctcacca tcaccgacgg ccgcctgacg gtggatggca gggtgaagct cgatccgccc
     7981 gagtcgcccg gccccgacct gcgcttcgac ggcgacgtgt cgatcgacga cttcgcatcg
     8041 atcgacaacg ccctcaagca ggacctcgtg aacttcaagc gcctcgaggt gcgcaagatc
     8101 agctatgcga tgggacccga ctcggcgacc gtggaacagg tgctggtgca gcagccgttc
     8161 gcgcgcgtga tcatcagccc ggagcgcgtg gtcaacatct cggccgtgct cgaccccgag
     8221 ggcacgcgca aggcgctcga ggaacggcgg gccgccgccg cggccgaagc cgcgatgaca
     8281 cccgcccaga aggcggagcg cgagcgcgag gccgaggcgg cgcaggagcg ggccgagaag
     8341 cagcgcaagg ccgatcgcgt tccggtcgag gcgatggcac cgctgcctgc ggaggggatg
     8401 ccgatccgca ttcgcgaggt gcgcgtcgcg cggggcacgc tcgacttcac agaccacaac
     8461 gtgcagccga atttctcggc gcgcatccag gacgtgcagg gcaccgtgac cgggctctcg
     8521 agcgacccga agtcgcacgc aaagctggat ttcaagggca aggtcggcga cttctcgccg
     8581 gtgacgatca cgggcgaagt gcagccgttc tcgttcgagc gctacgcgga catcgggctc
     8641 cggttcgaga acatcccgct gccgatcttc aacccgtact ccgggcgctt cgccggctac
     8701 gcgatcgcca agggcaagct cacgaccgac ctgcactacc tgatccagga ccgcaagctc
     8761 gacgcgaagc accacatccg catcgaccag ctcgagtggg gcgagcagac cgcaacccag
     8821 ggtgaagcca cactgccggt gaagttcgca accgtgctgc tgcgggacgt cgacggggtg
     8881 atcaacctcg acatcccggt tgcgggcacg ctcggcgacc cgacgttccg cgtcgggccg
     8941 atcgtctggc agatcatcaa gaacctgatc gtcaaggcgg tgaccgcgcc gttcaggctg
     9001 ctgggagcgc tgttcgcggg agcggaggaa gcccagtacg tcgattttgc gccgggccag
     9061 gcggcgctcg atccgcagac cgtcgagcgg ctcggctcct tgtcgaagag cctgctgcag
     9121 aagccgggcc tcaatctgga cgtgccgatc ggcgcgctgc cggatctcga cggcccggcg
     9181 ctgcgggaga gcgcgttcca ggagcagctc gggaaggcga tgaggaagga actgggcaag
     9241 ggcgcgaagg aaggtgaact gccctcctac gagagcctcg atccggagca gaagatcgac
     9301 gtgctgaaca gcctgctcga gaagcagaca ggctccaggc cgcaggtccc cgagccacct
     9361 gcgccaccgg aggggacctc gcgcaaggac gcgaaggcga tggaacaggc ggccgagatc
     9421 gagttcctcg agacgacggc gcgtgccaac gtcacggtgc cggccacggc catcggcgag
     9481 ctgtcggagg cacgcgcgac cgcgatccag cacgcgctgg tcgatacgag cgggctcgat
     9541 ccagctcgcg tattcctggt caggaacgac aaggtcgccg cgcaggacgg caaggtgcgc
     9601 ctgcagctcg agctgaaata accggcggtc cgcgcgcttc cttgccgccg catccggcgc
     9661 gacgggtacg cgtcgtcagc cccgcgtgcg catgagtcct tcctgcgccg tgctcgcgac
     9721 cagccgcccg gcacggtcga agatgcgtcc aaacgccagg cctcgcgatc cggagctgct
     9781 cgggctgatg agcgcgtaca gcagccactc gtccacccgc accggccgat ggaaccacat
     9841 gccgtggtcg aggctcgcga gcgtgacgtc ctcgggccga taggcgagcc cgtgcggcag
     9901 cgtcgcggtc tcgagcaggt gatagtccga catgtaggcg agcaggcagt ggtgcacgat
     9961 cgggtcgtcg gggcaccggt cgatcagccg gaaccagaga ttcctcaccc ccggactcgg
    10021 ttcccccgcc tggatcgctt ccacggagcg gaactcgaag ggcctttcga gctgccagaa
    10081 tttgcggcgc tcggccggca tccgtgccag gtgcttgcgc acttcgaagg tgaggtccgg
    10141 caattcctcc gggggagcga cgtcgggcac cggcacctgg tgatccggcc cgtcctcctc
    10201 gacctggaac gacgcggcca tgttgaagat cggctggccg tgctggatcg cgagcacccg
    10261 gcgattggag aagctggctc cgtcgcggct gcggtcgacc tcgtagatga tcggcgcgtt
    10321 gaagtcgccg cgacgcagga agtacgcgtg caacgagtgc accttgcgat cgtcgaccgt
    10381 gccgtacgcg gcccggagcg cctgcccaag cacctgtccg ccgaacacct gcgggctgcc
    10441 gatgtcgcgg ctttcgccgc ggaacaggtt catctccagg cgctcgagat cgagcagcgc
    10501 cacgaggtcc gcgagcaggc ggttcatacc ggcagccagg ccgcggtcgc cgtcactcgg
    10561 acacgccgag ccgcttctgc atgatcgggc tcgtcgtggt gtactggagg tgcaccttct
    10621 tcgccgggtt caggtgctcg gcgatcgcga atgccgcgag tgccgcctcg tggaaaccgc
    10681 tcaggatcag cttcttcttg cccgggtaag tgttgatgtc gccgatcgcg aagatccccg
    10741 gcacgctggt ctggaatttc gccgtgtcca ccttgagcgc tttcttgtcg agctccatgc
    10801 cccattccgc gatcggcccg agcttcgggt gcagcccgaa gaatgcgagc acgtggtcgg
    10861 cctcgaggtc gaccgtcgaa ccgtcgctgc gcttgacggt gatgccgacc aggcggtcgc
    10921 catccttgag cagcgccgcc gccgttccct cgacgtaggt catcgcgccc gccgcgacca
    10981 gatccttcat gcgcgcgacc gacgcgggtg cggcgcggaa ttccggccgg cgatgcacgt
    11041 gcacgatgct cttcgccttg ccgtgcaggt cgagcaccca gtcgagcgcg gagtccccgc
    11101 cgccgaagat caccagccgc tgcccgtgga aatcggcggc attgcgcacg cggtagtgga
    11161 cctgccggtc ctcgaacgcg tccgcgccct cgaccccgag gcggcgcggc tggaacgagc
    11221 cgagcccggc ggcgacgacc acggtcctgg ccaggaaccg cgtccccgcc gacgtgccga
    11281 ccaggaacgt gccgtcgccc tgccgggaca gcgagaccac ctcctggccg aagtgaaagg
    11341 tcgcgccgaa gggcttgatc tgctcgagca ggcggtccac gagttcctgc gcgccgcaga
    11401 caggcagcgc cgggatgtcg tagatcggct tgtcgggata gagctcggcg cactggccgc
    11461 cggggtgccg cagcgtgtcc acgacatggg ccttgacgcc cagcagcccg agttcgaaga
    11521 cctggaacag gccggccggg ccggcgccga tgaccagggc atcggtggcg atgggctcgc
    11581 cggccgcgcc gggcgcggca ggttcggtgg gactcaaggc tgtatctccg tcaggcccgc
    11641 gggtcgggtg gaaattctag cggatctgtg cgccgcccca cctgccgtcc accgagggct
    11701 tgcgcgccac cgagacgcca ccgcgggaaa cccctcccta cccggcaccg gccggcgcgt
    11761 ctatgcttcg cgctgcaggg taggcggtgc ggcccggaac ctggcgcttg accggtgtca
    11821 atgcgcggat tccggggtag tggcatgctt ccctgcgccg accgagtaca gggcagccga
    11881 tgaactctca ccagatccac ttcgacgccg atctcatccg ccgctacgac aagtcggggc
    11941 cgcgctacac gtcgtacccg acggccgtgc agttcaccga ccggttcgga ctcgaggagt
    12001 acaaggccgc cgcgattgcg agcaaccagg acccgatccc gcgtccgctg tcgctgtacg
    12061 tgcacatccc gttctgctcg agcccctgct tctactgcgg ctgcaccaag gtcatcacgc
    12121 gcaaccacga gaaggccgag gcctacctgt accggctgca ccgcgagatc gagctgcagg
    12181 gacagctctt cgatcgcgat cgcgaggccg agcagctgca cttcggcggc ggcacgccca
    12241 ccttcctcac cacgctcgag atcgagcggc tgatcgagaa gctcggcaat cacttcaagc
    12301 tttcgagcga cccgggccgc gagtactcga tcgagctcga cccgcgcacg atcacgcgtg
    12361 acaccctgcg cgacctcgcc cgcgtcgggt tcaaccgctg cagcctcggc atccaggact
    12421 tcgaccccgc cgtgcaggaa gcggtgaacc gcatccagtc ggtgccggac acgctgcggc
    12481 tcatccgcga ggcgcgcgaa ttcggcttcg gctcggtgag cgtggacctg atctacggcc
    12541 tgccgaacca gacgctcgag ggcttcgcga agacgatcga cacggtgctg tcggccaggc
    12601 cggatcgctt cgccgtctac ggctacgcgc acctgccgca gatgttcaag gcgcagcggc
    12661 aattgaacgc ggatctactg ccgacgcccg agacgcggct gcagctgctc ggtctcacga
    12721 tcgccaagct gtcggacgcg ggctacgtgt acatcggcat ggatcacttc gccctgccgc
    12781 aggacgaact cgtgctggcg caggagaacg gcaccctgca ccgcaatttc cagggctatt
    12841 cgacacgcgg ctactgcgac ctggtcgcgc tcggcgtgag ttccatcggc aaggtcggcg
    12901 acacgttcgc gcagaacctg aagaccctgc ccgagtacta cggcgcgctc gaccgcggcg
    12961 aactcgccgt gcaccgcggc ctcaggctca cgcgtgacga cgtcatccgc cgcgacgtga
    13021 tccagcagat catgtgctac ggcgtgctgg acttcgacgc cacgggcaag cgccatggca
    13081 tcgacttccg cgcgtacttc gcgagcgaga tcgcggcgct cgccccgatg gtcgccgacg
    13141 ggctcgcgga gttcgccggg cccgggctgc gcgtgctgcc cgcgggtcgc ctgctcctgc
    13201 gccatctcgc catggcattc gacgcctacc tcgcgtccgc ccaggccgcg cagcagcgat
    13261 tctcgaaggc gatctgacgc gctgccggca agcgcgccgg agaggcgggt gagcgacggg
    13321 gggccggcca tgccggccct cattctttgc gcaggtcccg ctggtcatca ggccggccca
    13381 gctgcgggca attctcctgc gatcgtcctc cgacgtgttt gacctcgatc aaggcgaccc
    13441 ggtttgccgc actgcaccat tgcgtccgcc tgcggccccc gccgctgcgt gcaaaccgga
    13501 gagaccgatg accaatacga gtcgactgtg gaaagtgctg ggagcgatgc tcgtcgcttc
    13561 cttcgggatc ctcttgtaca tggggcacca gatctacctg gcggcgcctc cggtcccgtc
    13621 cgagttcagg accacgaccg gcaggaccct gtacacgcgc gcggacatcg acgagggacg
    13681 cgaggtctgg cagagcatcg gcggccacga gctcgggtcc gtctggggtc acggcagcta
    13741 cgtcgcgccg gactggtcgg cggactggct gcaccgcgag gcgaccggcg tgctcgacat
    13801 ctgggcgcaa cgcgagaccg gccggcgtta cgccgccctc gacgacgcga cgcagtcgtc
    13861 gatgcgagcc aagctgcagc cggagctgcg aaccaacacc tacgaccctg acacgaaagt
    13921 cgtcacggtc acggaggacc gcgccaaggc catcgccgcg gtcgcggacc actacattcg
    13981 cctgttcggc aacgacccgg ggctcgacac gctgcgcgcc cagtacgcga tgcagcagga
    14041 cccgattccg gacgccgggc gtcgccatgc gttgaccgcc ttcttcttct ggtccgcgtg
    14101 gtcgaccgtc acgaaccgtc ccggcgagtc ggtcagctac acgagcaact ggccgtcgga
    14161 gccgctggtc ggcaacaccc cgactgcgcc gaccttcctc tggaccttca tcagcatcct
    14221 cggattgctc gccggcgtgg gcgcgctcgc gtggtactac gtggccaccc atggcaagga
    14281 gcccgcaccg aaggttccgg acagcgaccc gctgaagtcg ctgacaccga cgccgtcgat
    14341 gaaggcgacg gccaagtact tctggacggt cacggcgctg ttcctggtgc aggtcgtgct
    14401 gggcgggatc accgcgcatt acgcggtcga aggccagtcc ttctacggcc tcccgctatc
    14461 ggactggctg ccgtactcgg tgacgcgttc ctggcacctg cagcttgccg tgctgtggat
    14521 cgcaaccgcc tggctcgcca ccgggctcta catcgcgccc gcgatctcgg gacacgagcc
    14581 gaagttccag cgtctcggcg tcaacttcct gttcgtgtgc ctgctcgtca tcgtcgtcgg
    14641 cgcattcgcc gggcaatggc tcggcgtcat gcagaagctg ggctatgaga cgaacttctg
    14701 gttcggccac cagggctacg agttcgtcga catcggccgc ttctggcagg cgttcctgtt
    14761 cgtcggcctg atgctgtggc tgctgctcgt cggccgcgcg ctgtggccgg cgctgaaggt
    14821 ccgcgacgac aatcgctcca tcaccatgct gttgttcctc tccacgatcg cgatcggcct
    14881 gttctacggt gccggcctga tgtgggggcg ccacacgcac ctcgccatgg tcgagtactg
    14941 gcgctggtgg gtcgtgcacc tgtgggtcga gggcttcttc gaggtgttcg cgaccgcggt
    15001 gatcgcgttc ctgttcaccc ggctcggcct ggtgcggccg aagaccgcga cgaatgcggt
    15061 gctgttcgcc accatcgtgt tcctgagcgg cggcgtgctc ggcacgttcc atcacctgta
    15121 ctggaccggc acgccgacct ccgtgctcgc gatcggctcg atcttctcgg cgctcgaggt
    15181 ggtgccgctg gtgctcatcg gcttcgaggc ctacgagaac tacaagcaca gccatgcgac
    15241 gccctgggtc gcggcctatc gctggccgat cctgttcttc gtcgcggtgg ccttctggaa
    15301 tctcgtgggc gcgggactgt tcggcttcct gatcaacccg ccgctcgcgc tctactacat
    15361 gcagggcctc aatacgacgc cgctgcacgg ccacacggcg ctgttcggcg tgtacggcat
    15421 gctcggcatc ggcctgatgc tgttctgcct gcgcggactc ggacacctcg agcgctggga
    15481 cgagcggctg ctcaagggcg cgttctggac catgaacctc gggctcgcga ccatggccct
    15541 gctctcgctg ctcccgtacg gcctgatgca ggtgtacgca gcggtcgcgc acggcttctg
    15601 gtatgcgcgg tcggcggagt tcctgcagca gccgacgatg cagctgctcg tgtggctgcg
    15661 catgccgggc gacgtcctgt tcagcatcgg cgtgctgatg ctggtcgcct tcgtgggcaa
    15721 gctgttcatc gggcgcggac gtccgcagga atcgcgtgtc ccggccggcg cgcagaccgc
    15781 accgaccgga cgcggctgag acgaaccgct ctgcccgtgc tgcgggtgcc gcgaggtacc
    15841 cgcagcacgt ccgtttcagg cttgccgtca cggcgcatac actgaggccc gggcatggaa
    15901 ccaggatcca cgatgacgtt gcgctggaac atcctcacgg cggcaccgca tcggctgctg
    15961 ttcttctgcg gcatgctgaa tctcgcgctc gccagcctct ggtgggtcgg gcatctcgtc
    16021 gcccgctaca ccggct
//
LOCUS       DAOMFD010000029        16025 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_771_length_16025_cov_28.664245, whole genome shotgun sequence.
ACCESSION   DAOMFD010000029 DAOMFD010000000
VERSION     DAOMFD010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 16025)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16025)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16025
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_771_length_16025_cov_28.664245"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(105..971)
                     /locus_tag="PL152_03180"
     CDS             complement(105..971)
                     /locus_tag="PL152_03180"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR48309.1"
                     /translation="MNFNHLRYFWAVAHAGGLTRAAERMHVSQSSLSVQIRKLEHQLG
                     HPLFERAGRKLVLTEAGRMALDYADTVFRAGDELLSTLRGRPAANRVVLSVGALTTLS
                     RNFQLEFLRPLVGRSDVELIVRSGSIRELLAQLEAHALDVVLANSAAPRDARSSLRNH
                     LLREQPVSLVGRPRTPRRRFRFPEDLKTERLLLPSLDSEIRVAFDRLLERTGVRPAIL
                     AEVDDMAMLRLLARERDGLTLVPPIVVRDELRAGTLVEYARIPEVSERFYAIVQKRRF
                     PNPMLARLLDLA"
     gene            1088..2665
                     /locus_tag="PL152_03185"
     CDS             1088..2665
                     /locus_tag="PL152_03185"
                     /inference="COORDINATES: protein motif:HMM:NF006029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HQR48310.1"
                     /translation="MLSEFTPFLVPLPLVTAAAAAFLAAGRRPRHSYWFAAAGALGAL
                     VAACVAAVALLLDGPATGSLAGGPLGWLSVRLDAVSAVMLLLVAFVGWVVLRYTRTYL
                     DGEARQGPFTGWLCLTLACVMLLVAAGNLFQLLAAWVATSLSLHRLLLFYPDRAAARR
                     AARKKYLVARTGDIALAGAVALLLGAYDTADIATILAAARAGEGGTVAGGAAALLAVA
                     AVLKSAQFPTHGWLTEVMETPTPVSALLHAGVINAGGLLLIRFADVMLLAPVVLGALV
                     VVGAGTALIGGLVMLTQPAVKTSLAWSTVAQMGFMILECGLGLFSLALLHIVAHSLYK
                     AHAFLASGGAVERVVALRRPGPAAAPGVGTVALAFAVAAAVYAVVTYALGSTAKSPQS
                     IALGAILVFGVADLLAQGWAGLGPHRLAPRMLAWAAATSLSYFLLQAAAVRLTAGTLP
                     AAPAPGPLEWLLMAATVLAFGAVATAQVLLPRWRAHPAAVRLRVHLSNGLYANAVFDR
                     LTGGWSTRRRLTTETER"
     gene            2668..5091
                     /locus_tag="PL152_03190"
     CDS             2668..5091
                     /locus_tag="PL152_03190"
                     /inference="COORDINATES: protein motif:HMM:NF021578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2309 domain-containing protein"
                     /protein_id="HQR48311.1"
                     /translation="MHKPSLDTMDARDPVRLATERAVRSIPPLWPLASSVAVNPFLGQ
                     AGDSLAAAGARLARVAGIRVTMPRSWYRERMEAGDISDADLQDALARSPALSHLPSVA
                     ALRHAAALEPPEPVALPTVADIAARVSGTDWPGLIAERIGAWAAGYFDEGQATWPAPL
                     ERGAYAEWRAVATCDLTPEIAGLREFTRHVAQAPADAAAAIERVAGRLGLTAEAMETY
                     FHQMLVTTGGWAQYARYRLWLAELAGQSDPTVRDLLAIRLLWEEALYLRYQPQVDGHW
                     ADVRARHASPVEPTPDLMVDTLLQDAAECAAQRRLAATLARGARATGDERPALQAAFC
                     IDVRSEVFRRALETVSPAVRTLGFAGFFGLPAAHRRYASDIEELRLPVLLNPALRSRA
                     ATAENPTDERSVRVAAQAHRAWERFKLAAVSCFAFVEAAGPLYVAKLLRDTLGLRTAP
                     APHDPPPQFDPPLQADRRADVAESVLRAMSLTTGYAPLVLLVGHGASVTNNAHASSLH
                     CGACGGYPGDVNARLLAAILNDPVVRAALVPRGIEIPPDTLFVAALHDTTTDRVTLYD
                     HDHPSAAHRAALAGARSWLDAAGRMARTERALRLPRADHEGTIARRSRDWSETRPEWA
                     LAGCMAFVAAPRARTAGTSLAGRAFLHDYDWTRDEQFKVLELVLTAPVVVASWISLQY
                     YGSTVAPAIFGAGNKLLHNVTGGIGVIEGNGGLLRAGLPWQSVHDGERWMHEPLRLTV
                     CIEAPVDAITGVLERHGAVRALFDNGWLHLRALDDAGRMAWRYAGGLQWVPTEPAEQP
                     RGGPLADAA"
     gene            5113..6018
                     /gene="cysD"
                     /locus_tag="PL152_03195"
     CDS             5113..6018
                     /gene="cysD"
                     /locus_tag="PL152_03195"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004781 - sulfate adenylyltransferase
                     (ATP) activity [Evidence IEA];
                     GO_process: GO:0019419 - sulfate reduction [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQR48312.1"
                     /translation="MRTSLTHLAALESEAIHIMREVVAEARNPVMLFSAGKDSTVMAL
                     LAVRAFHPARPPFPLLHVDSTWEFRSLIEFRDAFARRHGFELVAVANEEGRAAGINPF
                     DHGDRYTTIMRTETLKAALDRGGYDFILGGARRDEEKSRAKERVFSVRSSSHAWDPRQ
                     QRPELWRLYNARLGAGQTARVFPLSNWTELDVWSYALLHDIDLAPLYYAAPRPVVERD
                     GTLIVVDDAGRMRLRPGEAITTRSVRFRTLGCWPVTGAIESNACDLPTIVLETLRASS
                     SERQGRMVDREGGGSLEQKKREGYF"
     gene            complement(6038..7273)
                     /locus_tag="PL152_03200"
     CDS             complement(6038..7273)
                     /locus_tag="PL152_03200"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQR48313.1"
                     /translation="MNDTTLIDLAPADARANPIGGVARRLVLGRLSALECGRLHILER
                     GQAQDFQGTRPGQAAALEVRDPAFYAELAYGGSVGAAESYMLGHWTTPDLTALLRLML
                     ANRAALDALETGLARLSAPLRLAAHWLHRNTRSGSRRNISAHYDLGNEFFELMLDETM
                     MYSCALFERPDMSLADASRAKLDLVCRKLDLRPGHRVVEIGTGWGGFALHAASRHGCH
                     VTTTTISPSQYALARERVRAAGLEDRIEVLLEDYRDLRGRYDRLVSIEMIEAIGHRQF
                     EEFFRRSAGLLERDGRMVLQSITIADRHYARARDEVDFIKRYIFPGCCIPSVGALTEA
                     MVAGSDLRLVHLEDIGPHYATTLAAWRRNFHARLDAVRALGYPETFVRMWEYYLCYCE
                     AGFAERALGDVHMVLTREG"
     gene            complement(7270..8040)
                     /locus_tag="PL152_03205"
     CDS             complement(7270..8040)
                     /locus_tag="PL152_03205"
                     /inference="COORDINATES: protein motif:HMM:NF018770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HQR48314.1"
                     /translation="MHSALYTGRLSHRRRQPVPHAFDYAVCYAWLDLAELDAVFAGRW
                     FWSTRRPALAWLRRADYLGDPAAPLDAAVRDRVEAETGRRPTGPVRMLTLLRTFGHCF
                     NPVTFYYCYDPGGSRVETVIAEITNTPWGERHAYVLPAERGLRADGSLRFRFGKAFHV
                     SPFLPMDQGYDWTFTEPGAALGIRMVNHDGDERVFDATMYLERREIDGRALAWALVRH
                     PFAALNVLRRIYWQAFRLWLKRVPFHDHPATRTEEPAT"
     gene            complement(8009..>8743)
                     /locus_tag="PL152_03210"
     CDS             complement(8009..>8743)
                     /locus_tag="PL152_03210"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR48315.1"
                     /translation="FPARFFVRFLHNHGMLTVNDRPLWRTIRGGSARYVERLVAPFRD
                     RIHLGTPVEQVRRIPGGVLVKPRGHEAVRYDALFLACHSDQALALLADPTPDEREVLS
                     AIPYQANEAVLHTDARLLPRRRLAWAAWNYHVRPQGGPVALTYNMNILQQLDAPEPFL
                     VTLNRSDDIDPQRVLRRITYHHPLYTPASVAAQARHREIDGVRATYFCGAWLRNGFHE
                     DGVASALAAVRHFGEDHAQRALHRSA"
     assembly_gap    8744..8843
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8844..9143)
                     /locus_tag="PL152_03215"
     CDS             complement(<8844..9143)
                     /locus_tag="PL152_03215"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR48316.1"
                     /translation="MKVAVVGAGIAGNVAARELNREHEVTVFEAGQHVGGHTHTHAVE
                     AHGRTWNVDTGFIVYNDRTYPHFVALLDELGVASQASSMSFSVRDEAADVEYNGTS"
     gene            complement(9140..10075)
                     /locus_tag="PL152_03220"
     CDS             complement(9140..10075)
                     /locus_tag="PL152_03220"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016717 - oxidoreductase activity, acting
                     on paired donors, with oxidation of a pair of donors
                     resulting in the reduction of molecular oxygen to two
                     molecules of water [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA desaturase"
                     /protein_id="HQR48317.1"
                     /translation="MRSRLAVALRRWVDTAAAGGETRGGPRGIDWPRVVPFVLLHVGC
                     LGVVWTGWSPIAVAIAATLYALRMFAVTAFYHRYFSHRAFRAARGWQFVFAVLGASAV
                     QRGPLWWASHHRHHHLHSDRPDDAHSALQDGFLWSHMGWFLARENFATRHHLVRDLAR
                     YPELRWLDRYDVAVPAALFVALWVLGAWLEAAYPALGTGPWQLVVWGFCISTVVLFHS
                     TFVINSLAHRWGSRRFETRDESRNNPLLALVTFGEGWHNNHHRYPGSARQGFYWWEVD
                     LSWYGLRLLAALGVVRDLRPVPRSVLVTGRARGRP"
     gene            complement(10072..10638)
                     /locus_tag="PL152_03225"
     CDS             complement(10072..10638)
                     /locus_tag="PL152_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HQR48318.1"
                     /translation="MSHAKILAAVAVLAIPLAGCGSVPRPAVPLVAHVDVERFMGDWY
                     VIACIPTWIERDAWNAVESYRLEPDGTIATTFTFRQGGFAGPEKRYHPRGFIVDRASN
                     AIWGMQFVWPIKADYRIAYLADDYGETIIAREDRDYVWIMARTPTLDEAEYGRLTDRV
                     AAMGYDTAKLRKVPQQWPAPATASGGHP"
     gene            complement(10650..11294)
                     /locus_tag="PL152_03230"
     CDS             complement(10650..11294)
                     /locus_tag="PL152_03230"
                     /inference="COORDINATES: protein motif:HMM:NF040005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6134 family protein"
                     /protein_id="HQR48319.1"
                     /translation="MKAALAAALFALSGPALASGQGATLPVEREWNFRVLLDGREVGW
                     HRYVVRGEGESTEVESRAQFDVRFLFLNAYRYRHQAHERWRGACLDELESRTDTNGSV
                     EEVAAANHDGTLVVAGPSGDVRLPGCVMSFAYWDPRILGASRLLNSQTGELLPVRIDG
                     RGVERVAVGGRSVPATRHRLSAPDLQIDLWYADGRWVALEAPTPDGRLLRYELR"
     gene            complement(11291..12070)
                     /locus_tag="PL152_03235"
     CDS             complement(11291..12070)
                     /locus_tag="PL152_03235"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HQR48320.1"
                     /translation="MSVGSVWLASLPALAILAGAAWAVCTVRRNVGLVDIFWSLFFLA
                     AAVSYAVEAAPTGPRTTLLLVLVAAWALRLATHLAARNWNAPEDRRYQAIRTRNQPYF
                     EWKSLYLVFGLQGLLAWIISAPLAAAIASPAAFSVLDVAGALLATFGIAYEAVADAQL
                     ARFRRDPASAGRVLDRGLWRWSRHPNYFGEACVWWGFYVMAVAAGGAWTVFAPVLMTV
                     LLLRVSGVTLLERDIGERRPAYRDYVARTSAFVPWPPRSRA"
     gene            complement(12067..13122)
                     /locus_tag="PL152_03240"
     CDS             complement(12067..13122)
                     /locus_tag="PL152_03240"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQR48321.1"
                     /translation="MSQTLRAIGWIEQGLVPDAVVRAGIRRLCEQRLREIEAGDCERA
                     AATTEAFIAGMDGAELAPLPHLANEQHYELPARFFDLALGPHLKYSSAWWPDGTRTLE
                     EAEARALAATCARAGLVDGQDVLELGCGWGSLTLWVAANYRRSRITAVSNSASQRAWI
                     ESEARRRGLDNVAVLTADMNGFQAPGRYDRVVSVEMFEHMRNWRVLFGRVHDWLRPGG
                     RFFMHVFCHRSTPYAFVDGGPSDWMSRHFFSGGMMPSDDLALRFQDRLRLLARWRWDG
                     THYQRTANAWLENLDARRGEVLEVIAATYGDGAPQWLQRWRVFFMACAELFGYRNGQE
                     WWVSHYLFERPAAGGAA"
     gene            complement(13241..13885)
                     /locus_tag="PL152_03245"
     CDS             complement(13241..13885)
                     /locus_tag="PL152_03245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48322.1"
                     /translation="MKKISSKNLRNSIAGVALALSLGIAAPAHAAPSVQLTYGVTQVA
                     LSSDFVGALGSLGVTPDNILPGSLYVTSRGARAAFPIPTGELDANGPKLEILHSGGLT
                     LTAGGTRVALSSFIIENLGGALQLTGVVKVNNDIVGRIPLFNVALTEAPELTTSPSGK
                     LPGRSTKLSVEGANVTLTATAASALNGVFGVSAFTEGFPIGVAQINARVSDLDK"
     gene            complement(14038..15324)
                     /locus_tag="PL152_03250"
     CDS             complement(14038..15324)
                     /locus_tag="PL152_03250"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HQR48323.1"
                     /translation="MKPEGRIGARLTRTFALQGAFVSLAAVIGVFLAGVLLEGPLIRQ
                     ALRGEADYFWERRGARTDFPLPATRNLTGYLQDAPAAVAGMPSGFHPWRQGKTQYVVY
                     VSERAGQRLYLVFDRTNVDRLAIYYGLAPLAVLLLVLYLSTWLGYRATRRELSPVLAL
                     ARRVRQLDLAAPDPAAFAPESVAGRDDEIRELATALVRLTQRVKDLLERERNFTRDAS
                     HELRTPLTVIRMSAERLQQAPGLDEPGQRAVARIVRATVEMEDLTNALLLLARETETG
                     LPTELVSIRDVVAREMEKLQPIVQGRPVQLETEAHRDLLVEAPEKVIEVLLGNLLRNA
                     ASYTEQGSVRVKIDRDGVVVEDTGVGIAPDRLDELSQPFVRGASRRPGFGVGLAIVRR
                     ISDRFGWPVEFESEVGRGTRVRVRFPEATTPADGGA"
     gene            complement(15314..16015)
                     /locus_tag="PL152_03255"
     CDS             complement(15314..16015)
                     /locus_tag="PL152_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017356179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR48324.1"
                     /translation="MPQSPGSTLLLVEDNRDIAEAIVEFLELRGHAVDYAGDGVTGLH
                     LAVTNAYDAIILDVMLPGMDGIAVCRKLREEARRDTPILMLTARDTLEDKVAGLEAGA
                     DDYVVKPFEMRELEARIRALQRRQRGGVTRETLRVADLALDLGTLEVTRGGRVIRLMP
                     IGLRLLTALMRASPRVVSRQQLEREVWGEVPPDSDALRSHLYALRKSIDKPFDRALLH
                     TIPGVGYCIALRNEA"
BASE COUNT         2309 a         5672 c         5668 g         2276 t
ORIGIN      
        1 cgatcggcgg gccgcacctc ccgacttacg aatcgacgat gaggagcatc gacggcgcag
       61 cagccgcgcc gcagtagccg gcgtgccggt ggtgccaggc gcggttacgc gagatcgagg
      121 agccgtgcca gcatcgggtt cgggaagcgc cgcttctgca cgattgcata gaaccgttcg
      181 gacacttcgg gaatccgcgc gtactcgacc agggtgcccg cccgtagctc gtcgcggacc
      241 acgatcggtg gaaccagcgt caacccatcg cgctcgcgcg ccagcagccg cagcatcgcc
      301 atgtcgtcga cctccgccag gatggccggc cgcactccgg tccgctcgag gaggcggtcg
      361 aacgcgaccc ggatctcgct gtcgaggctc ggcagcagca gccgctccgt cttcaggtcc
      421 tcggggaagc ggaacctgcg ccgcggggtg cgtggacgcc cgaccaggct caccggctgc
      481 tccctcagca ggtggttgcg cagcgacgag cgcgcgtcgc gcggcgcggc gctgttcgcc
      541 aggaccacgt cgagcgcgtg cgcctcgagc tgcgcgagca gctcgcggat gctccccgag
      601 cggacgatca gctcgacgtc ggagcggccg accagtggcc gcaggaactc gagctggaag
      661 tttctcgaca gggtggtgag tgcgccgacg ctgagcacca cgcggttcgc cgctggcctg
      721 ccccgcagcg tggagagcag ttcgtccccg gcccggaaga ccgtatccgc gtagtcgagc
      781 gccatgcggc ccgcctcggt cagcaccagc ttgcggcccg cgcgctcgaa cagcgggtgc
      841 cccagctggt gctcgagctt cctgatctgc accgacaggg acgactgcga gacgtgcatg
      901 cgttcggccg cccgggtgag cccgcccgcg tgggccaccg cccagaagta gcgcaggtgg
      961 ttgaagttca cgggtcccat atcgttctta taaaacgaac gatatgcagg aaacaatgaa
     1021 tttttattgg cgcagggacg gccctagcat gccgccccgt ccggccgtgc gccgggggga
     1081 atcaccgatg ctcagcgagt tcaccccctt cctggtaccg cttccgctcg tgaccgcggc
     1141 tgcggccgcg ttcctggccg cagggcgtcg accgcggcac tcgtactggt tcgccgcggc
     1201 gggcgccctc ggggccctgg ttgcagcttg cgtggccgcc gtcgcgttgc tgctagacgg
     1261 cccggcgacg ggctcgctgg ccggcggtcc tctcggctgg ctgtccgtac gcctcgacgc
     1321 ggtgagcgcg gtgatgctgc tcctggtcgc cttcgtcggc tgggtggtgc tccgatacac
     1381 caggacgtac ctcgacggcg aggcgcggca ggggccattc accggctggc tctgcctgac
     1441 gctcgcctgc gtgatgctgc tggtcgcggc tggaaacctc tttcagctgc tggctgcgtg
     1501 ggtcgcgacc agcctgtccc tgcaccggct cctgctgttc tatccggatc gtgccgctgc
     1561 ccgccgcgcg gcccggaaga agtacctggt ggcacggacc ggggacattg cgctcgcggg
     1621 cgccgtggcg ctgctgctcg gcgcctacga taccgccgac attgcgacga tcctcgcggc
     1681 ggcgcgtgcc ggggaaggcg gcaccgtcgc gggtggcgcg gccgccctcc tcgcggtcgc
     1741 ggccgtgctc aagtcggccc agttcccgac ccacggctgg ctcacggagg tcatggagac
     1801 ccccacgccg gtgtccgcac tgctgcacgc cggcgtcatc aacgccggcg gactcctcct
     1861 catccggttc gccgacgtca tgctgctggc gcccgtcgtg ctgggggcac tcgttgtggt
     1921 cggcgccggc accgcgctga tcggtggcct cgtcatgctg acccagccgg cggtgaagac
     1981 ctcgctcgcg tggtccacgg tcgcgcagat gggattcatg atcctcgaat gcgggctcgg
     2041 gctgttttcg ctggcgctgc tgcacatcgt ggcccactcg ctctacaagg cgcacgcgtt
     2101 cctcgcgtcg ggcggggcgg tcgagcgcgt ggtggcactg cgcagaccgg gcccggccgc
     2161 ggcacccggc gtgggcacgg tcgcgctggc cttcgcggtc gcggccgcgg tctacgcggt
     2221 cgtgacgtat gccctcggct cgacggcgaa gtcgccgcag tccatcgcgc tcggtgcgat
     2281 cctggtcttc ggcgtggcgg acctgctcgc gcaaggttgg gccggcctcg ggccgcaccg
     2341 gctcgcgccg cgcatgctcg cgtgggcggc ggccacgtcg ctcagctact tcttgctgca
     2401 ggccgcagcc gtccggctca ccgccgggac cctgccggcc gctcccgcgc ccgggccact
     2461 cgaatggttg ctcatggcgg cgacggtcct cgccttcggc gcggtcgcaa ctgcgcaggt
     2521 gctgctgccg cgctggcgcg cgcacccggc cgccgtgcgc ctgcgcgtgc acctgtcgaa
     2581 cggcctgtat gccaatgccg tgttcgaccg gctcacgggc gggtggtcga cgcgacgccg
     2641 gctcacgact gaaacggagc gctgatcatg cacaagcctt cgctcgatac gatggacgct
     2701 cgcgatccgg tccgcctggc gacggagcgc gcggtccggt cgatccctcc gctgtggccg
     2761 ctcgcctcga gcgtggcggt caaccccttc ctcgggcagg cgggcgactc gctcgccgcc
     2821 gccggtgcgc gcctcgcacg cgtcgcaggg attcgggtca cgatgccccg cagctggtac
     2881 cgggagcgca tggaggccgg ggacatctca gacgccgatc tgcaggacgc actcgctcgc
     2941 tctccggccc tgtcgcactt gcccagcgtc gccgcacttc gtcacgccgc cgcgctcgag
     3001 ccgccggagc ccgtcgcgct gccgaccgtc gcggacatcg cggcgcgagt ctccggcacg
     3061 gactggcccg gcctgattgc ggagaggatc ggcgcctggg cagccggtta cttcgacgag
     3121 ggacaggcga cctggccagc cccgctggag cgcggcgcgt acgcagagtg gcgcgccgtg
     3181 gccacctgcg acctgacgcc ggagatcgcc gggctgcgcg aattcacgcg ccacgtggcg
     3241 caggcacccg cggacgccgc cgcagccatc gagcgggtcg cgggccggct cgggctgacc
     3301 gcggaggcca tggagacgta cttccaccag atgctggtga ccaccggcgg ctgggctcaa
     3361 tacgcacgct atcgactctg gctcgccgaa ctggcgggcc agagcgaccc gacagtgagg
     3421 gacctgctgg ccatccggct gctgtgggaa gaggctctct acctccgcta ccagccgcag
     3481 gtcgacggcc attgggccga cgtgcgcgcg cgccacgcgt cgccggtcga accgacgccc
     3541 gatctcatgg tcgataccct cctgcaggac gccgctgaat gcgcggcgca gcgccggctc
     3601 gccgcgaccc tggcgcgcgg tgcgcgcgcg acgggcgacg agcgccccgc cctgcaggcc
     3661 gcgttctgca tcgacgtgcg ctccgaggtg tttcgccgcg cgctcgagac agtcagcccg
     3721 gccgtgcgca cgctcggctt cgcgggcttc ttcggcttgc cggccgcgca ccggcgctac
     3781 gcgtcggaca tcgaggaact gcggctcccg gtgctgctca atcccgcgct gcgatcgcgt
     3841 gcggccaccg cggagaaccc cacggatgag cggtcggtgc gcgtcgcagc gcaggcgcat
     3901 cgcgcctggg agcgcttcaa gcttgcggcc gtgtcctgct tcgcattcgt cgaggccgcc
     3961 ggtccgctgt acgtcgcgaa actgctccgc gacacgctcg gactccgcac cgcgcccgcg
     4021 ccgcatgatc ccccgcccca gttcgatccc ccgctccagg cggaccgccg tgcagacgtg
     4081 gcggaatccg tgctgcgcgc gatgtcgctc accacgggct acgctccgct cgtgctgctc
     4141 gtcggccacg gcgccagcgt gaccaacaat gcccacgcca gctcgctgca ctgcggcgcc
     4201 tgcggcggct acccgggtga cgtcaacgcc cgcctgctcg cagcgatcct caacgacccc
     4261 gtggtccgcg ccgcgctcgt tccgcgcggc atcgagattc cgccggacac gctgttcgtg
     4321 gcggccctgc acgacacgac cacggaccgc gtaacgctct acgatcacga ccacccgtcg
     4381 gccgcccatc gcgcagccct tgccggggcc cgctcgtggc tcgacgccgc gggccgcatg
     4441 gcaaggaccg agcgagcgct gaggcttccc cgcgccgacc acgaagggac gattgcccgg
     4501 cgcagccgcg actggtccga gacccgcccc gagtgggcgc tcgcggggtg catggctttc
     4561 gtcgccgcgc cgcgagcgcg caccgcgggc accagcctcg ccggtcgcgc attcctgcac
     4621 gactacgact ggacgcggga cgagcagttc aaggtgctgg aactggtcct gaccgcgccg
     4681 gtggtcgtgg ccagctggat cagcctgcag tactacggct cgacggtggc gcccgcgatc
     4741 ttcggcgccg gcaacaagct gctgcacaac gtcaccggcg ggatcggcgt gatcgagggc
     4801 aacggcgggc tgctccgcgc cgggctgccc tggcagtcgg tgcacgacgg cgaacgctgg
     4861 atgcacgagc ccctgcgcct gacggtctgc atcgaggcgc cggtcgacgc gatcaccggc
     4921 gtgctggagc ggcacggtgc ggtccgggcg ctgttcgaca acggctggct gcacctgcgc
     4981 gccctggacg acgccgggcg catggcctgg cgctacgcag gcgggctgca gtgggtcccg
     5041 acggagcccg cggagcagcc gcgcggcggc ccgctcgcgg acgccgcctg accaccgcgg
     5101 caggcacacc tcgtgcgaac gagcctcacc cacctcgcgg cgctcgagtc cgaggcgatc
     5161 cacatcatgc gcgaggtggt cgccgaagcg cgcaaccccg tcatgctctt ctcggcgggc
     5221 aaggactcga cggtgatggc gctgctggcc gtgcgcgcgt tccatccggc acggcctccg
     5281 ttcccgctgc tgcacgtgga ctcgacctgg gagttccgtt cgctgatcga gttccgcgac
     5341 gcgtttgcgc gccgccacgg cttcgagctc gtcgccgtcg cgaacgagga gggccgggcc
     5401 gccggcatca accccttcga ccacggcgac cgctacacga cgatcatgcg caccgagacg
     5461 ctgaaggccg cactcgaccg gggtggatac gacttcatcc tcggaggcgc ccgtcgcgat
     5521 gaagagaagt cgcgcgccaa ggagcgcgtc ttctcggtcc gcagctcctc tcacgcctgg
     5581 gatccccgcc agcagcgccc ggaactctgg cggctctaca acgcccgcct gggcgccggg
     5641 cagacggcgc gcgtctttcc gctgtcgaac tggaccgaac tcgacgtctg gagctacgcc
     5701 ctgctccacg acatcgacct tgcgccgctg tactacgccg cgccacggcc ggtcgtcgag
     5761 cgcgacggca cgctgatcgt cgtcgacgac gcgggccgga tgcgcttgcg gcctggtgaa
     5821 gcgatcacca cgcggtcggt acgcttccgc acgctcggct gctggccggt gaccggggcg
     5881 atcgagtcca acgcatgcga cctgccgacg atcgtgctgg agaccctgcg ggcatcctcc
     5941 tccgagcggc agggccgcat ggtggatcgc gagggcggcg ggtcgctcga acagaagaag
     6001 cgcgaaggct acttctgacg cggcggcgtc cgcgctccta cccctcgcgc gtcagcacca
     6061 tgtgcacgtc gccgagcgcg cgctccgcga agccggcctc gcagtagcag aggtagtact
     6121 cccacatccg gacgaaggtc tccgggtagc cgagcgcccg caccgcgtcg agccgtgcgt
     6181 ggaagttgcg ccgccatgcg gcgagcgtgg ttgcgtagtg cgggccgatg tcctcgaggt
     6241 gcaccagccg gaggtcgctc cccgcgacca tcgcctcggt cagcgcaccg accgacggga
     6301 tgcagcagcc cgggaagatg tagcgcttga tgaagtccac ctcgtcgcgg gcacgcgcgt
     6361 agtggcggtc ggcgatcgtg atcgactgca gcaccatgcg accgtcgcgc tcgagcaggc
     6421 ctgccgagcg gcggaagaac tcctcgaact ggcggtgacc gatcgcctcg atcatctcga
     6481 tggacaccag ccggtcgtag cggccgcgca agtcgcggta gtcctcgagc agcacctcga
     6541 tccggtcttc gagcccggcg gcgcgcacgc gctcccgcgc gagcgcgtac tggctcggcg
     6601 agatcgtggt cgtcgtgacg tggcagccat ggcgcgacgc cgcgtgcagc gcgaagccgc
     6661 cccaccccgt gccgatctcg accacgcggt ggccgggccg caggtcgagc ttgcggcaca
     6721 cgaggtcgag cttggcacgc gaggcgtcgg ccagcgacat gtcgggccgc tcgaacagcg
     6781 cgcacgagta catcatggtc tcgtcgagca tcagttcgaa gaactcgttg ccgaggtcgt
     6841 agtgcgccga gatgttcctc cggctgcccg agcgggtgtt gcggtgcagc cagtgcgcgg
     6901 cgaggcgcag cggtgccgag agccgcgcaa gcccggtctc gagcgcgtcg agcgccgcgc
     6961 ggttggcgag catcagccgc agcagcgccg tgaggtcggg cgtggtccag tgcccgagca
     7021 tgtacgactc ggcggcgccg accgagccgc cgtaggcgag ctcggcgtag aaagcggggt
     7081 cgcgcacctc gagcgcggcc gcttgacccg gccgtgtgcc ttggaaatcc tgcgcctgac
     7141 cgcgctcgag gatgtgcagc cgtccgcatt cgagggccga gagccggccg aggacgagtc
     7201 gccgggccac gcctccgatc gggtttgccc gggcgtcggc gggtgcgagg tcgatcagcg
     7261 tggtgtcgtt catgtcgcgg gttcctcggt ccgggtggcc gggtggtcgt ggaaggggac
     7321 gcgcttcagc cacagccgga acgcctgcca gtagatgcgg cggagcacgt tcaaggcggc
     7381 gaaggggtgg cgcaccagcg cccaggcgag cgcacgaccg tcgatttcgc ggcgttcgag
     7441 gtacatcgtg gcatcgaaga cgcgctcgtc gccgtcgtgg ttcaccatcc ggatgcccag
     7501 tgccgcgccg ggctcggtga aggtccagtc gtagccctgg tccatcggca ggaacgggga
     7561 gacgtggaag gccttgccga accggaaacg caggctgccg tcggcgcgca ggccccgctc
     7621 ggcggggagc acgtaggcgt gccgttcgcc ccagggcgtg ttggtgatct cggcgatgac
     7681 cgtctcgacg cgcgaaccac cggggtcgta gcagtagtag aacgtcaccg ggttgaagca
     7741 gtggccgaac gtgcgcagca gcgtcagcat ccgcaccggc ccggtcggcc gccggcccgt
     7801 ctccgcctcg acccggtcgc gcacggccgc atcgagcggc gcggccgggt cgccgagata
     7861 gtcggcccgg cgcagccagg cgagcgcggg gcgtcgcgtc gaccagaacc agcggccggc
     7921 gaacaccgcg tcgagttccg cgaggtccag ccacgcatag cacaccgcgt agtcgaaagc
     7981 gtgcggcacc ggctggcgcc tgcggtggct aagccgaccg gtgtagagcg cgctgtgcat
     8041 ggtcttcacc gaagtgcctc acggcggcga gcgcgctggc gaccccatcc tcgtggaagc
     8101 cgttgcgcag ccacgcgccg cagaaatagg tcgcgcgcac gccgtcgatc tcgcggtggc
     8161 gcgcctgcgc cgccaccgac gccggcgtgt agagcggatg gtgataggtg atccttcgca
     8221 gcacgcgttg cggatcgatg tcgtcgctgc ggttcagcgt gaccaggaac ggctccggcg
     8281 catcgagctg ctgcaggatg ttcatgttgt aggtcagggc caccggacca ccctgcggcc
     8341 gcacgtggta gttccacgcg gcccaggcga gacggcgtcg cgggagcagc cgggcatcgg
     8401 tgtgcagcac cgcctcgttg gcctggtagg gaatcgccga cagcacctcg cgctcgtccg
     8461 gcgtcggatc ggcgagcagc gcgagcgcct ggtcgctgtg gcaggcgagg aacaacgcgt
     8521 cgtagcgcac ggcctcgtgg ccgcgcggct tgaccagcac tccacccggg atccgccgca
     8581 cctgctccac cggggtgccc aggtgaatcc ggtcgcggaa cggcgcaacc agccgctcga
     8641 cgtagcgcgc cgacccgccg cggatcgtgc gccacagcgg gcggtcgttc accgtcagca
     8701 tcccgtggtt gtgcaggaag cgcacgaaga accgcgccgg gaannnnnnn nnnnnnnnnn
     8761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8821 nnnnnnnnnn nnnnnnnnnn nnncgaggtg ccgttgtact cgacgtcggc ggcctcgtcg
     8881 cggaccgaga agctcatgct gctcgcctgc gaggcaacgc cgagctcgtc gagcagcgcg
     8941 acgaagtggg gatacgtccg gtcgttgtag acgatgaagc cggtgtccac gttccaggtg
     9001 cggccgtggg cctcgaccgc gtgggtgtgg gtgtgccccc cgacgtgttg gccagcctcg
     9061 aacacggtca cctcgtgctc gcggttgagc tcgcgtgccg cgacattgcc ggcgattccg
     9121 gcaccgacca cggcgacctt cacggccgcc cccgcgccct gccggtgacg agcaccgagc
     9181 gcggcacggg ccgcaggtcg cggaccacgc cgagcgcggc gagcagccgc aggccgtacc
     9241 agctcaggtc gacttcccac cagtagaaac cctggcgcgc cgagccgggg tagcgatggt
     9301 ggttgttgtg ccacccttcg ccgaaggtga ccagcgcaag caagggattg ttgcgcgatt
     9361 cgtcgcgggt ctcgaagcgg cggctccccc agcggtgcgc gagcgagttg attacgaacg
     9421 tcgagtggaa cagcacgacg gtcgagatgc agaatcccca cacgacgagc tgccagggcc
     9481 cggtgccgag cgccgggtac gcggcctcca gccacgcacc gagcacccac agcgcgacga
     9541 acaacgcggc cgggaccgcc acgtcgtagc gatccagcca gcgcagttcc gggtagcggg
     9601 ccaggtcgcg gaccaggtgg tggcgcgtcg cgaagttctc ccgcgcgagg aaccagccca
     9661 tgtggctcca caggaacccg tcctgcagcg cggagtgcgc gtcgtccggc cggtcggagt
     9721 gcaggtggtg gtggcgatgg tgggacgccc accacagggg tccacgctgc accgccgagg
     9781 cgccgagcac ggcaaagacg aactgccagc cgcgcgcggc acggaacgcg cggtgcgaga
     9841 agtagcggtg gtagaaggcc gtgaccgcga acatccgcag tgcgtaaagc gtcgcggcga
     9901 tggcgacggc aatcgggctc cacccggtcc acacgacgcc aaggcagcct acgtgcagca
     9961 gcacgaaagg aaccacgcgc ggccagtcga tgccgcgcgg accgccgcga gtctccccgc
    10021 ccgcagcggc cgtgtcgacc cagcgacgca gcgcgacggc gagccgcgac ctcacggatg
    10081 tcctccggac gcggtcgccg gggccggcca ttgctgcggt accttgcgca gtttcgcggt
    10141 gtcgtagccc atcgccgcga cccggtcggt gaggcggccg tactccgcct cgtcgagcgt
    10201 cggcgtgcgc gccatgatcc acacgtaatc gcggtcctcg cgggcaatga tggtctcgcc
    10261 gtagtcgtcc gccagatagg cgatccggta gtcggccttg atcggccaga cgaactgcat
    10321 gccccagatc gcattcgagg cgcggtcgac gatgaatccg cgcgggtggt agcgcttctc
    10381 cgggccggcg aagccgccct gccggaacgt gaaggtggtg gcgatggtgc cgtcgggttc
    10441 gagccggtac gactcgacgg cgttccacgc gtcccgctcg atccaggtgg ggatgcacgc
    10501 gatcacgtac cagtcgccca tgaatcgctc gacgtcgacg tgggcgacca gcggcaccgc
    10561 ggggcgcggc acgctgccgc aaccggcgag cgggatggcc aggaccgcga ccgcggcgag
    10621 gatctttgca tgtgacatcg gttgcaccct caacggagtt cgtagcgcag caggcggccg
    10681 tccggcgtgg gagcctcgag ggccacccag cggccgtccg cgtaccagag atcgatctgc
    10741 aggtcgggcg ccgagagccg gtggcgggtg gccggcacac tgcggcctcc gacagcgacg
    10801 cgctcgacgc cgcgcccgtc gatgcgcaca ggcagcagtt cgccggtctg cgagttcagc
    10861 agccggcttg cgcccaggat gcgcgggtcc cagtaggcga agctcatcac gcacccgggc
    10921 aggcgcacgt cgccggacgg accggccacg accagcgtgc cgtcgtggtt ggccgcggcc
    10981 acttcctcga ccgaaccgtt cgtgtcggtc cgcgactcga gctcgtccag gcacgcgccc
    11041 cgccagcgct cgtgcgcttg gtgccggtag cgataggcgt tcaggaacag gaagcggaca
    11101 tcgaactgcg cgcggctttc gacctcggtg gactccccct ccccgcgcac gacgtagcgg
    11161 tgccagccga cctcgcggcc gtcgagcagc acgcggaagt tccactcgcg ctccacgggc
    11221 agcgtcgctc cctggccgga ggcaagcgcc ggtcccgaca gcgcgaacag tgcagcggca
    11281 agcgcggcct tcatgcacga ctccggggcg gccagggcac gaacgcgctg gtgcgcgcca
    11341 cgtagtcgcg gtaggcgggc cggcgctcgc cgatgtcccg ctcgagcagc gtgacgccgg
    11401 agactcgcag cagcagcacg gtcatcagta ccggagcgaa cacggtccag gcgccgccgg
    11461 cggccaccgc catcacgtag aaaccccacc acacgcaggc ttcgccgaag tagttcgggt
    11521 gccgcgacca gcgccacaac ccccggtcga gcacgcggcc ggcgctcgcc gggtcgcgac
    11581 ggaatcgtgc gagctgcgcg tcggcgacgg cctcgtaggc gatgccgaac gtggcgagca
    11641 gcgcgccggc gacatcgagc acgctgaacg cggccggcga ggcgatggcc gcggcgagcg
    11701 gcgccgagat gatccaggcg agcagcccct gcaggccgaa caccaggtac aggctcttcc
    11761 actcgaagta cggctggttg cgggtgcgaa tggcctggta acggcggtct tccggcgcgt
    11821 tccagttgcg cgcggcgagg tgggtggcaa gccgcagggc ccacgcggcc accagcacga
    11881 gcagcaacgt cgtgcggggc ccggtcggcg ccgcctccac cgcgtaactc acggcggcgg
    11941 ccaggaagaa cagcgaccag aagatgtcca ccaacccgac gttgcggcgc acggtgcaca
    12001 ccgcccacgc cgcgccggcg aggatggcaa gcgcgggcag cgaggcgagc cagaccgacc
    12061 cgacgctcat gccgcccctc ccgccgccgg ccgctcgaac aggtagtggc tcacccacca
    12121 ctcctgaccg ttgcggtagc cgaacaactc ggcgcacgcc atgaagaaca cccgccagcg
    12181 ttgcagccac tgcggcgcgc cgtccccgta ggtcgcggcg atgacctcga gcacctcgcc
    12241 gcggcgcgcg tcaaggttct cgagccacgc gttcgcggtt cgctgatagt gggtaccgtc
    12301 ccagcgccag cgtgcgagca gccgcagcct gtcctggaag cgcagcgcca ggtcgtcgct
    12361 cggcatcatg ccgccggaga agaagtgccg gctcatccag tccgaggggc cgccgtcgac
    12421 gaaggcgtag ggcgtcgagc ggtgacagaa cacgtgcatg aagaaccgtc cgccgggccg
    12481 cagccagtcg tggacgcggc cgaacagcac gcgccagttg cgcatgtgct cgaacatctc
    12541 cacggaaacg acccgatcgt agcggccggg tgcctggaac ccgttcatgt ccgccgtgag
    12601 cacggcgacg ttgtcgaggc cgcggcggcg cgcctcggac tcgatccagg cccgctggga
    12661 ggcggaattc gacaccgcgg tgatgcggct tcgccggtag ttcgccgcca cccacagcgt
    12721 gagcgagccc catccgcagc cgagctcgag cacgtcctgt ccgtcgacga gtccggcgcg
    12781 cgcgcaggtc gcagccagcg cgcgtgcctc ggcctcctcg agcgtgcgcg tgccgtccgg
    12841 ccaccaggca ctggagtact tgaggtgcgg gccgagcgcg aggtcgaaga agcgggccgg
    12901 cagctcgtag tgctgctcgt tggcgaggtg aggcagcggc gcgagctccg cgccgtccat
    12961 cccggcgatg aatgcctcgg tggtcgccgc cgcgcgctcg cagtccccgg cctcgatctc
    13021 gcgcaggcgc tgctcgcaga ggcggcggat cccggcgcgc acgacggcgt cgggcacgag
    13081 gccctgctcg atccatccga tcgcgcggag cgtctgactc atgttgcttt ctccttcgcg
    13141 aaacaaggct gcccaacgtg cgcgtgcttg ctgagcccgt gggaggaagg ccccggttgc
    13201 ccggggcctt ccggtatcgc cgcgtgacgg gcgggttcgc tcacttgtcc aggtcgctca
    13261 cgcgcgcgtt gatctgcgcc acgccgatcg ggaagccctc ggtgaaggcg ctgacgccga
    13321 acacgccgtt cagggccgag gccgccgtcg cggtcagcgt cacgttggcg ccttccacgc
    13381 tgagcttggt cgaacggccg ggcagcttgc cggatggcga ggtcgtgagc tccggagcct
    13441 ccgtcagggc gacgttgaac agcgggatgc gcccgacgat gtcgttgttc accttcacga
    13501 ctccggtgag ctgcagcgcg ccgccgaggt tctcgatgat gaatgacgag agcgcaaccc
    13561 gggtgccgcc ggccgtgagc gtcagcccgc cggagtgcag gatctcgagc ttcggcccgt
    13621 tcgcgtcgag ttccccggtg ggaatcggga aggccgcgcg cgcccctcgc gaagtcacgt
    13681 acaggctgcc gggcaggatg ttgtccggcg tgacgccgag cgaacccagc gcgcccacga
    13741 agtcggacga gagcgccacc tgcgtaacgc cgtacgtgag ctgcacggac ggcgcggcgt
    13801 gcgccggagc cgcgatgccc aggctcagag cgagcgcgac gccggcgatg ctgttcctca
    13861 ggttcttcga gctgatcttc ttcatgacgg ttcctcctgg tgatgaccac gttccgggtt
    13921 gggcggcacc tgccgcgaat ctgcgtgggg gagtttccgt gccgcggcgt caaggccgcg
    13981 tgaaggcgcc gtgcagacgg cgtgaagggc cagcgcaggg gcaggcgggc ccggcgccta
    14041 ggcgccgccg tcggccggcg tcgtcgcctc cgggaatcgg acccggacgc gggtgccgcg
    14101 gccgacctcg ctctcgaact cgaccggcca gccgaagcgg tcggatatcc ggcgcacgat
    14161 cgccagcccg acgccgaatc ccggccggcg acttgcgccg cgcacgaacg gctgggacag
    14221 ctcgtcgagc cggtcgggcg caatgccgac gccggtatcc tcgacgacca cgccgtcgcg
    14281 gtcgatcttc acgcggaccg aaccctgctc ggtgtacgag gccgcgttgc gcagcaggtt
    14341 cccgagcagg acctcgatca ccttctccgg cgcctcgacg agcaggtcgc ggtgcgcctc
    14401 ggtctcgagc tggaccggcc gaccctggac gatgggctgc agcttctcca tttcgcgcgc
    14461 gacgacgtcc ctgatgctga ccagctcggt cggcaggccc gtttcggtct cgcgcgcgag
    14521 caggagcagc gcattggtca ggtcctccat ctccaccgtc gcgcgcacga tgcgcgccac
    14581 ggcgcgctga ccgggctcgt cgaggcccgg cgcctgctgc agccgctcgg ccgacatccg
    14641 gatcacggtg agcggagtgc gcaactcgtg gctcgcgtca cgagtgaaat tgcgctcgcg
    14701 ctcgaggagg tccttcacgc gttgcgtgag ccgcaccagc gcggtggcga gttcgcggat
    14761 ctcgtcgtcg cgccccgcca cgctttccgg cgcgaaagcc gccgggtccg gcgccgcgag
    14821 gtcgagctgg cgcacccgcc gggctagcgc gaggaccgga gacagctcgc gccgggtggc
    14881 gcggtagccg agccacgtcg acaggtacag gacgagcagc aggaccgcga gcggcgcgag
    14941 cccgtagtag atcgcgagcc ggtcgacgtt ggtgcggtcg aataccaggt agagccgctg
    15001 ccctgcccgc tcgctgacgt agacgacgta ctgggtcttt ccctgccgcc acgggtggaa
    15061 tcccgacggc atgccggcca cggccgccgg agcgtcctgc aggtaccccg tgagattgcg
    15121 ggtcgccggc agcggaaagt ccgtgcgcgc gccacgccgc tcccagaagt aatcagcttc
    15181 gccccgcagc gcctgcctga tcagcgggcc ttcgagcagc acgccggcga ggaacacgcc
    15241 gatcacggcc gccaggctga cgaacgcgcc ctgcagcgcg aaggtgcgcg tcagccgcgc
    15301 gccgatcctg ccctcaggct tcattgcgca gcgcgatgca gtagccgacg ccgggaatcg
    15361 tgtgcaggag cgcacggtcg aacggcttgt cgatggactt gcgcagtgca tacaggtggc
    15421 tgcgcagcgc gtcggaatcc ggcgggacct ctccccagac ctcgcgctcg agctgctgcc
    15481 ggctcacgac ccggggcgac gcgcgcatga gcgccgtgag caggcgcagc ccgatgggca
    15541 tcagccggat cacgcgcccg ccgcgcgtga cctcgagcgt accgaggtcg agcgcgaggt
    15601 ccgcgacacg cagcgtctcg cgggtcactc cgccgcgctg ccggcgctgc agggcgcgga
    15661 tgcgcgcctc gagctcgcgc atctcgaaag gcttcacgac gtagtcgtcg gcaccggcct
    15721 cgagccccgc caccttgtcc tcgagcgtgt ccctggccgt gagcatcagg atcggcgtgt
    15781 cgcggcgcgc ttcctcgcgc agcttgcggc agacggcgat gccgtccatc ccgggcagca
    15841 tcacgtcgag gatgatcgcg tcgtaggcgt tggtcaccgc caggtgcagg cccgtgacgc
    15901 catcgcccgc gtagtccacg gcgtggccgc ggagctcgag gaactccacg atcgcttccg
    15961 cgatgtcccg gttgtcctcg acgagcagca gcgttgagcc agggctctgc ggcatgagtg
    16021 gatcg
//
LOCUS       DAOMFD010000030        15978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_776_length_15978_cov_36.715694, whole genome shotgun sequence.
ACCESSION   DAOMFD010000030 DAOMFD010000000
VERSION     DAOMFD010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15978
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_776_length_15978_cov_36.715694"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2463
                     /locus_tag="PL152_03260"
     CDS             <1..2463
                     /locus_tag="PL152_03260"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HQR48325.1"
                     /translation="ALGDWGDAAFIGIVLAVNSALGGWQEWRAEQQSRSLQQLLKIRA
                     TVLRDGVIVEIDADQLVPGDIVAIESGQRVPADLLLLAARGLEVDEALLTGESVPVSK
                     QPGRAGPGESGSSAGDRRDMAWAGTTIVRGRAEGEVVATGAATAVGNLATVMSATEGG
                     KPPLVGRMEHFARVIAVVVLVAAVVVAVVGVVARGESIATMFLFGVALAVSAIPEGLP
                     VALTVTLAIAARRMASRGAIVRSLPAVEGLGSCTLIASDKTGTLTCNELTVRELRLAD
                     GSVMQVTGAGYEPVGEILPVTGGEAAGSAQCVRRLLEIAAACNEASLVRQDGRWTSRG
                     DPTDVAFLALAAKGGISRERLRVERPVAADIPFEPERRYAATFHASDDATWIAVKGAP
                     ERVVGMCRLDDRASRDVLEQAERMAERGLRVMALASGTAPGAPHAGEVPAEPAGLEMA
                     GLAGLIDPLRPGAREAVERCAGAGIRVIMVTGDHPVTALAIARELGAAVGRDEVITGD
                     AFRSAGAAERDAMVAHATVFARVTPEQKLAIVEAAQRDGHYVAVTGDGVNDAPALRRA
                     NIGVAMGRGGTDVARDASDLVLSDDNFATIVTGVEQGRIAYQNIRNVVYLLIAAGTAE
                     VLTVALAVFAGLPLPLLPVQLLWLNLVTNGIQDVALAFERGHGDELRAPPRRPGEPLV
                     NRLMVERGLLAGLWMSAIGFAWFAALVHSGTPVAHARNELLLLMVLMQNVDAFNARSE
                     TRPVFTLPLASNPLLAAGVATALALHVSAMYLPPLQRLLDVAPLTIDEWIEMPLVALT
                     LLVVMEIHKRGWAVRARAGATN"
     gene            2503..3387
                     /locus_tag="PL152_03265"
     CDS             2503..3387
                     /locus_tag="PL152_03265"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Dyp-type peroxidase"
                     /protein_id="HQR48326.1"
                     /translation="MSPIQPGILAPIPRVARHLSFTLAPDAGPGPALERLAQAVDPAA
                     TVVGLGRSISLALGRHVPGLRDFPSGAGRGFEIPATPGALWCWLRGEDRGELVHRSRA
                     LQALLAPAFALDSVVDAFMHEDGRDLTGYEDGTENPTGERAVATASLQGAAAGLDGSS
                     YAAVQQWVHDFAAFEALGPDEQDLSVGRRRADNEEIDEAPPSAHVKRTAQESFSPEAF
                     VLRRSMPWAEGAKAGLVFLAFGRSFDAFEAQLSRMTGLDDGITDALFRFTRPVTGAYY
                     WCPPVSGGRLDLRALELR"
     gene            complement(3422..5062)
                     /locus_tag="PL152_03270"
     CDS             complement(3422..5062)
                     /locus_tag="PL152_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR48327.1"
                     /translation="MQTPRRNLTPSTLRLGRRYRPNRVEGRYDALVIGSGIGGLTTAA
                     LLSEIGWRVAVLEQHYTAGGATHSYDRAGYEWDVGVHYIGDMGARTTSRRMMDFLTQG
                     NLHWAPMDSHYDRFFIGERTYDAVAGRDAYRDNLVGYFPREAAAIDRYLELLGETARG
                     MRTFSLDRTLPPWAAAIAGPFLRSRLPRSFGRTTWEVLSELTRDAELIAVLTGQWGDL
                     GLPPKRSAWVIQALVAKHYLHGGFYPVGGAWRIADTILPRIRAAGGEVFTYARVQEVL
                     LEPARRHAVRGVRMEDGSEIACDCVISDAGALNTFGHLVPEETRRRVGYDRLLARVKP
                     SIGHLCVYIGLKGRAEDLGLPKTNYWIYHDNDYDAAYDRFAADPHGPFPAVYISFPSA
                     KDPDFERRHPGRSTIEIVAPAPYSIFEPWAGTTWGKRGDDYEALKQAFGERLLEHLYA
                     KLPALRDRIDYWEISTPLSMQWFCGYARGELYGLDHDPERMRQSWLRPRTRIPGLWLT
                     GQDVMSCGVTGAMMGGLASAVSIGGTRRMAPVLKGIFG"
     gene            5302..5781
                     /locus_tag="PL152_03275"
     CDS             5302..5781
                     /locus_tag="PL152_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpW family outer membrane protein"
                     /protein_id="HQR48328.1"
                     /translation="MLAGLYACAAASLAVAGDDGPDWFRRSAQPLTNLDMESADEFLV
                     VLQRDRVDGPDLLTLRYTLGQVGPLSTYAGAGINRTSYFAWSVNPDDGLLDRSRHRAI
                     GAAAELGAAWRVSNTLALAADLHWIDLASDADMLRVGSTMVAADAVAVGVSLGWRFR"
     gene            complement(5790..8105)
                     /locus_tag="PL152_03280"
     CDS             complement(5790..8105)
                     /locus_tag="PL152_03280"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HQR48329.1"
                     /translation="MGKTIALATPVFFALIAIEYLVGRARGRHNYRLNDAVNSLSLGI
                     MSQVVGLFTKAAAVGIYVWTYESFAVWRLPAEQWWVWVAGLVVYDFCYYWNHRLGHVS
                     AVFWASHVVHHQSQEYNLTTALRQTSSGALLGWIFYLPMAIAGFPPQVFVVAAIIDLL
                     YQYWIHTEQVGKLGWFDRWFASPSNHRVHHAVNDRYIDRNYGGIFMAWDRMFGSFVEE
                     TEPCVYGTRASLDSWDPLWANLEVYVDLARKSWSAQRWRDRLLVWIKPPGWHPATETG
                     PAWDKPPFALDKLRRYDPPMTSAVRCYVALHFVAVLLAATTLLWRSSALPFAQVAAWS
                     AGVLAVLWLTGTVMQRRLRVPTALAIEFVALALLAGATAVRAAPVTIPTIVDDAAVQQ
                     AVETARSEFLATQDFDRLQVTVLVEDKDHGWLRGSVGGDELAYPASCVKLPFLVGAVH
                     WCAAQGKRPDCLDEWVSPMILRSSNVATGWVVDTVTGAPNGPPDGADLGAFIERRRYT
                     ERVLDRAGLLGNQRFFTKTYPTNSGSEPSGLEELAWKEMGRNMMSSNGAANLMLAVAT
                     GAIEQQATAYMRGLLRRPTFSDQASLGGGLPPGSLQENKVGAAFDTLEDVMYAELPNG
                     RRIVVAALSNGWNPLEPQPWDVVRLGSLTERVLARLGIAEGLPAPRYLEPARLPDGAF
                     TANLDVPADGRYEIAVWYSADPRHTPAALYEVDDADEGLRLELDQRVWGARWIKLGDF
                     DLRRGTGTVRVSSTAPGTLDGGRLRITRWAR"
     gene            complement(8116..9609)
                     /gene="glpK"
                     /locus_tag="PL152_03285"
     CDS             complement(8116..9609)
                     /gene="glpK"
                     /locus_tag="PL152_03285"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HQR48330.1"
                     /translation="MRRFVLALDQGTTSSRSILFDHDGQVVATAQREFTQHFPRSGWV
                     EHDAEEIWATQAATIAEVLARARATPSDLAAVGITNQRETTVLWDRRSGRPVAPAIVW
                     QDRRTAELCARLKSDGHEPEIARRTGLLLDPYFSGTKLKWLLDSDPALRKRATAGELA
                     FGTVDSWLAWKLSQGAVHVTDASNASRTLLLDLATGTWDDYALELLRIPRRVLPRVVP
                     SRLPDGDAVAVIGGEEVPLGGIAGDQQAALFGQGCFAPGMAKNTYGTGCFMLMNTGLE
                     PLHSANRLLTTIAWDTGERHYALEGSVFVGGAVVQWLRDGLGLIERSADVEALAASVP
                     DTGGVYLVPAFTGLGSPHWDAYARGLVVGLSRGTTRAHLARAALEAIAFQSAEVLAAM
                     QKDARQPLTELRVDGGATANDLLMQFQADLLGVPVVRPRVTETTALGAASLAGLSVGF
                     WQSTDEVAANWRADRRFEPAMSRESAAARMRQWSRAVERSRGWHDEP"
     gene            complement(9659..11185)
                     /gene="glpD"
                     /locus_tag="PL152_03290"
     CDS             complement(9659..11185)
                     /gene="glpD"
                     /locus_tag="PL152_03290"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF008899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HQR48331.1"
                     /translation="MSGGARQYDLLVVGGGINGVGVARDAAGRGLGTLLCEQHDLGAH
                     TSSASTKLIHGGLRYLEFYDFRLVRKSLREREIVLRSAPHLVRPMRFVLPHDEHLRPA
                     WMIRAGLYLYDHLARRRLLPASRAVDLRRHPAGAALDPRYTQGFVYSDAAVDDARLVV
                     LAALDARERGATVLSRTRCVRLERSGDRWTATLAHADGAESRISAAAVVNATGPWVDR
                     FLDEATPVPARRHPRLVKGSHIVVPRLFEHDHAYIFQGPDRRIVFAIPYEGDCTLVGT
                     TERDYTGDLATPSIEPAEVTYLLSMSNRYFRRMVGHADVKWTFAGLRPLLADPSDVAT
                     SVTRDYVLDVDQQGPPLLSIYGGKLTTYRKLAEDVLDGLASRMGWSRRHWTAGAPLPG
                     GDIARADYAAFRRSVRAEYPWLPDGLLDRMAAAYGTRVRRMLGDARGISALGEEVLPG
                     LHAREIDYLRREEWALTAEDILYRRSKLALHLPADAAERLEEWLVRNPVARPADYAAG
                     "
     gene            complement(11198..12403)
                     /locus_tag="PL152_03295"
     CDS             complement(11198..12403)
                     /locus_tag="PL152_03295"
                     /inference="COORDINATES: protein motif:HMM:NF018743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HQR48332.1"
                     /translation="MRFGIDRLLAEPDQRRPLAGRRVALLAHPASVTADLTHSLDAIA
                     ALGDIRLAAAFGPQHGLRGDKQDNMVESPDFRDARHGIPVFSLYGEVRRPTPAMLDTF
                     DVLLVDLQDLGCRIYTFVTTLRYVLEAAAQHRKSVWVLDRPNPIGRPVEGLTLRQGWE
                     SFVGAGEMPMRHGLTLGEMGHWFVRRLRLDVDYRVIAMQGWDPSRGPGYGWPLGERVW
                     VNPSPNAPNPWMARCYPGTVMLEGTTLSEGRGTTRPLELFGAPGLDIPAIVAEMRRLA
                     PAWLAGCRLRACWFEPTFHKHAGQLCAGLHYHVEDGSYGHESFRPWRLQALAFKALRR
                     LHPEFELWRHFPYEYEHDRLAIDLINGSDLLRRWVDDPAAASADLDALAVADEEAWLD
                     QRSSAMIYG"
     gene            complement(12400..12909)
                     /locus_tag="PL152_03300"
     CDS             complement(12400..12909)
                     /locus_tag="PL152_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DOMON-like domain-containing protein"
                     /protein_id="HQR48333.1"
                     /translation="MQRIDVELESTGDGDLRLRYFLDGDVAAITLPPHAAPRQADGLW
                     QHTCFEAFIAGQGSRAYCEFNFSPSTEWAAYGFSAYRAGMVPLQYSTPPTVSVSVAED
                     RIALEAIIPLEALLALPGDGMLLVSLAAVVEDRDGRLSYWALAHRAEKPDFHSPAGFV
                     LEIERATSW"
     gene            complement(13008..14084)
                     /locus_tag="PL152_03305"
     CDS             complement(13008..14084)
                     /locus_tag="PL152_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48334.1"
                     /translation="MNRREAVIELLAGPGAAEHVRANGVVPADVACIPAAAGGPKGLA
                     LIPLDQLLYREWLKDTAPIELVGASVGAWRMAALAQPDPVAALERLRYSYVHDQNYDR
                     KPTPDDVARACRRLARAVLGDAPLRLRPGVTLAIVTSRARGALEGTESTLAFGRAGLA
                     NAISRRSLAAHLERVVFSTGRTFLASPFDEFGSRVVALDPANTEDALVASGTIPIVCA
                     PVRGIAGAPPGNYWDGALVDYHLQLPYERLTRSGPRRRIVLYPHFNDYVTPGWLDKHL
                     PWRRKSRGHPWLDDMLLVAPSRSFLASLPNGKLPDRKDFYRYGQDHAGRIRDWDRAIR
                     ECQRLADAFLHWLERPDPTVLRAL"
     gene            complement(14090..15361)
                     /locus_tag="PL152_03310"
     CDS             complement(14090..15361)
                     /locus_tag="PL152_03310"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR48335.1"
                     /translation="MQHVASYYAATAHPAPPRPALAGQVRADVCVVGGGIAGCSAALD
                     LASRGYRVVLLEARRVGWGASGRSGGQAIFGFGASQDVVVDQVGPEAARLMWDVSVEA
                     LALLRHRVEEHRIDCDLHWGHLHVATKERQRRELEALHAELGERYGYAGTRMLDRPDV
                     EALLATRRYCAGLYDPGSGHLHPLNYTLGLARAAETAGARLFEASRVRSVRHGGTVRL
                     STSAGHVDADHVIFARGGYLDGVRTVADWRVMPVGTYVVSTQPLGEARIRELVRENVA
                     VADVNFVLDYFRCSADHRLLFGGRVSYSGIDARNTGHATRARMLRVFPQLADARLDYV
                     WGGYVDITMNRVPDFGRIAPNVLYLQGFSGHGIAMAGMAGRLAADAVAGQAERFDLFG
                     HVPHRVFPGGRALRTPALVLGMLWYRLRDLL"
     gene            15406..>15978
                     /locus_tag="PL152_03315"
     CDS             15406..>15978
                     /locus_tag="PL152_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48336.1"
                     /translation="MARIAASGKLGSMQDFDLHAPWPSQADAVPVSKRGRDLIRDPLL
                     NKGTAFRADERERFRLHGLLPSRQISMDLQVQRNVASIDRAATPLDQYLRLSALQDRN
                     EHLYHRVLAENLDRFMPIVYTPTVAEATRNFSQVFRRARGVWLTPDFRGRMARVLSDA
                     AGDEPVRLIVATDNESILGIGDQGAGGMAIC"
BASE COUNT         2381 a         5574 c         5659 g         2364 t
ORIGIN      
        1 gcgctgggcg actggggcga cgccgcgttc atcggcatcg tgctcgccgt gaactcggca
       61 ctcgggggct ggcaggaatg gcgcgcggag cagcagagcc gcagcctgca gcagctgctc
      121 aagattcgcg cgaccgtcct gcgcgacggc gtcatcgtcg agatcgacgc ggaccagctc
      181 gtgccgggcg acatcgtggc gatcgagagc ggccagcgcg tgcccgcgga cctgctcctg
      241 cttgcggcgc ggggcctgga agtggatgag gcgttgctca ccggcgagtc ggttcccgtg
      301 tccaagcagc cgggccgcgc aggcccaggc gaaagcgggt cgtcggcggg cgaccgccgc
      361 gacatggcgt gggccggcac gacgatcgtg cgcgggcggg cggagggcga ggtcgtggcg
      421 accggcgccg cgacggccgt cggcaacctc gcgaccgtga tgtccgcgac cgagggtggc
      481 aagccgcccc tggtcggtcg catggagcac ttcgcccgcg tcattgccgt cgtcgtgctc
      541 gtcgcggccg tcgtcgtggc agtggtcggc gtggtcgccc gcggggaaag catcgcgacg
      601 atgttcctgt tcggcgtcgc gctcgccgtc tcggcgatcc cggagggcct gccggtcgcg
      661 ctcaccgtga cgctcgcgat cgccgcgcgc cgcatggcca gccgtggtgc gatcgtgcgt
      721 agcctgccgg ccgtcgaggg cctcgggagt tgcacgctga tcgcgagtga caagaccggc
      781 acgctcacgt gcaacgaact caccgtgcgc gagctgcgcc tggccgacgg cagtgtcatg
      841 caggtcaccg gcgcgggtta cgagcccgtg ggtgagatcc tgccggtgac aggtggcgag
      901 gcggccggca gcgcgcagtg tgtgcgccgg ctgctggaaa tcgccgccgc ctgcaacgag
      961 gcgagtctcg tgcgccagga cggtcgctgg acgagccggg gggacccgac cgacgtcgcg
     1021 ttcctggccc ttgcggccaa gggagggatc agtcgggagc gactgcgcgt cgagcgcccc
     1081 gtcgccgccg acatcccgtt cgaaccggag cggcgctacg cggcaacgtt ccatgcgagc
     1141 gacgacgcga cctggatcgc agtcaagggt gcgccggagc gagtggtcgg catgtgccgg
     1201 ctcgacgatc gtgcgtcccg cgacgtcctc gaacaggccg agcgcatggc ggagcgcgga
     1261 ttgcgcgtga tggcgctggc atcggggacc gctcctggtg cgccgcacgc aggagaagtt
     1321 ccggccgagc cagcaggcct ggaaatggcg ggtctggctg gcctcatcga tccgctgcgg
     1381 cccggcgctc gcgaagccgt cgagcgctgc gcaggcgccg gcatccgggt catcatggtg
     1441 acgggcgacc acccggtgac tgcactcgcg attgcgcgcg agctcggcgc tgccgtcggc
     1501 cgcgatgaag tcatcaccgg ggacgcattc cggtccgccg gcgccgccga gcgagacgcg
     1561 atggtcgcgc atgcgaccgt cttcgcgcgc gtgacgccgg aacagaagct cgccatcgtc
     1621 gaggccgcgc agcgtgacgg gcactacgtc gcggtcaccg gcgacggcgt caacgacgcg
     1681 cccgcactgc gcagggccaa catcggcgtc gcgatggggc gcggcggcac ggatgtcgcg
     1741 cgcgatgcgt cggacctcgt gctgagcgac gacaacttcg cgaccatcgt caccggcgtg
     1801 gagcagggga ggatcgccta ccagaacatc cgcaacgtcg tgtacctgct gatcgcggcc
     1861 ggcaccgccg aggtcctgac ggtcgcgttg gccgtcttcg ccggcctgcc attgccgctg
     1921 ctcccggtgc agctcctgtg gctcaacctc gtcaccaacg gcatccagga cgtggcgctg
     1981 gccttcgagc gaggccacgg cgacgagctc agggcgccgc ctcggcgacc cggcgagccg
     2041 ctggtgaacc ggttgatggt cgagcgcggc ctgctggcgg ggctgtggat gtcggcgatc
     2101 ggatttgcgt ggttcgcggc tctcgtgcat tcgggcacgc cggtcgcgca tgcacgcaac
     2161 gagctgctgc tgctcatggt cctgatgcag aacgtggacg cgttcaatgc caggtcggag
     2221 acccgtccgg tcttcaccct gccgctcgcg agcaatccgc tgctcgcagc cggcgtcgcg
     2281 accgcgttgg cactgcacgt ctcggcgatg taccttccac cactgcagcg actgctggac
     2341 gtggcgccgc tcacgatcga cgaatggatc gagatgccgc tcgtcgcgct cacgctgctc
     2401 gtggtgatgg agatccacaa gcgtggttgg gccgtgcgcg cgcgagcggg tgcgacgaac
     2461 tgaccagccg ggccgcgcgg agtcgagcat gcgagggtcg acatgagccc gatacagccc
     2521 ggaatccttg cgccgatccc gagggtcgcg cgccacctga gcttcacgct ggcgccggac
     2581 gccgggcccg ggccggcgct ggagcggctg gcgcaggccg tcgatcctgc ggccacggtc
     2641 gtggggctcg ggcgatcgat ctcactggcg ctcggccgcc atgtcccggg cctgcgcgat
     2701 tttccgtcgg gtgccggccg cggcttcgag atccccgcga cgcccggcgc gctgtggtgc
     2761 tggctgcgtg gcgaagaccg gggcgaactc gtgcaccggt cccgcgccct ccaggcgctg
     2821 ctcgcgccgg cattcgcgct cgattcggtc gtcgacgcgt tcatgcacga agacggccgt
     2881 gacctgaccg gttacgagga cggcacggaa aacccgaccg gcgaacgcgc ggtggcaacc
     2941 gcatcgctgc aaggtgccgc cgctggcctc gacggatcga gttacgcggc ggtccagcag
     3001 tgggtgcacg acttcgccgc gttcgaggcg ctcggtcccg acgagcagga cctgagcgtc
     3061 ggccgtcgtc gcgccgacaa cgaggagatc gacgaggcgc cgccgtccgc gcacgtcaag
     3121 cgcacggcgc aggagagttt ctccccggag gctttcgtgc tgcgacggtc catgccctgg
     3181 gccgagggcg cgaaggccgg gctggtcttc ctcgcgttcg ggcgctcgtt cgacgcattc
     3241 gaggcgcagc tctcgcgcat gaccggcctc gacgacggaa tcacggacgc gctgttccgc
     3301 tttacgcggc ccgtcacggg cgcttactac tggtgtccac cggtatccgg cgggcgactc
     3361 gacctgcggg cgctcgaact gcgctgagtc ggtcgccgcc ggccaccacc cgtggcgctg
     3421 gtcagccgaa gatgcccttg agcaccggcg ccatcctgcg agtgccgccg atcgacaccg
     3481 ccgacgccag tccgcccatc atggcgcctg tcacgccgca cgacatgacg tcctgtccgg
     3541 tgagccacaa gccggggatt ctcgtgcgcg gcctgagcca cgactgccgc atgcgctcgg
     3601 gatcgtggtc gagcccgtag agttcgccgc gcgcatagcc gcagaaccac tgcatggaca
     3661 gcggcgtcga gatctcccag tagtcgatgc gatcgcgcag tgccgggagc ttcgcgtaca
     3721 ggtgttcgag caggcgctcc ccgaaggcct gtttcagggc ctcgtagtcg tcgccgcgct
     3781 tgccccacgt cgtgccggcc catggctcga aaatagagta gggcgccggt gccacgatct
     3841 cgatcgtcga gcggcccgga tggcgacgct cgaaatccgg gtccttcgcc gagggaaagg
     3901 agatgtacac ggccggaaac gggccgtgcg ggtcggcggc gaagcggtcg tacgcggcgt
     3961 cgtagtcgtt gtcgtggtag atccagtagt tggtcttcgg cagcccgagg tcctcggccc
     4021 tgcccttgag cccgatgtag acgcagagat ggccgatgct cggcttcacg cgtgcgagga
     4081 ggcggtcgta cccgacacgc cggcgcgtct cctccgggac gaggtggccg aaggtgttga
     4141 gcgctccagc gtcggagatg acgcagtcgc acgcgatctc actgccgtcc tccatgcgca
     4201 cgccgcgtac tgcgtgcctg cgagccggct ccagcagcac ttcctgcacg cgcgcgtacg
     4261 tgaacacttc cccgcccgcc gcacggatgc gcggcaggat cgtgtccgcg atgcgccacg
     4321 cgccaccgac cggatagaag ccgccgtgca ggtagtgctt tgccaccaat gcctggatga
     4381 cccacgcgga gcgcttcggt ggcaggccga ggtcgcccca ctgcccggtc aggacggcga
     4441 tgagttcggc gtcccgcgtg agttccgaca gcacctccca ggtcgtgcgg ccgaagctgc
     4501 gcggcaatcg gctgcgcagg aacggcccgg cgattgcggc cgcccacggc ggcagcgtcc
     4561 ggtccagcga gaacgtacgc atgccacgcg cggtctcgcc gagcagttcg aggtaccgat
     4621 cgatcgcggc tgcctcccgc gggaagtaac cgacgaggtt gtcgcggtac gcgtcgcggc
     4681 ctgccaccgc atcgtacgtg cgctcgccga tgaagaaccg gtcatagtgc gagtccatcg
     4741 gcgcccagtg caggttgcct tgcgtcagga aatccatcat ccggcgcgac gtcgtcctcg
     4801 cgcccatgtc gccgatgtag tggacgccga catcccactc gtatcccgcg cggtcgtaac
     4861 tgtgcgtggc gccgccggcc gtgtagtgct gctccagcac cgccacgcgc cagccgattt
     4921 ccgacagcag cgcggcggtg gtcagtccgc cgatgcccga cccgatcacg agcgcgtcgt
     4981 accgtccctc gacccgattc gggcgatagc ggcggccgag ccgcagcgtg ctcggcgtca
     5041 ggttgcgcct gggagtctgc atcaccgcat tatggtgcat gccgcgccgc atggtgcatg
     5101 ctgcaccgcc gcgcgcaatc agcggtggcc accctgcgta cggcggcggc ggcgcgtcat
     5161 gtcgggccgc gtgcacgagc ggcgcttgag cggtgcgtca cgaattcgtg cgcccggagt
     5221 gcgcaggagt tgcggtggcg cggcgcggag ttaacgtgca cacgacggag gtgtgcgatg
     5281 actcgcgtcg cgatatgcag gttgcttgcc gggctctatg cctgcgcggc ggcctcgttg
     5341 gcggtggcgg gcgacgacgg ccccgactgg ttccggcgct ccgcgcagcc gctgacgaac
     5401 ctggacatgg agtcggccga cgagttcctc gtcgtgctgc agcgggaccg cgtggacggc
     5461 cccgacctgc tgacgctccg ctacacgctc gggcaggttg gcccgttgtc cacctacgcg
     5521 ggcgccggta tcaaccggac ctcatatttt gcctggtcgg tcaaccccga cgacggcctc
     5581 ctggaccgca gccgtcatcg cgccatcggc gccgcggccg agctgggcgc ggcctggcgc
     5641 gtgagcaaca cgctcgcgct tgccgccgac ctgcactgga tcgaccttgc cagcgacgcg
     5701 gacatgctcc gtgtcggcag tacgatggtc gccgccgatg ccgtggcggt cggcgtctcc
     5761 ctgggctggc gcttccgctg aacgacggac tatcgcgccc agcgcgtgat gcgcaggcgg
     5821 ccgccgtcga gcgtgccggg cgcggtgctc gatacgcgta ccgtgccggt cccgcgccgc
     5881 aggtcgaagt cgccgagctt gatccagcgt gcgccccaga cccgctgatc gagttcgagg
     5941 cgaagtccct cgtccgcgtc gtccacctcg tagagcgcgg cgggcgtgtg ccgcggatcc
     6001 gccgaatacc acaccgctat ctcgtacctg ccgtcggccg gcacgtccag gttcgcggtg
     6061 aacgcaccgt ccggcaatcg cgcgggctcc aggtagcgcg gcgccggcag accctcggcg
     6121 atgccgagtc gtgcgaggac gcgttccgtg aggctgccca ggcgcacgac gtcccacggc
     6181 tgcggttcca gcgggttcca gccgttcgac agtgcagcga ccacgatccg gcggccgttc
     6241 ggcaactccg cgtacatgac gtcctcgagc gtgtcgaagg ctgctccgac cttgttctcc
     6301 tgcagtgatc ccggtggcag gccgccgccg aggctggcct ggtcggaaaa cgtcggtcgc
     6361 ctcagcagcc cccgcatgta tgccgtcgcc tgctgctcga tcgctccggt tgcgaccgcc
     6421 agcatcaggt tcgcggcgcc gttcgaagac atcatgtttc gtcccatctc cttccacgca
     6481 agctcctcga ggccggacgg ctcgctcccg gagttcgtcg gataggtctt ggtgaagaac
     6541 cgctggttcc cgagcaggcc ggcccgatcg agcacccgct ccgtgtagcg ccgccgctcg
     6601 atgaatgcgc cgaggtcggc gccgtcgggt ggcccgttcg gcgctccggt gaccgtgtcc
     6661 acgacccagc cggtggcgac gttgctcgag cgcaggatca tcgggctcac ccactcgtcg
     6721 agacagtcgg ggcgcttgcc ctgcgccgcg caccagtgga ccgcgcccac caggaacggc
     6781 agcttgacgc agctcgccgg ataggcgagc tcgtcgccac cgacactgcc tcgcagccag
     6841 ccgtggtcct tgtcctccac gagcacggtc acctgcaggc ggtcgaagtc ctgcgtcgcg
     6901 aggaactccg agcgtgccgt ctccacggcc tgctgaacgg ccgcgtcgtc cacgatcgtc
     6961 ggaatcgtca ccggcgcggc ccggaccgcc gtcgcgcccg caagcaacgc gagcgcgacg
     7021 aattcgatgg ccagcgcggt cggaacacgc agccgtcgct gcatgacggt gcccgtgagc
     7081 cagaggacgg cgagcacgcc cgcggaccac gcggcgacct gcgcgaaggg gagcgcggac
     7141 gagcgccaga gcagcgttgt tgcggcgagc aggacggcga cgaagtgcag cgcgacgtaa
     7201 caccgtaccg cggacgtcat cggcgggtcg tagcggcgca gcttgtcgag cgcgaacggc
     7261 ggcttgtccc aggccggacc agtctccgtt gcaggatgcc agcccggcgg cttgatccac
     7321 acgagcagcc ggtcgcgcca gcgctgcgca ctccacgact tccgcgccag gtccacgtac
     7381 acctcgaggt tcgcccacag cgggtcccac gaatcgagcg acgcccgcgt gccgtacacg
     7441 cacggttccg tctcttcgac gaatgagccg aacatccggt cccacgccat gaagatgccg
     7501 ccgtagttgc ggtcgatgta gcggtcgttc accgcgtggt gcacgcgatg gttcgacggc
     7561 gatgcgaacc agcggtcgaa ccagccgagc ttgccgacct gctcggtatg gatccagtac
     7621 tggtagagca ggtcgatgat cgcggccacg acgaagacct gcggcgggaa acccgcgatc
     7681 gccatgggaa ggtagaagat ccagccgagg agtgcgcccg agctcgtctg tcgcagcgcc
     7741 gtcgtgaggt tgtactcctg gctctggtgg tgcacgacgt gcgacgccca gaagaccgcg
     7801 ctgacgtgtc ccagccggtg gttccagtag taacagaagt catagacgac gagcccggcg
     7861 acccagaccc accactgctc tgcgggcagg cgccagacgg cgaacgactc gtaggtccac
     7921 acgtagatcc cgaccgcggc ggccttcgtg aacaggccca cgacctgact catgatgccg
     7981 aggctcaggc tgttgaccgc gtcgttcagc cggtagttgt gccgtccccg cgcccgaccc
     8041 acgaggtact cgatggcgat gagcgcgaag aacacggggg tcgcgagcgc gatcgtcttg
     8101 cccatgccgt gctcctcagg gctcgtcgtg ccagccgcgc gatcgctcga ccgcgcgcga
     8161 ccactgtcgc attctcgcag ctgccgactc acgtgacatg gctggttcga agcgccggtc
     8221 ggcccgccag ttcgccgcga cctcgtcggt cgactgccag aagccgaccg aaagcccggc
     8281 gagcgatgcg gcgccaagcg cggtcgtctc ggtgacgcgc gggcgaacga ccggtacccc
     8341 gagcaggtcc gcctggaact gcatcagcag gtcgtttgcg gtggcgccgc cgtccacccg
     8401 cagttccgtg agcggctggc gggcgtcctt ctgcatcgcc gccaatacct cggcactctg
     8461 gaacgcgatc gcctcgaggg cggcacgtgc aaggtgcgca cgcgtcgttc cgcgcgacag
     8521 gccgaccacg aggccgcggg cgtacgcgtc ccagtgcggg ctgccgaggc cggtgaatgc
     8581 gggaacgaga tacacgccgc cggtgtccgg caccgatgcg gccagtgcct cgacgtcggc
     8641 ggagcgctcg atcagcccga gcccgtcgcg cagccactgc acgacggcgc cgccgacgaa
     8701 gaccgaccct tcgagcgcgt agtgccgctc gccggtgtcc cacgcgatcg tggtcagcag
     8761 ccggttcgcc gaatgcagcg gctcgagccc ggtgttcatg agcatgaagc agccggtgcc
     8821 gtaggtgttc ttggccatgc ccggcgcgaa gcagccctgt ccgaacagcg ccgcctgctg
     8881 gtcgccggcg atgccgccga gcggcacttc ctcgccaccg atgactgcga ccgcgtcgcc
     8941 gtccggcaat ctcgacggca cgacgcgcgg caggacccgg cgcggtatgc ggagcagctc
     9001 gagcgcgtag tcgtcccacg ttcccgtcgc gagatcgagc agcagggtgc ggctcgcgtt
     9061 cgacgcatcg gtcacgtgca cggcgccttg cgacagcttc cacgcgagcc agctgtcgac
     9121 ggtgccgaag gcgagttcgc cggcagtcgc gcgcttgcgc agtgcgggat cgctgtcgag
     9181 cagccacttg agcttggtac cggagaaata agggtcgagc agcagcccgg tgcgtcgcgc
     9241 gatctccggc tcgtgcccgt cggatttcag ccttgcgcac agctcggcgg tgcgccggtc
     9301 ctgccagacg atggccggcg cgaccgggcg ccctgacctg cggtcccaca gcaccgtcgt
     9361 ctcgcgctgg ttggtgatgc ccacggcagc gagatccgac ggtgtggcgc gcgccctggc
     9421 cagcacctct gcaatggtcg ccgcctgcgt cgcccagatc tcctcggcgt cgtgctcgac
     9481 ccagccggag cgcggaaagt gctgggtgaa ttcgcgctgc gccgtcgcga cgacctggcc
     9541 gtcgtggtcg aacaggatgg atcgggagct ggtcgtgccc tggtcgaggg cgagcacgaa
     9601 tcggcgcatg cggcggacct ctgttgccga agcgtactac atcgcggtcg cggcggactc
     9661 agcccgcggc atagtccgcc ggccgcgcca ccgggttccg cacgagccac tcctcgaggc
     9721 gttcggcggc gtcggcgggc aggtgcaggg cgagcttgct gcggcgatag aggatgtcct
     9781 ccgccgtgag cgcccactcc tcgcgtcgca ggtagtcgat ttcacgcgcg tgcaggcccg
     9841 gcagcacttc ctcgccgagc gcggagatcc cacgtgcgtc gcccagcatg cgacgcacgc
     9901 gtgtgccgta cgcggccgcc atgcgatcga gcaggccatc gggcagccat gggtactcgg
     9961 cgcgcacgct gcgacgaaac gcggcgtagt ccgcacgcgc gatgtcgccg cctggcagcg
    10021 gcgcccccgc cgtccagtgc cgcctcgacc agcccatgcg tgacgcgagc ccgtcgagca
    10081 cgtcttccgc gagcttgcga taggtcgtga gcttgccgcc gtagatcgac agcagcggcg
    10141 ggccctgctg gtccacgtcg agcacgtagt cgcgcgtgac gctggtggcg acgtccgacg
    10201 gatcggccag cagcggcctg agccccgcga acgtccactt cacgtcggcg tggcccacca
    10261 tgcggcggaa ataccggttc gacatggata gaagataggt gacttcggct ggttcgatcg
    10321 acggcgtcgc aaggtcgccc gtgtagtccc gctcggtcgt gccgacgagc gtgcagtccc
    10381 cctcgtaggg gatcgcgaac acgatgcgcc ggtccggccc ctggaagatg taggcgtggt
    10441 cgtgctcgaa caggcgcggc acgacgatgt ggctgccctt gacgaggcgc ggatggcgtc
    10501 gcgcgggcac gggtgtcgcc tcgtcgagga atcggtccac ccacgggccg gtggcattga
    10561 cgaccgcggc ggccgagatg cgcgactcgg cgccatcggc gtgcgccagg gtcgcggtcc
    10621 agcggtcccc ggagcgctcg agccgcacgc atcgcgtgcg cgacaggacc gtcgcgccac
    10681 gctcgcgtgc gtcgagggcg gcgagtacga cgagccgggc gtcgtccacc gcggcgtccg
    10741 agtacacgaa gccctgggtg tagcgcgggt ccagcgccgc gcccgcggga tgccggcgca
    10801 ggtccactgc tctcgacgcg ggcagcaggc gccggcgcgc caggtgatcg tagagataca
    10861 gccccgcgcg gatcatccac gccgggcgca ggtgctcgtc gtgcggcagc acgaagcgca
    10921 tcggccgcac gaggtgcggc gccgagcgca gcacgatctc gcgctcgcgc agcgacttgc
    10981 gcaccagccg gaaatcatag aactcgagat acctgaggcc gccgtggatc agcttggtgc
    11041 tggcggacga cgtgtgcgcc ccgaggtcgt gctgctcgca gagcagcgtg ccgaggccgc
    11101 ggcccgccgc gtccctcgcc accccgacac cgttgatgcc gccaccgacg accaggagat
    11161 cgtactgtcg ggctcctccg ctcatcgtgc gccgtcctca gccgtagatc atcgcggacg
    11221 agcgctggtc cagccaggcc tcctcgtcgg cgaccgccag cgcatcgagg tcggccgagg
    11281 ccgcggcggg gtcgtccacc cagcggcgca gcaggtcgct gccgttgatg aggtcgatgg
    11341 cgagacggtc atgctcgtac tcgtagggga agtggcgcca cagctcgaat tccgggtgca
    11401 gccgccgcag cgccttgaac gcgagcgcct gcagccgcca cggccggaac gactcgtgcc
    11461 cgtacgagcc gtcctcgacg tggtaatgca ggccggcgca cagttgcccg gcgtgcttgt
    11521 gaaacgtcgg ctcgaaccag caggctcgga gccggcagcc cgcgagccat gcgggcgcga
    11581 ggcggcgcat ttcggccacg atcgcgggga tgtcgagccc cggcgcgccg aacagctcca
    11641 gcggacgggt cgtcccgcgt ccctcggaca acgtcgtgcc ctccagcatc accgtgccgg
    11701 gatagcagcg cgccatccag ggattcggcg cattcgggct cgggttcacc cacacacgct
    11761 cgccgagcgg ccatccgtag cccggaccgc gcgacgggtc ccagccctgc atggcgatca
    11821 cgcggtaatc gacgtcgagc cgcagccgtc gcacgaacca gtgccccatc tcgccgagcg
    11881 tgaggccgtg gcgcatcggc atttcgccgg cgccgacgaa actctcccat ccctgcctga
    11941 gcgtcagtcc ttccactggc cggccgatcg gattcggccg gtcgagcacc cacacggact
    12001 tgcggtgctg cgcggcggcc tcgagcacgt agcgcagcgt cgtgacgaag gtgtagatgc
    12061 ggcagccgag atcctgaagg tccacgagca gcacgtcgaa cgtgtcgagc atcgccggcg
    12121 tcggtcgccg gacctcgccg tagaggctga acacggggat gccgtgccgt gcgtcgcgga
    12181 agtccggcga ttcgaccatg ttgtcctgct tgtcgccgcg cagcccatgc tgcgggccga
    12241 aggcggcagc gaggcggatg tcacccagcg ccgcgatcgc atccaatgag tgcgtgaggt
    12301 ccgccgtgac cgacgccgga tgggccagca gcgcgaccct gcgaccggcg agaggccggc
    12361 gctggtccgg ttctgcgagc agccggtcga taccgaacct caccacgagg tggcccgctc
    12421 gatctccagc acgaagccgg caggactgtg gaaatcgggc ttctccgcgc gatgcgccag
    12481 cgcccagtac gacaggcgcc cgtcccggtc ctccaccacc gccgcgaggc tcacgagcag
    12541 catgccgtca cccggcagcg cgagcagagc ctcgagcggg atgatggcct cgagtgcgat
    12601 gcggtcctcc gccacgctca cggaaaccgt cggtggcgtg ctgtactgca gcggcaccat
    12661 gccggcgcga taggcggaga aaccgtacgc ggcccactcg gtggagggcg agaagttgaa
    12721 ttcgcagtac gcgcgcgagc cctggccggc gatgaacgct tcgaagcagg tgtgttgcca
    12781 caacccgtcc gcctgtcgcg gcgcggcgtg tggcggcagc gtgatggcgg ccacgtcgcc
    12841 gtcgaggaag tacctgagcc tcaggtcgcc gtcgccggtc gattcgagtt cgacgtcgat
    12901 ccgttgcacg gccggcgccg gcgtcgaggg atgacgcgtc agggcaacgc agcgtggggc
    12961 gaggccgggc atccgaccat cataccgggc gccgatccgg ctgtagccta gagggccctg
    13021 agcacggtgg ggtccggtcg ttccagccag tggaggaacg cgtcggccag tctctggcac
    13081 tcgcggatcg cgcggtccca gtcgcggatg cggccggcgt ggtcctggcc gtagcggtaa
    13141 aaatccttcc ggtccggcaa cttaccgttg ggcagcgagg ccaggaacga gcgtgacggc
    13201 gcgaccagca gcatgtcgtc gagccacgga tgaccgcggg acttgcgccg ccacggcagg
    13261 tgcttgtcga gccagccggg cgtcacatag tcgttgaagt gcgggtacag gacgatgcgt
    13321 cggcgcgggc cgctccgtgt cagccgctcg tacggcagct gcaggtgata gtcgacgagc
    13381 gcgccgtccc agtagttgcc cgggggcgcg ccggcgatcc cgcggaccgg ggcgcagacg
    13441 atgggtatcg tgccggacgc caccagcgcg tcctcggtgt tggccgggtc cagcgcgacg
    13501 acccgcgatc cgaactcgtc gaagggagaa gctaggaagg tccttccagt tgaaaacacc
    13561 acgcgctcga ggtgggccgc gaggctgcgc cgcgagatcg cgttggcgag gccggcccgc
    13621 ccgaacgcga gagtggattc cgttccctcg agcgcgccgc gtgcacgcga agtcacgatc
    13681 gccagggtca cgccgggccg gagccgcagt ggcgcatcgc cgagcacggc gcgcgccagg
    13741 cgccggcacg cgcgcgcgac gtcgtccggt gtcggcttgc ggtcgtagtt ctggtcgtgg
    13801 acgtagctgt accgcagccg ctcgagcgcg gcgaccgggt ccggttgtgc aagcgccgcc
    13861 atgcgccatg cgccgaccga cgccccgacg agctcgatcg gcgcggtgtc cttgagccac
    13921 tcgcgataca acaactggtc gagcggaatc agcgcgagcc ctttcgggcc gccggccgcg
    13981 gccgggatgc acgcgacgtc cgccgggacg acgccattgg cgcggacgtg ctccgcggcg
    14041 ccgggtccgg cgagcagttc gattacggct tcgcgtcgat tcactcggct cacagcaggt
    14101 cgcgcaggcg gtaccacagc ataccgagga ccagcgccgg agtgcgcagt gcgcgaccgc
    14161 ccgggaacac gcggtgcgga acgtggccga acaggtcgaa tcgctcggcc tgacccgcga
    14221 ccgcatccgc ggcaagccgc cccgccatgc cggccattgc gatgccgtgg cccgaaaatc
    14281 cctgcaggta gagcacgttc ggcgcgatgc gcccgaagtc gggcacgcga ttcatcgtga
    14341 tgtccacgta gccgccccag acgtagtcga gccgtgcgtc ggcgagttgc gggaacacgc
    14401 gcagcatccg cgcccgcgtg gcatggccgg tattgcgcgc gtcgatcccg gaataactga
    14461 cccgcccgcc gaaaagcagg cggtggtcgg ccgagcaccg gaaatagtcg agcacgaaat
    14521 tcacgtcggc caccgcgacg ttctcgcgca ccagttcgcg gatgcgcgcc tcgccgagcg
    14581 gctgtgtcga gaccacgtag gtgccgaccg gcatcacgcg ccagtcggcc acggtgcgca
    14641 cgccgtcgag gtaaccgccg cgcgcgaaga tcacgtgatc ggcgtcgacg tggcctgccg
    14701 acgtcgacag gcgcacggtg ccgccgtgcc ggaccgacct cactcgactc gcttcgaaga
    14761 gtcgcgcgcc ggcggtctcg gcggcgcgtg ccaggcccag ggtgtagttg agcggatgca
    14821 ggtggccgct gccggggtcg tacaggcccg cgcagtaccg ccgcgtcgcg agcagcgcct
    14881 cgacgtccgg gcgatcgagc atgcgagtgc cggcgtagcc gtagcgctcg ccgagttcgg
    14941 cgtgcaacgc ctcgagttcc cgccgctggc gctccttggt cgcgacgtgc aggtgccccc
    15001 agtgcaggtc gcagtcgatc cggtgctcct ccacccggtg acgcagcagc gccagcgcct
    15061 cgacggacac gtcccacatc agtcgcgccg cctcgggacc cacctggtcg acgaccacgt
    15121 cctggctcgc gccgaacccg aagatggcct gcccgccgct ccgacccgaa gcgccccagc
    15181 ctacgcgccg ggcttcgagc agcaccaccc ggtagccacg cgaggcgagg tccagcgcgg
    15241 ccgagcaacc tgcgatgccg cctcccacga cgcacacgtc cgcccgcacc tggccggcca
    15301 gtgcgggtcg tggcggggcg gggtgtgccg tggcggcata ataggaagcg acgtgctgca
    15361 tggggcccga ctgtagcgtc gtccggcccg cgcgggtaat tgtgattggc acggatcgcg
    15421 gcaagcggca agcttggcag catgcaggac ttcgatctcc atgcgccctg gccgtcccag
    15481 gccgacgccg tgcccgtgtc caagcgcggc cgggacctga tccgcgaccc gctgctcaac
    15541 aagggcacgg cattccgcgc cgacgagcgt gagcggttcc ggctgcacgg attgctgcca
    15601 tcacggcaga tctcgatgga cctgcaggtg cagcgcaacg tcgcctcgat cgatcgcgcg
    15661 gcgactcccc tcgaccagta cctcaggttg tcggccctgc aggatcgcaa cgagcacctc
    15721 taccaccgcg tgctcgcgga gaacctcgac cggttcatgc cgatcgtcta cacgccgacg
    15781 gtggccgagg cgacgcgcaa cttcagccag gtgttccgcc gcgcgcgggg tgtgtggctg
    15841 acaccggatt tccgcggccg gatggcgcgt gtgctgtcgg acgcggcggg cgacgaaccg
    15901 gtgcggctga tcgtcgcgac cgacaacgag tccatcctcg gcatcggcga ccagggcgcc
    15961 ggcggcatgg cgatctgc
//
LOCUS       DAOMFD010000031        15664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_805_length_15664_cov_30.156448, whole genome shotgun sequence.
ACCESSION   DAOMFD010000031 DAOMFD010000000
VERSION     DAOMFD010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15664
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_805_length_15664_cov_30.156448"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..778)
                     /locus_tag="PL152_03320"
     CDS             complement(<1..778)
                     /locus_tag="PL152_03320"
                     /inference="COORDINATES: protein motif:HMM:NF013707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HQR48337.1"
                     /translation="MAQPAKAPDRVNDFVIKLANVNGTGSGSANTLLMKSIFRMGVPV
                     MGKNYFPSNIQGLPTWYEIRVTKDGYVARSGRVDLMVAMNAETYHKDVREVSPGGFLI
                     YDSTWPRPTVLTREDITVLGVPLARLCNENFVGVRNRILMKNVMYLGVLAALLEIDVE
                     VIRQLLTESYAKKQALVDANMKAVQLGYDWAKQNLPCPLPVRVERMDKTKGHIMIDGN
                     TAAGLGAVYAGATVGPWYPITPSTSLMDAFKSFCAKLRVDP"
     gene            complement(745..2556)
                     /locus_tag="PL152_03325"
     CDS             complement(745..2556)
                     /locus_tag="PL152_03325"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR48338.1"
                     /translation="MQPTDTSNPDYFHRVVDCQWACPAHTDVPEYIRLIALGRFSDAY
                     MVNRESNVFPGILGRVCDRPCEPACRRGRVEEKPVAICRLKRVAADHKDDIVERLPVV
                     PRQGNGKRVACIGAGPASLTVANDLLPLGYEVVILEQWDKPGGLMRTNIPAFRLPEAV
                     LAEEIGYITQMGAEIRYNSRVDSLKKLLATGGFDAVFVGSGAPKGKELKLAGRTEGDA
                     NIHIGIDWLESVAFKHVEKIGERVLIIGVGNTAMDCCRSSLRLGAQDVKVMARKPRQF
                     FKASEWELEDAEAEAVEIVVNHAPKSFVVENGRLAGMLFDRMEYDFDARGRIVGERVV
                     GEAFFPADDVILAIGQENAFPWIEDDLGIQFDKWHVPVVDKVTFQSTRPDVYFGGDAA
                     FGPKNIIWAVEHGHQAAISIHNFCAGQPVTNRLPPTMNLSSRKMGLHEWSYKNDYNPV
                     ERRLVPHVSLKERFKKISIEVELGFDPEQTAAEVQRCLNCDVQTVFEAKLCIECDACI
                     DICPTDCLTITRDGEEQELQTRLKAPRRNPDEPLYVSEPLKQTGRVMVKDEDLCVHCG
                     LCAERCPTAAWDMQKSKINLPHANDHTWPSPQKRQTA"
     gene            2849..3289
                     /locus_tag="PL152_03330"
     CDS             2849..3289
                     /locus_tag="PL152_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48339.1"
                     /translation="MKGAAWMGLLAAWPLACAAGESRTTFLVTAQVTPRVSLETLSEP
                     AQFAVSEADAARGYVDVTATYQVRNNDPAGYVLRLEPRVGYASGVVVTGIAAPVTLFD
                     ETVEVIAPAALSTRMLTLTFRVALPLAAGAGDYPLPLRISAATL"
     gene            complement(3290..3835)
                     /locus_tag="PL152_03335"
     CDS             complement(3290..3835)
                     /locus_tag="PL152_03335"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HQR48340.1"
                     /translation="MPINDFLAARRRGRRALAVAWTALIAVLACLAIFIPLRAHAAAD
                     CSVTVTGVAFGTYDPTISAADDSTGTVNVTCTYTGGGATGIAYSVRFSRGSSNSYSQR
                     QMRAGTLSLNYNLYRDAARSVVLGDGTGGTSIFSGSFTVGPGVGNRTRTGTHTVYGRV
                     PASQPVDPGSYSDPIVVTLTF"
     gene            complement(3835..6132)
                     /locus_tag="PL152_03340"
     CDS             complement(3835..6132)
                     /locus_tag="PL152_03340"
                     /inference="COORDINATES: protein motif:HMM:NF012786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015473 - fimbrial usher porin activity
                     [Evidence IEA];
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HQR48341.1"
                     /translation="MRLRLAVAVALALAGSAAHAAPAPEAYGESIIEITLNGERMPEP
                     LVVRRGLDGTLLVRAEDLKSLRLKPPAHGALTVNGERYYRIDSEIGATVSFDDTTQSV
                     DVNVPAQAFVPSTSVLASADTPRAAQAQSGGFVNYDVSAERVDGHASGGGYLEAGVFG
                     SQGVLTASAVARTGEDLPGAARLDTTWTRDFPDRMTTLRVGDAVSSSGSWGRAARFAG
                     VQYGTNFSTQPTFVTTPLLSATGDAVLPSTVEVFVNGNPVASQDVQPGPFQIEGVPAV
                     NGAGQMQVVVTDALGRQQVIAQPYYAGNALLRAGLSEYSVEAGAIRQDYAVKSNDYGD
                     VVGAATYRHGINDRLTAEVHAEGQSDGTAAGGAGAALQVGNLGIVSGTAAVGGSGDGL
                     GWLTGLGFEHSGPVLSLYARMLYASESFSQLGSNTSGTRPRSRTFGGVGLNLGGYGSL
                     QLAYGRQENWNGPGAQTLGLGYSLTLGALGSLNLFASHYDSADSGTDFLLTWTMPFGE
                     RGSASATLQQNSGGGGSQEGFSAVASVQQNLPVGSGSGYVATLSTNDDYHLGYAYQGH
                     AGLVSADYARANGQDGTRIGAVGGVAISGLGVMPSQRLDQSFAMVKVADYAGMQVYLD
                     NQPVGRTDAKGRVLLDNLLPYQTNQVSIDPRELPMDATISSPTMTVTPAYRSGVSVAF
                     PVSRADSMTMRLLQADGQPVPAGATVMLEGKPFPVGLDGLVYVSGIATNAHAAVTWRD
                     GKCAADFVRPSNGGPVPDLGDVPCR"
     gene            complement(6137..6877)
                     /locus_tag="PL152_03345"
     CDS             complement(6137..6877)
                     /locus_tag="PL152_03345"
                     /inference="COORDINATES: protein motif:HMM:NF012565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HQR48342.1"
                     /translation="MLRYAAIISVLASAAAFGLPPASAGSFSISPLRAELSQQATTAA
                     ITVRNMDAAPVIVQASTFAWSQPDGEDHLEATKDLLVSPAVFTIPPNGSQLVRVALRR
                     DADPRRELAYRLTLQEVPPQAAPDFTGLTVALKLSLPVFVAANSPSPAQLDWSAARGA
                     DGAVVLGARNDGETHAKILNVTLTPLSGDAATLQQSVAAYVLPGVSRSWTIRNENDKN
                     GSTAAADRYRLKANTERGEVDAELTVGR"
     gene            complement(6887..7393)
                     /locus_tag="PL152_03350"
     CDS             complement(6887..7393)
                     /locus_tag="PL152_03350"
                     /inference="COORDINATES: protein motif:HMM:NF017079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spore coat U domain-containing protein"
                     /protein_id="HQR48343.1"
                     /translation="MRRHSIAPVAAAVLLALAGGAQAATKTTTFNVSANVASNCLIST
                     TNLGFGAYTGVAQLTGTSTVGVRCTNGTGYTVKLSAGNGGSFAPRLMSDGASHSLQYN
                     LYTTAANLVVWGDGTGSTGVQGATGTGLSNAGNVDYTVYGTLPDNAVNQTAPVGSYSD
                     VITVSVDY"
     gene            complement(7705..10683)
                     /locus_tag="PL152_03355"
     CDS             complement(7705..10683)
                     /locus_tag="PL152_03355"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQR48344.1"
                     /translation="MDQRAPLPLHRLHDEEIERLLASGERRAELVALFGPQGYRELSA
                     LARKASATRLRAGRVVYVLPGLMGSRIGTRGRLLDDVLWLDPIEIAAGHLTRLALPRG
                     ARLTALGAMLLNALKLKLSLRIAGFDSRLHAYDWRRSVAVLATELEERIAADGAEDAM
                     LVGHSMGGLVARAALGANTGRIARVVQLGAPNAGSFAPVLALRAVYTTVRKLAALDLR
                     HDAEELARLVFRTLPALHELLPDPRLAGGADLFDLGNWPRDALRPDPQLAAAAEAARK
                     RWPGADARCLHIVGVRQETVVGAELRKGSFRFRVDTDGDGTVPRVLAEIPGAAAWFVS
                     EKHGGLPNNGRVIAAVVDLLRTGRTDRLPSATRRVKARRPRIIHEATLRRVAPHKVHW
                     QDLSADARRRLLEPVVSPEFHGAVSKRSLRAPTATRGDAIVAPSKRVLEVRLANGSIV
                     DANARALVLGVFRNVDPSGAAAAVDARLDGTVREFTLRRMISGRLGETFVLPCARSPL
                     LAEYVLFAGLGDFSGFSVEEHAFAAENAVRTLVRARTPDFATVLFGTGSGVPVATALE
                     RQLSGIFAALRAADGDRIIRRITICEVDPRRYVSLRKALPRAVARAAGDDFSVVTDET
                     AFAPSPGLPTRDARAQAAADPVYLLASLRESGRGAYECSTSLLTAGAKAAVLSGTMHV
                     GAASLRATLARAGPDHLTNEDMAGYGEALASRLLADTVRDGLASMSRRPLVIVHDREA
                     SRVPWETLCVDGVHPALEGGLSRRYQSDDLTVARWREDSPQEGRPRVLMVVNPTLDLP
                     GAAREGAALRDELLRGGVQADCLEGADASLARVLAETGSGRYDALHFAGHGFFAADSP
                     GRSGLVCADGEILRGVDLARLAGLPALVFFNACEAARVRKGLPDWRRRLADASRAHGV
                     AEAVLDGGVANFLGTHWPVGDDAALVFAGCLYERLLDGASLGDAVLESRRRVKAAGSI
                     DWADYVHYGNPAFRLGTATD"
     gene            complement(10706..11167)
                     /locus_tag="PL152_03360"
     CDS             complement(10706..11167)
                     /locus_tag="PL152_03360"
                     /inference="COORDINATES: protein motif:HMM:NF016221.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HQR48345.1"
                     /translation="MIDRTPGRLAALLLATLPVLAWAAGPTISDAWARATPPGLDVGA
                     AYLTITGGAAADRLVGVSTPVAAMAHLHTMDDAGGMATMRAVDGIDVPPGKRVVLAPG
                     GLHVMLMGLTKALVAGQSFPLTLEFAKSGRQTVSVMIRPAGDAGPAAPVHH"
     gene            complement(11164..12066)
                     /locus_tag="PL152_03365"
     CDS             complement(11164..12066)
                     /locus_tag="PL152_03365"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQR48346.1"
                     /translation="MNLRDFRYLVALADERHFGRAAQRCHVSQPTLSAQLRKLEEYLG
                     VPLVERQPRRVTLTEAGTRVVERARRLLLEADAIVELTRTDRDPLAGALKLALIPTVG
                     PYLLPHVARRLRRDLPRLKLMLYEYQTAPLLEKLRSGELDLGILALPVPLDGLEAQPL
                     YEEPFTVAVPAGHPLAARDRVKLDDLKGETLLLLEDGHCLRDQALEVCSRVKVHEDQD
                     YRATSLETLRQMVAAGHGVTLMPQLATESQAGPARGLRFKSFVKPAPARTIGAVWRKS
                     TTRAPAIEAVADSIRAVMKEESSR"
     gene            12116..12550
                     /locus_tag="PL152_03370"
     CDS             12116..12550
                     /locus_tag="PL152_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HQR48347.1"
                     /translation="MTPRRQRLSAGVVVVRPSAEGWRFLLLRAFNHWDFPKGMVEDGE
                     EPLAAAVREVREETCIADLEFAWGEGSTSTGPYSRGKVARYYIASTQTAEVDLAVNPE
                     IGRPEHTEYRWVSFGEAGRLVSPRLKPVLQWVAQVLNLPAGE"
     gene            12604..13134
                     /locus_tag="PL152_03375"
     CDS             12604..13134
                     /locus_tag="PL152_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48348.1"
                     /translation="MIHRLLIAMLLLVASGASMAACVERDNPPSAAALEKAAVLDVMD
                     SYLAAVSSGNLAAMKQLQTPEGMTYRAKSSADGSVEVVPHPNSWWVDPARKEGHALRE
                     RYWAPTVLVRGSIAVVWAPYEFWIDGKTSHCGIDSFDFVKVGGRWLVANAMWTVEPDS
                     CPDLRPRSPTELRPAD"
     gene            13197..14390
                     /locus_tag="PL152_03380"
     CDS             13197..14390
                     /locus_tag="PL152_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VCBS repeat-containing protein"
                     /protein_id="HQR48349.1"
                     /translation="MRTRTGAAMLCSLGLTACGGGGGGSGGGGDLYPLWVETDVAVAD
                     LDGDGRPDILTLAQYASSDKAREGRLIVHLQRTPGVFTTVQTAIVGVYPWRMAVADID
                     GDGALDVAVSDVDGDRGAWLLVQDRANRGQFLPAREIAAGFNGYDIAVADLNGDTLAD
                     IAVTSSAARLAILYQDPLQPAAFQPAASITVQGTATTAVTTGDLDGDGRADLAMNVIQ
                     SASGGAVNSVTGISLQQGDGSMGPVTTLAPQQGINVTGMTIADYDGDGFTDVFAYFTP
                     SSTSYRSKMMVLLQGPVPGAFAAPADTSMGDLKGEMDAVFADLDGDQRPDAAVVGFYP
                     SGSPTEVKANLHRFTQSGAGAFALVSTTPLSIDASCVTAGDLDGDGLNELVVLADKDR
                     FQVLD"
     gene            complement(14404..14844)
                     /locus_tag="PL152_03385"
     CDS             complement(14404..14844)
                     /locus_tag="PL152_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48350.1"
                     /translation="MEAVHPSNPPPGEHPEHPAIVAFVRTTLGCQCPDDVFRAVAVER
                     VGARNGGIAHVRLTVGDRLLIYVFEPGQAGAAAEVVESLARTGIAERNARGFNRFRLV
                     IATPNPRGFPGDAPATFAAVAGPDARAHLHFVATSGLPPALRSP"
     gene            complement(14844..>15664)
                     /locus_tag="PL152_03390"
     CDS             complement(14844..>15664)
                     /locus_tag="PL152_03390"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HQR48351.1"
                     /translation="REVVLLNKLRITREIIEATPALRLIAVTATGTNNIDLVAAAERG
                     IAVCNLRDYCTASVVQHVFGTLLMLTQRLREYDALVRSGAWQRGDQFCLLDFPIRELT
                     GRTMGIVGFGVLGRGVAYAAAAFGMNVVVANRPGGERQAGRVDLEDLLPRADVLSLHC
                     PITPATQGMIGARELARMKSDAVLINTARGALVDPVALAEALRAGRLGGAAIDVLEQE
                     PPVNGNPLLAGDIPNLIVTPHVAWAAREARQRCLDELAANVEDFRRGGHRGRVA"
BASE COUNT         2333 a         5299 c         5592 g         2440 t
ORIGIN      
        1 ccgggtcgac gcgcagcttc gcgcagaacg acttgaacgc gtccatcagc gacgtggacg
       61 gcgtgatcgg gtaccagggg ccgaccgtgg cgcctgcata caccgccccg aggccggccg
      121 cggtgttgcc gtcgatcatg atgtggccct tggtcttgtc catgcgctcc acccgcaccg
      181 gcagcgggca gggcaggttc tgcttcgccc agtcgtagcc gagctgcacc gccttcatgt
      241 tggcgtccac cagcgcctgc ttcttcgcgt agctctcggt gagcagctgg cggatcacct
      301 cgacgtcgat ctcgagcagc gccgcgagca cgccgaggta catgacgttc ttcatcagga
      361 tgcggttgcg cacgccgacg aagttctcgt tgcagaggcg cgccagcggc acgccgagca
      421 cggtgatgtc ctcgcgcgtg agcaccgtcg gccgcggcca ggtcgagtcg tagatcagga
      481 agccgccggg ggatacctcg cggacgtcct tgtggtaggt ctccgcgttc atggccacca
      541 tgagatccac ccggcccgag cgcgccacgt agccatcctt cgtcacccgg atctcgtacc
      601 acgtgggcag gccctggatg ttggacggga agtagttctt ccccatcacg ggcacgccca
      661 tgcggaagat ggacttcatc agcagcgtgt tggcactgcc ggagccggtg ccgttcacgt
      721 tggcgagctt gatgacgaag tcgtttacgc ggtctggcgc ttttgcgggc tgggccatgt
      781 gtggtcgttg gcgtggggca ggttgatctt cgacttctgc atgtcccagg ctgccgtcgg
      841 gcagcgctcg gcgcacagtc cgcagtgcac gcagaggtcc tcgtccttga ccatgacccg
      901 gccggtctgc ttgagcggct cggacacgta cagcggctcg tcgggattgc ggcgcggagc
      961 tttcaggcga gtctgcagct cctgctcctc gccgtcgcgg gtgatggtca ggcagtcggt
     1021 cgggcagatg tcgatgcagg cgtcacactc gatgcagagc ttcgcctcga acaccgtctg
     1081 cacgtcgcag ttcaggcagc gctgcacctc ggccgccgtc tgctcgggat cgaagccgag
     1141 ctcgacctcg atcgagatct tcttgaagcg ctccttcagg ctcacgtgcg gcacgagccg
     1201 gcgctcgacc gggttgtagt cgttcttgta cgaccactcg tgcaggccca tcttgcgcga
     1261 ggacaggttc atcgttggcg gcagtcggtt cgtcaccggc tggccagcgc agaagttgtg
     1321 gatcgagatc gcggcctggt ggccgtgctc gaccgcccag atgatgttct tcgggccgaa
     1381 ggccgcgtcg ccgccgaagt agacgtcggg gcgggtggac tggaaggtca ccttgtccac
     1441 caccggcacg tgccacttgt cgaactggat cccgaggtcg tcctcgatcc aggggaacgc
     1501 gttctcctgc ccgatcgcga ggatcacgtc gtcggccggg aagaaggctt cgccgacgac
     1561 acgctcgccg acgatgcggc cgcgcgcgtc gaaatcgtat tccatcctgt cgaacagcat
     1621 gccggcaagc cgcccgttct cgacgacgaa gctcttcggc gcgtggttca ccacgatctc
     1681 gacggcctcg gcctcggcat cctcgagttc ccactcggag gccttgaaga actggcgcgg
     1741 cttgcgcgcc atcaccttca cgtcctgcgc gccgaggcgc agcgaggagc ggcagcaatc
     1801 catcgccgtg ttgccgacgc cgatgatcag cacgcgttcg ccgatcttct cgacgtgctt
     1861 gaaggccacc gactcgagcc agtcgatgcc gatgtggatg ttcgcgtcgc cctccgtgcg
     1921 cccggcgagc ttgagctcct tgcccttcgg cgcgccggag cccacgaaca ccgcgtcgaa
     1981 tccgcccgtg gccagcagct tcttcaggct gtccacgcgc gagttgtagc ggatctccgc
     2041 gcccatctgc gtgatgtagc cgatctcctc ggcgagcacg gcctccggca ggcggaacgc
     2101 cgggatgttg gtgcgcatca ggccgcccgg cttgtcccac tgctcgagga tgaccacctc
     2161 gtagccgagc ggcaggaggt cgttggccac cgtgagcgag gcgggacccg caccgatgca
     2221 cgcgacgcgc ttgccgttgc cctgcctcgg gaccaccggc agccgctcca cgatgtcgtc
     2281 cttgtggtcc gcggccacgc gcttgaggcg gcagatcgcg acgggtttct cctcgacgcg
     2341 gccgcgccgg caggcaggct cgcacggccg gtcgcagacc cggccgagga tgcccgggaa
     2401 cacgttcgat tcccggttca ccatgtaggc gtccgagaag cggccgaggg cgatcaggcg
     2461 gatgtactcg gggacgtcgg tgtgcgccgg gcaggcccac tggcagtcca cgacccggtg
     2521 gaaatagtcc gggttcgacg tgtcggtcgg ttgcacgagc ggtggcgccc aaaagtcagg
     2581 gagaggtgcg gcagcttagc ggttttgagg gggggtgcaa cccgccgccg gcggccattg
     2641 ttgcgacgca gcggccggtc gccgggccgg gtgtcccgac ctgttgcccg gcgaggggtg
     2701 gcaattcggt taaatcttgc gccgtcgtcg agccgatggg gttaccttgt gacgcaattc
     2761 aggaaatcgg gtccgcaagc cggcggtgat cggcagcgga acgggacggg taaggtcgcg
     2821 ccggtttcgg ccggcggagg gggcggggat gaagggcgca gcctggatgg gtctgcttgc
     2881 ggcctggccg ctcgcgtgcg cggccggcga gtcgcggacg acattcctgg taacggcgca
     2941 ggtgacgccg cgcgtgagcc tcgagacgct gagcgaacca gcacaattcg ccgtcagcga
     3001 agcggatgcc gcgcgcggtt acgtggacgt cacggcgacc taccaggtgc ggaacaacga
     3061 tcccgcgggc tacgtactgc gcctcgaacc gcgcgtcggg tacgcatccg gggtcgtcgt
     3121 aactggcatt gccgccccgg tcacgctgtt cgacgagacc gtggaggtga tcgcacctgc
     3181 agcactctcg acccggatgc tgacgctgac ctttcgtgtg gcgctgcccc tggctgccgg
     3241 cgcaggggac tacccgctgc cgctgcgcat ttccgcagcg acgctctagc tagaacgtca
     3301 gcgtgacgac gatcggatcg gagtagctgc cgggatccac cggctgcgac gcggggacgc
     3361 gcccgtacac cgtgtgcgtc ccggtgcgcg tgcgattgcc cacgccgggg cccacggtga
     3421 aggagccgct gaagatcgac gttccaccgg tgccgtcgcc gagcacgacc gagcgcgccg
     3481 cgtccctgta gaggttgtag ttcagcgaca gcgttccggc gcgcatctgc cgctgggagt
     3541 agctgttgct gctgcctctg gaaaaccgga ccgagtaggc gatgcccgtc gcgccgccgc
     3601 cggtgtaggt gcaggtcacg ttgacggtgc cggtggaatc gtccgcagcc gagatcgtcg
     3661 ggtcgtaggt gccgaaggcg actccggtca ccgtgacgct gcagtccgcg gcggcgtggg
     3721 cacgcaaggg aatgaagatc gcaaggcatg caaggactgc gatcagcgcc gtccacgcga
     3781 cggcgagtgc ccgccgcccg cgtcgccggg ccgcgagaaa gtcgttgatg ggcatcaacg
     3841 gcacggcacg tctccgaggt ccggcacggg accgccgttc gacggtcgca cgaagtccgc
     3901 agcgcacttg ccgtctctcc aggtcacggc tgcgtgcgcg ttcgtcgcga tgccgctcac
     3961 gtacacgagt ccgtcgaggc ccacggggaa tggcttgccc tcgagcatca cggtcgcacc
     4021 ggccgggacc ggctggccat cggcctgcag gagccgcatc gtcatcgagt cggcgcggct
     4081 caccgggaac gcgaccgaca cgccgctgcg gtaggccggc gtcaccgtca tggtcggcga
     4141 ggaaatcgtc gcgtccatcg gcagctcgcg cgggtcgatg ctcacctggt tcgtctggta
     4201 cggcaacagg ttgtcgagca acacgcggcc cttggcgtcg gtccgaccca ccggctggtt
     4261 gtcgaggtac acctgcattc ccgcgtaatc cgccaccttg accatggcga aactctggtc
     4321 gaggcgctgc gagggcatca cacccaggcc cgagatcgcc acgccgccga ccgcgccgat
     4381 ccgcgtcccg tcctggccgt tggcgcgcgc gtagtcggcg ctgacgaggc ccgcgtggcc
     4441 ttgatacgcg tagccgaggt ggtagtcgtc gttggtggag agcgtggcga cgtaaccgga
     4501 gccggacccg accggcagat tctgctgcac ggacgcaacg gccgagaagc cttcctgcga
     4561 gccgccaccg ccggagttct gctgcagcgt ggcgctggcc gagccgcgct cgccgaacgg
     4621 catggtccag gtgagcagga agtccgtgcc ggagtcggcg gagtcgtaat ggcttgcgaa
     4681 gagattcagc gagcccaagg cgccaagcgt cagcgaatag ccgaggccga gcgtctgcgc
     4741 gccggggccg ttccagttct cctggcgacc gtaggcgagc tgcaggctgc cgtagccccc
     4801 gaggttgagt ccgactccgc cgaatgttcg gctgcgcggg cgcgtcccgg aggtattgct
     4861 gccgagctgc gagaatgact ccgacgcata gagcatgcgc gcgtagaggc tcaggacggg
     4921 gccgctgtgc tcgaagccga ggccggtgag ccagccgagc ccgtcaccgc tgccacccac
     4981 ggccgccgtc ccgctcacga tcccgaggtt cccgacctgc agtgcagcac cggcgccacc
     5041 ggccgccgtg ccgtcggatt gaccttctgc gtgcacctcg gcggtgaggc gatcgttgat
     5101 gccgtggcgg tacgtcgcgg cgccgaccac gtcgccgtag tcgttgctct tcacggcgta
     5161 atcctggcgg atcgcgcctg cctcgaccga gtactcggag agccccgcgc gcagcaacgc
     5221 gttgcctgcg taatagggtt gcgcgatgac ctgctggcgg ccgagtgcgt ccgtgacgac
     5281 gacctgcatc tggcccgcgc cattcacggc cggcacgccc tcgatctgga acgggccggg
     5341 ctgcacgtcc tggctcgcga cggggttgcc gttcacgaag acctcgaccg tggagggcag
     5401 caccgcgtcc ccggtggcgg acagcagcgg ggtcgtgacg aacgtcggct gcgtgctgaa
     5461 attcgtgccg tactgcacgc cggcgaagcg tgccgcacgg ccccagctgc cgctcgagga
     5521 aacggcatcg ccgacgcgca gcgtcgtcat gcggtccgga aagtcgcgcg tccacgtcgt
     5581 gtcgaggcgc gccgcgcccg gcaggtcctc gccggtgcgc gcgacggcgg atgcggtgag
     5641 gacgccctgc gagccgaaga cgccggcctc caggtagccg ccgccgctcg catggccgtc
     5701 gacgcgctcg gccgagacgt cgtagttcac gaatccgccc gactgcgcct gcgcggcacg
     5761 cggcgtgtcg gcggaagcga gcacgctcgt cgaaggcacg aatgcctgcg ccgggacatt
     5821 cacgtccacg ctctgcgtcg tgtcgtcgaa gctgacggtg gcgccgatct ccgagtcgat
     5881 gcggtagtag cgctcgccgt tgaccgtcag cgcaccgtgg gcgggcggct tgaggcgcag
     5941 tgacttgagg tcctcggcgc gcaccagcag cgtgccgtcc aggccgcggc gcacgacgag
     6001 cggctcgggc atgcgctcgc cgttcagcgt gatctcgatg atcgattcgc cataggcctc
     6061 cggtgcgggg gcggcgtgcg cggcgctgcc cgcgagggcg agcgcgacgg cgaccgcgag
     6121 ccggaggcgc atggggctac cgtccgacgg tcagctccgc gtcgacctcg ccccgctcgg
     6181 tgttggcctt caacctgtag cggtccgcgg cggcggtgct gccgttcttg tcgttttcgt
     6241 tcctgatcgt ccacgaccgg gacacgcccg gcaggacgta ggccgccacg gactgctgca
     6301 gcgtcgctgc atcgcccgag agcggcgtga gtgtgacgtt caatatcttc gcgtgcgttt
     6361 cgccgtcgtt cctcgcgccg agcacgaccg ctccgtcggc gccgcgggcg gccgaccagt
     6421 cgagctgcgc aggcgagggc gagttcgcgg cgacgaacac cggcaggctc agcttgagcg
     6481 ccacggtcag gccggtgaaa tcgggggccg cctggggcgg aacttcctgc agcgtgagcc
     6541 ggtaagcgag ctcgcgccgt ggatcggcgt cccgcctgag cgccacgcgc acgagctggg
     6601 acccattcgg cggaatcgtg aacacggccg gcgacaccag caggtccttc gtggcctcga
     6661 ggtgatcctc accgtccggc tgcgaccacg cgaaggtcga ggcctgcacg atcaccggtg
     6721 cggcgtccat gttgcgcacc gtgatcgccg ccgtcgtcgc ctgctgcgag agctcggccc
     6781 ggagcggcga gatggagaat gatcctgccg acgccggcgg cagcccgaag gctgccgccg
     6841 acgcgaggac gctgatgatt gcagcgtaac ggagcatcgc ggacggtcag tagtcgaccg
     6901 aaaccgtgat gacgtcggag tacgatccga ccggcgccgt ctggttcacc gcattgtccg
     6961 gcagcgtgcc gtacacggtg taatcgacgt tgccggcgtt cgacagcccc gtgcccgtgg
     7021 ctccctggac gcccgtgctg cccgtgccgt cgccccacac gaccaggttg gcagcggtcg
     7081 tgtacaggtt gtactgcagc gagtgggatg cgccgtccga catcaggcgc ggggcaaagc
     7141 tgccgccgtt accagcgctc agcttcaccg tatagcccgt gccgttcgtg cagcgcacgc
     7201 cgacggtgga ggtgcccgta agctgtgcga cgcccgtgta cgcgccgaag ccgaggttgg
     7261 tggtcgaaat gaggcagttc gatgccacgt tcgcgctcac gttgaaggtg gtcgtcttgg
     7321 tcgcggcctg cgcaccgccg gcaagcgcca gcagtactgc ggctgcgacc ggtgcgatcg
     7381 aatgtctacg catgttgcat cctctgtgtg atggttgggt cttctgcgcc ccttcctcct
     7441 cgctgccggc gtgccggtgg acgatgacga gtccgggact caacgattcc ttcgcgagac
     7501 gcagggtttc ttcaggccct tcggcgcact ccggcttcgc ttgccggaat tgttttttgt
     7561 gcccgcgcag gcatgcgtga cggggctcgc ggttccttat gtcaaccgtc gtaaccgggg
     7621 gcgcaagccc ggcgaagtcg gaagcgcgtc acattttccg ggccatggcg ccgcccggcg
     7681 aggccggccg attggcgggc ccggtcagtc ggtggcggtc cccaggcgga atgcgggatt
     7741 gccgtagtgg acgtaatcgg cccagtcgat ggagccagcc gccttcaccc tgcgtctgga
     7801 ttccagaacg gcatctccca gtgacgcacc gtcgagcaaa cgttcataga ggcagcccgc
     7861 gaagacgagc gccgcgtcat cgccgacggg ccagtgagtg ccgaggaaat tcgctacccc
     7921 gccgtcgagg acggcctctg cgacaccgtg cgcgcgactc gcgtccgcca gtcggcggcg
     7981 ccaatcgggc aaccccttgc gcacgcgtgc cgcttcgcac gcgttgaaga acacgagtgc
     8041 cggcaggccc gcaagccgcg caagatcgac gccgcgcagg atctcgccgt ctgcgcacac
     8101 gaggccgctg cgcccggggc tgtccgctgc gaagaaacca tgtcctgcga aatgcagcgc
     8161 gtcgtaccgg ccggagcccg tctccgccag cacgcgggcg agcgacgcgt ccgcaccttc
     8221 caggcagtcc gcctggactc cgccgcgcag caactcgtca cgcagcgccg cgccctcgcg
     8281 tgcggcgccg ggcaggtcga gcgtcgggtt cacgaccatg agcacgcgcg ggcggccttc
     8341 ctgcggggaa tcctcgcgcc agcgggcgac ggtcaggtcg tcgctctggt accggcggct
     8401 gagtccgccc tcgagcgccg gatgcacgcc gtcgacacac agcgtctccc acgggacgcg
     8461 cgacgcttca cggtcgtgca cgatcacgag cggccgacgg gacatcgacg cgagcccgtc
     8521 gcgcaccgtg tcggcgagca gccggctcgc gagcgcctcg ccgtagccag ccatgtcctc
     8581 gttggtcagg tggtccggcc cagcacgcgc cagcgtcgcg cgcagcgacg ctgcgccgac
     8641 gtgcatcgtg ccgctcagca ctgcggcctt ggcgccggcc gtcagcaacg acgtcgagca
     8701 ctcgtacgct ccgcggccac tctcgcgcag ggatgccagc aggtacaccg ggtcggcggc
     8761 agcctgtgcc ctggcgtcac gcgtcggcag cccgggcgac ggcgcgaacg cggtctcgtc
     8821 cgtgacgacc gaaaagtcgt caccggccgc tcgcgcgacg gctcgcggca gcgccttgcg
     8881 cagcgacaca tatcgtcgcg ggtcgacctc gcagatcgtg atgcgtcgta tgatccggtc
     8941 gccgtccgcg gcgcgcaggg cggcgaagat ccccgagagc tggcgctcga gtgccgtcgc
     9001 gaccgggacg cccgatcccg tgccgaacag caccgtcgcg aaatccggcg tccgcgcgcg
     9061 gaccagggta cgcacggcat tctcggccgc gaatgcgtgc tcctcgacgc tgaagccgct
     9121 gaaatcgccg aggccggcga acagcacgta ctcggcgagc agcgggctgc gcgcgcaggg
     9181 gaggacgaag gtctcgccga ggcgtcccga gatcatgcga cgcagggtga actcgcggac
     9241 agtgccatcg aggcgcgcgt ccaccgcggc tgccgcaccg gatgggtcga cgttgcggaa
     9301 cacgccgagc accagcgcgc gggcattggc gtccacgatg ctgccgttcg cgagacgcac
     9361 ctcgaggacg cgcttcgagg gtgcgacgat cgcgtcgcca cgggtggctg tcggcgcgcg
     9421 gagcgaccgc ttcgacaccg cgccgtggaa ctccggggat acgacgggtt cgagcagccg
     9481 gcgccgggcg tccgcggaca ggtcctgcca gtggaccttg tgcggcgcga cccgccgcag
     9541 ggtcgcttcg tggatgatcc gcggccgcct cgccttcacg cggcgagtcg cggacggcag
     9601 gcgatcggtg cggccggtgc gcagcaggtc caccaccgcg gcgatcacgc gcccgttgtt
     9661 gggcaggccg ccgtgcttct ccgacacgaa ccacgcggcc gcacctggaa tctcggccag
     9721 cacacgcggc accgtgccgt cgccgtcggt atcgacgcgg aagcggaacg aacccttgcg
     9781 cagttcggcc ccgacgacgg tctcctgccg cacgccgacg atgtgcaggc acctcgcgtc
     9841 cgcgccgggc cagcgcttgc gcgcggcctc ggcagcagcg gcgagttgcg ggtccgggcg
     9901 cagcgcgtcg cgcggccagt tccccaggtc gaacaggtct gcgccgccgg ccagtcgggg
     9961 gtcgggcagc aattcgtgca gcgccggcag cgtgcggaag acgagtctcg cgagttcctc
    10021 ggcatcgtgc cggagatcga gcgcggcgag cttgcgcacc gtggtgtaga ccgcgcgcag
    10081 cgcgagcacc ggcgcgaacg aacccgcatt tggcgcgccg agctgcacca cgcgtgcgat
    10141 gcggccggta ttcgcgccga gcgccgcccg tgccacgagg ccgcccatgc tgtggcccac
    10201 gagcatggcg tcctcggcgc cgtcggccgc gatgcgctct tcgagttcgg tcgcgagaac
    10261 cgcgacgctg cgtcgccagt cgtaggcatg caagcgcgaa tcgaacccgg cgatgcgcag
    10321 cgacagcttg agcttgagcg cgttgagcag catggcgccg agcgccgtga ggcgtgcgcc
    10381 acgcggcagg gcgagccggg tcaggtggcc cgcggcgatt tcgattgggt cgagccagag
    10441 gacgtcgtcg agcaggcggc cacgcgtgcc gatgcgcgag cccatgaggc cgggcagcac
    10501 gtacacgacg cggcccgctc gcagccgcgt cgcgctggcc ttccgggcga gcgcggagag
    10561 ctcgcgatag ccctgcggcc cgaacagcgc gacgagctcg gcgcggcgct cgccgctggc
    10621 gagcaggcgc tcgatctcct cgtcgtgcag ccgatgcagg ggcagtggcg cgcgctggtc
    10681 catggcccgt gcgccagccg ggtcgtcagt gatgcacggg ggccgcgggc ccggcgtccc
    10741 cggcggggcg aatcatgacg gacaccgtct gcctgccgga tttcgcgaac tcgagcgtca
    10801 gcgggaatga ttgccccgcg acgagcgcct tcgtgagtcc catgagcatg acgtgcaggc
    10861 cgccgggggc aagcaccacg cgcttgccag gcgggacgtc gatgccgtcc accgctcgca
    10921 tcgtcgccat gccgcctgcg tcgtccatcg tgtgcaggtg agccatcgcc gccaccggcg
    10981 tgctcacacc gacgagccgg tccgcggcgg cccctcccgt gatcgtgagg taggcggctc
    11041 cgacgtcgag gccgggtggc gtggcgcggg cccacgcgtc ggaaatcgtc ggccctgccg
    11101 cccaggcaag cacgggcagc gtggcgagca acagggcggc gaggcgtccg ggtgtgcggt
    11161 cgatcatcga ctcgactcct ctttcatcac ggcgcgtatc gagtcggcga ccgcctcgat
    11221 agcgggcgcg cgcgtcgtgg acttgcgcca cacggccccg atcgtacgcg ccggcgcagg
    11281 cttgacgaag gacttgaatc gcagtccgcg cgcaggcccg gcctgagatt cggtcgcgag
    11341 ctgcggcatg agggtgacgc cgtgacccgc ggcgaccatc tgccgcaggg tctcgaggct
    11401 ggtcgcccgg taatcctggt cttcgtgcac cttgacgcga ctgcagactt cgagcgcctg
    11461 gtcgcgcagg cagtgcccgt cctcgagcag cagcagcgtc tcgcccttga ggtcgtcgag
    11521 cttcactcga tcgcgagcgg cgagcgggtg ccccgccggc accgcgaccg tgaacggctc
    11581 ctcgtacagg ggctgtgcct cgaggccgtc gagcggcacg gggagcgcga ggatcccgag
    11641 gtcgagctcg ccggagcgca gcttctcgag cagcggtgcg gtctggtact cgtacagcat
    11701 gagcttcagg cgcggcaggt cgcggcgcag gcgccgcgcg acgtgcggca gcaggtacgg
    11761 cccgaccgtg gggatgagcg cgagcttgag cgcgccggcc agcgggtcgc gatcggtgcg
    11821 cgtgagttcg acgatcgcgt cggcctcgag cagcaggcgc cgtgcccgct cgaccacacg
    11881 cgtgccggct tcggtgagcg tcacccgtcg cggctggcgt tcgacgagcg gcacgccgag
    11941 gtactcctcg agcttgcgca actgcgccga cagcgtcggc tggctcacgt gacagcgctg
    12001 cgcagcgcgc ccgaagtgcc gttcgtcggc gagcgccacg aggtagcgga agtcgcggag
    12061 attcatggcg tgatcatagt ccgggaccgc gatcggtttc ctctatactt tgcaggtgac
    12121 tccgcgacgc cagagactgt ctgccggtgt cgtcgtcgtg cggccgtcgg ccgagggatg
    12181 gcgattcctg ctgctgcgcg cattcaatca ctgggacttt cccaagggca tggtcgagga
    12241 cggcgaggag ccccttgcag cggccgtgcg cgaggtgcgc gaggaaacct gcatcgcgga
    12301 cctggaattc gcgtggggcg aggggtccac ctccacgggt ccgtacagcc gcggcaaggt
    12361 cgcgcgctat tacatcgcga gcacgcagac cgccgaggtc gatctcgcgg tgaacccgga
    12421 gatcgggcgg ccggagcaca ccgagtaccg ctgggtgagc ttcggcgagg cgggccgact
    12481 ggtctcgccg cgcctcaagc cggtgctgca gtgggtggcc caggttctca acctgccggc
    12541 cggcgagtga aagggccggc cggtagcgga ctcgtgtccg gcggtacgac ggagagacgg
    12601 tcgatgatcc acagactgct gattgccatg ctgctgctcg tagcgtccgg cgcgtcgatg
    12661 gcggcgtgcg tggaacgcga caatccgccg agcgcggctg cactcgagaa ggcggcggtc
    12721 ctcgacgtga tggacagtta ccttgccgcg gtctcgtcgg gcaacctcgc ggcgatgaag
    12781 cagttgcaga caccggaggg catgacctat cgggcgaagt cgtccgccga cggcagcgtg
    12841 gaggtcgtgc cgcatccgaa ttcctggtgg gtggatccgg cccgcaagga gggtcacgct
    12901 ctgcgcgaaa ggtactgggc gccgaccgtg ctcgtacggg gcagcatcgc cgtggtctgg
    12961 gcgccctacg agttctggat cgacggcaag accagtcact gcggcatcga ttcgttcgat
    13021 ttcgtgaagg tcggcggccg ctggctggtg gcgaacgcga tgtggacggt cgagccggac
    13081 tcgtgcccgg accttcggcc caggagcccg acggagttgc ggccggcgga ctagcggcag
    13141 agatcctcgc gtccaatacc gatgcgcgcg gcgaacccgc agcgccacaa tcagccatgc
    13201 gcacgcgtac cggcgccgcg atgctctgtt ccctcggact gaccgcatgc ggtggtggtg
    13261 gtggcggctc gggcgggggc ggcgacctgt atccgctatg ggtcgagacc gacgttgccg
    13321 tggccgacct cgacggggac ggacgtcccg acatcctgac actcgcccag tacgcgagca
    13381 gcgacaaggc acgggaagga cggctgatcg tgcacctgca gcgcacgcct ggcgtgttca
    13441 cgaccgtcca gactgcgatc gtcggcgtgt atccctggcg catggccgtg gcagacatcg
    13501 atggtgacgg cgcgctcgac gtggcggtgt ccgacgtgga cggagacagg ggggcctggc
    13561 tcctggtgca ggaccgggcc aatcgcgggc agttcctgcc cgcgcgcgag attgccgccg
    13621 gattcaacgg ctacgacatc gcggtggcgg acctgaacgg cgacacgctg gcggacatcg
    13681 ccgttacgtc cagtgccgca cggctggcga tcctgtatca ggacccgctg cagccagcag
    13741 cgttccagcc tgctgcgtca atcaccgttc agggcaccgc gacgaccgcc gtcaccacgg
    13801 gcgacctgga cggcgacggc cgcgcggatc ttgcaatgaa cgtcatccag tccgcgagcg
    13861 gcggcgcggt gaacagcgtc accgggatca gcctgcagca gggtgacggc agcatgggtc
    13921 ccgtgacgac gctcgcgccg cagcaaggta tcaacgtcac cgggatgacg atcgccgact
    13981 acgacggcga cgggttcacg gatgtgttcg cgtacttcac gccgtcgtcg acgtcgtacc
    14041 ggtcgaagat gatggtcctg ctgcaggggc cggtaccggg agctttcgcg gcgccggcgg
    14101 acacctcgat gggtgacctg aaaggcgaga tggacgccgt gttcgccgac ctcgacggcg
    14161 accagcggcc cgatgccgcc gtggtcggtt tctatccgtc gggctccccg acggaggtga
    14221 aggcgaacct gcatcgcttc acgcagtcgg gcgccggcgc ttttgcgctg gtctcgacga
    14281 ccccgctgtc gattgatgca agctgcgtca ccgcaggcga cctcgatggc gacggcctca
    14341 acgaactcgt cgtgctcgcg gacaaggacc gtttccaggt gctggactga ggcccctgcg
    14401 ccgtcagggg ctgcgcagcg cgggaggcag gccgctggtg gcgacgaagt gcaggtgggc
    14461 tcgcgcgtcc gggccggcga cggctgcgaa ggtggcggga gcgtcgccgg ggaagcctcg
    14521 cgggttcggc gtggcgatca ccagtcggaa gcgattgaac ccgcgcgcat tacgctcggc
    14581 gatgccggtc cgcgcgagac tctcgacgac ctctgctgcg gcacccgcct gcccaggctc
    14641 gaacacgtag atcagaagcc ggtcaccgac cgtgaggcgg acgtgggcga tgccgccgtt
    14701 ccgggcgccg acgcgttcga cggccaccgc cctgaacacg tcgtccgggc actggcagcc
    14761 cagcgtcgtg cgcacgaatg ccacgatcgc cgggtgctcc gggtgctcgc cggggggtgg
    14821 attcgagggg tgcacggctt ccatcaggcc acgcgtcccc tgtgaccgcc ccgccggaag
    14881 tcctccacgt tggcggcgag ctcgtcgagg cagcgctggc gcgcctcgcg cgcggcccag
    14941 gcgacatgcg gcgtgacgat aagattcggg atgtcgcccg cgagcagcgg attgccgttc
    15001 accggtggct cctgctcgag tacgtcgatg gccgctccac cgagccggcc ggcgcgaagc
    15061 gcctcggcca gcgcgaccgg atccacgagc gcgccccgcg cggtgttgat cagcaccgcg
    15121 tccgacttca tgcgggcgag ttcgcgggcg ccaatcatgc cttgggtcgc cggcgtgatc
    15181 gggcaatgga ggctcaggac gtcggcacgg ggtagcaggt cctcgaggtc gacgcgtccc
    15241 gcctgtcgct caccgccggg acgattcgcg acgacgacgt tcatcccgaa cgccgccgcc
    15301 gcgtatgcga cgccgcggcc cagtacgccg aagccgacga tacccatcgt gcgcccggtg
    15361 agttcgcgaa tgggaaagtc gagcaggcag aactggtcgc cacgctgcca cgcaccggat
    15421 cgcacgaggg cgtcgtattc gcgcagccgt tgcgtgagca tgagcagcgt gccgaacacg
    15481 tgctgcacga ccgaggccgt gcagtaatcg cgcaggttgc acaccgcgat gccgcgctcg
    15541 gccgcggcaa ccaggtcgat gttgttcgtg cccgttgcgg tgaccgcgat cagcctcaag
    15601 gccggcgtcg cctcgatgat ctcgcgcgtg atccgcagct tgttgagcag cacgacctcg
    15661 cggt
//
LOCUS       DAOMFD010000032        15489 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_823_length_15489_cov_30.928081, whole genome shotgun sequence.
ACCESSION   DAOMFD010000032 DAOMFD010000000
VERSION     DAOMFD010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15489)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15489)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15489
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_823_length_15489_cov_30.928081"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="PL152_03395"
     CDS             complement(<1..377)
                     /locus_tag="PL152_03395"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HQR48352.1"
                     /translation="MTWLVVGLAAFLFIHSVSIVAPAWRDSMAARLGELRWKRIYSIV
                     SLAAFVALVHGCSLARQEPVVLYQPTAWMRHLAFVVMLPAFPLLLSAYLPGRIKDAVK
                     HPMLAATQVWAVAHLLANGMLADV"
     gene            complement(374..1366)
                     /locus_tag="PL152_03400"
     CDS             complement(374..1366)
                     /locus_tag="PL152_03400"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQR48353.1"
                     /translation="MDQLVAMRVFTRVAERGSFAQASDELDISRAAASAHVAALEKHL
                     GARLLNRTTRRVSLTAEGTDYLRRARRILDEIADAEETLRGTRSKPQGPLRVDVPVSF
                     GRYLLLPALPEFVRRYPAIELDVRLNDRVVDVVADRVDVALRVGGLKQSGLVARRIAQ
                     VNVVTCASPAYLAEHGEPRTPDDLRNHRLLGVTPPGGGSPDWQFPPPYTSKRLKLHFA
                     MQFNAAEAPTIAAASGLGIARTADLIAAEYLARGELRLVLKDFIVPGPPISLVYPSAG
                     HQSAKVRVFSDFAADLMLRWTETVRRWLQGGAGASGEVIPARLDRGPLVAGRGS"
     gene            1476..1601
                     /locus_tag="PL152_03405"
     CDS             1476..1601
                     /locus_tag="PL152_03405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48354.1"
                     /translation="MTNQNNLQNRLLSGAIAALAVSILVVFASLTHTVAALATHV"
     gene            complement(2093..3217)
                     /locus_tag="PL152_03410"
     CDS             complement(2093..3217)
                     /locus_tag="PL152_03410"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQR48355.1"
                     /translation="MKPVRIVLVVAALMVAAYLLFSPVPIQPVAWHAPRSEGFTGPYA
                     RNERLADARLVSVAPEVGPEHIVFGPDGKLYTGVLSGAVLRMNPDGTGIETFVNTGGR
                     PLGMDFDRDGRLVIADAMKGLLVAAADGSVTVLADHVGSDPISYADAVVVADNGRMYF
                     TDATRRFSPVDLGTFDAALLDVMEHSCTGRVLEYQPATQQVRVVIDGLCFPNGVALSG
                     DQAKLFIAETGEYRIWKVDVDADALDAKAAARDPVNAQAMVVLANLPGFPDNLTRGTN
                     GRLWTGLTKPRSETIDRLSDRPWLRAMSLRLPKSLWPVPPAYGHVLAFDEDGRVVADL
                     QDPSGRLPETSGVTEREGDLYVQSLHADALGVLPASSINR"
     gene            3309..4481
                     /locus_tag="PL152_03415"
     CDS             3309..4481
                     /locus_tag="PL152_03415"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48356.1"
                     /translation="MDELRLNPAWYRPEHLDFRETVRRFVAREVLPHVSDWDEAGSFP
                     RELYRRAAEIGLLGLGFPEEYGGISGTDVFHRLVASIELAQCGSGGLVASLMSHTIGS
                     PPVALLGTPEQKARVLPAVLSGGKIAALAITEPSGGSDVAALRTSAVRDGDHYVVNGE
                     KTFITSGMRADYLTVAVRTSPAGSGAGGISLLLVEGDTPGLERTALRKMGWWASDTAH
                     LRFTDCRVPARNLLGAEGAGFRSIMQNFNAERFTLAAGAVGFAWVCYQEALEWARERR
                     TFGQRLADHQVVRHRLVDMLSRIEAARTMLEEAAWRLEHAPAGDPGLVASLCMLKNVA
                     TQAMQYCADAAVQTLGGMGYMRGTHSERIYREVKVNMIGGGTEEIMKELAARQLGL"
     gene            4586..6100
                     /locus_tag="PL152_03420"
     CDS             4586..6100
                     /locus_tag="PL152_03420"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR48357.1"
                     /translation="MKQLSGMDHVFLKLEHGNQFMHVAGLGIYDPSTAPGGKVRFKDI
                     LAFFEARLDSAPVFRRRLVSVPMDLDRPYWIEDDDVDVEFHVRHVALPHPGDWRQLCI
                     QVARIHSRPLDRSKPLWEAYVIEGLDNIPGIPRGSFAFYTKFHHAAIDGEGGTVVLQA
                     IHSFAPDEAVPDDVRRTRVRDREPTQLELYTRAIVNNLQRVPKAASFTLQTARRLVGA
                     GAGYMGQVRAMLQEAGLPSAETVKSRLRRPPVTRFSGKVSPHRVVELVALPMADIKQV
                     RQKIEGATINDVFMTTVGGALNRYLAAKGELPDRTMTAQVPMTLRGENKGGDQGNQVG
                     VTVMPLHTEVADPIERLHAIKQGAAKSKALVAVVGKDLAKNLYDLLPAVASEIFTTRV
                     MLPTMNIVVSNVRGPDVPLYMAGAQMVAFAPVSIAMNGLGLNVTGFSYHGTLWICAVA
                     CRDMMPDPAFFADCLRKSFADLATAASHAGHAAKGEPRTKTGRAPKGARPARKR"
     gene            complement(6110..8299)
                     /locus_tag="PL152_03425"
     CDS             complement(6110..8299)
                     /locus_tag="PL152_03425"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HQR48358.1"
                     /translation="MIPRQLVAWAGLATLAAATFASAATQPTEAQLKLAQPLVAPWTG
                     PYGGVPPFDKVRTDEIGPALEVGMDRNLAEVDLIATNPNPPTFENTIAALERTGRALT
                     RAATIYNVYTSTMNDDAVQAVEREMAPKLAAFQDQITQNEALFKRIEAVYEVREKSGL
                     TAEQQRLTWWYYTNFVRAGAKLDAAAKKRLADINQELAGLYTKFSQNVLADENNGVVL
                     IDKPADLAGLPASIRDAEAEAAASRGKKGQWAVLNTRSSVEPFLTYADNRALREKVWR
                     MFVSRGDQGGATDNNATITQILKLRDERAKLLGYANHAAWRLEDSMAQNPERAMGLMM
                     AVWPAAVARVHEEVADMQAVADKEHAGIKIEPWDYRYYAEKVRKEKYDLDETQITPYL
                     QLENLREGMFYMARELFGFRFTPVDPKKVPVYYPDVRVWEVTDDKGGLVGLWYWDPYA
                     RKGKQSGAWMNAYRNQERFDGEVRTIVSNNSNFVKGKPGEPVLVSWEDATTLFHEFGH
                     ALHGLSSNVSYPTLSGTNVARDYVEFPSQLLEHWLSTPEILNRYALNYQTGKPIPPEL
                     VAKIKKAEKFNQGFKTVEYLSSAIVDMKLHLAASSGPIDPDQFEKATLAEINMPSEIV
                     MRHRTPQFLHIFSGDGYSAGYYSYLWSDTITADAWESFLENGGPWDKAQAAKLKALLS
                     QGNTVEPADAYRQFRGRDPDVGALMRKRGFPEPAKADAAKPATTPSN"
     gene            complement(8341..8736)
                     /locus_tag="PL152_03430"
     CDS             complement(8341..8736)
                     /locus_tag="PL152_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48359.1"
                     /translation="MSAGRVLRWTVGGVVCALAAGCASDDGYGGYGGDASVGVSYGYY
                     SGGYYPYYYPGYPCCWYDDDGRPIEVPPGERPDRPTTLPGKPTQLPAERPSQPAARPA
                     PTPRPSTGMSRPAPRPTPAPRPMGGGRRR"
     gene            complement(8733..9638)
                     /locus_tag="PL152_03435"
     CDS             complement(8733..9638)
                     /locus_tag="PL152_03435"
                     /inference="COORDINATES: protein motif:HMM:NF037170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR48360.1"
                     /translation="MRITPGIGGALVALLGLLAGAPTRAAEPARYWPPITDEPTQRHD
                     PGRFVWVELLTLDVGTAAQFYGKVFGWTFETYGPKDDFKTYTLVLSGGTPIGGMVYVR
                     PRQREKYPSSRWVGFISVGDVAASAALVERGGGKVVVSPRKVGGRGTEALLMDPEGGI
                     FGVITSATGDPADFLSGNDQWLWAELWAADAAKMADFYRGLGPYEVVAGAARGEGDGF
                     QLWSGGYARAGILPRPAQVPTTWLPYVQVKDVGDTVARASAAGGKVVMQPAKMHGTTV
                     AVMLDPTGAPFAVAEWPPVAQEARQ"
     gene            complement(9698..10057)
                     /locus_tag="PL152_03440"
     CDS             complement(9698..10057)
                     /locus_tag="PL152_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48361.1"
                     /translation="MSAPGTSRVLGVALPAVALLLLAAHFQRAGLYPLVAVSIALIGL
                     SLVDDAWARRVLQVALFLGAAEWLRTAWVLAAHRAAMGLPYARMLAILCAVAAITLVA
                     AVVARADSRDGSAAGSN"
     gene            complement(10054..10416)
                     /locus_tag="PL152_03445"
     CDS             complement(10054..10416)
                     /locus_tag="PL152_03445"
                     /inference="COORDINATES: protein motif:HMM:NF020469.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1840 domain-containing protein"
                     /protein_id="HQR48362.1"
                     /translation="MIARSQSRGRRGVIVRFSTRIGQLTMHGDAAVTLLRGMGHTGTV
                     PGAILAADLPDALARLVRMLETSGGSPSPATQSPSGDDEDEREREPVITLQKRAVPLL
                     DMVRTAIARDSDLMWERL"
     gene            10451..11254
                     /locus_tag="PL152_03450"
     CDS             10451..11254
                     /locus_tag="PL152_03450"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR48363.1"
                     /translation="MEFQNLVCERRDGVLAVTLNRPQKLNSLSLGLLKDLKSCAESIQ
                     RDRTVRAVLLTGAGRGFCAGADLTDPDSIPSAGQSMGQLLASRLRDYYHPAALMWSNL
                     PVPLVVALNGVAAGAGASLALFGDITVAAQSATLTFVFAPKLGLVPDMGATYFLARRI
                     GEARAREVALSGRKIGAEEAARIGLIAECVDDARLPTRAEEIAQALASGPREAFVGVR
                     HLMAMAARAGLAEQLEAEAALQARLGDSPDFVEGVTAFREKRAPRFGAG"
     gene            complement(11278..11808)
                     /locus_tag="PL152_03455"
     CDS             complement(11278..11808)
                     /locus_tag="PL152_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48364.1"
                     /translation="MDKPVVNNRDGMALVDPWRDAAPVCRVYSGYAPFRKLLEAYLGG
                     AVKLTDSPERGAREGLLVDCTGTMDSLALATEKFPTHPIIAVLSEADTMVMMEALAAG
                     ADGVIALKDPPETWRECVHVVLGGGRWLRGPGLDVSLEDSFSSYDIATHQRHNGDVTL
                     RTKLFVRNRLADKTGS"
     assembly_gap    11887..11986
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12098..13072
                     /locus_tag="PL152_03460"
     CDS             12098..13072
                     /locus_tag="PL152_03460"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR48365.1"
                     /translation="MTAWGAEGMTSGFLAMRRHALRDVRLVAAALLACAALPVAAATP
                     AATETAPKPRTMGEILDASKPSDWRPLVAENTLYVQLDSGRVVIELAPAFAPLHAANI
                     RKLVRAGYFDGLPIVRSQDNYVVQWGDPDGKRSLGDASGTLPPEFTVAWTPALPFTVL
                     PDVDGYAPQVGFSDGFPAGRDPKTRQAWLAHCYGMVGVGRGNELDSGNGSELYVVIGN
                     APRHLDRNIALVGRVVEGMELLSTLPRGSGPLGFYEKPEQYVPIRSIRLAADVPAAER
                     PNLEVIRTDTATFEALVESRRNRRDDWYKVPAGYVELCNVPIVVRSRP"
     gene            13234..14940
                     /locus_tag="PL152_03465"
     CDS             13234..14940
                     /locus_tag="PL152_03465"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HQR48366.1"
                     /translation="MDRFWLKNYPPGVPADVDVTQYSSLVQLMEEAFAKFGKRTAYAY
                     MDRYFTFDDVDRHSRALAAWLQARGLARGARVAIMMPNVVQYPIAVAAILRAGYVVVN
                     VNPLYTPRELEHQLKDSGAEAIVILENFATTLEQVVAKTSVKHVVVATMGDMLGFPKG
                     LIVNYVVRRVKKMVPAWSLPGHVRFTAALDAGARLKLAPVQATRSDIAFLQYTGGTTG
                     VSKGATLTHGNLVANVLQMEAWVEPALKDTSRGPVPDQLVYICALPLYHVFALTVNCL
                     GGLRLGSMNVLIVNPRDMAGFVKEIAKYKFHVIPGVNTLFNGLAENAEFRKLDFSSLR
                     IANGGGMAVQKAVSDKWLALTGIPIIEGYGLSETSPVATTNPITSREWNGTIGLPVPN
                     TEIAILDDAGQPVPLGQPGEIAIRGPQVMAGYWQRPDETAKVMTPDGFFKSGDIGIMD
                     ERGYTKIVDRKKDMIIVSGFNVYPNEIEAVIATHPKVLECAAIGVADERSGEAVKVFI
                     VRKDPSLTEAEIAEFCREQLTGYKKPKFIEFRTELPKTNVGKILRRALREPVVSEVAR
                     SA"
BASE COUNT         2453 a         5204 c         5307 g         2425 t
ORIGIN      
        1 acgtcggcga gcatgccgtt ggcgagcagg tgagccacgg cccagacctg tgtcgccgcc
       61 agcatgggat gcttcaccgc atccttgatg cgtccgggca ggtaggccga cagcagcagg
      121 ggaaaggcgg gcagcatgac cacgaatgcg aggtggcgca tccacgcggt cggctggtag
      181 agcacgacgg gttcctgccg ggcgaggctg cagccgtgga cgagcgccac gaacgccgcg
      241 agcgagacga tcgagtagat ccgcttccag cggagctcgc cgagccgtgc cgccatcgag
      301 tcgcgccacg cgggtgcgac gatcgacacc gagtggatga agaggaatgc cgcgagaccg
      361 accacgagcc aggtcatgag ccacgtcccg cgacgagtgg accacgatcc agccgagccg
      421 gtatcacctc gcccgacgcg cccgcgccgc cctgcagcca gcggcgcacc gtctcggtcc
      481 agcgcaacat gaggtccgcg gcaaagtcgg agaagacgcg caccttcgcg gactggtgcc
      541 cggccgacgg atacacgagc gagatcggcg ggccgggcac gatgaagtcc ttgagcacga
      601 gcctgagttc gccgcgcgcc aggtattccg cggcgatgag gtcggccgtg cgtgcgatcc
      661 cgagaccgct cgccgccgcg atcgtcggcg cctcggccgc gttgaactgc atcgcgaagt
      721 gcagcttgag ccgcttggag gtgtacggag gcgggaattg ccagtcggga ctgccgccgc
      781 ccggcggcgt cacgcccagc agccgatggt tgcgcaggtc gtcgggcgtg cgcggctcgc
      841 cgtgctcggc gagatacgcc ggcgacgcgc aggtgacgac gttgacctgg gcgatgcgtc
      901 gcgcgaccag cccgctctgc ttgagccctc cgacacgcaa ggcgacgtcg acgcgatctg
      961 cgacgacgtc gaccacgcgg tcgttgagac gcacgtcgag ttcgatggcg gggtagcggc
     1021 ggacgaactc gggcagtgcc ggcagcagca ggtagcgacc gaaggacacg ggcacgtcga
     1081 cacgcaaggg cccctggggc ttggagcgcg tgccccgcag cgtctcctcc gcgtcggcga
     1141 tctcgtcgag gatgcgtcgc gcacggcgca ggtagtccgt tccttcggcc gtgaggctga
     1201 cccggcgcgt cgtgcgattc agcagccttg ccccgaggtg cttttcgagt gccgcgacgt
     1261 gggcgctcgc ggcggcacgc gagatgtcga gttcgtccga ggcctgcgcg aatgatccgc
     1321 gctccgcgac ccgggtgaag actcgcatgg cgaccagttg atccacggct ggattattca
     1381 gctattcagg atggagtgca acgattcatc ctgtttataa aacgatctgc cgggtctatc
     1441 gttgcctccg acgattacca acggaggatc cagccatgac gaaccagaac aacctgcaga
     1501 accgactcct ctccggcgcg attgcggccc tggcggtgtc gatcctggtg gtctttgcct
     1561 ccctgacaca cacggtcgcg gccctggcca cccatgtctg aaaatgccgg cctacgaggg
     1621 tgggattggt tgctcaaatc caccctcgct ccagccgaat aaaggaagcc tctgaaaagt
     1681 ataataaaaa agcgttgcaa aaatgtgacg caagtcggat aaatttgctg cgacgcagca
     1741 tccatccggt ataatggcgc cgtcccagat ggactgaggc ggcgcccggc gggctgccca
     1801 aggcaccccc gccggtcgag gcctccagtc agatacagga ggcaccatgc acatccagga
     1861 acgcacctac tccgtgatcg ccatcgcggc cctcgcattg gccgcgagca tcccggtcat
     1921 cgtcctgctg gttcgcggga tcgccgcgct caggctcggc tgaccggctg cagggtgggg
     1981 gtgcccgaag tccagctcgg cgcacccctg tccccgaccg accacggccc cagccgtccg
     2041 cgctcgcaat gggccgttcc cgacgccggc gggcatcccg accaggtgac cttcaacgat
     2101 tgatcgaact cgcaggcaat actcccaggg cgtccgcgtg gaggctctgc acgtacaggt
     2161 caccctcccg ttccgtcacg ccgctcgtct ccggcaatcg gcctgaagga tcctgcaggt
     2221 ccgccaccac gcggccgtcc tcatcgaatg caaggacgtg cccgtaggcc ggcggcaccg
     2281 gccacagtga cttcggcagt cgcaggctca tcgcacgcag ccagggccgg tcggacagcc
     2341 ggtcgatggt ctcactgcgc ggcttggtca gcccggtcca caggcgaccg ttggtcccgc
     2401 gtgtcaggtt gtccgggaag cccgggaggt tcgccaggac gaccattgcc tgcgcattca
     2461 ccgggtcgcg ggcggccgcc ttggcgtcga gcgcgtccgc gtccacgtcc accttccaga
     2521 tgcggtactc gcccgtctcc gcgatgaaga gcttcgcctg gtcgcccgac aatgcaacgc
     2581 cgttcggaaa acaaagtccg tcgatgacca cccgcacttg ctgtgtggct ggctggtact
     2641 cgagcacgcg gcccgtgcac gagtgctcca tgacgtcgag cagcgccgcg tcgaacgtgc
     2701 cgaggtcgac gggtgagaag cgccgcgtgg cgtccgtgaa atacatgcgg ccgttgtcgg
     2761 ccaccaccac cgcgtccgca taggatatgg ggtccgaccc cacatgatcg gcgagtacgg
     2821 tgacggagcc gtccgcggca gccacgagca ggcccttcat cgcgtcggcg atgacgaggc
     2881 ggccatcccg gtcgaaatcc atgcccagcg ggcggccgcc ggtattcacg aacgtctcga
     2941 tgccggtgcc gtcgggattc atgcgcagga ccgcgccgga cagcaccccc gtgtacagct
     3001 tgccgtcggg cccgaagacg atgtgctccg gaccgacttc cggcgccacc gagacgaggc
     3061 gcgcgtcggc caggcgttcg ttacgggcgt agggaccggt gaatccttcc gagcgtggcg
     3121 cgtgccaggc cacgggctgg atcggtaccg gggaaaacag caggtaggcc gcgaccatca
     3181 gcgctgcgac caccagcacg atccgcacgg gtttcatcga ccccccctgt gtggcacgcc
     3241 acgagtgcca gcgacgatct tacgcgcgcg cttgcctgcg cgggagtcgc gcccatagca
     3301 tcgggcgcat ggacgaactg agactgaacc ctgcctggta tcgacccgag cacctcgact
     3361 tccgggagac cgtgcgccgc ttcgtggcgc gcgaggtact gccgcacgtc tcggactggg
     3421 acgaagccgg gagctttccg cgcgagctgt accggcgcgc cgcggagatc gggctgctgg
     3481 gcctgggatt ccccgaggag tacggcggga tcagtggtac cgatgtgttt catcggctgg
     3541 ttgcgagcat cgagctggcg cagtgcgggt ccggcgggct cgtcgcgtcg ctgatgtcgc
     3601 acacgatcgg cagcccgccg gtcgccttgc tcgggacgcc cgagcagaag gcgcgcgtcc
     3661 tgcccgcggt cctgtcgggg gggaagattg cggcgctcgc gatcaccgaa ccgtcgggcg
     3721 gctccgacgt cgcggcgttg cgaaccagcg ccgtgcgcga cggcgaccac tatgtcgtga
     3781 acggcgagaa gacgttcatc acgtcgggca tgcgtgccga ttacctgacc gtcgccgtga
     3841 ggaccagccc cgccggcagc ggtgccgggg ggatttcact cctgctcgtc gagggcgaca
     3901 cgccgggact ggagcggact gcgctgcgca agatgggatg gtgggcgtcc gacacggcac
     3961 acctgcgctt cacggactgc cgcgtccccg cgcgcaacct gctcggggcg gagggcgcgg
     4021 gctttcgctc gatcatgcag aacttcaatg ccgagcgctt cacgctcgcg gcgggcgccg
     4081 tcgggttcgc ctgggtctgc taccaggagg cgctggagtg ggcgcgcgag cgccggacgt
     4141 tcggccagcg gctcgccgac caccaggtcg tgcgccaccg cctggtcgac atgctgtcgc
     4201 gcatcgaagc ggcgcggacc atgctggagg aggcggcctg gcgcctcgag cacgcgccgg
     4261 ccggcgaccc cgggctggtg gcatcgttgt gcatgctgaa gaacgtcgcg acgcaggcga
     4321 tgcagtactg cgccgacgcg gccgtgcaga ccctcggcgg catgggttac atgcgcggca
     4381 cgcattccga gcggatctac cgggaggtca aggtcaacat gatcggcggc ggcaccgagg
     4441 aaatcatgaa ggagctggcg gcgcgccagt tggggctctg agcgcggtgg ccggggcatg
     4501 atggacgcag ggcccgtcgt cacgtaacgt caccaccaaa cgcaggcgag cggcatcgag
     4561 ccgccggtca cacaggggtc gagaaatgaa gcaactctcc gggatggacc acgtgttcct
     4621 gaagctggaa cacggcaacc agttcatgca cgtcgccggg ctcggcatct acgacccgtc
     4681 gacggcgccc ggcggcaagg tgcggttcaa ggacatcctg gcgttcttcg aggcgcgtct
     4741 ggactccgcg ccggtgttcc gccggcgtct cgtgagcgtg ccgatggacc tcgatcgccc
     4801 gtactggatc gaggacgacg acgtggacgt cgagttccac gtgcggcacg tcgcgctgcc
     4861 gcatccgggc gactggcggc agctgtgcat ccaggtggcg cgcatccatt cgcgtccgct
     4921 cgatcgcagc aagccgctgt gggaggcgta cgtcatcgag ggcctcgaca acattcccgg
     4981 catcccgcgc gggagcttcg cgttctacac gaagttccac cacgcggcga tcgacgggga
     5041 gggcggcacg gtcgtgttgc aggccatcca ctccttcgcc ccggacgagg ccgtgcccga
     5101 cgacgtgcgg cgcacgcgcg tccgcgaccg cgagccgacg cagctcgagc tctacacgcg
     5161 cgcaatcgtc aacaacctcc agcgcgtgcc gaaggcggcg agtttcacgt tgcagacggc
     5221 ccggcggctg gtgggtgccg gggcggggta catgggacag gtccgcgcga tgctccagga
     5281 agcgggtcta ccgtccgccg agacggtgaa gtccaggctg cgacggccgc cggtgacgcg
     5341 cttcagcggc aaggtctcgc cgcaccgggt cgtggaactc gtggcactgc cgatggccga
     5401 catcaagcag gtgcggcaga agatcgaggg cgccaccatc aacgacgtct tcatgacgac
     5461 cgtcggcggc gcgctcaatc gctatcttgc cgcgaaaggc gaattgccgg accgcaccat
     5521 gaccgcgcag gtgccgatga cgctgcgcgg cgagaacaag ggcggcgacc agggtaacca
     5581 ggtcggcgtg accgtgatgc cgctgcacac ggaggtcgcc gacccgatcg aacgcctgca
     5641 tgcgatcaag cagggagcgg cgaagtccaa ggcgctcgtc gcggtcgtgg gcaaggacct
     5701 cgcgaagaac ctctatgacc tgctgcccgc cgtggcgtcg gagatcttca cgacgcgggt
     5761 catgctgccg accatgaaca tcgtcgtgtc caacgtgcgc gggccggacg tgcccctcta
     5821 catggccggc gcgcagatgg tcgcgttcgc tccggtcagc atcgcgatga acgggctcgg
     5881 cctcaacgtc accggattca gctatcacgg cacgctgtgg atctgcgccg tcgcgtgccg
     5941 cgacatgatg cccgacccgg cttttttcgc ggactgcctg cgcaagagct tcgcggacct
     6001 cgcgacggcc gcgtcgcacg cgggtcacgc cgccaagggg gagcccagga cgaagacggg
     6061 gcgcgcaccc aagggcgcgc gccccgctcg caagcgctag ccgacgcggt cagttcgacg
     6121 gcgtcgtcgc cggcttcgcc gcgtccgcct tcgcgggctc ggggaagccg cgcttgcgca
     6181 tcagcgcccc cacgtccggg tcgcggccgc ggaactgccg gtaggcgtcg gccggctcga
     6241 ccgtgttgcc ctgcgacagc agcgccttga gcttcgcggc ctgcgccttg tcccaaggcc
     6301 cgccattctc gaggaaggac tcccacgcgt cggccgtgat cgtgtcggac cacaggtagc
     6361 tgtagtagcc ggccgaatag ccgtcacccg agaagatgtg caggaactgc ggcgtgcggt
     6421 gccgcatcac gatctcgctg ggcatgttga tctcggcgag cgtcgccttc tcgaactggt
     6481 cgggatcgat cgggcccgag ctcgccgcga ggtgcagctt catgtccacg atcgcactcg
     6541 acaggtactc gaccgtcttg aatccctggt tgaacttctc cgccttcttg atcttggcga
     6601 cgagctcggg agggatgggc ttgccggtct ggtagttgag cgcgtatcga ttgaggatct
     6661 ccggcgtcga gagccagtgc tcgagcagct gcgacgggaa ctcgacgtag tcgcgcgcga
     6721 cgttcgtgcc cgacagcgtc gggtagctca cgttggagct cagcccgtgc agcgcgtggc
     6781 cgaactcgtg gaacagcgtc gtcgcgtcct cccagctcac cagcaccggc tcgcccggct
     6841 tgcccttcac gaaattggag ttgttcgaga cgatggtgcg cacctcgccg tcgaagcgct
     6901 cctggttgcg gtacgcgttc atccacgcgc ccgactgctt gcccttgcgc gcgtacgggt
     6961 cccagtacca gagcccgacg aggccgccct tgtcgtccgt cacttcccag acgcggacgt
     7021 cggggtagta caccggcacc ttcttcgggt ccacgggcgt gaacctgaag ccgaacagtt
     7081 cccgcgccat gtagaacatc ccttcgcgca ggttctcgag ctgcaggtag ggcgtgatct
     7141 gggtctcgtc gaggtcgtac ttttccttgc ggaccttttc cgcgtagtag cggtagtccc
     7201 agggctcgat cttgatgccg gcgtgctcct tgtcggcaac cgcctgcatg tcggccactt
     7261 cctcgtgcac gcgcgcgacc gcggcgggcc acaccgccat catcagcccc atcgcgcgtt
     7321 cggggttctg cgccatcgag tcctcgaggc gccacgccgc gtgattggcg tacccgagca
     7381 gcttcgcgcg ctcgtcgcgc agcttgagga tctgggtgat ggtcgcgttg ttgtcggtgg
     7441 ctccgccttg gtcgccgcgg ctcacgaaca tgcgccagac cttctcgcgc agcgcacggt
     7501 tgtcggcata ggtcaggaat ggctcgacac tcgagcgggt gttgagtacc gcccactgtc
     7561 ccttcttgcc gcgcgatgcg gccgcttccg cttccgcgtc gcggatcgac gccggcaggc
     7621 cggcgaggtc cgcgggcttg tcgatgagca cgaccccgtt gttctcgtcg gccagcacgt
     7681 tctggctgaa cttcgtgtac aggccggcca gttcctggtt gatgtccgcc aggcgcttct
     7741 tcgccgccgc gtcgagcttg gcgccggcgc ggacgaagtt cgtgtagtac caccaggtca
     7801 gccgctgctg ctcggcggtc agtccggatt tctcgcgaac ctcgtagacc gcctcgatgc
     7861 gcttgaagag cgcctcgttc tgggtgatct ggtcctggaa cgcggcgagc ttcggcgcca
     7921 tttcgcgctc gacggcctgc accgcgtcgt cgttcatggt cgacgtgtat acgttgtaga
     7981 tggtggccgc gcgcgtgagc gcccggccgg tgcgctccag cgccgcgatc gtgttttcga
     8041 acgtcggcgg gttcgggttg gtcgcgatga ggtcgacctc cgcgagattg cggtccatcc
     8101 cgacctcgag cgcgggcccg atctcgtccg tcctgacctt gtcgaacggc ggcacgccac
     8161 cgtagggacc ggtccacggc gcgacgagcg gctgggcgag cttgagttgc gcctcggtgg
     8221 gctgcgtggc ggccgaggcg aaggtggctg cggcgagcgt ggccagtccg gcccacgcta
     8281 cgagttgcct tgggatcatg aatttcctca ctggatctgt gatttgtcgt cgggagtgaa
     8341 ttacctgcgc cgcccgccac ccatcggccg cggagcgggc gtcgggcgcg gcgccggccg
     8401 ggacatcccg gtcgacgggc gcggtgtcgg tgccgggcgg gcggctggct gagacgggcg
     8461 ctccgccggg agctgggtcg gcttccccgg cagcgtcgtc gggcgatccg gacgctcgcc
     8521 cggcggaacc tcgatcgggc ggccgtcgtc gtcgtaccag cagcacggat agcccgggta
     8581 gtagtagggg tagtagccgc ccgagtagta cccgtagctc acgccgacgc tcgcgtcgcc
     8641 gccgtacccg ccgtagccgt cgtccgatgc gcaacccgcg gcgagggcgc acacgacccc
     8701 accgaccgtc caccgcaaga cgcgaccagc gctcactgcc gcgcctcctg ggcgaccggc
     8761 ggccactcgg cgaccgcaaa tggcgcaccc gtcgggtcca gcatcacagc cacggtcgtg
     8821 ccgtgcatct tcgccggttg catgacgacc ttgcctccgg cagcgctcgc gcgcgcgacc
     8881 gtgtctccga cgtccttgac ctgcacgtag ggcaaccagg tcgtcgggac ctgggcaggc
     8941 cgcggcagga tgccggcgcg cgcgtagccg ccggaccaga gctggaatcc gtcgccttcc
     9001 ccgcgcgccg cacccgccac gacttcgtaa gggccgagcc cccggtagaa atccgccatc
     9061 ttcgccgcgt cggcggccca caactcggcc cacagccact ggtcgttgcc cgacaggaag
     9121 tcggccggat cgccggtcgc cgaggtgatc acgccgaaga tcccgccttc gggatccatg
     9181 agcagcgcct cggtgccgcg tccgccgacc ttgcgcggcg acacgacgac cttgccgccg
     9241 ccccgctcca cgagggcggc gctcgccgcg acgtcgccga ccgagatgaa cccgacccag
     9301 cgcgacgacg ggtatttttc gcgctggcgc gggcgcacgt agaccatccc gccgatgggt
     9361 gtcccgcccg acagcaccag cgtgtaggtc ttgaagtcgt ccttcggccc gtaggtctcg
     9421 aaggtccacc cgaagacctt gccgtaaaac tgcgcggccg tgccgacgtc gagcgtcagc
     9481 agttccaccc agacgaagcg gccggggtcg tggcgctgcg tcggctcatc ggtgatgggc
     9541 ggccagtatc gcgccggctc ggccgcccgg gtcggcgcgc cggccagcag ccccagcaat
     9601 gcaaccaatg caccaccaat cccaggcgtg atgcgcatgc ggctggctcc ttactcgcgc
     9661 cgatcgtgcc gggcccggca cgggctgcaa agtgtaatca attcgatcca gcggcgctgc
     9721 cgtcccgact gtcggcgcgt gccacgacgg cggcaaccag cgtgattgcc gcgaccgcgc
     9781 acaggatggc cagcatccgc gcgtagggca gccccatcgc ggcccggtgg gcggcgagca
     9841 cccaggccgt ccgcagccat tccgcggcac cgaggaacaa cgccacctgc agcacccgtc
     9901 gcgcccaggc gtcgtcgacg agcgacaagc cgatcagggc gatcgagacg gcgacgagcg
     9961 gatacagccc cgcgcgctgg aaatgcgctg cgagcagcag cagcgccacc gcgggcagcg
    10021 cgaccccgag gactcgcgac gtccccgggg cgctcacaag cgctcccaca tcaggtccga
    10081 gtcacgtgcg atcgcggtgc ggaccatgtc gagcagcggc acggcgcgct tctgcaaggt
    10141 gatgactggc tcgcgctcac gttcgtcttc atcgtcgccc gatggggatt gcgtggccgg
    10201 agagggagag ccgccggagg tctcgagcat gcgcaccagt cgcgcgaggg catcgggcaa
    10261 gtcggcggcg aggatcgcgc cagggacggt gccggtgtga cccatgccgc gcagcagcgt
    10321 caccgcagca tcgccgtgca tcgtcaactg cccgatacgc gtggaaaacc tgacgatcac
    10381 gccgcgccgc ccccgtgatt gggatcgtgc gatcataacg ctcgatttcc gcgcatacga
    10441 ggcttgtcgc atggagttcc agaacctggt ttgcgagcgc agggacggcg tgctggccgt
    10501 caccctcaac cggccgcaga aactgaacag cctgtcgctg gggctgctca aggacctgaa
    10561 gtcctgcgcg gagtcgatcc agcgggaccg caccgtgcgc gccgtgctgc tgaccggcgc
    10621 cggccgcggc ttctgcgccg gcgcggacct gaccgacccg gacagcattc cctcggcggg
    10681 gcagtccatg ggccagttgc tggcgtcgcg cctgcgcgat tactaccacc cggcggcgct
    10741 catgtggtcg aacctgccgg tgccgttggt ggtcgcattg aatggagtgg cggccggggc
    10801 cggtgcttcg ctggcgctct tcggcgacat cacggtcgcg gcgcaatccg ccaccctcac
    10861 gttcgtgttc gcaccaaagc tcggcctcgt tccggacatg ggtgcgacct acttcctcgc
    10921 acgcaggatc ggcgaggcgc gcgcgcggga ggtggcactt agcgggcgca agatcggggc
    10981 ggaggaggct gccaggatcg gcctgatcgc ggagtgcgtg gacgacgccc ggctgccgac
    11041 gcgcgcggaa gagatcgccc aggccctggc gtcgggccct cgcgaggcat tcgtcggggt
    11101 gaggcacctg atggcgatgg ccgcccgtgc ggggctggcc gagcagctcg aggcggaggc
    11161 cgctctgcag gcgaggctgg gcgacagccc cgacttcgtc gaaggcgtga cggcgttccg
    11221 ggagaagcgg gcgccgcgct tcggtgcggg ctgacggccc gccagcggcg cgaccggtca
    11281 ggagccggtc ttgtcggcca ggcggttcct gacgaagagc ttggtgcgca acgtcacgtc
    11341 gccgttgtgc cgctggtggg tcgcgatgtc gtaggagctg aacgagtcct cgagactcac
    11401 gtcgaggccg ggaccgcgca gccatcggcc gccgccgagc accacgtgca cgcactcgcg
    11461 ccaggtctcc ggcgggtcct tgagcgcgat gacgccgtcg gcgccggctg cgagggcctc
    11521 catcatgacc atcgtgtccg cttcgctcag cacggcgatg atcggatgcg tcgggaattt
    11581 ctcggtggcc agcgccagcg agtccatggt cccggtgcag tccaccagca gcccttcgcg
    11641 cgcgccgcgc tcgggcgagt cggtcagctt cacggcgccg ccgaggtagg cctcgagcag
    11701 cttgcggaat ggcgcgtacc cggagtacac gcggcacacc ggcgcggcgt cgcgccaggg
    11761 gtccacgagg gccatcccgt ctcgattgtt cacgaccggc ttgtccatcg ctcggctccg
    11821 actgtcattg gtggtgaatg tacctgtcat ttgcccggct cgaccgcaat atgtcgctcc
    11881 gatgtgnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntggt gaatgtacct
    12001 gtcatttgcc cggctcgacc gcaatatgtc gctccgatgt gacgaatctg cgggctcgtt
    12061 cacagcttgc gatgccgatt ggctacgatg tgcgcccgtg accgcctggg gcgcagaagg
    12121 catgacttca ggattcctcg cgatgcgcag gcatgcattg cgcgacgtac gcctggtcgc
    12181 tgccgcgctg cttgcgtgcg cagcgttgcc ggtcgccgcc gcgactccgg ccgcgacgga
    12241 gaccgcgccg aagccgcgta cgatgggcga gatcctcgat gcgtcgaagc cttccgactg
    12301 gcgcccgctc gttgcggaga acacgctgta cgtgcagctc gacagcgggc gcgtggtcat
    12361 cgagctcgcc ccggcattcg cgccgctgca cgccgccaac atccgcaagc tggtgcgtgc
    12421 cggctatttc gacggcctgc ccatcgtccg ttcccaggac aactacgtgg tccagtgggg
    12481 cgacccggac ggcaagcgca gcctcgggga cgccagcggg acattgccgc cggaattcac
    12541 cgtggcgtgg actcccgcgc tgccgttcac cgtcctgccc gacgtggacg gctatgcgcc
    12601 gcaggtcggc ttctccgacg gctttccggc ggggcgcgac ccgaagacgc ggcaggcgtg
    12661 gcttgcgcac tgctacggca tggtcggcgt cggtcgcggc aatgagctgg acagcggcaa
    12721 cgggtccgag ctgtacgtcg tcatcggcaa tgcgccgcga cacctcgatc gcaacatcgc
    12781 gctcgtgggt cgggtggtcg agggcatgga actgctgtcc acattgccac gaggcagcgg
    12841 gccgctcggg ttctacgaga agcccgagca gtacgtcccc attcgcagca tccgacttgc
    12901 cgccgacgtt cccgccgctg aacggccgaa cctcgaagtc attcgcaccg acacggcgac
    12961 cttcgaggca ctggtcgaga gccgacgcaa ccgtcgcgac gactggtaca aggtgcccgc
    13021 cggctacgtc gaactctgca acgtgccgat cgtcgtgcgg tcccggccct gattgctgcg
    13081 atgcagcagg atcgaggcag gcaagcggaa ccctatcggg ccgggcggag tcacatgccg
    13141 gggccctgac gcgacgccgc ttatgacatt gcggattgcc gcggggcccc cattgatgca
    13201 agttgtggtc gtggtgacga caggggaagg gacatggatc gattctggct gaagaactat
    13261 ccgcccggcg tgccggcgga cgtggacgtc acgcagtact cgtccctcgt ccagctcatg
    13321 gaggaggcat tcgccaagtt cggcaagcgg acggcctacg cgtacatgga ccggtacttc
    13381 acgttcgacg acgtagaccg ccactcgcgt gcactcgcgg cctggctgca ggcgcgcggg
    13441 ctcgcgcgcg gcgcgcgcgt tgcgatcatg atgccgaacg tcgtgcagta cccgatcgcg
    13501 gtggccgcga tcctgcgcgc gggctacgtg gtcgtgaacg tcaacccgct ctacacgccg
    13561 cgcgaactcg agcaccagct gaaggattcc ggtgccgagg cgatcgtgat cctcgagaac
    13621 ttcgcgacga cgctcgagca ggtcgtcgcg aagacgtcgg tcaagcacgt ggtcgtcgcc
    13681 acgatgggcg acatgctcgg cttcccgaag gggctgatcg tcaactacgt cgtgcgccgc
    13741 gtgaaaaaga tggtgccggc ctggtcgttg ccgggtcacg tgcggttcac ggcggcgctc
    13801 gacgcgggcg cgcggctcaa gctcgcaccg gtgcaggcga cgcgctcgga catcgcattc
    13861 ctgcagtaca ccggtggcac caccggcgtg tcgaagggcg cgacgctcac gcacggcaac
    13921 ctggtcgcca acgtgctgca gatggaggcg tgggtcgagc cggcgctcaa ggacacgagt
    13981 cgcgggccgg tgccggacca gctcgtctac atctgcgcgc tgccgctcta ccacgtgttc
    14041 gcgctcacgg tgaactgcct cggcggcctg cggctcggct cgatgaacgt gctgatcgtg
    14101 aacccgcgcg acatggcggg cttcgtcaag gaaatcgcca agtacaagtt ccacgtgatc
    14161 ccgggcgtga acacgctctt caacgggctc gcggaaaacg ccgagttccg caagctcgac
    14221 ttctcgagcc tcaggatcgc caacggcggc gggatggccg tgcagaaggc cgtctccgac
    14281 aagtggctcg cgctcacggg gattccgatc atcgaaggct acggcctgtc ggagacctcg
    14341 cccgtcgcca cgacgaaccc gatcacgagc cgggaatgga acgggacgat cgggctgccg
    14401 gtgccgaaca ccgagatcgc gatcctcgac gacgcgggac agccggtgcc gctcggacag
    14461 cccggcgaaa tcgccatccg cggaccgcag gtgatggcgg gctactggca gcgtccggac
    14521 gagaccgcga aggtcatgac gcccgacggg ttcttcaagt cgggcgacat cgggatcatg
    14581 gacgagcgcg gctacacgaa gatcgtggac cgcaagaagg acatgatcat cgtgtcgggg
    14641 ttcaacgtat acccgaacga gatcgaggcg gtgatcgcca cgcacccgaa ggtgctcgaa
    14701 tgcgcggcga tcggcgtcgc ggacgagcgt tccggcgagg ccgtcaaggt cttcatcgtg
    14761 cgcaaggacc cgtcgctcac cgaggcggag atcgcggagt tctgccgcga gcagctgacc
    14821 ggctacaaga agccgaagtt catcgagttc cgcaccgagc tgccgaagac gaacgtcggc
    14881 aagatcctgc ggcgcgcgct gcgcgaaccc gtggtgagcg aggtggccag gagcgcctga
    14941 cgcggcggag cccgtaacgt cgacatgtgt gccccggcga acgccggggc accgtgtcgc
    15001 accgccgctc cgtgccccgg gcagcccggc ccaatcgacg cggcgaatca gcgtcccgcg
    15061 atctggaaca agggattggc ctggcgcgac gcccgtcagc ccaagcgagg ttggccggtc
    15121 acctcctcac cattgtcgag gtacgcggtt ccgatccagc gcgtggaccc gcctgggggc
    15181 cgcgctggtc gctgaagtaa gacagcgtcc cgatcaccgt tccgaatcgg gacgccttgc
    15241 cctcgaagtt cacgtgatgg acgcctccgc tggaccgggg cgggaacggt tccgacaggc
    15301 caggccccgg ggtgcttcag ggcccggtcg ggcagccacc gcgcgggaat cgccagcgtc
    15361 gagcgattgc caacgtggcc acgaagctgc cgagcacggc cgccacgagc gggaattcga
    15421 tgatcagcac tagaggcgtg agcagcatac gcgccatgaa gcgcagcgac tcgtgccgag
    15481 ccacgaccg
//
LOCUS       DAOMFD010000033        15202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_859_length_15202_cov_34.501155, whole genome shotgun sequence.
ACCESSION   DAOMFD010000033 DAOMFD010000000
VERSION     DAOMFD010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_859_length_15202_cov_34.501155"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1391
                     /locus_tag="PL152_03470"
     CDS             <1..1391
                     /locus_tag="PL152_03470"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HQR48367.1"
                     /translation="TGLYLLDQLAFLFVGVPLLHRLHLLAIGVLTLAALTWMLARRKG
                     RTAVAVPPKQLAFIRATAWLALAGLVVALGANVIGNVSLAEALTGGILDTAYVGLAFY
                     AGATVLNAILKLLLARRGMTRFRVVTRHTGPLLQSLARLVNVVAFAAWVLVVLHEFRV
                     YRPVQQWAGAVLTYPLEAGEISITLGSVLMFLAAVWLAVWLARTVRVVLRDEVMPKMD
                     LPRGVANSVSTLTYYGIMTIGVVVALAAAGFHISQLAFIIGALGVGIGFGLQNVVNNF
                     VSGLILMFERPIQPGDVVEVGGVSGKVREIGMRATTLGTFEGADVVVPNGVLLNEKLV
                     NWTLSDTDRRIDVNLGVAYGSEPRQVLELLMKVVTTTPGIATVPEPTVLFAGFGASSL
                     DFSIRAWTNNFGDWVKIRSDLSVRVYDALKAAGIEIPFPQQDLHLRSVSDAARNQLAG
                     LGGTPSGAGPAG"
     gene            1391..2071
                     /locus_tag="PL152_03475"
     CDS             1391..2071
                     /locus_tag="PL152_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HQR48368.1"
                     /translation="MLVEPGLLSTGVSLACDVVAAAVVAIAALTAPWRIWLEDRERQW
                     VWLLSLALLVGIWSMKAGITPGLSVRFLLVTALTLMHGWQLAVIGAVLVLGVLDFAGQ
                     ASWEDFGANLLCMAIVPALVTAWFHELVHARLPHNYFIYFFVTVFLGAAIAYNLAGLA
                     RIALLVMSGSLEAAHAGPEYFAILPLMSFGEGTVNGMVMAMAVVYRPKWVMSFDDRLY
                     LRKRPGTG"
     gene            complement(2108..4507)
                     /gene="lon"
                     /locus_tag="PL152_03480"
     CDS             complement(2108..4507)
                     /gene="lon"
                     /locus_tag="PL152_03480"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HQR48369.1"
                     /translation="MSTNESERTKPGVSPGGPGLPEDMLIVLPVRNLVLFPGVVLPVA
                     VKRDKTVAGAQEALRTESKVGFLLQKSPETEDPGFDDLNRIGTAAAIIRFVTAADGTH
                     HLVCQGERRFRVLDHVPGFPYLVARVEYLPDSGAHHPEVEARTLFLKQKSVEAISLLP
                     QAPAELANAVQAMESPSALADMVASFLDVTPAQKQALLETTDVRDRLEAVSGHLTHRV
                     EVLKLSRQIEQQTKEAIDERQREILLREQMRQIQKELGDEGEASETAELGEAIGKAGM
                     PPDVEEQARKELKRLERMNEASGEYSMARTYLDTLIAMPWSKLDEESIDIERARGILD
                     ADHYGLDRIKKRIVEYLAVRKLNPQGRSPILCFVGPPGVGKTSLGQSIARALGLKFQR
                     TSLGGVHDEAEIRGHRRTYIGALPGTIAQALKKAGTRNPVLMLDEIDKLGRGIQGDPA
                     SALLEVLDPEQNSTFRDNYLGVPFDLSKVLFIATANQLDTIPGPLRDRMEIIELTGYT
                     EEEKVEIARRYLVRRQLEANGLKPEQAAISDDALRSIARDYTREAGCRSLEREIGAVL
                     RNVAVRIAEGKTATVRIDAADVTEILGAPKFENEVAMRVSVPGVATGLAWTPVGGDIL
                     FIEATRIPGKGALILTGQLGDVMKESAQAAMSLVKSQSESLGVDPAIFEKSDIHVHVP
                     AGAIPKDGPSAGVAMYTALVSLLTNRTVRNDVAMTGEISLRGLVLPVGGIKEKTVAAH
                     RAGIHKVLLPARNRKDLEDIPESVKREIEFVWVERVDEAVANAIGTPVSGPAPLAQTG
                     T"
     gene            4714..5334
                     /locus_tag="PL152_03485"
     CDS             4714..5334
                     /locus_tag="PL152_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR48370.1"
                     /translation="MTSAIRDRFGPEFYRRHYFDPRTRVVTRAEMARRAELVAAFVRH
                     AELDVRSILDVGCGLGLMRHHLQRRFPRARYVGLEASPYLCEKFGWECGSAATYDPGR
                     TFDLVVCYDVLQYLTSREAAAAIRNLARLCSGILHFGVLTREDWELYCDQRSTDRNVH
                     MRPGNWYRRRLAPAFINAGSGMFVRRGAPVHLWELDKVELLRSRRG"
     gene            5428..6459
                     /locus_tag="PL152_03490"
     CDS             5428..6459
                     /locus_tag="PL152_03490"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQR48371.1"
                     /translation="MTMRLMLIAGVVAAFAGGTSTAQTWEALPTVAPAPATNPTVAAR
                     VDLGRMLYHDPRFSETGTVSCSSCHNLMEGGDDHRPTSIGVHGMKGGRNAPTVFNAAF
                     LTSQFWDGRAPTLEAQAKGPIVNPVEMGMKDLGSAIDRIRKIPGYAPYFKAAFGDGDV
                     VTIDNAADAIAAFERTQITPNSPYDRYVQGDKSTLTAQQQRGMKAFAETGCQACHSGV
                     VFAGPALPMGTPFLQKFPLNATDPFVKQYDLLADKGRYNTTKNPADEYMWRVPQLRNL
                     IYTAPYLHNGSVKTIPEMVLLMGRVQLGKDVPPATVADITAFLESLTGEFPRELMPRL
                     PPTPGDLLQ"
     gene            6593..7195
                     /locus_tag="PL152_03495"
     CDS             6593..7195
                     /locus_tag="PL152_03495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48372.1"
                     /translation="MRPTPLRLILPALSLLALAGCATSSSSCGGDTDYLKAQERPRLD
                     LPPDVTASERIQPIVIPALAPDRQKLDPQPRCLDYPPQYFARKPAAAGSPEAAVRAWG
                     AAWAARKPDVVMQAYSPSFQSPAAGGSTAFLAEREEAVATGAAPSADLQDLTVASDGP
                     DRRIVTFTQVFGDQRVRRELTLVRDGQAWRIVAERTLGTP"
     gene            7236..9704
                     /locus_tag="PL152_03500"
     CDS             7236..9704
                     /locus_tag="PL152_03500"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HQR48373.1"
                     /translation="MRNTSLVSGVTLGPGTLRAVLLDRLTRSLGKHPDTATQRDIYEA
                     LSLAVREELAARWLATQRRVAQAGVKRICYLSVEYLLGRTLVNGLSSLDGDLVQEARD
                     VLAELGFDLDRVAEEEIDPGLGNGGLGRLAACYLDSLATLQYPAVGYGIRYDYGIFTQ
                     AIDEDGRQREIASSWLRQHNPWEIERDDARYVIRFGGRCVATPDPAGRTRYRWVDTHN
                     IWAAGYDLLIPGNRSPTVNHLRLWAGRAIAPFNVEVFNAGRHAEASIEAVDAKNVSRI
                     LYPDDTTPQGKELRFKQQYFFVSASLQDMLAQHLVEGRPLDALHESVAIQLNDTHPAL
                     AIPELLRLLVDEHEMEWEAAWDITRRVFSYTNHTLLPEALEVWPVGFFERILPRHLQI
                     IYLLNRGLLDEVSATSPHDNDRRQRMSLIDESHGRQVRMSHLAVIASHTTNGVARLHS
                     ELMKQTIFRDFADLYPDRFTNVTNGIAVRRWLKQSNPGLSALLTERLGSSWENDLEEL
                     ERLRDAADDPGFCARFAEVKLANKRRLAEVIQRRIGIEADPSSLFDVQVKRIHEYKRQ
                     LLNLLHVVTRYNRIRANPDAPVVPRTVIFAGKAAPGYTMAKAVIKLINNVASVVNNDR
                     VVGGRLRVAFLPDYDVSLAQRIMPAADLSEQISTAGMEASGTGNMKLALNGAITIGTL
                     DGANIEIREQVGAENIFIFGLTSDEVAAYRKRGYSPAEVVNGNAELRRTLEQIDSGFF
                     TPGNLADGKPVVDRLMSDGEPFFVLADYAAYCAAQDAVDRLYLQPAEWTRRAVLNTLG
                     MGIFSSDRSIREYSERIWRIKPVL"
     gene            9704..10825
                     /locus_tag="PL152_03505"
     CDS             9704..10825
                     /locus_tag="PL152_03505"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR48374.1"
                     /translation="MIRKALPVLAPLLLAACGQGGSGAGKAADVAVPVRVESVAAADV
                     PTIIEGVGNVVPITSVAIKARVDGQIERVAVRDGAEVRRGDVLFQLDRRPFQVALDAA
                     RANLERDEAQLAKSQDQLRRYQDVYTKGYVSTDQLEDVRANARSAAATVAADKAAIEN
                     ARLDLEFATLRSPIDGRVGRITLQVGNMVKALDAAPLATVNQMQPVYVEFAVPEQYLA
                     DVRKAAAQHDTTVELTAQGDAGAPIVRSGPLTFVDNAVDAPTGTIRLRATLQNDDGAL
                     WPGQFTRVRIRIPSEGPVLVVPSSAIGQSPNGPYVYVVTAEGRAELRPVVVARTDAEH
                     AVITRGVVAGERVVVDGQSRVVPGGKVSEVAGQPAKAGA"
     gene            10822..13893
                     /locus_tag="PL152_03510"
     CDS             10822..13893
                     /locus_tag="PL152_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR48375.1"
                     /translation="MNASELFIRRPVMTGLLTATLVVLGLASYLQLPVSDLPTVDFPT
                     ITVSARLPGASAETMAAAVATPLEKQLSTIASIDSMTSTSSQGSSQITVQFKLDRDID
                     GAAQDVQSAITAAARQLPPDMTTPPSFRKVNPADSPVYYVALSSDTLPMTVVDEYAET
                     TLAQRLSMLPGVAQVNVYGDQKYAVRVQVDPVRLASRGIGIDEVQEAIAQQSVNLPVG
                     QLQGATRMYTLDASGQFFNAAQFGGLVVAYRDGAPVHLRDVGRVLDSVQDDRSSARFN
                     GKPAVVLAVQRQPGANTVQLVDAVKAELARLEVGLPPGLQIQTVFDRSASIRASLHDV
                     QATLGLALVLVVLVIFLFLGSAKATGIPAVALPLSLLGTLPVMYLLGLGIDNLSLMAM
                     TLCVGFVVDDAVVMMENIARHVDMGKPPLAAALQGSREIGFTIVSMTLSLAAVFIPVL
                     FMGGIVGRLLHEFAVTIMVAVIVSGVVSLTLTPLLASRMLQEGTHGHRSPGTQWFGRL
                     YEATRRGYERSLDWALHHRRWVFGGFLLSVVASVALFWLVPKGFLPTDDTGQLYGLTE
                     GAQDISFQAMAGKQGELAQIIRRNPHVEGVMSFIGVGGSSQTLNNGRVLVRLKPAGTR
                     PSADAIARSLRSELMTVPGIRLYLSAPPVIRIGGTITKAQYLYVLQDSDTDRLYEWAP
                     KLEAALRGLPQLQDVTSDLQISSPRVALEIDRGRAAELGITASQIENALYSAYGQRQV
                     GTIYTPTNQYWVILEVAPEFRDSPAALGTLYVRGANDNVVPLSQVTRATNDVAPLVVT
                     HSGQVPSVNLSFNLAPGVSLSQAIAAIERAERGLQLPASIVVSFQGTAQAFQDSLSGL
                     GLLLAFALFVIYIVLGVLYESYVHPLTILSGLPAAALGALLTLLVFGRELDLYGFVGI
                     IMLIGIVKKNAIMMIDFALEAGRTGGRTPAEAIREACLVRFRPIMMTTMAALLGTLPI
                     ALSIGASGASRQSLGLAVVGGLLLSQFLTLYLTPVVYLYLNRFERRSPPPG"
     gene            13903..>15202
                     /gene="glgB"
                     /locus_tag="PL152_03515"
     CDS             13903..>15202
                     /gene="glgB"
                     /locus_tag="PL152_03515"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003844 - 1,4-alpha-glucan branching
                     enzyme activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HQR48376.1"
                     /translation="MPSRQKLTVSDIEALVQARHPEPRSLLGYHEYARKGGEPACMVR
                     ALEPAAEHVEVIWDDASAPSTLKRIHDAGLFEGRVPYRRPLSPYRLRIRYRDGVEHVR
                     HDAYYFSPQITDFDLYLFGEGNHHRIYLKLGAHPIELDGTPGTRFAVWAPNAERVSVV
                     GSFNLWDGRRHAMQMRGSSGIWELFVPDVGPGTTYKYEIRTPDGRTILKSDPFGFAMQ
                     LRPDNCSVVASLEGHDWGDQAWMEARGRARHPALPINIYELHPGSWRQRRDRQPPFPS
                     WRELADELIPYVRDLGYTHVELMGVAEHPYDRSWGYQVSGYYAPTARYGSPQDFMHFV
                     DRCHQAGLGVILDWVPAHFPRDEHGLAGFDGTALYEHADPRLGEHADWGTKIFNYGRH
                     EVRNFLVANALYWLDRYHVDGLRVDAVASMLYLDYSRRPGE"
BASE COUNT         2271 a         5341 c         5160 g         2430 t
ORIGIN      
        1 tcacggggct ctacctgctc gaccagctgg cattcctgtt cgtgggcgtg ccgctcctgc
       61 accgcctgca cctgctcgcg atcggcgtgc tcaccctggc tgcactcacg tggatgctgg
      121 cgcgtcgcaa gggccgcacg gctgtcgcag tgcctccgaa gcaactcgcc ttcatccggg
      181 cgacggcgtg gctcgcgctc gcgggcctcg tcgtcgcgct cggggcaaac gtcatcggca
      241 acgtatcgct ggccgaggcg ctcaccggcg gcattctcga caccgcgtat gtcggccttg
      301 cgttctatgc cggcgccacg gtgctgaatg cgatcctgaa gctgctgctg gcacgacgcg
      361 gcatgacccg cttccgcgtc gtgacacggc acacgggtcc gctgctgcag agcctcgcgc
      421 gcctggtgaa tgtcgtcgcc tttgctgcgt gggtgctcgt cgtcctgcac gagttccgcg
      481 tgtatcgacc ggtacagcag tgggcaggcg ccgtgctgac gtatccgctc gaggccggcg
      541 agatctcgat cacgctcggc agcgtgctca tgttcctggc tgcggtctgg ctggcggtgt
      601 ggctcgcgcg gacggtccgc gtcgtgctgc gcgacgaggt catgccgaag atggacctgc
      661 cgcgcggcgt ggccaacagc gtttcgacgc tcacctacta cggcatcatg acgatcggcg
      721 tcgtggtcgc gctcgcggcg gccggattcc acatcagcca gctggcgttc atcatcggcg
      781 cgctcggcgt cggcatcggc ttcggcctgc agaacgtggt caacaatttc gtgtcgggcc
      841 tgatcctgat gttcgagcgg ccgatccagc ccggcgacgt ggtcgaggtc ggcggtgtct
      901 cgggcaaggt ccgcgagatc ggcatgcgcg ccaccacgct cggcacgttc gagggcgcgg
      961 acgtcgtcgt gccgaatggc gtgctgctca acgagaagct ggtcaactgg acgctgagcg
     1021 acaccgaccg gcgcatcgac gtgaacctcg gcgtcgcgta cggcagcgaa ccgcgacagg
     1081 tgctcgagct gctgatgaag gtggtcacca cgacgccggg gatcgcaaca gttcctgagc
     1141 cgacggtgct ctttgctgga ttcggcgcga gctcgctcga cttctcgatc cgtgcctgga
     1201 ccaacaactt cggcgactgg gtcaagatcc gcagcgacct gtcggtgcgc gtgtacgacg
     1261 cgctcaaggc ggcgggcatc gagatcccgt tcccgcagca ggacctgcac ctgcgcagcg
     1321 tgtcggatgc ggcgcgcaac caactcgccg gccttggcgg gacaccgtcc ggagccggac
     1381 cggcgggctg atgctggtcg agccgggact gctcagcacg ggcgtcagcc tcgcatgcga
     1441 cgtggtggcc gcggccgtcg tcgcgatcgc cgcgctgacc gctccgtggc gcatctggct
     1501 cgaggatcgc gagcgccaat gggtgtggct cctgagtctc gcgctgctcg tggggatctg
     1561 gtcgatgaag gccggcatta cgcccggact gtccgtgcga ttcctgctcg tcacggcgct
     1621 tacgctgatg cacggctggc agctcgcggt gatcggcgcg gtcctcgtcc tgggcgtgct
     1681 cgacttcgcc gggcaggcat cctgggaaga cttcggcgct aatctcctct gcatggccat
     1741 cgtgccggcc ctggtgacgg cgtggttcca cgagctggtc cacgcgcgcc tgccgcacaa
     1801 ctatttcatc tacttcttcg tcaccgtgtt cctcggtgcg gccatcgcgt acaacctcgc
     1861 cggccttgcg cgcatcgcgc tgctcgtcat gagcggctcg ctcgaggcgg cccacgccgg
     1921 gcccgagtac ttcgccatcc tgccgctgat gagctttggc gaggggactg tgaacggcat
     1981 ggtcatggcg atggccgtgg tttaccggcc gaagtgggtg atgagcttcg acgaccggct
     2041 gtacctgcgg aagcgacccg ggacgggcta ggtcccaagg tcgggccgcc ggaaaatcgg
     2101 cctggcgtca ggtcccggtc tgggcgagcg gcgcgggacc ggagaccggc gtgccgatcg
     2161 cattcgccac ggcctcatcc acccgctcga cccacacgaa ctcgatctcg cgcttgacgc
     2221 tttcggggat gtcctcgagg tccttgcggt tgcgcgccgg cagcaacacc ttgtggatgc
     2281 ccgcccgatg cgccgcgacg gtcttctcct tgatcccgcc caccggcagt accagcccgc
     2341 gcaggctgat ctcgccggtc atcgcgacgt cgttgcgcac agtccggttc gtaagcaggg
     2401 agacgagggc cgtgtacatc gcgacgcctg cgctcggacc gtccttcggg atcgcacccg
     2461 ccggcacgtg cacgtggatg tcgctctttt cgaagatcgc aggatcgacg cctagcgact
     2521 cggactgtga cttcaccagc gacatcgccg cctgtgcgct ctccttcatc acgtcgccga
     2581 gctggccggt gaggatcagg gcgcccttgc cggggattcg cgtcgcctcg atgaacagga
     2641 tgtcgccgcc gacaggcgtc cacgcgaggc ccgtcgccac gcccgggacg ctcacgcgca
     2701 tcgcgacctc gttctcgaac ttcggcgcgc cgaggatctc ggtcacgtcg gccgcgtcga
     2761 tgcgcaccgt ggcggtcttg ccctcggcga tgcgcaccgc gacgttgcgc agcacggcac
     2821 cgatctcgcg ctcgaggctg cggcagccgg cctcacgtgt gtagtcgcgg gcgatactgc
     2881 gcagtgcatc gtcggaaatg gctgcctgct cgggcttgag cccgttggcc tcgagctgac
     2941 gacgcacaag gtagcggcgc gcgatctcga ctttctcctc ctcggtgtag cccgtgagct
     3001 cgatgatctc catgcggtcg cgcagcggcc ccgggatggt gtcgagctga ttcgccgtgg
     3061 cgatgaacag caccttcgac aggtcgaacg gcacgccgag gtagttgtcg cggaaggtcg
     3121 agttctgctc cgggtccagc acctcgagca gcgcggacgc aggatcgccc tggatgccgc
     3181 gcccgagctt gtcgatctcg tcgagcatca gcaccgggtt gcgcgtgccg gccttcttca
     3241 gcgcctgggc gatggtcccc ggcagggcac cgatgtaggt gcgccgatgg ccgcgaatct
     3301 ctgcctcgtc gtgcacgccg ccgaggctcg tgcgctggaa cttgagcccg agcgcgcgcg
     3361 cgatggactg gcccagcgag gtcttgccga cccccggcgg accgacgaag cacaggatcg
     3421 ggctgcggcc ctgcggattg agcttgcgca ccgcgaggta ctcgacgatg cgcttcttga
     3481 tccggtcgag gccgtagtgg tcggcgtcga ggatgccgcg cgcccgctcg atgtcgatcg
     3541 actcctcgtc gagcttcgac cacggcatgg cgatgagcgt gtcgaggtag gtgcgcgcca
     3601 tcgagtactc gccgctcgcc tcgttcatgc gctcgaggcg cttcagttcc ttgcgcgcct
     3661 gctcctcgac gtcgggcggc atgcccgcct tgccgatcgc ctcgccgagc tcggccgtct
     3721 cgctcgcttc gccctcgtcg ccgagctcct tctggatctg ccgcatctgc tcgcgcagca
     3781 ggatctcgcg ctgccgctcg tcgatcgcct ccttggtctg ctgctcgatc tgccgcgaaa
     3841 gcttcagcac ctcgacccgg tgggtgaggt ggcccgacac ggcctcgagc cggtcgcgga
     3901 cgtcggtggt ctcgagcaac gcctgcttct gcgccggcgt gacgtcgagg aagctcgcga
     3961 ccatgtccgc gagcgcggag ggggactcca tggcctgcac ggcattggcg agctccgccg
     4021 gcgcctgggg cagcagcgaa atcgcctcca cggacttctg cttcaggaac agcgtccgcg
     4081 cctcgacctc cgggtggtgc gccccggagt cgggcaggta ctcgacgcga gccacgaggt
     4141 aggggaatcc ggggacgtgg tcgagcaccc ggaaccgtcg ctcgccctgg cagacgaggt
     4201 gatgggtgcc gtcggcggcc gtcacgaagc ggatgatggc tgcggccgtg ccgattcggt
     4261 tcaggtcgtc gaacccggga tcctcggtct cggggctctt ctgcaacagg aacccgacct
     4321 tgctctcggt ccgcagggct tcctgcgccc cggcgaccgt cttgtcgcgc ttgaccgcga
     4381 ccggcagcac gacgcccggg aacagcacga ggttgcgcac cggcaggacg atcagcatgt
     4441 cttcgggcag gcccgggccg cccggtgaca cgcccggctt ggtacgctcg ctctcgttcg
     4501 tgctcatgta atgcacccct gccgggacag tgcccgggat catggacccg acgttggggg
     4561 cgcaagacgc agtctcaatg ccacgcgtcg ggcgggtgcc aggcctggag tcgcacgggt
     4621 gctttcgggc agtcgctcat taagcaggtg tcgtttataa agctggtctc acttggtgaa
     4681 ttcatggtca gacaattcag ataaactttt tggatgacat ccgcgatccg cgaccgcttc
     4741 ggccccgagt tctaccggcg gcactacttc gatccacgca cgagggtcgt cacgcgggcc
     4801 gaaatggcgc ggcgggccga actcgtcgcg gccttcgtgc ggcacgcgga actcgacgtc
     4861 cgctcgatcc tggatgtcgg gtgcggactg gggctcatga ggcaccacct gcaacggcgg
     4921 ttcccccggg cccgctacgt cggactcgag gcgagcccgt acctgtgcga gaagttcggc
     4981 tgggaatgcg gctcggcggc gacctatgac cccgggcgga cgttcgacct cgtcgtctgc
     5041 tacgacgtgc tccagtacct gacgtcccgg gaggcggccg cggcgatccg caacctcgcg
     5101 cgcctgtgct ccggcatcct gcacttcggc gtcctgaccc gcgaggactg ggaactctac
     5161 tgcgaccagc gcagcaccga ccgcaacgtc cacatgcggc ccggcaactg gtaccggcgg
     5221 cgcctcgccc cggcattcat caatgcggga tccgggatgt tcgtgcgccg cggcgcgccg
     5281 gtccacctct gggaactcga caaggtggag ctcctgcgct cccgtcgcgg ctaggcattc
     5341 ctcacggctt gacgatgcat ccgcgcaatt gccgtgatgc agcatgtggg cgaaacatcg
     5401 cggacagtca cccatggagg actgtccatg acgatgcgcc tgatgctgat cgccggagtg
     5461 gtcgccgcat ttgccggcgg cacgtcgacc gcccagacct gggaggcgct gccgaccgtg
     5521 gcgcccgccc ccgcgaccaa tccgaccgtg gccgcgcgcg tggatctcgg caggatgctg
     5581 taccacgatc cccggttctc ggagaccggc acggtttcct gctcgagttg ccacaacctc
     5641 atggagggcg gggacgacca tcggccgacc agcatcggcg tccacggcat gaagggcggc
     5701 cgcaacgcgc cgacggtgtt caacgccgca ttcctcacgt cgcagttctg ggacggccgg
     5761 gcgccgaccc tcgaagcgca ggccaagggg ccgatcgtga acccggtcga gatgggcatg
     5821 aaggacctgg ggtcggcgat cgaccggatc cggaagatcc cgggctatgc gccgtacttc
     5881 aaggcggcgt tcggcgacgg ggacgtggtc acgatcgaca acgccgccga cgccatcgcg
     5941 gccttcgagc gtacccagat caccccgaac agcccctacg accgctacgt ccagggcgac
     6001 aagtcgaccc tcacggcgca gcagcagagg ggcatgaagg cgttcgccga gaccggctgc
     6061 caggcctgtc actccggcgt cgtcttcgcc gggcccgcgc tgccgatggg cacgccgttc
     6121 ctgcagaagt tcccgctgaa tgcgaccgac ccgttcgtca agcagtacga cctgcttgcc
     6181 gacaagggtc gctacaacac gaccaaaaac ccggccgacg agtacatgtg gcgcgtgccg
     6241 cagctgcgga acctcattta cacggccccc tacctgcaca acggcagcgt caagaccatc
     6301 cccgaaatgg tgctgctgat gggccgggtg cagctcggca aggacgtccc gcccgccacg
     6361 gtcgcggaca tcacggcgtt cctcgagagc ctgacgggcg agttccccag ggaactcatg
     6421 cccaggctgc cgccgacgcc gggagacctg ctgcagtagg cgcaagaagg gggccagacc
     6481 ccaattcagc aagcgggtgc tgaaacgggg tctgaccccg gttgcgggga cccgcagcgg
     6541 gtggccgggc ctgtacaatg cccgcctcct gcatggcccc ccggtagtac cgatgcgccc
     6601 gaccccgctg cggctgatcc tgcccgcgct ttcgctcctt gcgctcgccg ggtgcgcgac
     6661 ctcgtcgtcg tcctgcgggg gcgacaccga ttacctcaag gcccaggaac gcccgcgcct
     6721 cgatctgcca ccggacgtga cggccagcga gcgcatccag ccgatcgtga tcccggcgct
     6781 tgcgcccgac cggcagaagc tcgacccgca gccgcgctgc ctcgactacc cgccgcagta
     6841 tttcgcccgc aagcccgcgg ccgcgggctc gccggaggcc gccgtgcggg cctggggcgc
     6901 ggcctgggcg gcccgcaagc cggacgtggt gatgcaggcc tactcgccgt cgttccagtc
     6961 gcccgcggcc ggcggttcga cggcgttcct cgccgagcgc gaggaagcgg tggcgaccgg
     7021 cgccgctcct tcggccgacc tccaggacct gacggttgcc agcgacggcc ccgaccggcg
     7081 catcgtgacg ttcacgcagg tgttcggtga ccagcgcgtc cgccgggagt tgacgctcgt
     7141 gcgcgacggc caggcctggc gcatcgtcgc ggagcgcacc ctcgggacgc cctgagcgcg
     7201 ggccgccgcg cgcccggtcc acggacccag cgaccgtgag aaacacttcc ctcgtcagtg
     7261 gcgtcaccct cgggcccggc acgctccgtg cggtgctgct cgaccggctg acgcgatcgc
     7321 tcggcaagca cccggatacc gcgacccagc gggacatcta cgaagcactg tcgctcgcgg
     7381 tgcgcgagga actcgcggcg cgctggctcg ccacgcagcg gcgcgtcgcg caggcgggcg
     7441 tgaagcgcat ctgctacctg tcggtcgaat acctgctcgg gcgcacgctg gtgaacgggc
     7501 tgtcgtcgct cgacggcgac ctcgtgcagg aagcgcgcga cgtgctcgcc gaactcgggt
     7561 tcgacctcga tcgcgtggcc gaggaggaaa tcgacccggg actcggcaat ggcggcctcg
     7621 gccggctcgc ggcctgctac ctcgactcgc tggccacgct gcagtacccg gcggtcggct
     7681 acggcatccg ctacgactac ggcatcttca cgcaggccat cgacgaggac ggccgccagc
     7741 gcgagatcgc gtcgagctgg ctcaggcagc acaacccgtg ggagatcgag cgcgacgacg
     7801 cgcgctacgt gatccgcttc ggcggccggt gcgtggccac acccgacccg gcgggccgca
     7861 cccgctaccg ctgggtggac acgcacaaca tctgggccgc gggctacgac ctgctcatcc
     7921 ccggcaaccg cagccccacg gtgaatcacc tgcgcctgtg ggcgggccgg gcgatcgcgc
     7981 cgttcaacgt cgaggtgttc aacgccggcc ggcacgccga ggcctcgatc gaggcggtcg
     8041 acgccaagaa cgtgtcgcgc atcctctacc cggacgacac cacgccgcag ggcaaggaac
     8101 tgcgcttcaa gcagcagtac ttcttcgtga gcgcgagcct gcaggacatg ctggcgcagc
     8161 acctcgtcga gggccgcccg ctcgacgcgc tgcacgagtc ggtcgcgatc cagctgaacg
     8221 acacgcaccc ggcgctcgcc atcccggaac tgctgcgcct gctcgtcgac gagcacgaga
     8281 tggagtggga agcggcctgg gacatcaccc ggcgcgtgtt ctcctacacc aaccacacgc
     8341 tgctgccgga agcgctcgaa gtctggccgg tgggattctt cgagcgcatc ctgccgcgcc
     8401 acctgcagat catctacctg ctgaaccgcg gcctgctcga cgaggtctcc gccacgagcc
     8461 cgcacgacaa cgaccgccgc cagcgcatgt cgctgatcga cgagtcgcac ggccggcagg
     8521 tgcgcatgtc gcacctcgcg gtgatcgcga gccacacgac gaacggcgtg gccaggctcc
     8581 actcggagct catgaagcag acgatcttca gggatttcgc cgacctctac ccggatcgat
     8641 tcaccaacgt gaccaacggc attgcggtcc gccgttggct caagcagtcg aacccgggcc
     8701 tctccgcgct gctcacggag cggctcgggt cgtcctggga gaacgacctc gaggaactcg
     8761 agcgcctgcg cgacgccgcg gacgacccgg ggttctgcgc gcgcttcgcc gaggtgaagc
     8821 tcgccaacaa gcggcggctc gccgaggtga tccagcgccg catcggcatc gaggccgacc
     8881 cctcatcgct gttcgacgtg caggtgaagc gcatccacga gtacaagcgc cagctgctca
     8941 acctcctgca cgtcgtgacg cgctacaacc gcatccgcgc caatccggac gcgccggtcg
     9001 tgccacgcac ggtgatcttc gcgggcaagg ccgcacccgg ctacacgatg gcgaaggccg
     9061 tcatcaagct gatcaacaac gtggcgagcg tggtgaacaa cgaccgggtc gtcggcggac
     9121 ggctccgcgt ggcgtttttg cccgactacg acgtctcgct cgcgcagcgg atcatgccgg
     9181 cagcggatct gtccgagcag atttcaacgg ccggcatgga ggcttcgggc acgggcaaca
     9241 tgaagctcgc gctgaacggc gccatcacga tcggcacgct cgacggcgcc aacatcgaga
     9301 tccgcgagca ggtgggcgcc gagaacatct tcatcttcgg gctcacctcc gacgaggtcg
     9361 cggcctaccg caagcgcggc tactcgcccg ccgaggtcgt gaacggcaac gccgagctgc
     9421 gccgcacgct cgagcagatc gactcgggat tcttcacgcc gggcaacctc gccgacggca
     9481 agccggtcgt cgaccggctg atgagcgatg gcgagccgtt cttcgtgctg gcggactacg
     9541 ctgcgtactg tgccgcgcag gacgcggtcg accggctgta cctgcagccg gccgagtgga
     9601 cgcggcgcgc ggtgctcaac acgctcggca tgggcatctt ctcgagcgac cgcagcatcc
     9661 gcgagtactc ggaacgcatc tggcggatca agccggtcct gtgatgatcc gcaaggcgct
     9721 gcccgtactg gctccgttgc tgctggcagc ctgcggccag ggcggcagcg gtgccggcaa
     9781 ggcggcggac gtcgccgtcc ccgtacgggt cgagtccgtc gccgcggccg atgttcccac
     9841 catcatcgag ggcgtgggca acgtcgtgcc gatcaccagc gtggcgatca aggcccgcgt
     9901 ggacgggcag atcgaacgcg tcgcggtgcg tgacggggcc gaggtgcgcc gcggcgacgt
     9961 gctgttccag ctcgaccgac ggccgttcca ggtggcgctc gacgccgcgc gcgccaacct
    10021 cgagcgcgac gaagcccagc tcgcgaagag ccaggaccag ctgcgccgct accaggacgt
    10081 gtacaccaag ggctacgtgt ccaccgacca gctcgaggac gtccgcgcca atgcccgctc
    10141 cgcggccgcg accgtggcgg ccgacaaggc ggcgatcgag aacgcgcgcc tcgacctgga
    10201 gttcgccacg ctgcgctcgc ccatcgacgg ccgcgtgggg cgcatcacgc tgcaggtcgg
    10261 caacatggtg aaggcgctcg atgcggcgcc gctcgcgacg gtcaaccaga tgcagccggt
    10321 gtacgtggag ttcgcggtgc cggagcagta cctcgccgac gtccgcaagg ccgccgcgca
    10381 gcacgacacg acggtcgagc tgaccgcgca gggcgatgcc ggcgcgccga tcgtgcgcag
    10441 cggcccgctc accttcgtcg acaacgcggt ggacgcgccg actggaacca ttcgcctgcg
    10501 tgccacgctg cagaacgacg atggcgcgct gtggcccggc cagttcacgc gcgtcaggat
    10561 ccgcatcccg tcggaagggc cggtgctcgt cgtgccgtcg agcgcgatcg ggcagagccc
    10621 gaacgggccg tacgtgtacg tcgtcacggc cgagggccgt gccgagctgc ggcccgtggt
    10681 cgtcgcgcgc accgacgcgg aacacgccgt catcacgcgc ggcgtcgtgg cgggcgagcg
    10741 cgtcgtcgtg gacggccagt cgcgcgtggt gcccggcggc aaggtcagcg aggtcgccgg
    10801 gcagcccgcc aaggccgggg cgtgaacgcg agcgagctgt tcatccggcg gcccgtgatg
    10861 accgggctgc tcacggccac gctcgtcgtg ctcggactcg cgtcctacct gcagctgccg
    10921 gtctccgacc tgccgaccgt cgatttcccg accatcaccg tctcggcgag gctgccgggc
    10981 gccagcgccg agacgatggc ggccgcagtt gccacgccgc tcgagaagca gctgtcgacc
    11041 atcgcgtcga tcgactcgat gacgtcgacg agttcgcagg gcagcagcca gatcacggtg
    11101 cagttcaagc tcgaccgcga catcgacggc gcggcgcagg acgtgcagtc ggccatcacg
    11161 gccgcggcgc gtcagctgcc acccgacatg acgacgccgc cgtcgttccg caaggtgaac
    11221 ccggccgact cgcccgtgta ctacgtcgcg ctcagctccg acacgctgcc gatgaccgtc
    11281 gtcgacgaat acgccgagac gaccctcgcg cagcgcctgt ccatgctgcc cggcgtggcg
    11341 caggtcaacg tgtacggcga ccagaagtac gccgtgcggg tgcaggtgga cccggtccgg
    11401 ctcgcctcgc gcgggatcgg catcgacgag gtgcaggagg cgatcgccca gcagagcgtc
    11461 aacctgccgg tgggacagct gcagggcgcc acgcgcatgt acaccctcga cgcgagcggc
    11521 cagttcttca atgccgcgca gttcggcggg ctggtcgtcg cgtaccgcga cggcgcgccc
    11581 gtgcacctgc gcgacgtcgg ccgcgtcctc gacagcgtgc aggacgaccg ctcgagcgcg
    11641 cgcttcaacg gcaagccggc ggtggtgctg gcggtgcagc gccagccggg cgcgaacacc
    11701 gtgcagctgg tcgacgcggt caaggcggaa ctcgcgcggc tggaggtggg cctgccgccg
    11761 gggttgcaga tacagacggt cttcgaccgg tcggcctcga tccgggcctc gctgcacgac
    11821 gtgcaggcga cgctcgggct cgcgctcgtg ctggtggtgc tggtgatctt cctgttcctc
    11881 ggcagcgcga aggccaccgg catccccgcg gtcgcgctgc cgctctcgct gctcggcacg
    11941 ttgccggtga tgtacctgct cgggctcggc atcgacaacc tgtcgctcat ggcgatgacg
    12001 ctgtgcgtcg ggttcgtcgt cgacgacgcc gtggtgatga tggagaacat cgcgcggcac
    12061 gtcgacatgg gcaagccgcc gctcgcggcc gcgctccagg gttcgcgcga gatcggcttc
    12121 acgatcgtgt cgatgacgct gtcgctcgcc gcggtgttca ttccggtgct gttcatgggc
    12181 ggcatcgtcg gccggctgct gcacgagttc gccgtgacca tcatggtggc ggtgatcgtc
    12241 tccggcgtcg tctcgctcac gctcacgccg ttgctcgcgt cgcgcatgct gcaggaggga
    12301 actcacgggc atcgcagccc cgggacgcaa tggttcgggc gcctgtacga ggcgacccgg
    12361 cgtggctacg agcgcagcct cgactgggca ctgcaccacc ggcgctgggt gttcggcggc
    12421 ttcctgctgt cggtggtcgc gtcggtcgcg ttgttctggc tcgtgcccaa gggcttcctg
    12481 ccgaccgacg acaccggcca gctctacggt ctcaccgagg gagcgcagga catctcgttc
    12541 caggcgatgg ccgggaagca gggcgaactc gcgcagatca tccgccgcaa cccgcacgtc
    12601 gagggcgtga tgtcgttcat cggcgtgggc gggtcgtcgc agacactcaa caacggtcgc
    12661 gtgctggtgc gcctcaagcc cgctggcacg cggccgagcg ccgacgcgat cgccaggtcc
    12721 ctgcgctccg agctcatgac ggtgccgggc atccgcctgt acctgtcggc cccgccggtc
    12781 atccgcatcg gcggcaccat caccaaggct cagtacctgt acgtgctgca ggactccgac
    12841 acggaccgcc tgtacgaatg ggcgccgaag ctcgaggcgg cgctgcgcgg acttccgcaa
    12901 ctgcaggacg tcacgagcga cctgcagatc agttcgccga gggtggccct ggagatcgac
    12961 cgcgggcgcg cggcggaact cggcatcacg gcgagccaga tcgagaacgc cctctacagc
    13021 gcgtacggcc agcgtcaggt cggcacgatc tacacgccga ccaaccagta ctgggtcatc
    13081 ctggaggtgg cgccggagtt ccgcgactcg ccggcggcgc tcggcacgct gtacgtccgg
    13141 ggcgcgaacg acaacgtcgt gccgctctcg caggtcacgc gcgccacgaa cgacgtcgcg
    13201 ccgctggtcg tgacgcactc cgggcaggtc ccgtcggtga acctgtcgtt caacctcgcg
    13261 cccggcgtgt cgctgagcca ggcgatcgcg gccatcgagc gtgcggaacg cgggctgcaa
    13321 cttccggcat cgatcgtcgt ctctttccag ggcaccgccc aggcgttcca ggactcgctg
    13381 tcggggctcg ggctgctgct cgcattcgcg ctgttcgtca tctacatcgt gctcggcgtg
    13441 ctctacgaga gctacgtgca cccgctcacc atcctctccg gactgcccgc ggcggcgctc
    13501 ggcgcgctcc tcacgctgct cgtgttcggg cgcgagctcg acctctatgg tttcgtcggc
    13561 atcatcatgc tgatcggcat cgtgaagaag aatgcgatca tgatgatcga cttcgccctg
    13621 gaggccgggc gcaccggcgg gcggacgccg gcggaggcga tccgcgaagc ctgcctggtg
    13681 cgcttccgtc ccatcatgat gaccaccatg gccgcgctgc tcggcacgct gcccatcgcg
    13741 ctcagcatcg gggcgagcgg cgcgtcgcgg cagtcgctcg ggctcgcggt ggtcggcggc
    13801 ctgctcctgt cgcagttcct gacgctctac ctcacgccgg tcgtgtactt gtacctaaac
    13861 cgtttcgaac ggcggagtcc cccgccgggt tagactgcgg acgtgcccag ccgacagaaa
    13921 ctcaccgtct ccgacatcga ggcgctcgtg caggcgcgcc accccgagcc gcgctccctg
    13981 ctcggctacc acgagtacgc ccgcaagggc ggggagccgg cctgcatggt gcgggcgctg
    14041 gagcccgcgg cggagcacgt cgaggtgatc tgggacgacg cgagcgcgcc gagcacgctc
    14101 aagcgcatcc acgacgccgg gcttttcgag gggcgggtcc cctaccgcag gccgttgtcg
    14161 ccgtatcggc tgcgcatccg gtaccgcgac ggcgtcgagc acgtgcggca cgatgcctac
    14221 tacttctcgc cgcagatcac cgacttcgac ctgtacctgt tcggcgaggg caaccaccac
    14281 cgcatctatc tcaagctcgg tgcgcatccg atcgagctcg acggcacgcc gggcacgcgc
    14341 ttcgccgtgt gggcgcccaa cgcggagcga gtgagcgtgg tcggatcgtt caacctgtgg
    14401 gacgggcgcc ggcacgcgat gcagatgcgc ggcagctcgg gcatctggga actgttcgtg
    14461 cccgatgtcg ggcccggcac cacctacaag tacgagatcc gcacgcccga cggccgcacg
    14521 atcctgaaat cggacccctt cgggttcgcg atgcagctgc ggcccgacaa ctgctcggtg
    14581 gtcgcctcgc tggagggaca cgactggggc gaccaggcgt ggatggaggc tcgcggccgg
    14641 gcgcgacacc cggcgctgcc gatcaacatc tacgagttgc atccgggctc ctggcgccag
    14701 cgccgcgatc gtcagccacc gtttcccagc tggcgcgaac tcgccgacga gctgatcccg
    14761 tacgtgcgcg acctgggcta cacgcacgtc gagctgatgg gcgtcgccga gcatccctac
    14821 gaccgctcgt gggggtacca ggtctcgggc tactacgcgc cgacggcgcg ctacggctcg
    14881 ccgcaggact tcatgcactt cgtcgatcgc tgccaccagg ccggcctcgg cgtgatcctc
    14941 gactgggtgc cggcgcattt cccgcgcgac gagcacggcc tcgccgggtt cgacggcacc
    15001 gcgctctacg agcacgcgga cccgcgcctc ggcgagcacg ccgactgggg caccaagatc
    15061 ttcaattacg gccgccacga ggtgcgcaac ttcctcgtcg ccaacgcgct gtactggctc
    15121 gaccgttacc acgtggacgg gctcagggtg gacgcggtcg cgtccatgct ctacctcgac
    15181 tacagccggc gccccggcga at
//
LOCUS       DAOMFD010000034        15163 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_863_length_15163_cov_32.330686, whole genome shotgun sequence.
ACCESSION   DAOMFD010000034 DAOMFD010000000
VERSION     DAOMFD010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15163)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15163)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15163
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_863_length_15163_cov_32.330686"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PL152_03520"
     CDS             <1..593
                     /locus_tag="PL152_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012407.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQR48377.1"
                     /translation="HEIERVTRVAGRLAMQRRRRIVSVDKANVLETSRLWRSVVERVV
                     RDEFPEVALEHMLVDAAAMHLLRKPVSFDVLLTENMFGDILTDEASMLAGSLGLLPSA
                     SLGDGKIGLYEPIHGSAPDIAGRGIANPIGTILSGAMLLRHSLGLEREAAAVEAAIVS
                     AIGAGVRTADLIARGATPVGSRAAAEAVVARLADGI"
     gene            complement(875..2029)
                     /gene="metB"
                     /locus_tag="PL152_03525"
     CDS             complement(875..2029)
                     /gene="metB"
                     /locus_tag="PL152_03525"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HQR48378.1"
                     /translation="MNLKTRTVRAALETDTQHGAVVPPIHLTSTFAFAGFGGKRTYDY
                     SRSGNPTRDALAGALADLEGGAGAVVTSSGMAAVSLVTQLFRPDQVAVVPHDCYGGTY
                     RLFNALRERGSLDVRFVDFQDASALQAALASRPALVWTETPSNPLLRIVDIAAVARAA
                     RAAGALVVADNTFLSPVWQQPISLGADVVIHSTTKYLNGHSDVVGGAVVAAGRELHEQ
                     LAWWANCTGVTGAPFDSYLTLRGLRTLHARMRAHGENTAQVVDMLIRHPAVAKVYYPG
                     LASHPGHAVARSQQSGFGAMVSFELKGDGNAVRALVGELRLFSLAESLGGVESLIAHP
                     ASMTHAAMDAAARERAGISDNLLRLSIGIEDGADLVADLERGLEAAQRSA"
     gene            complement(2045..3073)
                     /locus_tag="PL152_03530"
     CDS             complement(2045..3073)
                     /locus_tag="PL152_03530"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF006449.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HQR48379.1"
                     /translation="MQSGAAAGSTLTVEQLPEGYECLSAVQALPGRFPLHFGGTLESP
                     SVAYRLAGRRGAPVVAVLGGISAGRHVFGLPGEPAGWWEDTIGPGRALDTDRFRVLGI
                     DFLGGSHATTGPRPGESFPSVSAFDQAEVLARLAAHLGIGQFHGCIGASYGGMVALAL
                     AQRHPGRLRQAVVISAAHRTHPMSTAWRSVQRAIVRYALGHGEGSRGLALARGLAMAT
                     YRSAREFEERFSGPAERTADGFRFPVESYLFSRGESYAAQYRPEAFVCLSESIDLHRV
                     DPESITVPVTLVAVVEDQLVPLSDMRELRDRLAGNCRLVEVSSLYGHDAFLKETDVLR
                     DVFAQALD"
     regulatory      complement(3193..3306)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3410..3718
                     /locus_tag="PL152_03535"
     CDS             3410..3718
                     /locus_tag="PL152_03535"
                     /inference="COORDINATES: protein motif:HMM:TIGR02531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YerC/YecD family TrpR-related protein"
                     /protein_id="HQR48380.1"
                     /translation="MKTHKGISHRQEAAAERALFQAVLTLRTVDECRDFFRDLCTPAE
                     LQALADRWSVVGLLQQGIPYREIHRLTGVSVTTVGRVARYLGAGNGGYALAARRLAER
                     "
     gene            3720..4619
                     /gene="hisG"
                     /locus_tag="PL152_03540"
     CDS             3720..4619
                     /gene="hisG"
                     /locus_tag="PL152_03540"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HQR48381.1"
                     /translation="MERNQRLRIAVQKSGRLTEHSLDLLARCGLKYSRGKDQLMCYGE
                     NMPLDVLFVRDDDIPDLVQEDVCDLGIVGLNVIDEKRLAMAARGTQAAFELVQRLDFG
                     RCRLSIAVPDDFPYAGLASIAGKRIATTYPHTLARHLRENSIEAEIVTLSGAVEIAPR
                     LGRADLVCDLVSTGSTLNANHLREVATVLESEAALIRTPVPLSEEKADWVRRLLLRID
                     GVQQVRESKYIMLHAPRSALPEIRKLLPGSESPTVIPLDGQGDKVAVHAVCRENVFWE
                     TLESLKAAGASAVLVLPVEKMLA"
     gene            4616..5941
                     /gene="hisD"
                     /locus_tag="PL152_03545"
     CDS             4616..5941
                     /gene="hisD"
                     /locus_tag="PL152_03545"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HQR48382.1"
                     /translation="MSSPLRILDWSSLTGDERRDSLRRPAQRDTSTLVSSVARIVEEV
                     RARGDRAIREFTGRYDGVDLDAFAVTPAEFDEASAALTKEQIAALRRAIETVTRFHET
                     QSLETVRVQTARGVTCERMFVPIGAVGLYVPAGTAPLPSTAIMLAVPSRLARCPVRIL
                     CTPPRPDGRADPAVLTAARLCGIEQVFKVGGAQAIAAMAYGTESVPKVDKVFGPGNAW
                     VTAAKQFVAADPEGAALDMPAGPSEVLVVADDSAEAEFVAADLLAQAEHSEDAQVVLV
                     TNSRQLADDCVAEVVRQLAALPRKAIAEHAIASSRVLIVPDLDTALEVSNRYAPEHLI
                     LQVRSPRRWLERVRNAGSVFLGAWTPETMGDYCSGTNHVLPTYGHARAYSGLGVLDFV
                     RRMTVQEVTPEGLADLGLTARTLARLESLDGHANAVTVRLDAISRGARS"
     gene            5938..6993
                     /gene="hisC"
                     /locus_tag="PL152_03550"
     CDS             5938..6993
                     /gene="hisC"
                     /locus_tag="PL152_03550"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HQR48383.1"
                     /translation="MTSVLSLARPDILELQPYQHAAWDPSLERMHANEMPWRAQGDNT
                     IAGLNRYPEPQPRALVERMGRLYDVPTARLLVGRGSDEAIDLLVRAFCRAGTDSVVIT
                     PPTFGFYKVAARIQGATVVEVPLRDPDFALDVTAVIEAGRRSKMVFLCSPNNPTGNLL
                     DEQQMLDVADALAGQALVCVDEAYLEFSGRASLASRIDGLPNLVLLRTLSKAYALAGA
                     RCGTLVANEEIVGLLKRIIPPYAIPSSTVEEVIALTEAPQRAISADRIRTILAEREKM
                     RARLVRLASVERVFASDANFLLVRCRDAGRFLDAGRAAGLLVRDFSHSPRLDGCLRIS
                     IGTPEQNERLLSAVERT"
     gene            6990..8060
                     /gene="hisB"
                     /locus_tag="PL152_03555"
     CDS             6990..8060
                     /gene="hisB"
                     /locus_tag="PL152_03555"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003937.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HQR48384.1"
                     /translation="MTAERILFIDRDGTLIEEPADKQVDSLAKVRLAPGVMPALLELK
                     HAGYRFVLVSNQDGLGTDSFPEASFREPQDFLRQLFASQGIEFDAEFFCPHFPADDCH
                     CRKPKTGLLTEYMAGRAIDREASCVIGDRQTDLDLAANLGVQGIRIHGDGGETWPAIA
                     ARLCRPHRVARVQRRTKETDITVAVDLDHESPVVVATGIGFFDHMLEQVARHGGFSLE
                     LTCTGDLQVDEHHTVEDCALALGQALRTALGDKLGIGRYGFVLPMDEALAQVAIDLSG
                     RPYAVFTGRFDREAVGGLPTELVPHFFRSLADSLGAAIHIEVRGENTHHMIEACFKGT
                     GRALRRAFRVRGVDVPSTKGVL"
     gene            8071..8667
                     /gene="hisH"
                     /locus_tag="PL152_03560"
     CDS             8071..8667
                     /gene="hisH"
                     /locus_tag="PL152_03560"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HQR48385.1"
                     /translation="MTEVAIIDNGGANIASLRYALDRLGADSHLTADPEVLSAAPRVI
                     LPGVGAAADAMQRLETSGLVATIRGLRQPVLGICLGMQLLFESSEEGDTRCLGLIAAR
                     VQRFPQRDGYPVPHMGWNQVEFAAAEPLLRGIDTGDYVYFVHGYAAPVGPWTVATTDY
                     GGPFSAVVRQGNFAGTQFHPERSGTTGSRLLANFLELG"
     gene            8667..9407
                     /locus_tag="PL152_03565"
     CDS             8667..9407
                     /locus_tag="PL152_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]
                     imidazole-4-carboxamide isomerase"
                     /protein_id="HQR48386.1"
                     /translation="MRLIPAIDLRGGRCVRLYQGRFDAETVFADDPGTVLESYLAIGA
                     RLLHVVDLDGARAGSQGNARAIASLAADGRARLQVGGGVRDRATVDRLLGAGVARVVV
                     GSAAVTAPDEVAGWLRDFGNEAIVVAFDVRLDDTGIPRLTTHGWEQQTVLSLWDAVGR
                     YLPHGLRHVLCTDVARDGALTGPNLALYQEAVHRFPDVEWQASGGVRDGGDLGALEST
                     GVAAAVSGRALLEGRIPPGELAPFLPSA"
     gene            9422..10156
                     /gene="hisF"
                     /locus_tag="PL152_03570"
     CDS             9422..10156
                     /gene="hisF"
                     /locus_tag="PL152_03570"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HQR48387.1"
                     /translation="MRDGQVVKGVRFRDHRVMGDILDLAARYREEGADELVFYDITAS
                     PEGRSVDRSWVARVARVLDIPFCVAGGIRSIEDAEGVLNAGAEKISVNSPALADPTLV
                     DRLARRFGSQCVVVGIDSQTLDDDYVVYQYTGDPGRSRNTARRTLDWVREVQDRGAGE
                     IVLNCMASDGVRQGYDVEQLAKVRDCCHVPLIASGGAGTLQHFVDVFERARVDGALAA
                     SVFHSGDIRIPDLKRQLAAAHVEVRL"
     gene            10153..10782
                     /gene="hisIE"
                     /locus_tag="PL152_03575"
     CDS             10153..10782
                     /gene="hisIE"
                     /locus_tag="PL152_03575"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF002747.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HQR48388.1"
                     /translation="MTPEQVGTLDWGKDDGLLPAVVQHARTGKVLMLAYMNQESLGLT
                     LSTRRAVFYSRSRQRLWTKGETSGHFLSVVDVSADCDSDAILVIADPLGPTCHKGTES
                     CFADAARTDFEYVAFLPLLEATIAHRDAEKPLGSYTARLLAEGPSRIAQKVGEEGVET
                     ALAAVTRDDPGLVSEAADLLYHLLVLLKSRNLGLKDLVDELRSRHASRP"
     gene            complement(10753..11964)
                     /locus_tag="PL152_03580"
     CDS             complement(10753..11964)
                     /locus_tag="PL152_03580"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR48389.1"
                     /translation="MSLRQQSSAVRNALPRTVVVLGLVSLLNDSASEMITPLLPIFLT
                     ATLGAGPAVVGLVEGVAEATASILKLVSGRLVDRGVRPKALVLGGYTLSNSMRPLIGI
                     AAGWSAVLLLRFLDRVGKGLRTAPRDALIAGAAAPDQRGRAFGFHRSMDHAGAVVGPL
                     LAFALLAANADLKHVFLASIIPGILVLLLLAFGLKPEHAAPIPAPPRFAWSKLHGRLK
                     AMIIAAGMLALASVPEVFVVLWARAAGLAVVWVPLAWAAASVAKMLIAMPAGIASDRV
                     GRVPLLLAGWTLRVIVLLLLAFATAGTIGVWILFMAYSATLAITEPAERSVIGDHAAA
                     EERGTAFGLYHLASGLLVLPGALAFGLIWERFGAAAAFATSAVVTAIAAALMIVLASG
                     DRSGPAGVTRA"
     gene            complement(11968..13413)
                     /locus_tag="PL152_03585"
     CDS             complement(11968..13413)
                     /locus_tag="PL152_03585"
                     /inference="COORDINATES: protein motif:HMM:NF014741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF222 domain-containing protein"
                     /protein_id="HQR48390.1"
                     /translation="MDGANSGANFNAGAGLSTPELEAQITELSGHLNAANRRWLALIA
                     EFDRRKGWHDGLTQTCAHWLNWKCGIDIGAAREKVRVANALEGLPEIGAAMARGELSY
                     SKVRALTRVACPATEQMLLSIALHGTAHHVETVVRQFRRVQEAAEVSREARQLANRRL
                     TYHYDDDGSLVIRAQLPAETGSLVLNALEEAMRGLPLAEHCEDRVHVSAETSRDDIDL
                     YGVSAETFSEDRPTPAARRADALGVLAESFLKHGPEALNGGERHQIVVHVDAESLHAR
                     DSGRCELDDGPAIPIETARRLSCDASVVRILENGEGEPLDVGRKTRTIPPALRRALKS
                     RDQGCVFPGCTHKRYVDGHHIHHWAEGGETKLANLVSLCRTHHRAVHEGGIVVQRLDD
                     GAWRFVQRSGDVLHSCAPGSSRPPADWAQLPAAHRELGIVIDAGTAVTRWRGERMDHV
                     WTIDLLVAKMERARSLARGGRAVRGDADGCR"
     gene            complement(13533..14660)
                     /gene="asd"
                     /locus_tag="PL152_03590"
     CDS             complement(13533..14660)
                     /gene="asd"
                     /locus_tag="PL152_03590"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004641000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HQR48391.1"
                     /translation="MKTGIVGWRGMVGSVLMQRMGEERDFEHIEPVFFSTSQAGGAAP
                     DVGKRVGPLQDANDLAALAACETVLSCQGGDYTNDVHPRLRAKGWKGYWIDAASALRM
                     KDDAIIILDPVNRHVIDAGLRAGIRDYVGGNCTVSLMMLGLAGLFRAGLVEWITTMTY
                     QAASGAGAPNMRELVEQMGAAHASAAGLLKDPASAILDIDRAVTERLRSAELPTAQFG
                     APLAGSLIPWIDKDMGNGVSREEWKGTAETNKILGRVTDPIPVEGICVRIGAMRCHSQ
                     AMTIKLKRDLPLAEIEQLIAGANEWVRLVPNDRESSIRELSPVRVSGTLGIPVGRVRK
                     LAMGGQYIAAFTVGDQLLWGAAEPLRRMLRILLEQGSTHAG"
     gene            complement(14678..>15163)
                     /locus_tag="PL152_03595"
     CDS             complement(14678..>15163)
                     /locus_tag="PL152_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HQR48392.1"
                     /translation="VMDLGGGLGVPEKPGQVPVDLTMLQGVIEDLRTRWPALAIWLEP
                     GRYLVAQAGVLLTQVTQMKGKGAVQYIGVATGMNSLIRPALYGSHHDIVNLSRLGEPG
                     TEVYNVVGPICESADYLGHERLLPPTAEGDTILIANAGAYGHAMSSWYNLREPAREVI
                     L"
BASE COUNT         2395 a         5289 c         5140 g         2339 t
ORIGIN      
        1 cgcacgagat cgagcgcgtg acccgcgtgg ccgggcgcct ggcgatgcag cgacgccggc
       61 ggatcgtctc ggtggacaag gcgaatgtgc tcgagacctc gcggctctgg cgctcggtcg
      121 tcgagcgcgt cgtccgtgac gagtttccgg aggtcgcact ggaacacatg ctggtggacg
      181 ccgctgcgat gcacctgctg cgcaagccgg tctccttcga cgtcctgctg acggaaaaca
      241 tgttcggaga catcctgacc gacgaggcat ccatgctggc cgggtcgctc ggattgctgc
      301 cgtccgcctc gctgggcgac ggcaagatcg gactgtacga accgatccac ggctctgccc
      361 cggacatcgc aggtcgcggc atcgcgaacc ccatcggaac gatcctcagc ggcgcgatgt
      421 tgctcaggca ttcgttgggg ctcgagcgtg aggcggctgc cgtcgaagcc gccatcgtgt
      481 cggccatcgg cgccggggtc cggaccgcag acctgatcgc ccggggtgcc acgccagtcg
      541 gcagccgggc ggctgcggaa gcggtcgtgg cgcggctggc agacggcatc tgattgttta
      601 taaatcagat agttacggca ttttcaagac atgctgcata aaggccgtga tggcggcccc
      661 gcccggtccg aacccaccgt gatttcggtg tacatgcgcc ggaaatccag cgctatactg
      721 cgcgtccgcg aacgagtccg gccggctttg atggtccccc gactcgacag cgcgacggtt
      781 gagcaggact tgagtcgggt tagtcattgc ctcacggatt gaggcaactc gggtcctact
      841 tcgagaggcc ggcttcaccg ccggcctttc ttttttacgc cgaccgctgc gccgcctcga
      901 gcccccgctc gaggtccgcc acgaggtctg ccccatcctc gatgccgatc gacaaccgca
      961 acaggttgtc ggaaatgccc gcacgctcac gggctgcggc atccatcgcc gcgtgagtca
     1021 tgctcgccgg atgcgcgatg aggctttcga cgccgcccag tgactccgcg agggagaaca
     1081 ggcgcaactc gcctaccagc gcacgcacgg cgttgccgtc gcccttgagt tcgaagctca
     1141 ccatcgcgcc gaaaccactc tgctggctgc gtgccaccgc gtgtccgggg tgcgacgcga
     1201 ggcccgggta atacaccttg gcgacggccg ggtggcggat cagcatgtcg acgacctgcg
     1261 cggtgttctc accgtgcgcg cgcatgcgcg cgtgcagcgt gcgcaaaccg cgcagcgtga
     1321 ggtagctgtc gaacggcgct cccgtcacgc cggtgcagtt cgcccaccac gccagttgct
     1381 cgtgcaactc acgcccggcc gcgaccaccg cacccccgac cacgtcgctg tgaccgttca
     1441 ggtacttggt cgtggagtga atcaccacgt ccgcacctag tgagattggc tgctgccata
     1501 cgggcgacag gaacgtgttg tccgccacca cgagtgcgcc ggccgcacgg gccgcacggg
     1561 cgacggcggc gatgtcgacg atgcgcagca gcgggttgct gggcgtctcg gtccagacca
     1621 gcgcgggtcg ggatgccagc gccgcctgca gcgccgatgc atcctggaag tccacgaacc
     1681 gcacgtcgag cgagcctcgc tcccgcaagg cattgaagag ccggtaggtg ccaccgtagc
     1741 agtcgtgcgg caccaccgcg acctggtccg gccggaacag ctgcgtcacg agcgagacgg
     1801 ccgccatgcc cgatgacgtc accacggcac ccgcgcctcc ctcgagatcc gccagcgccc
     1861 cggccagcgc gtcacgcgtc ggattgccgg atcgcgagta atcgtaggtg cgcttgccgc
     1921 cgaagcccgc gaacgcgaac gtcgaggtca ggtggatcgg cggcacgacg gcgccgtgct
     1981 gcgtgtcggt ttcgagcgcg gcacgcacgg tgcgcgtctt caggttcatg tcgttgctca
     2041 tgggtcagtc gagggcctgc gcgaacacgt cgcgcagcac gtcggtctcc ttcaggaagg
     2101 cgtcatgtcc atagagcgag ctcacctcga ccagccggca attgccggcc aggcggtcgc
     2161 gcagctcgcg catgtcggaa aggggcacca gctggtcttc caccacggcg accagcgtca
     2221 cgggaacggt gatcgattcg ggatcgaccc ggtgcaggtc gatcgactcg gacaggcaga
     2281 cgaatgcctc gggccggtac tgggccgcgt aggattcgcc gcgggaaaac aggtaggact
     2341 cgaccgggaa ccggaacccg tccgccgtgc gttcggcggg ccccgagaag cgttcctcga
     2401 attcccgcgc ggaacggtag gttgccatgg cgagcccgcg tgccaacgcc aggccgcggc
     2461 tcccctcgcc gtgccccagc gcgtaacgca cgatggcgcg ctggacgctg cgccaggccg
     2521 tggacatcgg gtgggtgcgg tgcgccgcgc tgatcacgac cgcctgcctg agccgacccg
     2581 ggtgccgctg cgcgagcgcg agcgccacca tgccgccgta cgacgcgccg atgcagccgt
     2641 ggaactgtcc gatccccagg tgagctgcga gccttgcgag cacttcggcc tggtcgaagg
     2701 cgctgacgct cgggaacgac tcgcccggcc gaggcccggt cgtcgcgtgg ctgccgccca
     2761 ggaaatcaat gccgagcact cgaaaacgat cggtgtcgag ggcccgtccg ggcccgatcg
     2821 tgtcctccca ccagcctgcc ggctcgccag gcagtccgaa tacgtgccgc ccggccgaaa
     2881 tgccgcccag gaccgccacg acgggcgcgc cccgccgccc cgccagccgg tatgcgacgc
     2941 tgggcgactc gagcgtgccg ccgaaatgca gcgggaaccg gcccggcagc gcttgcacgg
     3001 cggacaggca ctcatatccc tcgggcagct gctcgacggt gagagtggat ccagcggcgg
     3061 ctcctgactg catctcgcgt acgtgctcct cggaccggcc atccggaccg gctgcaggct
     3121 tcgagatcga cgggagtacg atggttgccg cgacccacac ccccggggcg aactccgggg
     3181 aagctacctg gcaactcatc tgtcggcgga ccgagtcccc gtggagttgg caccttttcg
     3241 ggcgggttgg acccgacggt tgccccggct tcaaagggcc attccctcag ccggtctcga
     3301 tgagcggcgg cagtttaggt gccggcagcg cgcagcgtca aggcgcgccg cttgcgccgg
     3361 atcacgccat gtattaatgt gctagcacgc tattacattg ggccgggtcg tgaagacaca
     3421 caagggcatt tcccaccgcc aggaggccgc cgcggaacgc gcgctgttcc aggcggtgct
     3481 gaccctgcgg accgtcgacg agtgccgcga cttcttccgc gacctgtgca cgccggccga
     3541 gctgcaggcc cttgccgatc gctggagtgt cgtgggcctc ctgcagcagg gcatccccta
     3601 ccgggagatc caccggctca ccggggtcag cgtcaccacc gtcgggcgcg tggcccgcta
     3661 tctcggcgca ggcaacggcg gctacgcgct cgccgcccgg cgcctcgcgg agcgttgaga
     3721 tggagcgcaa tcagagactg cggatcgccg tccagaagtc cggccgcctg accgagcact
     3781 cgctcgacct gctggcccgc tgcggcctca agtacagccg cggcaaggac cagctgatgt
     3841 gctacggcga gaacatgccg ctcgacgtgc tgttcgtgcg cgacgacgac atccccgacc
     3901 tcgtgcagga ggacgtctgc gacctcggca tcgtcgggct gaacgtgatc gacgagaagc
     3961 ggctggccat ggcggcccgc ggcacgcagg ccgccttcga gctcgtccag cgcctcgatt
     4021 tcgggcgctg tcgcctgtcg attgcggtcc cggacgattt tccatacgcc ggactggcga
     4081 gcatcgccgg caagcgcatc gccaccacct acccccatac gctcgcgcgc cacctgcgcg
     4141 agaactcgat cgaggcggag atcgtgacgc tctccggcgc cgtcgagatt gcgccgcggc
     4201 tgggacgggc cgacctcgtc tgcgacctcg tctcgaccgg ttccacgctg aacgccaatc
     4261 acctgcgcga ggtcgcgacg gtcctggaga gcgaggcggc gctgatccgc acgccggtcc
     4321 cgctgtcgga ggagaaggcc gactgggtgc gccggctgct gctgcgcatc gacggcgtgc
     4381 agcaggtccg ggagagcaag tacatcatgc tgcacgcgcc ccgctccgcc ctgcccgaga
     4441 tccgcaagct gctgccgggc agcgaatcgc ccaccgtcat cccgctcgac ggccagggcg
     4501 acaaggtcgc cgtgcacgcg gtgtgccgcg agaacgtgtt ctgggagacg ctcgagagcc
     4561 tcaaggcggc aggcgccagt gcggtgctgg tgctgccggt ggagaagatg ctggcatgag
     4621 cagcccactg cgcattctcg actggtcgtc gcttacgggc gacgagcgac gggactcgct
     4681 gcgccggccc gcgcagcggg acacctccac gctcgtttca agcgtcgcgc gcatcgtcga
     4741 ggaagtccgc gcgcggggcg atcgcgcgat ccgcgagttc acgggtcgct acgacggcgt
     4801 ggacctcgac gccttcgccg tgacgcccgc ggaattcgac gaggcctccg ccgcgctcac
     4861 caaggagcag atcgcggcgc tgcggcgcgc gatcgagacc gtgacgcgat tccacgagac
     4921 ccagtcgctg gaaacggtgc gcgtgcagac ggcacgcggc gtgacctgcg agcgcatgtt
     4981 cgtcccgatc ggcgctgtcg gcctgtacgt gcctgcgggc acggctcccc tgccttccac
     5041 cgccatcatg ctggccgtgc cgtcccgtct cgctcgctgc ccggtccgca tcctgtgcac
     5101 gccgccgcgg cccgatggtc gcgccgaccc cgcggtgctc acggccgcgc gactgtgcgg
     5161 catcgagcag gtgttcaagg tcggcggcgc ccaggccatc gcggccatgg cgtacggcac
     5221 ggagagcgtg cccaaggtcg acaaggtctt cgggccgggc aacgcctggg tgaccgcggc
     5281 gaaacagttc gtcgcggccg atccggaagg cgcggcactc gacatgccgg ccgggccttc
     5341 cgaagtgctc gtcgtcgctg acgactccgc cgaagccgag ttcgtcgcgg ccgacctcct
     5401 cgcgcaggcc gagcacagcg aggacgcaca ggtcgtcctg gtgacgaatt cgcgccagct
     5461 ggccgatgat tgcgtggcgg aggtcgtgcg ccagctcgcc gcgttgccgc gcaaggcgat
     5521 cgccgaacac gccatcgcga gcagccgggt gctgatcgtg cccgacctcg acaccgcgct
     5581 cgaggtgagc aaccgctatg ccccggagca cctgatcctg caggtccgat cgcctcgccg
     5641 ctggctcgag cgcgtgcgca atgccggctc ggtgttcctc ggcgcgtgga ctcccgagac
     5701 gatgggcgac tactgcagtg gcacgaacca cgtgctgccc acctacggtc atgcgcgggc
     5761 gtacagcggg ctcggcgtgc tggatttcgt gcggcgcatg acggtccagg aggtcacgcc
     5821 cgaagggctc gcggacctcg ggctcacggc ccgcaccctg gcccggctcg agtcgctgga
     5881 cggccacgcg aatgccgtga cggtccggct cgacgcgatc tcccgcggag cgcggtcatg
     5941 acatcggtgc tgtccctggc gaggcccgac atcctggagt tgcagccgta ccagcacgcg
     6001 gcgtgggatc cgtcgctcga gcgcatgcac gcgaacgaga tgccgtggcg ggcgcagggc
     6061 gacaacacga tcgccgggct caaccgctat cccgagccgc agccccgcgc gctcgtcgag
     6121 cgcatgggcc gtctgtacga cgtcccgacg gcccggctgc tcgtcggccg tggcagcgac
     6181 gaggcgatcg acctgctggt gcgggcgttc tgccgcgccg gcaccgacag cgtcgtcatc
     6241 acgccgccga ccttcgggtt ctacaaggtc gccgcgcgca tccagggcgc caccgtcgtc
     6301 gaggtcccgc tgcgcgaccc ggacttcgcg ctcgacgtga cggcggtcat cgaggccggc
     6361 cgtcgctcga agatggtctt cctgtgctcc ccgaacaacc cgaccggcaa tctcctcgat
     6421 gagcagcaga tgctcgacgt cgccgatgcc ctcgccgggc aggcgctggt ctgcgtggac
     6481 gaggcgtacc tcgaattctc gggccgcgcg agcctggcat cccggatcga cgggctgccg
     6541 aacctcgtgc tgctgaggac gttgtcgaaa gcctatgcgc tcgcaggcgc gcgctgcggc
     6601 acgctggtcg cgaacgagga aatcgtcggc ctgctcaagc gcatcatccc gccctatgcg
     6661 atcccgtcgt ccacggtcga ggaagtgatc gcgctcaccg aagcgccgca gcgagcgatc
     6721 tcggctgacc gcatccgtac gatcctggcc gagcgcgaga agatgcgcgc gcgactcgtg
     6781 cgactcgcct ccgtcgagcg ggtttttgcg tccgacgcga acttcctgct cgtccgctgc
     6841 cgggacgcag gccgattcct cgacgccggc agggccgccg gcctgctggt acgcgatttc
     6901 agccactccc cgcgactgga cggctgcctg cggatctcga tcgggacgcc ggagcagaac
     6961 gagcgactgc tctcggccgt ggagcgcaca tgaccgccga acgcatcctg ttcatcgacc
     7021 gcgacggcac gctcatcgag gagcccgcgg acaagcaggt cgacagtctc gcgaaggtcc
     7081 ggctggcgcc gggcgtcatg ccggcactgc tcgagctgaa gcacgcgggt tatcgcttcg
     7141 tgcttgtgag caaccaggac gggctcggaa cggattcgtt ccccgaggcg agcttccgcg
     7201 agccgcagga cttcctgcgg cagctgttcg cgtcgcaggg catcgagttc gacgccgagt
     7261 tcttctgccc gcattttccc gcggacgact gccactgccg caagccgaag acgggcctgc
     7321 tcacggagta catggctggc cgcgccatcg accgcgaggc gagctgcgtg atcggcgacc
     7381 ggcagaccga cctggacctg gcggccaatc tcggcgtcca ggggatacgc atccacggcg
     7441 acggcggcga gacctggccc gccatcgccg cacgcctctg ccgcccgcat cgcgtcgcgc
     7501 gggtgcagcg gcgaacgaag gaaacggaca tcacggtcgc cgtcgatctc gatcatgaat
     7561 caccggtcgt cgtggccacg ggcatcgggt tcttcgacca catgctcgag caggtcgccc
     7621 ggcacggcgg gttctcgctc gaactcacgt gcaccggcga cctgcaggtg gacgagcacc
     7681 acacggtcga ggactgtgca ctggcgctcg gccaggcgct gcgcacggcg ctgggcgaca
     7741 agctcggcat cgggcgctac ggcttcgtgc tgcccatgga cgaggcactc gcccaggtcg
     7801 cgatcgacct gtcgggccgc ccctacgcgg tattcaccgg gcgcttcgac cgcgaggccg
     7861 tcggtggcct gccgaccgag ctcgtgcccc acttcttccg gtcgctcgcg gacagcctcg
     7921 gcgcggccat acacatcgag gtccgcggcg agaacacgca ccacatgatc gaggcctgct
     7981 tcaagggaac cgggcgtgcc ctgcgtcgag cgttccgcgt gcgcggcgtg gacgtaccca
     8041 gcaccaaggg cgtgctctga ggcacctccc gtgaccgagg tggccatcat cgacaacggc
     8101 ggcgccaaca tcgcgtcgct gcgttacgcg ctcgaccgcc tcggcgccga ctcgcacctc
     8161 accgcggatc ccgaggtgct gagcgcggct cccagggtga tcctgccggg cgtcggcgcg
     8221 gcggcggacg ccatgcagcg cctcgagaca tcgggcctcg tcgcgaccat tcgcgggctc
     8281 cggcagcccg tgctcggcat ctgcctgggg atgcagctgc tcttcgagtc cagcgaggaa
     8341 ggcgacaccc ggtgcctcgg gctgatcgcg gcacgtgtgc aacgcttccc gcagcgcgat
     8401 ggctaccccg tgccgcacat gggctggaac caggtcgagt tcgccgctgc ggagccgctg
     8461 ctgcgtggta tcgacaccgg tgactacgtg tacttcgtgc acggctacgc cgcaccggtc
     8521 ggtccctgga ccgtcgcgac caccgactac ggcggtccct tctccgccgt cgtgcgacag
     8581 ggaaatttcg cgggcacgca gttccacccg gagcgctccg gcaccaccgg ttcgcggctc
     8641 ctggccaatt tcctggagct gggttgatga gactgatccc ggccatcgac ctgcgaggcg
     8701 gccgctgcgt gcgcctgtac cagggccgct tcgacgcgga aaccgtcttc gccgacgacc
     8761 cgggcacggt gctcgagtcc tacctcgcga tcggagcgcg ccttctgcac gtcgtcgacc
     8821 tcgacggagc gcgcgccggt tcccagggca atgcgcgggc catcgccagc ctggcggccg
     8881 acgggcgtgc ccggctccag gtcggcggcg gcgtgcgcga tcgggcgacg gtggaccggc
     8941 tgctcggtgc cggcgtcgcg cgggtcgtgg tcgggagtgc cgccgtgacg gcgcccgacg
     9001 aggtggcggg atggctgcgc gatttcggaa acgaagcgat cgtcgtcgcc ttcgacgtcc
     9061 ggctcgacga cacggggatt ccccgcctca ccacgcacgg ctgggaacaa cagaccgtgc
     9121 tcagcctatg ggatgcggtg gggcgctact tgcctcacgg cttgcggcac gtgctctgca
     9181 cggacgtggc gcgcgacggg gccctgaccg ggccgaacct cgccctgtac caggaagcgg
     9241 tgcaccgatt cccggacgtg gaatggcagg cctcgggcgg cgtgcgcgac ggcggcgacc
     9301 tgggtgcact cgaatccacg ggcgtcgcgg cggccgtgag tggccgcgca ctgctggaag
     9361 gccgcatacc accaggggag ctcgcgccat tcttgccaag cgcataatcc cgtgcctcga
     9421 cgtgcgcgac ggccaggtcg tcaagggcgt gcgcttccgc gaccatcgcg tcatgggtga
     9481 catcctcgat ctcgcggccc gctaccgtga agaaggcgcg gacgagctgg tcttctacga
     9541 catcaccgcc agcccggagg gccgctcggt ggaccgcagc tgggtggcgc gggtcgcgcg
     9601 cgtgctcgac atcccgttct gcgtcgcggg aggcatccgc agcatcgagg atgccgaagg
     9661 cgtgctgaac gccggtgccg agaagatctc ggtcaactcc ccggcgctcg ccgatccgac
     9721 gctcgtcgat cgcctggcgc ggcggttcgg ttcgcaatgc gtggtcgtcg ggatcgacag
     9781 ccagacgttg gacgacgatt acgtcgtgta tcagtacacg ggtgatcccg ggcgctcgcg
     9841 caacactgcg cgccgcaccc tcgactgggt gcgcgaagtg caggaccgcg gcgccgggga
     9901 aatcgtgctc aattgcatgg ccagcgacgg cgtgcggcag ggctacgacg tcgagcagct
     9961 cgcgaaggtg cgggactgct gccacgttcc gctgatcgcg tcgggtggtg ccggcacact
    10021 gcagcatttc gtcgacgtgt tcgagcgggc gcgcgtcgat ggcgcactgg ccgccagcgt
    10081 gtttcactcg ggcgacatcc gcattcccga cctcaagcgg caactggccg cggcgcacgt
    10141 ggaggtgaga ctgtgacacc ggaacaagtc ggcacgctcg actggggcaa ggacgacggg
    10201 ctgctgcccg ccgtcgtgca gcacgcgcgc acgggcaagg tgctgatgct ggcgtacatg
    10261 aaccaggagt ccctgggcct cacgctgtcc acgcgtcgtg cagtgttcta cagccgctcg
    10321 cgccagcggc tctggaccaa gggcgagacc agcggccact tcctgagcgt ggtcgacgtg
    10381 agcgcggact gcgactccga cgcgatcctg gtcatcgccg accccctggg accgacctgt
    10441 cacaagggca ccgagtcctg cttcgcggac gccgcgcgca cggacttcga atacgtcgca
    10501 ttcctgccgc tgctcgaggc gacgatcgcg catcgcgacg ccgagaagcc gctgggcagc
    10561 tacacggccc gcctgctggc ggagggtccc tcgcgaatcg cacagaaggt gggcgaggaa
    10621 ggcgtcgaaa ccgcgcttgc cgccgtcacc cgggacgatc ccgggctggt gagcgaggcg
    10681 gccgacctcc tctaccacct gctggtgctg ctgaagtccc ggaacctcgg gctcaaggac
    10741 ctcgtcgacg aactacgctc gcgtcacgcc agccggccct gaccggtcgc ccgacgcgag
    10801 cacgatcatc aacgcggcgg cgatggcggt cacgactgcg gaggtcgcga atgccgcggc
    10861 cgcgccgaat cgctcccaga tgaggccgaa cgcgagcgcg ccgggcagga ccagcagacc
    10921 gctcgcgagg tgatacaggc cgaaggccgt gccgcgttct tcagccgccg catggtcccc
    10981 gatcacggaa cgctcggctg gctcggtgat ggcgagtgtc gccgaatagg ccatgaacag
    11041 gatccagacg ccgatcgtgc cggcggtggc aaatgccagc aacagcagca cgatcacacg
    11101 cagggtccag ccagccagca gcagcggcac acggccgacg cggtcggacg cgattccggc
    11161 cggcatcgcg atgagcatct tcgcgacgct ggccgctgcc cacgcgagcg gcacccagac
    11221 caccgccagg ccggcggccc tcgcccacag gacgacgaac acctcgggca cgcttgcgag
    11281 cgccagcatt ccggccgcga tgatcatggc cttcaggcgg ccgtgcagct tgctccaggc
    11341 gaagcgcggc ggcgcgggaa tcggcgcggc gtgctctggc ttcagtccga aggccaacag
    11401 cagcagcacc aggatgccgg ggatgatcga ggccaggaac acgtgcttca agtcggcgtt
    11461 ggccgcgagc agcgcaaatg cgagcaatgg acccacgacc gccccggcgt ggtccatcga
    11521 gcgatggaat ccgaaagcgc ggcctcgttg atcgggcgcc gcggcgccgg caatgagcgc
    11581 atctcgcggg gctgtccgga ggcccttgcc gacgcggtcc aggaaacgca gcagcagcac
    11641 cgcagaccag ccggccgcga tgccgatcaa cggccgcatc gaattggaca gcgtgtatcc
    11701 gccgagcacc agcgccttgg gccgcacgcc gcggtcgacg agccggcccg acaccagctt
    11761 gaggatgctc gccgtggcct cggcgactcc ctccaccagt ccgacgaccg cagggcccgc
    11821 ccccagcgtg gccgtgagga agatcggcag cagcggcgtg atcatctcgc tggccgaatc
    11881 gttcagcagc gagacgaggc ccaggacgac gaccgtgcgc ggcagtgcat tgcgaacagc
    11941 gctggattgc tgccgcaggc tcatgagtca ccggcaacca tcggcgtcgc cccgaactgc
    12001 ccgtccaccg cgcgccagcg accgagcccg ctccatcttg gccacgagaa gatcgatcgt
    12061 ccacacgtgg tccatgcgct caccgcgcca tctcgtgact gcagttcctg cgtcgatgac
    12121 gattcctagc tcacgatggg ccgccggaag ttgcgcccag tccgccggcg gccggctgga
    12181 acccggcgcg caggagtgca gtacatcgcc gctgcgctgc acgaagcgcc aggcgccgtc
    12241 atcgagacgc tgaacgacga tgccgccctc gtgcaccgcg cgatggtgcg tgcggcacaa
    12301 cgagaccaga ttggcgagct tcgtctctcc gccctcggcc cagtgatgaa tgtgatggcc
    12361 atccacgtaa cgtttgtgcg tgcacccggg gaagacgcag ccctgatcgc gggacttgag
    12421 cgcgcggcgc agggccggag ggatggttcg cgtcttgcgg cccacgtcca gcggttcgcc
    12481 ctcaccattt tcgaggatcc tcacgacact tgcgtcgcac gacaggcggc gggcggtctc
    12541 gattggaatg gcaggaccat cgtcgagctc gcatcgccct gaatcgcgcg cgtgcagcga
    12601 ctcggcatcg acgtgcacga cgatctggtg ccgctcgccg ccgttcaggg cctccgggcc
    12661 gtgcttcagg aaactctcgg ccagcacgcc aagcgcgtcg gcacgcctgg ccgccggtgt
    12721 tggtcgatcc tccgaaaacg tttcagcgga aacgccgtac aagtcgatat cgtcccgtga
    12781 cgtttcagcg gaaacgtgaa cgcgatcctc acaatgctcg gccaatggga gaccgcgcat
    12841 cgcctcttcg agcgcattca gcaccagcga gccggtctca gcggggagct gcgcccggat
    12901 cacgagtgat ccatcgtcgt cgtaatggta ggtgagccga cgattcgcga gctgcctcgc
    12961 ctcgcgcgac acctcggccg cctcctgcac ccgacgaaac tgtcgcacga ccgtctcgac
    13021 gtgatgcgcc gtaccgtgga gcgcgatgct gagcagcatc tgctcggtgg ccgggcatgc
    13081 tacgcgggtc agggcacgaa ccttggaata gctcagttcg ccacgggcca tcgcagcgcc
    13141 gatctccgga agaccttcga gcgcgtttgc cacgcggact ttctcgcgag cggcaccgat
    13201 gtcgatgccg cacttccagt tgagccagtg ggcgcaagtc tgggtgagtc cgtcgtgcca
    13261 gcccttgcgg cgatcgaact cggcgatcag ggccagccag cgccggttgg cggcgttcag
    13321 gtggccggaa agttcggtga tctgcgcctc gagctccggc gttgaaaggc cagcccctgc
    13381 attgaaattt gcacccgaat tcgcaccgtc catgacgctc tccgaggggc tgttgttata
    13441 tacagtatga tacttgaatt gcctttattt gcaataggtt aggcgtcctt tgaagcttcg
    13501 aaaacagcga aaaattgcga tccaacaaac gatcaaccgg cgtgtgtcga accctgctcg
    13561 agcaggatgc gcagcatgcg gcgcagcggc tcggccgcgc cccagagcaa ctggtcgccg
    13621 accgtgaatg cggcaatgta ctgcccgccc atcgcgagct tgcgcacgcg tccgacgggt
    13681 atgccgagcg tgccgctgac ccgcaccggg ctcagttccc gaatcgacga ctcgcggtca
    13741 ttcggaacca accgcaccca ttcgttggcg cccgcgatca actgctcgat ctcggccagc
    13801 gggaggtcgc gcttgagctt gatggtcatc gcctggctgt ggcagcgcat ggcgccgatg
    13861 cgcacgcaga tcccctcgac cggaatcggg tccgtcacgc gacccagtat cttgttggtc
    13921 tccgccgtgc ccttccattc ctcgcggctg acgccgttgc ccatgtcctt gtcgatccag
    13981 ggaatgaggc tgccggcgag cggcgccccg aactgcgccg tcggcagttc ggcactgcgc
    14041 aggcgctcgg tcaccgcgcg atcgatgtcg aggatggcgc tcgcggggtc cttcagcaga
    14101 cccgcggcgg acgcatgcgc tgcgcccatt tgctcgacga gttcgcgcat gttgggagcg
    14161 cccgcgccgg acgccgcctg gtaggtcatc gtcgtgatcc actcgacgag cccggcgcgg
    14221 aacaggccgg cgagcccgag catcatgagc gaaaccgtgc aattgccgcc gacgtagtcg
    14281 cggatcccgg cgcgcagacc cgcatcgatc acgtggcgat tgacggggtc gaggatgatg
    14341 atcgcgtcgt ccttcatgcg cagcgcggag gcggcgtcga tccagtagcc cttccatccc
    14401 ttggcacgca ggcgcggatg cacgtcgttg gtgtagtcgc cgccctggca gctcaagacg
    14461 gtctcacagg ccgccagcgc ggccaggtcg ttcgcgtcct gcagcggtcc gacgcgcttg
    14521 cccacgtccg gcgcggcgcc gcccgcctgg gacgtgctga agaacacggg ctcgatgtgc
    14581 tcgaagtcgc gctcctcgcc catgcgctgc atgagcaccg agcccaccat tccacgccag
    14641 ccgacgatgc ctgtcttcac gcgttactcc gccgggatca gaggatcact tcgcgcgccg
    14701 gctcgcgcag gttgtaccag gaactcatgg cgtggccgta ggcgcccgcg ttcgcgatga
    14761 ggatcgtatc cccctccgcg gtcggaggga gcagtcgctc gtggccgagg tagtccgccg
    14821 actcgcagat cggaccgacg acgttgtaaa cctcggtgcc cggctcgccg agccgcgaca
    14881 ggttgacgat gtcgtggtgc gagccgtaca gcgccggacg gatgagcgag ttcatccccg
    14941 tcgcgacgcc gatgtactgc acggcgccct tccccttcat ctgcgtgacc tgggtcagca
    15001 gcacgccggc ctgtgcgacg aggtaacggc cgggctcgag ccagatcgcg agcgcgggcc
    15061 agcgcgtccg caggtcctcg atcacgccct gcagcatcgt gaggtcgacc ggcacctgcc
    15121 cgggcttctc gggcacgccg aggccgccgc cgaggtccat gac
//
LOCUS       DAOMFD010000035        15159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_865_length_15159_cov_38.153999, whole genome shotgun sequence.
ACCESSION   DAOMFD010000035 DAOMFD010000000
VERSION     DAOMFD010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 15159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15159
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_865_length_15159_cov_38.153999"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="PL152_03600"
     CDS             complement(<1..308)
                     /locus_tag="PL152_03600"
                     /inference="COORDINATES: protein motif:HMM:NF013500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HQR48393.1"
                     /translation="MNERAAAFGAGPVHVAPLTASATAAVCALARSLGLDTTRIDLFD
                     CRSKSELLDRVAAALDFPSTFGHNWDALFDCLADLGWRPATGYVLVFEHAAALRKTEP
                     "
     gene            complement(305..709)
                     /locus_tag="PL152_03605"
     CDS             complement(305..709)
                     /locus_tag="PL152_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease domain-containing protein"
                     /protein_id="HQR48394.1"
                     /translation="MSGKLRIVVVALALIAGMQWWSGHHGTGPDSAGAAASSVASAHY
                     PDFLPPEAVDTLEAIRRGGPFPYARDGVVFQNRERLLPAQPAGYYREYTVPTPGSADR
                     GARRIVTGGDPPEAYYYTDDHYRSFRRVEIHP"
     gene            complement(755..1468)
                     /locus_tag="PL152_03610"
     CDS             complement(755..1468)
                     /locus_tag="PL152_03610"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HQR48395.1"
                     /translation="MRMTSVFGRLGGGRRVRVGLVAVATFMVASPAWSADVPHSGFLP
                     DYSQLKPAKSAAGNKLERWLSPKLTRENYHAMIVDDVVFYPTPEATEQVPQQTLDDIL
                     TYLTHSVREVALREVPQVTEPGPGVARLRVAVTAVAVGSTGMKPYEFVPAALVFSTAM
                     RAAGQRSQDVMLSVEAIITDSVTGEPLGMVVRHGPGQQLKNAKTPLTLDQLKARIDNW
                     AEAAAATVDQRLKQKSAAD"
     gene            2142..2876
                     /locus_tag="PL152_03615"
     CDS             2142..2876
                     /locus_tag="PL152_03615"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQR48396.1"
                     /translation="MPAEAARAPVNCDEANPFITGSAAVEFARACAATNPFLSGTGIA
                     TVAMNAPSGSFGSGAGPALATPGPTAEGQGAGSAPAVSGGTMPFGGSYEDAIASGPRS
                     LETADRVLVKKSERMLYLMRADRVIAEYPIKLGLNPYGPKRKQGDFRTPEGDYRLVRR
                     NSHSDFFLALEVSYPNEADVERAREMGADPGGLIMIHGQPNTPRKPASYYASKDWTDG
                     CIAVTNAAMVDIWLRTDVGTPIEIRP"
     gene            2876..4276
                     /gene="ppnN"
                     /locus_tag="PL152_03620"
     CDS             2876..4276
                     /gene="ppnN"
                     /locus_tag="PL152_03620"
                     /EC_number="3.2.2.10"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase PpnN"
                     /protein_id="HQR48397.1"
                     /translation="MHPAEVVVAAGDDQDSVDARVTPTGSLENLSQQEVDRLLDSSRG
                     GLYPLFRRCSLAVLNAGGETDDARAIFERYADYEIRIVRQAWGIKLELRNAPAGAFVD
                     GRMIRGIQEHLFAVLRDILYIHGEVYEHGSVELTDTQGITNAVYRILRNAGVLRPRVR
                     PDIVVCWGGHSIGREEYDYTKRVGYELGLRGLNVCTGCGPGAMKGPMKGATIGHAKQR
                     IHDGRYIGITEPGIVAAEPPNAIVNQLVIMPDIEKRLEAFVRTGHGILVFPGGAGTAE
                     EILYLMGILLDPANAQQPFPVVFTAPESSREYFAQIDAFLAATLGQRVRDRYRIVVGD
                     PAEAAREMQRGMDAVREFRRRASDAYNYSWTLAIDQELQRPFEPTHEAMAQLDLDREL
                     PPHRLAAQLRRAFSGIVCGNVKDHGIRLIEQHGPFEIHGDKELLAPLDALLESFVRQR
                     RMRLAADYRPCYRVVT"
     gene            4354..4605
                     /locus_tag="PL152_03625"
     CDS             4354..4605
                     /locus_tag="PL152_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48398.1"
                     /translation="MTDHKNPSSDPAPAETAEPSAPGRRSKFGAAADGWDAYNDWLER
                     VRAQQGRISRQAVVAKSLYTVSSYRNWADKARDAFDKGK"
     gene            4687..4851
                     /locus_tag="PL152_03630"
     CDS             4687..4851
                     /locus_tag="PL152_03630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48399.1"
                     /translation="MERFLKWLDDWDDLLGMLRVQARPIVVTLLLLGVFLAVAGAVLV
                     FGAPELLASP"
     gene            4862..5269
                     /gene="msrB"
                     /locus_tag="PL152_03635"
     CDS             4862..5269
                     /gene="msrB"
                     /locus_tag="PL152_03635"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HQR48400.1"
                     /translation="MNDREIPDRGELRGRLTDEQWRITQEKGTERPFTGRYLHNPEQG
                     VYSCVCCGQELFTSQAKYDSGSGWPSFWLPLAGDRVAVHRDTSHGMIRQEVTCARCGA
                     HLGHVFDDGPQPSGLRYCVNSASLDFKAEEPPK"
     gene            complement(5278..5904)
                     /locus_tag="PL152_03640"
     CDS             complement(5278..5904)
                     /locus_tag="PL152_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HQR48401.1"
                     /translation="MQIMSTSFGHGQRIPDEYTFCAPDPAAHVRMSANRNPHLRWSGV
                     PSAAKSLVLLCVDPDVPTRPDDVNQEGRSVPASLPRADFHHWAMVDIPPTVVEIAAGA
                     CSNGITARGKQSPAGPAGSRQGINDYTSWFAGDSEMGGQYFGYDGPCPPWNDELLHHY
                     HFVLYATDLARCPVDGAFTGPQVAKAIAGHVLAEARLTGTYSLNPAVR"
     gene            5998..6660
                     /locus_tag="PL152_03645"
     CDS             5998..6660
                     /locus_tag="PL152_03645"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR48402.1"
                     /translation="MQSGFRPPVPQAATIPGPAGALEARVEDPAAHVRPRAVGVVCHP
                     HPLHGGTMQNKVVHTLARAMQELGVPTVRFNFRGVGGSAGAYGDGVGEIEDARAVVAW
                     ARERWSCDALWLGGFSFGSAVALQLSVQIAPAALITVAPPVGRLLLDPVARPACPWLV
                     VQGERDELVDFATVRAWVAGYDPPPRFAAMREAEHFFHGRLGELRAVVSDFLSGIPAF
                     SG"
     gene            complement(6739..7050)
                     /locus_tag="PL152_03650"
     CDS             complement(6739..7050)
                     /locus_tag="PL152_03650"
                     /inference="COORDINATES: protein motif:HMM:NF012442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQR48403.1"
                     /translation="MAKKSSAPTKSDILAAISKDTGLSKKQVGAVFDSLATVIKKSLK
                     AHGLFTLPGLAKMKVVKKPATKAREGINPFTGEKMTFKAKPASKKVRVMPLKNLKGMV
                     N"
     gene            complement(7172..7666)
                     /locus_tag="PL152_03655"
     CDS             complement(7172..7666)
                     /locus_tag="PL152_03655"
                     /inference="COORDINATES: protein motif:HMM:NF025822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4426 domain-containing protein"
                     /protein_id="HQR48404.1"
                     /translation="MSSAARILPLLLACTMAAIAGCGQGGPDQGTAQTAAPSEPTSRD
                     FGDFQLHYNAVRSDVLTPDVARAYGIQRSANRVLLNVSVLKKNADGTTTPVDAAVTAT
                     AYNLNGQLKELTMRRITEGSSVYFIGEVAISGNEILVFDISAIPAGQASKYSVQFKRE
                     FFAG"
     gene            complement(7746..8042)
                     /locus_tag="PL152_03660"
     CDS             complement(7746..8042)
                     /locus_tag="PL152_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 family protein"
                     /protein_id="HQR48405.1"
                     /translation="MATSRRDGADLVLEVRVQPRAARSEFAGTFGDRVKIRLNAPPVD
                     GRANDALVTFLAAEFGVPRARVTIEHGLAGRDKRVRVRDVGPLAPGLRAIVGCD"
     gene            complement(8051..8575)
                     /locus_tag="PL152_03665"
     CDS             complement(8051..8575)
                     /locus_tag="PL152_03665"
                     /inference="COORDINATES: protein motif:HMM:NF014389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HQR48406.1"
                     /translation="MAAILFILDTLVTLVVVAFLLRVILPLVRADFRNPLGQAVLRFT
                     GPVVLPLRRVLKPVGRFDVASIVALVLVQLVGTALLRLAAGGGFAPVDMLVVGALSLA
                     QTVLQFYSVAVILYALLSWIAPGTASPATRLLDAICEPLLGPVRRVLPPVAGLDLSPL
                     FVVIALQALLILLR"
     gene            complement(8581..9399)
                     /gene="proC"
                     /locus_tag="PL152_03670"
     CDS             complement(8581..9399)
                     /gene="proC"
                     /locus_tag="PL152_03670"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HQR48407.1"
                     /translation="MRIAFIGGGHMSTALIGGLLRHGAPVNSIRVADPVPAQLERLAY
                     EFGVQGASDNRTAVAQADLVVLAVKPQDMPQAAREIAPGLATGRHLVVSVAAGVRLAD
                     LARWLGRSIPTVRAMPNRPALIGAGITALHAGPGVTADDRRAVERLMAAAGETIWLDD
                     ESQMDAATAISGSGPAYFFLLVEALEAAGIALGLPHETARRLAAETARGAGRMAAESG
                     VEPAVLREQVTSKGGTTAAALAVFEAGGMRDTVRRAVEAAARRSAELAVQSGGA"
     gene            complement(9415..10110)
                     /locus_tag="PL152_03675"
     CDS             complement(9415..10110)
                     /locus_tag="PL152_03675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HQR48408.1"
                     /translation="MLTVPQFLPDRLAAVTARIAAAAGRAGRDPAEVRLLAVSKQQPA
                     EMVRAAAAAGQREFGENYVQEAIPKIDSLRDLPLTWHFIGQLQANKTRDVAAACQWVH
                     TVDRPRIAERLSAQRPHYAGPLDVLLQVKVSGKAGKGGVAPESVADLAAVVTGLPRLR
                     LRGLMCMPEPAAGEMAQREPFRRLRELHDDLRRQGHALDTLSMGMSDDLEAAVLEGAT
                     IVRVGTAVFGPRN"
     gene            10199..11239
                     /locus_tag="PL152_03680"
     CDS             10199..11239
                     /locus_tag="PL152_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HQR48409.1"
                     /translation="MAVDIAQLLAFAVKNKASDLHLSAGVPPMIRVDGDMKRINMPPL
                     THKEVHSMVYDIMNDKQRKAYEEFYETDFSFEIPKLARFRVNAFNQNRGAAAVFRNIP
                     SVVLSLDDLNAPKIFKDIAMQPRGLVLVTGPTGSGKSTTLAAMINYVNDNRPDHIITI
                     EDPIEFVHESKRCLINQREVHRDTLGFSEALRSALREDPDVILVGEMRDLETIRLALT
                     GAETGHLVFGTLHTSSAAKTIDRVVDVFPAAEKEMVRAMLSESLRAVISQTLLKRIGG
                     GRIAAHEIMIGTPAIRNLIREGKIAQMYSAIQTGQAAGMQTLDQCLKELLQKGLITKE
                     EARFRAQNKEDF"
     gene            11276..12421
                     /locus_tag="PL152_03685"
     CDS             11276..12421
                     /locus_tag="PL152_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQR48410.1"
                     /translation="MDRDQGIKLMQDLLRRMVERKGSDLFITAGFPPAIKIDGEIKPQ
                     TNQPLTPEQSQLLVRAIMNDRQTKEFDATKECNFAIAPPSIGRFRVSAFVQMGLTGAV
                     LRTINVKVPTIDELELPPILKEIVMSKRGLVILVGATGSGKSTSLAAMLDYRNDKTAG
                     HIITIEDPIEYVHTHKNCVVTQREVGVDTDSWQMALKNTLRQAPDVILIGEIRDRDTM
                     EYGIQFAETGHLVLATLHANSANQAMDRIINFFPEDRREQLLMDLSLNIRAMIAQRLI
                     PREAGKGRIAAMEVMLNTPLIADLIFRGEVRAIKEIMAKSNRLGMKTFDQALYDLYET
                     GLISYEEALRNADSKNEIRLRIKLESKRADRGHLDETGKLKIVEEEE"
     gene            12432..13580
                     /locus_tag="PL152_03690"
     CDS             12432..13580
                     /locus_tag="PL152_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HQR48411.1"
                     /translation="MTATDIDSTNTSAERVLSDSTSGADSDRNFVLNTRPLFKLMVAK
                     KASDLFFTANSPIKIKIEGRIMPINRQVLTAEQVRQAAYGLMNQEQIDYFQQELEIDF
                     AISEPGLGRFRVAVFHQRGNPAMVLRYITAEMPTFEELGLPPIMKEMVMIKRGLVLMV
                     GATGSGKSTSLAAMINHRNLNSSDHILTVEDPIEFLHTNKKSIVNQREVGLDTKSFHR
                     ALRSAMRAAPDVILIGEIRDRESMEYAITFAGTGHLCLATLHANNAPEALDRIINMFP
                     RDQHSQIFLDLSQYLKAILSQRLVMGKEKKRCAAVEVLLNTPHVQELIKVGDVIGVKE
                     ALVRSAEKGMQSFDVALYQLYQDGKVTLEEALQNADSRANLEAKINFG"
     gene            complement(13616..14527)
                     /locus_tag="PL152_03695"
     CDS             complement(13616..14527)
                     /locus_tag="PL152_03695"
                     /inference="COORDINATES: protein motif:HMM:NF021903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HQR48412.1"
                     /translation="MSGREHRGTIFVEDAELLATQEHPGRQFVVRLRAPRCAAAATPG
                     SFVHLTCDPQIPMRRPLSIMRVDPAAGWIDLLFKIVGPGLEALSKRPAGATIPVLGPI
                     GKGFTPSPARPRTLLVGGGVGIPPMVFLAESLQARDDAHWQPLVLMGSEIPFPFRTRP
                     STILVPGMPDGTIACMPLLDDWGVPSRLATMAGFAGCYHGFVTDLAAEWLGSLDAAAL
                     GEVEMFACGPTPMLQSAAKVARRFGVPCQVSLEEFMACAVGGCAGCAVPVVTGTGLVI
                     KRVCVDGPVFDANAVYPGTFSYFSDEK"
     gene            complement(14524..>15159)
                     /gene="pyrB"
                     /locus_tag="PL152_03700"
     CDS             complement(14524..>15159)
                     /gene="pyrB"
                     /locus_tag="PL152_03700"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012412.2"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQR48413.1"
                     /translation="EPGLPAYVAAHVAPHVSVLNAGEAHVSHPTQGLLDTLTIRQRKG
                     DLSALSVAIVGDILHSRVARSTGRALLTLGVGQLRLVSPASLMPAAGELAGASRHTSL
                     ADGIRDADVVMALRIQRERMPDADKLDADAFNREWGITAENLRRAKPDAIVMHPGPMN
                     RGVEITSEVADGPQSAIRQQVGNGVAVRMAVLATIVRNVQARNVEAGRRAV"
BASE COUNT         2506 a         5226 c         5200 g         2227 t
ORIGIN      
        1 tccggctcgg tcttgcgcag cgccgcggcg tgctcgaaca cgagcacgta gccggtcgcg
       61 gggcgccagc ccaggtccgc gaggcaatcg aagagcgcgt cccagttgtg gccgaaggtg
      121 gacggaaaat cgagcgcggc ggccacgcgg tcgagcagct cgctcttgct cctgcagtcg
      181 aagaggtcga tccgcgtcgt atcgaggcca agcgagcggg ccagcgcgca gaccgcggct
      241 gtcgcggagg ccgtcagtgg cgcgacgtgg accgggcccg cgccgaaggc tgcggcgcgt
      301 tcgttcacgg gtggatctcc acgcgtcgga acgaccggta gtggtcgtcg gtgtagtagt
      361 acgcctcggg cgggtcaccg ccggtgacga tgcggcgcgc gccgcgatcc gccgatccgg
      421 gcgtggggac cgtgtactcg cggtagtaac cagccggctg tgccggcaac agccgctcgc
      481 ggttctggaa cacgactccg tcgcgcgcat atgggaacgg accgccgcgc cggatggcct
      541 cgagcgtatc gacggcctca ggtggcagga aatccgggta gtgggcggat gcgacggagg
      601 aggccgctgc gccggccgaa tcgggcccgg tgccgtggtg gccggaccac cactgcatcc
      661 cggcgatgag cgcgagcgcg acgacgacga ttcgcagctt gcctgacatg gtggtacgtc
      721 gatgcggtgt ggccgggccc gccacccgga gagcttagtc cgccgctgac ttctgcttca
      781 ggcgctggtc cacggtggcc gccgcggcct cggcccagtt gtcgatgcgc gccttcagct
      841 ggtccagcgt gagcggcgtc ttggcattct tgagctgctg tcccggaccg tggcgcacga
      901 ccatccccag gggctcgccg gtgacgctgt cggtaatgat cgcctcgacc gacagcatca
      961 cgtcctggct gcgctggccg gccgcgcgca tcgcggtgga aaacaccagc gcggcaggga
     1021 cgaactcata gggcttcatg ccagtgctgc cgaccgcgac cgcggtgact gccacgcgca
     1081 ggcgagcgac gccagggccg ggctcggtca cctgcggcac ctcgcggagg gcgacctcgc
     1141 gcaccgagtg cgtgaggtag gtgaggatgt cgtcgagcgt ctgctgtggc acctgctcgg
     1201 tggcctcggg cgtcgggtag aacaccacgt cgtcgacgat catcgcgtgg taattctcgc
     1261 gggtgagctt cgggctcagc cagcgttcga gcttgttgcc ggcggcgctc ttggcgggct
     1321 tcagctgcga atagtcgggc aggaagcccg agtgcggcac gtcggcggac cacgcgggcg
     1381 atgcgaccat gaacgtcgcc accgcgacca gacccacccg gacccggcgt ccgccaccga
     1441 ggcggccgaa tacactcgtc attctcatgc gctgctccat agctcggctc ggccacggcg
     1501 cggcgcgctg tggggttgct cacattctat ccaaggccgg actcgcgagt cccgggccgg
     1561 tcacgtttcc cgccgcccgg cgcggctata atccgcaacc catgaagcac cgccgaccta
     1621 tcgcagcgct cgctgcctgc atgctcgccg cgacggccag cctcgccatg gcgcagggct
     1681 tcggttccat ctcgagcggc aagcgcatgc cagacagtga gccggcgcct gccgcagcgc
     1741 aggagacgcc tgtcgttgcg acggcgacac ctccgacgga gtcggagcct gcagccagtc
     1801 cggcggaatc gtcggcgagt gtggcgacgt cgcccgaggt tacgccgtca attgctgctc
     1861 cgcaggagtc ggctgctgta tcgaccgcgc cacctccagc gaccgaagct tcgagtcttc
     1921 ctgcaccgga acccgtggcg ccggtcgttc agccacagga atcgtccgcg tcgaacgcaa
     1981 ccgcggagga accggcagcg tcgggcgggc cggcacaggc gcctgcctcg tcggcctcaa
     2041 tgacgccggc tgcggtcgcc gaatccgagc caactcaggc agttccatca gctcaggtag
     2101 ccgcggccgc gccgccgcaa gcgttctcct cgcaatcgtc gatgccagcg gaggcggccc
     2161 gcgcgccagt caactgcgac gaagcgaatc cattcatcac gggctcggcc gccgtcgaat
     2221 tcgcgcgcgc gtgcgcggcg accaatccgt tcctgtcggg aacggggatc gccacggtcg
     2281 ccatgaatgc gccgtccggg tcgttcggct ccggcgcggg ccccgcgctc gccacgccgg
     2341 gtccgaccgc cgaagggcag ggcgccggct cggctccggc ggtcagcggc ggcacgatgc
     2401 cgttcggcgg cagctacgag gatgcgatcg cctccggccc gcgctcgctc gagacggccg
     2461 atcgcgtgct ggtgaagaag agcgagcgca tgctgtacct gatgcgcgcg gaccgggtca
     2521 tcgccgagta cccgatcaag ctcggactca atccgtacgg acccaagcgc aagcaaggcg
     2581 atttccgcac gcccgagggc gattaccggc tggtgcgccg caattcccac agcgatttct
     2641 tcctggcgct cgaggtctcg tatccgaacg aggccgacgt cgagcgcgcc cgcgagatgg
     2701 gcgccgatcc cggcgggctc atcatgatcc acggccagcc gaacacgccc cgcaagcccg
     2761 cgtcgtacta cgcgagcaaa gactggaccg acggctgcat cgcagtgacc aatgccgcga
     2821 tggtggacat ctggctgcgc accgacgtcg ggacgccgat cgagatccgc ccgtgatgca
     2881 tccggcggag gtggtcgtcg ccgcgggcga cgatcaggat tcggtcgacg cccgcgtcac
     2941 cccgaccggc agcctggaga acctctccca gcaggaagtc gaccgcctgc tcgactcgtc
     3001 gcgcggcggg ctgtatccgc tgttccgccg ctgctcgctc gcggtgctga acgcgggcgg
     3061 cgagaccgac gacgcgcgcg ccatcttcga gcgctatgcc gactacgaga tccgcatcgt
     3121 gcgccaggcc tggggcatca agctcgagct gcgcaatgcg ccggccggcg ccttcgtcga
     3181 cggccgcatg atccgcggca tccaggagca cctgttcgcg gtgctgcgcg acatcctcta
     3241 catccacggc gaagtgtacg agcacggcag cgtcgagctg acggacacgc aggggatcac
     3301 caacgcggtg taccgcatcc tgcgcaacgc gggcgtgctg agaccgcgcg tgcggccgga
     3361 catcgtcgtc tgctggggcg gccactcgat cggccgcgag gagtacgact acaccaagcg
     3421 cgtgggctac gagctgggac tgcgcggcct gaacgtgtgc acgggctgcg gcccgggcgc
     3481 gatgaagggc ccgatgaagg gcgccaccat cggccacgcc aagcagcgca tccacgacgg
     3541 gcgctacatc ggcatcaccg agcccggcat cgtcgcggcc gagccgccca atgcgatcgt
     3601 gaaccagctg gtgatcatgc cggacatcga gaagcggctc gaggcgttcg tgcgcaccgg
     3661 acacggcatc ctggtgttcc cgggcggcgc cggcacggcc gaggaaatcc tctacctgat
     3721 gggaatcctg ctcgacccgg cgaatgcaca gcagccgttc ccggtcgtgt tcacggcgcc
     3781 ggagtcgagc cgcgagtact tcgcgcagat cgacgcgttc ctggccgcga cgctgggtca
     3841 gcgcgtgcgc gaccgctacc gcatcgtcgt cggcgatccg gcggaagccg cgcgcgaaat
     3901 gcagcgcggc atggacgccg tgcgcgaatt ccggcggcgc gcgagcgacg cttacaacta
     3961 cagctggacg ctcgcgatcg accaggaact gcagcggccc ttcgagccga cgcacgaggc
     4021 gatggcgcaa ctcgacctcg accgcgagct gccgccgcac cggctcgccg cgcaactgcg
     4081 gcgggcgttc tccggcatcg tctgcggcaa cgtgaaggac cacggcatcc gcctgatcga
     4141 gcagcacggc cccttcgaga tccacggcga caaggaactg ctcgcgccgc tcgacgcgct
     4201 gctcgagtcg ttcgtgcgcc agcggcgcat gcggctggcg gccgattacc ggccctgcta
     4261 ccgcgtcgtc acctgacctc gcctaactgt gacccagtac cggacgtagc ccccggtcca
     4321 ccgtagcctg ccgtcagtcg agtggtgacc gccatgactg accataagaa tcccagcagc
     4381 gacccggccc cggccgagac ggcagaaccg tcggcgcccg ggcgccgctc caagttcgga
     4441 gcggccgccg acggctggga cgcctataac gactggctgg agcgcgtccg ggcccagcag
     4501 ggccggatct cgcggcaggc cgtggtggcc aagtcgctct acaccgtctc tagctaccgg
     4561 aactgggccg acaaggcccg ggacgccttc gacaagggca agtgaccccc tccgggcccg
     4621 gcgcggctac catatatatg agtgaggcat ccggcggggt cgattgagcc tgccggcctt
     4681 cgggtcatgg aacggttcct caagtggctg gacgactggg acgacctgct cggcatgctg
     4741 cgggtgcagg cgcgacccat cgtcgtgacc ctgttgctgc tcggggtgtt cctggcggtg
     4801 gccggtgccg tgctggtctt cggcgcaccc gaactgctcg cctcgccctg agggcgattg
     4861 catgaacgac cgggaaatcc ccgaccgcgg agaactgcgc gggcgcctga ccgacgaaca
     4921 gtggcgcatc acccaggaga agggcaccga gcgcccgttc accgggcgct acctgcacaa
     4981 cccggagcag ggcgtgtaca gctgcgtctg ctgcggacag gaactgttca cgtcccaggc
     5041 gaagtacgac tcggggtcgg gctggccgtc gttctggctg ccgctcgccg gcgaccgcgt
     5101 cgccgtgcac cgggacacga gccacgggat gatccgccag gaagtcacct gcgcgcgctg
     5161 tggtgcgcac ctgggccacg tcttcgacga cgggccgcag ccgagcggcc tgcgctactg
     5221 cgtcaattcc gcatcgctcg acttcaaggc agaggagcca ccgaagtaac gccgacgtca
     5281 ccgcaccgcg ggattcaggc tgtaggtccc ggtgagccgc gcctcggcga gcacgtggcc
     5341 ggcgatcgcc ttcgcgacct gcggtccggt gaaggcgccg tccacggggc aacgcgcgag
     5401 gtccgtcgcg tacagcacga agtgatagtg gtgcaggagc tcgtcgttcc acggcgggca
     5461 cgggccgtcg tagccgaaat actggccgcc catctcgctg tcgccggcga accacgacgt
     5521 gtagtcgttg atgccctggc gtgaaccggc cggtccggcg ggggactgct tgccgcgcgc
     5581 ggtgatcccg ttcgagcagg cgcccgctgc gatctcgacg accgtcggcg ggatgtccac
     5641 catcgcccag tggtgaaaat cggcccgcgg cagcgaggcc ggcacgctgc gtccttcctg
     5701 gttcacgtcg tcgggccggg tcggcacgtc gggatccacg cacagcagga ccagcgattt
     5761 cgcggccgag ggcacgccgc tccagcgcag gtggggattg cgattggcgc tcatgcgcac
     5821 gtgggccgca gggtcggggg cgcagaaggt gtactcgtcc gggatgcgct gtccgtggcc
     5881 gaaactcgtg gacatgatct gcatggttca cctccggtcc cggcggcggg accgaccgca
     5941 gtgagtacag gtccgattct ggcacactgc acgctcgccg atggcggagg tgtcttggtg
     6001 cagagtggat tcaggccgcc cgttccgcag gcggcgacga tcccggggcc ggcgggcgca
     6061 ctcgaagcgc gtgtcgagga cccggcggcg cacgtgcgac cacgggcggt cggcgtcgtg
     6121 tgccatccgc acccgctgca cggcggcacg atgcagaaca aggtcgtcca cacgctggca
     6181 cgcgcgatgc aggaactcgg ggtgccgacc gtgcgcttca actttcgcgg cgtcggcggc
     6241 agcgccggag cgtacggcga cggcgtggga gagatcgagg acgcgcgtgc cgtcgtcgcg
     6301 tgggcacgcg agcgctggag ttgcgacgcg ctctggctcg gcggcttctc gttcgggtca
     6361 gcggtggctc tgcagctgtc agtacagatt gcgccggcgg cgctcatcac ggtcgcaccg
     6421 cccgtcgggc gactgctgct cgatccggtc gcgcgtcccg cgtgcccgtg gctcgtggtg
     6481 cagggcgagc gcgacgaact ggtggatttc gcgaccgtgc gggcgtgggt cgcgggttac
     6541 gacccgccgc cccgcttcgc agcgatgcgc gaggcggagc acttcttcca cggcaggctc
     6601 ggcgagctgc gcgccgtggt cagcgatttt ctctcgggaa ttccagcctt ttcgggctga
     6661 ggccgactcc gcggacggga gtggcgaggg gcggaaccgg ggcggcggtg cgccgccccg
     6721 gtctgaaacc ggatcggttc agttcaccat gcccttcagg ttcttgagcg gcatgacgcg
     6781 aaccttcttg ctggccggct tggccttgaa ggtcatcttc tcgcccgtga acgggttgat
     6841 gccttcgcgc gccttggtgg cgggcttctt cacgaccttc atcttcgcga ggccggggag
     6901 cgtgaacagg ccgtgggcct tcaggctctt cttgatgacc gtcgcgagcg agtcgaacac
     6961 ggcaccgacc tgcttcttgg acaacccggt gtccttggaa atcgccgcaa ggatgtcgga
     7021 cttcgtcggt gcagaactct tcttagccat ttaagtctcc tgtggatgga tcgcgggccg
     7081 ctggccccgt aaatcttcgg cgcgcaggaa aatagcacaa gattccagcg tatttaagcg
     7141 ctcgcgcgcc ttttttttcc gtgccgcagc gtcagccggc gaagaactcg cgcttgaact
     7201 gcaccgagta cttcgacgcc tggcccgctg gaatggcgct gatgtcgaac accaggatct
     7261 cgttgccgct gatggcgacc tcgccgatga aatacaccga cgagccctcc gtgatgcgac
     7321 gcatcgtgag ttccttcagc tggccgttga gattgtacgc cgtcgcggtc accgcggcgt
     7381 cgacgggggt cgtcgtgccg tcggcgttct tcttcagcac cgagacattg agcagcacgc
     7441 ggttggcgct gcgctggatg ccgtacgcgc gggctacatc cggagtgaga acatcggagc
     7501 gcactgcgtt gtaatgcaac tgaaaatcgc cgaaatcacg cgaggtcggc tcgctgggcg
     7561 cggcggtctg cgcggtgccc tggtccggcc cgccctggcc gcagcccgcg atcgcggcca
     7621 tggtgcaggc gaggagcagc ggaagaatgc gcgcggcgct cgacatcgtc gtatcccctt
     7681 gtgatgccga tgatcgatgg tccaactata ttccaagacc tgccgcagcg ctgcgagttc
     7741 cggagtcagt cgcagccgac gatggcccgc agtcccggcg ccaggggccc gacgtcccgg
     7801 acgcgcaccc gcttgtcccg tccggcgagc ccgtgctcga tggtcacccg cgcgcgtggc
     7861 acgccgaatt ccgctgccag gaacgtgacc agcgcgtcgt tggcacgccc atccaccggc
     7921 ggcgcgttga gccggatctt cacgcggtcg ccgaacgtcc cggcaaactc gctccttgcc
     7981 gcgcgcggct ggacccgcac ctcgaggacc aggtcggcgc cgtcgcggcg ggaagtggcc
     8041 accgctcgcc tcagcgcagc aggatcagca acgcctgcag cgcaatcacc acgaacagcg
     8101 gcgacaggtc caggcccgcc acgggtggca ggaccctgcg gaccgggccg aggagcggct
     8161 cgcagatcgc gtcgagcagc ctggttgccg ggctggccgt gcctggcgcg atccagctca
     8221 gtagcgcgta caggatcacc gcgacgctgt agaactgcag gaccgtctgc gccagggaca
     8281 gggcgccgac gacgagcatg tccacgggcg cgaaaccgcc gccagcggcc agccgcagca
     8341 gcgccgtgcc caccagctgc acgagcacga gcgccacgat ggacgccacg tcgaagcgac
     8401 cgaccggctt caacacgcgg cgcagcggca gcaccaccgg gccggtgaag cgcagcaccg
     8461 cctgaccgag cggattgcgg aaatccgccc ggaccagcgg caggatcacc cgcagcagga
     8521 acgcgacgac gaccagtgtc accagtgtgt cgaggatgaa gaggatcgct gccatcggtt
     8581 ctaagctccg ccagactgga ccgcgagttc ggccgaacgc cgggcagcag cttcgaccgc
     8641 gcgtcgcaca gtgtcgcgca taccgcccgc ttcgaacacc gccagtgcag cggcggtggt
     8701 gccacccttg gaggtgacct gctcgcgcag caccgccggt tcgacgccgc tctcggcggc
     8761 catgcgaccg gcgccgcgtg ccgtctcggc ggcgaggcgc cgcgctgtct cgtgaggcag
     8821 gccgagcgcg atgcccgcgg cctcgagcgc ctcgaccagc aggaagaaat aggccgggcc
     8881 gctgccggag atcgcggtgg cggcgtccat ctgcgactcg tcgtcgagcc agatcgtctc
     8941 gccggccgcc gccatcagcc gctcgaccgc acgtcgatcg tcggccgtca cgcccggccc
     9001 ggcatggagg gcggtgatgc cggccccgat cagcgcggga cgattcggca tggcccgcac
     9061 ggtcgggatc gagcgtccga gccagcgcgc gagatcggcg aggcgcactc cagccgcgac
     9121 ggaaacgacg agatggcgac cggtggcgag gccgggcgca atctcgcgag cagcctgcgg
     9181 catgtcctgg ggcttgacgg cgagcaccac gaggtccgcc tgcgcgacgg ccgtgcgatt
     9241 gtccgaggcg ccctggacac cgaattcgta cgcgaggcgt tcgagctggg cgggaaccgg
     9301 atcggcgacc cgtatcgagt tcacgggcgc gccgtggcgc agcaggccgc caatcagggc
     9361 cgtgctcatg tgtccgccgc cgatgaaggc gatgcgcatg ccgaatcctc cggatcagtt
     9421 gcgcggcccg aagaccgccg tgccgacgcg cacgatcgtg gcgccttcca ggaccgccgc
     9481 ctcgaggtcg tcgctcatgc ccatggacag cgtgtcgagc gcatgtccct gccggcgcag
     9541 gtcgtcgtgc agttcacgca gccggcggaa tggctcgcgt tgtgccattt ctcccgccgc
     9601 tggctcgggc atgcacatga gtccgcgcag ccggaggcgc ggcaggccgg tcaccacggc
     9661 ggcgaggtcc gcgaccgact cgggcgcgac gccgcccttg ccggccttcc cggagacctt
     9721 gacctgcagc agcacgtcga gcggcccggc gtaatgcggg cgctgggcgc tcaggcgctc
     9781 ggcgatgcgc ggccggtcga cggtgtgcac ccactggcag gctgcggcga cgtcgcgcgt
     9841 cttgttcgcc tgcagctggc cgatgaagtg ccaggtgagg ggcaggtcgc gcagcgagtc
     9901 gatcttcggg atcgcctcct gcacgtaatt ctcgccgaac tcgcgttgcc cggccgcggc
     9961 cgcggcacgc accatctccg ccggctgttg cttgctcacc gccagcaggc gcacctccgc
    10021 cgggtcccgc ccggcccggc cggccgcggc cgcgatccgg gctgtgaccg cggccaggcg
    10081 atcgggcaag aactgcggaa cggttaacat attggggtcc gaggcactga tctatactgc
    10141 gatggcccgc gcggaccacc cggatttcga gccggggcgc gggcgagagg gagccaagat
    10201 ggccgtcgac atcgcccagc tgctggcatt tgccgtcaag aacaaggcct ccgacctgca
    10261 cctgtcggca ggcgtgccgc ccatgatccg cgtcgacggc gacatgaagc ggatcaacat
    10321 gccgccgctg acgcacaagg aagtgcacag catggtgtac gacatcatga atgacaagca
    10381 acggaaggcg tatgaagagt tctacgagac ggacttttcg ttcgagatcc cgaagctcgc
    10441 gcgcttccgc gtcaacgcgt tcaaccagaa ccgcggcgcc gccgccgtgt tccgcaacat
    10501 cccctcggtg gtgctgtcgc tcgacgacct gaacgcgccg aagatcttca aggacatcgc
    10561 gatgcagccg cgcggcctgg tgctcgtcac cggcccgacc ggctccggca agtccacgac
    10621 gctcgccgcg atgatcaact acgtcaacga caaccgcccc gaccacatca tcacgatcga
    10681 ggacccgatc gagttcgtgc acgagtcgaa gcgctgcctg atcaaccagc gcgaggtgca
    10741 ccgcgacacg ctcgggttca gcgaggcgct gcgctccgcc ctgcgcgagg acccggacgt
    10801 gatcctggtc ggcgaaatgc gcgacctgga gaccatccgc ctcgcgctga cgggcgcgga
    10861 gacgggccac ctggtgttcg gcacgctgca caccagttcg gcggccaaga ccatcgaccg
    10921 cgtggtcgac gtgttcccgg cggcggagaa ggagatggtg cgcgcgatgc tctccgagtc
    10981 gctgcgcgcg gtcatctcgc agacgctgct gaagcgcatc ggcggcggcc gcatcgcggc
    11041 gcacgagatc atgatcggca cccccgcgat ccgcaacctg atccgcgagg gcaagatcgc
    11101 gcagatgtac tcggcgatcc agaccggcca ggcggccggc atgcagacgc tcgaccagtg
    11161 cctgaaggag ctgctgcaga agggcctgat caccaaggaa gaggcgcgat tccgcgcgca
    11221 gaacaaggag gacttctgac cgccggctgc ggccgggcgg ctgggagctt aaatcatgga
    11281 ccgcgaccag ggcatcaagc tcatgcagga cctgctgcgc cgcatggtgg agcgcaaggg
    11341 gtcggacctg ttcatcaccg cggggttccc gccggcgatc aagatcgacg gcgagatcaa
    11401 gccgcagacc aaccagccgc tcacgcccga gcagtcgcag ctgctggtgc gcgcgatcat
    11461 gaacgatcgc cagaccaagg agttcgacgc caccaaggag tgcaacttcg ccatcgcgcc
    11521 gcccagcatc ggccgcttcc gcgtgagcgc gttcgtgcag atggggctca ccggcgcggt
    11581 gctgcgcacc atcaacgtca aggtgccgac gatcgacgag ctcgagctgc cgccgatcct
    11641 caaggaaatc gtcatgtcga agcgcggcct ggtgatcctg gtcggcgcca ccggctcggg
    11701 caagtccacc tcgctcgcgg cgatgctcga ctaccgcaac gacaagacgg cgggccacat
    11761 catcacgatc gaggacccga tcgagtacgt gcacacccac aagaactgcg tggtcacgca
    11821 gcgcgaggtg ggggtggaca ccgacagctg gcagatggcg ctcaagaaca cgctgcgcca
    11881 ggcgccggac gtgatcctga tcggcgaaat ccgcgaccgc gacaccatgg agtacgggat
    11941 ccagttcgcc gagacggggc acctcgtgct cgcgacgctg cacgccaaca gcgccaacca
    12001 ggcgatggac cgcatcatca acttcttccc cgaggaccgg cgcgagcagc tgctcatgga
    12061 cctgtcgctc aacatccgcg ccatgatcgc gcagcggctg atcccgcgcg aggccggcaa
    12121 gggccgcatc gccgcgatgg aggtcatgct caacacgccg ctgatcgccg acctgatctt
    12181 ccgcggcgag gtgagggcca tcaaggagat catggccaag tcgaaccggc tcggcatgaa
    12241 gaccttcgac caggcgctct acgacctgta cgagaccggc ctgatttcct acgaagaggc
    12301 actcaggaac gccgactcga agaacgagat ccggctgcgc atcaagctcg agagcaagcg
    12361 cgccgaccgg gggcacctcg acgagacggg caagctcaag atcgtggagg aagaggaata
    12421 ggaccgcacg catgacggcg accgacatcg acagcaccaa cacgtcggcc gagcgggtcc
    12481 tgagcgactc gacctcgggc gccgactccg accgcaattt cgtgctgaac acgcggccgc
    12541 tgttcaagct catggtcgcc aagaaggcgt ccgacctgtt cttcacggcg aactcgccga
    12601 tcaagatcaa gatcgagggc cgcatcatgc cgatcaaccg gcaggtgctc accgccgagc
    12661 aggtgcgcca ggccgcctac ggcctgatga accaggagca gatcgactac ttccagcagg
    12721 agctcgagat cgacttcgcg atctccgagc ccggcctcgg ccgcttccgc gtcgcggtgt
    12781 tccaccagcg cggcaacccg gcgatggtgc tgcgctacat cacggccgag atgccgacct
    12841 tcgaggaact cgggctgccg ccgatcatga aggaaatggt catgatcaag cgcgggctgg
    12901 tgctgatggt gggcgccacc gggtcgggca agtccacctc gctcgccgcg atgatcaacc
    12961 accgcaacct caattcgtcg gaccacatcc tcacggtcga ggacccgatc gagttcctgc
    13021 acacgaacaa gaagtcgatc gtcaaccagc gcgaggtcgg gctcgacacc aagtcgttcc
    13081 accgcgcgct gcggagcgcc atgcgtgccg ccccggacgt gatcctgatc ggcgagatcc
    13141 gcgaccgcga gtcgatggaa tacgcgatca ccttcgcggg caccgggcac ctgtgcctcg
    13201 cgacgctgca cgcgaacaac gccccggagg cgctggaccg catcatcaac atgttcccgc
    13261 gcgaccagca ctcgcagatc ttcctcgacc tgtcgcagta cctgaaggcg atcctttcgc
    13321 agcggctggt catgggcaag gagaagaagc gctgcgcggc cgtggaggtg ctgctgaaca
    13381 ccccgcacgt gcaggagctc atcaaggtcg gcgacgtgat cggcgtcaag gaggccctgg
    13441 tccgcagcgc ggagaagggc atgcagagct tcgacgtcgc gctctaccag ctctaccagg
    13501 acggcaaggt cacgctcgag gaggcgctgc agaacgccga ctcgcgcgcc aacctcgagg
    13561 ccaagatcaa cttcggctga caggtgacag gggacattct cctatttcca ttctcctatt
    13621 tctcgtccga gaaataggag aatgtccccg gatacacggc gttagcgtcg aacaccggcc
    13681 cgtccacgca cacgcgcttt atcacgagac cggtcccggt gacgaccggg acggcgcagc
    13741 cggcgcagcc gccgacggcg cacgccatga actcctcgag cgagacctgg cacggcacgc
    13801 cgaaccggcg cgcgaccttc gccgcggact gcagcatcgg cgtgggtccg cacgcgaaca
    13861 tctcgacctc gccgagcgcc gcagcgtcca gcgacccgag ccattcggcc gcgaggtcgg
    13921 tgacgaaccc gtggtagcag ccggcaaagc cggccatcgt cgcgagccgg ctcggcacgc
    13981 cccagtcgtc gagcagcggc atgcaagcga tcgtgccgtc cggcatgccc ggcacgagga
    14041 tcgtcgaggg ccgcgtgcgg aacgggaacg ggatctccga gcccatgagc acgagtggct
    14101 gccagtgcgc atcgtcgcgt gcctgcagtg actcggcgag gaacaccatc ggcgggatcc
    14161 cgacgccgcc gccgaccagc agcgttcgcg ggcgcgccgg ggagggcgtg aaccccttgc
    14221 cgatcgggcc cagcaccggg atcgtggcac ccgccggtcg tttcgagagc gcctcgaggc
    14281 ccgggcccac gatcttgaac aggaggtcga tccagccagc cgctggatcc accctcatga
    14341 tcgacaacgg acgccgcatg ggaatctgcg ggtcacaggt gaggtgcacg aagctgccgg
    14401 gcgtggccgc ggccgcgcac ctcggtgccc gcagccgcac gacgaactgc cggccggggt
    14461 gctcctgcgt cgcgagcagc tccgcgtcct cgacgaagat cgtcccgcgg tgttcccggc
    14521 cgctcatacg gcccggcgcc ctgcctcgac gttgcgcgcc tgcacgttgc gcacgatcgt
    14581 ggcaagcacg gccatgcgca cggcgacgcc gttgccgacc tgctggcgga tcgcggattg
    14641 cggcccgtcc gccacctcgc tcgtgatctc gacgccgcgg ttcatcgggc ccggatgcat
    14701 gacgatggcg tcgggcttcg cccgccgcag gttctccgcc gtgatgcccc actcgcgatt
    14761 gaacgcgtcg gcatcgagct tgtcggcgtc gggcatgcgc tcgcgctgga tgcgcagcgc
    14821 catcaccacg tcggcgtcgc ggatgccgtc cgcgagcgac gtgtgccggc tggcgccggc
    14881 cagctcgccg gcggctggca tgaggctcgc gggactcacg aggcgcagct ggcccacgcc
    14941 cagcgtgagc agcgcgcgtc ccgtggaccg cgccacgcgc gaatgcagga tgtccccgac
    15001 gatggcaacg ctcaaggccg agaggtctcc cttgcgctgg cggatggtga gcgtgtcgag
    15061 caggccctgc gtcgggtgcg agacgtgcgc ctcgccggca ttcagcacgc tcacgtgggg
    15121 cgcgacgtgc gcggccacgt aggcgggcag cccgggctc
//
LOCUS       DAOMFD010000036        14942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_898_length_14942_cov_26.966078, whole genome shotgun sequence.
ACCESSION   DAOMFD010000036 DAOMFD010000000
VERSION     DAOMFD010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_898_length_14942_cov_26.966078"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="PL152_03705"
     CDS             <1..199
                     /locus_tag="PL152_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HQR48414.1"
                     /translation="NPGSVILSGEMMLRYMGWYEAADAIIASMDRTIGQKTVTYDFAR
                     LMAGATEVKCSEFADAMIRNL"
     gene            234..1988
                     /gene="aceK"
                     /locus_tag="PL152_03710"
     CDS             234..1988
                     /gene="aceK"
                     /locus_tag="PL152_03710"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF002804.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008772 - [isocitrate dehydrogenase
                     (NADP+)] kinase activity [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HQR48415.1"
                     /translation="MNLKVAKARTRDELIRECAQALVDSFADYNADFRAITQRARQRF
                     EDRDWRGSQRDAVERIDLYRKYVAGAVEVMRHELGDDVHERAVWSSIKRRFAEIIDPV
                     PDSDFPKTFFSSVTRQTFGTVGVDPAVEFVALDMDPLGSVKTHVETKVYVNRGSVELL
                     VEELLADFRFRTPYRDFDLSVRRVSDEIKAALDAGGERRTIERLEVIKEVFYQMTRAY
                     LVGRISGRDWTLPFVVALRNCESGVLVDAIMLDEPSVSVLFSYTRSYFHVDLAHVGEV
                     VRFLKEILPRKWVSELYTVLGRAKQGKTERFRELFRHLQSSDDQFVLAPGEKGLVMVC
                     FTLPSFDVVFKVIRDNFPDQKNIQRQDVLAKYDLVFKHDRAGRLVDAQEYKLVKLPRA
                     RFAPDLLEEMLAETANTVHEEGDDLVIGHMYVERRMTPLNLYVRTATRQQAEKAVLDY
                     GQCIRDLAVTNIFPGDLLLKNFGVTRNERVIFYDYDELCLVTDCRFREIPEPSHEEDE
                     MRGETWYYVGDNDVFPETFIDFLGFDPGLKQVFLEAHREILTAEWWRGIQERLREGDL
                     LEVLPYHKHRVRVFSSMV"
     gene            1985..3469
                     /locus_tag="PL152_03715"
     CDS             1985..3469
                     /locus_tag="PL152_03715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQR48416.1"
                     /translation="MNTSTRPPLWLLALLLVLAFAFQGTRGIWEPDEGRYTSAGLNML
                     SSGDWVLPTIDGEHPHLTKPPLTYWALASSFGLLGRNEWAARLPGALAFVGTGLLLFG
                     LGRKFCPGKPWLPAVVYALSLAPFMAANVVSTDILLVFFETAAMYAFVRAWQPGGGID
                     RRWIRPMWLAWGLAFMTKGPPGLLPLAAVAGMLALRDRAALKRIFDPLGLLAFGVVAF
                     TWFAVVIAQDPSRLGYFVGYEVYDRVFTSAHDRNAEWYGALLVYGPVFLFGALPWWPL
                     ALAAAGGPRRAWHTLRARIEARDPDWWLLLWWLLLPLAVFCLARSRLQLYVTPLFVPL
                     ALILARPLAKSGKLGAARLAAVAGVTSVALITLKGIAGHVHTGRDARDMAAAIRKVVD
                     PRSIDGIVFVGMRPFYGLRLYLDLPIEGVQVGPKRGDYSQFVAAEDLCSELATGERNV
                     YAMKEGRSAGFGTAVQQCGGLTAIRIGRFNADGNKIALFSVGAR"
     gene            3702..6647
                     /locus_tag="PL152_03720"
     CDS             3702..6647
                     /locus_tag="PL152_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR48417.1"
                     /translation="MPAAVAADAPAPDQALEEIIVTGSRIPRADFESASPVVTIPQDR
                     FEQAPAPTLEATLYYLPQFAGGTNGTSNNDLDNPQGQAQVALRGLGPQATLVLVDGRR
                     LIPANGNGVPDVNVIPPSLVEHVELLTGGASAVYGSDALAGVMNIRLRHNYEGLEFAG
                     DWGQTGRGDGQTYTATLTAGTDFGDGRGAIMGSVAYSDRSLVTQSERKYSRVGLLWVG
                     PGGGQGPGGQFVGSGSNNILEGRYAAGSNRPSREAYSELFGTYGYTVCTEEQEGAPPS
                     ERNCLPYQRDLGFNSDGSLFAQGTGDPGTVANFRGVQDPLIYNDRRYQFNNAPYQALM
                     MPLDRASAFVNGNFRFSDAAELYAQGIYAGYTVTEQLAPTPIPTALMPASNPYIPPDL
                     KFLLDSRPNPAGLVALSKRTLETGLRDTDHNYSFYQVALGLRGTVFTGWDYNGYVQYG
                     RSDQQMTVKGNVDLEKFEELLAAPDGGVATCGGADPFGINSISPGCASYMSVTTRDTG
                     KTTQTTAEFSLSGSPVELPAGALNTVLGLFYRKDTYEQRPDPLEQEVVLPGNRPRIAG
                     FGTSWLPMSADDHNIDAYTEVLVPLLEGRPGAKSLKTVLGYRWSDYASAGTANTYKAE
                     LLYEPVDALLVRGSFQHAVRAPSLFEVYEPQVNEIAGYNPPDPCSVGSPQRTGPNGAA
                     VDALCVAQGIPADQLATFYDDSGEAPGFFGGNPDLRPEKGRTWTAGAVLNSPFALPAL
                     QHLQLSLDWWNIQIRDAITYYFAADFIPQCYDPKYNPDFSAGNKYCSWFARDAEAGGY
                     IVDAYELERNIAVVETSGVDMQVDWRLPAGPGEIGTTWLLTWLASYDFQGDPEVKPQS
                     WKGTGCCPTLPEWKWSLDTHYQVGGFGANVIWSYLGHIDGRWVDGQDHPSFQVPVRNY
                     VDLTATYAWDSGPLDGLTLRIGCTNVFDEQPPIFPAHGNANSDLAAYDALGRSLWVGV
                     NYAVRPGGN"
     gene            6793..8835
                     /locus_tag="PL152_03725"
     CDS             6793..8835
                     /locus_tag="PL152_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48418.1"
                     /translation="MTALIVAAAAGLPFSIAVAYLLGGQRGVPDSAAMPGTQVPLPKA
                     RLRHYADAVVIGTLVLVLAVLLVRQSDLAKPKPPAHPAIAVLPFENLSGDPKQEYFSD
                     GLAEETLDRLGRVPGLKVIARSSSFSFKGQNLDVQTIAGKLGVTTILEGSVRRDGKRL
                     KLSARLIDGATGQETWSGSYDREVTDVFKVQEELARAIVDAVIPAARGEVARQAPPAE
                     ADLSAYDLYLLARQSQWQRTPEAVQRAVSLLERSIDLDPNYAPAHAQLAIALHTLVAS
                     STQTLDRDANRNRRLAEAHRALALDPGLPEAHLALAMATEGQNVPYETLAAMVKRALD
                     LNPNYARARFMYGAYLERAGRRDEALIWTERTLQVDPLAGPARRNLVAELHARGEDRR
                     RDEELRRAWQLWDTDADALYSLGGRYLEDMDDPVHAAEAERRSIALSPPLPTPDAALT
                     LHRVLVTVGAYDEAARLLQSTDWKTRSPATLAYEQAFVAGSRADLAGLDAAIDSIRAL
                     PPEVTRTAALLFWLTVAGRYADAAALDDPNLSLAAQRGILTGLYAEQIDLAAIWSARA
                     TRTRLEEARSAITDWQHELRDQLASYPLYVDALMQMAALESMQGHDAAAIALLEKAFG
                     RSPTPYFFVPQMPWFTRLEGNPGYDRLLVNWGAARAAARAKVLQLLAEAPQQPASR"
     gene            9034..11871
                     /locus_tag="PL152_03730"
     CDS             9034..11871
                     /locus_tag="PL152_03730"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR48419.1"
                     /translation="MAAIPAAIAADAPSSDENLEEIIVTGSRIQQRLEDAPVPVIVVD
                     RDAIERASQDSIGKVLQQIPINTGFAQNTNVNNGGDGSTRLNLRGLGSERTVVLVNGR
                     RFVYGGNGGNTSVDVNMIPLSLVERVEVVGVGSSTIYGADAIGGVVNMITRDDYQGAE
                     LSGSYTTNDQNDGHVYDISFVAGQSSDRAFLVFGAELVDQHEVFQAARDYSKQTEFIT
                     ADGSIATAGTPAVPQGYFELNGPNALGLEPGAYTRVNGSGNPPMAADFRLFCWPEDVV
                     ADPQRCPSTDLYDYAPVNYLQTPSTREYLWLLGGYDVNDYLAFFVEALVHHRGSEQLL
                     ASTPFWSIDPTGGAYIFPDGEPGIPADNYYNPFGVDLRYPDVVLRRFVESEGRRFTED
                     VKTQWYVAGARGNIGDSTWNWEFSVDYGKNDTDVTTQGEFRADHLQLALGHSGLDAAG
                     NIVCSSEPVTGVVPDASIIPGCVPMNLFGGQGPDGTGTITKAMLDYTTATLTDQGYNE
                     QQIYELILRGDWGAVQDRPVRWAVGGSHRVESAAAKLDPAKISGVAGDTPTNLNDGGE
                     ITANELYAESIVPLLADMTAAQELNLTGGLRYSDFSSFGSVTTYQAGLMYRPFRAVTL
                     RGGYGTVFRAPPIVALYESTNSWANSYYDPCGHDPTPAQQAHCEAAGVPGGSYVQDEN
                     DQFVELFGGNRDLQPEEGDSASAGITISPPAIPGLDFSMDYWRTSLDDVITLEPDMQS
                     YLDACADTGNPAACNRIERRADGSIRLVDARHANGASLVASGYDFDLGYRMPLAGGEL
                     SSRVTASYLDQYDYVTVKGADTIHAAGTRRYPFGALPRWRGLGYLEYARGDWSASYQA
                     QFIGSMRECGQGNTNDFAFDGCRTIDDTWYQDVRLQFSMKPGPTLTLAINNLGNVEPP
                     RVNFGSGANTDPAAYQLLGRTYYVSLSYRIE"
     gene            11891..>12632
                     /locus_tag="PL152_03735"
     CDS             11891..>12632
                     /locus_tag="PL152_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48420.1"
                     /translation="MTTQPAKSATSASGSLLNRLRERGVVRVAASYAVIAWLLLQIAD
                     VTLEPLGLPKWVMTALVIAAAVGFPIALALAWFLELGEAGITIDVAPEGAPRPTTRGL
                     RHYADITVIGVLVIAVVILLVRQSDIGQPKPPESPAIAVLPFQNLSGDPGQEYFSDGL
                     AEETLDRLGRVPGLKVIARSSSFTFKGSKLDARAIAQKLGVTTILEGSVRRDGMRLKL
                     TARLIDGATGQQAWSGSFDREMTDVFDVQ"
     assembly_gap    12633..12732
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12733..13776
                     /locus_tag="PL152_03740"
     CDS             <12733..13776
                     /locus_tag="PL152_03740"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR48421.1"
                     /translation="ASAWHNYAVLLANTGRREEAIRATERALELDPRYPPAWANYVGG
                     LLTPGGRRYKDAVARAIRMIGDIPDALNFVGVATTQFGYPVETMQVALAKRRLKVPEV
                     AGAWIFAYRAWLPVDLDRAAAALPMVDAASSRDFPKMIRLYSEIEVAGLKYDWAALDR
                     LFGELRALLGDNDSSLRSTMAFWLALQGRYAEAAQSLALARPVPEIAPPPRLGGDTLI
                     GLIEPAELRIYRGTGLEQEARRRAQELLVQLRADRPAADSTCEWYGWMRYAGIAANEG
                     LKSEAVDALRNAMRCSDLPFGFRPELPWFKSLEGYRPYDELVQERERRVGRIRAELLA
                     LDPPGGSPGPSAD"
     gene            13930..>14942
                     /locus_tag="PL152_03745"
     CDS             13930..>14942
                     /locus_tag="PL152_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48422.1"
                     /translation="MAASYAVIAWLLLQIASVVFEPVGLPKWVMTALIIAAAVGFPIA
                     IALAWFLEIGEQGVAVDTAEGAPRPSARGLRHYADAVVIGVLLVAVVILAVRQSDLGR
                     PGPPANPAIAVLPFENLGGDPQQEYFADGLAQELLDRLGRVPGLTVIARTSSFSFKGK
                     DVDSKTIAERLGVTTLLEGSVRRVGNRLKLSADLVDGASGRQIWSGTFDREVTDVFQV
                     QEEIAAAVIEAIVPAARGETAVKAVAPTGDMNAYDLYLLGRAAQEARTGERLRDSVGY
                     LEKAIAVDPNFAKAHAALSRSLLLWTIFTNVPAPADAMQRAEVEAHDALALDVNSSEA
                     HAAL"
BASE COUNT         2534 a         5043 c         4822 g         2443 t
ORIGIN      
        1 caacccgggc tcggtgatcc tctccggcga gatgatgctg cgctacatgg gctggtacga
       61 ggccgccgac gcgatcatcg cttccatgga tcgcaccatc ggccagaaga ccgtcaccta
      121 cgacttcgcg cggctcatgg cgggcgcgac cgaggtgaag tgcagcgagt tcgccgacgc
      181 gatgatccgc aacctgtagg cagcgcaggc ccgggagcgg cgggaaccac agcttgaacc
      241 tcaaggtcgc caaggcgcgc acccgcgacg agctgatccg cgagtgcgcg caggcgctgg
      301 tcgactcgtt cgcggactac aacgcggatt tccgcgcgat cacgcaacgc gcccgccagc
      361 gcttcgagga ccgggactgg cgcggcagcc agcgtgacgc ggtcgagcgc atcgatctct
      421 accgcaagta cgtcgccggg gccgtcgagg tcatgcgcca cgagctgggc gacgacgtgc
      481 acgagcgcgc ggtgtggtcg agcatcaagc ggcgcttcgc cgagatcatc gatccggtcc
      541 cggacagcga cttcccgaag accttcttct cgtcggtgac gcgccagacg ttcggcaccg
      601 tgggcgtgga cccggcggtc gagttcgtcg cgctcgacat ggacccgctc ggcagcgtca
      661 agacccacgt cgagaccaag gtctacgtca accgcggctc ggtcgaactg ctggtcgagg
      721 aactgctggc cgacttccgc ttccgcaccc cgtaccgcga cttcgacctg agcgtccgtc
      781 gcgtgtccga cgagatcaag gccgccctcg acgcgggtgg cgaacgtcgc acgatcgagc
      841 gcctcgaggt catcaaggaa gtcttctacc agatgacccg cgcgtacctc gtcgggcgca
      901 tcagcgggcg cgactggacg ctgccgttcg tcgtcgccct gcgtaactgc gagagcggcg
      961 tgctggtcga cgcgatcatg ctcgacgagc ccagcgtgag cgtgctgttc agctacacgc
     1021 ggtcctattt ccacgtcgac ctcgcgcacg tgggcgaggt ggtcaggttt ctcaaggaga
     1081 tcctgccccg caagtgggtg agcgagctct acaccgtgct cggccgcgcc aagcagggca
     1141 agaccgagcg gttccgggaa ctgttccggc acctgcagag ctccgacgac cagttcgtgc
     1201 tggcgcccgg cgagaagggt ctcgtgatgg tgtgcttcac cctgccctcc ttcgacgtcg
     1261 tgttcaaggt catccgcgac aacttcccgg atcagaagaa catccagcgc caggacgtgc
     1321 tcgcgaagta cgacctcgtg ttcaagcacg accgcgccgg ccgcctcgtc gacgcgcagg
     1381 aatacaagct ggtcaagctg ccgcgggcgc gcttcgcgcc ggacctgctc gaggaaatgc
     1441 tcgccgagac ggccaacacc gtccacgagg aaggcgacga cctggtgatc gggcacatgt
     1501 acgtcgagcg tcgcatgacg ccgctcaacc tgtacgtgcg cacggcgacc cgccagcagg
     1561 cggagaaggc tgtcctcgac tacgggcagt gcatccgcga cctcgcggtc accaatatct
     1621 ttccgggcga cctgctgctc aagaatttcg gcgtgacgcg caacgagcgc gtcatcttct
     1681 acgactacga cgagctgtgc ctggtgaccg actgtcgctt ccgcgagatc ccggagccca
     1741 gccacgagga agacgagatg cggggcgaga cctggtacta cgtcggcgac aacgacgtgt
     1801 ttccggagac gttcatcgac tttctcggct tcgacccggg cctgaagcag gtgttcctcg
     1861 aggcgcaccg ggagatcctg actgccgagt ggtggcgcgg catccaggaa cggctgcgcg
     1921 agggcgacct gctcgaggtg ctgccgtatc acaagcaccg cgtgcgcgtg ttcagcagca
     1981 tggtatgaac acctcgacca ggccaccgct ctggctgctg gcactgctgc tggtgctggc
     2041 attcgcgttc caggggacgc gcggcatctg ggagccagat gaaggccggt atacctccgc
     2101 tggactgaac atgctgtcga gcggggactg ggtcctgccg accattgacg gcgagcaccc
     2161 gcacctgacc aagcccccgc tcacctactg ggcgctcgcg tcgagcttcg gcctgctcgg
     2221 gcgcaacgag tgggcggcgc gactgccggg agcactggcc ttcgtcggca cgggcctgct
     2281 cctctttggt ctcggccgga agttctgccc ggggaaaccc tggctgccgg ccgtcgtgta
     2341 tgcgctctcg ctcgcgccat tcatggccgc gaacgtcgtg tcgacggaca tcctgctcgt
     2401 gttcttcgag acagcggcca tgtacgcgtt cgtgcgcgcc tggcagccgg gaggcggcat
     2461 cgaccggcgc tggatccgtc cgatgtggct cgcctggggc ctcgcattca tgaccaaggg
     2521 cccgcccggg ctgctgccac tggcagcggt ggccgggatg ctcgcgctgc gcgaccgcgc
     2581 tgcgttgaag cgcatcttcg acccgctggg gctgctcgct ttcggcgtcg tcgcgttcac
     2641 gtggttcgcg gtcgtcatcg cgcaggaccc gtcgcggctc ggctacttcg tcggctacga
     2701 ggtgtacgac cgcgtgttca cctccgcgca cgaccgcaat gccgagtggt acggcgcgct
     2761 gctcgtctac ggaccggtgt tcctgttcgg cgcgctgccc tggtggccgc tggcgctcgc
     2821 ggccgccggc gggccgcggc gcgcgtggca cacgctgcgc gccaggatcg aggcgcgcga
     2881 ccccgattgg tggttgctgc tgtggtggct gctgctgccg ctcgcagtct tctgcctggc
     2941 gcggtcgcga ttacaactct acgtgacgcc cctgttcgtg ccgctcgcac tcatcctggc
     3001 gcgaccgctc gcgaagtccg ggaagctcgg cgcagcgagg ctcgccgccg tcgcgggcgt
     3061 cacgtcggtg gcgctgatca cgctcaaggg catcgccggg cacgtccaca ccggccggga
     3121 cgcacgcgac atggcggcgg cgatccgcaa ggtcgtggac ccgcgctcga tcgacggcat
     3181 cgtgttcgtc ggcatgcgtc cgttttacgg cctcaggctg tacctggacc tgcccatcga
     3241 gggagtgcag gtcgggccga agcgcggcga ctattcccag ttcgtggcgg cagaggacct
     3301 ctgctcggag ctggccaccg gtgaaagaaa cgtgtacgcg atgaaggaag gccgctccgc
     3361 gggcttcggc accgcggtcc agcagtgcgg cgggctgacc gcgatccgca tcggccgctt
     3421 caacgccgac ggcaacaaga tcgccctgtt ctccgtcggc gcgcgctgag acacgtccgc
     3481 ccgccatggg agccgctcgc cgacggcgag gcttcgcctg cggccgcgcc gatctgacgc
     3541 aatgcgatta agttgcgttc agtttccagc ggggctagcc ttggccaagc acgcgaacgc
     3601 gtgtccgctt ccgggaccgg aagcgcaggg ggagagccat gagcgcaacg gcccgaagat
     3661 ggccggcgat tgtggtggcg gcagcagcac ccgtggccgc catgcccgcc gctgttgccg
     3721 cggacgcgcc ggcccccgac caagcgctcg aggaaatcat cgtcaccggg tcgcggatcc
     3781 cccgcgcgga cttcgagtca gcgagtcccg tcgtcaccat tccgcaggac cggtttgagc
     3841 aagcgcctgc cccgaccctc gaggctacgc tctattactt gccccaattt gccggcggca
     3901 cgaacggcac gtccaacaac gacttggaca atccgcaggg ccaggcgcag gtcgcgcttc
     3961 ggggtcttgg cccccaggca acgctggtgc tcgtggacgg aaggcggctc attccggcga
     4021 acggcaatgg ggttcccgac gtcaacgtga tcccgccatc actcgtggaa cacgtcgagc
     4081 tcctcacggg cggagcttcc gcagtgtacg gcagcgacgc tctcgccggg gtcatgaata
     4141 ttcgcctgag gcacaactac gagggcctcg aatttgcggg cgactgggga cagacgggtc
     4201 gcggcgacgg ccagacgtac accgcaacgc tgacggcggg cacagacttc ggcgatgggc
     4261 gtggcgccat catggggtcg gtcgcctact ccgaccgctc cctcgtgaca cagtctgaac
     4321 ggaaatactc ccgggttggg ctgctgtggg tcggccccgg gggtggccag ggaccgggcg
     4381 gccagttcgt cgggagcggg tcgaacaaca ttctcgaagg gcggtacgcg gccgggagca
     4441 accgccccag ccgggaagca tattctgaac ttttcggcac ctacggttac accgtttgca
     4501 cggaggaaca ggagggcgcg cctccatcgg agcggaattg cctgccttat cagcgagatc
     4561 tcggcttcaa cagcgacggg tccctgttcg cgcaaggcac cggggatccc gggaccgtgg
     4621 ccaatttccg gggcgtgcag gatccactca tctacaacga ccgccgatac caattcaaca
     4681 acgcgcctta tcaggcgctg atgatgccac tcgaccgggc gtccgcgttc gtgaacggaa
     4741 acttccgatt cagcgatgca gccgaactct atgcacaggg gatatatgcg gggtacacgg
     4801 tgaccgagca gcttgccccg acaccgatac ccacggccct catgcccgcc tccaatccct
     4861 acatcccgcc cgacctcaag ttcctactcg actcacggcc gaatcctgcc gggctggtcg
     4921 cactgagtaa gcgcaccctc gagacgggcc tgcgcgatac ggaccacaat tacagcttct
     4981 atcaggtcgc gctggggctg cgtggcaccg tcttcacggg ctgggattac aacggatacg
     5041 tccagtacgg ccggagcgac cagcagatga ccgtcaaagg gaacgtcgac cttgagaaat
     5101 tcgaggaact gctggctgcg ccggacggtg gcgtcgcgac ttgcggcggc gctgatcctt
     5161 tcggtatcaa ttccatttcc cccgggtgcg cgagctacat gtccgtgacg acgagggaca
     5221 ctggcaagac cacgcagacg acggccgagt tctcgctgag cggctcgccc gtcgagttgc
     5281 cggctggcgc gctcaatacc gtgctgggtc tcttctaccg caaggacacg tacgaacagc
     5341 gcccggaccc actggagcag gaggtggtgt tgccgggcaa tcggccacga atcgccggat
     5401 ttggcaccag ctggctgccc atgagcgccg acgaccacaa catcgacgcg tacaccgagg
     5461 tgctggtgcc cctgctcgag ggccgacccg gtgccaagtc gctcaagacc gtgctcggct
     5521 atcgctggtc cgactatgcc tcggctggaa cggcgaacac gtacaaggcc gaactgctgt
     5581 acgagcccgt ggacgcattg ctcgtgcgcg ggtcgttcca gcatgccgtg cgcgcgccgt
     5641 cgctgttcga ggtctacgaa ccgcaggtga acgagattgc tggatacaac ccgcccgatc
     5701 cgtgcagtgt cgggagccct cagcgcacgg gcccgaacgg cgcagccgtg gacgcattgt
     5761 gcgtcgcaca gggaattccc gccgaccagc tggccacgtt ttacgacgac agcggcgagg
     5821 cgccgggatt cttcggtggc aaccccgacc tgaggccgga gaagggtcgg acgtggacgg
     5881 ccggcgcggt tctcaactcg ccgttcgcgt tgccggcgct ccagcacctg cagctgtcgc
     5941 tcgactggtg gaacatccag atcagggacg ccatcacgta ctactttgcc gccgatttca
     6001 tacctcagtg ctacgacccg aagtacaacc ccgacttctc cgcgggcaac aagtactgca
     6061 gctggttcgc gcgcgacgcg gaggctggtg gctacatcgt cgacgcatac gagctcgaga
     6121 ggaacatcgc ggtcgtcgag acctcgggcg tcgacatgca agtggattgg cgcctgcccg
     6181 caggccctgg cgagatcggt acgacgtggc tgctgacgtg gctcgcatcg tacgatttcc
     6241 agggcgaccc ggaggtcaag ccgcaatcct ggaaaggcac cggctgctgc ccgacgctgc
     6301 cggagtggaa atggagcctc gacacgcatt accaggtcgg cggcttcggg gccaatgtca
     6361 tctggtcgta cctcggccac atcgacggcc ggtgggtgga cggccaggac catcccagtt
     6421 tccaggtgcc ggtgcgcaat tacgtcgatc tcaccgcgac ctatgcatgg gattccggcc
     6481 cgcttgacgg gctgacgctg cgtatcggct gcacgaacgt attcgacgag cagccaccga
     6541 tctttccggc gcacggtaac gccaacagcg atcttgctgc gtacgacgcg ctcgggcgat
     6601 cgttatgggt gggcgtcaat tacgccgtcc ggcccggcgg gaactagcga cacgaacgca
     6661 ccagaaacag caacgagtcg cctgcgcaca cgcgttgcgc tgcgcgtcac cgcgagctac
     6721 gcgatcattg cgtggctgct gatccagatc gcttccgtcg tcttcgaccc gctcggcgtg
     6781 ccgaagtggt tgatgaccgc gctgatcgtc gcagcggcgg ccgggctccc gttctcgatt
     6841 gcggtggcat acttgctggg cggccagcgc ggcgttccag attccgccgc aatgcccggc
     6901 acgcaggtgc cgctcccaaa ggcgcgcctc cgccactatg ccgatgccgt cgtcatcggc
     6961 acgctggtgc tcgtgctggc cgtgctgctg gtccggcaat ccgatctcgc aaagccgaag
     7021 ccgcctgccc atcccgccat cgccgtgctg cccttcgaga acctcagcgg cgacccgaag
     7081 caggagtact tctccgacgg cctcgccgag gagaccctcg atcgcctcgg ccgcgtcccc
     7141 gggctcaagg tcatcgcccg ctcctcttcc ttcagcttca agggccagaa tctcgatgtg
     7201 cagacgatcg ccgggaagct cggcgtcacg accatcctcg agggcagcgt ccggcgtgac
     7261 ggcaaacgac tgaagctcag cgcgcgcctg atcgacggcg ccacaggaca ggagacgtgg
     7321 tcgggcagct acgaccggga agtcaccgac gtgttcaagg tccaggaaga actcgcccgc
     7381 gccatcgtcg atgccgtcat cccggccgcc cgtggcgagg tcgcccgaca ggctcctcct
     7441 gccgaggccg acctgtccgc ctacgacctt tacctcctgg cgaggcagag ccagtggcag
     7501 cggaccccgg aggccgtgca gcgcgccgtg agcctgctcg aacgatccat cgacctcgac
     7561 ccgaactacg ccccggcgca cgcgcagctc gcaatcgccc tgcacaccct cgtcgcatcc
     7621 tcgacccaga cgctggaccg ggatgccaac cgcaaccggc gcctggccga ggctcaccgg
     7681 gcgctcgcgc tcgatccagg actccctgaa gcccacctcg ccctcgcaat ggcgacggag
     7741 gggcagaacg tgccctatga gacgctggct gcgatggtca agcgcgcgct cgacctgaat
     7801 cccaactatg cacgcgcccg attcatgtac ggcgcctacc tcgagcgcgc tggccgtcgc
     7861 gacgaggcgc tgatatggac cgaaaggact ctgcaggtgg acccactcgc aggcccggcg
     7921 cgccgcaacc tggtcgcgga actgcacgcc cgaggtgagg atcgcaggcg tgacgaggag
     7981 ttgcgacggg catggcaact ctgggacacc gatgcggatg cgctgtattc gctgggcggg
     8041 cgctacctgg aagacatgga tgacccggtg catgcggccg aggctgagcg tcgatcgatc
     8101 gccttgtcgc caccgctgcc gacgcccgac gcagccctca cgctgcaccg ggtgctcgtg
     8161 accgtcggcg cgtacgacga ggcggcgcga ctgctgcagt ccacggactg gaagacgcgg
     8221 tctcccgcga cgcttgcgta cgagcaggca ttcgtcgcgg gatcgcgcgc cgatctggcc
     8281 ggtctcgacg cggccatcga ctccattcgg gcgttgccgc ccgaagttac gcgcaccgcg
     8341 gcactgctgt tctggctgac ggtcgccgga aggtacgcgg acgcggcggc gctcgatgac
     8401 cccaacctat ccctggccgc gcaacgcggg attctgacgg ggctatatgc cgagcagatc
     8461 gacctggctg caatctggag cgcacgcgcc acccgcacgc gactcgaaga ggcgcgaagc
     8521 gcgatcaccg actggcagca tgagctgcgg gaccaactcg caagctaccc cctgtacgtc
     8581 gacgccttga tgcagatggc ggcgctcgag tcgatgcaag gacacgacgc cgcggcgatc
     8641 gcgctcctcg agaaggcgtt cggtcggagc ccgaccccgt acttcttcgt tccgcagatg
     8701 ccgtggttca cccggctcga gggcaacccg ggctacgacc gactcctcgt gaattggggg
     8761 gccgcgcgtg cagcggcccg agccaaagtc ctgcaattgc tagccgaggc accgcaacaa
     8821 cctgcgagcc gataagcccg cgcgctacgg catttcgttc gcaaggacgt cagcgcagcc
     8881 caatgcgaat aggttgcgtt caggtctgcg cgtggctagt ctagatcccg cacgcgaacg
     8941 cgtgaccgct tccgggaccg gaagcgcagg gggagagcca tgagcgcaac ggcccgaaga
     9001 tggccggcga ttgtggtggc ggcagcgacg cccgtggccg ccatacccgc ggctattgcc
     9061 gccgatgccc cctcctccga cgaaaacctc gaggaaatca tcgtcacggg ttcgcgcatc
     9121 cagcagcgac tcgaagatgc gccggtcccg gtcatcgtcg tcgaccgcga cgcgatcgag
     9181 cgtgcttcgc aggactccat cggcaaggtt ttgcagcaaa tcccgatcaa cacggggttc
     9241 gcgcagaaca ccaatgtcaa caacggcggc gacggcagca cccgcctgaa cctgcgcggg
     9301 ctcggctccg agcgcacggt ggtgctggtc aacggccggc gcttcgtcta cggcggcaac
     9361 ggcggcaaca cgtcggtcga cgtcaacatg atcccgctgt cgctcgtcga gcgtgtcgag
     9421 gtggtggggg tcggttcctc gacgatttac ggcgccgatg ctatcggcgg cgtcgtgaac
     9481 atgatcacgc gcgacgacta ccagggtgcc gagttgagcg gcagctacac gaccaacgac
     9541 caaaacgacg gccacgtcta cgacatcagc ttcgtcgccg ggcagtccag cgaccgcgct
     9601 ttcctcgtct tcggcgccga gttagtcgac cagcacgagg tgttccaggc ggcgcgcgac
     9661 tactcgaagc agacggaatt cataacagcc gatggctcga ttgcgaccgc cgggacacca
     9721 gccgttcccc agggctactt cgaactcaac ggtcccaatg cgctcggact cgagcccggc
     9781 gcgtatacgc gcgtgaacgg ctccggcaac ccgccgatgg ccgcggactt ccgcctattc
     9841 tgctggccag aagacgtggt cgcagatccg cagagatgtc cctccaccga cctgtacgac
     9901 tacgcgccgg tcaattacct gcagacgccg tcgacgcgcg agtacctgtg gctgctcggc
     9961 ggctacgatg tcaacgacta cctggcgttc ttcgtcgagg cgctggtcca tcaccgcggc
    10021 tcggagcagc tgctcgcctc gacaccattc tggtcaatcg acccgaccgg aggtgcgtac
    10081 atctttccgg acggcgagcc cggcattcct gccgacaact actacaatcc gttcggcgtg
    10141 gacctccggt atccggatgt cgtcctgcgc cgcttcgtcg agagtgaagg ccggcgcttc
    10201 accgaggacg tcaagaccca gtggtacgtt gcgggtgcac gcgggaatat cggcgactcg
    10261 acgtggaact gggaattttc ggtcgactac gggaagaacg acaccgacgt cacgacccag
    10321 ggtgagtttc gcgcggacca cctgcaactg gcgctcggcc actcgggcct cgacgcggcc
    10381 gggaacatcg tgtgcagttc ggaaccggtc accggcgttg tacccgatgc gagcatcatc
    10441 cccggctgcg tacccatgaa cctgttcggc gggcagggac ccgacggcac gggcaccatc
    10501 accaaggcga tgctggacta tacgacggcc acgttgaccg accagggcta caacgagcag
    10561 cagatctacg aactcatctt gcggggcgac tggggcgccg tccaggaccg ccccgtccgc
    10621 tgggcggtgg gcggatcaca tcgtgtcgag tccgcggctg ccaagctcga tcctgcgaag
    10681 atatcgggcg tagcgggcga caccccgacc aacctgaacg atggtggaga gatcaccgcc
    10741 aatgagcttt acgccgagtc gatcgtgccg ttgctcgccg acatgacggc cgcgcaggag
    10801 cttaacctca ccggcgggct gcgctactcc gacttctcgt cgttcggttc cgtcacgacg
    10861 taccaggccg gactgatgta ccggccgttc cgcgcggtga ccctgcgcgg cggctacggc
    10921 acggtatttc gcgcgccgcc gatcgtcgca ctctacgaaa gcacgaactc atgggcgaac
    10981 tcctactacg acccatgcgg ccacgacccg accccggctc agcaagcaca ctgtgaagcg
    11041 gccggggtgc ctggcggaag ttacgtgcag gacgagaacg accagttcgt ggaactcttc
    11101 ggcggcaacc gagacttgca gcccgaggaa ggagactcgg cttcggccgg cattacgatc
    11161 tcgccgcccg cgattcccgg cctcgatttc agcatggact actggcggac ttcgctcgac
    11221 gacgtcatca cgctcgaacc ggacatgcag tcctacctcg atgcctgtgc cgataccggg
    11281 aatcccgccg cgtgcaatcg catcgagcgt cgcgccgatg gctccatcag gctggtcgac
    11341 gcccgccatg ccaacggcgc cagcctcgtc gcttccggct atgacttcga tctcgggtac
    11401 cgcatgcccc tggcgggcgg cgaattgagc agccgggtca cggcctcgta tctcgaccag
    11461 tacgactacg tgacggtcaa gggcgcggac acgatccacg ccgccggcac ccgccgctat
    11521 ccgtttgggg ccttacccag atggcgaggg ctagggtacc tcgaatacgc gcgaggcgac
    11581 tggtcggcgt cgtaccaggc ccagttcatc ggctccatgc gggaatgtgg tcaaggcaat
    11641 accaatgact tcgcatttga cggctgccgc acgatcgacg acacctggta ccaggacgtg
    11701 cgcctgcagt tcagcatgaa acccggccca acgctgaccc ttgccatcaa caaccttggc
    11761 aacgtcgaac ctccgcgcgt caacttcggc tcgggggcca ataccgaccc ggctgcgtac
    11821 cagctcctcg ggcggaccta ctacgtgtcg ctgagctaca ggatcgagta acagacggaa
    11881 tgatcgccgg gtgacgacgc agccagcgaa atccgcaact tcggcatcgg ggtccctgct
    11941 gaatcgcctg cgcgaacgcg gcgtcgtgcg agtcgcggcg agctacgcag tcatcgcgtg
    12001 gctgctgctg cagatcgcgg atgtcactct cgagcccctg gggctgccca agtgggtgat
    12061 gacggcgctc gtcatcgcgg cagccgtggg atttccgatc gctctcgctc tcgcgtggtt
    12121 cctcgaactc ggcgaggcgg gcatcaccat cgacgtcgcg cccgagggtg ctccccgccc
    12181 caccacccgc ggattgcgcc attacgccga catcaccgtc atcggcgtac tggtgatcgc
    12241 tgtcgtgatc ctgctcgtgc ggcaatcgga catcggccag ccgaagccgc ccgagagtcc
    12301 tgccatcgcg gtactgcctt tccagaacct ctccggcgac cccgggcagg aatacttctc
    12361 cgacggcctc gccgaggaga cgctcgaccg cctcggtcgt gtgccggggc tgaaggtcat
    12421 cgcccgctcc tcgtcgttca ccttcaaggg cagcaagctc gacgccaggg ccatcgcaca
    12481 gaagctcggc gtgaccacca tcctcgaagg cagcgtgcgc cgcgacggaa tgcgactgaa
    12541 gctgaccgcc cgcctcatcg acggcgccac gggtcagcag gcctggtccg gcagcttcga
    12601 ccgcgagatg accgacgtgt tcgacgtgca ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12721 nnnnnnnnnn nngcgtcggc ctggcacaac tacgccgtcc tcctggccaa caccggccgc
    12781 cgtgaagagg ccattcgcgc aaccgagcga gccctcgaac tcgacccgcg atacccaccg
    12841 gcctgggcca actacgtggg tggccttctg acgccgggcg gcaggcgcta caaggacgcg
    12901 gtcgcacggg cgatccgaat gatcggcgac attccggacg cgctcaattt cgtcggcgtc
    12961 gcgaccaccc agttcggata cccggtcgag acgatgcagg ttgcactcgc taaacggcgc
    13021 ctgaaggtcc cggaggtggc cggcgcatgg atcttcgcgt accgcgcgtg gctcccggtc
    13081 gacctcgatc gtgcggccgc tgcactgccg atggtcgacg cggccagcag ccgcgatttt
    13141 cccaagatga tacgactcta ctccgagatc gaagtggccg ggctgaagta cgactgggct
    13201 gcgctcgacc gcctgttcgg tgagctccga gcgctgctcg gcgacaatga ctcgtccctc
    13261 cgatcgacca tggcattctg gcttgcgttg cagggccgat acgccgaggc cgcacaatcg
    13321 ctcgcactgg cccggccggt accggagata gcaccgccac cacgactggg cggcgatacg
    13381 ttgatcggcc tcatcgagcc ggcggagcta cgcatctatc gtggaacggg cctggagcag
    13441 gaagcccggc gtcgcgcgca ggaattgctg gtgcaactcc gggccgatcg gcctgccgcg
    13501 gattcgactt gcgaatggta tgggtggatg aggtatgccg gcatagcggc gaacgagggc
    13561 ctcaagagcg aggcggtcga tgccctgcgc aacgcgatgc ggtgttccga cctgccgttc
    13621 ggtttcaggc ccgagctgcc gtggttcaag tcgttggaag gctatcggcc ctatgacgaa
    13681 ctggtgcagg aacgcgaacg ccgcgtgggc cggattcgtg ccgaactgct cgcgctcgat
    13741 ccacccggcg gctcgcccgg cccgtccgca gactgacgcc gtcggcgacc cgcgacgccc
    13801 cgaaaggcta tccttgccgc cgtgacgcag acacccggag cattgccacc cgagcgcgga
    13861 agcccgcgaa cggcacccgc gagcgacggt catctgctcc accgcctccg cgaacgcggc
    13921 gtgctccgcg tggctgctag ttacgctgtg atcgcgtggc tcttgctgca gatcgcttcc
    13981 gtcgtcttcg agccggtcgg gctgccgaaa tgggtgatga cggcgctgat catcgccgca
    14041 gccgtcgggt ttccgatcgc gattgcactc gcctggttcc tcgagatcgg tgagcagggc
    14101 gtcgcggtcg ataccgccga gggtgcgccg aggccctcgg cgcgtggact ccgtcattac
    14161 gccgatgctg tagtgatcgg cgtcctgctg gtcgcggtcg tgatcctcgc cgtccgccag
    14221 tccgacctcg gcaggcctgg acccccggcg aatcccgcga tcgcggtgct gccattcgag
    14281 aatctgggtg gcgaccccca gcaggagtac ttcgccgacg gcctcgcaca ggagcttctc
    14341 gatcgcctgg ggcgggtccc gggactcacc gtcatcgccc ggacttcgtc gttctcgttc
    14401 aagggcaagg acgtcgactc gaagacgatc gcggaacggc tcggcgtcac gaccctgctc
    14461 gaaggcagcg tgcgtcgcgt cggcaaccgg ctcaagttga gcgccgacct cgtcgacggg
    14521 gcctcgggcc gccagatctg gtcggggacc ttcgaccggg aagtcaccga cgtgttccag
    14581 gtgcaggagg aaattgcggc agcggtcatc gaggcgatcg tgccggccgc gcgcggcgag
    14641 accgccgtga aggcggtggc accgaccggc gacatgaacg cctacgacct ctacctgctc
    14701 gggcgtgcag cccaggaggc gcgcaccggc gagcgactac gcgactccgt cggatacctc
    14761 gagaaggcga tcgccgtcga cccgaatttc gccaaggctc atgccgcctt gtcgcgatca
    14821 ctgcttctct ggacgatctt cacgaacgtg ccggctcctg cggatgcgat gcagcgcgcc
    14881 gaggttgaag cgcacgacgc cctggccctc gacgtgaact cctccgaggc ccatgcggca
    14941 ct
//
LOCUS       DAOMFD010000037        14622 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_932_length_14622_cov_33.568065, whole genome shotgun sequence.
ACCESSION   DAOMFD010000037 DAOMFD010000000
VERSION     DAOMFD010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14622)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14622
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_932_length_14622_cov_33.568065"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..757
                     /locus_tag="PL152_03750"
     CDS             <1..757
                     /locus_tag="PL152_03750"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HQR48423.1"
                     /translation="DWERYKEFKAPMHEASFEMIEIPHVRMSGKNSADIRMVVDALDL
                     CYTKSHVDTFVIVSGDSDFSPLVSKLRENNKLVIGVGVKSSTSDLLIANCDEFIYYDD
                     LVRKEEQRRGRRKAPAKQAAGKQSAPQAPAATAQAPTEAAASATGTTEPATADAPAAP
                     HAAKTEDEKKQEAFDLVLATIEALAEDREADEKIWGSMIKQALKRQRPGFNESYHGFR
                     SFSQLLEEAEARKLIGLEKDEKSGGYVVRAVAG"
     gene            complement(804..1175)
                     /locus_tag="PL152_03755"
     CDS             complement(804..1175)
                     /locus_tag="PL152_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48424.1"
                     /translation="MLNLLRFTYYLASAVSLFAGLATTASLFMADRAPQSPQYWVVTL
                     AVILFFGLAGLLLLCIGRQLVALGVLAGTATDDHGAILRARWNRLAACMVLGGLVLLP
                     ILLLVAYVIVARIEQGFAIFG"
     gene            1460..2623
                     /locus_tag="PL152_03760"
     CDS             1460..2623
                     /locus_tag="PL152_03760"
                     /inference="COORDINATES: protein motif:HMM:NF033577.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
                     /protein_id="HQR48425.1"
                     /translation="MDERLRFVARLFEGDKMATLCREFGISRKTGYKLYRRYRDVGFE
                     GLTDRSRRPYRQANKLPFQIEARIVELRKEHPSWGAPKIREKLRRLDLGVQTPAISTV
                     HAVLDRHGLVTRRRRRRHKAEGTPLSWPRQPNELWCADFKGEFMLADRRYCYPLTITD
                     FASRYLIACEALSSTRENTAFPVFERAFKELGLPGTIRTDNGIPFSCPHALFGLSRLS
                     VWWLRLGIDIERIKPGHPQQNGRHERMHLTLKKEATRPAADNLLQQQARFDEFIDEYN
                     RERPHQALGMRYPAELYVRSARPYRGLEELDYPFHDHTHLVTRCGRICLDNRKINLST
                     VFAGQKVGVKQVADQVWLVSFMHYDLGSFDNECCRVEAAENPFGVKVLPMSPE"
     gene            complement(2663..2739)
                     /locus_tag="PL152_03765"
     tRNA            complement(2663..2739)
                     /locus_tag="PL152_03765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2703..2705),aa:Arg,seq:ccg)
     gene            2874..3383
                     /locus_tag="PL152_03770"
     CDS             2874..3383
                     /locus_tag="PL152_03770"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR48426.1"
                     /translation="MSASHDRKFFDTFLLVLGILVGIALALIIVARIIASNTSAVRQQ
                     GDPVYEKQVEARIMPVARDAVAGQDNSALAAPPAAAPAAATDLAGDAVYNQVCTACHG
                     AGVAGAPKYGDKAAWAPRIAQGMDTLHQHALQGFQGKAGFMPPKGGRTDLTDQSVKNG
                     VDYMVAGSK"
     gene            3392..5182
                     /locus_tag="PL152_03775"
     CDS             3392..5182
                     /locus_tag="PL152_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family metallopeptidase"
                     /protein_id="HQR48427.1"
                     /translation="MARAALVALALSSLPVVAPAASAGQAGAGTPLSTGNLRSTGELS
                     AYTRTGRYDEVERLCRAYAARWPRQLRCAEFGRTPEGRPMLALVASADGTLSPAQARA
                     RHRPVLLFQGGIHAGEIDGKDAGLLALRELLDGNAQPGVLGKITVVFVPVYNVDGHER
                     FGRWHRPNQRGPEESGWRTTAQNLNLNRDYTKADAPETRAMLGLLDAWDPILYVDLHV
                     TDGAQFQPDISVQLEPCDGWDHELGIAGCAVRDAVLADLERQGYIALPFYPSFVRDDE
                     PESGFSVSVSNPRYSTSYWATRNRFSALVETHSWKPYARRVAATHAAILAMVGLAARD
                     GKTWLADASTADERASRLAGQPVALSYKNSDATRTIEFPGYAYSREPSPISGQSWIRY
                     DESSPQVWRVPLLYEVVPDLEIVAPAQGYVVPVAYAAWMSDRLKLHGIRYEVLDRPQP
                     QLAVQVFRADSVKQDDESFEGHVGVHVTGAWSDEARDVPAGSLFVPIAQPGARLVMSL
                     LEPQAPDSYVSWGYFDASFEQKEYMENYVAEAVARDMLARDAGLRAEFEQRLAADPAF
                     AADPDARLDFFRRRHPSWDDRYDLYPVYRR"
     gene            complement(5242..6600)
                     /locus_tag="PL152_03780"
     CDS             complement(5242..6600)
                     /locus_tag="PL152_03780"
                     /inference="COORDINATES: protein motif:HMM:NF025885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQR48428.1"
                     /translation="MSNARILVVDDEADIRELVQEILTEEGYAVSVSASAEEARAACA
                     VQQPDLVLLDIWMPGTDGISLLREWQQSYGSALPVVMMSGHGTVETAVEATRLGAIDY
                     VEKPLSLAKLLRTVRSALEEGDRRRRATRAMVPPLIAPAGRSRVMRELREQVKLLAAH
                     DAPVLIVGEPGTGREAFARYLHSLSPRSAGPLVMAVAGSLTADGAEAALYGSESGGTV
                     RKGLIEQAEHGTLFLNELGDLGPVAQRLLFAAIDAGEFRRVGGSTSVRFDTRIVSSAQ
                     PGFEGRGAEPFRVDLLSHLSVLMLRIPPLREYAEDVPDLLRHYVDRLVEDERLGFRRF
                     SVAAQNRLRNYPWPGNIRELKNLVHRLLIHGGPAEIGLEEIESEIAAQAAADEPLVKQ
                     DLLSMPLREAREHFERAYLTQQLQLCGGKVGQLAKRVGMERTHLYRKLRSLGVDFRQT
                     ED"
     gene            complement(6597..8837)
                     /locus_tag="PL152_03785"
     CDS             complement(6597..8837)
                     /locus_tag="PL152_03785"
                     /inference="COORDINATES: protein motif:HMM:NF039586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR48429.1"
                     /translation="MAAPTLSRALSAALMGLGGLLWLAAILFMAQTAQNSDQFSRLHP
                     WILGINVAGLAVLVTLLATRIVQLVRDWRGQVIGSRLKARMVWTFGVLATLPILLVYF
                     FAVQFLNRGIDSWFNVEIRKSLDDALVLSRSALEARMREHLADTQMIADALANSGGTM
                     PGLLESLRQQVGALDIVVSTEAGTLMAVSKGLSSEVVPDEVPGEVLLQVRQGRPFVSL
                     EPEPEGGYLVRTAVPLLDTGPARDRLVLQAMYPVAERLGALADTVQAAYKQFGEKDYL
                     RRPLKASFILTLTVVLLLSLLAAWYGAIFTAQKLVQPIQDLVKGTRAVAAGDLDTRLP
                     LTSHDEMGFLVHSFNDMTKRLRRAREDQQRAQQAAETERANLAVILARLSTGVMSLEP
                     DLRVRTVNRAAGMILGEDLEPCVGLRLGELGETGQREGLLRQFVESCRARLGAGDLEW
                     REQLTFRAGTGRRIIMCACTPLPGEGEAPGGYVLVFDDITAMLQAQREAAWGEVARRL
                     AHEIKNPLTPIRLSAERMRLKLMPSMSEKDAQLLDRGTETIVQQVEAMKEMVNAFSEY
                     ARAPRFEMAPVGLNKLVTEVTDLYRAQVTGRGVRMEVQLDPSLGTVVADQGRLRQLLH
                     NLLTNAVEALEGHPDGGIRVSTRLADSGGAGIAEVVVEDNGPGFPRELVGQVFDPYVT
                     TKARGTGLGLAIVRKIVEEHGGSIEADNRPDGGARVRIGLPVSDTSGVQARREARRAE
                     PRRERA"
     gene            complement(8819..9361)
                     /locus_tag="PL152_03790"
     CDS             complement(8819..9361)
                     /locus_tag="PL152_03790"
                     /inference="COORDINATES: protein motif:HMM:NF025692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4390 domain-containing protein"
                     /protein_id="HQR48430.1"
                     /translation="MLLACFLATVAATAAVAQTQVNVRSASLSLDQDVYELDARIEVR
                     LPDDARKAIEAGLTLRLDYEIELNRVRNYLPDATVASLVQSYELSYHALSQRYLLRNL
                     NTGEQDDFGTLVAAMDRLCEVRGLPVVDATLVQPGPSYEAAVRAVVDLNTGSEALRWI
                     LFWTDDWSAKSEWYEWPLRP"
     gene            complement(9430..10758)
                     /gene="rsmB"
                     /locus_tag="PL152_03795"
     CDS             complement(9430..10758)
                     /gene="rsmB"
                     /locus_tag="PL152_03795"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: protein motif:HMM:NF008149.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HQR48431.1"
                     /translation="MAAEPSAAEIRAAAARVVSQTVDRGRSLDEQLAASRESGAARGL
                     KHSLCYGTLRWHFRLAAVLRALCDQPPGHLKPELRALLEVGLFQLMSGEVAEHAAIAE
                     TVEAARALGFAKASGFVNAVLRRFQRERETVLAAVDAEPAVRTAHPRWFVDALGRECP
                     DAREAILEANNARPPMWLRVNRMRTDVAACETDLRARGWDVHRHPFAPDALRIAPPVD
                     VHSLPGFGEGRVSVQDAAAQLAVELLAPQPGERILDACAAPGGKTCHLLERVNGVADV
                     TALDVSPQRLERVRDNLRRLGLTAQVLAGDVGDHRSWWDGRPYDRVLLDAPCSATGVI
                     RRHPDIKVLRRARDIPALARRQASLLLAAWGLVKPGGTLLYTTCSALRAEGEDVVAGF
                     LAGTADAADGTGERCRGWPPAPAGTGPGYRVHAGQADMDGFYYACLTRRP"
     gene            complement(10758..11756)
                     /gene="fmt"
                     /locus_tag="PL152_03800"
     CDS             complement(10758..11756)
                     /gene="fmt"
                     /locus_tag="PL152_03800"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HQR48432.1"
                     /translation="MNHRLRIVFAGTPDFSVPALDALHGAGHGIAAVYTQPDRPSGRG
                     REVTAGPVKRRALELGLTVEQPATLRTDDAVARLASFMPDVMVVAAYGLILPQAVLDV
                     PRRGCLNIHASLLPRWRGAAPIQRAILAGDARTGITIMKMDAGLDTGPMLLSREITIA
                     PGESAGSLHDRLAVLGAEAIVAAVGAWADGRLGAVPQPAEGATYAPKLRKEEALIDWS
                     RPAVEIERQVRAFDPWPVAETRWMGRQLRIWEAAAVAITSGDSFRVAADTAAGLVDTG
                     GGDAAPGCVLQASHGRLLVATGAGVLEVRRLQLAGRSAMPVAEFLNAHALAGATLG"
     gene            complement(11800..12345)
                     /gene="def"
                     /locus_tag="PL152_03805"
     CDS             complement(11800..12345)
                     /gene="def"
                     /locus_tag="PL152_03805"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009682343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HQR48433.1"
                     /translation="MAKLTILEFPDPRLRTRAQPVAVVDDGIRRLIDDMLETMYAAPG
                     VGLAATQVDVHKRVLVADVTKEQDHPLAFVNPEIIEREGTVEAEEGCLSVPGIFDTLN
                     TRSARIVVRALGRDGKPFEMEADGLLAICIQHEMDHLEGKLFVDYLSDLKRTRIRKKL
                     EKERREQGSAGRRERATAPVI"
     gene            12523..13731
                     /locus_tag="PL152_03810"
     CDS             12523..13731
                     /locus_tag="PL152_03810"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HQR48434.1"
                     /translation="MDRKLRVAPRISTRLSHWPRALGMALAVAGVGLALGVTLPVPAA
                     AQEASGHAMSVPLAPDAPDTYVVKKGDTLWDIAGVFLKYPWYWPEIWYVNPQVANPHW
                     IYPGDVLHLIYVDGKPRVTVGTAGEVRLSPQVRTDPLGQAIRTVPYDVLMNFVQHPSL
                     LTEKQVDKAPYIVGFRGGHLVGAGVNEAYATGLDNPPVGNEYTVVHVGTELRDPDDGD
                     LLGYIGHYAATTRVIATTDAKHEDPITHLAVLDNGREIQQGDKVLPAINKFGDDFVLS
                     APSDRKLNGQVLAVVNGLAVGGTYQVLAINRGKRDGLAPGNIVAIFARGLDVANYYSR
                     GQDWTSFTANYSKVKLPDERSATMLLFTVYDRMSYGLVVESTQPVHIGDFIKHPDFGH
                     DDLGMPATAR"
     gene            13808..>14622
                     /gene="dprA"
                     /locus_tag="PL152_03815"
     CDS             13808..>14622
                     /gene="dprA"
                     /locus_tag="PL152_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HQR48435.1"
                     /translation="MDDPKPWLTLARAPGLHAGHLRDPEGASIPPLALLREPARSLGS
                     LGLGAAMVEAIRNPSTAGLEADERWLAGSGRTLVTWGSPEYPALLATIPDAPLVLFVA
                     GAAASLHLPQLAVVGSRNPTAIGRDTAEDFARHLAAAGFAITSGLALGIDAAAHRGAL
                     AGRGLTIAVLGCGLDRVYPRENAALAEQILAHGALVSDLPAGTPPLKQHFPRRNRIIS
                     GLAVGTLVVEAALQSGSLITARLAAEQGREVFAIPGSIHSPLSRGCHRLIRQGA"
BASE COUNT         2289 a         5276 c         4877 g         2180 t
ORIGIN      
        1 cgactgggag cgctacaagg aattcaaggc gccgatgcac gaggcctcgt tcgagatgat
       61 cgagatcccg cacgtgcgca tgtcgggcaa gaactccgcc gacatccgca tggtggtcga
      121 cgcgctcgac ctctgctaca ccaagtcgca cgtcgacacg ttcgtgatcg tgagcggcga
      181 ctccgacttc tcgccgctgg tgagcaagct gcgcgagaac aacaagctcg tgatcggcgt
      241 gggcgtgaag agctcgacgt ccgacctgct gatcgccaac tgcgacgagt tcatctacta
      301 cgacgacctc gtgcgcaagg aagagcagcg gcgcgggcgc cgcaaggcgc cggcgaagca
      361 ggccgcgggc aagcagtccg cgccccaggc gccggccgcc acggcacagg cacccaccga
      421 ggccgccgcg tccgcgacgg gcacgacaga accggcaacc gcggacgcgc cggccgcgcc
      481 gcatgccgcg aagaccgagg atgagaagaa gcaggaggcc ttcgacctcg tgctcgccac
      541 catcgaggcg ctggccgagg accgcgaggc ggacgagaag atctggggct cgatgatcaa
      601 gcaggcgctg aagcgccagc gccccgggtt caatgagagc taccacggct ttcgcagctt
      661 cagccagctg ctcgaggagg ccgaggcccg caagctgatc ggcctcgaga aggacgagaa
      721 gtcgggcggg tatgtggtga gggccgtcgc gggctgagtt caccgacccg cgtgacgctg
      781 aaccgtcccg tgtttgaggc cgactaaccg aatatcgcga agccctgctc gatccgcgcg
      841 acgatcacgt aagcgacgag cagcaggatc ggcagcagta ccaggccgcc gagcaccatg
      901 caggccgcga gccgattcca gcgcgccctg agaatcgcgc cgtggtcgtc ggtggcggtt
      961 cctgccagga cacccagggc aaccagttgc cgtccgatac acagcagcag tagtccggcc
     1021 agtccgaaga acaggatgac cgccagagtc accacccagt actgcgggga ctggggagcc
     1081 cggtcggcca tgaacaggct ggccgtcgtc gccaggccag cgaaaaggct gacggcagag
     1141 gcgaggtaat aggtgaagcg aagcaggttc agcatggcct ggtacctgaa atcatccgac
     1201 aggattgcgg catcgctccg cttcatgccg ccccgctggg gccgtcgccg atgcgctcct
     1261 tcgccgcgaa tcggcaacgt cccaccgcgc tggcgcgcgg tggtcgaacc gtgtgacgac
     1321 gtcacaggtt ctcatgcctg tcggggtccc attcgcgatg cccttcctga cagaggggta
     1381 acagttcatt ccgggcacat aggtaacact ttcaggcctt tgcgggagac gcaaagtgcc
     1441 gtggcaggag tgttacaaga tggacgaacg gctacggttc gttgcccgac tgttcgaagg
     1501 cgacaagatg gccaccctct gccgggagtt cggcatctcc cgcaagaccg gctacaagct
     1561 ctaccgacgc taccgcgacg tcggcttcga gggcctgacc gatcgcagcc gccgacccta
     1621 ccggcaggcc aacaagctgc ccttccagat cgaggccagg atcgtcgagc tgcgcaagga
     1681 gcacccctcc tggggcgcgc ccaagatccg cgagaagctg cgtcggctcg acctcggcgt
     1741 gcagaccccg gcgatcagca ccgtccacgc cgtgctcgat cgacacggcc tggtcacccg
     1801 ccgccgtcgc cgacgccaca aggccgaggg aacacccctc tcctggccac gacagcccaa
     1861 cgagctgtgg tgcgccgact tcaagggcga gttcatgctc gccgaccgtc gctactgcta
     1921 cccgctcacc atcaccgact tcgccagccg ttacctgatt gcctgtgagg cgctctcgag
     1981 cacccgcgag aacaccgcat tccccgtgtt cgagcgggcc ttcaaggaac tcggcctgcc
     2041 gggcactatc cgcaccgaca acggcatccc cttctcctgc ccacacgccc tcttcggcct
     2101 gagccgcctc tccgtctggt ggctgcgcct cggcatcgac atcgaacgca tcaagcccgg
     2161 ccatccgcag cagaacggtc gccacgagcg catgcacctg accctgaaga aggaagccac
     2221 ccggcccgcc gccgacaacc tgctgcagca gcaagcccgc ttcgacgagt tcatcgacga
     2281 gtacaaccgc gagcgccccc accaggccct cggcatgcgc taccccgccg agctctacgt
     2341 gcgctcagcc cgcccctacc gcggcctcga ggaactcgac tacccgttcc acgaccacac
     2401 ccacctcgtc acccgctgcg ggcgcatctg cctcgacaac cgcaagatca acttgagcac
     2461 cgtcttcgcc ggacagaagg tcggcgtcaa acaggtcgcc gaccaggtct ggctggtcag
     2521 cttcatgcac tacgatctgg ggtccttcga caacgaatgt tgccgcgtgg aggccgccga
     2581 gaatccattc ggcgttaaag tgttaccgat gtccccggaa taatccgtta cccatgtgtc
     2641 cggtatggac ccccttccag aatggcgcgc ccgacaggat tcgaacctgt gacccctgcc
     2701 ttcggagggc agtactctat ccaactgagc tacgggcgcg cggccggacc ccgaaaaggg
     2761 gcgggaatca tagcctcggg gcggtcggcc gtccatttcg gggtggcgcg gcagtgcgcc
     2821 ctgcacggta tactgcgcgg cctcgttttc gtgaccagat ccggggaccc accgtgagcg
     2881 cttcgcacga ccgcaagttc ttcgacacct tcctgctggt gctcggcatc ctcgtcggta
     2941 tcgcactcgc gctgatcatc gtcgcgcgca tcatcgcgtc caatacgtcg gccgtgcggc
     3001 agcagggcga cccggtctac gagaagcagg tcgaggcccg catcatgccc gtcgctcgcg
     3061 acgccgtggc cggccaggac aactcggccc tcgcggctcc tcccgcagcc gcaccggcgg
     3121 cagccaccga cctcgcgggc gacgcggtct acaaccaggt ctgcacggcc tgccatggcg
     3181 caggcgtggc gggcgcgcca aagtacggtg acaaggcagc gtgggccccg cgcatcgcgc
     3241 agggcatgga cacgctgcac cagcacgcgc tgcagggctt ccagggcaag gctggcttca
     3301 tgccgcccaa gggtggccgc accgacctca ccgaccagtc ggttaagaac ggtgtcgact
     3361 acatggtggc cggatccaag tgatcccggc cgtggcgagg gcggcgctcg tcgccctcgc
     3421 gctgtcgagc cttcccgtcg tcgcgcctgc cgccagcgcc ggtcaggcgg gcgccggcac
     3481 cccgctatcc actggcaacc tgcgcagcac cggcgagctg tccgcctaca cgcgcaccgg
     3541 gcgctacgac gaggtcgagc gactctgccg ggcctacgca gcgcgctggc cgcggcagct
     3601 gcgctgcgcg gagttcggcc gcacgcccga gggccggccg atgctggcac tcgtcgcctc
     3661 tgccgacgga acgctctcgc cggcgcaggc gcgggcgcgc catcgcccgg tgctgctgtt
     3721 ccagggcggg atccacgccg gcgaaatcga cggcaaggac gcgggcctgc tcgcactgcg
     3781 cgaactgctc gacggaaatg cgcagcccgg cgttctcgga aagatcaccg tcgtgttcgt
     3841 tcctgtgtac aacgtggacg gccacgagcg cttcggccgg tggcaccgcc cgaaccagcg
     3901 cggccccgag gaatcgggct ggcgcaccac ggcgcagaac ctcaacctca atcgcgacta
     3961 caccaaggcc gacgcgccgg agacgcgcgc gatgctcggc ctgctcgacg cctgggaccc
     4021 gatcctgtac gtggacctgc acgtcaccga cggcgcgcag tttcagcccg acatctcggt
     4081 gcagctcgag ccctgcgatg gctgggacca cgagctcggg atcgcgggct gcgccgtgcg
     4141 cgacgccgtg ctcgcggacc tcgagcggca gggttacatc gcgctgccgt tctacccgtc
     4201 gttcgttcgc gacgacgagc ccgagtccgg cttcagcgtg tcggtgtcga acccgaggta
     4261 ctcgacgagc tactgggcga cgcgcaatcg cttttccgcg ctcgtcgaga cgcactcgtg
     4321 gaagccgtat gcgcggcgtg tcgcggccac ccatgcggcg atcctcgcca tggtgggact
     4381 cgccgcgagg gacgggaaga cgtggcttgc cgacgcgagt accgccgacg agcgcgcatc
     4441 gagactggcc gggcagcccg tcgctctctc gtacaagaac tccgatgcga ccaggaccat
     4501 tgaattcccc ggctacgcct actcgcgcga accgtcgccg atctccggcc agtcctggat
     4561 ccgctacgac gaatcctcgc cgcaggtgtg gcgggtgccc ctgctgtacg aggtcgtgcc
     4621 cgatctcgag atcgtcgcgc ccgcgcaggg ctacgtcgtg ccggtcgcct atgccgcgtg
     4681 gatgtcggac cggttgaagc tgcacggcat ccgctacgag gtgctggacc ggccgcagcc
     4741 gcagcttgcg gtgcaggtgt tccgggccga cagcgtcaag caggacgacg agtcgttcga
     4801 agggcacgtc ggcgtgcacg tcacgggcgc gtggagcgac gaggcgcgcg acgtcccggc
     4861 cggctcgctg ttcgtgccga tcgcgcagcc gggggcgcgg ctcgtgatgt cgctgctcga
     4921 gccccaggcg cccgattcct acgtgtcctg gggctacttc gacgcgtcct tcgagcagaa
     4981 ggaatacatg gagaactacg tggcggaggc cgtggcccgg gacatgctgg cccgcgacgc
     5041 gggacttcgc gcggagttcg agcagcgact cgccgccgat ccggccttcg cggccgatcc
     5101 cgacgctcgc ctggatttct tccgccgccg gcatccttcc tgggacgacc ggtacgacct
     5161 ctaccccgtc taccgccgct gacgaaacgc ggcctcgacc cggaatccag gggccgcgcc
     5221 ggccgcgcgc ggatcgcctg gtcagtcttc cgtctggcgg aagtccacgc cgagcgagcg
     5281 gagcttgcgg tacaggtggg tgcgctccat gccaacgcgc ttcgcaagct gcccgacctt
     5341 gccaccacag agctgcagct gctgggtgag gtacgcgcgc tcgaagtgct cacgcgcttc
     5401 gcgcagcggc atcgacagca ggtcctgctt caccagcggc tcgtccgccg cggcctgcgc
     5461 ggcgatctcg ctctcgattt cctcgagccc gatttccgcc ggcccgccgt ggatgagcag
     5521 gcggtgcacg aggttcttga gctcgcggat gttgcccggc caggggtagt tgcgcaggcg
     5581 gttctgcgcg gccacgctga agcggcggaa ccccaggcgc tcgtcttcca ccagccggtc
     5641 gacgtagtgg cgcagcaggt ccggcacgtc ctccgcatac tcacgcagcg gcgggatccg
     5701 cagcatcagc acgctcaagt gcgacagcag gtcgacgcgg aacggctcgg cgccgcggcc
     5761 ctcgaatccc ggctgggccg aggacacgat gcgcgtgtcg aaccgcaccg aagtgctgcc
     5821 cccgacgcgc cggaactccc cggcgtcgat cgccgcgaac aacaggcgct gcgcgaccgg
     5881 ccccaggtca ccgagctcgt tcaagaacag cgtgccgtgc tcggcctgct cgatgagccc
     5941 cttgcgcacc gtgccgcccg actccgagcc gtacagcgcc gcctcggccc cgtcggccgt
     6001 gagcgatccg gcgacggcca tgacgagcgg gccggccgaa cgcgggctga gcgagtgcag
     6061 gtagcgcgcg aacgcctcgc gtcccgtgcc cggctcaccg acgatcagca ccggcgcatc
     6121 gtgcgccgcg agcagcttca cctgctcgcg cagttcgcgc atcacgcggc tgcgacctgc
     6181 cggcgcgatc agcggcggga ccatcgcccg cgtcgcccgc cgcctgcggt cgccttcctc
     6241 gagcgcgctg cgcacggtgc gcagcaactt cgcgagcgac agcggcttct cgacgtagtc
     6301 aatcgcgccg aggcgcgtcg cctcgaccgc cgtctccacc gtcccgtggc cggacatcat
     6361 gacgaccggc aacgcgctgc cgtacgactg ctgccactcc cggagcaggc tgatgccgtc
     6421 ggtccccggc atccagatgt cgagcagcac gaggtccggc tgctgcacgg cgcacgcggc
     6481 gcgcgcctcc tccgccgagg cggagacgct caccgcataa ccctcctcgg tgaggatttc
     6541 ctgcaccagc tcgcggatgt cggcctcgtc gtcaacgacc agtatccgtg cgttactcat
     6601 gcacgctctc tccttggctc ggcccggcgc gcctcgcgcc tggcctggac tcccgatgtg
     6661 tcactgaccg gcagcccgat gcgcacgcgc gccccgccgt ccgggcggtt gtcggcctcg
     6721 atgctgccgc cgtgctcctc gacgatcttg cgcacgatgg cgagcccgag ccccgtcccg
     6781 cgcgccttgg tcgtaacata agggtcgaac acctgcccga ccagctcgcg cgggaagccg
     6841 gggccattgt cctccacgac cacctcggcg atcccggcac cgccgctgtc ggcgaggcgc
     6901 gtcgagaccc ggatgccgcc gtcgggatgg ccctccagcg cctcgaccgc attggtgagc
     6961 aggttgtgca gcaactgccg caggcgtccc tggtcggcga ccacggtgcc gagcgacgga
     7021 tcgagctgca cctccatgcg cactccccgg cccgtcacct gcgcccggta caggtcggtg
     7081 acctccgtca ccagcttgtt caggccgacc ggcgccatct cgaagcgcgg tgcgcgcgcg
     7141 tactcgctga acgcgttcac catctccttc atcgcctcga cctgctgcac gatcgtctcg
     7201 gtgccgcggt cgagcagctg cgcgtccttc tcgctcatgg acggcatcag cttgagccgc
     7261 atgcgctccg ccgagaggcg gatgggcgtg agcgggttct tgatctcgtg cgcgagccgc
     7321 cgcgcgacct cgccccatgc ggcctcgcgc tgcgcctgca gcatcgcggt gatgtcgtcg
     7381 aacaccagca cgtaaccgcc gggcgcttcg ccctcgcccg gcagcggcgt gcaggcgcac
     7441 atgatgatgc ggcgcccggt gcccgccctg aaggtcagct gctcgcgcca ctcgaggtcg
     7501 cccgcgccga ggcgcgcgcg gcacgactcg acgaactgcc gcaacagccc ttcccgctgt
     7561 cccgtctccc cgagctctcc cagccgcaac ccgacgcagg gctcgaggtc ctcgccgagg
     7621 atcatgccgg ccgcgcggtt cacggtgcgc acgcgcaggt ccggctcgag cgacatcacg
     7681 cccgtcgaca ggcgcgcgag gatcacggcg aggttcgcac gctcggtctc ggccgcctgc
     7741 tgcgcgcgct gctggtcctc gcgcgcgcgg cgcaggcgct tggtcatgtc gttgaacgag
     7801 tgcaccagga accccatctc gtcgtgcgac gtgagcggca ggcgcgtgtc gagatcgccc
     7861 gcggccaccg cgcgcgtgcc cttgacgagg tcctggatcg gctgcacgag cttctgcgcc
     7921 gtgaagatcg cgccgtacca tgccgcgagc agcgacagca gcagcacgac ggtcagcgtc
     7981 aggatgaaac tcgccttgag cggtcgccgc aggtaatcct tttcgccgaa ttgcttgtag
     8041 gcggcctgca cggtgtccgc gagcgcaccg agccgctcgg ccaccgggta catcgcctgc
     8101 agcaccaggc ggtcgcgggc gggaccggtg tccagcagcg ggaccgccgt gcgcacgagg
     8161 tacccgccct cgggctccgg ctccaggctc acgaacggcc ggccctgcct gacctgcagc
     8221 aggacctcgc ccggcacctc gtccggcacg acctccgagg acagtccctt gctgaccgcc
     8281 atgagcgtcc cggcctcggt cgagacgacg atgtcgagcg cgccgacctg ctgccgcagc
     8341 gactccagca gcccgggcat cgtgccaccg ctgttcgcga gcgcgtcggc gatcatctgc
     8401 gtgtccgcga ggtgctcgcg catgcgcgcc tcgagcgcgg aacggctcag gaccagcgcg
     8461 tcgtccaggc tcttgcggat ctcgacgttg aaccagctgt cgatgccgcg gttcaggaac
     8521 tgcacggcga agaagtacac cagcaatatc ggcagcgtcg ccagcacgcc gaaggtccac
     8581 accatgcgcg ccttgagtcg cgagccgatg acctgcccgc gccagtcgcg caccagctgc
     8641 acgatcctcg tcgcgagcag cgtcaccagc acggcgagcc cggcgacgtt gatgccgagg
     8701 atccacgggt gcaggcggct gaactggtcc gagttctgcg ccgtctgcgc catgaacagg
     8761 atcgcggcga gccacagcag cccgccgagg cccatcagcg ctgccgacag cgcgcggctc
     8821 agggtcggag cggccattcg taccactcgc tcttcgcgct ccagtcgtcg gtccagaaca
     8881 ggatccacct gagcgcttcg gagccggtat tgaggtcgac cacggcacgc accgcggctt
     8941 catagctggg acccggctgc acgagggtcg cgtcgacgac cggcagcccg cgcacctcgc
     9001 agagtcggtc catcgccgca accagcgtgc cgaagtcgtc ctgctcgccc gtattgaggt
     9061 tgcgcagcag gtagcgctgg ctcagggcgt ggtagctcag ctcgtagctc tgcacgagcg
     9121 aagcgaccgt ggcgtccggc aggtagttgc gcacgcggtt caactcgatc tcgtagtcga
     9181 gccgcagcgt gagcccggcc tcgatggcct tgcgggcgtc gtccggcagc cgcacctcga
     9241 tccgggcgtc gagttcgtac acgtcctggt cgaggctgag cgacgcgctg cgcacgttga
     9301 cctgcgtttg cgcgaccgcc gcggtcgccg cgaccgttgc aagaaagcat gcgagcaaca
     9361 tcacgccacg ccgcagtaaa tcctgcgtgc cgcgacgggg cgggcggtgc ctgttgctca
     9421 tttccaacgt caggggcgtc tggtcaggca agcataatag aagccgtcca tgtcggcttg
     9481 ccccgcgtgc acccggtacc ctggcccggt cccggccggc gccggtggcc acccccggca
     9541 gcgctcgccc gtcccgtcgg cggcatcggc cgtgccggcc aggaatccgg ccaccacgtc
     9601 ctcgccctcc gcacgcagcg ccgagcaggt cgtgtagagc aaggtccccc cgggcttcac
     9661 gagcccccag gccgccagca ggagacttgc ctggcgccgt gccagcgccg ggatgtcgcg
     9721 ggctcgccgc agcaccttga tgtccgggtg ccgccggatc accccggtgg ccgagcacgg
     9781 cgcgtcgagc aggacgcggt cgtacgggcg cccgtcccac caggaccggt ggtcaccgac
     9841 gtctcccgcc agcacctgcg ccgttagccc gagcctgcgc aggttgtcgc ggactcgttc
     9901 gaggcgttgc ggcgaaacgt ccagggccgt gacgtcggcc acaccgttga cccgctcgag
     9961 caggtggcag gtcttgccgc cgggcgccgc gcacgcatcc aggatgcgct cgccgggctg
    10021 cggtgccagc agctcgaccg cgagctgcgc cgcagcgtcc tgcaccgaca cgcggccttc
    10081 gccgaagccg ggcagtgaat gcacgtcgac cggcggcgcg atgcgcagcg cgtccggcgc
    10141 gaacgggtga cggtgcacgt cccagcctcg agcgcgaaga tccgtctcgc atgcggcaac
    10201 gtcggtgcgc atgcggttca cgcgcaacca catcggcgga cgcgcgttgt tggcctcgag
    10261 gatcgcctcg cgcgcgtccg ggcactcgcg cccgagcgcg tcgacgaacc agcgcggatg
    10321 cgccgtccgt accgccggct cggcatccac ggcggccagc acggtctccc gctcgcgctg
    10381 gaatctccgc agcacggcat tgacgaagcc gctggctttc gcgaatccca gcgcccgcgc
    10441 ggcctcgacc gtctcggcga tggccgcgtg ctcggcgact tcgcccgaca tcaactggaa
    10501 caggccgacc tcgagcagcg ccctcagctc cggcttgaga tgcccgggtg gctgatcgca
    10561 gagcgcacgc aggaccgcgg ccaggcggaa gtgccagcgc aaggtgccgt agcagagcga
    10621 gtgcttgagc cccctggcgg ctccggactc gcggctggcc gcgagctgct cgtcgagcga
    10681 gcgaccacgg tcgaccgtct gcgacacaac gcgggctgcg gccgcgcgga tttctgccgc
    10741 agacggttcc gcggccatca gcccagggtc gcgcccgcga gcgcgtgcgc gttcaggaat
    10801 tccgcgacgg gcatggccga gcggcctgca agctgcaacc ggcgcacttc cagcacgccc
    10861 gcgcccgtcg cgacgaggag gcgaccgtgg ctcgcctgaa gcacgcagcc cggcgcagcg
    10921 tccccgccgc cggtgtcgac gagaccagca gccgtgtcgg cagccactcg gaatgagtct
    10981 ccgctcgtga tcgccacggc cgctgcttcc cagatgcgca attgccgtcc catccagcgc
    11041 gtctcggcga ccggccacgg gtcgaaggca cgcacctgcc gctcgatctc gaccgccgga
    11101 cgcgaccagt cgatgagcgc ctcctccttg cggagcttcg gcgcataagt tgcgccctcg
    11161 gcgggctgcg gtacggcccc gagccgcccg tcggcccagg cgccgaccgc ggcgacgatc
    11221 gcttcggcgc cgagcaccgc cagccggtcg tgcaggctgc cggcgctttc cccgggcgcg
    11281 atcgtgattt cgcgagagag gagcatcggc ccggtatcca gcccggcgtc catcttcatg
    11341 atggtgatgc cggtccgcgc gtcgccggcg aggatcgcgc gctgtatcgg cgccgcgccg
    11401 cgccagcgcg ggagcaggga cgcatggatg ttgaggcagc cgcggcgcgg tacgtccagc
    11461 acggcttggg gcaggatgag cccataggcc gcgacgacca tgacgtccgg catgaacgat
    11521 gcgagcctcg cgaccgcgtc gtccgtccgt agcgtggcgg gctgctccac cgtcaggccg
    11581 agttcgagcg cccgtcgttt gaccggcccg gcggtgacct cgcgaccgcg ccccgagggt
    11641 cgatcgggct gggtatagac cgccgcaatc ccgtgccccg cgccgtgcag cgcgtcgagg
    11701 gccggcacgg agaagtccgg agtcccggcg aatacgatgc gcagccggtg gttcattgca
    11761 gggacgttgg tgcggcgagc cggcgaccgg ccggcgttcc tagatcacgg gcgcagtcgc
    11821 gcgctcgcgg cgtccagcgg aaccttgctc gcgccgctcc ttctcgagct tcttgcggat
    11881 acgcgtgcgc ttcaggtcgg agaggtaatc gacgaagagc ttcccttcga ggtgatccat
    11941 ctcgtgctgg atgcagattg cgagcagccc gtcggcttcc atctcgaacg gcttgccgtc
    12001 acggccgagg gcgcggacga cgatgcgcgc ggagcgcgtg ttcagcgtgt cgaagatgcc
    12061 ggggaccgac aggcagccct cctccgcttc gaccgtgccc tcgcgctcga tgatctccgg
    12121 attcacgaac gcgagcgggt ggtcctgctc cttggtcacg tcggcgacga ggacccgctt
    12181 gtgcacgtcc acctgcgtgg cggcgagccc gacgcccggc gccgcataca tggtctcgag
    12241 catgtcgtcg atgagccggc gtatgccgtc gtccacgacg gccacgggtt gcgcccgggt
    12301 tcgcagtctc ggatcgggaa attcgaggat cgtaagcttc gccattctct tgaaaatgct
    12361 gctaaagtta agtcgggctt cgatttaaca actgtgagga ccgccctgga cccgtgtcgc
    12421 cgtccagcgg cgtaacgcga ctgagacgtg gtcggcagcc tcccggaggc gtgccggatt
    12481 ataaggcgac ggtcgcgacc ccgacactcg atggagaaag ggatggatcg aaagctgcgc
    12541 gtcgccccga gaatctcgac caggctgagc cactggcccc gggccttggg catggcgctc
    12601 gccgttgcgg gtgtcggcct ggccctgggc gtcaccctcc ccgtcccggc cgcagcgcag
    12661 gaggcgtccg gccatgccat gtccgtgccg ctggcgccgg acgccccgga cacttatgtc
    12721 gtcaagaagg gcgacacgct gtgggacatc gcgggggtgt tcctcaagta cccctggtac
    12781 tggcccgaga tctggtacgt gaacccgcag gtcgccaatc cgcactggat ctacccggga
    12841 gacgtcctgc acctcatcta cgtggacggc aagccgcgcg tcaccgtcgg gaccgcgggc
    12901 gaagtccgcc tgtcgccgca agtgcgcacc gacccgctcg ggcaggcgat ccgcacggtc
    12961 ccgtacgacg tgctgatgaa cttcgtgcag catccgagcc tgctcaccga gaagcaggtc
    13021 gacaaggcgc cgtacatcgt cggcttccgc ggcgggcacc tcgttggtgc tggcgtgaac
    13081 gaggcgtatg ccaccgggct cgacaacccg ccggtgggca atgagtacac ggtcgtccac
    13141 gtcggcacgg agctgcgcga cccggacgac ggcgacctgc tcggctacat cggccattac
    13201 gcggcgacga cccgcgtgat cgccaccacc gacgcgaagc acgaggaccc gatcacccac
    13261 cttgccgtgc tggacaacgg ccgggagatc cagcagggcg acaaggtgct gcccgcgatc
    13321 aacaagttcg gcgacgactt cgtgctgagc gctccgtcgg accgcaagct gaatggccag
    13381 gtgctggcgg tcgtcaacgg actcgcggtc gggggcacct accaggtcct cgcgatcaac
    13441 cgcggcaagc gtgacgggct cgccccgggg aacatcgtcg cgatcttcgc gcgcggactg
    13501 gacgtggcga attactacag ccgcggacag gactggacgt cgttcaccgc gaactactcg
    13561 aaggtgaagc tgccggatga gcgcagcgca acgatgctgc tgttcaccgt gtacgaccgc
    13621 atgagctacg gactggtggt ggaatcgacc cagcccgtgc acatcggcga cttcatcaag
    13681 caccccgact tcgggcacga cgacctggga atgccggcca ccgcccgctg agcgggtcgc
    13741 gcatcggcgc gaacgcgcgc atttcagaaa gagcctgccg ggcggaatcc cggtaggctc
    13801 ttctgtcatg gacgacccga aaccgtggct cacgctcgca cgggcgccgg gcctgcatgc
    13861 cgggcatctg cgcgacccgg agggggcatc catcccgccg ttggccctgc tgcgagagcc
    13921 cgctcgctcg ctcggctcgc tcggactcgg cgccgccatg gtcgaggcga tccgcaatcc
    13981 gtcgaccgcg gggctcgaag cggacgagcg ctggctcgca gggtccggcc gcacgctcgt
    14041 gacctggggc tcgccggagt accctgcgct tctcgcgacg attccggatg cgccgctggt
    14101 gctgttcgtc gcaggagcgg cggcgagtct gcatctaccg cagctggccg tcgtgggcag
    14161 ccgcaacccg acggcgatcg gtcgcgacac cgccgaggat ttcgcgcgcc acctggcggc
    14221 cgcgggattc gcgattacga gcggcctcgc gctgggcatc gacgctgccg cacatcgtgg
    14281 cgcgctcgcg ggccgcggac tcacgatcgc ggtgctcggc tgcgggctcg atcgcgtgta
    14341 tccgcgcgag aatgccgcgc tcgcggagca gatcctggcg cacggcgcgc tcgtctccga
    14401 tctcccggcc ggcacgccgc cgctcaagca gcacttcccg cggcgcaatc gcatcatcag
    14461 cgggcttgca gtgggcacgc tcgtggtcga ggccgcgctg cagagcgggt cgctcatcac
    14521 ggcgcggctc gcggccgagc agggacgcga agtgttcgcg atcccgggct cgatccacag
    14581 tccgctctcg cggggctgtc accgcctcat ccgccagggc gc
//
LOCUS       DAOMFD010000038        14291 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_977_length_14291_cov_33.461225, whole genome shotgun sequence.
ACCESSION   DAOMFD010000038 DAOMFD010000000
VERSION     DAOMFD010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14291)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14291)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14291
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_977_length_14291_cov_33.461225"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(138..1136)
                     /locus_tag="PL152_03820"
     CDS             complement(138..1136)
                     /locus_tag="PL152_03820"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR48436.1"
                     /translation="MSTPLLEVRGLRVEYPARGWGGRRGSVVPALHGLDLALDDGRSL
                     GIVGESGCGKSTLVRSALGLVAPSDGEVRWRGWTVDTRDRARMRQMRREMQVVFQDPF
                     GSLDPRLTAGESVAEALQALEGVTDAAPTRQRVSAAFAAVGLDASLASRYPHELSGGQ
                     CQRVAIARATIVRPRLLICDEPVSALDVSVQAQIVNLLAELRRRDRTALLFISHNLAV
                     VRHLCEEVAVLYLGRIVERAPRDSLFLAPRHPYTRALLAAVPDPDPGEAARRSRAESP
                     TVVADRLPAAGGCPFSPRCPLAVDRCRIEPPELESTGDGAVAACHRWRELPTAQLD"
     gene            complement(1133..2110)
                     /locus_tag="PL152_03825"
     CDS             complement(1133..2110)
                     /locus_tag="PL152_03825"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR48437.1"
                     /translation="MAQAPLLAIDGLRVAFDAEGGTLQAVRGVALDVRPGECLAVVGE
                     SGSGKSQLFLACLGLLAENGRATGSACLDGDELIGSPEAQLETVRGARVGLVSQDPMN
                     ALTPHLRIGAQLVEYVTDRGLMSRSQARARALELLGAVGIDDPESRFGQYPHELSGGQ
                     RQRVAIAMALMPGPGLLVADEPTTALDVTVQAQVLQVLRRLREGGLTIVLITHDLAVV
                     AGVADRVAVMYAGVVVELAPVAGLFASPRHPYTRALLDSIPRLDAPADRLSGIEGQPP
                     GPHERIVGCAFAPRCRQARDQCRRSEPAPQAAGESVWACHAPLPRGWTT"
     gene            complement(2112..2978)
                     /locus_tag="PL152_03830"
     CDS             complement(2112..2978)
                     /locus_tag="PL152_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR48438.1"
                     /translation="MSDGPRRGIYVAAWRRLRGNRAAVVALATLVLLVAASLAGPAVS
                     PYAIDQVDWSLNPLASPPALGTGHWFGTDGNGRDLFVRVCAGTRISLLIAVLATAVSL
                     AIGVAWGATAGYVGGRLDDWMMRVVDVLYALPYMFFVIILTVVFGRNLFLIFLAIGAV
                     GWLTMARIVRGQALALRSQPFVDAAITLGLPTHRIIARHIVPNVVGTVVVYATLTVPQ
                     VILFESFLSFLGLGVQEPLTSLGRLVAEGSLEMESAPWLLLAPSTVLALILLCLNFLG
                     DGLRDALDPRQR"
     gene            complement(2971..3897)
                     /gene="oppB"
                     /locus_tag="PL152_03835"
     CDS             complement(2971..3897)
                     /gene="oppB"
                     /locus_tag="PL152_03835"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HQR48439.1"
                     /translation="MYLRYAVRRLLGIVPTLFVIVTAAFFLMRLAPGGPFDAEHMPPP
                     EIRANLQAAYGLDQPVLVQYGRYLAGLLHGDLGPSFRLKDYGVAELIGDGLPVTLSIG
                     GAALLLAVVLGVPLGVLAGHRHNGWVDHGVMATALAGIAVPNFVVAPLLALVFGITLK
                     WLPVAGWEPGSPRYMILPVVTLALPFIAYVARLTRGSLLEVLQSPWIRTARAKGITGA
                     AILRRHALKPALMPVLSFLGPAAAALLTGSLVVEQVFGLPGVGRYFVQGAVNRDYTLV
                     MGMVVFYATLILLLNLAVDLAYGWLDPRIRHE"
     gene            complement(3897..5918)
                     /gene="ligA"
                     /locus_tag="PL152_03840"
     CDS             complement(3897..5918)
                     /gene="ligA"
                     /locus_tag="PL152_03840"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HQR48440.1"
                     /translation="MAVSDVARRISELRAQIEHHDYRYYVLDDPEISDARYDALMREL
                     REIEAKHPELATPDSPTQRVGGAPSHGFAEVRHSLPMLSLDNAFSKEDVTEFDRRVRE
                     RLDVASVDYSAEPKLDGLAISVRYEQGLFVQAATRGDGTKGEDVTANVRTIGSLPLRL
                     RKGAPKLLEARGEVFMTRRSFERLNQRAAESGEKTFANPRNAAAGSLRQLDPRVTADR
                     ALDVCFYGLGTTEGWASPRRHGEVLAGLREFGLRTSPDAEVVAGVEGCLAYFERMGKR
                     RDRLEYDIDGVVYKVDRLDWQKDLGFVARAPRWAIAHKFPAQEETTTVRGVDFQVGRT
                     GALTPVARLDPVFVGGVTVSNVTLHNMDDLERKDVRIGDTVVVRRAGDVIPEVVRVVV
                     DRRPAGARRVRLPSQCPVCGSHVTRAEGEAVARCSGGLVCPAQRREALLHFASRRAMD
                     IEGLGDKLVEQLVESGRVKTPADLYRLTAGELAALERMGEKSAANLVEALERSKHTAL
                     ARFLFALGIRDVGEATALALGEHFGSLDALQDASIDEIQQVRDVGPVVARHVRDFFDE
                     AHNRKVIRELRGLGVAWPEGEGIGRASGGPLQGQTVVITGALGSMTRDDARAAARAAG
                     ATVTDSVSRKTTLLVTGEDPGSKLRKARDLGVRIVDEAEFLRLLGRGAA"
     gene            complement(5926..6762)
                     /locus_tag="PL152_03845"
     CDS             complement(5926..6762)
                     /locus_tag="PL152_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA C-terminal
                     FtsZ-binding domain-containing protein"
                     /protein_id="HQR48441.1"
                     /translation="MGALRWILLAAGLALIAGLYLWGTRGKQRSGGTERGRTLPVDSP
                     SASTAMVAEPAAPVRVEPQVRLDEQTPPIHEVVPLVEIGDEAGTAASRREPTLGQRLV
                     APQPRRVAEEPPPETPGPPPAEEDVESQTAVEARAQRIVALRVTAPLPSRFEGSLLFE
                     ALAAEQFTFGRYQIFHKVDATGRPVLSLASLREPGTFDPATMAGAAFAGVALFAVLPG
                     PLTGQQAFEELLGAARSLATRLGGHLQDDRGAPLTAQRIMRLREEMLAFDRGRAAEPV
                     RR"
     gene            complement(6772..10278)
                     /gene="smc"
                     /locus_tag="PL152_03850"
     CDS             complement(6772..10278)
                     /gene="smc"
                     /locus_tag="PL152_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HQR48442.1"
                     /translation="MRLTKIKLAGFKSFVDPTTISFPSSLVGVVGPNGCGKSNIIDAV
                     RWVMGEISAKHLRGDSMADVIFNGSGSRKPVGHASVELVFDNADGKLGGEYANYAEVS
                     LRRVVSRDGTSSYFLNGTRCRRKDITQLFLGTGLGSRSYAIIEQNMISRVIEAKTDEM
                     RAFIEEAAGISKYKERRRETESRIGHTRENLARLSDLRDEVEKQLRHLQRQAATARRY
                     QALKQEERQLHAELLALRLREVESEAGARQAIMSERELAMQAALAGQRSIEAAIERAR
                     RDHDAQGEVLREVQGRYYQVGADISRTEQAIEHARELRSRQRQELGQVAQGLEEAGAH
                     RARDAAQAAELALLLAEIEPGLVAARSAEESSANRLAESEAAMHAWQERWEAFNQSLR
                     ESSSAAEAERARLGEMDVHVKRLAEQRERASQEHAVLSAADVAAKINELDRAESAARG
                     ALEAARNRIAGLVADLQAERARERELAEAAQGARRDAQEAEGRVVSLEALQKAALGQG
                     QGKVVEWLESRGLNGNPRLAQQLAVDSGWERAVETVLGSYLEAVCVDSIDDVAGTLAS
                     LSSGTVTFFAGGQGAPLPGGAGGALRSRVRGPAGLDALFEGIASVDTLAEALAARPSL
                     RAGQSVITRDGIWIGPDWLRVSRDRDVHEGVIEREQALRRERDVLAGLGSRRDELERQ
                     LDAHRAGIRAKEDELAADQARLAQTGAEHMAARSQLDSLRARSEQREARMRQLAGELA
                     ELDSALEVATAGSRSARGRLEEAVDAMAALDERRRLLESERDALRQALAEARRLAEEH
                     RNAAREVAIRVESRRTSHESLLAGIARLDGQISQFAARRADLEAQLAAGEAGEADLKL
                     ALDTALSRRVVVDEELAVARRTMDEVDAKLRELDEQRVVAEAAVQEARDLLENVRFSV
                     QELRVRREALLEQFGATRLEFSEVVAALAEDANVTGWEQRLAEAADKIERLGQVNLAA
                     IDEFREQSERKEYLDRQFADLNDALATLEDAMRKIDRETRTRFQDTFDRVNAGLKDKF
                     PRLFGGGHAYLELVGEDVLESGVAIMARPPGKRNSSISQLSGGEKALTAVALVFSIFE
                     LNPAPFCLLDEVDAPLDENNVGRFCEIVRDMSSRVQFVFVTHNKATMELASQLLGVTM
                     NEPGVSRLVAVDVDEAVRLAAI"
     gene            10844..11224
                     /gene="dksA"
                     /locus_tag="PL152_03855"
     CDS             10844..11224
                     /gene="dksA"
                     /locus_tag="PL152_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017524830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HQR48443.1"
                     /translation="MSKEQLEHFRAILSDWKQDLMQEVDRTMLHMKDEAANFPDPNDR
                     ATQESEFSLELRTRDRERKLIRKIDEALKRIEDGSYGFCLETGEPIGVKRLEARPVAT
                     LSVEAQERRERREKQFGDRDDWYR"
     gene            11175..12146
                     /gene="gluQRS"
                     /locus_tag="PL152_03860"
     CDS             11175..12146
                     /gene="gluQRS"
                     /locus_tag="PL152_03860"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF004314.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HQR48444.1"
                     /translation="MSGAKSSSATATTGTVEGATPCRGRFAPTPSGALHFGSLVAALA
                     SWLDARARGGQWLVRIEDLDPPRERPGAADDILATLESLGLHWDGPVLRQSSRHDAYD
                     AALERLRRYGRLRECACPRTALASLPQNAQRASGDDLYHPPECVVGAGHRGGIALRFR
                     VPDGEVEFLDRAQGAVRVNVARSVGDFVLRRRDGLYAYQLAVVVDDEAQGITDVVRGA
                     DLLSSTPRQVLLQDALGLRRPKYMHVPLAVDAGGLKLSKTSDAPRLAGAAPAAQLAAA
                     LDFLGQEPPPDAAGAAVGDVLRWAAAHWDPARFAGSTSRATPALARD"
     gene            12245..12709
                     /gene="phaR"
                     /locus_tag="PL152_03865"
     CDS             12245..12709
                     /gene="phaR"
                     /locus_tag="PL152_03865"
                     /inference="COORDINATES: protein motif:HMM:TIGR01848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis repressor PhaR"
                     /protein_id="HQR48445.1"
                     /translation="MIRKYPNRRLYDTVESRYVTLADIRRLVVERIDFVVLDRKNQQD
                     ITRSILLQVIAEQEGAGESLMSRDFLSHVIRTYGSGLQDFVGRYLDESIRQFAREQRD
                     LRDKLKSVVGVDPVETVTAVAQKNYQRWKELQEEVFSRLPGSWGREKEKDKE"
     gene            complement(12822..>14291)
                     /gene="pnp"
                     /locus_tag="PL152_03870"
     CDS             complement(12822..>14291)
                     /gene="pnp"
                     /locus_tag="PL152_03870"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017391359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQR48446.1"
                     /translation="FGHEQMQVAIKAIRELAAEAGKPAWAWSAPAVDPALVAAVASQA
                     EAALGEAYTITDKQKRYARIGEIKKATVEALAGGESPRFDARKVSDQLGMLEYNLVRG
                     RILAGQPRIDGRDMHTVRKITVQTGVLPRTHGSALFTRGETQALVTTTLGTGRDAQLI
                     DALAGERKEPFMLHYNFPPFSVGETGMMGSPKRREIGHGNLARRGVSAVMPDMATFPY
                     VIRVVSEILESNGSSSMASVCGTSLSLMDAGVPLKAPVAGVAMGLVLEGGKFQVLTDI
                     LGDEDHLGDMDFKVAGTSNGVTALQMDIKIEGITREIMKVALEHAHKARLHILGEMAK
                     VITEARPQMSEWAPTIITIKIDPEKIREVIGKGGAVIRAITEETGTTIDIENDGTVKI
                     ASVNGAAGREAQRRIELITSEVEVGAIYEGKVVRLMDFGAFVQILPGKDGLVHISQIS
                     DERVERVSDKLREGDVVRVKVLEVDRQGRVRLSMRNVDA"
BASE COUNT         2133 a         5205 c         4890 g         2063 t
ORIGIN      
        1 agcgtgtgca cgaagccgat ggaagcggcg gtgagcagca gcagtccgag atcgatgccc
       61 atagtcgcgc gccacctcca ccggcaggtg gcggaaggta aacacaggcc atttccgccg
      121 gcaaggcggg gaaagaccta gtccagctgc gccgtgggca gctcccgcca gcggtggcag
      181 gccgccacgg ccccgtcgcc ggtggactcc agctcagggg gctcgattcg gcagcgatcg
      241 acggccaggg ggcagcgagg cgaaaacggg cagccgccgg cggccggcag ccggtccgcg
      301 accaccgtcg gcgattcggc ccgcgaccgc cgagccgcct cgccgggatc gggatccggc
      361 acggcagcga gcagcgcgcg cgtgtacggg tggcgcggcg ccaggaacag cgaatcgcgc
      421 ggcgcacgct cgacgatgcg cccgaggtac agcacggcga cttcctcgca aaggtgccgc
      481 accacggcga ggttgtggct gatgaacagc agcgcggtgc ggtcgcgccg gcgcagctcg
      541 gcgagcaggt tgacgatctg cgcctgcacg gagacatcga gggcgctgac cggttcgtcg
      601 cagatcagca ggcgcggcct cacgatcgtg gcccgcgcga tggcgacacg ctggcactgt
      661 ccaccactca gttcgtgcgg gtagcgcgac gccagcgagg catcgagccc gaccgccgcg
      721 aacgccgccg acacgcgctg ccgggttggc gccgcatccg tgacgccctc gagggcttgc
      781 agggcttctg caacgctctc cccggcagtc aggcgcgggt ccaggctgcc gaaagggtcc
      841 tggaacacga cctgcatctc tcgccgcatc tgtcgcatcc gtgcacggtc cctcgtgtcc
      901 acggtccagc cgcgccagcg cacctcgccg tcgctcggcg cgacgagacc cagcgcgctg
      961 cgcacgagcg tcgacttgcc gcagcccgat tccccgacga tgccgagcga tcgtccgtca
     1021 tcgagggcga gatcgagccc gtgcagtgcc ggcacgacgc tgccccgccg gccgccccat
     1081 ccgcgcgccg ggtactccac gcgcagcccg cggacttcca gaagcggcgt gctcatgtcg
     1141 tccagccgcg cggcaagggc gcatggcacg cccagactga ttcgccggcg gcctgcggag
     1201 cgggctcgga tcggcggcac tggtcgcgcg cctgtcggca acgcggcgcg aatgcgcagc
     1261 ccacgatgcg ttcgtgaggg ccgggcggct ggccctcgat gcccgacagt cgatccgcag
     1321 gcgcatcgag tcgcgggatc gagtcgagca gcgcacgcgt gtacgggtgc ctgggcgatg
     1381 cgaagagccc ggcgaccggg gccagttcca cgacgacgcc tgcatacatc accgcgacgc
     1441 gatcggcgac gccggcgacc acggcgaggt cgtgcgtgat gagcacgatc gtgagtccgc
     1501 cctcgcggag gcgacgcaat acctgcagga cctgcgcctg caccgtcacg tcgagcgcgg
     1561 tcgtcggctc gtcggccacc agcagcccgg gcccgggcat caaggccatg gcgatcgcca
     1621 cgcgctgccg ctggccaccg gagagctcgt gagggtactg gccgaacctc gattccggat
     1681 cgtcgatgcc cacggccccc agcagttcca gcgcccgtgc tcgcgcctgc gacctgctca
     1741 tgagcccgcg gtcggtcacg tactcgacga gctgcgcgcc gatgcgcagg tgcggcgtca
     1801 gcgcgttcat ggggtcctgc gacaccagtc cgacgcgcgc accgcgcacc gtctcgagct
     1861 gcgcctcggg gctcccgatg agttcgtccc cgtcgaggca cgcggagccc gtggcgcgcc
     1921 cgttttcggc cagcaggccg aggcacgcga ggaagagctg gctcttcccc gagccggact
     1981 cgcccaccac cgccaggcac tcgccgggcc gtacgtccag cgcgacgcca cgcacggcct
     2041 gcaatgtccc gccctcggcg tcgaaggcga cgcgcaggcc gtcgatcgcg agcagcggcg
     2101 cctgggccat gtcagcgttg ccgcgggtcg agcgcatcgc gcagcccgtc gccgaggaag
     2161 ttcaggcaca gcaggatcag cgcgagcacc gtcgacggcg ccagcagcag ccacggcgcg
     2221 gactccatct cgagcgagcc ctccgccacg agccggccca ggctcgtgag cggctcctgc
     2281 acgccgagcc cgaggaagct caggaagctc tcgaacagga tgacctgcgg caccgtgagc
     2341 gtcgcgtaga ccacgaccgt gccgacgacg ttcggcacga tgtgtcgcgc gatgatccgg
     2401 tgcgtgggca ggccgagcgt gatcgccgca tccacgaacg gctggctgcg cagggccagc
     2461 gcctggccgc gcacgatgcg cgccatggtg agccagccga ccgcgccgat cgcgaggaag
     2521 atcaggaaca ggttgcggcc gaatacgacc gtcaggatga tgacgaagaa catgtacggc
     2581 agcgcgtaca gcacgtccac cacgcgcatc atccagtcgt ccaggcgccc gccgacgtag
     2641 cccgcggtcg ccccccaggc gacgccgatc gcgagactca ccgccgtggc cagcaccgcg
     2701 atgagcagcg atatccgggt tccggcgcag acgcggacga acaggtcgcg gccgttgcca
     2761 tcggtgccga accagtgtcc cgtcccgagc gccggcggcg acgccagggg gttgagcgac
     2821 cagtcgacct ggtcgatcgc gtacggactc acggccgggc cggccaggga tgcggcgacc
     2881 agcaggacca gtgtcgccag cgcgacgacg gcggcacgat tgccgcggag gcgccgccat
     2941 gcggcgacgt agattccgcg gcgtggcccg tcactcatgg cggatgcggg ggtcgagcca
     3001 gccgtacgcc aggtccacgg ccaggttgag cagcaggatc agcgtcgcat agaaaacgac
     3061 catgcccatg acgagcgtgt agtcgcggtt cacggcgccc tgcacgaaat accgcccgac
     3121 ccccggcaag ccgaagacct gctccaccac cagcgagccc gtcagcagtg cggcggcggc
     3181 cgggccgagg aagctcagca ccggcatgag cgccggcttc aacgcgtggc gacgcaggat
     3241 ggcggctccg gtgatgcctt tcgcccgtgc cgtgcggatc cagggcgact gcagcacctc
     3301 gagcaggctg cctcgcgtca ggcgcgcgac gtaggcgatg aacggcagcg ccagggtcac
     3361 caccggcagg atcatgtaac gcggactgcc cggctcccaa ccggcgaccg gcagccactt
     3421 gagcgtgatg ccgaacacga gcgcgagcaa cggcgcgacg acgaagttcg gcacggcgat
     3481 gccggcgagc gcagtcgcca tgacgccgtg gtcgacccag ccgttgtgcc tgtggcccgc
     3541 gagcacgccg agcggcacgc cgagcacgac ggcgagcagc aacgcggcgc cgccgatgga
     3601 cagcgtgacc ggcaggccgt cgccgatgag ttccgccacg ccgtagtcct tgaggcgaaa
     3661 cgacgggccg aggtctccgt gcagcagccc ggccaggtag cgtccgtact gcacgaggac
     3721 cggctgatcg aggccgtagg ccgcctgcag gttggcccgg atctcgggcg gcggcatgtg
     3781 ctccgcgtcg aatggcccgc ccggggccag gcgcatcagg aagaacgccg ccgtgacgat
     3841 cacgaacagc gtcggcacga tcccgagcag gcggcggacg gcgtagcgga ggtacattac
     3901 gccgctcccc ggccgagcag gcgcaggaat tcggcctcgt ccacgatgcg gacgcccagg
     3961 tcgcgtgcct tgcgcaactt ggatcccgga tcctcgccgg tcacgagcag cgtggtcttg
     4021 cgggacacgg agtcggtcac cgtcgcgccc gcggcacggg cggcagcgcg tgcgtcgtcg
     4081 cgcgtcatgg acccgagggc gcccgtaatc acgacggtct ggccttgcag cgggccaccg
     4141 gacgcgcggc cgatgccctc gccttccggc cacgccacgc caaggccgcg caactcccgg
     4201 atcaccttgc ggttgtgtgc ctcgtcgaag aaatcccgga cgtgcctggc cacgaccggc
     4261 cctacgtcgc ggacctgctg gatctcgtcg atgctcgcgt cctgcagggc atcgaggctg
     4321 ccgaaatgtt cccccagcgc gagtgcggtc gcctcgccca cgtcgcggat gccgagtgcg
     4381 aagaggaatc gcgccagggc ggtgtgcttg ctgcgctcca gggcctcgac caggttcgcg
     4441 gccgacttct cgcccatccg ctcgagcgcc gccagttccc cggccgtcag cctgtacagg
     4501 tcggccggcg tcttcacgcg acccgattcc accagttgct ccaccagctt gtcgcccagc
     4561 ccttcgatgt ccatggcgcg tcgcgaagcg aagtgcagca gggcttcacg ccgctgcgcc
     4621 ggacagacga gcccgccgct gcaacgcgcg accgcctcgc cctccgcgcg cgtcacgtgg
     4681 gagccgcaca ccgggcactg gctcggcaac ctgacccgtc gcgccccagc gggccgccgg
     4741 tcgaccacga cgcgtacgac ttcgggaatc acgtcgccgg cgcgccgcac cacgaccgtg
     4801 tcgccgatgc ggacgtcctt gcgctcgagg tcgtccatgt tgtgcagggt gacgttgctc
     4861 accgtcaccc cgccgacgaa caccgggtcg agccgcgcga cgggcgtgag cgcaccggtg
     4921 cggccgacct ggaagtcgac gccgcgcacg gtcgtcgtct cctcctgcgc cgggaacttg
     4981 tgcgcgatgg cccagcgcgg ggcccgcgca acgaagccga ggtccttctg ccagtcgagg
     5041 cggtccacct tgtagaccac gccgtcgatg tcgtactcga gccggtcgcg acgcttgccc
     5101 atccgctcga agtacgcgag gcacccctcg acgccggcga ccacctcggc atccggcgag
     5161 gtgcggagcc cgaactcgcg caatcccgcc agcacctcgc cgtggcgccg cggcgaagcc
     5221 catccctcgg tcgtcccgag cccgtagaag cagacgtcta gcgcacggtc cgccgtgacg
     5281 cgcgggtcga gctggcgcag gctgccggcg gcagcattgc gcgggttggc gaaggtcttc
     5341 tccccggact ccgcggcgcg ctggttgagt cgctcgaaag agcggcgcgt catgaacact
     5401 tcgccgcggg cctcgagcaa cttcggcgct cccttgcgca ggcgcagcgg cagcgacccg
     5461 atggtgcgca cgttggcggt cacgtcctcg cctttcgtgc cgtcaccgcg cgtggccgcc
     5521 tgcacgaaca ggccctgctc gtagcgcacg ctgatcgcga gcccgtcgag cttcggctcc
     5581 gccgagtagt cgacgctcgc cacgtccagc cgctcgcgca cgcggcggtc gaactcggtg
     5641 acgtcttcct tgctgaaggc gttgtcgagc gacagcatcg gcagggagtg ccgcacctcc
     5701 gcgaatccgt gcgacggcgc gcccccgacg cgttgcgtgg gcgagtccgg cgtcgccagt
     5761 tccgggtgct tcgcctcgat ctcccgcaat tcgcgcatca gcgcgtcgta gcgggcgtcg
     5821 gagatctccg ggtcgtcgag gacgtagtac cgatagtcgt ggtgctcgat ctgcgcgcgc
     5881 agttccgaga tgcgccgggc gacgtcactg acagccatcg cgccgtcagc gccggacggg
     5941 ctcggcggcc cggccgcggt cgaacgcgag catctcttcg cgcagccgca tgatgcgctg
     6001 cgccgtgagc ggcgcgcccc ggtcgtcctg caggtgtcca ccgagccgcg tcgccagcga
     6061 tcgcgcggcg ccgagcagct cctcgaacgc ctgctgcccg gtcaggggcc ccggcaggac
     6121 cgcaaacagc gccaccccgg cgaaggccgc gcccgccatc gtcgcgggat cgaacgtacc
     6181 cggttcgcgc aggctcgcga ggctcagcac gggacgtccc gtcgcatcga ccttgtggaa
     6241 gatctggtag cgaccgaagg tgaactgctc ggccgccaag gcctcgaaca gcagcgagcc
     6301 ctcgaaccgc gacggcagcg gggccgtcac gcgcaacgcc acgatgcgct gggcgcgcgc
     6361 ctccacggca gtctgcgatt cgacgtcctc ttccgccggc ggcggcccgg gagtctccgg
     6421 aggcggctcc tcggcgacgc gtctcggctg cggcgcgacc aggcgctggc ccagcgtcgg
     6481 ttcacgccgg ctggcagcag tccccgcctc gtcgccgatc tcgaccagtg gaacgacctc
     6541 gtggattgga ggtgtctgct cgtcgagtcg aacctgcggc tcgacccgga ccggggctgc
     6601 gggctccgca accatggcgg tggaagccga cggcgagtcc accggcagcg tccggccgcg
     6661 ctcggttccc ccgctccgtt gcttgccacg ggttccccac aggtacagcc cggcgatcag
     6721 tgccaggccg gccgcgagca gtatccagcg cagggccccc atcgtgtccc gtcagatcgc
     6781 cgcgagcctc acggcctcgt cgacgtccac cgcgacgagc cgcgacacgc ccggctcgtt
     6841 catggtcacg ccgagcagct ggctcgcgag ttccatggtc gccttgttgt gcgtgacgaa
     6901 tacgaactgc acccggctcg acatgtcgcg cacgatctcg cagaagcggc cgacattgtt
     6961 ctcgtcgagc ggcgcgtcga cttcgtcgag caggcagaac ggcgccgggt tgagctcgaa
     7021 gatcgaaaag accagcgcca ccgcggtgag cgccttctcg ccgcccgaca gctggctgat
     7081 cgagctgttc cgcttgcccg gcgggcgcgc catgatcgcg actcccgact ccaggacgtc
     7141 ctcgccgacc agctccaggt aggcgtgacc gccgccgaac aggcgcggga acttgtcctt
     7201 gagcccggcg ttgacgcggt cgaacgtgtc ctggaagcgc gtgcgggtct cgcggtcgat
     7261 cttgcgcatc gcgtcctcca gcgtcgcgag cgcgtcgttc aggtccgcga actgccggtc
     7321 caggtattcc ttgcgctcgg actgctcgcg gaactcgtcg atcgctgcca ggttcacctg
     7381 cccgagccgc tcgatcttgt cggccgcctc cgccaggcgc tgttcccatc cggtcacgtt
     7441 ggcatcctcg gccagcgccg cgaccacttc ggagaattcg agcctcgtcg cgccgaactg
     7501 ttcgagcaac gcttcgcgcc ggacgcgcaa ctcctgcacc gagaacctga cgttctcgag
     7561 caggtcgcgc gcctcctgca cggccgcctc ggccacgacg cgctgctcgt cgagctcgcg
     7621 cagcttcgcg tccacctcgt ccatggtccg gcgtgccacc gcgagttcct cgtcgaccac
     7681 cacgcggcgc gaaagcgccg tgtccagggc cagcttgagg tccgcttcgc cagcttcgcc
     7741 ggcggcgagc tgcgcctcca ggtcggccct gcgcgccgcg aactggctga tctgcccgtc
     7801 gagtcgggcg atgcccgcca gcagggactc atgactcgtg cgtcgcgatt cgacgcgtat
     7861 tgcgacctcc cgcgcggcgt tgcggtgctc ttccgcgagc cgccgcgcct cggcgagcgc
     7921 ctgccgcagc gcgtcgcgct cgctctcgag gagccgccgc cgctcgtcga gcgcggccat
     7981 cgcgtcgacc gcctcctcga gacgaccacg cgccgagcgg ctgcccgcgg tcgccacttc
     8041 cagcgcgctg tcgagttccg cgagctctcc ggcgagctgc cgcatgcggg cttcgcgctg
     8101 ctccgagcgg gcccgcaggc tgtccagttg cgatcgcgcg gccatgtgct ccgcaccggt
     8161 ctgcgcgagg cgcgcctggt cggcggcgag ctcgtcttcc ttggcgcgga tgccagcccg
     8221 gtgcgcatcg agctgccgct cgagttcgtc gcgcctcgag cccagccctg caagcacgtc
     8281 acgttcccgc cgcagtgctt gctcgcgctc gatgacgcct tcgtggacgt cccggtcacg
     8341 gctcacgcgc agccagtccg gcccgatcca gatgccgtct cgcgtaatga ccgactggcc
     8401 ggcgcgcagg ctcggtcgcg ccgcgagcgc ctcggcgagc gtgtccacgg acgcaatgcc
     8461 ttcgaacagg gcgtcgagcc cggccgggcc gcgcacgcgc gatcgcagcg cgccacccgc
     8521 tccgcccggg agcggcgctc cctgcccgcc cgcgaagaac gtcacggtcc ccgacgagag
     8581 gctcgcgagt gtgccggcaa cgtcgtcgat cgaatcgacg cataccgctt ccaggtacga
     8641 cccgagcacc gtctccacgg cgcgttccca gccgctgtcc acggcgagtt gctgcgcgag
     8701 gcgcgggttg ccattgagcc cgcgcgactc gagccactcg acgaccttgc cctgtccctg
     8761 ccccagtgcg gctttctgca gcgcctccag tgacacgacc cggccttcgg cctcctgcgc
     8821 gtcgcgccgc gcgccctgcg cggcttccgc cagctcacgc tcgcgggctc gctcggcctg
     8881 caggtccgcc accagccccg cgatccggtt gcgcgctgcc tcgagcgcgc cgcgcgccgc
     8941 cgactcggca cgatcgagct cgttgatctt cgctgcgacg tcggcggccg agagcacggc
     9001 gtgctcctgg gaggcacgct cccgctgctc ggcgagccgc ttcacgtgca cgtccatctc
     9061 gcccaggcgc gcacgctcgg cttccgccgc cgacgaagac tcccgcaacg actggttgaa
     9121 tgcttcccag cgctcctgcc acgcgtgcat cgccgcctcg gattccgcga ggcggttcgc
     9181 cgaactctcc tcggcgctgc gtgcggccac caggccgggc tcgatctccg cgagcaggag
     9241 cgcgagttcg gctgcctgcg cggcatcgcg cgcgcggtgt gcgcccgcct cctcgagtcc
     9301 ctgggcaacc tggcccagct cctgtcgctg gcggctcctt agctcgcgcg catgctcgat
     9361 ggcctgctcg gtgcgcgaga tgtcggcacc gacctggtag tagcggccct ggacctcgcg
     9421 gagcacttcg ccctgggcat cgtggtcgcg gcgggcgcgc tcgatggcag cctcgatcga
     9481 tcgctggccg gccagtgctg cctgcatcgc cagctcgcgc tcgctcatga tcgcctggcg
     9541 cgccccggcc tccgattcga cctcgcgcag acggagtgcg agcagttccg cgtgcaactg
     9601 ccgctcctcc tgcttcagcg cctggtagcg gcgcgcggtc gccgcctggc gctgtaggtg
     9661 ccgcaactgc ttctccactt cgtcgcgcag gtcggacagg cgggcgaggt tctcgcgcgt
     9721 gtggccgatg cgggactccg tctcgcgccg gcgctccttg tatttcgaga tccccgcggc
     9781 ttcctcgatg aacgcgcgca tctcgtcggt cttcgcctcg atcacgcgcg agatcatgtt
     9841 ctgctcgatg atcgcgtagc tgcgcgagcc gaggcccgtg cccaggaaca gctgcgtgat
     9901 gtccttgcgc cggcagcgcg tgccgttgag gaagtacgac gacgtcccgt cacgcgagac
     9961 gacgcgacgc agcgacacct cggcgtagtt cgcatactcc ccgccgagct tgccgtcggc
    10021 gttgtcgaac acgagctcga cggaggcgtg gccgaccggc ttgcgcgatc cagagccgtt
    10081 gaagatcacg tcggccatcg agtcgccgcg caggtgcttc gccgagatct cgcccatgac
    10141 ccagcgcacg gcgtcgatga tgttcgactt gccgcacccg ttcgggccca ccacgccgac
    10201 caggctgctc gggaaactga tcgtggtcgg gtccacgaac gacttgaatc cggccagctt
    10261 gatcttggtc aggcgcatcg atcacccagg gaaaaggggg ccacgggaac gagcgcgaag
    10321 tgtaaaagaa agtggccagc gccacagcac cgccggcgtc cccgaggcgg gccgcgaggg
    10381 gtcgctccgc acgctccccg ggcgggtgga ccggccgcgt cgggcctgta taatcccgcg
    10441 tccttcgcag tgtccccgga gtccgtagat gcccgcaaag aaaccgtctg ccaaggcgac
    10501 caaggccaag aagactgttt ctgcccgccc ggccagcaag gcgtcggcca aagccgcgcc
    10561 caaggccaag gccgcagcga ggaagacgcc cgcgaaggcg gtggtcaagc ccaaggccaa
    10621 ggcagccgcg cctgcgaaag ccgtggccaa gcccaaggca gctgcgaccg tgaaggcccg
    10681 cacggtgaca cccgctccgg cccagaaggc gccgcctgcg gcgaagcccg tggcagtcgc
    10741 ccccaggcct tcgactcctc cgccgtcacc gatcgcgtcc gccagcctgc tcgcgggacc
    10801 gcgcaacgtc gagccgtacg tcccgcgcaa gggcgaggag tacatgagca aggagcagct
    10861 cgagcacttc cgcgccatcc tgtcggactg gaagcaggac ctgatgcagg aagtcgaccg
    10921 cacgatgctg cacatgaagg acgaggcggc gaatttcccg gacccgaacg accgcgccac
    10981 gcaggagtcg gagttcagcc tggagctgcg cacgcgcgac cgcgagcgca agctgatccg
    11041 caagatcgac gaggccttga agcgcatcga ggacggcagc tacggcttct gcctcgagac
    11101 cggcgagccg atcggcgtga agcgcctcga ggcccggcca gtggccacgc tcagcgtcga
    11161 ggcgcaggaa cgccgtgagc ggcgcgaaaa gcagttcggc gaccgcgacg actggtaccg
    11221 ttgagggcgc aacgccctgt cgcgggcgat ttgcgccgac gccgagcggg gcgctgcatt
    11281 tcggttccct cgttgccgcc ctcgccagct ggctcgacgc gcgcgcacgt ggcgggcagt
    11341 ggctcgtccg catcgaggac ctcgatcccc cgcgcgagcg tcccggcgcc gcggacgaca
    11401 tcctcgccac gctcgaatca ctcggcctgc actgggacgg accggttctg cgccagagtt
    11461 cccgccacga cgcctatgac gccgcgctcg agcggctgcg ccgctacggc cggctgcgcg
    11521 agtgcgcctg cccccggacg gcactcgcga gcctgccgca gaacgctcag cgggcgtccg
    11581 gagacgatct ctaccatcct ccggaatgcg tcgtcggcgc cgggcaccga ggcggcatcg
    11641 cactgcgttt ccgggtgcct gatggagagg tcgaatttct cgaccgcgcg cagggcgcag
    11701 tgcgggtgaa cgtcgcgcgg tcggtcggcg atttcgtgct gcgccggcgc gatgggctgt
    11761 acgcctacca gctcgcggtc gtggtcgacg acgaggcaca gggcatcacc gacgtcgtcc
    11821 gtggggccga cctgctgtcg agcaccccgc gccaggtgct gctgcaggac gccctgggcc
    11881 tgcgccggcc gaaatacatg catgtcccac ttgccgtgga cgctggcggc cttaagcttt
    11941 ccaagacgtc ggacgcgccg cgactggcgg gggccgcgcc ggccgcgcaa ctggccgccg
    12001 cgctggactt cctcggccag gagccgccgc cggacgctgc gggtgcggcc gtcggtgacg
    12061 tcctgcgctg ggcagccgcg cactgggatc cggcacgatt cgcgggatcg acgtcacggg
    12121 caacgcccgc actggcacgg gattgatcag ggacagacaa caggtagaag acatggcaac
    12181 caaggctaag accaggcgaa cgcgcgcagc tgccaatgcg gacggcggcc gtccggcccc
    12241 acgtgtgatt cgcaagtatc ccaaccgtcg cctgtacgac accgtcgaga gccgctacgt
    12301 cacgctggcg gacatccgtc gcctcgtggt cgagcgcatc gacttcgtgg tgctcgaccg
    12361 caagaaccag caggacatca cgcgcagcat cctgctgcag gtgatcgcgg agcaggaagg
    12421 cgccggagag tcgctgatga gccgcgactt cctgtcgcac gtgatccgga cctacggcag
    12481 cggactgcag gatttcgtgg gccgctacct cgacgagagc atccgccagt tcgcgcgcga
    12541 gcagcgcgac ctgcgcgaca agctcaagag cgtggtcggc gtcgacccgg tcgagaccgt
    12601 cacggcggtc gcgcagaaga attaccagcg ctggaaggag ctgcaggaag aggtcttcag
    12661 ccgcctgccc ggttcctggg ggcgcgagaa ggaaaaggac aaggagtagg ccaggctggc
    12721 gacccgccgg acggcagcgg cagcaagaaa aagggccggc aatgccggcc ctttctcatt
    12781 catcgccatc gatcgccaag cgcgcgcgag cgcgcgcagc gtcacgcgtc gacgttgcgc
    12841 atcgacagcc gcacgcggcc ctgtcggtcg acctcgagca ccttcacgcg cacgacgtcg
    12901 ccttccctga gcttgtcgct cacccgctcg acgcgctcgt cggagatctg cgagatgtgc
    12961 accaggccgt ccttgccggg caggatctgc acgaatgcgc cgaagtccat caggcggacg
    13021 accttgccct cgtagatcgc gcccacctcg acctcggagg tgatgagctc gatgcgccgc
    13081 tgcgcctcgc gaccggccgc accgttgacc gacgcgatct tgacggtgcc gtcgttctcg
    13141 atgtcgatcg tggtgccggt ctcctcggtg atggcgcgga tcaccgcgcc acccttgccg
    13201 atgacctcgc ggatcttctc cgggtcgatc ttgatcgtga tgatcgtggg cgcccactcc
    13261 gacatctgcg ggcgcgcctc ggtgatgacc ttggccatct cgccgaggat gtgcaggcgc
    13321 gccttgtggg cgtgctcgag cgcgaccttc atgatctcgc gcgtgatgcc ctcgatcttg
    13381 atgtccatct gcagcgcggt gacgccgttc gacgtgccgg cgaccttgaa gtccatgtcg
    13441 ccgaggtgat cctcgtcgcc gaggatgtcg gtcagcacct ggaacttgcc gccctcgagc
    13501 acgagcccca tggccacgcc cgcgaccggc gccttgagcg gcacgccggc gtccatgagc
    13561 gagaggctgg tgccgcacac cgaggccatc gagctcgagc cgttcgactc gaggatctcg
    13621 gagacgacgc ggatcacgta ggggaacgtg gccatgtccg gcatcacggc gctgacgccg
    13681 cggcgcgcga ggttgccgtg gccgatttcg cggcgcttcg gcgagcccat catgcccgtc
    13741 tcacccaccg agaacggcgg gaagttgtag tggagcatga acggctcctt gcgctcgccg
    13801 gcgagcgcat cgatgagctg cgcgtcgcgg ccggtgccga gcgtggtcgt gacgagcgcc
    13861 tgcgtctcgc cacgggtgaa cagcgccgag ccgtgcgtgc gcggcagcac gccggtctgc
    13921 acggtgatct tgcggaccgt gtgcatgtcg cggccgtcga tgcgcggctg gcccgcgagg
    13981 atgcggccgc ggacgaggtt gtattcgagc atgccgagct ggtcgctcac cttgcgggca
    14041 tcgaatctgg ggctctcgcc gccggccagc gcctcgaccg tggccttctt gatctcgccg
    14101 atgcgggcgt agcgtttctg cttgtcggtg atcgtgtagg cctcgcccag cgcggcctcc
    14161 gcctgcgacg ccactgcggc gacgagcgcc gggtcgacgg ccggcgcgga ccacgcccag
    14221 gccggcttgc cggcctcggc agccagttcg cggatcgcct tgatcgcgac ctgcatctgc
    14281 tcgtggccga a
//
LOCUS       DAOMFD010000039        14259 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_981_length_14259_cov_35.999930, whole genome shotgun sequence.
ACCESSION   DAOMFD010000039 DAOMFD010000000
VERSION     DAOMFD010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 14259)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14259)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14259
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_981_length_14259_cov_35.999930"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /gene="prfA"
                     /locus_tag="PL152_03875"
     CDS             <1..209
                     /gene="prfA"
                     /locus_tag="PL152_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650806.1"
                     /note="recognizes the termination signals UAG and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF1; this protein is
                     similar to release factor 2; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQR48447.1"
                     /translation="QAQERKLQVGSGDRSERIRTYNYPQARVTDHRINLTIYQLPAVM
                     EGSLDELIGPLQQEWQAEQLASLQ"
     gene            229..1074
                     /gene="prmC"
                     /locus_tag="PL152_03880"
     CDS             229..1074
                     /gene="prmC"
                     /locus_tag="PL152_03880"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQR48448.1"
                     /translation="MASGIRALIEEGAARLSRVADDPRHEAAILLAAALGKPRSYLLA
                     HPDERVLDCDATDRFEAHVTRRSHGEPIAYILGEKEFWSLPLLVTPGVLVPRPETELV
                     VERAIAHVSRDMSADVLDLAAGSGAIALAIARERPRCRVMGTDDSADAVALAARNAAH
                     LGLGRVVLRQGHWFEPAAGQMFDVIASNPPYIAEGDPRVDAAVRRYEPHHALFSGATG
                     LEALAEIVKGAPRHLRHGGWIVLEHGDRQGVAVRQLLLDARFEQVATYRDLAGLDRVT
                     EGRWR"
     gene            complement(1071..2582)
                     /locus_tag="PL152_03885"
     CDS             complement(1071..2582)
                     /locus_tag="PL152_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR48449.1"
                     /translation="MRHRLDLLWRAAETPDAPAIETREVTWGFRELADRASRAGSFLS
                     NRAAQDGSPIALLMPSDERFVAWFHAVKLTGRPVMPLNTRLTAGELAQQLCDARATCL
                     VSDVADDRIAALSGLVPRLEIVVAPDLASLPDSPSGKTGERRGVAGETRGSGIDDSAI
                     VCAVIFTSGTTGRSKGACLTRANFTASAVAAAERLGPAVAGRWLACMPLFHVGGLSML
                     VRSVIFGGPVRLLPRFDAALVSDALDAGDVDAVSLVPTMLSRLLAHRGARRAPPGLRV
                     LLLGGASASPDLLTRACALGYPVCPTYGLTEATSQVATAAPPEPGAALPAPVRPLAGV
                     EVRIVDDAREVAPGEPGEILVRGATVTSGYLNDAAATSRAIRDGWLHTGDIGFVDFAG
                     ALHVLDRRDDLVVSGGENVYPAEVEAVLLGHPSVADAGVAGLPDADLGARLIAWIVVS
                     ETAVADAAALDAHCRRQLAGFKRPREFRFVEGLPRNAAGKLQRRDLAGLAFSR"
     gene            complement(2579..3631)
                     /gene="menC"
                     /locus_tag="PL152_03890"
     CDS             complement(2579..3631)
                     /gene="menC"
                     /locus_tag="PL152_03890"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: protein motif:HMM:TIGR01927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HQR48450.1"
                     /translation="MRLPFARPMRTSRGTFAERVSVILELRDGEGRRGYGEAAPWPGF
                     GTESAAAAHAALDAFSREAVGESLDAGDPSLETHACLGDAPAARAAARGALWDLEARI
                     AGLPLAAFLAARVHEASGPVLREVHVNGLLFERSPHALRAEAARLREAGYRAAKMKLG
                     TGSLQEEVELARAARAGLGPDVSLRGDANGAWNEVTAPIALETLADCGFEYVEQPVPP
                     GEVAALARLRGRTPVRIAADESVAGEHAASQLLAAAAVDVMVLKPAMVGGAERALEIA
                     ARAQRAGVSVVFSHAFESAVGACHALACAAAWGDAGTAHGLETAGLFVRDLADPVAAQ
                     GGVARVARTPGLGIEL"
     gene            complement(3658..4542)
                     /locus_tag="PL152_03895"
     CDS             complement(3658..4542)
                     /locus_tag="PL152_03895"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: protein motif:HMM:TIGR00751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046428 - 1,4-dihydroxy-2-naphthoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HQR48451.1"
                     /translation="MATVSARSAWWLAIRPATLTASAAPVLVGTGAAWADGVFSAGPA
                     LAALLGAALLQVGANFANDVFDFERGADNADRLGPQRATQQGWISAAQMKRAMWLAFA
                     GAGLVGIYLTAVAGWPVLAAGLLSIAAAYLYTGGPKPYGYLGLGDLSVFLFFGPVAVA
                     GTYFVQGLSFSPLVFIAAIPIGTLATAILAVNNLRDIETDARAGKLTLAVRLGDGPTR
                     GYYLLLLAIAYAAPPLLWWRGLAGPPVLLPWLTLPLVLRLASRMRHERGLALNGCLVQ
                     TARLEVVFGLLFALGLAW"
     gene            complement(4530..5375)
                     /gene="menB"
                     /locus_tag="PL152_03900"
     CDS             complement(4530..5375)
                     /gene="menB"
                     /locus_tag="PL152_03900"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016079909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HQR48452.1"
                     /translation="MIDTCRPAHDRPHWQAVRSYEDIRFEKDGGLARITICRPEVRNA
                     FRPKTLFELIDAFTRAREDSSIGVIIFTGEGDLAFCSGGDQRIRGDEGYVGDDGVPRL
                     NVLDLQRLIRSLPKPVIAAVAGYAIGGGHVLHVVCDLTIAADNARFGQTGPRVGSFDG
                     GFGSSYLARMVGQKKAREIWYLCEQYDAQEALAMGLVNKVVPLAELEATAVAWGRRIL
                     QHSPMAIRCLKAAFNAELDGQAGIQELAGHATQLFYMTEEAQEGRDAYNEKRPPDFSR
                     FPWRP"
     gene            complement(5372..6217)
                     /gene="menH"
                     /locus_tag="PL152_03905"
     CDS             complement(5372..6217)
                     /gene="menH"
                     /locus_tag="PL152_03905"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR03695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070205 -
                     2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HQR48453.1"
                     /translation="MTAGALHFERRGKGSPLMLLHGFTGSANSMRGLADGLARDFETI
                     APDLPGHGRSTAGADAGYSFDACLDALVATLRAAGHRRAHWLGYSMGARLALAVAVRH
                     PDSVASLVLLSGRAGIASEGERESRRRADETLARRIETEGVAAFVDDWMSMPMFQTQQ
                     RLGAAFLEEQRRDRLRNDAAGLAASLRALGPAAQPPLFDELSRVTVPVLLVVGTLDTA
                     FVAHAHELERRLSGAQLCEIADAGHAVHLERPSAVVAAIHDFLCRTDGPARSSHPPSV
                     QETGS"
     gene            complement(6214..8013)
                     /gene="menD"
                     /locus_tag="PL152_03910"
     CDS             complement(6214..8013)
                     /gene="menD"
                     /locus_tag="PL152_03910"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HQR48454.1"
                     /translation="MADRDGAGDVGSANVAFARALVDQLCAGGLAAAFVCPGSRSTPL
                     ALAIAESRGLPHWVHVDERSAAFHALGYARATDRPVALVCTSGTAGANFLPAVAEAFH
                     SRAPLVVLTADRPPELRAWGAAQTMEQRTLFAAFTRWSEEAPCPSERGGEPAYARALA
                     RRALHEATGPAPGPVHLNLPFREPLLPASIAVAEISRPPPEPRAVASRDPRAATGAAG
                     AVAAETLNAVAELADVERGVLVLGPDRWDPELAGAVHALAAALGWPVIADPASGLRAG
                     PMTSSSVIHGADLLLRDASTAARLRPDLVVRCGALPTSKALGEWIARHEAADVCLVDP
                     AGGFRDPQHRASRSLRLGSRQFCELACAMRGTTRPVPGSWLAQWRRADEVARAAAANA
                     IAAESRLFTPHVARALWETLPSGAALYVANSMPIRELDAFADPREGDLRVLANRGVNG
                     IDGLVSAACGASAALGGPAVLWCGDLALLHDVSGLLAGRMHGADLTIVVSNDDGGGIF
                     EYLPAARTLPRPMFERMFAVPHGLDLCAIARGLGWEASRVATAAELAASVSRSIAGGR
                     HLIEVSVDRAANTACHHAIHAAVGAALRRESAA"
     gene            complement(8013..9293)
                     /locus_tag="PL152_03915"
     CDS             complement(8013..9293)
                     /locus_tag="PL152_03915"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008909 - isochorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase"
                     /protein_id="HQR48455.1"
                     /translation="MSSARLAIRSEPCVAVDPLAVFAAASGESRFYWEQPAAGCFRVG
                     VGCARRLAVSGAGRFRAAEAGARDLFERIDWEGPGPRVGHLVGGFAFAPARSTSDLWR
                     GFTDGALVMPELAYWSDDGRCVRDACVDSRWAGYRPRPLALGQPVRGMVEIGNGGHEP
                     YETRVRGILDEIRANRLQKVVLSRAVHVTAGSTIDAVAWLVALRERFPSCTLFAVGEG
                     DAVFLGATPERLVRVQGRLVETAALAGTAPRGASRAADQALGVALCDSLKNGAEHAVV
                     VRFLSEALAECCDDVAVAPEPVLLKTRTVQHLCTPLSARRRSAAEPSLLELVERLHPT
                     PAVGGAPREGALRWLSDHEGIERGWFASPVGFLRSDGDGEFAVALRSALVRGRTATAW
                     AGAGIVARSEPRAEFTETELKLRTVLGPLLWGAP"
     gene            9610..10104
                     /locus_tag="PL152_03920"
     CDS             9610..10104
                     /locus_tag="PL152_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR48456.1"
                     /translation="MIRFETTLGSFTVELFEKEAPLSARNFLEYVDSGHFDGTVFHRV
                     IPGFVIQGGGLTADMRQKSTREPIRNEADNGLKNRRGTLSMARTNDPHSATSQFFVNL
                     VDNAFLDPGRGGAGYAVFGCVTEGMDVVDKIAAVKTGNKAGHQDVPVTPVEVKSARRI
                     AAKG"
     gene            10135..10854
                     /gene="mtgA"
                     /locus_tag="PL152_03925"
     CDS             10135..10854
                     /gene="mtgA"
                     /locus_tag="PL152_03925"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: protein motif:HMM:TIGR02070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HQR48457.1"
                     /translation="MAKDRKVRFSRVLRWIGLALLGWAGLTIAVVVALRWVDPPFTAF
                     MLEDRVRALVSRDPGYGFRQEWRDWDHISPNAALAVVASEDQNFPVHHGFDFEQIDKA
                     LAARERGRRVRGASTISQQVAKNLFLWPGQSWIRKGIEAGITVLIETAWSKQRILEVY
                     LNVAEFGRGTFGVQAASRRYFHKDAARLTRDEAALLAAVLPAPKRFRADAPSSYVRRR
                     QAWIERQMAGLGGTSYIQGLD"
     gene            10862..12322
                     /locus_tag="PL152_03930"
     CDS             10862..12322
                     /locus_tag="PL152_03930"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR48458.1"
                     /translation="MTTTTRIAIIGAGHSGICMGMLLKRAGIHDFVILEKAASLGGTW
                     RENTYPGASCDAPSFLYSFSFAQKTDWSRRFAWQPELLAYAAECAVGAGLLPHIRFRA
                     EVSAAAFDERRSIWHVDLADGTTVEAQFLVTGVGQLHRPSIPDLPGRRDFSGAQFHSA
                     QWDHSQDLTGRRVAVVGNAASAVQFVPQIAPVVSQLTIFQRSANWLLPRKDRLYSPRL
                     HRRMTRWPWLAKAYHDCQWFFFGEMMLTPLMKRVRFAQWAARARSLMHLRRQVKDPEL
                     RAKLVPDYPIGAHRVLFNDDYYPALGRRNVRLVTDAIASVESRGVRTRDGTLHEADVI
                     IYATGFKTTEFLAPMRITGLGGRALADEWRHGAHAYLGVAVSGFPNLFMLYGPNTNLG
                     HNSILVMIEAQAGYIVQAVRQAERSRLARLDVKRGVMDDYNRQLAADLAKSVWAASKA
                     SWYKLADGTITNNWPHSTIRYRRLMRTFDLDAYEVA"
     gene            12481..13362
                     /locus_tag="PL152_03935"
     CDS             12481..13362
                     /locus_tag="PL152_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48459.1"
                     /translation="MRIPTLVAGLALVTLAGCGKAPAPPAAEAPADTTAAAPATPADT
                     AAADAARAAELAAKEKELAEREAALKQQEVEQELARRNAEAAAAQQAAARKPAPKPAP
                     ARTTTASTQPQAKPVPPPPPPPLVAPAGTQLAIELTSPVSTKTATVGSAVQGRLASDL
                     VIGGRLAAAAGSTVTGTVTQVVSGSKKIGGTPTLGITFDRLVAGTGGPVAMSARFVQQ
                     AASDTGKDTAKVVGTTAAGAIIGHQVSHKNGAIVGGVLGGAAGLAIAQNTGGEVSLAS
                     GTVVTVSLDGSIQFPPG"
     gene            complement(13388..>14259)
                     /locus_tag="PL152_03940"
     CDS             complement(13388..>14259)
                     /locus_tag="PL152_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HQR48460.1"
                     /translation="YIDWMPFFNAWEFAGKFPDVLTDPVVGEAASNLYADARRVLKQM
                     IAERWVRASAVIGLFPANSADDDIEVYADESRGQVAWRLHHLRQQKPKPAGQPHFALA
                     DFVAPRDSGVKDWVGAFAVTAGIGLDEKVREFEARHDDYGAIMLKALADRLAEALAER
                     MHERVRREFWGYAPEERFTSDDLVREKYRGIRPAPGYPACPDHTEKATLWRMLDVERN
                     AGIRLTESYAMHPTAAVSGWYFSHPEATYFAVGKIDADQVADYARRKGMSVKDAERWL
                     APLIGYEPAVDAA"
BASE COUNT         2235 a         5244 c         4872 g         1908 t
ORIGIN      
        1 agcaggccca ggagcgcaag ctgcaggtcg gcagcggcga ccgctccgag cgcattcgca
       61 cctacaatta tccgcaggcg cgggtcacgg accaccgcat caacctgacc atctaccagc
      121 tgccggcggt gatggagggt tcgctcgacg agctcatcgg cccgctgcag caggagtggc
      181 aggccgagca actggcgagc ctgcagtgag cggcgaagcc gtgatgcaat ggcgagcggt
      241 atccgggcac tgatcgagga gggcgccgcc cgcttgtcgc gcgtggccga cgatccgcgc
      301 cacgaggccg ccatcctgct ggccgccgcg ctcgggaagc cacggtcgta tctcctggcg
      361 caccccgatg agcgggtgct cgactgcgac gccacggacc gattcgaggc ccacgtcacc
      421 cgccgttcgc acggcgagcc catcgcatac atcctgggcg agaaggaatt ctggtccctg
      481 ccgctcctcg tcacgccggg cgtactggtc ccgcggccgg aaaccgaact cgtcgtcgaa
      541 cgcgccatcg cccacgtgtc gcgcgacatg tcggctgacg tgctcgacct cgcggccggc
      601 agcggcgcga tcgcgctcgc aatcgctcga gaacgcccgc gctgccgggt catgggcacg
      661 gacgattccg ccgatgcggt cgcgctggct gcgcgcaatg ccgcgcacct cggcctcggc
      721 cgcgtcgtgc tccgccaggg ccactggttc gagccagcgg cgggacagat gttcgatgtc
      781 atcgccagca acccgcctta catcgccgaa ggcgatcccc gggtcgacgc cgcggtgcga
      841 cgctacgagc cccaccatgc gctgttctcg ggcgcgaccg ggctcgaggc cctggcggag
      901 atcgtcaagg gcgcgccgcg ccaccttcgc cacggcggct ggatcgtgct cgagcatggc
      961 gaccggcagg gtgtggccgt gcggcaattg ctgctcgacg cgcgattcga acaggtcgcc
     1021 acgtaccgcg acctcgccgg gctcgaccgc gtcaccgagg gccgctggcg ctagcgcgaa
     1081 aacgccagcc cggccaggtc gcggcgctgc agcttgccgg ccgcattgcg cggcaaccct
     1141 tcgacgaagc ggaattcgcg cggccgcttg aatcctgcga gctgtcggcg gcaatgcgcg
     1201 tcgagcgcgg cggcatccgc gaccgcagtc tcgctcacga cgatccaggc gatcaggcgt
     1261 gcgccgaggt ctgcatccgg aaggccggcg acgcccgcat cggccaccga agggtgcccg
     1321 agcagcaccg cttcgacctc cgcgggatag acgttctcgc ctccggacac cacgagatcg
     1381 tcacggcggt cgagcacgtg cagcgctccc gcgaaatcca cgaacccgat gtcccccgtg
     1441 tgcagccagc cgtcacggat ggctctcgac gtcgcggccg cgtcgttcag gtatccggag
     1501 gtcacggtcg cgccccgcac cagtatctcg cccggctcgc ccggcgcgac ttcccgcgca
     1561 tcgtcgacga tgcgcacctc gacgccggcg agtggtcgca cgggtgccgg gagtgcggcg
     1621 ccgggttcgg gcggcgccgc ggtggcaacc tgggaggtgg cctcggtcaa gccgtacgtc
     1681 gggcacaccg ggtaccccag cgcacaagca cgggtcaaca agtccggcga cgccgatgcg
     1741 ccgccgagca ggagcacacg caggcccgga ggtgcccggc gcgccccgcg gtgggcgagc
     1801 agtcgcgaca gcatcgtcgg caccagcgac accgcgtcga catcgccggc atccagtgcg
     1861 tcgctcacga gcgccgcatc gaatctcggg agcagtcgca cgggcccgcc gaagatgacg
     1921 ctgcgcacga gcatcgagag tccgcccacg tggaagagcg gcatgcacgc gagccatcgg
     1981 cccgccacgg cggggccgag ccgctccgcc gcggcaaccg cgcttgcagt gaagttcgct
     2041 cgcgtcaggc acgcgccctt ggaccgaccc gtcgtgccgg acgtgaagat cacggcacac
     2101 acgatcgcac tgtcatcgat gccagaaccg cgcgtctccc ctgcgacgcc acgccgctcg
     2161 cccgtcttcc cgctggggga gtccggcaag ctcgcgagat cgggcgcgac cacgatttcc
     2221 agccgcggca cgagacccga cagcgcggcg atccggtcgt cggccacgtc gctgaccagg
     2281 caggtggcgc gggcatcgca cagctgctgc gcaagctcgc cggcggtcag gcgcgtgttg
     2341 agcggcatga cgggacgacc cgtgagcttg accgcgtgga accacgcgac gaaccgctca
     2401 tcgctcggca tcaggagcgc gatcggactc ccgtcttgcg ccgcccgatt cgacaggaac
     2461 gagcccgccc gggacgcgcg gtccgccagc tcgcggaaac cccacgtgac ctctcgagtc
     2521 tcgatcgcag gtgcatccgg ggtctcggct gcacgccata acaggtcgag tcgatgcctc
     2581 acagctcgat cccgagtccc ggtgtgcgcg caacgcgcgc caccccgccc tgcgctgcca
     2641 cggggtcggc gagatcgcgc acgaacagtc ccgccgtttc gagtccgtgc gccgtgccgg
     2701 cgtctcccca cgcggcggcg caggcgagtg cgtggcacgc accgacggcg ctctcgaagg
     2761 cgtgcgagaa cacgacgctc acgccggccc gctgcgcgcg agctgcgatc tcgagtgcgc
     2821 gctctgcgcc gccgaccatc gccggcttca gcaccatgac gtcgacggcc gcggctgcga
     2881 gcagctgcga cgcagcatgc tcgccggcga cggactcgtc ggcagcgatc cggaccggcg
     2941 ttcgaccacg cagccgcgcg agcgccgcga cttcgcccgg gggcaccggt tgctccacgt
     3001 actcgaaccc gcagtcggcc agcgtctcga gtgcgatggg cgcggtgact tcattccacg
     3061 cgccgttcgc gtcgccccgc agggacacgt cgggcccgag acccgcgcgg gccgcgcggg
     3121 cgagctcgac ctcctcctgc agcgatccgg tgccgagctt catcttggcc gcgcgatacc
     3181 cggcctcgcg cagcctggcc gcctcggcac gcagcgcgtg cggcgagcgt tcgaacagca
     3241 gcccgttgac gtgcacctcg cgcagcaccg ggccactggc ctcatggacc cgggccgcaa
     3301 ggaatgccgc cagcggcaag ccggcgatgc gggcctcgag gtcccagagc gctcctcgcg
     3361 cggcggcacg ggcggccggc gcgtccccga ggcacgcatg cgtctcgagc gacggatcac
     3421 ccgcgtccag gctctcgccc accgcctcgc gcgagaatgc atcgagcgcc gcgtgcgcag
     3481 cggccgcgct ctccgttccg aacccgggcc acggggcagc ttcgccatag ccgcgccggc
     3541 cctcgccgtc gcgcagttcc aggatgacgc tgacgcgctc ggcaaacgtg ccacgcgaag
     3601 tgcgcatcgg gcgcgcgaat ggcaaccgca cgctccatgc tcgggcggac gcgatcctca
     3661 ccacgccagg ccgagcgcga acagcaggcc gaacacgact tcgagccggg cggtctgcac
     3721 gaggcacccg ttcagggcca gccctcgctc gtgccgcatg cgactcgcca ggcgcagcac
     3781 cagcggcagt gtcagccacg gcagcagcac cggcggaccg gccaggccgc gccaccagag
     3841 cagcggcggc gcggcatagg cgatcgccag caacagcagg tagtagccgc gcgtcggccc
     3901 gtcgccgagg cgcacggcca gcgtgagctt gcctgcacgc gcgtccgtct cgatgtcgcg
     3961 caagttgttc acggcgagga tcgccgtcgc cagcgtgccg attggaatcg cggcaatgaa
     4021 caccagcggt gagaacgaca gcccctgcac gaaatacgtg cccgcgacgg cgaccggccc
     4081 gaaaaacagg aacacgctga ggtcaccgag acccaggtac ccgtacggct tcggtccgcc
     4141 ggtgtagagg taggcggccg cgatcgacag caggccggcc gcgagcaccg gccagccggc
     4201 gacggcggtg aggtagatgc cgaccagccc ggctcccgcg aacgcgagcc acatcgcgcg
     4261 cttcatctgc gcggcgctga tccatccctg ctgggtcgcg cgctgcgggc cgaggcggtc
     4321 ggcattgtcc gcgccgcgct cgaagtcgaa cacgtcattc gcgaaattcg ccccgacctg
     4381 cagcagcgcg gcaccgagga gtgcagcgag cgcgggccct gcggaaaaca cgccgtcggc
     4441 ccacgccgcg ccggtcccca cgagcaccgg tgccgccgac gccgtcagcg tcgccgggcg
     4501 gatcgcgagc caccaggccg agcgggcgct tacggtcgcc atgggaagcg cgagaagtcc
     4561 ggcgggcgct tttcgttgta tgcgtcgcgc ccctcctgtg cctcctcggt catgtagaac
     4621 aactgcgtcg catgaccggc gagctcctgg atgccggcct ggccgtcgag ctccgcgttg
     4681 aacgccgcct tcaggcagcg aatggccatc gggctgtgct gcaggatgcg ccttccccag
     4741 gccaccgcgg tggcctccag ttcggccagc ggcacgacct tgttcacgag gcccatcgca
     4801 agtgcttcct gggcatcgta ctgctcgcac aggtaccaga tctcgcgcgc cttcttctgc
     4861 cccaccatgc gcgcgaggta cgacgacccg aagcctccgt cgaacgatcc caccctcgga
     4921 ccggtctggc cgaagcgcgc gttgtccgcc gcgatggtga ggtcgcacac gacgtgcagc
     4981 acgtggccgc cgccgatcgc gtaaccggcg acggccgcga tgaccggctt cggcagcgag
     5041 cgaatgagac gctgcaggtc gagcacgttc aagcgcggca cgccgtcgtc gccgacgtag
     5101 ccctcgtcac cgcggatgcg ctggtcgccg cccgagcaga acgccaggtc gccctcgccg
     5161 gtgaagatga tcacgccgat cgagctgtcc tcgcgcgcgc gcgtgaatgc atcgatcagc
     5221 tcgaagagtg tcttcggacg gaatgcgttg cggacctccg gcctgcagat cgtgatcctg
     5281 gcgaggcctc cgtccttctc gaagcggatg tcctcgtagc tgcgcaccgc ctgccagtgc
     5341 gggcggtcgt gtgcgggcct gcaggtgtcg atcatgagcc ggtctcctga accgaaggcg
     5401 ggtgactgga tcgtgccgga ccgtcggtcc gacagaggaa atcgtggatg gccgcgacga
     5461 cggcggacgg ccgctcgaga tgcaccgcat ggccggcatc ggcgatttcg cagagctgcg
     5521 cgccggacag tcggcgctcg agttcatgcg cgtgcgcgac gaaggcggtg tcgagggtgc
     5581 cgacgacgag cagcaccgga acggtgacgc gcgacagttc gtcgaacaac ggcggttgcg
     5641 cggccggacc cagcgcgcgc aggctcgccg cgagccccgc ggcatcgttg cgcaggcggt
     5701 cgcgacgctg ctcctcgagg aatgccgcgc cgaggcgctg ctgcgtctgg aacatcggca
     5761 tggacatcca atcgtccacg aaggcggcca cgccctcggt ctcgatgcgg cgtgccagcg
     5821 tctcgtccgc acgacgacgc gattcgcgtt ccccctcgga cgcgatcccg gccctgccgc
     5881 tcaacagcac cagcgacgcc acgctgtccg ggtggcggac cgcaaccgcc agcgccagtc
     5941 gcgcacccat ggaatagccg agccagtggg cacgccggtg gccggctgcg cgcagggtcg
     6001 cgaccagcgc gtcgagacac gcatcgaacg agtagccggc atccgcgccc gcggtcgagc
     6061 gaccgtgacc cggcaggtcc ggtgcgatcg tctcgaagtc ccgggcgagc ccgtccgcga
     6121 gtccgcgcat ggaattcgcg ctgcccgtga atccgtgcag cagcatgagc ggggagccct
     6181 tcccacggcg ctcgaagtgc agcgcgccag cagtcacgca gccgactccc gccgcagtgc
     6241 cgcaccgacc gcagcgtgga tggcgtggtg gcaggccgtg ttggcggcgc ggtccacgga
     6301 cacctcgatc aagtgacgac cgcctgcgat ggagcgggat accgacgccg caagctccgc
     6361 agcggtcgcc acacggctcg cctcccaacc gaggccacgc gcgatcgcac acaggtcgag
     6421 gccgtgcggc accgcgaaca tgcgttcgaa catcggccgc ggcagcgtgc gtgccgccgg
     6481 caggtactcg aagatgccgc cgccgtcgtc attgctgacg acgatcgtga ggtctgcgcc
     6541 gtgcatgcgc ccggccagca gccccgagac gtcgtgcagc aacgccaggt cgccgcacca
     6601 cagcaccgcc gggccaccga gcgcggccga ggcgccgcaa gccgcggaca ccagcccgtc
     6661 gatgccgttg acaccgcgat tcgcgaggac ccgcaggtcc ccttcccgcg ggtccgcgaa
     6721 ggcgtccagt tcacggatcg gcatgctgtt cgccacgtag agagccgcgc cggaaggcaa
     6781 cgtctcccag agtgcgcgtg ccacgtgagg cgtgaagagg cggctttccg cagcgatcgc
     6841 attcgcggca gcggcccgcg cgacctcgtc cgcgcgtcgc cattgcgcga gccagctacc
     6901 cggcacgggc cgcgtcgtgc cccgcatcgc acacgccagt tcgcagaatt gccgcgaacc
     6961 gagccgcagg gagcgactgg cgcgatgctg cgggtcgcga aatccgccgg ccggatcgac
     7021 caggcagacg tccgccgcct cgtggcgggc aatccactcg ccgagagcct tgctcgtcgg
     7081 caaggcaccg cagcgcacga cgaggtcggg acgcaggcgc gcggccgtcg atgcgtcgcg
     7141 cagcagcagg tccgcgccat ggatcacgga cgacgaggtc atcgggccgg cacgcaggcc
     7201 ggacgcggga tccgcgatca ccggccagcc caaggccgct gcaagggcgt ggaccgcgcc
     7261 cgcgagctcc gggtcccagc ggtcgggccc gagcaccagc acgccgcgct cgacgtcggc
     7321 gagttctgcg accgcgttca aggtctcggc agcgactgca cccgccgcac cggttgcggc
     7381 gcgcggatcg cgcgacgcaa ctgcacgcgg ctccggaggc ggcctcgaga tctcggcgac
     7441 ggcaatggat gccggaagca gcggctcgcg aaacggcagg ttcaggtgca ctgggcccgg
     7501 cgccggtccg gtcgcctcgt gcagggcgcg ccgcgcgagc gctcgcgcgt acgcgggctc
     7561 gccaccccgc tccgaagggc acggtgcctc ctcgctccag cgcgtgaatg cggcgaacag
     7621 cgtgcgctgt tccatcgtct gcgcagcgcc ccaggcgcgc agttccggcg ggcgatccgc
     7681 cgtcaacacc accagcggcg cgcgagaatg aaaggcctcg gcgaccgccg gcaggaagtt
     7741 cgcaccggcc gtgccggacg tgcacacgag cgcaaccggt cggtcggtcg ccctcgcgta
     7801 gccgagcgcg tggaaggcgg cgctgcgctc gtcgacgtgc acccagtgcg gcaagccccg
     7861 tgattccgcg atggcgagcg ccagtggcgt cgagcgcgat cccgggcaga cgaacgccgc
     7921 cgcgagcccg ccggcgcaga gctggtcgac gagcgccctc gcgaatgcaa cgtttgcgga
     7981 accgacgtcg ccagcgccgt ctcgatcggc catcagggcg ctccccacag cagcggaccc
     8041 agcacggtcc tgagcttgag ctccgtctcg gtaaattcgg cccggggttc cgaacgggcg
     8101 acgatccctg cgcccgccca cgcagttgcg gtgcggccgc gcaccagggc cgacctcaag
     8161 gctaccgcaa attccccgtc accgtcggac cgcaggaatc cgaccgggct ggcgaaccag
     8221 ccacgctcga tgccttcgtg atccgagagc cagcgcaacg cgccttcacg cggcgcgccg
     8281 ccgacggcgg gcgtcggatg cagacgctcg acgagttcga gcagggaggg ctccgcggcc
     8341 gaccggcgcc ttgcgctcag cggcgtgcac aagtgctgca ccgtgcgggt cttgagcaac
     8401 accggctccg gcgccacggc gacgtcgtcg cagcattcgg cgagcgcttc gctcaggaac
     8461 cgcacgacga cggcgtgctc ggcgccattc ttgaggctgt cgcacaaggc aacgcccagg
     8521 gcctggtcgg cggcgcggga cgcacccctg ggagccgtgc ccgcgagcgc agcggtttcc
     8581 acgaggcggc cctgcacgcg gaccagccgc tccggggtcg ctcccaggaa tacggcgtcg
     8641 ccctctccga ccgcaaacag cgtgcacgag ggaaatcgct cgcgcagcgc caccagccac
     8701 gcgacggcgt cgatcgtcga cccggccgtg acgtgcacgg cccgtgagag caccaccttc
     8761 tgcagccggt tcgcccggat ctcgtcgagg atgccgcgca cgcgcgtctc gtagggttcg
     8821 tggccgccgt tcccgatctc gaccatgccg cgcacaggct gccccagcgc gagaggacgc
     8881 gggcgatacc cggcccagcg actgtcgaca cacgcgtcgc ggacgcaccg gccgtcgtcg
     8941 ctccagtacg cgagctccgg catcaccagc gccccgtcgg tgaatccgcg ccacaggtcg
     9001 ctggtcgaac gcgccggggc gaacgcaaat ccgccgacga ggtgcccgac gcgcggcccc
     9061 gggccttccc agtcgatccg ctcgaacagg tcgcgcgccc cggcctcggc cgcccggaat
     9121 cggccggcgc ccgacaccgc cagtcgccgg gcacatccca cgccgacgcg aaagcaaccc
     9181 gccgctggct gttcccaata gaaacgggac tcaccgctgg ccgcggcgaa caccgccaac
     9241 gggtccaccg cgacgcacgg ttccgagcga atggccagcc gcgcgctgct caacgggtct
     9301 cccgacggcg gcccctcgca ccggggcccc gtggcatgcc gtaccgaatc gagcagaccc
     9361 gcatcactgc cttcatgtgc tccaggttcg cagtcgccgg tggccgccac gcacggcgaa
     9421 ttcgcttcga gtcgcgcctc ggctgcctcg cccgggccgg caacagcccg acactttacc
     9481 cgttggggcc gcgtccgggc gaacccgcgt ccggcggatt cgccagggtc ccaggcccga
     9541 ggggagtgac atcgcgcccc cgtccattca gtattccgtc ccctcccgcc acccacacac
     9601 ggacccgcca tgattcgctt cgagaccacc ctgggcagct tcaccgtcga gctcttcgag
     9661 aaggaagcgc ccctcagcgc gcggaatttc ctcgagtacg tggactccgg ccacttcgac
     9721 gggacggtgt tccaccgcgt gatcccgggt ttcgtgatcc agggcggcgg gctcacggcc
     9781 gacatgcgcc agaagtcgac gcgcgaaccg atccgtaacg aagccgacaa cggcctcaag
     9841 aaccgccgcg gcacgctgtc catggcgcgc accaacgacc cgcacagcgc gacgtcgcag
     9901 ttcttcgtga acctcgtgga caacgccttc ctcgacccgg gtcgtggcgg cgccggctac
     9961 gcggtgttcg gctgcgtcac cgagggcatg gacgtcgtcg acaagatcgc cgcggtcaag
    10021 accggcaaca aggcggggca ccaggacgta ccggtcacgc cggtcgaggt caagagcgcg
    10081 cggcggatcg ccgcgaaagg ctgaggcggc cagcagtctc cggcgccggt tgccgtggcg
    10141 aaggatcgca aggtccggtt ctcgcgcgtg ctgcgctgga tcggcctcgc cctgctgggc
    10201 tgggccggcc tcacgattgc cgtcgtagtc gcgctgcgct gggtggaccc gccattcacg
    10261 gcgttcatgc tcgaggaccg ggtgcgggcc ctcgtgtccc gggatcccgg atacgggttc
    10321 cgccaggaat ggcgcgactg ggaccacatc tcgccgaatg ccgcactcgc cgtggtcgcg
    10381 tcggaggatc agaatttccc ggtacaccac ggcttcgatt tcgagcagat cgacaaggcg
    10441 ctcgcggcgc gggagcgcgg ccggcgcgtg cggggcgcga gcacgatctc gcagcaggtg
    10501 gccaagaacc tgttcctgtg gcccggccag agctggatcc gcaagggcat cgaggcgggc
    10561 atcaccgtgc tgatcgagac ggcgtggagc aagcagcgca tcctcgaggt gtacctgaac
    10621 gtcgccgagt tcggacgcgg cacttttggc gtgcaggccg cgagccgccg gtatttccac
    10681 aaggacgctg cgcgcctcac gcgcgacgag gcggcactgc tcgcggcggt cctgcccgcg
    10741 ccgaagcgat tccgcgccga tgcgccgtcg tcttacgtgc ggcggcgaca ggcgtggatc
    10801 gagcgccaga tggccgggct cggcggcacg tcgtacattc agggactgga ctgagggccc
    10861 cgtgaccacc acgacccgca tcgcgatcat cggcgccggc cactccggca tctgcatggg
    10921 aatgctgctc aagcgggccg gcatccacga cttcgtgatc ctcgagaagg ccgcgagcct
    10981 cgggggcacg tggcgcgaga acacctaccc cggcgcgtcg tgcgacgccc cctcgttcct
    11041 gtacagcttc tccttcgcgc agaagaccga ctggagccgc cgcttcgcct ggcaaccgga
    11101 attgctcgcc tacgcagccg agtgcgcagt cggcgcaggg ctgctgccgc acatcaggtt
    11161 ccgcgccgag gtgagcgccg ctgccttcga tgagcggcgg agcatctggc atgtcgacct
    11221 cgccgatggc acgaccgtgg aggcgcagtt cctcgtgacg ggcgtggggc agttgcaccg
    11281 tccttcgatc ccggacctgc cgggccgccg ggacttcagc ggcgcgcagt tccattcggc
    11341 gcagtgggat cactcgcagg acctcaccgg caggcgcgtc gcggtcgtcg gcaatgcggc
    11401 gagcgccgtg cagttcgtgc cgcagattgc gcccgtcgtg tcgcaactga cgatattcca
    11461 gcgctcggcg aactggctgc tgccgcgcaa ggaccgcctg tactcgccgc gcctgcaccg
    11521 ccggatgacg cgctggccgt ggctcgccaa ggcctaccac gactgccagt ggttcttctt
    11581 cggcgagatg atgctgacgc ccctcatgaa gcgtgtccgg tttgcgcagt gggcggcgcg
    11641 cgcccggtcg ctcatgcacc tgcgcaggca ggtgaaggat ccggaattgc gcgcgaagct
    11701 ggtgccggac tatccgatcg gcgcgcatcg ggtgctcttc aacgacgact actacccggc
    11761 cctcggccga cgcaacgtgc gtctggtgac ggacgcaatc gcgtcggtcg aatcgcgtgg
    11821 cgtccgcacc cgggacggca cgctgcacga ggcggacgtg atcatctacg cgacgggctt
    11881 caagacgacc gagttcctcg cgccgatgcg gatcacgggg ctgggcggcc gcgcgctggc
    11941 cgacgaatgg agacacggcg cgcatgccta cctcggcgtc gccgtcagcg gattcccgaa
    12001 cctcttcatg ctctacggcc cgaacaccaa cctcgggcac aacagcatcc tggtgatgat
    12061 cgaggcccag gccggctata tcgtccaggc tgtccggcag gccgaacgca gccggctcgc
    12121 gcgcctggac gtgaagcgcg gcgtgatgga cgactacaac cggcagctcg ccgcggatct
    12181 cgcgaagtcg gtctgggccg cctcgaaggc gagctggtac aagctggccg atggcacgat
    12241 caccaacaac tggccgcaca gcacgatccg ctaccgcagg ctcatgagga cattcgacct
    12301 cgatgcctac gaggtggcct gaagtcgccc ctgctacggc cggcctgtcc ggcaaacgcc
    12361 ggttccggta ccgtgacgcg cgtcgcgaca cgacgcacgc cgggaactcc ggcatggtcg
    12421 ccggtatcca atacactgca tcacccctgg agtactctct tgcatacacg atcgacacgc
    12481 atgcggatcc cgaccctggt cgctggtctc gcactggtca cactcgccgg ctgcggcaag
    12541 gcgccggcgc ctccggctgc cgaagcaccc gccgacacga cggccgccgc accggcgaca
    12601 cccgccgata ccgctgcggc cgacgctgcg cgcgccgcgg aactcgctgc gaaggagaag
    12661 gagctcgcgg aacgcgaggc ggccctgaag cagcaggagg tcgagcagga gctcgcgcgt
    12721 cgtaacgccg aagcggcggc ggcacagcag gccgccgcgc ggaagcccgc cccgaagccc
    12781 gcgccggcac ggacgaccac cgcgtccacg cagcctcagg cgaagccggt cccacccccg
    12841 cctccgccac cgctcgtcgc gccggcaggc acccaactcg ccattgaact gacctcgcct
    12901 gtcagcacca agaccgcgac cgtcggcagc gccgtgcagg gccggctcgc atcggatctc
    12961 gtgatcggcg gccggctcgc cgcagccgca ggctcgaccg tcacgggcac ggtcactcag
    13021 gtcgtctccg gcagcaagaa gatcggcggc acgccgacgc tcggcattac gttcgatcgc
    13081 ctcgtcgcgg gcaccggcgg cccggtcgcg atgagcgccc ggttcgtgca gcaggcggcc
    13141 agtgacaccg gcaaggacac ggccaaggtc gtgggcacga cggcggccgg cgcgatcatc
    13201 ggccaccagg tgagccacaa gaacggcgcc atcgtcggcg gcgtgctcgg cggtgccgcc
    13261 ggcctcgcga tcgcccagaa caccggcggt gaggtgagtc tcgcgtccgg caccgtagtc
    13321 acggtgtcgc tcgacggttc catccagttc ccgcccggct gagcacggac cgcggtcggc
    13381 gctgccctca ggcggcgtcg accgcgggct cgtagccgat caacggcgcg agccagcgct
    13441 cggcatcctt caccgacata cccttgcgac gcgcgtagtc ggccacctgg tcggcgtcga
    13501 tcttgcccac ggcgaaatag gtcgcctcgg ggtgcgagaa ataccatccg ctgacggcgg
    13561 ccgtcgggtg catcgcgtag ctctcggtga ggcgaatgcc cgcgttgcgc tccacgtcga
    13621 gcatgcgcca cagcgtggcc ttctccgtgt ggtccgggca ggccgggtag cccggcgccg
    13681 ggcggatccc gcggtacttc tcgcgcacga ggtcgtcgct ggtgaagcgc tcctcgggcg
    13741 cgtagcccca gaattcgcgc cgcacgcgct cgtgcatgcg ctcggccagc gcctcggcca
    13801 gccggtcggc aagggccttc agcatgatcg cgccgtaatc gtcgtggcgc gcctcgaact
    13861 cgcgcacctt ctcgtcgagg ccgatccccg cggtgacggc gaacgcgccg acccagtcct
    13921 tcacgcccga gtctcgcggc gcgacgaagt cagccagcgc aaagtgcggc tggcccgcag
    13981 gcttcggctt ctgctgccgc aggtggtgca ggcgccacgc gacctggccg cgtgactcgt
    14041 ccgcgtagac ctcgatgtcg tcgtccgcgg aattcgccgg gaacaggccg atcaccgcgc
    14101 tcgcccgcac ccaacgctcg gcgatcatct gcttgagcac gcgtcgcgcg tccgcgtaca
    14161 ggttgctggc cgcctcgccg accacgggat cggtgagcac gtcggggaac ttgccggcga
    14221 actcccacgc gttgaagaac ggcatccagt cgatgtagc
//
LOCUS       DAOMFD010000040        13973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1023_length_13973_cov_33.298175, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000040 DAOMFD010000000
VERSION     DAOMFD010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13973
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1023_length_13973_cov_33.298175"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="PL152_03945"
     CDS             complement(<1..1102)
                     /locus_tag="PL152_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HQR48461.1"
                     /translation="MSFDQLGLSAALLRAVQELGYTEPTPIQQRAIPAVHDGRDLLAA
                     AQTGTGKTAAFALPLLQRLDAENPVPRGHRAPRLLVLVPTRELAAQVGESFEAFGRHL
                     QQRTLLIFGGVSPRPQVDALRHGVDIVVATPGRLLDHANERAIDLRHVRHLVLDEADR
                     MLDMGFIRDIRRVLALLPPNRQNLLFSATFSQEIRALATGLLKDPLTVEVAARNAPAE
                     LVEHRVHLVSQGEKRAVLSHLLREGATPQTLVFTRTKHGANRLAEQLEKDGIQCAAIH
                     GNKSQNARTKALADFKDYKVQVLVATDIAARGLDITELPQVVNYELPHVPEDYVHRIG
                     RTGRAGCAGQALSLVSDDERDRLHAIERVLGKK"
     gene            complement(1346..2980)
                     /locus_tag="PL152_03950"
     CDS             complement(1346..2980)
                     /locus_tag="PL152_03950"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR48462.1"
                     /translation="MQARSADLIIRGGPILTMEGAAPTYVEAVAVSAGKIVFAGTEAE
                     AMRRKGAATAVKELHGRTMLPGFVDAHSHFINAPALSRMVNVSPSPVGPGDTVASIVS
                     ALKAWQDKAKVPDGGWIMAWGYDDSMVQGGKLTRRELDAAFPNHKVLVLHVSLHGAVL
                     NSQALEWAGITRDTPTPDGGIIARGSDGKEPEGLLMETAFLPVFNKLPQPSEAEMLEL
                     LKPGQMMYAQNGYTLANDGFTHTRDIRLLQEAAAQGRLFIDIAVLPAFSEMDQWLGKP
                     EFPFGQWNGRLKLQGLKITQDGSVQGKTAYYIEPLLTGGPQGQPDWRGNTTLPYEQFA
                     KIFDAGHKAGLQVFVHANGDGAIQQVIQAAEAAGLKAGDDSRTIVIHSQMQHPEDIPR
                     YVALGLTPSYFTNHTFFWGDVHRRNIGETRAAFISPMRSAMDAGLKATNHSDFNVTPL
                     GPFFMLWTSMARTTRSGYVLGPDQRIDPYRGLQALTINPAWEYREETRRGSIAPGKLA
                     DFVIVSGDPLRTPVDRIRDIRVVETIKEGKTIYSRN"
     gene            complement(3166..5250)
                     /locus_tag="PL152_03955"
     CDS             complement(3166..5250)
                     /locus_tag="PL152_03955"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DPP IV N-terminal domain-containing protein"
                     /protein_id="HQR48463.1"
                     /translation="MKLSPDGRWITLLKNHPEDRERYDLWGYDRQTGQWSMLVDSARL
                     ESGRELSEAGKMQRERQRIGDLKGIVAYDWSDDGQSILVPLDGDLFLAGLDGKVTRLT
                     DTSEDELNPALSPKGRYVSFVRDGRLWTGPVRAAPAQPITPVEASDTVHWGEAEFVAQ
                     EEMDRTTGYWWSPDDSRVAVERYDEVPVGVVTRTAIGAEDTRTFEQRYPAAGTPNAVV
                     QLFVMDPDGSHRVEVDLGADKDIYLARVDWSPDGRTLYVQRQNRAQTRLDVLAVDPAT
                     GNSRVLFQERAAARSWINLSDSYKFLKDGSLVWWSERDGYGHLYQFRDGQWRQLTRGP
                     WVVTGLAGVDEDSGRVYFTANKDDVLAQQVYVLDLKTPDRIERLTEPGWWTRATMDEA
                     GKTLVVTRSSPDHPPQSYIADGTGKRLDWIEENRLDPSHPYAPYLASHRETSFGTIPA
                     ADGTPLHWMMITPPLEPGKRYPVFYDHYGGPTSQQVARAWSGALPQAIVDQGYIYFRL
                     DNRGSANRGVAFESTLFRAMGSVEVVDQLAGANYLKTLPFVDPAKVAIFGWSYGGYMT
                     IKMLQANPGVYAAGIAVAPVTRWELYDTHYTERYLGRPKTEPAVYATSDALADTAKIS
                     DPLLIIHGMADDNVVFEHSSAIIAKLQGEAVPFEMMLYPGYTHRISGPIVSKHLYETM
                     FRFLARNGVTPP"
     gene            5642..6688
                     /gene="pstS"
                     /locus_tag="PL152_03960"
     CDS             5642..6688
                     /gene="pstS"
                     /locus_tag="PL152_03960"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF008171.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HQR48464.1"
                     /translation="MNHRFLAVFALAAAALLGRPAFAADISGAGATFPYPIYAKWAEA
                     YKKETGIGLNYQSIGSGGGIKQIKAGTVTFGASDKPLEMKELTDSGLVQFPMIIGGVV
                     PVANVPGLKPGALKLDGPTLASMYLGEITKWDDARIKALNPGLSLPSLAIAPVYRSDG
                     SGTNFLFTYYLSGVSPGFKSKVGANTSVEWPVGIGAKGNEGVANMTTQTSGAIGYVEY
                     AFAKQNKMTYIVMKNRDGRFVSPEAKAFQAAAANADWMKAPGYYLILTDQPGAASWPI
                     TGASFILMYRNPKDANASRTALEFFAWAYAKGGDLAAQLDYVPMPPGVVKLVESTWQT
                     KITSGGQPVWSPKR"
     gene            6670..7638
                     /gene="pstC"
                     /locus_tag="PL152_03965"
     CDS             6670..7638
                     /gene="pstC"
                     /locus_tag="PL152_03965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HQR48465.1"
                     /translation="MVAEALSIQSLLRRERRVERAFRGLATSSAILVLALLSGVIIAL
                     AYDAWPALHEFGWSFVTETRWNPVTEKFGALAPLYGTVVTSIIAMALAVPVSFGIAIF
                     LTETCPVWLRAPIGTAIELLASIPSIVFGVWGLFVLAPVLQHTIQPWLIDHFGNLPVI
                     GFLFQGPPFGIGLLTAGLVLAIMVLPFISSVMREVFSITPVALKEAAYGLGATQWEVV
                     RGVVIPWSRPGIIGGLMLGLGRALGETMAVTFVIGNAHRITGSLLAPGTTISAAIANE
                     FTEAVGDMYTSALIALGLLLFVITFIVIAAARLLLMRLEASSGARE"
     gene            7635..8525
                     /gene="pstA"
                     /locus_tag="PL152_03970"
     CDS             7635..8525
                     /gene="pstA"
                     /locus_tag="PL152_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HQR48466.1"
                     /translation="MSAQRQQGTASAFGTRLQVRRRVVNVIGFALSGVATLIGLAVLA
                     ALLWTLVSRGVQSMSPDLFHLSTPAPGSEGGLANAIFGSVVMTMLGIAIGGPVGLLAG
                     TYLSEYGRGTVLASVIRFINDVLLSAPSIVIGLFVYTVVVMNAGHFSALAGALSLAII
                     ALPVMVRTSEDMLQLVPNSLREASAAMGANPWRTILATIYPAARNGLLTGLLLAVARI
                     SGETAPLLFTALNNQFWSADLNKPMANLPVVIFQFAMSPYEDWQGLAWAGALLITSFV
                     LVLSIGARLLAATARTRKSG"
     gene            8617..8940
                     /locus_tag="PL152_03975"
     CDS             8617..8940
                     /locus_tag="PL152_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003120011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR48467.1"
                     /translation="MKEPIVLKALAALAQVNRLRVFRALVVAGQAGATPGAIGRRLKV
                     SPATLSFHLKELVNAGLVTQERSGRNLIYRADYEAMNALLSYMTENCCQGMACDVPPE
                     RIRAR"
     gene            8950..9444
                     /locus_tag="PL152_03980"
     CDS             8950..9444
                     /locus_tag="PL152_03980"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQR48468.1"
                     /translation="MTTNVLILCTHNSARSVLAEAMLNHHAARLGLDVRGYSAGSAPS
                     GRVNPLALSVLEDAGVSTAGARSKSWDEFARSDAPHMRVVITVCDSAAGEACPVWPGS
                     PIRVHWGYADPSNAAGDAAARRVAFDLTRQAIGYRMLQLLALPLASMEDARLRQALLE
                     IARS"
     gene            9454..10527
                     /gene="arsB"
                     /locus_tag="PL152_03985"
     CDS             9454..10527
                     /gene="arsB"
                     /locus_tag="PL152_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004651694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008490 - arsenite secondary active
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACR3 family arsenite efflux transporter"
                     /protein_id="HQR48469.1"
                     /translation="MATVSRRLSFLDRYLTVWIFAAMAVGIAIGWLAPGVEAFVDRYT
                     VGTTNVPIALGLILMMYPPFAKVRYEDLPDVFRDKKVLALSLVQNWLVGPVLMFLLAI
                     TFLADKPEYMAGLILIGLARCIAMVIVWNELADGSTEYAAGLVAFNSIFQVLFYSVYA
                     WVFVTVLPPLFGLHGSVVDISIGQIARSVFVYLGIPCIAGVLTRVVMLRFVSKDWYHG
                     HFVPLISPITLAALLFTIVVMFSLKGKLIVTIPMDVVRIAIPLLIYFVVMFLVSFAMG
                     RRAGAAYPKCATLSFTAASNNFELAIAVAVAVYGIDSGAAFAAVIGPLVEVPVMIALV
                     NVALRFQQRWFGGPPAAGAPTRA"
     gene            10545..11360
                     /locus_tag="PL152_03990"
     CDS             10545..11360
                     /locus_tag="PL152_03990"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HQR48470.1"
                     /translation="MSIDIKQVVKERYGEAARKVISVKAEASCCGPAPSCCDPITSNL
                     YDAAQTRDLPEAAVLASLGCGNPTALAELQAGEVVLDLGSGGGIDVLLSAKRVGPTGK
                     AYGLDMTDDMLALANENKRKAGATNVGFLKGEIEHIPLPDASVDVIISNCVINLSADK
                     DQVLREAFRVLRPGGRFAVSDVVTRGEIEPQIRASVLAWVGCVAGALDEQVYRDKLAA
                     AGFTAIDVEPTRVYRVDDAKDFIAASGLDAAALAPKVDGKFMSAFVRARKPLA"
     gene            11386..12375
                     /locus_tag="PL152_03995"
     CDS             11386..12375
                     /locus_tag="PL152_03995"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQR48471.1"
                     /translation="MSEPAYLPFLQQTLRYFDRPHDGPALTPVSSPAAWRRGDLADAR
                     WRVRLTDDEVAELRRAVAAWLARSLPLAAMTKSDFPLPTLAARIRDWRDEVANGLGLQ
                     VISRVPVEAWSLEEASAFFWGLGQHLGRPGAQNRKGDLLGQVRDTGADRTDRYVRQYM
                     TTQDIPFHCDAADMVGLLCLRTAKSGGLSRIASSVSVYNELLRRRPDLASRLFEPFML
                     DTRDEAEANGVKALPIPPCRYGGGVLRTFWHSEYFRTAQRHEGILLTDAEHELLDLYD
                     EISASPEFHIDMALEPGDVQLLSNHTVIHARTDYEDYPEEDRKRHLLRLWITL"
     gene            complement(12600..12971)
                     /locus_tag="PL152_04000"
     CDS             complement(12600..12971)
                     /locus_tag="PL152_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR48472.1"
                     /translation="MITFPRVHTDELGETHIGVRELPRHDAALGPPPNPVGRMTDPIM
                     VSTLVTYSAPAGAGATWHTAPQPYICIVLSGEGEVVASDGTTRRLVPGDVMFFDDVTG
                     KGHITRAITDLHVALINRSGA"
     gene            complement(13092..>13973)
                     /locus_tag="PL152_04005"
     CDS             complement(13092..>13973)
                     /locus_tag="PL152_04005"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQR48473.1"
                     /translation="YWHTLSGANAVPLIVTSMSGNTNPFDPALTVVPGADFESTLILA
                     GYARTFTLFDRSAMGAVLLPMGRISGDVIAPGGTPVSQSVSGFGDPMVEFTLNVIGPK
                     AQENLQDVSRFEPGFSLDVLADLAIPIGEYDSSQPLNIGQNRWYGRIGFPIVWQIGEW
                     VPTRRTTLEFLPAVWLFGDNTDYLGQTLEADPLYQIDAHLTRDFTASLWGSLDAVYYN
                     GGKTTIDGVEGSKLNNYGFGFTLGYQINDNIGLTFSYKSTASDQAPDDLQMDVIMISL
                     VAGWHPIIEGFKRLQSE"
BASE COUNT         2192 a         4677 c         4648 g         2456 t
ORIGIN      
        1 tcttcttgcc gagcacccgc tcgatggcgt gcaggcggtc gcgctcgtca tcggaaacga
       61 gcgacagcgc ctgtcccgcg cagccggcgc ggccggtgcg gccaatgcgg tgcacgtagt
      121 cctcggggac gtgcggcagc tcgtagttca ccacctgcgg caactcggtg atgtcgaggc
      181 cgcgagccgc gatgtccgtc gcgaccagca cctgcacctt gtagtccttg aagtcggcca
      241 gcgccttggt gcgcgcattc tgcgacttgt tgccgtggat cgccgcgcac tggatgccgt
      301 ccttctcgag ctgctcggcg aggcggttcg cgccgtgctt ggtgcgcgtg aagaccaggg
      361 tctgcggagt tgcgccttcg cgcagaaggt gactcagcac cgcccgcttc tcgccttgcg
      421 agacgagatg cacgcggtgc tcgacgagct cggccggggc attgcgcgcc gcgacctcga
      481 ccgtcagtgg atccttgagc aggcccgtcg ccagcgcacg gatctcctgc gagaacgtcg
      541 ccgagaacag caggttctgc cggttcggcg gcagcagcgc cagcacgcgg cggatgtcgc
      601 ggatgaaacc catgtcgagc atgcggtccg cctcgtcgag gacgagatgg cgcacgtgcc
      661 tcaggtcgat cgcgcgctcg ttggcgtgat cgagcagccg gcccggagtg gcgacgacga
      721 tgtcgacgcc gtggcgcagg gcgtccacct gcgggcgcgg gctcacgccg ccgaagatca
      781 ggagtgtgcg ctgctgcagg tgacggccga aggcctcgaa ggattcgccg acctgagccg
      841 cgagttcacg cgtgggcacg agcacgagca gccgcggcgc gcgatggccg cgcggcacag
      901 ggttctccgc gtcgagtcgc tgcagcagag gcagcgcgaa tgccgccgtc ttgccggtgc
      961 cggtctgggc cgcggccagc aggtcgcgcc cgtcgtgcac ggccgggatg gcgcgctgct
     1021 ggatgggggt gggttcggtg tagccgagtt cctggacggc gcgcagcagc gcggccgaca
     1081 ggccgagttg gtcgaaggac atgtctgatg tttcactcac gcttcccccg cacgcacacg
     1141 catgccggga ccggacgaag cgtcactggt tcaaatgatc gggggcgcga aatgcagacc
     1201 acgatgagtc tgcaggaggg ccgctgggct cgaaagccgg tgatcctcgg ggcgcagacc
     1261 ggactgcgcg gcggcaaact tggggcgcat catgccagaa agcgccgggc cgcgtacgaa
     1321 aatctcttcc gcgctcgttt ggccctcagt tgcgcgagta gatggtcttg ccttccttga
     1381 tcgtctcgac gacccggatg tcgcggatcc tgtccacggg tgtcctgagc ggatcgccgc
     1441 tgacgatcac gaaatccgcg agcttgccgg gcgcgatcga gccgcgacgg gtctcctcgc
     1501 ggtactccca ggccggattg atggtcagcg cctgcagtcc acggtaggga tcgatgcgct
     1561 ggtccggtcc gagcacgtag ccggaacgcg tcgtgcgcgc catcgaggtc cacagcatga
     1621 agaacgggcc gagcggcgtg acgttgaagt cgctgtgatt cgtggccttg agccccgcgt
     1681 ccatcgcgga cctcatcggg ctgatgaacg cggcccgcgt ctcgccgatg ttcctgcggt
     1741 gtacgtcgcc ccagaagaag gtgtggttcg tgaaatagga cggggtcagt cccagcgcga
     1801 cgtagcgcgg gatgtcctcc ggatgctgca tctgcgagtg gatgacgatg gtgcggctgt
     1861 cgtcacccgc cttgaggccc gccgcctccg cagcctggat gacctgctgg atcgcgccgt
     1921 cgccgttcgc atgcacgaag acctgcagcc ccgccttgtg accggcatcg aagatcttcg
     1981 cgaactgctc gtaaggcagc gtggtgttgc cgcgccagtc cggctggccc tggggaccgc
     2041 cggtgagcag cggctcgatg taatacgccg tcttgccctg gacggagccg tcctgcgtga
     2101 tcttgagtcc ctgcagcttg agtcggccat tccactggcc gaaggggaac tccggcttcc
     2161 cgagccactg gtccatctcc gaaaaggctg gtagcacggc gatgtcgatg aacaaccggc
     2221 cctgcgccgc ggcctcctgc aggaggcgga tgtccctggt gtgcgtgaag ccgtcgttcg
     2281 cgagcgtgta gccgttctgc gcgtacatca tctggccggg cttcagcagc tcgagcattt
     2341 cggcttcgct gggctgcggc aacttgttga agaccggcag gaaggccgtt tccatgagga
     2401 gtccttccgg ctccttgccg tcagaccccc gcgcgatgat tccgccgtct ggcgtcggcg
     2461 tgtcgcgcgt gatgcccgcc cattccaggg cttgtgagtt gagcacggcg ccgtgcagcg
     2521 agacgtgcag cacgagcacc ttgtggttcg ggaacgccgc gtcgagctcg cgccgggtga
     2581 gcttgccgcc ctgcaccatc gagtcgtcgt agccccaggc catgatccag ccgccgtccg
     2641 ggaccttcgc cttgtcctgc cacgccttga gcgcggagac gatcgatgcc acggtgtctc
     2701 ccgggccgac cggcgacggc gacacgttga ccatcctgga gagtgctggc gcattgatga
     2761 aatgactgtg cgcatcgacg aagccgggca gcatggtcct gccgtggagt tccttgaccg
     2821 cggtggctgc gcccttgcgc cgcatggctt cggcttcggt gccggcgaag acgatcttgc
     2881 cagcggagac cgctaccgct tcaacatagg tgggcgcggc tccctccatc gtgaggatgg
     2941 ggccaccgcg gatgatgagg tcagccgatc gcgcctgcac cgactgtgct gccgggccgc
     3001 ccgacactgg cgccgacgca ctgctccgct ctgggcccgc agtgatcacg acgacacagg
     3061 tcacgctcgc caccagccat tcgcgcaatt tcgtcattat tcgaccttcc ccttcgtacg
     3121 cccgatccaa tccgccgccg gagcggcctg acccgtccat gaaactcacg gcggcgtcac
     3181 gccattccgc gcgagaaacc ggaacatcgt ctcgtagagg tgcttgctga cgatcggccc
     3241 cgagatgcga tgcgtgtacc ccgggtagag catcatctcg aacggcacag cctccccctg
     3301 cagcttggcg atgatcgcac tcgagtgctc gaagacgacg ttgtcatcgg ccatgccgtg
     3361 gatgatcagg agcgggtcgc tgatcttcgc ggtgtccgcc aaggcgtccg acgtcgcgta
     3421 gaccgcgggc tcggtcttcg ggcggccgag gtatcgttcc gtgtagtgcg tgtcgtagag
     3481 ctcccaccgg gtcaccggcg cgacggcgat gcctgcggcg tagacgccag ggttcgcctg
     3541 cagcatcttg atcgtcatgt acccgccgta tgaccagccg aagatcgcga ccttggccgg
     3601 atcgacgaac ggcagtgtct tgaggtagtt cgctcccgcc agctggtcca cgacctcgac
     3661 ggagcccatc gcgcggaaca gcgtcgattc gaaggcgacg ccacgattgg cggaaccgcg
     3721 gttgtcgagc cggaaataaa tgtagccctg gtcgacgatc gcctgcggca gggcgccgct
     3781 ccaggcacgc gcgacttgct ggctggtcgg gccgccgtaa tgatcgtaga acaccgggta
     3841 gcgcttcccg ggctcgagcg gcggagtgat catcatccag tgcagtggcg tgccgtccgc
     3901 cgccggaatc gtgccgaacg aagtttcgcg gtgactggcg agatacgggg cgtacgggtg
     3961 cgaggggtcg aggcgattct cctcgatcca gtcgaggcgc ttcccggtgc cgtcggcgat
     4021 gtagctctgg ggcggatgat cgggcgagga acgagtgacg acgagcgtct tgcccgcctc
     4081 gtccatggtc gccctggtcc accagccggg ctcggtgagc cgctcgatcc tgtcgggtgt
     4141 cttcaggtcc agcacgtaca cctgctgcgc gaggacgtcg tccttgttgg cggtgaagta
     4201 gacgcgaccc gagtcctcgt cgacgcccgc gagtcccgtc accacccacg gaccgcgagt
     4261 cagctgccgc cactggccgt ccctgaactg gtagagatga ccgtagccgt cgcgctccga
     4321 ccaccagacg aggctgccgt ccttgaggaa cttgtagctg tccgagaggt tgatccagct
     4381 ccgcgccgct gcccgttcct ggaacagcac gcgcgagttg ccggtcgcgg gatcgactgc
     4441 gagcacgtcg agccgggtct gggcacgatt ctgtcgctgg acgtacagcg tcctgccgtc
     4501 gggcgaccag tcgacgcggg cgagatagat gtccttgtcc gcaccgaggt cgacttcgac
     4561 ccgatgcgaa ccgtcggggt ccatcacgaa gagctggacc accgcgttcg gcgtcccggc
     4621 tgcgggatag cgctgctcga aggtcctggt gtcctccgct ccgatagcgg tgcgtgtgac
     4681 gacgccgacc ggcacctcgt cgtagcgctc gaccgcgacc cgggaatcgt cgggcgacca
     4741 ccagtagccg gtcgtgcggt ccatttcctc ctgggcgacg aattcggcct caccccagtg
     4801 gacggtgtcg gacgcctcca cgggcgtgat cggctgcgcc ggtgcggcac gcacgggacc
     4861 cgtccacagc cggccgtccc tcacgaagga aacgtatcgg cccttcgggg agagcgccgg
     4921 gttgagttcg tcctcgctgg tgtccgtcaa ccgggtgacc ttcccgtcga gaccggcgag
     4981 aaacaggtcg ccatccagcg gaacgagaat cgactggccg tcgtccgacc agtcgtaggc
     5041 gacgatgccc ttcaggtcgc cgatgcgctg ccgctcacgc tgcatcttcc cggcctccga
     5101 aagctcccga cccgactcga ggcgagcgga gtcgaccagc atggaccact gcccggtctg
     5161 gcggtcgtag ccccagaggt cataccgttc gcgatcctcg ggatggttct tcagcagcgt
     5221 gatccagcgc ccgtcgggcg agagcttcac ctctcgcggc ctggggccgt caaggctggg
     5281 actggcgaag actcgttcga gggtgagcgc cggggcgggc ctttccggtt ccgtcgcggg
     5341 ggcctcgccg gccagtgcgg ccgccgtcag ggcgcacccg agcattgcgg caagcagtag
     5401 gcgccctggt cgaacgggct tggatcggcg cccggcacgg gcatgaacgt tctgcaaccg
     5461 ggatccctcc tgtgcgctcc ggcgactagg ctcgcacaga cgcacggccg attgccacgc
     5521 cggctggccg cgcgaactac gccagcgccc ggccgtcaca aatctgtaac gtccccgggc
     5581 acatcatgtc gcggtcccca ggatcgtaaa cttcgaggag ggccgagtcg tgctccgacg
     5641 gatgaatcac aggtttcttg cggtctttgc gctcgctgca gccgcgctgc tgggccgccc
     5701 ggcgttcgcc gccgacattt caggcgcggg tgccacgttt ccctatccca tctacgccaa
     5761 gtgggccgag gcctacaaga aggagacggg gatcggcctg aactaccagt cgatcggcag
     5821 cggcggtggc atcaagcaga tcaaggccgg caccgtcacg ttcggcgcct ccgacaagcc
     5881 gctcgagatg aaggaactga ccgactccgg cctggtgcag ttccccatga tcatcggtgg
     5941 cgtggttccg gttgcgaacg tcccgggcct gaagccgggt gccctgaagc tcgacgggcc
     6001 gacgctggcc agcatgtacc tcggtgaaat cacgaagtgg gacgacgctc gcatcaaggc
     6061 gctgaatccc ggcctgagcc tgccgagcct cgcgatcgct cccgtgtacc gctcggacgg
     6121 ctcgggaacc aatttcctgt tcacgtacta cctctcgggc gtcagcccgg ggttcaagtc
     6181 caaggtcggg gccaacacct ccgtcgagtg gccggtcggc atcggcgcga agggcaacga
     6241 gggcgtcgcc aacatgacga cgcagacctc gggagccatc ggctacgtcg agtacgcgtt
     6301 cgccaagcag aacaagatga cgtacatcgt catgaagaac cgtgacggcc ggttcgtcag
     6361 ccccgaagcc aaggccttcc aggcggccgc cgcgaatgcc gactggatga aggcgccggg
     6421 ctactacctg atcctgaccg accagcccgg tgccgcaagc tggccgatca cgggcgccag
     6481 cttcatcctg atgtaccgca atccgaagga cgccaatgcg tcccgcacgg cgctcgagtt
     6541 cttcgcgtgg gcctacgcca agggtggcga cctcgcggcc cagctcgact acgttccgat
     6601 gccgcccggc gtcgtgaagc tcgtcgagtc cacctggcag acgaagatca cctccggagg
     6661 ccagccggtg tggtcgccga agcgctgagc atccagtcgc tgctgcgtcg cgaaaggcgc
     6721 gtcgaacgcg cctttcgcgg cctggccacg tcgagcgcga tcctcgtcct cgcactcctg
     6781 agcggagtga tcatcgcgct cgcctacgac gcctggccgg cgctgcacga gttcggctgg
     6841 tccttcgtca ccgagacgcg ctggaatccc gtcaccgaga agttcggcgc gctcgcaccg
     6901 ctgtacggga ccgtcgtgac ctcgatcatc gcgatggccc tggcggtgcc ggtcagcttc
     6961 ggcatcgcga tcttcctcac cgagacctgc ccggtctggc tgcgcgcccc gatcgggacc
     7021 gcgatcgagc tgctggcatc catccccagc atcgtcttcg gcgtctgggg cctgttcgtg
     7081 ctcgcgccgg tcctgcagca caccatccag ccctggctca tcgatcactt cgggaatctt
     7141 cccgtcatcg gatttctctt ccagggaccg ccgttcggca tcggattgct gacggcggga
     7201 ctcgtgctcg ccatcatggt gctgccgttc atcagctcgg tgatgcgcga ggtgttctcc
     7261 atcaccccgg tcgccctcaa ggaagccgcc tacggcctcg gtgcgacgca gtgggaggtg
     7321 gtccgcggcg tcgtcatacc gtggtcacgc ccgggcatca tcggcggact catgctcggc
     7381 ctcgggcggg cgctcggcga gaccatggcc gtcacgttcg tcatcggcaa cgcgcaccgc
     7441 attaccggct cgctgctggc gccgggcacc acgatttccg ccgccatcgc caacgagttc
     7501 accgaagccg tcggcgacat gtacacctcc gcgctcatcg cgctcggact gttgctgttc
     7561 gtgatcacgt tcatcgtcat cgcggcggcc cggctgctgc tcatgcggct cgaggcaagc
     7621 tcgggggcac gcgaatgagc gcgcagcgtc agcagggcac cgccagcgcc ttcggcaccc
     7681 gcctgcaggt gcgccgccgg gtcgtgaacg tcatcgggtt cgcgctgtcc ggagtggcga
     7741 cattgatcgg tctcgccgtg ctcgcggcgc tgctgtggac actggtctcg cggggcgtgc
     7801 agagcatgtc gcccgatttg ttccacctca gcacgccggc gcccggcagc gagggcggac
     7861 tcgccaacgc catcttcggc agtgtcgtca tgacgatgct gggcatcgcg atcgggggcc
     7921 cggtcggcct gctggcgggc acctatctgt cggagtacgg ccgcggcacg gtgctggcct
     7981 ccgtcattcg attcatcaac gacgtcctgc tcagcgcgcc gtcgatcgtc atcggcctgt
     8041 tcgtctacac cgtcgtggtc atgaacgccg gccacttctc cgcgctcgcc ggcgcgctgt
     8101 cgctcgcgat catcgccctc cccgtgatgg tccggaccag cgaggacatg ctgcagctcg
     8161 tgcccaactc gctgcgcgag gcttcggcgg cgatgggcgc gaatccgtgg cgcacgatcc
     8221 tcgccaccat ctatcccgcc gcgcgtaacg gcctgctgac gggcctgctg ctcgccgtcg
     8281 cccgcatcag cggcgagacg gcgccgctgc tgttcacggc gctgaacaac cagttctgga
     8341 gcgcggacct caacaagccg atggcgaacc tgccggtcgt catcttccag ttcgcgatga
     8401 gtccgtacga ggactggcag ggcctcgcct gggcaggcgc actgctcatc acctcgttcg
     8461 tgctcgtcct gagcatcggc gcgcgcctcc tcgccgcaac ggcccgtacc cggaagtcgg
     8521 gctgaccctg ctccccgccc ggccggcgtc atgccgcctc ttccaatcgg cctcgcggaa
     8581 gaatagattg cactatattt cgatgtttct cgaattatga aagagccgat cgttctcaag
     8641 gccctggcgg ccctggccca ggtcaaccgg ctgcgggtgt ttcgggcgct ggtcgtcgcc
     8701 ggccaggccg gtgcgacgcc cggcgcgatc ggcaggcggc tcaaggtatc gccggcgacc
     8761 ctctcgttcc acctcaagga actggtcaac gccggcctgg tcacccagga acgcagcggc
     8821 cgcaacctga tctaccgggc ggactacgag gccatgaacg ccctgctgtc ctacatgacg
     8881 gagaactgct gccagggcat ggcatgcgac gtgcctccgg aacgcatccg cgctcgctga
     8941 ggaccgtgca tgaccacgaa cgtcctgatc ctgtgcaccc acaactccgc gcgcagcgtc
     9001 ctggcggagg ccatgctcaa tcaccacgcc gcacgtctcg ggctcgacgt ccggggatac
     9061 agcgcgggca gcgcacccag cgggcgtgtc aatccgctcg ccctgtcggt gctcgaggac
     9121 gccggggtgt ccacggccgg cgcccgcagc aagagctggg acgagtttgc acggagcgac
     9181 gcgccgcaca tgcgcgtcgt gatcacggtc tgcgacagcg cggcgggcga ggcatgcccc
     9241 gtctggccgg gcagccccat ccgggttcac tggggctatg ccgacccatc gaatgccgca
     9301 ggtgacgcgg cggcgcgcag ggtggcgttc gacctgacgc gacaggccat cggctaccgg
     9361 atgctgcagt tgctcgcgct cccgctcgcg tcaatggaag acgccaggtt gcggcaggca
     9421 ttgctcgaga tcgcgcggtc ctgaggttgc actatggcga cggtgtcacg gcgtctctcc
     9481 ttcctcgatc gctacctgac ggtctggatc ttcgctgcga tggcggtcgg catcgcgatc
     9541 ggatggctgg ccccgggcgt cgaggcattc gtggaccggt acaccgtcgg caccaccaat
     9601 gtgccgatag cgctcggcct gatcctgatg atgtacccgc cgttcgcgaa ggtgcgttat
     9661 gaggacctgc ccgacgtgtt ccgcgacaag aaggtgctgg ctctctcgct ggtgcagaac
     9721 tggctggtag gaccggtgct catgttcctg ctcgccatca ccttcctcgc ggacaaaccc
     9781 gaatacatgg cgggcctgat cctgatcgga ctggcgcgct gcatcgccat ggtgatcgtg
     9841 tggaacgaac tcgccgacgg ctcgaccgag tacgccgcgg ggctcgtcgc attcaattcg
     9901 atcttccagg tgctgttcta cagcgtgtac gcctgggtgt tcgtgacggt gctgccgccg
     9961 ctgttcgggc tgcacggctc ggtcgtggac atctcgatcg gacagatcgc gcggagcgtg
    10021 ttcgtctacc tcggcatccc gtgcatcgcg ggcgtcctga cccgggtcgt gatgctgcgc
    10081 ttcgtgagca aggactggta ccacggccat ttcgtgccgc tgatcagccc gatcacgctc
    10141 gcggcgctgc tcttcacgat cgtcgtgatg ttcagcctca agggcaagct gatcgtcacg
    10201 attccgatgg acgtcgtgcg aatcgccatc ccgctgctca tctacttcgt ggtcatgttc
    10261 ctcgtgtcgt tcgccatggg tcgccgcgcg ggcgcggcct acccgaagtg cgcgaccttg
    10321 tccttcactg ccgcgagcaa caatttcgag ctggcgatcg ccgtggcggt cgcggtgtac
    10381 ggcatcgatt caggcgcggc gttcgccgcg gtcatcggcc cgctcgtcga ggtgccggtc
    10441 atgatcgcgc tcgtgaacgt cgcactgcgg tttcagcaac gctggttcgg tggcccgccg
    10501 gccgccggcg cgccaactcg ggcctgacgg ccctgggaga caccatgagc atcgacatca
    10561 agcaggtcgt gaaagagagg tacggagaag ccgcccgcaa ggtcatctcc gtcaaggcgg
    10621 aagcgtcgtg ctgcggtccg gcgccgtcat gctgcgaccc gatcacgtcc aatctatacg
    10681 acgccgcgca gaccagggac ctgcccgagg ccgcggtgct cgcttccctc gggtgcggca
    10741 acccgacggc gctcgccgag ttgcaggccg gcgaggtcgt gctggacctc ggctcgggcg
    10801 gcggcatcga cgtgctgctg tctgcaaagc gcgtcgggcc caccggcaag gcgtacggcc
    10861 tcgacatgac cgacgacatg ctggcgctcg cgaacgagaa caagcgcaag gccggggcga
    10921 ccaacgtcgg gttcctgaag ggcgagatcg agcacatccc gctgccggac gcctcggtcg
    10981 acgtgatcat ctcgaactgc gtcatcaacc tctcggcgga caaggatcag gtcctccgtg
    11041 aagcgttccg cgtgctgcgc ccgggtggcc ggttcgccgt gtccgacgtc gtcacacgcg
    11101 gcgaaatcga gccgcagatc cgcgccagcg tcctcgcctg ggtcggatgc gtggcgggcg
    11161 cactcgacga gcaggtctat cgcgacaagc tcgcggccgc cggctttacc gcgatcgacg
    11221 tcgagccgac gcgggtctat cgcgtggacg atgcgaaaga cttcatcgcc gcgagcggtc
    11281 tcgatgcagc cgcgctggcg cccaaggtcg acggcaagtt catgagcgcg ttcgtgcgtg
    11341 cgcgcaagcc gctcgcctga acggtcctgg ctggatcgaa ttgacatgag cgaacccgcc
    11401 tacctgccgt tcctccagca gacgctgagg tatttcgacc ggccgcacga cggtccggcg
    11461 ctgacgcccg tctcgtctcc cgccgcgtgg cggaggggcg acctggcaga tgcgcgatgg
    11521 cgcgtacgac tcaccgacga cgaggtggcc gagctgcgac gggccgttgc cgcgtggctc
    11581 gcacgctcgc tgccgctcgc cgcgatgacc aagtccgact ttccgctgcc gaccctggcc
    11641 gcccggattc gcgactggcg cgacgaggtt gccaatggac tcggcctcca ggtcatctcc
    11701 cgcgtgccgg tcgaggcgtg gtcgctcgag gaggcctcgg cattcttctg gggcctcgga
    11761 cagcaccttg ggcgacctgg cgcccagaac cgcaagggcg atctcctcgg gcaggtgcgc
    11821 gataccggcg ccgaccgcac cgaccgctac gtgcgccagt acatgacgac ccaggacatc
    11881 ccgttccact gcgacgccgc ggacatggtc ggactgttgt gcctgcggac ggcgaagtcc
    11941 ggtgggctca gtcgcatcgc ctcctcggtc agcgtgtaca acgaactgct gaggcgacga
    12001 cccgacctgg cgtcgaggct gttcgagccg ttcatgctcg acacccgcga cgaggccgag
    12061 gcgaatggtg tcaaggcatt gccgataccg ccgtgccgat acggtggcgg cgtgctgcgg
    12121 acattctggc actcggagta tttccgcacg gcgcagcgcc acgagggcat ccttctgacg
    12181 gacgccgagc acgagctgct cgacctgtac gacgagatct ccgcatctcc cgagttccac
    12241 atcgacatgg cactcgagcc cggcgacgtg caactgctgt cgaaccacac ggtcatccac
    12301 gcccgtaccg actacgagga ttacccggag gaggaccgca agcggcacct gctccggctg
    12361 tggatcacgc tttaggcagg cactggcgag tcgggagaat cgagcgggca ctgccgtgcg
    12421 cggtcgatcg cgaagcgcag cgcctggtag tcctgtgtag ccatgtgcgt ctccgctcga
    12481 acgtcagggt atgcccgccc ccggcacctc gacctcgacc acccgcaggg tcgcgctcgt
    12541 ttatgtcttc agggctacgt cttcgtgtcc atcgggaatt gcggccaggt gtcgatggct
    12601 cacgcgccgg aacgattgat gagcgcaacg tgcaggtcgg tgatcgcccg cgtgatgtgg
    12661 cccttgcctg tcacgtcgtc gaagaacatc acatcgccag gaaccaggcg ccgggtggtg
    12721 ccgtcgctgg ccacgacttc gccctcgccg gagaggacga tgcagatgta gggctgcggg
    12781 gccgtgtgcc atgtagcccc ggccccggcg ggcgcggagt acgtcaccag ggtgctgacc
    12841 atgatcggat ccgtcatgcg cccgacggga ttcggcggcg gcccgagcgc cgcgtcatgc
    12901 ctcggtagct cgcggacgcc gatgtgcgtc tcgcccagtt cgtcggtgtg cacgcgtggg
    12961 aatgtgatca tcgggttctc cgcgggcagg gtccggaata ccggtggcaa ccccgggccg
    13021 ccggctgggc aaatgcaata cttcgccagg acggcgggcg cagctagcga atttccgcag
    13081 aatcgcgcgg tctactcgct ctgcagccgc ttaaagcctt cgatgatcgg atgccatcct
    13141 gcgaccagcg agatcatgat cacgtccatc tgcaggtcgt ccggcgcctg gtcactggcc
    13201 gtcgacttgt agctgaaggt gagcccgatg ttgtcgttga tctggtaacc gagcgtgaaa
    13261 ccaaagccgt agttgttcag ctttgatcct tcgacgccat cgatggtggt cttaccgccg
    13321 ttgtaataga ccgcatcgag cgacccccac aggctcgcgg tgaagtcacg cgtcaggtga
    13381 gcgtcgatct gatacagagg atcggcctcc agggtctgcc cgaggtagtc cgtgttgtcg
    13441 ccaaagagcc aaaccgcggg caggaattcc aaggtcgtgc gccgggtcgg cacccattcg
    13501 ccgatctgcc agacgatggg aaagccgatg cggccatacc agcggttctg gccgatgttc
    13561 aacggctggc tgctgtcgta ctcgccgatc gggatcgcga gatccgccag cacgtcgagc
    13621 gaaaagcccg gttcgaagcg gctgacgtct tgaaggtttt cctgcgcctt ggggccgatc
    13681 acgttgaggg tgaattcaac catcgggtcg ccgaagccgc tcaccgactg cgagaccggg
    13741 gtgccacccg gcgcgatgac gtcacccgag attcgtccca tgggcagcag caccgccccc
    13801 atggccgacc gatcgaacaa cgtgaaggtg cgcgcatacc cggccaggat gagcgtactt
    13861 tcgaagtcag cgcccggcac gacggtgagc gcggggtcga acggattcgt attgccgctc
    13921 atggacgtca cgatcagcgg cactgcattc gcgccggaca gggtgtgcca gta
//
LOCUS       DAOMFD010000041        13870 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1035_length_13870_cov_32.795802, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000041 DAOMFD010000000
VERSION     DAOMFD010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13870
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1035_length_13870_cov_32.795802"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..391
                     /locus_tag="PL152_04010"
     CDS             <1..391
                     /locus_tag="PL152_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004998455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HQR48474.1"
                     /translation="VCEDYIRRRAVRHLEKGRVCIFAAGTGNPFFTTDTAASLRAIEI
                     EADLLLKATKVDGIYDSDPVVNPAARRYDRLKYDQVLDGRLNVMDATAIVLCRDHDLP
                     LRVFNLNEAGALVRVARGEEVGTLVTN"
     gene            425..982
                     /gene="frr"
                     /locus_tag="PL152_04015"
     CDS             425..982
                     /gene="frr"
                     /locus_tag="PL152_04015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HQR48475.1"
                     /translation="MLEDIKKDAADRMAKCVAALRNELKKLRTGRANTALLEQVRVEY
                     YGSEVPLQQVANVAVEDARTLTVVPWEKQLVPVIEKAIMKSELGLNPATAGSVIRVPL
                     PALTEERRRELTKVVRHEGENARVAIRNVRRDVLQELKEMLKEKLISEDVERKAHEEV
                     QKLTDKYVAEVDHVLADKEKDLMQV"
     gene            985..1728
                     /gene="uppS"
                     /locus_tag="PL152_04020"
     CDS             985..1728
                     /gene="uppS"
                     /locus_tag="PL152_04020"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010930350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HQR48476.1"
                     /translation="MAQTHTREPGTPRHVAVIMDGNGRWAARRALPRHMGHRAGVKAV
                     RAIVEGCARRGVEALTLFAFSSENWGRPADEVSRLMELFVEAMDREVDELHRNGIRLR
                     FVGDLGKLKPHVRDGVAAAESRTAGNARMTVYVAVSYGGRWDIVRAARHLARRVAAGE
                     MAAEAIDERGFASQLVLGEAPDPDLFIRTGGEQRISNFLLWNLAYTELYFCDCLWPDF
                     DDGELARAFRHFAGRQRRYGLTADQVAGG"
     gene            1721..2533
                     /locus_tag="PL152_04025"
     CDS             1721..2533
                     /locus_tag="PL152_04025"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HQR48477.1"
                     /translation="MASGLRQRIATALVLAAVVITVLVAMPPVVAVAVVAVVIVAGAW
                     EWAGFAGLGGSAARWFYAAAVGVAVLVAWRWTGEPAALAVFLRGAALWWAAAFAWVLL
                     APGKGGRVAAAVAGFAVLVPAAIGLARLAMLEPHGQLQLLFLIVLIAAADIGAYFGGR
                     SLGRHKLAPTVSPGKTWEGFAAGMVGAAIAAACGAVAFGSDVAGWIAMCVVVALVSVV
                     GDLVESMFKRHAGLKDSSGLLPGHGGVLDRIDSLTAAGPAFLLGLQVLGMAA"
     gene            2530..3720
                     /gene="ispC"
                     /locus_tag="PL152_04030"
     CDS             2530..3720
                     /gene="ispC"
                     /locus_tag="PL152_04030"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HQR48478.1"
                     /translation="MMRVAVLGSTGSIGASTLDVLGRHRDRYAVVALAASSSHARLLE
                     QCRVHRPRVAVLQDPRAARELAAALRAEAIPTRVECGEDALSDIAGAADVDAVMAAIV
                     GAAGLQSTLAAANAGKRVLLANKESLVVGGRLLIEAARRAGASLIPIDSEHNAIFQCL
                     PAGLQAGLPARGVRRILLTASGGPFLRTSRADMERVTPEQACAHPRWKMGRKISVDSA
                     TLMNKGLELIEACLLFGMEPRQVEVVVHPQSIVHSMVEYVDGSILAQLGNPDMRTPIA
                     HALGWPERIQSGVESLDVVAAARLDFEAPDVERFPALRLAREAAESGGTSPAVLNAAN
                     EVAVAAFLAGRLGFTGIPGVIDAVLGQHSSEAASSLETVLGADAWARERAESLLVGAA
                     RSRA"
     gene            3717..>4171
                     /gene="rseP"
                     /locus_tag="PL152_04035"
     CDS             3717..>4171
                     /gene="rseP"
                     /locus_tag="PL152_04035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQR48479.1"
                     /translation="MTILSYVLAFLVAIGILVAVHEYGHFWMARRVGVRVIRFSIGFG
                     KVLWKRTDRHGTEFAISAIPLGGYVKLLDEREGPVDESQLASAYNRKPVWQRILVLLA
                     GPVANFLFAVVAYWVLFVAGIPALKPVIGEVKPDSVAARAGLASGQEVVA"
     assembly_gap    4172..4271
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4272..5233
                     /gene="rseP"
                     /locus_tag="PL152_04040"
     CDS             <4272..5233
                     /gene="rseP"
                     /locus_tag="PL152_04040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HQR48480.1"
                     /translation="PDSVAARAGLASGQEVVAVGERPTATREAVVLAILDQLMAGGPI
                     DLQVRTVDGAERVAPLRIEGSPLPLTEPGALLPGLGFEFWFPAIPASVGKVLPGSPAE
                     RAGLQPGDRVVAVGSQPVADFPALVKIVQPQPGRTLTFTLERAGNRVVVPVEVDAQRE
                     DGKLIGRIGVQPAASQPVPEQMRTRERYGPLVAFGRAVEKTWDMSALTVRLLWNVAAG
                     DVSARNLSGPINIAEYAGFSARQGILAFLSFLSVVSVSLFVLNLLPIPILDGGQIVYQ
                     LAELAKGRPLSERAQALGQQIGIALLLALMSFAFYNDISRLFS"
     gene            5244..7652
                     /gene="bamA"
                     /locus_tag="PL152_04045"
     CDS             5244..7652
                     /gene="bamA"
                     /locus_tag="PL152_04045"
                     /inference="COORDINATES: protein motif:HMM:TIGR03303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HQR48481.1"
                     /translation="MRLKYMAALPALLAVPAVFAQATMGDFTVGDIKIEGLQRISEGT
                     VFNYLPVNIGDRMDAQRVQEALRALYATGFFKDVELRRDGGTLIVVVKERPSIESFTI
                     TGNKDIKTEDLEKSLRNVGLARGKVFNQSTLDEVDRFLTDQYYSRGKYGVVVDTRVTD
                     LPNNQVSIAIDVKEGKRSRIRQINIVGNEAFSEDQLREVFKLRTPNWLSWYRDDDKYS
                     REELSGDIESLKSFYLDRGYADFDVETSQVAIAAEKDDIFITLNVHEGDVYRVSDVKI
                     AGDLVVPEEDLMSLVQVRRGDIYSRKFITATTELMALRLGQDGYAFAKIDPVPQENPK
                     TKEISITFLVQPGSRAYVRHINFEGATGTNDDVFRREMRQMEGGYLSNAAVERSKQRL
                     QRLPFIEKVDVETNPVPGTPDLVDVDFKIKEGMPGQFSGGIGYSQSQSFMLNASVVHS
                     NFLGTGNRVAAEVNAGQYSQVYSLSHTDPYVTVDGVSRTLSASYRNYKQYTSSSSQFD
                     TTTGALAVNYGWPLSEFQSVATGLSAQHSDLFTDPNQSAQQSLLWVQQNGNPYTEKST
                     AGMPPTTFTYYGTKFDTFVLTAGWSYDSRNRSIFADRGARHIVNLSYTVPGSDVEFYS
                     LTYDYLQFVPLTRWFTLMNNFSTGYAAALGSTTSIPPYRNFFAGGAESVRGYKASTLG
                     PKDTFGNPYGGNIKLTNQFELLLPVPEKVKGSARASLFFDAGYVFSSEDVKFYGPNNV
                     TPEKYDFAFDMLRYSTGVAVQWMAPMGIFRFSYAIPLNARKGSPTVYGDETENFQFSI
                     GQAF"
     gene            7666..8196
                     /locus_tag="PL152_04050"
     CDS             7666..8196
                     /locus_tag="PL152_04050"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HQR48482.1"
                     /translation="MRIKTATLSLLVLAASALPAGGALADTKVGVVNVSRLLQESPQA
                     QAASQALESEFAGRRRELEAQQKDLKAKEDKLQKDGAVMAANERTNAEKTLRDGQREL
                     ARKQNEFMEDLNVRRNEALGQLQRTVLQEVQAYAKTAGLDVVVADALYASPAVDITNQ
                     VLAALQARKGAAPAKP"
     gene            8222..9262
                     /gene="lpxD"
                     /locus_tag="PL152_04055"
     CDS             8222..9262
                     /gene="lpxD"
                     /locus_tag="PL152_04055"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HQR48483.1"
                     /translation="MPARLAELAVRFGCELRGDPDAVVERVASLQDAAPGSLTFLANP
                     HYRRHLAGTRATAVVLDADSAAGCPVAALVSRNPYASYARMAQVLHPPPAVTGGRHAS
                     AVVDEGAEVDPSAWIGPGAYVARGARIGARASIGPGSIVLDGARVGDDTRLVARVTLC
                     AGAQVGRRCLLHPGSVIGGDGFGHAPDADGYVRVPQVGSVVVGDDVDVGCNSTIDRGA
                     IGDTVIGNGVKIDNLVQVAHNVRIGEHTVLAAYVGISGSATIGRRCMLAGMVGVAGHL
                     EICDDVVVTGRTTVAGSIRKAGVYSGSLTADDARRFRRNAARFNQLDDLARRLRRLEG
                     AVEPDDGGGTDE"
     gene            9255..9701
                     /gene="fabZ"
                     /locus_tag="PL152_04060"
     CDS             9255..9701
                     /gene="fabZ"
                     /locus_tag="PL152_04060"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003377815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HQR48484.1"
                     /translation="MSDQGGFGIDEVMRRLPHRYPMLLVDRVLECVPGERILALKNVT
                     MNEEFFSGHFPGRPVMPGVLILEAMAQAAGILTFVTAGVYPDDSVRFYFAGIDKARFR
                     RPVVPGDQVMLSATLERRIRTIWKYSTLATVDGQEVASAEMMVMPG"
     gene            9731..10510
                     /gene="lpxA"
                     /locus_tag="PL152_04065"
     CDS             9731..10510
                     /gene="lpxA"
                     /locus_tag="PL152_04065"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004751935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HQR48485.1"
                     /translation="MSGAIHPTALVAADAELAEDVRVGPFAVIGAGVRIGRGTVVGPH
                     AVIEGPTVIGEGNRIFQFASVGADPQDKKYRGEVTRLEIGDRNVIRECATIHRGTVQD
                     QGVTRIGNDNLFMAYSHVAHDCVIGSQCVLANQATLAGHVHLGDWVIMGGLSAIHQFG
                     KVGAHAFIANNAAVTLDVPPYVMAVGQPAGAHSVNTEGLRRRGFSSEQIGNIRNAFRL
                     LYRSGLKLAEATDRLAELAAGQPELRPLVEFLPQATRGIVR"
     gene            10523..11695
                     /gene="lpxB"
                     /locus_tag="PL152_04070"
     CDS             10523..11695
                     /gene="lpxB"
                     /locus_tag="PL152_04070"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HQR48486.1"
                     /translation="MIPSVDAATFGLVAGEASGDNLGGPLIQALAARATASRFVGIAG
                     PRMLAAGCEAWHASDELAVMGLAEVLRHLPRLVRLRRDLLERFSAARPDVFVGIDAPE
                     FNLRVAAQLKAQGIATVQYVSPQVWAWRQGRVRTIGEAVDLVLCLLPFEVDFYRAHGV
                     RAEFVGHPLADRVPLVSDRAAARSALGLPAEGSVIAVLPGSRHGEVSRLGAPFAATIA
                     WLATQRPGTRFVAPMASPAARESFQHSLATHAPGVEVVVVDGDAQRCMAASDVVLLAS
                     GTATLEAALVKRPMVVAYRVAPLTNWLLRGLRLVKTEFFSQPNLLAGRRLVPEHFQSE
                     VRPDVLGPSVLAQLERPDLQELTGAFTDMHRALRRDASARAAEAILGLAAERRGAA"
     gene            11692..12321
                     /gene="rnhB"
                     /locus_tag="PL152_04075"
     CDS             11692..12321
                     /gene="rnhB"
                     /locus_tag="PL152_04075"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003098580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HQR48487.1"
                     /translation="MSVRRDPRQAGFDWRRPGLVAGVDEAGRGPLAGPVVAAAVILDA
                     SNPVDGLADSKVLAPSRREELDALIRERCVAWGLGWADAAEIDSLDILQATMLAMRRA
                     LAALAVAPAHVIVDGNRCPDLRGIDLECTIEAVVKGDATVACVSAASVIAKVARDAHM
                     RELDGLYPGYGFAEHKGYPTAAHVAALRRLGPSAVHRRSFAPVQSLDTR"
     gene            12333..>13870
                     /gene="dnaE"
                     /locus_tag="PL152_04080"
     CDS             12333..>13870
                     /gene="dnaE"
                     /locus_tag="PL152_04080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HQR48488.1"
                     /translation="MARFVHLRVHTEYSLVDGVVRVESEHKDGKVVREGLVDACARLG
                     MPAVAVTDQGNLFALVKFYTAAQARGVKPLVGVDALLSEEGERAEPSRLVLLCQDERG
                     YRNLTRLVTRSYLEGQGRHGPTLRRSWLDPASTAGLIALSGGREGDVGRALLAGREED
                     ARSTVASWLELFGDRYYLELQRTGRPGEEECIAGSLALATALGVPVVATNDVRFIRRD
                     DFESHEARVCIHDGTLLADPSRPRRYSEEQYLRSPEEMAKLFADVPEALENSVEIARR
                     LNLELRLGKASLPAYPVPAGQTTEEYLRAEARSGLESRLSAAAAAPGLAPAIERAPYE
                     ARLATELDVICQMGFAGYFLIVADFIRWARDNGVPVGPGRGSGAGSLVAWVLGITDLD
                     PIRHDLLFERFLNPERVSMPDFDVDFCMEGRDRVIEYVAERYGRERVSQIITYGTMAA
                     KAVVRDVARVLALSYGFADGIAKLIPFELGITLDDALEKEEELRRRYKSEDEVREVID
                     LARSL"
BASE COUNT         2054 a         4686 c         4971 g         2059 t
ORIGIN      
        1 ggtctgcgag gactacatcc gccgccgcgc ggtgcgccac ctcgagaagg gccgcgtctg
       61 catcttcgcg gccggcacgg gcaacccgtt cttcacgacc gacaccgccg cgagcctgcg
      121 cgccatcgag atcgaggccg acctgctgct caaggccacc aaggtcgacg gcatctacga
      181 ttccgacccg gtggtgaacc cggccgccag gcgctacgac cggctcaagt acgaccaggt
      241 gctcgacggg cgcctgaacg tcatggacgc gacggccatc gtgctctgcc gcgaccacga
      301 cctgccgctg cgcgtgttca acctcaacga ggccggcgcg ctggtgcgcg tcgcgcgcgg
      361 cgaagaggtc ggcacgctcg tcacgaactg aggcggcccg cgccgccgcc gctggagact
      421 gcccatgctc gaggacatca agaaggacgc cgccgaccgc atggcgaagt gcgtcgccgc
      481 gctcaggaac gaactgaaga agctgcgcac cgggcgcgcc aacacggccc tgctcgagca
      541 ggttcgggtc gagtactacg gcagcgaggt gccgctgcag caggtcgcca acgtcgcggt
      601 cgaggacgcg cgcacgctca cggtggtgcc gtgggagaag cagctggtgc cggtgatcga
      661 gaaagccatc atgaagtcgg agctcgggct gaacccggcc acggcaggct cggtgatccg
      721 cgtgccgctg ccggcgctca ccgaggagcg ccgacgcgag ctcaccaagg tcgtgcgcca
      781 cgagggcgag aacgcccgcg tggcgatccg caacgtgcgg cgcgacgtgc tgcaggaact
      841 caaggaaatg ctgaaggaga agctcatctc cgaggacgtg gagcgcaagg cccacgagga
      901 ggtgcagaag ctcaccgaca agtacgtggc ggaggtggac cacgtgctcg ccgacaagga
      961 aaaggacctg atgcaggtct gaccgtggcg cagacgcaca cccgcgagcc gggcacgccg
     1021 cgccacgtcg ccgtcatcat ggatggcaac gggcgctggg cggcgcggcg cgcgctgccg
     1081 cgccacatgg ggcaccgcgc cggcgtcaag gcggtgcgcg cgatcgtcga gggctgcgcc
     1141 cgtcgcgggg tggaggcgct cacgctgttc gcattctcca gcgagaactg gggacgcccc
     1201 gccgacgagg tgtcccgtct catggaactg ttcgtcgagg cgatggaccg ggaggtcgac
     1261 gagttgcacc gcaacggcat ccgcctgcga ttcgtcgggg acctcggcaa gctcaagcca
     1321 cacgtgcgcg acggcgtggc cgcggccgag tcgcgcaccg cgggcaacgc ccgcatgacg
     1381 gtgtacgtcg cggtttccta cggcggccgc tgggacatcg tgcgcgccgc gcgccacctc
     1441 gcccgtcgcg tggcggcagg ggagatggcg gccgaggcca tcgacgagcg gggtttcgcc
     1501 tcgcaactcg tgctcggcga ggcgcccgat cccgatctct tcatccgcac cgggggtgag
     1561 cagcggatca gcaacttcct gctgtggaac ctcgcctaca cggaactcta tttctgcgac
     1621 tgcctgtggc cggatttcga cgacggggaa ctcgcgcgcg cattccgcca tttcgcaggc
     1681 cggcagcggc gctacgggtt gacggcggac caggtggccg gtggctagcg gcctgcgcca
     1741 gcgcatcgcg accgccctcg tgctggcggc cgtggtcatc acggtgctgg tcgcgatgcc
     1801 gccggtcgtc gccgtcgcgg tggtcgccgt ggtcatcgtg gcgggtgcct gggagtgggc
     1861 cggattcgcc gggctcggtg ggagcgcggc acgctggttc tacgccgcgg ccgtgggtgt
     1921 cgccgtgctg gtcgcctggc gctggaccgg cgagccagcc gcgcttgccg tgttcctgcg
     1981 tggcgcggcg ctgtggtggg cagcggcatt tgcctgggtg ctgctggcgc ccggcaaggg
     2041 tggccgcgtg gccgccgccg tcgccgggtt cgcggtgctc gtgccggccg cgatcggact
     2101 cgcgcggctc gccatgctgg agccgcacgg ccagttgcag ctgctgttcc tgatcgtcct
     2161 gatcgcggcg gccgacatcg gtgcctactt cggcggccgc agcctgggtc gacacaagct
     2221 ggcgccgacc gtgagcccgg gcaagacctg ggaaggtttc gccgcgggca tggtcggcgc
     2281 ggcgatcgct gcggcgtgcg gcgcggtggc cttcgggtcg gacgtggccg ggtggatcgc
     2341 gatgtgcgtc gtcgtcgcgc tcgtgtcggt cgtcggcgac ctcgtcgaga gcatgttcaa
     2401 gcgccacgcc ggtctcaagg acagcagcgg actgctgccg gggcacggcg gagtgctcga
     2461 ccggatcgac agcctcaccg cggcgggccc ggccttcctg ctcgggctgc aggtcctggg
     2521 gatggccgca tgatgcgcgt ggccgtcctc ggcagcaccg gcagcatcgg cgcgagcacg
     2581 ctcgacgtgc tcggtcgcca ccgcgatcgc tacgcagtcg tggcgctcgc ggcttcgtcg
     2641 agtcatgcgc gcctgctcga gcagtgccgc gtgcaccggc cgcgcgtggc ggtgctgcag
     2701 gacccgcgcg ccgcgcgcga gcttgcggcg gcgctgcgag cggaggcgat ccccactcgc
     2761 gtcgagtgcg gcgaggacgc gctcagcgac atcgcgggcg cggccgacgt ggacgcggtg
     2821 atggccgcga tcgtgggggc ggcgggtttg cagtcgacgc ttgcggccgc gaatgccggc
     2881 aagcgcgtgc tgctcgcgaa caaggagtcg ctggtcgtcg gtggtcggtt gctgatcgag
     2941 gctgcgcggc gggcaggggc gtccctgatc ccgatcgaca gcgagcacaa cgcgatcttc
     3001 cagtgcctgc ccgcgggtct ccaggcgggg ctgccggcgc gcggtgtccg acgcatcctg
     3061 ctcacggctt cgggcgggcc gttcctgcgc acctcccgcg cggacatgga acgcgtcacg
     3121 ccggagcagg cgtgcgcgca tccccgctgg aagatgggcc gcaagatctc ggtcgattcc
     3181 gcgacgctca tgaacaaggg gctcgagctc atcgaggcct gcctgctgtt cggcatggaa
     3241 ccccggcagg tcgaggtggt cgtacatccg cagagcatcg tgcattcgat ggtggagtac
     3301 gtggacggtt cgatcctcgc ccagctcggc aatcccgaca tgcgcacgcc gatcgcccac
     3361 gcgctcggct ggccggagcg catccagtcc ggtgtagaat cgctggacgt ggtggcggcc
     3421 gcgcgcctcg atttcgaggc cccggacgtc gagcggttcc cggcgctcag gctggcgcgg
     3481 gaggccgctg agtccggcgg cacgagtccg gcggtcctga acgcggcgaa cgaagtggcg
     3541 gtggcagcct tcctggcggg aaggctggga ttcaccggca tcccaggcgt catcgacgcg
     3601 gtgctcgggc agcactcgag cgaggcggca tcgtcgctcg agaccgtcct cggggcagac
     3661 gcgtgggcgc gcgagcgcgc ggagtccctg ctcgtcgggg cggcgaggtc gagggcatga
     3721 cgatactgag ctacgtcctg gcgttcctgg tggcgatcgg catcctggtg gccgtgcacg
     3781 agtacgggca cttctggatg gcgcgccgcg tgggcgtccg cgtcatccgc ttctcgatcg
     3841 gattcggcaa ggtcctctgg aagcgcaccg accgccacgg cacggagttt gccatttctg
     3901 caattccctt aggcggttac gtcaagcttc tcgacgagcg cgaaggtccg gtcgacgagt
     3961 cgcaactcgc gtcggcctac aaccgcaagc cggtctggca gcgcatcctc gtgctgctcg
     4021 ccggaccggt ggccaacttc ctgttcgcag tcgtggcgta ctgggtcctg ttcgtcgcag
     4081 gcatcccggc cctgaagccc gtgatcggcg aggtcaagcc cgattccgtg gcggcgcggg
     4141 ccggactggc atcggggcag gaggtcgtgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4261 nnnnnnnnnn nagcccgatt ccgtggcggc gcgggccgga ctggcatcgg ggcaggaggt
     4321 cgtggcggtc ggcgagcgac ccaccgcgac tcgcgaggcc gtggtactcg ccatcctcga
     4381 ccagttgatg gcgggcgggc cgatcgatct ccaggtgcgc accgtcgacg gcgccgagcg
     4441 cgtcgccccg ctgcggatcg agggcagccc cctgccgctc accgaaccgg gcgcgctgct
     4501 gcccgggctc ggattcgaat tctggttccc cgcgattccg gccagcgtcg gcaaggtcct
     4561 gccggggagc cccgcggaac gggcggggct gcagccaggg gaccgcgtcg tggccgtggg
     4621 ctcccagccg gtggccgact tcccggcgct cgtgaagatc gtccagccgc agccgggtcg
     4681 cacgctcacg ttcaccctgg agcgtgcggg caaccgggtc gtggtcccgg tcgaggtgga
     4741 tgcccagcgc gaggacggca agctgatcgg ccgcatcggc gtgcagccgg cggcgtcgca
     4801 acccgtcccg gagcagatgc ggacgcgcga gcgctacgga ccgttggtcg cgttcggcag
     4861 ggcggtggaa aagacctggg acatgtccgc gctcaccgtg aggctgctgt ggaacgtcgc
     4921 ggccggcgac gtctcggccc gcaacctgag cggcccgatc aacatcgccg agtacgccgg
     4981 tttctccgcg cgacagggca tacttgcgtt cctgtccttc ctgtccgtcg tcagtgtgag
     5041 cctcttcgtg ctgaacctgc tgcccatccc gatcctcgac ggcggccaga tcgtctacca
     5101 gctggccgaa ctcgccaagg gccgcccgct gtcggaacgc gcccaggcgc tgggccagca
     5161 gatcgggatc gcgctgctgc tcgcgctcat gagcttcgcg ttctacaacg acatctcccg
     5221 cttgttcagc tgagccgccc gccatgcgat tgaaatacat ggccgcgctg ccggccctgc
     5281 tcgccgtacc cgccgtgttc gcccaggcca cgatgggcga cttcacggtc ggcgacatca
     5341 agatcgaggg cctgcagcgc atcagcgagg gcacggtctt caactacctg cccgtcaaca
     5401 tcggcgaccg gatggatgcg cagcgcgtcc aggaggcgct gcgcgcgctg tacgcgaccg
     5461 gcttcttcaa ggacgtcgag ctgcgccgcg acggcggcac gctgatcgtc gtggtcaagg
     5521 agcggccgtc gatcgagagc ttcacgatca ccggcaacaa ggacatcaag accgaggacc
     5581 tcgagaagtc gctgcgcaac gtcggcctcg ccaggggcaa ggtcttcaac cagtcgacgc
     5641 tcgacgaggt ggaccggttc ctgaccgacc agtactactc gcgcggcaag tacggcgtcg
     5701 tggtggacac gagggtcacc gacctgccga acaaccaggt gagcatcgcg atcgacgtca
     5761 aggagggcaa gcggtcgcgg atccggcaga tcaacatcgt gggcaacgag gcgttcagcg
     5821 aggaccagct gcgcgaagtc ttcaagctgc gtacgccgaa ctggctgtcc tggtaccgcg
     5881 acgacgacaa gtactcgcgc gaggagctct cgggcgacat cgagagcctg aagtcgttct
     5941 acctcgaccg cggctacgcc gacttcgacg tcgagacctc ccaggtggcg atcgccgcgg
     6001 agaaggacga catcttcatc acgctcaacg tgcacgaggg cgacgtctac cgggtgtcgg
     6061 acgtcaagat cgccggcgac ctggtggtgc ccgaggaaga cctgatgagc ctcgtccagg
     6121 tgcggcgggg cgacatctac tcgcgcaagt tcatcaccgc gaccacggag ctgatggcgc
     6181 tgcggctcgg gcaggacggc tacgcgttcg cgaagatcga cccggtgccg caggagaacc
     6241 cgaagaccaa ggaaatctcc atcacgttcc tggtgcagcc gggcagccgc gcctacgtca
     6301 ggcacatcaa cttcgaaggc gcgacgggca ccaacgacga cgtgttccgc cgcgaaatgc
     6361 ggcagatgga gggcggctac ctgtcgaacg cggccgtcga gcgctccaag cagcgcctgc
     6421 agcgcctgcc gttcatcgag aaggtggacg tcgagaccaa ccccgtgccg ggcacgcccg
     6481 acctggtcga cgtcgacttc aagatcaagg agggcatgcc ggggcagttc agcggcggca
     6541 tcggctactc gcagtcgcag tcgttcatgc tgaacgcgag cgtggtgcac agcaatttcc
     6601 tcggcacggg caaccgggtc gcagcggaag tgaacgccgg gcagtacagc caggtctaca
     6661 gcctctcgca caccgacccg tacgtcacgg tggacggcgt gtcgcgcacg ctgtcggcga
     6721 gctaccgcaa ctacaagcag tacaccagtt cgtcgtcgca gttcgacacg acgacgggcg
     6781 cgctcgccgt gaactacggc tggccgctgt cggaattcca gtcggtcgcg acgggcctgt
     6841 cggcccagca ctcggacctg ttcacggacc cgaaccagag cgcgcagcag tcgctgctct
     6901 gggtgcagca gaacggcaac ccgtacaccg agaagtcgac cgcgggcatg ccgccgacca
     6961 ccttcacgta ctacggcacg aaattcgaca cgttcgtgct gacggccggg tggagctacg
     7021 actcgcgcaa ccgctccatc ttcgcggatc gcggcgcccg ccacatcgtc aatctctcgt
     7081 acacggtccc gggcagcgac gtcgagttct actcgctcac ctacgactac ctgcagttcg
     7141 tcccgttgac gcgctggttc acgctgatga acaacttctc gacgggctac gcggccgcgc
     7201 tcggctcgac cacgtcgatt cccccgtacc ggaacttctt cgcgggcggc gcggaaagcg
     7261 tccgcgggta caaggccagc acgctgggcc cgaaggacac cttcggcaat ccgtacggcg
     7321 gcaacatcaa gctcaccaac cagttcgagc tgctgctgcc ggtgcccgag aaggtcaagg
     7381 gttcggctcg cgccagcctg ttcttcgacg cgggctacgt gttctcgagc gaggacgtga
     7441 agttctacgg cccgaacaac gtcacgcccg agaaatacga cttcgctttt gatatgcttc
     7501 gatattcgac cggcgtcgcc gtgcagtgga tggccccgat gggcatcttc cggttcagct
     7561 acgcgatccc gctcaatgcc cgcaagggca gcccgacggt ctacggcgac gagacggaaa
     7621 acttccagtt ctccattgga caggcttttt gaggaatacg gctccatgcg catcaagaca
     7681 gcgacccttt ccctgctggt cctcgccgca agcgccctgc ccgcgggcgg cgccctggcg
     7741 gacaccaagg tgggcgtcgt gaacgtgtcc cgcctgctgc aggagtcgcc gcaggcgcag
     7801 gccgcctcac aggcgctcga gagcgagttc gctgggcgtc gccgggagct cgaggcccag
     7861 cagaaggacc tgaaggccaa ggaagacaag ctgcagaagg atggcgcggt gatggcggcc
     7921 aacgagcgca ccaacgccga gaagacgctg cgcgacggcc agcgcgagct ggcgcgcaag
     7981 cagaacgagt tcatggagga cctgaacgtg cgccgcaacg aggcgctcgg gcagctgcag
     8041 cgcacggtgc tgcaggaagt ccaggcctat gcgaagacgg cggggctcga cgtggtggtc
     8101 gcggacgcgc tctatgcgag cccggcggtg gacatcacca accaggtgct cgccgcgttg
     8161 caggcgcgca agggcgccgc gccggccaag ccctgagccc cgggcggatc gcggggcgga
     8221 catgccagcg cggctcgccg aactggccgt ccgcttcggc tgcgaactgc gcggggaccc
     8281 cgacgccgtc gtcgagcgcg tcgcgtcgct gcaggacgcg gctcccggct cgctcacgtt
     8341 tctcgccaac ccccactatc gccgtcacct cgcgggcacg cgcgccacgg ccgtcgtgct
     8401 ggacgccgac tcggccgccg ggtgcccggt cgcggcactc gtgagccgta atccctacgc
     8461 atcgtacgcg cgcatggcgc aggtcctgca cccgccaccc gcggtcacgg gcggccgtca
     8521 cgcgtccgcc gtggtggacg agggcgcgga ggtcgatccg tccgcgtgga tcgggcccgg
     8581 cgcctacgtc gctcgcggcg cacgcatcgg cgctcgggcc agcatcgggc ccggctcgat
     8641 cgtgctcgac ggagcgcgcg tcggggacga cacgcggctc gtggcgcgcg tcaccctgtg
     8701 cgcaggggcg caggtcggca ggcgctgcct gctgcatccg gggtcggtga tcggcggcga
     8761 cggcttcggc cacgcgccgg acgccgacgg ctacgtgcgc gtgccgcagg tcggcagcgt
     8821 cgtcgtcggc gacgacgtgg acgtcggctg caattcgacg atcgatcgcg gcgcgatcgg
     8881 cgacacggtc atcgggaacg gcgtcaagat cgacaacctg gtgcaggtcg cccacaacgt
     8941 gcgcatcggg gagcacacgg tgcttgccgc gtacgtcggc atctcgggca gcgccacgat
     9001 cggccgccgc tgcatgctgg cggggatggt gggggtcgcg ggccacctcg agatctgcga
     9061 cgacgtggtg gtcaccggcc gcacgacggt cgcgggctcg atccgcaagg cgggcgtgta
     9121 ctccggcagc ctgacggcgg atgatgcgag gcgtttccgg cgcaacgccg cgcgcttcaa
     9181 ccagctcgac gacctggcgc gacgcctgcg caggctcgag ggagcggtgg agccggacga
     9241 cgggggaggg accgatgagt gaccagggtg gcttcggcat cgacgaggtg atgcggcgcc
     9301 tgccgcaccg ctatccgatg ctgctcgtcg accgcgtgct cgagtgcgtc ccgggcgagc
     9361 gcatcctcgc gctcaagaac gtcacgatga acgaggagtt cttctccggg catttccccg
     9421 ggcgtcccgt gatgcccggt gtgctgatcc tcgaggccat ggcccaggcc gcaggcatcc
     9481 tcacgttcgt gaccgccggc gtctatccgg acgactcggt ccgcttctat ttcgcaggca
     9541 tcgacaaggc gcgcttccgc cggccggtcg tgcccggcga ccaggtcatg ctctcggcga
     9601 cgctcgagcg gaggatccgc acgatctgga agtactcgac cctcgcgacg gtggacggcc
     9661 aggaggtcgc cagcgccgag atgatggtca tgccgggctg acggcccgcg atcgaaggcg
     9721 ggggcagcga atgtcaggtg cgatccatcc cacggccctg gtcgccgcgg acgcggaact
     9781 ggccgaggac gtgcgtgtcg ggccgttcgc ggtcatcggg gcgggcgtgc gcatcggacg
     9841 cggcacggtc gtcgggccgc acgccgtgat cgaaggcccg accgtcattg gcgagggcaa
     9901 ccggatcttc cagttcgcgt ccgtgggggc cgacccccag gacaagaaat accggggcga
     9961 ggtcacgcgc ctcgagatcg gcgaccgcaa cgtgatccgc gagtgcgcca cgatccaccg
    10021 cgggaccgtg caggaccagg gggtcacgcg gatcggcaac gacaacctgt tcatggccta
    10081 ttcgcacgtc gcgcacgact gcgtgatcgg cagccagtgc gtgctggcga accaggcgac
    10141 gctcgcgggg cacgtgcacc tcggcgactg ggtgatcatg ggcgggctgt ccgcgatcca
    10201 ccagttcggc aaggtcggag cccacgcctt catcgcgaac aacgccgcgg tgaccctcga
    10261 cgtgccgccg tacgtcatgg ccgtcgggca gcccgcgggc gcgcacagcg tcaacaccga
    10321 agggctcagg cgccggggat tctcgtcgga gcagatcggc aacatccgta acgcgttccg
    10381 gctgctgtac cggtcgggcc tgaagctcgc cgaggcgacg gaccgcctcg cggaactggc
    10441 cgcgggccag cccgagctgc ggccgctggt cgaattcctg ccgcaggcca cgcgcggcat
    10501 cgtccgctga gcggggcggc gcgtgatccc gagcgttgac gccgcgacgt tcggcctcgt
    10561 cgccggcgag gcttccggcg acaacctcgg cggcccgctg atccaggcgc tcgcggcgcg
    10621 tgcaaccgcg tcgcgattcg tcggcatcgc cggaccacgc atgctcgccg cgggctgcga
    10681 ggcctggcac gcgtcggacg agctcgccgt catggggctg gccgaggtgc tccggcacct
    10741 gccgcggctg gtgcgcctgc gccgtgacct gctggagcgt ttctcggcgg cgcggccgga
    10801 cgtattcgtc ggcatcgacg ctccggagtt caacttgcga gtcgccgcgc agctgaaggc
    10861 gcagggaatc gcgacggtgc agtacgtgag cccgcaggtc tgggcgtggc ggcaggggcg
    10921 cgtgcgcacg atcggcgagg ccgtcgacct cgtgctgtgc ctgctgccgt tcgaggtgga
    10981 tttctaccgc gcgcacggcg ttcgggccga gttcgtcggg catccgctgg ccgaccgggt
    11041 cccgctcgtg agcgatcgcg cggccgctcg ctcggcgctc ggcctgccgg ccgaaggatc
    11101 cgtcattgca gtactgcccg gcagtcgcca cggcgaagtg agccggctcg gtgcgccgtt
    11161 cgccgccacg atcgcgtggc tggccacgca gcgacccggg acccgattcg tcgcgccgat
    11221 ggccagtccc gcggcccgcg agtcgttcca gcattccctt gcgacccacg cgccgggcgt
    11281 cgaggtcgtg gtggtggacg gcgacgcgca gcgctgcatg gccgcgagcg acgtcgtgct
    11341 gctcgcgtcg ggcacggcga cgctcgaggc ggcgctcgtg aagcggccga tggtggtcgc
    11401 ctaccgcgtc gcgccgctca cgaactggct gctgcggggc ttgaggctgg tcaagaccga
    11461 atttttctcc cagccgaacc tgctcgcggg ccggcgcctg gtgcccgagc acttccagtc
    11521 ggaggtgcgg cccgacgtgc tggggccgtc cgtgctcgcg cagctcgagc gaccggacct
    11581 gcaggaactg acgggggcat tcacggacat gcaccgggcg ttgcggcgcg atgccagcgc
    11641 gcgcgcggcc gaagcgatcc tcgggctggc ggccgaacgg cgcggcgcgg catgagtgtg
    11701 cgccgcgacc cgcgccaggc gggcttcgac tggcgtcgcc ccgggctggt ggcgggcgtg
    11761 gacgaggcgg gtcgcgggcc gctggcgggg ccggtggtcg ccgccgccgt gatcctcgat
    11821 gcctcgaacc cggtcgacgg gctcgcggat tcgaaggtgc tcgcgccgtc gcgccgcgag
    11881 gaactcgacg cattgatccg cgagcgctgc gtggcctggg ggctggggtg ggccgacgcc
    11941 gccgagatcg actcgctcga catcctgcag gcgacgatgc tcgcgatgcg ccgtgcgctc
    12001 gcggcgctcg ccgtggcgcc tgcccacgtc atcgtggacg gcaatcgctg tccggacctg
    12061 cggggcatcg atctcgagtg cacgatcgag gcggtcgtca agggcgacgc gacggtcgcg
    12121 tgcgtcagtg ccgcttcggt gatcgcgaag gtcgcgcgcg acgcgcacat gcgcgagctg
    12181 gacggcctct acccgggata cggcttcgcg gagcacaagg gatacccgac ggcggcgcac
    12241 gtcgcggcgc tgcgccggct cggcccgtcc gccgtgcacc ggcgcagctt cgccccggta
    12301 caatcgctgg acacacgctg atacccatcc gcatggctcg cttcgtccac ctgcgcgtgc
    12361 acaccgagta ttcgctcgtc gacggcgtcg tgcgcgtcga gagcgagcac aaggacggca
    12421 aggtcgtgcg cgaggggctg gtggacgcct gcgcgcggct cggcatgccc gcggtggcgg
    12481 tcaccgacca gggcaatctc ttcgcgctcg tgaagttcta cactgccgcg caggcgcggg
    12541 gcgtgaagcc gctggtgggc gtggacgcgc tgctcagcga ggagggtgag cgcgccgagc
    12601 ccagccggct ggtgctgctg tgccaggacg agcgcggcta ccgcaacctc acgcggctgg
    12661 tgacgcggtc ctacctcgag ggacagggac gacacgggcc gaccctgcgg cgttcgtggc
    12721 tggacccggc atcgaccgcg ggcctgatcg cgctgtccgg cggtcgcgaa ggcgacgtcg
    12781 gccgcgcatt gctcgccggt cgcgaagagg acgcccgctc gacggtcgca tcctggctcg
    12841 aactgttcgg tgaccgttac tacctcgaac tgcagcgcac gggccggccg ggcgaggaag
    12901 aatgcatcgc cggttcgctg gcgctcgcga cggcgctcgg cgtgccggtg gtcgcgacca
    12961 acgacgtccg cttcatccgc cgcgacgact tcgagtcgca cgaggcgcgc gtctgcatcc
    13021 acgacggcac gctgctcgcc gacccgtccc ggccgcggcg ctacagcgag gagcagtacc
    13081 tgcgctcgcc ggaggagatg gcgaagctct tcgccgacgt gcccgaggcg ctggagaact
    13141 ccgtcgagat cgccaggcgg ctcaacctgg aactgcggct cggcaaggcg tcgctgcccg
    13201 cgtacccggt gccggccggg cagaccaccg aggaatacct ccgcgccgaa gcgcgcagcg
    13261 gcctcgaatc gcgcctgtcc gccgcagctg cggcacccgg gctggctccg gcaatcgagc
    13321 gcgcgccata cgaggcgcgc ctcgcgaccg agctcgacgt gatctgccag atggggttcg
    13381 cgggttactt cctgatcgtc gcggacttca tccgctgggc gcgcgacaac ggcgttcccg
    13441 taggaccagg cagaggttcc ggggctggct cgctggtcgc ctgggtgctc ggcatcaccg
    13501 acctcgaccc gatccgccac gacctgctgt tcgagcgctt cctgaatccc gagcgcgtgt
    13561 cgatgccgga cttcgacgtg gacttctgca tggaaggccg cgatcgcgtg atcgagtacg
    13621 tggcggagcg ttacgggcgc gagcgcgtct cgcagatcat tacctacggc acgatggccg
    13681 cgaaggccgt cgtgcgcgac gtcgcgcgcg tgctcgcgct gtcgtacggg ttcgcggacg
    13741 gcatcgcgaa gctcatcccg ttcgagctcg gcatcacgct cgacgacgcg ctcgagaagg
    13801 aggaggagct gcgcaggcgc tacaagtccg aggacgaggt gcgcgaggtg atcgacctcg
    13861 cccggtcgct
//
LOCUS       DAOMFD010000042        13705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1064_length_13705_cov_30.779634, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000042 DAOMFD010000000
VERSION     DAOMFD010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1064_length_13705_cov_30.779634"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..185
                     /locus_tag="PL152_04085"
     CDS             <1..185
                     /locus_tag="PL152_04085"
                     /inference="COORDINATES: protein motif:HMM:NF012546.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQR48489.1"
                     /translation="KMAARLQAASASGRPILLRVDFDAGHGMGSTREQQDAEAADTYA
                     FILWQTRRNPAGAPAR"
     gene            complement(215..1387)
                     /locus_tag="PL152_04090"
     CDS             complement(215..1387)
                     /locus_tag="PL152_04090"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR48490.1"
                     /translation="MDKTRPTGNAYTLWGGALSLYTGKVRSYLIKTGIPYREYYTCHP
                     EYRERVFRTVQLGVAPILESPDGDTVQDTTDIIEYLERKHPATSMIPRTPVQRAVAWL
                     ICAFGSDGLLPAAMHYRWSYKDEQRLWLLDEFGRFSSSGTRAERHEAAGKFMARMDAM
                     LPALGVTRESAPAIEASHRAMLAILDEHFLAHPYLLGGRPSIADFGMMAAMFAHLGRD
                     PVPAHLMKLTAPNVYRWTERMNLAGIFDPEFPDTPDAWLADDAIPKTLLPLLAHIFGD
                     WGPELLASAAHYNEWVRVHPDLPAGTPPSLVTGRRVVHPMLGKVSYPYRGITMERHCA
                     ANSLWHFEKSAAIARTFEGNVRACWQALLAETGGAEMMAIRLARPMKRENYLLVLA"
     gene            1523..2314
                     /locus_tag="PL152_04095"
     CDS             1523..2314
                     /locus_tag="PL152_04095"
                     /inference="COORDINATES: protein motif:HMM:NF012596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HQR48491.1"
                     /translation="MIDSDQLLVERDGGVLILTINTPPMNRMTFEFMDEFERCVTAAA
                     ADDSVRCLLITACGEENFSVGMDLKQLASVAADLSRVAAVLDQRLRVLALIENMAKPS
                     VVTMYGHCLGGGLELPLACHFRLAAAEGARIGLPELDLGTVPAWGGTARLTRRVGRDR
                     ALDMILRARKIDGPEALRIGLVHEVHPLVRLKPAARELAQQLASMPPIAVAGVLACVV
                     GAGESSLDAALVAERRAFLRCAGSEDQAEGLRAFIEKRKPVFRGR"
     gene            2485..2637
                     /locus_tag="PL152_04100"
     CDS             2485..2637
                     /locus_tag="PL152_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48492.1"
                     /translation="MKSHPILASIVAGIALASGAHAQQQQQAVQQHQAAQQQQMDLFR
                     KGYTVK"
     gene            2669..3466
                     /gene="ygiD"
                     /locus_tag="PL152_04105"
     CDS             2669..3466
                     /gene="ygiD"
                     /locus_tag="PL152_04105"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF007914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HQR48493.1"
                     /translation="MTPAGTAMPAAFLGHGSPMNALERNRYTEAWRAFGASVPRPRAI
                     LMVSAHWYVNTTAVTTMARPKTIHDFYGFPRELFAIQYPAPGLPELFGEITEVVKPGG
                     VAADPDSWGLDHGAWSVLLHAFPAADIPVVQLSIDTRQRPEYHLELGAKLAGLRDRGV
                     LLMGSGNIVHNLRAIDWNRPDGAYDWALRFNEQACEVLAERPADVVGLMEHPDFAAAS
                     PTPDHFLPLLYIAGFAAASGARMDLQLRDAYAYGSLSMTCCVAQAAI"
     gene            3680..3772
                     /gene="kdpF"
                     /locus_tag="PL152_04110"
     CDS             3680..3772
                     /gene="kdpF"
                     /locus_tag="PL152_04110"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HQR48494.1"
                     /translation="MNASDVVSLVLVIALGVYLVIALLNPEKFQ"
     gene            3778..5574
                     /gene="kdpA"
                     /locus_tag="PL152_04115"
     CDS             3778..5574
                     /gene="kdpA"
                     /locus_tag="PL152_04115"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HQR48495.1"
                     /translation="MGLLQIVLYLVVLVALARPLGAYVARIYAGERTWLTPLIAPLER
                     LTYRIAGVDPAREQDWKQYTLAVLAFNFAGLVVVYLLQRLQGVLPLNPQAFGAVSADS
                     AFNTAVSFATNTNWQGYGGETTLSNLTQMLGLGVQNFVSAASGMAVLVALIRGFVRRE
                     SGVIGNFWVDLTRSTLYVLLPLSTLLAIVLVSQGVVQSFAPYREVSLVEPVTVQAPAT
                     NDAGQPIADADGRPVVQPQRITTQTIALGPAASQVAIKQLGTNGGGFFNVNSAHPFEN
                     PTPLANFLEMLAILLIPAALCYTFGRMVGDTRQGWAVLAAMLVIFVPLALAGYAAEQA
                     GNPRLDPTAVDQQASANHAGGNMEGKEVRSGIANSALWAAATTAASNGSVNSMHDSYT
                     PLGGLVPMWLMQLGEVVFGGVGSGLYGMLMFAIIAVFIAGLMVGRTPEYLAKKIEAYE
                     VKMASLVLLIPCFIVLAGTAIACVTPHAVSKIANPGAHGFSEILYAFSSAGNNNGSAF
                     AGLSANSPFYNVALGIAMWLSRYWLMIPVLAIAGSLAAKRAVAVTSGTLPTHTPLFVT
                     LLVGVVLLVGALTFVPALALGPIVEQLQMIHP"
     gene            5586..7670
                     /gene="kdpB"
                     /locus_tag="PL152_04120"
     CDS             5586..7670
                     /gene="kdpB"
                     /locus_tag="PL152_04120"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HQR48496.1"
                     /translation="MTHTANDRRTFLSGSIVTAGLLEALRKLDPRVQVRNPVMFVVYV
                     GSILTTAIGFLAAVSTDAAEFGRAGFVLAIAGWLWLTVLFANFAEAVAEGRGKAQAAA
                     LRSTRRDVQAKRLDARTRNGSHQIVSANALRKGDYFLVEAKETIPADGEVVEGVASVD
                     ESAITGESAPVLREAGSDFSSVTGGTRVLSDWLVVRVTSDPGESFLDRMISMVEGARR
                     GKTPNEIALSILLAMLTLVFLLATATLSPFSAYAVAAAGTGQVVSVVVLVALLVCLIP
                     TTIGGLLSAIGIAGMDRMVRANVIATSGRAIEAAGDVDVLLLDKTGTITLGDRQAAAF
                     HPTPGVTEQELADVAQLASLSDETPEGRSIVVLAKQRFQLRGRDLSTQAHVFYKFTAQ
                     TRTSGVDLEGRKLRKGAADAIRRFVEGEHGVWPAAVTRSVDDVARHGATPLVVADGAR
                     VLGVVELRDVVKGGIRERFSELRQMGIATVMVTGDNRLTAAAIAAEAGVDDFLAEATP
                     ETKLALIRKYQSEGHLVAMCGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSN
                     PTKLIEVVDVGKQMLVTRGALTTFSIANDVAKYFAIIPAAFAATYPSLDALNVMGLAS
                     PQSAILSAVIFNALIIVVLIPLALRGVKTRAASAAQLLRRNLLVYGLGGIVVPFVGIK
                     LIDVLLATMNVV"
     gene            7680..8252
                     /gene="kdpC"
                     /locus_tag="PL152_04125"
     CDS             7680..8252
                     /gene="kdpC"
                     /locus_tag="PL152_04125"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HQR48497.1"
                     /translation="MNGVFRQCLGVFVMLTLVTGVAYPLFITGVAKLAFPGEASGSII
                     VRDGRAVGSELLGQSFSGRGYFWGRPSATTPQPYNAGASAGSNAGPTNPALSDAVAAR
                     VAALRAADPGNDAPVPVDLVTASGSGLDPHISPAAAEYQVARVARARGWTPDRVRELV
                     VAHTEDRTFGVLGEPRVNVLRLNLALDSAR"
     gene            8254..10947
                     /locus_tag="PL152_04130"
     CDS             8254..10947
                     /locus_tag="PL152_04130"
                     /inference="COORDINATES: protein motif:HMM:NF014728.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HQR48498.1"
                     /translation="MADATRPDPDRLLADVQRDEARAKRGRLRIFFGASAGVGKTYSM
                     LEAARAARNTGADVVVGYVEPHGRVETERLLEGLEQIPMLPVRYRGVTRQEFDLDAAM
                     RRRASITLVDELAHSNLVEGDPAPRHPKRWQDVEEMLEAGLDVWTTLNVQHIESLNDV
                     IAGITGVRQQETVPDHVLEDAAEIELIDLPPEDLLTRLREGKVYVPEQVGAALERFFR
                     KPNLLALRELALRQTADRVDAAARQYTTREPVSRPWLARERFIVGVGPDDQGEELVRF
                     GKRFADALDAEWIVVSVETPPLMRLGEAARDRRIAVLRLAESLGAETVTLDGPTAASA
                     LLQYARTRNVTRIVVGEPKRRGLRTLWRRSTANELVRRAAGIDVSVIARRTVAARPDV
                     PPVARLEPPTVHWERYAWAAGIVVLCTLVVWAAQDFLALTNLVMVYLVGTTVAGLRLG
                     RGPSILASVLGVAAFDFFFVPPPYTFAVSDVEYLVTFLVMLIVGLTIATLMANVRQQT
                     RIAGARERRTALLYAMSRELAGTRGPRKMAQAAVRHVSETFSSRAAILLPDVDGGLEH
                     VDAGDEDSRFAYPDLSVAQWVFDHGRAAGLGTDALPGAPAIYLPLAGTDRTLGVLAVL
                     PANNRRVLLPEQRHLLETFASQIALAIERAVLGEAAQSAKIAAETEALRNTLLASISH
                     DLRTPLAVIAGAGSALADPKLALDPDARTALARTIATKSQEISDLVSNVLDLMRFESG
                     HVPLRLQWETLDDLVGLALGKVESRLGRRPVHVMLPPELPGVNVDAVLITQVLVNLLE
                     NAAKHTPEGTHVWISGHADGAGVQVVVEDDGPGLPPGDPDLLFAKFQRGRTESEVPGA
                     GLGLAICRAIVEAHGGHVTASNRDGGGARFVLTLPVEATRA"
     gene            10944..11657
                     /locus_tag="PL152_04135"
     CDS             10944..11657
                     /locus_tag="PL152_04135"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR48499.1"
                     /translation="MTDAMFRLLVVEDDRDIRELLRTMLEAQGYRVVLAESAARALVE
                     ARSNKPDALLVDLGLPDRDGQDLIRDVRAFSTVPIIVLSARAMESEKVLALDGGADDY
                     VTKPFQSNELLARIRAALRRSVRSADQSQVLEIGDVVINLETREAQSPRGAVHLTPVE
                     FRLLACLARNLGLVVTQEQVIRDVWGPDRVTDTRGLRSYVKTLRQKLEPDPSRPRFLL
                     TEPGLGYRMVADSPVAPRT"
     gene            complement(11615..>13705)
                     /locus_tag="PL152_04140"
     CDS             complement(11615..>13705)
                     /locus_tag="PL152_04140"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR48500.1"
                     /translation="FERVGWDEALDDIARRLVDIRDRQGGKAFAFVGGAGQGNHLGGA
                     YSRQLLPAMRSKFAYNSLGQEKTGDFWVNGRLFGHQTCHTTEDVEHADYVLFIGCNPF
                     QSHGIPSARDTLRALRKDTQRTMVVVDPRRTETAEQADIHLQLRPGTDAHLLAAMLAI
                     MVRDGLIDRGFLAQHCSGFDGVERHLREVPIEEYVRRADVPLADVERVARGFATARKA
                     CVRVDLGTQHTLHTTLNAYLEKLLWLVTGQFGRAGTNNLHTHLLPILGHTDERVRIEG
                     KPLPRTAHHRMHPIAGVFPPNILPDEILLAGEDRVRAVFVDSCNPLMTWADTAAYEKA
                     FGSLELLVVVDVAMTETAKLAHYVLPAASQFEKWEATGFNLDFPENYFHLRHPLFEPL
                     GETLPEPEIYTRLLEKMGAIPRRFPVLSSVARLEPDAAQYLPYMVLLAATLATHKQWV
                     PYAASIAYRTLGSALPNGAAAAAFILPLAMQYASRHEVAVRRAGHRGGRLTLGSRLFQ
                     AILEQRSGVVLSRHDYEEVWSLVRTPDKRIRLAIPEMLAELDGLSSESLAVESFPFIL
                     MAGERRSYNANQIFRDPAWRKVDKDGAMRVHPADAANIGVVNGDRAVVQSASGEIDVS
                     IEIDDSVRPGVVTLPHGYGGQFDGRGPIGPALNRLTSSSHCDPFTRTPYHKYVPVRIR
                     RQVRGATGESATIR"
BASE COUNT         2098 a         4756 c         4599 g         2252 t
ORIGIN      
        1 ccaagatggc ggcgcgcctg caggccgcga gcgccagcgg caggccgatc ctgctgcgag
       61 tcgacttcga cgccggccac ggcatgggct cgacgcgcga acagcaggac gccgaggccg
      121 ccgacactta cgcgttcatc ctctggcaga cacgcaggaa ccccgccggc gcaccggccc
      181 gctgactgtc accacccgtg gctgcgtaca cgattcacgc gagcacgagc aggtagttct
      241 cgcgcttcat cgggcgtgcc aggcggatcg ccatcatctc ggccccgccg gtctccgcaa
      301 gcagtgcctg ccagcaggcg cgcacgtttc cctcgaacgt gcgtgcgatg gccgcggact
      361 tttcgaagtg ccacagcgag ttcgccgcgc agtgccgctc catggtgatc ccgcggtacg
      421 ggtagctcac cttgcccagc atcggatgca ccacgcggcg tcccgtcacg agcgaaggcg
      481 gcgtgcccgc gggcaggtcg ggatgcaccc tcacccactc gttgtaatgc gccgccgacg
      541 cgagcagttc gggaccccag tcaccgaaga tgtgcgcgag aagcggcagc agcgttttcg
      601 ggatcgcgtc gtctgcgagc caagcgtcgg gcgtgtccgg gaactccgga tcgaagattc
      661 cggcgaggtt catgcgctcg gtccagcggt agacgttcgg cgccgtgagc ttcatgaggt
      721 gcgccgggac gggatcgcgc cccaggtgcg cgaacatcgc cgccatcatg ccgaagtcgg
      781 cgatgctggg gcggccgccg agcaggtacg ggtgcgcgag gaaatgctcg tcaaggatcg
      841 cgagcatcgc gcggtgcgac gcctcgatcg ccggcgcaga ctcccgcgtg acgccgagcg
      901 ccggcagcat cgcgtccatc cgtgccatga acttgccggc cgcctcatgc cgttccgcgc
      961 gagtgcccga gctgctgaac cggccgaatt cgtccagcag ccacagccgc tgctcgtcct
     1021 tgtacgacca gcggtaatgc atggccgccg gcaggagtcc gtcggacccg aatgcgcaga
     1081 tgagccaggc gacggcccgc tgcacgggcg tccgcggaat catcgaagtc gccgggtgct
     1141 ttctctcgag gtactcgatg atgtcggtcg tgtcctgcac cgtgtcgccg tcgggcgact
     1201 cgaggatcgg cgccacgccc agctgcacgg tcctgaagac gcgctcccgg tattccgggt
     1261 ggcaggtgta gtactcgcga tacggaatcc cggtcttgat caggtaagaa cgtactttgc
     1321 cggtgtagag ggacagtgcg ccgccccaca acgtgtacgc gttacccgtg ggcctggtct
     1381 tgtccactca actctccggt gcaacgtcgg cgggatcgtc gcatggtata cgacgtgttt
     1441 cgccggtgcc ctgcgtacgc atactgacat cgcgaactgc gtcggtccgc taccatggcc
     1501 gccccccaac gaggaggcgc ccgtgatcga ttccgaccag ctgctcgtcg aacgcgacgg
     1561 tggcgtgctg attctcacga tcaacacccc gccgatgaac cgcatgacct tcgagttcat
     1621 ggacgaattc gagcgctgtg tcacggccgc cgccgcagac gattccgtcc gctgcctgct
     1681 gatcacggcg tgcggcgagg agaacttttc ggtcggcatg gacctcaagc agctcgcatc
     1741 ggtcgcggct gatctctcgc gggtggcggc cgtcctcgac cagcggctgc gcgtgctcgc
     1801 cctgatcgag aacatggcga aaccctccgt cgtcacgatg tacggccact gcctgggggg
     1861 cggcctcgaa ctgccactcg cctgccactt ccggctcgcg gcggccgaag gcgcgcggat
     1921 cgggctgccc gagctcgacc tcggcacggt tcccgcctgg ggcggtacgg cccggctcac
     1981 gcgacgcgtc ggccgcgacc gcgcgctcga catgatcctg cgtgcgcgca agatcgacgg
     2041 accggaggca ttgcgcattg gactggtaca cgaggtgcat ccgctcgttc gcctgaagcc
     2101 cgctgcccgc gagctggcgc agcagctggc cagcatgccg cccatcgccg tcgccggcgt
     2161 gcttgcctgc gtggtcgggg ccggcgagtc gtcgctcgac gcagcgctcg tggcggaacg
     2221 ccgcgcattc ctgcgctgcg cgggcagcga ggaccaggcc gaaggcctgc gcgccttcat
     2281 cgagaagcgc aagccggtct tccgcgggcg ttgactcccg gcgcaggaac gtgccgcgcc
     2341 ctgccggcgg tacggtcatc gactgccact tggaagtccg gtcttcctgt aacatcaggc
     2401 cgctcgagcc gccctgtcgc gacgatcgca cgtggggtcg gtcgcaaggc ctcccggcat
     2461 caaccacaca cccaggttac attcatgaag tcgcatccga ttctggccag catcgttgcc
     2521 ggcattgcat tggcgtcggg cgctcatgcc cagcagcaac agcaggcggt ccagcagcac
     2581 caggcggcgc aacagcagca gatggacctg ttccgcaagg gctacaccgt caagtgagga
     2641 cgcgcttcgg tcgcgcacgg agcccgcggt gaccccggca ggaaccgcga tgccggcggc
     2701 gttcctggga cacggcagtc ccatgaacgc gctcgagcgc aatcgctaca cggaggcgtg
     2761 gcgtgccttc ggcgcgtcgg tgccgcgccc gcgcgcgatc ctgatggtct ccgcgcactg
     2821 gtacgtgaac acgaccgccg tcaccacgat ggcacgcccg aagacgatcc acgatttcta
     2881 cggctttccc cgggaactct tcgcgatcca gtacccggcg ccgggactgc ccgagctctt
     2941 cggtgagatc accgaggtcg tgaagccggg aggcgtcgcc gccgacccgg acagctgggg
     3001 cctcgaccac ggggcgtggt ccgtcctgct gcatgcattt ccggcggccg acattcccgt
     3061 cgtccagctc tccatcgata cgcgccagcg gccggagtat cacctcgagc tcggcgcgaa
     3121 actcgccggg ctccgtgacc gaggcgtgct gctcatgggc agcggcaaca tcgtgcacaa
     3181 ccttcgcgcc atcgactgga atcgccccga cggggcctac gactgggcct tgagattcaa
     3241 cgaacaggcc tgcgaggtcc tggccgaacg tccggccgat gtggtcgggt tgatggaaca
     3301 tccggacttt gccgcggcct cgccgacacc cgaccacttc ctgccgctgc tttacatcgc
     3361 aggattcgcg gccgcgtcgg gcgcgagaat ggacctgcag ctgcgcgacg cctacgccta
     3421 cggctcgctg tcgatgacct gctgcgtggc gcaggccgcc atttaggcgc acgccgggcc
     3481 gcctcggccg gccacccatc gctcgatttt tcacacgatt ccgcctccac gcggtctcgg
     3541 cacgggcaca ccatgccgaa caggtgatcc gcgttcgccg gaggattgcg atggaaatct
     3601 ggatgatcct ggtcacggcc gtcctggtcg tgacgaccgt cggcctgtat cggctcgtcg
     3661 accggctgcg agcaaagcca tgaacgccag cgacgtcgtg agcctggtgc tcgtcatcgc
     3721 gcttggcgtc tacctcgtga tcgccttgct caatccggag aaattccagt gaccgggatg
     3781 ggcctgctgc agatcgtgct gtacctcgtc gtgctggtcg cgctggccag gccgctcggc
     3841 gcctacgtgg cgaggatcta tgccggggag cgtacctggc tcacaccctt gatcgctccg
     3901 ctcgagcggc tcacctatcg catcgcgggc gtcgatccag cccgcgagca ggactggaag
     3961 cagtacacgc tcgcggtcct ggcgttcaac ttcgcgggcc tcgtcgtcgt gtacctgctg
     4021 caacgactgc agggcgtcct gccgttgaat ccgcaggcct tcggcgcggt gtcggcggac
     4081 tcggcgttca acaccgcggt cagtttcgcg accaatacca actggcaggg ttacggcggc
     4141 gagacgacgc tgagcaacct cacccagatg ctgggtctcg gcgtccagaa tttcgtgtcc
     4201 gccgcctcgg gcatggcggt gctcgtcgct ctcatccgcg gcttcgtccg gcgtgaatcc
     4261 ggcgtcatcg gcaacttctg ggtggacctg acacgcagca ccctctacgt gctgctgccg
     4321 ctgtccacgc tgctggcgat cgtgctcgtg agccagggcg tcgtgcagag cttcgcgccg
     4381 tatcgcgagg tgtcgctcgt cgagcccgtg accgtccagg cgccggcgac caatgatgcc
     4441 ggacagccca tcgccgacgc ggacggccga cccgtcgtgc agccgcagcg gatcaccacc
     4501 cagaccattg ctttgggtcc ggctgcaagt caggtcgcga tcaagcagct cggcacgaac
     4561 ggcggcggct tcttcaacgt caactccgcg cacccgttcg agaacccgac gccgctggcc
     4621 aacttcctcg agatgctggc gatcctgctg atcccggctg cgctctgcta cacgttcggc
     4681 cgcatggtcg gcgacacgcg ccagggctgg gcggtgctcg ccgcgatgct cgtgatcttc
     4741 gtgccactgg cgctcgccgg atacgcggcc gagcaggccg gaaatccgcg cctcgatccg
     4801 accgccgtgg accagcaggc gtcggcgaac catgcgggcg gcaacatgga gggcaaggaa
     4861 gtgcgctccg gcatcgcgaa ctccgcgctg tgggccgcgg ccacgaccgc ggcctccaac
     4921 ggctccgtga actcgatgca cgactcctac acgccgctcg gtggcctcgt gccgatgtgg
     4981 ctgatgcagc tcggcgaggt cgtgttcggc ggcgtcggca gcggcctcta cggcatgctc
     5041 atgttcgcga tcatcgccgt gttcatcgcc ggactcatgg tcgggcgcac gcccgagtac
     5101 ctcgccaaga agatcgaggc gtacgaggtc aagatggcct cgctggtgtt gctgatccca
     5161 tgcttcatcg tgctcgccgg cacggcgatc gcctgcgtga ccccgcatgc cgtctcgaag
     5221 atcgccaatc ccggcgccca cggcttctcc gagatcctct atgcgttcag ctccgcaggc
     5281 aacaacaacg gcagcgcctt cgccggcctg tccgcgaaca gccccttcta taacgtcgcg
     5341 ctgggcatcg cgatgtggtt gtcgcgctac tggctcatga tcccggtgct ggccatcgcg
     5401 ggcagcctgg ctgccaagcg agccgtcgcg gtgacatcgg gaaccttgcc gacgcacacg
     5461 cccctgttcg tcacgctgct cgtcggcgtg gtgctgctcg tcggcgcgct caccttcgtg
     5521 ccggcgctcg cgctcgggcc gatcgtggaa caactgcaga tgatccaccc gtgagaccgc
     5581 cggccatgac ccacacagcc aacgatcgcc gcaccttcct gagcgggtcc atcgtcaccg
     5641 cggggctcct cgaggcactc cgcaagctgg acccgagggt ccaggtccgg aacccggtga
     5701 tgtttgtcgt gtacgtcggc agcatcctca cgaccgcgat cggcttcctt gcggcggtct
     5761 cgacggatgc cgctgaattc gggcgcgccg gcttcgtgct cgcgatcgcc ggctggctgt
     5821 ggctcacggt cctgttcgcc aatttcgccg aggccgtcgc cgaggggcgc ggcaaggcgc
     5881 aggccgcggc gctgcggtcc acgcgtcgcg acgtgcaggc gaagcgcctc gacgcccgaa
     5941 cgcgcaatgg aagccaccag atcgtctccg cgaatgcgct gcgcaagggc gattacttcc
     6001 tggtcgaggc aaaggagacc atcccagccg acggcgaggt cgtcgagggc gtcgcgtcgg
     6061 tcgacgagag cgccatcacc ggcgagtccg cccccgtgct gcgcgaagcc ggcagcgatt
     6121 tttcctcggt caccggtggc acgcgcgtgt tgtctgactg gctcgtggtg cgcgtgacga
     6181 gcgacccggg cgagtccttc ctcgaccgga tgatctctat ggtcgagggc gcacggcgcg
     6241 gcaagacgcc gaacgagatc gcgctgtcga tcctgctcgc gatgctgacc ctggtgttcc
     6301 tgctcgccac ggcgaccctg tcgccattct ccgcgtacgc ggtcgcggcc gccggcacgg
     6361 gacaggtcgt gtctgtcgtc gtgctggtcg ccctgctcgt atgcctgatt ccgaccacta
     6421 tcggcggcct gctgtccgca atcggcatcg ccggcatgga ccggatggtg cgtgccaacg
     6481 tcatcgccac gtccggccgc gccatcgagg ccgcgggcga cgtggacgtc ctcctgctcg
     6541 acaagaccgg cacgatcacc ctcggcgacc ggcaggccgc ggctttccat cccaccccgg
     6601 gtgtcaccga gcaggagctc gccgacgtgg cgcagcttgc gtcgctgtcg gacgagacgc
     6661 ccgagggccg gtccatcgtg gtgctcgcga agcagaggtt ccagctgcgc ggccgcgacc
     6721 tctcgacaca ggcgcacgtc ttctacaagt tcaccgcaca gacccgcacc agtggcgtgg
     6781 acctcgaggg ccgcaagctg cgcaagggcg cggccgacgc gatccgccgc ttcgtggaag
     6841 gcgagcacgg cgtgtggcca gccgcggtga ctcgctcggt cgacgacgtt gcccggcacg
     6901 gcgcgactcc tctcgtggtc gccgacggag ccagggtgct cggcgtcgtc gagttgcgcg
     6961 acgtcgtcaa gggtggcatc cgggagcggt tctcggagct gcgtcagatg ggcattgcga
     7021 cggtgatggt gaccggcgac aaccgcctga ccgcggcggc catcgctgcc gaggccggcg
     7081 tggacgactt cctggccgag gcgacgcccg agacgaagct cgcgctgatc cgcaagtacc
     7141 agtcggaggg ccacctggtc gcgatgtgcg gcgacggcac gaacgatgcg cccgccctcg
     7201 cccaggccga cgtcgccgtg gcgatgaact ccggcaccca ggcggcgaag gaagccggca
     7261 acatggtcga cctcgattcg aacccgacca agctcatcga ggtcgtggac gtcggcaagc
     7321 agatgctcgt gacgcgcggc gcgctcacga ccttctcgat cgcgaacgac gtcgccaagt
     7381 acttcgcgat catcccggcc gccttcgcgg cgacctatcc gtcgctcgac gccctcaacg
     7441 tcatgggact cgcgagcccg cagagcgcga tcctgtcggc cgtgatcttc aacgcgctga
     7501 tcatcgtcgt gctgatcccg ctggccctgc gcggcgtcaa gacccgcgcg gcctccgccg
     7561 cgcagctcct gcgtcggaat ctcctcgtct acggtctcgg cggcatcgtc gtgccgttcg
     7621 tcggcatcaa gctgatcgac gtgctgcttg cgaccatgaa tgtcgtttga ggtaaggtaa
     7681 tgaacggcgt ctttcggcag tgcctcgggg tgttcgtcat gttgacgctg gtcactgggg
     7741 tggcctatcc gctgttcatc accggggtcg cgaagctggc gttccccggg gaggccagcg
     7801 gcagcatcat cgttcgcgac ggccgcgcgg tgggttccga attgctcggg caatcgttct
     7861 caggtcgggg ttatttctgg gggcgtccgt cggccaccac cccccaaccg tacaatgccg
     7921 gcgcctccgc tggctccaat gccggaccga ccaacccggc actgtcggac gccgttgcgg
     7981 cccgtgtcgc ggcattgcgc gcggccgacc ctggcaacga tgctcccgta cccgtggacc
     8041 tcgtcaccgc gtctggcagc ggcctcgacc cgcacatttc gccggcggcc gcggagtacc
     8101 aggtcgcccg ggtcgcgcgc gcccgtggct ggacgccgga cagggtgcgc gaactcgtgg
     8161 tggcacacac ggaagaccgc acgttcggcg tcctcggcga accccgggtg aacgtgctgc
     8221 gactcaacct ggcgctcgat tccgcgaggt aaggtggccg acgctacgcg acccgacccc
     8281 gaccggctgc tcgccgacgt ccagcgtgac gaggcgcgcg cgaagcgcgg gcgcctgcgc
     8341 atcttcttcg gcgcatcggc cggagtcggc aagacctact cgatgctcga ggccgcgcgt
     8401 gcggcgcgca acaccggcgc cgacgtcgtc gtaggctacg tcgaacccca tgggcgcgtg
     8461 gagacggaga gactgctcga gggtctcgag cagatcccaa tgttgccggt ccgctaccgc
     8521 ggggtcacgc ggcaggaatt cgacctggat gcagcgatgc gccggcgagc ttcgatcacg
     8581 ctggtcgacg aactcgcgca ttcgaacctg gtcgagggcg acccggcacc gcgacacccg
     8641 aagcgctggc aggacgtcga ggagatgctc gaggccggcc tcgacgtgtg gaccacgctc
     8701 aacgtccagc acatcgagag cctgaacgac gtgatcgccg ggatcacggg cgtccggcaa
     8761 caggaaacgg tgcccgacca tgtgctggag gatgccgccg aaatcgagct gatcgacctc
     8821 ccgccggaag acctgctcac gcgcctcagg gaaggcaagg tgtacgtgcc ggaacaggtc
     8881 ggcgcggccc tcgagcgatt cttccgcaag ccgaacctgc tcgcgttgcg cgaactcgct
     8941 ctgcggcaga ccgcggaccg cgtcgacgcg gctgcacgcc agtacacgac tcgcgaaccg
     9001 gtgtcgcggc cgtggctcgc tcgcgaacgg ttcatcgtcg gcgtggggcc cgacgaccag
     9061 ggcgaggagc tggtgcgctt cggcaagcgc tttgccgacg cgctcgatgc cgagtggatc
     9121 gtcgtgtccg tcgagacccc gccgctcatg cggctcggcg aggcggctcg cgaccgccgc
     9181 atcgcggtct tgcgcctggc cgagtcgctc ggcgccgaga cagtcacact ggacggcccg
     9241 acggcggctt ccgccctgct gcagtacgcc cgcacgcgca acgtgacgcg catcgtcgtg
     9301 ggcgagccga aacgacgtgg gttgcgcaca ctgtggcgac gatcgacggc gaacgagctg
     9361 gtgcggcgag ccgccggcat cgacgtgtcc gtcatcgcgc gacgcacggt cgcggcgcgc
     9421 ccggatgttc ccccggtcgc gcgactcgag ccaccgacgg tgcactggga gcgctatgcc
     9481 tgggcggccg gcatcgtcgt gctgtgcacg ctcgtggtct gggcggcgca ggatttcctg
     9541 gccctgacca acctggtcat ggtgtacctg gtcggcacca ccgtcgcggg actgcgcctg
     9601 ggccgaggac cgtcgatcct cgcatccgtg ctcggcgttg ccgcattcga cttcttcttc
     9661 gtgccgccgc cctatacgtt cgcggtttcc gacgtcgagt acctcgtgac gtttctcgtc
     9721 atgctgatcg tcgggctcac gatcgcgacg ctcatggcga acgtgcggca gcagacgcga
     9781 atcgccgggg cgcgggagcg gcggacggca ttgctctacg ccatgagtcg cgaactcgcc
     9841 ggcacgcgtg ggcccaggaa aatggcccag gccgcggtca ggcacgtcag cgagactttc
     9901 tcgagtcgcg cggcgattct cctgccggac gtcgacggcg gcctcgagca cgtcgatgcg
     9961 ggcgacgaag actcgcgatt cgcgtatccg gacctctcgg tcgcacagtg ggtgttcgac
    10021 cacggccgcg cggccggcct cggcaccgat gccctgcccg gcgcaccggc catctacctg
    10081 ccgctggcag ggacggaccg cacgcttggc gtcctggcgg tgcttccggc caacaatcgt
    10141 cgcgtgctgc ttcccgagca gaggcacctg ctcgagacgt tcgccagcca gatcgcgctg
    10201 gccatcgagc gcgccgtgct cggggaggcg gcacagtccg cgaagatcgc ggccgagacc
    10261 gaggcgctgc gcaacacgct cctggcatcg atctcccacg acctgcgaac cccgctggcc
    10321 gtgatcgcgg gcgccggttc tgcgctggcc gatccgaagc tcgcgctcga tcccgacgcc
    10381 cggacagcgc tggcacgcac gatcgccacc aagtcccagg aaatctccga cctcgtctcc
    10441 aacgtgctcg acctgatgcg attcgagtcg ggccacgttc ccctgcgcct ccagtgggag
    10501 acgctcgacg acctcgtcgg actcgcgctg ggaaaggttg agtcgcgact gggtcgacga
    10561 ccggtgcacg tcatgctgcc gccggagttg cccggggtga acgtcgacgc ggtgctcatc
    10621 acgcaggtgc tcgtcaatct cctcgagaac gccgcgaagc acacgcccga ggggacccac
    10681 gtgtggatca gcggtcacgc cgacggtgcc ggcgtccagg tcgtcgtcga ggacgacggg
    10741 ccgggacttc cgcccggcga ccccgacctc ctgttcgcca agttccagcg cggcaggacc
    10801 gagagcgagg tacccggagc gggcctcggc ctcgcgatct gccgcgcaat cgtcgaggcg
    10861 cacggtgggc acgtcacggc ctcgaatcgc gacggcggcg gcgcgcgatt cgtgctgacc
    10921 ctgcccgtcg aggcgacacg cgcgtgaccg acgccatgtt ccggctgctg gtcgtcgagg
    10981 acgaccgcga catccgcgaa ttgctgcgca cgatgctcga ggcccagggc taccgcgtcg
    11041 tcctcgccga gtcggccgcc cgcgcgctcg tggaagcacg atcgaacaag ccggacgcgc
    11101 tgctcgtgga cctcggattg ccggatcgcg acggacagga cctgatccgt gacgtccgcg
    11161 cattctcgac cgtgcccatc atcgtgctgt ccgcgcgcgc catggagtcg gagaaggtcc
    11221 tcgcgctgga cggtggcgcc gacgactacg tcaccaagcc cttccagtcg aacgagttgc
    11281 tggcccggat ccgcgcggcg ctgaggcgca gcgtgcgctc ggcggaccag tcacaggttc
    11341 tcgagatcgg agatgtcgtg atcaacctgg agacgcgcga ggcccagtcg ccgcgtggcg
    11401 cggtgcacct caccccggtc gagttccggt tgctggcgtg cctggcacga aacctggggc
    11461 tcgtcgtcac gcaggagcag gtcatccgcg acgtctgggg gccggaccgg gtcaccgaca
    11521 cgcgcgggct gcgctcgtac gtgaagacgc tgcgccagaa gctcgagccc gacccatcgc
    11581 ggccccgttt cctcctcacg gagccaggac ttggttaccg aatggtcgcg gactcgccgg
    11641 tcgcgccccg cacctgacga cggatccgta ccgggacgta cttgtggtag ggcgtccggg
    11701 tgaagggatc gcagtggctc gatgaggtca ggcgattcag cgccgggccg atcggtccgc
    11761 gtccgtcgaa ttgcccgccg tatccgtgcg gcagagtcac gacgcccggt cgcacgctgt
    11821 cgtcgatctc gatcgagacg tcgatttcgc cgctcgcgga ttgcacgacg gcacgatcgc
    11881 cattcacgac gccgatattg gcagcgtcgg ctggatgcac ccgcatggca ccgtccttgt
    11941 ccacctttcg ccacgccggg tcgcggaaga tctggttcgc gttgtagctg cggcgctcgc
    12001 ccgccatcag gatgaatgga aaggactcga ccgcgagcga ctcggacgac aggccgtcca
    12061 attcggccag catctccgga atcgcgagcc ggatgcgctt gtccggcgtg cgcacgagcg
    12121 accacacctc ctcgtagtcg tggcggctca ggaccacgcc ggagcgctgt tcgaggatcg
    12181 cctggaacaa ccggctcccg agcgtgaggc gaccaccgcg atgacccgca cgccggacgg
    12241 cgacttcatg tcgggacgcg tactgcatcg cgagcggcag gatgaaggcg gcggccgcgg
    12301 cgccgttcgg cagcgcagat cccagcgtac ggtaggcaat cgacgctgcg tacggcaccc
    12361 actgcttgtg ggtcgcgagg gtcgcggcca gcaacaccat gtaaggcagg tactgcgctg
    12421 catccggctc gaggcgagcc acgctcgaca acaccgggaa tcgtcgcgga atcgcgccca
    12481 tcttctcgag caggcgcgtg tagatctccg gttcgggcag tgtctcgccc agcggctcga
    12541 acaacggatg acgcaggtga aagtagttct cgggaaagtc caggttgaac ccggtcgcct
    12601 cccacttctc gaactgcgat gccgccggca gcacgtagtg cgcgagcttc gcggtctcgg
    12661 tcatcgccac gtccacgacg accagcagtt cgagtgaccc gaacgccttc tcgtacgctg
    12721 cagtgtcagc ccaggtcatg agcgggttgc agctgtccac gaagaccgcg cgcacgcgat
    12781 cctccccggc gagcaggatc tcgtcgggaa ggatgttcgg cgggaacacg ccggcgatcg
    12841 gatgcatccg atggtgggcc gttcgcggca ggggcttgcc ctcgatccgg acccgctcgt
    12901 ccgtgtgccc gaggatgggc agcaggtgcg tgtgcaggtt gttggtgcct gcgcgcccga
    12961 actggccggt gaccagccag agcagcttct cgagataggc gttgagtgtc gtgtgcagcg
    13021 tgtgctgcgt accgaggtcg acgcgcacgc aggccttgcg ggctgtcgcg aatccccggg
    13081 cgacgcgttc gacgtcagcc aacggcacgt ccgctcggcg cacgtactcc tcgatcggaa
    13141 cctcgcgcag gtggcgctcg accccgtcga atcccgagca gtgttgcgcc aggaatcccc
    13201 ggtcgatgag cccgtcgcgg accatgatcg cgagcatcgc tgcgaggagg tgcgcgtccg
    13261 ttccgggacg cagctgcagg tggatgtcgg cctgctccgc cgtctcggtg cggcggggat
    13321 cgacgacgac catcgtgcgc tgcgtgtcct tcctcaacgc gcgcagtgtg tcgcgcgcgc
    13381 tcgggattcc gtgcgactgg aacgggttgc agccgatgaa caggacgtag tccgcgtgct
    13441 cgacgtcctc ggtcgtgtgg caggtctggt gaccgaacag ccgaccgttg acccagaagt
    13501 caccggtctt ctcctggccg agcgagttgt aggcgaactt gctgcgcatc gcgggcagca
    13561 attgccggct gtaggcacca ccgaggtggt tgccctgccc cgccccgccg acgaacgcga
    13621 aggccttgcc gccctggcgg tcgcggatgt ccaccaggcg ccgcgcgatg tcgtcgagcg
    13681 cctcgtccca gccgacgcgc tcgaa
//
LOCUS       DAOMFD010000043        13549 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1088_length_13549_cov_30.207129, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000043 DAOMFD010000000
VERSION     DAOMFD010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13549
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1088_length_13549_cov_30.207129"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1797)
                     /gene="lepA"
                     /locus_tag="PL152_04145"
     CDS             complement(1..1797)
                     /gene="lepA"
                     /locus_tag="PL152_04145"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HQR48501.1"
                     /translation="MNHIRNFSIIAHVDHGKSTLADRFIQLCGGLEAREMQAQVLDSM
                     DLERERGITIKAQSVSLRYRSPDGQEYQLNLIDTPGHVDFSYEVSRSLYACEGALLVV
                     DAAQGVEAQSVANCYTAIEQGLEVVPVLNKIDLPSADADRVIKEIEEIIGIEAHDAVR
                     VSAKTGENVPALLEALVARIPPPSGDPDGPLQALIIDSWYDSYVGVVSLVRVVNGSLR
                     TGQKMRVWSTGRAYQVDKVGRFTPKSVAAEALYTGEVGFVIAGVKEIDGAPVGDTITL
                     ESKPCEAPLPGFKEIKPRVFAGLFPVRSDDYESFRDALQKLKLNDSALHYEPEVSTAL
                     GFGFRCGFLGLLHMDIVQERLEREYELELVTSAPTVIYEVATTGGEVLYVDNPAKLPP
                     PDKIAEVREPIIVANLLLPPDYVGPVMKLCLEKRGVQRKLQYLGSQVSMQWELPLAEV
                     VLDFFDRLKSVSRGYASFDYEFERFQAAPLVKLDILINGDKVDALSIITHRESAYQRG
                     RELVDKMRELIPRQMFEVAIQAAIGSHVIARSSVKALRKNVTAKCYGGDITRKRKLLE
                     KQKEGKKRMKQLGQVEIPQEAFLAVLQVGRKE"
     gene            complement(1840..2088)
                     /locus_tag="PL152_04150"
     CDS             complement(1840..2088)
                     /locus_tag="PL152_04150"
                     /inference="COORDINATES: protein motif:HMM:NF017574.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HQR48502.1"
                     /translation="MAEPGGNPVLYVRDGCHLCDQFLLELERDLGVASGRLRVVDVDG
                     DPGLAAEYGLRVPVLALDGQVVCEGIYDRPRVRHALRV"
     gene            complement(2136..3182)
                     /locus_tag="PL152_04155"
     CDS             complement(2136..3182)
                     /locus_tag="PL152_04155"
                     /inference="COORDINATES: protein motif:HMM:NF015825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucB/RseB C-terminal domain-containing protein"
                     /protein_id="HQR48503.1"
                     /translation="MRLACAALVGALGLVALPASAADGDDAQQWLERMSDALASRNYT
                     GVFTHSTRYQSETMRIVHRVGKGVTTERLVSLDGSGREIVRTAEEVHVYLPDRRVVLV
                     EPRTDRGVLLETLPEPGPELDALYTLQVSGGQKLLGRDVRIVDVRPRDAFRYGYRLWL
                     DEDSAMPLRTVVVDGAGHPVEVINFTLLELPHHIGARETEPALDSTGYQWVRSAPRPP
                     QRTVSAVVWRPMKLPPGFKLVANRTQVVPGVAFPVQHLIFSDGFASISVFIEPMRPEG
                     SAPPESSSVGSANAYSTQVHGRMVTAVGEVPLATVREVATSVAPTTVTTLVPMMPPAA
                     GPAEPADVAAASQR"
     gene            complement(3183..3764)
                     /locus_tag="PL152_04160"
     CDS             complement(3183..3764)
                     /locus_tag="PL152_04160"
                     /inference="COORDINATES: protein motif:HMM:NF015810.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor negative regulatory protein"
                     /protein_id="HQR48504.1"
                     /translation="MTDRIREQISALIDGELPEGEVGLLLRRLERDRQMRGAFGRYVL
                     AGEALRAPGGLLASSGFAARVSAAIDAGGGDAIPSQDTTSPRGARWNRPLLATAVAAS
                     AALMAVLLVVRGSHQAEMVAQSTLERARDGFASLPLSQAASPTPAHSQRLAGYLVAHS
                     QFASTIGRRTVWSSVLASDPGIARAAYESLEAP"
     gene            complement(3761..4420)
                     /gene="rpoE"
                     /locus_tag="PL152_04165"
     CDS             complement(3761..4420)
                     /gene="rpoE"
                     /locus_tag="PL152_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003252049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HQR48505.1"
                     /translation="MSNFPPSDGSIQSHEPGDDSSRPASGAEQSDLELVRRAQRGERG
                     AFDLLVLRYQHKVVKLVARLLRDRTEAEDVAQEAFVKAYRALGSFRGDSAFYTWLYRI
                     AVNTARNTLASRQRRPLEYEAGLSEAEQTAVEARMRHTDTPEATALSEEIHEAVNRAI
                     GELPEDLRTAIVLREVEGLSYEEIAAAMDCPVGTVRSRIFRAREAIDRVLEPLVDESR
                     A"
     gene            complement(4456..4875)
                     /locus_tag="PL152_04170"
     CDS             complement(4456..4875)
                     /locus_tag="PL152_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48506.1"
                     /translation="MKIEIRPWLLADRIAAGLAVGCALVIEAVAARYPEPQAGLGVAA
                     LALLAAWIWHGRRRARGLAGLSIDADGHWYTLYSDGRAVPSRVLPGTRLLGSTVALRW
                     QAAGEVRSAWLTPWDVPAGQLRELTVRISTTRDRAGA"
     gene            complement(4865..5062)
                     /locus_tag="PL152_04175"
     CDS             complement(4865..5062)
                     /locus_tag="PL152_04175"
                     /inference="COORDINATES: protein motif:HMM:NF015870.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase assembly factor 2"
                     /protein_id="HQR48507.1"
                     /translation="MRELDELLARYLEQEYGGASAAERGAFARILDLQDPQLFGYLLG
                     RDIPEEASLRHVVARIRRVED"
     gene            complement(5135..5917)
                     /locus_tag="PL152_04180"
     CDS             complement(5135..5917)
                     /locus_tag="PL152_04180"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HQR48508.1"
                     /translation="MPQFKLPANSRVRPGRSHPAPAGAQNTRRFRIYRFDPDSGENPR
                     VDSYEVDMDACGPMVLDALIKIKNEVDATLTFRRSCREGICGSCAMNIDGVNTLACTK
                     ATGEIRGDVKIYPLPHMPVVKDLVPDLTNFYAQYASVKPWLQTRTPAPPDSERLQSKE
                     DQEKIDRPSACILCACCSTSCPSYWWNSDRYLGPAALLAAYRWIIDTRDEATGERLDE
                     LEDPFRLYRCHTIMNCAEACPKDLNPARAIAEIKKLIAERQH"
     gene            complement(5919..7703)
                     /gene="sdhA"
                     /locus_tag="PL152_04185"
     CDS             complement(5919..7703)
                     /gene="sdhA"
                     /locus_tag="PL152_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HQR48509.1"
                     /translation="MSAYSFTDHTYDVVVVGAGGAGLRATFGLAEAGLSTACITKVFP
                     TRSHTVAAQGGISAALGNMGEDDWRFHFYDTIKGSDWLGDQDAIEYMTKEAPAAILEL
                     EHYGLPFSRTEDGKIYQRPFGGMTTHYGKGIAQRTCAAADRTGHAMLHTLYQQSLKHQ
                     ARFFVEYFAIDLVMEDGECRGVVALDMATGQLHRFRAHKVILATGGYGRAYFSCTSAH
                     TCTGDGGGMVLRAGLPLQDMEFVQFHPTGIYGAGCLITEGVRGEGGYLTNSRGERFME
                     RYAPNAKDLASRDVVSRSMTIEIREGRGVGPHKDHIHLHLEHLGAAVINERLPGIAET
                     SRIFAGVDVTREPIPVLPTVHYNMGGIPCNVHGEVVTLKDGNPDAVVPGLMAVGEAAC
                     VSVHGANRLGSNSLLDLVVFGRAAAHHCGKTVPRGSSHRPFRADAGELAVSRLDCFRN
                     AKGARRTAEIRLDMQKVMQNHAAVFRNGESLAEGVVKLRAVVDSFAEVQVTDRSLVWN
                     TDLVETLELDNLLGQAVATMHSAANRQESRGAHAREDYKDRDDQNWLKHTLAWVSGGT
                     NVRLDYRPVHLNTLTSDVESIPPKARTY"
     gene            complement(7700..8086)
                     /gene="sdhD"
                     /locus_tag="PL152_04190"
     CDS             complement(7700..8086)
                     /gene="sdhD"
                     /locus_tag="PL152_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HQR48510.1"
                     /translation="MSLRSPLGKALGHGSAKSGSAHWYAQRVTAVALVLLGLWFAASL
                     ACLGSASHEQVTAWLRSPATYVPAVLLLAVGAYHSAIGLRVVVEDYVGAKGLRAAVLL
                     VINFALVGAAVTGVLAVLRIALGVAP"
     gene            complement(8083..8478)
                     /gene="sdhC"
                     /locus_tag="PL152_04195"
     CDS             complement(8083..8478)
                     /gene="sdhC"
                     /locus_tag="PL152_04195"
                     /inference="COORDINATES: protein motif:HMM:TIGR02970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HQR48511.1"
                     /translation="MAQRERPLSPFMHYRWQYTNTLSILHRITGVLLSGGFFLLVYWL
                     AAAAAGPERYAAALTCLATPLMQFVMAGALFAFCYHLMMGLRHLAFDLGIGFELETAR
                     WTGWAAAGAAIALTCVLWIALHASLGGGA"
     gene            8818..9690
                     /locus_tag="PL152_04200"
     CDS             8818..9690
                     /locus_tag="PL152_04200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48512.1"
                     /translation="MTESTDPGPPAPLPFLGLLRAEGPDAASFLQGQLTNDVRLLADG
                     RTQLAALNTPQGRVVALLRLREQEGAVYALLPADLVDKVVTLLRRYVLRAKVRLEPAA
                     GVVMTWCDSAAPPGAPASSPITFQYAPGRIVVATPGPAAGIADVAAGPTHARSPQQDW
                     MAADIAAGLPQVFSATSEAFIPQMLNLDLLDGVSFSKGCYTGQEIVVRTQHLGRIKRR
                     TLRYRVASGPPPAPLDGLFHDGQKAADVLMSARVGDAVELLAVTQLEARHLSLALADG
                     RVATPVDLPYAVPA"
     gene            9687..>9936
                     /locus_tag="PL152_04205"
     CDS             9687..>9936
                     /locus_tag="PL152_04205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48513.1"
                     /translation="MSRRLPSAGTIASALVPVAAAGTLLAVAGLSIGPGLLATAVIML
                     VGAVIAAVHHAEVIAHRVGEPFGTLVLALAVTVIEASLI"
     assembly_gap    9937..10036
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10037..10930
                     /locus_tag="PL152_04210"
     CDS             <10037..10930
                     /locus_tag="PL152_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48514.1"
                     /translation="FGTLVLALAVTVIEASLIVSMMLSSIVDASALARDTVFATVMII
                     CNGVVGLCLLVGTLRHRIVDFRVEGTIPALAVLATLTTLTLVLPNFTVATAGPTLSKP
                     QLAFAGVISLVLYFVFVQVQTIRHRDYFLPVHSAPADTHAEPPPAAAAWASLALLLVA
                     LVAVVGLAKAVSPSIEAAVSAAGLPHAVVGICIALVVLMPETISAVRAARRARMQTSF
                     NLALGSALATIGLTIPVVAATSIALGLPLALGLAPTQIVFLALTLLLTFMTLSSGRAT
                     VLQGAVHLVLFATYLFLAAIP"
     gene            complement(10969..11856)
                     /locus_tag="PL152_04215"
     CDS             complement(10969..11856)
                     /locus_tag="PL152_04215"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQR48515.1"
                     /translation="MSSLQLFVACVSIWGTTWLAITFQLGRVPPEASVAYRFLLASVL
                     MFAYCRARGLALRLPARTHAWIALQGVFMFGASYVLVYYAEQRLASGLVAVGYSASPL
                     LGMVGQRVFLGTPMTARVAIGSLLGICGISLVFAPEISLTGAAAPSVLGIVFTLLAVV
                     FSMFGSVAAHRNQDAGIPLWQTMAFGMLYGAAFAAAVNLALGQPFAFATTIAYVSSLA
                     YLAVCGSILAFAAFLTLIGQIGAARASYVGVMTPVIALAVSAAFEGFEWQPLTWAGIA
                     VSLAGNVLILARSGSRMSR"
     gene            complement(11874..13379)
                     /gene="lysS"
                     /locus_tag="PL152_04220"
     CDS             complement(11874..13379)
                     /gene="lysS"
                     /locus_tag="PL152_04220"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011532564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HQR48516.1"
                     /translation="MSEERPGESTESEVNHLIAERREKLARLRERGVAFPNDFRRDST
                     AEELHVGYGAHPPEWFDAHPTQVAVAGRMMFKRVMGKASFAKLKDRSGMIQLFLQQDA
                     LAAAYEEFKGWDVGDILGAEGTLFKTKTGELSVRVGTLRLLVKSLRPLPDKWHGLADV
                     EQRYRQRYVDLIVSDESRQVFRARNQVVKFLRDFLDAMDFMEVETPMMQVIPGGAVAR
                     PFVTHHNALDVDMYLRIAPELYLKRLVVGGFERVYELNRNFRNEGLSTQHNPEFTMLE
                     LYQAYADYRDLMDMVERIFQGLADTIVGRRKVEYQGETIDLGSPFRRVTVEDLVVQHV
                     PNIDRARIRDLDYLRQVCARLGIAVHAADGPGKLQIELFEKTGEHTLRQPTFVYAYPA
                     EVSPLARRNDADPFITDRFEFFIGGRELANGFSELNDADDQAARFREQVARKSSGDEE
                     AMFYDADYVRALEYGLPPTAGLGIGVDRVVMLLTNQPSIRDVLLFPHMRPE"
     gene            complement(13391..>13549)
                     /gene="prfB"
                     /locus_tag="PL152_04225"
     CDS             complement(13391..>13549)
                     /gene="prfB"
                     /locus_tag="PL152_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_117295277.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HQR48517.1"
                     /translation="KSDVSWGNQIRSYVLDQSRIKDLRTGVEIGNTGAVLDGDLDPFL
                     EASLKQGL"
BASE COUNT         2070 a         4731 c         4530 g         2118 t
ORIGIN      
        1 tcattccttg cggccgacct gcagcacggc gaggaaggcc tcctgcggga tctcgacctg
       61 cccgagctgc ttcatgcgct tcttgccttc cttctgcttc tcgaggagct tgcgcttgcg
      121 ggtgatgtcg ccgccgtagc acttggccgt cacgttcttg cgcagcgcct tgacgctcga
      181 gcgcgcgatg acgtgcgagc cgatcgccgc ctggatggcc acctcgaaca tctggcgcgg
      241 gatgagctcc cgcatcttgt cgacgagttc ccggccgcgc tggtaggcgc tctcgcggtg
      301 cgtgatgatc gagagcgcgt cgaccttgtc gccgttgatg aggatgtcca gcttgacgag
      361 cggcgccgcc tggaagcgct cgaactcgta gtcgaatgac gcgtagccgc ggctcacgga
      421 cttgaggcgg tcgaagaagt cgagcaccac ctcggcgagc gggagttccc actgcatcga
      481 cacctggctg ccgaggtact ggagcttccg ctgcacgccg cgcttctcga ggcacagctt
      541 catcaccggg cccacgtaat ccggcggcag cagcaggttc gcgacgatga tgggctcgcg
      601 gacctcggcg atcttgtcgg gtggcggaag cttggccggg ttgtcgacgt agagcacctc
      661 gccaccggtc gtcgcgacct cgtagatcac cgtcggtgcg ctggtcacga gttcgagctc
      721 gtactcgcgc tcgaggcgct cctgcacgat gtccatgtgc agcagcccga ggaatccgca
      781 gcggaacccg aacccgagcg ccgtggagac ctcgggctcg tagtgcagcg ccgagtcgtt
      841 cagcttgagc ttctgcagcg cgtcgcggaa cgactcgtag tcgtcggaac gcaccgggaa
      901 caggccggcg aacacgcgcg gcttgatctc cttgaaaccc ggcagcggcg cctcgcaggg
      961 cttcgactcg agcgtgatcg tgtcgccgac cggcgcgccg tcgatttcct tcacgccggc
     1021 gatgacgaaa ccgacctcgc cggtgtagag cgcctcggcc gcgaccgact tgggcgtgaa
     1081 gcggccgacc ttgtcgacct ggtaggcccg gccggtcgac cagacgcgca tcttctggcc
     1141 ggtgcgcagc gaaccattca cgacccgcac cagcgagacg acgccgacgt agctgtcgta
     1201 ccaggagtcg atgatgagtg cctgcagcgg cccgtcgggg tcgccgctcg gcgggggaat
     1261 cctcgccacc agcgcctcga gcagcgccgg cacgttctcc ccggtcttgg cgctgacccg
     1321 cacggcgtcg tgggcctcga tgccgatgat ctcctcgatc tccttgatca cccgatcggc
     1381 gtccgccgac ggcaggtcga tcttgttcag gaccggcacg acctcgaggc cctgctcgat
     1441 cgccgtgtag cagttggcga cgctctgcgc ctcgacgccc tgggcggcat cgacgacgag
     1501 cagcgcgccc tcgcaggcgt acagcgaccg cgaaacctcg tacgagaagt cgacgtgccc
     1561 cggggtgtcg atcaggttca gctggtattc ctgcccgtcg ggcgaccggt agcgcagcga
     1621 cacgctctgc gccttgatgg tgatgccgcg ctcgcgctcg aggtccatcg agtcgaggac
     1681 ctgggcctgc atctcgcgcg cctcgaggcc gccgcacagc tggatgaagc ggtccgccag
     1741 cgtggacttg ccgtggtcga cgtgcgcgat gatggaaaag ttgcggatgt ggttcatcgg
     1801 tgcgggcatc ccggcccggc gccggaacaa agctggcgat tatacccgca gcgcgtgccg
     1861 gacccgcgga cggtcgtaga ttccctcgca gacgacctgc ccgtcgaggg cgagcaccgg
     1921 cacccgcagg ccatactcgg ctgcgaggcc gggatcgccg tcgacgtcca cgaccctgag
     1981 gcgcccggat gccacaccga gatcgcgctc gagttcgagc aggaactggt cgcacaggtg
     2041 acagccgtcc cgtacgtaca ggacggggtt gccccccggc tcagccacgc gtaccgccgg
     2101 ctgtcgacac cgcccggacg actgctccca tgccgtcagc gctgcgaggc tgcggcgacg
     2161 tcggctggtt ctgccgggcc cgccgcaggc ggcatcatcg ggacgagcgt cgtcaccgtg
     2221 gtcggcgcca cggatgtcgc gacctcgcgg acggtcgcga gcggcacctc gcccacggcg
     2281 gtcaccatgc ggccgtgcac ctgcgtcgag tatgcattgg ccgacccgac gctggacgac
     2341 tccggcggcg ccgagccttc cggccgcatc ggctcgatga acacggagat cgacgcgaag
     2401 ccgtccgaga agatcaggtg ctgcaccggg aaggcgacgc ccggcacgac ttgcgtgcga
     2461 ttcgccacca gcttgaatcc cggcggcagc ttcatcggcc gccacacgac ggcggacacc
     2521 gtgcgttgtg gcggtcgcgg cgcgctcctc acccactggt aacccgtgga gtcgagagcg
     2581 ggctccgtct cgcgcgcccc gatgtggtgc ggcaactcga gcagcgtgaa gttgatgacc
     2641 tcgacgggat ggcccgcgcc gtccaccacg accgtgcgca gcggcatcgc cgagtcttcg
     2701 tcgagccaca ggcggtagcc gtatcggaac gcgtcgcgcg gccgcacgtc cacgatccgc
     2761 acgtcgcggc caagcagctt ctgcccgccc gagacctgca gcgtgtagag cgcgtcgagc
     2821 tccgggcccg gctcgggcag cgtctcgagc agcacgccgc gatcggtgcg cggctcgacg
     2881 agcacgacgc gccggtcggg caggtacacg tggacttcct cggcggtgcg cacgatctcg
     2941 cggccgctgc cgtccaggga aacgagtcgt tccgtggtca cgcccttgcc gacccggtgc
     3001 acgatgcgca tcgtctcgga ctggtatcgc gtcgaatgcg tgaacacgcc ggtgtagttg
     3061 cgcgacgcca gggcgtcact catccgctcc agccactgct gggcgtcgtc gccgtccgcg
     3121 gcgctcgccg gcagcgcgac caggcccaat gcgccgacca gcgctgcgca ggccagccgc
     3181 actcagggag cctcgagcga ctcgtaagcc gcgcgcgcga tgccggggtc cgaggccagc
     3241 acgctcgacc agaccgtgcg ccggccgatg gtgctggcga actggctgtg cgccacgaga
     3301 tagccggcga ggcgctggct gtgcgccggg gtcgggctcg cggcctgcga caggggcagc
     3361 gacgcaaagc cgtcgcgggc gcgctcgagc gtcgattgcg cgaccatttc cgcctggtgc
     3421 gagccgcgca cgaccagcag cacggccatc agcgcggcgc tggccgcgac cgcggtcgca
     3481 agcaatggcc ggttccaccg cgcgccgcgc ggtgaggtcg tgtcctgcga cggtatggca
     3541 tcgccaccgc cggcatcgat cgccgcactg accctggccg cgaagcccga gctcgcgagc
     3601 agcccaccgg gggcgcgcag cgcctcgccg gccagcacgt aacggccgaa tgcaccgcgc
     3661 atctgccggt cgcgctccag cctgcgcagc agcaggccga cctcgccctc cggcaactcg
     3721 ccatcgatca gcgccgaaat ctgttcacgt atgcggtctg tcatgctcgc gactcgtcaa
     3781 ccaggggctc gaggacgcgg tcgatcgcct cgcgggcccg gaatattctc gaacgaaccg
     3841 tgccgaccgg acagtccatc gcggcggcga tttcctcgta actcaatcct tcgacctcgc
     3901 gcaggacgat ggccgtgcgc aggtcctcgg gcaactcgcc gatggcgcga ttcactgcct
     3961 cgtgaatctc ctcggacagt gccgtggcct ccggcgtgtc ggtgtggcgc atgcgcgcct
     4021 ccaccgccgt ctgttcggcc tcgctcaggc cggcctcgta ctcgagcggg cgtcgctgcc
     4081 gggacgcgag ggtgttgcgg gccgtgttca cggcgatccg gtacagccag gtgtagaagg
     4141 cgctgtcgcc gcggaacgaa cccagcgccc gataggcctt cacgaaggcc tcctgggcga
     4201 catcctcggc ttcggtccgg tcacgcaaca gccgcgccac gagcttcacg accttatgct
     4261 ggtacctgag caccagcagg tcgaacgccc ctcgttcgcc ccgctgggcc cgcctgacga
     4321 gctcgagatc gctctgctcc gcgccggaag ccggtcgcga ggagtcgtcc ccgggctcgt
     4381 gcgactggat agacccgtcg gaaggaggaa agttcgacat agtctcgccg cggcgcccgg
     4441 cagatgggga agccgtcatg cccctgcccg gtcgcgggtg gtggatatac gtactgtcaa
     4501 ctcgcgcagc tgcccggccg gcacgtccca aggcgtcagc caggccgatc gaacctcccc
     4561 cgcggcctgc caccgcaacg ctaccgtgga tccgagcagg cgtgtcccgg ggagcacccg
     4621 cgacggaacc gcccgcccgt cggaatacag ggtgtaccag tggccgtcgg cgtcgatgct
     4681 gaggccggcc agtcccctgg cgcgccgccg cccgtgccag atccaggccg caagcagggc
     4741 cagcgcggcg acaccgagtc ccgcctgggg ctcgggatac cgcgccgcga cagcctcgat
     4801 gaccagcgcg cacccgactg cgaggcccgc cgcgatgcgg tccgcgagca gccacggacg
     4861 gatctcaatc ttcaacgcgt cgtatccgcg caacgacatg gcgcagggag gcctcctcgg
     4921 ggatgtcgcg cccgagaaga tacccgaaca actgcgggtc ctgcaggtcg agaatgcgtg
     4981 cgaatgcacc gcgctctgcg gcagacgcgc ctccgtactc ctgctccaga taccgagcca
     5041 ggagctcgtc gagctcgcgc atgccacggc ggcagcgcca ccggagacgt gaatattcga
     5101 cctcttgtcc gaacgtcata tcactccgct ggcgtcagtg ctggcgctcg gcgatcagct
     5161 tcttgatctc ggcaatggcc cgcgccggat tcaggtcctt cgggcacgcc tccgcgcagt
     5221 tcatgatggt gtggcagcga tagagccgga acgggtcctc gagctcgtcg agccgctcgc
     5281 cggtggcctc gtcgcgcgtg tcgatgatcc agcgataggc cgcgagcagt gccgccgggc
     5341 cgaggtagcg gtcggagttc caccagtacg acgggcagct cgtcgagcag cacgcgcaga
     5401 ggatgcaggc cgacggccgg tcgatctttt cctggtcctc cttcgactgc agccgctcgc
     5461 tgtcgggcgg cgcgggcgtg cgcgtctgca gccacggctt caccgaggcg tactgcgcgt
     5521 agaagttggt gaggtcgggg acgaggtcct tgaccaccgg catgtggggc aacgggtaga
     5581 tcttcacgtc gccgcggatc tcgccggtcg ccttggtgca ggccagggta ttcacgccgt
     5641 cgatgttcat cgcgcacgag ccgcagatgc cctcgcggca cgaacggcgg aacgtgagcg
     5701 tcgcgtcgac ctcgttcttg atcttgatga gcgcgtccag gaccatcggg ccgcacgcgt
     5761 ccatgtcgac ctcgtagctg tccacgcggg ggttctcgcc cgagtcaggg tcgaatcggt
     5821 agatacgaaa gcgacgcgtg ttctgcgccc cggcgggcgc cgggtggctc ctgcccggac
     5881 gcacccggga attcgccggc agcttgaatt gcggcatgtc agtaggtcct ggccttcggc
     5941 gggatggatt ccacgtcgga ggtgagcgtg ttcaggtgca ccgggcgata gtcgaggcgc
     6001 acgttcgtgc caccactcac ccacgcgagc gtgtgcttga gccagttctg gtcgtcgcgg
     6061 tccttgtagt cctcgcgtgc gtgcgcgccg cggctttcct ggcgattcgc cgcggaatgc
     6121 atggtcgcga ccgcctgtcc cagcaggttg tccagttcca gcgtctcgac gaggtccgtg
     6181 ttccacacga gcgagcgatc cgtgacctgc acctcggcga agctgtcgac cacggcgcgc
     6241 agtttcacca cgccttccgc cagggactcg ccgttgcgga acaccgcagc gtggttctgc
     6301 atgaccttct gcatgtccag ccggatctcg gcggtgcgtc gcgcgccctt cgcgttccgg
     6361 aagcagtcga ggcggctgac cgcgagttcg cccgcatcgg cgcgaaacgg ccggtgcgag
     6421 gagccgcggg gcacggtctt tccgcagtgg tgtgccgcgg cacgcccgaa cacgacgaga
     6481 tcgagcagcg agttcgagcc gagccggttt gcgccgtgga ccgaaacgca ggccgcctcg
     6541 ccgactgcca tcagccccgg cacgacggca tcggggttcc cgtccttgag cgtgacgacc
     6601 tcgccgtgca cgttgcaggg gattccaccc atgttgtagt gcacggtcgg cagcaccggg
     6661 atcggctccc tggtcacgtc caccccggcg aagatgcgcg aggtctcggc gatgcccggc
     6721 aggcgctcgt tgatcacggc ggcgccgagg tgctcgaggt gcaggtggat gtggtccttg
     6781 tgcgggccca cgccgcgccc ttcgcggatc tcgatggtca tcgagcggct cacgacgtcg
     6841 cgcgacgcca ggtccttggc gttcggcgcg tagcgctcca tgaaccgttc gccccgcgag
     6901 ttggtgagat aaccgccctc gccgcgcacg ccctcggtga tcaggcagcc cgcgccgtag
     6961 atgccggtcg ggtggaactg cacgaactcc atgtcctgca gcggcaggcc cgcgcgcagc
     7021 accatgccgc cgccgtcccc ggtgcaggtg tgcgccgacg tgcaggagaa gtaggcacgg
     7081 ccgtagccac ccgtggcgag gatcaccttg tgggcacgga accggtgcaa ctgccccgtc
     7141 gccatgtcga gggccacgac tccccggcac tcgccgtcct ccatgaccag gtcgatggcg
     7201 aagtattcga cgaagaagcg cgcctggtgc ttgagcgatt gctggtacag cgtgtgcagc
     7261 atcgcatggc cggtccggtc ggccgcggcg caggtgcgct gcgcgatgcc cttgccgtag
     7321 tgcgtggtca tgccgccgaa ggggcgctgg tagatcttgc cgtcctcggt gcgcgagaac
     7381 ggcaggccgt agtgctcgag ctcgaggatg gcggccggcg cctccttggt catgtactcg
     7441 atcgcgtcct ggtcgccgag ccagtcggag cccttgatgg tgtcgtagaa gtggaagcgc
     7501 cagtcgtcct cgcccatgtt gccgagcgcg gcgctgatgc cgccctgcgc cgcgaccgtg
     7561 tggctgcgag tcgggaagac cttggtgatg caggcggtgg agagccccgc ctccgccagg
     7621 ccaaaggtcg cgcgcaggcc cgcaccgccc gcgccgacga ccacgacgtc gtaggtgtga
     7681 tccgtgaagc tgtaggcgct catggcgcga ctccgagcgc gatgcgcagc acggccagca
     7741 cgcccgtcac ggcggcgccg accagcgcga agttgatgac gagcagcacc gccgcgcgca
     7801 ggcctttcgc gccgacgtag tcctcgacga cgacccgcag gccgatcgcc gagtgatagg
     7861 cgcccacggc gaggagcagc accgccggca cgtatgtcgc cggcgaccgc agccatgccg
     7921 tgacctgctc atgggaagcg cttccgaggc atgccagcga cgctgcgaac cacagcccga
     7981 gcagcacgag cgcgacggcc gtgactcgct gggcgtacca gtgcgcggag ccgctcttag
     8041 ccgaaccgtg gcccagcgcc ttgccgagcg gactgcgcag gctcatgcgc cacccccgag
     8101 cgaggcatgc agcgcaatcc agaggacgca cgtgagcgca atcgccgccc cggcggcagc
     8161 ccatccggtc cagcgcgccg tctcgagctc gaacccgatc ccgaggtcga acgcgaggtg
     8221 cctcaagccc atcatgaggt ggtagcagaa tgcgaatagc gcaccggcca tcacgaactg
     8281 catcagtggc gtcgcgaggc aggtcagtgc cgcagcgtag cgctcgggac cggccgcggc
     8341 ggccgcgagc caatacacca gaaggaagaa accaccgctc agcagcacac cggtgatccg
     8401 gtgcaggatc gacagcgtat tggtgtactg ccaccgatag tgcatgaacg gggacaacgg
     8461 ccgttcgcgc tgggccatgc tcaattacct ccaggaccgg ccgctagaaa tcgatgcagc
     8521 ggcccgcttt ttcccagtcc ccgaaccgcg tcggttcggg gccctcccgg ccaccgattt
     8581 cccggggccg cgtcggatcg acgggctgcg gcgcagccga cgctggaggc aaggattcag
     8641 gctggggcgg gggcagaact tcgtgcggtg cattcatggt atcaaccccg aagtctgcca
     8701 gatccacctg ccgttcacca ctttgaaaac aaaggcaaat ttgcctgccg ggccgcggga
     8761 tggcacggta ggtcgcacgt tcccaacctc gccgagaccc ggatgaccgg ccctgccatg
     8821 actgaatcga ccgaccccgg cccgcccgcg ccattgcctt tcctgggact cctgcgcgcc
     8881 gaaggcccgg atgccgcgag cttcctgcag ggccagctga cgaacgacgt gcgcctgctc
     8941 gccgacggac ggacgcagct cgcggcgttg aacaccccgc agggtcgggt ggtcgcgctg
     9001 ctgcggctgc gcgagcagga aggcgctgtg tacgcgctgc tcccggccga cctcgtcgac
     9061 aaggtggtga cgctgctgcg ccgctacgtc ctgcgcgcga aggtgaggct ggaaccagcg
     9121 gcgggtgtgg tcatgacatg gtgcgactcg gccgccccgc ccggtgcgcc agcgtcgtcc
     9181 ccgatcacat tccaatacgc gccgggaaga atcgtcgtcg cgactcccgg tcccgccgcc
     9241 ggcattgcgg acgtcgccgc gggaccgacg catgcacgca gccctcaaca ggattggatg
     9301 gcggccgaca ttgccgcggg cctgccgcag gttttctcgg caacgtccga agcgttcatc
     9361 ccgcagatgc tgaacctcga cctgctcgac ggcgtgagct tctccaaggg ctgctacacg
     9421 ggacaggaaa tcgtcgttcg cacccagcac ctcggccgca tcaagcgccg cacgttgcgt
     9481 tatcgcgtgg cgtcgggccc gccgccggca ccgctcgacg gcctgttcca cgacggccag
     9541 aaggcggccg acgtcctcat gagcgctcgc gtcggcgacg ccgtggaact gctcgccgtc
     9601 acccagctcg aggcgcgtca cctgtccctg gcgctcgccg acggccgcgt cgccacgccg
     9661 gtggacctgc cgtacgcggt gccggcgtga gccggcgcct gccgtccgcc ggcacgattg
     9721 cctcggcgct ggtgccggtt gcggccgcag gtacgctgct cgccgtcgcc ggcctgtcga
     9781 taggaccggg cctgctcgcc acggccgtga tcatgctggt cggggcggtc attgcggccg
     9841 tccaccacgc ggaagtcatc gcgcaccggg tcggcgagcc gttcggcacg ctggtgctgg
     9901 cgctcgcagt gaccgtcatc gaggcgtcgc tcatcgnnnn nnnnnnnnnn nnnnnnnnnn
     9961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10021 nnnnnnnnnn nnnnnnttcg gcacgctggt gctggcgctc gcagtgaccg tcatcgaggc
    10081 gtcgctcatc gtctcgatga tgctgtcgag catcgtcgac gcgagcgcgc tcgcgcgcga
    10141 cacggtgttt gccaccgtca tgatcatctg caacggcgtc gtcggcctct gcctgctggt
    10201 cgggaccctg cgccaccgca tcgtggactt ccgcgtggaa ggcacgatcc ccgcgctcgc
    10261 cgtgctcgcg acgctgacca cgctcacgct ggtgctgcca aatttcaccg tggcgaccgc
    10321 cgggccgacc ctctcgaagc cgcagctggc cttcgcgggc gtgatctccc tggtgctgta
    10381 cttcgtcttc gtgcaggtgc agacgatacg gcaccgcgac tacttcctgc cggtgcactc
    10441 cgcgcccgcc gacacgcacg ccgaaccgcc gcccgccgcg gcggcctggg ccagcctggc
    10501 gctgctgctc gtggcactgg tcgccgtcgt cggcctcgcg aaagcagtct cgccgtccat
    10561 cgaggccgcc gtgtcggccg cgggactgcc ccatgccgtc gtcggcatct gcatcgcgct
    10621 ggtggtgttg atgccggaga cgatttccgc cgtgcgcgct gcgcgccgcg cccgcatgca
    10681 gaccagcttc aacctggcgc tgggatcggc actggcgacg atcggactca ccatacccgt
    10741 ggtggctgcc acctcgatcg cgctcggcct gccgctcgcg ctgggactcg cgccgaccca
    10801 gatcgtcttc ctcgccctca cgctgctgct gacgttcatg accctgagca gcggccgcgc
    10861 cacggtgctg cagggcgccg tgcacctggt gctgttcgcg acctacctgt tcctcgccgc
    10921 gattccctga cgcagcacct cgcccgcggt cgccggtgca ggttcacctc aacgcgacat
    10981 gcgggaaccg ctgcgggcga ggatcaacac gttgccggcg agcgaaaccg cgatgccggc
    11041 ccaggtgagc ggctgccact cgaatccctc gaacgcggcc gagaccgcga gcgcgatgac
    11101 cggcgtcatg acgccgacat agctcgcacg ggccgcgccg atctggccga tcagggtcag
    11161 gaacgcggca aacgccagga tcgaaccaca caccgcgaga tatgccagcg acgacacgta
    11221 cgcgatcgtc gtcgcgaatg cgaatggctg gccgagcgcg aggttcaccg ccgcggcgaa
    11281 agcggcgccg tagagcatgc cgaacgccat cgtctgccac agcgggatgc ctgcgtcctg
    11341 gttgcggtgg gccgcgacgc tcccgaacat cgagaacacc accgccagca gggtgaacac
    11401 gatgccgagc accgagggcg ccgcggcgcc cgtgagcgag atctccggcg cgaagaccag
    11461 tgagatgccg cagatgccga gcagcgaccc gatcgcgacg cgcgccgtca tcggcgtgcc
    11521 gaggaacacg cgctgtccca ccatgccgag cagcgggctc gccgaatagc ccaccgcgac
    11581 cagaccggac gccaggcgct gctcggcgta ataaaccagc acgtaactcg cgccgaacat
    11641 gaacaccccc tgcagggcga tccacgcgtg cgtccgggcc ggcagccgta gcgcgagccc
    11701 ccgcgcacgg cagtacgcga acatcagcac cgaggcgagc aggaaccggt acgcgacact
    11761 cgcctcgggc gggaccctcc cgagctggaa cgtgatggcg agccaggtcg tcccccagat
    11821 ggagacgcag gcgacgaaca gctgcaggct gctcacgcgg gcaggccagc ctgctactcg
    11881 ggcctcatgt gcggaaacag cagcacgtcc cggatcgacg gctggttcgt cagcagcatc
    11941 accacgcggt ccacgccgat ccccagcccc gccgtcggcg gcagcccgta ttcgagcgcg
    12001 cggacgtagt cggcgtcgta gaacatcgcc tcctcgtcac ccgacgactt gcgcgcgacc
    12061 tgctcgcgga agcgggccgc ctggtcgtcc gcgtcgttca gctcggagaa gccgttggcg
    12121 agttctcgcc cgccgatgaa gaactcgaag cgatcggtga tgaacggatc cgcgtcgttg
    12181 cggcgcgcga gcggcgagac ctcggccgga tacgcgtaga cgaacgtcgg ctggcgcagg
    12241 gtgtgctcgc cggtcttctc gaagagctcg atctgcagct tgccgggccc gtcggccgcg
    12301 tgcaccgcga tgcccagcct cgcgcagacc tgccgcaggt agtcgaggtc gcggatccgc
    12361 gcgcgatcga tgttcggtac gtgctgcacg accaggtctt ccaccgtcac gcggcggaac
    12421 gggctgccga ggtcgatcgt ctctccctgg tactcgacct tgcgcctccc gacgatcgtg
    12481 tcggccaggc cctggaagat gcgctcgacc atgtccatca ggtcgcggta gtccgcatag
    12541 gcctggtaga gctcgagcat ggtgaactcg ggattgtgct gcgtggacag tccctcgttg
    12601 cggaagttgc ggttgagctc gtagacccgc tcgaagccgc ccacgaccag gcgcttgagg
    12661 tacagctccg gcgcgatccg caggtacatg tccacgtcga gcgcgttgtg gtgcgtgacg
    12721 aacggccgcg caaccgcgcc gccggggatc acctgcatca tcggggtctc gacttccatg
    12781 aaatccatgg cgtcgaggaa gtcgcgcagg aatttcacga cctggttgcg cgcgcggaag
    12841 acctgccggc tctcgtcgct gacgatcagg tccacgtagc gctgacgata gcgctgctcg
    12901 acgtcggcca gcccgtgcca cttgtccgga agcggccgca gcgacttcac cagcagccgc
    12961 agcgtcccga cgcgcacgga gagttccccg gtcttcgtct tgaacagcgt cccttccgct
    13021 ccgaggatgt cgccgacgtc ccagcccttg aactcctcgt acgcggcggc cagcgcatcc
    13081 tgctgcagga acagctggat catgccgctg cggtccttga gcttggcgaa gctcgccttg
    13141 cccatgacgc gcttgaacat catgcggccc gcgacggcga cttgcgtcgg gtgcgcgtcg
    13201 aaccactcgg gcgggtgggc accgtagccg acgtgcagtt cctcggccgt cgagtcgcgg
    13261 cgaaagtcgt tcggaaacgc cacgccgcgt tcgcgcaggc gagcgagctt ctcgcgccgc
    13321 tccgcgatca ggtggttgac ctcgctctcg gtcgattcgc ccggcctttc ctctgacatg
    13381 ccgccgctcc tcacagtccc tgcttcaggc tcgcctcgag gaacgggtcg aggtcgccgt
    13441 cgagcaccgc gcccgtgttg ccgatctcga cgccggtacg caggtccttg atgcgcgact
    13501 ggtcgagcac gtaggaacgg atctggttgc cccagctcac gtccgactt
//
LOCUS       DAOMFD010000044        13541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1090_length_13541_cov_32.565030, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000044 DAOMFD010000000
VERSION     DAOMFD010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13541
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1090_length_13541_cov_32.565030"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="PL152_04230"
     CDS             <1..786
                     /locus_tag="PL152_04230"
                     /inference="COORDINATES: protein motif:HMM:NF018657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT domain-containing protein"
                     /protein_id="HQR48518.1"
                     /translation="FKAIRHHVGDVTINDQFLCIVGGALRKYLQSKRELPAASMAAMV
                     PMTLRGADKGGDVGNQVGFTVMQVRTDIADPVKRLLAIRDGAQEAKRLTDAIGREFAR
                     DLMEFVPNVVSQVVARYARPPRIGLIISNVRGPDVPLYMAGARLVNYSPISIPTHGLG
                     LNVTGFSYAGTMWICAVSCRDMLPDPAFFANCMRESFEEMKAAAARISTVEHAKALPQ
                     LPGRKATAGRKPAAGASHGAPAGTKRRKATRAKGKRRTTRKAA"
     gene            783..1184
                     /locus_tag="PL152_04235"
     CDS             783..1184
                     /locus_tag="PL152_04235"
                     /inference="COORDINATES: protein motif:HMM:NF025078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4157 domain-containing protein"
                     /protein_id="HQR48519.1"
                     /translation="MIGASAAACEMPRTRDLLPVAQRAALERLLGTSVDEVEVVERSW
                     YARMHPGARATTRRNRILLRGSAQDFVADPMLMLHEYYHVLRQWNRGSLTAWRYLAEW
                     LRRGYWQNRYERHARRFARTRLEAFQRLSRG"
     gene            1240..3165
                     /gene="mnmG"
                     /locus_tag="PL152_04240"
     CDS             1240..3165
                     /gene="mnmG"
                     /locus_tag="PL152_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002098 - tRNA wobble uridine modification
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HQR48520.1"
                     /translation="MRFTETFDVIVVGGGHAGTEAALAAARMGCRTLLVTQSIESLGQ
                     MSCNPAIGGIGKGHLVREIDALGGAMARATDRAGIQFRTLNASKGPAVRATRAQADRQ
                     LYKRAIRSLLENQPNLSLFQQEVSDLRIEGDRVTGVVTQVGVAVAARAVVLTVGTFLG
                     GRIHVGLDQYAGGRAGDPPSNRLAEKLRALPLRVGRLKTGTPPRIDGRTIDYRGLTVQ
                     HGDEPRPVFSFLGRRDEHPRQVPCHITATNARTHEIIRAGTDRSPMFTGVIEGVGPRY
                     CPSVEDKVVRFADRDSHHVFIEPEGLDTHEVYPNGISTSLPFDVQVEFVRTIRGFEQA
                     RITRPGYAIEYDFFDPRDLKATLETKCLRGLFFAGQINGTTGYEEAAAQGLIAGANAA
                     LAVLDREPWWPRRGDAYIGVLIDDLITRGAPEPYRMFTSRAEYRLTLREDNADLRLTT
                     VGRDLGLVDDERWEFFERKRDAVAQEQARLQAAWIRPGTDEAVRAESTIGPLVRELRA
                     FDLLRRPEVSYERVADIVGGADGDWRHDERLAAQVLLQVDVQGKYAGYIDRQHDEIER
                     QRQHEDTVLPADLDFAGVRGLSNEVRQRLTERRPATLGQAARIPGVTPAAVSLLLIHL
                     KRRTAAKPQPARARRGA"
     gene            3162..4844
                     /locus_tag="PL152_04245"
     CDS             3162..4844
                     /locus_tag="PL152_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HQR48521.1"
                     /translation="MTTIMMHVGSTWTNRSGVIGCTARVLVIGALVALAACSGGNEKS
                     KASEAPAAKARTVLTRGNGPEPDTLDPQRARTDGSFNILRDAFEGLTAVGPDGNAVPA
                     AADSWTVSSDGLEYRFHLRDGLRWSNGDRLVASDYVAGMRRLVDPTTASPYAQMLDPV
                     LNAPAITRGERPPADLGVSAPDDRTVVIRLKDPAPYLAGLLAEPGAFPVHGPSLAKFG
                     ASYARPGNLVSNGAFTLQDWVVGSRVDLVRNRNYWNDAATRIEGVRYLHHDDPTAEFR
                     QFRAGELDVTYVVPPQQFAWIKQNLPGALHISPQLSVYYYGFNLTRPPFKDQPGLRRA
                     LSMVIDRDRLVSAVTGLGEAAAYGWVPGGVANYTPQRFDYASRPYAERVAEARELYRK
                     AGYSESRPLEVEIRYNTGEVHNRLAVAVAAMWKEALGVKTTLYAEEFKALLQSIQARK
                     DTQVFRSSWVGDYNDAFTFLQLLQSRFGINMTGYANPRYDALLAEATRQADPARRREL
                     LQQAEAVMLADTPVLPLYFYVNKHLVQPYVLGWHDNVMNVQYSKDLGLEPAN"
     gene            4943..>5178
                     /locus_tag="PL152_04250"
     CDS             4943..>5178
                     /locus_tag="PL152_04250"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQR48522.1"
                     /translation="MAWFALFAATIFGLYFWREYRIQQRDLESASAPHRVGQRYIGQV
                     LTLSDRIEEGAGRMKLNGRRWQLRGPDLPAGAKVR"
     assembly_gap    5179..5278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5279..5954)
                     /locus_tag="PL152_04255"
     CDS             complement(<5279..5954)
                     /locus_tag="PL152_04255"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HQR48523.1"
                     /translation="MSLRHLVVPGMLAVLLTTAGPALADEARPRTVSVSGQGEVSAQP
                     DMARVTLGVEARRPTLSDARSRVTATVDRVLALTRDLKIDPKYVNATRLQVQPEYRWN
                     ETDRTRVLLGYVVSRQVEIELRDLDQLGALLERAVSAGANQVGDPSLDSTKRKDLERQ
                     AMALAVQDARLNAEALAQAAGVRLGAVRALNASGSSPPMPMYRMAAVAADAAAPPPEA
                     TYQASEM"
     gene            6165..6794
                     /locus_tag="PL152_04260"
     CDS             6165..6794
                     /locus_tag="PL152_04260"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR48524.1"
                     /translation="MTSRSAWLALSLTIAALVAPGLAGAADVARGGKLSYTCQGCHGI
                     PNYRNAYPVYSVPKLGGQHTAYMVAALKEYASGDRPHPTMHAQAITMAPQDMQDIVAY
                     LSGQELKPTGHAVGTAPKAGQTCVACHGIDGVGIMPEYPNLAGQHADYIEQALKAYRA
                     GQRKNPIMAGMAAPLKDAEIKELAAYYSSQRPGLCSTDETRKSGKCAGP"
     gene            6811..>7163
                     /locus_tag="PL152_04265"
     CDS             6811..>7163
                     /locus_tag="PL152_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR48525.1"
                     /translation="MTTPVGEGVYLIDTVYSRPGLAASHLVVDSGRAAFVDTGPAPGV
                     PRLLAALEEVGVGRDAVDYLFLTHVHLDHAGGAGQLMAALPNARAVLHPRGAPHLVEP
                     SKLIAGSIKVYGEQL"
     assembly_gap    7164..7263
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7264..7898
                     /locus_tag="PL152_04270"
     CDS             <7264..7898
                     /locus_tag="PL152_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR48526.1"
                     /translation="EPSKLIAGSIKVYGEQLYRELYGEIIPIPADRVLVTEDRQRIVL
                     GRRTFEFIDAPGHARHHHCPIDLDHCAVYSGDNFGICYRELDTAAGPLMLPTTTPVQF
                     EPDVLHKTIDRLMSYRPVRVYQTHFGPVDDLARLAADLHASIDELVRIARTHARAADR
                     RARIEADMYAYFDARLDAHGCRLSQAERHRLIDDDVRLNTAGLEVWLDRS"
     gene            7909..8898
                     /locus_tag="PL152_04275"
     CDS             7909..8898
                     /locus_tag="PL152_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HQR48527.1"
                     /translation="MPTFRAFRIHEIDRRIVARFDDVSLDDLSPGDVVIRVTHSDINY
                     KDALAATGKGRILRRYPLVGGIDLAGEVVSSTDARYVAGQKVLVTGCGLSETHDGGYA
                     EYARVHGDWVIPVPAGMSTLDSMKIGTAGFTAALAVHRMEQNGQQPGNGPVVVTGATG
                     GVGSIAISMLAGRGYEVVAVSGKPAADEYLRAIGATRILHRQDLDLGSKPMEKALWAG
                     AVDNLGGPVLTWLTRTTDQWGNIASVGLAGGAELATTVMPFILRGVNLLGINSVLTPR
                     ELRLEVWRRIATDLRPAALDRICTKAVRFDELPAQFDAYIEGRVTGRSVVTIP"
     gene            complement(8911..9489)
                     /locus_tag="PL152_04280"
     CDS             complement(8911..9489)
                     /locus_tag="PL152_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48528.1"
                     /translation="MNTRNPRSMARPATVGVLILAIAAGLPAHAGDTARQGAPAAAAP
                     LGAALPGGTIVSAKFVQITPAAGGTVTTATIAADGSFRATGLKPGSYKLAFTSTPKQT
                     QGATFGEKVQSGLAQTGNAVASGAARSAQAAPASPTAEGARRIDSTPARISTNFTVGK
                     QTGRAFVDGPAVDVEVGSDGVLAGRATPAAAD"
     gene            9916..11715
                     /locus_tag="PL152_04285"
     CDS             9916..11715
                     /locus_tag="PL152_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48529.1"
                     /translation="MSLFAELKRRNVFRVAAAYLALGWIVTQVTSTVAPMLHLPEWVG
                     PLVLWIGVIGFPFVAMFSWIYELTPEGIRKETEVDRTQSITHQTARRLDYVIIALLVV
                     AIALFAWSQFRPVARERLTVVATPQSATPGAGAVDRSVAVLPFVDMSAAKNREYFADG
                     ISEELLNLLAKVPQLRVIARTSSFSFKGKEVDIAEIAKKLNVANVLEGSVRTSGDKVR
                     ITAQLIRASDSSHLWSETYDRKMTDVFSVQDEIAAAVVAQLKVTLLGAAPSARKTDPR
                     AYALYLQGRELEFQYSTASLEQAVALYKQAVAIDPSYAPAWVHLTWAYFSQVDLGVLT
                     PAQGLERSRDALGKALAADPANAFAYATMALWEGVLERKYEVAARHVEQALALDPTNF
                     EAIDIAAVLARRLGRLDLAIALGEYLVVRDPVNPANHDDLALAYGYNGQLDKAIAARR
                     TVLSLSPDAGWQHTALGDLLLLKGDATAALAEYQKEPIECFHLVGLTKAYHALGRKAD
                     SDAARAGMMHKCVDKKPFNVAATLAYLGDVDGAFQMLDKAAQIQDTDLGAVAIFPAFA
                     GMHKDPRWLPLLRRLGLAPDQLAAIKLDVKVPQ"
     gene            11720..12541
                     /locus_tag="PL152_04290"
     CDS             11720..12541
                     /locus_tag="PL152_04290"
                     /inference="COORDINATES: protein motif:HMM:NF024946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HQR48530.1"
                     /translation="MRGPSGTWRNRRGGAAGRTRRLVAARATVAWLPLVGTLAHADGP
                     AFDRPGIAFSSTTMPAHSVTWEQGLLDFQRDQSGGVTQDAYVVDARLRYGLADRWEVQ
                     LAAPLYDEIDTHGSGQGLTTAGVGDLSLAVKLALTPRDDRFTMSMLGVVSFPTGNEDI
                     GAGAEQYSLGATLGWTLSDSQSLAFYTNVDLLAGDATWTFAPSWNFDLGETVGGYVEA
                     GYQPAAHGDPANALAGAGLMWLVKPTVQLDAYFLKGLTSESTDLAAGCGVSIFFP"
     gene            complement(12551..13009)
                     /locus_tag="PL152_04295"
     CDS             complement(12551..13009)
                     /locus_tag="PL152_04295"
                     /inference="COORDINATES: protein motif:HMM:NF013722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HQR48531.1"
                     /translation="MRYFEDFEVGSSQTAGVHELTAEDIIAFAREWDPQPWHVDPGAA
                     ERSPMRGLTASSVHTYAVAARLLSRMDPAAGIASIKHELELPNPARPGDRLTFTMTCV
                     EKRPSESKPDRGLITFEGVLANQHGTPVLKVRSLMMVKTRPPGRAPAPLE"
     gene            complement(13021..>13541)
                     /locus_tag="PL152_04300"
     CDS             complement(13021..>13541)
                     /locus_tag="PL152_04300"
                     /inference="COORDINATES: protein motif:HMM:NF021382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2083 domain-containing protein"
                     /protein_id="HQR48532.1"
                     /translation="RYNASFEQVSHRLSTLQRPGARGVPFYFVRVDAAGNITKRHSST
                     RFQFARFGGACPLWNVHEAFGQPGRILVQVAEMPDGVRYLCIGRSIVKRSGHYLRPNR
                     QYAIGIGCEIAHAEKVVYSEGLQLDGAAVPVGVSCRICERPDCHQRAFPPLGRRLTVR
                     PNERHVVPFSIE"
BASE COUNT         2155 a         4582 c         4580 g         2024 t
ORIGIN      
        1 ttcaaggcga tccgccacca cgtgggcgac gtcacgatca acgaccagtt cctgtgcatc
       61 gtgggcggcg cgctgcgcaa gtacctgcag agcaagcgcg agctgcccgc ggcgtcgatg
      121 gccgcgatgg tgcccatgac gctgcgcggc gcggacaagg gcggcgacgt cggcaaccag
      181 gtcggcttca cggtgatgca ggtgcgcacc gacatcgccg acccggtgaa gcgcctgctg
      241 gcgatccgcg acggcgcaca ggaagccaag cgcctgaccg acgccatcgg ccgggaattc
      301 gcgcgcgacc tcatggaatt cgtgccgaac gtcgtgtcgc aggtcgtcgc ccgctacgcc
      361 cggccgcccc gcatcgggct gatcatctcg aacgtgcgcg ggccggacgt gccgctctac
      421 atggccggcg cgcgcctggt gaactactcc ccgatcagca tcccgaccca cgggctgggc
      481 ctcaacgtca cggggttcag ttacgcgggc acgatgtgga tctgcgccgt gtcctgccgc
      541 gacatgctgc ccgacccggc cttcttcgcc aactgcatgc gcgagagctt cgaggagatg
      601 aaggccgccg ctgcgcggat cagcaccgtg gagcacgcga aggcgttgcc ccagctgccc
      661 gggcgcaagg cgacagctgg acgcaagccg gcggcgggtg cgtcgcacgg agcacccgcc
      721 ggcaccaagc gcagaaaggc gactcgcgcc aagggcaagc gtcgcacgac ccgcaaggcc
      781 gcgtgatcgg cgcaagcgcg gccgcctgcg agatgccgcg tacgcgtgat ctcctgccgg
      841 tggctcaacg cgccgcgctc gagcgcctgc tggggacctc cgtcgacgaa gtcgaggtgg
      901 tcgagcgatc gtggtacgcg cgcatgcacc cgggcgcgcg ggcgacgacc cggcgcaacc
      961 ggatcctgct caggggttcg gcgcaggact tcgttgcgga cccgatgctc atgttgcacg
     1021 agtactacca cgtgctccgc cagtggaacc gtggaagcct gacggcgtgg cgctacctgg
     1081 ccgagtggct gcgacgcggg tactggcaga accgctacga gcggcacgca cggcggttcg
     1141 ccaggacgcg gctcgaggca ttccagcggt tgagccgggg ctgaagcgcc gcgattcgcg
     1201 gcgaatccgt acaatgtgcg ccgcgcgagc gggcaaccca tgcgattcac ggaaacattc
     1261 gacgtcatcg tggtcggcgg cggccacgcc ggaaccgagg cggcactggc cgccgcgcgg
     1321 atgggctgcc gcacgctgct ggtgacgcag agcatcgaat cgctgggcca gatgagctgc
     1381 aacccggcga tcggcggcat cggcaagggc cacctggtgc gggaaatcga cgccctcggc
     1441 ggcgccatgg cccgcgcgac cgaccgcgcc ggcatccagt tccgcacgct gaacgccagc
     1501 aagggtccgg ccgtgcgcgc gacccgggcg caggcggacc ggcagctcta caagcgcgcc
     1561 atccgcagtc tgctcgagaa ccagccgaac ctgagcctgt tccagcagga agtgtcggac
     1621 ctgcgcatcg agggcgaccg cgtcaccggg gtggtgacgc aggtgggtgt ggcggtcgcg
     1681 gcgcgcgcgg tggtgctgac cgtcggcaca ttcctcggcg ggcgcatcca cgtcggcctc
     1741 gaccagtacg ccggcggccg tgccggcgac ccgccgtcga atcggctcgc cgaaaagctg
     1801 cgcgcgctcc cgcttcgcgt cggtcggctc aagacgggca cgccgccgcg catcgacggg
     1861 cgcacgatcg actaccgcgg cctcaccgtg cagcacggcg acgaaccgcg gccggtgttc
     1921 tcgttcctcg gacgacgcga cgagcatccg cggcaggtgc cctgccacat cacggcgacc
     1981 aacgcacgca cgcacgagat catccgcgcg ggcaccgacc gctcgccgat gttcacgggc
     2041 gtgatcgaag gcgtgggccc gcgctattgc ccgtcggtgg aggacaaggt cgtgcgcttc
     2101 gccgaccgcg attcgcacca cgtcttcatc gaacccgaag gcctcgacac ccacgaggtc
     2161 tacccgaacg gcatctcgac cagcctgccg ttcgacgtgc aggtcgagtt cgtgcggacg
     2221 atccgcggct tcgagcaggc gcggatcacg cggccgggct atgccatcga atacgacttc
     2281 ttcgacccgc gcgacctcaa ggccacgctg gagacgaagt gtcttcgagg actgtttttc
     2341 gcggggcaga tcaacggcac gaccggatac gaggaagccg cggcacaagg actcatcgcc
     2401 ggcgcgaacg ccgcgctggc ggtgctcgac cgcgagccgt ggtggcctcg ccgcggcgat
     2461 gcatacatcg gcgtgctgat cgacgacctg atcacgcgcg gcgcgcccga gccgtaccgc
     2521 atgttcacga gccgcgccga ataccggctg acgctgcgcg aggacaacgc ggacctgcgc
     2581 ctcacgacag tgggtcgtga cctcgggctg gtcgacgacg agcgctggga gttcttcgag
     2641 cgcaagcgcg acgcggtcgc gcaagagcag gcgcggttgc aggcggcgtg gatccgtcct
     2701 ggcacggacg aggcggtgcg cgcggagtcg accatcgggc cgctggtccg cgagctgcgt
     2761 gcgttcgatc tgctgcgccg gccggaagtg tcctatgagc gggtggccga catcgtcggc
     2821 ggggcggacg gtgattggcg ccacgacgaa cgtctcgccg cgcaggtcct gctgcaagtg
     2881 gacgtgcaag gcaagtacgc tggctacatc gaccgccagc acgacgaaat cgagcgccag
     2941 cgacaacatg aggatacggt cctgccggct gacctcgatt tcgccggcgt gcgcggcctg
     3001 tcgaacgagg tccgccagcg actcacggaa cgccgtcccg cgacgctcgg ccaggccgcg
     3061 cggattccag gcgtgacccc ggcggccgtg tcgctcctgc tcattcactt gaagcgccgg
     3121 actgcggcga agccacagcc agctcgcgcg cggcgtggag cgtgacaacg atcatgatgc
     3181 acgtcgggtc cacatggacg aatcgctccg gagtcatcgg ttgcaccgct cgtgtcctcg
     3241 tcatcggcgc actcgtcgcg ctggcggcct gcagcggcgg caacgagaag tcgaaggcat
     3301 cggaagcgcc ggccgccaag gcccgcacgg tgctcaccag aggcaacggc ccggaacccg
     3361 ataccctcga tccgcagagg gcgcgcaccg acggctcctt caacatcctg cgcgacgcct
     3421 tcgagggact gactgccgta gggccggacg gcaacgccgt tccggccgcc gcggattcct
     3481 ggacggtgtc gagcgacggc ctcgaatacc gcttccacct gcgcgacggg ctgcgctggt
     3541 cgaacggcga ccggctcgtc gcgagcgact acgtggccgg catgcgcagg ctcgtcgatc
     3601 ccacgaccgc gtccccgtac gcgcagatgc tggacccggt gctgaatgcg ccggccatca
     3661 cgcgcggcga gcggccgccc gcggacctcg gtgtcagcgc gccggacgac cgaacggtcg
     3721 tgattcggct caaggacccg gcgccctacc tggccgggct gctcgcggag ccgggcgcgt
     3781 tcccggtgca cggcccgtcc ctcgcgaaat tcggcgcgtc ttatgcgagg ccgggcaacc
     3841 tggtgtcgaa cggagcgttc acgctgcagg actgggtcgt gggctcgcgc gtcgatctcg
     3901 tgcgcaaccg caactactgg aacgacgcgg cgacccggat cgagggcgtg cgctacctgc
     3961 atcacgacga cccgaccgcg gaattccgcc aattccgcgc gggcgaactc gacgtcacct
     4021 acgtcgtgcc cccgcagcag ttcgcgtgga tcaagcagaa cctgcccgga gcgctgcaca
     4081 tctccccgca gttgagcgtc tactactacg gcttcaacct cacccgtccg ccattcaagg
     4141 accagcccgg cctgcggcgc gccctgtcga tggtgatcga ccgcgaccgg ctggtctcgg
     4201 cagtgaccgg gctcggggag gcggcggcct atggctgggt gccgggcgga gtcgcgaact
     4261 acacgccgca gcggttcgac tatgcgagcc gcccctatgc ggaacgggtc gccgaggccc
     4321 gcgagctgta ccgcaaggcc ggctattccg agtcgcggcc gctcgaggtc gagatccgct
     4381 acaacacggg cgaggtgcac aaccgcctgg ccgtggcagt ggcggcgatg tggaaggagg
     4441 ccctcggcgt caagacgacg ctctatgccg aggaattcaa ggcgctcctg cagagcatcc
     4501 aggcgcgcaa ggacacgcag gtcttccggt ccagctgggt cggcgactac aacgacgcgt
     4561 tcaccttcct gcagctgctg caatcccggt tcggcatcaa catgacgggc tatgcgaacc
     4621 cgcggtacga cgcgctgctc gcggaggcga cgcgccaggc cgaccccgcg cgccgccgcg
     4681 agctgctgca gcaggccgag gcggtgatgc tggcggacac gccggtcctg ccgctgtact
     4741 tctacgtcaa caagcacctc gtgcagccct acgtgctggg ctggcacgac aacgtcatga
     4801 acgtccagta cagcaaggac ctcgggctcg agccggcgaa ctgagcgcgg cgtcacagaa
     4861 tgcggtctgc cgcggggtgt ccgagttgag caccactgac aacttttggc acgatcacca
     4921 gacctgaaaa caagcgaacg ccatggcctg gtttgctctc ttcgccgcga cgatcttcgg
     4981 gctctacttc tggcgcgagt accgcatcca gcagcgggac ctcgagagcg catctgcacc
     5041 ccaccgggtc ggccagcgct acatcggcca ggtgctgacg ctgagcgacc gcatcgagga
     5101 aggcgcggga cggatgaagc tgaacggtcg ccgctggcag ctgcgcggtc ccgacctgcc
     5161 cgccggcgcc aaggttcgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc
     5281 atctcgcttg cctggtaggt cgcttccggt ggtggcgcgg ccgcgtccgc agcgactgcg
     5341 gccatccggt acatcggcat gggaggcgac gaaccggaag cgttgagcgc gcggaccgcc
     5401 cccagccgaa cgccggccgc ctgcgccagc gcctcggcgt tgagccgcgc gtcctggacc
     5461 gcgagggcca tggcctgccg ctccaggtcc tttcgcttcg tcgaatcgag cgacgggtct
     5521 ccaacctggt tcgcgccggc gctgacggcg cgttcgagca gcgcgccgag ctggtcgaga
     5581 tccctgagtt cgatctcgac ctggcgactc actacgtaac caagcagcac ccgggtccgg
     5641 tccgtctcgt tccagcggta ttccggctgg acctgcagcc tcgtcgcatt gacgtacttc
     5701 gggtcgatct tgaggtcgcg cgtgagggcg agcacccggt ccaccgtggc ggtgacccgc
     5761 gatctcgcgt ccgacagggt cggccgccgc gcctcgacac ccagcgtcac ccgcgccatg
     5821 tccggctggg ccgagacctc gccctggccc gagacgctca ccgtccgcgg gcgggcctcg
     5881 tcggccagtg ccggcccggc ggtcgtcaac aggacagcca gcattccggg cacgacgaga
     5941 tggcgcaggg acatggttcg ctcctggggt gacggacggg aactcgcgat caggggagta
     6001 tgacagcgac cgcgtgccac cgtgccgcgg tactcgcgcc cacgccaggg acgcggcagg
     6061 ccgaatggcg tctccgccgc tggctcattc gactcacgaa acgcttttga ttaacatgcc
     6121 gccccttcgg cccgggggag gccggcttcc gcatggagtt tgacatgacc tcgagatcgg
     6181 cctggctcgc gctgagcctg accatcgcgg cgctcgtcgc gccggggctc gccggggcgg
     6241 cggacgtcgc ccgtggcggc aagttgtcct acacctgcca gggctgccac ggcatcccga
     6301 actaccggaa tgcctatccc gtctacagcg tcccgaagct gggcggccag cacacggcgt
     6361 acatggtcgc ggccctcaag gagtacgcgt ccggggaccg gccgcacccg acgatgcacg
     6421 cgcaggccat cacgatggcg ccgcaggaca tgcaggacat cgttgcgtac ctgagtggcc
     6481 aggaactcaa gcccacggga cacgcggtcg gcacggcccc caaggcggga cagacctgcg
     6541 tcgcctgcca cggcatcgac ggcgtgggca tcatgcccga gtacccgaac ctcgccgggc
     6601 agcacgccga ctacatcgag caggccctca aggcctaccg cgccggccag cgcaagaacc
     6661 cgatcatggc gggcatggcc gcgccgctga aggatgcgga gatcaaggaa ctggccgcgt
     6721 actactcgag ccagcggccg ggactgtgct cgaccgacga gacccgcaag tcgggcaagt
     6781 gcgcaggacc ctgagccggc cgacgcccgg atgaccaccc cggtcggcga gggcgtgtac
     6841 ctgatcgata cggtgtactc gcgccccggc ctggcagcga gccacctggt cgtcgattcg
     6901 ggtcgcgcgg cattcgtgga caccgggccc gcgccgggcg tgccgcgact cctcgccgcg
     6961 ctggaggaag tcggtgttgg gcgagacgcg gtcgactatc tcttcctcac gcacgtgcac
     7021 ctcgaccacg cgggcggggc cggccagctc atggcggcgc tgccgaacgc gcgtgccgtg
     7081 ctgcacccgc gcggcgcgcc gcacctcgtc gagccgtcga agctgatcgc cggttcgatc
     7141 aaggtctacg gcgagcagct gtannnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7261 nnntcgagcc gtcgaagctg atcgccggtt cgatcaaggt ctacggcgag cagctgtatc
     7321 gcgaactcta cggcgagatc atcccgattc cggccgaccg cgtcctggtc acggaggacc
     7381 ggcaacgcat cgtgctcggc cggcgcacgt tcgagttcat cgacgcgccc ggtcacgcca
     7441 gacatcatca ctgcccgatc gacctcgacc attgcgccgt ctacagcggc gacaacttcg
     7501 ggatctgcta ccgcgagctc gacaccgcgg ccgggccgtt gatgctgccc accacgacgc
     7561 ccgtgcaatt cgagccggac gtgctgcaca agaccatcga tcgcctgatg tcgtatcgcc
     7621 cggtgcgcgt gtaccagacg cacttcgggc cggtggacga cctcgcgcga ctcgccgccg
     7681 acctgcatgc aagcatcgac gagctcgtgc ggatcgcgcg gacgcacgcg cgggcggccg
     7741 accggcgcgc gcgcatcgag gcggacatgt acgcgtactt cgacgcgcgg ctcgacgcac
     7801 acggctgccg gctgtcgcag gccgagcgac accggctcat cgatgacgac gtgcgcctga
     7861 acaccgcggg cctcgaagtt tggctcgacc gctcctgagg ctgaacccat gcccaccttc
     7921 cgtgccttcc ggattcacga aatcgaccgc cgcatcgtcg cgcgtttcga cgacgtctcg
     7981 ctcgacgact tgagtcccgg tgatgtcgta atccgggtga ctcactccga catcaactac
     8041 aaggacgcgc tcgcggcgac cggcaagggc cgcatcctgc gccgatatcc gctcgtcggc
     8101 ggcatcgacc tcgcgggcga ggtcgtgagc tcgacggacg cgcgctatgt cgccggccag
     8161 aaggtgctcg tcaccggttg cggactgtcg gagacgcacg acggaggata tgcggaatac
     8221 gcgcgcgtcc atggcgactg ggtgatcccg gtgccggccg gcatgagcac gctcgactcg
     8281 atgaagatcg gcaccgcggg tttcaccgcc gcgctcgccg tccatcgcat ggaacagaac
     8341 ggacagcagc cgggcaatgg accggtcgtc gtcaccggcg ccaccggcgg cgtcggcagc
     8401 atcgcgatca gcatgctcgc cgggcgtggc tacgaggtcg tggcggtgag cggcaagcca
     8461 gcggccgacg aatacctccg cgcgatcggc gccacgcgga tcctgcacag acaggacctc
     8521 gacctcgggt cgaagccgat ggaaaaagcg ctctgggccg gtgccgtgga caacctcggc
     8581 ggaccggtgc tcacgtggct cacccgcacg accgaccagt ggggcaacat cgcgagcgtc
     8641 gggctcgcgg gcggcgcgga actcgcgacg accgtgatgc cgttcatcct gcgcggcgtc
     8701 aacctgctcg gcatcaattc ggtgctgacg ccgcgcgagc tgaggctaga ggtgtggcgc
     8761 cgcatcgcga cggacctgcg gccagccgcg ctcgaccgca tttgcacgaa ggcagtccga
     8821 ttcgacgaac tgcccgcgca gttcgatgcc tacatcgagg gccgcgtaac gggtcgcagc
     8881 gtggtgacga ttccctagcg ggagtcagcg tcagtccgca gcggcggggg tcgcgcgtcc
     8941 agccagcacg ccgtccgagc cgacttcgac gtccacggcg gggccatcca cgaatgcgcg
     9001 acccgtctgc ttgccgaccg tgaagttggt cgagatgcgc gcgggcgtgc tgtcgatccg
     9061 gcgtgccccc tcggcggtgg gacttgcggg agctgcctgc gcgctgcggg ctgcgcccga
     9121 ggcgaccgcg ttgcccgtct gcgcaagccc ggactgaacc ttttcgccga acgttgcgcc
     9181 ctgtgtctgc ttcggcgtgg aagtaaacgc gagcttatag ctgccaggct tgaggcccgt
     9241 ggcccggaac gatccgtccg ccgcgatcgt cgccgtagtg accgtaccac ccgccgctgg
     9301 cgtgatctgc acgaacttcg cgctcacgat cgtacccccg ggcagcgctg cgccgagtgg
     9361 tgcggccgcg gccggcgcgc cttgcctggc ggtgtcaccc gcatgtgcag gcaggccggc
     9421 tgcgatcgcg aggatcagca cgccgaccgt cgcgggccgg gccatcgaac gcgggttgcg
     9481 ggtgttcatg gtggcgaaca gatacggcga atcgccctgc gatgcaactg cgcgacgagg
     9541 aatgcctcga gccggggccg gtcgttcgag tggatgaatt catcgtccgg aaagcaacct
     9601 tcaaccgtcc tgcaacatcc aacggacatg ctgttctccg cgccatcccg gcgcggccag
     9661 gaggatcgtc atggatatcg cggctgccgt gctgatgctt tcgatcgttc tctacttcat
     9721 ccagcagttc aacgtcgagc catggtaagg gcacgttgaa agcggcgacc ggcacgggat
     9781 gccgtgcccg gtcgctcgca gggactgtct ggcggccagc acctgccgcg atcatgttcg
     9841 acgcctccag attggtattc acccggcgac ttgctacgct cgcgcgcacc tgacttccgg
     9901 gggggaggcg cgccgttgtc tctgttcgcc gagctgaagc gccggaatgt cttccgggtc
     9961 gccgccgcgt acctggccct cggctggatc gtcacgcagg tcacctcgac cgtggcgccg
    10021 atgctgcacc tgccggagtg ggtgggcccg ctggtgctgt ggatcggtgt gatcggcttt
    10081 ccgttcgtgg cgatgttctc gtggatctac gaactgaccc ccgagggcat caggaaggaa
    10141 accgaggtcg accgcacgca gtcgatcacg caccagacgg cgcgcaggct ggactacgtc
    10201 atcattgcgc tgctggtggt ggcaatcgcg ctctttgcgt ggagtcagtt ccggccggtg
    10261 gcgcgcgaga ggctcacggt cgtcgcgaca cctcagtcgg cgacaccggg cgccggtgcg
    10321 gtggaccgct ccgtcgcggt cctgccattc gtggacatga gtgcagcgaa gaaccgggag
    10381 tactttgcgg acggcatctc cgaggaactt ctcaacctgc tcgccaaggt gccgcagctg
    10441 agggtgatcg cgcggacgtc ttccttctcg ttcaagggca aggaagtcga catcgcggag
    10501 atcgcgaaga aactcaacgt cgcaaacgtg ctcgaaggca gcgtgcgcac ttcgggcgac
    10561 aaggtgcgca tcacggccca gctcatccgc gcatcggaca gctcacacct gtggtccgag
    10621 acctacgatc gcaagatgac ggatgtgttc tcggtgcagg acgagattgc cgcggcagtg
    10681 gttgcccagc tcaaggtcac gctgctcggc gccgcgccgt cggcgcgcaa gacggacccg
    10741 cgagcctatg cgctgtacct gcagggccgc gagctcgaat tccagtacag cacggcgtcg
    10801 ctggagcagg cagtcgcgct ctacaagcag gctgtcgcga tcgacccgtc gtatgcgccc
    10861 gcgtgggtgc atctcacctg ggcatacttc agccaggtcg atctcggggt gctgacgccg
    10921 gcccaggggc tggagcggtc gcgcgatgcg ctcggcaagg cgctggcggc cgaccccgcg
    10981 aacgcgttcg cgtacgcaac catggccctg tgggaagggg tcctcgagcg caagtacgag
    11041 gtggccgcca ggcacgtcga gcaggcgctg gcgctcgatc cgacgaattt cgaagcgatc
    11101 gacatcgcgg ctgtcctggc acggcgtctc gggagactcg atctggccat tgcactcggc
    11161 gagtacctgg tcgtgaggga cccggtgaac ccggccaacc acgacgacct tgcgctcgcg
    11221 tacggttaca acggccagct cgacaaggcg atcgccgcgc gccgtaccgt gctgagcctg
    11281 tccccggacg ccggctggca gcacaccgcg ctgggcgatc tgttgctgtt gaagggcgac
    11341 gcgaccgcgg cgctggccga ataccagaag gagccgatcg agtgcttcca cctggtcggc
    11401 ctcaccaagg cgtatcacgc gctaggacgc aaggccgatt ccgacgccgc acgcgccggg
    11461 atgatgcata agtgcgtcga caagaagccc ttcaacgtcg cggcgacatt ggcgtatctc
    11521 ggcgacgtgg acggggcttt ccagatgctc gacaaggccg cgcagataca ggacacggac
    11581 ctgggcgccg tcgccatctt tccggcgttc gccggcatgc acaaggatcc gcgctggctg
    11641 ccgttgctgc gcaggctcgg gctggcgccg gatcagctcg cagcgatcaa actggacgtc
    11701 aaggtgccgc agtagccgca tgcgcggtcc gagtgggacg tggcgcaacc ggcgcggcgg
    11761 ggctgcagga cgcactcgac ggctcgtggc cgcccgcgcg accgtcgcgt ggctaccgct
    11821 cgtcggcacg ctcgcgcacg ccgacggccc ggcgttcgac cggccgggca tcgccttctc
    11881 gtccacgacg atgccggcgc actcggtcac gtgggaacag ggactgctcg acttccagcg
    11941 cgaccaatcg ggtggcgtga cccaggatgc atacgttgtg gatgcccgcc tgcgctatgg
    12001 cctggccgat cgctgggagg tgcagctggc cgcaccgttg tacgacgaga tcgacaccca
    12061 cggatcgggc cagggactca cgaccgcagg cgtcggcgac ctgagtctcg cggtcaagct
    12121 cgcgctgacg ccgcgggacg accggttcac gatgtcgatg ctcggcgtgg tgtcgtttcc
    12181 gacgggcaac gaggacatcg gggccggggc ggagcagtat tcgctcggcg ccacgctggg
    12241 ctggacgttg agcgacagcc agtcactggc cttctacacc aatgtggacc tcctcgcagg
    12301 cgacgcaacg tggacgttcg cgccgagctg gaatttcgac ctcggcgaga ctgtgggagg
    12361 ctacgtcgag gccggctatc agcccgctgc gcatggtgac ccggccaacg cgctggccgg
    12421 cgccgggctc atgtggctgg tgaagccgac ggttcagctc gacgcgtatt tcctcaaggg
    12481 tctgacgtcg gagagcacgg acctggccgc cggctgtggc gtctcgatct tttttccgtg
    12541 aatcccgcgc tcattccagc ggcgcgggcg cacggccggg cgggcgcgtc ttcaccatca
    12601 tcagcgaccg cactttcaac accggcgtgc cgtgctggtt ggcgagcacg ccctcgaacg
    12661 tgatgaggcc gcggtccggc ttcgattccg acgggcgctt ctcgacgcag gtcatcgtga
    12721 acgttaggcg gtcgcccggg cgggcggggt tgggcagctc cagttcatgc ttgatggatg
    12781 cgattccagc cgcgggatcc atgcggctca gcaatcgcgc cgcgaccgca taggtatgga
    12841 cgctcgacgc cgtcagtccc cgcatcggcg agcgctcggc ggcgccgggg tcgacgtgcc
    12901 agggctgcgg atcccattcg cgcgcgaacg cgatgatgtc ctcggccgtc agctcgtgga
    12961 cgcccgcggt ctgtgacgag ccgacttcaa agtcctcgaa atagcgcatg cggcgccatg
    13021 ctattcgatc gagaacggca cgacatggcg ttcgttcggc cgcacggtga ggcgacgtcc
    13081 gagcggcggg aatgcgcgct ggtggcagtc cggccgctcg cagatccggc agctcacgcc
    13141 gaccggcacg gccgccccgt cgagctgcag gccttccgag tacacgacct tctccgcgtg
    13201 cgcgatctcg cacccgatgc cgatcgcgta ctggcggttc ggcctcaggt agtgacccga
    13261 gcgcttgacg atgctgcgcc cgatgcacag gtagcgcacg ccgtccggca tctcggcgac
    13321 ctgcaccagg atgcggcccg gctggccaaa ggcttcgtgc acgttccaca gcgggcaggc
    13381 gcccccgaag cgcgcgaact ggaaacgcgt cgaactgtgg cgcttggtga tgttgcccgc
    13441 ggcgtccacg cgcacgaagt agaacggtac gccacgcgct cccggtcgct gcagcgtgct
    13501 cagccggtgc gacacctgct cgaagctcgc gttgtagcgg t
//
LOCUS       DAOMFD010000045        13359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1119_length_13359_cov_36.357862, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000045 DAOMFD010000000
VERSION     DAOMFD010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13359
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1119_length_13359_cov_36.357862"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1257)
                     /locus_tag="PL152_04305"
     CDS             complement(<1..1257)
                     /locus_tag="PL152_04305"
                     /inference="COORDINATES: protein motif:HMM:NF014443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HQR48533.1"
                     /translation="MLAVVHVLGSMLMLFSITYVLPIATSLIYRDGLLLEFVAAAMIS
                     LGVGAALWVATRRRKRELRSRDGFLLVSLGWALMSAIATVPLLMALPGLSFTDAFFET
                     MSGLSTTGATVLTGIDNLPPSINLWRHALCWYGGMGIIVLVVAILPLLGVGGMQLYKA
                     ETPGPVKNEKLTPRITQTAKALWFIYFLITAACVLSLKVAGMDWFDAVCHAFAALALG
                     GFSTHDASIGYYDSVPIEAVLIVFMLIAAMNFSRHFIAWQQKSLRTYATDTEAKAMLL
                     VIGLATLGVTLFIWHEGVYASFPAAFRHVAFNLVSIATDCGFVSQDYAVWPVFAPIFM
                     LMLSCYCANTGSTGGGIKMFRTLVLFRQAGRELLLLVHPQAVRPVKIAGQVVQNQIVF
                     AVLAFVVLYFGTVVVLTFSLLASGLDF"
     gene            complement(1261..2652)
                     /gene="trkA"
                     /locus_tag="PL152_04310"
     CDS             complement(1261..2652)
                     /gene="trkA"
                     /locus_tag="PL152_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HQR48534.1"
                     /translation="MKIIILGAGQVGRTAAQHLAREESNDVTVVDTDDELLRDLQDRI
                     DIRTVQGNAASPKTLELAGAGDADMIVALTNSDEVNMVACQVAWSLFGTRTKIARIRA
                     QAYTNRPEIFVSTEGDQPPDQLSGFAVDMYVSPEESVTAYIERLIRYPGALQVVDFAD
                     SRVRLVGLRAVKGGLLVGQPIRAIRQHIPQVDARVAAIYRGGHSIDPEGDTVIEHNDE
                     VFFIAATEDIRNVMSEMRKAEDPVKRIVIAGGGNIGLRLAQKLEDGNHQVKIIERSPA
                     RARVISEKLRNTIVLNGDAADEELLVEENIDSADVFCALTNSEEANILSALLAKRLGA
                     HRVMALITRLSYAQLMQAGPIDVAISPQTVTIGSLLAYVRRGDVVMVHSLRGGTAEAM
                     ETIAHGIRGGKVVGRAVEDIKLPEGARIVTLVRGDQVIMAHHDTVVETDDHVILFLAD
                     KRHVEQVERLFQS"
     gene            complement(2668..4860)
                     /gene="uvrD"
                     /locus_tag="PL152_04315"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(2668..4860)
                     /gene="uvrD"
                     /locus_tag="PL152_04315"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006268 - DNA unwinding involved in DNA
                     replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HQR48535.1"
                     /translation="MDVSSILDPLNDAQRQAVAAPLAPTLVLAGAGSGKTRVLTHRVA
                     WLVQVEHVSPHGILAVTFTNKAAGEMRARIEQQLGMPSAPLWIGTFHGIAHRLLRMHW
                     REAGLPQGFSILDAEDQQRLVKRIVRGAGLDEARWVPREVTWFINAQKDEGLRPKHLK
                     DDGDPTRRQMIRLYEAYEDACRRNGVVDFAELLLRSFELWKEVPPLAEHYRARFGHIL
                     VDEFQDTNTIQYAWMKALAGATSIPFVVGDDDQSIYRWRGARVENLQQFRRDFPGVQV
                     FKLEQNYRSTGNILAAANAIIANNGGRIGKKLWTSGGRGEPIRLYRGYNERDEAEFVV
                     NRIRDWIARGGNRRDAAILYRSNAQSRVFEEYLLAARIPYRVYGGLRFFERLEIKDAL
                     AYLRLISNRDDDASFERVVNLPARGIGARTLEVLRAHAKAHARPLWASCAAVEEELGA
                     RATTALRAFLALVGKLDADTRELPLHEQVDHVIQASGLAEHYRREKADKGEARLENLE
                     ELVSAARGFEPESDDMPPLMAFLSHAVLESGEGQAEEWEDCVQMMTLHSAKGLEFPVV
                     FLCGLEDGLFPHQRSIADLQGLEEERRLCYVGTTRAMRQLYLTYAEQRRMHGVDSYGA
                     PSRFIGEIPAELLEEVRPRVQVSRPAYVPSRPARGTPGIQAASRPQRYTDDAPGSLRL
                     GQRVRHEKFGDGVVLSVEGQGSNARIQVNFERQGTKWLMAGYANLQVV"
     gene            complement(5033..>6981)
                     /locus_tag="PL152_04320"
     CDS             complement(5033..>6981)
                     /locus_tag="PL152_04320"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQR48536.1"
                     /translation="VLGQNQYVTWGETTTGFDVTDTYQEQIQALPPLPSGLPQFVTFY
                     QGNAEPVTVIPVTFKYNVVGDHVPDNLATASGGAIPPVVLTIPRRNNGPIVSLDVDPS
                     TGAGTAISVQYTGFSATRELETFRYLNYARNVRDFVAALQFFDAGSQNFIYGDIDGNL
                     GYFSTGEVPLREDMQAFTGAGLPFSGSPPWFIRNGTGGNEWLVDPRPGPLNGTGYQSL
                     PFSELPQALNPRNGFVVNANNDPAGVTLDNNPVNQLRKSGQGVYYLGYTFDFGTRAGR
                     ITEALAARLAQGKVSAKDMKAIQADVTLLDAEFFTPYILQAFDHATTIGAPASLAALA
                     ADPRVAEAIGRLRAWNFTTPTGSANGYDASDVDGMLVAPTSAEVARSIAATIYSVWRG
                     RAINNGVDIALDSLGVPRPGSGDAIKALRHLTEHDGIGLSTVDFFGWTAMVHPDLAQP
                     AQRRDYVMLASLADALDHLAGPSFAAAFGGSTNQNDYIWGRLHRIVFDGLAVGGPFSI
                     PGATPGFPPSFDDLAGLATDGGFGTVDASSHSARADSSNDFMFGSGPNRRYVGVPGTA
                     AGSIDGQTALPGGMSGVLGNKFYANLLGRWLTNDTYPLRQGMGEIMQNLDSQQMFKPA
                     SPGHCDQTPGKSKGKKNDKQPGCH"
     assembly_gap    6982..7081
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7082..8001)
                     /locus_tag="PL152_04325"
     CDS             complement(<7082..8001)
                     /locus_tag="PL152_04325"
                     /inference="COORDINATES: protein motif:HMM:NF013927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0017000 - antibiotic biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HQR48537.1"
                     /translation="MRKAAFVPLGTLLALSLAQAHGNPPGQVKDQSIALPELHGPARI
                     LRDVDGMPHIRAQDEHDALFLQGWVTAQDRLFQIDVLRRQASGTLAELVGPAALGSDV
                     ELRTVGLRRAAERSLAKYSPQMKAALQAYADGVNAWVRQNPVLPAQYGALEITKFQPW
                     SALDSAVIGKALAFQLSFDIDVDPTLQYLEYQAKLGPINPDLPDAMFFGDIFRSAPFD
                     PAASVPDATGTAPFIGSLSKVAAAAAGKMSATPTSRSAPVTVGVGASTSRMLRDLKRR
                     YEAVPFLKNTLQRTEQQIGSNEWGVAGSRTV"
     gene            complement(8175..9332)
                     /locus_tag="PL152_04330"
     CDS             complement(8175..9332)
                     /locus_tag="PL152_04330"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQR48538.1"
                     /translation="MASRSHFAAPLLLAVVVALIVYVSLYPFRFASDGPSLVDAMHVL
                     TWARAGRGEMLNNVLLYMPFGFCIALLVEPRFGRTAGIACALLAGGLLSLYMELLQAS
                     VPTRVASLKDLSLNAAGALAGALLGSGWHLLSARMTPQAVSGARSRAVVITLLALWLL
                     ARLWPLVPDLGLRQLKGAVRPLFSPHVEPGETARYFVGWLVVAQAVFSLAQRQRAVDA
                     FLIAIAVVLAGRMVTTGNTLVASELAAIALLLPVLVLLSRLPDGARAMLVAALLGTSL
                     AWSAVQPVLGHAGPRPVDLPALADFIGRNPPPPAHLAAKGFSYVALAWLLAVAGLVPH
                     VAAGVTFLFALILSMLQLGAAEPSFGWVDVAIAAIAGILVARWMPRPAVRQ"
     gene            complement(9342..10250)
                     /locus_tag="PL152_04335"
     CDS             complement(9342..10250)
                     /locus_tag="PL152_04335"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HQR48539.1"
                     /translation="MPYSPVADAAPGPATWRDYYELGKPRVVALIVFTAIVGMFLAVP
                     GFPPAGPFVFGALGIGLAASSAAAINHVVDRRYDQKMARTMLRPIPTGHLTSAQALTY
                     AAVLGVASMAMLWWLVNPLTAVLTCASLIGYAVLYTVWLKHVTPQNIVIGGAAGAAPP
                     VLGWTAITGHVDPHALLLFLVIFAWTPPHFWALAIARRDDYAKAGIPMLPVAYGVAFT
                     RLHILLYTILLCLVTLLPWLTGMAGLVYLGAAVLLGARFLRHAIALRRAGAPAHLPMA
                     VFRYSIRYLMLLFAALLLDHYVKIGF"
     gene            complement(10253..11254)
                     /locus_tag="PL152_04340"
     CDS             complement(10253..11254)
                     /locus_tag="PL152_04340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HQR48540.1"
                     /translation="MRLTWFRRLCRVAALLALCVVVLGAWTRLGNAGLGCPDWPGCYG
                     HLTVGQAVEHSAEANAAFPDRPLEADKAIKEMVHRYFASTLGLAIIAIAVLAFLNRRD
                     PAQPVRLPALLVLLVVFQGLLGMWTVTLLLKPLIVTAHLVGGLTTMSLLWWLSLDPAP
                     AVQSANDRGLRTLAVTGLAVLALQVMLGGWTSSNYAAIACPDFPTCQASYWPDMDFRD
                     AFVLWRGLGIDYEGGVLDHPARVAIHYTHRLGAVVTALLLGFLAWKAVRTGRSRAVRA
                     AGGAVAVVLVTQWIVGPVMVLKTFPLPLATAHNAVAALLLLAVITLTRYAWTPKRVA"
     gene            complement(11259..11876)
                     /locus_tag="PL152_04345"
     CDS             complement(11259..11876)
                     /locus_tag="PL152_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48541.1"
                     /translation="MNTPDPMPVRRGRLQMLALAALFFVPLALAFWLYYGPHAWRPSG
                     SASQGDLVDPPRPLPEFDLAVAGGSPADAHWLRGKWTMLYIGDGACDERCRKALYLTR
                     QSRTALNRDADRVRRVFVIYGSDYDYDFVEREHPDLVVVMLGGQDGRDLLARLPVYGD
                     GPPGTAGRIYLVDPLGNLMMSYPANAPDKALLKDLQRLLRLSHIG"
     gene            complement(11873..12625)
                     /locus_tag="PL152_04350"
     CDS             complement(11873..12625)
                     /locus_tag="PL152_04350"
                     /inference="COORDINATES: protein motif:HMM:NF014193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HQR48542.1"
                     /translation="MRVDFGSRSFAPRRWAVIVTAVMLAACIALGTWQLGRSREKQAL
                     IDAFERGDTGTVPLTVASVDELPRYQRVSVTGRYQPDRQILLDAMPSSTGQAGYRVLT
                     PLQRADGGKLLLVDRGWVPMGLDRGHLPDVSVTGVPRTVHGRLDQLPVPGLRVGAARA
                     PGASGWPLVLLFPVVADLETALGEPVEPRIVLLDARDPDGYEREWRPSLAVPPERHLG
                     YAIQWFALALVALVAFVATGLQPRTSKAQDHP"
     gene            12665..12871
                     /locus_tag="PL152_04355"
     CDS             12665..12871
                     /locus_tag="PL152_04355"
                     /inference="COORDINATES: protein motif:HMM:NF022587.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HQR48543.1"
                     /translation="MIKFLIVATLAAIVASLGTGLFHLVKDEGQSKRMVNALTVRIAL
                     SVLLFVLLFIAWRGGLIAPHGIGR"
     gene            complement(12971..>13359)
                     /locus_tag="PL152_04360"
     CDS             complement(12971..>13359)
                     /locus_tag="PL152_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HQR48544.1"
                     /translation="ILLTSGVTITIAHHALKAGHRGILKLFLGLTFLLGFLFVYMQAH
                     EYMEAYEHFNLTLGSGVYGSTFFMLTGFHGLHVTIGAIMLTVIWLRVMKGHFTPTHHF
                     AFEGVAWYWHFVDVVWLGLFIFVYWL"
BASE COUNT         2115 a         4452 c         4726 g         1966 t
ORIGIN      
        1 gaagtcgagc ccgctcgcga gcagcgagaa ggtgagcacc accaccgtgc cgaagtacag
       61 cacgacgaat gcgagcaccg cgaacacgat ctggttctgc acgacctgtc ccgcgatctt
      121 gaccgggcgc acggcctgcg ggtgcaccag cagcaggagt tcacggccgg cctgccggaa
      181 cagcaccagc gtgcggaaca tcttgatgcc gccgccggtc gacccggtat tggcgcagta
      241 gcacgacagc atcagcatga agatcggcgc gaacaccggc cagacggcgt agtcctggct
      301 cacgaagccg caatcggtcg cgatcgagac gaggttgaac gccacgtgcc ggaacgcagc
      361 cggaaacgac gcgtacacgc cctcgtgcca gatgaacagc gtcacgccga gcgtggcgag
      421 gccgatcacc agcagcatcg ccttggcctc ggtgtcggtc gcgtaggtcc tgaggctctt
      481 ctgctgccag gcgatgaagt gccgcgagaa gttcatcgcg gcgatcagca tgaacacgat
      541 cagcaccgcc tcgatgggca cggagtcgta gtagccgatg ctcgcgtcgt gggtcgagaa
      601 tccgcccagg gcgagcgcgg cgaaggcgtg gcacacggcg tcgaaccagt ccatgccggc
      661 aaccttcagg gaaagaacgc acgccgcggt gatcaggaag tagatgaacc agagcgcctt
      721 cgccgtctgc gtgatgcgcg gggtcagctt ctcgttcttg accggtcccg gcgtctcggc
      781 cttgtagagc tgcatgccgc cgacgccgag cagcggcagg atcgcgacca ccagcacgat
      841 gatgcccatg ccgccgtacc agcacagcgc gtggcgccag aggttgatcg acggcggcag
      901 gttgtcgatg cccgtcagca cggtcgcgcc ggtggtgctc aggcccgaca tcgtctcgaa
      961 gaacgcgtcc gtgaacgaca ggcccggcag cgccatgagc agcggcacgg tcgcgatcgc
     1021 cgacatgagc gcccagccca ggctcaccag caggaatccg tcgcgcgagc gcaattcgcg
     1081 cttccggcgc cgcgtcgcca cccacagcgc cgcgcccacg cccaggctga tcatggcggc
     1141 cgccacgaac tcgagcagca gcccgtcgcg gtagatgagc gaggtcgcga tcggcaggac
     1201 gtaggtgatg ctgaacagca tcagcatcga gcccaggacg tggacgaccg caagcatcgg
     1261 tcaggactgg aacagccgct cgacctgctc gacgtgccgc ttgtcggcga ggaacaggat
     1321 cacgtggtcg tcggtctcga ccaccgtgtc gtggtgcgcc atgatcacct ggtcgccacg
     1381 gaccagcgtc acgatgcgcg cgccctccgg cagcttgatg tcctccactg cgcgcccgac
     1441 gaccttcccg ccgcggatgc cgtgcgcgat ggtctccatc gcctcggcgg tgccgccgcg
     1501 cagcgagtgg accatgacga cgtcgccgcg ccggacgtag gcgagcagcg acccgatggt
     1561 caccgtctgc ggcgagatcg cgacgtcgat cggccccgcc tgcatgagtt gcgcgtacga
     1621 cagccgggtg atcagcgcca tgacgcggtg ggcgccgagg cgcttggcga gcagcgcgga
     1681 gaggatgttc gcttcctcgg aattcgtgag cgcgcagaag acgtcggcgc tgtcgatgtt
     1741 ctcctcgacc agcagttcct cgtcggcggc gtcgccgttc agcacgatcg tgttgcgcag
     1801 cttctccgag atcacgcgcg cgcgggccgg cgaccgctcg atgatcttca cctggtggtt
     1861 gccgtcctcg agcttctgcg cgagccgcag gccgatgttg ccgccaccgg cgatcacgat
     1921 gcgcttgacc gggtcctcgg ccttgcgcat ctcgctcatc acgttgcgga tgtcctcggt
     1981 ggccgcgatg aagaacacct cgtcgttgtg ctcgatgacg gtgtcgccct cggggtcgat
     2041 gctgtggccg ccgcggtaga tcgccgcgac tcgcgcgtcc acctgcggga tgtgctggcg
     2101 gatcgcgcgg atcggctggc ccacgagcag cccgcccttg acggcccgga gcccgacgag
     2161 tcgcacgcgg gagtcggcga agtcgacgac ctgcagggcg ccggggtagc ggatcaggcg
     2221 ctcgatgtag gcggtgacgg actcctcggg gctcacgtac atgtccaccg cgaagcccga
     2281 gagctggtcg ggcggctggt cgccctccgt ggacacgaag atctccgggc ggttggtgta
     2341 ggcctgcgcg cggatcctcg cgatcttggt gcgggtgccg aacaggctcc acgcgacctg
     2401 gcaggcgacc atgttgacct cgtcgctgtt ggtgagcgcg acgatcatgt cggcgtcgcc
     2461 ggccccggcg agttccagcg tcttcgggct cgcggcgttg ccctgcacgg tgcggatgtc
     2521 gatccggtcc tgcaggtcgc gcagcagctc gtcgtccgtg tccaccacgg tgacgtcgtt
     2581 cgattcctcc cgcgcgaggt gctgggccgc ggtgcgtccg acctggccgg ccccgagaat
     2641 tatgattttc accgcggcat tgtagcgcta gacgacctgc aggttggcgt agcccgccat
     2701 gagccacttg gtgccctggc gctcgaagtt cacctggatc cgtgcgttcg agccctggcc
     2761 ttccacgctc aggaccacgc cgtcgccgaa cttctcgtga cgcacgcgct gcccgaggcg
     2821 caggctcccc ggcgcatcgt ccgtgtagcg ctgcgggcgc gacgcggcct gtatcccggg
     2881 cgttccccgg gccgggcggg acgggacgta ggccgggcgc gaaacctgaa ccctcggccg
     2941 cacttcctcg aggagttcgg cggggatctc gccgatgaag cgcgacggcg ccccgtagct
     3001 gtcgacgccg tgcatgcgac gctgctccgc gtaggtgagg tagagctgcc gcatcgcccg
     3061 ggtcgtgccc acgtaacaga ggcggcgctc ctcctcgagt ccctggaggt cggcgatgga
     3121 gcgctggtgc gggaacagcc cgtcctccag gccgcagagg aacacgaccg ggaactcgag
     3181 tcccttggcg gagtgcagcg tcatcatctg cacgcagtct tcccactcct ccgcctggcc
     3241 ctcgccggac tcgagcacgg cgtgcgacag gaacgccatg agcggcggca tgtcgtcgct
     3301 ctccggctcg aagccgcgcg cggcgctcac cagctcctcc aggttctcga ggcgcgcctc
     3361 gcccttgtcg gccttctccc ggcggtagtg ctccgcgagc ccgctcgcct ggatgacgtg
     3421 atcgacctgc tcgtgcagcg gaagttcgcg ggtgtcggca tcgagcttcc cgacgagcgc
     3481 aaggaaggcc cgcagggccg tggtcgcgcg tgcgccgagc tcctcttcga cggcggcgca
     3541 ggacgcccac aacggccggg catgagcctt tgcgtgggcg cgcagcacct ccagcgtacg
     3601 cgcgccgatg ccacgggccg gcaggttcac gacgcgctcg aacgacgcgt cgtcgtcacg
     3661 gttcgagatc agccgcaggt atgcaagcgc gtccttgatc tccaggcgct cgaagaaccg
     3721 cagcccgccg tagacgcggt aggggatgcg cgccgccagc aggtactcct cgaagacccg
     3781 tgactgcgcg ttggagcggt agaggatggc cgcatcgcgg cggttgcccc cgcgagcgat
     3841 ccagtcgcgg atgcggttga cgacgaactc ggcctcgtcg cgctcgttgt atccgcgata
     3901 gaggcggatc ggctcgccgc gcccgccgct ggtccagagc ttcttgccga tgcggccgcc
     3961 gttgttggcg atgatcgcgt tcgccgccgc aaggatgttg ccggtcgacc ggtagttctg
     4021 ctcgagcttg aacacctgca cgccggggaa gtcgcggcgg aactgctgca ggttctcgac
     4081 gcgcgcgccg cgccagcggt agatcgactg gtcgtcgtcg ccgacgacga acgggatgct
     4141 ggtcgcgccg gccagcgcct tcatccaggc gtactggatg gtgttggtgt cctggaactc
     4201 gtcgacgagg atgtggccga agcgcgcgcg gtagtgctcg gcgagcggcg ggacttcctt
     4261 ccacagttcg aagctgcgca gcagcagctc cgcgaagtcc accaccccgt tgcgccggca
     4321 cgcgtcctcg taggcctcgt agagccggat catctggcgg cgcgtcgggt cgccgtcgtc
     4381 cttcaggtgc ttcgggcgca gcccttcgtc cttctgggcg ttgatgaacc aggtcacctc
     4441 gcgcggcacc cagcgcgcct cgtcgagccc cgcgccgcgc acgatgcgct tgacgagccg
     4501 ctgctggtcc tcggcgtcga ggatcgagaa tccctgcggc aggccggcct cgcgccagtg
     4561 catcctgagc aggcggtgcg cgatgccgtg gaaggtgccg atccagagcg gggccgaggg
     4621 catgccgagc tgctgctcga tgcgcgcgcg catctcgcct gcggccttgt tggtgaaggt
     4681 gactgcgagg atgccgtgcg gggagacgtg ctccacctgc accagccacg ccacgcggtg
     4741 ggtgagcacg cgcgtcttgc cgctgccggc gccggcgagc accagcgtcg gtgcgagcgg
     4801 cgccgcgacg gcctgccgct gcgcgtcgtt cagcgggtcg aggatgctgg agacgtccat
     4861 ggaagggcgg cgattctatc ggggacattc tcctatttcg aatcgaaata ggagaatgtc
     4921 ccctggcccg gccatcggcc ccgccgcggg tccgtgaact ggggaacgcc tgccgtgcct
     4981 ggccctcccc gcgcgcaggc ggggagggcc agcaacggtg aatcggctgg cgtcagtggc
     5041 agcccggctg cttgtcgttc ttcttgccct tgctcttgcc cggcgtctgg tcgcagtggc
     5101 ccggcgatgc gggcttgaac atctgctggc tgtcgaggtt ctgcatgatc tcgcccatgc
     5161 cctgccgcag cggataagtg tcgttggtga gccagcggcc gagcaggttc gcgtagaact
     5221 tgttgccgag cacgccgctc atgccgccgg gcaacgcggt ctgcccgtcg atcgagccag
     5281 cggccgttcc cgggaccccg acgtagcggc ggttgggtcc gctgccgaac atgaagtcat
     5341 tggacgagtc cgcccgggcg ctgtgcgacg aggcgtccac ggtcccgaag ccgccgtcgg
     5401 tggcgagccc ggcgaggtcg tcgaacgacg gcgggaaccc cggggtcgcc cccggaatgc
     5461 tgaacgggcc gccgacggcg aggccgtcga agacgatccg gtgcagccgg ccccagatgt
     5521 agtcgttctg gttggtcgat ccgccgaatg ccgccgcgaa cgacggtccc gcgagatggt
     5581 cgagcgcatc ggccaggctc gccagcatca cgtagtcgcg acgttgcgcc ggctgcgcga
     5641 ggtcagggtg caccatcgcc gtccagccga agaagtcgac ggtcgacagc ccgatgccgt
     5701 cgtgctccgt caggtggcgc agcgccttga tggcgtcgcc cgagccgggc ctcggcacgc
     5761 cgagactgtc gagcgcaatg tccaccccgt tgttgatcgc gcggccgcgc cacaccgagt
     5821 agatcgtcgc ggcgatgctg cgtgcgacct cggcgctggt gggcgccacg agcatgccgt
     5881 cgacgtcgga cgcgtcgtag ccgttcgccg agccggtcgg agtcgtgaaa ttccacgccc
     5941 ggagccggcc gatcgcttct gcgacgcgcg ggtcggcggc caatgcggcg agcgacgcag
     6001 gtgcgccgat ggtggtcgca tggtcgaacg cctgcagtat gtacggcgtg aagaactcgg
     6061 cgtccagcag ggtgacgtcc gcctggatgg ccttcatgtc cttcgcgctg actttccctt
     6121 gcgcgagcct ggcggccagt gcctcggtga tgcggccggc gcgcgtgccg aaatcgaacg
     6181 tgtagccgag gtagtagaca ccctggccgg acttgcgcaa ctggttgacc gggttgttgt
     6241 ccagcgtcac gccggccggg tcgttgttgg cattgacgac gaagccgttg cggggattga
     6301 gcgcctgcgg caattccgag aacggcagcg actggtaacc ggtgccgttc agcgggccgg
     6361 gcctgggatc caccagccac tcgttgccgc ccgtgccgtt gcggatgaac cacggcggag
     6421 acccgctgaa tggcagtccc gcgcccgtga acgcctgcat gtcctcgcgc agcggcacct
     6481 cgcccgtgga gaagtacccg aggttgccgt cgatgtcgcc gtagatgaag ttctgcgagc
     6541 cggcatcgaa gaactggagc gccgccacga agtccctgac gttgcgggcg tagttgaggt
     6601 aacggaacgt ctccagctcg cgcgtggcgc tgaagccggt gtactgcacg ctgatggccg
     6661 tcccggcgcc cgtcgacggg tcgacatcga ggctcacgat cgggccgttg ttacggcgcg
     6721 ggatggtcag cacgaccggc gggatggcac caccgctcgc ggtcgcgaga ttgtcgggga
     6781 catggtcgcc cacgacgttg tacttgaacg tgaccgggat gaccgtgacg ggctcggcgt
     6841 tgccctggta gaacgtgacg aactgcggca gaccgctcgg cagcggcggc agcgcctgga
     6901 tctgctcctg gtaggtgtcg gtgacgtcga agccggtcgt cgtctctccc cacgtgacgt
     6961 actggttttg gcccagcacg annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 naccgtgcgc gatccggcga cgccccactc gttgctgccg atctgctgct ccgtgcgttg
     7141 caaggtgttt ttcaggaatg gcacggcctc gtagcggcgc ttcaggtcgc gcagcatcct
     7201 gctcgtggat gcgcccacac cgacggtgac cggcgccgac cgcgaagtcg gggtggcact
     7261 catctttccg gcggccgcgg ccgcaacctt gctcaggctc ccgatgaacg gcgcggtgcc
     7321 ggtcgcgtca ggcacgctgg cggccgggtc gaacggcgcc gaccggaaga tgtcaccgaa
     7381 gaacatcgcg tccggcaggt ccgggttgat cggcccgagc ttcgcctggt actcgaggta
     7441 ctgcagcgtc ggatcgacgt cgatgtcgaa cgacagctgg aacgccagcg ctttgccgat
     7501 cacggcgctg tcgagcgccg accacggctg gaacttcgtg atctcgagcg cgccgtattg
     7561 cgcaggcagc acgggattct gccgaaccca ggcgttcacg ccgtcggcgt aggcctgcag
     7621 cgccgccttc atctgcggcg agtacttcgc gagcgagcgc tcggcggcgc gccgcaggcc
     7681 gaccgtgcgc agctcgacgt cgctgcccag agcggcggga ccgaccaatt ccgcgagagt
     7741 gccgctcgcc tgccgacgca gcacgtcgat ctggaacagg cggtcctgcg cggtgaccca
     7801 gccctgcagg aacaaggcat cgtgctcgtc ctgcgcgcgg atgtgcggca tgccgtccac
     7861 gtcccgcagg atgcgcgcgg gaccatgcag ttccgggagc gcgatcgact ggtccttgac
     7921 ctggcccggc ggattgccgt gggcctgtgc gagggaaagg gcgagtagcg tgccgagcgg
     7981 cacgaaggcg gctttgcgca tgtgtgtatc ccctggtgcg ttatctggag ccgggcgtcg
     8041 agcaacggcc cttcgccgtt tatacgccga gggtgtgcgc gaggaaacgc ccaattcgcc
     8101 cggaatttgt cataacgccc gccggatgag agtggttcgc gcccagccga tccccgtacg
     8161 cgacgggggc ttcgtcactg tcgcacggca ggccgcggca tccaccgcgc gaccaggatg
     8221 ccggcgatcg cggcgatcgc gacgtccacc cagccgaacg aaggctccgc cgcgccgagc
     8281 tgcagcatgc tcaggatcaa tgcgaacagg aaggtgacgc cggcggccac gtgcggcacg
     8341 agcccggcca cggcgagcag ccacgcgagc gcgacgtaac tgaagccttt cgccgccagg
     8401 tgggccggtg gcggaggatt gcggccgatg aagtcggcga gcgccggcag gtccaccggc
     8461 ctgggcccgg catgacccag gaccggctgc accgcggacc aggcgagcga cgtgccgagg
     8521 agcgccgcga ccagcatcgc acgcgcgccg tccggcagcc ggctcagcaa caccagcacc
     8581 ggcagcagca gcgcgatggc ggcgagttcc gacgccacga gcgtgttgcc ggtcgtcacc
     8641 atgcggccgg ccagcacgac ggcgatcgcg atcaggaacg cgtcgacggc gcgttgccgc
     8701 tgcgccaggc tgaacaccgc ctgtgcgacc accagccagc cgacgaagta acgagcggtc
     8761 tctccgggct cgacgtgcgg tgagaacagc ggacggacgg cgcccttgag ctggcgcagc
     8821 ccgaggtccg gcaccagtgg ccacaggcgc gcgagcagcc agagcgcgag caacgtgatg
     8881 accacggctc gcgagcgcgc gccggacacg gcctggggag tcatgcgcgc gctgagcagg
     8941 tgccagcccg agccgagcaa ggcgccggcg agcgcgccgg cggcattgag cgacaggtcc
     9001 ttgaggctgg ccacgcgcgt cggcacggac gcctgcagca gttccatgta gagcgacagc
     9061 aggccgccgg ccagcaatgc acaggcgatg cccgccgtgc ggccgaagcg gggctcgacg
     9121 agcagcgcga tgcagaatcc gaacggcatg tagagcagca cgttgttcag catttcgccg
     9181 cggccggcgc gggcccacgt cagcacgtgc atcgcgtcga cgagcgacgg gccgtccgac
     9241 gcgaagcgga acggatacag cgatacgtag acgatcagcg cgacgacgac cgcgagcagc
     9301 agcggggcgg cgaaatgcga acggctggcc atctgcgcag gctagaaacc gatcttcacg
     9361 tagtgatcca gtagcagtgc cgcgaacagc agcatcaggt acctgatcga gtagcggaac
     9421 acggccatcg gcaggtgcgc gggagcgccc gcccgccgca gcgcgatcgc gtggcgcagg
     9481 aaccgcgcgc cgagcagcac cgccgcgccg aggtagacga gtcccgccat gccggtcagc
     9541 cacggcagca gcgtcaccag gcacagcagg atggtgtaga ggaggatgtg caggcgggtg
     9601 aaggccacgc cgtaggcgac cggcagcatc gggatgccgg ccttggcgta gtcgtcgcgg
     9661 cgcgcgatcg cgagcgccca gaagtgcggc ggcgtccacg cgaagatgac gaggaacagc
     9721 agcagcgcgt gcggatcgac gtgaccggtg atcgcggtcc agccgagcac gggcggcgcc
     9781 gcgccggccg cacctccgat cacgatgttc tgcggcgtga cgtgcttgag ccacacggtg
     9841 tagagcaccg cgtagccgat gagcgaggcg caggtgagca ccgcggtcag ggggttgacg
     9901 agccaccaca gcatcgccat cgacgccacg ccgagcacgg cggcgtaggt gagtgcctgc
     9961 gcggaggtca ggtggccggt cgggatgggt cgcagcatcg tgcgcgccat cttctggtcg
    10021 tagcgccggt ccacgacgtg gttgatcgcg gccgcgctcg atgccgccag cccgatgccg
    10081 agcgcgccga acacgaacgg gcccgcgggc gggaagcccg gcacggcgag gaacatgccc
    10141 acgatcgccg tgaagacgat caacgccacg acccgcggct tgccgagctc gtagtagtcg
    10201 cgccaggtgg ccggacccgg tgcggcgtcg gcgacgggcg agtacggcac ggtcaggcca
    10261 cgcgcttcgg tgtccaggcg tacctggtga gcgtgatcac ggccagcagc agcagcgctg
    10321 cgacggcgtt gtgcgccgtc gcgagtggca gcgggaacgt cttcagcacc atcaccgggc
    10381 cgacgatcca ctgcgtcacg agcacgaccg cgaccgcccc gcccgcggcc cgtaccgcac
    10441 gcgaccggcc ggtccggacg gccttccacg ccaggaatcc caggagcagg gccgtgacga
    10501 cggctccgag gcgatgcgtg tagtggatgg cgacgcgcgc cggatgatcg aggacgccgc
    10561 cctcgtagtc gatgccgagc ccgcgccaca gcacgaacgc gtcgcggaaa tccatgtccg
    10621 gccagtagct ggcctggcag gtcggaaagt cggggcacgc gatcgccgcg tagttcgaac
    10681 tcgtccagcc gccgagcatg acctgcagcg cgagcactgc gaggccggtg accgcgagag
    10741 tgcggagccc gcggtcgttc gccgactgca cggctggggc tgggtcgagg ctgagccacc
    10801 acagcaggga catcgtggtg agcccaccga cgaggtgggc cgtcacgatc aggggtttca
    10861 gcagcagtgt cacggtccac atgccgagca gcccctggaa cacgacgagc agcaccagca
    10921 gcgcaggcag ccgcaccggt tgtgcgggat ccctgcggtt caggaaggcg aggaccgcaa
    10981 tcgcgatgat cgcgagcccg agcgtcgagg cgaaatagcg atgcaccatc tccttgatgg
    11041 ccttgtccgc ctcgagtggc cggtccggga aggcggcatt ggcctcggcg ctgtgctcga
    11101 cggcctgtcc gaccgtcagg tgcccgtagc agccgggcca gtcggggcag ccgagcccgg
    11161 cgttgccgag ccgggtccac gcgccgagca ccacgacgca cagggcaagc agcgccgcca
    11221 cgcggcacag tcgacggaac caggtcagcc tcatggagtc acccgatgtg tgagagccgc
    11281 agcagcctct gcaggtcctt gagcagtgcc ttgtccggag cgttcgcggg atagctcatc
    11341 atgaggttgc cgagcgggtc gacgaggtag atgcgcccgg cggtgcccgg cgggccgtcg
    11401 ccgtacaccg gcagccgcgc gagcaggtcc ctgccgtcct gaccgccgag catgaccacc
    11461 acgaggtccg gatgctcgcg ctcgacgaag tcgtagtcgt agtcgctgcc gtagatcacg
    11521 aagacgcggc gcacgcggtc ggcatcccgg ttgagcgccg tgcgcgactg gcgggtgaga
    11581 tacagggctt tgcggcagcg ctcgtcgcat gcgccgtcgc cgatgtagag catcgtccac
    11641 ttgccacgca gccagtgcgc atcggcgggc gacccgccgg cgacggccag gtcgaactcc
    11701 ggcagcgggc gcggcgggtc gacgaggtcg ccctggctgg cgctgccgct gggtcgccag
    11761 gcatgcgggc cgtagtagag ccagaacgcg agcgcgagcg gaacgaagaa cagcgcagcg
    11821 agcgccagca tctggagccg gccgcgtcgc accggcatcg ggtccggggt gttcatgggt
    11881 ggtcctgggc ctttgatgtg cggggctgga ggcccgtcgc gacgaacgcg acgagcgcca
    11941 ccagcgcgag cgcgaaccat tgtatcgcgt agccgaggtg acgttccggc gggacggcga
    12001 gcgacggccg ccactcgcgc tcgtagccgt cggggtccct ggcgtcgagc agcacgatgc
    12061 gcggttccac gggctcgccg agcgcggtct cgaggtcggc gacgaccggg aacagcagta
    12121 ccaggggcca gcccgacgcc ccgggcgcgc gcgccgcacc gacgcgcagc cccggcacgg
    12181 gcagctggtc gagtcggccg tgcacggtgc gcggcacgcc ggtcaccgat acgtccggga
    12241 ggtggccgcg atccaggccc atcggcaccc agccgcggtc cacgagcagc agtttcccgc
    12301 cgtcggcgcg ctgcaatggc gtcaaaacgc gatagccggc ctgcccggtg ctggagggca
    12361 tggcgtcgag caggatctgc cggtccggct ggtatcggcc ggtgacggac acgcgctggt
    12421 agcgcgggag ttcgtcgacc gaggcgacgg tcagcggcac cgtacccgtg tcgccgcgct
    12481 cgaaggcgtc gatgagcgcc tgcttctcgc gtgacctgcc gagctgccag gtgcccagcg
    12541 cgatgcaggc cgcgagcatc acggcggtca cgatgaccgc ccagcgccgg ggggcgaacg
    12601 agcggctgcc gaagtcgact ctcatctata attgcgccgt tccgccgcgc cgctgccgcc
    12661 cgacatgatc aagttcctca tcgtcgccac gctggcggcc atcgtcgcaa gcctgggcac
    12721 cgggctgttc cacctggtca aggacgaagg ccagagcaag cgcatggtca atgcgctgac
    12781 ggtgcgtatc gcgctgtcgg tgctgctgtt cgtgctgctg ttcatcgcct ggaggggcgg
    12841 gctcatcgct ccgcacggca tcggccgctg agcggcgggc cgctccacgc ctcgagacgg
    12901 aacggccgcc cgagggcggc cgtcctgcct ggctagccgg gcgccgtcgc agggcgaccg
    12961 ttcgctcgcg ctacagccag tagacgaaga tgaacagccc gagccacacc acgtcgacga
    13021 agtgccagta ccacgccacg ccctcgaagg cgaagtggtg cgtcggcgtg aagtgcccct
    13081 tcatgacacg cagccagatc acggtcagca tgatcgcgcc gatggtcacg tgcagtccgt
    13141 ggaagcccgt cagcatgaag aacgtcgagc cgtacactcc gctgccgagc gtcaggttga
    13201 agtgctcgta ggcctccatg tactcgtgcg cctgcatgta gacgaacagg aagccgagca
    13261 ggaaggtgag cccgaggaac agcttcagga tgccgcgatg ccccgccttg agcgcgtggt
    13321 gcgcgatcgt gatggtcacg cccgaggtga gcaggatcg
//
LOCUS       DAOMFD010000046        13123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1156_length_13123_cov_36.583333, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000046 DAOMFD010000000
VERSION     DAOMFD010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13123
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1156_length_13123_cov_36.583333"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..454)
                     /locus_tag="PL152_04365"
     CDS             complement(<1..454)
                     /locus_tag="PL152_04365"
                     /inference="COORDINATES: protein motif:HMM:NF013673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HQR48545.1"
                     /translation="MRPDIPPALFAPRQPLVPSGLARDPLALARGALLAPAGLDESRV
                     AGVLGEVMGHAVDYADAYFQLIREESWALEDGIVKDGAHSIDQGVGVRALAGEKTGFA
                     YSDEVVLPAMLEAAHAARAIARSGSGGSVQAWHARGGRGLYLPMDPVES"
     gene            complement(460..1275)
                     /locus_tag="PL152_04370"
     CDS             complement(460..1275)
                     /locus_tag="PL152_04370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HQR48546.1"
                     /translation="MTVVAAIQMTSTPDVRRNLADAERLLREASSAGAAVAALPENFA
                     FMGMAEADKLSIAETEGHGDIQQFLSGLARELRLWVIAGTIPLRVAGQPRVAAASLVY
                     DARGTQVARYDKIHLFDVEVPGRDERYQESSSVRPGDACVCVDTPAGRVGLAVCYDIR
                     FPELFRRLLEMGAEWFCLPSAFTVPTGRAHWETLLRARAIENLCHMVAPAQSGFHENG
                     RETHGDSMIVDCWGRVLARLPRGTGVVAADLDLVRQREVRQNFPSVAHRRLQA"
     gene            complement(1272..5216)
                     /locus_tag="PL152_04375"
     CDS             complement(1272..5216)
                     /locus_tag="PL152_04375"
                     /inference="COORDINATES: protein motif:HMM:TIGR02099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdP family protein"
                     /protein_id="HQR48547.1"
                     /translation="MTAAIVAGLLITAALAIGALRLAIARLPENAARLQAWVERQTDL
                     RVEYDALDARLRWFGPEVVLRGVRILQKDGSQALFRSREGSVGLDLWSLFRTGELVAG
                     RVRFTGPTLTVVRLEDGRIRLLGQNERPLDRPPFDLDRLPAGRVEVTDATVTYRDLVT
                     RAAPVTLEQLHFELRRQHDSTALTGAARLPDSLGHAIEFDGDLKGSLEHFDALDAGLE
                     LRMDRLVLAGLSPFLPATIARPLAGAGAVDAMIHFAGGQLTHARLDLALQDVSLALPP
                     RAAPPVSTLTLSAPERPAGASPMTLPHVTSTVADRPADTGPTQVQYPNLRGSVRLRHD
                     GETWTIRAQKLLLYPSGRPGVVPTSFSVRWRGDLATTFDAALSATDLRIHDVWPLALA
                     FAPHALDRWAGLNPTGEIHTFRIEASRDRAGASPRFAMSADVEGVGFSASGTAPGIAG
                     FTGVMSGTDQGGRLSLRSQAFTFDLPRMFREPIAGVAVSADVDWKRDGDAWVIGSGNV
                     AVVHPGARAHGSFEYRYERPGISPFLDMEIQVDHAEVALAGRVLPYGSFGSGAVSWLA
                     PAFLAGQVDAGHVSYRGRVHDFPFKNGEGDFTATASLSGVGVDYFAGFAPLEKGQGIV
                     EFHNAGFVARMHGGEVGGLRLDHAEVGIADMHEAVVEVDATASGDLARALPFVQRSPV
                     GAVLGGQFMGLTGQGPADYSVKLHLPTLPDEPHDLVIRTNLRSATVGIPALRAPAERV
                     TGTFEVHNLEMRAHTLRGSMLDGPFELDVEPGPLTRDVDASVLMHGRGHSVGAGLPAF
                     IGLPPGIRMSGAADWKFEGRMERHRGTEQWTSRYDIASDLVGLGVSAPEPFPKAPSDA
                     RPTHVGLTFAPSGLSEIAVESGPARVRLQLTQGADGRVRLDRGIARFDGRPLVMPERP
                     GLRFAGDWPSFDLGEWLALDTGGKGGESISQWLGPTEVHVDKARIFGFEFEQLDATLK
                     PQPAALQVQVSGPLAEGDVTIPDDLHGGSPIRFAMDRLHLRPAAESGSQASATVDPRK
                     VPALDMDIGDFAWGERRFGHLVASVTKEAMGLSLSGATATTADTTITAQGEWLAEGSG
                     SRTRLGLELRSSNLGATSKALGIPGALEAERATARASVSWPDGPTGSVLAHLNGTLGI
                     SLDRGQLRNVKPGAGRMLGLTSLAELPRRLSLDFHDVTDAGLAFDSVRGDFEIRDGNA
                     YTQNLLLKGPAVDIGIAGRTGLATEDYDQMVVVSGNPAGPATVAGALAGGPIGAAGGF
                     LISQLFKGQLQGLARVYYHVTGPWADPVVERVSSQVGEAPTTAPPPEQDVKQ"
     gene            complement(5258..6727)
                     /gene="rng"
                     /locus_tag="PL152_04380"
     CDS             complement(5258..6727)
                     /gene="rng"
                     /locus_tag="PL152_04380"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HQR48548.1"
                     /translation="MSTEMVVNVSPRETRAALLENGVLQELFVERASKRGLTGNLYKG
                     RVSRVLPGMQAAFMDIGLERTAFLHVSDIVQPADAEGSGDAPRTDNIRDLVGEGSDIL
                     VQVLKDPLGTKGARLTTFITIPSRYLVYMPFGRGVGVSARIENEEERQRLRDAVNAAA
                     APGDTGGYIVRTAAEGAAAEALRADRLFLRKLWEVLTDKARAAGQGTLVHEDLPLALR
                     LMRDLVGEGVDRVLVDNERTCDRMREFAATFMPGLETRVERYTGVRPIFDLHGVEEEI
                     QRSLDRKVALKSGGYLIIDQTEALTTIDVNTGAYVGHRNLEETIYRTNLEAAVAIARQ
                     LRLRNLGGIIIIDFIDMEEEEHRRQVLQALEKALAKDHAKTHFSAVSPLGLVEMTRKR
                     TRESLVHQLCMPCPACEGRSFVKTPETVCYEIFREILRQAAQFDLQQLLVLAHQDVIE
                     RLLDEESAALGELEVQLGKPIRLQTESLYMLDQYDVVLM"
     gene            complement(6724..7320)
                     /locus_tag="PL152_04385"
     CDS             complement(6724..7320)
                     /locus_tag="PL152_04385"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HQR48549.1"
                     /translation="MQRPVICLASASPRRSELLRQIGVAHEARPAEVDESLLAGEAPA
                     AYVRRLAEAKARALWSRGTDARRLPVLAADTTVELDGEIFGKPADRDEALAMLKRLAG
                     RTHEVHTAVAVLHASGSASRLSTSQVTMRAITPAELDWYWSTGEPADKAGAYAVQGRA
                     AIFITHISGSYSGVMGLPLCETWELLTAVPGLIAGGAQ"
     gene            complement(7325..7759)
                     /locus_tag="PL152_04390"
     CDS             complement(7325..7759)
                     /locus_tag="PL152_04390"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQR48550.1"
                     /translation="MHPYPHFYIVQASATPEGDVPVSSPGLPTLATAPPAEFDGPGDR
                     WSPETLLCAAVADCYLLSFRGVARAGKIAWKSLGCSVRGTLERVEGKTRFTRIEVEAA
                     LVVPEGTDHEKAVQAMERAEKVCLISNSLVAERHLAATVTVG"
     gene            complement(7873..8334)
                     /gene="rlmH"
                     /locus_tag="PL152_04395"
     CDS             complement(7873..8334)
                     /gene="rlmH"
                     /locus_tag="PL152_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017411988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HQR48551.1"
                     /translation="MRVLAAGTRMPAWVDAGFAEYANRLRGDYRLELLEIALGQRGRG
                     DPRRAVAEEGERMLAAAGERAHLVALQVGGKSLSTEQLARWLEDRAGEGQPLAFCIGG
                     PDGLSPDVDARARLRWSLSALTLPHGLARVVVAEALYRAVSVIKGLPYHRA"
     gene            complement(8354..8719)
                     /gene="rsfS"
                     /locus_tag="PL152_04400"
     CDS             complement(8354..8719)
                     /gene="rsfS"
                     /locus_tag="PL152_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HQR48552.1"
                     /translation="MKRPRKKAARAAAKPTLREVVLDALADMKAVDVKALDVRGLTDI
                     TDTMVVASGTSDRHVKSIADRVVQRCKEAGFRPYGMEGERDGEWVLLDLNDVVLHVML
                     PRTRQFYSLEKLWEAGAPA"
     gene            complement(8785..9432)
                     /gene="nadD"
                     /locus_tag="PL152_04405"
     CDS             complement(8785..9432)
                     /gene="nadD"
                     /locus_tag="PL152_04405"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HQR48553.1"
                     /translation="MSPIGVFGGTFDPIHFGHLRTAFELQQVLGLSELRFVPAGNPPH
                     RDVPLADAALRLAMVKAAIADQPGFTVDDREVRREGPSYTVVTLGELRAEAPRRPICL
                     VVGMDAFLGLHLWHRWRDLLALAHVAVAHRPGWRAPTDGALGALLAERGTSRVTDLHE
                     QDAGRIFVHPVTQLEISSTDLRNIIVSGRDPRYLVPDAVRDIIRETGCYSRRTTR"
     gene            complement(9429..10439)
                     /gene="holA"
                     /locus_tag="PL152_04410"
     CDS             complement(9429..10439)
                     /gene="holA"
                     /locus_tag="PL152_04410"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HQR48554.1"
                     /translation="MRTSPDALVASLGRGLRGAYLVTGAEPLLIAEACTAIRHAAREA
                     GYGDREVHFVERGFDWDALLASSANLSLFASRRLVEIKFAAALDAEAQRVLATLAEHP
                     PVDTLLLVAGELDRKPLGAAWVKSFEQHGVLVVTQPVERNALPAWIRERLARNGVRIE
                     PDAAQLLADRVEGNLLAAQQEVERIALLMPASSLGVEDVAQLVADSARFDVFELSAAT
                     FAGQAERALRILAGLRAEGREPPLVLWALVNDLRALSRVTQRMAVERSLDSAFRAEQV
                     WSNRQGPIKAALSRLDRPRIDALLAAAARADRVAKGSLRGDAWVEIERLVARIAGLKL
                     AA"
     gene            10596..11672
                     /gene="pip"
                     /locus_tag="PL152_04415"
     CDS             10596..11672
                     /gene="pip"
                     /locus_tag="PL152_04415"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HQR48555.1"
                     /translation="MSTRRTNAARRKHYPPIEPYRSGWLRVSPVHEIYWEECGNPRGK
                     PAVFVHGGPGAGCGAESRRFFDPRRYRIVLFDQRGSGRSRPHANLEDNTTWHLVADME
                     RLRRFLGIERWLVFGGSWGSTLALAYAEKHPSRVTELVLRGIFMLRKWELDWFYQDGA
                     GAIFPDRWEAYLAAIPKRERGDLVGAYYRRLTSPHRATRVRAARAWSIWEAATSYLHV
                     NEANIAHWGEEDFAVAVARIECHYFVNKGFMRSEDQLLRGVNRIRHIPTVIVQGRYDV
                     VCPMRTAWDLHRRWPEADFRVVPDAGHSAHEAGNTHELVSATDRFANARARPAGATRG
                     RRGRRPSPGAGSPPRGSSRAGRAR"
     gene            complement(11566..12051)
                     /gene="lptE"
                     /locus_tag="PL152_04420"
     CDS             complement(11566..12051)
                     /gene="lptE"
                     /locus_tag="PL152_04420"
                     /inference="COORDINATES: protein motif:HMM:NF016290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HQR48556.1"
                     /translation="MSRAGILAGILAAVTAAGCGFQLKGALALPENVKSVYVATSDEL
                     SPFAVELGRALRAAGATQAPSAAEADAVVRVAQDRTGRRVLSVTGRDTPQEYQVFYVI
                     GYSIDRGDKQAVPPQEIELTRSYTFSEPDLLAKNREEAILRQAMAGDLADLVLRRLAS
                     L"
     gene            complement(12054..>13123)
                     /locus_tag="PL152_04425"
     CDS             complement(12054..>13123)
                     /locus_tag="PL152_04425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQR48557.1"
                     /translation="SPGNDRAMVDDRAAYWMPVDQYIGGIEHAILHLLYSRFWTRVMR
                     DLGLTAIEEPFTNLLTQGMVLNEIYFRKPASGRVVYYNPAEVDVRLGENGARIGAVLR
                     EDGQPVESGGIGTMSKSKGNGVDPQALVEQYGADTARLFMMFAAPPEQSLEWSDEGVE
                     GAFRFMKRLWKAVHQHVGGGTAGPVDVAALTTAQRDLRRQVHETLAKVTVDIGRRRTF
                     NTAIAAVMELMNSVARFEDGSPQGRAVVQEALETVVLVLSPVVPHACHVLWRELGHAG
                     AVVDERWPQVDPKALERDTIEVVVQVNGKLRGRVAVPADADEAAVREAALADVNVQRF
                     MEGKPVRKFVYVRGKLANVVV"
BASE COUNT         1942 a         4775 c         4513 g         1893 t
ORIGIN      
        1 gcgactcgac cggatccatc ggcaggtaca ggccgcgccc gccgcgcgcg tgccacgcct
       61 gcacggaacc acccgacccg gaccttgcga tggcccgcgc agcatgggcg gcttccagca
      121 tcgcggggag cacgacttcg tcggaatacg cgaacccggt tttttctccg gcgagtgcgc
      181 gcacaccgac gccctggtcg atgctgtgcg cgccgtcctt gacgatgccg tcttcgagcg
      241 cccaggactc ctcgcggatc agctggaagt acgcatccgc gtagtccacc gcgtgtccca
      301 tgacctcgcc caggacgccg gcgaccctgc tctcgtcgag cccggccggc gcgagcagcg
      361 cgccgcgcgc aagcgcgagc ggatcccgcg cgagtcccga cgggaccagg ggctggcgtg
      421 gcgcgaacag tgccgggggg atgtcgggtc tcatgcggtt caggcctgca gtcgccgatg
      481 ggcgacggag gggaaattct gcctgacctc gcgctgccgc acgaggtcca ggtcggccgc
      541 cacgaccccg gtgccccgtg gcaggcgggc gagcacgcgc ccccagcagt ccacgatcat
      601 cgagtcgccg tgggtctcgc gcccgttctc gtggaagccg gactgcgcgg gcgccaccat
      661 gtggcagagg ttctcgatcg cacgggcgcg cagcagcgtc tcccagtgcg cccggccggt
      721 cggcacggtg aacgcggagg gcaggcagaa ccactcggcc cccatctcga ggaggcggcg
      781 aaacagctcg ggaaagcgga tgtcgtaaca gacggcgagc ccgacgcggc cggccggcgt
      841 gtccacgcac acacaggcgt cgcccggtcg cacggacgag gactcctggt agcgctcgtc
      901 ccggcccggg acctcgacgt cgaagaggtg gatcttgtcg tagcgcgcga cctgggtgcc
      961 ccgcgcgtcg tacaccaggg aggcggccgc gacgcgcggc tgccccgcga cgcgcaacgg
     1021 gatcgtgccc gcgatgaccc agaggcggag ctcgcgcgcg aggccggaca ggaactgctg
     1081 gatgtcgccg tggccctcgg tctcggcgat cgacagcttg tccgcctcgg ccatgcccat
     1141 gaacgcgaag ttctccggca gggccgccac cgccgcgccg gccgagctcg cttcgcgcag
     1201 cagccgttcg gcatcggcga ggttgcgcct cacgtccggc gtggacgtca tctggatcgc
     1261 ggccacgacc gtcattgctt cacgtcctgt tccggcggcg gggcggtcgt cggggcctcg
     1321 ccgacctgcg acgagacacg ctccacgacc gggtccgccc agggtcccgt gacgtgatag
     1381 taaacgcgcg ccagcccctg cagctgcccc ttgaacagct gcgagatcag gaacccgccg
     1441 gccgcgccga tgggaccgcc ggccagcgcc cctgcgacgg tcgccggccc cgccgggttg
     1501 ccgctcacca ccaccatctg gtcgtaatcc tcggtcgcga gcccggtgcg gcccgcgatg
     1561 ccgatgtcga ccgcgggacc cttgagcagc aggttctgcg tgtacgcgtt gccgtcgcgg
     1621 atctcgaaat cgccgcgcac cgagtcgaat gcgaggccag cgtcggttac atcgtggaaa
     1681 tcgagggaca gtcgccgggg cagctcggcc aggctcgtga ggcccagcat gcgacccgcg
     1741 ccgggcttca cgttgcgcag ctggccgcgg tcgagcgaga tgccgagcgt gccgttcagg
     1801 tgggccagca ccgagcccgt cggtccgtcg ggccaggaca cgctcgcccg cgcggtcgcg
     1861 cgctcggctt ccagcgctcc cgggataccc agcgccttcg acgtcgcccc gagattgctg
     1921 ctccggagtt cgagcccgag gcgagtgcgg gagcccgagc cctcggccag ccactcgccc
     1981 tgcgccgtga tcgtggtgtc agccgtcgtc gccgtcgcac cagataaact cagccccatc
     2041 gcctccttcg tgacactggc cacgaggtgc ccgaagcggc gttcccccca ggcgaaatcg
     2101 ccgatgtcca tgtccagcgc cgggaccttg cgcgggtcaa cggtggcact cgcctgagat
     2161 cccgactccg cggctggacg caggtgcagg cggtccatgg cgaagcggat cggcgagcca
     2221 ccgtgcaggt cgtcgggaat cgtcacgtcg ccttcggcaa gaggcccgct cacctgcacc
     2281 tgcagtgccg ccggctgcgg cttcagtgtg gcgtccagct gctcgaattc gaagccgaag
     2341 atgcgcgcct tgtcgacgtg cacctccgtg ggaccgagcc attgcgagat ggactcgccg
     2401 cccttgccgc cggtgtccag cgccagccac tcgcccaggt cgaacgaagg ccagtcaccg
     2461 gcgaagcgca gtcccggccg ctcgggcatg acgagcggtc gcccgtcgaa gcgcgcgatg
     2521 ccccgatcga gtcgcacccg cccgtcggcc ccctgcgtga gctgcagtcg cacgcgcgcc
     2581 gggcccgact cgaccgcgat ctcgctcagt ccagacggcg caaaggtcag tccgacgtgc
     2641 gtcggtcgcg cgtcggacgg cgccttcggg aacggctccg gagcggacac cccgaggccg
     2701 acgaggtcac tcgcgatgtc gtagcgcgac gtccattgct ccgtgccgcg gtgacgctcc
     2761 atgcgtccct cgaatttcca gtcggcggcg ccggacatgc ggatgccggg cggcaggccg
     2821 atgaatgccg gcaggccggc gcccacgctg tgcccgcggc cgtgcatcag caccgacgcg
     2881 tcgacgtcgc gcgtcagcgg ccccggctcg acgtcgagct cgaacggccc gtccagcatc
     2941 gagccgcgca gcgtgtgcgc gcgcatctcg aggttgtgca cctcgaacgt cccggtcacg
     3001 cgttccgcgg gcgcgcgcag cgccgggatg ccgacggttg ccgagcgcag gttggtccgg
     3061 atcacgaggt cgtgcggctc gtccgggagg gtgggaaggt gcagcttcac ggagtagtcg
     3121 gccggtccct gcccggtgag tcccatgaac tgtccgccga gcactgcgcc caccggactg
     3181 cgctgcacga atggcagcgc acgcgcgagg tcgcccgacg ccgtggcgtc gacctcgacg
     3241 acggcctcgt gcatgtccgc gatgccgacc tcggcgtggt cgagacggag cccgccgacc
     3301 tcgccgccgt gcatgcgcgc gacgaatcct gcgttgtgga actcgacgat gccctgccct
     3361 ttttccagcg gcgcgaagcc ggcgaagtaa tcgaccccga cgccgctcaa ggacgccgtc
     3421 gctgtgaaat cgccctcgcc gttcttgaac ggaaagtcgt gcacccgacc ccggtagctc
     3481 acgtgccccg cgtcgacctg gccggcgagg aaggccggcg cgagccagga gacggcaccc
     3541 gagccgaagc tgccataggg cagcacccgg cccgccaacg cgacctcggc gtgatccacc
     3601 tggatctcca tgtcgaggaa cggcgaaatg ccgggacgct cgtaccggta ctcaaagctg
     3661 ccgtgcgccc gcgcgccggg atgcaccacc gcgacgttgc ccgagccaat gacccaggca
     3721 tcaccgtccc gcttccagtc gacgtcggcc gatacggcga cgccggcgat cggctcgcgg
     3781 aacatcctgg gcaggtcgaa cgtgaatgcc tgcgaacgca atgacagccg cccaccctgg
     3841 tcggtgcccg acatcacgcc ggtgaatccg gcgatcccgg gtgccgtccc ggacgccgag
     3901 aacccgacgc cctcgacatc ggcgctcatt gcgaagcgcg gcgaagcccc ggcacggtcg
     3961 cgcgacgcct cgatgcggaa cgtgtggatt tcgcccgtcg ggttgagtcc ggcccaccgg
     4021 tccagcgcat gaggggcaaa cgccagcgcg agtggccata cgtcgtggat tcgcaggtcc
     4081 gtcgccgaca gcgcggcgtc gaaggtcgtc gccaggtcgc cgcgccagcg cacggagaaa
     4141 ctggtgggaa cgacgcccgg gcgcccggac gggtagagca ggagcttctg tgccctgatc
     4201 gtccacgtct ccccgtcgtg gcgcagtcga accgacccgc gcagattcgg gtactgcacc
     4261 tgtgtcgggc ccgtgtcggc gggacgatcg gcgacggtcg acgtgacgtg cggcagggtc
     4321 atcggcgacg cgccggccgg tcgctccgga gccgacaagg tcagcgtgct cacgggcggc
     4381 gcggcgcgcg gcggcaacgc gagcgacacg tcctgcagcg ccaggtcgag gcgcgcgtgt
     4441 gtcagctggc cgccggcgaa atggatcatc gcgtccaccg cgcccgctcc ggccagcggc
     4501 cgcgcaatcg tggccggaag gaatggcgac aggccggcca gtactagacg atccatccgc
     4561 aactcgaggc cagcgtccaa tgcgtcaaaa tgttcaagag accccttgag gtccccgtcg
     4621 aattcgatcg cgtgtcccag cgagtcgggc aagcgcgccg caccggtcag cgccgtgctg
     4681 tcgtgctggc ggcgcagctc gaagtgcagc tgttccagcg tcacgggcgc ggccctggtc
     4741 accaggtcgc ggtaggtcac cgtcgcgtcc gtgacttcga cgcgaccggc cggcagccgg
     4801 tcgaggtcga acgggggacg atccagtggc cgctcgttct gtccgagcaa ccggatgcgc
     4861 ccatcctcga gtcgcaccac cgtcagcgtc ggccccgtga agcgcacgcg gccggccacg
     4921 agctcgccgg tgcggaacag gctccagagg tccagtccga cgctgccttc acgcgaacgg
     4981 aacagcgcct gcgagccgtc cttctgcagg atgcggacgc cgcgcagcac gacctccggc
     5041 ccgaaccagc gcaggcgcgc gtcgagggcg tcgtactcga cccgcaggtc cgtctgccgc
     5101 tcgacccagg cctgcaggcg ggcggcgttc tcgggcagcc gggcgattgc aagcctgagc
     5161 gcgccgatgg cgagcgccgc cgtgatcagc aggcccgcga cgatcgccgc ggtcacacgc
     5221 cagatccggg cggcccgccc acgcccggcc gcggcgttca catgagcacc acgtcgtact
     5281 ggtcgagcat gtagagcgat tccgtctgca ggcggatcgg cttgccgagc tgcacctcca
     5341 gctcgccgag cgcggccgac tcctcgtcga gcagccgctc gatgacgtcc tggtgcgcga
     5401 gcacgagcag ctgctgcagg tcgaactgcg cggcctgccg caggatctcc cggaagatct
     5461 cgtagcagac ggtctcgggc gtcttcacga agctgcggcc ctcgcaggcc gggcacggca
     5521 tgcacagctg gtgcacgagg ctctcgcgcg tgcgcttgcg cgtcatctcg acgaggccca
     5581 ggggcgacac tgccgagaag tgcgtcttcg cgtggtcctt ggcgagcgcc ttctcgagcg
     5641 cctgcaggac ctggcggcga tgctcctcct cctccatgtc gatgaagtcg atgatgatga
     5701 tgccgccgag gttgcgcagc ctgagctgcc gcgcgatcgc caccgccgcc tcgagattgg
     5761 tgcggtagat cgtctcctcg aggttgcggt ggccgacgta cgcgccggtg ttgacgtcga
     5821 tggtggtgag cgcctcggtc tggtcgatga tcaggtagcc gcccgacttg agcgccacct
     5881 tgcggtcgag cgagcgctgg atctcctcct ccacgccgtg caggtcgaag atcggccgga
     5941 cgcccgtgta gcgctcgacg cgcgtctcga gtcccggcat gaacgtcgcc gcgaactcgc
     6001 gcatgcgatc gcaagtgcgc tcgttgtcca ccagcacgcg gtccacgcct tcgccgacga
     6061 ggtcgcgcat caggcgcagc gcgagcggca ggtcctcgtg cacgagcgtg ccctgcccgg
     6121 ccgcgcgcgc cttgtccgtg agcacctccc acagcttgcg caggaacagc cggtcggccc
     6181 tgagcgcctc cgccgcggcg ccttccgcag ccgtgcgcac gatgtatccg cccgtgtcgc
     6241 ccggcgcggc ggcggcgttc accgcgtcgc gcaatcgctg gcgctcctcc tcgttctcga
     6301 tgcgcgccga cacgccgacg ccgcgcccga agggcatgta gacgaggtag cgcgaaggaa
     6361 tggtgatgaa cgtagtcagc cgcgcgccct tggtgccgag cggatccttc agcacctgca
     6421 cgaggatgtc ggaaccctcg cccacgagat cccggatgtt gtcggtgcgc ggcgcatccc
     6481 cggaaccctc ggcatccgcg ggctgcacga tgtccgacac gtgcaggaac gccgtgcgct
     6541 cgaggccgat gtccatgaag gcggcctgca tgccgggcag gacgcgcgac acgcgaccct
     6601 tgtacaggtt gcccgtgagc ccgcgcttgc tcgcccgctc gacgaacagt tcctgcagca
     6661 cgccgttctc gagcaacgcg gcgcgcgtct cgcggggact gacgttgacg accatctcgg
     6721 tgctcactgg gcgcctccag cgatgagacc cggcacggcg gtgagcagtt cccaggtctc
     6781 gcagagcggc agacccatca cgccggagta gctgccggaa atgtgcgtga tgaagattgc
     6841 cgcgcgtccc tgcaccgcgt aggcgccggc cttgtcggcc ggctcgccgg tgctccagta
     6901 ccagtcgagc tcggccggcg tgatcgcgcg catcgtcacc tggctggtgc tcagccgcga
     6961 cgcgctgccg gacgcgtgca gaaccgccac ggccgtgtgc acttcgtgcg tgcggcccgc
     7021 gagccgcttc agcatcgcga gcgcctcgtc gcgatcggcc ggtttgccga agatctcgcc
     7081 gtcgagttcg accgtcgtgt ccgcggcgag caccggcagg cggcgcgcat cggtgccgcg
     7141 cgaccagagc gcccgggctt ttgcctccgc aagccgtcgc acgtacgcgg ccggcgcctc
     7201 gcccgccagc agcgactcgt cgacctccgc tggccgcgct tcgtgcgcga cgccgatctg
     7261 ccgcagcagt tcgctgcgcc tcggcgaggc ggaggcgagg cagatgaccg gccgctgcac
     7321 ggactcagcc cacggtgacc gtcgccgcga ggtgacgctc ggccaccagc gagttgctga
     7381 tgaggcagac cttctccgca cgctccatcg cctgtaccgc cttctcgtga tcggtgccct
     7441 cgggcacgac gagcgcggcc tcgacctcga tgcgggtgaa ccgggtcttg ccctcgaccc
     7501 gctccagggt gccgcgcacc gagcatccta gcgacttcca cgcaatcttc ccggcccgcg
     7561 cgacaccgcg aaaactgagc aggtagcagt ccgcgacggc cgcgcagagc agcgtctccg
     7621 gcgaccaccg gtcgcccggc ccgtcgaatt cggcgggcgg cgccgtagca agtgtcggca
     7681 ggcccgggga ggacaccggc acgtcgcctt cgggcgtcgc gctggcctgg acgatgtaga
     7741 agtgaggata cggatgcatg cgggtctcgc gaaagctcga gaatcctgca ttcaaacccg
     7801 ccggcccggc cgctgtccac cgggcatgcc gcaatccagc ggcgggcccg gtgcattgcc
     7861 ccgtgcaact cgtcaggcgc gatggtacgg caggcccttg atgacgctca cggcccggta
     7921 cagggcctcg gcgaccacga ccctggcgag accgtgcggg agcgtgagtg ccgacaacga
     7981 ccagcgcagc cgcgcccggg catcgacgtc gggggacagt ccgtcggggc cgccgatgca
     8041 gaacgcgagg ggttgtccct cacccgcgcg gtcctcgagc cagcgcgcca gctgctcggt
     8101 cgagagcgac ttgccgccga cctgcagggc gacgaggtgc gcccgttcac ccgcagccgc
     8161 gagcatgcgt tcgccttcct ccgccacggc gcgccgcgga tcgccgcgcc cgcgctggcc
     8221 gagcgcaatc tcgaggagct cgagccggta gtcaccacgc aggcggttcg cgtactcggc
     8281 gaacccggcg tccacccacg ccggcatccg cgtgccggcg gcgagcactc gcatcagcat
     8341 cgcggtcggt gcgtcaggcc ggggcgccgg cctcccagag cttctcgagc gaatagaact
     8401 ggcgcgtgcg cggcagcatc acgtggagca cgacgtcgtt caggtcgagc agcacccact
     8461 cgccgtcccg ttcgccctcc atgccgtacg ggcggaatcc cgcctccttg cagcgctgca
     8521 cgacgcggtc ggcgatcgac ttgacgtgcc ggtccgacgt gccgctcgcg acgaccatcg
     8581 tgtccgtgat gtcggtcagg ccacggacgt cgagcgcctt cacgtccacg gccttcatgt
     8641 cggcgagcgc gtcgagcacg acctccctga gcgtcggctt cgccgccgcg cgagccgcct
     8701 tcttacgcgg ccgcttcacc ggtccggcgg tcgaactcgc tgccctggtc gtggatcgtg
     8761 tcttcgctcg cttcgctgtc atgctcacct cgtggtcctg cgggagtaac aacctgtctc
     8821 gcggatgatg tcgcgcacgg cgtcgggcac caggtagcgc gggtcgcgtc cgctcacgat
     8881 gatgttgcgc aaatcggtcg aggagatctc gagctgcgtg accgggtgca cgaagatgcg
     8941 gccggcgtcc tgttcgtgca ggtcggtgac gcgactcgtg ccacgctcgg ccagcagtgc
     9001 gccgagcgca ccgtccgtcg gcgctcgcca gcccgggcgg tgcgcgacgg ccacgtgcgc
     9061 gagcgccagc aggtcccgcc agcggtgcca caggtgcagg ccgaggaatg cgtccatccc
     9121 gacgaccagg cagatgggcc gccgcggcgc ctcggcgcgc agttcgccga gcgtcaccac
     9181 cgtgtacgag ggcccctcgc ggcgcacttc gcgatcgtcc accgtgaatc cgggctggtc
     9241 ggcgatcgcg gccttgacca tcgcgagcct gagcgccgcg tccgccagcg gcacgtcgcg
     9301 gtgcggcggg ttgcccgccg gcacgaagcg cagttccgac aggccgagca cctgctgcag
     9361 ctcgaacgcc gtccgcaggt ggccgaagtg gatcgggtcg aaggtgccgc cgaagacgcc
     9421 gatggggctc atgcagccag cttgaggccg gcgatcctcg cgacgagccg ctcgatctcg
     9481 acccaggcgt ccccgcgcag gctgcccttg gcgacacggt cggcccgcgc ggcggccgcg
     9541 agcaacgcat cgatgcgcgg gcggtcgagg cgcgacaacg cggccttgat cgggccctgg
     9601 cgattggacc acacctgctc ggcgcgaaac gcactgtcca gcgagcgctc gaccgccatg
     9661 cgctgcgtga cgcgcgacag cgcacgcagg tcgttcacca gcgcccagag caccagcggc
     9721 ggctcccgtc cctcggcgcg caacccggcg aggatgcgca gggcgcgctc ggcctgcccc
     9781 gcgaacgtgg ccgcggacag ctcgaacacg tcgaatcggg cgctgtcggc gacgagctgc
     9841 gcgacgtcct ccacgcccag cgacgacgct ggcatgagca gcgcgatgcg ttcgacctcc
     9901 tgctgcgcgg cgagcaggtt gccctccaca cggtcggcca gcagctgcgc tgcgtctggc
     9961 tcgatgcgca cgccgttgcg ggcgagacgc tcgcgaatcc acgctggcag cgcattgcgc
    10021 tcgacgggct gcgtcacgac gagcacgccg tgctgctcga acgacttgac ccaggcggcg
    10081 ccgagcggct tgcggtccag ttcgcccgcg accagcagca gcgtgtcgac cggcgggtgc
    10141 tcggcgaggg tcgcgagcac gcgctgcgcc tcggcatcca gggccgcggc gaacttgatc
    10201 tcgacgagac ggcgcgacgc gaacagcgaa aggttggcgg aactcgcgag cagcgcgtcc
    10261 cagtcgaagc cgcgctcgac gaagtgcacc tcgcgatcac cgtagccggc ttcccgcgcg
    10321 gcgtgacgaa tggcagtgca cgcctctgcg atcagcagcg gctcggctcc ggtcacgagg
    10381 tatgcgccac gcagcccgcg cccgaggctt gcgaccagcg cgtcgggact cgtcctcact
    10441 gcccggggcg gctcatgcgc tcgaagcggc ggcgcaaccg gtggctggct taggcatgcg
    10501 ccccgattat gcagcgttgg cctgcggcat gccaacggca gcaccggggc gcggcctacc
    10561 cccaccccac cctgccgcgc ggctagaatc cgcgcatgag cacccgtcga actaacgcgg
    10621 cacgccgcaa gcattacccc ccgatcgagc cctatcgcag cggctggctg cgcgtctcgc
    10681 cggtccacga gatctactgg gaggaatgcg gcaacccgcg gggcaagccg gccgtgttcg
    10741 tgcacggcgg cccgggcgcc ggctgcggcg ccgagtcgcg gcgcttcttc gatccccggc
    10801 gctaccgcat cgtgctgttc gaccagcgcg gcagcgggcg cagccggccg cacgccaacc
    10861 tcgaggacaa cacgacctgg cacctggtgg ccgacatgga gcgcctgcgc cggttcctcg
    10921 gtatcgagcg ctggctggtg ttcggcggat cctggggctc gaccctggcc ctcgcctacg
    10981 ctgagaagca tccctcgcgc gtcacggaac tggtgctgcg cggcatcttc atgctgcgca
    11041 agtgggagct cgactggttc taccaggacg gcgcgggggc gatattcccc gaccgctggg
    11101 aggcgtacct cgcggcgatc ccgaagcgcg agcgcggcga cctcgtgggc gcgtactatc
    11161 gccgcctcac gagcccgcat cgagccaccc gcgtgcgcgc ggcgcgggcc tggtccatct
    11221 gggaggcggc aacgagctac ctgcacgtca acgaggcgaa catcgcgcac tggggcgagg
    11281 aagacttcgc ggtcgccgtc gcccgcatcg agtgccacta cttcgtcaac aagggattca
    11341 tgcgcagcga ggaccagttg ctgcgcggcg tcaaccggat ccggcacatt cctaccgtga
    11401 tcgtgcaggg ccgctacgac gtggtctgcc cgatgcgcac cgcctgggac ctgcatcgtc
    11461 gctggcccga ggccgacttc cgcgtcgtgc ccgacgccgg gcattcggcg cacgaggccg
    11521 gaaacacgca cgagctcgtc agcgcgaccg accgtttcgc gaatgctaga gcgaggccag
    11581 ccggcgcaac acgaggtcgg cgaggtcgcc ggccatcgcc tggcgcagga tcgcctcctc
    11641 gcggttcttc gcgagcaggt cgggctcgct gaaggtgtag ctgcgcgtga gttcgatctc
    11701 ctgcggcggc accgcctgct tgtcgcctcg atcgatggaa tacccgatca cgtagaagac
    11761 ctggtactcc tgcggggtgt cccgtccggt gaccgacagc acacgacggc ccgtgcggtc
    11821 ctgcgcgacg cggacgactg catccgcctc ggccgccgac ggggcctgcg tcgcgccggc
    11881 ggcacgcagc gcgcgcccga gttccaccgc gaatggcgac agctcgtcgc tcgttgcgac
    11941 gtacacggac ttcacgttct caggcagcgc gagcgcgccc ttcaactgga agccgcagcc
    12001 agcggcggtc accgctgcaa ggattccggc gagaattccc gcacgcgaca cgttcagacc
    12061 accacgttgg cgagcttgcc gcgcacgtag acgaacttgc gcacgggctt gccttccatg
    12121 aaccgctgaa cattgacgtc cgcgagagct gcctcgcgca cggccgcctc gtccgcgtcg
    12181 gccgggaccg cgacacggcc acgcagcttg ccgttgacct gcaccacgac ctcgatggta
    12241 tcgcgctcga gcgccttcgg gtcgacctgc ggccagcgct cgtcaacgac cgcgcccgcg
    12301 tgcccgagtt cgcgccacag cacgtggcag gcgtgcggga cgaccggcga cagcaccagc
    12361 accacggtct cgagtgcctc ctgcaccacg gcccggccct gcggcgagcc gtcctcgaag
    12421 cgcgctaccg agttcatcag ttccatgacg gcggcaatgg cggtgttgaa cgtgcgccgg
    12481 cggccgatgt ccacggtcac cttcgcgagc gtctcgtgca cctgccggcg caggtcgcgc
    12541 tgcgcggtcg tgagggcggc cacgtcgacg ggcccggcgg tcccgccgcc gacgtgctgg
    12601 tgcacggcct tccacaggcg cttcatgaag cggaaggcgc cttcgacgcc ttcgtcggac
    12661 cattcgagcg actgttccgg cggagcggcg aacatcatga acaggcgcgc cgtatccgcc
    12721 ccgtactgct cgaccagcgc ctgcggatcg acgccgttgc ccttcgactt cgacatcgtg
    12781 ccgatgccgc cggactccac cggctggccg tcctcgcgca gcaccgcgcc gatccgcgcg
    12841 ccgttttcac ccaaccggac gtcgacctcc gctgggttgt agtacacgac gcgcccggac
    12901 gcgggtttgc ggaagtagat ctcgttcagc accatgccct gcgtgagcag gttcgtgaac
    12961 ggctcctcga tcgcggtgag gccgaggtcg cgcatgacgc gcgtccagaa ccgcgagtag
    13021 agcaggtgca ggatcgcgtg ctcgatgccg ccgatgtact ggtccaccgg catccagtac
    13081 gcggcgcggt cgtccaccat cgcccggtcg ttgcccggac tcg
//
LOCUS       DAOMFD010000047        13014 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1171_length_13014_cov_27.652442, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000047 DAOMFD010000000
VERSION     DAOMFD010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 13014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13014
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1171_length_13014_cov_27.652442"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1304
                     /locus_tag="PL152_04430"
     CDS             <1..1304
                     /locus_tag="PL152_04430"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HQR48558.1"
                     /translation="AFPDRYPVLLESVGGGLPLGRYDVLLCLPGEQLVQARNGRLRGP
                     SGRGHERFLDALDDWWQVDRESRPTVTGTPFAGGWFLYAGYELADEIEPTLRLPRSRH
                     VRAVAWRMRGAVVRDRATGALGFHTEDGRADALQRQVSADLARLVEPAPFPQALVEGA
                     VDEEDPREFTSGVAEVLDAIARGDVYQANLSRGWSARLAQGATAAHVYARLRAANPAP
                     FAGIAALPGFSLLSSSPERLLAVRDGVASTRPIAGTRPRGRTSAADASLRQELVLNEK
                     ERAEHVMLVDLERNDLGRICKGGTVRVDEYMTVESYATVHHIVSNVRGDLRDGVTPGQ
                     AIAAVFPGGTITGCPKIRCMQLLAGLERAPRDAYTGSMGYLGLDGSLDLNILIRTLSL
                     AGDRIDFRTGAGIVADSQPEAELAETRAKARGMLRALEGRE"
     gene            1301..2107
                     /gene="pabC"
                     /locus_tag="PL152_04435"
     CDS             1301..2107
                     /gene="pabC"
                     /locus_tag="PL152_04435"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR03461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008696 - 4-amino-4-deoxychorismate lyase
                     activity [Evidence IEA];
                     GO_process: GO:0008153 - para-aminobenzoic acid
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HQR48559.1"
                     /translation="MIGRRLVNGSETAGIAADDRGLQYGDGLFETMAATGGRIRHLEL
                     HLARLEEGCRRLRMPMPAAGLVEDECARVLEGLGTGTVKLTLTRGPGPRGYRPPPEPS
                     LTRIVVSSAARPRGDALPPLTVRICETRMGLNPALAGLKHLNRLEQVMACAEWDDPAI
                     GEGLMLASDGRLVSATAANVFLVEAGRLVTPDIRDCGVAGVMRQVVLTAAAELGLPHA
                     IEDVHPDRLQFAEEVFVTNAVVGIRPVAELVGGRSWEAGEVTRVLMAHTA"
     gene            2104..3144
                     /gene="mltG"
                     /locus_tag="PL152_04440"
     CDS             2104..3144
                     /gene="mltG"
                     /locus_tag="PL152_04440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HQR48560.1"
                     /translation="MTRGRTIALVLAGLVAASALAGYAGWVSLERWLDRPMAIPGGEF
                     TVEIPKGRPLARTTQELASLGVLDHPRLLAAYARLTGADARVRAGEYAVPAGTTPRRL
                     LALFESGAVVHHSITLVEGWTFRDLLSALERDPLLVHKLSGQDDAAVMRALGEPGVAP
                     EGLFFPDTYLYDKGTTDVAILQRARQRMRTELASAWDARADDLPIDDAYQALILASIV
                     EKETALSSERPRIAGVFVQRLRLGMRLQTDPSVIYGLGPGFDGNLRKADLERDGPYNT
                     YTRAGLPPTPIAMPGAASLAAAVRPDERGEIYFVATGLPDGSHEFSRTLAEHDRAVAR
                     YLKRYRDQTAGP"
     gene            3145..3780
                     /gene="tmk"
                     /locus_tag="PL152_04445"
     CDS             3145..3780
                     /gene="tmk"
                     /locus_tag="PL152_04445"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HQR48561.1"
                     /translation="MAGRFITFEGGEGVGKSTQIARAADWLRTRGREVVLTREPGGTP
                     RAERLRRILLEHDDEPMPGACELLLMFAARATHLENLVRPAIARGAWVLCDRFTDATY
                     AYQGGGRGVPESRIAALEELVQGGFQPDLTLLLDVAVDVGLARASARNGHRGPDRFEK
                     ERLEFFERVRAAYLERVHRHPGRIRVVDAGGTVDEVERAVRRHLEALPGAA"
     gene            3780..4811
                     /gene="holB"
                     /locus_tag="PL152_04450"
     CDS             3780..4811
                     /gene="holB"
                     /locus_tag="PL152_04450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HQR48562.1"
                     /translation="MTDESADTTPALPLEMLPWHEAARLRLEAAAASGRMPHAVLVHG
                     PSGVGKERFAGAVAAALLCAQRGDRFAACGACPECALVRAGSHPDLHWLRPAAEKKST
                     GVDQVRETCEQLGMTSMRGGFRVAIVAPAQAMTTSAQNALLKTLEEPAARTLLVLVTS
                     RPSALLPTLRSRCQRLEIACPPEQQAAGWLERETGAPVADGLLELAGGAPLRALQIAP
                     HYGALDAQMTELLAGLLAGRSEACAAAADMMGEGLPVRLDWLERWLGRLVRGRAGSNA
                     TRLTVRGGAVLQRAAAEVNISAAFRIVDRVREVRRLLEGSAAPQLLVEALLVDVAAAL
                     GSRGVARWP"
     gene            4802..5158
                     /locus_tag="PL152_04455"
     CDS             4802..5158
                     /locus_tag="PL152_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HQR48563.1"
                     /translation="MAVTRQPNKPGLLTLTIKDKSALYLAYMPFVKNGGLFIPTSSNY
                     RLGDEVFMLLNLMGEDEKLPVAGRVIWMTPKGAQGKRTAGIGVQFSEQDRGNTQRKIE
                     AYLAGALGGDKPTHTM"
     gene            complement(5162..6232)
                     /locus_tag="PL152_04460"
     CDS             complement(5162..6232)
                     /locus_tag="PL152_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HQR48564.1"
                     /translation="MTETSTLDPKLLRSFSPLDGLKNENLRALARKTTLRELGHGRVL
                     FKEGDTDKRTIYLVSGAVDYLTDGRIVGSVKGGTQDARHPLAPILPRRCTARVATDRI
                     EYLAIDSDLLDVLITWDQTGTYEVGDLNAAGSGGDWMTVLLQSRAFHRIPPANLQAVF
                     MRMQRVEFTAGETIVRQGDEGDYFYVIVSGRCAVTRETPLNREGIKLAELGMGDTFGE
                     EALVSGAPRNATVTMLTDGTLMRLGKEDFNSLLNEPMLRWVDYTEARRIVAGGGQWLD
                     VRLPSEYEHCHFDNALNVPLYFIRLKLKSLDTSVPYVVCCDNGRRSSAAAYLLSERGF
                     DTSVLRGGLATTDLADALNAVG"
     gene            complement(6325..7080)
                     /locus_tag="PL152_04465"
     CDS             complement(6325..7080)
                     /locus_tag="PL152_04465"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQR48565.1"
                     /translation="MRFLLYNIRYATGTGAAFHLPVPGAGYLRSNPRVLEGITRFIRS
                     VDPDIVGLVEVDTGSIRSGRVNQAEVIAESLGHYSTYQCKYGTNSLNTLVPIVNKQAN
                     AFLAAPRVEGERFHYFETGIKRLIIELELDELCVFLVHLSLKFRHRQTQLRHLFELVR
                     ASRKPVIVAGDFNTFWGEHEMALFMEAAGLKSANRDRVPSYPARRPRMELDFILHTRD
                     IVIDAFSVADVRYSDHRPLICEFRVEGRQRAAA"
     gene            7183..7644
                     /locus_tag="PL152_04470"
     CDS             7183..7644
                     /locus_tag="PL152_04470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48566.1"
                     /translation="MSSVSQQNPIRLFVCHVWVEDDDYQRVFEYLESAPRFYYKNTST
                     PGDRPSGDREVLRENLRRQIVEAEIVIMPASLYRRHHDWAEFQLHCAKAFDKPVVVLE
                     PFGSHDTIAPAVLELGDEVVPWDLRQIVDAIKRQARHEETTRYDVIEFKLD"
     gene            complement(7656..7991)
                     /locus_tag="PL152_04475"
     CDS             complement(7656..7991)
                     /locus_tag="PL152_04475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48567.1"
                     /translation="MRRLRDAAELLVGPGPVKQRLCAAYLQHLRDMEVDALPDDLAVS
                     YRALESALSTAQPAGGLGAVEVTIRKMSEADASQHASAMLDLYVNVAGRQAEPSPALS
                     RQLRLVGDE"
     gene            complement(8206..9072)
                     /locus_tag="PL152_04480"
     CDS             complement(8206..9072)
                     /locus_tag="PL152_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQR48568.1"
                     /translation="MAGPVWFKVRKDLGHQPIVAGRIPGPTPLFAERYIYQDIERTVS
                     AMNLAAALVVQFGGGRVREGDPSRWRSMNLPTQSLLVPGATATHWHYSGTVDFAVFYF
                     LEPASKSMQSLAMLAGSRGEPVSFGDSLVGAAALQLVGELQKGPGADAVFMEQLAAVM
                     IEQTYRALVTPGTRGINPRHVHFSRLQAVLNHIHANVAADLSAPALAVRAGVSLAHFS
                     RIFRDAVGVPPHRYVLAARLDLARKLLTQSSMPISRVAEECGFSSQSHLTASFRAAHS
                     TTPAEFRAHHRQ"
     gene            9187..10470
                     /locus_tag="PL152_04485"
     CDS             9187..10470
                     /locus_tag="PL152_04485"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48569.1"
                     /translation="MIKLKSKLTLPAVGLTGFELPLTEEEAAVQAGVHRFAKDVLRPL
                     GAELDRMTAEEVIAPGSPYWSVFPEFAKLGLDPAMMSELPPEVAIRLESLILEELSWG
                     DAGLGVTLAVAGFPLEMAASLGNKELVDLCTGRIGCWMITHPDKGSDVQVFDMAREWP
                     AGQPGNKGNMWGKVQGDEIVINGQCSAWVSNGAVAQMALGYIGAEYDGSFFEKDGRPH
                     GMTVFIPLDLPGVSKGKPLDKIGQRSLPQGEIYFDNVRIPKRFGVALRDEYYGNVASV
                     WSFAGTAMAQGFTGVARAAFELALQYCHERKQGGKLLIDHQLTRYRLGDMLRRVELCR
                     AVARRSLAYARLSPQSHPYVTAAGKVTVTQEAMKVVDEALQLFGGAGTSREYPIEKLY
                     RDTRAALIEDGENYVLTMRLGLLAQQLYAEGWAQA"
     gene            10544..11674
                     /locus_tag="PL152_04490"
     CDS             10544..11674
                     /locus_tag="PL152_04490"
                     /inference="COORDINATES: protein motif:HMM:NF039925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5938 domain-containing protein"
                     /protein_id="HQR48570.1"
                     /translation="MAKYPVVVYGASGYTGMLIMDWLIDQKIPFTAVARNAKRTQEMM
                     AQRVVRLESATYEIIEAEHKVDSLTKAFKGAKVVCNTVGPFVNFGLTGVEAALKAGCH
                     HIDTTGEQAYMRAVRDKFGESFRQAGLLVSPSIAYMYTLTEIAAELALETPGIDALET
                     GTLARGPRAGSGVTVGSTASIFEMYRSEACFLWEKELVPHAPGTSASIVDPNFAQPVF
                     SLPWGGTSLPVYFEHDSRVRSCISYVGFYDNNVMQQVHALGQKWEAEYKNLPKEHQDA
                     VLKQIVDSTTPAMPPRERTTLQRSVDFAIGRGQLAAVRATVHGVTPYISTGAVQTAAV
                     LKLLDGETNRVGFASGSKAFGHRYLLGFLEQRGLASASVTKL"
     gene            11740..>13014
                     /locus_tag="PL152_04495"
     CDS             11740..>13014
                     /locus_tag="PL152_04495"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR48571.1"
                     /translation="MAIIDFFDRGWRINPKGAAYIQDDRSYSFDDVGELSCRIANGLL
                     TLGLPKETKGAVWATNDVTAWTCTLGLWRANMCWIPVGARNPAEENHYVLDAFDCEVL
                     FFQKYFAPVIAELQPRLPKIRLWICTDGDAPEVKGATSLQSWVAGLPPTCPRVAVDMN
                     DVVMLSSTGGTTGKPKGVMNTHRSAQTFCAHFMIDCPYEADEAPVNLAAAPMTHTAGL
                     LSIPCTARGGTVVVVTKPDPTILLGAIPKYRVTELFLPPTVIYRLLDIPDLGKKVDFS
                     SLKYFMYGAAPMSVEKLKQAIQVIGPVMAGGYGQTEAPASISYLPPSAHFVDGKLASD
                     ERLSSVGYPNPLIRVEIMNDANEIVPKGETGEICVRGDLVMKGYYKDPVKTGETVIDG
                     WLHTGDIGHLDGEGFLHITDRKKDMIITGGFNV"
BASE COUNT         2140 a         4267 c         4575 g         2032 t
ORIGIN      
        1 cggcattccc ggatcgttat cccgtcctgc tcgaaagcgt cggtggcgga ttgccgctcg
       61 ggcgctacga cgtgctgctc tgcctgcccg gcgagcaact ggtccaggca aggaatggac
      121 gcctgcgcgg gccatctggt cgtggccacg agcgcttcct ggacgcgctg gacgactggt
      181 ggcaggtcga ccgggagtcc cgtcccacgg tgacaggcac gccgttcgcc ggaggctggt
      241 tcctctacgc cggttacgaa ctggccgacg agatcgaacc gacgctgagg ctgccgcggt
      301 cgcggcacgt gcgcgccgtc gcctggcgca tgcgcggagc ggtcgtgcgc gaccgcgcaa
      361 ccggcgccct tggatttcac accgaggacg gccgcgcgga tgcgctgcag cggcaggtca
      421 gtgcggacct cgcgcggctc gtggaaccgg cgccgtttcc gcaggcgctc gtcgaaggtg
      481 ccgtcgacga ggaggaccct cgggaattca ccagcggggt cgccgaggta ctcgacgcga
      541 tagcgcgcgg cgacgtttac caggccaatc tctcgcgtgg gtggtcggct cgcctggccc
      601 agggcgcgac ggcggcgcac gtctacgcaa ggctcagggc cgcgaatccc gctccctttg
      661 ccggcattgc ggcattgccg ggattttcac tgttgagttc ctcgccggag cggttgctgg
      721 ctgtccgcga cggcgtcgcc agcacccgcc cgatcgcggg cacgcggccg cggggccgga
      781 cgtccgcggc cgacgcgtcg ctcaggcagg aactggtcct gaacgagaag gagcgagccg
      841 agcacgtcat gctcgtggac ctggagcgca acgacctcgg ccgcatctgc aagggcggca
      901 cggtgcgcgt cgacgagtac atgacggtcg agtcgtacgc cacggtccac cacatcgtgt
      961 ccaacgtgcg cggcgacctg cgcgacggcg tcacgcccgg acaggcgatc gcggcggtct
     1021 ttccgggcgg cacgatcacg ggatgcccca agatccgctg catgcaattg ctggccgggc
     1081 tggagcgcgc gccccgcgac gcgtatacgg gatccatggg atacctggga ctcgacggct
     1141 cgctggacct caacatcctg atccgcacgc tctcgttggc gggcgaccgc atcgacttcc
     1201 ggacgggcgc cggcatcgtc gcggattcgc agccggaggc ggaactcgcc gagacgcggg
     1261 cgaaggcgcg gggcatgctg cgtgccctgg agggacggga atgatcggac ggcgactggt
     1321 gaatgggtcg gagacggccg gcatcgcggc ggatgaccgt ggcctgcaat acggcgacgg
     1381 attgttcgag acgatggccg ccacgggcgg ccgcatccgg catctcgagc tgcacctggc
     1441 gcgactggag gagggctgcc gaaggctgcg catgccgatg ccggcggccg gcctcgtcga
     1501 ggacgagtgc gcccgcgtgc tcgagggcct gggcaccggc acggtgaagc tcacgctgac
     1561 gcgcggcccg ggaccccgtg gctaccggcc gccccccgag ccgtcgctga ctcgcatcgt
     1621 ggtgtcgtcg gcagctcgcc cacggggcga cgcgctcccg ccgctgacgg tccggatctg
     1681 cgagacgcgc atgggactga acccggcgct cgccggcctg aagcacttga atcgcctcga
     1741 gcaggtcatg gcctgcgcag aatgggacga cccggcgatc ggcgaaggac tgatgctggc
     1801 tagcgacggt cgcctggtct cggccaccgc ggccaacgtg ttcctggtgg aggctgggcg
     1861 cctcgtcacg ccggacatcc gtgattgcgg agtcgccggc gtcatgcgcc aggtggtgct
     1921 gacggcggcg gcggaactcg gcctgccgca cgcgatcgag gacgtgcatc ccgaccgcct
     1981 gcagttcgcc gaggaggtgt tcgtgaccaa cgctgtggtc ggcatccggc ccgtggcgga
     2041 actggtcggc ggacggagct gggaggccgg cgaggtcacc cgggtgctca tggcacacac
     2101 agcgtgacgc gcggccggac cattgcgctc gtcctggcgg gcctggtcgc cgcatctgcg
     2161 ctggccgggt acgcgggctg ggtgtcgctc gagcgctggc tcgaccggcc catggcgatc
     2221 ccgggcggtg agttcacggt cgagataccg aagggccggc cactcgcgcg tacgacccag
     2281 gaactcgcat cgctcggtgt cctggaccac ccgcggctgc tcgcggcgta cgccaggctg
     2341 accggcgcgg acgcgcgcgt gcgcgccggc gagtacgccg tgccggccgg gacgactcct
     2401 cgccggctgc tggcgctgtt cgagtccggt gccgtcgttc accactcgat cacgctcgtc
     2461 gaaggctgga cgttccgcga cctgttgagt gccctcgagc gcgatccgct gctcgtccac
     2521 aagctgtcgg gccaggacga tgcggcggtg atgcgcgccc tcggagaacc gggagtcgcg
     2581 cccgaggggc tgttctttcc ggacacgtac ctgtacgaca agggcacgac ggatgtcgcg
     2641 atcctccagc gcgcgaggca gcgcatgcgc accgagctcg cgtcggcctg ggacgcgcgc
     2701 gccgacgact tgccgatcga cgatgcctac caggcgctga tactcgcatc catcgtcgaa
     2761 aaggagactg cactgtcgtc cgaacggccg cgcatcgccg gcgtcttcgt ccagcgcctg
     2821 cggctcggga tgcgtctgca gacggacccg agcgtgatct acgggctcgg tcccgggttc
     2881 gatggcaacc tgcgcaaggc cgatctcgag cgcgacgggc cgtacaatac gtacacgcgg
     2941 gccggactgc cgccgacgcc gatcgcaatg ccgggagcgg cgtcgctcgc agcggcggtc
     3001 aggcccgacg agcgcggcga gatctatttc gtggcgacgg gcctgccgga cggcagccac
     3061 gagttctcgc ggacgctcgc ggagcacgac cgggcggtgg cgcgttactt gaagcgatac
     3121 cgggaccaga cagcggggcc gtgaatggcg ggcaggttca tcaccttcga gggcggggag
     3181 ggcgtcggga agtcgacgca gatcgcacgc gccgcggact ggctgcgcac gcgcggccgg
     3241 gaggtcgtcc tgacccgtga accgggcggg acgccgcgcg cggagcggtt gcgccgcatc
     3301 ctgctcgagc acgatgacga gccgatgccc ggtgcgtgcg agctgctgct catgttcgct
     3361 gcccgcgcca cgcacctcga gaacctcgtg cgccccgcga tcgcccgcgg cgcgtgggtg
     3421 ctctgcgacc gcttcaccga cgcgacctac gcctaccagg gcggcggccg cggcgtcccg
     3481 gagtcacgca tcgccgcact cgaggaactc gtgcagggcg gattccagcc ggacctgacg
     3541 ctgctgctcg acgtcgcggt cgacgtcgga ctggcgcgcg ccagcgcccg caacgggcat
     3601 cgtggtccgg accgcttcga gaaggaacga ctcgagttct tcgagcgcgt gcgggccgcc
     3661 tatctggagc gagtgcatcg tcatcccggg cggatccggg tcgtcgatgc aggcggcacg
     3721 gtcgacgagg tcgagcgcgc cgtccggcgc catctcgagg ccctgccggg agccgcgtga
     3781 tgaccgacga atccgcggac accacgcctg cgctgccgct cgagatgctg ccgtggcacg
     3841 aggcggcgcg gctgcgtctc gaggcggcgg ccgcctccgg caggatgccg catgccgtgc
     3901 tggtgcacgg cccgagcggc gtcggcaagg agcggtttgc cggcgccgtg gcggcggcac
     3961 tgctctgcgc gcagcgcggc gatcgcttcg cagcctgcgg agcttgcccc gagtgcgcgc
     4021 tcgtgcgcgc gggctctcat ccggacctgc actggctgcg gccagcggcg gagaagaaat
     4081 cgaccggagt ggaccaggtt cgcgagacgt gcgagcagct cggcatgacg agcatgcgcg
     4141 gcgggttccg cgtcgcgatc gtcgcgccgg cccaggcgat gaccacgagc gcacagaacg
     4201 cgttgctcaa gacgctcgag gaacctgcgg cgcgcacgct gctcgtgctg gtcacgtcgc
     4261 gtccttcggc gctgctgccg accttgcgca gccgctgcca gcggctggag atcgcatgtc
     4321 ctccggagca acaggctgcc ggctggctcg agcgcgaaac cggcgcgccg gtcgcggacg
     4381 gcctgctcga gctcgccggc ggggctccgc tgcgcgcgct ccagatcgca ccgcactacg
     4441 gcgcactcga cgcacagatg acggagttac tggccgggct gctcgccggg cggagcgagg
     4501 cgtgcgccgc agcggcggac atgatgggcg agggcctgcc cgtgcgtctc gactggctgg
     4561 agcgatggct ggggcggctc gtccgtggcc gcgcgggatc gaacgcgacg cgcctcacgg
     4621 ttcgaggggg cgccgtgttg caaagggcag ccgcggaagt gaacataagc gccgctttcc
     4681 gcatcgtgga ccgtgtccgc gaggtccgcc gcctcctcga ggggtccgcg gccccgcagc
     4741 tgctggtcga ggcgctgctc gtcgacgtcg ccgccgcgct gggaagcaga ggagttgcac
     4801 gatggccgtg acgaggcaac cgaacaagcc ggggctgctg acgctcacca tcaaggacaa
     4861 gagcgcgctg tacctcgcct acatgccatt cgtgaagaac ggcggcctgt tcattcccac
     4921 cagcagcaat tatcgcctcg gcgacgaggt gttcatgctg ctgaacctca tgggcgagga
     4981 cgagaagctc ccggtcgccg gccgcgtcat ctggatgacg ccgaagggcg cgcagggcaa
     5041 gcgcacggcg gggatcggcg tccagttcag cgagcaggat cgcggcaata cgcagcgcaa
     5101 gatcgaggcc tacctcgccg gcgcgctcgg cggggacaag ccgacccaca cgatgtagcg
     5161 atcagccgac ggcgttcaac gcgtcggcca ggtccgtcgt ggcgaggccg ccgcgcagca
     5221 ccgaggtgtc gaacccgcgt tcgctcagca ggtaggcggc cgcagagctg cgccgcccgt
     5281 tgtcgcagca gacgacgtag ggcacggacg tgtccaggga cttgagcttc agccggatga
     5341 agtacagcgg cacgttgagg gcattgtcga agtgacagtg ctcgtactcg ctcggcagcc
     5401 tgacgtccag ccactggcca ccgccggcaa cgatgcgccg cgcctcggtg tagtccaccc
     5461 agcgcagcat cggctcattc agcagcgagt tgaagtcttc cttgccgagg cgcatcaggg
     5521 tgccgtcggt gagcatggtg accgttgcgt tgcggggcgc gccggagacg agagcttcct
     5581 cgccgaaggt gtcgcccatg cccagttccg cgagcttgat gccctcgcgg tttagcggcg
     5641 tctcgcgcgt cacggcacag cggccgctga cgatcacgta gaaatagtcg ccctcgtcgc
     5701 cctggcggac gatggtctcg ccggccgtga actcgacgcg ctgcatgcgc atgaacacag
     5761 cctgcagatt cgctggcgga atcctgtgga aggcgcggct ctgcagcagc accgtcatcc
     5821 agtcgccacc ggacccggcc gcgttcaggt cgcccacctc gtaggtgccg gtctggtccc
     5881 acgtgatcag gacgtcgagc aggtcgctgt cgatggcgag gtattcgatg cggtccgtgg
     5941 cgacccgcgc cgtgcaacgg cgggggagga tcggcgcgag cgggtgccgc gcgtcctgcg
     6001 tcccgccctt caccgagccc acgatgcggc cgtcggtgag gtagtccacc gccccggaga
     6061 ccagatagat cgtgcgcttg tcggtgtcgc cttccttgaa cagcacgcgg ccgtgaccca
     6121 gctcgcgcag cgtggtcttg cgggcgagcg cgcgcaggtt ctcgttcttc agaccgtcga
     6181 gcggcgagaa ggaacgcagc agcttggggt cgagggtcga agtctcggtc atcgcgtcgc
     6241 tcggcgtcgc gccgaccagg gattcccggg cgcttcttcc atggcccggc atcgtatcac
     6301 aggcgcccgg tcctccattc gccttcaggc cgccgcgcgc tggcggccct cgacgcggaa
     6361 ttcgcagatc agggggcgat ggtcggagta gcgcacgtcc gccacggaga acgcgtcgat
     6421 cacgatgtcg cgcgtgtgca ggatgaagtc gagttccatg cgcggccgcc gtgccgggta
     6481 ggacggcacg cgatcgcggt tcgcgctctt caagcccgcg gcctccatga acagcgccat
     6541 ctcgtgctcg ccccagaacg tgttgaagtc gccggcgacg atcacgggct tgcgcgaggc
     6601 gcggaccagc tcgaagaggt gcctgagttg cgtctggcgg tgacggaact tgagcgacag
     6661 gtgcacgagg aacacgcaga gctcgtcgag ctcgagctcg atgatgagcc gcttgatgcc
     6721 ggtctcgaag taatggaagc gctcgccctc gacgcgcggc gcggcgagga aggcgttcgc
     6781 ctgcttgttc acgatcggca cgagggtatt gagcgagttc gtgccgtact tgcactggta
     6841 ggtggagtag tggcccaggg actccgcgat cacctcggcc tggttcacgc ggcccgagcg
     6901 gatcgagccc gtatccacct cgaccaggcc gacgatgtcc gggtcgaccg agcggatgaa
     6961 tcgcgtgatg ccctcgagga cgcgcgggtt gctgcggagg taacccgccc cggggaccgg
     7021 aaggtggaag gcggcgcccg tgccggtggc gtagcggatg ttgtagagca gaaagcgcat
     7081 ggacgtcgca atcttatcgg gccgtgcccg gatccgatac ggcgctggta gactaggccg
     7141 cccaggagga ggcaagccgc tcccgcccgg gacggaggta ccgtgagcag cgtatcccag
     7201 caaaatccga tccgcctgtt cgtctgccat gtctgggtcg aggacgacga ctaccagcgc
     7261 gtgttcgagt acctcgagag cgcgcccagg ttctattaca agaacacgag caccccgggc
     7321 gaccgtccgt cgggggatcg tgaagtgctg cgcgaaaacc tgcggcgcca gatcgtcgaa
     7381 gccgagatcg tgatcatgcc cgcgtcactc taccgccggc accacgactg ggcggagttc
     7441 cagctgcatt gcgccaaggc gttcgacaag cccgtggtgg tgctggaacc gttcggctcg
     7501 cacgacacga ttgcgccggc cgtgctggag ctgggtgacg aggtcgtgcc ctgggacttg
     7561 cggcagatcg tggatgcgat caagcgccag gcgcgtcacg aggagacgac gcgttacgac
     7621 gtcatcgagt tcaagctgga ctgacgccgg cgcgactatt cgtcgccgac gagccgcaac
     7681 tgccttgaga gcgcgggcga aggttcggcc tggcgacccg caacgttgac gtagaggtcc
     7741 agcatcgccg atgcgtgctg gctcgcgtcc gcttcggaca tcttccttat cgtcacttcg
     7801 accgcaccga gtccgccggc cggctgcgcg gtgctcagtg ccgactcgag cgcgcgataa
     7861 gagaccgcga gatcgtcggg tagtgcgtcg acttccatgt cacgcaggtg ctgcaggtac
     7921 gccgcgcaca gtcgctgctt gaccggaccc ggtccgacga gcagttcggc tgcgtcgcgc
     7981 aggcgtcgca tgcacgcttc actcgcatcc ggtttctgag aaataaaatg ctttgaaatc
     8041 atttagatat gatgcatttc aaaaacaatc ctaccgcgcg cgggcgcgcg gcaccgtgat
     8101 ccgcccgttt ccggcgaata tacgccgaga tctcgccaat acaagcggtt gcgtcatttt
     8161 tctcggaagc tcggcagccc atgcacgagc ttggggcgat atgagtcact gccggtggtg
     8221 cgcccggaac tccgctggag tcgtcgaatg agccgcgcgg aagcttgcgg tcaggtggct
     8281 ctgcgaactg aagccgcatt cttctgcgac acgcgagatg ggcatcgagg actgggtgag
     8341 gagcttgcgg gcgaggtcga gccttgccgc gaggacgtag cgatgcgggg ggacgcccac
     8401 ggcgtcgcgg aagatgcgcg aaaaatgggc gaggctgacg ccggcgcgca ccgccagggc
     8461 cggagcggac aggtccgcag ccacgttcgc gtggatgtgg ttcagcacgg cctgcagccg
     8521 cgaaaaatgc acgtgccgcg ggttgatgcc gcgcgtaccg ggtgtcacga gcgcgcggta
     8581 agtctgctcg atcatcaccg cggccagctg ttccatgaag acggcgtcgg cgcccggccc
     8641 tttctgcagt tcgcccacca gctgcaacgc cgccgcgccc accagggagt cgccgaagct
     8701 gaccggctct ccgcgtgatc cagcgagcat ggcgaggctc tgcatgctct tgctggcagg
     8761 ctcgaggaag tagaacaccg cgaaatcgac ggttcccgaa tagtgccagt gcgtggcggt
     8821 cgcgcccggg accagcagcg actgggttgg cagattcatg ctgcgccacc ggctgggatc
     8881 accctcccgc acgcgcccgc cgccgaactg gaccacgagg gccgcggcca ggttcatggc
     8941 cgacaccgtt cgctcgatgt cctgatagat atagcgctcc gcgaacagcg gcgtaggccc
     9001 cgggatgcga ccggccacga tcggctgatg ccccaggtcc ttgcgtacct tgaaccagac
     9061 cggtcccgcc atgcgtccgc ctgggttgag cgatttctga taaagatacg cgcattccct
     9121 gaaagccgcc acggagcgcg cgggtacaga atccgcgcac ttatcggcgt gactggggag
     9181 accgcgatga tcaagctgaa gtcgaagctg accctgccgg ccgtcggcct gacaggattc
     9241 gagctgcccc tgaccgaaga agaggcggcg gtgcaggccg gagtgcatcg cttcgccaag
     9301 gacgtgctgc gcccactggg cgccgaactc gacaggatga ccgcggagga ggtgattgcc
     9361 ccaggctcgc cctactggag cgtgttcccc gagttcgcga aactcgggct cgacccggcc
     9421 atgatgagcg agttgcctcc ggaggtcgcg atccgcctgg agtcgctgat cctcgaggaa
     9481 ctgtcgtggg gcgacgctgg cctcggagtc acgctggccg tggccggttt cccgctggag
     9541 atggcggcgt cgctcggcaa caaggaactg gtcgatctgt gcacgggccg gatcggctgc
     9601 tggatgatca cgcacccgga caagggcagc gacgtgcagg tcttcgacat ggcgcgggaa
     9661 tggcccgccg gccagccggg gaacaagggc aacatgtggg gcaaggtgca gggggacgag
     9721 atcgtcatca atggccaatg ctcggcgtgg gtgtctaacg gcgccgtcgc gcagatggca
     9781 ctcggttaca tcggggccga atacgacggg agcttcttcg aaaaggacgg ccgcccgcat
     9841 ggcatgacgg tgttcatccc gctcgacctg ccgggggtct cgaagggcaa gccgctggac
     9901 aagatcggcc agcggtccct gccgcagggc gagatctact tcgacaatgt ccgtatcccg
     9961 aagcgcttcg gggtcgcgct ccgcgacgag tactacggca acgtggcctc ggtctggtcg
    10021 ttcgccggga cagccatggc gcagggattc acgggagtgg cgcgtgccgc cttcgagctc
    10081 gcgctgcagt actgccacga acgcaagcag ggcggcaagc tcctgatcga tcaccagctc
    10141 acgcggtatc ggctcggtga catgctgcgc cgagtggaac tctgccgtgc cgtggcacgg
    10201 cgctcgctgg cgtatgcacg gctgtcgccg cagagccacc cgtacgtcac ggccgcgggc
    10261 aaggtgacgg tgacccagga agcgatgaag gtcgtggacg aggcactgca gctcttcggt
    10321 ggagccggca cctcccgcga gtacccgatc gagaagctat accgggacac gcgcgccgcg
    10381 ctgatcgagg acggcgagaa ctacgtgctg acgatgcgcc ttggtctgct ggcacaacag
    10441 ctttacgcgg agggctgggc acaggcttag caacgtccga tccataaacc acctggccgg
    10501 ggcaggactg tcgccccggc cgcatcgaat caggagagag cacatggcca agtatcccgt
    10561 cgtcgtctat ggagccagcg gctacaccgg catgctgatc atggactggc tgatcgacca
    10621 gaagatcccg ttcacggccg tcgcccgcaa cgccaagagg acccaggaga tgatggcgca
    10681 gcgcgtcgtg cgcctcgagt cggccaccta cgagatcatc gaggccgagc acaaggtcga
    10741 ctcgctgacc aaggccttca agggggccaa ggtcgtgtgc aacacggtgg gcccgttcgt
    10801 gaacttcggc ctgaccggcg tcgaggcggc gctcaaggcc gggtgccatc acatcgacac
    10861 gacgggtgag caggcctaca tgcgcgcggt gcgcgacaag ttcggtgagt cgttccgaca
    10921 ggcgggactg ctcgtctcgc cgtcgattgc ctacatgtac acgctgaccg agatcgcggc
    10981 cgagctcgcg ctcgagacgc cgggcatcga cgcactcgag accggtacgc tggcccgcgg
    11041 cccgcgggct ggctcaggcg tgacggtcgg atcgacagcc tcgatcttcg agatgtaccg
    11101 aagcgaggcc tgcttcctct gggagaagga gttggtgccg catgcacctg gcacgtccgc
    11161 aagcatcgtc gacccgaatt tcgcgcaacc cgtcttctcg ctgccgtggg gcggaacttc
    11221 gctgccggtc tatttcgagc acgactcgcg cgtgcgcagc tgcatctcat acgtcggttt
    11281 ctacgacaac aacgtcatgc agcaggttca cgcgctcggc cagaagtggg aggcggagta
    11341 caagaacctg cccaaagagc accaggacgc ggtcctgaag cagatcgtcg actcgaccac
    11401 accggcgatg ccgccgcggg agcgcacgac gctgcagcgt tcggtcgact tcgcgatcgg
    11461 gcgcgggcag ctggctgccg tccgggcgac cgtgcacggc gtgaccccgt acatctcgac
    11521 gggagccgta cagacggcgg ccgtccttaa gctgctcgat ggcgaaacga acagggtcgg
    11581 attcgcgtca ggatccaaag cgttcgggca ccgctacctg ctcggcttcc tcgagcagcg
    11641 cggcctcgcg tccgcctccg tcaccaagct gtagatcgag aatcggcaat aacggcgtgc
    11701 cgcgcccggt cgggcgcggc gccccattca gggggcgaca tggcgatcat cgacttcttc
    11761 gaccgcggct ggcgcatcaa tccgaagggt gcggcctaca tccaggacga ccggagctat
    11821 tccttcgacg acgttggcga gctgtcctgc cgcatcgcca acggcctgct cacactcggc
    11881 ttgccgaagg aaaccaaggg cgccgtctgg gccaccaacg acgtcacggc gtggacctgc
    11941 acgctgggac tctggcgagc caacatgtgc tggattccgg tgggtgcgcg caatccggcc
    12001 gaagagaacc actacgtcct cgatgcgttc gactgcgagg tactgttttt ccagaagtac
    12061 ttcgcgccgg tgatcgccga actgcagccg cggctgccga agatccggct gtggatctgc
    12121 accgacggcg acgcgccgga ggtcaagggt gccacgtcgc tccagtcctg ggtggccggg
    12181 ctgccgccca cgtgcccgcg cgtggcggtg gacatgaatg acgtcgtgat gctgtcctcc
    12241 acgggcggca ccacgggcaa gcccaagggc gtgatgaaca cgcaccgcag cgcccagact
    12301 ttctgtgcgc atttcatgat cgactgcccc tacgaggcgg acgaggcgcc cgtgaatctc
    12361 gccgcggcgc caatgaccca cacggcgggc ctgctctcca ttccatgcac ggcacgtggc
    12421 ggaacggtcg tcgtcgtgac caagccagac cccacgatcc tgctcggggc gattccgaag
    12481 taccgcgtca cggagctgtt cctgccgccg acggtgatct accggctgct cgacatcccg
    12541 gacctcggca agaaggtgga cttctcgtcg ctcaagtact tcatgtacgg agccgcgccg
    12601 atgtcggtcg aaaagctcaa gcaggcgatc caggtcatcg gcccggtgat ggcgggcggt
    12661 tacgggcaga ccgaggcgcc ggcgtcgatc tcctacctgc cgccgagcgc gcacttcgtg
    12721 gacggcaagc tcgcctccga cgagcggctg tcgtcggtcg gctacccgaa cccgctgata
    12781 cgcgtcgaga tcatgaatga cgccaacgag atcgtgccga agggcgagac gggcgagatc
    12841 tgcgtgcgcg gcgacctggt gatgaaaggc tattacaagg atcctgtgaa gaccggcgag
    12901 acggtgatcg acggctggct ccacaccggc gacatcggcc acctcgatgg cgagggcttc
    12961 ctgcacatca ccgaccgcaa gaaggacatg atcatcaccg gcgggttcaa cgtg
//
LOCUS       DAOMFD010000048        12844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1202_length_12844_cov_32.498632, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000048 DAOMFD010000000
VERSION     DAOMFD010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1202_length_12844_cov_32.498632"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2502
                     /locus_tag="PL152_04500"
     CDS             <1..2502
                     /locus_tag="PL152_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006812181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR48572.1"
                     /translation="QNQQVAIGELGGTPSIPGQQLNAPITAQGRLQTPEQFRAIVLRG
                     NPDGSTLMLSDVARVELGVETYSYISRFNTKPATGIAVSLATGANALQTAKALEAKLQ
                     QMEQTFPPGLKTVVPFDTTPFVRVAIQGVVTTLFEAVALVFLVMYLFLQNLRATIIPT
                     IAVPVVLLGTFAVLSALGYSVNMLTMFAVVLAIGLLVDDAIVVVENVERIMHEEGLSP
                     LEATRKSMDQITGALVGVGLVLAAVFVPMAFLVGSTGIIYRQFSATIVSAMALSVAVA
                     IVLTPALCATLLKPAAETDRTSGFFGWFNRTFDRGSRHYQGVVRGMLSRRGRYLAVFA
                     AMAGLAALLFVRLPSAFLPQEDQGWMMAQIMTPPGATQQRTMNSIRAVEKYFLENEKD
                     AIRSIFTVQGFSFSGSGQNAGIAWINLKDWSERTSKELGVDAVAGRAMGALFQIKDAF
                     AFAFAPPPLPELGTSKGFVFNLKDDAGLGHDALTAARNQFLGMAMQDKLLANVRPNGQ
                     EDAPQFRIDVDNKKAAALGVSIDDISETLAAAWGGQYIDDFVDRGRVKRVYVQADAPF
                     RMVPEDFARWSVRNSKGEMVPFPAFASWRWDFGPPRLERYNGSPSFEINGEPAPGVSS
                     GTAMAEVEKLVAQLPRGVGLEWTGQSYQEREAGAQAPLLYTLSILVVFLCLAALYESW
                     AIPTAVLLVVPIGILGTVLATTLRGMERDVFFQVAMLTTVGLSSKNAILIIAFAKDNL
                     ERGMDLIEATMTAVRIRLRPILMTSLAFGLGVLPLAIATGAGSGAQRAIGTGVLGGMI
                     AGTLLGVLFTPLFFVVVERLLGKGGDPAPTPMPHD"
     gene            2591..3478
                     /locus_tag="PL152_04505"
     CDS             2591..3478
                     /locus_tag="PL152_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012204307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HQR48573.1"
                     /translation="MVPNHPEQPARVVVIAEELRARGALDYLSQYEAPRATDEQLARV
                     HPAEYLQYIRAKAPAAGLEPLDPDTTMCPDTLEAALRAAGANVLAVDLVMSGRARRAF
                     CNVRPPGHHAERASAMGFCFFNNVAVGAAHALAHHGLQRVAILDFDVHLGNGTEDIFR
                     DDPRVLLCSMFQYPLYPDMNPASVPGHIVNCPLPPGSGSDEFRTAVTQHWLPALEAFA
                     PEMLFLSAGFDAHASDPLAELRLATDDYAWVTDVACAVADRHAQGRVVSTLEGGYDLP
                     ALAMSGAAHVERLMATEAA"
     gene            complement(3455..4567)
                     /locus_tag="PL152_04510"
     CDS             complement(3455..4567)
                     /locus_tag="PL152_04510"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator
                     ligand-binding domain-containing protein"
                     /protein_id="HQR48574.1"
                     /translation="MLLMFYDSLPGVPGSHRARSRTARSITVKPQAQRFPARYFVQFL
                     DCVESLGVERDAVLRAARMRPLEDPRAQVNLRQVEALFAAAERLSGRLDLGFEFGRRV
                     KVTSHDILGYALLTSPTLRGALRLLANYQRLINPAFTLELQRRTSHVDLVYRPAMPLS
                     HRAMRVLEEAIAVSNLHEFRGLLQRDLPATDVHFSVERPAHAERYRDLGLVRMHWGEM
                     LPGLRMSLDAALLDLPLALADPRAMQAAEERCAAMMRSARGGRRWSEWCRMMLREAED
                     CQPSLEQLARFVNLSARTLGRYLEAEDAGFRELSLQVRTERAQQLLAVGKHSVTEVAY
                     RLGYSDVASFIRSFRDRTGRTPGAYAAWRSSRLGRH"
     gene            4656..6362
                     /locus_tag="PL152_04515"
     CDS             4656..6362
                     /locus_tag="PL152_04515"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HQR48575.1"
                     /translation="MTDVTGGELLARALANEGIRTIFGLPCPEIDPLLAALEANGIRR
                     IPVRHESAGAHMAEGLYKTTGQVAAVLGNPGPGSANLIPGVVTARHEGVPMIAITSQH
                     RAGIVYPSTPATFQGQDQLDLFRPAVKWGGPIFEWTRIPELVRMACREMWAGRPGPVH
                     LEVPGPIMYATGDPANAPIYGPGSGRAGAPQPSDLQLDEAARMLADAAAPVIFAGTGV
                     DRAGANAALVELAELMRCPVIPSMAGRSVIAHDHPLCFVSQSPAADALRKEADVMLVV
                     GSRVGNLDVPFDKYWGDTGHCRIVHVDVDPRNIGVSRPIALGIVADARATLTRLATRL
                     KDAKVASRGRKDLDRLRAEHAAWVQGLQAVVAGWSGPGVHPGQACAVVGAVFGRDAIY
                     CADGGNTSLWASLALPSTRPSSYHGILELGMLGVGIPVAIGAKLGAPSREVVCVTGDG
                     AAGFNVMELQTAVQQGIKVTVVVMAEGEWTMEIPNEVKNWGRDFGTSMSEVRWDVVAK
                     GLGCHGEYVDSIADLAPALSRARAHDGPALVCVRTSKEANLAVPEAVVGRFFEVYFGP
                     DA"
     gene            6359..7186
                     /locus_tag="PL152_04520"
     CDS             6359..7186
                     /locus_tag="PL152_04520"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR48576.1"
                     /translation="MKPLKDRRVLITGAASGIGYECARSFAKRGARLAISDVDAAGLD
                     KARAELVASGASCFAMTCDVANEDSVNAFAAAVRRDVGPVDVLVNNAGVAYLGGFEET
                     PLAQWRRILDINVMGIVHCVRAFLPAMRAAGGARKIVNVASLAGFAPAPNMSAYAASK
                     HAVVGLSEALALELHDTDVSVLIVCPGIINTAIVHVSPTSASISDAQKEKLQRYYATV
                     GCHPSVVAEDIVRAVERDAIVLPTGPMAGVGYRLMRLSRRLARRLTISGAKKSGYLP"
     gene            7339..7725
                     /locus_tag="PL152_04525"
     CDS             7339..7725
                     /locus_tag="PL152_04525"
                     /inference="COORDINATES: protein motif:HMM:NF013304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HQR48577.1"
                     /translation="MNVELTMLTWTVSLWVAQLLVAVAGASTQFPMNVLAGNRETTVE
                     GRGWVGRAQRACRNLLESLPPFAALVLVAHAAGVSNATTVMGAQLFFYARVAYAIVYI
                     AGIPWLRTAVWTAGLVGLLMILSQLV"
     gene            7828..8535
                     /locus_tag="PL152_04530"
     CDS             7828..8535
                     /locus_tag="PL152_04530"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR48578.1"
                     /translation="MAPGQQVLDIGSGDGELAVAAAAIVGPSGRVLATDVTLERLAGL
                     AHRLPPGPPRSPIEIQACAAEDLALLPGSFDVALARNCIMYFRDLDRALRNVRAALRP
                     GARCIASVYGPLEREPFHAIPIAAVQRRCHLAEPLPEYARAFRLGATDLRAALRRAGF
                     RDVHLRTVAVTRTYPSISALLEQLRTSRSLGDLLSCLPEGAVDQAWREIDAGFRAFAG
                     PAAVRIPGEQVVVTGTT"
     gene            complement(8572..9015)
                     /locus_tag="PL152_04535"
     CDS             complement(8572..9015)
                     /locus_tag="PL152_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48579.1"
                     /translation="MSRATIAAVAVSMATACATRAVVPDAPSVSAEVPVTGARSHAPG
                     EYPDAMALAARGADPLAIALGVAGPFQGTRQEIVQVNEGGELPSKSIVTLVRDGLLDD
                     SLRGERWKVTLGRSQSGTWAITQVQRSVRCRRGGDPDRYVAGPCP"
     gene            9132..10598
                     /locus_tag="PL152_04540"
     CDS             9132..10598
                     /locus_tag="PL152_04540"
                     /inference="COORDINATES: protein motif:HMM:NF024860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR48580.1"
                     /translation="MTNELAAASSAYTERRYGDALASLEQAAAVKSADPLHWQRVLEI
                     ATRLGDDDVALVAARRLLEKTPQDPAQRARVADLLSETGAVRDALGISQKLLDEHPQH
                     PMLPLVIGIQLGRLGRVEEALRSLRIAIRRSPQSALAWENLANLRTFQPGDPDIVELE
                     RLARVSGERPEAAGFAYAYAKACDDVGDFDRAYEWFARGSARVLGNRVPRMDALFKEA
                     EDVLAAFPAERLTAGPLSDRPERPILVIGCARSGTTLIERILSTAPGAKSGGELKLLQ
                     LACLDFTPPSPERVDSYVRSAGGEAAAWRRVAETYVRKLRARFGQADGMIDKGLVNYL
                     YVGALALGLPQARIIHMRRNPMDVAWSCFRRRFHAGLEWSYQFDSIAAFLSVYERVSA
                     HWERTLPGRVLAVDFERLVSSAESETARIFEFAGLQRPDDWRSFHEKRGVVLTASQLQ
                     VRRPLNADGVGAWRRYEKHLGPLQDALRRHGLEGASGA"
     gene            complement(10645..11088)
                     /locus_tag="PL152_04545"
     CDS             complement(10645..11088)
                     /locus_tag="PL152_04545"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HQR48581.1"
                     /translation="MSGHRRLDVERFVAARRKQILAYSRFKVTGDRADLVAMTSTVSC
                     RSCRACCIEYADTPLLPVDSAEGLDVEIRDGKRFLRKKANGECVHLGEQGCTVYERRP
                     STCRVFDCRDFAVSKLSNPDLPLLNAAIAQWSLSGKLPKAPTGGS"
     gene            11345..11668
                     /locus_tag="PL152_04550"
     CDS             11345..11668
                     /locus_tag="PL152_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013721969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR48582.1"
                     /translation="MKEPAVLRALAALSQASRLRVFRVLVVAGQAGATPTAIARRLKV
                     APATLSFHLKELANAGLVSQQRSGRNLIYRADYGSMNDLLAYMTENCCQGEDCGSGPA
                     RACKS"
     gene            11679..12173
                     /locus_tag="PL152_04555"
     CDS             11679..12173
                     /locus_tag="PL152_04555"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_250969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HQR48583.1"
                     /translation="MTTNVLILCTHNSARSVLAEAMLNEYASRYGRDVRAFSAGSAPR
                     GRVNPLALAVLADAGVSTQDLHSKGWEEFARPDSPRMRAVITVCDSAAGETCPIWPGG
                     PVQVHWGYPDPSEAPGDEVARRTAFDLTRQAIGYRMLQLLRLPFESLDDAELRDALLE
                     IARS"
     gene            12183..>12844
                     /locus_tag="PL152_04560"
     CDS             12183..>12844
                     /locus_tag="PL152_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenical-resistance protein"
                     /protein_id="HQR48584.1"
                     /translation="MTPVASRLSFLDRYLTAWIFAAMALGIGIGWFVPDVETVMNRFT
                     VGTTNVPIALGLILMMYPPFAKVRYEDLPDVFRDRKVLALSLVQNWLVGPVLMFLLAI
                     TFLADKPEYMAGLILIGLARCIAMVIVWNELAGGSTEYAAGLVAFNSIFQVLFYSLYA
                     WVFVTVLPPMFGLHGSVVDISIAQIAKSVFIYLGIPCIAGLLTRVVMLRFVSKDWYHQ
                     HFV"
BASE COUNT         1971 a         4469 c         4352 g         2052 t
ORIGIN      
        1 cagaaccagc aggtcgcgat cggcgagctg ggcgggacgc cgtcgatccc gggacagcag
       61 ctcaacgcac cgatcaccgc gcagggtcgc ctgcagacgc ccgagcagtt ccgcgcgatc
      121 gtcctgcgcg gcaatcccga cggctcgacg ctgatgctga gtgacgtcgc ccgggtcgag
      181 ctcggcgtcg agacctacag ctacatcagc cggttcaaca ccaagccggc gacgggcatc
      241 gcggtgtcgc tcgccaccgg cgccaacgcg ctgcagacgg cgaaggcact cgaggcaaag
      301 ctccagcaga tggaacagac gttcccgccc ggcctgaaga cggtggtgcc gttcgatacc
      361 acgccattcg tgcgggtcgc gatccagggc gtggtcacca cgctgttcga ggccgtggcg
      421 ctcgtgttcc tggtcatgta cctgttcctg cagaacctgc gcgcgacgat catcccgacg
      481 atcgccgtcc cggtcgtgct gctcgggact ttcgccgtgc tctcggcgct cggctactcc
      541 gtcaacatgc tgaccatgtt cgccgtggtg ctcgccatcg gactgctggt ggacgacgcc
      601 atcgtggtcg tggagaacgt cgagcgcatc atgcacgagg aaggcctgtc gccgctcgaa
      661 gccacgcgca agtccatgga ccagatcacc ggcgcgctcg tcggcgtggg gctggtgctc
      721 gcggccgtgt tcgtgccgat ggcgttcctg gtcggctcca ccggcatcat ctaccggcag
      781 ttttccgcca cgatcgtctc ggcgatggcg ctgtccgtcg cggtcgcgat cgtgctgaca
      841 ccggccctgt gcgccacgct gctcaaaccg gcggccgaga ccgaccgcac cagcggcttc
      901 ttcggctggt tcaaccgcac cttcgaccgc ggcagccggc attaccaggg cgtggtgcgc
      961 ggcatgctct cgcggcgtgg ccgctacctc gcggtgttcg ccgccatggc cggactcgcc
     1021 gcattgctgt tcgtgcgcct gccgagcgcc ttcctcccgc aggaagacca gggctggatg
     1081 atggcccaga tcatgacgcc accgggcgcc acgcagcagc gcacgatgaa ttcgatccgc
     1141 gccgtcgaga agtacttcct cgagaacgag aaggacgcga tccggtcgat cttcaccgtg
     1201 cagggcttca gcttctccgg cagcggccag aacgccggca tcgcctggat caacctcaag
     1261 gactggtccg agcgcacgtc gaaggaactc ggcgtcgacg cggtcgccgg gcgtgccatg
     1321 ggtgccctgt tccagatcaa ggacgcgttc gccttcgcat tcgcgcctcc tccgctgccg
     1381 gaactcggca cgtcgaaggg cttcgttttc aacctgaagg acgacgcggg cctcgggcac
     1441 gacgcgctca ccgccgcgcg caaccagttt ctcggcatgg cgatgcagga caagctgctc
     1501 gccaacgtgc gcccgaacgg gcaggaggac gccccgcagt tccgcatcga cgtcgacaac
     1561 aagaaggccg ccgccctcgg cgtgtcgatc gacgacatca gcgagacgct cgctgcggcg
     1621 tggggcggcc agtacatcga cgatttcgtg gatcgtggtc gcgtcaagcg cgtgtacgtg
     1681 caggcggatg ccccgttccg catggtgccg gaggatttcg cgcgctggtc cgtgcgcaat
     1741 tcgaaaggcg agatggtccc gttcccggca ttcgcgagct ggcgctggga ctttggcccg
     1801 ccgcggctgg aacgctacaa cggctcgcct tcgttcgaga tcaacggcga gccggcgccc
     1861 ggcgtcagct ccggcacggc gatggccgaa gtcgagaagc tggtggcgca actcccccgc
     1921 ggcgtcggcc tcgagtggac cggccagtcg taccaggaac gcgaagccgg cgcccaggcg
     1981 cccctgctct acacgctgtc gatcctcgtg gtgttcctgt gcctcgcggc cttgtacgag
     2041 agctgggcga tcccgacggc cgtgctgctc gtcgtcccga tcggcatcct cggaacggtg
     2101 ctggcgacga cgctgcgcgg catggagcgc gacgtattct tccaggtggc gatgctcacc
     2161 acggtcggcc tgtcgagcaa gaacgcgatc ctcatcatcg ccttcgccaa ggacaatctc
     2221 gagagaggca tggacctgat cgaggccacg atgaccgcgg tacgcatccg cctgcggccg
     2281 atcctgatga cctcgctcgc cttcgggctc ggtgtactgc cgctcgcgat cgcgaccggc
     2341 gccggttccg gtgcgcagcg cgccatcggt acgggcgtgc tcggcggcat gatcgccggt
     2401 acgctgctcg gcgtgctgtt cacgccgctg ttcttcgtcg tggtggagcg cctgcttggc
     2461 aagggcgggg atccagcgcc gacgccgatg ccgcatgact gagtcatcgt cagcggccgg
     2521 ccgagtggca aagggcggag accaacgtct cgtcgcattc atctcgcacc cggcgtgccg
     2581 gctccaccag atggtgccga atcaccccga gcagccggcg cgcgtcgtcg tgatcgcgga
     2641 ggaactccgc gcgcgcggcg cgctcgacta cctgtcgcag tacgaggcgc cgcgggcgac
     2701 ggacgagcaa ctggcgcgcg tgcatcccgc cgagtacctg caatacatcc gggccaaggc
     2761 acccgccgct ggcctcgagc cgctcgatcc ggacacgacg atgtgcccgg acacgctcga
     2821 ggctgcgctc cgggccgccg gcgcgaacgt gctggcggtg gacctggtca tgtctggtcg
     2881 ggctcgccgg gctttctgca acgtgcggcc gcccggtcat cacgcggagc gagcgagcgc
     2941 gatgggattc tgtttcttca acaacgtcgc cgtcggagcc gcgcatgcgc ttgctcatca
     3001 cggcctgcag cgcgtcgcga tcctcgattt cgacgtgcac ctcggcaacg gcaccgagga
     3061 catcttccgc gacgacccgc gcgtgctgct ctgctcaatg ttccagtacc cactgtaccc
     3121 cgacatgaac ccggccagcg ttcccgggca catcgtcaat tgtcccctgc ctcccggcag
     3181 cggcagcgac gagttccgca cggcggtcac gcagcactgg ctgccggcac tcgaggcctt
     3241 tgctcccgag atgctgttcc tgtctgctgg cttcgacgcc cacgcgtccg acccgctggc
     3301 cgaactgcga ctggccacgg acgactacgc atgggtcacc gatgtcgcat gcgccgtcgc
     3361 ggaccgccac gcgcagggtc gcgtggtctc cacgctcgag ggcggttacg acctgccggc
     3421 actggcaatg agcggcgcag cgcacgtcga gcggctaatg gcgaccgagg cggcttgacc
     3481 gccacgcggc gtacgcaccg ggcgtgcggc cggtccggtc gcgaaagctg cggatgaaac
     3541 tcgccacgtc gctgtagccg aggcgatacg cgacctcggt cacggagtgc ttgcccacag
     3601 ccagcaactg ctgcgcgcgc tcggtcctta cctgcagcga cagttcccgg aacccggcgt
     3661 cctccgcctc caggtaccgc cccagcgtgc gtgccgagag attcacgaag cgagccagtt
     3721 gctccaggct cggctggcag tcctccgctt cccgcagcat catccggcac cactcgctcc
     3781 accgtcggcc gccgcgcgcg ctccgcatca tggcggcgca gcgctcctcc gccgcctgca
     3841 tcgcacgcgg atccgccagt gcgagcggaa ggtcgagcag cgccgcgtcg agcgacatcc
     3901 tgaggcccgg cagcatctcg ccccagtgca tgcggaccag ccccaggtcg cgatagcgct
     3961 ctgcgtgcgc cggtcgttcg acggagaagt gcacgtcggt cgcgggcaag tcccgttgca
     4021 gcaggccacg gaactcgtgg agattcgaca ccgcgatcgc ctcctcgagg acgcgcatcg
     4081 cccgatgcga cagtggcatc gccggccggt acacgaggtc cacgtggctc gtgcggcgct
     4141 gcagctcgag ggtgaacgcg ggattgatca ggcgttggta gttcgcgagc aagcgcagcg
     4201 cgccgcgcag cgtgggactc gtgagcagcg cgtagccgag gatgtcgtgc gaggtgacct
     4261 tgacgcgccg cccgaactcg aagccgaggt cgagccggcc gctcaggcgt tccgccgcgg
     4321 cgaacagcgc ctcgacctgg cgcaggttga cctgggccct cgggtcctcg agcgggcgca
     4381 tgcgcgccgc acgcagcacc gcgtcgcgct cgacgcccag ggactcgacg cagtcgagga
     4441 actggacgaa gtagcgggcc gggaatcgct gtgcctgggg cttcacggtg atcgacctcg
     4501 cggtccgcga ccgggcccgg tgcgagccgg gcacgcccgg aaggctgtcg taaaacatca
     4561 gcagcatggc agaaaatatc agtacagtcg cgggtcccgt gtcgagaatc gccgtcatcc
     4621 ggccgcggcc gcaccgcgca cgacgagggg gaaccatgac agacgtcacc ggcggcgaac
     4681 tcctggcacg agcgctcgcc aacgaaggca tccgcaccat cttcggcctg ccctgcccgg
     4741 agatcgatcc actgctcgcc gcgctcgagg ccaatggcat ccggcgcatt ccggtccgcc
     4801 acgaatccgc cggcgcgcac atggccgagg gcctgtacaa gacgaccgga caggtcgccg
     4861 ccgtcctcgg gaacccgggg cccggatccg cgaacctcat tcccggcgtc gtcaccgccc
     4921 gccacgaagg cgtcccgatg atcgcgatca cgtcgcagca ccgcgcgggc atcgtctatc
     4981 cgtcgacccc ggccaccttc cagggccagg accagctcga cctgttccgg ccggccgtga
     5041 agtggggcgg cccgatcttc gagtggaccc gcatcccgga gctcgtgcgc atggcgtgcc
     5101 gggagatgtg ggccgggcgc ccgggcccgg tgcacctcga ggtgccgggc ccgatcatgt
     5161 acgcgacggg tgacccagcc aacgcgccca tctacggccc gggatccgga cgtgcgggcg
     5221 cgccgcagcc gtccgacctg cagctcgacg aggccgcacg catgctggcg gatgcggcgg
     5281 cacccgtgat tttcgccggg accggcgtgg atcgcgccgg agcgaacgcc gccctcgtcg
     5341 agctcgcgga gctgatgcgg tgcccggtga ttccgtcaat ggccggacgc agcgtgatcg
     5401 cgcacgatca tccgctctgc ttcgtatcgc agagcccggc ggcggatgcg ctgcgcaagg
     5461 aagccgacgt gatgctggtc gtgggttcgc gcgtcggcaa cctcgacgtg cctttcgaca
     5521 agtactgggg ggacacgggg cactgccgca tcgtccacgt ggacgtggat ccgcggaaca
     5581 tcggtgtctc gcggccgatc gcgctcggca tcgtcgcgga cgccagggcc accctcaccc
     5641 ggctcgcgac acgcctgaag gacgcgaagg tcgcgagtcg cggccgcaaa gacctcgacc
     5701 gactccgggc cgagcacgcc gcgtgggtcc agggactcca ggccgtggtt gcgggctgga
     5761 gcggaccggg cgtccatccg ggacaagcct gcgcggtggt cggcgcggta ttcgggcgcg
     5821 acgcgatcta ttgcgcagac ggcggcaata cgtcgctgtg ggcgtccctc gccctgccct
     5881 ccacccgtcc gtcgtcgtac cacggcatcc tggagctcgg catgctcggg gtgggcatcc
     5941 cggtcgccat cggcgcgaag ctcggcgcac cctcgcgtga ggtggtttgc gtgaccggcg
     6001 acggcgccgc cgggttcaac gtcatggagc tgcagacggc ggtgcagcag ggaatcaagg
     6061 tcacggtcgt cgtcatggcc gaaggcgaat ggacgatgga gatccccaac gaggtcaaga
     6121 actggggaag ggacttcggc acctcgatga gcgaagtccg ctgggacgtg gtggcgaagg
     6181 gactcggctg ccacggggaa tacgtcgact cgatcgccga ccttgccccg gctctctctc
     6241 gcgccagggc gcacgacggc cccgcgctcg tctgcgtgcg caccagcaag gaggcgaacc
     6301 tcgcggtgcc cgaagccgtc gtcggccgct tcttcgaggt ctacttcggg cccgacgcat
     6361 gaagccgctg aaggacaggc gagtgctcat cacgggagcc gcgtcaggca tcggctacga
     6421 gtgcgcacgc tcgttcgcaa agcgtggcgc gaggctcgcc atttcggacg tcgacgcggc
     6481 agggctcgat aaggcgcgcg ccgaactcgt cgcgagcggc gcgtcgtgct tcgcgatgac
     6541 ctgcgacgtg gcgaacgagg attccgtcaa cgcattcgcc gcggcggtcc ggcgggatgt
     6601 cggtcccgtc gacgtgctcg tgaacaatgc gggtgtcgcc tacctgggcg gcttcgagga
     6661 gacgccgctc gcacagtggc ggcggatcct cgacatcaac gtcatgggca tcgtgcactg
     6721 cgtgcgcgca ttcctgccgg cgatgcgcgc cgccggcggc gcgcgcaaga tcgtcaacgt
     6781 cgcgtcgctg gcgggcttcg cgccggcacc caacatgtcg gcctatgcgg cgtccaagca
     6841 cgccgtggtc gggctgtcgg aagcgctggc gctcgagctg cacgacacgg acgtctccgt
     6901 cctgatcgtg tgtcccggca tcatcaacac cgcgatcgtg cacgtgagcc cgacctcggc
     6961 cagcatttcc gacgcacaga aggagaagct gcagcgctac tacgccacgg tcggctgtca
     7021 tccgagcgtg gttgccgagg acatcgtgag agccgtggag cgtgacgcca tcgtgctgcc
     7081 gaccggcccg atggccggcg tcggctaccg gctgatgcgg ctgtcgcgac gcctcgcgcg
     7141 ccgattgacc atctcaggcg cgaagaagag cggctacctg ccgtagcacc gatccagcgg
     7201 ccgtcggtcg gtgcgtgcac gggcctcaga gtcttctggc cacatcggtc gcagcgctcc
     7261 caccgacgag tgtagagttc cccgctggcg gtacgggccg cgctcatcct gacattcaac
     7321 aagcaagggg gatcacgcat gaacgtcgaa ctcacgatgc tcacctggac ggtctcgctc
     7381 tgggtggcgc aactgctggt ggcggtcgcc ggcgcatcca cgcaattccc gatgaacgtg
     7441 ctggccggca atcgcgagac gaccgtcgag ggccgcggct gggtcggacg ggcacagcgc
     7501 gcgtgccgca acctgctcga gagcctgccg ccattcgcgg cgctcgtgct cgtggcgcac
     7561 gccgccggcg tgagcaacgc gacgaccgtc atgggcgccc agctgttctt ctacgcccgc
     7621 gtcgcgtacg cgatcgtgta catcgccggg atcccctggc tgcgcacggc ggtgtggacc
     7681 gcggggttgg tcggcctgct gatgatcctg tcgcaactcg tctgaagcgg cgacgcccgg
     7741 gtcaccgcgg accgtgggac gcagccgccg gcgcagtaca cggaagcgct cgccccagtc
     7801 acggagtgca tgcttgccgg agccgccttg gcgcccggcc agcaggtgct cgacatcggc
     7861 agcggcgacg gcgagctggc agtcgcggcg gctgcgatcg tgggcccgtc cgggcgcgtg
     7921 cttgcgacgg acgtcactct ggagcgtttg gccgggctgg cgcacaggct gccgcccggg
     7981 ccgccgcggt ccccgatcga aatccaggca tgcgccgccg aggacctggc gcttctgccg
     8041 ggcagcttcg acgtggcgct cgcgcgcaac tgcatcatgt acttccggga cctcgatcgt
     8101 gcgctgcgca acgtgcgtgc cgcgctgcgg ccgggcgcgc gatgcattgc gtcggtgtac
     8161 gggccgctcg agcgtgagcc gttccacgcg ataccgatcg ccgccgtcca gcgcagatgc
     8221 catctggctg aaccgctgcc ggaatacgcc cgggccttca ggctcggcgc gacggacctg
     8281 agggcggcgt tacgccgtgc cggattccgc gatgtgcact tgcggactgt cgccgtcacc
     8341 cggacctatc cgtccatttc cgccctgctc gaacaactgc ggacgtcgag gtcattgggt
     8401 gatctgctgt cctgcctccc ggaaggcgct gtcgaccagg cgtggcggga aatcgacgca
     8461 ggcttccggg catttgcggg cccggccgcc gtccggatcc cgggcgagca ggtcgtcgtg
     8521 accggcacca cgtgagacga gaccgtgccg gcacgagagc agcctgcaac ctcaaggaca
     8581 ggggccggca acgtaccgat cgggatcgcc accgcgtcga catcgcacgg accgctgcac
     8641 ctgcgtgatc gcccaggtgc cggactgcga tcgtcccagc gtgactttcc accgttctcc
     8701 acgcagcgaa tcgtcgagca gtccgtccct gaccaacgtg acgatcgact tcgaaggcag
     8761 ttcgccaccc tcgttcacct ggacgatctc ctgtcgcgtt ccctggaacg ggccggccac
     8821 gccgagagcg atggcgagcg gatcggcgcc ccgagctgcg agcgccatcg cgtccgggta
     8881 ttcaccgggc gcgtgtgacc tggcccccgt gacgggaacc tccgcggaga cgctcggcgc
     8941 gtccgggacc acggcgcggg tcgcgcacgc ggtcgccatg gaaaccgcga cggctgcaat
     9001 ggtggcacgc gacatcatcg agtgattgta gcggcggcca gtcgctgaag cgcgtgcggc
     9061 cgcggtccgg ccggatgagc tgcggtcacc ttcggcgaca ggcggccgag gttgcagata
     9121 gactgcgcgc gatgaccaac gaactcgccg cggcatcgtc cgcatacacc gagcggcgct
     9181 acggcgacgc gctggcgtcg ctggagcagg cggccgccgt caagtctgcc gacccgctgc
     9241 actggcagcg agtgctcgag atcgcaaccc gcctcggcga tgacgacgtc gccctggtgg
     9301 ccgcgcggcg actgctcgag aagacgccac aggatcccgc tcagcgcgca agagtcgcgg
     9361 acctcctgtc ggagaccggc gcagttcgcg atgcgctggg catctcgcag aaactcctgg
     9421 acgagcatcc tcagcatccg atgctgccgc tggtcatcgg gatccagctc ggccgtctcg
     9481 gccgggtgga agaggcgctg cgttcgctgc gcatcgcgat ccggcgttca ccgcagtcgg
     9541 cgctggcctg ggagaacctc gcaaacctca ggaccttcca gcccggggac ccggacatcg
     9601 tggagctgga gaggctggcg cgcgtttccg gcgagcggcc ggaagccgcc ggatttgcct
     9661 acgcatacgc gaaggcctgc gacgacgtcg gtgacttcga tcgcgcctac gagtggttcg
     9721 ctcgcggcag tgcgcgtgtc ctcggcaacc gtgtcccgcg catggacgcg ctcttcaagg
     9781 aagccgagga cgtgttggcg gcatttcccg ccgagcggct gacggcggga ccgctgagcg
     9841 accggccgga gcgaccgatc ctggtgatcg gttgcgctcg ctccggcacg acgctgatcg
     9901 agcgcatcct gtcgaccgcc cccggcgcga agtcgggcgg cgaactcaag ttgctgcagc
     9961 tcgcatgcct ggatttcacg ccgccatcgc cggagcgggt cgacagctac gtgcggtcgg
    10021 ccgggggcga ggccgcggcc tggcgccgcg tggccgaaac ttacgtgcgc aagctgcggg
    10081 ctcgctttgg ccaggccgac ggcatgatcg acaagggcct cgtcaattac ctgtatgtcg
    10141 gggcgctggc tctcggcctg ccgcaggcgc ggatcatcca catgcgccgc aacccgatgg
    10201 acgttgcgtg gtcatgcttt cgccgccgct tccacgcagg gctcgagtgg agctatcagt
    10261 tcgactccat cgcggccttc ctgagcgtct acgagcgcgt gtccgcgcac tgggagcgca
    10321 ccctgcccgg acgggtgctc gcggtggact tcgagcggct cgtgtccagc gccgagagcg
    10381 agacggcgcg catcttcgag ttcgccggcc tgcagcgtcc ggacgactgg cgctcgttcc
    10441 acgagaaacg cggcgtcgtg ctcacggcga gccagctgca ggtccgacgc ccgctcaacg
    10501 ccgacggggt cggcgcgtgg cgtcgctatg agaaacacct gggtccgctg caggacgcgc
    10561 tgcgcaggca cggcctggaa ggcgcatccg gcgcctagcg tcggtcgtca ccccgccgcg
    10621 accatccccg cacgccgctc gtgatcaact gccgccggtc ggtgccttcg gcaacttgcc
    10681 cgagagcgac cactgcgcga tggcggcatt caacagcggg agatccggat tgctgagctt
    10741 cgacaccgcg aagtcccggc aatcgaagac tcggcaggtg ctcgggcggc gctcgtagac
    10801 ggtgcagccc tgctcgccca ggtgcacgca ctccccgttg gccttcttgc gcaggaaccg
    10861 cttgccatcc cggatctcga cgtcgagacc ctcggcggaa tcgaccggca gcaacggtgt
    10921 gtcggcgtat tcgatgcaac acgcgcggca ggaccggcaa ctcaccgtgc tcgtcatcgc
    10981 gacgagatcc gcccggtcgc cggtcacctt gaaccgcgag tacgcgagta tctgcttgcg
    11041 gcgagccgcc acgaaccgct ccacgtcgag tcgtcgatgc ccgctcattt ccctgtcgct
    11101 tcaggtcccg aaaaggttcc cgcactccgc ctcaccggta tcactgcacg ttccttgtac
    11161 agcgtggtcg gacgcgaatc cagccccgcc cggccgccgc ctgagcctcg tgccccgcgc
    11221 ggtgcgtgtg ctggagaccc gagcggcccc gaatcgaatc ccgctcgacc tgcggcggca
    11281 accccggctt ccaaaatgcc ggccgccggg gtatgcttcc gaatagttcg atggttatcg
    11341 aattatgaaa gaacccgcgg tcttgagggc actcgccgca ctgtcgcagg ccagccggct
    11401 gcgcgtcttc cgcgtcctcg tcgtggccgg ccaggcgggc gccacgccca ccgcgatcgc
    11461 caggcggctc aaggtggcgc cggcgacgct gtcgttccac ctcaaggaac tggcgaatgc
    11521 gggtctcgtc agccagcagc gcagcggacg caacctgatt taccgggccg actacgggtc
    11581 gatgaacgac ctcctcgcct acatgacgga gaactgctgc cagggcgagg actgcgggtc
    11641 gggtccggcg cgagcatgca agtcatgacc ggttgccgat gacgaccaac gtactcatcc
    11701 tctgcacgca caactcggcc cgcagcgtgc tggccgaggc gatgctcaat gagtacgcct
    11761 cccgctatgg cagggacgtc cgcgccttca gcgcgggcag cgcgccaagg gggcgcgtca
    11821 acccactcgc gctggcggta ctcgccgatg cgggcgtctc gacgcaggac ctccacagca
    11881 agggctggga ggaattcgcc cggccggact ctcccaggat gcgcgccgtg atcaccgttt
    11941 gcgacagcgc ggctggagag acctgcccga tctggccggg cggcccggtt caggtccact
    12001 ggggctatcc ggacccgtcg gaagcgccgg gcgacgaggt cgcgcgccgc accgcgtttg
    12061 acctcactcg ccaggcgatc ggctatcgga tgctgcaact gcttcgactg cccttcgagt
    12121 cgctggacga cgcggagctg cgggacgcgc tgctcgagat cgcgcggtcg taggaggcct
    12181 cgatgacccc cgtcgcaagc cggctctcct tcctcgaccg ttacctgacg gcttggatct
    12241 tcgccgcgat ggcgctcggc atcgggatcg gctggttcgt ccctgacgtg gagacggtga
    12301 tgaatcgctt caccgtcggc acgaccaacg tcccgatcgc cctcggcctg atcctgatga
    12361 tgtacccgcc attcgcgaag gtccgctacg aggacttgcc cgacgtcttc cgcgacagga
    12421 aggtgctcgc gctgtcgctc gtccagaact ggctggtggg cccggtgttg atgttcctgc
    12481 tggcgatcac cttcctcgcc gacaagcccg agtacatggc aggactgatc ctgatcggcc
    12541 tggcgcgatg catcgccatg gtgatcgtct ggaatgaact ggccggcggc tcgacggagt
    12601 acgccgcagg gctggtggcg ttcaattcga tcttccaggt gctgttctac agcctctacg
    12661 cctgggtgtt cgtcaccgtg ctgcccccga tgttcggact gcacggttcg gtagtggaca
    12721 tctcgatcgc ccagatcgcg aagagcgtgt tcatctacct gggcatcccg tgcattgccg
    12781 ggttgctcac ccgggtcgtc atgctccgct tcgtcagcaa ggactggtac caccagcact
    12841 tcgt
//
LOCUS       DAOMFD010000049        12762 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1216_length_12762_cov_36.341623, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000049 DAOMFD010000000
VERSION     DAOMFD010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12762)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1216_length_12762_cov_36.341623"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="PL152_04565"
     CDS             <1..573
                     /locus_tag="PL152_04565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HQR48585.1"
                     /translation="GKGTGMGLAMVHGIVHEHDGHVVVESSPGHGARFRVLFPALTGA
                     AQHPKGEPDSGGKVRAARPVLAGSVLVVDDEESVGEFMRELLESWGLRATSTPSPEAA
                     LELLQAAPARFDVVITDQSMPRLTGLRLARRIHEIRPDLPVVLYTGHGEGLSGDEVDA
                     ARLCAVIRKPVDPTLLSQTLARCLQARSPA"
     gene            739..1935
                     /locus_tag="PL152_04570"
     CDS             739..1935
                     /locus_tag="PL152_04570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR48586.1"
                     /translation="MKRLDTIALGALIAAYALGPLATQIVTPAVPFVHRDLAVPMAAA
                     QALVSLAFVFIAAATLVYGPLSDRHGRRPVILVGTSLFCVGSLVAAAAPGAAWLIVGR
                     CLQSAGSAAALSLTRTVIHDVYGRERSTRVLAQLTTVMIFVPMLAPALGGVLLDRLGW
                     RSVFSACLAVGLVALSLLGVFLPESRRAQAGATGLADAFASYRALLADRDYRAPTLHF
                     AWVMAAVFATQAAIPYLVIEVLGRSATEYGAWFGVACLAYIGGNQFTARWGQRYDARQ
                     LVRASGFGCLAAALVGAVGIQVAGVTMLTLFVPTIALYFCAAVGIAPVQAEAVAAQPA
                     RSGAASGLLTATQMVIGAASVQAIGFAHDGTSRPLLVALVACSAAAILSYGAVSRRAR
                     LAAREC"
     gene            2157..2654
                     /locus_tag="PL152_04575"
     CDS             2157..2654
                     /locus_tag="PL152_04575"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HQR48587.1"
                     /translation="MRLGDGRLVTIRPIRPEDEDRDHEFLDHLSPESRYQRYFGLMGH
                     LDPALIDRLARADFDQSLALVATVTDAEGERQVAIASYAILPGGRTAEFAIAVDDAWQ
                     EQGLGQELLGSLIAAARDAHRLKRLEGVTMADNHRMLRLARRTGFSLRRDSGDARVVK
                     MERAL"
     gene            complement(2989..3879)
                     /locus_tag="PL152_04580"
     CDS             complement(2989..3879)
                     /locus_tag="PL152_04580"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HQR48588.1"
                     /translation="MAHPRPASIRHLQVFCVAAKQLSFKKAALELHLTPSAVSHRIRE
                     LEGSLGVPLFERRTRAIDLTDAGRALFAEIAPLLDAVEQSVAHVTRHLQRQRLRVAVP
                     QFFASEALVTRLPGFSQRWPQIELRIDSMDARPTVHPQGADASILIAAHPPEGVRSIA
                     LFELVLAVMASPGYCAALGPLSGAIPDDAVLLVHRSWPDMWERWSAASAIRLPNAPQV
                     VEFDTMFALLRAAEGGIGMALVPTHLGDEWLRSGALVRLGCADQPSGEWYWFAARGDD
                     LKRAEVRAFRDWVVTEFGTP"
     gene            4098..6908
                     /locus_tag="PL152_04585"
     CDS             4098..6908
                     /locus_tag="PL152_04585"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR48589.1"
                     /translation="MTPLPQSSEATPRRERFSSGWTGALAVGLFAYVLVAMVLLLFDG
                     KGSRALDIFNLYSDSPPSIGVAILLAAAARHAQDPDTRRTWSLLSAALAFYSIGNLFH
                     STYWLFDVDPFPSVGDVFFLGFYPLVFGAVLTVIRSAAVRVQWARLGLDATILMLGFG
                     AFFWYCVIAPTAAAQHEPDVLKYVLAQSYIALNCITLLACGVLLMHSGATPIQRRALM
                     LLTLGFSAMSFADIIWAMSKVAGSYLPGGLSDTIYLSCYIWLAAAAREQLRNAPAAPR
                     APSAFSNALIQGMPYIAMMISFLVLVYVESSTSASPVNMMTIIIFVLTSLVMARQGVL
                     SRDQLLLRERRAAGIVEARYASLIKNASDVIMIAGSDGQLRFASPAAERTFGIHPDAL
                     VGRNLHDLWSDGDRDRMVAFLAELAATRGRVVGPIELAVETGERRSTLECVGSNLTDD
                     PAIGGLALNFRDVSERKALEEQLRKLAFHDPLTLLANRSLFWNRVEHALALSQRSRQR
                     VAVMFLDLDNFKNVNDSMGHDAGDRLLQAAAQRLIMSTRPSDTVARLGGDEFAILLEG
                     IGTETDIEHIAAPITEAFNRPLLVDGRETDASASIGVACSQPGDDAEQLLRNADIAMY
                     HAKASGKARFAVFQSHMQEQLHDRLRLEQDIDHALARDEFFLEFQPVVDLTRRELLGV
                     EALVRWKHPHRGIVMPGAFIPIAEESDRIVELGRRVLLDACTQVRSWCQSVSAGDGLR
                     VAVNISGRHLQQGNLVDDVRYALEVSGLDPGSLVIELTEGTVMQNTDVNLERFRALKA
                     LGVRLAIDDFGVGYSSLSYLHRFPIDILKIDRAFVSRLTEQDGGPELARAVVMLGTTL
                     GLETVAEGIENEAQAATLLDLGCVAGQGFLFASSMTLENVARTMYMTRRARLRAERAS
                     HTDLTATGRFRVQETWPRSVAS"
     gene            7078..7617
                     /locus_tag="PL152_04590"
     CDS             7078..7617
                     /locus_tag="PL152_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48590.1"
                     /translation="MSEKRPTSVQPPAPANSPSPAGKSPALENTSGRVTFDSRGNAKW
                     EWRADDGGFHTDASTSMVRSLEPQGLALESTTRIQRLIAGTADKDKADAPSAGKSEPW
                     SDVRGHSTASMRIKAAKAAQSRVPGHGGEASGFDPYNSGRSVSHVRKAARRPVAKPAA
                     KPAEPERKGLLARLTGRKP"
     gene            complement(7696..8016)
                     /locus_tag="PL152_04595"
     CDS             complement(7696..8016)
                     /locus_tag="PL152_04595"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR48591.1"
                     /translation="MTCWTAWIGDTVAGMGALKDLGDGTGEVKSMRTHPAFLRRGVAA
                     ALLEHIIGVARARGMRRLSLETGSGPAFEPALELYRRRGFVDGEAFAQYERSAFNQFL
                     HLSL"
     gene            8032..8157
                     /locus_tag="PL152_04600"
     CDS             8032..8157
                     /locus_tag="PL152_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48592.1"
                     /translation="MEPVESKDAAGRRIRVHAFEVQANEFPRLRAGQVVLADFNV"
     gene            8397..8987
                     /locus_tag="PL152_04605"
     CDS             8397..8987
                     /locus_tag="PL152_04605"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR48593.1"
                     /translation="MPGRIGVQPATLAETDDVASVLAEAFFNDPTWSWAFPDPAARLR
                     YWRVCVAEALRYPWVLRCDGYEAVAVWIPPDGTEFSTECDARLPQLLADLAGPRAADV
                     GELIRRFGEAHPRREPHYYLSLLGTADHHRGRGLGMALLRENLARVDAERMPAYLESS
                     NPRNNHLYESVGFVSVTSFRAPGDGPFVTGMWRSSA"
     gene            9037..9597
                     /locus_tag="PL152_04610"
     CDS             9037..9597
                     /locus_tag="PL152_04610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48594.1"
                     /translation="MKDLDMLNTSGVVIFGPGSEWLWTMLQFVALSVTFYAIYRQLRL
                     QQQEIRDNTKVLRAEAHHNCVLLAQRPWEFLLRDEGLARVVKRAYESPEALSDVEWER
                     ASNYMYMQFNAWEYVYYQDKDGSIPRQLMVGTDAYHKSIIASKPGYVRFWREFGHTFD
                     DPFRSYVTAEFARRAAANEARGVTGP"
     gene            9594..>9801
                     /locus_tag="PL152_04615"
     CDS             9594..>9801
                     /locus_tag="PL152_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48595.1"
                     /translation="MKHKRIVVTHYGGADALQVVEEQRPEPKPGEVRVRVLAAGVALP
                     DVLAREGAHPETPRVPYTPGWDLVG"
     assembly_gap    9802..9901
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9902..10659)
                     /locus_tag="PL152_04620"
     CDS             complement(<9902..10659)
                     /locus_tag="PL152_04620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48596.1"
                     /translation="MSLRTLVGRLRHHDWTAVAIELLIVVVGVFIGLQVSNWNEERKE
                     TARGDEYLRRLGVELRGDSRELEQISAFWTKVDDYGAAAIAHAEDGTLLQGSAWQTLL
                     AYYQASQVWPYRKSDVTFQEIRSNGDLLLIRDPGLRERISRHYSAAAGSQVVEVLGLI
                     PRYREDVRGMTPWKIQQYIWSNCYESERANQALKDCESPVSESEALAVLERYRQSSTL
                     TDELRFWMVNVGNGLMLMSGIRAEVDRLADEVEHA"
     gene            complement(10742..12460)
                     /gene="polX"
                     /locus_tag="PL152_04625"
     CDS             complement(10742..12460)
                     /gene="polX"
                     /locus_tag="PL152_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HQR48597.1"
                     /translation="MTVHNAEIAAIFAELADLLEIREDNPFRIRAYRNAAQFIRGHGR
                     SMSELVAAGADLSALPGIGKDLAAKIETIVSSGRLPLLELVRAKVPAPLVEMTRIEGL
                     GPKRAKALYRALRIRSLEDLQRAARSGRIREIKGFGARTEQLIAERAARVAKAEKRIR
                     LADADEIAGPLVEYLRAGEAAKEVVVAGSYRRRRETVGDLDVLVSATRAAAAMDRAVH
                     YEDVAEVVAHGPTRATVRLRNGLQVDIRVVPPASYGAALYYFTGSKAHNIAVRKIAQA
                     RGLKLNEYGVFRGEERVGGRTENEVFDAVGLPYIPPELREDRGEIGAARRKRLPVLVT
                     QGDIRGDLHCHTRATDGRDTIEAMAKAAAAMGYEYVAINDHSQRVTMAHGLDRRRLMT
                     QVRAIDRLNEKLDGIVVLKSVEVDILDDGRLDLPDTVLKELDFTVCAIHYGLDRSREQ
                     QTQRVLRAMDNPYFTILAHPTGRLINAREPYDIDLERVFEAAKERGRILELNAHPDRL
                     DLDDRHCRMAKEAGVRVAISTDAHRASDLALMRYGIGQARRGWLEPADVVNTRGLKEL
                     RKLLRP"
     gene            complement(12538..12696)
                     /locus_tag="PL152_04630"
     CDS             complement(12538..12696)
                     /locus_tag="PL152_04630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48598.1"
                     /translation="MNVSLQGARSEARLNWDQLEERRKASQARSARVFLAALVVFAGY
                     MLWKHYTG"
BASE COUNT         1946 a         4500 c         4204 g         2012 t
ORIGIN      
        1 ggcaagggca ccggcatggg gctcgccatg gtgcacggca tcgtgcacga gcacgacgga
       61 cacgtggtgg tcgagagcag tccgggtcac ggggcacgat tccgtgtcct gttccccgcg
      121 ctgaccggcg ctgcgcagca cccgaagggt gagcccgaca gcggcggcaa ggtgcgcgcg
      181 gctcgaccgg tcctggccgg atccgtcctg gtcgtggacg acgaggaatc ggtcggcgag
      241 ttcatgcgcg aactgctcga gagctgggga ctgcgcgcca cgagcacgcc gagcccggag
      301 gcggcgctcg aactgctgca ggcggcgccg gcgcgcttcg acgtcgtgat cacggaccag
      361 tccatgccac ggctcacggg gctcaggctt gcccggcgca tccacgagat ccggcccgac
      421 ctgccggtgg tcctctacac gggacacggc gagggcttgt ccggcgacga ggtcgatgcc
      481 gcgcgcctgt gcgccgtcat ccgcaagccc gtcgatccga cgctgctcag ccagacgctg
      541 gcgcgctgcc tgcaggctcg cagtcctgcc tagcccgcgg atccgggcac cgcgagaacc
      601 acgccgttcg cgcgagcggc ctggccgata cacatcgata caaatgaggc gccgacggaa
      661 ataggactgt gacgcgaccg ccctaggctc tactccagcg gatcggcaac acatgaggcc
      721 gccgcacgga gagtagccat gaagcgcctc gacacgatcg ccctcggtgc actcatcgcc
      781 gcgtatgcgc tcggcccgct cgccacgcag atcgtgacgc ccgccgtgcc gttcgtgcat
      841 cgggacctcg ccgtgccgat ggccgcggca caggctctgg tgagcctggc cttcgtgttc
      901 atcgccgccg cgacactggt gtacggcccg ctgtccgatc gccatgggcg gcgaccggtc
      961 attctcgtcg ggacgtcgct cttctgcgtg ggcagcctcg ttgcggccgc agccccgggc
     1021 gccgcgtggc tcatcgtcgg ccgctgcctg cagtccgcgg gcagcgcagc ggcgttgtcc
     1081 ctgacccgca ccgtgattca cgacgtctat ggcagggagc gttcgacgcg ggtcctggcg
     1141 cagctcacga ccgtgatgat cttcgtgccg atgctcgcac ccgcgctcgg tggcgtgctg
     1201 ctcgatcgac tcggctggcg ctccgtcttc agtgcgtgcc tggcggtcgg gctcgtcgcg
     1261 ctgtcgctgc tcggcgtatt cctgcccgaa agtcgacgcg cgcaggctgg cgcgaccggg
     1321 ctcgccgatg cgttcgcgag ctatcgggcg ctcctcgccg atcgcgacta tcgcgcgccg
     1381 acgctgcact ttgcctgggt aatggccgcc gtcttcgcga cgcaggccgc catcccgtac
     1441 ctcgtcatcg aggtgctggg tcgcagcgcg accgagtacg gcgcatggtt cggtgtcgcg
     1501 tgcctggcct acatcggcgg caaccagttc accgcacgct gggggcagcg ctacgacgca
     1561 cggcaactgg tccgcgcttc aggcttcggc tgtcttgccg ccgcgctggt cggcgccgtc
     1621 ggcatccagg tcgccggtgt cacgatgctt acgctgttcg tgcccacgat cgcgctctac
     1681 ttctgcgccg ccgtcgggat cgcaccggtg caggcagaag cggtcgcggc ccagccggcg
     1741 cgttccggcg cggcttccgg cctgctgacg gcgacgcaaa tggtcatcgg cgccgccagt
     1801 gtccaggcca tcggcttcgc gcacgacggc acgtcacgac cgctgctcgt cgcgctggtc
     1861 gcttgctcgg cggccgcgat cctgtcgtac ggggccgtgt cgcgacgcgc acggctggcg
     1921 gctcgcgagt gctagggggc accgccatgg cgccgatccc gcgccccggc cgccgtttat
     1981 gaatgagatt ccctcatgca tcaggtaaga aaaccgatgt tgcggcgcag cattaaccgc
     2041 ctagactggc ttgaggccgg acttgagtcc gggccgcatc ttccgcagag cacagcatgg
     2101 ccgtaccgga aaaactagtc aggacagaga cttgcgtacc gacgccgggg cgtctggtgc
     2161 ggctcggcga tggccggctg gtcacgatcc ggccgatccg gccagaggac gaggatcgcg
     2221 atcatgaatt cctcgaccac ctgtctccgg agtcgcgata ccaacgctac ttcggcctga
     2281 tggggcacct cgacccggcc ctcatcgatc gcctggcgcg ggctgacttc gaccagagcc
     2341 tggccctggt tgcgaccgtg accgacgccg agggagagcg ccaggtcgcc atcgcgagct
     2401 acgcgatcct gccgggtggg cgcaccgccg agttcgcgat cgccgtggac gatgcgtggc
     2461 aggagcaggg cctgggacag gagcttctgg gctcgctcat cgcagcggcc cgcgacgcgc
     2521 atcgcctgaa gcggctggaa ggcgtaacga tggccgacaa ccaccggatg ctccgactcg
     2581 cccgtcgcac tggcttcagc ctgcggcgcg actccggcga cgccagggtc gtgaagatgg
     2641 agcgagcgct ctgatccgct gcgcgccacg cggcccgggc cacccattgg aaccgagact
     2701 gctgaacact ggaggtttgc ttggatacct acgtcgtcaa cagtgactac ctgctcgccc
     2761 tcgacgtgct cctcatggcg ttcgccggga cgctcgcact ggacgacggc aggtcgcgtg
     2821 tcgtccggtg gtggaatgca cacggcgcga gcgcgatcgc tcgcgatgca aagccggcgg
     2881 ccaccattcc gccggacggc gacggtgagc agatctatcc cgagtggctc gatcgcatcc
     2941 tgagcgccac gccgccgggt gcactcagtg ccggcaggcg acaggccctc aaggagtccc
     3001 gaactccgtc accacccagt cgcgaaacgc gcgaacctcg gcgcgcttga gatcgtcccc
     3061 ccgggcggcg aaccagtacc actcgccgct cggctggtcc gcgcaaccga ggcgcaccag
     3121 ggctccgctg cgcaaccact cgtcgccgag gtgcgtcggc acgagcgcca tcccgatgcc
     3181 gccctcggcg gcccgcagca gcgcgaacat cgtgtcgaac tcgacgacct gcggcgcgtt
     3241 cggtagacgg atggcacttg cggctgacca ccgctcccac atgtccggcc agctgcgatg
     3301 cacgagcagc accgcgtcgt ccggaatcgc gcccgacagc gggccgagcg cggcgcaata
     3361 gcccggcgat gccatcaccg cgagcacgag ctcgaacaat gcgatcgagc gcacgccctc
     3421 ggggggatgg gccgcaatca ggatcgacgc gtcggcgccc tggggatgga cggtcgggcg
     3481 cgcgtccatc gagtcgatgc gcagctcgat ctgcggccag cgctggctga agccgggcag
     3541 gcgggtcacg agcgcctcgc tcgcgaagaa ctgcggcacc gcgacgcgca gacgctgccg
     3601 ctgcaggtgt cgcgtcacgt gcgcgaccga ctgctccacc gcgtcgagca aaggcgcaat
     3661 ctccgcgaac aacgcgcgac cggcgtccgt caggtcgatg gctcgcgtgc gacgctcgaa
     3721 cagcggcacg ccgaggctgc cctcgagttc gcggatgcgg tggctcaccg cggacggcgt
     3781 caggtgcagt tcgagcgccg ccttcttgaa gctgagttgc ttcgcggcga cgcagaacac
     3841 ctgcaggtgt cggatcgatg cgggtcgcgg gtgggccatg gcgggttcct gccaccccca
     3901 tgccacgcaa cctcggggcg aatgtcaagg cgcggccgcc aacccctccg gccggcgggg
     3961 cccggccgag gtgtgcgcca cgtcacaagg tcgggagtcg ataccggacc cctttcacgg
     4021 atgcgcggca gtggcttctg gctacatgtg cggggtaaac aacagtcgcc gcgcacggga
     4081 gacgcgcgcc gcagaacatg acgccattgc cgcagtcgag cgaggccaca ccgcgacgcg
     4141 aacggttcag ttccggctgg accggggcgc tcgccgtcgg gcttttcgcc tacgtcctgg
     4201 tcgcaatggt gctgctgctg ttcgacggca agggatcgcg ggccctcgac atcttcaacc
     4261 tctattcgga ctcgccaccg agcatcggcg tggccatcct gctcgcggcc gccgcccgcc
     4321 acgcccagga ccccgacacg cggcgcacct ggtcactgct ctcggcggcg ctcgctttct
     4381 acagcatcgg caacctgttc cactcgacct actggctgtt cgacgtcgac ccgttcccgt
     4441 cggtcggcga cgtcttcttc ctcggcttct acccgctcgt cttcggtgcg gtgctgacgg
     4501 tgatccgctc cgccgccgtg cgcgtgcagt gggcccggct cggcctggac gccacgatcc
     4561 tgatgctcgg cttcggcgcg ttcttctggt actgcgtgat cgcgccgacg gccgccgcgc
     4621 agcacgagcc ggacgtgctc aagtacgtgc tggcgcagag ctacatcgcg ctgaattgca
     4681 tcacgctgct cgcgtgcggc gtgctgctga tgcactccgg cgccacgccc atccagcgcc
     4741 gcgcgttgat gctgctcacg ctcggcttct cggcgatgtc gttcgccgac atcatctggg
     4801 ccatgtcgaa ggtcgcgggc agttaccttc ccggcggcct gtcggacacc atctacctgt
     4861 cctgctacat ctggctcgcg gccgccgcgc gcgagcagct gcgcaatgca cccgctgcac
     4921 cgcgcgcgcc gagtgcattc agcaacgcgc tgatccaggg catgccgtac atcgcgatga
     4981 tgatctcgtt cctggtgctg gtgtacgtcg agagcagcac ctccgcgagc ccggtcaaca
     5041 tgatgaccat catcatcttc gtgctcacct cgctcgtgat ggcgcgccag ggcgtgctgt
     5101 cgcgcgacca gctgctgctc agggagcgac gcgccgccgg tatcgttgag gcccgctacg
     5161 cgtcgctcat caagaacgct tccgacgtga tcatgatcgc cggctccgac gggcagctgc
     5221 gcttcgcgtc gccggccgcc gagcggacct tcgggatcca cccggacgcg ctggtcggcc
     5281 gcaacctgca cgacctctgg tcggacggcg accgcgatcg catggtcgcg ttcctcgccg
     5341 aactggccgc cacccgcggg cgggtggtcg ggccgatcga gctggcggtc gagaccggcg
     5401 agcggcgcag cacgctcgag tgcgtaggca gcaacctcac ggacgacccg gccatcggcg
     5461 gcctggcgct gaatttccgc gacgtgagcg agcgcaaggc gctcgaggag cagctgcgca
     5521 agctggcgtt ccacgacccg ctgacgctgc tcgcgaaccg gagcctgttc tggaaccgcg
     5581 tcgagcacgc gctcgcgctg tcgcagcgct cccggcagcg cgtcgcggtc atgttcctcg
     5641 acctcgacaa cttcaagaac gtcaacgact cgatgggcca cgatgccggc gaccgcctgc
     5701 tgcaggcggc ggcgcagcgg ctgatcatgt ccacgcgccc gtccgacacg gtcgcgcgcc
     5761 tcggcggcga cgagttcgcg atcctcctcg agggcatcgg caccgagacc gacatcgagc
     5821 acatcgccgc gcccatcacc gaggcgttca accggccgct gctggtcgac ggccgcgaga
     5881 ccgatgcctc cgccagcatc ggcgtcgcct gctcgcagcc cggcgacgac gccgagcagc
     5941 tgctgcgcaa cgcggacatc gccatgtacc acgccaaggc gtccggcaag gcgcgcttcg
     6001 cggtgttcca gtcgcacatg caggagcagc tgcacgaccg cctgcgactc gagcaggaca
     6061 tcgaccacgc gctcgcacgc gacgagttct tcctcgagtt ccagccggtc gtggacctga
     6121 cgcgccgcga attgctcggc gtcgaggccc tggtccgctg gaagcacccg catcgcggca
     6181 tcgtcatgcc cggcgcattc atcccgatcg cggaggagtc ggaccggatc gtcgaactcg
     6241 gccgccgcgt gctcctcgac gcctgcacgc aggtgcggtc gtggtgccag tcggtttccg
     6301 ccggggacgg cctgcgcgtc gcggtcaaca tatccggccg tcaccttcag cagggcaacc
     6361 tggtcgacga cgtgcgttat gcgctcgagg tctccgggct cgaccccggc agtctcgtga
     6421 tcgagctcac cgagggcacg gtcatgcaga acaccgacgt gaacctcgag cgcttccgag
     6481 cgctgaaggc gctcggcgtg cggctcgcga tcgacgattt cggcgtcggc tactcgtcgc
     6541 tctcctacct gcaccgcttc ccgatcgaca tcctgaagat cgaccgcgcg ttcgtgagcc
     6601 ggctcaccga gcaggacggc ggccccgagc tggcgcgcgc ggtcgtgatg ctcggcacga
     6661 cgctcgggct cgagacggtc gccgagggca tcgagaacga ggcgcaggcc gcgacgctgc
     6721 tcgacctcgg ctgcgtggcg ggccagggct tcctgttcgc cagctcgatg accctcgaga
     6781 acgtcgctcg cacgatgtac atgacgcgcc gcgcccgcct gcgtgccgag cgcgcctcgc
     6841 acaccgacct tactgcgacc ggtcgcttcc gggtccagga gacctggccg cgttccgtgg
     6901 ccagctgagt ggcgggcagc gcccggcatc gcaagcctgg cgcccgtaac cgcgatccag
     6961 ttcacagttt gcgcagcccc cgacgcccgg cttttggggc ggaacccggc ggcatacata
     7021 atgccgacgt gtgatcgagc aagtgccacg cgaagcgaca gccagtcgag acctgacgtg
     7081 agcgagaaac gtcccacgag tgtgcagccg ccggcccccg cgaacagtcc gtcgccggcc
     7141 ggcaagagtc ctgcgctcga gaatacctcc ggtcgtgtca ccttcgactc gcgcggcaac
     7201 gcgaagtggg agtggcgcgc ggacgacggc ggattccata ccgatgcgag cacgtccatg
     7261 gtgcggtccc tggagccgca ggggctggcg ctcgagtcga ccacgcgcat ccagcgcctg
     7321 atcgccggca ccgcggacaa ggacaaagcc gatgcgccgt cggccgggaa gtcggagccc
     7381 tggtccgatg tccggggcca ttcgaccgcg agcatgcgca tcaaggcggc gaaagccgcg
     7441 cagtcccggg tgcccgggca cggcggcgag gcgtctggct tcgacccgta caacagcggg
     7501 cgcagcgtga gtcacgtacg caaggcagct cgccgcccgg tagccaagcc tgccgccaag
     7561 cccgccgaac ctgaacgcaa gggcctgctc gcgcgcctga ccgggcgcaa gccctgatcg
     7621 cgcggtctgt cgttaccaac cagtcacgcc cgggcgcgcg cgccggccga gtccgcgcat
     7681 ccatgccgcc gctggttaca gcgacaggtg caggaactgg ttgaacgcac tgcgctcgta
     7741 ctgtgcgaac gcctcgccgt cgacgaagcc acgtcggcga tacagttcca gggctggctc
     7801 gaacgccggc ccgctgccgg tctcgaggct gagccgcctc atgccgcgcg cccgggcgac
     7861 gccgatgatg tgttcgagca gcgccgccgc gactccgcgc cgcaggaacg ccgggtgcgt
     7921 gcgcatggac ttgacctccc cggtgccgtc acccaggtcc ttcagcgctc ccatgccggc
     7981 gaccgtgtcg ccgatccacg ccgtccagca agtcatgccg ggacctttga gttggagccg
     8041 gtcgagagca aagacgctgc cgggcggcga attcgcgtgc atgccttcga ggtgcaggcg
     8101 aacgagttcc cgcgtctgcg cgccggacag gtcgtcctcg cggatttcaa cgtctgatgg
     8161 actgatcggc gcctcccgcc gctgggtccg gcatctctcg aggttaccgc aagcctgcgg
     8221 cgattcatcg aataatccaa cgcacacagc ggcgtgccga ctcggtcgcc gcggaagggt
     8281 atagtccggc cgacgagcca cgcgtgcagg gggacgtggc catgcccggc gaccggacga
     8341 caccgatcgg gtggatacgc ccgtctcgcc atcgcaagac ggcgccggac accgacgtgc
     8401 ccggccgaat cggcgtccag ccagccaccc tggcggaaac ggatgacgtt gcgtccgtcc
     8461 ttgcggaagc gttcttcaac gatccaacgt ggtcgtgggc gtttcccgat cccgccgcac
     8521 ggctgcgcta ctggcgtgta tgcgtcgccg aggccctgcg ctatccgtgg gtcctgcgct
     8581 gcgacgggta cgaagcggtc gcggtgtgga ttccgccaga cggcacagag ttctcgaccg
     8641 agtgcgacgc gcggcttccg cagctgctcg cggatctcgc aggacccagg gccgccgacg
     8701 tcggcgaact catacgtcga ttcggcgagg cgcaccctcg tcgcgaaccc cactactacc
     8761 tgagcctgct cggcacggcg gatcaccacc gtggtcgtgg gctgggcatg gcgctgctgc
     8821 gcgagaatct cgcgcgcgtc gacgccgagc gcatgccggc ctacctggaa tcgagcaacc
     8881 cccggaacaa ccacctttac gagtcggtcg gcttcgtgag cgtcacgtca tttcgggcac
     8941 ccggtgacgg tccgttcgtg accggcatgt ggcgttcgag cgcctgacgc aggcccccga
     9001 cgaccgctcg aattgcagca atccatcgct cgaaccatga aggacctcga catgctcaac
     9061 accagcggcg tcgtgatctt cggccccgga tcggaatggt tgtggaccat gctccagttc
     9121 gtggcactga gcgtcacgtt ctacgcgatc tatcgccagc tgcgcctgca gcagcaggag
     9181 atccgcgaca acaccaaggt gctgcgcgcc gaagcccatc acaactgcgt gctgctcgcc
     9241 cagcgaccgt gggagttcct gctgcgcgac gagggactgg cgcgcgtcgt gaagcgggcg
     9301 tacgagtcac cggaagcgct gagcgacgtc gagtgggagc gggccagcaa ctacatgtac
     9361 atgcagttca acgcctggga gtacgtctat taccaggaca aggacggatc catcccgagg
     9421 cagctgatgg tcggcaccga cgcgtaccac aagtccatca tcgcctcgaa gcccggctac
     9481 gtgcgcttct ggagggaatt cggccacacg ttcgatgacc cgttccgctc ctacgtgacg
     9541 gcggagttcg cgcggcgagc ggccgccaac gaagccaggg gagtgaccgg tccatgaagc
     9601 acaagcgcat cgtcgtcact cactatggcg gtgccgacgc actgcaggtg gtcgaggaac
     9661 aacgccctga gccgaagccc ggcgaagtgc gggtgcgagt gctcgccgcg ggcgtggcac
     9721 tgcccgacgt gctggcccgc gaaggcgcgc atcccgagac gcccagggtg ccgtacacgc
     9781 cgggctggga cctggtcggc annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9901 ngcgtgctcc acctcgtccg ccagccggtc gacctcggcg cgaatgcctg acatcagcat
     9961 gaggccgttg ccgacgttca ccatccagaa ccgcagctcg tcagtcagcg tcgacgactg
    10021 gcggtagcgt tcgagcaccg cgagcgcctc ggattcgctc acgggtgact cgcagtcctt
    10081 caacgcctgg ttggcccgct cggattcgta gcagttcgac cagatgtact gctggatctt
    10141 ccacggcgtc atcccgcgca cgtcttcccg gtaacgggga atcaggccca gcacctcgac
    10201 cacctggctg ccggccgcgg cgctgtagtg ccgtgaaatc cgctcgcgca atcccgggtc
    10261 gcggatcagc agcagatcgc cattcgagcg gatctcctgg aaggtgacgt cggacttccg
    10321 gtacggccac acctggctcg cctggtagta cgcgagcaac gtctgccacg ccgagccctg
    10381 caacagcgtc ccgtcctccg catgggcgat cgccgccgcg ccgtagtcat cgaccttcgt
    10441 ccagaaggcg gagatctgct cgagctcgcg cgaatccccc cgcaactcga cgcccagccg
    10501 gcgcaggtac tcgtcaccac gggccgtctc cttgcgttcc tcgttccagt tgctgacctg
    10561 caggccgatg aacactccga cgacgacgat gagcaattcg atcgcgaccg ccgtccagtc
    10621 gtggtgccgc agccggccga cgagcgtacg cagggacatg ccgcctcccc ctcaacacga
    10681 gcgagagcga cagtgtagac aggtgcgcgc ccggtgtcgg cacccggtag atccggggct
    10741 gtcacggacg cagcaacttg cgcagttcct tcagcccgcg tgtattgacg acgtccgccg
    10801 gctcgagcca gccacgccgc gcctggccga tgccgtaccg catcagggcg aggtccgatg
    10861 cgcggtgggc gtccgtggag atcgcgacgc gcacccccgc ctccttcgcc atgcgacagt
    10921 ggcgatcgtc gaggtcgagg cggtcgggat gggcgttgag ttccaggatc cgcccgcgct
    10981 ccttcgcggc ctcgaacacg cgctccaggt cgatgtcgta cggttcccgc gcattgatca
    11041 agcggccggt cgggtgcgcg aggatcgtga aatacgggtt gtccatcgcc cgcaggacgc
    11101 gctgcgtctg ctgctcgcga ctccggtcga ggccgtagtg gatcgcgcac accgtgaaat
    11161 cgagttcctt cagcaccgtg tcgggcaggt cgagccggcc gtcgtccagg atgtcgacct
    11221 ccaccgactt gagcacgacg atgccatcga gcttctcgtt gaggcggtcg atcgcacgga
    11281 cctgcgtcat cagccggcgg cggtcgagac cgtgcgccat ggtgacgcgc tgcgagtgat
    11341 cgttgatcgc gacgtattcg taccccatcg cggccgcggc cttcgccatc gcctcgatgg
    11401 tgtcccgccc gtcggtcgcc cgcgtgtggc agtgcaggtc gccgcggatg tcgccctgcg
    11461 taacgagcac ggggaggcgc ttgcggcgcg cggccccgat ctcgccgcgg tcctcgcgca
    11521 attccggcgg tatgtacggc aggccgaccg cgtcgaagac ctcgttctcg gtgcgaccgc
    11581 cgacgcgctc ctcgccgcga aagacgccgt actcgttgag cttcaagccc cgcgcctgcg
    11641 cgatcttgcg gactgcgatg ttgtgcgcct tcgaaccggt gaaatagtac agcgctgccc
    11701 catagctggc cggcggcacg acgcgaatgt ccacctgcag cccattgcgc agccgcaccg
    11761 tcgcacgcgt cggaccatgg gcgaccacct ccgccacgtc ttcgtagtgc accgctcggt
    11821 ccatcgcagc ggcggcccgg gtcgcgctca ccaggacgtc caggtcgccc accgtctcgc
    11881 gccgtcgtcg gtagcttccg gccaccacga cctcctttgc ggcctcaccc gcccggaggt
    11941 actcgacgag cggcccggca atctcatccg catcggcgag acgaatgcgc ttctccgcct
    12001 tcgcgacccg cgcggcgcgc tcggcgatca gctgctcggt tctcgcaccg aagcccttga
    12061 tctcccggat tcggccgctg cgcgcggcgc gctgcaggtc ctcgaggctg cggatcctga
    12121 gggcccgata cagcgccttc gcgcgtttcg ggccgagacc ctcgatgcgc gtcatctcca
    12181 cgagcggcgc ggggaccttc gcgcgcacga gctccagcaa cggcaaacgg ccactggaca
    12241 cgatggtctc gatctttgcg gcaaggtcct tgccgatgcc gggcaatgcc gacaggtccg
    12301 cccctgcagc gaccagctcg gacatgctcc ggccgtggcc gcggatgaac tgcgcggcgt
    12361 tgcgatacgc gcggatgcgg aacgggttgt cctcgcggat ctcgagaagg tccgcgagtt
    12421 cggcgaagat cgcggcgatt tccgcgttgt ggacggtcat gcgcccagcg ccgcgtcgat
    12481 cgcaagcgct gcctcgctac gggcggtgac cctgccgctc ggcggcagcc gcgatcgtca
    12541 gcccgtgtag tgcttccaga gcatgtagcc ggcaaacacg accagtgccg ccaggaacac
    12601 tcgggccgac cgtgcctggg atgccttgcg gcgctcttcc agctggtccc aattcagccg
    12661 cgcttcgctg cgggcaccct gaagactcac gttcatcgct cggcctcccc tcggccagtc
    12721 gtgacatcct tcccgatgcc gaacctagcg cggccgcggg ac
//
LOCUS       DAOMFD010000050        11987 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1367_length_11987_cov_28.868002, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000050 DAOMFD010000000
VERSION     DAOMFD010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11987)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11987)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11987
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1367_length_11987_cov_28.868002"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..385
                     /gene="cysD"
                     /locus_tag="PL152_04635"
     CDS             <1..385
                     /gene="cysD"
                     /locus_tag="PL152_04635"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004752346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HQR48599.1"
                     /translation="HKGESIRVFPLSNWTELDVWQYIYLESIPIVPLYFAKERPVVER
                     DGTLIMVDDDRMPLKDGEVPMMRSVRFRTLGCYPLTGAVESRADTLPAIIQEMLLTKT
                     SERQGRVIDHDQAASMEKKKQEGYF"
     gene            385..>2276
                     /gene="cysN"
                     /locus_tag="PL152_04640"
     CDS             385..>2276
                     /gene="cysN"
                     /locus_tag="PL152_04640"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HQR48600.1"
                     /translation="MAHQSDLIETNIEQYLRAHETKGLLRFITCGSVDDGKSTLIGRL
                     LYESKMLFEDQLAAIEKDSKKWGTQGDEIDFALLVDGLAAEREQGITIDVAYRFFSTD
                     KRKFIVADTPGHEQYTRNMVTGASTADLAIILIDARRGVLTQTRRHSYLVSLIGIHNV
                     VLAINKMDLVDYSEARFRAIEDEYRAFARQIGLTNITAIPLSALRGDNMLEPSDRTPW
                     YHGPTLMGFLETVPIDEDRLQSKPFRLPVQWVNRPHHDFRGFAGTIAAGLVRPGDRIR
                     VLPSGKSSTVSRIVTSEGDLERAVAGQSVTLTLADEIDISRGDVICTADAPGAAADQF
                     ETTVIWMHEEPLFPGRAYTMKIGAKTVTATVTDIKYKVNVNTLEHLATKTLALNEIGV
                     CNLSVDQQIAFDPYVENRETGGYIIIDRLSNNTLGAGLLHFALRRAHNIHMQHMDVDQ
                     SARSRMMGQKPAVLWFTGLSGAGKSTIANLVERKLHAMGRYTYMLDGDNIRHGLNKDL
                     GFTDADRVENIRRIGEVARLMVDAGLIVLTAFISPFRSERRMARGMVKDGEFVEVYVD
                     TPLRIAEERDPKGLYRKARRGEVKNFTGIDSPYEEPENPEVHIDTTELSAEQAAERII
                     VSLKRLG"
     assembly_gap    2277..2376
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2377..2978
                     /locus_tag="PL152_04645"
     CDS             <2377..2978
                     /locus_tag="PL152_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48601.1"
                     /translation="IIGFTFSMALGRYDQRKAYEEEEANAIGTEYLRAEMLPAADAAR
                     VQSLLREYLDQRILFYTTRDEDALSGISAKTSDLQSQMWAAVRGPAAAQPSPLSALAV
                     AGMNDVINTQGYTQAAWWNRIPFAAWALMLAIAICTTLLMGIGARTRDRFPKVLLVLP
                     VVISVAFFMIADLDSPRRGVIRVVPMNLDVLAESLRQPH"
     gene            complement(3065..3544)
                     /locus_tag="PL152_04650"
     CDS             complement(3065..3544)
                     /locus_tag="PL152_04650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48602.1"
                     /translation="MLKFISSLVVGAALVIGSAAAGASGAADSVVGTWTLDLAKSRFD
                     PGPAPKSQTRTYAATADGIDLTVEGVAADGSKVSQHSSYKYDGKDYAITGAADYDTLS
                     LKRIDANRVESEQKKGGKVVGHTVRTVSADGKTMTLDSKGTDSKGGKYKNVMVFDRK"
     gene            3796..6117
                     /locus_tag="PL152_04655"
     CDS             3796..6117
                     /locus_tag="PL152_04655"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HQR48603.1"
                     /translation="MTPEQRGLRFHMRMLMARTLLPLLATFSCAGLQAAETGALAADS
                     VDDTDNGSVSVIVKFRDAHRPGDVADAAADEDVAALAGRTGIGLAHVRSIQPGLRVVK
                     VRAIAAPGSLGVALQRLREDPGVQYAEIDQRRHAHAVLPDDTLFDGPYSGQWYLKNAG
                     DTPSAIDAVTAWDTIRGSTGVIVAVLDTGVRYDHPDLLAAGEGGRLLPGYDFISSAAE
                     SNDGDGRDPDPSDPGDWSSSSDSCGASGSSWHGTRVSGIIAALTNDTMGVAGILWSGW
                     VLPVRVLGRCGGFDSDIVPAMLWAAGIPVSGMPSNPYPARVINLSLGSTGSCPAEYAD
                     AVKQLSDRGVLVVASAGNEGSVVAVPANCPGVAGIAAIRHIGTKVGFSNLGPAVALSA
                     PGGNCVNVDPGLPCLYSIDTTTNLGTTGPAANGYTDRFADANLGTSFSAPIVTGIAGL
                     MAAANGNLTSAQLTARLREGAKPFPAYPADPTDPPLPTCHTPTDANDVQDQCVCTADT
                     CGAGMANARGAVAAALRPIAAITLPGRFSPGQDVVLRGGSSGAACGHALASYAWSVVA
                     GTPPSPIVGANTDTATVKAPSSGSYTVRLTVADDAGRQDVADIVVQPTLATTAAPASA
                     GATACPAPIVAITLSPVLATVQAHGGTIAFSVSVANATDTGVLWYVNDAAGGNATVGT
                     ISTTGFYTAPASAPAAGSVTIKAVPAADASRFAAARVTITAAPVGAGGGSGGSGGGGG
                     GGTLDPVALLALLASRVAHLSRRPQQRGSEAAR"
     gene            6138..7556
                     /locus_tag="PL152_04660"
     CDS             6138..7556
                     /locus_tag="PL152_04660"
                     /inference="COORDINATES: protein motif:HMM:NF013056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HQR48604.1"
                     /translation="MLERIFRLREHGTSVRTELTAGLTTFLTMSYIIVVQPAVLSGSL
                     FGEPTGMDFGAVTTATCLAAALASAIMGLYARYPIAQAPGMGENFFFVFSAIPAAAAA
                     GAAVPWATALGAVFVSGVVFLVLTLAGAREAVMNALSPSMKHAIAAGIGLFIAFIGLQ
                     NAEVVVGDPGTAVTLNPDIGSPDVAVFLVGLLTAAGLHARRIRGSILWGILAAGAVAV
                     GLKLALPLLGDSVMGAPSVANSALATRFELASHVVAAPPSLQPTFLRMDVRGALTLAM
                     APYVAIFLFMVFFDTVGTLVGVAGQAGLMPNGVLPRARQAFVSDATATVVGACLGTST
                     VTSYIESATGVEQGGRTGLTALAVAVLFLVALFFSPVIAMLGAYPPITSPALVLVGAM
                     MLGNIRHLQWDDYTEVLPAFLVLAGIPFTYSIADGLALGLIAHPMVKVLAGRRRETRA
                     ASWIIAALLAGYFVVVRSAASG"
     gene            7564..8931
                     /locus_tag="PL152_04665"
     CDS             7564..8931
                     /locus_tag="PL152_04665"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HQR48605.1"
                     /translation="MKTIIEPFRIKVVEPIRLTTVDERRALLQRAGWNLFAIHADDVI
                     IDLLTDSGTSAMSADQWGAVMRGDESYAGSPSYYRFEAAVRELMNFRHVIPTHQGRAA
                     EAILFSIVGGAGRIVPSNTHFDTTRGNIEATGARAVDLVIHEAHEPDNLHPFKGNMDV
                     ARLEDLLRESGDAIPLVMLTVTNNAGGGQPVSLENIRATAEVAHRHGKPLVIDGCRFA
                     ENAWFIKCREPGQGERAVADIVRDMFAVADGMTMSAKKDAFANIGGWLATNDDALAAE
                     ARNRLILTEGFPTYGGLAGRDLEAIAVGLHEIVDEQYLRYRVRTNEYIGEKLTALGVP
                     IVRPVGGHAVFVDARRFLPHIPPLDYPGQALAVALYEAGGIRACEIGTVMFGMGPDGV
                     ERAADMDLVRLAMPRRVYTQSHADYLVEVFEEVVRRKAELRGFRIVSQPPRLRHFTAR
                     FQPLG"
     gene            complement(8928..10340)
                     /locus_tag="PL152_04670"
     CDS             complement(8928..10340)
                     /locus_tag="PL152_04670"
                     /EC_number="4.1.99.2"
                     /inference="COORDINATES: protein motif:HMM:NF009709.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050371 - tyrosine phenol-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006570 - tyrosine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine phenol-lyase"
                     /protein_id="HQR48606.1"
                     /translation="MSKTVHRTMGQQSGRRSWAEPWKIKMVEPLRMTRREDRARALAD
                     AGYNTFLLRSDDVYIDLLTDSGTNAMSDRQWAGMMLGDEAYAGSRNFYHLEEAIRHWY
                     GYEHVVPTHQGRGAEHLLSQAAISKGQYVPGNMYFTTTRLHQELAGGIFVDVIADAAH
                     DPTDPSPFKGNVDLEKLEKLIDRHGARGIAYVSLAATVNMAGGQPVSMANARSLHALC
                     RKHGVRVFLDATRLAENAFFIQEREPGYADKPIAEIVKEFCSHTDGAWMSAKKDALVN
                     IGGWLAVRDWELFEELRNLVVVYEGLHTYGGLAGRDMEAMAIGIAESMQDDHMRARIG
                     QVRYLAELLQDWDVPMVQPVGGHAIFLDARAFYPQLRQDQFPSQTLAAELYLDSGIRS
                     MERGIVSAGRDPATGEHHYPKLELTRLTIPRRVYTQAHMDVVAESVKAVYDARGAARG
                     LKMVYEPKYLRFFQARFEPL"
     gene            complement(10482..10568)
                     /locus_tag="PL152_04675"
     tRNA            complement(10482..10568)
                     /locus_tag="PL152_04675"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10532..10534),aa:Leu,seq:taa)
     gene            complement(10586..11401)
                     /locus_tag="PL152_04680"
     CDS             complement(10586..11401)
                     /locus_tag="PL152_04680"
                     /inference="COORDINATES: protein motif:HMM:NF019349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HQR48607.1"
                     /translation="MSRQIKRSRPARAAPHRARPARPAGRTARLKPADRGTRSAAFAR
                     IAADLEARILDRSLRPGDPLPSETALAEQYSVHRSTVREAVRRLESAGLIGRAAGTKR
                     LRVTRPGQAATTSSLSRALALDDVTFIELWEAMLAIAPRTAWLAAAHPRPENIERLEF
                     CIAKVETARSAEGAVAGVVEFFGSLAATSANRVLVLAMQPVTKLLAPSLRRVIDRVPQ
                     GRSRIAVAQRCIVEAMRRGDADEAQAWMTRHVQDFRRGYEVAGIALDTRAGDV"
     gene            11461..>11987
                     /locus_tag="PL152_04685"
     CDS             11461..>11987
                     /locus_tag="PL152_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48608.1"
                     /translation="MVDFDFGLGEDLDLLRETVRAFAEQELAPRADAIDRTNQFPRDL
                     WPRLGELGLLGITVEPEWGGAGLGYLAHVVAMEEISRASGSVGLSYGAHSNLCVNQVR
                     RWGSHEQKARYLPKLVSGEHLGALAMSETGAGSDVMGMRLRAEPHGDRYVLNGSKMWI
                     TNGPTADVLVVYATV"
BASE COUNT         1873 a         4008 c         4089 g         1917 t
ORIGIN      
        1 gcacaagggc gagtcgatcc gcgtcttccc gctgtcgaac tggaccgaac tcgacgtctg
       61 gcagtacatc tacctcgaga gcatcccgat cgtgccgctc tatttcgcga aggagcggcc
      121 ggtcgtcgag cgcgatggca cgctcatcat ggtcgacgac gatcgcatgc cgctcaagga
      181 tggcgaggtg ccgatgatgc gcagcgtgcg attccgaacg ctcggctgct atccgctgac
      241 gggcgccgtc gaatccaggg ccgacacgct ccccgcgatc atccaggaga tgctgctcac
      301 gaagacctcg gagcggcagg gccgcgtcat cgaccacgac caggcggctt ccatggagaa
      361 gaagaagcag gaggggtact tctgatggcc caccaatccg acctgatcga gaccaacatc
      421 gagcagtact tgagggctca cgaaaccaag ggcctgctgc gcttcatcac ctgcgggtcc
      481 gtggacgacg gcaagagcac gctgatcggc cggttgctct acgagtcgaa gatgctgttc
      541 gaggaccagc tcgccgccat cgagaaggac tcgaagaagt ggggtacgca gggcgacgaa
      601 atcgacttcg cgctgctggt cgacggcctg gccgcggagc gcgagcaggg catcaccatc
      661 gacgtggcgt accgcttctt ctccaccgac aagcgcaagt tcatcgtcgc ggacacgccg
      721 gggcacgagc agtacacccg caacatggtg accggcgcct cgacagcgga tctggccatc
      781 atcctgatcg acgcgcggag gggcgtgctc acgcagacgc ggcggcacag ctacctcgtg
      841 tcgctgatcg gcatccacaa cgtcgtgctg gccatcaaca agatggacct cgtcgactat
      901 tccgaggcgc gattccgcgc catcgaggac gagtaccgcg cgttcgcgcg gcagatcggg
      961 ctcaccaaca tcacggccat tcccctgtcg gccctccggg gcgacaacat gctcgagccg
     1021 agtgaccgca cgccgtggta ccacgggccg acgctgatgg gattcctcga gacggtgccg
     1081 atcgacgagg accgcctgca gtcgaaaccg ttccgcctcc cggtgcagtg ggtgaatcgt
     1141 ccgcaccacg acttccgcgg cttcgccggc accatcgccg ccggcctggt ccgtccgggc
     1201 gaccggatcc gggtgctgcc gtccggcaag agcagcacgg tgtcccgcat cgtcacgagc
     1261 gagggcgacc tcgagcgagc cgtggcgggg cagtcggtca cgctgaccct ggccgacgag
     1321 atcgacatca gccgtggcga cgtgatctgc acggcggacg cgcctggcgc ggcggccgac
     1381 cagttcgaga ccaccgtgat ctggatgcac gaggagccgc tgttccctgg gcgtgcgtac
     1441 accatgaaga tcggcgccaa gacggtcacg gccacggtca cagacatcaa gtacaaggtg
     1501 aacgtcaata cgctcgagca cctcgccacc aagaccctgg cgctgaacga gatcggggtg
     1561 tgcaacctgt ccgtcgacca gcagatcgcc ttcgatccgt acgtcgagaa ccgcgaaacc
     1621 ggcggctaca tcatcatcga ccgcctctcg aacaacacgc tgggcgcggg actgctgcac
     1681 ttcgcattgc ggcgcgcgca caacatccac atgcagcaca tggacgtgga ccagtccgcg
     1741 cgctcgcgga tgatgggcca gaagccggcc gtgctctggt tcaccggctt gtccggcgcc
     1801 ggcaagtcca cgatcgcgaa cctcgtcgag cgcaaattgc acgccatggg ccgctacacc
     1861 tacatgctgg acggcgacaa catccggcat ggcctcaaca aggacctcgg cttcaccgac
     1921 gcggaccgcg tcgagaacat ccgccgcatc ggcgaggtgg cccgactgat ggtggacgcg
     1981 ggcctgatcg tgctcacggc gttcatctct ccgttccgct cggagcgacg catggcccgc
     2041 ggcatggtga aggacgggga gttcgtcgag gtgtacgtcg acacgccgct caggatcgcc
     2101 gaggagcgcg acccgaaggg cctgtacagg aaggcgcgtc gcggcgaggt caagaacttc
     2161 acgggcatcg actcaccgta cgaggagccc gagaatccgg aggtccacat cgacaccacc
     2221 gagctgtcgg cggagcaggc ggccgagcgg atcatcgtga gcctcaagcg gctcggnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnntgat catcggtttc acgttctcga
     2401 tggcgctcgg tcgctacgac cagcgcaagg catacgagga ggaggaggcc aacgccatcg
     2461 ggaccgagta cctccgcgcc gagatgcttc cggctgccga tgccgccagg gtgcagtcac
     2521 tgctccgcga gtacctcgac cagcgcatcc tgttctacac gacgcgtgac gaggatgcgc
     2581 tctcgggcat ctctgcaaag acgtccgatc tgcagtcgca gatgtgggcg gccgtgaggg
     2641 gcccggcggc cgcgcaaccg tcgccgctca gtgccctcgc ggtcgcgggc atgaacgacg
     2701 tgatcaacac tcagggctat acgcaggccg cctggtggaa ccggattccg tttgccgcat
     2761 gggcgctcat gttggcgatc gcgatctgca ctacgctgct gatgggcatc ggcgccagga
     2821 cgcgcgaccg gttcccgaag gtgctgctgg tgctgccggt cgtgatatcg gtcgccttct
     2881 tcatgatcgc ggatctcgac agtccgcgcc ggggcgtgat ccgcgtggtg cccatgaacc
     2941 tggacgtgct ggccgaatcg ttgcgccagc cgcactaggc gccgaccgtc agggcagcgg
     3001 gccggacacg aagcggcgcc ggacgctcgc gcgccggcgc cgtcgtgagc gacgcgctga
     3061 agtgctactt gcggtcgaac accatcacgt tcttgtactt gcctcccttc gaatcggtgc
     3121 ccttgctgtc gagcgtcatg gtcttgccgt ctgcggagac ggtgcgcacg gtgtgcccga
     3181 cgaccttgcc acctttcttc tgttcgctct cgacgcggtt cgcgtcgatc cgcttgagcg
     3241 acaacgtgtc gtagtccgcc gcgccggtga tggcgtagtc cttgccgtcg tacttgtacg
     3301 acgagtgctg cgagaccttg gaaccgtccg cggcgacacc ttcgacggtg agatcgatcc
     3361 cgtccgcggt cgcggcgtac gtccgcgtct gggacttcgg cgcgggaccc gggtcgaacc
     3421 tggacttggc gagatcgagt gtccaggtgc cgacgaccga gtcggccgca ccggaagctc
     3481 cggccgcggc actcccgatg acgagcgcgg cacccacgac caacgaactg atgaacttga
     3541 gcatgtctgt cccctgtgag atgtatcttt cgcgcctggc catcctcccg cggacgcggt
     3601 ggccggccgg cagccgcgac tatgcgcgcc gggccccggc ccggcaatgc tgccggtcct
     3661 gtgacgtgca ccgcactcgc gacggccgaa cgagcccgtg cgccggcgca gacacgctcg
     3721 atgccaacag gcgcagggtg gcgttccagt acactgtcgc taccaccatc cccgccgccc
     3781 cgatcgcagg ctggcatgac acctgaacaa cgcggactga ggttccacat gcgcatgctg
     3841 atggcgcgga ccctgttgcc gttgctggcc acgttctcgt gcgccggcct gcaggcggcc
     3901 gagactggag cgcttgcagc cgactccgtc gacgacaccg acaacggatc cgtcagcgtg
     3961 atcgtgaagt ttcgcgacgc ccatcgtccg ggcgacgtgg cggacgcagc cgcggacgaa
     4021 gacgtcgccg cgctggcagg gcgaaccggc atcggacttg cgcacgtccg cagcatccag
     4081 ccgggcctgc gcgtcgtgaa ggtccgggca atcgctgccc cgggatcgct gggcgtggcg
     4141 ctgcaacggc tcagggagga tcccggcgtc cagtatgcgg agatcgacca gcggcggcat
     4201 gcgcatgcgg tcctgccgga cgacacgctc ttcgacgggc cgtactcggg acagtggtac
     4261 ctgaagaacg ccggcgacac cccgagcgcg atcgatgccg tgacggcctg ggacacgatc
     4321 cgcggcagca ccggcgtcat cgtcgcggtg ctggataccg gcgtccggta cgaccatccg
     4381 gacctgctgg cggcgggtga gggcggacgc ctgctgcccg gctacgactt catcagcagc
     4441 gcggccgagt cgaacgacgg cgatgggcgt gaccccgatc cgtcggaccc cggcgactgg
     4501 tcctcgagct cggacagttg cggagcgtcc ggcagttcgt ggcacggcac acgcgtgtcg
     4561 gggatcattg cggcgttgac caacgacacc atgggcgttg ccggcatcct gtggagcggg
     4621 tgggtcctgc cggtgcgagt cctgggcagg tgcggcggat tcgactcaga catcgtgccg
     4681 gccatgctct gggcggccgg aattcccgtt tccggaatgc catcgaaccc gtaccccgcg
     4741 cgtgtcatca acctcagcct cggcagcacc ggaagttgcc ccgcggaata cgccgatgcc
     4801 gtcaagcaat tgagtgatcg gggcgtgctg gtcgtggcct ccgccggtaa cgagggttcg
     4861 gttgtcgctg ttccggcgaa ctgcccgggt gtagctggca tcgccgcgat ccggcacatc
     4921 gggaccaagg taggattcag caacctggga cccgccgtcg cgctcagcgc tcccggaggc
     4981 aattgcgtca atgtcgaccc cggcctgccc tgcctgtact ccatcgacac gaccaccaat
     5041 ctcggcacga cgggtccagc ggccaacggc tacacggacc gattcgccga cgcgaatctc
     5101 gggaccagct tctcggcgcc gatcgtcacc ggcattgctg gcttgatggc agcagccaac
     5161 ggaaatctga cgtctgccca attgactgcc cggctgcgcg agggcgcgaa gccctttcct
     5221 gcctatcctg ccgacccgac cgaccccccg ctgccgacgt gtcacacgcc gactgacgcg
     5281 aacgacgttc aggaccagtg cgtgtgcacg gccgatacct gcggcgccgg catggcgaac
     5341 gcccgcggag cagtcgcggc agccctgcga ccgattgccg cgatcaccct gccgggccgg
     5401 ttctcgccgg gacaggacgt cgtcctgcga ggtggaagca gcggcgccgc atgcggtcat
     5461 gcgcttgcga gctatgcgtg gagcgtggtc gcagggacac ctccatcccc aatcgtcggc
     5521 gcgaacaccg acacggcgac cgtgaaggcg ccatcgtccg gctcctacac cgttcggctc
     5581 acggtggccg acgatgcggg gcgccaggac gtggcggaca tcgtcgtcca gccgacgctg
     5641 gcgacgaccg ctgcgccggc atcggcgggg gccacggcct gcccggcgcc gatcgttgcg
     5701 atcaccctgt cgccggtcct ggccacggtc caggcgcacg gcggaacgat cgctttctcg
     5761 gtgtcggtcg ccaatgcgac ggacaccggg gtcctctggt acgtcaatga cgcggcgggc
     5821 ggaaacgcga ccgttggaac gatctcgacg accgggttct atacggctcc agccagcgct
     5881 ccggcagccg ggagcgtcac gatcaaggcg gtgccagcgg cggatgcatc acgattcgcc
     5941 gccgcccgcg tgacgatcac ggcggcgccg gtcggtgccg gcggaggaag cggtggtagc
     6001 ggaggtggcg gtggcggcgg cacgttggac cccgtcgcac tgctcgcgct ccttgcctca
     6061 cgtgtcgcgc atctgagtcg ccgcccgcag cagcgaggga gcgaggcagc ccgctgaggc
     6121 ggacgccggg cctggcgatg ctggagcgaa tcttccgtct gcgcgagcac ggcacgtcgg
     6181 tccgcaccga actgacggcg ggcctgacca cgttcctgac gatgtcctac atcatcgtcg
     6241 tgcagcctgc cgtgctgtcg ggctcgctgt tcggcgagcc gaccggcatg gatttcggcg
     6301 cggtcacgac cgccacctgc ctcgcagccg cactggcttc ggcgatcatg ggactctacg
     6361 cgcgctatcc gattgcacag gcgccgggca tgggcgagaa cttcttcttc gtgttctccg
     6421 cgatcccggc ggcagcggct gcaggagcgg cggtcccttg ggcgacggcg ctgggcgcgg
     6481 tgttcgtgtc gggggtggtc tttctcgtgc tcacgctggc cggcgcgcgc gaagcggtga
     6541 tgaatgccct gagcccgagc atgaagcacg cgatcgctgc cgggatcggg ctcttcattg
     6601 ccttcatcgg cctgcagaat gccgaggtgg tggtcgggga ccccggcacc gctgtgacgc
     6661 tgaacccgga catcggttcg ccggacgtcg ccgtgttcct ggtcggactg ctcacggccg
     6721 ccggcttgca cgcccggcga atccggggct cgatcctgtg ggggattctc gccgcgggcg
     6781 cggtggccgt cggattgaag ctcgcactgc cgttgctcgg cgactccgtc atgggcgcgc
     6841 cgtcggtcgc caactcggcg ctggcgacgc gcttcgaact tgccagccac gtcgtcgccg
     6901 cgccaccgtc gctgcagcct acgttcctgc ggatggacgt gcgcggcgcc ctgacgctgg
     6961 cgatggcgcc ctatgtcgcg atcttcctgt tcatggtgtt cttcgacacc gtcgggacgc
     7021 tcgtcggcgt ggccgggcag gccgggctca tgccgaacgg ggtgctgcct cgcgcccggc
     7081 aggcgttcgt gtcggacgcc acggcgaccg tggtcggcgc gtgtctgggc acgagcacgg
     7141 tgacgagcta tatcgaaagc gccaccggcg tcgagcaggg cgggcgcacc ggcctcacgg
     7201 cgctcgccgt cgccgtgctg ttcctcgtcg cactgttctt cagccccgtg atcgcgatgc
     7261 tcggcgccta cccgccgatc acgtcgccgg cgctggtgct ggtcggcgcg atgatgctcg
     7321 gcaacatccg ccacctgcag tgggacgact atacggaggt gcttcccgca ttcctggtgc
     7381 tggcgggcat cccgttcacg tactccatcg ccgacggtct tgcgctgggg ctgatcgctc
     7441 atcccatggt caaggtactg gcgggccggc ggcgggagac acgcgcagcc tcgtggatca
     7501 tcgcggcatt gctcgccggc tatttcgtcg tcgtccgctc tgcagcctcg ggttgaaaca
     7561 cacatgaaga cgatcatcga accgttccgc atcaaggtcg tggaaccgat ccgcctcacc
     7621 acggtcgacg aacggcgcgc gctgctgcag cgggccggct ggaacctgtt cgccatacat
     7681 gcggacgacg tcatcatcga cctgctgacc gacagcggca cgagcgcgat gagcgccgac
     7741 cagtggggcg cggtgatgcg cggcgacgaa tcctacgccg ggtcgccgtc gtactaccgg
     7801 ttcgaggcgg cggtgcgcga actcatgaat ttccgccacg tgataccgac gcaccaggga
     7861 cgggcggccg aagcgatcct gttctccatc gtgggcgggg ccggccggat cgtcccttcc
     7921 aatacgcact tcgacacgac gcgcggcaac atcgaggcga ccggcgcacg ggccgtcgac
     7981 ctcgtcatcc acgaggcgca cgagccggac aacctgcacc cgttcaaggg caacatggac
     8041 gtggcgcggc tggaggacct gctgcgcgag tcgggtgacg cgatcccgct cgtgatgctg
     8101 acggtcacca acaacgccgg gggcgggcag ccggtgagcc tggagaacat ccgggccacg
     8161 gccgaggtcg cgcaccgcca cggcaagccg ctggtgatcg acggctgccg gttcgcggag
     8221 aatgcgtggt tcatcaagtg ccgcgaaccc ggccagggcg agcgtgccgt ggcggacatc
     8281 gtgcgcgaca tgttcgcggt cgccgacggc atgacgatga gcgcgaagaa ggacgcgttc
     8341 gccaacatcg gcggctggct cgcgaccaac gacgacgcgc tggcggccga ggcgcgcaac
     8401 cgcctgatcc tgaccgaggg gttcccgacg tacggcggcc tggccggccg ggacctcgag
     8461 gccatcgccg tcggcctgca cgagatcgtc gacgagcagt acctgcgcta ccgggtgcgc
     8521 acgaacgagt acatcggcga gaagctcacg gcgctcggcg ttccgatcgt gcggccggtc
     8581 ggcggtcatg cggtgttcgt cgacgcacga cgcttcctgc cccacattcc gccgctcgac
     8641 tatccgggcc aggccctcgc cgtcgcgttg tacgaggccg gcgggatccg tgcctgcgaa
     8701 atcgggaccg tgatgttcgg gatgggaccc gatggcgtgg agcgcgcggc ggacatggac
     8761 ctcgtccggc tcgccatgcc gaggcgcgtc tacacgcagt cgcacgcgga ttacctcgtc
     8821 gaggtgttcg aggaggtcgt gcggcggaag gccgaactgc gcggcttccg catcgtgtcg
     8881 cagccgccgc gcctgaggca cttcacggcg cgattccagc cactgggcta gagcggctcg
     8941 aagcgcgcct ggaagaagcg caggtacttc ggctcgtaaa ccattttcag gccgcgcgcc
     9001 gcgcctcgcg cgtcgtagac ggccttgacc gactcggcga cgacgtccat gtgtgcctgg
     9061 gtgtagacgc ggcgggggat cgtgaggcgc gtcagttcca gtttcgggta atggtgctcg
     9121 ccggtggcgg ggtcgcggcc cgcgctcacg ataccgcgct ccatcgagcg gatgccggag
     9181 tcgaggtaca gttccgcggc cagtgtctgg gacgggaact ggtcctgacg cagttgcggg
     9241 tagaacgccc gcgcgtcgag gaatatcgcg tgcccgccca cgggctggac catgggtacg
     9301 tcccagtcct gcagcagttc cgcgagatag cgcacctggc cgatgcgcgc ccgcatgtgg
     9361 tcgtcctgca tcgactcggc gatacctatc gccatggcct ccatgtcgcg cccggccagg
     9421 ccaccgtagg tgtgcaggcc ctcgtagacg accaccaggt tgcgcagttc ctcgaacagt
     9481 tcccagtcgc gtaccgccag ccatccgccg atgttcacga gcgcgtcttt cttcgcgctc
     9541 atccaggcac cgtccgtgtg gctgcagaac tccttcacga tctcggcgat gggcttgtcg
     9601 gcatagcccg gctcgcgctc ctggatgaag aacgcgttct ccgccaggcg cgtcgcgtcg
     9661 aggaacacgc gcacgccgtg cttcctgcag agtgcgtgca gggacctggc gttggccatg
     9721 ctgaccggct ggccgccggc catgttcacc gtcgcggcga ggctcacgta ggcgatgccc
     9781 cgtgcgccgt gccggtcgat cagcttctcg agtttctcga gatccacgtt tcccttgaac
     9841 gggctcgggt cggtgggatc gtgcgcggcg tcggcgatga cgtccacgaa gatgccgccg
     9901 gcgagctcct ggtgcaggcg cgtggtcgtg aagtacatgt tgccggggac gtactgaccc
     9961 ttgctgatcg cggcctgact cagcaggtgc tccgcgccgc ggccctggtg cgtgggcacg
    10021 acgtgctcgt agccgtacca gtgcctgatc gcctcctcga ggtggtagaa attgcggctg
    10081 ccggcgtagg cttcgtcgcc gagcatcatg cccgcccact ggcggtcgct catggcattg
    10141 gtgccgctgt cggtcaggag gtcgatgtag acgtcgtccg atcgcagcag gaacgtgttg
    10201 tagccggcat ccgcaagagc cctggcgcga tcttcgcgcc gagtcatgcg cagcggctcg
    10261 accatcttga tcttccaggg ctcggcccag gagcgtcgtc ctgactgctg gcccatggtc
    10321 cggtgcacgg tcttgctcat gacggttctc cgtgtctgcc gcgccggccc gacggctcac
    10381 gcaagtgcac cgcctgcgac gcagcggcga ctataggagc ggcgatatca ggccgacacc
    10441 gaggaaaacc gtggtgaccg ggtgacgagt gcggttcggg atggtgcccg gggccgggat
    10501 cgaaccggca tggggtcgcc cccgagggat tttaagtccc ttgcgtctac cagttccgcc
    10561 acccgggcag gcaaggcagg ccagatcata catccccggc ccgggtgtcg agcgcgatgc
    10621 ccgccacctc gtagccgcgg cggaagtcct ggacgtgccg ggtcatccag gcctgcgcct
    10681 cgtcggcgtc tccgcggcgc atggcttcga cgatgcagcg ctgcgcgacg gcgatgcgtg
    10741 aacgaccctg gggcacgcgg tcgatcacgc ggcgcagcga cggcgccagc agcttcgtca
    10801 cgggttgcat ggcgaggacc agcacgcggt tcgcgctggt cgcggcgagc gagccgaaga
    10861 attccacgac gccggccacg gcgccctcgg cgctgcgcgc ggtctccacc ttggcgatgc
    10921 agaattccag ccgctcgatg ttctcggggc ggggatgcgc cgccgcgagc cacgcggtgc
    10981 gcggcgcgat ggccagcatt gcctcccaca gctcgatgaa cgtcacgtcg tcgagcgcca
    11041 acgccctgct caggctcgac gtggtcgcgg cctgcccggg tcgcgtcacg cgcaggcgct
    11101 tggtacccgc ggcgcgcccg atgaggcccg ccgactcgag tcgtcggacg gcttcccgca
    11161 ccgtcgagcg gtgcacggag tactgctcgg ccagtgccgt ctcggacggc agcggatccc
    11221 cggggcggag cgaacggtcc aggatgcgcg cctcgaggtc cgcggcaatg cgcgcgaagg
    11281 cggcgctgcg cgtgccccgg tcggcgggct tgaggcgcgc cgttcgcccg gcaggacggg
    11341 cggggcgggc gcggtgcggg gcggcccgtg cgggtctcga acgcttgatt tgacggctca
    11401 taagattgtc cgacaatata cgcggcacga gcaggtggca agcgggcgga ggcgacgacg
    11461 gtggtggact tcgacttcgg gctcggcgag gatctcgacc tgctgcgcga gaccgtgcgg
    11521 gcattcgccg aacaggagct cgcgccgcgg gcggacgcga tcgaccgcac caaccagttt
    11581 ccccgcgacc tctggccgcg cctcggcgaa ctgggcctgc tcggcatcac ggtggagccg
    11641 gaatggggcg gcgccgggct cggctacctc gcccacgtgg tcgccatgga ggagatcagc
    11701 cgggcatccg ggtccgtcgg gctgtcgtac ggcgcccact caaacctctg cgtgaaccag
    11761 gtccggcgct ggggcagcca cgagcagaag gcgcgctatc tcccgaagct cgtgagcggc
    11821 gagcacctcg gcgcgctcgc catgagcgag accggagccg gctcggacgt catgggaatg
    11881 cgattgaggg cggaaccgca cggcgaccgc tacgtcctga acggcagcaa gatgtggatc
    11941 acgaacgggc cgaccgccga cgtgctcgtc gtctatgcga cggtcga
//
LOCUS       DAOMFD010000051        11829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1401_length_11829_cov_32.072872, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000051 DAOMFD010000000
VERSION     DAOMFD010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11829
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1401_length_11829_cov_32.072872"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="PL152_04690"
     CDS             complement(<1..981)
                     /locus_tag="PL152_04690"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HQR48609.1"
                     /translation="MSPVLPLAGIRVLTFENFGAGPYGSLYLADLGAEVVKVENREQG
                     GDATRGMGPFFLGEHDSHFFQTFNLNKKSVTLNLKLPEGQEAFRRLVRTADVVMNNLR
                     GDQPAKLGLDYASLASVNPRVVCAHLSAYGRDNERRGWPGYDYLMQAEAGYLHLTGEP
                     GTPPARMGLSIVDFMTGITTAVAVLAALIGVMKTGRGGDVDVSLFDVALHQLTYPGNW
                     YLNEGHRTERMPRSSHPSAVPVQLFRTADGWIFVMCMTEKFWQALTEAIRRPDLASDP
                     RFANVAGRREHRDELTRLLDEEFSGATTQRWLDRLQGVLPAAPVYDISQAL"
     gene            1188..3908
                     /gene="ppc"
                     /locus_tag="PL152_04695"
     CDS             1188..3908
                     /gene="ppc"
                     /locus_tag="PL152_04695"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQR48610.1"
                     /translation="MARKGIIFSTKDAALRDDVRVLGRIVGEIIRDQCGEEVFELVEG
                     DRKAAIGRRLGDADECVELVVRIQGRPVEQAREVIRAFTTWFQVVNTAEKVHRVRRRR
                     QYLTDSGTPQPGGLEECFRRLRDRGMTLAEVAALLDGVTLEPVFTAHPTESTRRTLLR
                     QQQRVARLLLEGMDPLRTPGERRATVERVRTELTTGWQTAVNSRERLTVADEREHVLF
                     FLVEVIYEVIPAFYEEMEAALVNAFGHEAEGVDLPTMLRFGSWVGGDMDGHPDVHAKT
                     IRESCNRHHRLIVNRYFLEAQALAEKLSQSENRIPVSDAINARIEAYRNLLPSARGSI
                     PVSHDRMPYRVFLGQVAERLRATYEGRSGQYDRVEQLLEDLEVIACSLEQHKGRNAGL
                     FYVRRMQRRARAFGFHLATLDVRQSADVHREIIGSALGDPEWATRAPAERAARLTEAL
                     SSDESPFEDLGPVGKRSLWVFEAIDFCRNRFGPGAVGSYVVSLARDADDVLSVLLLAR
                     WAGMAAGPGEQVPLDVTPLFESVAALEQADETLARLLSNPVYRAHVAARGNRQVVMIG
                     YSDSNKSAGILASRWVLRKAQEAMDAACERAGVRLQVFHGRGGAIGKGGGRNEALVRA
                     MPPSAVRGGLRVAEQGEAINDRYGLRPLALRSFEQVVSALTLVTAGEHGQEPVDARWR
                     EAMETLAGSSRTAYRSLVHDDPSFFDYFQAVTPVDVIERMQIGSRPVSRAADGGVAAL
                     RPLPWIFAWSQSRHMLPGWFGSGTGLAAAVERFGVGLLGDMYARWPFFEALLDDIEMM
                     LARADMGVARFYDQLARPEHGRYAEAITGEYAASVDRILAIKGETCLLDEEPTLQRSI
                     RLRNPYVDPLHLAQVDLLRRWRESGRQDRQLFEALVATVNGISQGLQGSG"
     gene            4015..4668
                     /locus_tag="PL152_04700"
     CDS             4015..4668
                     /locus_tag="PL152_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48611.1"
                     /translation="MAAKKKARRTTLRSSKGKKLYAVRDSKGRIRDIQTYKRAHAADL
                     RRKSKKETAAKKKAAKKKVTRTAAKAKGRLAKVREAIRKAMKKAPVRAKKQVTAARAA
                     VRKAPKQAQAAMRKAVKKLPPKAQKAVAKAEKQIKKTAAQAETRVATTVKAVKKQTAA
                     TVKQAEAAVAKAQDSAEKSAATVVEQAKDLFQKASDAVGTAVETVVEKVTPEQPASR"
     gene            complement(4732..5634)
                     /locus_tag="PL152_04705"
     CDS             complement(4732..5634)
                     /locus_tag="PL152_04705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48612.1"
                     /translation="MIHPAATLVLVREHGGALEALMMRRHSAIAFMGGMWVFPGGRMD
                     AADQSPEALSRILPGPHSPAPARLKSMQDQSLDPAAAVGLYIAACREAFEESGVLLAS
                     DSAGEPCAPDTASRLAPRREEVTADASAFVRMLRDEDLYIDLRRLVYWSHWITPSMEP
                     KRFDTRFFVAMLPHGQAASADHSELTEHVWIEPAAAVAAQARGELMFVAPTQLTLEDL
                     AESHARHGSAQAMLDAEGGRETPAVMPKIELAAERVRIVMPWDPGYASVAGEGCEPAA
                     AFPGHLARRPSVVTMARPSAPSRQ"
     gene            complement(5631..6641)
                     /locus_tag="PL152_04710"
     CDS             complement(5631..6641)
                     /locus_tag="PL152_04710"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HQR48613.1"
                     /translation="MTRTYQRIVLASRPTGPVTPDNFRLETVPVPEVADGQLLVRVHY
                     LSLDPYMKGRMLESRSYADPQPLNETMVGGVVGVVEATRNEKFAVGDTVVGRLGWQEY
                     GVSDGSGLVKIDASRIPMSAYLGVLGMPGVTAWYGLNRICEPKPGQTVVVSAASGAVG
                     SAVGQLAKMAGCRAVGIAGGPEKCSYVTSELGFDACIDHREHRDAKSMARAMRAAAPD
                     GVDCLFENVGGVCLDGTLACMNPHGRIALCGLIAGYEGEEIPIHNVRTLLVMRIRLQG
                     FIVSEHMDAWPPALKELAAGVIAGRIKYRETVAHGLASAPEAFIGMLHGRNFGKQVVK
                     IA"
     gene            6889..7953
                     /locus_tag="PL152_04715"
     CDS             6889..7953
                     /locus_tag="PL152_04715"
                     /inference="COORDINATES: protein motif:HMM:NF015400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HQR48614.1"
                     /translation="MAGRWLIYGANGYTGSRIARAARVQGQAPVLAGRNAAAVGALAA
                     ELGLPSSIFNLSDAAASRAALRDVDIVAHCAGPFSATSAPMADACIATRTHYVDITGE
                     IDVLVALQARDEEARRAGIALCPSMAFDIIPTDCIAAILKDALPDATHLTLGFAGLDE
                     LTAGTLKSALEGVGHGMSRVREGGRLVEIPYFSRRCRLDFGDGRGEVDGFNIPWGDVA
                     AAYWSTGIPNVEVYIPADAPMARSVRMMRPLRPLLQMRPVVRLLQRVVSATSKGPTDE
                     SLATTHTHVVGVARNALGLERRAQLQCPNGYRLSVDGVLMAVEHLVSHPGTFGFRTPS
                     MLMGTHCVERLPGVSPIVVK"
     gene            8145..8711
                     /locus_tag="PL152_04720"
     CDS             8145..8711
                     /locus_tag="PL152_04720"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQR48615.1"
                     /translation="MATIRGADFATLPNLLSLARIVGVAAAVLLYFAGHPLATVIIGT
                     AACLTDHLDGYLARRLGQETTLGAMLDQAADSFTTAIALAMLVIAGGFPFVFLIVFLA
                     REFWVGTVRRYAALGSREIPSHVSGKIATGFMYYSMFVMAVILMLGVPESISWWLHPL
                     AYYSMAFGLALSCYSGWRYTLVLAGKES"
     gene            8711..9859
                     /locus_tag="PL152_04725"
     CDS             8711..9859
                     /locus_tag="PL152_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR48616.1"
                     /translation="MPVVTARGRPEVLLCPGPVMLSPGVKAALAQCEVGHRDSRFSRV
                     VARLRQNCATVLGATADHSVMFVTGPATSGIEATFATLFPQERAVLVPVNGAFGLRLV
                     EILETHGIPCVPVHFGFGQPYALERIEAAIVEGSRAGVAALAMTHHETSAGIVNPVAG
                     VCAVARRHGLRTFVDATSSAGAEALDVGRDGVDACVTTSGKCLHGAPGVALVCVSREW
                     LGERCGSRPRTYSLDLRRYHDQLETNSQTPFTPAVPQFMALDRAVEELLQRGGVGRRG
                     LEYRRRRDFLAAAMGELGLSLLSLPRGTEASSILTVRVPEALGFDALYRAMQERGYVI
                     YGAKAPLAPDYFQLSVMGELSDADLSGFVESLRTVLARTAAEFQAASA"
     gene            complement(9891..10097)
                     /locus_tag="PL152_04730"
     CDS             complement(9891..10097)
                     /locus_tag="PL152_04730"
                     /inference="COORDINATES: protein motif:HMM:NF022832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit delta"
                     /protein_id="HQR48617.1"
                     /translation="MNVDRLVAMANEIAAFFSSEQDEAVAADQVANHLRRFWEPRMRR
                     AIVAHLQSGGTGLTPLARSGVARL"
     gene            complement(10094..>11829)
                     /gene="fdhF"
                     /locus_tag="PL152_04735"
     CDS             complement(10094..>11829)
                     /gene="fdhF"
                     /locus_tag="PL152_04735"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HQR48618.1"
                     /translation="YGLKSTLGESAGTQNFASVAHADVIVVIGANPTDGHPVFGSQMK
                     RRLRQGARLIVIDPRAIDLVRTPHVAADVHLQLRPGTNVAMINALAHVVVTQGLANDA
                     YAGERCEVESYRRWKAFVAEPRNSPEATEAVTGVPAALVRAAARLYAAGPNSAIYYGL
                     GVTEHSQGSTMVMGIANLAMATGHLGREGVGVSPLRGQNNVQGSCDMGSFPHEFSGYR
                     HVSDASTRALFEGAWQVPLASEPGLRIPNMFEAALDGSFRGLYIQGEDFVQSDPDTRH
                     VVAALEAMECVILQDLFLNETSKYAHVFLPGCSFLEKDGTFTNAERRISRVRKVMEPL
                     AGLADWEVTMRLAQALGHPMHYGHPSEIMQEIARLTPTFAGVTYEKLDRLGSIQWPCN
                     EAAPEGTPTMHVGSFVRGKGRFIVTEYVPTPERTTARFPLILTTGRTLFQYNVGAQTR
                     RTSNLTWQSEDRLDIHPHDAESRGIADGDWVGLASRTGETVMRATISRRMTPGVVYTT
                     FHFPESGANLVTTEHSDWATNCPEYKVTAVEVVKLEDPDAWRARRRAERDAARPARSG
                     AGATRDTVATS"
BASE COUNT         1824 a         4010 c         4160 g         1835 t
ORIGIN      
        1 gagcgcctgg gagatgtcgt agacgggtgc cgcggggagc acgccctgca ggcgatcgag
       61 ccatcgctgg gtcgtggcgc cggagaattc ctcgtccagc aggcgtgtca gttcgtcgcg
      121 gtgctcgcgg cggccggcca cgttcgcaaa tcgcgggtcc gatgcgaggt ccggacgccg
      181 gatggcctcg gtaagtgcct gccagaattt ctccgtcatg cacatgacga agatccaccc
      241 gtccgccgtg cggaacagct gcaccggtac cgcggacggg tgcgaactgc gcggcatgcg
      301 ttcggtacgg tgcccctcgt tcaggtacca gttgccgggg taggtgagct ggtgcagcgc
      361 gacgtcgaag agcgacacgt ccacgtcgcc gccgcgtccc gtcttcatca cgccgatgag
      421 cgcggcgagc accgccacgg cggtcgtgat cccggtcatg aagtcgacga tcgacagccc
      481 catgcgcgcg ggcggcgtgc ccggctcccc agtgaggtgc aggtagccgg cctccgcctg
      541 catcaggtag tcgtagcccg gccaaccccg acgctcgttg tcgcggccgt acgcggagag
      601 gtgcgcgcag acgacgcgcg ggttgacgga cgcgaggctg gcgtagtcga gcccgagctt
      661 cgccggctgg tccccgcgca ggttgttcat caccacgtcc gccgtgcgca cgagccgacg
      721 gaatgcctcc tggccctccg gcagcttgag gttgagcgtg acgctcttct tgttcaggtt
      781 gaacgtctgg aagaagtggc tgtcgtgctc ccccaggaag aacgggccca tgccgcgggt
      841 ggcgtccccg ccctgttcgc ggttctcgac cttgaccacc tcggcgccga ggtcggcgag
      901 gtacagcgag ccgtagggtc ccgcaccgaa attctcgaag gtcagcaccc ggattccggc
      961 caggggcagc acgggactca ctcgcgactc cgactcgaag gttggatccc cgcatgatac
     1021 cccgcaccgg cggcgtgacc gacatgggcg gccgggcggt gcgctcccac gcgccgtcga
     1081 ggtcggctct gctacgattc gcatcgacca tgaccattcg agccggcacc tgaccgggca
     1141 cccaccgacc cgcctccaag cagcccaccc gaatcgaccc ggcggccatg gcccgcaaag
     1201 gcatcatttt ttccaccaag gacgcggcac tgcgcgacga cgtccgcgtg ctcggccgga
     1261 tcgtcggcga gatcatcagg gaccagtgcg gcgaagaggt cttcgaactc gtcgagggcg
     1321 accgcaaggc cgcgatcggg cgccggctcg gcgacgccga cgagtgcgtc gaactcgtcg
     1381 tccgcatcca gggacggccg gtggaacagg cgcgcgaagt catccgggcg ttcaccacgt
     1441 ggttccaggt ggtgaacacc gcggaaaagg tgcatcgcgt gcgccgccgc cgccagtacc
     1501 tcaccgacag cggcaccccg cagcccggcg ggctcgagga gtgcttccgc cggctgcgcg
     1561 accgcggcat gacgctcgcg gaggtcgcgg cgctgctcga tggcgtgacg ctcgagccgg
     1621 tgttcaccgc ccacccgacc gagtccacgc ggcgcaccct gctgcggcaa cagcagcgcg
     1681 tcgcgcgcct gctgctcgaa ggcatggacc cgttgcggac ccctggcgag cggcgcgcca
     1741 ccgtggagcg cgtgcgcacc gagctcacga ccggctggca gacggcggtg aattcgcgcg
     1801 agcggctgac cgtggccgac gagcgcgagc acgtgctgtt cttcctcgtc gaggtgatct
     1861 acgaggtgat ccccgcgttc tacgaggaga tggaggccgc gctggtgaac gccttcggcc
     1921 acgaggccga gggcgtggac cttccgacca tgctgaggtt cgggtcgtgg gtcggtggcg
     1981 acatggacgg gcacccggac gtgcacgcga agacgatccg cgagagctgc aaccgccacc
     2041 atcgcctgat cgtcaaccgc tacttcctcg aggcgcaggc gctggccgag aagctgagcc
     2101 agagcgagaa ccgcatcccg gtgtcggacg cgatcaatgc gcgcatcgag gcctatcgca
     2161 acctgctgcc ttcggcgcgc ggttccatcc ccgtgagcca cgaccgcatg ccgtaccgcg
     2221 tgttcctcgg ccaggttgcc gagcggttgc gggcgacgta cgagggccgg tccggccagt
     2281 acgaccgggt cgagcagctg ctcgaggacc tggaggtcat cgcctgcagc ctcgagcagc
     2341 acaaggggcg caacgcaggg ctcttctacg tccgcaggat gcagcggcgg gcgcgggctt
     2401 tcggattcca cctcgcgacg ctcgacgtgc ggcagagcgc cgacgtgcat cgcgagatca
     2461 tcggcagcgc gctgggagac ccggagtggg cgactcgtgc gccggccgag cgcgccgcgc
     2521 gcctgaccga ggcgctgtcg agcgacgagt cgccgttcga ggacctggga ccggtcggca
     2581 agcgctcgct gtgggtgttc gaggcgatcg atttctgccg caaccgtttc gggccgggtg
     2641 ccgtgggttc ctacgtcgtg agcctcgcgc gcgatgcgga cgacgtgctg tcggtgctgc
     2701 tgctcgcgcg ctgggcgggc atggccgcgg gcccgggcga gcaggtgccg ctggacgtga
     2761 ccccgctctt cgagtcggtc gcggcgctcg agcaggccga cgagaccctc gcgcggctgc
     2821 tgtccaaccc ggtctaccgg gcgcacgtcg ccgcccgcgg caaccggcag gtcgtgatga
     2881 tcggctactc ggacagcaac aagtccgcgg gcatcctggc ctcgcggtgg gtgctgcgaa
     2941 aggcgcagga ggcgatggat gccgcctgcg agcgagccgg cgtgcgactg caggtgtttc
     3001 acggtcgcgg cggcgcgatc ggcaagggtg gcggccgcaa cgaggcgctg gtgcgggcga
     3061 tgccgccgtc cgcggtccgc ggcggcctgc gggtggcgga gcagggcgaa gccatcaacg
     3121 atcgctatgg gctccggccg ctggcactgc gcagcttcga gcaggtcgtc agcgcgttga
     3181 cgctggtgac cgcgggcgaa cacggccagg agccggtcga cgcccggtgg cgcgaggcca
     3241 tggagaccct ggccgggtcg agccggaccg cttaccggtc cctggtgcac gacgatccgt
     3301 cgttcttcga ctatttccag gcggtgaccc cggtggacgt catcgagcgc atgcagatcg
     3361 ggtcgcgccc ggtgtcccgc gctgcggatg gtggcgtcgc ggcgctgcga cccttgccct
     3421 ggatctttgc ctggtcgcag agccgccaca tgctccccgg ctggttcggg tcgggaaccg
     3481 ggctggccgc cgccgtggag cggttcggcg tgggcctgct gggcgacatg tacgcccgct
     3541 ggccgttctt cgaggcgctg ctcgacgaca tcgagatgat gctggcgcgc gccgacatgg
     3601 gcgtggcgcg cttctacgac cagctggcgc ggcccgagca cggccgctat gcggaggcga
     3661 tcaccgggga gtacgccgcc agcgttgacc ggatcctggc gatcaagggc gagacctgcc
     3721 tgctcgacga ggaaccgacg ttgcagcgct ccatccggct gcgcaaccct tacgtggatc
     3781 cgctgcacct ggcgcaagtg gacctcctga ggcgctggcg cgagtccggc cgacaggacc
     3841 gccagctgtt cgaggctctg gtcgcgacgg tgaacggcat ctcccaggga ctgcagggct
     3901 cgggctgact gcagtttccc ttgttgactc ccgtctggcg ctgctctagc gtcagaagcg
     3961 aggcatctca tcgcgcggcg atcgccgcgt caccggacac acgaggggtt ctccatggca
     4021 gcgaagaaga aggcgcggcg caccacattg cgcagcagca agggcaagaa actctacgcg
     4081 gtgcgtgatt ccaagggccg gatccgcgac atccagacct acaagcgtgc gcacgcagcc
     4141 gacctgcgtc gcaagtcgaa gaaggaaacc gccgcgaaga agaaggcggc caagaagaag
     4201 gttacgcgca cggccgccaa ggccaagggc cggctcgcca aggtgcggga agcgatccgc
     4261 aaggcgatga agaaggcgcc ggtgagagcg aagaagcagg tcactgcggc gcgcgccgcc
     4321 gtgcgcaagg cgccgaagca ggcgcaggcc gcgatgcgga aggcggtgaa gaagctgccg
     4381 ccgaaggcgc agaaggcagt ggccaaggcc gagaagcaga tcaagaagac ggcggcccag
     4441 gccgagacca gggttgcgac gaccgtgaag gccgtgaaga agcagacggc cgcgaccgtg
     4501 aagcaagcgg aggcagcggt tgccaaggcc caggactctg cggagaagtc ggctgcgacg
     4561 gtcgtcgagc aggcgaagga cctgttccag aaggccagcg acgcggtcgg gacggccgtc
     4621 gagaccgtag tcgagaaagt cacgccggag cagccagcgt cgagatagcg ccggcatcac
     4681 tgtccgcctg acgaacgagg ccccgcggcc gcaaggccgc ggggcttttt gtcactgccg
     4741 tgacggcgcc gacgggcgcg ccatcgtcac cacggagggt cgcctcgcga ggtgcccggg
     4801 gaatgcggcc gcaggctcgc agccttcgcc cgcgaccgac gcgtagcccg ggtcccacgg
     4861 catgacgatg cggacccgtt ccgcggcgag ttcgatcttc ggcatcacgg ccggggtctc
     4921 gcgtccgccc tcggcgtcga gcatcgcctg cgccgagccg tgacgcgcat gcgactccgc
     4981 gagatcctcc agcgtgagct gtgtcggggc cacgaacatg agctcgccgc gcgcttgcgc
     5041 ggcgaccgcc gccgctggtt cgatccagac gtgctccgtg agttcggaat ggtcggcgct
     5101 cgccgcctgg ccatgcggca gcatggccac gaagaagcgc gtgtcgaagc gcttcggctc
     5161 catggaaggc gtgatccagt gcgaccagta cacgagccgg cgaaggtcga tgtacaagtc
     5221 ctcgtcccgc agcatccgca cgaacgccga cgcgtcggca gtcacctcct cgcgccgcgg
     5281 cgcgaggcgc gacgcagtgt cgggagcgca gggctcgcct gccgaatcgc tggcaagcag
     5341 gacaccactt tcctcgaaag cttcgcggca ggccgcgatg tacaggccca ccgcggcagc
     5401 cgggtccagc gactggtcct gcatggactt caagcgggcg ggcgcgggtg agtgtggtcc
     5461 cggaaggatg cgcgagagtg cctcgggcga ctggtccgcc gcatccatgc gcccgccggg
     5521 aaacacccac atgccgccca tgaatgcgat cgcggaatgc cggcgcatca tcagcgcctc
     5581 gagtgcacca ccgtgctcgc gcaccaggac cagcgtcgcg gccgggtgga tcatgcgatc
     5641 ttcacgacct gcttgccgaa gttgcggccg tgcagcatgc cgatgaatgc ctccggggcg
     5701 gacgcgaggc cgtgtgccac ggtttcccgg tacttgatgc gacccgcgat cacgccggcg
     5761 gccagttcct tgagtgccgg cggccacgcg tccatgtgct cgctcacgat gaatccctgc
     5821 aggcggatgc gcatgaccag cagcgtgcgc acgttgtgga tcgggatctc ctcgccctcg
     5881 tagccggcga tcaggccgca cagggcgatg cgcccatgcg gattcatgca ggccagcgtg
     5941 ccgtcgaggc agaccccacc cacgttctcg aacaggcagt ccacgccgtc cggcgcagcg
     6001 gcacgcattg cacgggccat cgacttcgcg tcgcggtgct cgcggtggtc tatgcaggcg
     6061 tcgaagccga gctccgaggt gacgtaggag cacttctcgg ggccgccggc gattccgacc
     6121 gcgcggcagc cggccatctt ggcgagctgg ccgaccgcgc tgccgaccgc gccactggcg
     6181 gccgacacga ctacggtctg gcccggcttc ggctcacaga tgcggttcag tccgtaccac
     6241 gccgtcacgc ccggcatgcc gagcacacct agatacgccg acatcgggat gcggctcgca
     6301 tcgatcttga cgaggccact accgtcggag acgccgtatt cctgccagcc gagtcgcccc
     6361 acgaccgtgt ccccgaccgc gaatttctcg ttgcgagtcg cctcgacgac gccgaccacg
     6421 ccaccgacca tcgtctcgtt caggggctgc gggtccgcgt acgaacggga ttcgagcatg
     6481 cggcccttca tgtacgggtc gagcgacagg tagtggacgc ggaccaggag ctggccgtcg
     6541 gcgacctccg gcacgggcac ggtctcgaga cgaaagttgt caggagtgac ggggcccgtc
     6601 ggccggctgg cgagcacgat gcgctgatag gtcctggtca tggaatgtct ccggtgatcg
     6661 cacgacagag cgcgacactt tgcacgattg cccggataac accaaaggct cgcgagcgcg
     6721 ctgcagacgt cgcgtccgtc ggtgacttcg gggcaccgtt cgggatccgg aaatgacctt
     6781 caaaaacaac gaggtgcgcc gcgcgccgcg aatcgttcgc agaagccggc gtcgcgtcgg
     6841 acccggtggc cgggtaggct gcgcgcgagg cagtcctcgg ggggtgacat ggcgggtcgc
     6901 tggctgatct acggggcgaa tggctacacc ggcagtcgca tcgcccgtgc cgcccgcgtg
     6961 caaggccagg cgccggttct cgccgggcgc aatgccgcag ccgtcggcgc actggcggcg
     7021 gaactcggat tgccctcgag catcttcaac ttgagcgacg cggctgcgtc ccgcgccgca
     7081 ctccgtgacg tggacatcgt tgcccattgc gccggtccgt tctcggccac cagcgccccc
     7141 atggcggatg cctgcatcgc cacacgcacc cactacgtgg acatcaccgg cgaaatcgac
     7201 gtgctggtcg cactgcaggc gcgcgacgag gaagcccgcc gcgccgggat cgcgctctgc
     7261 ccgagcatgg ccttcgacat catcccgacc gattgcatcg cggccatcct gaaggacgcc
     7321 ttgcccgacg cgacgcacct cacgctcggt ttcgcgggcc tcgacgaact gacggccggg
     7381 acgctgaagt cggccctcga gggcgtcggc catggcatgt cgcgcgtgcg cgagggcggc
     7441 cgcctggtcg agatcccgta tttttcccga cgatgccggc tggatttcgg cgacggcagg
     7501 ggcgaggtgg atggcttcaa catcccgtgg ggcgacgtcg cggccgcgta ctggtcgacc
     7561 gggatcccga acgtcgaggt ctacattcct gccgacgcgc ccatggcgcg cagcgtccgc
     7621 atgatgcggc cgctgcgacc attgctgcag atgcgcccgg tggtgcgact gctgcagcgc
     7681 gtggtgtccg cgaccagcaa gggaccgacg gacgaatcgc tcgcgacgac tcatacgcac
     7741 gtcgtcggag tcgcgcggaa cgccctcgga ctggaacgcc gggcgcagct gcagtgtccc
     7801 aacgggtatc gcctgagcgt ggatggagtg ctcatggcgg tggagcacct cgtgtcccat
     7861 ccgggaactt tcggcttccg cacgccctcc atgctgatgg gcacccactg cgtggagcgc
     7921 ctgccgggcg tctcgcccat cgtcgtgaag tgacggcgcg ccaccgcaca atcgggaaag
     7981 cccttacttg cgcgcgacca gcggtggatc caccgcacgc tggggcggag ccaaggtccg
     8041 agccgatgcg ttagtatcga acgatcgagc agcgcgtccg accacttcga cggaaccacg
     8101 aaacgccaat gacgccgggc actgcaactc tccaggcgaa gggaatggcg accatccgcg
     8161 gcgcggactt cgcgaccctg cccaacctgc tgtcgctggc acggatcgtc ggtgtggccg
     8221 ccgccgtcct gctgtacttc gccggtcatc cgcttgcgac cgtcatcatc ggcacggccg
     8281 cgtgcctcac ggaccacctg gatggatacc tcgcgcgccg gctggggcag gagacgacgc
     8341 tcggggcgat gctcgaccag gcggccgaca gcttcaccac ggccattgcg ctcgcgatgc
     8401 tggtgatcgc cggcggattt ccgttcgtgt tcctgatcgt gttcctggcc cgggaattct
     8461 gggtgggaac ggtccgccgc tacgcggcgc tcggcagccg tgagatcccg tcgcacgtgt
     8521 ccggcaagat cgccacggga ttcatgtact actcgatgtt cgtcatggcc gtgatcctga
     8581 tgctcggcgt gccggagtcc atctcctggt ggctgcaccc gctcgcgtac tacagcatgg
     8641 ccttcggcct ggcgctcagc tgctattcgg gctggcgcta caccctcgtg ctcgctggaa
     8701 aggagtcctg atgcccgtcg tcaccgcgcg cggccggccc gaagtcctgc tctgtcccgg
     8761 gcccgtcatg ctgtcgccgg gcgtgaaggc cgcgctcgcc cagtgcgaag tggggcaccg
     8821 cgattcgcgc ttctcgcggg tagtcgcgcg cctgcgccag aactgcgcga cggtgctggg
     8881 tgcgacggcc gaccacagcg tgatgttcgt caccggcccg gcgacgtccg gcatcgaggc
     8941 gacgttcgcc acgctgtttc cgcaggagcg cgcggtgctg gtcccggtga acggggcgtt
     9001 cggattgcgc ctggtcgaga tcctcgagac ccacggcatc ccctgcgtgc cggtccattt
     9061 cggcttcggg cagccgtacg cgctcgagcg catcgaggcc gcgatcgtcg agggcagtcg
     9121 agccggcgtc gcggctctcg ccatgactca ccatgaaacg tcggcgggca tcgtcaatcc
     9181 ggttgcgggg gtttgcgcgg tcgcacgacg acacggactg cgcacgttcg tggacgcgac
     9241 ctccagcgcc ggtgccgagg cgctcgacgt cggacgcgac ggcgtggacg catgcgtgac
     9301 gacctccggc aagtgcctgc atggcgcgcc cggggtggcg ctcgtctgcg tgagccgcga
     9361 gtggctcggc gagcgctgcg ggtcgcggcc gcggacgtat tcgctcgacc tgcggcgcta
     9421 tcacgaccag ctcgagacga attcccagac gccattcacg ccggcggtgc cgcagttcat
     9481 ggcgctcgac cgtgcggtcg aggaactgct gcagcgaggc ggagtcggca ggcgcggcct
     9541 cgaataccgt cgtcgccgcg attttctcgc cgcggcgatg ggagagctgg gcctgtccct
     9601 gctgtcgctg ccgcgcggga cggaggcgag ctcgatcctg accgtgcggg tccccgaggc
     9661 gctgggattc gacgcgctgt accgggcgat gcaggagcgc ggctacgtga tctatggcgc
     9721 gaaggcgccg ctcgcgcccg actacttcca gctgagcgtg atgggcgaat tgtccgacgc
     9781 cgacctgtcc gggttcgtgg aatcgttgcg cacggtgttg gcgcggacgg ctgcggagtt
     9841 tcaggccgcc tcggcctgaa gccgcgggct cgccagtaca cgccgatacc ctacaggcgc
     9901 gcgacacccg atctcgcgag gggcgtgagt cccgtcccac ctgactgcag gtgggcgacg
     9961 atggcgcggc gcatgcgcgg ctcccagaac ctgcgcaggt gattggcgac ctgatcggcc
    10021 gccacggcct catcctgttc cgatgagaag aacgccgcga tctcgttcgc catcgcgacc
    10081 agccggtcga cgttcatgac gtggcgaccg tgtcacgcgt ggcgcctgcg ccggaccggg
    10141 cagggcgcgc tgcgtcgcgc tcggcacgtc tccgtgcccg ccacgcgtcc gggtcctcga
    10201 gcttgacgac ctcgactgca gtgaccttgt attccgggca gttggtcgcc cagtcggagt
    10261 gctctgtcgt cacgagattc gcgccagact cggggaaatg gaatgtcgtg tacaccaccc
    10321 cgggcgtcat gcgcctggag atcgtcgcgc gcatcacggt ctcgccggtc ctgctcgcga
    10381 ggcccaccca gtcgccgtcg gcgatgccgc ggctctcggc gtcgtgcgga tggatgtcga
    10441 gccggtcctc ggactgccag gtgaggttcg acgtgcggcg ggtctgcgct cccacgttgt
    10501 actggaacag cgtgcgcccc gtggtgagga tgagcgggaa gcgggcggtc gtccgctccg
    10561 gtgtcggcac gtactcggtg acgatgaacc tgcccttgcc gcgcacgaac gatccgacgt
    10621 gcatggtcgg cgtgccttcc ggcgcggcct cgttgcacgg ccactggatg ctgccgagac
    10681 ggtcgagctt ctcgtaggtg acgccggcga acgtcggcgt gaggcgcgcg atctcctgca
    10741 tgatctcgga cgggtggccg tagtgcatcg ggtgccccag ggcctgcgcg aggcgcatcg
    10801 tcacctccca gtccgccagc cccgcgagcg gctccatgac cttgcggacg cgtgagatgc
    10861 ggcgttcggc gttggtgaac gtgccgtctt tctcgaggaa ggagcagccg gggagaaaga
    10921 cgtgcgcgta cttcgacgtc tcgttcagga acaggtcctg caggatcacg cactccatcg
    10981 cctcgagcgc cgccacgacg tggcgcgtat ccgggtcgga ctggacgaag tcctcgccct
    11041 ggatgtacag gccgcggaag ctgccgtcga gggccgcctc gaacatgttg gggatgcgca
    11101 gcccgggctc ggatgcgagc ggcacctgcc acgcgccctc gaacaaggca cgcgtcgacg
    11161 cgtccgaaac gtgtcggtag ccgctgaact cgtgcgggaa cgagcccatg tcgcacgacc
    11221 cctgcacgtt gttctgcccg cgcagcgggc tcacgcccac gccctcgcgt ccgaggtgac
    11281 ccgtggccat cgcgaggttc gcgatcccca tcaccatggt cgagccctga ctatgctccg
    11341 ttactccaag tccatagtag atcgcgctgt tggggccggc cgcatagagg cgcgccgcgg
    11401 cgcgcacgag tgccgcgggc acaccggtca cggcctcggt ggcctccgga gaatttcgcg
    11461 gttcggcgac gaacgccttc cagcgccggt acgactcgac ctcgcagcgt tcccccgcat
    11521 aggcgtcgtt cgcgagccct tgggtgacca cgacgtgggc gagggcattg atcatggcca
    11581 cgttggtgcc cggccgcagc tgcaggtgca cgtcggccgc aacgtgtggc gtgcgcacga
    11641 ggtcgatcgc ccgcggatcg atcacgatca gcctggcacc ctggcgcagt cgccgcttca
    11701 tctgcgaccc gaacaccgga tggccgtcgg tcggattggc gccaatcacc acgatcacgt
    11761 cggcgtgcgc tacggatgcg aaattctgcg tgcccgccga ttccccgagc gtgctcttga
    11821 gcccgtagc
//
LOCUS       DAOMFD010000052        11712 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1427_length_11712_cov_34.525092, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000052 DAOMFD010000000
VERSION     DAOMFD010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11712)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11712)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11712
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1427_length_11712_cov_34.525092"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /gene="dnaN"
                     /locus_tag="PL152_04740"
     CDS             <1..507
                     /gene="dnaN"
                     /locus_tag="PL152_04740"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HQR48619.1"
                     /translation="GVLELQRILGAEDRDLEVAIGTNHVRVQIGDIRFTSKLIDGRFP
                     EYGRVIPSNPGLIVTASRDELRQALQRAAILSNEKYRGIRFAVKPNTLVIQSHNPEQE
                     EAEEQLEVAYDGAELDIGFNVNYLLDALGAIEGAEVEVGLTDGNSSCLIRSNVDAGTR
                     FVVMPMRL"
     gene            526..1599
                     /gene="recF"
                     /locus_tag="PL152_04745"
     CDS             526..1599
                     /gene="recF"
                     /locus_tag="PL152_04745"
                     /inference="COORDINATES: protein motif:HMM:NF000819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HQR48620.1"
                     /translation="MSLERLAIEDFRCVERAELEFDPRCNLITGENASGKTSLLEAIF
                     FLGRGRSFRSNRNELLIRSGAERFNLYARILRHGVNQPLGMRVAGDGIEARYAGRPVA
                     GLAELATLMPVQAIDPEVHRLVEGGPQERRRYLDWGVFHVEPGFVEHWRRYQRALRQR
                     NASLRAGQAEALVRAWDEELIAAGEEVAAFRRRYVAGLQGALRAAGQSLLGADVDISL
                     HQGWSAERSLAQSLEQSLPRDRERGLTHIGPHRADLAIRMDGQLARDRVSRGQQKLLA
                     SAMLLGQLAYDAERGSGVAALLVDDPAAELDAGSLDRLLGQVLKLPAQFFVTALDPRN
                     ACLERLPAGHRFHVEHGLVTRLI"
     gene            1628..4036
                     /gene="gyrB"
                     /locus_tag="PL152_04750"
     CDS             1628..4036
                     /gene="gyrB"
                     /locus_tag="PL152_04750"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HQR48621.1"
                     /translation="MSGADVYDSSKIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFE
                     VVDNSIDEALAGYCTDVSVTIHEDQSVTVTDNGRGIPVDIHPEEGRSAAEVILTVLHS
                     GGKFDDSSYKVSGGLHGVGVSVVNALSETLLLTIYRDGQEYRQEYRLGEPVAPLAAFG
                     PSDRRGTTIRFKPSDTIFSNIEFEYEILQKRLRELSFLNSGVRIELVDERDDRREMFH
                     HEGGIREFVRYLNRSRTAIHESIFWFRGERDGIGVELALQWNDSYAETMSCYTNNIPQ
                     RDGGTHLAGFRAALTKTLNDYIESELASKRDRVPTTGDDSREGLTAVLSVKLPDPKFS
                     SQTKDKLVSSEVKGVVESIVAEKLSEFLLENPAQAKAVVNKIIDAARAREAARKAREL
                     TRRKGALDIAGLPGKLADCQEKDPALSELFIVEGDSAGGSAKQGRDRRTQAILPLKGK
                     ILNVERARFDKMLSSAEVGTLITALGCGIGKDDFDIAKLRYHRIIVMTDADVDGSHIR
                     TLLLTFFYRQIPLLIERGHVFIAQPPLYKVKKGKSETYVKDDGELNRMLLRSALEDAE
                     LHVNAGAEAMSAGALETLARRYMEFQAAAKRSPRRYDLHVLEQMLQLPELSAADREDA
                     AKLSAWGAQLETMLNVAQPAGRKYTVLVHQGTPPHLVVSRTEHSVTVEKHLHREFFSS
                     SDYRRIAELSRTLAGLFGPGAHVKKGEAMQEVTSFREALLWMLEQARKGQSIQRYKGL
                     GEMNPDQLWDTTINPETRRLVQVKIEDVLGADEIFSTLMGDQVEPRREFIERNALAAT
                     NIDV"
     gene            complement(4204..>4881)
                     /locus_tag="PL152_04755"
     CDS             complement(4204..>4881)
                     /locus_tag="PL152_04755"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQR48622.1"
                     /translation="AGAQAPYQPSVDGTTLPAIPLEAIRDGLQNRVPVVIGTNADETG
                     LEVPQTFSDADYAAFLNSTVQDPAIRAQVASLYSAANFGTSRAAYVALTSDVKFTCPA
                     RSIARALTASQSEPVFRYFFTEVPDGPNAPLFGSFHALELFYLFGVMNIRGYVPTAAE
                     TALSSNMQHYWGGFAATGAPAASGAPAWTQYDPARDNYLRLDSTALGMADGVNTARCD
                     FWSSLGI"
     assembly_gap    4882..4981
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4982..5641)
                     /locus_tag="PL152_04760"
     CDS             complement(<4982..5641)
                     /locus_tag="PL152_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HQR48623.1"
                     /translation="MRAFRTTMLALLLSACGGGGGSGSGSGGAPPPVGPADGLATTSC
                     GSYVGTDRGGSWSFLGIRYAAAPVGSLRWRSPAAPPCPAGNVPAGTYAQACPQFDRTT
                     GSPVGNEDCLTVNVFAPKSSFPSGLAPVMVFIHGGGNSMGSASEVIDGNKRLYDGKAL
                     AEATGNVVVTLQYRLGPLGWLVLPGLDAEAPDARSGNYGLEDMVAALKWVQRDIGAFG
                     GD"
     gene            5842..7110
                     /locus_tag="PL152_04765"
     CDS             5842..7110
                     /locus_tag="PL152_04765"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HQR48624.1"
                     /translation="MDRLDTDARQDFRPHEHAMEAPARSPRPSMSGRLVRSVLTRLGD
                     PRIEFELWTGERIRTSLEPPLGRLIIRDVRTLREICMRGEVGMGDAYADGGLEVDGDL
                     ASILEDVYLHSPRRADDTGRPAAATRRSRRAHRNTLVGSRDNIHHHYDIGNAFYSLWL
                     GETMSYTCAYYPTPDATLDAAQVAKMDHVCRKLRLQPSEHVLEAGFGWGTLATHMASR
                     YGVRVRAYNISEEQVRFARERAAGLGLGRQVEYVHDDYRNATGPCDAFVSVGMLEHVG
                     TENYATLGHVISRCLKPNGRGLVHSIGRNQPEPLHPWIERRIFPGAYPPSLKEMMDIF
                     EPWNLSVLDVENLRLHYARTCRHWLEAFEAARDRVAAMFDERFVRMWRLYLAGSTAAF
                     RTGELQLFQVVFAPGRSNDVPWTRADLYAR"
     gene            7126..8247
                     /locus_tag="PL152_04770"
     CDS             7126..8247
                     /locus_tag="PL152_04770"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HQR48625.1"
                     /translation="MQSCEALIVGGGPAGSTCARALTRFGLDVVVIDKAGFPRDKVCA
                     GWVTPPVLETLEIDVDDYRNGRVLQDITGFRTGVIGGGSATTRYDGPVSFGIRRCEFD
                     DFLLRRCGARLRLGETVTTIERVADGWVVNGTLSTRILVGAGGHFCPVARLLGTNVGR
                     AESTVVAQEMEFELDGAGPGDVEAGQPELYFCRDLSGYGWSFRKGTYLNVGLGRENGH
                     GMSEAVREFWKWLEAQGKVPAGMAPRMKGHAYVLYGHASRPRVADRVVAIGDAAGLAY
                     AQSGEGIRTAVESGVLAARAIVAAKGDYSRAALSRYEAAIEERFGRAAPSAASGQPPG
                     RLRAAAGRWLLSRPWFVRRVVLDRWFLHAHVPALEVRAV"
     gene            8415..8537
                     /locus_tag="PL152_04775"
     CDS             8415..8537
                     /locus_tag="PL152_04775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48626.1"
                     /translation="MFTRHSTDRLLSEVLAGLAITVAAVFAAMTYAVVHAQSFA"
     gene            complement(8544..9182)
                     /locus_tag="PL152_04780"
     CDS             complement(8544..9182)
                     /locus_tag="PL152_04780"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043231 - intracellular membrane-bounded
                     organelle [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDGSH iron-sulfur domain-containing protein"
                     /protein_id="HQR48627.1"
                     /translation="MTSSKESVRGRDVTIHFEARKCIHSRHCVLDRPDVFVPNVAGEW
                     IHPDNATPDEVAALAANCPSGAITYERHDAGPNERPPLVNTVRVRENGPLGFHADMKI
                     GDATALRATLCRCGASAHKPYCDGSHGRIGFVASGEPATTESAPLVNRDGTLEIAPQA
                     NGPLKVTGSLEIVSGTGRTIKRTTEVYLCRCGASANKPFCDGSHRKAGFRTD"
     gene            complement(9260..9994)
                     /locus_tag="PL152_04785"
     CDS             complement(9260..9994)
                     /locus_tag="PL152_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR48628.1"
                     /translation="MQYLRSLLYTILFFAVTLLFGFAVLIAAVLPLSLEQRYVIPRTW
                     GLFLTWLAGAICGLHYVVEGRENLPDRPFISLWKHSSSWDTLAQMFVVPPASWLLKRE
                     VTWIPVVGWAVKTYKPIAINRSAGHSAVNQVIAQGRERLAAGMGIIVYPEGTRVAAGQ
                     TRKYGISGALLGTQTGAPVVPIAHNSGYFWGRRSILKSPGTIRVVIGPPIDPLGLDPR
                     EINARAQQWIEGKIAEIVAQPGGKPD"
     gene            complement(10001..>10523)
                     /locus_tag="PL152_04790"
     CDS             complement(10001..>10523)
                     /locus_tag="PL152_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HQR48629.1"
                     /translation="RDVYDYVFERLRAYYLDGEAGMTVTTEMFDAVLATRPASPLDFD
                     SRLRALARFLGLPDAASLAAANKRIANILRKAPGPIGEAIDDSLLRDPAEQVLAEQVL
                     AVARLVEPKFRAREYTEALQSLAVLRKAVDEFFDSVMVMAEDPALRGNRLALLGRLRA
                     LFMHAADLSRLPG"
     assembly_gap    10524..10623
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10624..11648)
                     /gene="glyS"
                     /locus_tag="PL152_04795"
     CDS             complement(<10624..11648)
                     /gene="glyS"
                     /locus_tag="PL152_04795"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:NF014183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HQR48630.1"
                     /translation="MNPAARDFLVEIGTEELPPKSLLTLASAFADGIASGLDTAGVKH
                     GAVERYATPRRLAVRVRRLAEQQPDRNIERRGPPLKAAFDAQGSPTQAALAFARSCGT
                     EVAALEKLETPKGVWLVHRGTEPGARTSVLLPGIVQSALDALPIAKRMRWGAGTAEFV
                     RPVHWVLMLFGRDEIPCEILGVPAGGVTYGHRFMAPKALRVGTPSAYVAVLRKRGRVM
                     ADIHERRETIRQGVAAVASRLQGEAVIDESLLDEVTALVEWPVPLAGRFDAQFLELPA
                     EVPIATMQDHQRYFPVRDSSGKLMPWFVTVANIESADPEQVIAGNERVVRPRLSDAAF
                     FWTTDRKS"
BASE COUNT         1917 a         3780 c         3973 g         1842 t
ORIGIN      
        1 ggcgtcctcg agttgcagcg gattctcggg gcggaggacc gggatctgga ggttgcgatc
       61 ggtactaatc acgttcgcgt ccagatcggc gacattcgct tcacttcgaa gcttatcgac
      121 ggccgttttc cggagtatgg ccgcgtgata cccagcaacc cgggcctgat cgtcacagcg
      181 agccgtgacg aactccgcca ggcgctgcag cgagcggcaa tcctttcgaa cgagaagtat
      241 cgcggtatcc gttttgcggt gaagccgaat accctggtca tccagtcgca caaccctgaa
      301 caggaagagg ccgaggagca gctcgaggtc gcttacgacg gagcggagct ggacatcggc
      361 ttcaacgtca actacctgct cgacgcgctt ggagcgatcg agggagcgga agtcgaggtc
      421 ggtctgaccg acggcaacag cagctgcctg atccggtcga atgtcgatgc ggggacgcgc
      481 ttcgtcgtca tgccgatgcg gctctgaacc atcgggcgcg ggccgatgag cctcgagcgg
      541 ctcgcgatcg aggacttccg atgcgtcgag cgagctgagc tcgagttcga cccgcgctgc
      601 aacctgatca ccggggaaaa cgcctcgggc aagaccagct tgctcgaggc gattttcttt
      661 ctggggcggg ggcgctcgtt tcgatcgaat cgcaacgagt tgctgatccg ctcgggtgcg
      721 gaacgattca acctgtatgc gcgaatcctg cgccatggcg tgaaccagcc gctcggcatg
      781 cgcgtcgcag gggacggcat cgaggcccgc tatgcgggcc gaccggtcgc cgggctcgca
      841 gagttggcga cgctgatgcc ggtgcaggcc atcgacccag aggtacacag gctggtggag
      901 ggcgggccgc aggagcgacg gcgatacctg gattggggtg tgttccacgt ggaaccaggc
      961 ttcgtcgagc actggcgtcg gtaccagcgt gcgctgcgtc aacgcaatgc cagcctgagg
     1021 gccgggcagg ccgaggccct ggtgcgggcc tgggacgagg agctgatcgc cgccggcgag
     1081 gaggtcgccg cctttcggcg ccgatacgtg gcgggcctgc agggcgcgct acgggctgcc
     1141 gggcaaagcc tgctcggcgc ggacgtggac atcagtttgc atcagggctg gtccgcggag
     1201 cgcagcctag cccagtcgct ggagcaatca ctgccgcgcg accgggagcg cggcctcacg
     1261 cacatagggc ctcaccgggc agatctcgcg atccggatgg atggccagct ggcccgggac
     1321 cgggtgtcga ggggccagca gaaattgctc gcgtccgcga tgctcctggg gcagcttgcg
     1381 tatgacgccg aacgaggatc cggcgttgca gcgctacttg tggatgaccc agccgcggag
     1441 ctcgacgcgg gcagcctgga tcgtctgctc ggacaggtcc tgaagctccc cgcgcagttc
     1501 ttcgtgacgg cactggaccc gcgcaacgca tgcctcgagc gcttgccggc agggcacagg
     1561 ttccacgtgg aacacggctt ggtgacgcgt ctgatataat tccgcgtctt ttcaagctgg
     1621 atttcacatg agcggtgccg acgtctacga ttccagcaaa atcaaggtct tgaagggtct
     1681 cgacgccgtc cgtaagcggc ctggaatgta catcggggac accgatgacg gcaccggcct
     1741 gcaccacatg gtgttcgagg tcgtcgacaa ctcgatcgac gaggcgctgg ccggttactg
     1801 caccgacgtc tcggtcacca tccacgagga ccagtcggtc accgtgaccg acaacggccg
     1861 tggaattcct gtggatatcc atccggagga gggccgctcc gcagcggaag tgatcctcac
     1921 cgtgctgcac tccggcggca agttcgacga ctcgtcatac aaggtctcgg gtggtctcca
     1981 cggcgtcggc gtgtcggtcg tcaacgcgct gtccgagacg ctcctgctga cgatttatcg
     2041 cgacggccag gagtaccggc aggaataccg actcggcgag ccggtcgcgc ccctggcggc
     2101 cttcgggccc agcgatcgcc gcggcacgac catccgcttc aagcccagcg acacgatctt
     2161 cagcaacatc gagttcgagt acgagatcct gcagaagcgc ctgcgcgaac tgtcgttcct
     2221 gaattccgga gttcgcatcg aactcgtcga cgaaagggac gatcgtcggg aaatgttcca
     2281 ccatgaaggc gggatccggg agttcgtcag gtacctgaat cggagccgga cggcgattca
     2341 cgagtcaatt ttctggttcc gcggcgaacg tgacggtatc ggcgtcgaac ttgctctgca
     2401 atggaacgat tcgtacgcag agaccatgtc ctgctacacg aacaacatcc cgcagcggga
     2461 cggtggaacg cacctggccg ggttccgggc ggcgctgacg aagacgctca acgactacat
     2521 cgagagcgaa ctggcaagca agcgggacag ggtaccgacc accggcgacg actcgcgcga
     2581 gggtctcacg gcggtgctga gcgtgaagct gccggacccg aagttctcct cgcagacgaa
     2641 agacaagctg gtctcgtccg aagtgaaggg cgtcgtcgag tcgatcgtcg ccgagaagtt
     2701 gagcgagttc ctgctcgaga atccggcgca ggcgaaggcc gtcgtcaaca agatcatcga
     2761 cgcggcgcgt gcgcgcgagg ccgcgcgcaa ggcccgcgaa ctcacgcgcc gcaagggtgc
     2821 cctcgacatc gcaggcctgc ccggcaagct cgcggactgc caggagaaag atcccgcgct
     2881 gtcggagctg ttcatcgtcg agggtgattc cgcaggcggc tcggccaagc aggggcgcga
     2941 ccggcgcacg caggcgatcc tgccgctcaa gggcaagatc ctgaatgtcg agcgtgcgcg
     3001 cttcgacaag atgctgtcgt ccgccgaggt cggcacgctc atcaccgcgc tcggctgcgg
     3061 catcggcaag gacgacttcg acatcgcgaa gctgcgttac caccgcatca tcgtcatgac
     3121 cgatgccgac gtcgatggct cgcacatccg cacgctgctg ctcaccttct tctaccggca
     3181 gatcccgctg ctcatcgagc gcgggcacgt gttcatcgcg cagcccccgc tctacaaggt
     3241 gaagaagggc aagtccgaga cgtacgtgaa ggacgacggc gaactgaatc gcatgctgct
     3301 gcgctcggcg ctcgaggatg ccgagctgca cgtgaacgcc ggcgcggaag cgatgtccgc
     3361 cggcgcgctc gagaccctgg cgcgccgcta catggagttc caggccgccg ccaagcgctc
     3421 gccgcgacgg tacgacctgc acgtgctcga gcagatgctg cagctgcccg agttgtcggc
     3481 ggcggaccgt gaggacgccg ccaagctcag cgcctggggc gcgcagctcg agacgatgct
     3541 gaatgtcgcg cagcccgccg gccgcaagta caccgtcctg gtgcaccagg gcacgccgcc
     3601 gcacctggtg gtgagccgca ccgagcacag cgtcacggtg gagaagcacc tgcaccggga
     3661 gttcttcagc tcgagcgact accgccgcat cgccgagctg tcccgcacgc tggccggcct
     3721 gttcggcccg ggcgcgcacg tgaagaaggg cgaggccatg caggaagtca ccagcttccg
     3781 cgaggccctg ctgtggatgc tggagcaggc acgcaagggc cagtcgatcc agcgctacaa
     3841 gggcctcggg gaaatgaatc ccgaccagct ttgggacacg accatcaatc ccgagacgcg
     3901 ccggctcgtg caggtgaaga tcgaggacgt gctcggcgcc gacgagatct tctccacgct
     3961 catgggtgac caggtcgaac cacggcgcga gttcatcgag cgcaacgcgc tggccgcgac
     4021 caacatcgac gtctaggcga gcgaatctcg accgcagccg gtctggggcc gacgcgactg
     4081 tgtcatctgt cccggtcgcc gtcgacacac tctcaggcag gccatagtta ccaaataata
     4141 tttggtattg gctgattcat gcgcacgcta attaacaaca taatttagaa tatctacacg
     4201 acactagatg cctagcgacg accagaaatc gcaccgcgcc gtgttgacac cgtccgccat
     4261 cccgagcgcc gtcgagtcca gtctcaggta gttgtcgcgc gccgggtcgt actgggtcca
     4321 cgccggcgcg ccgctcgccg cgggtgcacc ggtggccgcg aagcctcccc agtagtgctg
     4381 catgttcgac gacagcgcgg tctcggcggc ggtcggcacg tagccgcgga tgttcatcac
     4441 gccgaacagg tagaacagct cgagggcgtg gaacgagccg aacaggggag cattggggcc
     4501 gtccggtacc tcggtgaaga aataacgaaa caccggctcc gactggctgg ccgtcagcgc
     4561 gcgggcgatc gatcgcgccg gacacgtgaa tttgacgtcc gacgtgagcg cgacatacgc
     4621 ggcacgtgac gtgccgaagt tcgcggccga gtacaggctc gcgacctgtg cccggatcgc
     4681 gggatcctgg accgtcgaat tcaggaaggc agcgtagtcg gcgtccgaaa aggtctgcgg
     4741 gacttcgagg ccggtctcgt ccgcattcgt gccgatcacg acggggacgc ggttctggag
     4801 gccgtcgcgg atcgcctcga gcgggatcgc gggcaatgtc gtgccgtcga cgctcggctg
     4861 atagggcgcc tgcgcaccgg cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4981 ngtcgccgcc gaacgcaccg atgtcgcgct gcacccactt caatgccgcg accatgtcct
     5041 cgagtccata gttgccggat cgcgcgtccg gcgcctcggc gtcgagtccg ggaagcacga
     5101 gccagccgag cggtccgagg cggtattgaa gcgtcacgac gacgtttccg gtcgcctcgg
     5161 cgagcgcctt gccgtcgtac aggcgcttgt tgccgtcgat gacctcgctc gcggagccca
     5221 tcgagttgcc gccgccgtgg atgaagacca tgaccggcgc gagtccggac gggaagctgg
     5281 acttcggggc gaatacgttc acggtgaggc agtcctcgtt gccgacggga ctgccggtgg
     5341 tgcggtcgaa ctgcgggcaa gcctgcgcat acgtgcctgc cggcacgttg ccggccgggc
     5401 agggcggtgc ggcgggcgaa cgccagcgca gcgatccgac gggagctgcc gcgtagcgga
     5461 tcccgaggaa ggaccagctg ccgccccggt cggtgccgac gtagctgccg caggatgtcg
     5521 tggccagtcc atccgccggt ccgacgggcg ggggagcgcc tccggagccc gacccggagc
     5581 caccgccgcc accgcaggct gaaagcagca gggcgagcat cgtcgtgcga aatgcgcgca
     5641 tgggcatccc ctggagaatc cggctgccgt cagactgcca acgctgccgg cccggcccag
     5701 tttacagccg accgtccgga cccgcggcgt aggccgccgg tgggctggtc actcctacgg
     5761 atgacaaagt gcgaaaccag tgcaacattt cgctgtcgga ccggcctaaa cgagagccgc
     5821 cgtcgatggc aggaggggac catggatcgc ctcgacaccg atgcacgcca ggatttccgt
     5881 ccgcacgagc acgcgatgga ggcgccggcg cggtcgccac ggccgtccat gtccgggcgc
     5941 ctcgttcgca gcgtgctgac caggctcggc gacccgcgga tcgaattcga gctctggacg
     6001 ggggagcgga tccgcacatc gcttgaaccg ccgctcggtc ggctgatcat ccgcgacgtc
     6061 cggaccctgc gcgagatctg catgcgcggt gaagtgggca tgggagacgc ctatgccgac
     6121 ggcgggctcg aggtggacgg cgatcttgca tcgatcctcg aggacgtcta cctgcattcg
     6181 ccgcgacgtg ccgacgacac aggacggccc gcggccgcga cccggcggtc acgacgcgcg
     6241 catcgcaaca cgctcgtggg ctcccgggac aacatccacc accactacga catcggcaac
     6301 gcgttctact cgctgtggct cggcgagacg atgtcttaca cctgcgcgta ctacccgacg
     6361 cccgacgcga ccctggacgc ggcgcaggtc gcaaagatgg atcacgtctg ccgcaagctg
     6421 cggttgcagc cctccgagca cgtattggaa gctggcttcg gctggggcac gctggccacg
     6481 cacatggcca gtcgctacgg cgtgcgagtc cgcgcgtaca acatctcgga ggagcaggtt
     6541 cgattcgccc gcgagcgcgc tgccgggctg gggctgggcc ggcaggtcga gtacgtccac
     6601 gatgactacc gcaatgcgac cgggccgtgc gacgcgttcg tctcggtggg catgctcgag
     6661 cacgtcggca ccgagaacta tgcgacgctc ggccacgtca tctcgcggtg cctgaagccg
     6721 aacggccgcg gcctggtgca ttcgatcggg cgtaaccagc cggagcctct gcatccctgg
     6781 atcgagcggc gcatcttccc cggggcctac ccgccgtcgc tcaaggagat gatggacatc
     6841 ttcgagccct ggaatctctc cgtgctcgac gtggagaacc tgcggctgca ttacgcgcgc
     6901 acctgccggc actggctcga ggcgttcgaa gcggcgcgcg accgggttgc cgcgatgttc
     6961 gacgagcgct tcgttcgcat gtggcggctg tacctcgccg gctcgacggc ggcattccgc
     7021 accggggagc tgcagctgtt ccaggtcgtg ttcgcgccgg gccgcagcaa tgacgtgccg
     7081 tggacgcgag ccgacctcta cgcgcggtaa gccagcggga gtgcgatgca gtcgtgtgag
     7141 gcattgatcg tcggcggcgg accggcgggc tcgacctgcg cgcgagctct cacgcgcttc
     7201 gggctcgacg tggtcgtgat cgacaaggcc gggttcccgc gcgacaaggt ctgcgccgga
     7261 tgggtcacgc cgccggtgct cgagacgctc gagatcgacg tcgacgatta ccgcaacggc
     7321 cgcgtgctgc aggacatcac gggcttccgc accggagtca tcggcggcgg cagcgccacg
     7381 acgcgctacg acggcccggt gagcttcggg attcgtcgct gcgagttcga cgacttcttg
     7441 ctgcgacgtt gcggtgcccg cctgcggctc ggcgagacgg tgacgaccat cgagcgagtt
     7501 gcggatggct gggtcgtcaa cggaacgctg agcacgcgga tcctggtcgg tgcgggcggc
     7561 catttctgtc cggtcgcgcg cctgcttgga acgaacgtcg ggcgggcgga gtcgaccgtc
     7621 gtcgcgcagg agatggagtt cgagctcgac ggcgcagggc cgggcgacgt cgaggcgggt
     7681 cagccggaac tctatttctg ccgcgacctg agcggctacg gatggagctt ccgcaagggg
     7741 acgtacctga acgtggggct gggccgcgag aacggccacg gaatgtcgga ggccgtccgc
     7801 gaattctgga aatggctcga ggcccagggc aaggtgcccg cgggcatggc cccgcgaatg
     7861 aagggccatg cgtacgtgtt gtacggccat gcctcacggc cgcgggtcgc ggatcgtgtc
     7921 gtcgcgatcg gtgacgcggc cggcctcgca tacgcgcaga gtggcgaggg aatccggacg
     7981 gccgtcgagt ccggtgtgct cgcggcccgg gccatcgttg ctgcaaaggg cgactactcg
     8041 cgagcggcct tgtcccgcta tgaagccgcg atcgaagagc gattcggacg cgccgctcca
     8101 tcggcggcga gcggacaacc gccggggagg ctgcgagcgg cggctggccg ctggctgctg
     8161 tcgcggccgt ggttcgtgcg ccgcgtcgtg ctggatcgct ggttcctgca cgcgcacgta
     8221 ccggcgctgg aggtccgggc ggtctgagcg gcttcgcctc gaagccagcc ggcgatcgcc
     8281 aggccaaccg gccggtccgg ccgcgtcgtc atttggttat tccgaaatcc tgaacggagt
     8341 aatcggattg agctggttta tccgaccagt tgcagggcgt agcttgtgcc ccgtcacgaa
     8401 cggaggatca agccatgttc acccgccact cgaccgaccg cctgctttcc gaagtgcttg
     8461 ccggcctcgc gatcacggtc gccgcggtgt tcgccgcgat gacttacgcc gtggtgcatg
     8521 cacagagctt cgcctgatcc aggtcagtcc gtccggaatc ccgccttgcg gtggctgccg
     8581 tcgcagaacg gcttgttcgc ggacgcgccg cagcggcaaa gatagacctc ggtggtgcgc
     8641 ttgatcgtgc gaccggtgcc gctcacgatt tcgagtgatc cggtgacctt gagcggtccg
     8701 ttcgcctgcg gcgcaatctc gagcgtccca tctcgattca cgagcggcgc cgattccgtc
     8761 gtcgccggct cgccgctggc gacaaatcca atcctgccgt ggctgccgtc gcagtacggc
     8821 ttgtgcgccg acgcaccgca gcggcacagc gtcgcacgca gcgcggtcgc atcgccgatc
     8881 ttcatgtcgg catggaagcc gagcggcccg ttctcacgta ctcgaaccgt gttgaccagc
     8941 ggcgggcgct cgttcggccc agcgtcgtgg cgctcgtagg tgatcgcgcc ggacgggcag
     9001 ttggcggcga gtgccgccac ttcgtccggc gtcgcgttgt cggggtgtat ccactctccc
     9061 gcgacgttcg gcacgaacac gtcgggccgg tcgaggacgc aatggcgcga gtggatgcac
     9121 ttgcgggcct cgaagtgaat cgtcacgtct cggccgcgaa cggattcctt gctgctggtc
     9181 atggggactc ccggtggcgt gtgccgtagt ctagtccccg tgcgacccgg acctgtcgaa
     9241 acggggcctg gcgcctgtct cagtccggct tgccgcccgg ctgggcgacg atctccgcaa
     9301 tcttgccctc gatccactgc tgcgcgcggg cgttgatctc gcgtggatca agccccaagg
     9361 ggtcgatcgg cgggccgatc acgacgcgga tcgtgcccgg gctcttgagg atgctgcggc
     9421 ggccccagaa gtaacccgag ttgtgcgcga tcggcacgac cggcgcgccg gtctgcgtgc
     9481 ccagcagtgc gccgctgatg ccgtacttgc gcgtctggcc cgccgccacg cgcgtgccct
     9541 cggggtacac gatgattccc atgccggccg cgagccgctc gcggccctgc gcgatgacct
     9601 ggttcaccgc cgagtgcccg gcgctgcggt tgatcgcgat cggcttgtag gtcttcaccg
     9661 cccagccgac caccgggatc caggtgacct cgcgcttcaa gagccaggac gctggcggca
     9721 cgacgaacat ctgtgccagg gtgtcccagc tcgacgagtg cttccagagc gagatgaacg
     9781 ggcggtccgg caggttctcc cggccttcga cgacgtagtg caggccgcag atcgcgccgg
     9841 cgagccaggt gaggaacagg ccccacgtgc gcgggatcac gtagcgctgt tccaggctga
     9901 ggggcagcac cgcggcgatg agcaccgcga atccgaacag cagcgtcacc gcgaagaaca
     9961 ggatcgtgta gaggagcgac cgcaggtact gcatcggggc ctacccgggc aacctcgaca
    10021 ggtcggcggc gtgcatgaac agcgcgcgca ggcgcccgag cagcgcgagg cgatttcccc
    10081 gcagcgccgg gtcctccgcc atgaccatca cggaatcgaa gaattcgtcc acggccttgc
    10141 gcagcacggc caggctctgc agcgcctcgg tgtattcacg cgcgcggaac ttcggctcga
    10201 ccaggcgtgc caccgccagg acctgctcgg ccagcacctg ctccgccgga tcgcgcagca
    10261 gcgagtcgtc gatcgcttcg ccgatcggac caggggcctt gcgcaggatg ttggcgatgc
    10321 gcttgttggc cgcggcgagg ctggcggcgt cgggcaggcc gaggaaccgg gcgagcgcgc
    10381 gcagccgcga atcgaagtcg agcggcgacg ccgggcgcgt cgcgagcacc gcatcgaaca
    10441 tctcggtggt gacggtcatg ccggcctcgc cgtcgaggta atacgcgcgc aggcgctcga
    10501 agacgtagtc gtagacgtcg cgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnngacttgc ggtccgtggt ccagaagaac gccgcgtcgc tgagccgagg ccgcaccacg
    10681 cgctcgttgc ccgcgatgac ctgctcggga tccgcgcttt cgatgttcgc aacggtgacg
    10741 aaccacggca tcagcttccc gctgctgtcg cgcacgggga agtaccgctg gtggtcctgc
    10801 atggtcgcga tcggcacctc ggcgggcagc tcgaggaact gcgcgtcgaa gcgcccggcc
    10861 agcgggactg gccactcgac cagcgcggtc acctcgtcga gcagcgactc gtcgatcacg
    10921 gcctcgccct gcagccgcga cgcgaccgct gccacgccct ggcggatggt ctcgcgccgt
    10981 tcgtggatgt cggccatcac ccggccgcgc ttgcgcagga ccgcgacgta tgctgacggg
    11041 gtgccgacac gcagggcctt cggcgccatg aagcggtggc catacgtcac gccgcccgcg
    11101 ggcacgccga ggatttcgca cgggatctcg tcgcgtccga acagcatcag cacccagtgc
    11161 accggccgca cgaattctgc cgtgccggca ccccagcgca tccgcttcgc gatcggcagg
    11221 gcgtcgagcg cggactgcac gatgccgggc agcagcacgg aggtgcgcgc gccaggctcc
    11281 gtaccgcgat gcacgagcca tacgcccttg ggggtctcga gcttctcgag cgccgcaacc
    11341 tcggtgccgc agctcctcgc gaaggcgagc gccgcctgcg tcggcgagcc ctgcgcatcg
    11401 aaggccgcct tgagcggcgg tccccggcgc tcgatgttgc ggtccggttg ctgctcggca
    11461 agtcgccgca cacggacggc gaggcgccgc ggtgtcgcat agcgctcgac cgcgccgtgc
    11521 ttgacaccag cggtgtccaa cccgctcgct atgccgtccg cgaatgccga cgcgagcgtg
    11581 agcagcgatt tcggcggcag ctcttcggtg ccgatctcga ccaggaaatc gcgggccgca
    11641 gggttcatgc cagtccccgc gtcgtgaggc cccgctcggg cagcggtgga tcgaccatgg
    11701 ggacaggcac cg
//
LOCUS       DAOMFD010000053        11663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1437_length_11663_cov_32.131289, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000053 DAOMFD010000000
VERSION     DAOMFD010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11663
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1437_length_11663_cov_32.131289"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="secD"
                     /locus_tag="PL152_04800"
     CDS             <1..1104
                     /gene="secD"
                     /locus_tag="PL152_04800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQR48631.1"
                     /translation="RIVVQLPGVQDPNEALRVLGATATLEFRLVDDQNSPAEAERTKR
                     APLGSKLYHTREGQPVLLKRDVIVSGEQLVDASSGFSEGEPAVYVKLDARGASEMLRT
                     TRENLGRPMAVVFIEKKRVAEGEPCNGQRAGNDCTEEKVISVATIRGIFSSNFQITGL
                     MAGEARELALLLRAGSLAAPLYIVEQRTVGPSLGQDNIDRGVRALVIGMLATWVWMVL
                     YYRAFGLVADAVLLANVVLLTALLSMLQAALSLPGIAGIVLTVGMAVDANILIYERIR
                     EELRNGLSPHAAINAGFEKAFSSIADSNVTTLIAGVVLFTFGTGPVRGFAVVLSLGIL
                     TSMFTALVGSRALIHLVWGRRRKIERLSIGGGY"
     gene            1106..2041
                     /gene="secF"
                     /locus_tag="PL152_04805"
     CDS             1106..2041
                     /gene="secF"
                     /locus_tag="PL152_04805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HQR48632.1"
                     /translation="MEFFHKKTSYPFMGTRRRWYLVSTVVLIAALVSLFARGLNFGID
                     FTGGVALELGFPQAADLEQVRGALEQQGFTHAVVQSFGTSRDVMVRLPPEEGQDTNKV
                     AQAVLGAIKAHEPNVELRRTEVVGPQVGAELANKGALAVLFTFLLILAYVAFRFQWKL
                     GVGAIVAALHDPIVILGFFSETQMTFDLPALAAILAVIGYSLNDTVVVFDRIRERFLS
                     MRKGTPEQVIDSAINETLSRTIMTHLTTMITIMSLLVFGGEVLRGFSVALAIGVVVGT
                     YSSIYIASAIALDFKLTARDLMPVQREKGAVDDMP"
     gene            2082..3068
                     /locus_tag="PL152_04810"
     CDS             2082..3068
                     /locus_tag="PL152_04810"
                     /inference="COORDINATES: protein motif:HMM:TIGR03718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HQR48633.1"
                     /translation="MTELVWAGFIALFLALLFLDLSVLHREAAELSVRQALSWTLVWI
                     GVAASFTFVVYGLYEHGWFGFEPGEGVSGGQDAVLQFVTGYLLEWSLSVDNIFVMALI
                     FAYLKIPPLYQYRVLFWGIVGAIVLRGLMILGGTALLAHFDWMFYVFGATLLISALRM
                     LRDDEAERDVGSGLPARLIRRFVPVTDELDRARFFVRRDGRAYATPLFVALVMVELTD
                     VVFAVDSIPAILAVTRDPFLVFTSNAFAILGLRSLYFAVAGLMALFRYLKYSLVLILA
                     FVGVKMLLASAYHVPNLVSLGIILGTLAGGVVASMWATRRDRSAPGSGDPGF"
     gene            complement(3065..4513)
                     /locus_tag="PL152_04815"
     CDS             complement(3065..4513)
                     /locus_tag="PL152_04815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HQR48634.1"
                     /translation="MAHGLFTTKPISDLHEAGTGNELHRALDARALVMLGIGAIIGTG
                     IFVLTGTAAANHAGPALVLSFVIAGIGCAFAGLCYAEFAAMIPVSGSAYSYSYVTLGE
                     FVAWFVGWNLILEYLFAAATIAVGWSGYVVSLMNQLGVHLPDQLTNAPFTKGATHYSL
                     VATGAIINLPAVLILCVMSALCYIGIHQSARFNGIVVAIKVTVIVLFIGFGAWYVQGA
                     NWDPFIPPNTGPGHFGWSGVVTAAGIIFFAYIGFDAVSTAAQEAKNPQRDMPIGILAS
                     LAICTVLYISVAAVLTGMVPYLELNTPAPVALALDRYPALSWLAGWVKLGAIAGMTST
                     MLVMLLGQPRIFYAMARDGLLPPVFARVHPKFKTPSFSTVLTGVVAATVAGLLPVTIL
                     GELVSIGTLMAFVVVCIGVLVLRRTRPDLERPFRVKAPWLVCTAGALICSYMMIALPF
                     DTWVRLVVWTFFGLLVYFLYGRKHSKLDRRSS"
     gene            complement(4617..5417)
                     /locus_tag="PL152_04820"
     CDS             complement(4617..5417)
                     /locus_tag="PL152_04820"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQR48635.1"
                     /translation="MQPLLNIAVRAARRAGDLIVRNVDRVPTLGVRAKSRNDFVSEID
                     QLAERDIIETIRRTHPDHGFLGEEGGRSGGDEFVWIIDPLDGTTNFLHGFPTFAVSVA
                     CEHRGRLEHAVVYDPMRQELYTASRGDGAQLDGRRIRVSKQPGLEGALIGTGFPYRAN
                     AQWIDEYLAMLKAVMQQTAGIRRPGAASLDLAYVAAGRIDGFWEIGLNAWDTAAGTLL
                     ITEAGGHIGTLSGGEYRQGGNVIAGTPKVYEAMVECLRPFVPEALREG"
     gene            5441..6193
                     /locus_tag="PL152_04825"
     CDS             5441..6193
                     /locus_tag="PL152_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HQR48636.1"
                     /translation="MSIAIVLVGISHPGNIGSAARAMKTMGLTELRLVAPERFPATEA
                     TVMAAGADDVLASAQIHADVAGAVADCGLIVGTTSRARHLPWRIVEPRAAAVEIAAAA
                     RDGKVAILFGAERTGLSNEDIERCQLLVSIPTGSTYSSLNVAMAVQVVAYEVLVALRG
                     EAAAAAARGVPLASATEMERFYAHLEQVMEETGFHDRTGEGHLMARIRRLFNRAVLDQ
                     NEVNILRGILTSVQGKRRRAGEPHADQPEQPS"
     gene            6190..7368
                     /locus_tag="PL152_04830"
     CDS             6190..7368
                     /locus_tag="PL152_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HQR48637.1"
                     /translation="MTIVYLDNAASTPMDPRVIEAMLECCGPGGDFANPSATGHEPGR
                     RARARVEQARAEVAHLVGAAPEQVVWTSGATEADNLALFGAARFHRAKGRHIVTSRTE
                     HPAVLDSCRQLEREGFEVTYLKPGPDGIVQPSQVEEALRSDTIIVSLMHVQNEIGVVH
                     DVGTIGRICRARGVLFHVDAAQGAGKLPIDVGRDCIDLLAVTAHKVHGPKGVGALCVR
                     REPRLGLVPLLFGGGQERGLRSGTLPTHQIAAMGRTFAIAAEEMSLDAGRISGLRDWL
                     WRALSAVPGVLLNGHPTQRVAGILSVTIDGVEGETLLHALGDLAVSSGSACATTHAEP
                     SYVLRALGRSDRLAQSTLRLSLGRFTTREEVELAARRITTEVTRLRSIAPAAAPMPAA
                     "
     gene            7368..7760
                     /locus_tag="PL152_04835"
     CDS             7368..7760
                     /locus_tag="PL152_04835"
                     /inference="COORDINATES: protein motif:HMM:NF013738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HQR48638.1"
                     /translation="MSDPRYSAEVCRRVLELPGSAPLPADAVVAAGAAGDVEHGARVE
                     LRFQVAEGRILRGQFRAFGCPHVIAAASWVTDRLGGKGRDALATWDWREAADALEVPP
                     AKFGRLLTVQDAVRDAARNWPVTPGSTV"
     gene            7790..8113
                     /gene="iscA"
                     /locus_tag="PL152_04840"
     CDS             7790..8113
                     /gene="iscA"
                     /locus_tag="PL152_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016537756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HQR48639.1"
                     /translation="MAVTLTPAAADRVRNFLASRGRGVGLRVGVKKTGCSGYAYVVNY
                     ADEVAAGDVVFEAAGVKVVVDAESLRYVDGTEVDFVRHGLNEAFRFRNPNVRGECGCG
                     ESFNV"
     gene            8276..8701
                     /gene="ndk"
                     /locus_tag="PL152_04845"
     CDS             8276..8701
                     /gene="ndk"
                     /locus_tag="PL152_04845"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005409295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HQR48640.1"
                     /translation="MSVERTLSIVKPDGVRKNIIGEVYRRFENAGLRIVAARMMRLSQ
                     SEAEGFYAVHRERPFFKDLVSYMTSGPVVVQVLEGDNAVAKNREIMGATDPKKAAPGT
                     IRADLAESIEQNVVHGSDSLENAAREVAYFFAETELCPR"
     gene            8740..9858
                     /gene="rlmN"
                     /locus_tag="PL152_04850"
     CDS             8740..9858
                     /gene="rlmN"
                     /locus_tag="PL152_04850"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HQR48641.1"
                     /translation="MTDVAATKTNLLGLTRAGMEAFVSGMSEKPFRARQLMKWIYKRA
                     VGNFEEMTDLGKDFRRRLGEVAEIRVPEVILSQHSADGTRKWLLRFEGGQAIEMVFIP
                     EPGRGTLCISSQVGCTINCGFCSTGAQGFNRNLSTAEIVGQVWLANRELGYSPDGDRV
                     ITNVVFMGMGEPLMNYRNVVPAAEIMMDDLGLDLSRRRVTISTSGLVPQMLRIADETN
                     VALAVSLHAPNDELRSELMPINRRHPIASLLEACWHYVEKQNARSVTFEYVMLDGVND
                     KPEHARQLAALLRGRPAKVNLIPFNPFPGTRYRRSSEAAIERFRDLLIKGGVIATIRR
                     TRGDDIDAACGQLVGRVNDRTTARLGERNLLPASVTHA"
     gene            9855..10610
                     /gene="pilW"
                     /locus_tag="PL152_04855"
     CDS             9855..10610
                     /gene="pilW"
                     /locus_tag="PL152_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR02521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus biogenesis/stability protein PilW"
                     /protein_id="HQR48642.1"
                     /translation="MKRILAGLVLALVFTSAVALEQESQVDIKKAGEANAQLALAYIR
                     QGDLATAREKLEKAMDQNPDSVTTQLAAGFLYDRLGDDRKAQSHFEQAVKLSRDDPSV
                     LNSYGVFLCLKVDKKKGEAILLKAAASPMNRNPEFAYANAGRCARADGRPKDAEQYLR
                     KALGYKADQLDALFEMASLEHELGNDLAARAFLERYAAAAPVSAASLWLGYRIERGLG
                     DNAQAALYADRIKRDYPMSAELTALLDAEKAQK"
     gene            10607..11434
                     /locus_tag="PL152_04860"
     CDS             10607..11434
                     /locus_tag="PL152_04860"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR48643.1"
                     /translation="MTRAEAEIAELPTDPGARLRRQREAAGLTQQDVAERLNLDSGVI
                     VALEGDDFASLGAPVFARGHLKRYATLLGVAEAEIVGAYERSRTQLAQPTLIPKAREE
                     MAPVRARPVWPWLAGGAALFLVAAGLAAYLAGSFGKTQGTADASASGPATSGAAGAPA
                     GATTARPSPATAAAAAAMPADGATIAAPPGPVALAFDFAADSWVEVYDGSGTVVLYDL
                     GRSGTRRFATGAGPLRVTLGNPSSVRLSVNGRPAALPVPPAGQTVVRFNVGAAGPSR"
     gene            11452..>11663
                     /locus_tag="PL152_04865"
     CDS             11452..>11663
                     /locus_tag="PL152_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010257545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HQR48644.1"
                     /translation="MADTIPPIRGMNDVLPAESVAWQRLESVARETFAEYGYREIRLP
                     LLERTELFKRSIGEMTDIVEKEMYTF"
BASE COUNT         1793 a         3931 c         4128 g         1811 t
ORIGIN      
        1 cgcatcgtcg tgcagctgcc gggcgtgcag gacccgaacg aggcgctgcg cgtgctcggc
       61 gccaccgcga ccctcgagtt ccgcctggtg gacgaccaga actcgccggc ggaggccgag
      121 cgcaccaagc gggcgccgct gggctcgaag ctctaccaca cgcgcgaagg gcagccggtg
      181 ctgctcaagc gcgacgtcat cgtgtcgggc gagcagctgg tggacgcgag ttccgggttc
      241 tcggaagggg agccggccgt gtacgtcaag ctcgacgcgc gcggcgcctc cgagatgctg
      301 cggacgacgc gcgagaatct cggccgcccg atggcggtgg tgttcatcga gaagaagcgc
      361 gtcgccgagg gcgagccctg caacgggcag cgcgcgggca acgactgcac cgaggagaag
      421 gtcatcagcg tcgcgaccat ccgcggcatc ttcagcagca acttccagat cacggggctc
      481 atggcgggcg aggcgcgcga gctggcgctg ctgctgcgcg ccgggtctct ggccgcgccg
      541 ctctacatcg tcgagcagcg caccgtcggc ccgagcctcg gccaggacaa catcgaccgc
      601 ggcgtgcgcg cgctggtgat cggcatgctc gccacctggg tgtggatggt gctgtattac
      661 cgggcgttcg gcctggtcgc cgacgccgtg ctgctcgcga acgtcgtgct gctcacggcg
      721 ctgctgtcca tgctgcaggc ggcgctgtcg ctgccgggca tcgcgggcat cgtgctcacg
      781 gtcggcatgg cggtggacgc caacatcctg atctacgagc gcatccgcga ggagctgcgc
      841 aacgggctga gcccgcacgc ggcgatcaac gccggcttcg agaaagcgtt ctcctcgatc
      901 gccgactcga acgtcacgac cctgatcgcg ggcgtcgtgc tgttcacctt cggcacgggt
      961 ccggtgcgcg ggttcgccgt ggtgctgtcg ctcggcatcc tcacgtccat gttcacggcg
     1021 ctggtgggca gccgcgcgct gatccacctc gtctgggggc ggcgccgcaa gatcgagcgc
     1081 ctgtcgatcg gagggggata ctgagatgga attcttccac aagaagacca gctacccctt
     1141 catggggacc cgccgccgct ggtacctggt gtcgacggtg gtgctgatcg cggccctggt
     1201 ctcgctgttc gcccgcggcc tcaatttcgg catcgacttc accggcggcg tggcgctcga
     1261 gctcggcttc ccgcaggcgg ccgacctcga gcaggtccgc ggcgcgctgg aacagcaggg
     1321 cttcacccac gcggtggtgc agagcttcgg cacctcgcgc gacgtgatgg tgcgcctgcc
     1381 gcccgaggag ggccaggaca cgaacaaggt ggcgcaggcg gtgctcggcg ccatcaaggc
     1441 ccacgagccg aacgtggaac tgcgccgcac cgaggtcgtg gggccgcagg tcggggccga
     1501 gctcgcgaac aagggcgcgc tcgccgtgct gttcacgttc ctgctgatcc tcgcgtacgt
     1561 cgcgttccgc ttccagtgga agctgggcgt cggggccatc gtcgcggccc tgcacgaccc
     1621 gatcgtgatc ctggggttct tctccgagac gcagatgacc ttcgacctgc cggcgctcgc
     1681 ggccatcctg gcggtcatcg gctattcgct gaacgacacc gtcgtggtgt tcgaccggat
     1741 ccgcgagcgc ttcctgtcga tgcgcaaggg cacgcccgag caggtcatcg actcggcgat
     1801 caacgagacg ctgtcgcgaa ccatcatgac gcacctcacg accatgatca cgatcatgtc
     1861 gctgctggtg ttcggcggcg aggtcctgcg cggattctcc gttgccctgg cgatcggcgt
     1921 ggtggtcggc acgtactcgt cgatctacat cgcgagcgcg atcgcgctgg acttcaagct
     1981 caccgcacgc gacctgatgc ccgtgcagcg cgagaagggc gccgtggacg acatgccctg
     2041 aggccagcgg cggcttcagg cagtaacggg ggaaaaccgc cgtgaccgaa ctggtctggg
     2101 ccgggttcat cgcgctgttc ctggcgctgc tgttcctgga cctgagcgtc ctgcaccgcg
     2161 aggccgcgga gctgtcggtg cgccaggcac tgtcctggac gctggtctgg atcggcgtgg
     2221 cggcatcctt caccttcgtc gtctacgggc tctacgaaca cggctggttc ggtttcgagc
     2281 cgggcgaagg tgtctcgggc ggccaggacg cggtcctgca gttcgtgacc ggctacctgc
     2341 tcgagtggtc gctgtcggtc gacaacatct tcgtcatggc gctcatcttc gcgtacctga
     2401 agataccgcc gctgtaccag taccgggtgc tgttctgggg catcgtcggc gcgatcgtgc
     2461 tgcgcggact gatgatcctg ggcgggaccg cgctgctcgc gcacttcgac tggatgttct
     2521 acgtgttcgg cgcaacgctg ctcatctcgg cgctcaggat gctgcgtgac gacgaggcgg
     2581 agcgcgacgt cggctccggg ttgccggcac gcctgatccg gcgcttcgtg cccgtgaccg
     2641 acgagctcga tcgcgcgcga ttcttcgtgc gtcgcgacgg gcgtgcgtat gcgaccccgc
     2701 tgttcgtcgc cctcgtcatg gtcgagctca cggacgtggt gttcgccgtg gactcgatcc
     2761 cggcgatcct ggccgtgacg cgcgacccgt tcctggtgtt cacgtccaac gccttcgcga
     2821 tcctggggct gcgctcactg tacttcgcgg tggccggcct catggccctg ttccgctacc
     2881 tcaagtacag cctggtgctg atcctcgcct tcgtcggcgt gaagatgctg ctggcgagcg
     2941 cctaccacgt gccgaacctg gtctcgctcg gcatcatcct gggcacgctc gcgggcggcg
     3001 tcgtcgcgtc catgtgggcg acgcggcgcg accgctcggc gccgggcagc ggcgatcccg
     3061 ggttctagct gctgcggcgg tcgagcttgc tgtgcttcct gccgtacagg aagtacacca
     3121 gcaacccgaa gaaggtccac acgacgaggc gcacccaggt atcgaagggc agcgcgatca
     3181 tcatgtagga gcagatcagg gcgccggccg tgcagacgag ccagggagcc ttcacccgga
     3241 acggccgctc caggtccggc cgggtgcggc gcagcaccag cacgccgatg cacaccacga
     3301 cgaacgccat gagcgtgccg atggacacga gctcgccgag gatcgtcacc ggcagcagcc
     3361 cggcgaccgt ggcggcgacc acgcccgtca gcaccgtcga gaacgacggt gtcttgaact
     3421 tcgggtgcac cctggcgaac accggtggca gcaggccgtc gcgcgccatc gcgtagaaga
     3481 tgcgcggctg gccgagcagc atcaccagca tcgtggaggt catgccggcg atggcgccga
     3541 gcttgaccca gccggccagc cagctgagcg ccgggtagcg atcgagggcc agggccaccg
     3601 gggccggcgt gttgagttcg aggtacggca ccatgccggt gagcaccgcg gcgacgctga
     3661 tgtacagcac cgtgcagatg gcgaggctcg cgaggatgcc gatcggcatg tcgcgctgcg
     3721 gattcttggc ttcctgtgcc gcggtcgaca cggcgtcgaa gccgatgtag gcgaagaaga
     3781 tgatgccggc ggcggtcacc acgccgctcc atccgaaatg ccccgggccg gtgttcggcg
     3841 ggatgaacgg atcccagttc gcgccctgca cgtaccaggc gccgaagccg atgaacagca
     3901 cgatgacggt gaccttgatc gcgactacga tgccattgaa tcgggccgat tgatggatgc
     3961 cgatgtagca gagcgcggac atcacgcaca ggatcagcac cgccggcagg ttgatgatcg
     4021 cgccggtcgc gaccaggctg tagtgggtgg cgcccttggt gaacggcgcg ttcgtcagct
     4081 ggtcgggcag gtggacgccg agctggttca tcaggctgac cacgtatccc gaccagccga
     4141 cggcgatggt cgcggccgcg aacaggtact cgaggatcag gttccagccg acgaaccagg
     4201 cgacgaactc gccgagggtc acgtacgagt aggagtaggc gctgcccgag accgggatca
     4261 tcgctgcgaa ttccgcgtag cacagtccgg cgaaggcgca gccgatgccc gcgatgacga
     4321 acgacaggac cagcgccgga cccgcgtggt tggccgcggc cgtgcccgtc aggacgaaga
     4381 tgccggtgcc gatgatcgcg ccgatgccga gcatcaccag ggcgcgggcg tcgagggcgc
     4441 ggtgcagttc gttgcccgtg ccggcctcgt gcaggtcgct gatgggcttg gtcgtgaaca
     4501 ggccgtgtgc catgaagtcc cccgctcgag ttcgtggata ttgttcgcgc tggtccgggc
     4561 gccggcggaa tgtaccctga cggcggcccc gcgcgatagg ggcccgagtc ccggtttcag
     4621 ccctcgcgca gcgcctcggg cacgaacggc cgcaggcact cgaccatggc ctcgtagact
     4681 ttcggggttc cggcgatgac gttgccgccc tggcggtact cgccgccgga gagcgtgccg
     4741 atgtggccgc cggcctcggt gatgagcagc gttcccgccg ccgtgtccca ggcgttgagc
     4801 ccgatctccc agaagccgtc gatgcggcca gcagccacgt acgccaggtc cagcgacgca
     4861 gcgccgggcc gccggatgcc cgcggtctgc tgcatgaccg ccttgagcat cgcgaggtac
     4921 tcgtcgatcc actgcgcatt ggcgcggtac gggaaacccg tgccgatcag cgcaccctcg
     4981 agacccggct gcttgctcac gcggatgcgt cgcccgtcga gttgcgcccc gtcaccgcgc
     5041 gacgcggtgt agagttcctg tcgcatcggg tcgtagacca ccgcgtgttc gagccgccca
     5101 cggtgctcgc aggcaaccga caccgcgaag gtcgggaatc cgtgcaggaa gttggtggtg
     5161 ccgtccagcg gatcgatgat ccagacgaac tcgtcgccgc cgctgcggcc gccttcctcg
     5221 ccgaggaagc cgtggtccgg gtgcgtgcgc cggatggtct cgatgatgtc gcgctcggcg
     5281 agctggtcga tctcgctcac gaagtcgttg cggctcttcg cacgcacgcc gagcgtgggg
     5341 acccggtcca cgttgcgcac gatcaggtct ccggcgcggc gcgcggcccg gacggcgatg
     5401 ttgagtaggg gttgcatgcg ggcggctaga ataccagagc atgtccatcg cgatcgtcct
     5461 cgtcggcatc tcgcaccctg gcaacatcgg ctcggccgcg cgggcgatga agaccatggg
     5521 gttgacggag ctgcgcctcg tggcgccgga gcggtttcca gccacggagg cgacggtgat
     5581 ggcggccggc gccgacgacg tgctcgcgag cgcgcaaatc catgcggacg tcgcaggtgc
     5641 agtggctgac tgcggcctga tcgtcggcac gacgagccgg gcgcgccatc ttccctggag
     5701 aatcgtcgag ccacgcgcgg ctgcggtcga gatcgcggcg gccgcgcgcg acggcaaggt
     5761 cgcgatcctg ttcggagccg agcggacggg gctctcgaac gaggacatcg agcggtgcca
     5821 gctgctggtc tccatcccca ccggttccac ctacagttcg ctcaacgtcg ccatggccgt
     5881 gcaggtcgtc gcgtacgagg tgctggtcgc cctgcggggg gaagccgcgg ccgcggccgc
     5941 gcgcggtgtc ccgctcgcaa gcgccacgga aatggagcgc ttctatgcgc acctcgagca
     6001 ggtgatggaa gagacgggct tccatgatcg gacaggagaa ggtcacctga tggcgcggat
     6061 ccgccgcttg ttcaatcgtg cggtgctcga ccagaacgag gtcaacatcc tgcgcgggat
     6121 cctgacgtcc gtgcagggca agcgccgccg cgccggggag ccgcacgcgg accagccgga
     6181 gcagccatcg tgacgatcgt ctacctcgac aacgcggcgt cgacgccgat ggatccgcgg
     6241 gtgatcgagg ccatgctcga gtgctgcggg cccggcggcg actttgcgaa tccctcggcg
     6301 actggacacg agccgggacg tcgggcgcgc gcacgcgtgg agcaggcccg cgccgaggtc
     6361 gcgcacctcg tcggtgctgc gccggagcag gtcgtctgga catccggcgc cacggaagcc
     6421 gacaacctcg cgctgttcgg tgcggcccgt ttccaccgcg caaaggggcg gcacatcgtg
     6481 acgtcccgca cggagcatcc ggcggtcctc gacagctgtc gccagctcga gcgcgaaggc
     6541 ttcgaggtca cctacctgaa gcccgggccg gacggcatcg tgcagccgtc gcaggtcgag
     6601 gaggcgctgc gcagcgacac gatcatcgtc tcgctcatgc acgtgcagaa cgagatcggg
     6661 gtcgtgcacg acgtgggcac gatcgggcgc atctgcaggg cgcggggcgt gctgttccac
     6721 gtcgatgccg cgcagggggc gggcaagctt ccgatcgacg tcggccgcga ctgcatcgac
     6781 ctgctggcgg tcaccgccca caaggtgcac gggccgaagg gcgtgggcgc gctgtgcgtg
     6841 cggcgcgagc cgcggctggg gctcgtgccg ctcctgttcg gcggcgggca ggagcgtggc
     6901 ctgcggtccg gcacgcttcc gacgcaccag atcgccgcga tggggcgcac cttcgcgatc
     6961 gccgcggagg aaatgagtct cgacgccggg cggatctcgg gattgcgcga ctggctctgg
     7021 cgggcgcttt ccgcagttcc tggcgtcctg ttgaacggcc atccgacgca gcgggtcgcg
     7081 gggatcctga gcgtcaccat cgacggggtc gagggcgaga ccctgctgca cgcgctcggc
     7141 gacctggccg tgtcgtcggg gtcggcctgc gcgacgaccc acgccgagcc gtcgtacgtg
     7201 ctgcgcgcgc tcggtcgcag cgaccggctg gcacagagca cgctgcggct cagtctcggg
     7261 cggttcacga cacgggagga ggtcgaattg gcggcgcggc gcatcacgac cgaggtcacg
     7321 cggttgcggt ccatcgcgcc agccgccgcg ccgatgccgg ccgcgtgatg agcgatccgc
     7381 gctacagcgc cgaggtctgc cggcgcgtgc tggagctgcc gggcagcgca ccgctgcccg
     7441 ccgacgcggt cgtggcggcc ggcgcggccg gggacgtcga gcacggcgcg cgcgtcgaac
     7501 tgcgattcca ggtcgcggaa gggcgaatcc tgcgtgggca gttccgcgcg ttcggctgtc
     7561 cgcacgtcat cgcggcggcg tcgtgggtca ccgaccggct gggcggcaag ggtcgcgacg
     7621 cactcgcgac ctgggactgg cgcgaggcgg ccgacgcgct cgaggtgcca ccggcgaaat
     7681 tcgggcgcct gctcacggtc caggacgccg tacgggacgc ggcgcggaac tggccggtca
     7741 cacccgggtc tactgtgtag aatctcgcca ccccagggcg caacgaacca tggctgtcac
     7801 gctcacaccc gccgccgccg atcgagtccg gaatttcctc gccagtcgcg gacgaggcgt
     7861 cggcctgcgc gtgggcgtca agaagaccgg ttgctcgggc tatgcctacg tcgtcaacta
     7921 cgccgacgag gtcgcggccg gggacgtcgt gttcgaggct gccggcgtca aggtggtcgt
     7981 cgacgccgag agcctgcgct acgtcgacgg cacggaggtc gacttcgtgc ggcacgggct
     8041 caatgaggcg ttccggttcc gcaacccgaa cgtccgcggc gagtgcggct gcggcgagag
     8101 cttcaacgtc taactgcccg tcccgactga tcttttttat tggctcgccc cggacccgaa
     8161 ggtatacttc gcggttcgaa tccggcctcg ccgggacccg ggcagcgggt ccgcggtcgt
     8221 ggggcccaag tttcaccatc gcctggggcc gccggccccg accaagagga atcgcatgtc
     8281 cgtcgaacgc acgctgtcca tcgtcaagcc cgatggagtc cgcaagaaca tcattggcga
     8341 ggtctatcgc cgcttcgaga acgcggggct gcgcatcgtc gccgcgcgca tgatgcgcct
     8401 gtcgcagtcg gaggccgagg gtttctatgc ggtgcaccgg gagcgtccgt tcttcaagga
     8461 cctcgtgtcc tacatgacct ccggcccggt ggtcgtgcag gtgctcgaag gcgacaacgc
     8521 cgtggcgaag aatcgcgaga tcatgggcgc gaccgacccg aagaaggccg ccccgggcac
     8581 gatccgcgcc gacctcgccg agagcatcga gcagaacgtc gtgcacggct ccgacagcct
     8641 ggagaacgcg gcgcgcgagg tcgcgtactt cttcgcggaa acggaactct gcccgcgctg
     8701 agccggaact ccgcccccgt cccgcgagtc acctgacgca tgacagacgt cgccgccacg
     8761 aagacgaacc tgctcggcct gacacgggcc gggatggagg cgttcgtctc ggggatgagc
     8821 gaaaagccct tccgcgcgcg ccagctcatg aagtggatct acaagcgcgc ggtcggcaac
     8881 ttcgaggaga tgacggacct cggcaaggac ttccggcgcc gcctcggcga ggtcgccgag
     8941 atccgcgtgc ccgaggtgat cctctcgcag cactccgccg acggtacgcg caagtggctg
     9001 ctgcggttcg aaggcgggca ggctatcgag atggtcttca tcccggagcc cggacgcggg
     9061 acgctgtgca tctcgagcca ggtcggctgc acgatcaact gcggcttctg ctccacgggc
     9121 gcccaggggt tcaatcgcaa tctctcgacc gccgagatcg tgggccaggt ctggctggcg
     9181 aatcgcgaac tcggctattc gccggacggc gaccgggtca tcaccaacgt ggtgttcatg
     9241 ggcatgggcg agccgctcat gaactatcgc aacgtcgtgc cagccgccga gatcatgatg
     9301 gacgacctgg gcctggacct ctcgcgccgc agggtcacca tcagcacttc cgggctcgtc
     9361 ccgcagatgc tgcgcatcgc cgacgagacc aacgtggcgc tcgcggtctc gctgcacgcg
     9421 ccgaacgacg agctgcgcag cgagctgatg ccgatcaacc ggcggcaccc gatcgcgagc
     9481 ctgctcgagg cgtgctggca ctacgtcgag aaacagaacg cccgcagcgt caccttcgag
     9541 tacgtgatgc tcgacggggt caacgacaag cccgagcacg cccggcagct cgcggcgctg
     9601 ctgcgcggcc gtccggccaa ggtgaacctc attccgttca atccgttccc cggcacgcgc
     9661 taccggcgct cgagcgaggc ggcgatcgag cgcttccgtg acctgctcat caagggtggc
     9721 gtgatcgcta cgatccggcg cacgcgcggc gacgacatcg atgccgcatg cgggcaactc
     9781 gtcggtcgcg tcaacgacag gaccaccgcg cggctcggtg agcgcaacct cctccccgcg
     9841 tcggtgaccc acgcatgaag cggatcctcg caggtctcgt actggcgctc gtcttcacgt
     9901 cggcggttgc gctcgagcag gagagccagg tcgacatcaa gaaggccggg gaagccaacg
     9961 cgcagcttgc gctggcctac atccgccagg gtgatctcgc gacggcccgc gaaaagctcg
    10021 aaaaggccat ggaccagaac ccggactccg tgaccacgca gctggccgcg ggattcctgt
    10081 acgaccgtct cggcgacgac cgcaaggcgc agtcgcactt cgagcaggcg gtcaaactga
    10141 gccgcgacga cccgagcgtg ttgaacagct acggggtctt cctgtgcctt aaggtcgaca
    10201 agaagaaggg cgaggccatc ctgctcaagg cggccgcgag tccgatgaac cgcaaccccg
    10261 agttcgcgta cgccaacgcc gggcgctgcg cccgtgccga cggtcgtccg aaggacgccg
    10321 agcagtacct gcgcaaggcg ctcggctaca aggccgacca gctcgacgcg ctgttcgaga
    10381 tggcgtcgct cgagcacgag ctcggcaacg acctggcggc ccgcgccttt ctcgagcgct
    10441 acgccgcggc tgcgcctgtg tcggccgcgt cgctgtggct cggctaccgc atagagcgtg
    10501 gcctgggcga caacgcccag gctgccctgt acgccgaccg gatcaagcgc gactacccga
    10561 tgtcggccga gctgaccgcc ctgctcgacg cggagaaggc gcagaagtga ccagggcgga
    10621 agcagagatc gccgagctgc cgacggatcc cggggcgcgg ctcaggcggc agcgggaggc
    10681 ggccggactg acccagcagg acgtcgccga gcggctgaat ctcgacagcg gcgtgatcgt
    10741 cgctctcgaa ggcgatgact ttgcgtcgct cggcgcgccg gtcttcgcac gcgggcacct
    10801 gaagcgttac gcgacgctgc tcggcgtcgc cgaggccgag atcgtcgggg cctacgaacg
    10861 ctcgcggacc cagctggcgc agccgacgct gattccgaag gcccgcgagg agatggcgcc
    10921 ggtgcgcgca cgtccggtgt ggccttggct cgcaggtggc gcggcgctgt ttctcgtggc
    10981 cgccggcctt gcggcgtacc tcgccgggag cttcggcaag acgcaaggca ccgccgacgc
    11041 cagcgcgtcg ggcccggcga ccagcggtgc tgcaggggcg ccggccgggg cgacgaccgc
    11101 tcgcccatcg cccgccactg ccgcggcagc cgccgcgatg ccggcggacg gcgccaccat
    11161 cgcggcccca cccggaccgg tcgcgcttgc gttcgatttt gcggccgatt cctgggtgga
    11221 ggtctacgac ggctccggca cggtggtgct gtacgatctg gggcggtcgg gcacgcgacg
    11281 gttcgccact ggcgcaggac ccctgcgcgt gacgctgggc aacccgtcgt cggtgaggct
    11341 gtcggtcaac ggccggccgg ccgcgctgcc agtgccgccg gcgggccaga cggtggtccg
    11401 tttcaacgtc ggcgccgccg gcccgtcacg ctgatccccg gggaagggcg catggccgac
    11461 acgattccac ccatccgcgg catgaacgac gtgctgcccg ccgagtctgt cgcctggcaa
    11521 cggctcgaga gcgtcgcccg tgagaccttc gccgaatacg gctaccggga gatccgcctg
    11581 ccgctgctcg agcgcacgga actgttcaag cgctcgatcg gcgagatgac cgacatcgtc
    11641 gagaaggaga tgtacacgtt cga
//
LOCUS       DAOMFD010000054        11609 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1447_length_11609_cov_31.034966, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000054 DAOMFD010000000
VERSION     DAOMFD010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11609)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11609
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1447_length_11609_cov_31.034966"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="PL152_04870"
     CDS             <1..312
                     /locus_tag="PL152_04870"
                     /inference="COORDINATES: protein motif:HMM:NF014611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQR48645.1"
                     /translation="ALSSRNRYLTPEERERAPLLYRALDRARRRLESGDSDVAAIERE
                     GMEALGAAGFRPDYFEVRMAGTLARPHGHNVDVVVLAAARLSRARLIDNLQCRANGHL
                     G"
     gene            complement(254..1348)
                     /gene="queG"
                     /locus_tag="PL152_04875"
     CDS             complement(254..1348)
                     /gene="queG"
                     /locus_tag="PL152_04875"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HQR48646.1"
                     /translation="MNPNIDPAALAVQVKTWGRDLGFQKVGIAAVELPEDERRLVEWL
                     EAGHHGSMEYMARHGARRARPAELVPGTSRVISARMDYWPDATDAATVLGDATQGYVS
                     RYALGRDYHKIVRNRLARLAGQLATAVGVAGYRAFTDSAPVLEKALARDAGLGWIGKH
                     TNLLDRHDGSWFFLGEIFTDVPLPVDEPVASHCGSCTACIDVCPTRAIVAPYRLDARR
                     CISYLTIEHHGPIPEEFRAAIGNRIYGCDDCQLVCPWNRYAKTTTESGFHPRHGLDAS
                     RLTELFAWSEAQFEERLAGSAIRRIGHERWLRNIAIALGNAPHTPEVAAALERRRQHP
                     SEVVREAVEWALSRGGRSPGTAGCRSGVPC"
     gene            1390..>2042
                     /locus_tag="PL152_04880"
     CDS             1390..>2042
                     /locus_tag="PL152_04880"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HQR48647.1"
                     /translation="MHSLAGALYSASQVRDLDRRAIHDLGIPGYELMSRAGRATLDAL
                     CALWPTARSIAVLCGPGNNGGDGYVVARLARAQGLRVTVVALGDPGALTGDARRAYDE
                     FVAAGGRCEGWSPGALESDVVVDALFGTGLARALDGAAASMIAAVNASGRPVVAVDVP
                     SGLQADTGAVLGAAMHADLTVTFIGRKVGLYIGEGLDHVGRVLLDDLAVPPETYDGA"
     assembly_gap    2043..2142
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2143..3026
                     /locus_tag="PL152_04885"
     CDS             <2143..3026
                     /locus_tag="PL152_04885"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HQR48648.1"
                     /translation="RVLLDDLAVPPETYDGAIPLARLIGDATVAPALPRRARTSHKGA
                     HGHVLVVGGGPGMPGAARLAGEAALRAGAGLVTLAVHPESTGVVATRPELMCVAAKSA
                     RDIERALGRATVVAVGPGLGQGEWATELFGAVLASGLPLVVDADALNLLASAPRKSDH
                     WVITPHPGEAARLLDITGAAVQADRLAAARALQERYGGTAVLKGAGTIVHPPGGPPWI
                     CDRGNPGMAAGGMGDVLTGVIAGIAAQCGDLALAARAGVFVHAQAGDLAARAGERGML
                     ASDVLRQVRACVNPSCE"
     gene            3017..3481
                     /gene="tsaE"
                     /locus_tag="PL152_04890"
     CDS             3017..3481
                     /gene="tsaE"
                     /locus_tag="PL152_04890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HQR48649.1"
                     /translation="MRVTTADADATRALGARLANAFLAADEAGAIVVGLSGDLGAGKT
                     TLVGGLLGALGHDGATRSPTYTLVEPYRLAGRDVHHCDLYRLRDPSELDDLGLRDLMT
                     PRSLLLVEWPERAGGRLGAPDLSLVLAYADGGRTAELSAASEAGKRVLALIP"
     gene            3558..>4266
                     /locus_tag="PL152_04895"
     CDS             3558..>4266
                     /locus_tag="PL152_04895"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HQR48650.1"
                     /translation="MPARPAKTTAFLLAFAALFAATVASSAAPTRVTDVRLWSGPEGT
                     RLVLDLSAPAEFQVFAVENPDRIVIDLQNADLVSRKALPAGQGPVKAVRSGPQPNHGL
                     RLVLDLDAPHAMRSFSVGPDGNAGDRLVVELPPAGAATATATAAAAAGLPGAAGASAP
                     AAAATTARAPDGASPVKRLAQADVNARDLVIAVDAGHGGQDPGALGRSGTQEKDVTLA
                     IARRLAAAIDAEPGMRAV"
     assembly_gap    4267..4366
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4367..6097
                     /gene="mutL"
                     /locus_tag="PL152_04900"
     CDS             <4367..6097
                     /gene="mutL"
                     /locus_tag="PL152_04900"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HQR48651.1"
                     /translation="PASVVKELVENSLDAGARSIEIDVERGGAELCRVRDDGAGISAT
                     ELPLALARHATSKIGRIEDLESVRTLGFRGEALPSIASVSRTKIVSRTADAPVATAIS
                     VLDGVISDPAPQPHPPGTTVEVRDLFFNVPARRKFLRSERTELQQIERLVERLALSRF
                     DVGFRLSSARRALASHPPARDERAQDARVAAVLGEEFIANALKLDHEGSGLRLHGWFC
                     MPTYARSQADQQHFFLNGRPLRDKLIASAVRLAYRDVLYHGRQPAYVLYLEMDPARVD
                     VNAHPQKLEVRFREPGLVHDFIFRTLERALAETRPAQPAAGAIPAARFGAPPGDAAQR
                     TSALELYTALGGVRDEAGSTGRGGTAGVSQSAAPPRPGAELPTAEVPTAEHPLGYAIA
                     QLHGIYILAQAQGGLVLVDMHAAHERTTYERLKASLESRRVAAQPLLVPIVLNVSTAE
                     ADEAEEHHTLFDELGIEMSRSGPAQLIVRSMPALLPTADPAVLVRDMLADLREQGSGG
                     ARQALDRALGTMACHQAVRANRRLTIPEMNALLREMETTLRADQCVHGRPTWSFVGMD
                     DLDRLFMRGR"
     gene            6097..7047
                     /gene="miaA"
                     /locus_tag="PL152_04905"
     CDS             6097..7047
                     /gene="miaA"
                     /locus_tag="PL152_04905"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HQR48652.1"
                     /translation="MTTAARPVDAVLLMGPTCSGKSSLALQLAERFPVEIVSVDSAQV
                     YRGMDIGTAKPTSAIRSWVPHHLVDVCDPTESYSAGRFRRDALRLVAEIRARGRVPLL
                     VGGTMLYFRALTRGIAPLPEASPEVRARIDERARQHGWPALHAELAAHDPAAAASIRP
                     ADGQRIQRALEVLELTGRPLSELQRLAEPSPLAFASFVLMPVERAVLYRRIDQRFLDM
                     MSEGFLNEVRALRARGDLRADLPSLRAVGYRQLWAHLEGDCGLDTAIAAGQTATRNLA
                     KRQLTWVNADPSLCWLRTLEDQELAPIYGAMAAAAPAGAP"
     gene            7160..7417
                     /gene="hfq"
                     /locus_tag="PL152_04910"
     CDS             7160..7417
                     /gene="hfq"
                     /locus_tag="PL152_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HQR48653.1"
                     /translation="MAKGQSLQDPFLNALRKERIPVSIYLVNGIKLQGQIDSFDQFVV
                     LLRNSVSQMVYKHAISTIVPARNVRLPIGDEELQAEDAPAA"
     gene            7451..8860
                     /gene="hflX"
                     /locus_tag="PL152_04915"
     CDS             7451..8860
                     /gene="hflX"
                     /locus_tag="PL152_04915"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF008280.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HQR48654.1"
                     /translation="MFDRPGNGERAILVCVGIGHAPEPDRMEEFRALARSAGAEVLEV
                     LTATRKAPEPRFFLGTGKVAELRERLVALGADLVVVDHALSPGQERNLEKELTCRVLD
                     RSGLILDIFAQRARTFEGKLQVELAQLEHMSTRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLIGRRIKTLNDRLDKVLVQRETTRQERRRGAVPTVALVGYTNAGKSTLFNRLT
                     SAGAYAADQLFATLDPTVRRLRLAPGLDVALADTVGFVRDLPHELVAAFRSTLQEARE
                     ADLLLHVIDAADPERAERVTQVNEVLESIGAQSLPQIAVFNKIDRSGEAPHVDRDSDG
                     RVDRIWLSAHSGAGVDLLRDALHRQLGSDLHHCVLRVPPHAGRARAQLFEAGAVLSQR
                     PLDGGDIELEVSLRRRDLDRICREAGLDVPPAIEPPCAPDEAFLQSRGSADRDAAVAV
                     ATAEPDVPLTDRPLAIGSN"
     gene            8867..10012
                     /gene="hflK"
                     /locus_tag="PL152_04920"
     CDS             8867..10012
                     /gene="hflK"
                     /locus_tag="PL152_04920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HQR48655.1"
                     /translation="MAWNDPGRNRNPWGNRPDKGAADLDEVLRNLQKKLAGLFGGGGG
                     AGGESGGGDAAMRGFGVSTVVVVLLLVWAATGLYKVDAAERGVVTRFGKYVETTEPGL
                     RWHLPWPIESRQIVNVETVEGMDDQTRMLTSDENLVDINFAVQYRRAEPVAYAFSVRD
                     PQETLGEVSESAIREIVGQSTLDFVLEAGRQEITAKTRELIQRTLDSYKTGIEVTTVN
                     LQGVSVPEQVQSSQRDAIKAREDKERLALEAQAYSNDILPKAKGSAARLAADSQAYKS
                     RIVADAEGEASRFSQLAEAYRRAPAVTRKRLYLETMEEVLGHSNTVLVDTKGTGNMIY
                     LPLDKLTERRAPVQTLDQQRQATSASPQAPAEEPATTVESRRERGTR"
     gene            10012..10881
                     /gene="hflC"
                     /locus_tag="PL152_04925"
     CDS             10012..10881
                     /gene="hflC"
                     /locus_tag="PL152_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HQR48656.1"
                     /translation="MGSRSYLGVILAGFAALLVSMSAFTVRETDLAIKFRFGEIVNAN
                     YSPGLHFMTPLVNNVRKFDKRILTEDYPAEQFLTSEGKILKINFFVKWRISDVSRYYI
                     STSGGQEEVAARRLGEIVKDGLKGVIARRTIQQVVAAERSEFLGEVLKIAGANVGGLG
                     MTMIDVRVKSIELPEEVSESVFNRMRQDFARQAAQLRAEGSAISEQTRAEADRQRTEI
                     LANAYRDAEKVKGEGDATAADIYAKAYGREPDFYEFFRSLQAYRKSLGKQGDVLVLAP
                     DGEFFKYLKEAGR"
     gene            10904..11101
                     /locus_tag="PL152_04930"
     CDS             10904..11101
                     /locus_tag="PL152_04930"
                     /inference="COORDINATES: protein motif:HMM:NF021366.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HQR48657.1"
                     /translation="MQWGDLWAAFALYLVLEGLMPFLNPGGMKRTLAKLSDMPEGTLR
                     AAGLGSMIAGAILLYYVRGGA"
     gene            11125..>11609
                     /locus_tag="PL152_04935"
     CDS             11125..>11609
                     /locus_tag="PL152_04935"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HQR48658.1"
                     /translation="MARNLVVIGAQWGDEGKGKIVDLLTERAAAVVRFQGGHNAGHTL
                     VIEGVKTVLSLIPSGILHPGVQCLIGNGVVLSLEALLREADALVQKGVPVYDRLRVSP
                     SCALILPSHIAIDRARERAKGANAIGTTGRGIGPAYEDKVGRRAVRVADLFQRDRLAA
                     RL"
BASE COUNT         1728 a         3982 c         4031 g         1668 t
ORIGIN      
        1 gccttgagct cgcgcaatcg ctacctcacg cccgaggagc gcgagcgcgc gccgctgctc
       61 taccgtgcgc tggaccgggc gcgccgacgt ctcgaatccg gcgactccga cgtggccgcg
      121 atcgagcgcg aaggaatgga ggccctcggc gccgcgggct tccgtccgga ttatttcgag
      181 gtgcgcatgg ccggcacgct cgcgcgcccg cacggtcaca acgtcgatgt cgtggtgctc
      241 gccgccgcgc ggctcagcag ggcacgcctg atcgacaacc tgcagtgccg ggcgaacggc
      301 cacctcggct gagcgcccat tcgacggctt ccctgactac ctcgctcggg tgttgccgcc
      361 gccgttccag cgcggccgca acctcgggcg tgtgaggcgc atttccgagc gcgatggcga
      421 tgttccgcag ccagcgctcg tggccgatgc ggcggatggc gctgcccgca agacgttcct
      481 cgaactgcgc ttcgctccag gcgaagagct ccgtcaggcg tgatgcatcc aggccgtggc
      541 ggggatggaa accgctctcg gtggtcgtct tcgcgtagcg gttccacggg cagacgagct
      601 ggcagtcgtc gcagccgtag atgcggttgc cgatcgccgc gcgaaattcc tcggggatcg
      661 gcccgtggtg ctcgatcgtg aggtaggaaa tgcagcgcct cgcatcgagg cggtagggcg
      721 ccacgatcgc ccgcgtcggg cacacgtcga tgcaggcggt gcagctgccg cagtggctcg
      781 ccaccggctc gtcgaccggc aggggcacgt ccgtgaagat ctcgccgagg aagaaccacg
      841 aaccgtcgtg gcggtcgagc aggttggtgt gcttgccgat ccagcccaag cccgcgtcgc
      901 gcgcgagcgc cttttccagc accggcgcgc tgtcggtgaa cgcgcgatag ccagccactc
      961 ccacggccgt agccagctgt cccgcgagtc gcgccagccg attgcgcacg atcttgtgat
     1021 agtcgcggcc cagcgcgtat cgcgacacgt agccctgggt cgcatcgccg agcaccgtgg
     1081 ctgcgtccgt cgcgtcgggc cagtaatcca tccgtgcaga aatgactcgg ctggtgccag
     1141 gcaccagttc ggcaggacgc gcgcgacgag cgccgtgccg cgccatgtac tccatgcttc
     1201 cgtggtggcc ggcttcgagc cattcgacga ggcgtcgctc gtcctcgggc agttccaccg
     1261 cggcaatgcc gaccttctgg aaaccgaggt cccgccccca ggtcttcacc tggacggcga
     1321 gtgctgccgg atcgatgttc gggttcatcc gtgccgcggc gcgaggaagg cggccagtat
     1381 aatgcccgca tgcactcgct cgccggcgcc ctctactcgg cctcccaggt gcgcgacctc
     1441 gatcggcgcg ccatccacga ccttgggatc cccggttacg aactgatgtc gcgcgccggc
     1501 cgtgcgacgc tcgacgcgct gtgcgcgctg tggcccaccg cgcgatcgat cgctgtgctg
     1561 tgcggtcccg gcaacaacgg cggcgacggc tacgtggtcg cgcgactcgc acgtgcgcag
     1621 ggattgcgcg tcaccgtcgt tgcgctaggc gatcccggcg cgctcacggg tgatgcgcgt
     1681 cgtgcgtacg atgagttcgt agcggccgga ggccgctgcg agggctggtc tccgggggcg
     1741 ctcgagtccg acgtagtcgt ggacgcgctc ttcggcacgg ggctcgcgcg cgccctcgat
     1801 ggcgcggccg ccagcatgat cgcggcggtc aatgcgtccg ggcgacccgt ggtagcggtc
     1861 gatgtgccat cgggcctgca ggcagacacg ggcgccgtgc tcggcgccgc aatgcatgcg
     1921 gacctgacgg tgactttcat cggccgcaag gtcggcctgt acatcggcga gggactcgat
     1981 cacgtcggcc gggtgctgct cgacgatctc gccgtgccgc cggagaccta cgacggcgcg
     2041 atnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccgggtg ctgctcgacg
     2161 atctcgccgt gccgccggag acctacgacg gcgcgatccc cttggcgcga ctgatcggcg
     2221 atgcgaccgt cgcgcctgca ctgccgcggc gggcacggac gtcgcacaag ggcgcgcacg
     2281 ggcacgtact cgtggtcggc ggcggacccg gcatgccggg ggccgcgcga ctggctgggg
     2341 aagcggcgct gcgcgccggg gcagggctcg tgacgctcgc ggtccatccc gaaagcactg
     2401 gggtcgtggc gacgcgcccc gaactcatgt gcgtcgcagc caagtctgca cgcgacatcg
     2461 aacgtgcgct gggtcgcgcg accgtcgtcg ccgtcgggcc cggcctcgga cagggcgagt
     2521 gggccaccga attgttcggc gccgtactcg cgagtggtct gccgctggtc gtcgatgcgg
     2581 acgcgctcaa cctgctggcg tcggcgccgc gcaagtccga tcactgggtg atcacgccgc
     2641 atcccggtga agccgcacgc ctgctcgaca tcactggcgc agccgtgcag gcggaccgac
     2701 tcgcggcggc gcgcgcgctg caggagcgct acggcggcac ggccgtgctc aagggagcgg
     2761 gcaccatcgt gcatccgccg ggtggaccgc cgtggatctg cgatcgcggc aatcccggga
     2821 tggcggccgg cggcatgggc gacgtgctca cgggcgtcat cgcgggcatc gccgcgcagt
     2881 gcggcgatct cgcgctcgcg gcgcgggccg gcgtgttcgt gcacgcccag gccggcgatc
     2941 tcgccgcgcg tgccggcgag cgcggcatgc tcgcgagtga cgtgctcagg caggtccgcg
     3001 cctgcgtgaa tccttcgtgc gagtgacgac cgccgacgcc gatgcgacgc gtgcgctcgg
     3061 tgcgcggctc gcgaacgcgt tcctggcggc ggacgaggcg ggcgccatcg tcgtcggcct
     3121 gtcgggggac ctcggcgcgg gcaagacgac actggtgggc ggcctgctcg gtgcgctcgg
     3181 tcacgatggc gcgacccgca gcccgacgta cacgctggtc gagccctacc ggctcgcggg
     3241 acgcgacgtg catcactgcg atctctatcg cctgcgggat ccgtccgaac tcgacgacct
     3301 cggactgcgc gacctcatga cgccgcgcag cctgctgctc gtggagtggc cggagcgtgc
     3361 gggcggtcgg ctcggcgctc ccgacctgtc gctcgtcctt gcctatgcgg acggcggtcg
     3421 gaccgcggag ctgtcggccg cgagcgaggc agggaaacgg gtacttgcgc tcattccctg
     3481 agacgcggtg tgacgatcct ctcgtatcta cataatttca ttgaaatatc ccggtggatc
     3541 agggtagatt acgtcgcatg cccgcgcgcc cggccaagac aaccgcgttc ctgctggcat
     3601 tcgccgccct gtttgcggcg accgtggcgt cctccgccgc ccccacccgc gtcaccgacg
     3661 tgcgcctctg gtccggcccc gaaggcacgc ggctcgtgct cgacctgtcg gcgccggcgg
     3721 agttccaggt attcgcggtc gagaatcccg accgcatcgt gatcgacctg cagaacgccg
     3781 acctcgtgtc gcgcaaggcg ctgccagccg ggcagggacc ggtcaaggcc gtgcgatcgg
     3841 gcccgcagcc gaaccacggc ctgcgactgg tactcgacct ggacgcaccg catgcgatgc
     3901 gcagcttcag cgtgggaccg gacggcaatg ccggcgaccg cctcgtggtc gaactacctc
     3961 cggcaggcgc cgcaaccgct acggccaccg ccgcggcggc agccggtttg ccaggtgcgg
     4021 cgggagcgtc ggcgccggct gctgctgcga ccacagcgcg cgcaccggac ggtgccagtc
     4081 cggtgaaacg gctcgcgcag gccgacgtga acgcgcgcga cctcgtcatc gcagtggatg
     4141 caggacacgg cggccaggac ccgggcgcac tcgggcgcag cggcacgcag gagaaggacg
     4201 tcacgctcgc catcgcccgg cggctcgcgg ccgcgatcga cgcggagccc ggcatgcgcg
     4261 cggtgcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccgg cgtccgtcgt
     4381 caaggaactg gtcgagaaca gcctcgatgc cggcgcccgg tcgatcgaga tcgacgtgga
     4441 gcgtggcggt gcggaactgt gccgggttcg cgacgacggc gccggcatct cggcgacgga
     4501 gttgccgctc gcgctcgcac ggcacgcgac gagcaagatc ggtcgcatcg aggacctcga
     4561 gtccgtgcgc acgctcgggt tccgtggcga ggcgctgccc agcatcgcct cggtgtcgcg
     4621 cacgaagatc gtatcgcgca ccgcggacgc accagtggcc accgcgatct ccgtgctgga
     4681 cggcgtgatc agcgatccag cgccgcaacc gcacccgccc ggcacgaccg tcgaagtgcg
     4741 cgacctgttc ttcaacgtgc cggcgcgccg caagttcctc cgctccgagc gcaccgagct
     4801 gcagcagatc gagcggctgg tcgagcgcct cgcgctgagc cgcttcgacg tcggcttccg
     4861 cctgagttcc gcgcggcgcg cacttgcaag ccacccgccg gcgcgggacg agcgggcgca
     4921 ggacgcgcgg gtggctgccg tcctcggcga ggaattcatc gccaacgcgc tcaagctcga
     4981 ccacgagggc tcggggctgc ggctgcacgg ctggttctgc atgccgacct acgcgcgcag
     5041 ccaggcggac cagcagcact tcttcctgaa cggccggccg ctgcgcgaca agctgatcgc
     5101 gagtgccgtg cgccttgcgt accgcgatgt cctctatcac ggtcgccagc ccgcgtacgt
     5161 gctgtatctc gagatggacc cggcgcgcgt ggacgtgaat gcgcacccgc agaagctcga
     5221 agtgcgtttc cgagagcctg gcctcgtgca cgacttcatc ttccgcacgc tggagcgcgc
     5281 cctcgcggaa acgcggccgg cgcagcctgc cgcgggtgcg atacccgccg cgcgcttcgg
     5341 tgcgcccccg ggggatgccg cccagcgcac gtccgcactc gagctgtaca cggctcttgg
     5401 cggcgtgcgc gacgaggcag gctcgacggg ccggggaggg acggccgggg tatcgcagtc
     5461 ggccgcgccg ccgcgaccgg gtgccgagct cccgaccgcg gaagtcccga ccgccgaaca
     5521 cccgctcggc tatgccatcg cgcaactgca cggcatctac atcctggcgc aggcgcaggg
     5581 cggactcgtg ctggtggaca tgcacgcggc gcacgagcgc acgacgtacg agcggctcaa
     5641 ggcttcgctc gagtcccggc gggtggcggc gcaaccgctg ctcgtcccga tcgtgctgaa
     5701 cgtctcgacg gccgaggccg acgaagccga ggaacatcac acgctattcg acgaactcgg
     5761 gatcgaaatg tcgcgctccg gcccggcgca gctcatcgtc cgatcgatgc cggcgctgct
     5821 gccgacggca gatcctgcgg tcctggtgcg cgacatgctc gccgacctgc gcgagcaagg
     5881 cagcggcgga gcccggcagg cgctcgatcg cgcgctggga accatggctt gtcaccaggc
     5941 cgtgcgcgcc aacaggcggc tcacgatccc cgagatgaac gcgctgctgc gcgagatgga
     6001 gacgacgttg cgcgccgacc agtgcgtgca cgggcgcccc acctggtcgt tcgtcggcat
     6061 ggacgacctc gaccggctgt tcatgcgcgg gcgttgatga cgacggctgc caggccggtc
     6121 gacgccgtgc tgctgatggg tccgacctgc agtggcaagt cgtcacttgc actgcaactc
     6181 gccgagcggt ttcccgtcga gatcgtcagc gtggactcgg cgcaggtcta ccgcgggatg
     6241 gacatcggga ccgcaaagcc cacttcggcg atccggtcct gggtgccgca tcacctggtc
     6301 gacgtctgtg acccgaccga aagctactct gccggtcgat ttcgccgcga cgcgctgcga
     6361 ctggtggcgg aaatccgggc gcgaggtcgg gtgcccctgc tcgtcggcgg cacgatgctc
     6421 tatttccgcg cgctcacgcg cgggatcgcg ccgctgcccg aggccagccc ggaggtccgc
     6481 gctcgcatcg acgagcgggc tcgccaacac ggttggcccg cgttgcacgc cgaactcgcc
     6541 gcgcatgatc cggccgcagc ggccagcatc cgcccggccg acggccagcg catccagcgg
     6601 gcactcgagg tcctggaact cacgggacgg cccctgtccg aactgcagcg gctggccgaa
     6661 ccttcgccgc tggccttcgc ctcgttcgtc ctgatgccgg tcgagcgagc ggtgttgtac
     6721 cggcgtatcg atcagcgctt cctcgacatg atgagcgaag gtttcctgaa cgaggtccgg
     6781 gcgctccggg cgcggggcga cctgcgcgcc gacctcccga gcctgcgggc cgtgggctac
     6841 cggcagctct gggctcacct cgagggtgac tgcggcctcg atacggcgat cgcggccggg
     6901 cagacggcca cccgcaacct cgccaagcgt caactcacgt gggtcaatgc ggatccatcc
     6961 ctatgctggc tgcggaccct tgaagatcag gaacttgcac ccatctacgg ggccatggcg
     7021 gcggcggccc cggccggcgc gccgtagggc tatgttagga ttgggttcgt tcttgcgcgc
     7081 cgaggcatcg agcctcacgg ccgctcacgg aagagtcagc ggcaggaacg agcggattga
     7141 acaataagga gacgcgatca tggccaaggg ccagtccctg caggacccct tcctcaacgc
     7201 tctgcgcaag gagcgtatcc ccgtgtcgat ctacctggtc aacggcatca agctgcaggg
     7261 ccagatcgat tccttcgacc agttcgtcgt gttgctccgc aacagcgtga gccagatggt
     7321 gtacaagcac gcgatttcca cgatcgtgcc ggcccgcaac gtgcggctgc cgatcggcga
     7381 cgaggaactg caggcggagg acgcgccggc ggcgtgagcc cggcgcccgg cagggcggag
     7441 tcaggacccc ttgttcgaca ggccgggcaa cggcgaacgc gcgatcctcg tctgcgtcgg
     7501 aatcggccac gcgccggaac cggatcgcat ggaggagttc cgcgcactgg cgcggtccgc
     7561 gggcgccgag gtgctcgagg tcctgaccgc cacgcgcaag gcccctgaac cgcgcttctt
     7621 cctcggcacc ggcaaggtcg ccgagctgcg cgagcgcctg gtcgcgctcg gcgccgacct
     7681 cgtggtggtg gatcacgcgc tcagcccggg gcaggagcgc aacctcgaga aggaactcac
     7741 atgccgcgtg ctcgaccgca gcggcctcat cctggacatc ttcgcccagc gcgcccgcac
     7801 cttcgaaggc aagctccagg tggaactcgc gcaactcgag cacatgtcca cgcggctcgt
     7861 gcgcgggtgg acccacctcg agcgacagaa gggcggcatc ggattgcgcg gcccgggcga
     7921 gacccagctc gaaaccgatc gccgcctgat cgggcgtcgc atcaagacac tgaacgaccg
     7981 gctcgacaag gtcctggtac aacgcgagac cacccggcag gagcgccggc gtggcgcggt
     8041 gccgaccgtc gcgctggtcg ggtacacgaa cgcgggcaag tcgacgctat tcaatcgtct
     8101 cacttctgcc ggggcctacg ccgctgacca gctgtttgcg acgctggacc cgaccgtgcg
     8161 ccgcctgcgg ctcgcaccgg gcctcgacgt ggcgctcgcc gataccgtcg gattcgtgcg
     8221 cgacctgccg cacgagctgg tcgccgcttt ccgctcgacg ctgcaggagg cccgcgaggc
     8281 cgacctgctg ctgcacgtga tcgacgccgc cgaccccgag cgcgccgagc gcgtcacgca
     8341 ggtcaacgag gtcctcgagt cgatcggagc gcagtcgctg ccccagatcg cggtgttcaa
     8401 caagatcgac cgctccggcg aggcgccgca cgtcgatcgc gactcggatg gccgcgtgga
     8461 ccgcatctgg ctgtcggcgc acagcggcgc cggggtggac ctgttgcgcg acgcgctgca
     8521 ccgacagctc gggtcggacc ttcaccattg cgtcctgcgc gtgccgccgc acgccggccg
     8581 cgcgcgggcc cagctgttcg aggccggcgc ggtgctgtcg cagcggccgc tcgacggcgg
     8641 tgacatcgaa ctcgaggtca gcctgcgccg gcgggacctc gaccggatct gccgggaggc
     8701 cggcctcgac gtcccgcccg cgatcgagcc tccttgtgcg cccgacgagg cgttcctaca
     8761 atcccgtggt tccgccgacc gagacgccgc cgtagcggtg gccacggcgg agccggacgt
     8821 acccctcacc gaccgaccgc tggcaatcgg gagtaactga agtcccatgg cttggaatga
     8881 cccgggcagg aatcgcaatc cctggggcaa tcgccccgac aagggagccg ccgacctcga
     8941 cgaggtcctg cgcaacctgc agaaaaaact ggctggcctg ttcggtggcg gaggcggcgc
     9001 gggcggcgag tccggcggcg gcgacgccgc gatgcgcggc ttcggcgtga gcaccgtggt
     9061 ggtcgtgctg ctgctcgtct gggcggcgac cggcctgtac aaggtggacg ccgccgagcg
     9121 cggcgtcgtg acccgcttcg gcaagtacgt cgagaccacc gagcccgggc tgcgctggca
     9181 cctgccgtgg ccgatcgagt cgcggcagat cgtcaacgtc gagaccgtcg agggcatgga
     9241 cgaccagacg cgaatgctga cctccgacga gaacctcgtc gacatcaatt tcgcggtcca
     9301 gtaccggcgc gccgagccgg tcgcctacgc gttcagcgtg cgcgacccgc aggaaacgct
     9361 cggggaagtg agcgagagcg cgatccgcga gatcgtcggc cagagcacgc tcgacttcgt
     9421 gctcgaggcg gggcgccagg agatcaccgc caagacgcgc gaactgatcc agcgcacgct
     9481 cgactcgtac aagaccggca tcgaggtcac gaccgtgaac ctgcagggcg tgagcgtgcc
     9541 ggagcaggtg cagagctcgc agcgcgacgc gatcaaggcg cgcgaggaca aggagcgcct
     9601 cgcgctcgag gcgcaggcct actcgaacga catcctgccc aaggccaagg gctccgccgc
     9661 acgcctggcc gcggattccc aggcctacaa gtcgcgcatc gtcgcggatg ccgagggtga
     9721 agcgtcgcgc ttctcgcagc tggcggaagc gtatcggcgg gcaccggcag tcactcgcaa
     9781 gcgtctctac ctggagacga tggaggaggt cctcgggcac tccaacacgg tgctggtgga
     9841 taccaagggc acgggcaaca tgatctacct gccgctcgac aagctgaccg aacggcgcgc
     9901 cccggtgcag acgctcgacc agcagcgcca ggcgacctcc gcgtcgccgc aggcgccggc
     9961 ggaggagccg gccaccacgg tcgagtcgcg ccgcgagcgg gggacacgct gatgggcagc
    10021 cgcagctacc tgggcgtcat cctggccggc ttcgccgcgc tcctggtgtc gatgtccgcg
    10081 ttcaccgtcc gcgagacgga cctcgccatc aagttccgct tcggcgagat cgtgaatgcg
    10141 aactactcgc cgggcctgca cttcatgacg ccgctggtga acaacgtgcg caagttcgac
    10201 aagcgcatcc tcaccgagga ctacccggcc gagcagttcc tcaccagcga aggcaagatc
    10261 ctcaagatca acttcttcgt gaagtggcgc atcagcgacg tgtcgcgcta ctacatctcg
    10321 acgtccggcg gccaggaaga ggtcgcggcg cggcgactcg gggaaatcgt caaggacggc
    10381 ctgaagggcg tgatcgcgcg ccggaccatc cagcaggtcg tcgcggccga gcgcagcgag
    10441 ttcctcggcg aggtgctcaa gatcgccggc gccaacgtcg gcggcctcgg catgaccatg
    10501 atcgacgtgc gcgtgaagag catcgagctg cccgaggagg tcagcgagtc ggtgttcaac
    10561 cgcatgcgcc aggacttcgc gcgccaggcg gcgcagttgc gcgccgaggg ctcggcgatc
    10621 tccgagcaga ctcgcgccga ggcggaccgg cagcgcaccg agatcctcgc caacgcctat
    10681 cgcgacgcgg agaaggtgaa aggcgagggc gacgccaccg cggccgacat ctacgcgaag
    10741 gcgtacggtc gcgagccgga tttctacgag ttcttccgct cgctgcaggc ctaccgcaag
    10801 tcgctcggca agcagggcga cgtgctcgtg ctcgcgccgg acggcgagtt cttcaagtac
    10861 ctgaaggagg ccggccgctg agcgcggccg gccggcagtc ccgatgcagt ggggcgacct
    10921 ctgggcggcc ttcgccctgt acctcgtgct cgagggactc atgccgttcc tgaatcccgg
    10981 cggcatgaag cgcacgctgg cgaaactctc ggacatgccg gaaggcacgc tgcgggcggc
    11041 gggcctcggc agcatgatcg ccggcgcgat cctcctctat tacgtgcgcg gcggcgcgtg
    11101 aatcaactcc ggacggacgg gctcatggca aggaacctgg tggtcatcgg cgcccagtgg
    11161 ggcgacgagg gcaagggcaa gatcgtggac ctgctcaccg agcgggcggc ggcggtggtg
    11221 cgcttccagg gcgggcacaa cgcagggcac acgctggtca tcgaaggcgt gaagacggtg
    11281 ctgtcgctga tcccgtcggg catcctgcat cccggcgtgc agtgcctgat cggcaatggc
    11341 gtggtgctgt cgctcgaggc gctgctgcgc gaggccgatg cgctggtgca gaagggcgtc
    11401 ccggtgtacg accggctgcg cgtgagcccg agctgcgcgc tgatcctgcc ttcgcacatc
    11461 gcgatcgacc gcgcgcgcga gcgcgccaag ggcgccaacg cgatcggcac gaccggccgc
    11521 ggcatcggcc cggcctacga ggacaaggtc ggccggcgcg cggtgcgggt ggccgacctc
    11581 ttccagcgcg atcgcctcgc ggcgcgcct
//
LOCUS       DAOMFD010000055        11482 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1482_length_11482_cov_26.427409, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000055 DAOMFD010000000
VERSION     DAOMFD010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11482)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11482
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1482_length_11482_cov_26.427409"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="PL152_04940"
     CDS             <1..950
                     /locus_tag="PL152_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48659.1"
                     /translation="VGALKLKLAPEEKKAIGQRGTTNLDAYNLYLMARQHYASSFSSD
                     ARKSEAVIRLCRGAIELDPHYARAWALMATAQNNRLYATGDGDSGAEAATRAIALDRN
                     LAEAHAARAGILAANGEYAAGQEEIAIALRLDPDSVDVNREAARLLFHQRRFGDAIAH
                     WEKVSSLVEADYASGPLLLTCYTALGDQDSVLRVARRTLARAEAAARAEPDNGMAMGA
                     VVSCLVALGEMDRAREWSRRAVLMDPDNMSMRYNLACDLVTHLQDFDMALEMLRPVCA
                     RMGRERLEWLRSDPDLGPIRGDPRFAAMIEAADRRLAGS"
     gene            988..2838
                     /locus_tag="PL152_04945"
     CDS             988..2838
                     /locus_tag="PL152_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48660.1"
                     /translation="MGDEPGDGFWARLRRRKVVQWGIAYAAGAWGLLQGLNYVSAIFH
                     WPEYLQRVAALALLIGLPVVLVLAWYHGDQGRQRVTGPELAIISLLFLLGGGLFWRYD
                     RVTPPPAPAGETAASAGAGTVTPGGGAASSSDQAPANSIAVLPFVNMSGDPANEYFSD
                     GISEEILNVLARAPELQVAARTSSFTFKGKNAEVPEIARELKVRMVLEGSVRKQSDKV
                     RITAQLIDAKTGFHVWSETYDRELKDIFAIQDEIARAIGDKMKVQVVGAGPGSAAVPR
                     TTRPEAHDHYLRGLALWQTRNEANLWQAIDEFNKAITIDHKYAEAYGGLALAYTVIAD
                     YSGRITNEEAAAKTAAAAEMALALDPSMPEPYAALGNMAANGRARVLGEALLRRAIAL
                     RPSFATANQWLGTIIMSGGDAASGLEFSERSSTLDPRSPIVADNHAVVLIALGRYEDA
                     KSSCQRIQTFAPDFIPCLIEIGISDIMLGKIEDARHSLVRAAELRNPSAVPVTNQLID
                     ALSGRGDKHALAVRLASFSPRSFLEQGSGNAFDLQDVPILLIVLGEPQVALDYIQRHA
                     GEPYGTLDYGMVRAALDPIRCDPRFQAAAKKLAINDVRAAKLCAPAKPAR"
     gene            complement(2848..3336)
                     /locus_tag="PL152_04950"
     CDS             complement(2848..3336)
                     /locus_tag="PL152_04950"
                     /inference="COORDINATES: protein motif:HMM:NF013098.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HQR48661.1"
                     /translation="MIRKLHHAAYRCRDSEETRRFYEDFLGLPLAGTLEIRETKTGRE
                     TDVLHTFYRLDDGSHLAFFEAPDQPFEFKAQHDFDLHIALEVGAQDVERMLAKGKAAG
                     METRGVSDHGFIHSIYFRDPNGYVVELCAKTPGHDAAMDPARNRAREMLDRWTAEKAR
                     GA"
     gene            complement(3366..4043)
                     /locus_tag="PL152_04955"
     CDS             complement(3366..4043)
                     /locus_tag="PL152_04955"
                     /inference="COORDINATES: protein motif:HMM:NF024809.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR48662.1"
                     /translation="MRLYYHPVSTACRPVMLLAAEDGIDLEYKLVDLFKGEQLQPEFA
                     AINPSRLVPALDDDGFILTEGSAILKYLADKAGSTAYPKELRQRARVNELMDWFNTGL
                     YRDLGYGLVYPQTLPHHKRPDARVQAGVLEWGREKSRHWLGILDQHLLGPKGAWLAGN
                     AVSLADYFGAAIVTLGEVIRLDYGAWPNVTRWLCNVKARPAWSKVNEAFYAHFVAPFK
                     DAQFVGL"
     gene            4201..5979
                     /locus_tag="PL152_04960"
     CDS             4201..5979
                     /locus_tag="PL152_04960"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HQR48663.1"
                     /translation="MAEIFVSYSRQDKARVAPLVSALEAEGWSVWWDPEITPGDEFDS
                     LISRELDAARAVIVVWTGTSVDSRWVRGEARDAADRGALVPVRFDNARMPIDFRAVHT
                     TDLDGWGEDPQHESFRALCRALEAKLGSKKKKAAGTAIAVLPLANMSADPENEYFSDG
                     MTEEIINALSKVPGIHVASRSSSFAFKGRKDVDARQIGEKLGVTTLLEGSVRKVGNRI
                     RIAAQLVNVENGYQLWSETYDRQLEDIFALQDEISRSIVDALKLRLGADEAAVVAPTK
                     SLDAYTTYLKGRFHFNKFSEAGLKKALDLFQGALLQDPGFARAYAGIADVWCDLADDW
                     VPPDEAYPRAKSAAERALQRDPELADAMISIGKVLCWHEWKFADGVKQVERAVALSPN
                     NAEAHWVLGTALPLVGRLHEGYKSVRKALELDPLRVEYFGWATRLLLYAGDYEAALKQ
                     GEETFEIDENYGRGFIFVGSTHLAMGHADTALDWFQRGQRLDTAVRSYDAMIVRALAA
                     LGRREEAEEIMARLEAESRQKYVRAEYLAMGHGALGQFDKAFEALERAYQARSAGLIY
                     LHLDPGYEPLRGDPRYHELVQRMGLR"
     gene            6026..7855
                     /locus_tag="PL152_04965"
     CDS             6026..7855
                     /locus_tag="PL152_04965"
                     /inference="COORDINATES: protein motif:HMM:NF024820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR48664.1"
                     /translation="MTEANVETLWQRLRRRKVVQWGIAYAAGAWGLLQGLGYVTQTFS
                     WSPLVPQLATLALLVGLPIVLVLAWYHGDRGEQRVTATELAIIALLFLIGGALFWRYE
                     RIASARRPATAANVESGIDTTASAVGKAPRNSIAVLPFVNMSQDTANEYFSDGISEEI
                     LNSLAQIPALKVAARTSSFSFKGEKKEVPDIARDLKVRMVLEGSVRKQGERVRITAQL
                     IDAENGFHVWSQTYDRDLKDIFAVQDEIAHAIAGALRMKLAATHAASNGKPGSVDVQA
                     YDLYLRGLALWQARGKDNIRAALSCFKQATARDPKLAKAWAGIALVETISPDWFGTPE
                     DEAARATRDAAERALALDPTLPEAYAALGNTAFNDGRWDTGRALFDRAIALSPSYATA
                     WQWYGIGLSMVDTKGEAVAIAKRAVELDPKSSVVRQDLAMTLYRAGRFEESEAESRRL
                     LDDPQVRETNLTIVFDNQMARRDYAGARETMLRAAEPRGPASVAFVNQAMDAFEGKAD
                     PAPIARRLLAAKDGFLFPESVSPFQEPDLLLWFIAAGRTDDAVARLGGLAAWFPYYAR
                     QQIADPNLDSIRCRPDFRDIVAKVGSGDPRAEKVCGTDAIAAR"
     gene            complement(7882..9138)
                     /locus_tag="PL152_04970"
     CDS             complement(7882..9138)
                     /locus_tag="PL152_04970"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR48665.1"
                     /translation="MPGATVAESRAGWPRYYTVVLLLCAAVFISYIDRTNISVAAIAM
                     KDELGWTETDKGTVLSSFFVGYLLMMAASGALANRYGGWLVLGIAVLWWSAWTILTPP
                     AATVSLAALVAARIGLGLGEAAVFPASINMIGRWVPPERRSRATALLVSALSLGTLFS
                     LPVTGWMVRAHGWPVPFYVFGAAGLVWFVAWFALIGEHRAAAPAHVAETAASEARHIP
                     WGRLLALPAVWAIIVAHFACNWGLYLTLAWMPSYFRSTFGVSLADAGLLSAAPWLVSF
                     VTANLVGAWADRMLKAGRKAGFVRKLLQGISLVGGGLVWLLLPHATTPAVGVALMCCA
                     TCVGAFAMSGFAPNCFDIAPKYADVIWGISNSVATVPGIVAVFITGWLVDRTGNFAAP
                     FNLTAAILLGSAVVYQLFGSGERLVD"
     gene            9150..9395
                     /locus_tag="PL152_04975"
     CDS             9150..9395
                     /locus_tag="PL152_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48666.1"
                     /translation="MFVGRSYPTSGPASPQVDSGAARVDRAARLSGARQLQLGPTATI
                     KTQWEEIIIERSGGKQDTSLVVVSSTVMGITVKVHED"
     gene            complement(9406..9894)
                     /locus_tag="PL152_04980"
     CDS             complement(9406..9894)
                     /locus_tag="PL152_04980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48667.1"
                     /translation="MLATGCHSDRTPDGAAQERRSEVGIEFGIWEPGPDGDPRFIATL
                     EVPRTADQAYGWRLRARDPNQPVKWVETLRLPAPAQSWDGVDENPNVTVSEDGRTATT
                     YGQSEPGDSYIGNVWGVSSDDPLGEYQLTVEFEDGRKETITFHVVPGKDGDDDNGPGV
                     MI"
     gene            complement(10038..10772)
                     /locus_tag="PL152_04985"
     CDS             complement(10038..10772)
                     /locus_tag="PL152_04985"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HQR48668.1"
                     /translation="MRVIDGDTIDVRLQSGPIRVRLHSIDAPEHDQPWGPQAKAALAS
                     RVDGRIVALDVVTQDRYDRLVAEVLLGSENVNAWLVQQGDAWAYRDYMDDASFCAYEG
                     IARASRLGLWSLPPGTWVAPWEWRAIEKGRAASRTDYSHETTANCILAMQASAGFSRD
                     LHSVESAGSSDMKRGPGQQTVPAPGPLPPNDHCLIKGNIGSNGRIYHVPGSASYDATK
                     IDVSKGERWFCTEAEARAAGWRPAKN"
     gene            complement(11009..>11482)
                     /locus_tag="PL152_04990"
     CDS             complement(11009..>11482)
                     /locus_tag="PL152_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48669.1"
                     /translation="IAEIAGVFIGFGALISAAGEQSKDARNKLVSVAFIGITVLVSAI
                     LPVWLARFGLGGAALWEWSSAGFLALIWATILIAFSDGDFRRWHLEDAKANLPRAVLF
                     WVFLEIPIQVSLVLAILGVAPSLAPAFYVTALVLNLFEAAMMLARHILARPASAA"
BASE COUNT         1994 a         3891 c         3837 g         1760 t
ORIGIN      
        1 tcgtcggcgc gctgaagttg aagctcgcac ccgaggagaa gaaggccatc gggcaacggg
       61 gcacgaccaa cctcgatgcc tacaacctgt acctgatggc gaggcagcac tacgccagca
      121 gcttttccag cgacgcccgg aagagcgaag ccgtcatacg cctgtgccgg ggcgccatcg
      181 agctcgatcc tcactatgcg cgtgcctggg cgctgatggc gaccgcgcag aataaccggc
      241 tgtacgcgac gggtgacggg gacagtgggg ccgaagcggc gacgcgcgcc attgcgctcg
      301 accggaacct ggcggaggcc catgccgccc gggccggcat cctcgcggcc aatggcgagt
      361 acgcggcggg ccaggaggag atcgcgatcg ccctgcgtct cgacccggac tccgtcgatg
      421 tcaatcggga ggcggcgcgc ctcctcttcc accagcgccg cttcggggac gccattgcgc
      481 actgggagaa ggtctcttcg ctggtcgagg ccgactacgc gtcgggtccg ctgctgctca
      541 cgtgctacac ggcgctcggc gaccaggaca gcgttctgcg tgtcgcccgg cgcacgctcg
      601 cccgcgccga ggccgccgcc cgcgccgagc ccgacaacgg catggccatg ggtgccgtcg
      661 tttcctgcct ggttgcgctc ggcgagatgg atcgggcgcg cgagtggtcc cggcgtgctg
      721 tactcatgga cccggacaac atgtccatgc gctacaacct ggcctgcgac ctcgtgacgc
      781 acctgcagga tttcgacatg gccctcgaga tgctgcgccc ggtctgcgcg cggatgggcc
      841 gagagcgact cgaatggctg cggagcgacc cggacctcgg cccgatccgc ggcgacccgc
      901 gtttcgcggc catgatcgag gctgccgatc ggcggcttgc cggctcctga gcctcgatcg
      961 cagccactgg cggcaaggtg aagaccattg ggtgacgagc cgggcgacgg attctgggca
     1021 cgcctgcgcc gacgcaaggt cgtgcagtgg ggcatcgcgt acgccgcggg tgcgtgggga
     1081 ctgctgcagg gcctgaacta cgtcagcgcg atcttccact ggccggagta cctgcaacgg
     1141 gtcgccgcgc tcgcacttct catcggcctc cctgtcgtcc tggtgttggc gtggtatcac
     1201 ggggaccagg gacggcagcg cgtcaccggg ccggaactcg cgatcatctc cctgctgttc
     1261 ctgctggggg gcggactgtt ctggcgctat gaccgcgtca ccccgccacc ggccccggcg
     1321 ggcgaaaccg ccgcgagcgc aggcgccggc accgtgacgc cagggggagg agcggcgtcc
     1381 agctcagacc aggcgccggc gaactccatc gcggtgctgc cgttcgtcaa catgtcgggc
     1441 gacccggcga acgagtactt ctccgacggc atcagcgagg agatcctgaa cgtgctcgca
     1501 cgcgcgcccg aactccaggt cgcggctcgc acctcgtcgt tcaccttcaa gggcaagaac
     1561 gcggaagtcc ccgagatcgc gcgcgagctc aaggtgcgca tggtgctcga gggcagcgtc
     1621 cgcaagcaga gcgacaaggt gcgcatcacg gcacagctga tcgacgcgaa gaccggattt
     1681 cacgtctggt cggagaccta cgaccgcgag cttaaagaca tcttcgcgat tcaggacgag
     1741 atcgcgcgtg ccatcggcga caagatgaag gtgcaggtcg ttggtgccgg accgggcagc
     1801 gcggccgtcc cgcgcaccac gaggcccgag gcgcacgatc actacctgcg cggacttgcg
     1861 ctctggcaga cgcggaacga agccaatttg tggcaggcga tcgacgagtt caacaaggcc
     1921 attacgatcg atcacaagta cgcggaggct tacggcggac tcgctctcgc gtacaccgtc
     1981 atcgcggact actcgggaag aatcaccaac gaggaagcgg cggccaagac ggccgcggcg
     2041 gcggaaatgg cgctcgcgct ggacccgtcg atgccggagc cctatgcggc tctcggcaat
     2101 atggctgcaa atggccgggc ccgtgtcctc ggtgaagcgc tgctgcggcg cgcaattgcg
     2161 ttgcggcctt ccttcgcgac cgccaatcaa tggctgggaa cgatcatcat gtccggcggc
     2221 gacgccgcga gcggtctcga gttctccgaa cgctcgagca ccctcgaccc gcgctcaccc
     2281 atcgtcgcgg acaaccacgc ggtcgtgctg atcgccctcg ggcgttacga ggatgcaaag
     2341 agttcctgtc agcgtatcca gacgtttgct ccggacttca ttccgtgcct gatcgagatc
     2401 ggcatctcgg acatcatgct cggcaagatc gaagacgccc ggcattccct cgtcagggca
     2461 gctgaactac gcaatccgag tgccgtcccg gtgacgaatc agctgatcga cgcactctct
     2521 ggtcgcggcg acaagcacgc gctggctgtg cgcctggcga gcttttcgcc acgcagcttc
     2581 ctcgaacaag gcagcggcaa cgcctttgac ctgcaggacg tcccaatcct gctcatcgtg
     2641 ctcggcgaac cgcaagtcgc gctcgactac atccagcgcc atgcgggcga accttatggc
     2701 acgctcgact atggcatggt ccgcgcggcg ctggacccga tccgctgcga cccgcggttc
     2761 caggccgcgg cgaagaagct cgccatcaac gacgtacgcg ccgcgaagct gtgcgcgccg
     2821 gcgaaacccg cgcgctgagc gccgcagtca ggcgccgcgc gccttctcgg ccgtccaccg
     2881 atcgagcatc tcacgcgcac gattgcgcgc ggggtccatg gctgcatcgt gtccgggtgt
     2941 cttcgcgcag agttccacca cgtagccgtt cgggtcgcgg aagtagatcg agtggatgaa
     3001 accgtgatca ctcacgccac gcgtctccat cccggccgcc tttcccttcg cgagcatccg
     3061 ttcgacgtcc tgggcaccga cctccagcgc gatgtgcagg tcgaagtcgt gttgcgcctt
     3121 gaactcgaac ggctggtcgg gcgcttcgaa gaatgccagg tgcgatccgt cgtccagccg
     3181 gtagaacgtg tgcagcacgt ccgtttcacg acccgtcttg gtctcccgga tctcgagcgt
     3241 cccggcgagt ggcagcccca ggaagtcctc gtagaacctc cgggtctcct cggaatcgcg
     3301 gcatcggtac gccgcgtgat gcagcttgcg gatcattgcc atcttcctcc gcgcattgcc
     3361 cggcgtcaga gcccgacgaa ctgcgcgtcc ttgaacggtg cgacgaagtg cgcatagaag
     3421 gcttcgttca ccttcgacca tgcggggcgt gccttcacgt tgcagagcca tcgagtgacg
     3481 ttgggccatg cgccgtaatc gagccggatc acctcgccga gcgtcacgat cgcggcaccg
     3541 aaatagtccg cgagactcac cgcgttgccc gcgagccagg cccccttggg cccgagcaga
     3601 tgctgatcga gaattccgag ccagtggcgt gacttctcgc ggccccactc gaggaccccc
     3661 gcctgcacgc gtgcgtccgg tcgcttgtgg tgcggcaggg tctgaggata gacgagcccg
     3721 tagccaaggt cgcgatacaa gccggtattg aaccagtcca tcagttcgtt gacccgcgcg
     3781 cgctgccgga gttccttcgg gtaggccgtc gagccggcct tgtcggcgag gtacttcagg
     3841 atcgccgacc cctccgtgag gatgaatcca tcgtcgtcga gcgccgggac caggcggctc
     3901 gggttgatcg cggcgaactc cggctgcaac tgctcaccct tgaacaggtc gacgagcttg
     3961 tactcgagat cgatgccgtc ctccgcggcg agcagcatga ccggacggca ggcggtggag
     4021 accggatggt agtagagccg catggcgtga gtcctgaggc ggatcgaggt cggacatcct
     4081 actcgcttgc cgcgccggcg gcggtggagc cgcgcggcga cactcgggca tcgcgcccgg
     4141 accgccggcc gcctcggcgg gctgtgctag agtcagctcc cggcaggcgg gagaaccttt
     4201 ttggccgaga tattcgtctc atactcgagg caggacaaag cgcgcgtcgc tccgctcgta
     4261 tcggcgctcg aggccgaggg ctggagcgtc tggtgggacc cggagatcac gccgggcgac
     4321 gagttcgact cgctgatctc ccgtgagctg gatgccgcgc gcgccgtcat cgtcgtgtgg
     4381 acgggcacct cggtcgactc acgctgggtt cgtggcgagg cgcgtgacgc cgccgatcgc
     4441 ggagcgctgg tgcccgtgcg tttcgacaac gcccgcatgc cgatcgactt ccgcgccgtg
     4501 cacaccaccg atctcgacgg ctggggcgag gatccgcagc acgagtcgtt ccgcgcgctg
     4561 tgcagggcgc tcgaggcgaa gctcggctcg aagaagaaga aggcggcggg tacggcgatt
     4621 gcggtccttc cgctcgccaa catgagcgcc gacccggaaa acgagtactt cagcgacggg
     4681 atgaccgagg agatcatcaa cgcgctgtcc aaggtgccgg gtatccacgt cgcatcgcgc
     4741 tcgtcgtcgt tcgcgttcaa gggtcgcaag gacgtggatg cccgccagat cggcgagaag
     4801 ctcggcgtga cgacgctgct cgaaggcagc gtgcgcaagg tcggcaaccg aatccgcatc
     4861 gccgcgcagc tggtcaacgt cgagaacggc taccagctct ggtcggagac gtacgaccgc
     4921 cagctcgagg acatcttcgc gctgcaggac gagatctcgc gctcgatcgt cgatgcactg
     4981 aagctgagac tcggcgccga cgaagccgcg gtcgtcgcgc cgaccaagag cctcgacgcc
     5041 tacacgacgt acctcaaggg ccggttccac ttcaacaagt tcagcgaggc cggcctcaag
     5101 aaggcgctcg acctgttcca gggcgcgctg ctgcaggatc ccggctttgc acgcgcatat
     5161 gcgggcatcg ccgacgtgtg gtgcgatctc gccgacgact gggtaccgcc cgacgaagcg
     5221 tacccgcgcg cgaagtcggc ggccgagcgc gcgctgcaga gggacccgga actcgccgac
     5281 gcgatgatct cgatcggcaa ggtcctgtgc tggcacgaat ggaagttcgc ggatggcgtg
     5341 aagcaggtcg agcgcgccgt cgccctgagc cccaacaatg ccgaagccca ctgggtactg
     5401 ggcacggccc tgccgctcgt gggtcgcctc cacgagggtt acaagtcggt gcgcaaggct
     5461 ctcgagctcg atcccctgcg cgtcgagtac ttcggctggg ccacgcgcct cctgctctac
     5521 gccggggact acgaggccgc gctcaagcag ggcgaggaga ccttcgagat cgacgagaac
     5581 tacggccgcg gcttcatctt cgtcggctcg acacatctcg cgatgggaca tgccgacacc
     5641 gcgctcgact ggttccagcg cggccagcgt ctcgacaccg cagtgcgctc gtacgacgcg
     5701 atgatcgtac gcgcgctggc ggcactcggc cgacgcgagg aagccgagga aatcatggcc
     5761 cgcctcgagg ccgagtcgcg acagaaatac gtgcgcgccg agtaccttgc catgggtcat
     5821 ggcgcgctgg ggcagttcga caaggccttc gaagcgcttg agcgggcgta ccaggcgcgg
     5881 tcggccggcc tcatctacct gcatctcgac ccgggctacg agccgctgcg cggcgacccg
     5941 cgttaccacg agctggtgca gcgcatgggc ctgcgctagg cgcgccggcg tcgcgagtcc
     6001 agggacacca ggacggatcc gacggatgac ggaagcgaac gtcgagacgc tctggcaacg
     6061 acttcggcgc cgcaaggtcg tgcagtgggg aattgcctac gccgccggag cgtggggcct
     6121 gctgcagggc ctcggctacg tcacgcagac gttcagttgg tcgccgctcg tcccgcagtt
     6181 ggccaccctc gcgctgctcg tcggcctccc gatcgtgctg gtgctcgcct ggtatcacgg
     6241 cgaccgcggc gagcaacggg tcaccgcgac ggagctggcg atcatcgcac tgctgttcct
     6301 catcggcgga gccctgttct ggcgttacga acgtatcgcc tcggcccgcc gccctgccac
     6361 tgccgcgaac gtcgagagcg gcatcgatac taccgccagc gcagtcggca aggcaccgcg
     6421 caactccatc gcggtgctgc cgttcgtgaa catgagccag gacacggcta acgagtactt
     6481 ctcggacggc atctccgagg agatcctgaa ctcgctcgcg cagatcccgg ccctgaaggt
     6541 ggccgcgcgc acttcgtcgt tctccttcaa gggcgagaag aaggaagtcc cggacattgc
     6601 gcgcgacctc aaggtgcgca tggtgctcga gggcagcgtg cgcaagcagg gcgaacgcgt
     6661 gcgcatcacg gcgcagctca tcgacgcgga gaacggcttt cacgtctggt cgcagaccta
     6721 cgaccgcgac ctcaaggaca tctttgcagt ccaggacgag atcgcgcacg ccatcgcagg
     6781 cgcactgcgg atgaagctgg ctgcgacgca tgcggcgagc aacgggaaac ccgggtccgt
     6841 ggacgtgcag gcgtacgacc tgtacctccg gggcctggcg ctgtggcagg cgcgtggcaa
     6901 ggacaacatc cgggcggcgc tcagctgttt caagcaggcg accgcgcgtg acccgaagct
     6961 cgccaaggcg tgggcgggca ttgcgctcgt cgagaccatc tcgcccgact ggttcggcac
     7021 accggaggac gaggctgcgc gcgccacgcg cgacgcggcg gaacgcgccc tggccctgga
     7081 tccgaccctt cccgaagcct acgcggcgct cggcaatacg gcattcaacg acggacgctg
     7141 ggacacgggc cgcgccctgt tcgatcgcgc catcgcgctg tcgccttcgt acgcgaccgc
     7201 atggcagtgg tacggcatcg ggctgtcgat ggtcgacacg aagggggaag ccgtggccat
     7261 cgcgaagcgg gccgtcgagc tggaccccaa gtcctccgtg gtccgccagg acctggcgat
     7321 gacgctgtac cgggccggtc gcttcgagga gtcggaggcg gagagccgca ggctcctgga
     7381 cgatccgcag gtgcgcgaaa ccaacctgac gatcgtcttc gacaaccaga tggcgcgcag
     7441 ggactacgcc ggggcgcgcg agacgatgct gcgggccgcg gagccgcgcg ggccggcatc
     7501 ggtggcgttc gtcaaccagg ccatggacgc gttcgagggc aaggcggatc cggcgccgat
     7561 cgctcggcga ctgctcgctg cgaaagacgg attcctgttt ccggaatcgg tctcgccgtt
     7621 ccaggaaccg gatctcctgc tgtggttcat cgccgccggc cggactgacg acgccgtcgc
     7681 gcgtctcggt ggcctcgcgg cgtggttccc ctactacgcg cgtcagcaga tcgccgaccc
     7741 caacctggac tcgatccgct gccgtccgga tttccgtgac atcgtcgcga aggtgggctc
     7801 gggcgacccc cgtgccgaga aggtctgcgg cacggacgcg atcgcggctc gttgagtgag
     7861 aaccgcgcgt tccgctacgc cctagtcgac gagccgctcg ccgctgccga acagctgata
     7921 gacgaccgcc gacccgagca ggatcgccgc cgtcaggttg aacggcgccg cgaaatttcc
     7981 cgtccggtcg acgagccaac cggtgatgaa cacggcgacg atgcccggca ccgtggccac
     8041 gctgttggag atgccccaga tcacgtccgc gtacttcggc gcgatgtcga aacagttcgg
     8101 tgcgaatccc gacatcgcaa atgcgccgac gcaggtcgcg cagcacatga gcgcaactcc
     8161 gaccgcgggc gtcgtcgcgt gcggcagcag caaccacacc aggccgcccc cgacgagcga
     8221 gatcccctgc aggagcttgc gcacgaaacc ggctttgcgc cctgccttga gcatgcgatc
     8281 ggcccaggca cccacgaggt tggccgtgac gaagctcacc agccacgggg cggcggacag
     8341 caatcctgca tcggcaagcg agacgccgaa tgtcgaccgg aaatagctcg gcatccacgc
     8401 gagcgtgagg tacaggcccc agttgcaggc gaagtgcgcg acgatgatcg cccacaccgc
     8461 aggcagcgca agcaggcgtc cccagggtat gtggcgtgct tccgaggcgg cggtttcggc
     8521 aacatgcgcc ggcgcagccg cacggtgctc gccgatgagc gcgaaccacg cgacgaacca
     8581 cacgagcccg gccgcgccga acacgtagaa cggcaccggc cagccgtggg cccgcaccat
     8641 ccagccggtc accgggagcg agaacagcgt gccgagcgac agcgcactca ccagcagcgc
     8701 cgtagccctc gagcgacgct ccggcggtac ccagcgaccg atcatgttga tggacgccgg
     8761 gaacaccgcg gcctcaccca ggccgagccc gatccgcgct gcaacaagcg cggcgagcga
     8821 aaccgtcgcc gccggcggcg tgaggatcgt ccaggccgac caccacagca cggcgatacc
     8881 gagcacgagc cagcctccgt accggttcgc cagcgcgccg ctcgccgcca tcatgagcag
     8941 gtagccgacg aagaacgacg acagcaccgt ccccttgtcg gtctcggtcc agccgagttc
     9001 gtccttcatc gcgatggccg cgacggagat gttggtgcgg tcgatgtacg agatgaagac
     9061 cgcggcgcag agcagcagca ccacggtgta gtaccgcggc cagcccgcac ggctttctgc
     9121 gaccgttgcg ccaggcatgg aatccccctt tgttcgtcgg gcgatcctac ccgacatcgg
     9181 ggccggcgtc gccgcaagtc gattcaggcg ctgcaagggt cgatcgggcg gcgcgactat
     9241 ccggcgcaag acagcttcaa ctaggcccga cggcaaccat caaaacgcaa tgggaagaga
     9301 tcatcatcga gcgcagcggt ggcaagcagg acacgtcgct cgtcgtggtg agctcgacgg
     9361 tgatgggcat caccgtgaag gtgcacgagg actgacccac ccggctcaga tcatcacgcc
     9421 cggcccgttg tcgtcgtcac cgtccttgcc cggcacgacg tggaacgtga tcgtctcctt
     9481 gcggccgtcc tcgaactcga cggtcagctg gtactcgccc agcggatcgt cggacgaaac
     9541 gccccacaca ttgccgatat agctgtcgcc cggctcggac tggccgtagg tcgtggccgt
     9601 cctgccgtcc tccgacacag tcacgttggg attctcgtcg accccgtccc aggactgcgc
     9661 cggcgcgggc aggcgcagcg tctcgaccca tttcacgggc tggttcggat cccgggcgcg
     9721 cagccgccag ccgtaggcct gatctgccgt gcgcggaacc tccagcgtcg cgatgaagcg
     9781 cggatcgccg tcgggaccgg gctcccagat gccaaattcg atcccgacct cggaccgccg
     9841 ttcctgcgcc gcgccatcgg gcgtgcggtc ggaatgacag ccggtggcca gcacacacac
     9901 ggcgatcacg ggcatgaccc ggaggaagtg acgcaacacg attagtgaag ggttacgcat
     9961 ctggcggccg gcacgagcga aaggccggag tccaccctaa cggatagaca ccgcaggaat
    10021 ccagcggttc cgcgcgacta gttcttcgcc gggcgccagc ccgcagcgcg agcctcggct
    10081 tcagtgcaaa accagcgctc gcccttcgac acgtcgatct tcgtggcgtc gtacgaagcg
    10141 ctgcccggca catggtagat cctgccgttg gagccgatgt tgcccttgat caggcagtga
    10201 tcattcggtg ggagcggacc cggcgcaggc acagtctgct ggcctggtcc gcgcttcatg
    10261 tcggacgaac cagcgctttc gacgctgtgc aggtcgcggc tgaacccggc cgacgcctgc
    10321 atcgcgagga tgcagtttgc ggtcgtctcg tgcgaatagt cggtgcgcga ggcggcacgc
    10381 cctttctcga tggcccgcca ctcccacggg gcgacccacg tgcccggcgg caggctccac
    10441 aatcccaggc gcgatgcccg cgcgataccc tcgtaggcgc agaaactcgc gtcatccatg
    10501 tagtcccggt aggcccacgc gtcgccttgc tgtacgagcc aggcgttcac gttctcgctg
    10561 ccgagcagca cttcggccac cagacggtcg taccgatcct gggtcacgac gtcgagcgcg
    10621 acgatccggc cgtccacccg gctcgcgagg gctgctttcg cctgcggtcc ccagggttgg
    10681 tcatgctcgg gtgcatcgat cgaatgcagg cgcacgcgga tcggaccgga ctggagccgc
    10741 acgtcgatgg tgtcgccgtc gatcacccgc acgactgtgc cgaccaggac gggctggacg
    10801 gaatcaggaa ccgggcggcc atccgattgc ggcggcgcgg gtgcgggaac gtatggcacg
    10861 ccggccggtg ccggggctgc cggaggtcgc gcctgcggcg acgctgacgg ccgtactgaa
    10921 gtcgactcgg cgcaggaagc gagcgccaga caggcggcga gcaggttcgc gacgaccagg
    10981 ctggaagcgc accccggcgg ccctcgggtc acgccgcgct cgcaggtcgg gccagtatgt
    11041 gtcgcgcgag catcatggcc gcctcgaaca ggttcagcac cagtgcggtg acgtagaagg
    11101 cgggtgcgag tgacggcgcc actcccagga tcgcgagcac cagcgacacc tggatgggta
    11161 tttccaggaa cacccagaac aacaccgccc gcggcaggtt ggccttcgca tcctccaagt
    11221 gccatcggcg gaagtcgccg tcgctgaaag cgatcaggat ggttgcccag ataagcgcga
    11281 gaaatccggc gctcgaccac tcccagagtg ccgcgccacc cagcccgaac cgggcgagcc
    11341 acacgggaag gatcgccgac accagcacgg tgatgccgat gaaggcaacg gacaccaact
    11401 tgttccgggc gtccttcgac tgctcaccgg cggcgctgat caaggccccg aaaccaatga
    11461 agacgccggc aatctcggcg at
//
LOCUS       DAOMFD010000056        11293 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1537_length_11293_cov_36.486830, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000056 DAOMFD010000000
VERSION     DAOMFD010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11293)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11293)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11293
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1537_length_11293_cov_36.486830"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..363)
                     /locus_tag="PL152_04995"
     CDS             complement(<1..363)
                     /locus_tag="PL152_04995"
                     /inference="COORDINATES: protein motif:HMM:NF024581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQR48670.1"
                     /translation="MYARPAGALENTPRGHEAQRLLARCVHCGFCLPACPTYRVTGNE
                     LDSPRGRIYLVKQMVEGATAGAATLEHLDRCLTCRACETACPSGVEYGRIAELGRELA
                     EERVGRSPSQRVLRSAMAQ"
     gene            complement(366..1424)
                     /gene="glcE"
                     /locus_tag="PL152_05000"
     CDS             complement(366..1424)
                     /gene="glcE"
                     /locus_tag="PL152_05000"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: protein motif:HMM:NF008439.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HQR48671.1"
                     /translation="MADRDATAELVDRVRDATARRAPLRVVGGDTKRFLGRILECDLL
                     ELDVHTGIVSYDPAELVITARGGTRLDAIVAALGQHGQRLPFEPPSFGGAATLGGTVA
                     CGLSGPARAFAGPLRDYVLGARILAGDGRVLRFGGEVMKNVAGYDVARTMAGAFGTLG
                     VLLDVSLKVLPAARHTRTLALALDQQAALAHLEQLARTSLPLSASSWTDGMLWLRFEG
                     SERTLDDVQRRVGGEAVDDGAALWTSVREQTHRFFATGTRTWRLHVPASAPPLDLPAD
                     PLIEWNGMQRWYALDSTDVRDVAIAGGGHATLFRNARPEDTVFERPPEAVMRLHKALK
                     AVFDPAGILNPSRMYPDL"
     gene            complement(1428..2885)
                     /locus_tag="PL152_05005"
     CDS             complement(1428..2885)
                     /locus_tag="PL152_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HQR48672.1"
                     /translation="MAPDPALVRKLRESLGKEAVLASAESCVAFDCDALTAHRRTPDL
                     VVLPSDTAGVQAVLRIAAAHGTPVVARGAGTGLSGGALPVEGGILLVLSRLNRILEVD
                     PRRRLARVQPGVTNLAVSDAAAPHGLFYAPDPSSQVACTIGGNVAENSGGVHCLKYGL
                     TVHNLLEVVIATWDGELVTVGSPLGEAPGLSLLPLVTGSEGMLGVVVETTVRLVPVPE
                     RVELVLAAFDDVAACADAVGGIIAAGILPAGLEMMDGPAIRAVEAFVQCGYPVDAAAV
                     LLCELDGLAHDVEAQLAETVELLRRGGATSLRVARDAAERARMWSGRKSAFPAVGRLA
                     PDYYCMDGTIPRRQLAAVLARIAEMSREAGLAVANVFHAGDGNLHPLILFDAGQPGDL
                     ERAERLGAAILELCVAVGGTVTGEHGVGVEKLGPMCSQFGTPELEAFHSIKRAFDPHG
                     RLNPGKAVPTLARCAEYGAMRVHGGKLPHAHLPRF"
     gene            complement(2875..3540)
                     /gene="rpiA"
                     /locus_tag="PL152_05010"
     CDS             complement(2875..3540)
                     /gene="rpiA"
                     /locus_tag="PL152_05010"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HQR48673.1"
                     /translation="MTQDEKKRRAAESALQYVRDGEVIGVGTGSTVNFFIDALAAHKD
                     RIAGAVSSSEASTLRLRSHGIAVLDLNATGDLGVYVDGADEATRALELIKGGGGALTR
                     EKIVAGCSRQFVCIVDEQKVVERLGRYPLPVEVIPMARSHVARGLARLGGEPRLREGF
                     TTDNGNQILDVHGLDIPDPAALEAEINQITGVVTVGLFARRPADILLVGTTAGVVTLA
                     RGA"
     gene            complement(3846..4310)
                     /locus_tag="PL152_05015"
     CDS             complement(3846..4310)
                     /locus_tag="PL152_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HQR48674.1"
                     /translation="MNYWLFKSEPSTFGIDDLARAPDSTTAWDGVRNYQVRNLLRDTM
                     RKGDRGFFYHSSCAEPGIVGVVRVVRTGYPEAAQFDRKHRYHDPKSNPAEPTWYSVDV
                     RLERRIEPAITLETLRKHAHGALSGMLLLRRGNRLSITPVTRDEWRFILSLA"
     gene            complement(4307..4951)
                     /locus_tag="PL152_05020"
     CDS             complement(4307..4951)
                     /locus_tag="PL152_05020"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF013934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HQR48675.1"
                     /translation="MAQPTAETRAERRRLRALRSSLPRRQRVAAERAIVASLQRLQLL
                     RRGRYVAAYLAMPGEASLARAIDAARMTGATIHVPRITSRRRGEMRFVELRPDAALRA
                     NPRAFGIHEPSGGSAHIPLRRLDAVLLPVVGFDRAGHRLGMGAGYYDRALRRLRNASG
                     GWRRPRLVGIAYACQEVARIEAAPWDVALDAIVTEREVIVPRRSAAPANGSREP"
     gene            complement(4985..5170)
                     /gene="ssrS"
                     /locus_tag="PL152_05025"
     ncRNA           complement(4985..5170)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="PL152_05025"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(5203..5520)
                     /locus_tag="PL152_05030"
     CDS             complement(5203..5520)
                     /locus_tag="PL152_05030"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HQR48676.1"
                     /translation="MSEDKVSRVSVRLLDREYQVACPAEERGNLLDSAEYLDSKMREV
                     RDSGKVTGLDRIAVISALNLANELIKLRRQGTTVEGDVGARLRTLRERVEAALERGQQ
                     LEL"
     gene            complement(5517..5750)
                     /locus_tag="PL152_05035"
     CDS             complement(5517..5750)
                     /locus_tag="PL152_05035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02449 family protein"
                     /protein_id="HQR48677.1"
                     /translation="MTETPARSAVDAELRRLERRVDELVATIGQLKEENRALRQRQET
                     LMTERANLLQKNEQVRARVEAMIGRLKGMEHGA"
     gene            5830..6381
                     /locus_tag="PL152_05040"
     CDS             5830..6381
                     /locus_tag="PL152_05040"
                     /inference="COORDINATES: protein motif:HMM:NF015642.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein"
                     /protein_id="HQR48678.1"
                     /translation="MSGHAAFADVAQALDAAGSSVSAAEAHGCLCGAVCVRGDYGLAD
                     WLDEILPDAPAGGDSAFESLRSSTVAALAGDAMDFEPLLPVDDEPLPARVEALGAWCQ
                     GFLYGFGAAAPGSRHPLPEDVSEVLADLAQISNAGAVGSESAEAEEEAYAELVEFVRA
                     TVQLVYDELTPQRASQPMSRTHQ"
     gene            6389..7699
                     /gene="pepP"
                     /locus_tag="PL152_05045"
     CDS             6389..7699
                     /gene="pepP"
                     /locus_tag="PL152_05045"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HQR48679.1"
                     /translation="MTPRKDEFARRRKQLMRMMGKGGIAILPAAAEKQRNSDVHYNYR
                     PDSDFHYLTGFDEPEAVAVLVPGREQAEYILFVRDRDPLRETWDGRRAGPEGATRDYG
                     ADDAFPIGDIDDILPGLMEQCSRVYYTMGVHAEFDHHVVSWVNTLRSQAKHGLHTPQE
                     FVALDHLLHDMRLYKSRNELALMRHAAQITVAAHIRAMKSTRPGMHEYQVMAEVLHEF
                     HSNGGDIAYHPIVGGGANGCVLHYRENADPLHDGDLLLIDAGCEYRYYAADVTRTFPV
                     NGRFTPEQRAVYEVVLEAQEAAFAAIRPGNHWNEPHDAAVRAITQGLVRIGLLEGRVA
                     SLVKEEAYKKFYMHRTGHWLGMDVHDVGDYKVGSEWRVLEPGMVMTVEPGIYIPAGTK
                     GVPKRWWNIGVRIEDDVAVTADGCEILTAGLPRTPSEIERLMAA"
     gene            7715..8971
                     /gene="ubiH"
                     /locus_tag="PL152_05050"
                     /gene_synonym="visB"
     CDS             7715..8971
                     /gene="ubiH"
                     /locus_tag="PL152_05050"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF004356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008681 - 2-octaprenyl-6-methoxyphenol
                     hydroxylase activity [Evidence IEA];
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HQR48680.1"
                     /translation="MTGATSDSFDYDLAIAGGGLVGASLALALAPLGLRTVVVEAVAP
                     GVGGHPSYDERTTALANGTVRAFQGLGVWRQMEREATPIRRIHVSEQGRFGIARIDAA
                     EQGLESLGYVLPNRVMGAALWAGLEAVRGVEVIAPARVVSTSAGADARTLEIEQDGRL
                     IRLRARLVVAADGARSVIREQAGIPAEQWDYGQTAIVTTVTTQRFHDHVAYERFTPDG
                     PIAVLPLADGRCGVVWTRKLEEAARLLALPDDEFLAELQVAFGFRLGRLLKVGARHSY
                     DLSLTRSDRHVGPRLAIVGAAAQGLHPIAGQGFNIGMRDAACLAEVIADARAEGRTDP
                     GDGQLLRAYADWREEDRRRIVAFTDGMVRLFGTPLGVLRGLRSVGLLAFDAFPPAKAA
                     MARLSVGAAGRVPRLVRGVPLTGALS"
     gene            8968..10215
                     /locus_tag="PL152_05055"
     CDS             8968..10215
                     /locus_tag="PL152_05055"
                     /inference="COORDINATES: protein motif:HMM:TIGR01988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HQR48681.1"
                     /translation="MSLGRRLDFDVVVIGGGMVGLAVAALLSREERLSGWRIAVVEPA
                     APHRPTDGLVDLRVSALSRASQRILGAAGAWPAIEPYACPYERMVVWDAASTCDARDA
                     LRFDAAETGEPDLGHIAENIRVQWALAEAPALRGVTQLRASLESLDFDVESAKVSLSD
                     GRRIAAGLVIGADGGPSKVRELCGIGRAGWAYGQTAVVAHLRTERPHARTAWQRFLPE
                     GPLAFLPLRDGRVSLVWTTTPAAAAALQAAEPADFSRQVTDASDRVLGAAALDSGLAA
                     FPLALWHSREYVRPRLALVGDAAHTIHPLAGQGVNLGFLDAASLVQVLGEAVDAGADP
                     AGLRTLRRYARWRRAENALLLGTTDTLNRLFTERSVGIAAARRLGLALVGSQPLARRL
                     LVRRALGLTGDLPAVVVDRSRAS"
     gene            10212..>11293
                     /locus_tag="PL152_05060"
     CDS             10212..>11293
                     /locus_tag="PL152_05060"
                     /inference="COORDINATES: protein motif:HMM:NF036723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48682.1"
                     /translation="MTDGDWQHDLFTQAPPELGNQYRSDPLLGSWLKRSLPADVRSAL
                     APELDEIGELAGGRLYSMQLADRVNEPRLVQWDAWGNRVDAVEVTPLWREAEQLATRH
                     GLVATAYEPAHGRYARIAQFAKVFLFHPSSDVYSCPLAMTDGAARSLLESGDQRLIER
                     AVPRLTSRDPGTAWTSGQWMTETTGGSDVGRSQTRAVRDGDGWRLYGRKWFTSSVAAQ
                     MALTLARPEGNEPGGKGLAMFYVEVRDATGRLNDLRVDRLKDKLGTRKVPTAELMLEG
                     TRASLVGQPFNGTRAIEPMLAVTRMWNSVCAVAAMRRGLALARSYASRREVFGRPLAD
                     QPLHSDTLAALEAETWGAFLMTFLLV"
BASE COUNT         1634 a         3900 c         4101 g         1658 t
ORIGIN      
        1 ctgtgccatg gcggatcgca ggacgcgctg ggacggcgag cggccgacgc gctcctcggc
       61 gagttcgcgg ccgagctccg cgatgcggcc gtactcgaca ccggacgggc acgcggtctc
      121 gcaggcacga caggtgaggc agcgatcgag gtgctcgagc gtagctgcgc cggcggtcgc
      181 gccttcgacc atctgcttga cgaggtagat gcggccgcgc gggctgtcga gttcgttgcc
      241 cgtgacccga taggtggggc aggccggtag gcagaacccg cagtgcacac aacgcgcgag
      301 cagtcgctgg gcttcgtggc cgcgtggtgt gttctcgagg gcgccggcgg gacgcgcgta
      361 catggttaca gatcggggta catgcggctg ggattcagga tgccggccgg gtcgaacacc
      421 gccttcagcg ctttgtgcaa gcgcatcacg gcctcgggcg gccgctcgaa gaccgtgtcc
      481 tccggtcgcg cgttgcggaa cagggtcgcg tggccgccgc cggcgatcgc cacgtcgcgc
      541 acgtcggtcg agtcgagcgc gtaccagcgc tgcatcccgt tccactcgat gagcgggtcg
      601 gcgggcaagt ccaggggcgg ggccgatgcg ggcacgtgca gccgccaggt ccgcgtaccg
      661 gtcgcgaaga atcgatgcgt ctgctcgcgg acggacgtcc agagcgcggc gccgtcatcg
      721 accgcctcgc cgccgaccct gcgctgcacg tcgtcgagcg tgcgctcgct gccttcgaag
      781 cgcagccaca gcattccgtc ggtccagctg ctggccgaga gcggcaggga cgtgcgtgcc
      841 agctgctcga ggtgcgccag cgcggcctgc tggtcgagcg caagcgcgag cgtgcgggtg
      901 tgtcgggccg cgggcagcac cttgagcgac acgtcgagca gcacgcccag ggtgccgaac
      961 gcaccggcca tggtgcgcgc gacgtcgtag ccggccacgt tcttcatcac ctcgccgccg
     1021 aagcgcagca cccgaccgtc gccggcgagg attcttgcgc cgagcacgta atcgcgcaga
     1081 ggcccggcga atgcccgcgc gggaccgctg agtccgcagg cgaccgtgcc gccgagcgtc
     1141 gccgcgccgc cgaacgacgg cggctcgaac ggcaaccgct gcccgtgctg ccccagcgcg
     1201 gccacgatcg cgtcgaggcg cgtgccgccc cgggccgtga tgaccagctc ggcagggtcg
     1261 taggagacga tgcccgtgtg cacgtcgagt tcgagcagat cgcactcgag gatgcggccc
     1321 aggaatcgct tggtgtcgcc accgacgacg cgcagcggcg cgcggcgtgc ggtcgcgtcc
     1381 cgcacccggt cgaccagctc ggcggtggca tcgcggtcag ccatgactca gaaccgcggg
     1441 agatgcgcat gcggcagctt gccaccgtgc acgcgcatcg ccccgtactc cgcgcagcgc
     1501 gcgagcgtcg gcacggcctt gcccgggttc aaccggccgt gaggatcgaa ggcgcgcttg
     1561 atcgagtgga aggcctcgag ctccggcgtg ccgaactggc tgcacatcgg accgagcttc
     1621 tcgacgccga cgccgtgttc cccggtgacc gtgccgccga ccgcgacgca gagctcgagg
     1681 atcgcggcgc cgagccgttc cgccctttcg agatcgccgg gctgtccggc atcgaacagg
     1741 atcaacgggt gcaggttgcc gtcgccggcg tggaaaacgt tggccacggc cagccccgcc
     1801 tcccgcgaca tctccgcgat gcgggcgagc acggcggcga gctggcggcg cgggatggtg
     1861 ccgtccatgc agtagtaatc cggtgccagc cgcccgacgg cgggaaaggc ggacttgcga
     1921 cccgaccaca tgcgggcgcg ctcggcggcg tccctcgcga cgcgcaggct cgtcgccccg
     1981 ccgcggcgca gcagttcgac cgtctctgcg agctgcgctt cgacgtcgtg tgccaggccg
     2041 tcgagttcgc agagcagcac ggccgccgcg tctaccgggt agccgcattg cacgaaggcc
     2101 tcgacggcgc gtatcgccgg cccgtccatc atctcgagtc ccgccggcag gatgccggcg
     2161 gcgatgatgc cgcccaccgc gtcggcgcag gcagcgacgt cgtcgaatgc cgccagcacc
     2221 agctcgacgc gttccgggac cggcacgagt cgcaccgtcg tctcgaccac gaccccgagc
     2281 atgccctcgg acccggtgac cagcggcagc agcgacaggc ccggtgcctc gccgagtggc
     2341 gatccgacgg tgacgagttc gccgtcccag gtggcgatca ccacctcgag caggttgtgc
     2401 accgtgagtc cgtacttgag gcaatgcacg ccgccggaat tctcggcgac gttgccgccg
     2461 atcgtgcagg cgacctggga cgaggggtcc ggcgcgtaga agagcccgtg cggcgccgcc
     2521 gcgtccgaga ccgcgaggtt cgtgacgccg ggctgcacgc gcgcgagccg ccggcgcggg
     2581 tccacctcga ggatgcggtt cagcctggag agcacgagca ggatcccgcc ctcgaccggc
     2641 aacgcgccgc ccgagaggcc cgtgcctgcg ccgcgagcga cgaccggcgt gccatgggcc
     2701 gcggcgatgc gcaacaccgc ctgcaccccg gccgtgtcgg atggcaggac gacgaggtcc
     2761 ggcgtgcggc ggtgcgccgt cagtgcgtcg cagtcgaagg cgacgcagga ctccgccgaa
     2821 gcaaggacag cctccttgcc gagggactcg cggagcttcc ggacgagtgc gggatcaggc
     2881 gccacgcgcc aacgttacga cgccggcggt ggttccgacc agcagtatgt ccgccggccg
     2941 tcgtgcgaag aggccgacgg tgaccacgcc ggtgatctgg ttgatctcgg cctcgagcgc
     3001 cgccgggtcc gggatgtcga gcccgtggac gtcgagaatc tggttgccgt tgtccgtcgt
     3061 gaacccctcg cgcaggcgtg gctccccgcc gagacgggcg agtccgcgcg cgacgtggga
     3121 gcgtgccatc gggatcacct cgaccggcag cgggtagcgg ccgagacgct cgaccacctt
     3181 ctgctcgtcc acgatgcaga cgaactgccg ggagcagccc gcgacgatct tctcccgggt
     3241 gagggcgccg ccgccgccct tgatgagctc gagcgcgcgg gttgcctcgt cggcgccgtc
     3301 cacgtacacg ccgaggtcgc cggtggcatt caggtccagg acggcgatgc cgtgggagcg
     3361 cagtcgcagc gtgctcgcct ccgagctcga caccgcgccc gcgatgcggt ccttgtgtgc
     3421 cgccagcgcg tcgatgaaga aattgaccgt ggagccggtg ccgactccga tcacctcgcc
     3481 gtcgcgcacg tactggaggg ccgactcggc cgctcgccgc ttcttctcgt cctgcgtcat
     3541 ggttttcgtc ctccgaaaac aactgtgccc gctttcgcgg gcacagtcgt gaagcttcgt
     3601 tcggaagctt acttcttctt ggccggggcc ttcttagcct tagccttctt cgctgccttc
     3661 ttcttcgctg ccattgtcag agtctccatg tcgggttagt ccgggcgcag tatttacaga
     3721 aatcaaatat caaatgcaag aggcacgtga cgaattccga ttgacccctc gacgcgatcg
     3781 tgctcgcccg atgactcgac gagcgagcgc atcgtcgcgc ccgcgctgtg attcacggca
     3841 ttcgctcacg cgagcgagag gatgaaacgc cactcgtcgc gcgtcaccgg cgtgatcgag
     3901 aggcggttgc cgcgacggag cagcagcatg ccggagagcg ctccgtgcgc gtgtttgcgc
     3961 aaggtttcga gcgtgatcgc gggctcgatg cgacgctcga gccgcacgtc gacgctgtac
     4021 cacgtgggct cggccggatt gctcttcgga tcgtggtagc gatgcttccg atcgaattgc
     4081 gctgcttccg gatagcccgt gcgcaccacg cggacgacgc cgacgatgcc gggctcggcg
     4141 cagctcgagt gatagaagaa gccgcggtcg cccttgcgca tcgtgtcgcg cagcaggttg
     4201 cggacctggt agttgcgcac gccgtcccag gcggtcgtcg aatcgggcgc acgggcgagg
     4261 tcgtcgatgc cgaaggtcga cggctcggac ttgaacagcc agtaattcac ggctcgcggc
     4321 tcccgttggc cggcgccgcc gagcggcgcg ggacgatgac ttcgcgctcg gtgacgatcg
     4381 cgtcgagcgc gacgtcccag ggggcggcct cgatgcgcgc aacctcctgg caggcgtagg
     4441 cgatgccgac gagtcgcggc cgtcgccagc cgccgctcgc attgcgcagt cggcgcagcg
     4501 cccggtcgta gtagccggca cccatgccga ggcgatgtcc ggcgcggtcg aagccgacca
     4561 cgggcagcag cacggcatcg agccggcgca ggggtatgtg agcggacccg ccgctcggtt
     4621 cgtggatgcc gaacgcgcgc ggattggccc ggagcgccgc gtcgggccgc agttcgacga
     4681 agcgcatctc gccgcggcgc cgactcgtga tccgcggcac gtggatggtc gccccggtca
     4741 tgcgcgcggc gtcgatcgcg cgagcgaggc tcgcctcgcc gggcattgcg aggtacgcgg
     4801 cgacgtatcg gccgcggcgc aggagttgca ggcgttgcag cgacgccacg atcgcgcgct
     4861 ccgcggcgac gcgctggcgg cgcgggagcg aggaccgcag tgcgcgcagc ctgcggcgct
     4921 cggcgcgggt ctcggcggtg ggctgagcca tgcgtccgtg tcgtccgctg gaagaaaggg
     4981 gatggcgcca cccgcctgtg ccgtcacgga ccattgctct cgacaagagc aacaggcggg
     5041 gaaggccacg gcacctcagg cttcccgctc ggggcggact tgcacaacag tgccgcacgg
     5101 ccggctcccc tgggtttttt gacttacggt cgagaggtaa cccggaccgt gtcgaacacc
     5161 gcaggcggcg ccaaatcgtc gggcgacggt cttgccgtcg cctcacagtt ccagttgctg
     5221 ccctcgctcc agggccgcct cgactcgctc gcgcagggtg cgcagccgcg cgccgacgtc
     5281 gccttcgacc gtcgtgccct gccgtcgcag cttgatcagc tcgttggcga ggttgagcgc
     5341 cgagatcacg gcgatgcggt cgaggccggt gaccttgccg gaatcgcgca cctcgcgcat
     5401 cttcgagtcg aggtactcgg cggagtcgag caggttgccg cgctcctcgg cggggcaggc
     5461 gacctggtac tcgcggtcga gcagccgcac gctgacgcgt gacaccttgt cctcgctcat
     5521 gcgccgtgct ccatgccctt caggcggccg atcatcgcct cgacgcgcgc ccgcacctgc
     5581 tcgttcttct gcagcaggtt ggcgcgctcg gtcatgaggg tttcctggcg ctggcgcagc
     5641 gcccggttct cttccttcag ctgcccgatc gtggcgacga gctcgtccac gcgacgctcc
     5701 aggcgacgga gctcggcgtc gacggcggat cttgctgggg tttccgtcat gcgaagaact
     5761 atagggccgc accacggggg aatcaattga accggcggcc gggcggcgcg cgatgttacg
     5821 ataatcgcga tgagcggaca cgcagcattc gccgacgtcg cgcaggcgct cgacgccgcc
     5881 ggatcgtcgg tcagcgcggc ggaggcccac gggtgcctgt gcggggcggt gtgcgtccgc
     5941 ggcgactacg ggttggccga ctggctcgac gagatcctgc ccgacgcacc tgcaggcggc
     6001 gacagtgcgt tcgaatctct gcgatcgagc accgtcgcgg cgctggcggg cgacgcgatg
     6061 gatttcgagc cgctgctgcc ggtcgacgac gagccgctgc cggcccgcgt cgaggcgctc
     6121 ggcgcctggt gccagggatt cctctacgga ttcggcgcgg cggcgcccgg cagtcgccac
     6181 ccgctgcccg aggacgtgtc cgaggtcctg gccgacctgg cgcagatctc caatgccggc
     6241 gcggtcgggt cggagagcgc ggaggcggag gaggaagcct atgccgagct cgtcgagttc
     6301 gtccgggcca cggtgcaact cgtctacgac gagctcacgc cgcagcgcgc gagccagccg
     6361 atgtcccgca cccaccaatg agtgaccgat gacgcccaga aaggacgagt tcgcgcgacg
     6421 ccgcaagcag ctgatgcgga tgatgggcaa gggcggcatc gccatcctgc ccgcggccgc
     6481 cgagaagcag cgcaacagcg acgtgcacta caactatcgg ccggacagcg acttccatta
     6541 cctcaccgga ttcgacgagc ccgaggccgt ggccgtcctc gtgccgggaa gggagcaggc
     6601 cgagtacatc ctgttcgtgc gcgaccgcga cccgctgcgg gagacctggg acggcaggcg
     6661 ggccggcccg gagggggcga cccgggacta cggggcggac gatgcgtttc cgatcggcga
     6721 catcgacgac atcctgcccg gccttatgga gcagtgcagc cgcgtctact acacgatggg
     6781 cgtgcacgcc gaattcgacc atcacgtcgt ctcctgggtg aacaccctgc gcagccaggc
     6841 caagcacggc ctgcacacgc cgcaggaatt cgtggcgctc gaccacctgc tgcacgacat
     6901 gcgcctctac aagagccgca acgagctcgc gctcatgcgc cacgcggcgc agatcacggt
     6961 cgcggcgcac atccgggcga tgaagagcac gcgccccggc atgcacgaat accaggtgat
     7021 ggccgaggtc ctgcacgagt tccacagcaa cggcggggac atcgcgtatc acccgatcgt
     7081 cggcggtggc gccaacggct gcgtcctgca ctaccgggaa aatgccgacc cgctgcacga
     7141 cggcgacctg ctgctcatcg acgcgggctg cgagtaccgt tactacgcgg cggacgtgac
     7201 gcggaccttc ccggtcaatg gacgattcac gccggagcag cgcgccgtgt acgaggtggt
     7261 gctggaggcg caggaggccg cgttcgccgc gatccggccc ggcaaccact ggaacgagcc
     7321 gcacgacgcg gcggtgcggg ccatcaccca gggtctcgtg cgcatcgggc tgctcgaggg
     7381 ccgcgtcgcc tcgctggtca aggaagaggc ctacaagaag ttctacatgc accgcaccgg
     7441 ccactggctg ggcatggacg tgcacgacgt cggcgactac aaggtcggca gcgagtggcg
     7501 ggtgctcgag cccggcatgg tgatgaccgt ggaaccgggc atctacatcc cggccggcac
     7561 caagggcgtg ccgaagcgct ggtggaacat cggcgtgcgc atcgaagacg acgtggccgt
     7621 gacggcggac ggctgcgaga tcctgacggc gggcctgccg cgcacaccat ccgagatcga
     7681 gcggctgatg gcggcctgac gggctgcacg tgagatgacc ggcgcgacca gcgactcctt
     7741 tgactacgac ctcgccatcg caggcggtgg cctcgtcggg gcatccctcg cgctggcgct
     7801 cgcgccgctc gggctcagga ccgtggtcgt cgaagcggtg gcgcccggcg tcggcgggca
     7861 tccgagctac gacgagcgca ccaccgcgct cgcgaacggc accgtgcggg ccttccaggg
     7921 tctcggcgtc tggcgacaga tggagcgcga ggccactccg atccgccgca tccacgtgtc
     7981 ggagcagggg cgattcggca tcgcccgcat cgacgccgcc gagcaggggc tcgagtcgct
     8041 gggctacgtc cttccgaacc gcgtcatggg cgccgccctc tgggcaggcc tcgaggcggt
     8101 gcgcggcgtc gaggtcatcg cgcccgcccg cgtggtctcg acgagcgccg gcgccgacgc
     8161 gcgcacgctg gagatcgagc aggacgggcg tttgatccgg cttcgcgcgc gcctcgtcgt
     8221 cgccgcggac ggcgcccgct ccgtgatccg cgagcaggcc ggcataccgg ccgagcaatg
     8281 ggactacggg cagaccgcga tcgtcaccac ggtgacgaca cagaggttcc acgatcacgt
     8341 cgcctacgag cgcttcacgc cggacggccc gatcgcggtg ctgccgctcg cggacggtcg
     8401 ttgcggcgtg gtgtggacgc gcaagctcga ggaggcggcc cgattgctcg cgctgcccga
     8461 cgacgagttc ctggcggagc tgcaggtggc gttcggtttc cggctgggcc ggctgctcaa
     8521 ggtcggcgcg cgccactcct atgatctctc gctgacgcgc tcggaccgtc acgtgggacc
     8581 gcgcctcgcg atcgtcggcg cggcggcgca ggggctgcac ccgatcgcgg ggcagggctt
     8641 caacatcggg atgcgcgatg cggcctgcct cgccgaggtg atcgccgacg cgcgcgccga
     8701 agggcgcacc gacccgggtg acgggcagct gctgcgcgcc tatgccgact ggcgcgagga
     8761 ggaccgccga cggatcgtgg ccttcacgga cggcatggta cggctcttcg gaacgccgct
     8821 cggcgtgttg agggggttgc gcagcgtggg cctgctcgcg ttcgacgcat tcccgcccgc
     8881 caaggctgcg atggcgcgcc tgagcgtggg cgccgcgggg cgagtgccca ggctcgtgcg
     8941 cggcgtgccg ctcaccgggg ccttgtcgtg agcctcggtc gccggctcga tttcgacgtc
     9001 gtcgtcatcg gcggcggcat ggtgggactg gcggttgccg cgctgctgtc ccgggaggag
     9061 cggttgtccg gatggcgcat cgccgtcgtg gagccagccg caccgcaccg gccgacggat
     9121 ggcctggtcg acctgcgcgt gtccgcgctg tcgagagcat cgcaacgcat cctcggagcc
     9181 gcgggcgcct ggcccgccat cgaaccgtac gcctgtccgt acgagcgcat ggtggtctgg
     9241 gacgcggcaa gcacttgtga cgcgcgggat gccctacgct tcgatgcagc ggaaaccggt
     9301 gagcccgacc tggggcacat cgcggagaac atccgcgtgc agtgggcgct cgccgaagcg
     9361 cccgctctgc gcggggtgac ccagctcagg gccagcctcg aatcgctcga cttcgacgtg
     9421 gagtccgcga aggtctcgct gagcgacgga cgtcggatcg ccgccgggct ggtgatcgga
     9481 gcggacggcg ggccgtcgaa agtccgcgaa ctgtgcggca tcgggcgggc cggctgggcg
     9541 tacggccaga cggccgtggt cgcgcacctg cgcacggagc gcccgcatgc acgcaccgcg
     9601 tggcagcgat tcctgccgga gggtccgctt gcgttcctgc cattgcgcga cggtcgcgtg
     9661 tcgctggtgt ggacgacgac gcccgcggcg gcggcggcgt tgcaggcggc cgagccggcg
     9721 gatttcagcc gacaggtgac cgacgcgagc gaccgagtgc tcggtgccgc ggccttggac
     9781 agcggactcg ccgcatttcc gctcgccttg tggcactcac gcgagtatgt ccgaccccgg
     9841 ctggcgctcg tcggggatgc ggcccacacg attcacccgc tcgcggggca gggcgtcaac
     9901 ctcggattcc tcgatgcggc gtcgctcgtg caggtgctcg gcgaagccgt cgacgccggc
     9961 gcggacccgg cgggcctgcg cacgttgcgc cgctacgcac ggtggcgacg tgcggagaat
    10021 gcattgctgc tcggcacgac cgatacgctg aatcgcctct tcaccgagcg cagcgtcggg
    10081 atcgcggccg cgcggcggct cggactcgca ctcgtcggct cgcagccgct ggcgcgccgg
    10141 ctgctcgtgc gccgggcgct ggggctcacg ggcgacctgc cggcggtcgt tgttgaccgg
    10201 agccgcgcgt cgtgaccgac ggcgactggc agcacgacct gttcacgcag gcgccgccgg
    10261 aactcggcaa ccagtaccgc agcgacccgc tgctcggcag ctggctgaag cgcagcctcc
    10321 cggcggacgt gcgcagtgcg ctcgcgccgg agctcgacga gatcggcgaa ctcgccggtg
    10381 gcaggctgta ctcgatgcag ctcgcggacc gcgtcaacga gccgcggctg gtgcagtggg
    10441 atgcgtgggg caaccgtgtc gacgcggtgg aggtgacgcc tctgtggcgc gaggcggagc
    10501 agctcgcgac ccggcacggg ctggtcgcga cggcctacga gccggcgcac ggccgctacg
    10561 cgcgcatcgc gcagttcgcc aaggtgttcc tcttccatcc gtcgtcggac gtctacagct
    10621 gcccgctcgc gatgaccgac ggcgcagcgc ggtcgctcct cgagtcgggc gaccagcgtc
    10681 tcatcgaacg cgccgtgcca cgccttacgt cgcgcgaccc cgggactgcg tggaccagcg
    10741 ggcagtggat gaccgagacg accggcggct ccgacgtcgg ccgctcacag acccgggcgg
    10801 tccgcgacgg cgacggctgg cggctctacg gacgcaagtg gttcacctcg tcggtggcag
    10861 cgcagatggc gctcacgctc gcgcgtcccg agggcaacga accgggcggc aaggggcttg
    10921 cgatgttcta cgtcgaggtc cgcgacgcga cgggccggtt gaacgacctg cgcgtcgatc
    10981 gcctgaagga caagctcggc acgcgcaagg tgccgacggc cgagctgatg ctggaaggca
    11041 cgcgcgcctc gctcgtcggc cagccgttca acgggacgcg ggcgatcgag cccatgctcg
    11101 ccgtgacgcg catgtggaac agcgtgtgcg cggtggccgc catgcgccgc ggtctggcgc
    11161 tcgcacgctc ctacgcgtcg cgacgcgagg tgttcggccg gccgctcgcg gaccagcccc
    11221 tgcactcgga cacgctggct gccctcgagg ccgagacctg gggcgcgttc ctgatgacgt
    11281 tcctgctggt cga
//
LOCUS       DAOMFD010000057        11256 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1550_length_11256_cov_33.867869, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000057 DAOMFD010000000
VERSION     DAOMFD010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 11256)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11256)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11256
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1550_length_11256_cov_33.867869"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1067
                     /locus_tag="PL152_05065"
     CDS             <1..1067
                     /locus_tag="PL152_05065"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR48683.1"
                     /translation="ALGLANMAHMEPVGHLGTVAALALLVAFSSATQDIAVDAWRIEA
                     APPLMQGAMAASYQLGYRIALMVASAGALWIAADLGWDTAYTAMAALVGIGLVTTLVI
                     PEPDARIPRDSLDQEARVVAWLERKAHWPEPLRRVGAWFTGAVVCPFVDFFQRYGTRL
                     AILMLAFIATYRLADFTMGVMTNPFYLDMGFSLKEIAAVAKGFGVFMSILGTLVGGVA
                     VVRLGLVRTLVLGCSLIIAANLMFAVFAWTGKPSLAGLAVVISADNVAMGIAGTALIA
                     YLSSLTSASYTATQYALFSSMYALPGKLLMGASGFVVDAIGYPAFFVYTATLGVPALV
                     MLYIVTCTAKRAAPAAVGAA"
     gene            1067..1450
                     /locus_tag="PL152_05070"
     CDS             1067..1450
                     /locus_tag="PL152_05070"
                     /inference="COORDINATES: protein motif:HMM:NF012575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HQR48684.1"
                     /translation="MEETDLERVICPLHELADPGAREFTMGNGDWPLRGFVVRSGETV
                     RAYVNHCPHAGFPLNWTPDGFLAPHSRLILCSMHGAVFEIETGLCISGPCDGLRLRAL
                     PVRVERGYVMLGDEVPLEEPPDLGS"
     gene            complement(1473..1826)
                     /locus_tag="PL152_05075"
     CDS             complement(1473..1826)
                     /locus_tag="PL152_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48685.1"
                     /translation="MAKLAPVIGQWYRRPGGDSFEVVAIDRDDRTIEIQYFDGTVEEL
                     EVDEWLEDEFESTEPPEDWTGSLDVEPEDTENEYEAEPGTAKAWADPLQFVDRGEAAG
                     YSEVDLPEAEEGERY"
     gene            1918..2559
                     /locus_tag="PL152_05080"
     CDS             1918..2559
                     /locus_tag="PL152_05080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family protein"
                     /protein_id="HQR48686.1"
                     /translation="MRGLPMFDELVKFFVVLLVVVEPLSLLPVLAGLTDGADDGYRRR
                     MSVKAVGISVAVCLVFALGGSALLQVLGITVDAFKVGGGILLFLLAVEMVFARPSGVR
                     SITPGEDAEAHHRGDISVFPLAFPLIAGPGALATILLAFAGVEPGSLQFAAQVGIILV
                     VLGITLALMLLTGPVMRVIGVTGGAVLGRLLGVLLAALASQFVLDGIRGAIAS"
     gene            complement(2546..3169)
                     /gene="trmB"
                     /locus_tag="PL152_05085"
     CDS             complement(2546..3169)
                     /gene="trmB"
                     /locus_tag="PL152_05085"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HQR48687.1"
                     /translation="MTVAQERAWRELWPRYGIDDGDGGLDFAALFGRTAPVTLEIGFG
                     NGESLVSLAAEHPGEDFVGIEVHRPGVGHLLLLAGALGLSNLRVACRDAVEVLERRIA
                     DASLDTVLLYFPDPWPKKRHHKRRIVQPAFVDLVARKLRPGGRFRLATDWQHYAEQML
                     AVAGGCGSLANEFDGYCPRPDSRPLTRFESRGQRLGHEVWDLCFRKR"
     gene            3333..3938
                     /locus_tag="PL152_05090"
     CDS             3333..3938
                     /locus_tag="PL152_05090"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HQR48688.1"
                     /translation="MASTTGMLDNLGANAHALCRRTWWVFLVGGLASVAFGVLAFANP
                     GLALFVLAAFFAASILVDGAFNILGALQHREKDGWWVMLLMGLLGVLVGGYALWHPPI
                     SMLALVYIVAFQAIMLGVFLVTLGYKVRKATTREWILYAAGGLSILFGVLVIVNPLAG
                     AASIVYLIACWAVVVGALKVWFGFKVKSLPTKLGDRMSALH"
     gene            complement(3948..5546)
                     /locus_tag="PL152_05095"
     CDS             complement(3948..5546)
                     /locus_tag="PL152_05095"
                     /inference="COORDINATES: protein motif:HMM:NF009472.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HQR48689.1"
                     /translation="MLRYKADVVVIGSGIAGVAAAIELLGRGRRVLLIDRDAEENMGG
                     LAKESFGGIWFAGTPLQRRYGIRDGVEQGLRDWKAFAEFGPDDRWPLAWAESYVQRAN
                     EEIFDWLRGIGVEFMPMPLWVERGLYTPGNSVPRWHIVWGTGHELAVVLNRHLLAHPR
                     RPLLELRFGHRVESLVVTNGSVSGCRGVMEGSAQEFEASGESVVIASGGINGNLERVK
                     ANWHADWGKPPETILNGSHKYADGRLHDAASTLGARITHLDWQWNYAAGVHHWKPRKP
                     SHGLSLVPPKSALWLNWRGERIGPMPLVTGYDTHDLVAQICRQERAYSWQLLNRRIAL
                     KELAISGGEFNPSFREKKRLHVARDMLRGNRWLYDEVTTNCKDFVVADTLPELVEKMN
                     ALTGDRSVDLAAITEAATTYDARIDLGPRFHDDDQLRRIEFARRWIGDRLRTCKFQKI
                     LEPAAGPLLAIREFIISRKSMGGLQTDLSSRVLDGSGQPIPGLYAAGESAGFGGGGMN
                     GKRGLEGTFLGGCLFGGRIAGRVA"
     gene            complement(5550..6164)
                     /locus_tag="PL152_05100"
     CDS             complement(5550..6164)
                     /locus_tag="PL152_05100"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQR48690.1"
                     /translation="MDRIKEFLKTTVIGGLVFLVPLVVLALLLRKALEMARKVADPIA
                     ALFPAHQFAGVAVGTIVAALVLLVLAFVAGLLARTNTGRAMTKWIGDSLLGNLPQYRV
                     VTTMAEGLTKVEQGQGLAPVLVCLDDAWQLGYALPEPMPAGWTAVFIPQSPTPMSGNV
                     VYARASRVRPLNIGMPMAAKLVKQMGVGSAETLDGVDLTLAEGD"
     gene            complement(6204..6659)
                     /locus_tag="PL152_05105"
     CDS             complement(6204..6659)
                     /locus_tag="PL152_05105"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HQR48691.1"
                     /translation="MTRIAPHRLDPTRYPLKVRIIARYADVDPLWHINNVAIAQYYEE
                     ARVSALTHMLGGARIPTPAGERILIAHQSIDYLLEAGYPGTLEVGVGVLRLGNTSYTL
                     AMGMFQDGRCVSVSDAVMVFADSTGPARIPDSYRDRARPWLLGEVAADR"
     gene            6708..7382
                     /locus_tag="PL152_05110"
     CDS             6708..7382
                     /locus_tag="PL152_05110"
                     /inference="COORDINATES: protein motif:HMM:NF017833.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HQR48692.1"
                     /translation="MTAVQFRNTEVRLSEALAATAAAVRGHVVTLRELLGYVGEQGLL
                     VFSAILALPFLIPVSLPFMSTALGLPMLLIGIAVIAGRLPWLPARLLDHELPSETVQH
                     LLERATRMALRFEHLVKPRLPVLTGSGFVNSLHGVTLVTAVLLLMAPLPFVPLANTLP
                     GIAIILLCLGIAERDGLLLVVGYFVALVSAVYIGSLMWLVVQAGMNFDDVLAEPGAWF
                     SRIFKD"
     gene            7511..9574
                     /locus_tag="PL152_05115"
     CDS             7511..9574
                     /locus_tag="PL152_05115"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HQR48693.1"
                     /translation="MKIWSMLSTIAVALLLAAGVADAAGTRPFTPKDLVMLDRASEPR
                     LSPDGKVLAYTLRETDLDANRGARSIWRLDLADQNAHPQRVTAAGSNAWSPRWSPDGR
                     TLYFLSDRAGSDQVWALTSGLGDARQVTKLPLDVGSFVVAPDGRRLAVSLEVFPDCAD
                     LACTKQRRDDRAAAKVSGVVYDKLFIRHWDAWSDGSRSQLFIADVGADGVVSQEPRLI
                     TRGIDGDVPSKPFGDDSEYAFSPDGKTVVFNARIAGTTEAWSTNFDIYSVATDGDAAP
                     RNLTSGNAAWDGYPLPSPDGRKVYYLAMRKPMHEADRFGIMELDLATGRSREIAPDWD
                     RSAGPLKISADGRTLYTSADDDGRHPLFAVDVASGRVRTVAQGGDVTDFSLAAGTIVV
                     ARASLKAPTDFYRVAPGAERRLTDLNAARLRDVVMGDVEVFEFKGWNDERVRGYVVKP
                     YGFEPGKKYPVAFLIHGGPEGSWLDEFHYRWNPQTYAGAGFAVVAIDFHGSTGYGQAF
                     TEAILEHAGDRPFEDLQKGWAAALAKFDFLDAGRACALGASYGGYMVNWIAGNWQAPW
                     KCLVSHDGIFDSRSMYYTTEEIWFDETEHGAPQYERPQAYEQFNPINHVAEWKVPMLV
                     VHGAKDYRIPLEQGIGAFTALQRRGIPSQFLTFPDENHWVLKPQNSLKWHETVEAWIK
                     RWTAP"
     gene            9712..>11256
                     /locus_tag="PL152_05120"
     CDS             9712..>11256
                     /locus_tag="PL152_05120"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQR48694.1"
                     /translation="MPQQTAESAAVQAAAPAAVAPRPGPNPLRDAFFGDLHLHTGYSM
                     DAFAFGTTLTPDDAYRYAEGATVTYFGKPQKRHAPLDFLAVTDHAEYLGVVRDTIDPN
                     GPFAGTEWYTMMTNRDPAVSAQAFKKLIGSTVTNQPLPELNDRKLLASAWQEYMAAAE
                     RHNQPGKFTTFVGFEWTSAPQFQNLHRCVIFAGKGPELPFSAFDSLDPEALWKYMEAQ
                     RALGLEVIAVPHNGNASNGLMFNVRDMSGRPLTREYAERRMANEPLVEIIQGKGQSDT
                     SPELSPNDEFADFEMWRTLVGNPAKAKALTGSYVRQAYGVGQELQEKLGVNPFKYGIE
                     AGTDFHSAMSSTEASNYPGSHGNQDIDPKSVITATESVGGEPPTAIGSGGLTGVWAEE
                     NTRASLFAAFKRKETFGTSGTRIKVRMFAGWDYPKGLVQQKDWVKAAYAGGVPMGGDL
                     APKAGSAAPTLLVQATKDPDGGNLDRIQIIKVATRHGKSTETVYDVVWSGERKPDPKT
                     GKVPAVG"
BASE COUNT         1689 a         3840 c         3864 g         1863 t
ORIGIN      
        1 tcgcgctcgg cctggccaac atggcgcaca tggagccggt cgggcacctc gggacggtcg
       61 cggcgctcgc gctgctcgtc gcgttcagct ccgcgacgca ggacattgcg gtcgacgcgt
      121 ggcgcatcga ggccgcgccc ccactcatgc agggcgccat ggccgcctcc taccagctcg
      181 gttatcgcat cgcgctcatg gtggcgagcg cgggcgcgct ctggatcgcg gcggatctcg
      241 gctgggacac cgcgtacacc gcgatggccg ctctcgtcgg catcggcctc gtgacgacgc
      301 tcgtgatccc cgagccggac gcgcgcatcc cgcgcgactc gctggaccag gaggctcgcg
      361 tcgtcgcctg gctcgagcgc aaggcacact ggccggagcc gctcaggcgc gtgggggcct
      421 ggttcacggg cgcggtcgtc tgcccgttcg tcgatttctt ccagcgctac ggcacgcgcc
      481 ttgcgatcct catgctggcg ttcatcgcga cgtaccggct cgccgacttc acgatgggcg
      541 tcatgaccaa ccccttctac ctggacatgg ggttctcgct gaaggagatc gccgcggtcg
      601 ccaagggctt cggcgtgttc atgtcgatcc tcggcacgct cgtcggcggc gtggccgtgg
      661 tgcgcctcgg gctcgtgcgc acgctcgtgc tcggctgctc cctgatcatc gccgcgaacc
      721 tgatgttcgc cgtcttcgcc tggacgggga agccgagtct cgcgggtctg gccgtcgtca
      781 tcagtgccga caacgtggcg atgggcatcg ccggcaccgc gctgatcgcg tacctgtcga
      841 gcctgacgag cgcgtcctac accgcgacgc aatacgccct gttctcgtcc atgtacgcgc
      901 tgcccggcaa gctcctgatg ggagcgtccg ggttcgtggt ggacgcgatc ggctatccgg
      961 cgttcttcgt ctacaccgcg acgctcgggg tgccggcgct ggtcatgctg tacatcgtga
     1021 cctgcactgc gaagcgcgcg gcgccggccg cggtgggcgc ggcgtaatgg aagagacgga
     1081 cctcgagcgc gtgatctgcc cgctgcacga gcttgcggac ccgggcgcga gggaattcac
     1141 gatgggcaac ggcgactggc cgttgcgcgg cttcgtggtg cgcagcgggg agaccgtgcg
     1201 cgcctacgtc aaccactgcc cgcacgcagg atttccgctc aactggactc cggacggatt
     1261 cctcgcgccg cactcgaggc tcatcctctg ctcgatgcac ggcgcggtct tcgagatcga
     1321 gacggggttg tgcatctccg gcccgtgcga cgggctcagg ctgcgtgcgc tgccggtgcg
     1381 cgtggagcgc ggttacgtga tgctgggcga cgaggtcccg ctcgaggagc cgcccgatct
     1441 cggaagctga agatgccgca ggggccgggc gctcagtagc gctcgccttc ctcggcctcc
     1501 ggcaggtcga cctcggagta accagcggcc tcgccgcggt ccacgaactg cagcggatcg
     1561 gcccaggcct tcgcggtgcc gggttcggcc tcgtattcgt tctcggtgtc ctcgggctcg
     1621 acgtcgagcg agcccgtcca gtcctcgggc ggctcggtgg actcgaactc gtcctcgagc
     1681 cactcgtcga cctcgagttc ctcgaccgtg ccgtcgaagt actggatctc gatcgtgcga
     1741 tcgtcgcggt cgatggccac gacctcgaag gaatctccgc cggggcggcg gtaccactgt
     1801 ccgatgacgg gtgcgagctt cgccatggtc ccaacctctg ccgatcgcgg tgcgtgtatc
     1861 ctctgctcgt cttcacctcc cttctagacc tctgcgaagg ggagttcaag tagcggaatg
     1921 cgcggactgc caatgttcga cgaactggtc aaattcttcg ttgtcctgct ggtcgtcgtc
     1981 gagccgctgt cgctgctgcc ggtcctcgcg ggcctgacgg acggcgcgga cgacggctac
     2041 cgccgccgca tgtcggtgaa ggcggtcggg atctccgtgg ccgtgtgcct ggtgttcgcg
     2101 ctcggcggca gcgcattgct gcaggtgctc ggcatcacgg tggatgcctt caaggtcggc
     2161 ggcggcatcc tgctgttcct gctcgccgtg gagatggtgt tcgcgaggcc ttccggcgtg
     2221 cggtccatca cgccgggcga ggacgccgag gcgcaccacc gcggcgacat ctcggtgttt
     2281 cccctggcat ttcccttgat cgccggcccg ggcgcgctgg cgaccatcct gctcgcattc
     2341 gccggggtcg agcccggatc gctccagttc gcggcccagg tgggcatcat cctggtcgtg
     2401 ctcgggatca ccctcgcgct catgctgctc accgggccgg tgatgcgcgt gatcggcgtg
     2461 accggtggcg ccgtgctcgg gcgcctgctc ggcgtgctgc tcgcggcgct ggcctctcag
     2521 ttcgtgctcg acggcatccg cggcgctatc gcttcctgaa gcagaggtcc cagacctcgt
     2581 ggccgaggcg ctggccgcgg ctctcgaagc gcgtgagcgg ccggctgtcc ggccgcgggc
     2641 agtatccatc gaactcgttg gcgagcgacc cgcagccgcc ggccaccgcg agcatctgct
     2701 ccgcgtagtg ctgccagtcg gtcgcgagcc ggaagcgtcc gccgggcctg agcttccgcg
     2761 ccaccaggtc cacgaacgcg ggttgcacga tgcgccgctt gtgatgccgt ttcttcggcc
     2821 aggggtccgg aaagtagagc agcacggtgt cgagcgacgc gtcggcaatc ctgcgctcga
     2881 gcacctcgac cgcatcgcgg cacgcgacac gcaggttgga tagcccgagc gcgccggcga
     2941 gcagcagcag gtgcccgaca cccgggcgat gcacctcgat gccgacgaag tcctcgccgg
     3001 ggtgctccgc ggccagggag accagcgatt cgccgttgcc gaagccgatc tcgagcgtga
     3061 ccggggcggt cctgccgaac agggccgcga aatcgagccc gccgtcgccg tcgtcgatcc
     3121 cgtatcgcgg ccacagctcg cgccaggcgc gctcctgggc gacggtcatg cgaccagccc
     3181 gcgcgacgaa actgcggatg gcgcgtcgcg ggcgctccgg cggtccttcg gcctgcgtgt
     3241 cgtctggaat cgtcggcgcc aagctactac agtccacagt cggggcagtg gctcgcatct
     3301 tacggcgctt gcaggcgccc gggagtactc acatggccag cacgaccgga atgctcgaca
     3361 acctcggtgc caatgcgcac gcgctctgcc ggcgcacgtg gtgggtgttc ctggtcggcg
     3421 gcctcgcgtc ggtcgcattc ggcgtgctgg cgttcgcgaa ccccgggctt gcgctgttcg
     3481 tgctggcggc gttcttcgcc gcgtcgatcc tggtggacgg cgcgttcaac atcctgggcg
     3541 ccctgcagca ccgggagaag gacgggtggt gggtcatgct gctcatgggg ctgctcggcg
     3601 tgctcgtcgg gggctacgcg ctgtggcatc cgccgatcag catgctcgcg ctggtctaca
     3661 tcgtcgcgtt ccaggcgatc atgctcggcg tgttcctggt tacgctggga tacaaggtcc
     3721 gcaaggcgac gacgcgcgag tggatcctgt acgccgcggg cggcctgtcg atcctgttcg
     3781 gcgtgctggt catcgtcaat ccgctcgccg gcgcggcgtc gatcgtctac ctgatcgcgt
     3841 gctgggcggt ggtcgtcggc gcgctgaagg tctggttcgg attcaaggtg aagagcctgc
     3901 ccacgaagct cggcgaccgg atgtcggccc tgcactgagc aggcgcgtca ggcgacgcgc
     3961 cccgcgatgc gcccgccgaa caggcagccg ccgaggaacg tgccctcgag accgcgcttg
     4021 ccgttcatgc cgccgccacc gaaacccgcg gattcgcccg cggcgtagag cccggggatc
     4081 ggctgtcccg atccgtcgag cacgcggctc gacaggtcgg tctgcaggcc gcccatgctc
     4141 ttgcggctga tgatgaactc gcgaatcgca agcaggggtc ccgccgctgg ctcgaggatc
     4201 ttctggaact tgcaggtgcg caggcggtcg ccgatccagc gacgcgcgaa ctcgatgcgg
     4261 cgcagctggt cgtcgtcgtg gaagcgcggg ccgaggtcga tgcgcgcatc gtacgtcgtc
     4321 gcggcctcgg tgatggcggc gaggtccacg gagcggtcgc cggtgagcgc attcatcttc
     4381 tcgaccagtt ccggcagcgt gtcggcgacc acgaagtcct tgcagttcgt cgtgacctcg
     4441 tcgtagagcc agcggttgcc gcgcagcatg tcgcgcgcga cgtggagccg cttcttctcc
     4501 cggaacgacg ggttgaactc gccgcccgag atcgcgagct ccttgagcgc gatcctgcgg
     4561 ttcagcagct gccacgagta cgcacgctcc tgccggcaga tctgcgcgac gaggtcgtgc
     4621 gtgtcgtagc cggtcacgag cggcatcggg ccgatgcgct cgccgcgcca gttgagccag
     4681 agcgcagact tgggcggcac gagcgagagc ccgtgcgagg gcttgcgcgg cttccagtgg
     4741 tgcacgcccg ccgcgtagtt ccactgccag tcgaggtgcg tgatgcgcgc gcccagcgtc
     4801 gacgcggcgt cgtgcaggcg tccgtccgcg tacttgtgcg agccgttcag gatcgtctcc
     4861 ggcggcttgc cccagtccgc gtgccagttc gccttgacgc gctcgaggtt gccgttgatg
     4921 ccgccggacg cgatgacgac cgactcgccg ctcgcctcga attcctgcgc cgaaccctcc
     4981 atcacgccgc gacagccgga gacgctgccg ttggtgacga cgagggactc gacgcgatgg
     5041 ccgaagcgca gctcgagcag cgggcggcgg gggtgggcga gcaggtgacg attcaacacc
     5101 acggccagtt cgtgcccggt tccccacacg atgtgccagc gcggcacgga gttgccgggc
     5161 gtgtacagtc cgcgctcgac ccacagcggc atgggcatga attcgacgcc gatgcccctg
     5221 agccagtcga agatctcctc gttcgcgcgc tgcacgtagc tttcggccca ggcgagcggc
     5281 cagcggtcgt ccggcccgaa ttccgcgaac gccttccagt cgcgcagtcc ctgctcgacg
     5341 ccgtcgcgga tcccgtagcg gcgctgcagc ggcgtgccgg cgaaccagat gccgccgaag
     5401 ctctccttgg cgaggccgcc catgttctcc tcggcgtcgc ggtcgatgag cagcacgcgc
     5461 ctcccgcgtc cgagcagctc gatcgcggcg gcgacaccgg cgatgcccga cccgatcacg
     5521 acgacgtcgg ccttgtaacg cagcacggtt cagtccccct ccgccagtgt caggtccacg
     5581 ccgtcgagcg tctcggcgga tccgaccccc atctgtttca cgagcttcgc cgccatgggc
     5641 atgccgatgt tgagcgggcg cacgcgcgag gccctcgcgt acacgacatt tccggacatg
     5701 ggcgtaggcg actgcggtat gaacacggcg gtccagcccg cgggcatggg ctccggcagc
     5761 gcgtagccga gctgccaggc gtcgtccagg cacacgagca cgggggcgag cccttgtccc
     5821 tgctcgacct tggtcagtcc ttcggccatc gtggtcacga cccggtattg cggcaggttg
     5881 ccgagcaatg agtccccgat ccatttcgtc attgcgcgtc cggtgttcgt gcgcgcgagg
     5941 agccccgcca cgaatgccaa gacgaggagc accagcgcgg cgacgatcgt gccgacggcg
     6001 accccggcga actggtgcgc cgggaacagc gctgcaatcg ggtccgcgac cttgcgggcc
     6061 atctccaatg ccttgcgcag caacaatgcc aggacgacga gcggcacgag aaataccagg
     6121 ccgccgatga ccgtggtctt gaggaattcc ttgatcctgt ccaccgcgac gctcccgtgg
     6181 aaccttcccg ccagaatacg cctctatcga tccgcggcca cctcgccgag caaccacggg
     6241 cgggctcggt cgcgatacga gtccgggatt cgcgccgggc ccgtcgaatc ggcaaagacc
     6301 atgaccgcgt ccgagacgct gacgcaacgg ccatcctgga acatgcccat ggcgagcgtg
     6361 tacgaggtgt tcccgagccg caggacgccg acgccgactt ccagcgtgcc ggggtagccc
     6421 gcttcgagca ggtagtcgat ggactggtgc gcgatgagga tgcgctcgcc cgccggcgtg
     6481 gggatgcgcg ctcccccgag catatgcgtc agggccgaca cccgcgcctc ctcgtaatat
     6541 tgggcgatcg cgacgttgtt gatgtgccac agcgggtcca cgtccgcgta acgggcgatg
     6601 atgcggacct tgagcggata gcgcgtcggg tccaggcgat ggggagcgat gcgggtcatg
     6661 ggcggattga cgtgtcccgg cggtgaccga atgatacggc gacgcccatg accgcagtcc
     6721 agttccggaa taccgaagtg cggctgtccg aggcactcgc ggccacggcc gcggccgtgc
     6781 gcgggcacgt cgtgacgctg cgggaactgc tcggctacgt cggcgagcag ggactgctcg
     6841 tcttcagcgc catcctcgcg ttgccgttcc tgatcccggt gtcgctgccg ttcatgagca
     6901 ccgccctcgg cctgccgatg ctgctgatcg gcattgcggt catcgcgggc cgcctgccct
     6961 ggctgccggc ccggcttctc gaccacgaac tgccgtccga gaccgtgcag cacctgctcg
     7021 aacgcgcgac ccggatggcg ctgcgcttcg agcacctcgt gaagccgcgg ctgccggtgc
     7081 tgaccggctc ggggttcgtg aactccctgc acggcgtgac gctcgtgacg gcggtgctgc
     7141 tgctgatggc gccgctgccg ttcgtgccgc tcgccaacac gctgcccggc atcgccatca
     7201 tcctgctgtg cctcggtatc gccgagcgcg acgggctgtt gctcgtggtc ggctatttcg
     7261 tcgcactcgt gtcggcggtg tacatcggct cgttgatgtg gctggtcgtc caggcgggca
     7321 tgaacttcga cgacgtgctg gccgagccag gagcctggtt ctcgcgaatc ttcaaggact
     7381 gactcgcggc caccctgctc gccgcggcgc tgctcccggg cacgcgcccc cccgaaactg
     7441 accctataat gcgcgccttc ggccgcggtc gacgtccgtc acgcgcggca ctgccattcg
     7501 aggtgattgc atgaagatct ggtccatgct ctcgaccatc gccgtcgcgc tgctgctcgc
     7561 ggcaggcgtc gcggacgcgg cgggcacccg tccgttcacg ccgaaggacc tcgtgatgct
     7621 cgatcgcgcg tcggagccgc ggctctcgcc ggacggcaag gtcctcgcct acacgctgcg
     7681 cgagacggat ctcgacgcca accgtggggc caggagcatc tggcgactgg atctcgccga
     7741 ccagaatgcg cacccgcagc gtgtgaccgc cgctggctcg aatgcgtggt cgccgcgctg
     7801 gtcacccgac ggccggacgc tgtatttcct ctccgaccgc gcagggagcg accaggtctg
     7861 ggcgttgaca tccgggctgg gtgacgcgcg ccaggtgacg aagctgccgc tcgacgtcgg
     7921 cagcttcgtg gtcgccccgg atggccggcg gctcgcggtc tcgctcgagg tcttcccgga
     7981 ctgcgcggat ctcgcctgca cgaagcagcg tcgggacgac cgcgcggcgg cgaaggtcag
     8041 cggcgtcgtc tacgacaagc tcttcatccg gcactgggac gcctggtccg acggcagtcg
     8101 ctcgcagctg ttcatcgcgg acgtcggtgc ggacggagtc gtctcgcagg agcctcggct
     8161 catcacgcgc ggcatcgacg gcgacgtccc gtcgaagccc ttcggcgacg acagcgagta
     8221 cgccttctcg cccgacggca agacggtcgt cttcaacgcg cgcatcgccg gcacgaccga
     8281 ggcgtggtcg acgaacttcg acatctactc ggtggcgacc gacggcgacg ccgcgccgcg
     8341 caatctcacc tccgggaatg cggcgtggga cggctatccg ctgccctccc cggacggccg
     8401 caaggtctat tacctggcga tgcgcaagcc catgcacgag gcggaccgct tcgggatcat
     8461 ggaactcgac ctcgcgaccg gacgcagccg cgagatcgct ccggactggg accgcagcgc
     8521 cggaccgctc aagatttccg cggacggccg caccctctat acgtcggcgg acgacgacgg
     8581 ccggcatcct ctctttgcgg tcgacgtggc cagcggcagg gtgaggaccg tcgcccaggg
     8641 tggcgacgtg acggacttca gcctcgccgc cggcacgatc gtcgtggccc gggcctcgct
     8701 caaggcgccc accgatttct atcgtgtcgc acccggcgcc gagcgacggc tgaccgacct
     8761 caacgctgcg cgcctgcgcg acgtcgtcat gggcgacgtg gaggtattcg agttcaaggg
     8821 ttggaacgac gagcgcgtgc gcggctatgt cgtcaagccc tacggcttcg agcctgggaa
     8881 gaaatacccg gtcgcattcc tgatccacgg cgggccggaa ggctcgtggc tggacgaatt
     8941 ccactatcgc tggaatccgc agacgtatgc gggcgcggga ttcgccgtgg tcgcgatcga
     9001 cttccacgga tcgacgggct acggccaggc gttcacggaa gccatcctcg agcacgcggg
     9061 cgaccggccg ttcgaggacc tgcagaaagg ctgggccgcg gcgctggcga agttcgattt
     9121 cctggatgcc ggccgcgcct gcgcgctggg cgcgagctac ggcggctaca tggtcaactg
     9181 gatcgcaggc aactggcagg cgccgtggaa gtgcctcgtg agccacgacg gcatcttcga
     9241 cagtcgctcc atgtactaca cgaccgagga aatctggttc gacgagacgg aacacggtgc
     9301 gccacagtat gaacggccgc aggcttacga gcagttcaac ccgatcaacc acgtcgcaga
     9361 gtggaaggtg ccgatgctcg tggtgcacgg ggcgaaggac taccggatcc cgctggagca
     9421 gggcatcggc gcgttcacgg cgctgcagcg gcgtggcatc ccgagccagt tcctgacctt
     9481 cccggacgag aaccactggg tgctcaagcc gcagaacagc ctcaagtggc acgagaccgt
     9541 cgaggcctgg atcaagcgct ggaccgcgcc ttaggcacac ccggcagctc cgctgactct
     9601 gcgtcaactg ttccggacag aggaccactc atgcaggcca ctcacgcccg cgcagtcttc
     9661 gtcgctacat tgatggccct gcttgctggc tgtgccggaa agaccggaga cgtgccgcag
     9721 cagaccgcgg agagcgccgc cgtgcaggcc gccgcgcccg ccgctgttgc gccccgtccg
     9781 ggcccgaatc ccctgcgcga cgctttcttc ggcgacctgc acctgcacac cggttattcg
     9841 atggatgcgt tcgcgttcgg cacgacgttg acgccagacg acgcgtaccg gtacgcggaa
     9901 ggagcgaccg tcacctactt cggcaagccg cagaagcggc atgcgccgct cgatttcctc
     9961 gcggtcaccg accatgccga gtacctcggc gtggtgaggg acacgatcga cccgaatggg
    10021 ccgttcgcgg ggaccgagtg gtacacgatg atgaccaacc gggacccggc ggtctcggcg
    10081 caggcgttca aaaaactcat cggctcgacg gtgacgaacc aaccgctgcc ggagttgaac
    10141 gatcgcaagc tgctggcgag cgcctggcag gagtacatgg cggcggccga gcgccacaac
    10201 cagccgggaa aattcaccac gttcgtgggc ttcgagtgga cctccgcccc gcagttccag
    10261 aacctgcacc gctgcgtgat cttcgccggc aaggggccgg agctgccgtt cagcgccttc
    10321 gactcgctcg atccagaagc gctgtggaag tacatggagg cacagcgcgc cctaggcctc
    10381 gaagtgatcg cggtgccgca caacggcaac gcgagcaatg gcctgatgtt caacgtgcgc
    10441 gacatgtcgg gccggccgct gacgcgcgaa tacgccgagc ggcgcatggc gaacgagccg
    10501 ctcgtcgaga tcatccaggg caagggccag agcgacacca gcccggagct gtcaccgaac
    10561 gacgagttcg cggacttcga gatgtggcgc accctcgtcg gcaatccggc caaggccaag
    10621 gcgctgaccg gcagttacgt gcgccaggcc tacggcgtgg gccaggagct gcaggagaag
    10681 ctcggggtca atccgttcaa gtacggcatc gaggccggca cggatttcca ctcggccatg
    10741 tcgagcaccg aagcgagcaa ctacccgggc tcgcacggta accaggacat cgatcccaag
    10801 tcggtcatca cggcgacgga atccgtcggt ggcgagccgc cgaccgcgat cgggtcgggc
    10861 ggactgacag gcgtgtgggc ggaggagaac acccgcgcat cgctgttcgc ggctttcaag
    10921 cgcaaggaga ccttcggcac gtcgggcacg cgcatcaagg tgcgcatgtt cgcaggctgg
    10981 gactacccga aaggcctcgt gcagcagaag gactgggtca aggccgccta cgcgggcggc
    11041 gtgccgatgg gcggcgacct tgcgcccaag gctggctccg ctgcgccgac gttgctggtc
    11101 caggccacga aggacccgga cggcggcaac ctcgaccgca tccagatcat caaggtcgcg
    11161 acgcggcacg ggaagagcac ggagacggtg tacgacgtcg tgtggagcgg cgagcgcaag
    11221 ccggatccga agaccggcaa ggtccccgcg gtcggc
//
LOCUS       DAOMFD010000058        10976 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1617_length_10976_cov_36.238623, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000058 DAOMFD010000000
VERSION     DAOMFD010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10976)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10976)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10976
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1617_length_10976_cov_36.238623"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..734)
                     /locus_tag="PL152_05125"
     CDS             complement(<1..734)
                     /locus_tag="PL152_05125"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HQR48695.1"
                     /translation="MSFFTNLKADRLIADIKAASEGSGVVDATKALDRLGKLGPQAIP
                     RIIDALSAADRQEMDAFIGVLATLIDNKSLGTVVGALTDANQRTAQAIVTALSMGREY
                     NPQLLLPLLDDPKAPRGPIVNVLAAQKSRLSARDLLKRAYNQEATEKAALFRIIGDIA
                     DEGLLPELISRLEGKDPIARTHIINILARFNRPAVAAALQTQLKDPNKLIRQAVLNAL
                     SRLDGNADPAILCDLLRDPDVETQQKA"
     gene            944..2035
                     /gene="apbC"
                     /locus_tag="PL152_05130"
     CDS             944..2035
                     /gene="apbC"
                     /locus_tag="PL152_05130"
                     /inference="COORDINATES: protein motif:HMM:NF008669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HQR48696.1"
                     /translation="MTDDRVREARLVVGNYVEPHLGMPLADAKAVKSVRFEDGRVRVE
                     IELGFPLERGRVALQEHLGRLLAGTPKAGPVEVTVTCKVAAQPVEGAKPPLPGISNVI
                     AVASGKGGVGKSTTAVNLALALALDGARVGLLDADIYGPSQPRMMGLQGQRPVSTDGR
                     RMEPLVSHGVKVMSIGFLVEEEQPMVWRGPMVTQALTQLLAETNWGELDYLVVDMPPG
                     TGDIQLTLAQRVPVSGAVVVTTPQDIALLDARKGLRMFQKVAVPVLGIVENMGTHVCS
                     KCGHEEPIFGSGGGRAMAEQYGVALLASLPLDIRIREQTDGGCPTVVAEPDGPLGRAY
                     LEMARCTAARLSVAHSAAGAAPRITVEDT"
     gene            2038..2604
                     /gene="dcd"
                     /locus_tag="PL152_05135"
     CDS             2038..2604
                     /gene="dcd"
                     /locus_tag="PL152_05135"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004922407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HQR48697.1"
                     /translation="MSIKSDRWIRRMAEAHGMIEPFEPGQVRVANGNKIVSFGTSSYG
                     YDVRCAAEFKIFTNINSTIVDPKDFDHGSFVDFTGDVCIIPPNSFALARTVEYFRIPR
                     NVLTVCLGKSTYARCGIIVNVTPLEPEWEGHVTLEFSNTTTLPAKIYANEGVAQMLFF
                     ESDEVCEVSYKDRGGKYQGQRGVTLPKA"
     gene            complement(2656..3474)
                     /locus_tag="PL152_05140"
     CDS             complement(2656..3474)
                     /locus_tag="PL152_05140"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HQR48698.1"
                     /translation="MRSRTNLLRAALGLALAIQCVPVAWSQTGTASPAASQPRPQAAV
                     APASPAPAASGLTRYTARYQVSYRGISGGELEQSLHPGAVAGQWVYESRAYPNLLGRI
                     AVSPDARQRSTMELTASGVRPLTLVFDDGSDTSAKDVNIAFDWATGHVHGQAKQKTFD
                     YPVAAGTQDSASVQAAMINELLAGREPKSFWILNGAKLQEFRYWPEGRATVVTPYGQF
                     DTVVWASQHPGSNRLNRVWHAPQLGYVPVQAIQYNKGRPDVQLKLVTLDRGRSP"
     gene            complement(3458..4132)
                     /gene="purN"
                     /locus_tag="PL152_05145"
     CDS             complement(3458..4132)
                     /gene="purN"
                     /locus_tag="PL152_05145"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HQR48699.1"
                     /translation="MSASVARMPVVALISGRGSNLRAIAERARTGDPPIDIRAVVSDR
                     EDAPGLQWARSQGIDCATICPRSFPDRARYDAALAALVAGYEPGLVILAGFMRILGPA
                     FVDRYAGRTLNIHPSLLPKFRGLHTHRKALEAGERVHGASVHFVTRELDGGPVVIQAK
                     VPVRDDDDEASLAARVLEQEHRIYPQAVGWFATGRLRCIDDAAWLDGRRLDAPVLLDT
                     SGAQPD"
     gene            complement(4129..5163)
                     /gene="purM"
                     /locus_tag="PL152_05150"
     CDS             complement(4129..5163)
                     /gene="purM"
                     /locus_tag="PL152_05150"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HQR48700.1"
                     /translation="MADLRPTTYRDAGVDIDAGDELVERIKPAVKRSMRPEVMGGIGG
                     FGALVEVPLHRYRQPVMVSGTDGVGTKLRLAIETGRHDTIGIDLVAMCANDVVVQGAE
                     PIFFLDYYATGKLQVPVAERVIKGIVEGCLQAGCALVGGETAEMPGMYEGDDYDLAGF
                     CVGLVEKDRIIDGTRTRAGDVIIGLASSGPHSNGYSLIRRLLETGGAGPGTLLDGRPL
                     YDLLLAPTRIYVKSVLALLAELPVHSIAHITGGGPTGNIPRVLPDGLEAVLEERAWHR
                     PPVFDWLQRTGSVERDEMHRTFNCGLGMTICVAAADADGALAILRRCGETATVVGEVR
                     RGGRGIVIER"
     gene            5259..6254
                     /locus_tag="PL152_05155"
     CDS             5259..6254
                     /locus_tag="PL152_05155"
                     /inference="COORDINATES: protein motif:HMM:NF021367.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2066 domain-containing protein"
                     /protein_id="HQR48701.1"
                     /translation="MLRQPRTTTPGGAALRVVACVLFLAASLRAGADVAVYQAVVPLA
                     GTTEADRNAAFGDALRTAAVRATGQRDAGSAPAVAAAAADPTRYVQQYSTTPDRMLKV
                     GFDARAMDRLLQQAGLPFWPLERPVTLVLLVVPSVAGGQRAVLASERVVPERVELERA
                     ALARGLPIAWPEQPVSAAQVRAAMGGDDGAVGDSQYVLAGVGSGGSIAWRFTGVGESA
                     RVDGAPRDGADLAADALAARYAPASSRGTSTVAIRVGGIADVRSYASLLEYLKSLSLV
                     RAVGVSGLDDREVLLDVTLRGDLGQLRRIAALDSHLKPAAATQGEVTAADFTYQP"
     gene            6251..6820
                     /locus_tag="PL152_05160"
     CDS             6251..6820
                     /locus_tag="PL152_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQR48702.1"
                     /translation="MSLRHLPNLLCVLRMLLVYPVGHGILDGHYPQVLALFALAAFTD
                     GLDGFLAKRYGWTTELGGHLDPLADKLLLVTVFVCLSVAELAPWWLTATVLLRDVVIV
                     SGAIAYRIVVGPFRGLPTLQSKFNTFCQILFCLLVVAQAAYEWPLDDVVTAAGALAFV
                     TTSVSGLDYVLAYSRRSVAAVRGRATRPG"
     gene            6827..7525
                     /gene="hda"
                     /locus_tag="PL152_05165"
     CDS             6827..7525
                     /gene="hda"
                     /locus_tag="PL152_05165"
                     /inference="COORDINATES: protein motif:HMM:TIGR03420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0032297 - negative regulation of
                     DNA-templated DNA replication initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA regulatory inactivator Hda"
                     /protein_id="HQR48703.1"
                     /translation="MRQLPLGVRLRDTARLESYVPGGNREAVELVAGLRLPAPRVLWI
                     WGRRGTGKTHLLQAACASVADRGGSASYLDPAAGAEPDWLEGCEGLDLVCIDALDAIS
                     GDAPWNAAIFRLHTLMQDAAARLCVATERAPATVDFVLPDLRSRLLAGSVHQLQELDD
                     AGLCEALRLRAAQRGLELSQEGALYLLHRLPRDMHTLCEALDRLDEASLAAQRRLTVP
                     FLRGALAGQGLGDD"
     gene            complement(7504..7884)
                     /locus_tag="PL152_05170"
     CDS             complement(7504..7884)
                     /locus_tag="PL152_05170"
                     /inference="COORDINATES: protein motif:HMM:NF021369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2069 domain-containing protein"
                     /protein_id="HQR48704.1"
                     /translation="MAASLDRRARLVRRCALAALVALASFSAAAVLMPWSGVPSAVLG
                     ALLLPPLLMPLRGMLRGDRRTYAWATLCAVPGFVYGITEAVANPAMRWLAALVLGSSI
                     AFFLALVAFLRVTRPRPGQSSPSP"
     gene            complement(7887..8489)
                     /gene="wrbA"
                     /locus_tag="PL152_05175"
     CDS             complement(7887..8489)
                     /gene="wrbA"
                     /locus_tag="PL152_05175"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003955 - NAD(P)H dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HQR48705.1"
                     /translation="MTEVLVLYYSRNGSTAELARHVARGVESVEGATARVRTVPPVAP
                     VTLQAEPAVPESGAPYATHEDLRECDGLVMGSPTRFGNMAAPLKYFLDGTASLWLNGA
                     LDRKPAGVFTSTSSMHGGQESTLLAMMLPLLHHGMVIVGLPFTAEAMNRTRTGGTPYG
                     ASHLAARKTETPLSDDERELAQLLGRRVAEAAVALAGVRR"
     gene            8601..8969
                     /locus_tag="PL152_05180"
     CDS             8601..8969
                     /locus_tag="PL152_05180"
                     /inference="COORDINATES: protein motif:HMM:NF025704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4398 domain-containing protein"
                     /protein_id="HQR48706.1"
                     /translation="MKSPARLFLSVATTALFGAALASGCVGWPVQEMSNARQAIVAAQ
                     KAGAGEHAPDTLAQAQKLLADAKESANHGDYRAARDQAEQAREKAIEARKAAEAAKAP
                     KPATSGGPDPTPQAGAEPHP"
     gene            8974..9261
                     /locus_tag="PL152_05185"
     CDS             8974..9261
                     /locus_tag="PL152_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HQR48707.1"
                     /translation="MTAWIARRCLVAGRVQGVFYRASTRQRAAELGVAGHARNLADGR
                     VEVLACGDPQAVRALCDWLWTGSPASHVTAVEVEEVDEAELRARPEGFRTA"
     gene            complement(9271..9846)
                     /locus_tag="PL152_05190"
     CDS             complement(9271..9846)
                     /locus_tag="PL152_05190"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HQR48708.1"
                     /translation="MRRSAPLLPLLLLAALPATAARADRQMDPELRGVIAGAIKPTTC
                     FEDRYEQAVWLAYMEPRLARYVKDTEEREQILHHVHCEARRVDIPPELVLAVMDVESR
                     FDRYAVSSAGAVGLMQVMPFWPTQLGMTNAQLVRVGDNVRMGTTILGYYLRVERGNYQ
                     RALQRYNGSLGRPDYSDLVIDRLVSRWRYAH"
     gene            complement(9849..>10976)
                     /gene="proS"
                     /locus_tag="PL152_05195"
     CDS             complement(9849..>10976)
                     /gene="proS"
                     /locus_tag="PL152_05195"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HQR48709.1"
                     /translation="ADTGSIGGSASQEFHVLAESGEDAIAFSDGDDYAANLEMAEALP
                     PAGGRPAPGAVLSRVATPNVRTIAELTAFLGIEASRCLKTLLVEGSDGGVVALIVRGD
                     HELNAVKAQKLPGVFSPLKMATAEQVREAAGCEAGFIGPVGLKLKVYADHAALNAADF
                     VCGANERDHHLTGVNWGRDLPEAEAADIRNVVPGDPSPSGRGRLGIARGIEVGHIFQL
                     GRKYSEAMNATVLDEQGKSVTMFMGCYGIGVTRVVAAAIEQNNDERGIVWPEPIAPFH
                     VSLIPINLQKSERVREVAERLYAELQAAGFEVLYDDRDARPGVKFADDELLGIPHRVV
                     VGDKGLERGVLEYKPRAGAGAGDVPVASLVEFLKERRNPRS"
BASE COUNT         1584 a         3796 c         3911 g         1685 t
ORIGIN      
        1 gccttctgct gggtctccac gtccgggtcg cgcagcaggt cgcagaggat cgccgggtcg
       61 gcgttgccgt cgaggcgcga cagcgcgttc agcaccgcct gccggatgag cttgttcggg
      121 tccttgagct gggtctgcag ggcggccgcc accgccgggc ggttgaagcg cgcaaggatg
      181 ttgatgatgt gcgtgcgcgc gatcgggtcc ttgccctcca gccggctgat gagctcgggc
      241 aggagaccct cgtcggcgat gtcgccgatg atgcggaaca gggcggcctt ctcggtcgct
      301 tcctggttgt aggcgcgctt cagcaggtcg cgcgccgaca gccgggactt ctgggcggcc
      361 agcacgttga cgatcgggcc gcgcggcgcc ttcggatcgt cgagcagggg gaggaggagc
      421 tgcgggttgt actcccgccc catcgacagc gccgtgacga tcgcctgcgc cgtgcgctgg
      481 ttggcgtcgg tcagcgcgcc gacgacggtg ccgagcgact tgttgtcgat gagcgtcgcg
      541 agcaccccga tgaaggcgtc catctcctgc cggtccgccg cggacaacgc gtcgatgatg
      601 cggggaatgg cctgcggccc gagcttgccg agccgatcga gcgccttggt ggcgtcgacg
      661 acgccgctcc cttcactcgc cgccttgatg tcggcgatga ggcggtcggc cttcaggttg
      721 gtgaagaagc tcatggacgc cggtcggttg cgctgggctt aggcctcgtc gtgcctgact
      781 aagtcaaagt gggggccgcc tgccgggagc acgacgaccc gccgcagtat gccatgtgtc
      841 cgcggtccgc ccgcggcgcc cggcggcacc gagccgcggg cggaattccg ttacaataac
      901 gcgtttacgg cgcaagccaa tctccctctg gtccggtcac ccaatgacag acgatcgcgt
      961 acgcgaagcc cgccttgtgg tcgggaatta tgtcgaaccc cacctcggga tgccgctggc
     1021 ggacgccaaa gctgtgaagt ccgtcaggtt cgaggatggc cgcgtccgcg tggagatcga
     1081 gctcgggttt ccgctggagc gcggccgggt cgcgctgcag gagcacctcg gacgcctgct
     1141 ggcgggcact cccaaggccg gtccagtgga ggtcaccgtc acgtgcaagg tggctgccca
     1201 accggtcgag ggggccaagc cgcctctgcc gggcatctcc aacgtgatcg cggtcgcctc
     1261 cggcaagggc ggcgtcggca agtccacgac cgcagtgaat ctcgcactgg cgctcgcgct
     1321 cgacggcgcg agggtcgggc tgctcgatgc ggacatctac ggccccagcc agccgcgcat
     1381 gatgggcctg caggggcagc ggccggtatc gaccgacggc cggcgtatgg agcctctcgt
     1441 gtcccacggc gtcaaggtca tgtcgatcgg cttcctcgtc gaggaggagc agccgatggt
     1501 gtggcgcggg cccatggtga cgcaggccct gacccagttg ctcgcggaga cgaactgggg
     1561 cgaactcgac tacctcgtcg tggacatgcc gccgggcacc ggagacatcc agctgacgct
     1621 cgcccagcgg gtgccggtga gcggcgcggt ggtggtcacg acgcctcagg acatcgcgct
     1681 gctcgacgcc cgcaagggcc tcaggatgtt ccagaaagtc gctgtcccgg tgctcggcat
     1741 cgtcgagaac atggggacgc acgtctgctc gaagtgcggc cacgaggaac cgatcttcgg
     1801 cagcggcggg ggtcgcgcca tggctgagca gtacggcgtc gcgctgctcg ccagcctgcc
     1861 gctcgacatc cgcatccgcg agcagaccga cggcgggtgc ccgacggtcg tcgccgagcc
     1921 cgacggcccg ctcgggcgtg cctatctgga gatggcccgc tgcacggccg cacggctgtc
     1981 ggtcgcgcat tccgccgccg gcgcggcccc caggatcacc gtcgaggaca cctgaccgtg
     2041 agcatcaaat ccgaccgctg gatccgccgc atggccgagg cgcacggcat gatcgaaccg
     2101 ttcgagcccg ggcaggtgcg cgtggcgaac ggcaacaaga tcgtgtcgtt cggcacgtcg
     2161 agctacggct acgacgtgcg ctgcgccgcc gagttcaaga tcttcaccaa catcaactcg
     2221 acgatcgtcg accccaagga cttcgaccac gggtcgttcg tcgacttcac gggcgacgtg
     2281 tgcatcatcc cgccgaactc gttcgcgctc gcgcgcacgg tcgagtactt ccgcatcccg
     2341 cgcaacgtgc tgaccgtgtg tctcggcaag tccacgtacg cgcgctgcgg catcatcgtg
     2401 aacgtcacgc cgctcgagcc ggaatgggag ggccacgtga cgctcgagtt ctccaacacc
     2461 acgacgctgc ccgcgaagat ctacgcgaac gagggcgtcg cgcagatgct gttcttcgaa
     2521 tcggatgagg tctgcgaggt cagctacaag gatcgcggcg gcaagtacca ggggcagcgg
     2581 ggagtgaccc tgccgaaggc ctgatgggga ctgtccccgc aggggactgt ccctggccgc
     2641 actggggaca gtcccctacg gggaccgtcc cctgtccagc gtcaccagct tgagctgcac
     2701 gtcgggacgt cccttgttgt actggatcgc ctgcaccggg acgtagccga gctgcggggc
     2761 gtgccagacc cggttcaacc ggttcgaccc tggatgctgg ctcgcccaca cgacggtgtc
     2821 gaactggccg tagggggtga cgacggtcgc gcggccttcc ggccagtagc ggaattcctg
     2881 cagcttcgcc ccgttgagga tccagaacga cttcggctcc cggcccgcca gcagctcgtt
     2941 gatcatcgcc gcctgcaccg acgcgctgtc ctgggtgccg gccgcgacgg gatagtcgaa
     3001 ggtcttctgc ttcgcctggc cgtggacgtg gccggtcgcc cagtcgaacg cgatgttgac
     3061 gtccttggcg ctcgtgtcgc tgccgtcgtc gaacaccagc gtgagcggcc gcacgccgga
     3121 ggccgtcaat tccatcgtgc tgcgctggcg tgcatccggg ctgaccgcga tgcggccgag
     3181 caggttcggg taggcccgcg actcgtagac ccattgcccg gcgacggcac ccgggtgcag
     3241 ggactgctcg agctcgccgc cgctgatgcc gcgataactc acctggtaac gcgcggtgta
     3301 tcgagtgagc ccggacgcgg ctggcgccgg cgacgcgggc gcgaccgcag cctggggtcg
     3361 gggttgcgat gcggccggcg acgccgtgcc ggtctgcgac cacgcgaccg ggacgcactg
     3421 gatcgcaagc gccaggccca gcgccgcgcg cagcaggtta gtccggctgc gcaccgctcg
     3481 tgtccagcaa gacgggcgcg tcgagccggc gcccgtcgag ccaggccgcg tcgtcgatgc
     3541 aacgcagccg gcccgtcgcg aaccagccga cggcctgggg atagatgcgg tgttcctgct
     3601 cgagcacgcg ggcggcaagg ctggcctcgt cgtcgtcgtc ccgcacgggg accttcgcct
     3661 gtatgaccac cgggccgccg tcgagttcgc gggtcacgaa gtggacgctg gcaccgtgca
     3721 cgcgctcgcc ggcctccaat gccttgcgat gcgtgtgcag gccgcggaac ttcggcagca
     3781 gggaagggtg aatgttgagc gtgcgccccg cgtagcggtc gacgaaggcg ggcccgagga
     3841 tgcgcatgaa gccggccagg atcacgagtc cgggctcgta gccggcgacc agcgccgcca
     3901 gggccgcgtc gtatcgcgcg cggtccggga agctcctggg acagatcgtc gcgcagtcga
     3961 ttccctggct gcgggcccac tggaggccgg gtgcatcctc ccggtcactc acgaccgcgc
     4021 ggatgtcgat cggagggtcg cccgtgcgag cccgctcggc gatggcccgc aggttgctgc
     4081 cgcggccgga gatgagggcc acgaccggca tgcgcgccac gctggcgctc atcgttcgat
     4141 cacgatgccg cgtccgccac gccgcacctc gcccacgacg gtcgcggtct cgccgcaacg
     4201 cctgaggatc gcgagggcgc cgtcggcgtc ggccgcggcg acgcagatcg tcatccccag
     4261 gccgcaattg aaggtgcgat gcatctcgtc gcgctcgacg ctgcccgtgc gttgcagcca
     4321 gtcgaacacc ggcggtcggt gccacgcgcg ctcctcgagc acggcctcga ggccgtcggg
     4381 cagcacgcgc gggatgttgc cggtcggccc gccgcccgtg atgtgtgcga tcgagtgcac
     4441 gggcagctcg gcgagcagcg cgagcaccga cttcacgtag atgcgggtcg gcgcgagcag
     4501 caggtcgtag agcggcctgc cgtcgagcag ggttcccggc cccgcaccgc ccgtctcgag
     4561 cagccggcgg atcagcgaat agccgttcga gtgcggtccc gacgaggcga gcccgatgat
     4621 cacgtcgccg gcgcgggtcc gggtgccgtc gatgatgcgg tccttctcga cgaggccgac
     4681 gcagaatccc gcgaggtcgt agtcgtcacc ctcgtacatg ccgggcatct cggctgtctc
     4741 gccgccgacc agcgcgcagc cggcctgcag gcagccctcg acgatgccct tgatcacgcg
     4801 ctcggcgacc ggcacctgca gcttgcccgt cgcgtagtag tcgaggaaga aaatcggctc
     4861 ggcgccctgg acgacgacgt cgttcgcgca catcgcgacg aggtcgatgc cgatcgtgtc
     4921 gtggcggccc gtctcgatgg ccagccgcag tttcgtcccg accccgtccg tacccgagac
     4981 catgaccggc tgccgatagc ggtgcagcgg cacctccacg agcgcgccaa acccgccgat
     5041 gccgcccatg acctcgggac gcatggaccg cttcaccgct ggcttgatgc gctcgacgag
     5101 ttcgtcgccc gcgtcgatgt cgacaccggc gtcgcgatag gtggtgggtc tcaagtcggc
     5161 catcgagtgg tctcccgcag ggcggttctg ccccggggca caggtcgagg ctgtgttatt
     5221 ttacacgtgc gccggcagcg ccggattctc gccgatccat gctgagacag ccacgcacca
     5281 cgacccctgg cggggcggcc ttacgggtcg tcgcctgtgt cctgttcctc gcagcgtcgc
     5341 tgcgggcggg ggccgacgtg gccgtttatc aggctgtcgt gccactggct ggcacgaccg
     5401 aagccgatcg aaacgccgcg ttcggcgatg cattgcgcac ggcggccgtg agggcgaccg
     5461 gccagcgcga cgcagggtcg gcgccggcgg tcgcggcagc ggccgccgac ccgacgcgct
     5521 acgtccagca gtattcgacc acgcctgacc gcatgctcaa ggtcgggttc gatgcccgcg
     5581 cgatggaccg tctcctgcag caggccgggc tgcccttctg gccgctggag cggccggtga
     5641 cgctcgtgct gctcgtcgtc ccgtcggtcg ctggcgggca gcgcgcggtg ctggcgtcgg
     5701 aacgggtggt gccggagcgg gtcgaactgg aacgggcagc cctcgcgcgc ggactcccga
     5761 ttgcttggcc ggagcagccc gtgtccgcgg cgcaggtgcg cgcggcgatg ggcggggacg
     5821 acggcgccgt cggcgacagc cagtacgtcc tggcgggcgt cggcagcggc ggatcgatcg
     5881 cgtggcgatt cacgggtgtc ggcgagtcgg cgcgggtcga cggcgcgcct cgggatggcg
     5941 cagacctcgc ggccgatgcg ctggcggcac gctatgcacc cgcatcgagt cgcggcacgt
     6001 cgaccgtcgc gatccgggtc ggcgggatcg cggacgtccg gtcatacgca agcctgctcg
     6061 agtacctgaa atcattgagt ctcgtgcgcg ccgtcggcgt atccggactg gacgaccggg
     6121 aagtgctcct cgacgtgacg ctgcgtggcg acctcgggca gttgcgcagg atcgcggctc
     6181 tggatagcca cctgaaaccg gcagcagcga cgcagggcga ggtgaccgcc gctgacttca
     6241 cgtaccagcc atgagcctgc ggcaccttcc gaacctgctg tgcgtcctgc ggatgctgct
     6301 cgtctacccg gtcgggcacg gcatcctgga cgggcactac ccgcaggtgc tggccctgtt
     6361 cgccctggcg gcgttcaccg atggactgga cggcttcctg gccaagcgct acggctggac
     6421 gacggaactc ggcggccatc tcgacccgct cgcggacaag ctgctcctcg tcacggtgtt
     6481 cgtgtgcctc agcgtggcgg aactcgcgcc gtggtggctg accgcgaccg tgctgctgcg
     6541 cgacgtcgtc atcgtgtcgg gcgcgatcgc gtaccgaatc gtggtcgggc cgttccgcgg
     6601 actgccgacg ctgcagagca agttcaacac cttctgccag atcctgttct gcctgctggt
     6661 cgtcgcgcaa gccgcctacg aatggccgct ggacgacgtg gtgaccgcgg ccggcgcgct
     6721 cgcattcgtc acgaccagcg tgagcggact cgactacgtg cttgcgtact cgcggcggtc
     6781 cgttgcggcg gtgcgcgggc gcgccaccag gccgggctga cgcacgatgc ggcagttacc
     6841 gctcggtgtg cgcctgcggg acacggcacg cctcgagagc tacgtcccgg gcgggaatcg
     6901 ggaggcggtc gaactcgtcg cagggcttcg gctcccggcg ccgcgcgtgc tgtggatctg
     6961 gggtcggcgc ggcaccggca agacgcatct cctgcaggcc gcctgtgcct ccgtcgccga
     7021 ccgcggcggc tcggccagtt acctggaccc ggcggcgggt gccgaacccg actggctgga
     7081 gggctgcgag ggcctcgacc tcgtgtgcat cgacgcattg gacgcgatct ccggggacgc
     7141 gccctggaac gccgcgattt tccgcctgca cacgctcatg caggacgcgg cggcccggct
     7201 gtgcgtggca acggagcgcg caccggcgac cgtcgatttc gtcctgcccg acctgcgctc
     7261 ccggctgctc gcggggtcgg tgcaccagtt gcaggaactg gatgacgccg gtctttgcga
     7321 agcgttgcgc ctccgcgctg cgcagcgcgg gctcgaactg tcgcaggagg gagcgctcta
     7381 cctgctgcac cgcctgccgc gcgacatgca cacgctctgc gaggcgctgg accggctcga
     7441 cgaggcgtca ctggcggccc agcggcgcct gacggtcccg ttcctgcggg gtgccctcgc
     7501 cggtcaggga ctcggagacg actgaccggg gcgcggtcgc gtgaccctca ggaacgccac
     7561 gagcgcaagg aagaacgcga tgctcgagcc caggaccagg gccgccagcc agcgcatggc
     7621 cgggttggca accgcctcgg tgatcccgta gacgaagccc ggcaccgcgc aaagcgtcgc
     7681 ccatgcgtag gtgcgccggt cgccccgcag catcccgcgc aacggcatga gcaggggcgg
     7741 cagcagcagc gcgccgagca ctgcggacgg gaccccggac catggcatga gcacggcggc
     7801 ggcgctgaac gacgccagcg caacgagcgc cgccagggca caccggcgga ccagcctggc
     7861 tcgccggtcg aggctcgctg ccatgctcag cggcgtacgc cagccagggc gacggccgcc
     7921 tcggccacgc gacggcccag caactgtgcc agttcgcgtt catcgtcgga gagcggtgtc
     7981 tcggtcttgc gtgcggcgag gtggctcgcg ccgtacgggg tcccgccggt gcgcgtccgg
     8041 ttcatcgcct cggccgtgaa cggcaggccg acgatgacca tgccgtggtg caggagcggc
     8101 agcatcatcg cgagcagggt cgattcctgg ccgccgtgca tcgaactcgt cgacgtgaat
     8161 acgccggccg gcttgcggtc cagcgcgccg ttcagccaca gcgaggcggt gccgtcgagg
     8221 aaatacttga gcggggccgc catgttgccg aagcgcgtcg ggctgcccat caccaggccg
     8281 tcgcactcgc gcaggtcctc gtgcgtcgcg taaggggcgc cggactcggg aaccgccggc
     8341 tcggcctgca gcgtaaccgg tgcaacgggt ggcaccgtgc gcacccgggc cgtggcgccc
     8401 tcgaccgatt ccacgccgcg ggccacgtgg cgcgcaagct ccgcggtgct gccgttgcgc
     8461 gagtaataga gcaccagcac ttcggtcatc ggggatcaga tcctctcggg ctcacacggg
     8521 gcgcgcggat tctaggtcgc ccgtccttga cggtacaggg tgcagttgtt aggttggttt
     8581 ttccagaccg tcacctgccc atgaaatccc cagcacgact gttcctgtcg gtagcgacga
     8641 ccgcactgtt cggcgccgcg ctggcgtcgg gctgcgtcgg ctggccggtg caggagatga
     8701 gcaatgcgcg ccaggcgatc gtagcggcgc agaaagccgg cgcaggcgag cacgccccgg
     8761 acacgctggc ccaggcccag aagctgctgg ccgacgcgaa agagagcgcc aatcacggcg
     8821 actatcgcgc ggcccgggac caggcggagc aggctcgcga gaaggcgatc gaggcccgca
     8881 aggcggccga ggcagccaag gcgcccaagc ccgccacgag cggcggcccc gacccgacac
     8941 cgcaagcggg cgccgaaccg cacccctgac cgaatgaccg cgtggatcgc gcgccgctgt
     9001 ctcgtcgccg ggcgcgtgca gggcgtcttc taccgggcca gcacccggca gcgcgccgcg
     9061 gaactcggcg tcgcgggcca cgcgcgcaat ctcgcggacg ggcgggtcga ggtcctcgcc
     9121 tgcggtgacc cgcaggcggt tcgtgcgctc tgcgactggc tgtggaccgg gtcgccggcc
     9181 tcgcacgtca cggcggtcga ggtcgaggag gtcgacgagg ccgaattgcg cgcccggccc
     9241 gagggcttcc ggaccgcctg agccgtcccg tcagtgcgcg taacgccagc gcgagaccag
     9301 gcggtcgatc acgaggtccg agtagtcggg ccgaccgagg ctgccgttgt aacgctgcag
     9361 ggcgcgctgg taattgccgc gctcgacccg caggtagtag ccgaggatcg tcgttcccat
     9421 gcgcacgttg tcgcccacgc gcaccagctg tgcgttggtc atgccgagct gcgtcggcca
     9481 gaacggcatg acctgcatca gccccacggc cccggccgac gaaaccgcgt agcggtcgaa
     9541 acggctctcg acgtccatga cggcgagcac gagttcgggc gggatgtcca cgcgccgcgc
     9601 ttcgcagtgg acgtggtgca ggatctgttc gcgctcctcc gtgtccttca cgtagcgggc
     9661 gagccgtggc tccatgtagg cgagccacac ggcctgctcg tagcggtcct cgaagcacgt
     9721 cgtgggcttg atcgccccgg cgatgacgcc gcgcagctcc gggtccatct gccggtccgc
     9781 gcgagctgcc gtcgccggca acgcggcgag cagcaggagc ggcagcagtg gcgcggatcg
     9841 gcgcatcttc agctccgcgg gttgcggcgc tccttcagga actcgacgag cgaggcgacc
     9901 ggcacgtcgc cggcgccagc ccctgcacga ggcttatatt cgagaactcc gcgctcgagg
     9961 cccttgtcgc cgacgaccac gcgatgcggg atgccgagca gctcgtcgtc ggcgaacttg
    10021 acgccgggac gggcgtcgcg gtcgtcgtac aggacctcga agccggccgc ctgcagctcg
    10081 gcgtagaggc gctcggcgac ctcgcgcacg cgctcggact tctgcaggtt gatcgggatc
    10141 agcgacacgt ggaagggtgc gatcggttcc ggccagacga tgccgcgctc atcgttattc
    10201 tgttcgatgg ccgccgccac gacgcgcgtc acaccgatgc catagcatcc catgaacatg
    10261 gtcacggact tgccctgttc gtcgagcacc gtggcattca tggcctcgct gtacttgcgg
    10321 ccgagctgga agatgtggcc gacctcgatg ccgcgcgcga tcccgagccg gccgcgaccc
    10381 gatgggctcg gatcccccgg cacgacgttg cggatgtccg ccgcctcggc ctcggggagg
    10441 tcgcggcccc agttgactcc cgtgaggtga tggtcgcgct cgttcgcgcc gcacacgaaa
    10501 tcggccgcgt tgagcgccgc gtgatcggcg tagaccttga gcttcaatcc caccggtccg
    10561 atgaaaccgg cctcgcagcc cgcggcctcg cgcacctgct ccgccgtggc catcttgagc
    10621 ggcgagaata ccccgggcag cttctgcgcc ttcaccgcgt tcaattcgtg gtcgcccctc
    10681 acgatgagcg cgaccacgcc accgtcgctg ccttcgacca gcagggtctt caggcagcgc
    10741 gaggcttcga tgccgaggaa cgccgtcagc tccgcgatgg tccgcacgtt cggggtggct
    10801 accctcgaca gcacggcgcc cggcgcaggg cgcccgcccg ccggtggcag cgcctcggcc
    10861 atctcgaggt tcgccgcgta gtcgtcgccg tcggaaaatg cgatggcgtc ttccccggac
    10921 tcggcgagca cgtggaattc ctgcgatgcg ctgccgccga tgctgccggt gtcggc
//
LOCUS       DAOMFD010000059        10607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1731_length_10607_cov_32.174090, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000059 DAOMFD010000000
VERSION     DAOMFD010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10607
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1731_length_10607_cov_32.174090"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..220)
                     /locus_tag="PL152_05200"
     CDS             complement(<1..220)
                     /locus_tag="PL152_05200"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the transfer of electrons from NADH to
                     quinone; Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HQR48710.1"
                     /translation="MGEIRSYTMNFGPQHPAAHGVLRLVLEMDGEVVQKADPHVGLLH
                     RGTEKLAESKPFNQSIGYMDRLDYVSMMC"
     gene            complement(213..929)
                     /locus_tag="PL152_05205"
     CDS             complement(213..929)
                     /locus_tag="PL152_05205"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HQR48711.1"
                     /translation="MTETTEQLATRVAERFAQKVTQVPSRCGEVTFEVEASSLLEVCR
                     ALRDEPEFGFEQLIDLAGVDYLAWAGGEWVTESATSTGFSRGREPVVGEAPAPGLSPE
                     DGAAKRFAVVYHLLSVTHNRRLRLRAWCDAGEPPMVDSVVDVWSSANWFEREAFDLYG
                     ILFRGHPDLRRILTDYGFVGHPFRKDFPLIGNVEVRYDPARARVVYEPVAIEPRTLVP
                     RVIREDNRYAEALKDAPRHG"
     gene            complement(936..1418)
                     /locus_tag="PL152_05210"
     CDS             complement(936..1418)
                     /locus_tag="PL152_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005914280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HQR48712.1"
                     /translation="MGLEPSGLPDRGFVTTTVDAVMNWARTGSMWPVTFGLACCAVEM
                     MHAGAARYDLDRFGIIFRPSPRQSDVMIVAGTLVNKMAPALRKVYDQMPEPRWVVSMG
                     SCANGGGYYHYSYAVVRGCDRVVPVDVYVPGCPPTAEALVYGLLQLQKKIHRTQTIAR
                     "
     gene            complement(1409..1765)
                     /gene="ndhC"
                     /locus_tag="PL152_05215"
     CDS             complement(1409..1765)
                     /gene="ndhC"
                     /locus_tag="PL152_05215"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003467430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HQR48713.1"
                     /translation="MLAAYLPILIFLAVAGGLGVLLLALGFAFGPRKPDPQKLSPYEC
                     GFEAFEDSRMKFDVRYYLVAILFIVFDLEIAFLFPWAVALDSVGVVGLAAMGVFLLVL
                     VVGFIYEWKKGALEWD"
     gene            complement(1883..1967)
                     /locus_tag="PL152_05220"
     tRNA            complement(1883..1967)
                     /locus_tag="PL152_05220"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1931..1933),aa:Leu,seq:gag)
     gene            complement(2111..2443)
                     /gene="secG"
                     /locus_tag="PL152_05225"
                     /pseudo
     CDS             complement(2111..2443)
                     /gene="secG"
                     /locus_tag="PL152_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918369.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
     gene            complement(2464..3216)
                     /gene="tpiA"
                     /locus_tag="PL152_05230"
     CDS             complement(2464..3216)
                     /gene="tpiA"
                     /locus_tag="PL152_05230"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014029893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HQR48714.1"
                     /translation="MRQPLVAGNWKMHGSRAENAALVRSLLDLQKAESRTEVMVCPPF
                     VYLWELGRLLKDTDVALGAQSVCAESQGAFTGEVSASMLKDVGCRYVLVGHSERRQIY
                     GEDDALVARKFVAAQSHGLVPVLCVGETLEEREGDRTAQVVARQLDAVLSVTGVAALD
                     RAVIAYEPVWAIGTGRTASPEQAQEVHGMIRGKVAALDGTIAASVRVLYGGSVKASNA
                     RELFAMPDIDGGLVGGASLKADEFARICAAAD"
     gene            complement(3330..4661)
                     /gene="glmM"
                     /locus_tag="PL152_05235"
     CDS             complement(3330..4661)
                     /gene="glmM"
                     /locus_tag="PL152_05235"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF008139.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HQR48715.1"
                     /translation="MGRRYFGTDGVRGRVGHDPMTVDFALRLASAAARVLAPEGGSAL
                     VGKDTRLSGYMFEAALEAGFVAAGVNVDLIGPLPTPAIAYLTQRFHRSFGVVISASHN
                     PYEDNGIKFFDASGGKLSDELEERIEACMQEPPLTRESMALGKAARVDRSRVVYQEFC
                     ASTLPKGMDLAGLKIVIDCANGAAYKVAPRVLADLGAEIIPIGCSPNGRNINLRCGST
                     APELLQLTVAGVGADVGIALDGDGDRLVMVDHLGRLVDGDQLMYVIARDRRAQGELTG
                     PVVGTVMSNLGLELALKEQGIEFLRAPVGDRYVLAMLRDKGGVLGGETSGHLLCLDRT
                     TTGDGLVSALQVLAVMKRTGRPLAELASGMQRFPQVMVNVRVKERVDPSQSPAIQAAV
                     RRVESALGGSGRVVLRASGTEPLIRVMVEGREEAVVTRYANDLAGVVKQAV"
     gene            complement(4664..5506)
                     /gene="folP"
                     /locus_tag="PL152_05240"
     CDS             complement(4664..5506)
                     /gene="folP"
                     /locus_tag="PL152_05240"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HQR48716.1"
                     /translation="MKLVLPDRVLPLDEPVVMGVLNVTPDSFSDGGIHAGVEGAVAHG
                     EKMVEEGAAIIDVGGESTRPGAAPVSADEEIRRVVPVIERLAGRVRVPISVDTSKPAV
                     MQAAIEAGAVLVNDVRALKMPGALEAVAAGRAAVCLMHMQGEPRSMQEAPRYDDVVAE
                     VREFLRARTAACERAGIPHERICVDPGIGFGKTPAHNLALLARLAELSGDGPPVLVGV
                     SRKSLVGIITGRPPGERLAGSIAFAALAVANGAAIIRAHDVAATVDAVRVACALRRAT
                     TGDA"
     gene            complement(5567..7483)
                     /gene="ftsH"
                     /locus_tag="PL152_05245"
     CDS             complement(5567..7483)
                     /gene="ftsH"
                     /locus_tag="PL152_05245"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HQR48717.1"
                     /translation="MNDLAKNIVLWIVIAVVLATVVSNFSSRSRDVQEMPYSTFLTHV
                     KDGTVAQVTFSGDRISGVRTGGERFVVYNPETDNSALIGTLQDHGVTFSAAPPERQSF
                     LMQLFISSFPILLLIGVWVYFMRQMQGGAGGRGAMSFGKSRARLLNEDQVTVTFADVA
                     GVDEAKEEVGEIVDFLKDPAKFQKLGGKIPRGVLMVGSPGTGKTLLAKAIAGEAKVPF
                     FTISGSDFVEMFVGVGASRVRDMFEQAKKHAPCIIFIDEIDAVGRHRGAGLGGGHDER
                     EQTLNQLLVEMDGFEGSEGVIVIAATNRPDVLDPALLRPGRFDRQVVVPLPDVRGREQ
                     ILKVHMRKLPLAEDVKPSVIARGTPGFSGADFANLVNEAALFAARANRRTVTMDEFER
                     AKDKIMMGAERKSMVMDEKEKKLTAYHEAGHAIVGLNVPDHDPVYKVTIIPRGRALGV
                     TMFLPEADRYSTSKRQLESRIATLFGGRVAEELIFGSDSVTTGASNDIERATEIARNM
                     VTKWGLSDKLGPLTYTEEDAEVFLGRSVTQHKQVSDVTAHAIDEEVRRVIDGNYRRAR
                     EILERDLEKLHRMADALIKYETIDESQIRDIMAGREPQPPDGWEDRDTPSAGAKPRER
                     RDGEAPTGRPAPQH"
     gene            complement(7550..8176)
                     /gene="rlmE"
                     /locus_tag="PL152_05250"
     CDS             complement(7550..8176)
                     /gene="rlmE"
                     /locus_tag="PL152_05250"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016436 - rRNA (uridine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HQR48718.1"
                     /translation="MPKRTKSSARWLAEHAADPYVKRAHDEGWRSRAAFKLEEIQRTD
                     RLLRPGMVVVDLGAAPGGWSQFAARILAGKGRVIALDVLEMPAIPGVEFIQGDFTDEA
                     VLGRLQGTLGGQKVDLVMSDMAPNMMGIADVDHDRSMQLVDLAVDFAARELRPGGDLL
                     MKVFQGREFQPLIARLRREFESVKLRKPKASRARSAEVYVLARGYRIV"
     assembly_gap    8418..8517
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8518..8720
                     /locus_tag="PL152_05255"
     CDS             <8518..8720
                     /locus_tag="PL152_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YhbY family RNA-binding protein"
                     /protein_id="HQR48719.1"
                     /translation="LVAELDRALHDHELVKVRARVGDRSERDAILGELARATRSELVQ
                     RIGHVALYYRRNPEKPGILLPD"
     gene            complement(8792..9268)
                     /gene="greA"
                     /locus_tag="PL152_05260"
     CDS             complement(8792..9268)
                     /gene="greA"
                     /locus_tag="PL152_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005106333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HQR48720.1"
                     /translation="MTRKPLTLKGAEKLRVELRRLKTEDRPRVIQAIAEARAHGDLSE
                     NAEYHAAREQQGFIEGRIKHIEHDLSHCQIIDVSHLPQGGKVVFGATVHLEDQENGSE
                     VVFQIVGEPEADVRHGMISITSPIARALVGKNVGDVVDVAVPGGTRAFEIVTVRYV"
     gene            complement(9265..>10607)
                     /gene="carB"
                     /locus_tag="PL152_05265"
     CDS             complement(9265..>10607)
                     /gene="carB"
                     /locus_tag="PL152_05265"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HQR48721.1"
                     /translation="ILHVEQPFGVIVQYGGQTPLKLAKPLDAAGVPIIGTSPDSIDIA
                     EDRERFQKLVDRLQLRQPPNRTARTEEEAILHARDVGFPLVVRPSYVLGGRAMEVVFS
                     EDDLRGYMTTAVQVSNDSPVLLDRFLDLAIEVDVDAVCDGKNVYIGGIMEHVEQAGVH
                     SGDSGCSLPPNSLSPELQEEIRDQTRRMALALNVIGLMNVQFAIQNGTVYVLEVNPRA
                     SRTAPFVSKATGVPLAKVGARVMTGRTLAELDLTRERVPEYYSVKEAVFPFGKFPDSD
                     PILGPEMKSTGEVMGTGRSFGEAYAKAQTASGVTIPTRGVCLISVRERDKPGAITLAR
                     ALIERGFEIVATRGTAAALEQAGVRCRSANKVREGRPHIVDMIKNDEIHLIVNTTEGK
                     RAVHESNSIRREAVARRVTYYTTLSGALATCEAIDHLQEVEVNRLQDLHKEVRV"
BASE COUNT         1624 a         3727 c         3486 g         1670 t
ORIGIN      
        1 tgcacatcat cgagacgtag tcgagccggt ccatgtagcc gatcgactgg ttgaagggct
       61 tcgattcggc gagtttctcg gtgccccggt gcagcaggcc gacgtgcggg tcggctttct
      121 gcacgacctc gccgtccatc tcgagcacga gccgcagcac gccgtgcgcc gcggggtgct
      181 gcggaccgaa gttcatggtg tagctgcgga tctcacccat ggcgcggagc gtccttcagc
      241 gcctcggcgt agcggttgtc ctcgcggatc acgcgcggca ccagcgtgcg cggctcgatc
      301 gccacgggct cgtacaccac gcgggcccgt gcggggtcgt agcgcacctc gacgttgccg
      361 atgagcggga agtccttgcg gaacgggtgc ccgacgaagc cgtagtcggt gaggatgcgg
      421 cgcaggtccg ggtggccacg gaacaggatg ccgtagagat cgaaggcttc ccgctcgaac
      481 cagttggccg acgaccacac gtcgacgacg gaatccacca tcggcggctc gccggcgtcg
      541 caccaggcgc gcagccgcag gcggcggttg tgcgtgaccg acagcaggtg gtagacgacg
      601 gcgaagcgct tcgcggcgcc atcctccggg gacagtccgg gcgcaggtgc ctcgccgacc
      661 accggctccc ggccgcggct gaaaccggtc gacgtcgccg attccgtgac ccactcgccg
      721 ccagcccagg ccaggtagtc cacgccggcc aggtcgatca actgctcgaa cccgaattcc
      781 ggctcgtcac gcagcgcgcg acagacctcg aggaggctcg aggcttccac ctcgaacgtg
      841 acctcgccgc agcgcgaagg cacttgcgtg accttctgcg cgaatcgctc ggccacgcgc
      901 gtggccagtt gttccgttgt ctcggtcatg atgggtcagc gggcgatggt ctgcgtgcgg
      961 tggatcttct tctgcaactg caggagcccg tacaccaggg cttcggccgt gggcgggcat
     1021 cccggcacgt acacgtccac cgggacgacc cggtcgcagc cgcgcaccac ggcgtaggag
     1081 tagtggtagt aaccaccgcc gttcgcgcag gaacccatgg agaccaccca gcgcggctcc
     1141 ggcatctggt cgtacacctt gcgcagcgcc ggggccatct tgttcaccag cgtgccggcc
     1201 acgatcatca cgtcggactg gcgcgggctg gggcggaaga tgatgccgaa gcgatcgagg
     1261 tcgtagcgcg ccgcacccgc gtgcatcatc tccacggcac agcaggccag gccgaaggtc
     1321 accggccaca tcgagccggt gcgggcccag ttcatcaccg cgtccacggt cgtggtgacg
     1381 aagccgcgat cgggcagccc ggagggctct agtcccattc cagggctccc ttcttccatt
     1441 cgtagatgaa gcccaccacg agcaccagca ggaatacacc catcgcggcg agaccgacca
     1501 cgcccaccga atcgagcgcg accgcccacg gaaacaggaa tgcgatctcg aggtcgaaga
     1561 cgatgaacag gatcgcgacg aggtagtagc gcacgtcgaa cttcatgcgc gaatcctcga
     1621 acgcctcgaa cccgcactcg tacggggaga gcttctgggg atcgggcttg cgcggcccga
     1681 atgcgaatcc gagcgccagg agcagcacgc cgaggccgcc cgcgacggcc agaaagatca
     1741 ggatcggcag gtacgctgca agcatcgcgc ggaggctccg ggtcgaggcc cgacgagccc
     1801 ggaattttag cagggtccgc tgcagcgccg taaggtcgcg ggaatgaaaa aggcgagccc
     1861 gtggctcgcc ttgctgtcgt catggtgcgg atggagagac tcgaactctc acgccttacg
     1921 gcactacccc ctcaagatag cgtgtctacc aattccacca catccgcacg cgcttgcctg
     1981 gtcgccccta ctggccggac ggtgccggtg ccggctgctg cgccggcacc tgctgagtct
     2041 gcgcgcccgt tgcatccggc gccggcgcag tcgtcggcga tgccggcgca ggtgcgctcg
     2101 tgctcgcggg aggcaacgtg gccggtggct gactcgccgg cacgccggtc gcgggctggg
     2161 tctgttcggt cgccagccga tccatgacgc tggtgggcgc cgtcccgtgg ctgaacaggt
     2221 acgacagcac gaggctcgag acgaagaaca gggtcgcgag caccgccgtg gtgcgcgaca
     2281 ggaacgaggc cgagccgcgc gaaccgaaca ccgtgcccga cgcaccggcg ccgaatcccg
     2341 cgcccgcgtc cgcgccacgg ccgcgctgca gcagcaccag tcctacgatc gacaccgcca
     2401 gcagcacgtg tagcacgacc gccagcgtct gtaaccagct catttccgct ccatatccga
     2461 atgtcagtcc gcggccgcgc agatccgcgc gaattcgtcc gccttgagcg acgcgccgcc
     2521 gaccaggccg ccgtcgatgt ccggcatcgc aaagagttcc cgcgcgttcg acgccttgac
     2581 gctgccgccg tacaggaccc tcacggaggc ggcgattgta ccatccagcg ccgccacctt
     2641 gccgcggatc atgccgtgca cttcctgggc ctgctccggc gacgccgtcc gtcccgtgcc
     2701 gatggcccag accggttcgt aggcgatgac cgcccggtcg agcgccgcga cgcccgtcac
     2761 ggacagcacg gcatcgagtt gccgcgcaac cacctgcgcc gtgcggtcgc cctcgcgttc
     2821 ctcgagcgtc tctccgacgc agagcaccgg caccagcccg tgcgactgcg ccgccacgaa
     2881 cttccgcgcc acgagcgcgt cgtcctcgcc gtagatctgg cggcgctcgg agtggccgac
     2941 cagtacgtag cgacagccca cgtccttgag catggacgcg gacacttcgc cggtgaacgc
     3001 gccttgcgat tccgcgcaca ccgactgcgc gccgagcgcg acgtccgtgt ccttgagcag
     3061 ccgcccgagc tcccacaggt acacgaaggg cgggcagacc atgacctcgg ttcgcgattc
     3121 cgccttctgc aggtcgagca gcgagcggac cagcgccgcg ttctctgccc tggaaccgtg
     3181 catcttccag ttacccgcga ccagcggctg acgcatggga gactccaccc tgagtgcagg
     3241 cggggtccgc tcgcctgagg agcggctgcc cgcgaaaggc gcgcgatctt agcggcgggt
     3301 ccgcaggaat gcaattcgca ggggccgcgt cacacggcct gcttcacgac cccggcgagg
     3361 tcgttcgcgt agcgcgtgac gacggcctct tcccggccct ccaccatcac ccggatcagc
     3421 ggttccgttc ccgacgcgcg cagcacgaca cgacccgagc caccgagggc cgattccacg
     3481 cgcctgacgg cggcctggat cgccggggac tggctcgggt ccacccgttc cttgaccctg
     3541 acgttgacca tcacctgcgg gaagcgctgc atgccggaag ccagctcggc cagcggccgt
     3601 ccggtgcgct tcatgacggc cagcacctgc agcgcgctca cgaggccgtc gcccgtggtc
     3661 gtccggtcga ggcacaacag gtgacccgat gtctcgccgc cgagcacacc gcctttgtcg
     3721 cgcagcatcg ccagcacgta gcgatcgccc accggcgccc gcaggaactc gatgccctgc
     3781 tccttgagcg caagttccag gccgaggttg ctcatcaccg tcccgaccac gggcccggtg
     3841 agttcgccct gcgcgcggcg atcgcgcgcg atcacgtaca tcagctggtc gccgtccacg
     3901 agccgcccca ggtgatcgac catcaccagc cggtcgccgt cgccgtcgag cgcgatgccc
     3961 acgtcggcgc cgaccccggc gaccgtgagc tgcagcagct cgggcgccgt cgatccgcag
     4021 cgcaggttga tgttgcggcc attcggcgag cagccgatcg ggatgatctc ggccccgagg
     4081 tccgcgagca cgcgcggcgc caccttgtag gcagccccgt tggcgcagtc gataacgatc
     4141 ttgaggccgg ccaggtccat gcccttcggc agcgtcgagg cgcagaactc ctggtagacg
     4201 acgcgcgagc gatccacgcg tgcggccttg ccgagggcca tcgactcccg cgtcagcggc
     4261 ggctcctgca tgcaggcctc gatgcgctcc tcgagttcgt ccgacagctt gccgcccgac
     4321 gcgtcgaaga acttgatgcc gttgtcttcg tacggattgt gcgaggccga aatgaccacg
     4381 ccgaagctgc gatggaagcg ctgggtcagg taggcgatgg ccggggtcgg cagcggaccg
     4441 atgaggtcca cgttgacgcc cgccgcgacg aacccggcct cgagagccgc ctcgaacatg
     4501 tagcccgaca ggcgcgtgtc cttgccgacg agcgcgctgc ccccttccgg ggcgaggacc
     4561 cgcgccgcag cgctggccag cctcagcgcg aaatccaccg tcatcgggtc gtggccgacc
     4621 cggccgcgca cgccgtccgt gccgaagtac cgtctgccca tcgtcacgcg tctcctgtcg
     4681 tcgcgcgcct gagcgcacac gcaacccgga ccgcatcgac cgtcgccgcg acgtcgtggg
     4741 cccggatgat ggccgccccg ttcgccaccg ccagggcggc gaaggcgatg ctgccggcca
     4801 gccgttcgcc gggcggtcgg ccggtgatga taccgaccag cgacttgcgc gacacgccga
     4861 ccagcaccgg cggcccgtcg cccgagagct ccgcaagccg cgcgagcagc gcaaggttgt
     4921 gcgccggagt cttgccgaag ccgatgcccg ggtccacgca gatccgctcg tgcgggatgc
     4981 cggcacgctc gcaggccgcg gtccgcgccc gcaggaactc gcgcacctcg gcgacgacgt
     5041 cgtcgtagcg cggcgcctcc tgcatggatc gaggttcccc ctgcatgtgc atcaggcaca
     5101 cggccgcacg cccggcggcg acggcctcga gcgcgcccgg catcttgagc gcgcgcacgt
     5161 cgttgaccag gaccgcgccg gcctcgatgg cggcttgcat gaccgcgggc ttgctcgtgt
     5221 ccaccgatat cggcacgcga acccttccgg cgagacgctc gatcacgggc acgacgcggc
     5281 ggatctcctc gtccgcactg accggcgcgg caccgggccg cgtcgattcg ccaccgacgt
     5341 cgatgatcgc ggcgccctcc tcgaccatct tctcgccgtg ggccacggcg ccctccacgc
     5401 cggcgtgaat cccgccgtcg gagaaggaat cgggagtcac gttcaggacg cccatgacca
     5461 cgggctcgtc gaggggcagg acccggtccg ggagcaccag tttcatcggg atttcgcggc
     5521 gccgcaaacg aagcgcccgg cgtgggccgg gcgctcacgc gtcctgtcag tgctgcggtg
     5581 cgggccggcc ggtcggcgcc tcgccatcgc gccgttcccg gggcttcgcc ccggcgctcg
     5641 gcgtgtcgcg atcctcccag ccgtcgggcg gctgcggctc gcggcccgcc atgatgtcgc
     5701 ggatctgcga ctcgtcgatg gtctcgtact tgatcagcgc gtccgccatg cggtgcagct
     5761 tctcgaggtc gcgctcgagg atctcacggg ccctgcggta attgccgtcg atgacgcgcc
     5821 gcacttcctc gtcgatcgcg tgcgccgtga cgtccgacac ctgcttgtgc tgggtgaccg
     5881 agcggccgag gaacacttcg gcgtcctctt cggtgtaggt cagcgggccg agcttgtccg
     5941 acaggcccca cttggtgacc atgttgcggg cgatctcggt cgcccgctcg atgtcgttcg
     6001 acgctccggt cgtgaccgaa tcggaaccga agatcagctc ctccgccacg cgaccgccga
     6061 acagcgtcgc gatgcggctt tcgagctggc gcttgctcgt gctgtagcga tccgcctccg
     6121 gcaggaacat cgtgacgccg agcgcacggc cgcgcgggat gatcgtcacc ttgtagaccg
     6181 ggtcgtggtc ggggacgttc aggcccacga tcgcgtggcc ggcctcgtgg taggcggtga
     6241 gcttcttctc cttctcgtcc atcaccatgg acttgcgctc ggcgcccatc atgatcttgt
     6301 ccttggcgcg ctcgaactcg tccatcgtca cggtgcgacg attcgcccgc gcggcgaaca
     6361 gcgccgcctc gttgacgagg ttggcgaagt ctgcgcctga gaaccccggc gtgccgcgcg
     6421 cgatgacgga aggcttcacg tcctcggcca gcgggagctt gcgcatgtgg accttcagga
     6481 tctgctcgcg gccccgcacg tcgggcagcg gcacgaccac ctggcggtcg aaccgtcccg
     6541 ggcgcagcag cgccgggtcg agcacgtccg ggcggttggt ggccgcgatg acgatcacgc
     6601 cctcgctgcc ctcgaagccg tccatctcga cgagcagctg gttcagcgtc tgctcgcgct
     6661 cgtcgtggcc gccgccgagg ccggcgccgc gatgacggcc gaccgcgtcg atctcgtcga
     6721 tgaagatgat gcacggcgcg tgcttcttcg cctgctcgaa catgtcgcgc acgcgcgagg
     6781 cgccgacgcc gacgaacatc tcgacgaagt cagagcccga gatcgtgaag aagggtacct
     6841 tggcctcgcc ggcgatcgcc ttggcgagca gggtcttgcc cgtgccgggc gagcccacca
     6901 tcagcacgcc gcgcgggatc ttgccgccga gcttctggaa cttggccgga tccttgagga
     6961 aatcgacgat ctcgccgact tcttccttgg cctcgtcgac gccggcgacg tcggcgaagg
     7021 tgacggtcac ctggtcttca ttcaggagcc gcgcgcggct cttgccgaac gacatggccc
     7081 cccgccctcc ggccccgccc tgcatctggc gcatgaagta cacccagacg ccgatcagca
     7141 gcaggatcgg gaacgacgag atgaacagct gcatcaggaa cgactggcgc tccgggggcg
     7201 cggcgctgaa ggtcacgccg tggtcctgca gcgtgccgat gagcgcggaa ttgtccgttt
     7261 ccgggttgta gaccacgaag cgctcgccgc cggtgcgcac gccgctgatg cggtcgccgc
     7321 tgaaggtgac ctgggcgacg gtcccgtcct tcacgtgggt gaggaacgtg gagtacggca
     7381 tctcctgcac gtccctggaa cgcgagctga aattgctcac gacggtcgcc agcacgaccg
     7441 cgatgacgat ccacaggacg atgttttttg ccagatcgtt caatgccttg acctcgagcc
     7501 agccggcgcg gatgcgggct gggaatggcg acggggcgcc cggatgacac tacacgattc
     7561 gatacccgcg tgccagaacg tagacctcgg cgctgcgggc ccgggacgcc ttcggcttgc
     7621 gcagcttcac ggattcgaat tcccgccgca ggcgcgcgat cagcggctgg aactcccggc
     7681 cctggaacac cttcatcagc aggtcaccac ccgggcgcaa ctcgcgggcc gcgaaatcga
     7741 ccgcgaggtc cacgagttgc atcgagcggt cgtggtccac gtccgcgatg cccatcatgt
     7801 tgggggccat gtcggacatt acaagatcga ccttctggcc gccgagcgtc ccttggagcc
     7861 ggcccagcac cgcctcgtcc gtgaagtcgc cctggatgaa ctccaccccc ggaatcgccg
     7921 gcatctcgag gacatcgagc gcgatcacgc gccccttgcc ggccagtatc ctggccgcga
     7981 actggctcca gccccccggc gccgccccca ggtcgaccac gaccatcccg ggacgcagga
     8041 gccggtcggt gcgctggatc tcctcgagct tgaacgccgc cctggagcgc cagccctcgt
     8101 cgtgcgcgcg cttcacataa ggatcggcgg cgtgctcggc gagccagcgg gcggaactct
     8161 tggtgcgctt gggcatcggg ttgcggattg cgatcaaatc aatggttgcc gcaggggatg
     8221 gccgagagtc cggcgcgctc gccgaagtcc tcatacacta tcctcctgat gtcactcacc
     8281 gaagcccagc gcaagcacct gcgacgactt ggacacgatc gcaatccgat cgttctggtc
     8341 ggtcaggccg gcatcgggcc gaacctggtc gcggaactgg atcgggccct gcacgaccac
     8401 gagctcgtca aggtgcgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnacc
     8521 tggtcgcgga actggatcgg gccctgcacg accacgagct cgtcaaggtg cgtgcccgcg
     8581 tcggggaccg cagcgagcgc gacgccattc ttggcgagct ggcgcgcgcg acgcgctcgg
     8641 agctcgtgca gaggatcggc cacgtggcgc tgtattaccg ccgcaatccg gaaaagccgg
     8701 gaatcctgct ccccgactga ggctgcggcc accgaagaaa ttcggaattc ggtgccaggc
     8761 accgaatttc cgcgcctgcg ccgtccggga ctcagacgta gcggaccgtc acgatctcga
     8821 acgcccgggt gccccctggc acggccacgt cgacgacgtc acccacgttc ttgccgacca
     8881 gtgcccgcgc gatcggcgag gtgatcgaga tcatcccgtg ccggacgtcg gcctcgggct
     8941 cgccgacgat ctggaacacg acctccgatc cgttctcctg gtcctcgagg tgcacggtcg
     9001 ctccgaacac gaccttgcct ccctgcggca ggtgcgagac gtcgatgatc tggcagtggg
     9061 acaggtcgtg ctcgatgtgc ttgatgcggc cttcgatgaa tccctgctgc tcgcgcgcgg
     9121 cgtgatattc cgcgttctcg ctcaagtcgc catgggcccg cgcctccgcg atcgcctgga
     9181 tcactcgcgg ccggtcctcg gtcttgagcc ggcgcagttc cacacggagc ttctcagcac
     9241 ccttcaatgt cagaggctta cgagtcatac ccgaacctct ttgtgaagat cctggaggcg
     9301 gttgacctcg acctcctgga ggtggtcgat ggcttcgcag gtcgccagcg ccccggagag
     9361 cgtcgtgtag tacgtgaccc ggcgcgcgac ggcctcgcgg cggatcgagt tcgactcgtg
     9421 cacggcccgc ttgccttcgg tcgtgttgac gatcaggtga atctcgtcgt tcttgatcat
     9481 gtcgacgatg tgcggccgcc cttcgcggac cttgttggcg gaccggcagc gcacccccgc
     9541 ctgctcgagc gccgcggcgg tgccacgcgt ggcgacgatc tcgaagccgc gctcgatgag
     9601 cgcgcgtgcg agggtgattg cgcccggctt gtcgcgctcg cgcacgctga tcaggcacac
     9661 gccgcgggtc gggatggtca cgcccgacgc cgtctgcgcc ttcgcgtagg cctcgccgaa
     9721 gctgcgtccc gtgcccatca cctcgcccgt ggacttcatc tcgggcccga ggatcgggtc
     9781 ggagtccggg aattttccga aggggaacac cgcctccttc accgagtagt actcgggcac
     9841 gcgctcgcgc gtgaggtcga gttcggcgag cgtcctgccc gtcatcacgc gggcgccgac
     9901 cttggcgagc ggcacgccgg tcgccttcga gacgaacggc gccgtgcgtg aggcccgcgg
     9961 attcacttcc aggacgtaca cggtcccgtt ctggatggcg aactgcacgt tcatgagccc
    10021 gatcacgttc agcgccagcg ccatgcgccg cgtctggtcg cggatctcct cctggagctc
    10081 cgggctcagg ctgttcgggg gcagcgagca gccggagtcg cccgagtgca cgccggcctg
    10141 ctcgacgtgc tccatgatgc cgccgatgta gacgttcttg ccgtcgcaga ccgcgtccac
    10201 gtcgacctcg atggcgaggt cgaggaagcg gtcgagcagc actgggctgt cgttggacac
    10261 ctgcaccgcg gtcgtcatgt atccgcgcag gtcgtcctcg gagaacacca cttccatggc
    10321 gcgaccgccg agcacgtagg agggccgcac gaccagcggg aagccgacgt cgcgcgcatg
    10381 caggatcgcc tcttcttccg tgcgggccgt gcgattgggc ggctggcgca gctgcagccg
    10441 gtccaccagc ttctggaagc gctcgcggtc ctccgcgatg tcgatcgagt ccgggctcgt
    10501 gccgatgatc ggcacgccgg ccgcgtcgag cggcttggcc agcttgagcg gcgtctggcc
    10561 gccgtactgc acgatcacgc cgaacggctg ctcgacgtgc aggatct
//
LOCUS       DAOMFD010000060        10606 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1732_length_10606_cov_34.296654, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000060 DAOMFD010000000
VERSION     DAOMFD010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10606)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1732_length_10606_cov_34.296654"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..330
                     /locus_tag="PL152_05270"
     CDS             <1..330
                     /locus_tag="PL152_05270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48722.1"
                     /translation="IRLDIENRREEIEVTKLVGGSDAFVRRPFLYNGVWYGLGGGAVA
                     WSIVAVVILVLAEPVRRIAGLYGSGYALHGLDATASLALVLGGVLLGWLGSFIVSTRE
                     LRGIEPR"
     gene            509..1384
                     /gene="rpoH"
                     /locus_tag="PL152_05275"
     CDS             509..1384
                     /gene="rpoH"
                     /locus_tag="PL152_05275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HQR48723.1"
                     /translation="MTANALVRRSPAELALSGPIGSLDAYFDAIGRIPVLSREEETAL
                     AIRYRSENDLEAARQLVLSHLRFVVHIARGYLGYGLPMGDLVQEGNVGLMKAVKRFDP
                     TVGVRLVSFAVHWIRAEIHEYVLRNWRLVKVATTKAQRKLFFNLRKYKRNLGWLTTEE
                     TATVARELGVSTSEVSEMERRLASRDLSYDPAPDADEEEESWSPAAYLPAPDADPALA
                     VEHAEWDDDVSDKVAAAMSQLDERSQAILRARWMSESKATLHDLADQYGVSAERIRQI
                     EANAIKKLRKLVAEE"
     gene            complement(1472..2161)
                     /locus_tag="PL152_05280"
     CDS             complement(1472..2161)
                     /locus_tag="PL152_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HQR48724.1"
                     /translation="MAMRSKAALLSVVAVTVVLSGCESMQDFASNPEKQKTRQGTAIG
                     AVGGAAVGLLVGGGWKGALIGAGVGAIAGGLVGNYQDKQEAKLRQQMAGTGVEVVRMG
                     DNLTLSMPGNVTFAFDSAKLNPQFDPVLDKLAQTLTEYNQTVIQIAGHTDSIGTKAYN
                     MNLSTQRADSVKAYLVGRGVPAARLVTIGAGPDYPIASNDTEQGRAENRRVEITIVPV
                     TQDTLNQAKQG"
     gene            2457..3662
                     /locus_tag="PL152_05285"
     CDS             2457..3662
                     /locus_tag="PL152_05285"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48725.1"
                     /translation="MATPLSAPREKKSSRALNPMDLFDVRGLLSEEEQMVQDSVARLV
                     DERVLPIIREHFENHTFPRELVGELAGLGLLGSSLEGYGCAGMNAVSYGLICQELERG
                     DSGLRSFVSVQSSLCMYPIHAYGSEEQKQKYLPRMARGEILGCFGLTEPHGGSDPANM
                     KTHAKRRGRDWVLNGAKMWITNSPIADLFVIWAKTEEGIRGFLVEKGTKGLTAPEIEK
                     KFSLRASITGAIFLEDVVVPEENMLPGSTVGIKAPLSCLTQARYGITWGVIGAAQACL
                     AQVLDYSQTRILFGRPLAATQTIQIRLAEMARRITTAQLLSLQLGRLKDAGTMSPSHV
                     SLAKWNNCRIALDVARDARDILGGAGISAEYVPIRHALNLESVITYEGTETIHQLTIG
                     RELTGINAF"
     gene            complement(4048..5862)
                     /locus_tag="PL152_05290"
     CDS             complement(4048..5862)
                     /locus_tag="PL152_05290"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HQR48726.1"
                     /translation="MNGEQRGGVDITRRDLLKAIGAGAVATTLGAAAGSAAAATANDP
                     QPGAAPGPAGSPYNILFILTDQERLFRPGELPGDYRLPAHERLAQRGTFFENHHINSC
                     VCTPSRSVLYTGQHMQHTRMFDNTNFPWISSMSTDIPTVGDLLREAGYYTAYKGKWHL
                     TKEFETVNELGAPTKIFTREMEEYGFSDYVGVGDIIAHDQGGYLHDGIISAMGVSWLR
                     GKGAELATQGKPWFLAVNLVNPHDVMYLNTDRPGEPVQARNILSHIRPEPANTSYGRQ
                     WTFDLPATYGERLDAPGRPAAHTDYLKSHDGLTGHIPNEDWRWRRRHNYYLNCLRDVD
                     RQIAALLDELDALGLASRTIVILTADHGDLDGAHRLHAKGATAYREQNNVPLIVAHPA
                     YPGGRRCRAVTSHLDIAPTLLGLTDATPAKKAAVAKHLPGRDFSVLLAAPETASYTSA
                     RDGALFCYNMFAYIDGDFMQKVVAVMQQPDGKAKLQEAVKAGTLRPDLNKRGAIRSVF
                     DGRYQLTRYFSPRQHNRPASIEALFKLNDVELFDLERDPLQRDNLALDRDRSRELLET
                     MNAKLNALIDREVGEDVGQMLPANVSGGWVTTDAVMDV"
     assembly_gap    6006..6105
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6106..6349
                     /locus_tag="PL152_05295"
     CDS             <6106..6349
                     /locus_tag="PL152_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48727.1"
                     /translation="MASGFFSDVPDMKLVCDGVRCAGSHVIYLWTFTGHAARTWNVIS
                     VHGWEEWDVGGDLQVKASRGWYDSDDYARQVDGATA"
     gene            6631..7392
                     /locus_tag="PL152_05300"
     CDS             6631..7392
                     /locus_tag="PL152_05300"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HQR48728.1"
                     /translation="MTQGADLPQIIAHRGNAAEFPENTLQALASAVELGVKHLEFDVQ
                     LTSDRVPVVLHDSDLERVGDVSEVVHDMTWAQLSETPVGEFKRFGSRFAYTYPPSLAE
                     VVHALAGWEGVTAFVEIKRASLRRFGREAVLRQVAQVLKPVLDRCVVISFDLPSVQVM
                     RRMTGARIGWVISSYGDDTLAEATRTAPDFLFANLERLPQGLQALWPGPWQWAIYEVR
                     DIRTALDCQALGAAFVESMAVRGLGLAFEEYRRQW"
     gene            7389..8822
                     /locus_tag="PL152_05305"
     CDS             7389..8822
                     /locus_tag="PL152_05305"
                     /inference="COORDINATES: protein motif:HMM:NF012589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HQR48729.1"
                     /translation="MSAPTPGLILALDQGGHGSRAVLFDETGSELTEAHAPVATRHGE
                     NGWVEQDPLELLRSLHVAARDACDSPAATGRPVIAAGLATQRSTIVCWERSTGRALTD
                     AISWQDRRNAAWLEQLRPVEPEIRRLTGLVLSAHYGASKLRWCLDHVPGVQLAARRGD
                     LAFGPLASFLLARLLDERPSVADPANASRTLLYDPTRLDWSPPLLDTFGIDRSCLPAC
                     VPTRHAYGRMRVGARHIPLTACTGDQSAAALAFGAPSQSVALVNAGTGAFVQRAVPAG
                     AVLPDGLLCSVLRSDGVHATFSHEGTVNGAGSAVEWLGSRIALDVRRAIAVLDVGPSD
                     RVPLFMNGVGGLGSPYWKANFPVEFVGEGSDAMKLAAVVESIAFLLCANLETMRRSAP
                     LDGIRISGGLASSDYLCQSLADASRLPVDRHALREATARGVAFLAAGEPAGWHAPPLE
                     RAFVPRRNDPLAARHAAWLGEMARRGA"
     gene            complement(8823..9812)
                     /locus_tag="PL152_05310"
     CDS             complement(8823..9812)
                     /locus_tag="PL152_05310"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR48730.1"
                     /translation="MTVGEFAPPAWLANPHVQSVFPSLPFRRPAVAWHTRRLRAASEE
                     LLVDCGDGVRLLAHHATQERIGRAPAARVAVLLHGWEGSSDSLYILSLGQYLLDRGFD
                     VVRLNLRDHGGSHHLNPEIFHSCRIKEVVGAVRRIQDTNRGRGVNLVGFSLGGNFFLR
                     VAARARAAGIELERVIAVSPVVDPEHTLWRLELGWPVYRRYFVWKWRGSLRLKQSAWP
                     ELYDLSDVRAMKTLTEMTDHLACKYGGYPTLEAYLKGYAIVGEALDSIVHPTRIITAA
                     DDPIIPVEDFDRIARPAALTVTRTRFGGHCGYYDGRRGGTWIEREVYAALAGG"
     gene            complement(9809..>10606)
                     /locus_tag="PL152_05315"
     CDS             complement(9809..>10606)
                     /locus_tag="PL152_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48731.1"
                     /translation="RLGTEIREQAQKPATPTVVELPAPEPPPRQWWPVALTALLAVIP
                     ALVLGLLYWQELKVNRDYGAQISQLQDSVAKLARTLPLALAPAPVAVPADQPGDVTGA
                     PQPQALSSAAVPAPARPEYVPYGDIPFGGARLARLQSLVASLEAQRFRGRIVLEYSVG
                     DFCLAGSQAQGFEVADPSLPIRKCDIVGNPFDDSLSAAQRQSVDFANYVAMLARRSGG
                     AITVESVSAGRRHPVEYPEQTEATTAGTWNTVAAQNNRVDFRVVTAE"
BASE COUNT         1620 a         3547 c         3618 g         1721 t
ORIGIN      
        1 atccgcctcg acatcgagaa ccggcgcgag gagatcgagg tcacgaagct ggtcggcggc
       61 agcgatgcgt tcgtgcgccg gccgttcctc tataacggcg tctggtacgg gctcggcggc
      121 ggcgccgtgg cgtggtcaat cgtggcggtc gtcatcctcg tgctggccga accggtgcgg
      181 cgcatcgccg gcctgtacgg cagcggttat gccctgcatg ggctcgatgc caccgcgagc
      241 ctggcgctgg tgctgggcgg tgtcctgctc ggctggctcg gctcgttcat cgtctcgacg
      301 cgcgagctgc gcggcatcga gccgcggtag gcggccgccg tgccgcgggc ttcgggcctg
      361 cccggcggtc cgcttataac cgtttgtttc ttatcaggat atctggccat aagcgccggc
      421 ttggtcgctg aacttcccgg ctatttagca ctctaaaacc gagactgcta atatctttgg
      481 ccgtgactgg ggagagcccg aaaccaacat gaccgccaac gcacttgtcc gtcgtagtcc
      541 tgccgagctg gcactgtccg gcccgatcgg gagcctggac gcgtacttcg acgcgatcgg
      601 ccgcatcccg gtgctgagcc gcgaggagga gacggcgctc gccatccgtt atcgcagcga
      661 gaacgacctc gaggcggcgc gccagctggt gctgtcgcac ctgcggttcg tcgtgcacat
      721 cgcccgcggc tacctcggct acggcctgcc gatgggcgac ctcgtgcagg aaggcaacgt
      781 gggcctgatg aaggccgtga agcgcttcga cccgacggtc ggcgtgcgcc tcgtctcgtt
      841 cgccgtgcac tggatccgcg cggagatcca cgaatacgtg ctgcgcaact ggcgcctcgt
      901 gaaggtcgcc acgaccaagg cgcagcggaa gctcttcttc aacctgcgca agtacaagcg
      961 gaacctcggc tggctcacca ccgaggagac ggcgaccgtg gcccgcgagc tcggcgtcag
     1021 cacgagcgag gtctccgaga tggagcgccg gctcgcttcg cgcgacctct cttatgaccc
     1081 cgcacccgat gccgacgagg aagaggagtc gtggtctccg gccgcgtacc tgccggcgcc
     1141 cgatgccgac cccgcactcg cggtcgaaca cgcggagtgg gacgacgacg tgagcgacaa
     1201 ggtcgcagcc gcgatgtcgc agctcgatga gcgcagccag gcgatcctgc gcgcgcgctg
     1261 gatgagcgag tcgaaggcga cgctgcacga cctcgccgac cagtacggcg tgtcggcgga
     1321 gcgcatccgc cagatcgagg cgaacgcgat caagaagctg cgcaagctcg tcgccgaaga
     1381 ataggaatag gaaatagggg acattctcct atttccgccg tcggaaatag gagggaaata
     1441 ggagaatgtc ccctatttcc ctatttcctg atcagccctg cttggcctgg ttgagcgtgt
     1501 cctgcgtcac cgggacgatc gtgatctcga cgcggcggtt ctcggcacgg ccctgctcgg
     1561 tgtcgttcga cgcgatcggg taatccgggc ccgcgccgat cgtcaccagc cgcgccgccg
     1621 gcacgccgcg acccacgagg tacgccttca cgctgtcggc acgctgcgtc gacaggttca
     1681 tgttgtaggc cttggtgccg atgctgtcgg tgtggcccgc gatctggatc accgtctggt
     1741 tgtactcggt gagggtctgg gccagcttgt cgagcaccgg gtcgaactgc gggttcagct
     1801 tggcgctgtc gaaggcgaac gtgacgttgc ccggcatgct gagcgtcagg ttgtcgccca
     1861 tgcgcacgac ctcgacgccg gtcccggcca tctgctgacg cagctttgct tcctgcttgt
     1921 cctggtagtt accgacgagg ccgccggcga tcgcgcccac gccggccccg atcagcgcgc
     1981 ccttccagcc gccgcccacg agcagcccga ccgcggcacc gcccaccgca ccgatcgcgg
     2041 tgccctgccg ggtcttctgc ttctccgggt tgctcgcgaa gtcctgcatg gactcgcacc
     2101 cggaaagtac gaccgtgacc gcgaccaccg acaggagtgc tgctttgctg cgcattgcca
     2161 tggacatgct ccgaagctgg ccgggacgcg cgccccggca tctggttgag agggatctgc
     2221 cgtcatgatg tcgcgatgcg ggtcgggtgg caacggctcc gcgcggcgcg cagccggacg
     2281 cctgcaattc cgtgggtccc gcccggcctt tcggcaaatg tcgaatgtgc cccgggccgg
     2341 gaccacgtaa aatcgcgcgc catgtgcacg ccgacgggcg cccgggccat gcagggcgac
     2401 gggttcggcg gcatcgcttg ccgacaaacc acagcgcaaa cctgccggga ggccacatgg
     2461 ccacgccgct ttccgcaccc cgcgagaaga agtcctcgcg agccctgaac ccgatggacc
     2521 tcttcgacgt ccgtggcctg ctgagcgaag aggagcagat ggtgcaggac tccgtcgccc
     2581 gcctcgtcga cgagcgggtc ctgccgatca tccgcgagca cttcgagaac cacacgttcc
     2641 cgcgcgagct ggtgggggaa ctcgctggac tcggactgct cggcagctcg ctcgagggtt
     2701 acggctgcgc cggcatgaac gcggtgagtt atggcctgat ctgccaggaa ctcgagcgcg
     2761 gcgactcggg cctgcgcagc ttcgtgtccg tgcagtcgag cctctgcatg tacccgattc
     2821 acgcctacgg cagcgaggaa cagaagcaga agtacctgcc gcgcatggcg cgcggcgaga
     2881 tcctcggctg cttcgggctc accgagccgc acggcggctc ggaccccgcc aacatgaaga
     2941 cgcacgcgaa gcgccgcggc cgcgactggg tgctgaacgg cgcgaagatg tggatcacca
     3001 attcgccgat cgcggacctg ttcgtgatct gggcgaagac cgaggaaggc atccgcggct
     3061 tcctggtcga gaaaggaacg aagggtctca ccgctcccga aatcgaaaag aagttctcgc
     3121 tgcgcgcctc gatcaccggc gcgatcttcc tcgaggacgt cgtggtcccg gaggagaaca
     3181 tgctccccgg cagcaccgtc ggcatcaagg cgccgctctc gtgcctcacg caggcgcgct
     3241 acggcatcac ctggggcgtg atcggcgcgg cgcaggcctg cctcgcgcag gtgctcgact
     3301 actcgcagac ccgcatcctg ttcggccggc cgctcgccgc gacgcagacg atccagatcc
     3361 gcctcgcgga gatggcgcgc aggatcacca cggcccagct cctgtcattg cagctcggca
     3421 ggctcaagga cgcgggcacg atgagtccgt cgcacgtctc gctcgccaag tggaacaact
     3481 gccgcatcgc gctcgacgtg gcacgtgatg cacgcgacat cctcggcggt gccggcatca
     3541 gcgcggagta cgtgccgatc cgccacgcgc tcaacctcga gagcgtgatc acctacgaag
     3601 gcaccgagac gatccaccag ctcacgatcg gccgcgagct cacgggcatc aacgcgttct
     3661 gaaggccgcc tgtcggtttc ctttacggat cgccgcggtt ccggcccggc gtgcgccggt
     3721 gaggcacgcg tgcccgaccc taacccattg attcccgggc accacgcggc gcgtggcacg
     3781 tacattgctt ggttttcatc gtcctcgtgt ggtgggtgat gggaaggacg aaggcgctgg
     3841 gtgagggaag agtttccgct gggggattcg ggaacgggaa actcgtgaca cggagagtcc
     3901 tgcgaaggtc cggaggcgca gggcgcgaac aagaagcaga gtgacaacaa atcgaatcag
     3961 cgctccacgg tagacacgga accccgcttc ggcggggttt tttttcgcgt cagccgcccg
     4021 gcgcgcaagt cccccgccgc gccgatctca cacgtccatc acggcgtcgg tcgtcaccca
     4081 gccgccgctc acgttcgcgg ggagcatctg cccgacgtcc tcgccgactt cccggtcgat
     4141 caacgcgttc aacttggcat tcatcgtctc gagcagttcc cgggacctgt cgcgatccag
     4201 cgcgaggttg tctcgctgca gcgggtcgcg ctcgagatcg aacagttcca cgtcgttcag
     4261 cttgaacagc gcttcgatcg atgccgggcg attgtgctgg cgcggcgaga agtagcgggt
     4321 caactggtag cgaccgtcga agacgctgcg gatcgcgccg cgcttgttca ggtccggccg
     4381 cagcgtgccc gccttgacgg cttcctgcag cttcgccttc ccgtcgggct gctgcatgac
     4441 cgccaccacc ttctgcatga agtcaccgtc gatgtaggcg aacatgttgt aacagaacag
     4501 ggcaccgtcc ctggccgagg tgtaggaggc cgtttccggc gcggccagga gcacggagaa
     4561 gtccctgccg ggaagatgtt tcgctaccgc cgccttcttc gcgggcgtgg catctgtcag
     4621 gcccagcagc gtcggcgcga tgtcgaggtg tgaggtcacg gccctgcaac gccgtccgcc
     4681 gggatacgcc ggatgcgcga cgatcagcgg cacgttgttc tgttcgcggt atgccgtggc
     4741 acctttggcg tgcagccggt gcgcaccgtc gagatcgcca tggtcggcgg tcaggatcac
     4801 gatggtgcgt gaggcgagac cgagcgcgtc cagctcgtcc agcagcgcgg cgatctgccg
     4861 atcgacgtcc cgcaggcaat tcaggtagta gttgtgccgc cggcgccagc gccagtcctc
     4921 gttcggtatg tggccggtca gcccgtcatg cgatttcagg taatcggtgt gcgcggcggg
     4981 gcggcctggg gcgtcgagcc gctcgccgta cgtagccggg aggtcgaacg tccattgcct
     5041 gccgtatgag gtgttggctg gctcggggcg gatgtgcgag aggatgttgc gcgcctggac
     5101 cggctcgccg gggcggtccg tgttgaggta catgacgtcg tgggggttaa cgaggttgac
     5161 cgcgaggaac cacggcttgc cctgcgtcgc gagttccgct cccttcccgc gcagccagct
     5221 cacgcccatc gcggaaatga tgccgtcgtg caggtatccg ccctggtcgt gggcgatgat
     5281 gtcgcccacc cctacgtagt ccgagaagcc gtattcctcc atctcccggg tgaagatctt
     5341 cgtgggcgct ccgagctcgt tgaccgtctc gaattccttg gtgaggtgcc acttgccctt
     5401 gtaggccgtg tagtagcccg cttcccgcag gaggtcgccg acggtgggga tgtcggtcga
     5461 catgctcgag atccacggga agttcgtgtt gtcgaacatc ctcgtgtgct gcatgtgctg
     5521 cccggtgtag agcacggagc gcgagggcgt gcacacgcag gagttgatgt ggtggttctc
     5581 gaagaaagtg ccccgctgcg cgaggcgctc gtgcgccggc aagcggtagt cgcctggcag
     5641 ttcgccgggc cggaacaggc gttcctggtc cgtcaggatg aagaggatgt tgtacgggct
     5701 gccggcgggc ccgggcgccg caccgggctg cgggtcattc gccgtcgccg ctgcagcgga
     5761 acccgcggca gcgccgagcg tcgtcgcgac ggcgccggcc ccgatggcct tgagcagatc
     5821 cctgcgcgtg atgtcgactc cgccccgctg ttcgccgttc atggatcgcg cctcggtgcc
     5881 cgggcaaatg aggccgaagc atcggggatc gatgcgagga tcacaagccg ggccgccgaa
     5941 tggctcgtcc gcccggccca ttcggggacc gccgaggccg ctaccggcca tgccgcaggg
     6001 atgtgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngatgg cgtcaggttt
     6121 cttttccgat gttccggaca tgaaactcgt ctgcgacgga gttcgctgcg ccggatctca
     6181 cgtcatctat ctgtggactt tcacggggca cgcagccagg acctggaacg tcatcagcgt
     6241 gcacggctgg gaagaatggg atgtcggcgg cgatttgcag gtaaaggctt ctcgcggttg
     6301 gtacgactcc gacgactacg cacggcaggt ggatggagca accgcctgac cgtctgcatc
     6361 gggtcgcgtt cacgtcgaat cggcatcgcc tcgacgtcgc atcgcggtcg cgcgacgagt
     6421 gacgcgaggc ggcgcgcaac gccgcctact ggactccggc gagacttgaa cgcacaatgc
     6481 ggccgcgcgc ttggcctcaa cgtgcgcgat cgctccgccg cgctgtcctc acccctattc
     6541 gggttaagtc caacggcgcg gcggggcgat gtctccccgc ggaccccgga ttccggtgcc
     6601 gggcacggcc aatcacggac acggccaacg atgacgcaag gcgccgacct accccagatc
     6661 atcgcgcatc gcggcaatgc ggcggagttc ccggagaaca ccctgcaggc actcgcgtcc
     6721 gcggtggaac tcggcgtgaa gcacctcgag ttcgacgtgc agctgacgtc cgaccgcgtg
     6781 ccggtcgtgc tgcacgactc ggatctcgag cgggtcggcg atgtgtccga agtcgtgcac
     6841 gacatgacct gggcgcagct ctccgagacg ccggtcggcg agttcaagcg cttcggcagc
     6901 cggttcgcct atacctaccc gccgtcgctc gcggaggtcg tgcacgcgct cgccggatgg
     6961 gaaggcgtga ccgcgttcgt cgagatcaag cgggcgagcc tgcgacgctt cggccgcgag
     7021 gcggtgttgc gccaggtcgc gcaggtgctg aagccggtgc tcgaccgctg cgtggtcatc
     7081 tcattcgacc tgcccagcgt ccaggtcatg cgccgcatga cgggcgcgcg gatcggctgg
     7141 gtgatctcga gctacggcga cgacacgctg gccgaagcga cgcgaactgc gccggacttc
     7201 ctgttcgcca acctcgagcg cctgccgcag gggctgcagg cactgtggcc cgggccgtgg
     7261 cagtgggcga tctacgaggt ccgtgacatc cggaccgcgc tcgactgcca ggcgctcggc
     7321 gccgccttcg tcgaatcgat ggcagtgcgc ggcctcggcc tcgccttcga ggagtatcgc
     7381 cggcagtggt gagtgcgccg acgccggggc tgatcctggc gctcgaccag ggcggtcacg
     7441 gtagccgcgc cgtgctgttc gacgagacgg gcagcgagct caccgaggcg cacgcgccgg
     7501 tcgccacccg tcacggcgaa aacggctggg tcgagcagga tccgctcgaa ctgctccgct
     7561 ccctgcatgt cgcagcgcgg gacgcttgtg attcacccgc cgcgaccggc cgcccggtga
     7621 tcgcggccgg ccttgccacg caacgctcga cgatcgtctg ctgggagcga tccaccggac
     7681 gcgcgctgac cgatgcaatc tcgtggcagg atcgccgcaa cgcggcgtgg ctcgagcagc
     7741 tgcgtcccgt cgagccggag attcgcaggc taacggggct ggtgctctcc gcgcattacg
     7801 gcgcgagcaa gctccgctgg tgcctcgacc acgtcccggg cgtgcagctc gccgcgcgac
     7861 gcggcgacct ggcattcgga ccgctggcga gcttcctgct cgcgcgcctg ctcgacgagc
     7921 ggccgtcggt cgccgatccg gcgaacgcgt cgcgcaccct gctctacgat cccacgcggc
     7981 tggactggtc accgccattg ctcgacacgt tcggcatcga ccgaagctgc ctccccgcct
     8041 gcgtgccgac gcgccacgcg tacggtcgca tgcgcgtcgg cgcccggcac attccgctca
     8101 cggcgtgcac cggcgaccag tccgcggccg cgctcgcatt cggcgcgccg agccagtccg
     8161 ttgcgctcgt caatgccggc acgggcgcct tcgtccagcg cgccgtcccg gccggagccg
     8221 tgctgccgga tggcctgctg tgcagcgtgc tgcgctccga cggcgtgcac gccacgttca
     8281 gtcacgaagg cacggtcaat ggcgcgggca gcgcggtcga gtggctgggc agtcgcatcg
     8341 cactcgacgt gagacgcgcc atcgcggtgc tggatgtcgg gccgtcggat cgggtgccgc
     8401 tcttcatgaa cggcgtgggc gggctcggct cgccctactg gaaggccaac ttcccggtgg
     8461 agttcgtcgg cgagggaagc gacgcgatga agctcgcggc ggtcgtcgag agcatcgcat
     8521 tcctgctgtg cgccaacctc gagaccatgc gccgcagcgc gccgctcgac ggcatacgga
     8581 tctccggcgg cctcgccagc tccgactacc tgtgccagtc gctcgccgac gcgagccgcc
     8641 tgccggtcga ccgccatgcc ttgcgcgagg cgaccgcgcg aggcgtcgca ttcctcgccg
     8701 ccggggaacc ggccggctgg catgcgccac cactcgaacg cgcattcgtc ccacggcgga
     8761 atgacccgct cgccgcacgc cacgcagcct ggctcggcga gatggcgcgg cgcggggcgt
     8821 gatcagcccc ccgcgagtgc ggcgtacacc tcgcgctcga tccacgtgcc gccccgacga
     8881 ccgtcgtagt agccgcaatg gccgccgaat ctcgtgcggg tcaccgtgag tgccgcggga
     8941 cgcgcgatgc gatcgaagtc ctcgacggga atgatcgggt cgtccgccgc cgtgatgatc
     9001 cgggtcggat gcacgatgct gtcgagagcc tcgccgacga tcgcgtaacc cttgaggtag
     9061 gcctcgagcg tcggataccc gccgtacttg cacgcgaggt ggtccgtcat ttcagtgagc
     9121 gtcttcatgg cccgcacgtc ggagaggtcg tagagttcgg gccacgcgga ctgcttcagg
     9181 cgcagcgagc cgcgccactt ccagacgaaa tagcgccggt agacgggcca gcccagttcc
     9241 aggcgccaca gcgtgtgctc cgggtccacg accgggctta cggcgatcac gcgctcgagc
     9301 tcgatgccgg ccgcccgggc ccgggccgcg acgcgcagga agaaattgcc acccagcgag
     9361 aacccgacca ggttcacgcc ccgcccgcga ttggtgtcct ggatgcgccg cacggcgccc
     9421 acgacttcct tgatcctgca ggagtgaaag atctcggggt tcaggtggtg gctgccgccg
     9481 tgatcgcgga ggttcagccg cacgacgtcg aagccgcggt ccagcaggta ctgcccgagc
     9541 gacaggatgt acagcgagtc ggagctgccc tcccagccgt gcagcagcac cgcgacccgc
     9601 gcggccggcg cgcggccgat ccgttcctgc gtggcgtggt gggcgagcag ccgcacgccg
     9661 tcgccgcaat cgaccagcag ttcctcggac gccgcgcgca gccggcgcgt gtgccacgcg
     9721 accgcgggcc ggcgaaacgg caggctgggg aacaccgact ggacgtgggg atttgcgagc
     9781 cacgccggtg gcgcgaattc gccgaccgtc actccgcagt caccactcgg aagtcgacac
     9841 ggttgttctg cgccgccacg gtgttccacg tgccggcggt cgtggcttcc gtctgctccg
     9901 gatactcgac cggatggcgg cggcccgcac tgaccgactc gaccgtgatg gcgccgccgc
     9961 tgcgacgggc cagcatcgcg acgtaattgg cgaagtcgac cgactggcgc tgtgcggccg
    10021 acagcgaatc gtcgaacggg ttgccgacga tgtcgcactt gcgaatgggc agcgacgggt
    10081 ccgccacttc gaatccctgg gcctggctgc cggcgaggca gaagtcgccg accgagtact
    10141 cgagcacgat gcggccgcgg aagcgctgcg cctcgagcga cgcgaccagg gactgcaggc
    10201 gtgcgagccg cgcgccgccg aacggaatgt caccgtacgg cacgtattcc ggccgcgccg
    10261 gcgcagggac ggcggcggac gacaaggcct gcggctgcgg cgcaccggtg acgtcccccg
    10321 gctgatcggc gggcactgcg acgggcgccg gtgcgagggc cagcggcagg gtcctcgcga
    10381 gcttcgcaac ggagtcctgg agttgcgaga tctgcgcgcc gtaatcgcga ttgaccttga
    10441 gttcctgcca gtagagcagc ccgagcacga gggccgggat gaccgcgagc agggccgtga
    10501 gcgccaccgg ccaccactgg cggggcggtg gctcgggcgc ggggagttcg acgaccgtcg
    10561 gcgtcgccgg cttctgcgcc tgctcgcgga tctccgtgcc gaggcg
//
LOCUS       DAOMFD010000061        10436 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1782_length_10436_cov_31.235526, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000061 DAOMFD010000000
VERSION     DAOMFD010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10436)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10436)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10436
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1782_length_10436_cov_31.235526"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1598)
                     /gene="guaA"
                     /locus_tag="PL152_05320"
     CDS             complement(<1..1598)
                     /gene="guaA"
                     /locus_tag="PL152_05320"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HQR48732.1"
                     /translation="MSAEHPAARPPDIHAHRILILDFGSQYTQLIARRVRELGVYCEI
                     HPWDADEEAVRTFGARGIILSGGPESVTQASAPAAPAAVFEAGVPVLGICYGMQTMAA
                     QLGGRVEPGTVHEFGYAEVRARGHSALLRDIEDRVSAEGHGLLDVWMSHGDRVTELPP
                     GFEVIASTADVPIAGMADEARHLYALQFHPEVTHTRQGARIYERFLHGICGCDSGWNA
                     GNIIEDAVLRVRAQVGASKVLLGLSGGVDSSVVAALLHKAIGDQLTCVFVDHGLLRHH
                     EGDQVMAVFARNLGVRVIRVDAAERFYAALAGVSDPEAKRKIIGRLFIEVFDEEAHKL
                     EGVEFLAQGTIYPDVIESAGSKTGKAHVIKSHHNVGGLPETMKLRLVEPLRELFKDEV
                     RRIGIELGLPAEMVHRHPFPGPGLGVRILGEVTRAAADTLRLADHIFIEELRKAGWYE
                     KTSQAFAVFLPVKSVGVTGDGRRHEPVIALRAVETVDFMTAHWAHLPYELLDVCSRRI
                     VNEVKGVSRVVYDITGKPPATIEW"
     gene            complement(1588..3057)
                     /gene="guaB"
                     /locus_tag="PL152_05325"
     CDS             complement(1588..3057)
                     /gene="guaB"
                     /locus_tag="PL152_05325"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004657166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HQR48733.1"
                     /translation="MLRILEEALTFDDVLLVPAFSEVLPHEVNLATQLTRGIRLNLPV
                     VSAAMDTVTEHRLAIAIAQEGGIGIVHKNMSAEAQARQVARVKRFESGIIHDPITVRP
                     DATIREVLELTRAKNISGVPVVKDGKAVGIVTHRDLRFETRYDAPVSSVMTPRERLVT
                     VRENSPKEEVLALLHKHRIEKVLVIDEDFGLKGMITVKDFQKATEFPRACKDERGALR
                     VGAAVGTAPDTLDRVAALREAGADVIVVDTAHGHSRGVIQTVAKIKARWPAQQVIGGN
                     IVTREAALALVDAGADAVKVGIGPGSICTTRVVAGVGVPQITAVANVAAALAERGVPL
                     IADGGIRFSGDVAKAIVAGAYCVMIGGLFAGTEESPGEVELYQGASYKSYRGMGSLGA
                     MAQAHGSRDRYFQDTATELEKLVPEGVEGRVPYKGSIVTILHQLAGGLRAAMGYTGSG
                     SIEQMRTVPRFVRITNAGMRESHVHDVAITKEAPNYRVS"
     gene            3174..4538
                     /gene="xseA"
                     /locus_tag="PL152_05330"
     CDS             3174..4538
                     /gene="xseA"
                     /locus_tag="PL152_05330"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HQR48734.1"
                     /translation="MFDLRHDGDGNPPPPRDIFTVSRLNREARMLLERALGSVWLEGE
                     MSNLSRPASGHWYFSLKDESAQVRCAMFRQRNLLVRFPVKDGAHVVARGRVGLYEPRG
                     EFQVVVEHLEEAGEGLLRRRFEELKARLGAEGLFDERYKQALPRLPGRIGVITSPTGA
                     ALRDILHVLRRRFPAVPVLVYPVAVQGEAAPREIEQALRLAATRRDCDVLIVARGGGS
                     LEDLWAFNDESVARAMHACPIPIVSGVGHETDFTIADLVADERAPTPSGAAERVVPDR
                     AEWLRGFAAGQRRAQLAAARRLGDLGSLLRATSARLARVHPGVRLRQHAQRLDELDTR
                     LRRAVRSELEGWRSRTTTAVALLLKASPAIRVQAQRVRLESAARALGAAARAGLAMAR
                     SRFDLAARTLHAVSPLATLDRGYAIVTDSGGHVLLDASSVAHGDHIEARLAHGRVRAT
                     VVAK"
     gene            4573..5445
                     /locus_tag="PL152_05335"
     CDS             4573..5445
                     /locus_tag="PL152_05335"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQR48735.1"
                     /translation="MSPISVAALICVAVVALAASLAAAATNLPEERRVPGGIALIDLG
                     ATDAPGAVYFNDHRAPVLPTERGWVAVIGIPLDTKPGRHAASYRPNGTTQVGSGPARR
                     TSLEFTVADRKYAEQRLKVTNEHHVNPDAQDLARIDREQLRIDAALGTYTAGRVPDLR
                     MQPPIAGERSSSFGLRRFFNGEARKPHSGMDIAAGLGTPIHAPSPGRVLDTGDFFFNG
                     NTVFVDHGEGVVTMYCHLSRIDVQPGQDVATGDVLGLVGATGRVTGPHLHWGIAVNRA
                     MVDPALFLAGPPGE"
     gene            complement(5470..6636)
                     /locus_tag="PL152_05340"
     CDS             complement(5470..6636)
                     /locus_tag="PL152_05340"
                     /EC_number="2.3.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF001209.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase"
                     /protein_id="HQR48736.1"
                     /translation="MAMALARTPPTTVADLPARRLARLGSPLRLLRGGVIEHPIVAYE
                     AWGTLNEARDNAIVIFTGLSPSAHAASSPDDARPGWWETMIGPGRPLDTRRFFVVCIN
                     SLGSPFGSSSPASLDPASGRPYGIAFPEIAVEDIASAGREALRSLGIERVAALVGPSL
                     GGMVVLAYCALFPGEVEHLLTISGAARATPFAIALRSLQREMVRSDPAWRGGNYEPGR
                     GPRLGLRLARKLGTITYRSPQEWDQRFGRMRMTDTVGLSGDFRPQFEVEGYLEHQAKR
                     FSESFDANCYLYLSRAMDQFDLADHGGGSLAAAFARFGVRRSLVIGVESDMLFPLHQQ
                     QEVADHLRAAGGIVEYHAFPSLQGHDAFLSDLARFEPAIGGFIAGIPGRPAIAG"
     gene            complement(6746..8155)
                     /gene="der"
                     /locus_tag="PL152_05345"
     CDS             complement(6746..8155)
                     /gene="der"
                     /locus_tag="PL152_05345"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HQR48737.1"
                     /translation="MLPVVALVGRPNVGKSTLFNAITRTRDALVADVPGLTRDRKYGY
                     ARVGPRPCVVVDTGGLVETTQGVERLMAEQTLKAVEEADRVLLVVDARQGCVPADHHV
                     ADVLRRSGRPVIVAANKAESLDPEIAGVDFHQLGFGAPAAVSAAHGEGVEDLLQRALE
                     GLPEPAPAADADATTGAEIRVAVVGRPNVGKSTLINRLLGEERLVAFDQPGTTRDTVF
                     VPFERDAQRYVLIDTAGVRRRARVSDAIEKFSIVKTLQAIEEANVVIGVLDAHDTVAE
                     QDASLLGLVAEQGRALVIAVNKWDGIPPAQRDSIRDGVDLRLDFLAYAPVHFISARHG
                     TGVGELMVAVREAYRAAMKELPTKDLTRALEQAIAMHQPPLVRGRRIKLRYAHQGGRN
                     PPVVVIHGNQTQHVPDAYRRYLENHFRQVFRLTGTPMRIEFRTDENPYSGRRNELTPR
                     QQRKRVRLIKNRRAGTGKR"
     gene            complement(8158..9315)
                     /gene="bamB"
                     /locus_tag="PL152_05350"
     CDS             complement(8158..9315)
                     /gene="bamB"
                     /locus_tag="PL152_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR03300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HQR48738.1"
                     /translation="MTTLRLLPALVLLAALAACNKDTTVEPPAELVDIKPTLAVQKLW
                     SEGMGGGGEVLRLTLGVAGMGDTVYAAGHDGKVRALDAASGKTRWQVDTKLDLSAGPG
                     VGAGLVVVGTSDGDLVALEAATGKQRWKTRIPGEILAPPLIDGDHVLVRSVEGRLRSL
                     SAADGKQEWMVEDLVPRLSLRGTATPVAASGDVIVCGFDTGKLMAVTLAKGELLWQMQ
                     LDTPRGRTELDRLADVDAAVQVSGKDVYAVGYHGRVAMISLDNGQVWWSRDMSSYRGL
                     AIDDDQVYVSTSEGDVVALRRRDGTVVWQQRGLARRTLSTPAVDGNAVVVGDFEGYLH
                     WLDRSSGKFVAREHPGDSRIAAPPLVMGGRLFVIDEGGHLVAYRSGGAAGS"
     gene            complement(9312..10013)
                     /locus_tag="PL152_05355"
     CDS             complement(9312..10013)
                     /locus_tag="PL152_05355"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR48739.1"
                     /translation="MEDLSDNEREEQLRRWWGENWLWIVGGIALGMALLWGWQFWQRS
                     RLEQAQQDDAAYMAVINLLDQRKFDDAAAQAKALRDRHPTSPYSDQAELALARAAVEA
                     RKLDDAAQHLRVVMDGSKDAELRTVARTRLARVLIEQGKFDDAVTLLDLQDANSFAAL
                     YRELRGDAYAAKGDATAARREYDAALSPQYAESGVDRAYVELKRDALPVGASSAPAAP
                     ASAPPAAAAPGANGQ"
     gene            complement(10066..>10436)
                     /locus_tag="PL152_05360"
     CDS             complement(10066..>10436)
                     /locus_tag="PL152_05360"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HQR48740.1"
                     /translation="GEERIVELLRLQGRAGDESTADVYLVLGGAAAEARGFVLAEALR
                     DALPGARIETNCGGGSFKSQLKRADRSGARYAVILGDEELARGVAALKPLREDTGQAE
                     VALEDLAVEMSRRVPGRARD"
BASE COUNT         1496 a         3786 c         3593 g         1561 t
ORIGIN      
        1 tcccactcga tcgtcgccgg cggtttgccg gtgatgtcgt agacgacgcg cgagacgccc
       61 ttcacctcgt tgacgatgcg gcgcgagcag acgtcgagca gctcgtacgg caggtgcgcc
      121 cagtgcgcgg tcatgaaatc cactgtctcg acggccctga gcgcgatcac gggctcgtgg
      181 cgccggccgt cgccggtgac gcccacgctc ttcacgggca ggaacaccgc gaaggcctgg
      241 ctcgtcttct cgtaccagcc ggccttgcgc aattcctcga tgaagatgtg gtccgcgaga
      301 cgcagcgtat cggccgcggc gcgggtcacc tcgccgagga tccgcacgcc gaggcccggg
      361 cccgggaacg ggtggcgatg gaccatctcc gcaggcaggc cgagctcgat gccgatgcgc
      421 cgtacctcgt ccttgaacag ctcgcgcagc ggctcgacga gcctgagctt catcgtctcc
      481 ggcaggccac ccacgttgtg gtgcgacttg atgacgtgcg ccttgccggt cttgctgccg
      541 gccgactcga tcacgtcggg atagatggtc ccctgcgcga ggaactccac gccctcgagc
      601 ttgtgagcct cctcgtcgaa gacctcgatg aacaggcggc cgatgatctt gcgcttggcc
      661 tcggggtccg agacgcccgc gagcgccgcg tagaagcgct cggcggcgtc cacgcggatg
      721 acgcgtacgc cgaggttgcg cgcgaacacc gccatgacct ggtcgccctc gtggtgacgc
      781 aacagcccgt ggtccacgaa cacgcaggtg agctggtcgc cgatcgcctt gtgcagcagc
      841 gccgcgacga cggaggagtc gacgccgccc gacaggccga gcagcacctt gctggcgccc
      901 acctgcgcgc gcacgcgcag gacggcatcc tcgatgatgt tgccggcgtt ccagcccgag
      961 tcgcagccgc agatgccgtg caggaaccgc tcgtagatgc gcgcgccctg ccgcgtgtgc
     1021 gtgacctcgg ggtggaactg cagcgcgtat aaatgccgcg cctcgtcggc catgcccgcg
     1081 atcggcacgt cggcggtcga ggcgatgacc tcgaagccgg gcggcagttc ggtgacgcgg
     1141 tcgccgtggc tcatccacac gtcgagcagg ccgtgcccct cggcactgac tcggtcctcg
     1201 atgtcgcgca gcagcgcgga gtggccgcgc gcgcgcacct ccgcgtagcc gaattcgtgc
     1261 actgtgcccg gctcgacacg ccccccgagc tgcgcggcca tggtctgcat gccgtagcag
     1321 atcccaagca ccgggacgcc ggcctcgaac acggccgcgg gcgccgcggg cgcactggcc
     1381 tgcgtgaccg actccgggcc gcctgacagg atgatgccgc gcgcgccgaa cgtgcggacc
     1441 gcctcctcgt ccgcgtccca cgggtggatc tcgcagtaga cgccgagctc gcgcacgcga
     1501 cgcgcgatca gctgcgtgta ctgcgaaccg aagtcgagga tcaggatccg gtgcgcgtgg
     1561 atgtcgggcg ggcgggcggc cggatgctca gctgacacgg tagttcggcg cttccttggt
     1621 gatggccacg tcgtgcacgt ggctctcgcg catcccggcg ttggtgatgc gcacgaaacg
     1681 cggcaccgtg cgcatctgct cgatgctgcc actgccggta tagcccatgg cggcgcgcag
     1741 gccgccggcc agctggtgca ggatggtgac gatgctgccc ttgtagggca cgcggccctc
     1801 gacgccctcc ggcacgagct tctcgagctc ggtcgcggtg tcctggaaat agcggtcccg
     1861 cgagccgtgg gcctgcgcca tcgcgccgag cgagcccatg ccgcggtacg acttgtagga
     1921 ggcgccctgg tacagctcca cctcgccggg cgactcctcg gtaccggcga acagcccgcc
     1981 gatcatgacg caataggcgc cggcgacgat cgccttggcg acgtcgccgg agaaacggat
     2041 gccgccgtcg gcgatcagcg gcacgccgcg ctcggcgagc gccgcggcca cgttcgccac
     2101 cgccgtgatc tgcggcacgc ccacgccggc gacgacgcgc gtcgtgcaga tcgagcccgg
     2161 gccgatgccc accttgaccg cgtcggcgcc tgcatccacg agggcaaggg ccgcctcgcg
     2221 cgtgacgatg ttgccgccga tgacctgctg cgccggccac ctcgccttga tcttcgcgac
     2281 ggtctggatc acgccacgcg aatggccgtg cgccgtgtcc acgacgatca cgtccgcgcc
     2341 cgcctcgcgc aacgccgcga cgcgatcgag cgtgtccggg gccgtaccca cggcggcccc
     2401 gacgcgcagc gccccgcgct cgtccttgca cgcccgcgga aactccgtgg ccttctggaa
     2461 atccttgacg gtgatcatgc ccttgaggcc gaagtcctcg tcgatcacca gcaccttctc
     2521 gatgcggtgc ttgtgcagca gcgccaggac ctcctccttc ggcgagttct cgcgcaccgt
     2581 caccagtcgc tcgcgcgggg tcatcaccga cgagaccggc gcgtcgtacc gcgtctcgaa
     2641 ccgcaggtcc cggtgggtga cgatgcccac cgccttgccg tccttgacga ccggtacgcc
     2701 ggaaatgttc ttggcgcgcg tgagctcgag cacctcgcgg atcgtggcgt cgggacgcac
     2761 cgtgatcggg tcgtggatga tcccgctctc gaagcgcttg acccgggcca cctggcgggc
     2821 ctgtgcttcg gcgctcatgt tcttgtgcac gatgccgatg ccgccttcct gcgcgatcgc
     2881 gatcgcgagc cggtgctcgg tgaccgtgtc catcgcggcg gaaacgaccg ggagattcag
     2941 gcggatgccc cgggtgagct gcgtggcgag gttcacttcg tgcggcagga cctcggaaaa
     3001 ggccggcacg agcaggacgt cgtcgaaggt gagggcttcc tctaggatgc gcagcatggg
     3061 ctgtcccggg cttgaaaaag gaagcggaca attgtacccg gcggcgcggc gcgggggaaa
     3121 cccgggtggc gctccttgtg cccaccggcc gggccgctaa gatcgctgcc accatgttcg
     3181 atctccgcca cgacggcgac ggcaaccccc cgccgccacg cgatattttc accgtcagcc
     3241 gcctcaatcg cgaggcgcgc atgctgctcg agcgggcctt gggctcggtg tggctcgaag
     3301 gcgagatgtc caatctgtcg cgtcccgcgt ccggccactg gtacttctcg ctcaaggacg
     3361 agtccgcgca ggtccgctgc gccatgttcc gccagcggaa cctcctggtg cgcttcccgg
     3421 tcaaggacgg cgcgcacgtg gtcgcgcggg ggcgcgtcgg cctctacgag ccacgcggcg
     3481 agttccaggt ggtcgtcgag cacctcgagg aggccggcga aggcctgctg aggcgccggt
     3541 tcgaggaact caaggcgcga ctgggggccg agggcctgtt cgacgagcgg tacaagcagg
     3601 ccctgccccg cctccccggg cgcatcggcg tcatcacctc gccgacgggc gctgcgctgc
     3661 gcgacatcct gcacgtgctg cggcggcgat ttccggccgt ccccgtcctg gtctacccgg
     3721 tcgccgtgca gggtgaggct gcgccgcgcg agatcgagca ggccctcagg ctcgccgcga
     3781 cgcgccgcga ctgcgacgtc ctcatcgtcg cccgcggcgg cggctccctc gaggacctct
     3841 gggcattcaa cgacgagtcc gtcgcgcggg ctatgcacgc atgcccgata ccgatcgtga
     3901 gcggcgtggg tcatgaaacc gacttcacga tcgcggatct cgtcgccgac gaacgcgcgc
     3961 caacgccttc cggcgctgcg gagcgcgtgg tgcctgatcg ggcagagtgg ctgcgcggat
     4021 tcgctgcggg gcagcgtcgc gcgcaactcg ccgcggcgcg ccgactcggc gacctgggct
     4081 cgctgctgcg ggcgacgtct gcgcggctgg cccgcgtgca tcccggcgtg cgactgaggc
     4141 agcacgccca gcgcctcgac gaactcgaca ctcgactgcg ccgcgcggtg cggtccgaac
     4201 ttgaaggatg gcggtcccgc acgacgacgg cggtcgcatt gctgctcaag gcatcgccgg
     4261 ccatccgggt gcaggcgcaa cgtgtgcgcc tcgagtccgc ggcgcgcgcc ctcggggcgg
     4321 ccgctcgcgc cggactcgcc atggcccgca gtcgcttcga tcttgccgcc cgcacgctgc
     4381 acgccgtgag cccgctggcc acgctggatc gcgggtacgc gatcgtcacg gacagcggcg
     4441 ggcacgtcct gctcgacgct tcgtcggtgg cgcacggcga ccacatcgag gcgcggctcg
     4501 cccatggccg ggtccgtgcc accgtcgtcg ctaaataggg gacattctcc tatttcgact
     4561 cgaaatagga gaatgtcccc tatttctgtt gcagccctga tctgcgtggc agtcgtcgcg
     4621 cttgccgcca gcctggcggc cgccgcgacc aaccttcctg aggaacggcg cgtacccggc
     4681 ggcattgcgc tcatcgacct gggagccacg gacgcgccgg gcgccgtgta cttcaacgac
     4741 caccgcgccc cggtgctgcc gacggagcgc ggctgggtcg ccgtgatcgg cattcccctc
     4801 gacacgaagc ccgggcgaca cgcggcgagt tatcgaccga acggcacgac gcaggtcggg
     4861 agcggaccgg cacgccgcac gtctctcgag ttcaccgtcg ccgacaggaa gtacgccgag
     4921 cagcgcctga aggtgaccaa cgagcatcac gtgaaccccg acgcacagga cctcgcgcgg
     4981 atcgatcgcg agcagctgcg catcgacgcc gcgctcggca cctacacggc cggcagggtc
     5041 ccggacctgc gcatgcagcc cccgatcgcc ggcgaacgct ccagcagctt cggattgcgg
     5101 cggttcttca acggcgaggc gcgcaagccg cacagcggca tggacatcgc cgccggcctc
     5161 gggaccccca tccacgcgcc gtcgccgggc cgggtgctcg acacgggcga tttcttcttc
     5221 aatggcaaca cggtgttcgt cgaccacggt gaaggcgtcg tgaccatgta ctgccacctg
     5281 agtcgcatcg acgtgcagcc tggccaggac gtcgcgaccg gcgacgtcct cgggctggtc
     5341 ggcgcgaccg ggcgagtgac cggaccgcac ttgcactggg gcatcgccgt gaatcgggcg
     5401 atggtggacc cggcgctgtt cctggccggg ccgccgggag agtgagcggt cgcgggcgct
     5461 cgccggccgt tatccggcga tcgcggggcg acccggaatc ccggcgatga atccgccgat
     5521 tgccggctcg aagcgcgcga ggtcgctcag gaatgcatcg tgcccctgca gcgacgggaa
     5581 cgcgtggtat tcgacgatcc cgccggccgc gcgcaggtga tccgcgactt cctgctgctg
     5641 gtgcagcggg aacagcatgt ccgactcgac gccgatcacg agcgagcgtc ggacgccgaa
     5701 gcgcgcgaac gcggccgcga gcgacccgcc gccgtggtcc gccaggtcga actggtccat
     5761 ggcccgcgac aggtacaggt agcagttcgc gtcgaacgac tcggaaaacc gcttggcctg
     5821 gtgctcgagg tagccctcga cctcgaactg cggccggaaa tcgccggaca gtccgacggt
     5881 atccgtcatc ctcatgcggc cgaagcgctg gtcccattcc tggggcgaac gataggtgat
     5941 ggtgccgagc ttgcgtgcca ggcgcagccc gagtcgcggc ccgcggcccg gttcgtagtt
     6001 gccgccgcgc caggccggat cgctgcgcac catctcgcgc tgcagcgacc gcaacgcgat
     6061 tgcgaacggc gttgcgcgag cggcgccgga aatcgtcaac aggtgctcga cctcgcccgg
     6121 gaagagcgcg cagtaggcca gcacgaccat gccgcccagg gaggggccga ccagcgcggc
     6181 gacgcgctcg atgcccaggc tgcgcaatgc ctcgcgcccc gccgaggcga tgtcctcgac
     6241 ggcgatctcc gggaaagcaa tgccgtaggg tcgccctgag gctgggtcca gcgacgccgg
     6301 gctcgacgac ccgaacgggc tgcccagcga attgatgcac accacgaaga atcggcgcgt
     6361 gtcgagcggc cgcccggggc cgatcatggt ctcccaccag ccgggccgcg catcgtcggg
     6421 cgatgacgcc gcatgcgcgg atggcgacag gcccgtgaag atcacgatcg cgttgtcgcg
     6481 cgcctcgttc agggtgcccc aggcctcgta cgccactatc gggtgctcga tgacaccgcc
     6541 gcggagcagg cgcagcggcg agcccagccg ggccagcctg cgcgcgggca ggtcagcgac
     6601 tgtcgtcggc ggcgtacgcg cgagtgccat ggccatgcgg gcagtctatg cccgcctccc
     6661 cgctgaacaa gtgccagagg atagcgcgtc agaagcagta gttatgagcg aggcgcgccc
     6721 ggccgggtga ggccccgccg ccggctcagc gcttgccggt acccgcacgc ctgttcttga
     6781 tcaggcgcac gcgcttgcgc tgctgccggg gggtcaactc gttgcgccgg cccgagtacg
     6841 ggttttcgtc ggtgcggaac tcgatacgca tcggcgtgcc ggtcagccgg aacacctgcc
     6901 ggaagtgatt ctcgagatag cgccggtagg cgtccggcac gtgctgggtc tggttgccgt
     6961 ggatcaccac gaccggcgga ttgcgccctc cctggtgcgc atagcgcagc ttgatgcgcc
     7021 ggccccgcac caggggcggc tggtgcatgg cgatggcctg ctcgagcgcc cgggtcaggt
     7081 ccttcgtcgg cagttccttc atggccgccc ggtaggcctc gcgcaccgcg accatcagtt
     7141 ccccgacgcc cgttccgtgg cgcgccgaga tgaagtgcac cggtgcatag gcgaggaaat
     7201 ccaggcgcag gtcgacgccg tcgcggatcg agtcgcgctg tgccggaggg atgccgtccc
     7261 acttgttgac ggcgatcacc agtgcgcgtc cctgctccgc gaccaggccg agcaggctcg
     7321 cgtcctgctc tgcgaccgtg tcgtgcgcgt cgagcacgcc gatcacgacg ttggcctcct
     7381 cgatcgcctg cagcgtcttc acgatgctga acttctcgat cgcgtcgctc acgcgcgcgc
     7441 ggcgccgcac accggctgtg tcgatcagga cgtacctctg ggcgtcgcgc tcgaacggca
     7501 cgaacaccgt gtcgcgcgtc gtgcccggct ggtcgaaggc gacgagccgt tcctccccga
     7561 gcaggcgatt gatgagcgtg gacttgccga cgttcgggcg accgacgacg gccacgcgga
     7621 tttccgcgcc cgtcgtggca tcggcatccg ccgctggcgc gggctccggc aggccttcga
     7681 gcgcgcgctg caacaggtcc tcgacgccct cgccgtgggc ggccgatacg gccgcaggcg
     7741 cgccgaagcc gagctggtgg aagtccacgc cggcgatctc ggggtccagc gattccgcct
     7801 tgttcgccgc cacgatcacc ggacgcccgc tgcgccgcag gacgtccgcg acgtgatggt
     7861 ccgcgggcac gcagccctgg cgggcgtcga cgaccaggag cacgcggtcg gcctcctcga
     7921 ccgccttgag ggtctgctcc gccatgaggc gctccacgcc ctgcgtcgtc tcgacgagtc
     7981 cgccggtatc gaccacgacg caaggccgcg gccccacgcg cgcgtagccg tacttgcggt
     8041 cgcgcgtgag cccgggcacg tcggcgacga gcgcgtcccg ggtgcgggtg atcgcgttga
     8101 agagcgtgga cttgccgacg ttcgggcggc cgacgagggc gacgacgggt agcacggtca
     8161 gctcccggca gcgcccccgc tgcggtaggc caccagatgg ccgccttcgt cgatcacgaa
     8221 caggcgcccg cccatgacga gcggcggcgc ggcgatgcgc gagtcgcccg ggtgctcgcg
     8281 cgccacgaac ttgcccgagc tgcggtcgag ccagtggagg tagccctcga agtcaccgac
     8341 gaccacggca ttgccgtcga ccgccggcgt gctgagcgtc cggcgcgcga gtccgcgctg
     8401 ctgccatacc acggtgccgt cgcggcggcg cagcgccacg acgtcgccct cggacgtcga
     8461 gacatagacc tggtcgtcgt cgatcgcgag accgcgatag ctcgacatgt cgcgcgacca
     8521 ccagacctgg ccgttgtcga gcgagatcat cgcgacccgg ccgtggtagc cgacggcgta
     8581 cacgtccttg cccgacacct gcacggccgc atcgacgtcg gcgaggcggt cgagctcggt
     8641 gcggccgcgc ggggtgtcga gctgcatctg ccacagcaac tcgcccttcg ccagcgtcac
     8701 cgccatgagc ttgccggtgt cgaaaccaca cacgatcacg tcgccgctgg ccgcgacggg
     8761 cgtggcggtg ccgcgcagcg acagccgcgg aaccaggtcc tcgaccatcc actcctgctt
     8821 gccgtcggcg gccgacagcg agcgcaggcg tccctcgacc gagcgcacga gcacgtggtc
     8881 gccgtcgatc agcggcggcg ccaggatctc gcccgggatg cgcgtcttcc agcgctgctt
     8941 gccggtcgcg gcctcgagcg cgacgagatc accgtcgctc gtcccgacca cgaccaggcc
     9001 cgccccaacg cccggccccg cagacaggtc gagcttggtg tccacctgcc agcgggtctt
     9061 gccgctggcc gcgtcgagcg cgcggacctt gccgtcgtgc ccggccgcgt acaccgtgtc
     9121 acccatgccc gcgacgccga gcgtcagccg caacacctcg ccgccgccgc ccatcccctc
     9181 cgaccagagc ttctgcaccg ccagggtcgg cttgatgtcg acgagttccg ccggcggctc
     9241 caccgtggtg tccttgttgc acgccgcgag cgcagcgagc aacacgagcg cagggagcag
     9301 gcgcagcgtg gtcattgccc gttcgctccc ggcgcagccg ccgcgggcgg cgcgctggcc
     9361 ggcgcagcgg gcgcggacga cgcaccgacc ggcagcgcgt cgcgcttcag ttcgacgtac
     9421 gcgcggtcga cgccggactc cgcgtactgc ggcgacaggg ccgcgtcgta ctcgcgtcgc
     9481 gcggccgtcg cgtcaccctt ggccgcgtac gcatcgccgc gcagctcgcg gtacagggcc
     9541 gcaaaagaat tcgcatcctg caggtccagc aacgtgaccg cgtcgtcgaa cttgccctgc
     9601 tcgatcagga cgcgcgcgag ccgcgtgcgg gcgacggtgc gcagttcggc gtccttggag
     9661 ccatccatga cgacccgcag gtgctgcgcg gcgtcatcga gcttgcgcgc ttcgacggca
     9721 gctcgcgcca gcgccagttc ggcctggtcg gagtacggcg aggtcggatg gcggtcgcgc
     9781 agcgccttcg cctgtgccgc cgcgtcgtcg aacttgcgct ggtcgagcag gttgatcacc
     9841 gccatgtagg ccgcgtcgtc ctgctgcgcc tgctcgagcc ggctgcgctg ccagaactgc
     9901 cagccccaca gcagggccat gccgagcgcg atgccgccga cgatccacag ccagttctcg
     9961 ccccaccagc gtcgcagttg ttcttcgcgt tcgttgtcgc tcaggtcttc cacttcgaaa
    10021 actcccgtgc cttcccggcg cccgccggtg tccggcacat tgacgtcaat cgcgggcgcg
    10081 cccagggacg cgtcgcgaca tttccacggc gaggtcctcg agcgcgacct ccgcctggcc
    10141 ggtgtcctcg cgcaacggct tcagcgccgc cacgccgcgc gcgagctcct cgtcgccgag
    10201 gatcaccgcg taacgcgctc cgctgcgatc ggcgcgcttc agctgcgact tgaagctgcc
    10261 acctccgcag ttcgtctcga tccgcgcgcc cggcagcgcg tcgcgcaggg cctcggcgag
    10321 cacgaatccc ctcgcctcgg cggccgcacc gccgaggacc aggtacacgt ccgccgtcga
    10381 ctcgtcgccg gcccgcccct gcaggcgcag caactcgacg atgcgctcct cgccca
//
LOCUS       DAOMFD010000062        10337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1808_length_10337_cov_25.365785, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000062 DAOMFD010000000
VERSION     DAOMFD010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10337
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1808_length_10337_cov_25.365785"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            311..1789
                     /locus_tag="PL152_05365"
     CDS             311..1789
                     /locus_tag="PL152_05365"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HQR48741.1"
                     /translation="MSRFSTRTVLGLGVAMALAAGSASATNGYFTHGVGTKSKALAGA
                     GSANPEEILSIATNPAGLALLPETVDAGLGFFSPSRQYKTSESMLNGNCFIPEGSETP
                     FCPFTIGPNNLQSGSELFLVPYVAMNWKLDEQSALAVAFYGRGGMNTNWQGGTATYDP
                     DGYCIPDVQGCAYGPMTFHGTYGSGSLVTPYGHPAQAGDAGVDLMQAFLNTTYAWKSA
                     DDQFAVGVSAIFAMQLFQARGVGAFAPYTKTYIDAYMQTGMPAMPKNLSGNGHDSSYG
                     FGGSVGGIWNINKMFSVAAAYTSKMFMSKLSDYSDLFAEGGSFDIPSTMTLGVTVRPT
                     EQWAASFDYQTIWYSDTPSVSNPIQKLFDCPTVGGSKPQNCLGGNNGPGFGWKDMDIY
                     KVGLEWRYDDAWTYRLGFSVPDSQPIPKNQMEFNILAPGVVEQHYTAGFTHTLEGGDQ
                     FSVAVMYAPTVSQKGVNNFDPTQTIQFSMHEFELEVAYSWKR"
     gene            1886..2938
                     /locus_tag="PL152_05370"
     CDS             1886..2938
                     /locus_tag="PL152_05370"
                     /inference="COORDINATES: protein motif:HMM:NF015558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HQR48742.1"
                     /translation="MQVIPGLVLAFVLAVLGNYLADAIAVALGLGPGAISGIMVAILL
                     GLAMGNLFRLPASLKPGIDVAVKRVLRIGIVLLGLKLSIVEVGAIGLKSLPVILVTIP
                     TAILLVTFLGRRLGLPDRLGTLIAVGTGICGNTAIVAVSPTIAAKEEETSYAVACITV
                     FGLFAMLAYPFVAHWLFDGDPFRAGLFLGSSVHDTSQVAGAGMVYQDYYHDPQALNVA
                     TVTKLERNLSMLIVIPLMSILYHRRSSEGTEPPPWWTMVPMFVVGFAAMSALRTVGDL
                     GSRPFGLLTADQWHEVVKQASHVTSYCLAIAMAGVGLGTRIGNLRSIGLKPLGLGLVS
                     ALIVGLVSTLLIRTLY"
     gene            2999..4207
                     /locus_tag="PL152_05375"
     CDS             2999..4207
                     /locus_tag="PL152_05375"
                     /inference="COORDINATES: protein motif:HMM:NF012401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR48743.1"
                     /translation="MDRRSFVRGVASATALAGGQLLLPRSLLADGLPSGALESEQMVA
                     LPGKQPLIKRSFRPPNFETPVGMFSDVITPNDRFFVRWHLASIPEIDPAAWRLSIGGD
                     AASAPYELTLAQLQNDFEQVEVVAVCQCSGNRRGLSDPHVPGVQWGYGAMGNARWKGV
                     RLKDLLAKAGITKDAVEVAFDGADRGALDATPDFVKSIPAWKAMDGNAIVAWEMNGGP
                     LPHWNGAPARVVVPGWTATYWMKKVVAIRAVSQPVASFWMAKAYRIPKGKFPTVDRFL
                     TQETPENTPITEIVVNSLITNLTDGQKVRADTLVTVKGIAWDAGYGIRSVDVSVDGGL
                     GWHSAQLGTDIGRYSFREWQYVFKARKGEHVVSARASNGVGETQVDQLIFNAAGYNNN
                     VIQRIGIKAV"
     gene            4219..4512
                     /locus_tag="PL152_05380"
     CDS             4219..4512
                     /locus_tag="PL152_05380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48744.1"
                     /translation="MKATHLLVLLVGWAAADVALADESQIRMKEAPETPTLVANCSGC
                     HSLDYVQMNSKFMKRATWEAEVHKMVAVMGAPVSEADQAALVEYLTREYGVAD"
     gene            complement(4541..5218)
                     /locus_tag="PL152_05385"
     CDS             complement(4541..5218)
                     /locus_tag="PL152_05385"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR48745.1"
                     /translation="MRWSDLTLNKKLGAIAVALGAVALASQPHRGPFVKLDARELASI
                     VAKEVDHVTPTELAGWIIEGRADYRLLDLRNEHEFARYHIPTAENVPLATIADFVPQP
                     TEKIVLYSEGGIHAAQAWMLMRARGYTNVYTVLLGLDGWKDDVLFPVMPADASPLDRA
                     RFERTAAVARFFGGQPRSAGTAPEAEPGLPQAMPPPAAEASFPARAAGTGRPAAPMTP
                     RKKKEGC"
     gene            complement(5215..5805)
                     /locus_tag="PL152_05390"
     CDS             complement(5215..5805)
                     /locus_tag="PL152_05390"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQR48746.1"
                     /translation="MMTPLYKYGYFGDELSLIVAVLIGIGFGFCLERAGFGNARKLAA
                     QFYLFDMSVFKVMFTAIVTALLGVTYLGWLGWLDLSQVYLVPTYLWPQIVGGLVLGVG
                     FVVGGYCPGTSAAAVATGRIDGVLYALGIFAGTFVYGEVYPSIKGFVNADEMGQVTLP
                     EVLGLPWGVIVFAVVLMAVGGFWGATKVERWARDRA"
     gene            complement(5802..6362)
                     /locus_tag="PL152_05395"
     CDS             complement(5802..6362)
                     /locus_tag="PL152_05395"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HQR48747.1"
                     /translation="MNSVPKDTTRNGVESDRGSGYANPYLAGIGLGLVLLAAFVVMGR
                     GLGASGAFSSTVAWLTNLVAPAHAQANEYFQGYLGDGTTHPLKAWLVFEVIGVMAGAL
                     LSGLLAGRVHATVEKGPRVSTGVRLACAFAGGMLMAFGAHLARGCTSGQALTGGALLN
                     VGSWAFMIMVFVGGYAVAWFMRWQWR"
     gene            complement(6398..7333)
                     /gene="nrfD"
                     /locus_tag="PL152_05400"
     CDS             complement(6398..7333)
                     /gene="nrfD"
                     /locus_tag="PL152_05400"
                     /inference="COORDINATES: protein motif:HMM:NF025940.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysulfide reductase NrfD"
                     /protein_id="HQR48748.1"
                     /translation="MLELVTTRHNPLVDPTLHVWGWQIPVYLFLGGWVAGCMVLSGWF
                     MRQGRTPAGRTVAYVLPWAGIVLLSLGMGALFLDLEHRLYVWRLYLTLQPTSPMSWGG
                     WILLLVYPVLALGALAGVPEGLLSRWPALASLRRWQLDADRLRRLGTVNIVAGSALGI
                     YTGILLSSLGARPLWASAVLGPLFLVSGLSTGAAFAHMVARDATERATLLRADVLFMT
                     VELALIALFLIGHLTSGAQHATAVALLLGGPYTAAFWVFVVGLGILLPLAIQSLMVSH
                     RIRHTVIAPLLVMAGGFALRYVIVAAGQYSHWISP"
     gene            complement(7338..7877)
                     /locus_tag="PL152_05405"
     CDS             complement(7338..7877)
                     /locus_tag="PL152_05405"
                     /inference="COORDINATES: protein motif:HMM:NF024644.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HQR48749.1"
                     /translation="MARFGMVIDTRKCVGCMDCVVACKTENDVPDGHCRDWIVQRLQG
                     TMPELRLTITSERCNHCDEPPCVSCCPTGASHVHDYGKVVLVDHGKCIGCKACLAACP
                     YDARFIHPDGYADKCTFCIHRVEQGLDPACVSVCPTHSMTFGDLDDPDSEVSRQIASR
                     RHFVLKPDAGTKPRIHYLE"
     gene            complement(7881..10073)
                     /locus_tag="PL152_05410"
     CDS             complement(7881..10073)
                     /locus_tag="PL152_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR48750.1"
                     /translation="MSHISRRRFLKIAGLGAGATALAAAGARTLQAVTRDGPVRGTRV
                     VPTSCDLCFWKCNALAHVRDGRLWKITGNPDDPMSRGRLCPRGTGGIGAHFDPDRLRA
                     PLMRRKARGSESWVEVTWDEALGHVADRMQKIKAEHGPESMALFAHGIGGTFLKHTLK
                     AYGGVNIAAPSFAQCRGPRDVGFELTFGEGIGSPERTDIRSARCLVLIGSHLGENMHN
                     TQVQEFAEAIANGATIIVADPRFSIAASKAKHYLPVKPGTDLALLLAWMNVLVTEDLY
                     DRAYVAQFGFGFEQFAAEIGQRTPEWAYPETGIPPDLIRETAREMARHRPATLVHPGR
                     HTTWYGDDTQRGRAIALLNALLGSWGRRGGFYYPSSMDVPSYPYPPYPTSTRGKVDNP
                     DNKYPFASEAITSGIRDATISGKPYPIKGWMVYATNLLQALPRQEETIQAIQNLDLMV
                     VVDVLPSEVAGWADVVLPESVYLERYDELNVELFRDPFVALRQPVVAAPGDQKPNWWI
                     ARQLAIKLGLGNYYPWTDIEEYLDHRLKGAGHSLAELRQRGVVRGAQRPLYFEDGVAP
                     EFATPSGKIEFHSTQLADAGFDPVPRYRRPAEGPAGSFRLLYGRAPVHTFSRTQTNPV
                     LQDAMRENEVWVNASVGARLGLRSGDHVTLRNQDGVVSNRVKARVTEAIRPDCVYMVH
                     GFGHTSRALRGAYLKGASDAQLITRYATDPLMGGTGTNVNFVTIEPGA"
BASE COUNT         1694 a         3457 c         3559 g         1627 t
ORIGIN      
        1 tgccgccggg cggcacgctg gaagtgctcg ccaccgaccc gggcgcggtc aaggatttcg
       61 aggccttctc gcgcaccacg ggccacgagc tcgtcgagtc gtccagcgaa gggaacgtgt
      121 tccggttcgt cctgcggcgc aaggcctgat gcggccggcc gggctggcgg cgttgtcgca
      181 gcgcagcggc gaatgcccgc ggctgcgcgt agcggtaagt cccggtgtcg cggccagggg
      241 acgggatcga aaataagcaa tctctaattt gtcatccgcg gccgccgcgc ggccatattc
      301 gggggtactc atgagcaggt tcagcactcg cacggtcctc ggcctcggcg tcgcgatggc
      361 gctcgccgcc ggatcggcct cggccaccaa cggctacttc acccacggcg tgggtaccaa
      421 gagcaaggcg ctcgcagggg ccggaagcgc caacccggaa gagatccttt ccatcgccac
      481 caacccggcc ggcctggcgc tgctgccgga gacggtggac gcgggcctcg gtttcttcag
      541 tccgtcgcgc cagtacaaga cctccgaaag catgttgaat ggcaactgct tcattcccga
      601 gggatcggag acccccttct gcccgttcac catcggcccg aacaacctgc agagtgggag
      661 cgagttgttc ctcgtgccat acgtggccat gaactggaag ctcgacgagc agagtgcgct
      721 ggccgtggcg ttctatgggc gcggcggcat gaataccaac tggcagggcg gcacggcgac
      781 atacgatccg gacggctact gcatcccgga cgtgcaaggc tgtgcctacg gccccatgac
      841 gttccacggc acctacggca gcggcagcct ggtcactccc tacggccacc cggcgcaggc
      901 cggcgacgcg ggcgtggacc tgatgcaggc attcctcaac accacctatg cgtggaagag
      961 cgccgatgac cagttcgcag tcggcgtatc ggcgatattc gccatgcaac tgttccaggc
     1021 gcgcggcgtc ggggcgttcg cgccgtacac gaagacgtac atcgatgcgt acatgcagac
     1081 cgggatgccg gcaatgccga agaacctgtc gggcaatggc cacgacagtt cctacggatt
     1141 cggcgggtcg gttggcggca tctggaacat caacaagatg ttcagcgtcg cggccgcgta
     1201 tacgtccaag atgttcatgt cgaagttgag cgactactcg gatctcttcg ccgagggcgg
     1261 cagcttcgac atcccgtcga cgatgacgct gggcgtgacg gtgcggccga ccgagcagtg
     1321 ggcagcgtcg ttcgactacc agacgatctg gtactccgac acgccgtcgg tcagcaaccc
     1381 gatccagaag ctcttcgact gtccgacggt cggcgggtcc aagccccaga actgcctcgg
     1441 cggcaacaac ggtccgggct tcggctggaa ggacatggac atctacaagg tcggcctcga
     1501 gtggaggtac gacgacgcct ggacctatcg cctcggcttc agcgtcccgg attcgcagcc
     1561 gatcccgaag aaccagatgg aattcaacat cctggcgccg ggtgtcgtcg agcagcacta
     1621 caccgctggc ttcacgcaca cgctggaggg cggcgaccag ttcagcgttg cggtgatgta
     1681 cgccccgacg gtgtcgcaaa agggcgtgaa caacttcgat ccgacgcaga ccatccagtt
     1741 ctcgatgcac gagttcgagc tcgaggttgc gtacagttgg aagcgctgac gatcgatcgt
     1801 gacggggacc gggtgaccta gtggggggta acgcgaggcg gccaggacgg ccgcctcgcg
     1861 cagccgccgg ggcgggacca gaaacatgca ggtgattccc gggctcgtgc tcgcattcgt
     1921 cctcgcggtg ctcggcaatt accttgccga cgccatcgcc gtcgcgttgg gactcggtcc
     1981 cggcgccatc agcgggatca tggtggcgat cctgctcgga ctcgcgatgg gcaacctgtt
     2041 ccgcctgccg gcgtcgctca agccgggcat cgacgtcgcc gtgaagcgcg tgctgcggat
     2101 cggcatcgtc ctgctcggcc tcaagctcag catcgtcgag gtcggcgcga tcggcctgaa
     2161 gtcgctgccc gtgatcctgg tgacgatccc gacggcgata ttgctggtga ccttcctcgg
     2221 ccggcgcctc ggcctgcccg atcgcctggg gacgctgatc gccgtcggca ccggtatctg
     2281 cggcaatacc gcgatcgtcg cggtgtcccc gaccatcgcg gccaaggagg aggagaccag
     2341 ctacgcggtg gcctgcatca cggtcttcgg cctgttcgcg atgctcgcct atccgttcgt
     2401 ggcccactgg ctgttcgacg gcgacccgtt tcgcgccggg ctgttcctgg gttcgtcggt
     2461 gcacgacacc tcgcaggtcg ccggggcggg gatggtgtac caggactact accacgaccc
     2521 ccaggcgctg aatgtcgcca cggtcacgaa gctcgagcgc aatctctcga tgctcatcgt
     2581 gatcccgctg atgtccatcc tgtaccaccg gcgctcgtcg gagggaacgg agccaccgcc
     2641 gtggtggacg atggtgccga tgttcgtcgt cggcttcgcc gcgatgagtg cgctgcgtac
     2701 cgtcggcgac ctcggctcgc gccccttcgg gctgctcacg gcggaccagt ggcacgaggt
     2761 cgtgaagcag gcgtcgcacg tcacgtccta ctgcctcgcg atcgcgatgg ccggcgtggg
     2821 gctggggacg cgcatcggca acctgcgctc catcggcctg aagccgctgg gactcgggct
     2881 cgtgtccgcg ctcatcgtcg gcttggtgag cactctgctg atccggacgc tgtactgaat
     2941 ttgcggtatg tttgacgggc agccggccgg gctgcccggc tgcacggggg agagcaccat
     3001 ggaccgacgg tcattcgtgc gcggcgtggc gagtgcaacg gcgctggccg gcggacagtt
     3061 gctgctgccg cggtcgctgc tggccgacgg cctgccgtcg ggcgcgctcg aaagcgagca
     3121 gatggtcgcg ctgccgggca agcagccgct catcaagcgt tcctttcgcc cgcccaactt
     3181 cgagacgccg gtcggcatgt tctccgacgt catcacgccg aacgaccgct tcttcgtgcg
     3241 ctggcacctc gcgtccatcc ccgagatcga tccagcggcg tggcgactca gtatcggcgg
     3301 cgacgcggcg tccgcaccgt acgagctgac gcttgcgcaa ctgcagaacg acttcgagca
     3361 ggtcgaggtc gtggccgtct gccagtgttc cggcaatcgg cgcggcctgt ccgatccgca
     3421 cgtgcccggc gtgcaatggg gctacggggc gatgggcaac gcgcgctgga agggcgtgcg
     3481 cctcaaggac ctgctcgcga aggcgggtat caccaaggat gccgtcgagg ttgctttcga
     3541 cggcgcggac cgcggggcgc tcgacgcgac cccggacttc gtgaagagca ttccggcgtg
     3601 gaaggccatg gacgggaatg cgatcgtcgc gtgggaaatg aacggcgggc cgctgccgca
     3661 ctggaacggc gccccggcgc gcgtcgtcgt gcccggctgg accgcgacct actggatgaa
     3721 gaaggtggtg gccatccgcg cggtctccca gcccgtcgcg agcttctgga tggcgaaggc
     3781 gtaccgcata cccaagggca agttccccac ggtcgatcgg tttctcacgc aggagacgcc
     3841 cgagaacacg ccgatcacgg agatcgtcgt caattcgctc atcaccaacc tgaccgacgg
     3901 ccagaaggtg agggccgaca cgctggtgac ggtcaagggc atcgcctggg acgctggcta
     3961 cgggatccgc tccgtggacg tgtcggtgga cggtggtctc ggatggcact ccgcgcagct
     4021 cgggacggat atcggccgct actcgttccg cgagtggcaa tacgtcttca aggcgaggaa
     4081 gggcgagcac gtcgtgtcgg cacgcgcgtc gaacggcgtg ggcgagacgc aggttgatca
     4141 actcattttc aacgccgctg gatacaacaa caacgtgatc cagcgcatcg gcatcaaggc
     4201 cgtctgagga ggagcaccat gaaggcgacg cacttgctcg tcctgctcgt cggctgggcc
     4261 gctgccgacg tggcgctcgc cgacgagtcg cagatccgca tgaaggaagc gcccgagacc
     4321 ccgacgctgg tggcgaactg cagcggttgc cacagcctcg actacgtgca gatgaactcg
     4381 aagttcatga agcgcgcgac ctgggaggcc gaggtgcaca agatggtcgc cgtgatgggc
     4441 gcgcccgtga gcgaggcgga ccaggcggcg ctcgtcgagt acctgacgcg cgagtacggc
     4501 gtcgcggact gagcggcacc gcctgccagg cggcccgggt tcagcagcct tccttcttct
     4561 tcctcggcgt catcggcgcc gccggccggc ccgtgcctgc cgcccgcgca gggaatgagg
     4621 cctcggccgc tggcggtggc atggcctgtg gcaggccggg ctcggcctcg ggcgccgttc
     4681 cggccgagcg aggctgtccg ccgaagaacc gcgccaccgc cgccgtcctc tcgaaacggg
     4741 cccggtcgag cgggctcgcg tccgcgggca tgaccgggaa cagcacgtcg tccttccagc
     4801 cgtcgaggcc gagcagcacc gtgtacacat tggtgtagcc gcgggcgcgc atgagcatcc
     4861 aggcctgggc ggcatgaatt ccgccctccg aatagaggac gatcttttcg gtcggctgcg
     4921 gcacgaagtc cgcgatcgtg gccagcggga cgttctccgc agtcgggatg tggtaccggg
     4981 cgaactcgtg ctcgttgcgc aggtcgagca gccggtagtc ggcgcgtccc tcgatgatcc
     5041 acccggcgag ctccgtgggc gtcacgtggt cgacttcctt cgcgacgatc gatgccagct
     5101 cgcgcgcgtc cagtttcacg aacgggccgc gatgcggctg gctggcgagc gcgaccgcac
     5161 cgagcgccac cgcgatggcg ccgagcttct tgttcagcgt gaggtcggac catctcatgc
     5221 gcggtccctc gcccagcgct cgaccttggt cgcgccccag aagccgccga ccgccatgag
     5281 gacgacggcg aacacgatca cgccccaggg caggccgagg acttcgggca gcgtgacctg
     5341 ccccatctcg tcggcgttca cgaagccctt gatcgacggg tacacctcgc cgtacacgaa
     5401 ggtcccggcg aagatgccga gtgcgtacag gacgccgtcg atccgcccgg tggccaccgc
     5461 cgccgccgac gtgcccgggc agtagccgcc cacgacgaac ccgactccga gcacgaggcc
     5521 gccgacaatc tgcggccaga ggtaggtcgg caccaggtag acctgcgaga ggtccagcca
     5581 gccgagccag ccgaggtagg tgacgccgag cagcgccgtg acgatcgcgg tgaacatcac
     5641 cttgaacacc gacatgtcga acaggtagaa ctgcgccgcg agcttgcgcg cgttgccgaa
     5701 cccggcgcgc tccaggcaga aaccgaagcc gatgccgatc agcaccgcga cgatgagcga
     5761 cagctcgtcg ccgaagtaac cgtacttgta gagtggtgtc atcatcgcca ttgccaccgc
     5821 atgaaccagg cgaccgcgta gccgccgacg aagaccatga tcatgaaggc ccagctgccg
     5881 acgttgagca gtgccccgcc cgtcagcgcc tggccactgg tgcagccgcg cgcgaggtgc
     5941 gcgccaaacg ccatcagcat gccgccggcg aatgcgcacg cgagccgcac gcccgtcgag
     6001 acccgcggcc ccttctcgac cgtggcgtgc acccgccccg cgagcagtcc ggagagcagt
     6061 gcgcccgcca tgacgccgat gacttcgaat acgagccacg ccttgagcgg gtgcgtggtg
     6121 ccgtcaccga ggtagccctg gaagtattcg ttggcctgcg cgtgcgccgg cgccaccagg
     6181 ttcgtgagcc aggccacggt cgagctgaac gcgcccgacg cgccgagccc gcgccccatg
     6241 acgacgaacg cggcgagcag cacgaggccg aggccgatcc cggccaggta cgggttcgcg
     6301 tagccgctgc cgcggtcgct ctcgaccccg ttccgggtcg tgtccttggg gactgaattc
     6361 acgatgctta cctccacgtt ggcgaacggc gccgcgccta tggcgaaatc cagtggctgt
     6421 attggcctgc cgcaacgatg acgtagcgca gcgcgaagcc gccggccatc accagcagcg
     6481 gggcgatgac cgtgtggcgg atgcggtgcg agaccatcaa cgactggatc gcgagtggca
     6541 gcaagatacc gagcccgacc acgaagaccc agaaggccgc cgtgtagggt cccccgagca
     6601 gcagcgcgac ggccgtggcg tgctgcgcgc cggacgtcag gtgcccgatc aggaacagcg
     6661 cgatcagcgc cagttcgacg gtcatgaaca gcacgtcggc acgcaggagc gtcgcccgct
     6721 cggtcgcgtc gcgcgcgacc atgtgagcga aggcggcacc ggtcgacaga cccgacacga
     6781 ggaacagcgg tcccaggacc gcgctcgccc acaggggccg cgcaccgagg ctcgagagca
     6841 ggatgccggt gtagatgccc agcgccgagc cggccacgat gttcaccgtg ccgagccgcc
     6901 gcaggcggtc ggcgtcgagt tgccacctgc gcagcgatgc gagtgcaggc caccgcgaca
     6961 gcagcccttc gggcacgccg gccagggcac cgagcgccag gaccgggtag accagcagca
     7021 ggatccatcc accccacgac atcggcgagg tcggctgcag cgtgaggtag aggcgccaca
     7081 cgtacaggcg gtgctcgagg tcgaggaata gcgcgcccat gcccaggctc aacagcacga
     7141 taccggccca cggcagcacg taggcgaccg tccgtccggc cggcgtgcgc ccctggcgca
     7201 tgaaccagcc ggacaggacc atgcagccgg ccacccagcc gccgaggaac aggtacacgg
     7261 gaatctgcca gccccacacg tgcagcgtgg ggtccaccag cgggttgtgc cgcgtcgtga
     7321 cgagctcgag catcacgtca ctccaggtag tggatgcgcg gcttcgtgcc ggcgtcgggc
     7381 ttcagcacga agtggcgacg cgacgcgatc tgccggctca cttcggagtc gggatcgtcg
     7441 aggtcgccga acgtcatgct gtgcgtggga cagaccgaca cgcaggccgg atccaggccc
     7501 tgctcgaccc ggtggatgca gaacgtgcac ttgtcggcgt agccgtccgg gtggatgaat
     7561 cgcgcgtcgt acgggcacgc ggcgaggcag gccttgcagc cgatgcactt gccgtgatcg
     7621 accagcacga ccttgccgta gtcgtgcacg tggctcgcgc ccgtcgggca gcaactgacg
     7681 cacggcggct cgtcgcagtg attgcagcgc tcggacgtga tggtgagccg cagctcgggc
     7741 atcgttccct gcaggcgttg cacgatccag tctcggcagt gcccgtcggg gacgtcgttc
     7801 tcggtcttgc aggcgacgac gcagtccatg cagccgacgc acttcctcgt gtcgatgacc
     7861 atgccgaatc gcgccatggc tcaggctccc ggttcgatcg tcacgaagtt gacgttggtg
     7921 ccggtgccgc ccatgagcgg gtcggtcgcg tagcgggtga tcagttgcgc atcgctggcg
     7981 cccttcaggt aggcaccgcg cagcgcccgc gaggtgtgcc cgaacccgtg caccatgtac
     8041 acgcagtccg ggcggatcgc ctcggtgacc cgcgctttca cccggttgct cacgacgccg
     8101 tcctggttgc gcagcgtcac gtggtcgcca ctgcgtagcc ccaagcgcgc cccgacggac
     8161 gcgttcaccc agacttcgtt ctccctcatt gcgtcctgca ggaccgggtt ggtctgcgtg
     8221 cggctgaacg tgtgcaccgg cgcgcggcca tacaggaggc ggaaggaacc agcgggccct
     8281 tcggccggtc gccggtaacg aggcaccgga tcgaaccctg cgtccgcgag ctgggtggaa
     8341 tggaactcga tcttgcccga cggcgtggcg aactccggcg cgacgccatc ctcgaaatag
     8401 agcggccgct gcgcgccacg caccacgccg cgctgcctga gttcggcgag actgtggccc
     8461 gcacccttca gccgatgatc gaggtattcc tcgatgtccg tccacgggta gtagttgccg
     8521 aggccgagct tgatcgccag ctggcgggcg atccaccagt tcggcttctg gtcgccggga
     8581 gccgcgacca cgggctggcg cagcgccacg aacgggtcgc ggaacagctc gacgttcaat
     8641 tcgtcgtagc gttccaggta gacggactct ggcagcacga cgtcggccca gcccgcgacc
     8701 tccgacggca gcacgtccac gaccaccatg agatcgaggt tctggatcgc ctggatggtc
     8761 tcctcctgcc gcggcaacgc ctgcagcaag ttggtggcgt agaccatcca gcccttgatc
     8821 gggtagggct tgccggagat cgtggcgtcg cggatgcccg aggtgatggc ctcgctcgcg
     8881 aacgggtact tgttgtcggg attgtccacc ttgccacgcg tcgacgtcgg gtagggcgga
     8941 tacgggtagg acggcacgtc catgctggac gggtagtaga agccgcccct gcgtccccag
     9001 ctgccgagca gcgcgttcag cagcgcgatc gcgcggccgc gctgcgtgtc gtcgccgtac
     9061 cacgtcgtgt gccggccggg gtgcacgagc gtcgccggac gatggcgcgc catctcgcgc
     9121 gccgtctcgc ggatcaggtc gggcgggatc ccggtctcgg ggtaggccca ctccggggtg
     9181 cgctgcccga tctccgctgc aaactgctcg aacccgaagc cgaactgcgc gacgtaggcc
     9241 cggtcgtaga ggtcctccgt caccagcacg ttcatccacg cgagcaacag cgccaggtcc
     9301 gtgccgggct tcacgggcag gtagtgcttc gccttgctcg ccgcgatcga gaagcgcgga
     9361 tcggccacga tgatggtggc gccgttcgcg atcgcctcgg cgaattcctg cacctgggtg
     9421 ttgtgcatgt tctcgccgag gtgcgagccg atcagcacca ggcagcgggc actgcggatg
     9481 tcggtgcgtt ccggcgagcc gatgccttcc ccgaaggtga gctcgaagcc gacgtcgcgc
     9541 ggtcctcggc actgtgcgaa cgacggtgcg gcgatgttga cgccgccgta ggccttcagg
     9601 gtgtgcttga ggaaggtgcc gccgatgccg tgggcgaaca gggccatcga ttccggcccg
     9661 tgctccgcct tgatcttctg catccggtcg gcgacgtgac cgagcgcttc gtcccacgtc
     9721 acctcgaccc aggactcgct gccgcgggcc ttgcgtcgca tgagcggggc acgcaaccgg
     9781 tccgggtcga aatgggcgcc gatgccgccg gtgccgcgcg ggcacaggcg tccgcgcgac
     9841 atcgggtcgt cggggttgcc ggtgatcttc cagagccgac cgtcgcggac atgggccagc
     9901 gcgttgcact tccagaagca gaggtcgcac gaggtgggaa cgacgcgcgt cccgcggacc
     9961 ggaccgtcgc gcgtcacggc ctggagcgtg cgcgccccgg cggctgcaag ggccgtcgcc
    10021 ccggctccca ggccggcgat cttgaggaac ctgcggcggg aaatgtggct catggacgcg
    10081 tggagcgact ctgacgcatt agagatgtct aatatggtac cgggccccgg gtgcggcagg
    10141 gtatgtacaa gccgcagggc cgggccgacg cccgattgat cgccggatcc gggtcgttca
    10201 tcctctggcg cccggctcga ggagactgga cgagaccgtg cggctgctgc tggcagaaga
    10261 cgacccgatg atcggcgagg cagtccgtgc cgggctgcac cagcagggct ttgccgtcga
    10321 ctgggtccgc gacggcg
//
LOCUS       DAOMFD010000063        10272 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1836_length_10272_cov_34.782617, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000063 DAOMFD010000000
VERSION     DAOMFD010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10272)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10272
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1836_length_10272_cov_34.782617"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PL152_05415"
     CDS             <1..608
                     /locus_tag="PL152_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQR48751.1"
                     /translation="IDESEAVRHQAAEETAAALAAALPDLRVGLVHGRMPTPRKDAAM
                     GAFKRGEIDLLVATTVIEVGVDVPNASLMVIENAERMGLAQLHQLRGRVGRGSVESSC
                     VLLYQPPLPPLAHERLAVLRDTNDGFEVARRDLELRGPGELLGTRQTGLMQLRVADLV
                     RDADLLPGVQKAATLMLAGPRSNMEALIRRWVGQGERFGKV"
     gene            626..1540
                     /gene="ubiA"
                     /locus_tag="PL152_05420"
     CDS             626..1540
                     /gene="ubiA"
                     /locus_tag="PL152_05420"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HQR48752.1"
                     /translation="MGNATDIDQDRAGATGSPAFRRLREYARLMRIDRPIGIWLLLWP
                     VLWALWISGGGHPDERIFVIFLAGTFVMRSAGCVMNDFADREFDPHVRRTADRPLAKQ
                     SVSPAEALGLFAVLGLVALALVIPLNRPTQVLALIGGVLAVTYPFLKRFFALPQAYLG
                     LAFSWSVPMAFAAQTGGLPPIAWVLFLAGVLWTTAYDSIYAMVDREDDLVIGIRSSAI
                     LFGRADRGIIALLQAGALGGLGLAGYMGGLGRWYWCGLAVAAGSVIHQQWLIRGRDPE
                     RCFRAFLNNNLFGFAVFAGILLHYLFAG"
     gene            complement(1550..2767)
                     /gene="nhaA"
                     /locus_tag="PL152_05425"
     CDS             complement(1550..2767)
                     /gene="nhaA"
                     /locus_tag="PL152_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HQR48753.1"
                     /translation="MTQRQQGSHRPMDAVREFLKLESASGILLVVAGALAIVAANTPM
                     ADWYEQLLATHFSVRFGELALDKPLVVWVNDLLMAIFFLLVGLEIKREVVTGELADAG
                     KVALPAVAALGGMIVPAAIYAAINWNDPVGIRGFAIPSATDIAFALGVLSLFGRRVPV
                     GLKIFLMTLAVLDDLGAIVIIALFYTSELSVTALLYAGLALVALAVLNRFRVTRIAPY
                     ILAGAALWLCVLKSGVHATLAGVLTAFFVPARDERDPEHPPLRKLEHSLHPWVAYGIL
                     PVFAFSNAGVNLEGLGLSDLLAPVPLGILLGLFVGKQVGVFGFAWVAVRVGIARMPAG
                     VDFRQLYGAAVLCGIGFTMSLFIGMLAFENAGGGEVVLTDRLGIIVGSLLSAVVGYVV
                     LHFVLPRPSSAEA"
     gene            complement(2764..3444)
                     /locus_tag="PL152_05430"
     CDS             complement(2764..3444)
                     /locus_tag="PL152_05430"
                     /inference="COORDINATES: protein motif:HMM:NF012383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HQR48754.1"
                     /translation="MLSGLALPPRCVLCGRRGQGPCLDLCMHCEATLPEPMHALQVDA
                     PPLARRYAAFTYAFPVDRLVQSLKYRGELAVGRVLGSLLGHRAATLGLHLDVDCIVPV
                     PLHPLRHAERGYNQAAEIATWAGRAVRRRVIAGAVRRVRATPPQVGLRLHERSLNLAG
                     AFVADAQLGNRRVVVVDDVLTTGSTVAAVATALREAGAVSVDAWCVARADLPGQVHWP
                     LEAEAQAT"
     gene            3595..4572
                     /gene="bioB"
                     /locus_tag="PL152_05435"
                     /pseudo
     CDS             3595..4572
                     /gene="bioB"
                     /locus_tag="PL152_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009315099.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004076 - biotin synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
     gene            4602..5786
                     /gene="bioF"
                     /locus_tag="PL152_05440"
     CDS             4602..5786
                     /gene="bioF"
                     /locus_tag="PL152_05440"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008710 - 8-amino-7-oxononanoate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HQR48755.1"
                     /translation="MRALESELAAGLAAQRAAHRARTRTIVESRAVDATRIRVEGREV
                     VAFCSNDYLGLAGHPLVTAAFVEAARRWGVGSGASHLVSGHTLEHHALEDELAQFVGR
                     PRAVLFSTGYMANLAVVRALARRGDSVLEDRLNHASLIDAGLASGARFRRYAHSDVAA
                     LRTRLTRAGSARTLVATDGVFSMDGDVAPLRELAAACREHGAALFVDDAHGFGVLGDG
                     GRGSVERAGLALDDVPALVCTLGKAVGTFGAFVSGSEALVESLVQSAREYIYTTALPP
                     AVAAATRTALRVIAAEPGRRARVIALARAFGAEAKRLGLSLTASDSPIRPVLLGSESA
                     ALEASRALLDAGLFVPAIRPPTVPAGTSRLRVTFSAAHADADVDRLLDALAALRRRGL
                     LH"
     gene            5783..6583
                     /gene="bioH"
                     /locus_tag="PL152_05445"
     CDS             5783..6583
                     /gene="bioH"
                     /locus_tag="PL152_05445"
                     /EC_number="3.1.1.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR01738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HQR48756.1"
                     /translation="MTPHVEIRGRGPDVVLLHGWALHGGMWGAWLDQLAQHARLHLVD
                     LPGHGHSAWSEDIRGLAGFARAVRPVVPQGAVLLGWSLGGMIALELARSEPALAAALV
                     LVATTPRFAAGDGWEHGMPASVLDEFATGLAADHRGTVQNFLALQTRGDQNALDALRQ
                     LRRSLEAHGPPDRRALDAALQVLRTADLRDALPRMAAPALVIAGDHDRLTPPQAGRAI
                     AEALPHARFLQIPRSGHAPFLSHGPEVLREVLGFVARHGAAATLEETA"
     gene            6580..7485
                     /gene="bioC"
                     /locus_tag="PL152_05450"
     CDS             6580..7485
                     /gene="bioC"
                     /locus_tag="PL152_05450"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: protein motif:HMM:TIGR02072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HQR48757.1"
                     /translation="MNRAPPDASEFEIDRREVRRSFGRAAGSYDAAADLQRRIRAELL
                     ERLDVVRMTPVAVLDLGTGTGHAAIALKRRYPRCRVVALDLAESMLREAGRRQTLLRR
                     FRRVCADAAGLPFRDGSFDLVFSNLMLQWCNDPDRVLAECRRVLRPGGLLTFSTFGPD
                     TLIELRRAWSAADDRVHVSRFLDMHDVGDALVRAGLAEPVMDVERMTLDYGDVRQLMR
                     DLKAIGAHNANAGRPRGLTGKRAYARMVAAYETSRRDGRLPATYEVVFGHAWAPVGQP
                     RRHGVAAGEVRIPVSRIGRTRGGSS"
     gene            7583..8302
                     /gene="bioD"
                     /locus_tag="PL152_05455"
     CDS             7583..8302
                     /gene="bioD"
                     /locus_tag="PL152_05455"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF024891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004141 - dethiobiotin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HQR48758.1"
                     /translation="MVSEVPVSRGIFITGTDTGVGKTVVAAALVRLLAHHGHRVVGLK
                     PIASGATRTADGLRNADALALAAESSEPLPYRQTNPYCFEPAIAPHLAAREAGTSLSL
                     DALSDWYADVSSVADVAVVEGAGGWRVPLHPEGFLSDLPERLGLGVIVVVGLRLGCLN
                     HARLTLEAIAATGRCPFLGWIGNQVDPDFQRVEENLRSLERLLGQAPLAVVPWLAAPS
                     AASAMEWLDGPRLVPTVFPPQ"
     gene            8550..9425
                     /gene="coxB"
                     /locus_tag="PL152_05460"
     CDS             8550..9425
                     /gene="coxB"
                     /locus_tag="PL152_05460"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HQR48759.1"
                     /translation="MKTVKRALAACAAFLAGSGPAMAAWSDLNLRVGVTPLSRDIHGL
                     HMLILWICVAIAVAVFGVMIYSIATFRKSKGAVPATFDHSTKAEIVWTVIPVLILVGM
                     AIPAAKTLVRIDDSSGADMTIKVTGYQWKWQYEYVGQGVSYYSTLAQASNFARELRSG
                     VDPYSVQNYLLDVDHPLVLPQGVKVRVLVTSADVIHSWWVPDFAIKKDAIPGYINELW
                     ILADQAGTFRGQCVELCGRDHGFMPIVVEVKPRAEFDAWLAEQKAATQAANAPATQVA
                     LNHVEPAAVRVAAAE"
     gene            9432..>10272
                     /locus_tag="PL152_05465"
     CDS             9432..>10272
                     /locus_tag="PL152_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQR48760.1"
                     /translation="MSATATHVAGAAHDHHDDHGPAKGIMRWLTTTNHKDIGTMYLVF
                     SLLMFLLGGAMALVIRAELFMPGMQIVDPGTFNTLTTVHALIMVFGAVMPATVGLANW
                     MIPMQIGAPDMALPRMNNFSFWLLVAAFSLLILSLFMPGGGPAAGWTLYPPLVLQTGD
                     ALPLAIFAIHLAGASSIMGSINIIVTIMNLRAPGMTLLKMPLFVWTWLITAYLLIAVM
                     PVFAGAVTMLLTDHFFHTTFFAAAGGGDPVLYQHVFWFFGHPEVYIMILPAFGIVSEI
                     IPTF"
BASE COUNT         1478 a         3717 c         3523 g         1554 t
ORIGIN      
        1 tgatcgacga gtccgaggcc gtgcgtcacc aggccgcgga ggaaaccgcg gcggcgctgg
       61 cagcggccct gccggacctg cgcgtcggac tcgtgcacgg ccgcatgccc acgccgcgca
      121 aggacgccgc gatgggcgcg ttcaagcgcg gcgagatcga cctgctcgtc gccaccaccg
      181 tcatcgaggt cggcgtggac gtgccgaacg ccagcctcat ggtgatcgag aatgccgagc
      241 gcatgggtct cgcccagctg caccagctgc gcggccgcgt cggccggggc tcggtcgaga
      301 gcagttgcgt gctgctctac cagccgccat tgccgccgct cgcccacgaa cgactcgcgg
      361 tcctgcgcga caccaacgac ggattcgaag tcgcgcgccg cgacctcgag ctgcgcgggc
      421 cgggtgaact gctcggcacc cgccagaccg ggctcatgca gctcagggtc gccgacctcg
      481 ttcgcgacgc cgacctgctg ccgggggtgc agaaggccgc gacgttgatg ctggccgggc
      541 cgaggtccaa catggaggcc ctgatcaggc gctgggtggg gcagggcgag cgcttcggca
      601 aggtctgagg cacccgcgca atccgatggg caacgcgacc gacatcgacc aggaccgcgc
      661 cggagcaacc ggctcgcccg ccttccggcg gctgcgcgag tacgcgcggc tcatgcgcat
      721 cgaccgcccc atcggcatct ggctgctgct gtggccggtg ctgtgggcgc tgtggatctc
      781 cgggggaggt cacccggacg aacgcatctt cgtgatcttc ctggccggca ccttcgtgat
      841 gcggtcggcc ggctgcgtca tgaacgactt cgcggaccgc gagttcgacc cgcacgtgcg
      901 acggacggcg gatcgtccgc tcgcgaaaca atccgtctcg cccgccgagg cgctcggcct
      961 cttcgcggtg ctcgggctcg tcgcgctggc gctcgtgatc ccgctcaacc gcccgacgca
     1021 ggtgcttgca ctgatcggcg gcgtgctcgc ggtgacgtac ccgttcctca agcgcttctt
     1081 cgccctgccc caggcgtacc tcgggctcgc gttcagctgg tcggtgccga tggcgttcgc
     1141 ggcgcagacc ggcggactcc ccccgatcgc ctgggtcctg ttcctggccg gcgtgctgtg
     1201 gacgaccgcg tacgacagca tctacgccat ggtggaccgc gaggacgacc tcgtcatcgg
     1261 catccgctcg tcggccattc tgttcggtcg cgccgatcgc ggcatcatcg ccctgctgca
     1321 ggccggcgcg ctcggcggcc tcgggctcgc cggctacatg ggcggcctcg gtcgctggta
     1381 ctggtgcggc ctcgcggtcg ccgcaggatc cgtcatccac cagcagtggc tgattcgcgg
     1441 tcgcgatccg gagcgctgct tccgcgcctt cctcaacaac aacctgttcg gcttcgcggt
     1501 gttcgcgggg atcctgctgc actacctgtt cgccggctga cgcccgggat cacgcctcgg
     1561 cgctgctcgg cctcggcagc acgaaatgca ggaccacgta gccgaccacc gcggacaaca
     1621 gtgatcccac gatgatgccg aggcggtccg tgaggaccac ctcgccgccg cccgcgttct
     1681 cgaaggcgag catgccgatg aacaggctca tcgtgaaccc gatgccgcag agcacggccg
     1741 cgccgtacaa ctgccggaaa tcgacgcccg ccggcattct cgcgatgccc acgcgcaccg
     1801 ccacccaggc gaacccaaag acgccgacct gcttgcccac gaacagcccg agcaggatgc
     1861 cgagcggcac gggcgcgagc aggtcgctga ggccgaggcc ctcgaggttc acgcccgcgt
     1921 tcgagaacgc gaagactggg aggatgccgt acgccaccca ggggtgcagc gagtgctcga
     1981 gcttccgcag tggcgggtgc tccggatcac gctcatcgcg ggccgggacg aagaacgcgg
     2041 tgagcacgcc ggccagcgtg gcgtgcaccc ccgacttcag cacgcacagc cacagcgcgg
     2101 ctcccgccag gatgtagggc gcgatgcgcg tcacgcggaa ccggttcagc acggcgagcg
     2161 cgacgagcgc gaggcctgcg tacagcagcg ccgtcaccga cagttcgctc gtgtagaaca
     2221 gcgcgatgat cacgatggcg ccgaggtcgt cgagcaccgc gagcgtcatg aggaagatct
     2281 tgaggcccac cgggacgcgc ctgccgaaca gcgacagcac cccgagcgcg aatgcgatgt
     2341 ccgtggccga cgggatcgcg aaccccctga tcccgaccgg gtcgttccag ttgatggccg
     2401 cgtagatcgc cgccgggacg atcatcccgc cgagcgcggc gacggccggc agggcgacct
     2461 tgcccgcgtc ggcgagctcg cccgtcacga cctcgcgctt gatctcgagg ccgacgagca
     2521 ggaagaagat cgccatcagg aggtcgttca cccacacgac cagcggcttg tcgagcgcca
     2581 gctcgccgaa ccgcacgctg aagtgcgtgg cgagcagctg ctcgtaccag tccgccatgg
     2641 gcgtgttggc cgcgacgatc gcgagcgcgc cggcgaccac cagcaggatg ccgctcgccg
     2701 actcgagctt gaggaattcg cgcaccgcgt ccatcggccg gtggctgcct tgctgccgtt
     2761 gcgtcatgtt gcttgcgcct ctgcttcgag cggccagtgt acctgtcccg ggaggtccgc
     2821 ccgagccacg caccaggcgt ccaccgacac ggcgccggcc tcacgaagcg cggttgcgac
     2881 ggcggccacg gtgctgccgg tcgtcagcac gtcgtccacg acgacgaccc gccggttgcc
     2941 gagctgcgca tccgcgacga acgccccggc caggttgagg ctgcgctcgt gcagcctgag
     3001 tcccacctgc ggcggcgtcg cgcgcacgcg ccgcacggca cctgcgatca ctcgacgccg
     3061 caccgcacgg cccgcccacg tggcgatttc cgcggcctgg ttgtagccgc gctcggcgtg
     3121 acgcagcggg tgcagcggca ccggcacgat gcagtccacg tccaggtgca gtcccagcgt
     3181 cgcggcccga tgcccgagca ggctgccgag cacccgcccg acggcgagct cgccacggta
     3241 cttgagcgac tgcacgagcc ggtccaccgg aaacgcatag gtgaacgccg cgtagcgccg
     3301 ggctagcggt ggcgcgtcga cctgcagcgc gtgcatcggc tccggcagcg tcgcctcgca
     3361 gtgcatgcaa aggtccaggc aggggccctg gccgcggcgg ccacacagca cgcagcgcgg
     3421 cggcaacgcg aggccgctca acacgtccag ccacctgtca accgagtcca ggcgcattgg
     3481 ttgacagtcg ccggctctcc tctgaatact gccggccgac cgtccgcgcc cagcatcctc
     3541 cgcgtgccgc gcgggcggca gcacgaatat ctcccgcaaa ccgcaggaat cccgatgcca
     3601 accctggacc tggaccggct gggcgaagtg cgcaacgact ggacgctgga cgaggcgcgc
     3661 gccctgtttg cgctgccctt cagcgacctg ctctaccacg cgcagcgcgt gcatcgcctc
     3721 gccttcgacc cgaatcgcgt gcaggtgagc acgctgctgt cggtgaagac cggcgcctgt
     3781 cccgaggact gcgcgtactg cccgcagagc gtgcgctacg acaccggggt cgaacgcgag
     3841 gaggtcctcg acgtcgccgc cgtcgtggcc gcggccaggg ccgcgaaaca gggcggcgcg
     3901 acgcgcttct gcatgggtgc ggcctatcgc agccccaagc cgaagcagct cgagcaggtg
     3961 aaggacatgg tgcgcgccgt tcgcgcgctg ggactcgaga cctgcgcgac gctcggcatg
     4021 ctgtcgcccg gccaggctcg cgaactcaag gacgccggcc tcgattacta caatcacaac
     4081 ctcgacacgt ccgaggcctt ctacggcgag atcgtcacga cgcgcaccta ccaggaccgc
     4141 ctcgacacgc tgcagtcggt gcgcgacgcc gggctcaagg tctgctgcgg cggcatcctc
     4201 ggcatgggcg agtccgaact cgatcgcgtg cagctgctgc acacgctggc gacgctgccg
     4261 cagcacccgg aaagcgtgcc ggtcaaccag ctggtgcagg tgaagggcac gccgctcgac
     4321 ggcctgccgc cgctcgaccc gctggaactc gtgcgcacgg tcgcgacggc gcggttgctg
     4381 atgccgagct cctacattcg cctgtcggct ggccgcaccg acatgagcga cgccgtgcag
     4441 gcactgtgct tcctggccgg cgccaactcg atcttctacg gggaacggct gttgaccacg
     4501 ccgaacccgg gccgcagccg cgacgacgaa ctgttcgcgc gtctcggcat cgagcgggag
     4561 accgccgccg acgcgtccca cgtttcctga gcgcccggcc catgcgggcg ctcgagtcgg
     4621 aactggcggc cggtctcgcc gcgcaacggg ccgcccaccg ggcccggacg cgcaccatcg
     4681 tcgagtcgcg cgccgtcgat gccacgcgca tccgggtcga gggccgcgag gtcgtggcgt
     4741 tctgcagcaa cgactacctg ggactggcgg gtcatccgct ggtcacggca gccttcgtcg
     4801 aggccgcccg gcgctggggc gtgggcagcg gtgcctcgca cctcgtgagc ggtcacaccc
     4861 tcgagcacca tgcgctcgag gacgagctgg ctcaattcgt cggccggcca cgcgccgtgc
     4921 tgttctcgac cggatacatg gcgaacctgg cggtggtgcg cgcgctcgcg cgtcgcggcg
     4981 actcggtgct ggaggatcgc ctcaaccacg cgtcgctcat cgacgccggc ctcgcgagcg
     5041 gcgcccggtt ccgccgctac gcgcactcgg acgtcgcggc actgcgcacc cgtctcacac
     5101 gcgcgggatc ggcgcggaca ctggtcgcga ccgacggcgt attcagcatg gacggtgacg
     5161 tcgcgccgct gcgcgaactg gcggcagcgt gccgcgagca cggcgccgcg ctgttcgtgg
     5221 acgatgccca cggattcggc gtgctgggcg acggcggccg cggcagcgtc gagcgcgctg
     5281 gcctcgcgct cgacgacgtc cccgcgctgg tgtgcacgct cggcaaggcc gtgggcacct
     5341 tcggagcctt cgtttccggt tccgaggccc tggtcgagag cctcgtgcag tcggcccgcg
     5401 agtacatcta cacgactgcg ctgcctccgg ccgtcgccgc ggccacccgc accgcgctgc
     5461 gcgtgatcgc ggcagagccc gggcggcgtg cgcgcgtcat cgcgctcgct cgggcgttcg
     5521 gtgcggaagc gaagcgcctc ggcctgtcgc tcaccgcctc cgactcgccg atccggccgg
     5581 tcctgctggg cagtgagtcg gccgcgctcg aggccagccg ggcactgctc gacgccggcc
     5641 tgttcgtgcc ggcgatccgg ccgccgaccg tgcccgccgg cacctcgcgg ctgcgcgtga
     5701 cgttttccgc cgcgcacgcc gacgccgatg tcgaccgcct gctcgatgca ttggccgccc
     5761 tgcggcggcg cgggctgctg cattgacccc gcacgtcgaa attcgcgggc gcggcccgga
     5821 cgtcgtgctg ctgcacgggt gggcgctgca cggcggaatg tggggcgcgt ggctcgacca
     5881 gctcgcgcag cacgcgcggc tgcatctcgt cgacctcccg ggtcacggcc acagcgcctg
     5941 gtcggaggac atccgcgggc tcgctggctt cgcgcgggcc gtgcgcccgg tcgttccgca
     6001 gggtgccgtg ctgctcggct ggtcgctcgg cgggatgatc gcgctcgaac tcgcccgcag
     6061 cgagcccgcg ctggccgcgg cgctggtgct ggtggcgacg acgccgaggt tcgccgccgg
     6121 cgacggttgg gagcacggca tgcccgcttc ggtgctcgat gagttcgcga ctggactggc
     6181 ggcggatcac cgcggcacgg tgcagaactt cctcgcgctc cagacccgcg gcgaccagaa
     6241 cgccctcgat gcgctgcgcc agctccggcg gagcctcgaa gcccacggtc cgcccgaccg
     6301 tcgggcgctc gacgctgccc tccaggtcct gcgcactgcc gacctgcgcg acgcattgcc
     6361 gcgcatggcc gcgcccgcgc tcgtcatcgc cggcgatcac gaccggctga cgccgccgca
     6421 ggcgggccga gccattgcag aggcgttgcc gcacgcgcgg ttcctgcaga tcccgcgcag
     6481 cgggcatgct ccattcctgt cgcacgggcc cgaggtcttg cgggaggtcc tgggattcgt
     6541 cgcccgtcac ggcgccgcgg cgaccctcga ggaaacggcg tgaaccgcgc cccgccggac
     6601 gcgagcgagt tcgagatcga ccgacgcgag gtgcggcggt ccttcgggcg cgcggcaggc
     6661 tcctacgatg cggcggcgga cctccagcgg cgcatccggg ccgagctcct cgaacggctc
     6721 gacgtggtgc ggatgacgcc ggtcgcggtg ctcgacctgg ggaccggcac gggtcacgcg
     6781 gccattgcgc tcaagcggcg atacccgcgt tgccgggtcg tcgcgctcga tctcgccgaa
     6841 tccatgctgc gcgaggccgg gcggcgccag acactcctgc ggcggttccg ccgggtctgc
     6901 gcggacgcgg ccggcctgcc gttccgcgac ggcagcttcg acctcgtctt cagcaacctg
     6961 atgctgcagt ggtgcaacga cccggaccgg gtccttgccg agtgccgccg cgtgctgcgg
     7021 cccgggggcc tgctgacgtt ctccaccttc ggccccgaca cgctgatcga gttgcgccgc
     7081 gcctggtccg ccgccgacga tcgggtgcac gtcagccggt tcctcgacat gcacgacgtc
     7141 ggtgatgccc tggtccgcgc cggcctggcc gaaccggtca tggacgtcga gcgcatgact
     7201 ctggactatg gagacgtgcg acagctcatg cgggacctga aggccatcgg ggcgcacaac
     7261 gccaatgctg gacggcccag gggcttgacg ggcaagcgtg cctacgcgcg catggtcgcg
     7321 gcctacgaga cgtcgcggcg cgacggtcgg ctgccggcga cgtacgaggt ggtgttcgga
     7381 cacgcctggg caccggtcgg ccagccgcga cggcacggcg tggccgccgg cgaggtgcgc
     7441 ataccggtgt cccggattgg caggacccgg ggcggatcca gctgacgccg cgggctgacg
     7501 gatcgcctct aactctgcaa attttcttgt tcatttggaa taaaatagta aattcactcg
     7561 cgtattcatg aacatttagt ttatggtctc tgaggtacct gtcagccgcg ggatcttcat
     7621 cacgggcacc gacaccgggg tcggaaagac cgtggtcgcg gcggcgctcg tgcggctgct
     7681 cgcccaccac ggccaccggg tcgtggggct gaagccgatc gccagcggcg cgacccgcac
     7741 cgccgacggg ctgcgcaatg cggacgcact cgcgctcgcg gccgagagct ccgagccgtt
     7801 gccgtaccgg cagaccaatc cgtactgctt cgagcccgcc atcgccccgc acctcgccgc
     7861 gcgggaggcc gggacgagcc tgtcactgga tgcgctcagc gactggtacg cggacgtgtc
     7921 gtccgtcgcc gacgtcgccg tcgtggaggg agccggcggc tggcgcgtgc cgctccatcc
     7981 ggagggattc ctgagcgacc tcccggaacg cctgggcctc ggcgtcatcg tcgtggtcgg
     8041 cctcaggctc ggctgcctca accacgcccg actcacgctg gaagcgatcg ccgcaaccgg
     8101 tcgctgcccg ttcctgggct ggatcggcaa ccaggtggac ccggatttcc aacgggtcga
     8161 ggaaaacctc cgctccctcg agcggctcct cggccaggcg ccgctcgcgg tcgtcccgtg
     8221 gctggccgcg ccgtccgccg cgtcggcaat ggagtggctg gacggacccc gactcgtgcc
     8281 gacggtattt cctccgcagt gaccgggggt taaactgcgc gaccctcgaa ggtcgccagt
     8341 cctgcggggc ccgcattcgg gccccgggac acgtgccggc gaggtcgcag gtttcagcgg
     8401 ggagcagcgc cgcatcgcgg accgcctcga tatccacgcg tgagaagtgc gagtgaatcc
     8461 ccgacccggg tgccccagag cctccggccg gggtctggcc cggtccgggg aggccgcggc
     8521 cgatcaatga cactggagtt tgcctgccca tgaagacggt gaagcgtgcg ctcgcggcct
     8581 gcgcggcctt cctggccggt agcggcccgg ccatggcggc ctggtccgac ctcaacctgc
     8641 gcgtcggggt gaccccgctc agccgcgaca tccacgggct gcacatgctg atcctctgga
     8701 tctgcgtggc catcgccgtc gcggtgttcg gcgtcatgat ctattcgatc gcgaccttcc
     8761 ggaagtcgaa gggcgcagtg ccggccacct tcgaccacag caccaaggcc gagatcgtct
     8821 ggacggtcat cccggtgctg atcctggtcg gcatggcgat cccggcggcg aagacgctgg
     8881 tcaggatcga cgattcgagc ggcgccgaca tgaccatcaa ggtcaccggc taccagtgga
     8941 agtggcagta cgagtacgtc ggccagggcg tttcctatta ctccacgctc gcccaggcga
     9001 gcaatttcgc ccgtgagctg cgttccggag tcgacccgta cagcgtgcag aactacctgc
     9061 tcgacgtgga ccacccgctg gtgctgccgc agggcgtgaa agtccgcgtg ctcgtgacct
     9121 ctgctgacgt gatccactcc tggtgggtgc ccgacttcgc catcaagaag gacgccatcc
     9181 cgggctacat caacgagctg tggatcctcg ccgaccaggc gggaaccttc cgcggccagt
     9241 gcgtcgagct gtgcggccgc gaccacggct tcatgccgat cgtggtcgag gtgaagccgc
     9301 gcgccgagtt cgacgcgtgg ctcgccgagc agaaggccgc cacccaggcc gccaacgccc
     9361 ccgcgaccca ggtcgcgctg aaccatgtcg agccggcggc cgtgcgtgtc gccgccgcgg
     9421 agtaagacac gatgagcgca acagccaccc acgtcgcggg cgccgcccac gatcaccacg
     9481 acgaccacgg ccccgcgaag ggcatcatgc gctggctcac gaccaccaat cacaaggaca
     9541 tcgggacgat gtacctggtg ttcagcctgc tcatgttcct gctcggcggc gcgatggcac
     9601 tggtcatccg ggccgagttg ttcatgcccg gcatgcagat cgtcgacccg ggcacgttca
     9661 acacgctgac cacggtgcac gcgctcatca tggtgttcgg cgcggtgatg ccggcgacgg
     9721 tcgggctcgc gaactggatg atccccatgc agatcggcgc gcccgacatg gcgctgccgc
     9781 gcatgaacaa cttcagcttc tggctgctgg tcgccgcgtt ctcgctgctg atcctgtcgc
     9841 tgttcatgcc cggcggcggc cctgccgccg gctggacgct ctacccgccc ctggtgctgc
     9901 agaccggcga cgccctgccg ctcgccatct tcgcgatcca cctcgcgggc gcatcgagca
     9961 tcatgggctc gatcaacatc atcgtgacca tcatgaacct gcgggcgccg ggcatgacgc
    10021 tgctcaagat gccgctgttc gtgtggacct ggctcatcac cgcctacctg ctgatcgccg
    10081 tgatgccggt gttcgccggc gcggtgacca tgctgctcac cgaccacttc ttccacacga
    10141 cgttctttgc cgcggcgggc ggcggcgacc cggtgctcta ccagcacgtg ttctggttct
    10201 tcgggcaccc cgaggtgtac atcatgatcc tgccggcgtt cgggatcgtg tcggagatca
    10261 tcccgacgtt ct
//
LOCUS       DAOMFD010000064        10262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1839_length_10262_cov_33.594200, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000064 DAOMFD010000000
VERSION     DAOMFD010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10262
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1839_length_10262_cov_33.594200"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..317
                     /locus_tag="PL152_05470"
     CDS             <1..317
                     /locus_tag="PL152_05470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQR48761.1"
                     /translation="MSGNPKAFRDLLRTMHHGGSVALLGIPPDDTAIDWNEVIFKGLV
                     IKGVYGREMFETWYKMAAMLQSGLDISPIITHRFPVADYREGFEIMRSGQSGKVILDW
                     AA"
     gene            314..1279
                     /locus_tag="PL152_05475"
     CDS             314..1279
                     /locus_tag="PL152_05475"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR48762.1"
                     /translation="MRRLARIVAWILGGLAVLIAGYTAVTWAPDRPVEELVARWAPPP
                     STFIDLQGMKVHVRDEGARDDPVPIVLLHGTSDSLHTWDGWVRALEGRRRVIRADMPG
                     FGLTGPSPANDYSIGAYVRFVEALLDHYGIEHCVLAGNSLGGWVAWETTLAQPRRVDA
                     LILVDAAGYRYESQSVPIGFRLARIPLLNRLVLVTLPRSLVESSVRNVFGDPDRVTRE
                     IVDRHYELALRAGNRAAIVQRFRQAKAGVDEARIPEIKVPTLILWGGRDRLIPAEYGG
                     LFERDIAGSKLVVFEDLGHIPQEEDPARTVAAVQDFLATLPPASP"
     gene            1327..2601
                     /locus_tag="PL152_05480"
     CDS             1327..2601
                     /locus_tag="PL152_05480"
                     /inference="COORDINATES: protein motif:HMM:NF002461.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006835 - dicarboxylic acid transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate/amino acid:cation symporter"
                     /protein_id="HQR48763.1"
                     /translation="MARIRLLRSLYLQVLVAVVIGAVIGFAWPATGEALKPLGDGFIK
                     LIKMMIGPIIFCTVVLGISGMEDLKKVGKTGGLALVYFEVVSTIALVIGLVIVNVVRP
                     GAGVNADPATLDASAVAQYASQAESHGVTQYILDLVPSTLVGAFASGEVLQVLLVSVL
                     FGFALHAAGGRQLAVYRLVEQLSAVLFRVVGFIMRLAPLGALGAMAFTIGKYGLDALV
                     SLGKLMACFYATCLLFIFGVLGGIARWNGFSVWRFIRYIKEELFIVLGTSSSESVLPR
                     MMTRMEDLGVERTVVGLVIPTGYSFNLDGTAIYLTMAAVFIAQATNTPLDLTQQLTLL
                     AVLLLTSKGAAGVTGSGFIVLAATLSAVGHVPVAGVALILGIDRFMSEARSLTNLIGN
                     GVATVVVGKWCGQVDESTLRARLAGAEEPSSS"
     gene            2681..3760
                     /locus_tag="PL152_05485"
     CDS             2681..3760
                     /locus_tag="PL152_05485"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HQR48764.1"
                     /translation="MPNLLEPLAFQRGPAMSNRLMLAPLTNTQSHDDGTLSDEEFRWL
                     TMRAEGGFGLTMTCASHVQAQGRGFPGQLGIWSDAHIPGLTRLAATIKRHGSLAMAQL
                     HHAGMRSPAELIGQQPVCPSDNSEKGARALTAAEVEQLVEDFVLAALRAERAGFDGVE
                     LHGAHGYVLAQFLSAGINHRGDRYGGSLENRMRILFDILHGIRTRCRPDFTVGVRLSP
                     ERFDLRLAEMAVVARELMASGLIDFLDLSLWDVFKEPEEPEFKGRSLMSYFTGLERGK
                     VRVGVAGRISSGPDARHCLDAGADFAIIGRAAILHHDFPQRVRTDPGFKAVGLPVTAD
                     YLASEGLSPPFIRYMGNWKGFVAAA"
     gene            3897..4811
                     /locus_tag="PL152_05490"
     CDS             3897..4811
                     /locus_tag="PL152_05490"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR48765.1"
                     /translation="MSGSNEREGGRASGDLPAGGNAVVIEAWNTVLYEKFCRFKHLLV
                     EGLSQHSDAALERCRYQPGARVLDVGCGFGDSTLRIADKVAPGGEAVGVDCAENFVRG
                     AADEAREAGIQNARFFAADVQSDDLQGPYDHAFARFGTMFFDMPGAAMRNIRKALKPG
                     GTFMQIVWRRREDNPWLYEAELRVKAIVPAVSHEETNQVHCGPGPFSMSGPDMVSSML
                     TAAGFERIAFERYDCDICIGRDLNDAVEFAMALGPAGEAMRLAGEAAERLKPEVIAAL
                     EAALAPYVRPDGVWAPSSTWFISATSPG"
     gene            complement(4824..6668)
                     /gene="sppA"
                     /locus_tag="PL152_05495"
     CDS             complement(4824..6668)
                     /gene="sppA"
                     /locus_tag="PL152_05495"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HQR48766.1"
                     /translation="MFRAIGKVLRWIWSGLDGLRKVLHLVLLLVLFSLVVGLFSAPIP
                     LVRDKSALVIDPHGPLVEQLTGDPVERALSKALRTEPKETLLRDVVDAIESAADDDRI
                     SSLYLDLGGLEGGGLAKLQEVAAAIDDFRASGKPVIAYGDYYEQNQYYLAAHADEIYL
                     DPMGAAYVDGYDNYDLFVKDALDKLSVDVNIFRVGEYKSAVEIFSRNDMSPASREESV
                     GWLGAAWNTYKSDVAAAREFEPDRLQSYADESAALLRKADGDLAKMALDAGLVTSLKG
                     RLDVEDRLAQVTGEDEDTHSYIGVDFDSYLANVDSEQALTSKSKQKVAVIVASGDIVP
                     GEELPGMIGADTLAGELRDAANDEEVKAVVLRIDSPGGSVFASDVIRREVEAVRKSGK
                     PVVASMSSLAASGGYYIAMAADKIVASPATLTGSIGIFFMMPTFQRSLERLGVHTDGV
                     GTTALAGEFRLDRPMSDLSKDLIQQTIERGYRDFVGDVAKARTMSYEAVDKVAQGRVW
                     AGSDAKKVGLVDQLGGYEDAIDVAAGLAKLGDDYDVEYYDDDVSIGEALGLRVRIAVA
                     RVVAPLLPKSALPVVPKALAPLLKEVQRLERLRDPGNTYAYCLGCAID"
     gene            complement(6722..7066)
                     /gene="rplS"
                     /locus_tag="PL152_05500"
     CDS             complement(6722..7066)
                     /gene="rplS"
                     /locus_tag="PL152_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008220262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HQR48767.1"
                     /translation="MSNIIATLEKERMQREVPDFGPGDTVVVEVKVKEGDRERVQAYE
                     GVVIAKSNRGLNSSFTVRKISHGEGVERVFQTYSPSLGAITVKRRGHVRRAKLYFLRD
                     RTGKAARIEEKV"
     gene            complement(7063..7845)
                     /gene="trmD"
                     /locus_tag="PL152_05505"
     CDS             complement(7063..7845)
                     /gene="trmD"
                     /locus_tag="PL152_05505"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009683443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HQR48768.1"
                     /translation="MKIVVVTLFPEFVEQAVRFGVLGRALERGVLEVRTVNPRQFATD
                     VHKTVDDRPYGGGPGMVLKAEPLRLAMVEAERLVPEARRVYLAADGTPVTQRKVAELA
                     ALGELVLVAGRYEGVDERFVETCIDESLSVGDIVLSGGELPALMLVDAVARLLPGTLG
                     CAESAEQESFAGGLLDWPHYTRPETWQGREVPEVLKGGNHAAIERWRRKQALGRTLER
                     RPDLMRKRPLDDAERKLLDEYRAEQDASRLGQGRRHCEKGET"
     gene            complement(7862..8344)
                     /gene="rimM"
                     /locus_tag="PL152_05510"
     CDS             complement(7862..8344)
                     /gene="rimM"
                     /locus_tag="PL152_05510"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HQR48769.1"
                     /translation="MLGRIGAPFGVQGWVKVTSYTDPAEGIVAYGSWDLVRAGQAERR
                     TVLEWKRAGRAVAVRLEGVGSREAAQALTGCEVQVDRSELRPTAPGEYYWHDLVGLEA
                     LNREGVSLGRVDSVMELPAHPVLVLKGDRERLVPLVPQRLVAVDLDARRVMLDWHPDD
                     "
     gene            complement(8388..8642)
                     /gene="rpsP"
                     /locus_tag="PL152_05515"
     CDS             complement(8388..8642)
                     /gene="rpsP"
                     /locus_tag="PL152_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010484678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HQR48770.1"
                     /translation="MVTIRLMRRGGKKEPFYHVVVTDSRRSGGGTLEQVGFFNPVAKG
                     ASEEVRLDLDRIDHWVSRGAQPSDRVAQLLKGYRKAKAAA"
     gene            complement(8753..10108)
                     /gene="ffh"
                     /locus_tag="PL152_05520"
     CDS             complement(8753..10108)
                     /gene="ffh"
                     /locus_tag="PL152_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HQR48771.1"
                     /translation="MFDKLSSRLAAVVEGLRGRGRLTEENIADTLRQVRMALLEADVA
                     LPVVKAFIDAVKARAVGLEIHKSLTPGQALVRVIHEELVRVMGEGVRPLNLRTQPPAV
                     ILLAGLQGAGKTTTAAKIALWLKDRERKRVLLVSTDVYRPAAMLQLERLGQQLGVDVA
                     HADPSQSPLAIAAGALDEARRGLYDVLLVDTAGRLHVDDAMMQEVRELGASLQPIETL
                     FVVDSMAGQDAVNAARAFGAALDLTGVVLTKADGDARGGAALSVRQVTGRPILFVGVG
                     EKPEALEAFQPERMASRILGMGDVLSLVEEVTRKVDREEAEKLARKVAKGKDFDMNDL
                     LAQLRQLEGMGGISALMEKLPAHLTARAGAAPLGQEREIRRQVAIICSMTPQERRYPK
                     VIDGSRRRRIATGSGTHVADVNRLLKNFLQMQKMMKGMGKGGLGRMMRAMAGRLPPGL
                     G"
BASE COUNT         1513 a         3673 c         3411 g         1665 t
ORIGIN      
        1 agatgtcggg caatccgaag gccttccgcg acctgctgcg caccatgcac cacgggggca
       61 gcgtggcgct gctcggcatc ccgcccgacg acaccgcgat cgactggaac gaggtgatct
      121 tcaagggcct ggtcatcaag ggcgtctacg gacgcgagat gttcgagacc tggtacaaga
      181 tggcggcgat gctgcagagc gggctcgaca tctcgcccat catcacgcac cgctttcccg
      241 tcgccgacta ccgcgagggc ttcgagatca tgcggagcgg ccagtccggc aaggtcatcc
      301 tcgactgggc cgcatgagac gcctcgcgcg gatcgtcgcc tggatcctcg gcggactcgc
      361 ggtcctcatc gcgggctata ccgcggtcac ctgggcgccc gaccgcccgg tcgaggagct
      421 cgtcgcgcgc tgggcgccgc cgccctcgac cttcatcgac ctgcagggca tgaaggtgca
      481 cgtccgcgac gaaggcgcgc gcgacgatcc cgtgccgatc gtgctgctgc acgggacctc
      541 ggactcgctg cacacctggg acggctgggt gcgggcgctc gagggccggc gccgcgtcat
      601 tcgcgccgac atgccgggct ttggcctgac cggcccctcg cccgcgaacg actactcgat
      661 cggcgcctac gtgcgcttcg tcgaggcgct gctcgaccac tacggcatcg agcactgcgt
      721 tctggccggg aattcgctcg gcggctgggt cgcctgggaa acgaccttgg cgcagccgcg
      781 gcgcgtcgac gcgctgatcc tcgtcgacgc cgcgggttac cgctacgaat cgcagtcggt
      841 gccgatcggc ttccggttgg cccggatccc gctcctgaac cggctggtgc tcgtgacgct
      901 gccgcgcagc ctggtcgagt ccagcgtgcg caacgtcttc ggcgacccgg acagggtgac
      961 gagggagatc gtcgaccgtc attacgagct cgcgctgcgc gcgggcaacc gggccgccat
     1021 cgtgcaacgc ttccggcagg ccaaggctgg agtggacgaa gcgaggatcc cggaaatcaa
     1081 ggtcccgacg ttgatcctct ggggaggacg cgacaggctg atcccggccg agtacggcgg
     1141 cctgttcgag cgcgacatcg cgggcagcaa gctcgtggtg ttcgaggacc tgggccacat
     1201 tccgcaggaa gaggacccgg cgcggaccgt cgcggccgta caggacttcc tcgccaccct
     1261 gccacctgcc tccccctgag cggacgcggg cgcccattgt cggcaatcac acgcacaggc
     1321 tgaccgatgg cccgcattcg cctcctccgc tcgctctacc tgcaggtgct ggtcgccgtc
     1381 gtgatcggcg ccgtgatcgg gttcgcgtgg cccgcgacgg gcgaggccct gaaaccgctc
     1441 ggcgacggct tcatcaagct catcaagatg atgatcgggc cgatcatctt ctgcaccgtc
     1501 gtgctcggca tctccggcat ggaggacctg aagaaggtcg gcaagaccgg cgggctggcg
     1561 ctcgtctatt tcgaggtggt gagcacgatc gcgctcgtga tcggcctcgt gatcgtgaac
     1621 gtggtacggc ccggcgcagg cgtgaatgcg gatcccgcaa cgctggatgc gtccgccgtc
     1681 gcgcagtatg cgagccaggc ggagtcccac ggcgtcacgc agtacatcct cgacctggtc
     1741 ccctccacgc tcgtcggcgc cttcgcctcc ggcgaggtgc tgcaggtgct gctggtctcg
     1801 gtgctgttcg gcttcgcgct gcacgcggcc ggcggccggc agctcgcggt gtaccgcctg
     1861 gtcgagcagc tctcggccgt cctgttccgg gtggtcggct tcatcatgcg gctcgcgccg
     1921 ctcggtgcgc tcggcgccat ggccttcacc atcggcaaat acgggctgga cgcgctcgtg
     1981 tcgctcggca aactgatggc gtgcttctac gcgacctgcc tgctcttcat cttcggggtg
     2041 ctcggcggca tcgcccgctg gaatggcttc agcgtctggc gcttcatccg gtacatcaag
     2101 gaagagctct tcatcgtgct cggcacgtct tcgtcggagt ccgtcctgcc gcggatgatg
     2161 acgcgcatgg aggacctcgg cgtcgagcgg acggtggtcg ggctcgtgat cccgaccgga
     2221 tactcgttca acctcgacgg cacggcaatc tacctcacca tggcggccgt gttcatcgcc
     2281 caggccacca acaccccgct cgacctgaca cagcagctca cgctgctcgc cgtcctgctg
     2341 ctcacgtcga agggcgcggc cggtgtcacg ggcagtggct tcatcgtgct ggcggcgaca
     2401 ctgtcggccg tggggcacgt gccggtcgct ggtgtggcgc tgatcctcgg catcgaccgc
     2461 ttcatgtccg aggcgcgctc gctcaccaac ctgatcggca acggcgtggc gaccgtcgtg
     2521 gtcggcaagt ggtgcggtca ggtggacgaa tcgacgctgc gggcgcgact ggcgggcgcc
     2581 gaggaaccct cgtcgagcta gctctggcga atgctgccga ccgcagcggt gcctcgtcca
     2641 ccctggcgcc gcgcgccgtg cagcgactac cggaaccgct atgcccaacc tgttagaacc
     2701 gcttgcattc cagcgcggcc cggccatgag caatcgcctc atgctggcac cgttgacgaa
     2761 tacccagagc cacgacgacg gcacgctgtc ggacgaggag ttccgctggc tcaccatgcg
     2821 ggccgagggc ggcttcggac tcacgatgac ctgcgcttcc cacgtgcagg cgcagggccg
     2881 cgggtttccc gggcaactcg gcatctggtc ggacgcacac atcccggggc tgacgcggct
     2941 cgcggccacg atcaagcggc atggcagcct ggcaatggcg cagttgcacc atgccggaat
     3001 gcgctcaccg gcggagctga tcggccagca gccggtgtgt ccgtcggaca attccgagaa
     3061 aggtgcgcgg gcgctgacgg cggccgaggt cgagcaactg gtcgaggact tcgtactggc
     3121 agcgctgcgg gcggagcgcg ccggattcga cggcgtggaa ctgcacggcg ctcacggcta
     3181 cgtgctggcg caattcctga gcgccgggat caatcatcgg ggcgaccgct acggtggctc
     3241 gctcgagaat cgcatgcgaa tcctgttcga catcctgcac gggatacgca cacgctgccg
     3301 gccggatttc acggtcgggg tgcgattgtc gcccgagcgc ttcgacctga ggctcgcgga
     3361 gatggcggtg gtcgcacggg aactgatggc gagcgggctg atcgacttcc tcgacctgtc
     3421 gctctgggac gtgttcaagg agccggagga gcccgagttc aagggccgca gcctgatgtc
     3481 gtacttcacc gggctcgagc gcggcaaggt gcgggtaggc gtcgccggca ggatctcgag
     3541 cggtccggat gcccgacact gcctcgatgc gggcgccgac ttcgcgatca tcggccgcgc
     3601 ggcgatcctg caccatgact tcccgcagag agtgcggacg gaccctggat tcaaggcggt
     3661 ggggctgccg gtcacggccg actacctcgc gtccgagggt ctctcgccgc cgttcatccg
     3721 ctacatgggg aactggaagg ggttcgtggc ggccgcctga caccgcaggt ccgggaggat
     3781 tgtctctccg cttaccacgc accaggccga gcggcgctgc cgcgcggaca actgtgtcat
     3841 gattgctgcg gctcgatccg gggagagccg tgcggacctc acgtcgcggg ggaaccatga
     3901 gcggcagcaa cgagcgagaa ggcggacgcg cgtccggcga cctgcccgcc ggcggcaacg
     3961 ccgtcgtcat cgaagcctgg aacaccgtcc tctacgagaa gttctgccgc ttcaagcacc
     4021 tgctcgtcga gggcctgtcg cagcacagcg acgcggcgct cgagcgctgc cggtaccagc
     4081 ccggcgcacg cgtcctcgac gtcggctgcg gtttcggcga cagcacgctg cgcatcgccg
     4141 acaaggtggc tcccggtggc gaagccgtcg gcgtggactg cgccgagaac ttcgtccgcg
     4201 gtgccgcgga cgaggcgcgg gaagccggca tccagaacgc gcgatttttc gctgccgacg
     4261 tgcagtccga cgacctgcag ggcccctacg accacgcatt cgcgcgattc ggcacaatgt
     4321 tcttcgacat gcccggagct gccatgcgca acatccgcaa ggcattgaag cctggcggca
     4381 cgttcatgca gatcgtctgg cgccgccgcg aggacaaccc ctggctgtac gaggccgagt
     4441 tgcgcgtgaa ggcgatcgtg cccgccgtct cgcacgagga gaccaaccag gtgcattgcg
     4501 ggccgggacc gttctccatg tccgggcccg acatggtcag ctcgatgttg accgccgcgg
     4561 gattcgagcg catcgccttc gagcgctacg actgcgacat ctgcatcggc cgcgacctga
     4621 acgacgccgt ggagttcgcg atggcgctgg gcccggccgg cgaggccatg cgcctggcgg
     4681 gtgaggccgc cgaacggctg aagcccgagg tcatcgcggc gctggaggca gcgctcgcgc
     4741 cgtacgtgcg gccggacggc gtgtgggctc cgtcgagcac gtggttcatc agcgcgacga
     4801 gtcccggctg accccggcgc gaatcagtcg atcgcgcagc ccaggcaata ggcgtaggta
     4861 ttccccgggt cccggagccg ctcgagacgc tgcacttcct tcagcagcgg agccagggcc
     4921 ttcgggacca cgggcagcgc ggacttcggc agcagcggcg cgaccacccg ggcgactgcg
     4981 atgcgtaccc tgagcccgag cgcctcgccg atgctcacgt cgtcgtcgta gtactcgacg
     5041 tcgtagtcat cgccgagctt ggcgagcccc gcggcgacgt cgatcgcgtc ctcgtagccc
     5101 ccgagctggt ccacgagtcc gactttcttc gcatcggatc ccgcccacac gcgtccctgg
     5161 gcgaccttgt ccacggcttc gtaggacatc gtgcgtgcct tcgccacgtc accgacgaag
     5221 tccctgtagc cgcgctcgat cgtctgctgg atcaggtcct tcgagaggtc gctcatgggc
     5281 cggtcgagcc ggaactcacc ggcgagcgcc gtcgtgccga cgccatccgt gtgcaccccg
     5341 agccgctcga gcgagcgctg gaacgtgggc atcatgaaga agatcccgat cgagccggtc
     5401 agcgtggccg ggctggccac gatcttgtcc gcggccatcg cgatgtaata gccgcccgag
     5461 gcggcgaggc tgctcatcga ggcgaccacg ggcttgccgc tcttgcgcac cgcctcgacc
     5521 tcgcgacgga tcacgtcgct cgcgaacacg ctgcccccgg ggctgtcgat gcgcagcacg
     5581 accgctttca cttcctcgtc gttggcggca tcacgcagct cgccggcgag cgtgtcggcg
     5641 ccgatcatgc ccggcaactc ctcgcccggc acgatgtcgc cggacgccac gatcacggcg
     5701 accttctgct tcgatttcga cgtgagcgcc tgctcggagt ccacgttcgc gaggtaggag
     5761 tcgaaatcca cgccgatgta ggaatgcgtg tcctcgtcct cgccggtgac ctgcgcgagg
     5821 cggtcctcga cgtcgagccg gcccttgagc gaggtgacga gccccgcatc cagcgccatc
     5881 ttcgccaggt cgccgtccgc cttgcgcagc agcgcggccg actcgtccgc gtacgactgc
     5941 aggcggtccg gctcgaattc gcgcgccgcc gcgacgtccg acttgtaggt gttccacgcc
     6001 gcgcccagcc acccgacgct ttcctcgcgc gaggccggcg acatgtcgtt gcggctgaag
     6061 atctcgacgg ccgacttgta ctcgccgacg cggaatatgt tgacgtcgac cgacagcttg
     6121 tcgagcgcgt ccttcacgaa caggtcgtag ttgtcgtagc cgtcgacgta ggccgcgccc
     6181 atcgggtcga ggtagatctc gtcggcgtgc gccgcgaggt agtactggtt ctgctcgtag
     6241 tagtcgccgt aggcgatgac cggcttgccg gacgcgcgga agtcgtcgat cgcggcggcg
     6301 acttcctgca gcttcgcgag gccgccgccc tcgaggccgc cgagatcgag atacagcgaa
     6361 ctgatgcggt cgtcgtccgc ggccgactcg atggcgtcca cgacgtcgcg cagcagggtc
     6421 tccttcggct cggtgcgcag cgccttcgac agcgcccgct cgaccgggtc cccggtgagc
     6481 tgctcgacca gggggccgtg cgggtcgatg accagcgcgg acttgtcgcg gacgagcggg
     6541 atgggtgcgg agaacagccc gacgacgagg ctgaacagca ccagcagcaa tacgaggtgc
     6601 aggaccttgc gcagcccgtc gaggccgctc cagatccagc gcaggacttt gccgatcgcg
     6661 cggaacattc gctacccggt gcgcgggtgc ggcgagcgca cccgtgcgtc cggacggagc
     6721 gtcagacctt ctcttcgatg cgcgcagcct tgcccgtgcg atcgcgcagg aagtacagct
     6781 tcgcgcggcg cacgtgcccg cggcgcttga ccgtgatcgc gccgagcgac gggctgtagg
     6841 tctggaacac gcgctccacg ccctcgccgt gcgagatctt gcgcacggtg aacgacgagt
     6901 tgaggccgcg gttgctcttc gcgatgacca cgccttcgta ggcctggacg cgctcgcggt
     6961 cgccttcctt gaccttcacc tcgacgacga cggtgtcgcc cgggccgaag tccggaacct
     7021 cgcgctgcat gcgctccttc tcgagcgtgg caatgatgtt gctcatgtct cacctttctc
     7081 gcagtgtctg cgtccctgcc ccaaccgcga agcatcctgc tccgctcggt attcgtcgag
     7141 gagcttgcgc tccgcgtcat ccaacggccg cttccgcatc aggtccgggc gccgttccag
     7201 cgtccgcccg agcgcctgct tgcggcgcca gcgctcgatc gcggcatggt tgccgccctt
     7261 gagcacttcc ggcacctcgc gcccctgcca cgtctccggc cgcgtgtagt gcggccagtc
     7321 gagcagcccg ccggcgaacg attcctgttc ggccgactcg gcgcagccga gcgttcccgg
     7381 cagcagcctc gccaccgcgt cgaccagcat cagcgccggc agctccccgc cggacagcac
     7441 gatgtcgccg accgacagcg actcgtcgat gcaggtctcg acgaaccgct cgtcgacgcc
     7501 ctcgtaccgg cccgcgacca gcaccagctc accgagggcc gccagttcgg cgaccttccg
     7561 ctgcgtcacc ggtgtgccgt cggcggcaag gtacacccgc cgcgcctccg gcacgagccg
     7621 ctccgcctcg accatggcga gcctcaacgg ctccgccttc aacaccatcc cggggccgcc
     7681 gccgtagggc cggtcgtcca ccgtcttgtg cacgtccgtg gcgaactgcc tggggttcac
     7741 cgtccggacc tcgagcacgc cgcgctccag tgcccggccg agcacgccga agcgcacggc
     7801 ctgctcgacg aactcgggaa acaacgtgac gaccacgatc ttcacgcacg ctcccgcccc
     7861 gtcaatcgtc cgggtgccag tccagcatca cccgtctcgc atcgaggtcc acggcgacca
     7921 gcctctgcgg gaccaggggc accagtcgtt cccggtctcc cttcagcacg agcaccgggt
     7981 gggccggcag ttccatcacg ctgtcgactc gcccgaggga aacgccctcg cggttcagcg
     8041 cctcgagacc caccaggtcg tgccagtagt actcgcccgg cgcggtcggc cgcagctctg
     8101 agcggtccac ctggacctcg cagcccgtca acgcctgggc cgcctcgcgc gaccccacac
     8161 cctccaggcg gacggccacg gcacgccctg cccgcttcca ctccaggacg gtccgccgct
     8221 cggcttgtcc cgcccggacc aggtcccatg atccgtacgc gacgataccc tccgctggat
     8281 cggtgtagga cgtgaccttc acccaaccct gcaccccgaa cggggcgccg atgcggccga
     8341 gcaccactgc gcgccggtca ctgccggggc gcgtaccgcc catccggtca ggccgcggcc
     8401 ttggccttgc gatagccctt gaggagctgc gcgacccgat ccgaaggctg ggcgccacgg
     8461 ctcacccagt ggtcgatgcg gtcgaggtcg agtcgcacct cttcgctcgc gcccttggcg
     8521 accgggttga agaacccgac ctgctccagg gtgccaccgc cgctgcggcg gctgtcggtc
     8581 acgaccacgt ggtagaaggg ctccttcttg ccaccgcggc gcatcaagcg gatcgtcacc
     8641 atcgtcagac tatgcctcgt tcagggcggc ccggtgcctg cacggcgccc ggccaaaaaa
     8701 gagccgcgaa ctataccgaa aatccctgcc gaaagaaatg cttgtggcgg cctcagccga
     8761 ggccgggcgg caggcggccg gccatggccc gcatcatgcg gccaaggccc cccttgccca
     8821 tgcccttcat catcttctgc atctgcagga agttcttgag cagtcggttc acgtcggcca
     8881 cgtgggtgcc cgaccctgtc gcgatccggc ggcggcgcga gccgtcgatg accttcgggt
     8941 agcggcgctc ctgcggggtc atggagcaga tgatggcgac ctggcgccgg atttcgcgct
     9001 cctggccgag cggcgccgct ccggcgcgcg cggtcaggtg cgccgggagc ttctccatca
     9061 gggccgaaat gccgcccatg ccctcgagct gccgcagctg ggccagcagg tcgttcatgt
     9121 cgaagtcctt gcccttcgcg accttgcggg cgagcttctc ggcctcctcg cgatcgacct
     9181 tgcgggtgac ctcctcgacg aggctcagca cgtcgcccat ccccaggatg cgggacgcca
     9241 tgcgctccgg ctggaaggcc tcgagggcct cgggcttctc gccgacgccg acgaacagga
     9301 tcggccgccc cgtgacctgc cgcaccgaca acgcggcgcc cccccgcgcg tcgccatcgg
     9361 ctttggtgag caccacgccc gtgaggtcga gcgccgcccc gaacgcccgg gccgcattca
     9421 ccgcgtcctg gcccgccatg ctgtcgacga cgaagagcgt ctcgatgggc tggagcgacg
     9481 cgccgagttc gcgcacctcc tgcatcatcg cgtcgtcgac gtgcaatcgc cccgccgtgt
     9541 cgacgagcag cacgtcgtag agtccgcgac gcgcctcgtc cagggcaccc gcggcgatcg
     9601 ccagcggcga ctgcgacgga tcggcgtggg ccacgtccac gccgagctgc tgcccgagcc
     9661 gctcaagctg cagcatggcg gccggccggt acacgtcggt cgagacgagc agcacccgct
     9721 tgcgctcccg atccttgagc cagagcgcga tcttcgcggc cgtggtcgtc ttgcccgcgc
     9781 cctgcagccc cgcgagcagg atcacggccg gcggctgcgt gcgcaggttc aacggccgca
     9841 cgccctcgcc catgacccgc acgagttcct cgtggatcac gcgcaccagt gcctgtcccg
     9901 gcgtcaggct cttgtggatc tcgaggccga ccgcgcgcgc cttgaccgca tcgatgaacg
     9961 ccttgaccac cggcagcgcc acgtccgcct cgagcagcgc catccgcact tgccgcagcg
    10021 tgtcggcgat gttctcctcg gtgaggcgcc cgcggccgcg cagtccctcg acgacggcgg
    10081 cgaggcggga ggaaagcttg tcgaacacgg tggtccccgg atccggtaat gctgcgcgtg
    10141 cggcggagcg cggattatag aggccgcgcc tcctcacgcc agcgccgttg tgccatcatt
    10201 tcacggtcgc cgctgcaccg gacccgcgct tgctcctgcc cctcatcgcc gtcgcctgct
    10261 ac
//
LOCUS       DAOMFD010000065        10166 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1870_length_10166_cov_33.141727, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000065 DAOMFD010000000
VERSION     DAOMFD010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10166)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10166
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1870_length_10166_cov_33.141727"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            339..1661
                     /locus_tag="PL152_05525"
     CDS             339..1661
                     /locus_tag="PL152_05525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48772.1"
                     /translation="MAHRRFASIVSLAAWSVAAAGPAVAQSQPKQVVKPPISQAWIDV
                     ATFSGFGMGGGMGPGSGSGQGMMGMTLGAMMGGHAAQAEFGNTQTGTTGRWMDVTLYT
                     SRNPNLQQATQSVPTVTGLAPTLKLMTPERPKPAPKDDDTPTDFNYEPPKGKFVLYWG
                     CSETVRPGQPKVVDLQTSTMAEFAKFFESRRSTTRGAHATVGRPIWPSKDDKRVFPAG
                     ASIVGQHAYQGDGVPESFKFAIPAAQDIMPAVQLKQSDQGKFIKLDWNAIPAARAYFL
                     GSMGAREGEQNTIVIWTSSELPDSGFGLFDYQTNAAVDKWLKDKVLLPPTTTTCAIPK
                     EAAGQGMLRAIAYGSELNMAYPPRPTDPNVPWEPDWNVKIRVKSMTTTMVGMPAGSMP
                     GAEGADTEGMTDAPPPGEGQPAQPAEPPKKKKSWNPLEVLKDVVEGNH"
     gene            complement(1745..3973)
                     /locus_tag="PL152_05530"
     CDS             complement(1745..3973)
                     /locus_tag="PL152_05530"
                     /inference="COORDINATES: protein motif:HMM:NF012602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HQR48773.1"
                     /translation="MAEHQLHHRTCPICEAMCGIVVEHDGGKVISIKADRDDVLSRGH
                     ICPKAVALKDIHEDPDRLRRPVRRTDGGWQEISWPDAFDEVERRIGDVRARFGKDAVA
                     IYAGNPIVHNLGAMLGIGDFIRAMRTRNLYSATSVDQLPHMMASQMMFGHQFLVPVPD
                     VDRTQLFVCIGGNPVASGGSIMSAPGFEKRIEALRARGGRFIVIDPRRTESAQIADEY
                     LPIRPGTDVYLLLALLREIFDGGFASPGHLQAQLDGVETLHRAVMQFDPPRLAERTGI
                     PLARIKKLAREIAAEPRALVYGRVGACTQEFGGLTLWLIYCLNLLTRHMDAVGGMMFA
                     EPAVDLTRAYGSPGHRDRFRSRVRGLPEFGNELPVATLADEILTEGKGQVRALVTVAG
                     NPVLSTPNGRQLDRALAGLDFMVAVDPYINETTRHAHVILPPTSPLEHGHYDVALSGF
                     AVRNVVKYAPALFEPPPGALHDDEILGELTLRLRTQPGLERRLASARGLVARRLGPDG
                     LLDWMLRTGRYGQENRGPLAMLARLPGFGAMRRELEAPDRRPQGLSLARLRASPHGVD
                     LGPLEPCLQRRLATADRRIRLAPDAYVADLARAARALDAPPADLVLIGRRHVRSNNSW
                     LHNSHRLVKGKPRCTLMIHPDDAMRRGIADGGIVRLKSRVGAVEVAAEITTDVMPGVV
                     SLPHGWGHDRDGARLSVAARTPGVSINDIIDDRRIDELSGTAVLNGTPVHVEPAAPAA
                     GT"
     gene            complement(4007..5440)
                     /locus_tag="PL152_05535"
     CDS             complement(4007..5440)
                     /locus_tag="PL152_05535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48774.1"
                     /translation="MTMMSLRRVLPIRPDRLARVPSAAALLMITSAAFGQTAPAVSTI
                     VAFNLSVPSGGVVMGTTDGALYGTGTSRTIVSGGVVYRATRDGLSVKTLHQFQGSEGS
                     TPAAGVIEGSDGLLYGSTSYGAADQAYSTGTIYRLKKDGTDFTVLYRFASYTTVNVVN
                     MPVNTDGARPESQLLEGSDGNLYGVTAAGGANGTGVVFRMTMDGTAFQLLHEFGAVTS
                     SATDSVIKNDDGVTPVGRLLQAPDGYLYGTTSNGGVDGRGVIFRVHMDGSGFEVVHQF
                     PALSSGSPAINVDGARPVGGLTDGQNGLLYGIANLGGANGVGTLYSLDPNSLLLTVLH
                     DFDAPEGAFPAGGLTLALDGRLYGTTQGGGTNSSGAETTAGTIFSVALDGTGFTKHYS
                     FDGSQGASPNTRLLQLDDTTFAGVTPSGGACSQGTLYSFSLTGATVVGNTTCGQKKKN
                     KSGGGATDPLVVLIGAALGLVSRRRRG"
     gene            5587..7494
                     /locus_tag="PL152_05540"
     CDS             5587..7494
                     /locus_tag="PL152_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR48775.1"
                     /translation="MILLRHTTLARGARPLIEDASLQIHPGWRVGLVGANGSGKSSLF
                     ALLRGELHAESGDCEVPSAWHIASVSQETPPLDQPAIEFVLDGDVELRNVERALAEAE
                     RDHDGHAVAECHARLDAIDGYSARARVAAMLAGLGFGTADLERPVADFSGGWRMRLNL
                     GRALASRADLLLLDEPTNHLDLDAVVWLERWLANWRGTLLVVSHDRDFLDGCVSHVAQ
                     IESRRLTLYAGNYSAFEQQRAARLAVQQALYEKQQREIAHMTDFVRRFRAKASKARQA
                     QSRLKALDRLERIAPAHVDAPFDFEIPAPERSPDPLLALDDANLGYAGRPVLEDVSLS
                     LRPGARLGLLGPNGAGKSTLVKSIAGEIPVLAGQRTEGHGLRIGYFAQHQLEQLRPDE
                     SPLQHLARIDGQARELDLRTYLGSFDFRGSMADAPVGAFSGGEKSRLALALIIRQRPN
                     LLLLDEPTNHLDLEMRYALTRALAGYEGSIVLVSHDRSLLRTVCDSFLLVADGRAEEF
                     SGDLDDYLAWLSERRARSGARLAPEAATGREIRRAQRETSHAERQARLARRRPLAREA
                     GALEREVTALEAEKRELEARLSDAAFYAGGNGAEVQAATRRCAEIARRLEQAEARWLE
                     LQGELEAIGDA"
     gene            complement(7520..8494)
                     /locus_tag="PL152_05545"
     CDS             complement(7520..8494)
                     /locus_tag="PL152_05545"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HQR48776.1"
                     /translation="MRAVVCRELTGIEGLRLEADWPEPVAAPGEVVVDVKAAALNFPD
                     LLTIRGLYQERPPLPFVVGSEMSGVVSSVGEGVKHLRVGDRVVAACGRTLAERVAARA
                     SLVVPAPPGLSFEAAAGVCITYFTTLHALKQRASLAPGETLLVLGAAGGVGTTAVELG
                     REMGATVIAAASTAEKLELARSLGAAHLVNYSTEDLRQRLKEITGGKGVDVVYDPVGG
                     DLAEPALRSMAWGGRYLVIGFAAGQIPSIPLNLPLLKGCSIVGVFWGSFVQREPEVQK
                     RNVAELWEMFQSGRLKPVVGETHDLADYAAAFESLQARRAIGKVVVTP"
     gene            complement(8612..9781)
                     /locus_tag="PL152_05550"
     CDS             complement(8612..9781)
                     /locus_tag="PL152_05550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HQR48777.1"
                     /translation="MKRQHYAGRDFRRAITIEDLRRVARRRLPNFSYEYVEGGAEDEV
                     TLRRNRDVFEKITWQPRALVGAGPPDMGSEIFGKPVNLPIVIAPTGFNGLLWKQGDIA
                     LASAAREAGVPFTLSTASNCSVARLAAAVQGNHWYQLYAYKDQNVVDRLTDHVREAGC
                     EVLVITADAPVLGAREWDQRNYSAPMQLSLASKLDVLSHPGWLARVMFPSGAPTFENL
                     TEFLPPGKYSALIGAKYSFSQINSRLSWRDVERIRARWPGKLVVKGVLCIEDARRAAE
                     AGCDGVVLSNHGGRQLDTCVSGVELLPAVASELRDRLTVLVDGGFRRGSDVLKAIALG
                     AHGVMIGRAALYGLAAGGEAGVRHAIGILRTEMERTMLLLGCGSIPELGRHLIRT"
     gene            complement(9778..>10166)
                     /locus_tag="PL152_05555"
     CDS             complement(9778..>10166)
                     /locus_tag="PL152_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48778.1"
                     /translation="FSGELDIANARIRYANGCVANTTASRISMKVERKLRLFQDDAYI
                     SIDLQQKILTIVRKPPEGARAAPGQVSIEERGYEQGDALRLEIEAFLRSVRDGTPPVV
                     SGEDGLHALETALRITELVHDRAGRA"
BASE COUNT         1606 a         3611 c         3441 g         1508 t
ORIGIN      
        1 ggttgctgca ttcggcgagg ctgctcatgt cgagcacggt cctgccgtcc tcgagatgca
       61 ggcgcgcgcc ggcaccgccg acgaccgtcg gggcatccca atgcgactgg acgcaccagc
      121 tgtgcaggac ggtccgccgt tcgatgctca actgtccccc agggggcgga cggtggcgag
      181 gtaccgcgcc cgtcctgatt acgtattggc ccgcaattct acaacgaggc cgtggggcac
      241 cggcatcggc ggcgggtccg taccccacga agacttcgcc ttgtggcggc gcccgacctg
      301 cgcgaccatg tcgacgctag gctcaacggg ggacgatcat ggcacaccgt cgttttgcgt
      361 cgatcgtgtc gctggccgca tggagcgtcg ccgccgccgg cccggcagtc gcgcagtccc
      421 aaccgaagca ggtcgtcaag ccaccgattt cccaggcctg gatcgatgtg gcgacgttct
      481 ccgggttcgg catgggcggg ggcatgggac cagggtctgg ctctggccag ggcatgatgg
      541 gcatgaccct gggggccatg atgggcggcc atgccgcgca agccgagttc ggcaacacgc
      601 agaccggcac caccgggcgc tggatggacg tgaccctcta cacgagccgc aatccgaacc
      661 tgcagcaggc gacgcagtcg gtccccacgg tgaccggact cgcgccgacg ctcaagctga
      721 tgacgccgga gcggccgaag ccggctccca aggacgatga cacccccacc gatttcaact
      781 acgaaccgcc caagggcaag ttcgttctgt actggggttg ctcggaaacc gtgcgcccgg
      841 gacagccgaa ggtcgtggac ctccagacgt ccaccatggc ggaattcgcc aagttcttcg
      901 agtcacggcg ctccaccact cgcggggcgc atgcgaccgt cggccgcccg atctggccga
      961 gcaaggacga caagcgtgta ttcccagccg gcgcctcgat cgtcggccag cacgcttacc
     1021 agggcgacgg cgtgccggag agcttcaagt tcgcgattcc cgccgcgcag gacatcatgc
     1081 cagcggtgca actcaagcaa tccgatcagg gcaagttcat caagctcgac tggaatgcga
     1141 tcccggccgc gcgcgcgtac ttcctcggca gcatgggcgc gagggaaggc gaacagaaca
     1201 ccatcgtgat ctggacctcg agcgaactgc cggacagcgg cttcggcctg ttcgactacc
     1261 agaccaatgc ggccgtcgac aagtggctca aggacaaggt cctgctgccg ccgaccacga
     1321 cgacctgcgc gatcccgaag gaggcggccg gccagggcat gctccgcgcg atcgcgtatg
     1381 gcagtgagct caacatggcg tatccgccgc gccccaccga tccgaacgtc ccgtgggagc
     1441 cggactggaa cgtgaagatc cgcgtgaagt cgatgaccac gaccatggtc ggcatgcccg
     1501 caggatccat gcccggcgcc gaaggcgccg acaccgaggg catgaccgac gcaccgccgc
     1561 ctggggaggg ccagccggcg caacccgccg agccgccgaa gaagaagaag agctggaacc
     1621 cgctcgaggt gctgaaggac gtcgtggagg gcaaccacta ggccaggggc ggcgaccttg
     1681 ccgtgctgat caggaagcgg cggtagccac cggcgcacgg ctgagccgaa ccgcacggcg
     1741 acgttcaggt accggcggct ggtgcggccg gctcgacgtg gaccggcgtg ccattcaaaa
     1801 cggccgtgcc cgacagttcg tcgatacgcc gatcgtcgat gatgtcgttg atgctgacgc
     1861 ccggcgttcg cgcggcgacc gacagacgcg ctccgtcccg atcgtgcccc cacccgtgcg
     1921 gcaggctcac cacacccggc atgacgtcgg tcgtgatctc cgccgcgacc tcgacggcgc
     1981 cgaccctgga cttgaggcgc actatgccgc catcggcgat cccgcgccgc atcgcatcgt
     2041 cggggtggat catcagcgtg cagcgcggct tgcccttcac cagccggtga ctgttgtgca
     2101 gccacgagtt gttgctgcgc acgtgccggc gtccgatgag cacgagatcg gctgggggcg
     2161 cgtcgagtgc gcgcgccgcg cgcgcaaggt cggcgacgta cgcatcgggc gccagtcgga
     2221 tgcgccgatc cgcggtcgcg agcctgcgct gcaggcaggg ttccagcggt cccaggtcga
     2281 cgccgtgcgg cgaggcgcgt agccgcgcca gcgacagccc ctgcggtctc cggtcgggcg
     2341 cctcgagttc gcgacgcatc gcgccgaagc ccggcagtcg cgccagcatt gccagcggac
     2401 cgcggttctc ctggccgtag cgcccggtgc gcagcatcca gtcgagcagc ccgtccggcc
     2461 ccaggcggcg cgcgacgagg ccgcgggcac tggcgagtcg gcgctcgaga cccggctgcg
     2521 tgcgcagccg caacgtcagt tctcccagga tctcgtcgtc gtgcagcgcg ccgggcggcg
     2581 gctcgaacaa cgcgggcgcg tacttgacga cgttgcggac cgcgaacccc gagagcgcga
     2641 cgtcgtagtg gccgtgctcg agcgggctcg tcggcggcag gatcacgtgc gcgtggcgcg
     2701 tcgtctcgtt gatgtacggg tccacggcga ccatgaaatc caggccggcc agcgcgcggt
     2761 cgagctggcg cccgttgggc gtggacagga ccggattgcc ggcgaccgtg acgagcgcgc
     2821 ggacctgccc cttgccctcg gtgagtatct cgtcggcgag ggtcgcgacc ggcagctcgt
     2881 tgccgaactc gggcaggccg cgcacccgcg accggaaccg gtcgcggtgt cccggcgagc
     2941 cgtaggcgcg ggtcaagtcg accgctggtt ccgcgaacat catgccgccc acggcgtcca
     3001 tgtgccgcgt cagcaggttc aggcagtaga tgagccagag cgtcaaaccg ccgaactcct
     3061 gcgtgcacgc gccgacgcgt ccgtagacga gcgcgcgggg ctccgcggca atctcgcgtg
     3121 cgagcttctt gatgcgcgcg agcggaatcc cggtccgctc ggcaagccgt ggcggatcga
     3181 actgcatcac ggcccggtgc agcgtctcca cgccgtcgag ctgcgcctgc aggtgaccag
     3241 gactcgcgaa gccaccatcg aagatttcgc gcagcagggc cagcagcagg tacacgtccg
     3301 tgccggggcg gatgggcagg tactcgtcgg caatctgcgc cgactcggtc cgccgcgggt
     3361 cgatgacgat gaatcggccg ccgcgcgccc ggagcgcctc gatgcgcttc tcgaacccgg
     3421 gtgcgctcat gatcgagccg ccggacgcga cgggatttcc gccgatgcac acgaacaact
     3481 gcgtccggtc gacgtcgggc accggcacca ggaactggtg accgaacatc atctgcgacg
     3541 ccatcatgtg cggcagctgg tcgacgctcg tggcggaata caggttgcgc gtgcgcatcg
     3601 cgcggatgaa gtcgccgatg cccagcatcg ccccgaggtt gtgcacgatc gggttgccgg
     3661 cgtagatggc cacggcgtcc ttgccgaacc tcgcgcgcac gtcgccgatg cgccgctcga
     3721 cctcgtcgaa cgcatcgggc catgaaatct cctgccaccc gccgtcggtg cgacgcaccg
     3781 gccggcgcag acgatcggga tcctcgtgga tgtccttgag cgccacggct ttcgggcaga
     3841 tgtggccgcg actcagcacg tcgtcgcgat cagccttgat cgagatgacc ttgccgccgt
     3901 catgctccac gacgatgccg cacatcgcct cgcagatcgg gcaggtccgg tgatgcagct
     3961 ggtgctccgc catctcgctg ccctcgcctt ctagatcaac cgtcgatcag cccctgcggc
     4021 gacgactcac gaggccgagg gccgcgccga tcagcaccac caacggatcg gtggcaccgc
     4081 cgcccgactt gttcttcttc ttctgtccgc acgtcgtgtt cccgacgacc gtcgcgccgg
     4141 tgaggctgaa gctgtacaag gtgccctggc tgcaagcgcc gccggacggc gtgactcccg
     4201 cgaaggtggt gtcgtccagc tggagcaggc gcgtgttggg gctcgcgccc tggctcccgt
     4261 cgaaactgta gtgcttcgtg aagccggtcc cgtccagcgc cacggaaaag atcgtcccgg
     4321 cggtcgtctc cgccccgctc gaattcgtgc cgcccccctg ggtcgtgccg tagagtcggc
     4381 cgtcgagcgc caacgtcagt ccaccggcgg gaaatgcgcc ctcgggtgcg tcgaagtcgt
     4441 ggagcacggt cagcagcaac gagttcggat cgagtgagta caaggtgccc acgccgttgg
     4501 cgccacccag gttcgcgatg ccgtagagca atccgttctg cccgtcggtc aggccgccga
     4561 ccgggcgcgc gccatccacg ttgatcgccg gactgccgct ggagagagcc gggaactggt
     4621 gcacgacttc gaatccactg ccgtccatgt gcacccggaa gatgacgccg cgtccgtcga
     4681 cgccaccatt gctcgtcgtg ccgtacaggt aaccgtccgg ggcctgcagc aggcggccga
     4741 ccggcgtgac gccatcgtcg ttcttgatca cggagtccgt cgcactcgag gtgaccgcgc
     4801 cgaactcgtg cagcaactgg aatgccgtcc cgtccatggt catgcggaac acgaccccgg
     4861 taccgttggc accaccggcc gccgtcacgc catacaggtt cccgtcgctg ccctcgagca
     4921 attgcgactc gggacgcgcg ccgtccgtat tcaccggcat gttcaccacg ttgaccgtgg
     4981 tgtagctggc aaagcggtac aggacggtga aatccgtgcc gtccttcttg aggcgataga
     5041 tcgtcccggt ggagtacgcc tggtccgccg cgccgtagct cgtagagccg tacagcaggc
     5101 cgtcgctgcc ttcgatcacg ccggccgcgg gcgtggaacc ttccgatccc tggaactggt
     5161 gcagggtctt caccgacagt ccgtcgcgcg tggcgcgata gacgaccccg cccgacacga
     5221 tggtccggga ggtgccggta ccgtacagcg ctccatcggt ggtgcccatc accacgccgc
     5281 cactcggcac cgacaggttg aaggcaacga tcgtggacac ggcgggcgcc gtctgcccga
     5341 aggcagctga agtgatcatc aggagcgcgg ccgcgctcgg gactcgggcc aggcgatcgg
     5401 gcctgatcgg cagcacgcga cgcaacgaca tcatcgtcat tcggtctcca gggtcccggg
     5461 cagggctgcc cgacggggaa ggttgcggat tgtgccggat tccggcgaga agcgcatgac
     5521 gcgggcctga ccgcggtcca gtcgctagaa tgcgcgcccc gattcccgcc gcggagcgcg
     5581 ccggccttga tcctgctacg gcacacgacg ctggcacgcg gcgccaggcc actgatcgag
     5641 gacgccagcc tgcagatcca cccgggctgg cgcgtcgggc tggtcggcgc gaacggcagc
     5701 ggcaagtcga gcctgttcgc gttgctgcgc ggcgagttgc acgccgagtc cggggactgc
     5761 gaagttccct ccgcgtggca cattgccagc gtcagccagg agaccccgcc cctcgaccag
     5821 ccagccatcg agttcgtgct cgacggcgac gtcgagttgc ggaacgtcga gcgggccctt
     5881 gccgaggccg agcgcgatca cgatggccac gccgtggccg agtgccatgc aaggctggac
     5941 gcgatcgacg ggtactcggc gcgcgcacgc gtggccgcca tgctcgccgg gctcggattc
     6001 gggaccgccg atctcgagcg tccggtcgcg gacttctccg gcggatggcg catgcgcctc
     6061 aacctcggac gcgcgctcgc gagccgcgcc gacctcctgc tgctggacga gcccaccaac
     6121 cacctcgacc tagacgccgt ggtctggctc gagcgctggc tcgccaactg gcgcggcacg
     6181 ctgctggtgg tgtcccacga ccgggacttc ctcgacggct gcgtgtcgca cgtcgcgcag
     6241 atcgagtcgc ggcgcctgac cctctatgcg ggcaactact ccgcgttcga gcagcagcgt
     6301 gccgcgcgcc tcgccgtgca gcaggccctg tacgagaagc agcagcgcga aatcgcgcac
     6361 atgacggatt tcgtgcgccg attccgtgcc aaggcgagca aggcgcggca ggcccagagc
     6421 cggctcaagg cgctcgaccg gctggaacgc atcgcgcccg cgcacgtcga cgctccgttc
     6481 gacttcgaga ttccggcgcc cgagcgctcg ccggacccgc tgctcgcgct cgacgacgcg
     6541 aacctgggct acgccgggcg gcccgtgctc gaggacgtga gcctgtcgct gcgtccgggg
     6601 gcgcggctcg ggctgctggg accgaacggc gcagggaaat ccacgctggt gaaatccatt
     6661 gccggcgaga ttccggtgct cgcggggcag cggaccgagg gccacggcct gaggatcgga
     6721 tactttgcgc agcaccagct cgagcaattg cggcctgacg agtcgccgct gcagcacctc
     6781 gcccgcatcg acgggcaggc gcgtgagctc gatctccgga cctacctcgg cagcttcgat
     6841 ttccgcggca gcatggccga tgcacccgtc ggcgccttct ccggcggcga gaagtcacgc
     6901 ctggccctgg cgctcatcat caggcagcgg cccaacctgc tgctgctcga cgagcccacc
     6961 aaccacctcg acctcgagat gcgctacgca ctgacccgcg cgctcgccgg ctacgagggc
     7021 agcatcgtcc tcgtgtccca cgaccgctcg ctgctgcgca ccgtgtgcga cagtttcctg
     7081 ctggtggccg acggccgcgc cgaggagttc tccggcgacc tggacgacta cctcgcctgg
     7141 ctctcggagc gacgcgcgcg cagcggcgcc agactggcgc ccgaagccgc gacgggccgg
     7201 gagatccgac gcgcgcaacg ggagacctcg catgcggaac gccaggcacg cctggcgcgc
     7261 cggcgaccgc tggcccggga agccggcgcc ctggaacgcg aggtcaccgc cctcgaggcg
     7321 gagaagcggg aactcgaggc gcgcctgtcc gacgctgcgt tctacgcggg cggcaatggc
     7381 gcggaagtgc aagcggcaac ccggcgctgc gccgaaatcg cccgcaggct cgagcaggcc
     7441 gaagcgcgct ggctggaact gcagggcgag ctcgaggcga tcggcgacgc ctgatgccag
     7501 ccacgcgggt cgcagcgcct cagggcgtca ccaccacctt gccgatggca cgccgtgcct
     7561 ggagcgactc gaacgctgcg gcgtagtccg cgaggtcatg cgtctccccg acgaccggct
     7621 tcagccgacc cgactggaac atctcccaga gttcggccac gttgcgcttc tgcacctcgg
     7681 gctcccgctg gacgaaactt ccccagaaga caccgacgat cgaacagccc ttgagcagcg
     7741 gcaggttgag cgggatgctc gggatctgcc ccgcggcgaa cccgatgacg aggtagcgcc
     7801 cgccccaggc catgctgcgc agcgccggct cggcgagatc gccgccgacg ggatcgtaga
     7861 cgacgtccac gccctttccg cccgtgatct ccttcaggcg ctggcgcagg tcctcggtcg
     7921 aataattgac caggtgcgca gcgccgaggg agcgggccag ttcgagcttc tcggcagtgc
     7981 tggccgcggc gatcaccgtc gcgcccatct cccggcccag ctcgacggcg gtcgttccga
     8041 cgccaccggc ggcgcccagc accagcaggg tctcgccggg cgccagcgac gcccgctgct
     8101 tcaaggcgtg cagcgtcgtg aaatacgtga tgcagacgcc ggccgctgcc tcgaacgaca
     8161 ggccgggcgg cgccgggacg acgagcgacg cgcgcgcggc gacacgctct gccagggtcc
     8221 ttccgcacgc ggcgacgacg cgatcgccga ctcgcaggtg cttcacgccc tcgcccaccg
     8281 agctcacgac ccccgacatc tccgaaccga cgacgaacgg cagcgggggc ctttcctggt
     8341 acaggccccg gatcgtgagc aggtccggga aattcagcgc cgccgccttc acgtcgacga
     8401 cgacctcgcc aggcgccgcc acgggctccg gccagtccgc ctcgagtctc aagccctcga
     8461 tgccggtcaa ttcccggcag accactgcac gcattcaacc tcccggtcgg ccgtgggccg
     8521 acatgtatcg ttcgtcgaga ccagcggcgg agccctggtc gaatcgcttg ccagccacga
     8581 tcgggctacc accgccgccg gcaatggtgc ttcaggtccg gatcaggtgc ctgcccagct
     8641 cggggatcga accgcagccg agcagcagca tcgtgcgctc catctcggtg cggaggatcc
     8701 cgatcgcgtg gcggacgccc gcctcgccgc cggccgcgag cccgtacagc gcggcacggc
     8761 cgatcatgac cccgtgcgcc cccagcgcga tcgccttcag gacgtcggag ccgcggcgga
     8821 atccgccgtc caccagcacc gtcagccggt cgcgcagttc gctcgcgacc gccggcagca
     8881 gttccacgcc cgacacgcag gtgtcgagtt gtcgtccacc gtggttcgag agcacgacgc
     8941 cgtcgcaccc tgcctcggcc gcgcggcgcg catcctcgat gcacagcacg cccttcacga
     9001 cgagcttgcc gggccagcgc gcgcggatgc gctcgacgtc gcgccacgac agccgcgaat
     9061 tgatctgcga gaagctgtac ttggcgccga tcagcgcgga gtacttgccg ggcggcagga
     9121 actccgtcag gttctcgaac gtcggcgcgc cggacgggaa catgacgcgc gcgagccagc
     9181 cgggatggct cagcacgtcg agcttgctcg ccaggctgag ctgcatcggc gcgctgtaat
     9241 tgcgctggtc ccactcgcgc gcgccgagca ccggcgcatc ggccgtgatc acgagcactt
     9301 cgcagccggc ctcgcggacg tggtcggtca gtcggtcgac cacgttctgg tccttgtagg
     9361 cgtacagctg gtaccagtga ttgccctgga ccgctgccgc cagccgcgcc accgagcagt
     9421 tcgacgccgt gctgagcgtg aacgggacgc cggcctcgcg ggccgcgctc gccagcgcga
     9481 tgtcgccctg cttccacaac aggccattga acccggtcgg cgcgatgacg atcggcaggt
     9541 tgacgggctt gccgaagatc tccgagccca tgtccggagg gcccgcgccg accagcgccc
     9601 tgggctgcca ggtgatcttc tcgaacacgt cgcggttgcg ccgcagcgtc acctcgtcct
     9661 cggcgccgcc ctcgacatac tcgtaggaga aattggggag gcgccggcgg gcgacgcgac
     9721 gcaaatcctc gatcgtgatc gccctgcgga aatcgcggcc ggcgtagtgc tgccgtttca
     9781 tgcccgtcct gccctgtcgt ggaccagttc cgtgatgcgc agcgccgtct cgagcgcgtg
     9841 cagtccgtcc tcgcccgaca cgacgggtgg cgtaccgtcg cgcacggacc tgaggaacgc
     9901 ctcgatctcg agcctgagcg catcgccctg ctcgtatccc cgctcctcga tcgacacctg
     9961 gcccggcgcc gcacgcgccc cctcgggcgg cttgcgcacg atggtgagga tcttctgctg
    10021 caggtcgatg gagatgtagg cgtcgtcctg gaacagccgc agcttgcgct cgaccttcat
    10081 gctgatgcgg cttgccgtcg tgttggcgac acagccgttc gcgtagcgga tgcgcgcgtt
    10141 ggcgatgtcg agttcgcccg agaaca
//
LOCUS       DAOMFD010000066        10068 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1905_length_10068_cov_35.179666, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000066 DAOMFD010000000
VERSION     DAOMFD010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10068)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10068
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1905_length_10068_cov_35.179666"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1654)
                     /locus_tag="PL152_05560"
     CDS             complement(<1..1654)
                     /locus_tag="PL152_05560"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HQR48779.1"
                     /translation="MKLETRDARSIPFQEASLDIWDKKYRLSAKDGTPIDQTMDDTYK
                     RVARALADVEKDEVREQWYEQFLWALRRGAIPAGRVTSNAGAQEHKPATSTINCTVSG
                     TIRDSMDDILGKVHEAGLTLKAGCGIGYEFSTLRPRGAYVSGAGAYTSGPLSFMDIYD
                     KMCFTVSSAGGRRGAQMGTFDIGHPDVMEFVRAKREAGRLRQFNLSLLITDEFMHAVR
                     EDREWQLAFPVRQAEIEGQDTSDRAKFIWREWPDRSNYVVNDEGLVLCRVYKTLPAKR
                     LWDVIMSSTYDFAEPGFVLIDRVNEMNNNWWCENIRATNPCGEQPLPPYGSCLLGSIN
                     LTSFVRDPFTEQAHFDWDEYRQVVHVFTRMLDNVVEVNGLPLEKQREEIQRKRRHGMG
                     FLGLGSTITMLGWKYGSRDAVAFTERVSKELAIAGWEAALELAGEKGPAPIMLEEFTV
                     TPEMLRKRPEMRRDGIKVGQKLPGRILHARYSRYMQRVAEVAPQLVEKLAATGARFTH
                     HSSIAPTGTISLSLANNASNGIEPSFAHHYFRNVIREGRKTKE"
     gene            2227..3567
                     /locus_tag="PL152_05565"
     CDS             2227..3567
                     /locus_tag="PL152_05565"
                     /inference="COORDINATES: protein motif:HMM:TIGR02037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HQR48780.1"
                     /translation="MKLHLALAALALTAWSAVAPAQIPAQAGETPVPSLAPMLKRVTP
                     AVVNVQTRGTVQEQNPLLNDPFFRRFFDVPNQPMEREFQAAGSGVIVDAKNGYIVTNA
                     HVIENAKEITVQLLDNRSLKAKVVGTDQGSDIAVLKVQGANLVEIPIADSDLAEVGDF
                     VVAIGNPFGLGHTVTSGIVSALGRSGINPEGYENFIQTDASINPGNSGGALVNLNGQL
                     VGINSAILSRSGGNIGIGFAIPSNMMNTVMQQLIKYGEVRRGVLGVNIQTLTPDIAQS
                     MELGSTQGALVSQVVDGSAADKAGIKAGDVITAVNGKPVKDSGSLRAMIGMLRIGDKV
                     DIALMRDGKPRRVTAVIGERDSAAEAKAAGGGGHEGLQGADLADAQGGGVLVRSVADG
                     SPAAQRGLRPNDVIIQVGRTRVANLAEFQRATQGLEAFVVTIRRGSAILVIPIR"
     gene            complement(3629..4711)
                     /locus_tag="PL152_05570"
     CDS             complement(3629..4711)
                     /locus_tag="PL152_05570"
                     /inference="COORDINATES: protein motif:HMM:NF021535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HQR48781.1"
                     /translation="MRGIVAECGASGRAWQGMAGRTARAAACVGCLSLLAGCYVMQAA
                     SGQLALMSRSRPVEKVIADPSTGPATRARLELAVAARDFAMHELELPDRGSYRKYADI
                     GREYVLWNVVATPEFSLSPLRWCFPVAGCVNYRGYFHQSDAEAFAWGLAARGHDIVVE
                     GVAAYSTLGHLSDPLLSSMLGWRDTRLAGTIFHELAHQRLYVAGDSAFNEAFASVVEE
                     EGVRRWLASRGDAAGLAAYEASLRRREEFLALLRQASDRLRDLYRAALPVDEKRVEKN
                     RVFGRLKYDYEVRRQAWGGYRGYDAWFAQPLSNASLASVATYRDCAPGLQRELAQAGS
                     LESFYARADALARLPRAERHAAVCEK"
     gene            4710..5933
                     /locus_tag="PL152_05575"
     CDS             4710..5933
                     /locus_tag="PL152_05575"
                     /EC_number="2.7.7.72"
                     /EC_number="3.1.3.-"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004810 - CCA tRNA nucleotidyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0001680 - tRNA 3'-terminal CCA addition
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional CCA addition/repair protein"
                     /protein_id="HQR48782.1"
                     /translation="MKIFLVGGAVRDRLLGRPHHEHDYVVTGATPDELVALGYRPVGK
                     DFPVFLHPTTGEQYALARTERKTGPGYYGFATRHSPDVTLEEDLARRDLTINAMAQDA
                     GGRLVDPHGGLRDLEARVLRHVSPAFVEDPLRVLRVARFAARFAPLGFTVAPETMELM
                     RRIAAAGELAALVPERVWVETERALGEERPRVYFDVLRDCGALSVVFPELAALDGVPQ
                     PEKWHPEGDAGLHTMLVLEAAAELSADTTVRFAALVHDVGKGLTPREQWPRHIGHEES
                     GARLIDAMSRRLRIPGEHHDLAVLVARHHAKVHRAAELRPGTLLELLEATDAFRRAER
                     FERLVVACEADARGRGPELHAAPYPQSALVRRAREAAAAVRLNPEAMADAAGPIIAER
                     LRAARIEAIRVALAG"
     gene            complement(5962..7140)
                     /locus_tag="PL152_05580"
     CDS             complement(5962..7140)
                     /locus_tag="PL152_05580"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQR48783.1"
                     /translation="MSAAAGPPDLTADEAAHGERLTALIREEIARAGGWISFARYMDL
                     ALYAPGLGYYSAGARKLGPAGDFVTAPEVAPVFSRCLADQCVEVLRRLGGGDVLEFGA
                     GSGAMAAAMLSEFESQAVLPGRYFILDVSADLRERQRATIEAAVPHLLDRVEWLDRLP
                     DCIEGVIVANEVLDAMPVERCTMRAGEVRALGVGVQPQGFAWAERAAGPALRDAVLRI
                     QAECGLDWPEGYTSEVNLGLSPWLASVAASLGRGVMIFIDYGLPRREYYAAERAAGTL
                     LCHFRHRFNDEPLARTGLQDITAWVDFTAVAEAAEAAGLQVAGYTTQAHFLIGNDLGR
                     RMQDVDSLDLAQRLNLSRQAMVLTLPGEMGERFKVIALAKGYEAPLRGFAVRDLRHTL
                     "
     gene            7194..7940
                     /locus_tag="PL152_05585"
     CDS             7194..7940
                     /locus_tag="PL152_05585"
                     /EC_number="1.5.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF006598.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pteridine reductase"
                     /protein_id="HQR48784.1"
                     /translation="MHATDALAGRCVLVTGGAKRLGAAMARRFHAAGASVVVHYHRSR
                     PAADELVAELNGARAGSAFAACADLQDVARLPGLVERALDRCGRLDVLVNNASTFYPT
                     PLGTITPAQFDDLLGTNLRAPLFLSQAAAPALRVTQGLILNMVDIHGRRPLKGHPVYS
                     AAKAALVMLTKSLARELGPDIRVNGIAPGPVLWPERDLDATLKEEIIAKTALKRSGSP
                     EDIARTALYLAADAPYVTGQVITVDGGRSL"
     gene            complement(7949..>8252)
                     /locus_tag="PL152_05590"
     CDS             complement(7949..>8252)
                     /locus_tag="PL152_05590"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQR48785.1"
                     /translation="RSRDAPRWAPRSMDLDILVYGSLVVDAQGKRGLTQKHSLTLPRP
                     DLTRRAYMLGPAADLAPDFVHPLLGATLGELWDRFDQRLHPLHEVDLGWRPQVRAG"
     assembly_gap    8253..8352
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8353..9137)
                     /gene="tsaD"
                     /locus_tag="PL152_05595"
     CDS             complement(<8353..9137)
                     /gene="tsaD"
                     /locus_tag="PL152_05595"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HQR48786.1"
                     /translation="MAVYDGERGLLAHEIFSQVDLHAAYGGVVPELASRDHLRKLLPL
                     LKKTVEAGGGRAAVDGIACTVGPGLAGALLVGSSVARSLAYAWRVPCVGVHHMEGHLL
                     APLLEPHPPAFPFVALLVSGGHTLLAHVRGVGRYELLGSSLDDAAGEAFDKTAKLLGL
                     GYPGGPQLAALAERGRSGAFEFPRPMLDRPGLDFSFSGLKTAVVVALRGRVLNDQTRA
                     DAALAFEEAVVETLVTKAMRAVEATDAQVLVVAGGVGANHRLRA"
     gene            9290..9508
                     /gene="rpsU"
                     /locus_tag="PL152_05600"
     CDS             9290..9508
                     /gene="rpsU"
                     /locus_tag="PL152_05600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011235429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HQR48787.1"
                     /translation="MPSVRVRENEYFDAALRRFKRACEKAGVLTELRRREFYEKPTQE
                     RKRKKAAAVKRHQKRGMRDYSAPRARSF"
     gene            9522..9968
                     /locus_tag="PL152_05605"
     CDS             9522..9968
                     /locus_tag="PL152_05605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HQR48788.1"
                     /translation="MTLKERITEDMKAAMRASDKERLSTIRMAQAAIKQREVDERITL
                     DDSQVISVLEKMIKQRRESVVQFEQGGRADLADKEKAEIALLQAYLPAQLSDAEIDAI
                     IREAIAATGAASMKDMGKVMGAVKAKAAGRADMGAVSTRIKAALGG"
BASE COUNT         1592 a         3563 c         3347 g         1466 t
ORIGIN      
        1 gctccttggt cttgcggccc tcgcggatca cgttgcggaa gtagtggtgc gcgaaggatg
       61 gctcgatgcc gttcgacgca ttgttcgcga gcgagagcga gatcgttccc gtgggcgcga
      121 tcgagctgtg gtgcgtgaag cgcgcacccg tcgcggcgag cttttcgacg agctgcggcg
      181 cgacctccgc gacccgctgc atgtagcggc tgtagcgcgc gtgcaggatg cggccgggca
      241 gcttctggcc gaccttgatg ccgtcgcgcc tcatctccgg gcgcttgcgc agcatctccg
      301 gggtgaccgt gaattcctcg agcatgatcg gcgccgggcc cttctcgccg gcgagctcga
      361 gcgccgcttc ccagccggcg atggcgagtt ccttcgacac gcgctcggtg aacgcgaccg
      421 cgtcgcgaga gccgtacttc cagccgagca tggtgatggt cgagcccagg cccaggaagc
      481 ccatgccgtg ccggcgcttg cgctggatct cctcgcgctg cttctcgagc ggcagcccgt
      541 tcacctcgac gacgttgtcg agcatgcgcg tgaacacgtg cacgacctgc cgatactcgt
      601 cccagtcgaa gtgcgcctgc tccgtgaacg ggtcgcgcac gaagctggtc aggttgatcg
      661 agccgagcag gcacgaaccg tacggcggca gcggctgctc gccgcaggga ttggtggcgc
      721 ggatgttctc gcaccaccag ttgttgttca tctcgttgac gcggtcgatc agcacgaagc
      781 ccggttcggc gaagtcatag gtggaggaca tgatcacgtc ccacagccgc ttcgccggca
      841 gggtcttgta gaccctgcac agcaccagcc cctcgtcgtt cacgacgtag ttcgagcggt
      901 ccggccactc ccgccagatg aacttcgcgc ggtcggaggt gtcctgcccc tcgatctccg
      961 cctggcgcac cgggaaggcg agctgccact cgcggtcctc gcgcacggcg tgcatgaact
     1021 cgtcggtgat gagcagcgac aggttgaact gccgcagccg cccggcctcg cgcttggcac
     1081 ggacgaattc catgacgtcg gggtggccga tgtcgaacgt gcccatctgc gcgccgcgac
     1141 ggccgccggc cgacgacacg gtgaagcaca tcttgtcgta gatatccatg aacgacagcg
     1201 ggcccgaggt gtaggcgccc gcgcccgaca cgtacgcgcc gcgcggacgc agcgtcgaga
     1261 attcgtagcc gatgccgcag cccgccttga gcgtgagccc cgcctcgtgc accttgccga
     1321 ggatgtcgtc catcgaatcc cggatcgtgc cggagacggt gcagttgatg gtcgaggtcg
     1381 caggcttgtg ctcctgcgcg ccggcgttgg aggtcacgcg gccggccggg atcgccccgc
     1441 ggcgcaacgc ccagaggaac tgctcgtacc actgctcgcg gacctcgtcc ttctcgacgt
     1501 cggccagcgc gcgggcgacg cgtttgtagg tgtcgtccat cgtctggtcg atgggcgtcc
     1561 cgtccttggc gctcaggcgg tatttcttgt cccagatgtc gagtgacgct tcctggaagg
     1621 gaatgctccg cgcgtcccgg gtctcgagtt tcacggcagt gctcattgcg gcgactaatg
     1681 ctccgaaaga ttcttatgcg gcttgtggct ggacaccccc ctcgggttcc cgcagcgcct
     1741 ttttcatgag gctcgtcggg cccgaccgac cccctgctcc ggcccggctc ggatcgccgt
     1801 ccctgcgcgg gacgaccgat cgtgccaaac acaacatgta gggtcaggcg ggcaagcgta
     1861 tgcaccgacc cgccccccgt caaccgcttt ttctgcggcg gcgcagcaat gtttttcctt
     1921 tacagaacat gaccttacga gcaacacccg aaagtcttgt cacatgcctg aaatgcgtcc
     1981 gccgtcaagt ccccggctgc gtcgcggaca caacatgttg tgtccacgca ctcgtcaccg
     2041 gaaatccaca gcttgcgcac cgtgggctca caggcgcaac cggcacctgt cggcagcgca
     2101 tggaactcga cgctgcgccg cccggtctca ctcgcaagtc gacgagcgcg gccgtagccc
     2161 atgggaacgg cctgctagac tcgctgcccc gcccgatccg ccccgctgaa ttcgcaggaa
     2221 tcccgcatga aactgcacct agccctcgcc gcgctcgcgc tcactgcctg gtccgccgtg
     2281 gcgccggcgc agatccccgc acaggccggc gagacgcccg tcccgtcgct cgccccgatg
     2341 ctcaagcgag tgacgccggc cgtggtcaac gtccagacgc gcggcaccgt gcaggaacag
     2401 aacccgctgc tcaacgaccc gttcttccgc cgcttcttcg acgtgccgaa ccagccgatg
     2461 gagcgcgagt tccaggcggc gggctcgggc gtgatcgtgg atgcgaagaa cggctacatc
     2521 gtcaccaacg cgcacgtgat cgagaacgcc aaggagatca cggtgcagct gctcgacaac
     2581 cgctcgctca aggcgaaggt ggtgggcacg gaccagggct ccgacatcgc ggtgctcaag
     2641 gtgcagggcg cgaacctcgt cgagatcccg atcgccgatt ctgacctcgc cgaggtcggc
     2701 gacttcgtgg tcgcgatcgg caatccgttc ggcctcggcc acacggtgac gtcgggcatc
     2761 gtgagcgcgc tcggccgctc cggcatcaat cccgagggct acgagaactt catccagacc
     2821 gacgcgtcga tcaaccccgg caactccggc ggcgcgctcg tcaacctgaa cggccagctg
     2881 gtcggcatca actcggcgat cctgtcgcgc agcggcggca acatcggcat cggctttgca
     2941 atcccgtcga acatgatgaa cacggtcatg cagcagctca tcaagtacgg cgaggtgcgg
     3001 cgtggcgtgc tcggcgtcaa catccagacg ctcacgccgg acatcgcaca gagcatggag
     3061 ctcggcagca cgcagggcgc gctcgtgtcg caggtcgtgg acggctcggc cgcggacaag
     3121 gccgggatca aggccggcga cgtcatcacg gcggtcaacg gcaagccggt gaaggactcg
     3181 ggctcgctgc gcgcgatgat cggcatgctg cgcatcggcg acaaggtcga catcgcgctg
     3241 atgcgcgacg gcaagccgcg gcgcgtcacg gcggtgatcg gcgagcgcga ctcggctgcg
     3301 gaggccaagg ccgcgggcgg cggcgggcac gaaggactgc agggcgcgga cctcgcggac
     3361 gcccagggag gcggcgtgct ggtgcgctcg gtcgccgacg gcagcccggc ggcgcaacgc
     3421 ggcctgcgcc cgaacgacgt gatcatccag gtcggtcgca cgcgcgtcgc gaacctggcg
     3481 gagttccagc gcgccacaca gggactcgag gcgttcgtcg tcacgatccg ccgcggcagc
     3541 gcgatcctgg tgatccccat ccgctgatgg aaatagggga cattctccta tttccgcagc
     3601 gcggaaatag gagaatgtcc cctatttcct atttctcgca gacggcggcg tgccgctcgg
     3661 cacgcggcag gcgcgccagc gcatcggcgc gggcgtagaa cgactcgagc gatcctgcct
     3721 gtgcgagttc gcgctgcaat cccggcgcgc agtcccggta cgtcgcgacg ctcgcgaggc
     3781 tggcattgct gagcggctgc gcgaaccacg cgtcgtagcc gcgatatccg ccccacgcct
     3841 gcctgcgcac ctcgtagtcg tacttgagcc gcccgaacac ccggttcttc tcgacacgct
     3901 tctcatccac cggcagcgcg gcacggtaga gatcccggag ccggtcggac gcctgccgca
     3961 gcagcgcgag gaattcctcg cgcctgcgca gcgatgcctc gtacgccgcg agccccgccg
     4021 catcgccgcg tgaggccagc caacgccgca cgccctcctc ctcgacgaca ctcgcgaacg
     4081 cctcgttgaa tgcgctgtcc cccgcgacgt acaggcgctg gtgcgcgagc tcgtggaaga
     4141 tcgtcccggc gagacgcgtg tcgcgccagc cgagcatgct gctcaacagg ggatcggaca
     4201 ggtgaccgag cgtcgagtaa gccgcaacgc cctcgacgac gatgtcatgg ccgcgggccg
     4261 cgagccccca cgcgaacgct tcggcgtccg actggtgaaa gtagccgcga tagttcacgc
     4321 agccggccac cggaaagcac caccgcagcg gactcagcga gaactccggc gtcgccacga
     4381 cattccacag cacgtactcg cggccgatgt cggcgtactt gcggtagctg ccgcggtccg
     4441 gcagctcgag ttcgtgcatc gcgaaatcgc gcgccgcgac cgcgagctcg agtcgcgcgc
     4501 gcgtggccgg gcccgtcgac gggtccgcga tgactttctc gacggggcgg ctccgcgaca
     4561 tgagcgcgag ctgtccgctc gcagcctgca tgacgtagca gccggccagc aacgacaggc
     4621 aaccgacgca ggcggccgca cgcgccgtcc gcccggccat cccctgccag gctcgcccgc
     4681 tcgcgccgca ttccgctacg atgccccgca tgaagatctt cctggtcggc ggcgcggtcc
     4741 gcgatcgcct gctcggccgc ccgcaccacg agcacgacta cgtcgtcacc ggcgccacgc
     4801 ccgacgagct cgtcgcgctc ggctaccggc ccgtgggcaa ggacttcccg gtcttcctgc
     4861 acccgacgac cggcgaacag tacgcgctcg cacgcaccga acggaagacc ggcccgggtt
     4921 actacggttt cgcgacgcgc cattcgcccg acgtgacgct cgaggaggac ctcgcccgcc
     4981 gcgacctcac catcaacgcg atggcgcagg atgccggcgg caggctcgtc gatccgcacg
     5041 gcggcctgcg cgacctcgag gcccgggtgc tgcgccacgt ctcccccgcc ttcgtcgagg
     5101 acccgctgcg cgtgctgcgc gtcgcgcgct tcgccgcgcg cttcgcaccg ctcggcttca
     5161 cggtcgcgcc ggagaccatg gaactcatgc gtcgcatcgc cgccgccggc gagctcgcgg
     5221 ccctggtgcc ggagcgcgtg tgggtcgaga cggagcgcgc gctcggcgag gagcgtccgc
     5281 gagtgtattt cgacgtgctg cgcgactgcg gtgcgctctc ggtggtgttc ccggagctgg
     5341 cagcgctcga cggcgtgccg cagcccgaga agtggcaccc ggaaggcgat gccggattgc
     5401 acacgatgct cgtgctcgag gcagcggcgg agttgtcggc cgacacgacg gtgcgcttcg
     5461 ccgccctggt gcacgacgtg ggcaagggcc tcacgccccg cgagcagtgg ccgcgacaca
     5521 tcggccacga ggagagtggc gcacgtctga tcgatgcgat gagccggcgc ctgcggatcc
     5581 caggcgaaca ccacgatctc gcggtgctgg tcgcgcgtca tcatgcaaag gtgcaccgcg
     5641 ccgccgaact gcggcccggc acgctgctcg aactgctcga ggccaccgac gcgttccgcc
     5701 gggccgagcg cttcgagcgt ctcgtcgtcg cgtgcgaagc cgatgcgcgc gggcgcgggc
     5761 ccgaactgca cgccgcgccc tatccgcagt ctgcgctggt ccgccgtgcg cgcgaggccg
     5821 cggctgccgt gcgcctgaat cctgaagcca tggcagacgc ggctgggccg atcatcgcgg
     5881 agcggctcag ggccgcccgc atcgaggcga tccgcgtagc gctggccggc tgagccggcc
     5941 cgtcaccgga cgattggcca cctacagcgt gtggcggaga tcgcgcacgg caaagccgcg
     6001 caggggcgct tcgtaaccct tcgcgagcgc gatcaccttg aatcgctcgc ccatttcgcc
     6061 cgggagcgtg agcaccatcg cctggcgcga caggttcagg cgctgcgcca ggtcgagcga
     6121 gtccacgtcc tgcatgcgcc gcccgaggtc gttgccgatc aggaagtggg cctgggtcgt
     6181 gtaaccggca acctggaggc ccgcggcctc cgccgcctcg gcgaccgccg tgaagtccac
     6241 ccacgccgtg atgtcctgca gcccggtgcg cgcgagcggt tcgtcgttga accggtggcg
     6301 gaaatggcag agcagcgtgc cggccgcgcg ttccgccgcg tagtactcgc ggcgcggcag
     6361 gccgtagtcg atgaagatca tcacgccccg gccgagcgag gctgcaacgg acgccagcca
     6421 tggcgacagc ccgaggttga cctcggaggt gtagccctcg ggccagtcga gcccgcattc
     6481 ggcctgtatg cgcagcaccg cgtcgcgcaa tgcagggccg gcagcgcgct cggcccaggc
     6541 gaacccctgc ggctgcacgc cgacgccgag ggcacggacc tcgcccgcac gcatcgtgca
     6601 gcgctcgacc ggcatggcgt ccagcacctc gttcgccacg atcacgccct cgatgcagtc
     6661 gggcaggcga tcgagccact cgacgcggtc gagcaggtgc ggaacggcag cctcgatggt
     6721 ggcgcgctgg cgctcgcgca ggtccgcgct cacgtcgagg atgaagtaac ggcccggcag
     6781 caccgcctgg gactcgaact cggagagcat cgcggccgcc atcgcccccg agcccgcgcc
     6841 gaattcgagc acgtcaccgc cgcccagtcg gcgcaggacc tccacgcact ggtcggcgag
     6901 gcagcggctg aacactggcg ccacctcggg cgcggtcacg aaatctccgg ccgggcccag
     6961 cttgcgcgca ccggcgctgt agtagccgag gcccggcgcg tagagagcca ggtccatgta
     7021 tcgagcgaag ctgatccagc cgcccgcgcg ggcgatctcc tcgcggatca aagccgtgag
     7081 gcgctcgccg tgcgccgctt cgtccgcggt gaggtctggc ggcccggcgg cggcgctcat
     7141 gcacgcgccc ccggcgacgc gacagcgcgg gcggtctccg gtatcgtcgg atcatgcatg
     7201 cgacagacgc actggctggc cgctgcgtgc tggtcaccgg cggcgcgaag cggctcggcg
     7261 ccgcgatggc acggcggttc catgccgccg gcgccagcgt cgtggtgcac taccaccgtt
     7321 cccgtccggc cgccgacgaa ctcgtggcgg aactcaatgg cgcacgagca ggctccgcgt
     7381 tcgcggcctg cgccgacctg caggacgtcg cgcgactgcc gggactcgtg gaacgcgcgc
     7441 tcgaccgctg cggccggctc gacgtgctgg tgaacaatgc gtcgacgttc taccccaccc
     7501 cgctgggcac gatcacgccg gcacagttcg acgacctcct cggcaccaac ctgcgcgcgc
     7561 cgctgttcct ctcgcaggcc gccgcgcccg ccctgcgcgt gacgcaggga ctgatcctga
     7621 acatggtcga catccacggc cgccgcccgc tcaagggtca cccggtctat tcggccgcga
     7681 aggccgcgct cgtcatgctg accaagtcgc tggcgcgcga actcggtccc gacatccgcg
     7741 tgaacggcat cgccccggga ccggtgctct ggccggagcg cgacctggac gcgacgctca
     7801 aggaggagat catcgcgaag acggcgctca agcgcagcgg ttcgcccgag gacatcgccc
     7861 gcacggcgct ctacctcgcg gccgatgcgc cgtacgtcac gggccaggtg atcaccgtgg
     7921 acggcggtcg cagcctctga cacgcggctc atccggcacg tacctgcggt ctccagccga
     7981 ggtcaacctc gtgcagcggg tgcagccgct ggtcgaatcg atcccacagt tcgcccaggg
     8041 tcgcgccgag caacggatgc acgaagtccg gcgcgagatc cgccgctggc ccgagcatgt
     8101 aggcccggcg cgtgaggtcc ggtcgcggca gggtcaaact gtgtttttgg gtcagacccc
     8161 gtttcccctg tgcgtccacg accagcgatc catacacgag gatgtcgagg tccatcgaac
     8221 gcggcgccca tcgaggggca tcgcgtgagc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8341 nnnnnnnnnn nngcccgcag ccggtggttg gcgccgacgc caccggcgac caccaggacc
     8401 tgcgcatcgg tcgcctccac ggcgcgcatc gccttggtca ccagtgtctc gaccacggcc
     8461 tcctcgaatg cgagggcggc gtcggcgcgg gtctggtcat tcagcacgcg tcccctcagc
     8521 gccacgacca ccgcggtctt gagaccgctg aagctgaagt cgagccccgg acgatcgagc
     8581 atcgggcgcg gaaactcgaa tgcgcccgac cgtccgcgtt cggccagcgc ggcgagctgc
     8641 gggccacccg gataccccag cccgaggagc ttggcggtct tgtcgaaggc ctcgccggcc
     8701 gcgtcgtcga gcgagctgcc gagcagttcg tagcgcccga cgccccggac gtgcgcgagc
     8761 agcgtatggc cgcccgatac cagcagtgcc acgaacggaa atgccggcgg gtgtggctcg
     8821 agcaacggtg ccagcaggtg gccttccatg tgatgcacgc cgacgcaggg cacgcgccac
     8881 gcgtaggcca ggctgcgcgc gaccgacgag ccgaccagca gcgccccggc cagccccgga
     8941 ccgacggtgc aggcgatgcc gtcgacggca gcccggccac cacccgcctc gaccgttttt
     9001 ttcagcaggg gcaataactt gcgaaggtgg tcacgtgacg ccagctccgg caccacgcca
     9061 ccgtatgcgg cgtgcaggtc gacctggctg aagatctcgt gcgcaaggag gccccgctcg
     9121 ccgtcataaa ccgccacggc ggtctcatca caggacgtct cgaggcccag cacccgcatg
     9181 ttttgcaatt cctgcccacg ctctctagaa ttcgcggtcc tcttacccgg cagagcgacc
     9241 ccttgggtcc gggccgggcg atccgaccag accgactgac gaggtttcga tgcccagcgt
     9301 ccgcgttcgt gagaacgaat acttcgatgc cgccctgcgc cggttcaagc gcgcctgcga
     9361 gaaggccggc gtcctgaccg agcttcggcg tcgcgaattc tacgagaagc cgacccagga
     9421 gcggaaaaga aagaaggccg ccgcggtcaa gcgccaccag aagcgcggca tgcgcgacta
     9481 ctcggcgccg cgcgcacgca gcttctgatc ccgccgtccc agtgacgctc aaggagcgca
     9541 tcaccgagga catgaaggcc gccatgcggg cctccgacaa ggagcgcctc tcgaccatcc
     9601 gcatggccca ggccgcgatc aagcagcgcg aggtcgacga gcgcatcacc ctcgacgact
     9661 cgcaggtgat ctcggtcctc gagaagatga tcaagcagcg gcgggaatcg gtggtgcagt
     9721 tcgagcaggg cggccgcgcc gacctggccg acaaggaaaa ggccgagatc gccctgctgc
     9781 aggcctacct cccggcccag ctctccgacg ccgagatcga cgcaatcatc cgcgaggcga
     9841 tcgccgcgac cggcgcagcg tcgatgaagg acatgggcaa ggtcatgggc gcggtgaagg
     9901 ccaaggccgc gggccgtgcc gacatgggcg ccgtgagcac ccgcatcaag gccgccctcg
     9961 gcggctgagc ggtcgctcct cgcggactca aacgggcgac tcccgggcgg ctaccgggtc
    10021 ctgtccggac tcgcgaagaa aatatgctcg cccggacagg acccgata
//
LOCUS       DAOMFD010000067        10044 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1916_length_10044_cov_27.378917, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000067 DAOMFD010000000
VERSION     DAOMFD010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 10044)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10044)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10044
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1916_length_10044_cov_27.378917"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PL152_05610"
     CDS             <1..309
                     /locus_tag="PL152_05610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HQR48789.1"
                     /translation="KRPYITAGFAGLVMLVPLAITSTNGWMRRLGRRWHALHRLVYPA
                     AILGVVHYWWQVKADWREPLLYAIVLAALLGWRVRRAWLRRAASARAPVGVPSRSPAA
                     "
     gene            complement(289..1179)
                     /locus_tag="PL152_05615"
     CDS             complement(289..1179)
                     /locus_tag="PL152_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR48790.1"
                     /translation="MSTTPHVRRALLQIHFCVVLWGFTAILGKLISLPALQLVWWRML
                     IVVAAIALWPPVWRAVRETDARMLLRFAGAGVVVALHWLTFYGAIKLSNASVAATCMA
                     MAPIMMAIVEPWIARRAFDPRELLIGVVALPGVALVVGGTPGGMRTGIVVGAISALLV
                     VVFSSLNKRYVERAGPLVMTWVELAAGTAFLTIVAPLMPGPTAAFVVPDARDFALLLT
                     LAIACTLLPFTVSLHALRHTSAFTAQLAISLEPVYAVILAIVLLGEQRELSLSFYIGV
                     ATIVGAVVAHTMLKLRGGAA"
     gene            complement(1228..2307)
                     /locus_tag="PL152_05620"
     CDS             complement(1228..2307)
                     /locus_tag="PL152_05620"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HQR48791.1"
                     /translation="MTYAAITGWGKCLPPAVLTNADLATIFDTSDEWIVSRTGIRERR
                     VSHVTLSDMAYVAAMRALAAAGLDGSQIDLIIMGTCSFDDQVPNQASGLQVRLGAKQA
                     ASMDVNTACTSFLYALSTATGLIRTGVIRNALVVGGELISAFMDWTNRGVAVLFGDGC
                     AAVVVEATDKEEGLLAAKLGCDSDARNTLVVEGMGTRYANYTRMYGDTNWIFEGQEIF
                     KRAVLGMSRACADVLENRHLSADDIDLVIPHQANLRIIEAVAKRAGMPMERVFVNVHR
                     YGNMSAATVPVALVEALEEGRVQPGSLLLMPAFGAGLTWCAHLVRWGDRVTPKAVSDV
                     DLPPCDMTALQIVENLRRRKAAAAR"
     assembly_gap    2348..2447
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2448..3187)
                     /locus_tag="PL152_05625"
     CDS             complement(<2448..3187)
                     /locus_tag="PL152_05625"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HQR48792.1"
                     /translation="MPLDRHVEIMEVGPRDGLQSEPEVLPTGTKVEFIARAIAAGVRR
                     IEVASFVNPKRVPQMADAEDVLKALPRRGDVRYVGLVLNLRGFERAAAAGCDEVGMAV
                     AASDTFNRRNQGVPTQDSLSAWAAIAAAARQAGLRAQVTISTAFGCPFEGEVPVERVV
                     EIAKRVADAGPFEISLADTIGVGVPAQVEDVVGRVREAVPGARLRGHFHNTRNTGLAN
                     AYAAVRCGVTALDSSLGGIGGCPFAPGAT"
     gene            3382..>4029
                     /locus_tag="PL152_05630"
     CDS             3382..>4029
                     /locus_tag="PL152_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48793.1"
                     /translation="MKKANLVLMAAIAAAGVVGVAAGALVPVVAVAAETQKVSASVAK
                     PLKAAQDAMAAKNWDEALVHIQEAQAVQPQSAYDTFMIDEFGWYVQLQKKDYAAAATM
                     LEASVNSGFLPADKMQDRLKALTQINYQMLKNYAKAIEFGNRYLQAAPNDQDVGPMVA
                     ASYYQLKDYAAARSAAQKVAAASAKPTEAMLQIVMFSSASLNDRPATMQALEQLVR"
     assembly_gap    4030..4129
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4130..4477
                     /locus_tag="PL152_05635"
     CDS             <4130..4477
                     /locus_tag="PL152_05635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48794.1"
                     /translation="DAKDLPNADKQLAAAKSGNEMLAIGKLYFSAGDYAKAADAIGKG
                     LAKGGVTDTDDANALLGIAHVRAGNAADARTAFGAVKDPKYATVARLWLLYLDSTSPA
                     AAPTATGATPPPG"
     gene            complement(4583..5800)
                     /locus_tag="PL152_05640"
     CDS             complement(4583..5800)
                     /locus_tag="PL152_05640"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR48795.1"
                     /translation="MRTARFVLSAALAAGSVVGVTAALLVPATAMAEQKVGATVGKYL
                     KTAQEAMQKKKWDQALAAIKQAQAVDTKTPFEQYKINELLWYVYLQQGRNLDAARLLE
                     EQMNSGMMPAGEKVERTKTLAQLYFRAGNYGRAIQYANQYLKSAPGSQDMQLLVAQSY
                     YQQKDYKNAIAAADRMTKGGGRPSEDVLQLMLRSSYEINDKEGTRKALELLLKYYPSP
                     DTWARLLDGYIAQTKHDDELLSLYRLAEDVGALNKARQYTDMSQGLVVAGFAIEGQRI
                     IEQGLAASVFQGEELSRAQRTLESAKRRADAERQQLPKAAAALAAAATGPQAFAVGKL
                     YFSAADYPNAVLALNKAIAKGGLPDADSAENLLGIALARQGKAADAKKAFLGIKDPKF
                     EEVARLWIIKVGG"
     gene            complement(5920..6336)
                     /locus_tag="PL152_05645"
     CDS             complement(5920..6336)
                     /locus_tag="PL152_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR48796.1"
                     /translation="MGMSTGGGEDEPMMDINTTPLIDILLVLLIMFIITIPVMTHAVK
                     LDMPRANNAPSQAQPEVINLEIDFDGTILWNGTPVPDLDTLDQYFRGEAAKDPQPELH
                     IRPSKRVDYDVVAKVLAGAQRNGLLKIGFVGNEQFM"
     gene            complement(6365..6802)
                     /locus_tag="PL152_05650"
     CDS             complement(6365..6802)
                     /locus_tag="PL152_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR48797.1"
                     /translation="MSVTTGGGPSGDEKPMSEINTTPLVDVMLVLLIIFLITIPVITQ
                     SVKVNLPRAANIPTQTKPENINIAVDADGNVYWNTALVPTQEALLAKLKGVAVMDPQP
                     EVHLRGDRQTKWENIGRVVVLCQRSGIVKVGFITEPDRGLARM"
     gene            complement(6839..7261)
                     /locus_tag="PL152_05655"
     CDS             complement(6839..7261)
                     /locus_tag="PL152_05655"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR48798.1"
                     /translation="MTESVPLHEWIDNVIARGVARIGNALSTQLPFLATTGSTAPFIG
                     LFGTVWGIYHALVAIGVAGQASIDKVAGPVGEALIMTAIGLAVAVPAVLGYNYLLGRN
                     KEIMEKVKLFSADLEQVIVSGSRVEGSKARVISGGPKK"
     gene            complement(7637..8293)
                     /locus_tag="PL152_05660"
     CDS             complement(7637..8293)
                     /locus_tag="PL152_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR48799.1"
                     /translation="MAYDYSIDDPAKNSKRTLAFVLVVIIHVLFFWVLASGLGAKVVE
                     VVMGPVETKIIEEKPEEEKPPPPPPQLEQPPPYVPPPEVSIEIPVETGPSTAITNTTS
                     ERPVAAPVAPAAPVAPAHQVVKTLPSTQGKGARITQPDYPPASRRAGEAGTVTLAAYV
                     LETGKVGEIKIVKSSGFDKLDQAAVQEVQRNWRFVPGKEDGKPVAMWHTFKITFQLKD
                     "
     gene            complement(8468..>10044)
                     /gene="ilvD"
                     /locus_tag="PL152_05665"
     CDS             complement(8468..>10044)
                     /gene="ilvD"
                     /locus_tag="PL152_05665"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF002068.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009099 - valine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HQR48800.1"
                     /translation="IASTWSNLTPCNMHIDKLAAAAARGADAVGGKSTVFGTITVSDG
                     ISMGTPGMRYSLVSREVIADSIETVAGAQGFDGLVAIGGCDKNMPGCMMAIARLDRPA
                     VFVYGGTIRPGAQRRDIVSVFEAVGAHAQGAISDRQLREVECTAIPGPGSCGGMYTAN
                     TMASAIEALGMSLANSSAQEAVSRHKRQDAEQAGRAVVDLIRRGIKPSDILTREAFEN
                     AIVVTIALAGSTNAVLHLLAIAHAAGVPLVLDDFTRVGAKVPVLADLRPSGRYSVSEL
                     IRIGGIQPLMKELLGAGLLHGGCLTVTGKTLAQNLRRVKPYPAGQDIVRPLANPIKPE
                     SHLVVLYGNLAPEGAVAKITGKEGLRFSGRARVFDGEEATLQAILDGTVVAGDVLVVR
                     YEGPRGGPGMREMLSPTAAIMGKGLGDKVALITDGRFSGGSHGFVVGHVSPEAATGGP
                     LALVRDGDSIIIDATARTLTVALSNDELSRRRAQWVAPKPYARRGVLAKYAELVSSAS
                     LGAVTDRDLAACRPAD"
BASE COUNT         1537 a         3407 c         3255 g         1645 t
ORIGIN      
        1 aagcgtcctt acatcacggc gggcttcgcg ggtctcgtca tgctcgttcc attggcgatc
       61 acctcgacca acggctggat gcgccgcctc ggccgtcgct ggcacgcgct gcaccggttg
      121 gtgtacccag cggccatcct gggggtcgtg cactactggt ggcaggtcaa ggccgactgg
      181 cgcgagcccc tgctgtacgc catcgtgctg gcggcgctgc tcggctggag agtgcggcgc
      241 gcgtggctgc ggcgggccgc tagtgcccgt gcgccggtcg gcgtgccctc acgcagcccc
      301 gccgcgtagc ttcagcatcg tgtgcgccac cacggcgccg acgatcgtcg cgacgccgat
      361 gtagaaagac aaggacaatt cgcgttgttc gccgagcagc acgatcgcga ggatcaccgc
      421 gtacaccggc tcgaggctga tcgcgagttg cgcggtgaac gcgctcgtgt gacgcagcgc
      481 gtgcagcgaa accgtgaatg gcagcagcgt gcaggcgatc gccagggtca gcaggagcgc
      541 gaagtcgcgc gcgtccggca cgacgaatgc ggcggtcgga cccggcatca gtggcgcgac
      601 gatcgtgagg aaagcggtgc cggctgccag ttccacccag gtcatcacga gcggcccggc
      661 cctttccacg tagcgcttgt tgagcgacga gaacaccacg acgaggagcg cagagatcgc
      721 gcccaccacg atcccggtgc gcatgccacc gggcgtgccg ccgacgacga gcgccacgcc
      781 cgggagtgcc acgaccccga tgagcagttc gcgcggatcg aatgcgcgcc tggcgatcca
      841 cggttcgacg atcgccatca tgatcggcgc catggccatg caggtcgccg cgacggacgc
      901 gttcgacagc ttgattgcgc cgtagaacgt cagccagtgc agcgccacca cgacgcccgc
      961 ccccgcgaac ctgagcagca tccgggcgtc cgtctcccgc acggcgcgcc acaccggcgg
     1021 ccacagcgcg atcgccgcga cgacgatcag cattctccac cagacgagtt gcagcgcggg
     1081 caggctgatc agcttgccca ggatggccgt gaatccccac aggacgacgc agaagtggat
     1141 ctgcagcagg gccctgcgga cgtgcggtgt tgtggacact gcgggcggtg ccagactccg
     1201 agcccccata ccgtcgaatg gatgccgtca gcgggctgcg gcggccttgc gacgacgcag
     1261 gttctcgacg atctgcagcg ccgtcatgtc gcacggcggc agatcgacgt cggagaccgc
     1321 cttgggagtg acacggtcgc cccaccgcac caggtgcgca caccaggtga gtcccgcgcc
     1381 gaatgccggc atgagcagca ggctgcccgg ttgcacccgg ccctcctcca gcgcctcgac
     1441 cagcgcgacc gggacggtgg ccgccgacat gttgccgtag cggtgcacgt tcacgaacac
     1501 gcgctccatg ggcatgcccg cgcgcttggc gacggcctcg atgatgcgca ggttcgcctg
     1561 gtgcgggatg acgaggtcga tgtcgtcggc cgacaggtgc cggttctcga gcacgtcggc
     1621 gcaggccctg gacatcccga gcacggcgcg cttgaagatc tcctggccct cgaagatcca
     1681 gttggtgtcg ccgtacatgc gcgtgtaatt cgcgtagcgc gtgcccatgc cttccacgac
     1741 cagcgtgttc cgggcatcgc tgtcgcaccc cagcttggca gccagcagtc cttcttcctt
     1801 gtctgtcgcc tccaccacga ccgccgcaca gccgtccccg aacagcacgg cgacgccgcg
     1861 gttggtccag tccatgaacg cgctgatcag ctcgccaccg acgaccagcg cgttgcggat
     1921 cacgccggtg cggatcaggc cggtggccgt gctgagggca tacaggaagc tggtgcaggc
     1981 cgtgttgacg tccatcgagg ccgcctgctt ggcgcccagc cgcacctgca acccggacgc
     2041 ctggttcggc acctggtcgt cgaagctgca ggtgcccatg atgatgaggt cgatctggga
     2101 accgtcgagg cccgctgccg ccagggcccg catcgcggcc acgtaggcca tgtcgctcaa
     2161 tgtgacgtgc gaaacgcgtc gctcacggat gccggtgcgc gaaacgatcc actcgtcgct
     2221 ggtgtcgaag atggtcgcga ggtcggcatt ggtcaggacg gccggcggca ggcacttgcc
     2281 ccagcccgtg atggcggcgt aggtcacggg acgttccccg gatcgcttgg agggcgacac
     2341 tctacccnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2401 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtc gcacccggcg
     2461 caaacgggca gccaccgata ccgcccagac tcgaatcgag tgcggtgacg ccgcaccgga
     2521 cggcggcata tgcgttggcc aggccggtat tcctcgtgtt gtggaagtgg ccgcggagcc
     2581 gcgcgcccgg caccgcctcg cgcacgcggc ccacgacgtc ctcgacctgc gccgggactc
     2641 ccacaccgat cgtgtcggcc agcgaaatct cgaacggtcc ggcatccgcg acccgcttcg
     2701 cgatttcgac cacgcgttcg acggggactt cgccttcgaa cgggcaaccg aacgccgtcg
     2761 agatggtgac ttgcgctcga aggccggcct gccgcgcggc cgcggcaatc gcggcccatg
     2821 ccgagagcga gtcctgcgtc gggacgccct ggttgcggcg attgaacgtg tcgctggcgg
     2881 caacggccat cccgacctcg tcgcagccgg ccgctgcggc gcgctcgaac cctcgcagat
     2941 tgagcacgag cccgacgtag cggacatcgc cgcgccgggg cagcgccttc aggacgtcct
     3001 cggcgtccgc catctgcggc actcgcttcg ggttcacgaa gctggcgacc tcgatcctgc
     3061 ggacccccgc ggcgatcgca cgcgcgatga actcgacctt ggtccccgtc ggcaggacct
     3121 cgggctcgct ctgcagcccg tcgcgcgggc cgacttccat gatctcgacg tggcggtcca
     3181 aaggcatgca ggcactccag gctggtccta cccctgatgg cgcacgtaac cggcgcgatc
     3241 caggccgacg caaccgctcg cgtcgggccc cgcgcctcgc gggccaggcc ggccgaacac
     3301 ggcatcccgc atgatgatgc cgtgcggctt caagatacaa tgccggccca atggcctgct
     3361 gctgggcccg cgaggcgaga catgaagaaa gcgaatctgg tgctcatggc cgcgattgcc
     3421 gcggccggcg tggttggagt ggcggccggt gcgctcgtgc ccgtggtggc cgtggccgcc
     3481 gaaacccaga aggtcagcgc aagcgtcgcc aagccgctca aggcagcgca ggacgcgatg
     3541 gccgccaaga actgggacga ggcactggtg cacatccagg aagcacaggc cgtccagccg
     3601 cagtccgctt acgacacgtt catgatcgac gaattcggct ggtacgtgca gctgcagaag
     3661 aaggactacg cggcggcagc aacgatgctg gaggcgtcgg tcaactccgg tttcctgccc
     3721 gcggacaaga tgcaggaccg cctgaaggcg ctgacccaga tcaattacca gatgctgaag
     3781 aactacgcga aggcgatcga gttcggcaac cgataccttc aggcggcgcc gaatgaccag
     3841 gacgtgggtc ccatggtcgc cgccagctac taccagctga aggattacgc cgcggcgcgc
     3901 tcggcggccc agaaggtggc cgcggcctcc gcgaagccga ccgaggccat gctgcagatc
     3961 gtcatgttca gcagcgccag cctgaacgac cggcccgcga cgatgcaggc gctcgagcag
     4021 ctggtgcgcn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng acgccaagga
     4141 cctgcccaac gcggacaagc aactggccgc ggccaagagc ggcaacgaga tgctggccat
     4201 cggcaagctc tatttcagcg ccggcgacta cgccaaggcg gcggatgcca tcggcaaggg
     4261 cctcgcgaaa ggcggcgtga ccgataccga cgatgccaac gcactgctcg gcattgccca
     4321 cgtgcgcgcg ggcaacgcag ccgatgccag gaccgcgttc ggcgccgtca aggacccgaa
     4381 gtacgccacg gtggcgcggc tttggctgct gtacctcgat tcgacgtcgc cagcggcggc
     4441 cccaacggcc accggcgcga ccccgccgcc gggctgaacg agcggcgcaa tcaatggcgc
     4501 aggaatgaga aagcccccgg ctcgcgccgg gggctttttt tcttgcggcg ctgcgccagc
     4561 gccgccggtc agcggcgtgg tctcagcctc cgaccttgat gatccacagg cgcgcgactt
     4621 cctcgaactt gggatccttg atcccgagga acgccttctt cgcgtcggct gccttgccct
     4681 ggcgcgccag ggcgatgccg agcaggttct ccgcagagtc cgcgtccggc aggccgccct
     4741 tcgcgatcgc cttgttgagc gcgagcaccg cgttcggata gtcggcggcg ctgaagtaca
     4801 gcttgcccac cgcgaacgcc tgcgggcccg tggccgccgc ggccagggcc gccgccgcct
     4861 tcggtagctg ctgccgttcc gcgtcggcac gtcgcttggc ggactcgagg gtccgctggg
     4921 cgcgcgacag ctcctcgccc tggaacacgc tggcggcgag accctgctcg atgatgcgct
     4981 ggccctcgat ggcaaagccg gccaccacga ggccctgcga catgtcggtg tactgccttg
     5041 ccttgttgag cgcacccacg tcttcggcga ggcggtacag cgaaagcagc tcgtcgtcgt
     5101 gcttggtctg tgcgatgtag ccatcgagca ggcgcgccca cgtgtccggg ctcgggtagt
     5161 acttgagcag cagctcgagc gccttgcgcg tgccttcctt gtcgttgatc tcgtaactcg
     5221 agcgcagcat gagctgcagg acgtcctcgc tcgggcgccc tccgcccttg gtcatgcggt
     5281 ccgcggcggc gatggcgttc ttgtagtcct tctgctggta gtacgactgg gcgaccagca
     5341 gctgcatgtc ctggctgccc ggcgccgact tcaggtactg gttggcgtac tggatcgccc
     5401 tgccgtaatt gccggcccgg aagtacagct gggccagcgt cttggtgcgc tcgaccttct
     5461 cgcccgccgg catcatcccc gaattcatct gttcctcgag caggcgtgcg gcgtcgaggt
     5521 tgcggccctg ctgcaggtac acgtaccaga gcagctcgtt gatcttgtac tgctcgaacg
     5581 gcgtcttggt gtcgaccgcc tgggcctgct tgatggccgc cagcgcctgg tcccacttct
     5641 tcttctgcat cgcctcctgg gcggtcttga ggtacttgcc gaccgtggca ccgaccttct
     5701 gctcggccat ggccgtcgcg ggcaccagca gggccgccgt cacgccgacc acgctgcctg
     5761 ccgcaagtgc ggcactcaat acgaatctcg ctgtcctcat cgctcgcctc actgggggcc
     5821 gtgcaccccc ttccaagaca aaagccgccg ggactcctcg tcccggcagc ctcgttgaaa
     5881 ctggcccgca ccgaccccgc aggtccgcgc cgggcttgcc tacatgaatt gctcgttccc
     5941 tacgaaaccg atcttgagca gcccgttgcg ctgcgcgccc gcgagcacct tggccacgac
     6001 gtcgtaatcc acccgcttgc tcgggcggat gtgcagctcc ggctgcggat ccttggcggc
     6061 ctcgccacgg aagtactggt cgagcgtgtc gaggtccggg accggcgtgc cgttccacag
     6121 gatcgtcccg tcgaagtcga tctcgaggtt gatcacctcg ggctgggcct gcgacggggc
     6181 gttgttggcc cgcggcatgt cgagcttgac cgcgtgcgtc atcaccggga tggtgatgat
     6241 gaacatgatc agcagcacga gcaggatgtc gatcaacggc gtcgtgttga tgtccatcat
     6301 cggctcgtct tcgccgccgc ctgtgctcat tcccatctgt ctgactcctg gtgaccgccg
     6361 ctcatcacat gcgcgcgagg ccgcggtcgg gctcggtgat gaatcccacc ttgacgatgc
     6421 cgctgcgctg gcagagcacg accaccctgc cgatgttctc ccacttggtc tgcctgtcgc
     6481 cgcgcaggtg gacctcgggc tgcggatcca tgaccgccac gcccttcagc ttggcgagca
     6541 gcgcctcctg ggtcggcacc agcgccgtgt tccagtagac gttgccgtcg gcgtcgacgg
     6601 cgatgttgat gttctcgggc ttggtctgcg tcggtatgtt ggccgccctc ggcaggttga
     6661 ccttcaccga ctgcgtgatg accgggatgg tgatcaggaa gatgatgagg agcaccagca
     6721 tcacgtccac gaggggcgtg gtgttgatct ccgacatcgg cttctcgtca cccgacgggc
     6781 caccgcccgt cgtcactgac atgcccatcg cactgcctcc ggctgctggc gcggccgatt
     6841 acttcttcgg gccgccggag atgacgcggg ccttcgaacc ctcgacgcgc gatccgctga
     6901 cgatgacctg ctcgaggtcg gcggagaaca gcttcacctt ctccatgatc tccttgttgc
     6961 ggccgagcag gtagttgtag ccgagcaccg ccggcaccgc gaccgccagg ccgatggccg
     7021 tcatgatcag cgcctcaccg accgggccgg ccaccttgtc gatgctcgcc tgaccggcca
     7081 cgccgatggc gaccagcgcg tggtagatgc cccacaccgt gccgaacagt ccgatgaacg
     7141 gcgcggtcga cccggtggtg gcgaggaacg gcagttgcgt ggacaacgcg ttgccgatgc
     7201 gggcgacgcc gcgcgcgatg acgttgtcga tccactcgtg cagcgggacg gactcggtca
     7261 tgccggacga atcgtggtgc tcggccgcgt cgatcgcttc gcctgcgatg accttgaaga
     7321 tgttgccctt gccggtcagc ttggtgacgc cggtcttgat gttgtcggca ccccagaagt
     7381 tcttctccgc ctccttggcc tggccgagca acctgttctg ctcccaccac ttggtgaaga
     7441 tgatgaacca cgagtacgcg gacatcagga ccagcaggac catgacgccc ttggcgacga
     7501 agtcgccctg cgcccagagg gcctggatac cgtacgggtt ggaaacagcg atcgattcag
     7561 cggccatggg aactttcctc caacaattcg gcttgcagtt tcttgtgaaa ttaccgggcg
     7621 gcgcggagcg cggacgctag tccttcagct gaaaggtgat cttgaacgta tgccacatgg
     7681 ccacgggctt cccgtcttcc ttgccgggca cgaagcgcca gttgcgctgg acttcctgga
     7741 cggcggcctg atccagcttg tcgaagccgc tgctcttgac gatcttgatc tcgccgacct
     7801 tgcccgtctc gagcacgtag gcggcgagcg tgacggtgcc agcctcgccg gcccggcgcg
     7861 atgccggcgg atagtccggc tgcgtgatgc gcgcgccctt gccctgcgtg gacggcagcg
     7921 tcttcacgac ctggtgcgcc ggtgccaccg gtgccgccgg cgcgaccggc gcggccacgg
     7981 ggcgctccga cgtggtgttc gtgatggcgg tcgacggccc ggtctcgacg gggatctcga
     8041 tcgacacctc gggcggcggc acgtacggcg gcggctgctc gagctgcggc ggcggcggcg
     8101 gcggcttctc ctcttccggc ttctcctcga tgatcttggt ctccaccgga cccatcacca
     8161 cttcgacgac cttggcaccg agtccggacg cgagcaccca gaagaacagc acgtggatga
     8221 tgacgacgag gacgaacgca agcgtgcgct tactgttctt ggccgggtcg tcaattgagt
     8281 agtcgtacgc cacgtcccct ccgtcgctgt ggggtctttt tcgctgtgtg tacccgggat
     8341 tcccgcggtc gtttccccga ggaagggcac actacaagag cccgtccgct gtcgcaaccc
     8401 cggcggcggg cggcgcactg tacataggca cccgggccaa ctcaataccc gggcaatcct
     8461 tgtggaatca gtccgccggc cgacatgccg cgaggtcacg atcggtgacc gcaccgagcg
     8521 acgcactgct caccagttcg gcgtacttcg ccaggacgcc gcgccgggcg tagggcttcg
     8581 gcgccaccca ctgcgcccgc ctgcgcgaca gttcgtcatt tgacagtgca accgtgaggg
     8641 tgcgagcggt ggcgtcgatg atgatcgagt cgccgtcccg gaccagggcc agcggacctc
     8701 cggtcgcggc ttcgggcgag acatgcccga cgacgaatcc atggctgccg cccgaaaagc
     8761 ggccgtccgt gatgagcgcg accttgtcgc cgaggccctt gcccatgatg gccgcggtcg
     8821 ggctcagcat ttcgcgcatg cccgggccgc cacgcggacc ttcgtaccgc acgaccagca
     8881 cgtcgccggc gaccacggtg ccgtccagga tcgcctgcaa ggtcgcctcc tcgccgtcga
     8941 acactcgtgc ccggccggag aagcgcaatc cctccttgcc ggtgatcttc gcgaccgcgc
     9001 cttccggcgc gaggttgccg tacaggacca cgaggtggct ctcgggcttg atcgggtttg
     9061 caagcggccg gacgatgtcc tggccggccg gatacggctt cacccgccgc aggttctgcg
     9121 caagtgtctt gcccgtgacc gtcaggcagc cgccgtgcag cagtcccgca ccgagcaatt
     9181 ccttcatgag cggctggatt ccgccgatgc gaatgagttc cgacaccgag tagcgaccgc
     9241 tcgggcgcag gtccgcgagc accggcacct tcgcaccgac gcgcgtgaag tcgtcgagca
     9301 cgagcggcac gcccgccgca tgggcaatcg ccagcaggtg gagaacggcg ttcgtggagc
     9361 ccgcgagcgc gatggtcacg acgatggcat tctcgaatgc ctcgcgcgtc aggatgtccg
     9421 agggcttgat gcccctgcgg atgaggtcca cgaccgcgcg ccccgcctgc tccgcatcct
     9481 ggcgcttgtg gcgggagacc gcctcctggg cggaactgtt ggcgaggctc atgcccagcg
     9541 cctcgatcgc ggacgccatg gtgttggccg tgtacatgcc gccacagctg ccggggcccg
     9601 ggatcgccgt gcactcgacc tcgcgcaact gccggtccga gatcgcgccc tgcgcatgcg
     9661 caccgaccgc ctcgaacacg gacacgatgt cgcgccgctg cgcgcccggc cggatcgtgc
     9721 cgccgtatac gaacacagcg ggtcgatcga gtcgcgcgat cgccatcatg cagccgggca
     9781 tgttcttgtc gcatccgccg atcgcgacga gtccgtcgaa gccctgcgcc ccggcgacgg
     9841 tctcgatcga gtcggcgatc acttcccgcg acacgagcga gtagcgcatt cccggcgtgc
     9901 ccatcgagat gccgtcggag accgtgatgg tgccgaacac ggtgctcttg ccgccgacgg
     9961 cgtcggcgcc gcgggccgcc gcggccgcca gcttgtcgat gtgcatgttg cacggcgtga
    10021 ggttgctcca cgtcgatgcg atcc
//
LOCUS       DAOMFD010000068         9994 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1931_length_9994_cov_32.307274, whole genome shotgun sequence.
ACCESSION   DAOMFD010000068 DAOMFD010000000
VERSION     DAOMFD010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9994)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9994
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1931_length_9994_cov_32.307274"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..863
                     /locus_tag="PL152_05670"
     CDS             <1..863
                     /locus_tag="PL152_05670"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR48801.1"
                     /translation="WREELERDYGYTSLRWLDRDETRALIASDRYIGGLLDSRSGHLH
                     PLNYTLGLARAAEAAGARLFEGSQVTRLDHGPIATVATQHGLVRARHVALCCNAYIDD
                     EVSSRLRARIMPVGTYIVATEPLGEARIRALMRENVAVTDINWVLDYFRPSADHRLLF
                     GGRVSYSGLDPLNTARATRARMVKVFPQLADARIEYSWGGYVDITMSRAPDFGRLAPN
                     VYYLQGFSGHGIALTGMAGRLVAEAIAGQAERFDLFTKLEHRDFPGGRAFRTPALVLA
                     MLWFRLKDLL"
     gene            complement(873..1235)
                     /gene="queD"
                     /locus_tag="PL152_05675"
     CDS             complement(873..1235)
                     /gene="queD"
                     /locus_tag="PL152_05675"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HQR48802.1"
                     /translation="MDIFKTFTLESAHRLPNVPAGHKCARVHGHSFRIEIHVSGAVDA
                     HTGWVMDFADIRAAFDPLYQRLDHHYLNDVPGLDNPTSENLARWIWRELKPRLAQLST
                     VVVHETCTSGARYDGSDR"
     gene            1348..2148
                     /locus_tag="PL152_05680"
     CDS             1348..2148
                     /locus_tag="PL152_05680"
                     /inference="COORDINATES: protein motif:HMM:NF025008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQR48803.1"
                     /translation="MKFGEVISTVRQAGGAWTAHIPSDWANGRTVFGGLQAALLVRGM
                     RGVLGDARALPLRSLHVTFVAPVAAGKDLVLRPELLRIGKSAAHARCDMLTGDGLVCT
                     AVAIYGADRPSAVFMEIPRVEVNTDPDQLTRWPYIPGVTPEFVKHLDIRLARGALPYS
                     GQLSAQSTLMARLVDSDCSAEEALVALSDTPPTPALSMLSKPAPAASLNWMLELLGHP
                     DDLELDAWCVIDTSVRAGSDGYLSQTSTLWGPSGHAFSVSHQSVAIFG"
     gene            complement(2212..4416)
                     /gene="rlmKL"
                     /locus_tag="PL152_05685"
     CDS             complement(2212..4416)
                     /gene="rlmKL"
                     /locus_tag="PL152_05685"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: protein motif:HMM:NF008748.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008990 - rRNA
                     (guanine-N2-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HQR48804.1"
                     /translation="MSDPPPAGTHEFFAACPRHVPDILAAELRALGIRVTREHPAGVG
                     FDGTLADGYRACLESRTASRVLLTLGTVPLDSADAMYGALKAMHWEDHLGPDGTLAVE
                     VVGEGPSWIRNTQFVAQKAKDAIADRFRERCGTRPSVDLAAPDLRVSLRLARSAATVG
                     IDLSGEPLHRRGYRQSAVEAPLKENLAAALLMRCGWPAIAAEGGAFADPMCGSGTLVI
                     EAALIAANAAPGLLRRRFGFERWLGHDASVWSELRAAAEARRSLAALGQGRLRGFDRD
                     QNAIRAALANANRAGLGDYVWFERRDVASLPQASESRGLVLVNPPYGARLEDERTLGP
                     LYGVLGRTLVEKFPGWEAGVFTGNPPLGRALGLRAYRSHTFFNGPIECRLLRFELGAD
                     AVERDVETVRRERLEAARARPGAQMFANRLAKNLSRLESWARREDVSCFRVYDADMPE
                     YAFAVDIYGNGERHACVQEYAAPPTVSRDAARGRRDEALSVLPEVLGLPADRVTLKVR
                     TRQRHGGQYEKADSEARFHVVREGRYRFLVNFTDYLDTGLFLDHRITRRRIGELAAGR
                     RFLNLFAYTGTATVYAAGGGATGSTTVDLSNTYLDWAKRNLALNDLAGPQHGFVHADC
                     LAWLDEQAATGRNRYGLVFLDPPTTSRSKRMQQSFDVQRDHAELLRTVAGLLEPGGAI
                     LFSCNYQRFRLDLESLGDFVVEDLSRATLPEDFRRNPRIHVAYLLRPRGRSD"
     gene            complement(4409..5860)
                     /locus_tag="PL152_05690"
     CDS             complement(4409..5860)
                     /locus_tag="PL152_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HQR48805.1"
                     /translation="MALTPSIGLGAPALRGRVACVDLETTGGVAAQHRIIEVGVVLLD
                     DGAVVEEWSSLVNPGQRIPRSIEQFTGISNAMVEDAPTFRDLLPDIRSRLTGRLFVAH
                     NARFDYGFLRNEFRREHEKFTAPVLCTVRLSRALFTQHARHNLDTLIERFGLECGQRH
                     RALGDAAVLPALLAAFEQSVGAGRVEEAIEAARRDARLPAHLPADLADDLPEGAGVYI
                     FRGEGGTPLYVGMSRSLRSRVLDHFATGQRASKESNLARQVREVEWVETGGELGALLL
                     ESRLVKELAPAANRRLRRASAAYCIRLDEVAGALRPRIEVVEPGDVAGDAEAYGPFRD
                     ERDAWRALEGKAREAQLCFKVLGLEQGEGSCFASQLGRCRGACAGREPLPMHDARLRL
                     ALASLRIQPWPFRGAVGIRERAPGGEGSILHVIDRWQHLGTAGDEGEIAGLLRTRDGS
                     GFDADSYRILRRCLGRVAPRDLVVLDHGTHASE"
     gene            complement(5876..6748)
                     /gene="aguB"
                     /locus_tag="PL152_05695"
     CDS             complement(5876..6748)
                     /gene="aguB"
                     /locus_tag="PL152_05695"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012537242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0050126 - N-carbamoylputrescine amidase
                     activity [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HQR48806.1"
                     /translation="MKVTLAATQFACSTEAARNLDSAERAVRAAARQGAQVILLQELF
                     ETPYFCKDHLASHFALARPIAGSPVVERFRALARELAVVLPLSVFERANNAYFNSLAM
                     IDADGSVLGVYRKSHIPEGPGYHEKFYFSPGDTGFRVWHTRYGILGVGICWDQWFPEA
                     ARCMALMGAEVLLYPTAIGSEPQDASIDSRDHWQRCMQGHAAANVMPLVASNRIGVER
                     GEKYEMTFYGSSFIAGPTGEKVVEADRSGEAVLTATFDLDAIRAQRHAWGVFRDRRPD
                     LYGPIMTLDGDGSL"
     gene            complement(6824..8446)
                     /locus_tag="PL152_05700"
     CDS             complement(6824..8446)
                     /locus_tag="PL152_05700"
                     /inference="COORDINATES: protein motif:HMM:NF017745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HQR48807.1"
                     /translation="MRHWLRMVAVAARVFPLAMLAVSGSAMASGNSYDDLLKLYEEWR
                     RFEPPPPLDGAPDYTVAAIARKSEELARYQARLAAIDPADWPVARKVDYDLVRAQMNG
                     LEFDIRWLRPWARDPAFYRSVWTEQSDTPEHEGPVNHAPVELWQYAFPLSRQDEARLT
                     RELRTVPPLLSQARRNLTGDAHDLWITGTGTMRQQDEALADLASKTRDYGPDLRDAIA
                     AAATATAEFIAWLDAQAPSKTGPSGVGKEAYDWSLRNVHLVPLTWNDQVMLLKSELAR
                     AWSSLALEEQRNRGLPQLTPAASAEEHAGRANASITKYLAFLRQRDLIEVDDYLDPAL
                     RRRIDPYVPVEQRNFFSIASHYEPVTLYAHFYHWFDHEWLVRKPNPSPIRREAWAYNA
                     WDTRAEGMATAMEEIILHAGYYDDNPRAREIVWIMLAQRCARGLASLYAQANEFDLRQ
                     AKAFQVEWTPRGWMRPDLDLLGFEQQLYLRQPGYGTSYVTGKYLIEETIKDRRRQLGE
                     AFTMRRFFDEFNAAGMIPVSLIHWQLTGEPMR"
     gene            complement(8521..9462)
                     /locus_tag="PL152_05705"
     CDS             complement(8521..9462)
                     /locus_tag="PL152_05705"
                     /inference="COORDINATES: protein motif:HMM:NF006045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HQR48808.1"
                     /translation="MRRNDVFARLLRDCEPLEAVTCAVAHPCDRDSLLGALEAARRKL
                     IVPVLVGPPARIRAIAEANGADISGCRIVATEHSHASAAAAAAMARAGEVEMLMKGSL
                     HTDELLGFVVAAQSGLRTDRRVSHAFVMDVPAYDRVLLVTDAAINIEPTLAEKADIVR
                     NVIDLAHVLGIAMPKVAILSAVETVNPHIGSTLDAAALCKMADRGQITGGILDGPLAF
                     DNAVSEHAARTKGIRSPVAGRADVLMVPNIEAGNMLAKQLQYFAGADSAGVVLGTRVP
                     IVLTSRADSVRMRIGSAAVAKLMAHARRAASATRPTA"
     gene            9753..9893
                     /locus_tag="PL152_05710"
     CDS             9753..9893
                     /locus_tag="PL152_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48809.1"
                     /translation="MSSGSSSWKGSMSPETSTSASISVCVITRCADAVAPTSISSNVT
                     LR"
BASE COUNT         1503 a         3537 c         3427 g         1527 t
ORIGIN      
        1 cctggcgcga ggaactcgag cgcgactacg gctacacctc gctgcgctgg ctcgaccgcg
       61 acgagacgcg ggcgctgatc gcctccgacc ggtacatcgg cggcctgctc gacagccgca
      121 gcggccacct gcacccgctc aactacaccc tcggcctcgc ccgcgccgcg gaggccgccg
      181 gagcgcgtct gttcgaaggc agccaggtca cgcgccttga ccacgggcca atcgcgaccg
      241 tagcgacgca gcacggtctc gtgcgggcca ggcacgtcgc gctgtgctgc aacgcctata
      301 tcgacgacga ggtctcgagc aggctgcggg cccgcatcat gccggtgggc acctacatcg
      361 tcgcgacgga accgctcggc gaagcgcgca tcagggccct gatgcgcgag aacgtcgcgg
      421 tcaccgacat caactgggtc ctcgactact tccggccatc ggcggaccac cgcctgctgt
      481 tcggcgggcg cgtgagctat tccggactcg acccgctcaa cacggcgcgg gcgacgcgcg
      541 cgcgcatggt caaggtcttt ccccagctcg ccgacgcgag gatcgagtat tcgtggggtg
      601 gctacgtcga catcacgatg agccgggcgc cggacttcgg ccgcctcgcg ccgaacgtgt
      661 actacctgca gggcttctcc gggcacggca tcgcgctgac cgggatggcc ggtcggctcg
      721 tggccgaggc catcgcgggc caggcggagc gtttcgacct gttcacgaag ctcgagcacc
      781 gggacttccc cggcggccgt gcgttcagga ccccggcgct ggtgctcgcg atgctctggt
      841 tccgcctgaa ggacctgctg taagcggcaa tctcaccgat cgctcccgtc gtagcgcgca
      901 ccgctcgtgc aggtctcgtg cacgacgacc gtggagagtt gcgcgagccg aggcttcagt
      961 tcccgccaga tccagcgcgc gagattctcg ctcgtgggat tgtcgagccc aggcacgtcg
     1021 ttgaggtagt gatggtcgag ccgctggtaa agcgggtcga atgcggcgcg gatgtcggcg
     1081 aagtccatca cccagccggt gtgcgcatcc actgccccac tgacgtggat ctcgatacgg
     1141 aaggaatggc cgtgcacccg cgcgcacttg tgcccggccg gcacgttcgg caggcgatgt
     1201 gcgctttcga gggtgaaggt cttgaagatg tccacgcctg cgtccaccgg gtcacgatga
     1261 gggccgcaag gatgcaagcg tggctcgagg aagtcgagcg ccgcgtggac cgaggccgca
     1321 gccccgcgga ctgataagct caccgacatg aaattcggcg aagtgatctc gaccgtccgc
     1381 caggccggcg gcgcatggac ggcgcacatc ccgtcggact gggccaacgg gcgcacggtc
     1441 ttcggcggac tccaggcggc gctgctggtt cgcgggatgc gcggggtgct cggcgatgcg
     1501 cgggcgctgc cactacgcag cctccacgtc accttcgtgg cgcccgtcgc agcgggcaag
     1561 gacctcgtgc tgcgcccgga actgctgcgc atcggcaagt cggccgcgca cgcccgctgc
     1621 gacatgctca cgggcgacgg cctcgtctgc acggccgtgg cgatctatgg cgccgaccgg
     1681 ccctccgccg tgttcatgga aatcccgcgc gtcgaggtga acaccgatcc ggaccagttg
     1741 acgcgctggc cgtacattcc cggtgtcacg ccggaattcg tcaagcatct cgacatccgg
     1801 ctcgcccggg gtgcgctgcc ctactccggg cagctttcgg cgcagtcgac gctcatggcg
     1861 cggctcgtcg actcggactg ctccgcagag gaggccctgg tcgcactctc ggacacgccc
     1921 ccgacaccgg cactctcgat gctcagcaag cccgcgcccg ccgcttcgct caactggatg
     1981 ctggagctgc tcgggcaccc ggacgacctc gagctcgacg cgtggtgcgt catcgacacc
     2041 agcgtgcgcg ctggcagcga cggctacctg agccagacct cgacgctgtg gggaccgtcc
     2101 ggccacgcgt tctcggtcag tcaccagagc gtggcgatct tcgggtagcg gcgcgcagcc
     2161 gcgggccgcc ccacccggtt gccgatcgcc tccgcgggcg tcctgggcgt gtcagtcgga
     2221 ccggcccctg ggcctcagca ggtaagcgac gtggatgcgc gggttcctgc ggaagtcctc
     2281 cgggagcgtc gcgcggctca ggtcttccac gacgaagtcc ccgagggact cgaggtcgag
     2341 gcgaaaccgc tggtagttgc aggaaaacag gatcgcgccg ccgggctcga gcagtcccgc
     2401 gaccgtccgc agcagttcgg cgtggtcgcg ctgcacgtcg aacgattgct gcatgcgctt
     2461 ggatcgcgac gtggtgggcg ggtcgaggaa gacgagcccg tagcgattac gtccggtggc
     2521 agcctgctcg tcgagccagg ccaggcagtc ggcgtgcacg aagccgtgct gaggacccgc
     2581 cagatcgttc aaggcgagat tgcgcttggc ccagtcgagg taggtgttcg aaaggtcgac
     2641 cgtcgtcgag ccggtggcgc ctccgccggc cgcgtacacg gtcgcggtgc ccgtgtaggc
     2701 gaacaggttg agaaaacgcc ggcccgccgc gagctcgccg atccggcgcc gcgtgattcg
     2761 atggtcgagg aacagccccg tgtcgagata gtcggtgaag ttgaccagga accggtagcg
     2821 cccctcgcgc acgacgtgga atcgcgcctc gctgtcggcc ttctcgtatt gcccgccgtg
     2881 ccgctggcgg gtgcgcacct tcagcgtgac gcgatcggcg ggaagtccaa ggacttccgg
     2941 gaggaccgaa agcgcttcgt cgcgacggcc gcgcgcggcg tcccgcgaca cggtcggtgg
     3001 cgcggcgtat tcctgcacgc acgcgtgccg ctcgccgttg ccgtagatgt ccactgcgaa
     3061 ggcgtactcg ggcatgtcgg cgtcgtagac gcgaaagcac gacacgtctt cccgccgcgc
     3121 ccacgactcg agccgcgaca ggttcttcgc caggcggttg gcgaacatct gcgcgccggg
     3181 ccgggctcgc gcggcctcga gccgctcgcg tcggaccgtc tcgacgtccc gctcgacggc
     3241 gtcggccccg agttcgaaac gcagcagccg gcactcgatc gggccgttga agaacgtgtg
     3301 gctgcgatat gcgcgcagtc ccagtgctcg acccagtggc gggttgccgg tgaagacccc
     3361 ggcctcccag ccgggaaatt tctcgacgag cgtgcgaccg agtacaccgt acagcggtcc
     3421 gagcgtgcgc tcgtcttcga ggcgggcgcc gtagggcgga ttcacgagca ccagccctcg
     3481 cgattcggag gcctgcggca gtgaggcaac gtcgcgccgc tcgaaccaga cgtagtcgcc
     3541 caacccggca cgatttgcat tggccagcgc ggcgcgaatg gcgttctggt cgcgatcgaa
     3601 cccccgcaat cgcccctgac ccagagccgc gaggctgcgc cgcgcctccg cagccgcgcg
     3661 cagctcgctc cacaccgacg catcgtgccc gagccagcgc tcgaacccga accgtcgacg
     3721 cagcagccct ggagcagcgt tcgcggcgat cagcgcagcc tcgatgacca gtgtgcccga
     3781 cccgcacatc gggtccgcga aggcaccacc ttccgcggcg atggcgggcc acccgcagcg
     3841 catgagcagt gccgccgcga gattctcctt gagcggcgcc tcgacggctg attgccggta
     3901 tccgcgacgg tgcagcggct cgccactcag gtcgatcccg accgtcgccg ccgaccgcgc
     3961 aagccgcagg ctgacgcgca ggtccggcgc tgcgaggtcc acggatggac gcgtcccgca
     4021 gcgctcccgg aaacggtcgg cgatcgcgtc cttggctttc tgcgccacga actgcgtgtt
     4081 cctgatccac gagggccctt caccgaccac ctccaccgcg agcgttccgt cgggtccgag
     4141 gtgatcctcc cagtgcattg ccttgagcgc gccgtacatc gcgtcggcgg aatcgagcgg
     4201 caccgtaccg agggtgagca gtacccggct cgcggtccgc gactcgaggc aggcgcggta
     4261 tccatccgcc agggtcccgt cgaagcccac gccggcgggg tgctcgcgcg tcacgcggat
     4321 cccgagcgcg cgcaactcgg cggcgaggat gtccggcacg tgcctcgggc aggcggcgaa
     4381 gaattcgtgc gtgcctgccg gcggcggatc actcactggc atgcgtcccg tggtcgagca
     4441 cgacgaggtc ccgcggcgcg acacggccca ggcaacggcg caggatccgg tacgagtcgg
     4501 cgtcgaatcc ggatccatcc cgcgtgcgca gcagaccggc gatctcgcct tcgtcgccag
     4561 ccgtaccgag atgctgccag cgatcgatga cgtgcaggat gctgccctcg ccgcccggcg
     4621 cgcgctcgcg gatgccgaca gccccgcgga acggccatgg ctggatccgc agcgacgcga
     4681 gcgcaagccg gagtcgcgca tcgtgcatcg gcagcggctc gcggcccgca cacgcgccac
     4741 ggcaccgacc gagctggctc gcgaagcacg acccctcccc ctgctccagt cccagcacct
     4801 tgaagcacag ctgggcctcg cgcgccttgc cctcgagcgc gcgccacgcg tcacgctcgt
     4861 cgcggaacgg gccatacgcc tcggcgtcgc ccgccacgtc gcccggctcc acgacttcga
     4921 tgcgcgggcg cagcgccccg gcgacctcgt ccagccgtat gcagtaggcg gcggatgccc
     4981 gacgcaacct gcggttcgcg gccggcgcga gttccttgac cagccgtgac tcgagcagca
     5041 gcgcgcccag ttcgcctccc gtctcgaccc actcgacctc gcgtacctgc cgcgcgaggt
     5101 tcgactcctt gctcgcgcgc tggccggtcg cgaagtggtc cagcacgcgg ctgcgcaggc
     5161 tgcgactcat gccgacgtac agcggtgtgc cgccctcgcc gcgaaagatg tagacacccg
     5221 cgccttccgg caggtcgtcc gcgaggtccg ccggcaggtg cgccggcagg cgggcgtccc
     5281 gcctcgcggc ctcgatggcc tcctcgaccc ggccggcgcc gacggattgt tcgaacgcag
     5341 cgagcaacgc gggcagcacc gcggcatccc cgagcgcgcg atgccgctgc ccgcattcga
     5401 gcccgaatcg ctcgatcagc gtgtcgaggt tgtgccgcgc gtgctgcgtg aacagcgcac
     5461 gcgacagccg caccgtgcaa agcaccggcg ccgtgaactt ctcgtgctcg cgccggaact
     5521 cgttgcgcag gaacccgtag tcgaagcggg cattgtgcgc gacgaacagg cggccggtga
     5581 gtcgcgaccg gatgtccggc aacaggtccc ggaaggtcgg cgcgtcctcg accatcgcat
     5641 tcgagatgcc cgtgaactgc tcgattgagc ggggaattcg ctgcccaggg ttcacaaggc
     5701 tgctccactc ctcgaccacg gcgccgtcgt cgagcagcac cacgcccacc tcgatgatcc
     5761 ggtgctgcgc agccactcct cccgtcgtct cgaggtccac gcacgccacc cggccgcgca
     5821 gtgcgggggc gccaagtccg atcgagggcg tgagggccat tctggccacc gagcttcaga
     5881 gcgagccgtc accgtccagc gtcatgatcg gcccgtacag gtccggccgg cggtcgcgga
     5941 acacgcccca ggcgtgccgc tgggcgcgga tcgcgtcgag gtcgaaggtg gcggtgagca
     6001 cggcttcgcc ggaccggtcc gcctcgacga ccttctcgcc ggtcggtccc gcgatgaacg
     6061 acgacccgta gaacgtcatc tcgtacttct cgccgcgctc gacaccgatt cggttcgacg
     6121 cgaccagcgg catcacgttc gcggcggcgt gaccctgcat gcagcgttgc cagtgatcgc
     6181 gcgaatcgat gctcgcatcc tggggttccg agccaatagc ggtcggatac aggagcacct
     6241 ccgctcccat gagcgccatg cagcgcgcgg cttccgggaa ccactggtcc cagcagatgc
     6301 cgacgccgag gattccgtag cgcgtgtgcc agacgcgaaa gccggtgtcc ccgggcgaga
     6361 agtagaactt ctcgtgataa ccggggccct cgggtatgtg cgacttgcgg tacacgccga
     6421 gcacgctgcc gtcggcatcg atcatggcga gcgagttgaa gtaggcattg ttcgcccgct
     6481 cgaacacgct cagcggcagc acgaccgcga gctcgcgcgc cagtgcccgg aagcgttcga
     6541 cgaccgggct gccggcgatc ggccgtgcca gcgcgaaatg gctggcgagg tggtccttgc
     6601 agaagtacgg ggtctcgaac agttcctgca gcaggatgac ctgcgcgccc tggcgcgcgg
     6661 ccgcacgcac cgcgcgctcg gccgagtcga gattgcgggc cgcctcggtc gagcaggcga
     6721 actgtgtggc agcgagcgtg actttcatgg cggatccagc ggccggggcg ggtctcgaat
     6781 tgtagcgaaa gcaggcaccg gttgacggcc gcgcggcagg ccctcagcgc atcggctcac
     6841 cggtgagctg ccagtggatc agcgacaccg ggatcatgcc cgcggcattg aactcgtcga
     6901 agaatcgccg catcgtgaag gcctcgccga gctgccggcg gcggtccttg atcgtctcct
     6961 cgatcaggta cttgccggtg acgtagctcg tgccgtagcc gggctggcgc aggtagagct
     7021 gctgctcgaa gccgagcagg tccaggtcgg gccgcatcca gccgcgcggc gtccactcga
     7081 cctggaaggc cttcgcctgc cgcaggtcga actcgttggc ctgcgcgtac agcgacgcga
     7141 gtccgcgcgc acagcgttgc gcgagcatga tccacacgat ctcccgcgcg cgcgggttgt
     7201 cgtcgtagta gcccgcgtgc aggatgatct cctccatcgc ggtcgccatg ccctcggcgc
     7261 gcgtgtccca ggcgttgtaa gcccaggctt cgcgccggat cgggctggga ttgggcttgc
     7321 gaaccagcca ctcgtggtcg aaccagtggt agaagtgcgc gtagagcgtg acgggctcgt
     7381 agtggctcgc gatcgagaag aaattgcgct gctcgaccgg cacgtacggg tcgatgcgcc
     7441 ggcgcagcgc gggatcgagg taatcgtcca cctcgatcag gtcccgctgc cggaggaacg
     7501 ccaggtactt cgtaatggac gcgttcgcgc gcccggcgtg ctcctcggcg ctggcggccg
     7561 gcgtaagctg cgggaggccg cgattgcgct gctcctcgag cgccagcgac gaccacgcgc
     7621 gcgccagttc gctcttgagc agcatgactt ggtcgttcca ggtgagcggc acgaggtgca
     7681 cattgcggag actccagtcg taggcctcct tgccgacacc ggagggtccg gtcttcgacg
     7741 gcgcctgcgc atcgagccac gcaatgaact ccgccgtggc cgtggccgca gcggcaatcg
     7801 catcgcgcag gtccggtccg tagtcccggg tcttcgacgc aaggtcagcc agggcctcgt
     7861 cctgctgccg catcgtgccg gtccccgtga tccacaggtc gtgcgcatcg cccgtcaggt
     7921 tgcgccgcgc ctgcgacagc agtggcggga ccgtgcgcag ttcgcgggtg agccgggcct
     7981 cgtcctggcg cgacagcgga aacgcgtact gccagagttc caccggtgcg tggttgaccg
     8041 ggccctcgtg ctccggcgtg tcgctctgct cggtccacac cgaacgatag aaagcggggt
     8101 cgcgcgccca cggtcgcagc cagcggatgt cgaactcgag gccgttcatc tgcgcgcgca
     8161 cgaggtcgta gtcgaccttc cgggccaccg gccagtccgc aggatcgatc gcggcgagcc
     8221 gcgcctggta acgcgcgagt tcctcgctct tcctcgcgat ggccgccacc gtgtagtccg
     8281 gggcgccgtc gagcggcggc ggcggctcga atcgccgcca ctcctcgtac agcttcagga
     8341 ggtcgtcgta cgaattgccg gaggccatcg cagacccgct cacggcgagc atcgccaggg
     8401 ggaatacccg ggcggccacc gccaccatcc gcaaccaatg ccgcatcgcg ccgctccctt
     8461 cattgcatcc ccgtcggtgg ccgatcgccg ccgcaccccg tccgagaagt cagcaaccga
     8521 tcatgccgtc ggccgcgtcg ccgaggccgc gcggcgcgca tgcgccatga gcttcgcgac
     8581 cgcggcggag ccgatgcgca tgcgcacgct gtcggcgcgg ctggtgagca cgatcggcac
     8641 acgggtcccg agcacaactc cggcactgtc ggcgcccgcg aaatactgca gttgcttggc
     8701 gagcatgttg cccgcctcga tgttgggcac catgagcacg tccgcgcggc ctgccaccgg
     8761 cgagcggatg cccttggtgc gtgccgcgtg ctcgctcacg gcgttgtcga atgccagcgg
     8821 cccgtcgagg atcccgccgg tgatctggcc gcggtccgcc atcttgcaca gggccgcggc
     8881 gtcgagcgtc gaaccgatgt gcggattcac cgtctcgacg gccgacagga tcgccacctt
     8941 gggcatcgcg atgccgagga cgtgcgcgag gtcgatgacg ttgcgcacga tgtccgcttt
     9001 ttccgcaagc gtcggctcga tgttgatggc ggcgtccgtc acgagcagca cgcggtcgta
     9061 ggcgggcacg tccatcacga acgcgtggct cacgcggcgg tcggtacgca gtcccgactg
     9121 cgcagcgacc acgaagccga ggagctcgtc ggtgtgcagg ctccccttca tgagcatctc
     9181 gacctcgccc gcgcgcgcca tcgccgcggc cgcggccgcc gaggcgtggc tgtgctccgt
     9241 cgcgacgatc cggcagccgg agatgtcggc gccgttcgcc tcggcgatgg cccggatccg
     9301 cgccggcgga ccgaccagca ccggcacgat cagcttgcgg cgggccgctt cgagcgcccc
     9361 gagcagcgaa tcgcggtcgc aggggtgcgc gaccgcgcag gtcaccgcct cgagcggctc
     9421 gcagtccctg agcaggcggg cgaagacgtc gttgcgccgc aggatgattt ccggggtcgc
     9481 gatgtcggag tactgcagcc tgcggcgcgg ggccccacag cacccgccgg ccgccgacgt
     9541 gcgcctcgca gtcgaacgcc acgaggcccg acccggcgtg cttctcgcgc accgtgaccg
     9601 atgccgtgag ttcgtcgccc acctgcacgt ggccggcgta ctccagttgc tgcgagacga
     9661 tgcgcgttcc cgggcccggc agcttgcgct ccagcagccc ggagatcacc gcgccgacgc
     9721 cgaccgcgtc gacgcacagg cgtccggcca gcgtgtcgtc cggatcttcg agctggaagg
     9781 gctcgatgtc gcccgagacc agcaccagcg cctcgatctc ggtctgcgtc atcacgcgct
     9841 gcgcggatgc cgttgcgccg acctcgatct cgtcgaacgt gacgttgcga taggcgctca
     9901 cgagagttcc cgcaggttca ggagagatcg atccaggtcg tcttgagctc ggtgtacttg
     9961 tcgaacgcgt gcagcgactt gtcgcggccg gtgc
//
LOCUS       DAOMFD010000069         9859 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_1981_length_9859_cov_35.486332, whole genome shotgun sequence.
ACCESSION   DAOMFD010000069 DAOMFD010000000
VERSION     DAOMFD010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9859)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9859
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1981_length_9859_cov_35.486332"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PL152_05715"
     CDS             complement(<1..267)
                     /locus_tag="PL152_05715"
                     /inference="COORDINATES: protein motif:HMM:NF025012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48810.1"
                     /translation="MITTRACIVATVIAVHAAGCGYKGPLTLPEKGGPVTIRPGTQAP
                     AATTPAGSPPGTEASGAAAGEAPAAAQTAPAQASPANPGTAPAQP"
     gene            complement(264..716)
                     /locus_tag="PL152_05720"
     CDS             complement(264..716)
                     /locus_tag="PL152_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog fold thioesterase"
                     /protein_id="HQR48811.1"
                     /translation="MSIWFQPFTLDEIQQYSRGSMVEHVDLRFTEIGPDFLRATLPVD
                     ARTTQPFGLLHGGASVMLAETLGSVAATHCVDMAKYFCVGQEINANHIRPARSGRVTG
                     TARPIHIGTRSQVWDIRIEDDRGRLVCISRLTMAVLEGSSLAKAGGAQ"
     gene            850..2631
                     /locus_tag="PL152_05725"
     CDS             850..2631
                     /locus_tag="PL152_05725"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HQR48812.1"
                     /translation="MSDYHAPLKQMRFTMEHLADFGEVRALPDFEAVDADTAEAVLEE
                     AAKFAGAVLAPINVLGDREGVKVVGDDVQVPAEFTDAYGKFCEGGWPGIALNPEFGGQ
                     GLPKALSVACDEMWAASNVSFALCPELSQGAILAMDRHGTDALKATFLEKMVSGQWAG
                     TMCLTEPQAGSDLSSLTTRAEPKGDVYLITGQKIFITWGDHPMTENIVHLVLARLPDA
                     PPGTKGISLFAVPKFKVSADGSLGARNDNRPVSVEHKMGIHASPTCVLSFGDHGGAEG
                     YLIGKPNEGLAAMFVMMNYMRLGVGLEGVGLADRAYQHAVAYARERVQGRAPGEKGRV
                     AIIRHPDVRRMLLAMRALTQASRAICYYTAACLDRAAHAADAAAAETWQARADLLTPI
                     AKAWSTEIALEVTSHGIQVHGGMGFVEETGAAQFYRDARIAPIYEGTNGIQAIDLVGR
                     KLLRDGGASVSAFVSDMRAVDAPLANAGEDLAIVRSALGRALDELVQCSTHILTGEKK
                     DPELPGAVAFHYLMLLGTVIGGWQLARGALAAMQQLASGGDKAFLEAQVVMARFYAEQ
                     VLPRCTAHAASIRAGSGTIMALAADHF"
     gene            2639..3232
                     /locus_tag="PL152_05730"
     CDS             2639..3232
                     /locus_tag="PL152_05730"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: protein motif:HMM:NF016248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008813 - chorismate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HQR48813.1"
                     /translation="MSAHAAGAASAPEGAGDRWVRLDDCLADAPPVLHAWLAEPGLLT
                     ARVRRTCGDATEFRLLRLERAPLAAGLAHRMGVTDVNCLVREIEFACGSIRWIFAQSV
                     FPDSTLRLHPWLAELGSAALGESLLGRHDVSREPLEYRELSPADALAAAAGGIDGHAW
                     ARRAVYRLSGAPILVQEVFLPALADDGRDPHRPGSTA"
     gene            3229..3915
                     /locus_tag="PL152_05735"
     CDS             3229..3915
                     /locus_tag="PL152_05735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQR48814.1"
                     /translation="MSYEFAPPIAPTVEIAGSQTRFPVHRIYCVGRNYAAHAREMGMD
                     PEREPPFFFSKPADAVVANGATVPYPPRTSNLHHEIELVVAIGEGGRDIPVANALAHV
                     FGYAVGNDLTRRDLQFAAREKGQPWDTSKGFDQSAPVSAIRRVADIGHPSRGAIWLEV
                     NGETRQKADLCEMIWNVPEIVSELSALFELKPGDLVFTGTPAGVGPVVRGDSLVGGIE
                     GLDTLRNTIA"
     gene            3935..4585
                     /gene="maiA"
                     /locus_tag="PL152_05740"
     CDS             3935..4585
                     /gene="maiA"
                     /locus_tag="PL152_05740"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016034 - maleylacetoacetate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006572 - tyrosine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HQR48815.1"
                     /translation="MALTLYTYWRSSAAFRVRIALNLKGLEYHSVPKHLLRGGGEQRQ
                     SDYLALNPQGLVPALDDDGTLVTQSLAICEYLEEKWPEPRLLPGDAASRARIRAMALA
                     VACDIHPLNNLRVLQYLRGEFAADEATVNRWARNWIGRGFEALDQLITRNSADGRHCV
                     GGGVTLADVFLLPQVFNARRVELDLAPFPRIAAVAAHLESLPAFAAARPDAQPDAE"
     gene            complement(4596..6449)
                     /locus_tag="PL152_05745"
     CDS             complement(4596..6449)
                     /locus_tag="PL152_05745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48816.1"
                     /translation="MSLIAELRRRNVFRVAMLYAVAGWVLLQVGDLLFGALGVPPWGI
                     KLLLGLLLLGFPMAVVFAWVYELTPEGLKREHEVDRNASITQETASKLNILVAVLLVV
                     AIGLVVADRFIPHGKGAATSLQATSDSAAASGPAATSPPAAGGASSSSLASIAVLPFV
                     NMSDDKANEYFSDGLTEELLNVLANVPGLRVIARTSSFAYKGKEVKIADVAHDLNVDH
                     VLEGSVRKSGNRVRITTQLIRSSDSSHLWSQTYDRDLNDIFAVQDEISSEVVDALKVK
                     LLKPATASSAEAGGTHDPAAYEAYLRARHLRLDGGEGETTLRGAIAAYDEAIRIDPQY
                     ARAYAGKAEAQNALASNGYVPFDSGFKAARATAERALELAPDLPDALIPLSYIQFNVD
                     MDPAAADASVERAMRLSPGSIDVQLTYSAFAASMGWRDRSIPSARNAVELDPINPRAY
                     VNLAGVLGWAGQLEEAERVARHLVSLAPGRPSGHYTLGSVLLLQGRADEALAEFDQES
                     ITWQRMFGRAITFARLAKPDLARQEIAAMQKVLGDAASYQYAQVYTALGDRDQAFQWL
                     AMARKVHDPGLMGQMYADPFLDPIRSDPRYTALARELGFLTARPDTSAAAR"
     gene            complement(6564..7751)
                     /gene="argH"
                     /locus_tag="PL152_05750"
     CDS             complement(6564..7751)
                     /gene="argH"
                     /locus_tag="PL152_05750"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HQR48817.1"
                     /translation="MTRLWDKGAPLDERVLRYTAGEDHALDDRLVEYDVRASIAHAEA
                     LHECGLLDAASLAAIRAGLTEIGAAHARGEWSVTLELEDGQTALETLLTQRVGEAGAR
                     LHAGRSRNDQVLAALRLYLRDAVESLAGGARDVAAALEALAADQGAVVLPGYTHMQQA
                     MPSSVALWARGFAVEIQDDADALRHAHRRIQKNPLGSAAGYGTPNLPLSRDATRTRLG
                     FPVTQEPVTAVQLSRGKAEAEVLFQVALLMQDLGRLASDLLLFYTREFGFVELPDAFT
                     TGSSIMPQKRNPDVFELVRGRTATAQACLTEVLAITAKLPSGYQRDLQLIKAPLFRSI
                     DLCRESLAIMAAAIPQVRFAADRIRLDPDIHAAAEANRLVASEGIPFREAYRRVGRKL
                     HET"
     gene            complement(7794..8723)
                     /gene="argB"
                     /locus_tag="PL152_05755"
     CDS             complement(7794..8723)
                     /gene="argB"
                     /locus_tag="PL152_05755"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF012899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HQR48818.1"
                     /translation="MIGHKGEQAVTVRALRGAAPYIHMYKGKVFVIKTGGGAFRDGAA
                     MRGFMEQVAILHHLGIRVVLVHGGGPQLDTVADKFGVETRMVQGRRVTDGGAIDVACM
                     VLNGLINTRLLGLCREFGIDAIGLSGVDAGLVKAHKRPPVTLASGETVDYGMVGDIDD
                     VDGSVITRLLDAGFLPVVSPLSCDSLGHLLNINADTVAAAIGGAMDAEKLILCTGAPG
                     ILERLDDPHSLISYTDVAGLRRLREEGAISDGMLPKSSAIEAAIRGGVRRVHVISYAA
                     PDALLAEIFTNEGTGTLVVADTKALSAAEQGAG"
     gene            complement(8729..9727)
                     /locus_tag="PL152_05760"
     CDS             complement(8729..9727)
                     /locus_tag="PL152_05760"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: protein motif:HMM:NF003384.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HQR48819.1"
                     /translation="MQRFLDLADFTRDQVQDLVALARRLEERPEPDALSGKILGLLFM
                     NPSLRTLASFQAGMMRLGGRSFVVTPGQGTWQFETRVGAVMNAGAAEHVREAIPVLSS
                     YCDALGIRSFAEGKDLAADLAETQFLSMAGLCDKPFVNMESATNHPCQALADWKTMDD
                     LAVPRTAKFVLSWVNHPRALPLAVPAATVHMAAQRGMEVVVLRPDGYSLPESLMAKAR
                     AAAARSGGQVTETSDRESALAGAHVLYAKEWGSAQCYGDAEAEAALRAPLSAWQVRED
                     WFRSARADCHFMHCLPVRRNVAVADEVLDGPRSRVVQEAANRMTVQMAVLYSLLKG"
BASE COUNT         1461 a         3404 c         3500 g         1494 t
ORIGIN      
        1 gggctgcgca ggggctgtgc ccgggttcgc gggcgatgcc tgggctggcg cggtctgtgc
       61 cgctgccggg gcttcgccgg ccgctgcacc gctcgcctcc gtgccgggcg gcgaccccgc
      121 tggagtcgtg gcggcaggcg cctgcgtgcc ggggcggatc gtcaccggcc cgcctttctc
      181 cggcagcgtg agcggtccct tgtagccgca gccagcggcg tgcaccgcta tcaccgtcgc
      241 aacgatgcag gcgcgggtcg tgatcactgc gcacctcccg ccttcgccag gctcgagccc
      301 tccagcaccg ccatcgtcag gcgggaaatg cacaccagcc gaccgcgatc gtcctcgatg
      361 cggatgtccc agacctggga gcgcgtgccg atgtggatcg ggcgggcggt gcccgtgact
      421 cggccgctcc gcgccgggcg gatgtggttc gcgttgattt cctgcccgac gcagaagtac
      481 ttcgccatgt ccacgcagtg cgtcgccgcg acgctgccga gcgtctcggc cagcatgacg
      541 gaggcgccgc cgtgcagcag cccgaacggc tgggtcgtgc gcgcgtccac gggcagcgtc
      601 gcgcgcagga agtccggccc gatctcggtg aagcgcaggt cgacgtgctc caccatgctg
      661 ccgcgcgagt actgctggat ctcatccagc gtgaacggtt gaaaccagat gctcattcgg
      721 gggttcctgg ggccggggtc gggattgtaa ccgccggccg gcccgcggcc gccggcctgt
      781 tttccgtggc gccgggggcc ccgaaagggc agaattgctg caaagcaaaa gccgtgccgg
      841 agcaccccga tgagcgacta ccacgccccg ttgaagcaga tgcgattcac catggagcac
      901 ctcgcggact tcggcgaggt tcgcgcgctg ccggacttcg aggccgtgga tgccgacacg
      961 gccgaggcgg tgctcgagga agccgcgaag ttcgccggcg cagtgctcgc accgatcaac
     1021 gtgctcggcg atcgcgaagg cgtgaaggtc gtcggcgacg acgtgcaggt gccggcggaa
     1081 ttcaccgatg cctacggcaa gttctgcgag ggcgggtggc ccggcatcgc gctcaacccg
     1141 gagttcggcg ggcaggggct gccgaaggcg ctgtccgtcg cgtgcgacga gatgtgggcc
     1201 gcgtcgaacg tctcgttcgc gctgtgcccg gaactgtcgc agggcgcgat cctcgcgatg
     1261 gaccgccacg gcacggacgc gctcaaggcg acgttcctcg agaagatggt gtcgggccag
     1321 tgggcgggca ccatgtgcct caccgagccg caggccggct ccgacctgtc gagcctgacg
     1381 acgcgcgccg agccgaaggg cgacgtctac ctcatcacgg gccagaagat cttcatcacc
     1441 tggggcgacc accccatgac ggagaacatc gtgcacctcg tgctcgcgcg cctcccggac
     1501 gcgccgccgg ggaccaaggg catctcgctg ttcgcggtcc cgaagttcaa ggtgagcgcc
     1561 gacggctcgc tcggcgcgcg caacgacaac cggccggtct ccgtggagca caagatgggc
     1621 atccacgcga gcccgacctg cgtgctgtcg ttcggcgacc acggcggcgc ggagggctac
     1681 ctgatcggca agccgaacga ggggctcgcc gcgatgttcg tgatgatgaa ctacatgcgc
     1741 ctcggcgtcg gactcgaggg cgtgggcctc gcggatcgcg cgtaccagca cgccgtcgcc
     1801 tacgcgcgtg aacgcgtgca gggccgcgcg ccgggcgaga agggccgggt cgcgatcatc
     1861 cgccatccgg acgtgcgccg catgctgctc gcgatgcggg cgctgacaca ggcgagccgg
     1921 gcgatctgct actacacggc ggcgtgcctc gaccgcgccg cccacgccgc ggacgccgct
     1981 gccgccgaga cctggcaggc gcgcgcggac ctgctcacgc cgatcgcaaa ggcgtggtcc
     2041 acggagatcg cgctcgaggt gacgagccac ggcatccagg tgcacggcgg catgggattc
     2101 gtcgaggaga cgggcgccgc gcagttctat cgcgacgcgc gcatcgcgcc gatctacgag
     2161 ggcaccaacg ggatccaggc catcgacctc gtgggccgga aactgctgcg cgacggcggg
     2221 gccagcgttt ccgcgttcgt gtcggacatg cgcgcggtcg atgcgccgct cgcgaatgcg
     2281 ggcgaggacc tcgcaatcgt gcgcagcgcg ctcggccggg cgctcgacga gctcgtgcag
     2341 tgctcgaccc acatcctcac cggcgagaag aaggacccgg agctgccggg ggccgtcgca
     2401 ttccactacc tcatgctgct cggcacggtg atcggcggct ggcagctcgc gcgcggggcg
     2461 ctggcggcga tgcagcagct cgcgtccggc ggcgacaagg cgtttctcga agcccaggtc
     2521 gtgatggcga ggttctacgc cgagcaggtc ctgccgcgct gcaccgcgca tgccgcgagc
     2581 atccgcgccg gcagtgggac gatcatggcg ctggcggccg accacttctg accgtgcgat
     2641 gagcgctcac gcggccggtg ctgcgagcgc gcccgaaggc gccggtgacc ggtgggtgcg
     2701 cctggacgat tgccttgccg acgcgccgcc ggtcctgcac gcgtggctcg ccgagccggg
     2761 actgctcacg gcgcgcgtgc gccgcacctg cggcgatgcg accgaattcc gcctgctgcg
     2821 gctcgagcgt gcgccccttg cggcgggact ggcccatcgg atgggggtga ccgacgtgaa
     2881 ctgcctggtc cgtgagatcg agttcgcgtg cggcagcatt cgctggatct tcgcgcagtc
     2941 ggtgttcccg gactcgacct tgcgcctgca cccgtggctc gcggaactcg gcagcgccgc
     3001 gctcggcgag tcgctgctgg gccggcacga cgtgagccgc gagccgctcg aatatcggga
     3061 gttgtcaccc gccgatgcac tcgcagccgc cgctggcggc atcgacggcc acgcgtgggc
     3121 gcgccgtgcc gtctatcggc tgtcgggcgc gccgatcctg gtgcaggaag tattcctgcc
     3181 ggcactggct gatgacggcc gggatccgca ccggcccggg agcactgcat gagctacgaa
     3241 ttcgcgccgc cgatcgcacc cacggtcgag atcgccggca gccagacgcg ctttcccgtc
     3301 catcgcatct actgcgtggg gcgcaactac gccgcgcacg cgcgggagat gggcatggac
     3361 ccggagcgcg agccgccgtt cttcttctcg aagccggccg atgccgtcgt cgccaatggt
     3421 gcgacggttc cctatccgcc gcgtacctcg aacctgcacc acgagatcga gctcgtggtg
     3481 gcgatcgggg agggcggccg cgacatcccg gtggcgaacg cgcttgcgca cgtgttcggc
     3541 tatgcggtgg gcaacgacct cacgcgtcgc gacctgcagt tcgccgcgcg cgagaagggc
     3601 cagccctggg acacgtcgaa gggcttcgac cagtcggcgc cagtgagcgc gatccggcgc
     3661 gtcgccgaca tcggtcaccc gtcgcgtggc gcgatctggc tcgaggtgaa cggcgagacg
     3721 cgccagaagg ccgatctctg cgagatgatc tggaacgtgc cggagatcgt gagcgagctg
     3781 tcggcgctgt tcgagctgaa gccgggcgac ctcgtcttca ccggcacgcc ggccggcgtc
     3841 ggtcccgtcg tgcgcggcga cagcctcgtc ggcggcatcg aggggctcga cacgctgcgc
     3901 aacaccatcg cctgagcggc ggggagtacc ggccttggcg ctcacgctct acacctactg
     3961 gcgcagctcc gcggcattcc gcgtgcgcat cgccctcaat ctcaaggggc tcgaatacca
     4021 ctccgtcccg aagcacctgc tgcgcggcgg cggcgagcag cgccaatccg actacctcgc
     4081 cctgaatccg caggggctgg tgcccgcgct cgacgacgac ggcaccctcg tgacgcagtc
     4141 gctcgcgatc tgcgagtacc tcgaggagaa gtggccggag ccgcggctgc tgccgggcga
     4201 tgcggcctcg agggcgcgga tccgtgcgat ggcgctggcg gtcgcctgcg acatccaccc
     4261 gctcaacaac ctgcgcgtgc tgcagtacct gcgcggggaa ttcgccgcgg acgaggcgac
     4321 ggtcaaccgg tgggcgcgga actggatcgg ccgtggcttc gaggcgttgg accagctgat
     4381 cacgcgaaat tccgcggacg gccggcactg cgtaggcggc ggcgtcacgc tcgccgatgt
     4441 gttcctgctg ccgcaggtct tcaatgcgcg tcgcgtagag ctcgatctcg cgcccttccc
     4501 gcgcatcgcc gcggtcgcgg cgcatctcga gtcgttgccg gcattcgcgg cggcgcgtcc
     4561 cgacgcccag ccggacgcgg agtagggccg gcgattcagc gcgcggcggc cgacgtatcg
     4621 ggccgtgccg tcaggaagcc cagctcccgc gccaaggccg tgtaacgcgg atcggaacgg
     4681 atcgggtcga ggaacggatc cgcgtacatc tggcccatga gtcccgggtc gtgcaccttg
     4741 cgggccatcg cgagccactg gaatgcctgg tcccggtcgc cgagcgccgt gtagacctgc
     4801 gcgtactggt acgaggccgc atcgccgagc actttctgca tcgctgcgat ttcctggcgc
     4861 gccaggtccg gcttggcgag gcgcgcaaaa gtgatggccc gcccgaacat gcgctgccag
     4921 gtgatcgact cctggtcgaa ttccgcaagc gcctcgtcgg cgcgcccctg gagcagcagc
     4981 acggagccca gcgtgtagtg accggacgga cgccccgggg cgagcgagac aagatggcgc
     5041 gccacgcgct cggcttcctc gagctggccg gcccacccca gcacgcctgc gaggttcacg
     5101 tatgcgcggg gattgatggg gtccagctcc accgcattgc gtgcggaggg gatcgaacga
     5161 tcacgccacc ccatgctggc agcgaaggcc gaatacgtga gctgcacgtc gatgctgccc
     5221 gggctcaggc gcatggcccg ttccaccgac gcatcggccg cggccggatc catgtcgacg
     5281 ttgaactgta tgtatgagag gggtatcagg gcgtcaggca gatccggcgc cagctcgagg
     5341 gcgcgctcgg cggtcgcccg tgccgccttg aagcccgagt cgaatgggac gtagccattg
     5401 gacgccagcg cattctgcgc ctcggccttg cctgcgtagg ctcgcgcgta ctgcgggtcg
     5461 atgcggatcg cctcgtcata cgccgcaatt gcgccgcgca acgtcgtctc cccctcgcca
     5521 ccgtcgagcc tcaggtgacg ggcccgcagg taggcttcgt acgccgcggg atcgtgcgtg
     5581 ccccccgcct cggctgagga cgctgtcgcc ggcttcagca gcttgacctt gagcgcgtcg
     5641 acgacctcgc tcgagatctc gtcctgaacg gcgaagatgt cgttcaggtc gcggtcgtag
     5701 gtctgcgacc agaggtgcga gctgtcggac gagcggatga gctgggtcgt gatgcgcacg
     5761 cgattgcccg acttgcgcac gctgccttcg agcacgtggt ccacgttcag gtcgtgcgcg
     5821 acgtccgcga tcttgacctc cttgcccttg tacgcgaacg acgaggtgcg cgcgatcacg
     5881 cgcaggcccg gcacgttggc gagcacgttg agcagttcct cggtcaggcc gtcggagaaa
     5941 tactcgttgg ccttgtcgtc gctcatgttc acgaagggca ggacggcgat cgaggccagg
     6001 ctcgaactgc tcgcaccgcc cgccgcagga ggggaggtgg ccgccggacc ggacgcggcc
     6061 gcgctgtccg aagtcgcctg cagggaagtc gccgcgccct tcccgtgggg gatgaaacgg
     6121 tctgccacga cgagtccgat ggccacgacc aggagcacgg cgaccagtat gttcagcttg
     6181 gaggccgtct cctgcgtgat ggacgcattg cgatcgacct cgtgctcgcg cttcaggccc
     6241 tcgggcgtga gttcgtagac ccacgcgaag accacggcca tcgggaagcc gagcagcagc
     6301 agcccgagca ggagcttgat gccccacggc ggcacgccga gcgcgccgaa cagcaggtcg
     6361 ccgacctgca gcaggaccca gcccgccacg gcgtagagca tcgcgacgcg aaacacgttg
     6421 cgacgcctca gctccgcgat gagcgacatg aattcccctg attgcgcgcg gccgaacgca
     6481 aggatgacgg cgccgccgcg aatgtcaacg ccgggtgctg ccgagtgcgg cccggtgccg
     6541 ccaggcagtg cgccggtgcg gtgtcaggtc tcgtgcagct tcctgccgac cctgcggtag
     6601 gcctcgcgga acgggatgcc ttcgctcgcg accagccggt tcgcctcggc ggcggcgtgg
     6661 atgtcggggt cgaggcgaat ccggtcagcg gcaaaacgaa cctgcggaat cgcggccgcc
     6721 atgatggcca gcgactcgcg gcagaggtcg atcgagcgga acagcggcgc cttgatgagc
     6781 tgcaggtcgc gctggtagcc ggacggcagc ttggccgtga tggcgagcac ctcggtcagg
     6841 caggcctgcg ccgtggccgt tcgaccgcgc accagctcga acacgtcggg gttgcgcttc
     6901 tgcggcatga tcgacgagcc ggtcgtgaac gcgtcgggca gttccacgaa gccgaattcg
     6961 cgcgtgtaga agagcagcag gtcggaggcg agccggccga ggtcctgcat cagcagcgcg
     7021 acctggaaca gcacctcggc ctcggccttg ccacgcgaca gctggaccgc cgtcacgggt
     7081 tcctgggtga cggggaatcc gagcctggtg cgcgtcgcgt cgcggctgag cggaaggttg
     7141 ggggtgccgt agccggcggc tgaacccagc gggttcttct ggatccggcg atgcgcatgc
     7201 cgcagcgcat cggcatcgtc ctggatttcg acggcgaatc cgcgggccca caatgcgacc
     7261 gagctcggca tggcctgctg catgtgcgtg tagccaggga gtacgaccgc accctggtcc
     7321 gcagcgagtg cctcgagcgc ggcggccacg tcgcgcgcgc ctcccgcgag cgactccact
     7381 gcatcgcgca ggtagagccg cagtgcggcg agcacctggt cgttgcgcga acggcccgca
     7441 tgcaggcgcg cgcccgcctc gccgacccgc tgcgtgagca gggtctcgag cgcggtctgg
     7501 ccgtcctcga gttccagggt caccgaccac tcgccacgcg catgcgcggc accgatctcg
     7561 gtgaggcccg cgcggatggc ggcgagcgac gcggcgtcga gcaggccgca ctcgtgcagc
     7621 gcttccgcgt gcgcgatgga cgcccggacg tcgtactcga cgagccggtc gtcgagcgcg
     7681 tggtcctcgc ctgccgtgta acgcagcacc cgctcgtcga gcggtgcgcc cttgtcccac
     7741 agtcgtgtca tctcccgtgc tcccggacgc gcccgacgcg ccctgtcatt gcctcagccc
     7801 gcgccctgtt cggccgcgct cagcgccttg gtgtccgcca ccaccagcgt gccggtgccc
     7861 tcgttggtga agatctccgc gagcagggcg tcgggagccg cgtacgagat cacgtgcacg
     7921 cggcgcacgc cgccgcggat cgccgcctcg atcgcgctcg acttcggcag catcccgtcg
     7981 gaaatcgcgc cttcctcgcg cagcctgcgc aggcccgcca cgtcggtgta ggaaatgagc
     8041 gagtgcggat cgtccagtcg ctcgaggatg cccggcgcgc cggtgcagag gatgagcttt
     8101 tccgcgtcca tggcaccgcc gatcgcggct gcgacggtgt cggcattgat gttgagcagg
     8161 tggcccagcg agtcgcacga cagcgggctc acgaccggca gaaagccggc gtcgagcagg
     8221 cgggtgatga ccgagccgtc cacgtcgtcg atgtcgccca ccatcccgta gtccacggtt
     8281 tccccgctcg cgagcgtgac cggcgggcgc ttgtgggcct tgaccaggcc cgcgtcgacg
     8341 ccgctgagac cgatggcatc gatgccgaat tcgcggcaca ggccgagcag tcgcgtgttg
     8401 atgaggccgt tcaggaccat gcacgcgacg tcgatcgcgc caccgtcggt gacgcgccgc
     8461 ccctgcacca tgcgcgtctc gacgccgaac ttgtcggcga ccgtgtcgag ctgcgggccg
     8521 ccgccgtgca ccagcaccac gcggatgccg aggtggtgca ggatcgcgac ctgctccatg
     8581 aagccacgca tcgcagcgcc gtcccggaac gcgccaccgc cggtcttgat gacgaagacc
     8641 ttgcccttgt acatgtgtat gtacggcgcc gcgccccgga gcgcgcgcac ggtgacggcc
     8701 tgttcaccct tgtgtccgat cattcccgtt accccttgag caagctgtac agcaccgcca
     8761 tctgcaccgt catgcgattg gcggcttcct ggaccacgcg gctgcgcggc ccgtcgagga
     8821 cctcgtcggc gacggcgacg ttgcgccgca ccggcaggca gtgcatgaaa tggcagtcgg
     8881 cgcgcgcgga gcggaaccag tcctcgcgga cctgccacgc cgacaggggc gcgcgcagcg
     8941 ccgcctcggc ttccgcgtcg ccgtagcact gcgcggagcc ccattccttg gcgtagagca
     9001 cgtgagcgcc ggcgagtgcc gactcgcggt cgctcgtctc cgtcacctgg ccgcccgagc
     9061 gggccgcggc ggcgcgcgcc ttggccatca gtgactcggg cagtgaatag ccgtccggtc
     9121 gcagtaccac gacctccatg ccgcgttgcg cggccatgtg gaccgtcgcg gcaggtaccg
     9181 cgagcggcaa ggcgcgcggg tgattgaccc agctcagcac gaacttggcc gtccgcggca
     9241 ccgcgaggtc gtccatcgtc ttccagtcgg ccagcgcctg gcagggatgg ttcgtggccg
     9301 attccatgtt cacgaacggc ttgtcgcaca gccccgccat ggagaggaac tgggtctccg
     9361 cgaggtcagc tgcaaggtcc ttgccctcgg caaacgatcg gatgccgagc gcgtcgcaat
     9421 acgaggagag caccgggatc gcctcgcgca cgtgctcggc ggcgccggcg ttcatcaccg
     9481 caccgacgcg cgtctcgaac tgccacgtgc cctgcccggg agtgaccacg aacgacctcc
     9541 cgccgagccg catcatgccg gcctggaacg acgcgagcgt cctgagcgac ggattcatga
     9601 acagcagccc gaggatcttg ccggaaagcg cgtccggctc cggtcgctcc tcgagccggc
     9661 gtgcgagcgc gacgaggtcc tgcacctggt cacgggtgaa gtcggcgagg tcgaggaatc
     9721 gctgcatggg cttgtgacga atggcgttcg ggtgggaggt gttcagcggt cggcgcgggc
     9781 aaggctcacg gcgtggtgcg ccgctcaggc ggggagcgag gcgagcacgt cacgcaatgt
     9841 cgcgacgtgg ctttcctcg
//
LOCUS       DAOMFD010000070         9680 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2065_length_9680_cov_34.352416, whole genome shotgun sequence.
ACCESSION   DAOMFD010000070 DAOMFD010000000
VERSION     DAOMFD010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9680)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9680
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2065_length_9680_cov_34.352416"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..341
                     /locus_tag="PL152_05765"
     CDS             <1..341
                     /locus_tag="PL152_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR48820.1"
                     /translation="LATIVNFAVGLAALVAVAVASGARVTSGTTGPVPAWAWLGGLLG
                     ASYVASTTVLGPRLGAAALLALTLAGQMIAALAVDQYGVLGFPQNPVTPARLAGAVLL
                     VVGALLVIRR"
     gene            354..935
                     /locus_tag="PL152_05770"
     CDS             354..935
                     /locus_tag="PL152_05770"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HQR48821.1"
                     /translation="MARTPSTMLALGTQAPAFDLPDFDGKRHALGDYAGHPALLVAFI
                     CNHCPFVRHMRGEFASFAREYSQRGLAIVAIVSNDLDAYPQDGPDAMREEARTLGYVF
                     PYLVDADQQVAKAYHAACTPDFFLFDAERRLVYRGQFDGSRPGNAVPVTGADLRKAVD
                     AVLAGAPVDPDQRPSLGCNIKWKAGAAPDYFAG"
     gene            complement(920..1516)
                     /locus_tag="PL152_05775"
     CDS             complement(920..1516)
                     /locus_tag="PL152_05775"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQR48822.1"
                     /translation="MQSQRRFFVLLLALTTGCATTWQVDTYEAPGAGIESHRTFAWNG
                     GELGTVAAVNPQLAADVDRHVREAVVAGLVRKGYQEVTDPKAAQMLVSYQVAGTRKYV
                     TSEKPRFNAPLPDDVLRPTGPQPPAASELPPEQRVTEGSVIVFVDDPATGRLIWRGAI
                     TAETRTSSSKQGIDTVAEMAANIIESFPKAGGATQPAK"
     gene            1662..2102
                     /locus_tag="PL152_05780"
     CDS             1662..2102
                     /locus_tag="PL152_05780"
                     /inference="COORDINATES: protein motif:HMM:NF021621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host attachment protein"
                     /protein_id="HQR48823.1"
                     /translation="MSRYRILVADQAEAIFYDAPSLDAAPMEVARISDPLAHLHDRDF
                     ATERPGRTRESYGTARHAIQREDSPRREEAARFARRIARRLDDARRRDEFESLVVVAG
                     LPFLGLVRAELTKPTRARVVREIHKDLVHSPPGVLARQLAASAP"
     gene            2276..4921
                     /locus_tag="PL152_05785"
     CDS             2276..4921
                     /locus_tag="PL152_05785"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HQR48824.1"
                     /translation="MSTILLAYERDQDLTAIETLLQSRGYRVLKARNGLEALEVARSE
                     TPRAVVSDVLLPLLDGFALCRRLKEEAPTRHLPVILLSFRVEGPKYEAFAAEVGALRF
                     FPRGSTLEELAGALDSLKTGSDTMRIPALVPELIDRQQQDRRRVAELERQVRELQYSN
                     KLLATAEKLARGKVEREARERADQAAAGSDAARELQQRIEELEAKLRAQATVESDKVR
                     ELQQRLQELEAQHQELSAAETRARDAAEESRAGLARVAVLEGRLSELQSSRARAQAAA
                     ADAERAFGAQPLPTLLSDMETLEIRAVSDSAAALFGFAPQELIGRPLADLLPGATPGD
                     AGALPAELDHARPDGTRSVFQVRRASVSYAGRACWVTSLVDITAERTLRDAEQLARVG
                     SAALEAAPTAACIVDPEGRITYANAAFHALVGLEAGKAAGLPLQQFETGEGEASAAGD
                     AAPAAGATRRTTWRRPDRPGIDVEVASAAIGDAPGARIVAARDLAALRSVEERAARDR
                     QLDAGLLDLAQHTHMLTETEVPARALELARNATGAEAGYVFLSIADNAQLDLAAHSAA
                     GQDHGQTLPVRWRGAVPAGTALAECVASLRVVERESAEGTGGLRQAGLPGSLHRQLLT
                     PIMDGGRLAGVVLLANKPAPFDGEDRRRAALVADGLWKALRRRRSDAEVVSAMDHMER
                     VMLGTIEAIGALSEAQDGCKAGRARRVGEMAASIGTAMGLPGHSVRGLRVIGQLIDVG
                     MLHIPREILWRTAPLSPTEFDLVRTHAERGYEILRRIDFPWPVAEAVRQHHERHDGSG
                     YPRGLKGEEIMIEARIVAVADAVEAMLSPRPQRQALSMSACIDELQAQAGRRYDAAAA
                     RACVRLLRDRQTQAEPEARAGQRIA"
     gene            4929..6335
                     /locus_tag="PL152_05790"
     CDS             4929..6335
                     /locus_tag="PL152_05790"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR48825.1"
                     /translation="MRLQFFGAAGEVTGSCHILEVGGRTLLLDCGLIQGGPTHAARNR
                     EPFPFDPARIDAVVLSHAHIDHCGRLPLLHKRGFRGPVHATPACRDLARILLHDCATM
                     AERDAEREQRRRQRNGGRGTVEPLFTTGDADAVLQAFRTVPYGRTIDVAPGVEVTFRD
                     AGHILGSASVCLRISEQGLERRVLFSGDVGQYGSPILRDPEAGLAADVVLMESTYGGR
                     LHRDRDATLAEFGEILRQARRDGGNVLIPAFAVGRSQDILYELGTHYDAWQLADWQVF
                     LDSPMAIEASQVYWRHVELYDEEARELRQRAGAMPPLPNLTLCRTVEESMAINRITDG
                     AIVIAGSGMCTGGRIVHHLKHNLSRRQCDVVFTGYQAVGTLGRAIVDGREEVHVNGVP
                     VRVEARIHTLGGFSAHGDQADLLRWYTGVTGRPPVWLVHGERESAGALRDALAERGVR
                     ATVAEPGMRLDLATLSPG"
     gene            complement(6358..8025)
                     /locus_tag="PL152_05795"
     CDS             complement(6358..8025)
                     /locus_tag="PL152_05795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR48826.1"
                     /translation="MPAPATPRQALLVAEPRDAAVLVDVLVLSTDVPLFDAIRGAIGE
                     RNPVWRARTAEESVDLLLTGRCGVLLVDMAAVSTNPTTLIAQIVEQFPDVIVVVAGRR
                     EDEALLAHLITEGLVYRFMHKPLTAKRAGMFLQAAIRRHVEQRESQVNQALLPIPRVL
                     PGRFDPGKWLFVAAGVAMFVAALVLLAGGRPPRGQDAEGAATQIVTPAVVSTAPQADP
                     VLSRARAAFAAGRFEAPAGRNALDLYAAVLLSRPDLPEARTGLDSTIDRIVSVARLAA
                     ADGNLPEARRLLSRVEAVAPSSLAVQRLAMYLAPQSPPSGTDTPEALAAASSAAAVVA
                     TAPAPLPAPLPAAPRRDVQSSPSAGQATARQQPLTGQAADRAATPQALHPRPVVTPVV
                     VTPDPLAPRVANADELRSTASRSSRVATRTFGAPISSGLPTAGYVKAPRPEPAPVEKP
                     GTSLDPATTAALAMPTDELGRIHATDPVYPADALRNHVEGWVELSFTITETGSVRDVE
                     VVDSQPHGVFDTAATEAVEGWRFRPRLANGQPVAHRSYVTLRFNVEG"
     gene            8252..8698
                     /locus_tag="PL152_05800"
     CDS             8252..8698
                     /locus_tag="PL152_05800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48827.1"
                     /translation="MRTPVGYTSRISARHRAALERLLFFNGCQSRVAHGIVDAIDKYG
                     PPEILADAGWLRVKVAGLPDVQSLFAVDEISGEPLGVAVYTRADLEHITVLHVGLDEK
                     YCAGGERESAKLLLRLVKEIRRSSKRVKGVRRLSVHYGTQRTRAQA"
     gene            8733..>9680
                     /locus_tag="PL152_05805"
     CDS             8733..>9680
                     /locus_tag="PL152_05805"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HQR48828.1"
                     /translation="MRRDSTQRVPASAATRLLLAALTALGVASALALAAPIGWPWELF
                     VHFRVQYAAGALAVAVALALCRHPRACMVGIALAAFNALPLLQRAQAGSSAGDCAGRE
                     FTVVSANLQAGNDDRRFLDWLAGHPADLVVVQEVTEAWARDLSRLPDYPHRILLTRED
                     PYGIGVLSRWPLDAVQQEDLAADGLPSLAGRVQFEGRSLRWLALHTHWPITPALARAR
                     DRSLDAAAALVRGSDGPIVVLGDFNATPFAPAYADFVRISGLRDASATARWQPTWMAG
                     FWPLALRIDYVFASPSLCVESSSVGSAIGSDHRPVLAKLR"
BASE COUNT         1409 a         3364 c         3516 g         1391 t
ORIGIN      
        1 tcctcgcgac gatcgtcaac ttcgccgtcg ggctggcggc gctggtcgcc gtcgccgtcg
       61 cgagcggcgc gcgcgtgacg tccgggacca cgggcccggt gcctgcgtgg gcgtggctcg
      121 gcggccttct cggtgccagc tatgtcgcct cgaccaccgt gctcgggccg cgactcgggg
      181 ccgctgcctt gctcgccctg acgctcgcgg ggcagatgat cgcggcactg gcggtcgacc
      241 agtacggcgt gctgggcttt ccgcagaacc cggtcacgcc ggcgcggctg gcgggagccg
      301 tgctgctggt cgtgggtgcg ctgctggtca ttcgtcgcta ggaggtcccg gccatggccc
      361 gcacaccctc caccatgctg gcgctcggta cgcaggcgcc cgccttcgac ctgccggact
      421 tcgacggcaa gcgccacgcg ctcggcgact acgcgggtca cccggctctg ctcgtggcgt
      481 tcatctgcaa ccactgcccg ttcgtccggc acatgcgcgg cgaattcgcg agcttcgcgc
      541 gcgagtactc gcagcgcggg ctcgccatcg tcgccatcgt ctcgaacgat ctcgatgcct
      601 atccgcagga cggtcccgac gccatgcgcg aagaggcgag gacgctgggc tacgtgtttc
      661 cgtacctcgt cgacgcggac cagcaggtcg ccaaggcgta ccacgcggcc tgtacaccgg
      721 atttcttcct gttcgacgcc gaacgacgcc tcgtgtaccg cggccagttc gacggcagcc
      781 ggccgggcaa cgccgtgccg gtgaccgggg cggacctgcg caaggcggtc gatgcggtgc
      841 tggcaggcgc gcccgtcgat cccgatcagc gaccgagtct cggctgcaac atcaagtgga
      901 aggccggcgc cgcgccggac tatttcgccg gctgagttgc gccgccggcc ttcgggaagg
      961 actcgatgat gttggccgcc atttccgcca ccgtgtcgat gccctgtttc gaggacgacg
     1021 tccgggtctc ggcggtgatc gctccacgcc agatgaggcg gcctgtcgcg gggtcgtcca
     1081 cgaacacgat caccgacccc tcggtcacgc gttgctcggg cggcaactcg gaagcggcgg
     1141 gtggctgggg accggtgggc cgcagcacgt catcgggcaa cggggcgttg aaccgcggtt
     1201 tctcgctggt cacgtacttg cgcgtgccgg ccacctggta gctcacgagc atctgggcgg
     1261 ccttcgggtc cgtcacctcc tggtagccct tgcggaccag tcccgcgacc acggcctcgc
     1321 ggacgtggcg gtccacgtcg gcggcgagct gcggattgac ggcagccacc gtgccgagtt
     1381 cgccaccgtt ccacgcgaac gtgcggtgcg actcgatccc ggcgcccggc gcctcgtacg
     1441 tgtccacctg ccacgtcgtg gcgcatcccg tcgtcagggc cagcaacagg acgaagaagc
     1501 gccgctgaga ctgcatgtcg tgctcccttc ttgtgccaac ctgtataggg accacggcga
     1561 cccgctgtca acctcccgta tccgcatgcc acattccacc attgcacgag tggacacgcc
     1621 ggaagagtct agtgacaggc cccactgcag gggaggttcc catgagcagg tatcggatcc
     1681 tcgttgccga ccaggccgaa gcgatcttct acgacgcacc gtcactcgat gcggcaccga
     1741 tggaggtggc gcggatcagc gatccgctcg cgcacctgca cgaccgcgat ttcgcgaccg
     1801 agcggccggg acggacgcgg gaaagctacg gtacggcgcg ccatgcgatc cagcgcgagg
     1861 acagcccgcg ccgcgaggaa gcggcacggt tcgcacggcg catcgcacgc cgcctcgatg
     1921 atgcacgccg ccgcgacgag ttcgagtcgc tcgtcgtcgt ggccggactt cccttcctcg
     1981 gcctggtgag ggctgagctg acgaagccga cccgggcgcg cgtcgtccgt gagatccaca
     2041 aggacctggt gcacagcccg cccggcgtgc tggcccggca gctggccgcc agcgcaccgt
     2101 gacacggggc acgttcggca cgggcattct gtgaccggct tcccgcggta gcgcgccagc
     2161 ttggtgcccg cgccgaggcc gggcatactt acgccgtcag gcaaggcggg gccatgagac
     2221 ggcgcatgca cagtgcgggc ggctgcttac tccaggcgca gggcgtcgtg cgcccatgag
     2281 caccatcctg ctcgcttacg agcgtgacca ggacctcacg gcgatcgaga cgctgctgca
     2341 gtcgcgcggc taccgcgtac tgaaggcacg caacggcctc gaggcgctgg aagtcgcgcg
     2401 cagcgagaca ccgcgcgccg tggtgagcga cgtgctcctg cccctgctcg acggcttcgc
     2461 gctctgccga cgactgaagg aagaggcgcc gacgcgccac ctgccggtga tccttctctc
     2521 gttccgggtc gaaggcccga agtacgaggc gttcgcggcg gaagtcgggg ccctgagatt
     2581 cttcccgcgc ggctcgacgc tcgaggagct cgccggcgcg ctcgactcgc tcaagaccgg
     2641 ctccgacacc atgcgcatcc cggcgctggt gccggaactg atcgaccggc agcagcagga
     2701 ccggcgacgc gtggcggaac tggagcgcca ggtccgtgag ctgcagtaca gcaacaagct
     2761 gctggccacg gccgaaaagc tcgcgcgcgg caaagtcgag cgtgaggccc gcgagcgggc
     2821 cgatcaggcc gccgcgggct cggacgcggc acgcgagctg cagcagcgca tcgaggagct
     2881 ggaagcgaag ctgcgcgcgc aggcgaccgt ggagtccgac aaggtccgcg aactgcagca
     2941 gcgcctgcag gaactggagg cacagcacca ggaactgtct gcggccgaga cgcgcgcgcg
     3001 tgacgcggcc gaagagtcgc gtgcgggcct cgccagagtc gctgtcctgg aaggacgcct
     3061 gtccgagctg cagtcgagcc gcgcgcgcgc gcaggcggct gcggcggacg cggagcgggc
     3121 cttcggcgcg caaccactcc cgacactgct ttccgacatg gagacgctgg agatccgcgc
     3181 ggtcagcgat tcggccgcgg cgctgttcgg gttcgcgccg caggaactca tcggccggcc
     3241 gctggccgac ctgctgcccg gcgccacgcc gggggacgcc ggcgcgctgc cggcggaact
     3301 cgatcacgcc aggccagacg ggacgcgatc cgtgttccag gtgcggcgcg cgtcggtctc
     3361 ctatgcaggg cgggcgtgct gggtgacgtc cctggtggac atcaccgccg agcgaaccct
     3421 gcgcgatgcc gagcagctcg cgagggtggg atccgcagcg ctcgaggccg ccccgacggc
     3481 ggcgtgcatc gtggatccgg aaggcaggat cacgtacgcg aacgcggcgt tccacgcgct
     3541 cgtggggctc gaggccggca aggccgcggg cctgcctctg cagcagttcg agaccggcga
     3601 aggcgaggca tcggctgccg gcgacgccgc gcccgccgcg ggcgcgaccc gcaggaccac
     3661 ctggcgcagg ccggaccggc ccggcatcga cgtcgaggtt gcgagtgccg cgatcggcga
     3721 cgcgccgggc gcacgcatcg tcgccgcgcg cgaccttgcg gcgttgcgca gcgtcgagga
     3781 acgtgcggcg cgggacaggc agctcgacgc gggactgctc gacctggcgc agcacacgca
     3841 catgctgacc gagaccgaag tgccggcccg cgcgctcgag ctcgcgcgca atgcgaccgg
     3901 tgccgaggcc ggttatgtat tcctgtcgat cgcggacaac gcgcagctgg atctcgccgc
     3961 gcacagcgcg gcgggccagg atcatggaca gacgctgccg gtgcgctggc gtggagcggt
     4021 gccggcgggc acggcactgg ctgaatgcgt ggcatcgctg cgggtcgtcg agcgcgagtc
     4081 ggccgaaggc acgggcggcc tgcgacaggc cggcctgccg gggtcgctgc accggcagct
     4141 cctcacgccg atcatggacg gcggacggct cgcgggcgtg gtcctgctcg ccaacaagcc
     4201 cgcaccgttc gacggcgagg accgcaggcg ggcggcgctg gtcgccgacg ggctgtggaa
     4261 ggcattgcgc cgacgccgct ccgacgccga ggtcgtgagc gccatggacc acatggagcg
     4321 cgtgatgctc ggcacgatcg aggcgatcgg tgcgttgtcc gaggcgcagg acggctgcaa
     4381 ggcgggtcgc gcgcggcgcg tgggcgagat ggccgccagc atcggcacgg ccatgggcct
     4441 gcccggccac agcgtgcgcg gcctgcgcgt gatcgggcag ttgatcgacg tcggcatgct
     4501 gcacatcccg cgcgagatcc tctggcgcac cgcgccgctc tccccgacgg aattcgacct
     4561 cgtccggacc cacgcggagc ggggctacga gatcctgcgc cgcatcgact tcccgtggcc
     4621 ggtcgccgaa gcggtgcggc agcatcacga gcgccacgac ggctcgggct atccgcgcgg
     4681 cctcaagggc gaagagatca tgatcgaggc gcggatcgtc gcggtcgcgg atgcggtcga
     4741 ggcgatgctg tcgccgcggc cgcaacggca ggccttgtcg atgtcggcct gcatcgacga
     4801 gctgcaggcc caggcgggcc gtcggtacga cgcggccgcc gcgcgcgcct gcgtgcggct
     4861 gctgcgggac cgccagacgc aggcagaacc ggaggcccgg gccggacagc gcatcgcctg
     4921 aggcagtgat gcgcctgcag ttcttcggcg ctgcgggtga ggtgacgggc tcctgccaca
     4981 tcctcgaggt cggcggccgc acgctgctgc tcgactgcgg gctcatccag ggcgggccca
     5041 cgcacgccgc acgcaatcgc gagccctttc cgttcgaccc ggcgcgcatc gacgccgtcg
     5101 tcctgagcca cgcgcacatc gaccactgcg gccggttgcc gctgctgcac aagcgcggat
     5161 tccgcgggcc cgtgcacgcg accccggcgt gccgcgacct cgcgcgcatc ctgctgcacg
     5221 attgcgccac gatggcggag cgcgacgcgg aaagggagca gcgcaggcga cagcgcaacg
     5281 ggggccgtgg gacggtcgag ccgctgttca cgacgggcga tgccgatgcc gtcctgcagg
     5341 cattccggac cgtgccttac ggccgcacga tcgacgtcgc acccggcgtc gaagtgactt
     5401 tccgggacgc gggacacatc ctcggctcgg ccagcgtctg cctgcgcatc agcgagcagg
     5461 gactggaacg gcgcgtgctg ttcagcggcg atgtcggcca gtacggctcg ccgatcctgc
     5521 gggacccgga agccgggctc gccgccgacg tggtcctgat ggagagcacg tacggcggcc
     5581 gtctccatcg cgatcgcgac gccacgctcg ccgaattcgg cgaaatcctg cgccaggcgc
     5641 gccgcgacgg cggcaacgtg ctgatcccgg cgttcgcagt cgggcgcagc caggacattc
     5701 tctacgagct cggcacgcac tacgacgcgt ggcagctcgc cgactggcag gtcttcctcg
     5761 acagcccgat ggcgatcgag gcgtcgcagg tgtactggcg gcacgtcgag ctctacgacg
     5821 aggaggcgcg cgagcttcgc cagcgcgccg gcgcgatgcc gccgctgccg aacctgaccc
     5881 tgtgccgtac ggtggaagaa tccatggcga tcaaccggat caccgacggt gcgatcgtga
     5941 tcgcgggcag cggcatgtgc accggcggcc gcatcgtgca ccacctgaag cacaacctgt
     6001 cgcgccggca gtgcgacgtg gtgttcaccg gttaccaggc cgtgggcacg ctcgggcgcg
     6061 ccatcgtgga cggtcgcgag gaagtccacg tcaatggcgt gccggtgcgg gtcgaggcgc
     6121 ggattcacac gctgggcgga ttttccgccc acggcgacca ggccgacctg ctgcgctggt
     6181 acacgggcgt gacggggcgc ccaccggtct ggctggtgca cggcgagcgc gagtccgccg
     6241 gcgcgctgcg cgacgcactt gccgaacgtg gtgtacgcgc gacggtcgca gagcccggga
     6301 tgcgcctgga cctggcgacg ctgtcgccag ggtagctgcc gcggcgtcgg ttcgccgtca
     6361 gccctcgacg ttgaatctca gcgtgacgta ggaacgatgt gcgaccggct ggccgttcgc
     6421 cagccgcgga cggaatcgcc agccttccac cgcctcggtt gccgccgtat cgaagacacc
     6481 atgcggctgc gaatcgacga cctcgacatc ccgtaccgat ccggtctcgg tgatggtgaa
     6541 cgagagttcg acccaaccct cgacgtggtt gcgcagcgcg tccgccggat agaccgggtc
     6601 ggtcgcgtgg atgcggccga gttcgtcggt cggcatggcg agcgctgcag tggtcgcggg
     6661 atccagggat gtcccgggct tttccacagg ggcaggctcg ggccgaggcg ccttgacgta
     6721 gcccgccgtc ggcagtccgg aggagatcgg cgcaccgaac gtccgcgtcg ccacgcggct
     6781 cgagcgcgaa gcggtcgacc gcagttcgtc cgcattcgcg acccggggcg cgagtggatc
     6841 cggtgtgacg acgaccggcg tcaccacggg acgcggatgc aaagcctgcg gcgtggctgc
     6901 gcggtccgca gcctggcccg tcagcggctg ctggcgtgcc gtggcctggc ctgcactcgg
     6961 agaggactgc acgtcgcggc gcggagctgc gggcagcggc gcgggaagcg gtgcgggcgc
     7021 cgtcgcgacc acggctgcgg ccgaagaggc cgctgccagg gcttcagggg tgtccgtgcc
     7081 ggacgggggc gattgcggcg cgaggtacat tgccagtcgc tgaaccgcga gactggacgg
     7141 tgcgacggcc tcgacacgcg agagcagcct gcgggcctcc ggcaggttgc cgtcggcggc
     7201 cgcgagacgc gcgacgctca cgatccggtc gatggtcgag tcgagaccgg tgcgtgcctc
     7261 gggcaggtcc ggccgggaca gcaggaccgc ggcgtagagg tcgagcgcat tgcgccccgc
     7321 cggcgcctcg aagcgacccg cggcgaaagc cgctcgtgca cgcgacagta ccgggtccgc
     7381 ctgtggcgcg gtcgacacga cggcgggcgt cacgatctgc gttgcggcgc cctctgcgtc
     7441 ctgcccgcgc gggggccggc cgccggccag caggaccagc gcggcgacga acatcgccac
     7501 gcctgccgcg acgaacaacc acttgcccgg gtcgaaccga cccggcagga ccctcgggat
     7561 gggcagcagc gcctggttca cctgcgattc gcgctgttcg acgtgacgtc gtatggccgc
     7621 ctgcaggaac atgcccgctc gcttcgccgt gagcggcttg tgcatgaagc ggtagacgag
     7681 gccctcggtg atcaggtgtg cgagcagcgc ctcgtcctcg cgccgtcccg cgaccacgac
     7741 gatcacgtcc ggaaactgct cgacgatctg cgcgatcagc gtcgtgggat tggtcgagac
     7801 ggctgccatg tcgacgagca gcacgccgca gcggccggtc aggagcaggt cgaccgactc
     7861 ctccgctgtt cgcgcccgcc agaccggatt gcgctcgccg atggcgccgc gaatggcgtc
     7921 gaacagcggc acgtccgtcg acagcacgag cacgtcgacg aggaccgccg cgtcgcgcgg
     7981 ctcggcgacg agcaacgcct ggcgtggtgt ggcaggggct ggcaccccgc gattccgcgg
     8041 cagaaggccc gaaacgtcca ccgttgacgg gcaaaaatct gaagcaattc aaacttctgt
     8101 cgcctgccgc aggggtcggc caggcggagg cccggctggc gtatcgagac tgggtccagc
     8161 cggggtctgc cgccactcga caggtcgcag ccgtgcgagc atgatcgccg ctcgtccccg
     8221 agaagaccgg tgcaacggtc caggcccagg catgaggaca ccggtcggat acacctcgcg
     8281 gatatctgcc aggcatcgcg ccgcgctcga gcggctgttg ttcttcaacg gctgccagag
     8341 ccgggtcgcg cacggcatcg tcgacgccat cgacaagtac gggccgcccg agatcttggc
     8401 cgacgcgggc tggctcaggg tcaaggtggc gggactgccg gacgtgcagt ccctgttcgc
     8461 agtggacgaa atcagcggcg agccactcgg tgtcgccgtc tacacgcgcg cggacctcga
     8521 gcacatcacg gtgctgcacg tgggcctgga cgagaagtac tgcgccggcg gcgagcgtga
     8581 gagcgccaaa ctgctgctca ggctggtcaa ggaaatccgg cgttcgtcga agcgcgtcaa
     8641 gggagtgcgc cggctgagcg tgcactacgg gacgcagcgg acccgcgcgc aggcctgatc
     8701 gagacccgac ggcacgccac ctgtcgccga ccgtgcgacg agactccacg cagcgagttc
     8761 cagcatctgc cgccacccgg ctgctgctcg cggcactcac ggcactcggc gtcgcgagcg
     8821 cgctggcgct tgccgctccg atcggctggc catgggaact cttcgtgcac ttccgcgtgc
     8881 agtacgccgc gggcgccctt gcggtcgccg tcgcactcgc gctgtgtcgc catccgcggg
     8941 cgtgcatggt tggcattgcg ctggcggcgt tcaacgcgtt gcccctcctg cagcgcgcgc
     9001 aggcgggctc ctcggccggc gactgcgcgg gacgggaatt cacggtcgtg tccgcaaacc
     9061 tgcaggcggg caacgacgac cggagattcc tcgactggct cgccgggcac ccggcggatc
     9121 tcgtcgtcgt gcaggaggtg accgaggcct gggcgcgcga cctgtcgcga ctcccggact
     9181 atccgcaccg catcctcttg acccgggagg acccctacgg catcggcgtg ctgagccggt
     9241 ggccgctcga cgcggttcag caggaggacc tggcggccga cggactgccc tcgctggcgg
     9301 gtcgcgtgca gttcgaggga cggtcgctgc gatggctcgc cctgcatacg cactggccga
     9361 tcacgcctgc gctggcgcgc gctcgcgacc ggtcgctcga tgccgccgcg gcgctggttc
     9421 gcggttccga cgggccgatc gtcgtgctcg gcgatttcaa tgcgacgccg tttgccccgg
     9481 cctatgcgga cttcgtgcgg atttccgggc tgcgcgacgc gtccgccacc gcgcgctggc
     9541 agccgacctg gatggcgggc ttctggccgc tggcgctcag gatcgactat gtgttcgcgt
     9601 cgcccagcct ttgcgtggag agttcgagcg tcggttccgc gatcggctcg gatcaccggc
     9661 cggtgctggc gaagctgcgg
//
LOCUS       DAOMFD010000071         9585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2096_length_9585_cov_34.370304, whole genome shotgun sequence.
ACCESSION   DAOMFD010000071 DAOMFD010000000
VERSION     DAOMFD010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2096_length_9585_cov_34.370304"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(16..141)
                     /locus_tag="PL152_05810"
     CDS             complement(16..141)
                     /locus_tag="PL152_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48829.1"
                     /translation="MARPPNYSQDKKRREEAARKKREEKEQRRRQRKDGPPPPTA"
     gene            487..1233
                     /locus_tag="PL152_05815"
     CDS             487..1233
                     /locus_tag="PL152_05815"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HQR48830.1"
                     /translation="MRILGITNIKGGVGKTTTAVNVAYLAAESGRATVLWDLDPQGAA
                     TYTLCAEPAERASAKKLVRGKQELPELTTPTAYPALNVIPADFSYRNLDVHLSERKHA
                     TERLMMMTRSLYDEIDLLVLDCPPGMTLLTENVLRAADALVVPIVPAPLSLRMLDQMH
                     AFIAHTGWSDLQILPFFSMVDRRKSLHHEVIAKARADHAELLATEIPYWSDIERMTVR
                     RAPLPAFAPSSPAARLYAQLWQEIAERLFE"
     gene            complement(1279..3504)
                     /gene="katG"
                     /locus_tag="PL152_05820"
     CDS             complement(1279..3504)
                     /gene="katG"
                     /locus_tag="PL152_05820"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HQR48831.1"
                     /translation="MQRKRMTSFVACAIAALPLASTAALGASGAPMPNQFWWPEQLDL
                     SVLRQHDARSNPLGADFDYARAFSSLDLEAVKKDIKQALTTSQDWWPADYGNYGPFFI
                     RMAWHSAGTYRTIDGRGGADGGQQRFEPLNSWPDNANLDKARRLLWPVKQKYGASLSW
                     GDLMVLAGNVSLESMGFKTFGFAGGRADDWEPDLVYWGPEKKMGADERYSGNRQLQKP
                     LAAVQMGLIYVNPEGPNGNPDPLAAARDIRETFGRMAMNDEETVALIAGGHTFGKAHG
                     AHDPAKCVGPDPAAAPIEQQGFGWANKCGRGNGPDTVTSGLEGAWSANPVAWTTQYLD
                     NLFAFEWVQTRSPGGAIQWIPANGQASSLVPDAFDPAKRHAPIMFTTDLSLKFDPSYR
                     EIAQRFQKDPQAFQLAFAKAWFKLTHRDMGPRARYLGSEVPKEVLLWQDPVPPVDHPL
                     IDANDAAALKAKILASGLTGPELVRTAWASAASYRGTDMRGGANGARIRLAPQKDWPV
                     NDPAELARVLQRLEAIQTAFNATLPGGKKVSLADTIVLGGNAAVEQAAKKAGYDVSVP
                     FVAGRTDASQAQTDVNSFAVLEPSADGFRNYFGTAAGRSPPEALVDRANMLTLTVPEM
                     TVLVGGMRALNANAGQSPHGVFTNRPGTLSNDYFVNLLDMSTKWSKSPTSEGVYEGRD
                     RKSGALKWTATPVDLVFGSNTELRAVAEVYGSSDGQERFVRDFVKAWNKVMNLDRVDV
                     K"
     gene            3814..4758
                     /locus_tag="PL152_05825"
     CDS             3814..4758
                     /locus_tag="PL152_05825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48832.1"
                     /translation="MRLSPRFLFAPMATLLLAAGSAFASAAGTACDGELARQIPARPP
                     EALTGGEFAERARDLAGPPRDAMVQGELEAGDVPAFLRHLVPVTVSDAQNSVTVCVMP
                     DYLALGTDQDFAYVPLGLEAALDVADRLGFMLPTRKLVNAIYAESTVKLDPQPLPAGD
                     AMRSTDYLVRHNTMVRAQRQSRGAALGDLTAGDKKDLVLTPRLWEVPGRVAIYGWHRA
                     VGSPIQPLSTVHGARYADYSHGIRLVSQVAYVNGVERPLVDVLADPRLASLVSDEGPM
                     PRLTERLSALRAGLRSDMAAAPTVAWLPRQGSSARAAP"
     gene            complement(4780..5793)
                     /locus_tag="PL152_05830"
     CDS             complement(4780..5793)
                     /locus_tag="PL152_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HQR48833.1"
                     /translation="MRQGHVVALIAIAVLASAAGARGDTANDEQNRALFQKLATVHGL
                     TPAQMQRIEAIFAGNFAMGQGNPAISRHPESPAQCLENLERRGIVYANPEYERICGAK
                     YMAPLYDPTTGTSARARACIDQFEFPDIPCEYPVIWVKAREAAEICAAEGKRLCDAHE
                     WEGACQGSLGPPDYRFDLAQGVSPEIAVQRMRAAHNREYAATRRWSYGPEYQRGICAT
                     GSSKTPGCQGGGWSMCGSNTYPAGSFPACRSPLDVYDLNGNAAEHMNLPLDPSQMAST
                     GSSALGVTEMKGSWFIFDTYRAHEDWCRWRAPYWHGSRVMDPHSHANYHLGFRCCKTL
                     DEK"
     gene            complement(5790..7586)
                     /locus_tag="PL152_05835"
     CDS             complement(5790..7586)
                     /locus_tag="PL152_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48834.1"
                     /translation="MGRTPRALVILGLLAAAGAAVTAGAPATLRAPALPGAAQLERQA
                     AEYRAEQPKTILELQPFRSTTTVPVRAADGRAGTATLVNLNPYANAWYLLTLEFGATG
                     QGPSYHLENPRPREIALTLLAGDPTSLRVAVGERGAPCTLWTSRPAAPLDEASRTGLP
                     YAPLCDGRLYLRNPVAGRRTSLERVADFLRDHVWGGEQLVGFVKEQVYRDAFLEKPVT
                     HEAGAAATRAAMPQAPRPAAIAPDRAGLAIIPEQLGLDLAAPGRELLVGRWYPVRDLA
                     GVYASAVTPGALEPSLLGPSRGVNALGPVELDALVYLVAFDLARFDLHFVLGTDHPRV
                     GWSDRVLPTVRDPALPGPDGIDRPAPLVVNGMVSPAEAARTIATFTGGFKRDHGAFRY
                     GALAQVNHGSHYGFVEQGVVFSKLQPGLATVLAFDDGRVELETWSAAGNSRLSGIRYA
                     RQNGVPVIEFDPAVGRGVPGALVSNWGAGNWSGSDKGDLRSLRAGVCLQSNDARRFLI
                     YGYFSDATPSAMARVFEAYGCQYALHLDMNALEHTYLALYVTRGKERLVEHLIQGMDV
                     LDQGSGTNYAPRFIGFPDNRDFFYLTRREAPP"
     gene            complement(7599..8513)
                     /locus_tag="PL152_05840"
     CDS             complement(7599..8513)
                     /locus_tag="PL152_05840"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UbiA family prenyltransferase"
                     /protein_id="HQR48835.1"
                     /translation="MPDTSSSRLLAGTSLARAALDALRPYQWLKNLLIFVPLAAAHRL
                     HEPPLLHAALRAFVAFSLCSSAVYVVNDLHDLASDRLHPYKRRRPIAAGRLPRGLAIT
                     LVPLLLAASLLVVWPLGLVAAGVLAGYFVLMLAYSLGLKDIVLLDVLVLATGYAARVL
                     LGAVAVAIVPSSWLLAFCIFFFFSLALLKRYAEISVRQVIDGAHAHARAYLVEDQSLI
                     IALGVSSGYLAVLVLALYTSTLTAGMLYRHPAVIWLTCTLLLYWISHLWLVAHRGRMP
                     DDPLRFILEDRLSWILVPLMGLTAWLAL"
     gene            complement(8494..9537)
                     /locus_tag="PL152_05845"
     CDS             complement(8494..9537)
                     /locus_tag="PL152_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR48836.1"
                     /translation="MPSPVTLVTGGAGFLGINLCRHLLGIGRRVRSIDIADFAYPERS
                     AVNVIDGDIRDPAAVERSMQDVETVVHCAAALPLAPAEEIRSTDVDGTRLLLESALTH
                     RVVRFIFISSTAVYGIPDHHPLLETDRTQGVGPYGESKIAAEALCSEFRARGLCVSVL
                     RPKTFVGPERLGVFELLYAWAREGRNFPLIGPGNNRYQLLDVADLCQAIELCMTREPG
                     AVSDTFNVGAKEFGTMRENVQAVLDRAGHGGHAIGLPRAPAVLALRALNALHLSPMYR
                     WIYETAAEDSFVSIERIERKLGYRPRYSNRDALVRNYDWYVAHCNEIRPATGTTHRVR
                     WKRGVLDLARHFF"
BASE COUNT         1462 a         3290 c         3334 g         1499 t
ORIGIN      
        1 gcgtgccgac ccaggctacg ccgtgggcgg cggcggcccg tccttcctct gccgacggcg
       61 ctgctccttt tcctcgcgct tcttgcgcgc agcttcctcg cgccgcttct tgtcctggga
      121 ataattgggg ggtctggcca tgcgctttcc gggttcctgc ggtgtgtccc gcgccgtatc
      181 ttagtgcagt gccgcggtgt cggcagcaat acagcgactt cggaccggcc accccgtcat
      241 gtcacgcgcg cccggccgca agggatcttg ctcttgccat gggcgcgcgt agggcgtcct
      301 atggggcggt ccgattccac atcatcgagc gacgccggga aatgcgggac aaatacccga
      361 tcgacgccag tgacccgcaa cgcaggccgg ctgctggtgt ggccgcggag cgccgcaagt
      421 ccttgaagac gctcgccgtc tgtgcgtggt cgccgctgcg gcgttcgact cgttgaccgg
      481 caccgtatgc ggatcctcgg catcacgaac atcaagggcg gtgtcggcaa gaccaccacg
      541 gccgtgaatg tcgcgtacct cgccgcggag tccggccgcg cgacggtcct gtgggacctc
      601 gatccgcagg gagcagcgac gtacacgctg tgcgcggagc cggctgaacg cgcctcggcg
      661 aagaagctcg tgcgaggcaa gcaggaactg ccggagctca ccacgccgac ggcgtacccg
      721 gcgcttaacg tgatcccggc cgacttctcc taccggaacc tcgacgtgca cctgagcgag
      781 cgcaagcacg cgaccgagcg tctcatgatg atgacccgct cgctgtacga cgagatcgac
      841 ctcctggtcc tcgactgccc gccgggaatg acgctgctca cggaaaatgt cctgcgcgcc
      901 gccgacgcgc tggtcgtgcc catcgtgccg gcgccacttt cgctgcgcat gctcgaccag
      961 atgcacgcgt tcatcgccca caccgggtgg agcgacctgc agatcctgcc gttcttctcg
     1021 atggtggacc ggcgcaagtc gctgcatcac gaggtgatcg caaaggcgcg ggctgaccat
     1081 gcggagctgc tcgccaccga gattccgtac tggagcgaca tcgagcgcat gaccgtccgt
     1141 cgggcaccgt tgccggcctt cgcgccgtcc agcccggcgg cccgcttgta cgcacagctc
     1201 tggcaggaaa tcgcggagcg actcttcgag tagcgggcgg tacgcgcccc gggatccgag
     1261 gcgcgtaccg caccaggatc acttcacgtc gacgcgatcg aggttcatca ccttgttcca
     1321 cgccttcacg aagtcccgta cgaaccgctc ctgcccgtcg gacgagccgt agacctccgc
     1381 cactgcgcgc agctccgtgt tcgagccgaa gaccaggtcg acgggagtcg ccgtccactt
     1441 gagcgcgccg ctcttgcggt cacgcccttc gtagactccc tcggacgtgg gcgacttgct
     1501 ccacttcgtg gacatgtcga gcaggttcac gaagtagtcg ttgctcagcg tcccgggccg
     1561 gttcgtgaac acgccgtgcg gcgactgtcc cgcattggca ttcaacgccc gcatgccgcc
     1621 gacgaggacc gtcatctcgg ggacggtcag cgtgagcatg ttcgcccggt cgaccagcgc
     1681 ctccgggggc gagcgacccg cggcggtgcc gaagtagttg cggaatccgt ccgcggaggg
     1741 ctcgagcacg gcgaacgagt tgacgtcggt ctgcgcctgc gaggcgtccg tgcggccggc
     1801 cacgaaggga accgacacgt cgtagccggc cttcttcgcg gcctgctcga ccgcggcatt
     1861 gccgcccagc acgatcgtgt ccgcgagcga caccttcttg ccaccgggca gcgttgcgtt
     1921 gaacgcggtc tggatggcct cgaggcgctg cagaacgcgc gccagctcgg ccggatcgtt
     1981 gaccggccag tccttctgcg gtgcgaggcg gatgcgcgca ccgttggcac cgccgcgcat
     2041 gtcggtgccg cggtacgagg ccgccgacgc ccaggccgtc cgcacgagct cggggccggt
     2101 gaggcccgag gcgaggatct tcgccttcag cgcagccgcg tcattcgcgt cgatcagcgg
     2161 gtggtcgacc ggcgggaccg gatcctgcca gagcaacact tccttcggca cctcggagcc
     2221 gaggtaccgg gctcgcggtc ccatgtcgcg gtgcgtgagc ttgaaccagg ccttggcgaa
     2281 cgcgagctgg aacgcctgcg ggtccttctg gaagcgctgc gcgatctcgc gatagctcgg
     2341 gtcgaatttc agcgaaaggt ccgtggtgaa catgatcggc gcatgccgct tcgccggatc
     2401 gaacgcatcc gggaccaggc tggacgcctg gccgttcgcg ggaatccact ggatcgcgcc
     2461 gccggggctg cgcgtctgga cccactcgaa ggcgaacagg ttgtcgaggt actgcgtcgt
     2521 ccatgcgacc gggttcgccg accacgcgcc ctcgagaccg ctggtgaccg tgtcggggcc
     2581 gttgcctcgg ccgcacttgt tggcccagcc gaagccttgt tgctcgatgg gggccgcggc
     2641 cggatccggt cccacgcact tggccgggtc gtgcgcgccg tgcgccttgc cgaaggtgtg
     2701 gccgccggcg atcagcgcca ccgtctcctc gtcgttcatg gccatgcgac cgaaggtctc
     2761 gcggatgtcc ctcgccgccg ccagcggatc cggattgccg ttcgggccct ccgggttgac
     2821 gtagatcagg cccatctgca cggcagccag cggtttctgc agctggcggt tgccgctgta
     2881 acgctcgtcg gcgcccatct tcttttccgg gccccagtag acgaggtcgg gttcccagtc
     2941 gtccgcacgt ccgcccgcga agccgaaggt cttgaagccc atcgactcca gcgacacgtt
     3001 gccggcaagc accatcaggt cgccccacga caggctcgcc ccgtacttct gcttgaccgg
     3061 ccagagcagc cgccgcgcct tgtcgaggtt ggcgttgtcg ggccagctgt tcaacggctc
     3121 gaatcgctgc tggccgccgt cggctccacc gcgcccgtcg atggtgcggt aggttccggc
     3181 gctgtgccag gccatgcgga tgaagaaggg gccatagttg ccgtagtcgg cgggccacca
     3241 gtcctgggaa gtcgtcaggg cctgcttgat gtccttcttg accgcctcga ggtcgaggct
     3301 cgagaacgcc ctggcgtagt cgaagtccgc acccagcggg ttcgacctcg cgtcgtgctg
     3361 ccgcagcacg gacaggtcga gttgctcggg ccaccagaac tggttcggca tcggggcacc
     3421 cgaggcgccg agcgcggcgg tcgaagccag tggcagcgcc gcgatcgcgc acgcgacgaa
     3481 cgacgtcatt cgtttccttt gcatcggagt ctcctggtca ggcgagtgat tgaatccgac
     3541 gcaaagatag agagagtctc gctgcgagat gttggattga ttggatcgat acgataggca
     3601 atgtcaatcg aaggggcagg gtaaccggcg ccccccggaa ccgcaggcgt cgatgtgatc
     3661 atcgcgaccc tggaatcagt ccaggggcgg cgcacacgca cccttggggg acatctcagc
     3721 ggtaacttgc cgtgccgccg gcctgcccct agactgcgcc cctgtcgcaa ccccgcgacc
     3781 cggttgaaaa aacgcatcgg agagtcgcgg cgcgtgaggc tgagcccccg gttccttttt
     3841 gccccgatgg cgacgctgct gctcgcggcg gggagcgctt tcgcctccgc ggcggggacc
     3901 gcttgcgacg gagaactggc gcggcagatc ccggcgaggc cgccggaggc gctcaccggg
     3961 ggcgagtttg ccgagcgggc acgcgatctc gccggacctc cgcgcgacgc gatggtgcag
     4021 ggcgaactcg aggcgggcga cgtcccggcc ttcctgcgcc acctggtgcc ggtgacggtc
     4081 agcgacgccc agaacagcgt gaccgtgtgc gtgatgcccg attacctcgc gctgggcacg
     4141 gaccaggatt tcgcctacgt gcccctgggc ctggaggccg cgctcgacgt cgccgaccgc
     4201 ctcggcttca tgctgccgac gcgcaagctc gtgaacgcga tctacgccga atcgaccgtg
     4261 aagctcgatc cccagccgct gcccgccggc gatgccatgc gcagcacgga ctatctcgtg
     4321 cgccacaaca cgatggtgcg cgcgcagcgc cagtcgcgtg gcgccgcact gggagatctc
     4381 actgccggtg acaagaagga cctggtgctc acgccacgcc tgtgggaggt tccgggacgc
     4441 gtcgcgatct acggctggca ccgcgccgtg ggttcgccga tccagccgct cagcaccgtg
     4501 cacggcgccc gctacgccga ctacagccac ggcatccgtc tcgtgagcca ggtcgcctac
     4561 gtgaatggcg tcgagcgccc gctcgtcgac gtgctcgccg acccgcggct cgcatcgctc
     4621 gtgagcgacg agggtccgat gccgcgcctc accgagcgcc tgagtgcgct gcgggccgga
     4681 ctgcgcagcg acatggcggc agcgccgacc gtcgcatggc tgccgcgaca gggcagcagc
     4741 gcgcgggccg ccccctgagc ttcgtccacg gggcgaaact cacttctcgt cgagcgtctt
     4801 gcagcagcga aatcccaggt gatagttggc gtggctgtgc gggtccatga cccggctgcc
     4861 atgccagtac ggcgcgcgcc agcggcacca gtcctcgtgc gcgcggtacg tgtcgaaaat
     4921 gaaccagctg cccttcatct ccgtgacgcc cagcgcggag ctgcccgtgc tcgccatctg
     4981 tgacggatcg agcggcaggt tcatgtgctc ggcggcgttg ccgttcaggt catacacgtc
     5041 gagtggactg cggcaggcag ggaagctccc ggccggatag gtgttcgaac cgcacatgct
     5101 ccagccgccg ccctggcagc ccggcgtctt ggaactcccc gtcgcgcaga tcccgcgctg
     5161 gtactccggg ccgtagctcc agcgcctggt ggccgcgtac tcgcggttgt gcgcggcgcg
     5221 catgcgctgc acggcgatct cggggctcac cccctgcgcc aggtcgaaac ggtagtcggg
     5281 aggcccgagg ctgccctggc aggcgccttc ccactcgtgg gcgtcgcaca gccgcttgcc
     5341 ctcggcagcg cagatctccg ccgcctcgcg cgccttgacc cagatcaccg ggtactcgca
     5401 cgggatgtcc gggaactcga actggtcgat gcaggccctg gcccgcgccg acgtgcccgt
     5461 cgtcgggtcg tagagtggtg ccatgtactt cgcgccgcag atccgctcgt actcggggtt
     5521 ggcatagacg atcccgcgtc gctcgaggtt ctccaggcac tgtgccgggg attccgggtg
     5581 ccggctgatc gccggattgc cctggcccat tgcgaagttg cccgcgaaga tcgcctcgat
     5641 gcgctgcatc tgcgcgggcg tgaggccgtg caccgtcgcc agcttctgaa acagcgcgcg
     5701 gttctgctcg tcgttcgccg tgtcaccccg cgcgcccgcg gccgaggcga ggaccgcgat
     5761 tgcgatgagc gcgaccacgt gtccttgtct catggaggcg cctcgcgtcg cgtcaggtag
     5821 aagaagtcgc ggttgtccgg gaagccgatg aagcgtggcg cgtagttcgt gccgctgccc
     5881 tggtcgagca cgtccatgcc ctggatcagg tgctcgacga gacgttcctt gccgcgagtc
     5941 acgtacagcg cgaggtaggt gtgctccagc gcgttcatgt cgaggtgcag ggcgtactgg
     6001 cagccatacg cctcgaatac gcgcgccatg gcggagggag tcgcgtcgga gaagtagccg
     6061 tagatcagga agcgtcgcgc atcgttcgac tgcaggcaga cgccggcgcg caggctgcgc
     6121 aggtcgccct tgtccgaccc ggaccagttg ccggcgcccc agttcgagac gagggcaccc
     6181 gggacgccgc gacccacggc cgggtcgaac tcgatgaccg gcacgccgtt ctgccgggcg
     6241 tagcggatcc cggacagccg cgaattcccg gccgcgctcc acgtctccag ctcgacgcgg
     6301 ccgtcgtcga aagccagcac cgtggcgagc cccggctgga gcttgctgaa caccacgccc
     6361 tgctcgacga acccgtagtg actgccgtga ttcacctgcg cgagcgctcc gtagcggaac
     6421 gcgccgtggt cgcgcttgaa gccgccggtg aatgtggcga tcgtgcgtgc ggcctccgcc
     6481 gggctcacca tcccgttcac gaccagcggt gccggccggt cgatgccgtc gggccccggg
     6541 agcgcgggat cgcgcacggt gggcagcacg cgatccgacc agccgacgcg cgggtggtcc
     6601 gtgccgagca cgaaatgcag gtcgaaacgc gccaggtcga acgcgaccag gtacacgagc
     6661 gcgtcgagtt cgaccggacc gagcgcattc acgccacggc tgggccccag cagtgagggc
     6721 tccagcgccc ccggcgtcac ggcgctggcg tagacgcccg cgaggtcgcg caccgggtac
     6781 catcggccga ccagcagctc gcgacccggc gcggcgaggt cgagaccaag ttgctccggg
     6841 atgatggcga ggcccgcgcg gtccggcgcg atcgctgccg gccggggcgc ctgcggcatg
     6901 gccgctcgtg tggctgcggc acccgcctcg tgtgtcaccg gcttctcgag gaaggcgtcg
     6961 cggtagacct gctccttgac gaacccgacg agctgctcgc cgccccagac gtggtcgcgc
     7021 aggaaatccg cgactcgctc cagcgacgtg cggcgtccgg cgaccgggtt gcgcagatac
     7081 aggcgcccgt cacacagcgg cgcgtacggc agcccggtgc gactggcctc gtcgagcggc
     7141 gcggcaggtc ggctggtcca cagcgtgcaa ggcgcgccgc gctcgccgac ggcgacgcgc
     7201 agcgaagtag ggtcaccggc gaggagggtg agcgcaatct cccgcggtcg ggggttctcg
     7261 aggtgatagc tcggtccctg cccggtcgcg ccgaactcga gcgtgagcag gtaccaggcg
     7321 ttcgcgtacg ggttcaggtt gaccagcgtc gccgtgcccg cgcgcccatc ggccgcgcgg
     7381 accggcacgg tcgtcgtgct gcggaagggt tgcagttcca ggatcgtctt cggctgctcg
     7441 gctcggtact ccgcggcctg tcgctcgagt tgggcagcgc ccggcagtgc cggtgcgcgc
     7501 aacgtggccg gcgcgcctgc cgtgacggcg gcaccggcgg ccgccagcag cccgaggatc
     7561 acgagtgcgc gcggtgttcg gcccatgatc atgcgccgct acagcgcgag ccacgctgtc
     7621 aggcccatca gcggcaccag gatccagctc agcctgtcct cgaggatgaa gcggagcggg
     7681 tcgtccggca ttctcccgcg gtgcgcgacc agccacaggt ggctgatcca gtacagcagc
     7741 agggtgcagg tgagccagat cacggccgga tggcggtaga gcatgccggc ggtgagcgtg
     7801 ctcgtgtata gcgcgagcac caggaccgcg agatagccgc tcgacacgcc gagcgcgatg
     7861 atcaggctct ggtcctcgac gaggtacgca cgcgcgtggg cgtgcgcacc gtcgatgacc
     7921 tggcgcacgc tgatctcggc gtagcgcttg agcagcgcca ggctgaagaa gaagaagatg
     7981 cagaacgcga gcagccagga cgacgggacg atcgccaccg cgacggcgcc gagcagcacg
     8041 cgcgcggcat agccggtggc gagcaccagc acgtcgagca gcacgatgtc cttgaggccg
     8101 agcgagtagg cgagcatgag cacgaaatac cccgcgagca ccccggccgc gaccaggccg
     8161 agcggccaga cgaccagcag gctcgccgca agcagcagcg gcaccagggt gatcgccagc
     8221 ccgcgcggca ggcgcccggc cgcgatgggg cgccggcgct tgtaggggtg gagccggtcg
     8281 ctggcgaggt cgtgcaggtc gttgaccacg tacaccgcgg aggagcacag gctgaaggcg
     8341 acgaatgcgc gcagtgccgc gtgcagcagc ggcggctcgt ggaggcgatg ggcggctgcg
     8401 agcggcacga agatgagcag gttcttcagc cactggtacg gccgcagcgc atcgagcgcg
     8461 gcgcgcgcca gggacgtgcc ggcgagcagg cgactagaag aagtgtctgg caaggtcgag
     8521 cacgcctctt ttccagcgca cgcgatgcgt cgtgccggtc gccggccgga tctcgttgca
     8581 gtgcgcgacg taccagtcgt aattccgcac gagtgcgtca cggttcgaat accgtggccg
     8641 gtagccgagc ttgcgctcga tgcgctcgat cgacacgaag ctgtcctcgg ccgcggtctc
     8701 gtagatccaa cggtacatcg gcgagagatg cagcgcattg agcgcccgca gcgccaggac
     8761 cgccggcgcc ctgggaagac cgatcgcgtg cccgccgtgc cccgcacgat cgagcacggc
     8821 ctggacattc tcgcgcatcg tgccgaactc cttcgcgccc acgttgaacg tgtcgctcac
     8881 ggcccccggc tcgcgggtca tgcagagctc gatcgcctgg cagagatccg ccacgtccag
     8941 cagctgatag cggttgttgc cggggccgat gagcgggaag ttgcgtcctt cgcgcgccca
     9001 tgcgtacagc aactcgaaca ccccgagtcg ctccggtccg acgaacgtct tggggcggag
     9061 cacgctcacg cagagcccac gggcgcggaa ctcgctgcag agcgcctcgg ccgcgatctt
     9121 ggactcgccg tacggtccga cgccctgcgt gcggtccgtc tcgagcagcg gatggtggtc
     9181 cggaatgccg tacaccgcgg tcgacgagat gaagatgaag cgcacaaccc ggtgcgtgag
     9241 tgcgctctcg agcaggaggc gggtgccatc gacgtccgtc gagcggatct cctctgccgg
     9301 cgcgagcggc agcgcggccg cgcagtgcac gacggtctcc acgtcctgca tggaacgctc
     9361 gacggcggcg ggatcacgga tgtcgccgtc gatcacgttc accgcacttc gctcggggta
     9421 ggcgaagtcg gcgatgtcga tcgagcgcac gcgccggccg atgccgagga ggtggcggca
     9481 gaggttgatg ccgaggaacc cggcaccgcc tgtgaccaac gtgactgggg agggcatccg
     9541 atccggctcc cgggcgcccg catcgggggc gccgctctgc gaccc
//
LOCUS       DAOMFD010000072         9571 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2104_length_9571_cov_33.246742, whole genome shotgun sequence.
ACCESSION   DAOMFD010000072 DAOMFD010000000
VERSION     DAOMFD010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9571)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9571
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2104_length_9571_cov_33.246742"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="PL152_05850"
     CDS             complement(<1..283)
                     /locus_tag="PL152_05850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48837.1"
                     /translation="MTRTHTLLAVLALVFTVALGACSGSKQEASQSAPATAAATGAPA
                     AAQPTAGAATTVAAPGTAGAAAGPLPPPPGIKARSYLLADALSGPELAGL"
     gene            complement(280..1485)
                     /gene="mltB"
                     /locus_tag="PL152_05855"
     CDS             complement(280..1485)
                     /gene="mltB"
                     /locus_tag="PL152_05855"
                     /inference="COORDINATES: protein motif:HMM:NF024798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HQR48838.1"
                     /translation="MSTRRPRQTPSRRQHRIACAALAALAAAPAWALDASREDVAGFV
                     AELSTRYGFDAAALTGLMAQAQSQPSIVASISRPAEKTLSWQEYRARFITDRRVSRGA
                     EVGVAQADALRRAAAGGVPAEYLLAITGVETFYGETTGKYRVVDALATLGFDYPPRSK
                     FFRGQLEQYLLMAREESLDPLVPLGSYAGAMGVPQFMPGSFRAYAVDGDGDGRRDLWN
                     DWSDVFASIANYLLQNGWHPGEPVMTAADVTGANLDGLATDRLALPDTVQSLRDRGVK
                     FETTLPPDAPAVLIPLALADGVEYRVGFSNYLAITRYNRSELYASAVNDLAEDIARAR
                     TAGGAPAPAPRAIIDPSPPSPPPQPPSPTAAVGATEPDTARAGSGSFQSGPAPAPANS
                     PDSTTTPPQ"
     gene            complement(1482..2627)
                     /gene="rodA"
                     /locus_tag="PL152_05860"
     CDS             complement(1482..2627)
                     /gene="rodA"
                     /locus_tag="PL152_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007927107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HQR48839.1"
                     /translation="MKTDIAGPGYSSRARRTLTVTGRLLEALHLDGPLLLAVLGTCVA
                     GLVVLYSAAGEELDVFLAQVARVGLALGVLVAVAQVPPRLMRVAAPWLYALGVLLLVA
                     VAVKGDVSMGAQRWLNLGFVRFQPSELMKLAVPLACAWYLHDRPLPPDLRSIVVLGAA
                     VLVPALLIAEQPDLGTALLVTAGGALVVLLAGLQLRYIVGLGAAVLAAVPVAWHFMHD
                     YQRKRVLMFLNPQDDPLGAGYHTIQSQIAIGSGGLFGKGYMNGSQAQLEFLPERSTDF
                     IFAVIGEEWGLVGLGVLMALYMFVIARGLFIAVQAQDTFSRLMAGALTLTFAVYVFVN
                     SGMVAGLLPVVGVPLPLVSYGGTSMVTLMAGFGILMSIHSRRKLVST"
     gene            complement(2624..4567)
                     /gene="mrdA"
                     /locus_tag="PL152_05865"
     CDS             complement(2624..4567)
                     /gene="mrdA"
                     /locus_tag="PL152_05865"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR03423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HQR48840.1"
                     /translation="MARTVRLKDHWAEQRMFGRRVFAATVVMLVCLAALGARLAYLQI
                     LRHEYFSELSQGNRIRIDPVPPPRGLILDRHGVPLALNRPAYQLEITHEQTPNLEDTL
                     RRLVKLGLLPAEDYDRVLHMIKARRSFESVPVRLQLSEEELARFAVNRQDFPGVEVRP
                     RLTRYYPLAGTGVHALGYVAAISEADEKRIDPANYAGTTLIGKLGVERAYEDELHGET
                     GYQQLLVNAQGRHVERVGTQAAELERRAPVAGNDLYLSVDARVQQAAEQALAGQRAAV
                     VAIDPHNGDILAFVSTPTFDPNGFARGLSYAEYAALAENIDVPLYDRALRGVYPPGST
                     VKPLVALAALEDGVIDPDATRFCQGVFQLPGDSHRFRDWKKGGHGTVNMHSAIAQSCD
                     VYFYGVAERLGIQRLHDFLAQFGLGEKTGIDIEGERSGLLPSPAWKKKAFKRKDLQVW
                     FPGETVIAGIGQGYMLVTPLQLAHAAATISMRGKRYAPRLLTRVRDASTGAIRDLPPR
                     ELPPVKVSDPTFWDVIIGGMVGVTNDWNGTAHRVQAGAPYKIAGKSGTAQVFGLKQNE
                     KYNEKDVAERLRDHGLFVAFAPADDPKIAVAVVVENGRSGSGTAGPIARKVIDAYLMP
                     DSVTDGETGSQPAPPAPQAGSDE"
     gene            complement(4583..5065)
                     /gene="mreD"
                     /locus_tag="PL152_05870"
     CDS             complement(4583..5065)
                     /gene="mreD"
                     /locus_tag="PL152_05870"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HQR48841.1"
                     /translation="MRRSMFGRRILYWFSVAVGLALAIVPLPGWLDAARPDLALLAVV
                     YWILASPRIAGLWYAWLAGLFLDVLRGMVLGQHALAFLVIAFLTHRLQLRMRMFPIVQ
                     QAGVIMGLLAVYHFILFWTDGLTGHGYTGWNRWLPVLTGALLWPLIVAAGDTLTRRSR
                     "
     gene            complement(5062..6273)
                     /gene="mreC"
                     /locus_tag="PL152_05875"
     CDS             complement(5062..6273)
                     /gene="mreC"
                     /locus_tag="PL152_05875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HQR48842.1"
                     /translation="MVELSHDSRPIIGRGPPLAATFLALATASVALMVADVRYDQLGR
                     VRGWLDAAAYPVERAIDLPFRAWDWLTGSFADRDRLRRENLELTGSLRLANLQLQRFE
                     ALEAENLRLREMRANSAAVAKRAMVASILNVDLDPFRHRVLLDKGAADGVFKGQAVLD
                     AQGIFGQVWRVNARTSEVILISDAEHAIPVQSNRSGVRTIAVGTGDPNVLSLPFVTVE
                     SDLKKDDLLITTGMGGVFPPGYPVARVTKVERTAKATFAIVEARPTATLDRDREVLLV
                     WFDAPAPGAPVRLAPTAAVPEAKAPPAAPAAQPNTPAPSATGAAPAPQPGAAPASPPG
                     TAPSEAPRPAAEAHAPPPTGTTGEPPATEATPAGPSQAPEAGPGAEPATPAETPAPQP
                     EPATTPPGGTR"
     gene            complement(6302..7345)
                     /locus_tag="PL152_05880"
     CDS             complement(6302..7345)
                     /locus_tag="PL152_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HQR48843.1"
                     /translation="MFKKLRGYFSNDLSIDLGTANTLIYVRGKGIVLNEPSVVAIREE
                     PSRGGKKIEAVGTEAKNMLGRTPGNITAIRPLKDGVIADFTVTEKMLQYFIEKVHGNR
                     LIRPSPRVLVCVPYGSTQVERRAIQESAEGAGARWVRLIEEPMAAAVGAGMPVHEARG
                     SMVLDIGGGTSEVAVISLNGIVYAASVRIGGDRFDEAIINYVRRNYGILIGEATAERI
                     KIEIGSAYPGQHVKEISVKGRNLSEGVPRSFTLNSNEILEALQEPLQGIVGAVKAALE
                     QTPPELGSDVAERGIVLTGGGALLRDIDKLLMEETGLPVVVAEDPLTCVARGGGKMLE
                     LIDEHGPGLFGLD"
     gene            7482..7772
                     /gene="gatC"
                     /locus_tag="PL152_05885"
     CDS             7482..7772
                     /gene="gatC"
                     /locus_tag="PL152_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004574467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HQR48844.1"
                     /translation="MSLTRAQVEGIAHLARLEIVEARMPVYVDSLSRIIEFVEQLSAA
                     DTAGVEPMAHPLADQVQRLRPDAVTEEDRHEKYQQNAPAVAAGLYLVPKVIE"
     gene            7826..9292
                     /gene="gatA"
                     /locus_tag="PL152_05890"
     CDS             7826..9292
                     /gene="gatA"
                     /locus_tag="PL152_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HQR48845.1"
                     /translation="MHSLSVTELAAGLRARKFSSLDLTLHFLGRIERLGPQLNAFVTV
                     TADRALADARAADEVLARGEGGPLTGVPIAHKDIFCTDGVLTTCSSRMLSNFVAPYDA
                     TVVERWKAAGTVMLGKTNMDEFAMGSSNETSYFGPVRNPWDPSRVPGGSSGGSAAAVA
                     ACLAAAATGTDTGGSIRQPAALTNLTGLKPTYGRVSRYGMIAFASSLDQAGVLTRSAE
                     DAALLLGAMAGFDPRDSTSVDRPVPDYVAELAQPLAGVRIGIIREFFGAGLDPAVGKL
                     VQDAIGALERAGARVREVSLPALPLSVPTYYVVAPAECSSNLSRFDGVRFGHRCENPR
                     DLLDLYKRSRGEGFGDEVKRRIMTGTYVLSAGYYDAYYLKAQKVRQLIAADFARAFGE
                     VDVVLGPTAPTAAFELGAKTSDPITMYLNDIYTIGANLAGLPAISIPCGFVTESGRDL
                     PVGLQLVGPHFAEARLLGISHQYQRLTDWHRRLPAEYA"
     gene            9292..>9571
                     /gene="gatB"
                     /locus_tag="PL152_05895"
     CDS             9292..>9571
                     /gene="gatB"
                     /locus_tag="PL152_05895"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185791.1"
                     /note="allows the formation of correctly charged
                     Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation
                     of misacylated Asp-tRNA(Asn) or Glu-tRNA(Gln) in organisms
                     which lack either or both of asparaginyl-tRNA or
                     glutaminyl-tRNA synthetases; reaction takes place in the
                     presence of glutamine and ATP through an activated
                     phospho-Asp-tRNA(Asn) or phospho-Glu-tRNA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HQR48846.1"
                     /translation="MEWETVIGLEIHAQLATRSKIFSGSSTAFGAGPNTQANLVDLGY
                     PGVLPVLNHEAVRMAVCFGLATGSTIARRSVFARKNYFYPDLPKGYQIS"
BASE COUNT         1408 a         3306 c         3416 g         1441 t
ORIGIN      
        1 gcaggccggc cagctcgggg ccgctcagtg cgtcggccag gaggtaactg cgcgctttga
       61 ttccgggcgg cggcgggagc gggcccgccg ccgcgccggc ggtgccgggt gcggcgacgg
      121 tcgtggcggc acccgccgtc ggctgggcgg ctgcgggggc accggttgcc gcggcggtgg
      181 ccggggcgga ctgcgaggct tcctgcttcg agccagagca cgcgcccaag gctacggtga
      241 agacgagggc gaggactgcg aggagcgtgt gtgtgcgggt cattgcgggg gggtggtggt
      301 tgaatccggt gagttggcag gtgcgggcgc cggcccggac tggaacgagc cgctgcccgc
      361 gcgggcagtg tcgggttccg ttgcacccac ggcagccgtc ggcgacggcg gctgcggagg
      421 cggcgatggc gggctggggt caatgattgc ccgaggtgcc ggcgcaggcg cacctccggc
      481 ggtgcgcgcc ctggcgatgt cctcggcgag gtcgtttacc gcgctcgcat acagctcgct
      541 gcggttgtag cgcgtgatcg cgaggtaatt gctgaagccg acccggtact cgacgccgtc
      601 ggcgagggcg agcgggatca gcaccgcagg cgcgtcggga ggcagcgtgg tctcgaactt
      661 cactccccgg tcgcgcagcg actgcaccgt gtccggcaat gcgagccggt cggtggcgag
      721 gccatcgaga ttcgccccgg tgacgtcggc cgccgtcatc accggttcgc cgggatgcca
      781 gccgttctgc agcaggtagt tcgcgatgct cgcgaacacg tcgctccagt cgttccacag
      841 gtcgcggcgg ccgtcgccgt cgccgtccac ggcatacgcg cggaagctgc cgggcatgaa
      901 ctgcggcacg cccatcgcgc cggcatagga gccgagcggg accagcgggt cgagggattc
      961 ctcgcgcgcc atgagcaggt actgctcgag ttgcccgcgg aagaacttgc tgcgcggcgg
     1021 gtaatcgaag ccgagcgtgg cgagcgcgtc gaccacccgg tacttgccgg tggtctcgcc
     1081 gtagaaggtc tcgacgcccg tgatcgcgag caggtactcg gccggcacgc cgcccgccgc
     1141 ggcacgtcgc agggcgtccg cctgcgcgac gccgacctcg gccccgcgcg acacgcgccg
     1201 gtccgtgatg aagcgagcgc ggtactcctg ccacgacagg gtcttctccg ccggcctgct
     1261 gattgacgcg acgatcgacg gttgcgactg cgcctgcgcc atcaggcccg tgagcgccgc
     1321 tgcatcgaag ccgtatcgcg tgctcaactc ggcgacgaag ccggcgacgt cctcgcggct
     1381 tgcgtcgagc gcccacgccg gtgccgctgc aagcgcggcg agggccgcgc aggcaatgcg
     1441 atgctgccgt cgcgacggcg tttgccgcgg gcgtcgtgtg ctcacgtcga gaccagtttc
     1501 cggcgggagt ggatggacat caggataccg aagccggcca tgagggtcac catcgacgtg
     1561 ccgccgtagc tcacgagcgg cagcggaacg ccgacgacgg gcagcagccc ggcgaccatg
     1621 ccgctgttga cgaacacgta cacggcgaag gtgagcgtga gcgcgcccgc catcaatcgc
     1681 gagaaggtgt cctgtgcctg gaccgcgatg aacagtccgc gggcgatcac gaacatgtag
     1741 agcgccatca gcacgccgag accgacgagc ccccattcct cgccgatcac cgcgaagatg
     1801 aagtccgtcg accgctccgg caggaattcg agctgcgcct ggctgccgtt catgtagccc
     1861 ttgccgaaca gcccgccgga cccgatcgcg atctgcgact ggatcgtgtg atagccggcg
     1921 ccgagcgggt cgtcctgcgg gttgaggaac atgaggacgc gcttgcgctg atagtcgtgc
     1981 atgaagtgcc aggccacggg caccgccgcc agcaccgcgg cacccagccc gacgatgtag
     2041 cgcagctgca agccggccag cagcacgacc agcgcgccac ctgccgtgac gagcagcgcc
     2101 gtgccgaggt cgggctgctc tgcgatcagc aatgccggca cgagcacggc ggcaccgagc
     2161 accacgatcg agcgcaggtc gggtggcagc gggcggtcgt gcaggtacca ggcgcaggcg
     2221 agcggcacgg cgagcttcat cagttccgac ggctggaacc gcacgaatcc caggttcagc
     2281 cagcgctggg cgcccatgga cacgtcgccc ttgaccgcga ccgcgacgag cagcagcacg
     2341 ccgagcgcat acagccatgg cgcggcgacc cgcatcaggc gcggggggac ctgtgccacc
     2401 gcgaccagca cgccgagcgc gaggccgacg cgcgccacct gcgcgaggaa cacgtcgagt
     2461 tcctcgcccg cggcgctgta gaggacgacg agtcccgcca cgcaggtccc gagcaccgcc
     2521 agcagcagcg gcccgtcgag atgcagcgcc tcgagcagcc tccccgtgac ggtcaacgta
     2581 cggcgggcgc ggctggagta ccccgggccg gcgatgtcgg tcttcattcg tcggatcctg
     2641 cctgaggtgc cggcggtgcg ggctgcgatc ccgtctcacc gtcggtgacg gagtctggca
     2701 tcaggtaggc gtcgatcacc ttgcgcgcga tcgggcccgc ggtgccgctg ccgctgcggc
     2761 cattctcgac gaccacggcc acggcgatct tcggatcgtc ggccggcgca aatgcgacga
     2821 acaggccgtg atcgcgcagt cgctcggcga cgtccttctc gttgtacttc tcgttctgct
     2881 tgaggccgaa cacctgtgcc gtgccggact tgccggcgat cttgtacggc gcacccgcct
     2941 gcacgcgatg cgccgtcccg ttccagtcgt tggtcacgcc gaccatgccg ccgatgatca
     3001 cgtcccagaa cgtcgggtcg ctcaccttga ccggcggcag ctcgcgcggc ggcaggtcgc
     3061 ggatcgcgcc ggtgctcgca tcccgcacgc gggtcaggag acgcggcgca tagcgcttgc
     3121 ctcgcatgga gatcgtggcc gcggcatgcg cgagctgcag cggcgtgacc agcatgtatc
     3181 cctggccgat gccggcgatg acggtctcgc cggggaacca gacctgcagg tccttgcgct
     3241 tgaacgcctt cttcttccac gcgggcgagg gcagcaggcc gctgcgctcg ccctcgatgt
     3301 cgatgccggt cttctcgccg agaccgaact gcgccaggaa gtcgtgcagg cgctggatgc
     3361 cgaggcgctc ggcgacgccg tagaaataga cgtcgcacga ctgtgcgatc gccgaatgca
     3421 tgttgacggt gccgtggccg cctttcttcc agtcgcgaaa ccggtggctg tcgccgggca
     3481 gctggaacac gccctggcag aatcgcgtcg catccgggtc gatcacgccg tcctcgagcg
     3541 cggcgagcgc gaccaggggc ttcacggtcg agcccggcgg gtacacgccg cgcagcgcgc
     3601 ggtcgtagag cggcacgtcg atgttctccg cgagcgcggc atactcggca taggacaggc
     3661 cgcgcgcgaa tccgttcgga tcgaacgtcg gcgtgctcac gaaggcgagt atgtcgccgt
     3721 tgtgcgggtc gatcgccacg acggcggcgc gctggcccgc gagcgcctgc tcggcggcct
     3781 gctgcacgcg cgcatccacg gaaagataca ggtcgttgcc cgccacgggt gcgcgccgct
     3841 cgagttccgc ggcctgcgtt cccacgcgct caacgtggcg gccctgcgcg ttcacgagca
     3901 gctgctggta gccggtttcg ccgtgcagct cgtcctcgta agcgcgctcg acgccgagct
     3961 tgccgatcag cgtcgtgccc gcgtagttcg ccgggtcgat gcgcttttcg tccgcctcgc
     4021 tgattgctgc cacgtacccc agcgcatgca ctccggtgcc ggcaagcggg tagtagcgcg
     4081 tcagccgcgg acgcacctcg accccgggga agtcctggcg gttcacggcg aagcgcgcaa
     4141 gctcctcctc ggagagctgc agccggaccg gtacggattc gaagctgcgc cgcgccttga
     4201 tcatgtgcag cacgcggtcg tagtcctccg ccggcagcag tccgagcttg accagccgcc
     4261 gcagggtgtc ctcgaggttc ggggtctgct cgtgcgtgat ttcgagctgg taggccggcc
     4321 ggttcagggc gagcggcacg ccgtggcggt cgaggatcag tccccggggc ggcggcacgg
     4381 gatcgatgcg gatccggttg ccctgggaga gttcgctgaa atactcgtgg cgcaggatct
     4441 gcaggtaggc aagccgtgcc ccgagcgccg ccaggcagac gagcatgacc acggtggccg
     4501 cgaatacgcg gcggccgaac atgcgctgtt cggcccagtg gtccttgagt cggacggtgc
     4561 gcgccatcgt gcggcaggcg gctcagcgcg agcgccgcgt cagcgtgtcg ccggccgcga
     4621 cgatcagcgg ccagagcagc gcgccggtga gcaccggcag ccagcggttc cagcccgtgt
     4681 agccgtggcc ggtcaggccg tcggtccaga acaggatgaa gtggtagacg gcgagcagtc
     4741 ccatgatcac tccggcctgc tgcacgatcg ggaacatgcg catgcgcagt tgcaggcggt
     4801 gcgtcaggaa cgcgatcacg aggaaagcga gtgcgtgctg ccccagcacc atgccgcgca
     4861 gcacgtcgag gaacagcccg gccagccacg cgtaccagag cccggcgatc cgggggctcg
     4921 ccaggatcca gtacacgacg gcgagcagcg cgaggtccgg gcgcgccgcg tcgagccagc
     4981 cgggcaaggg cacgatggcc agggcgagcc ccacggccac gctgaaccag tacaggatcc
     5041 ggcgcccgaa catgctgcgc ctcatcgcgt gcctcccggt ggcgtcgtcg caggctcggg
     5101 ctgcggcgca ggggtctcgg cgggtgttgc gggctcggcg cccggacccg cctctggtgc
     5161 ctgcgacggc ccggcgggtg tggcctcggt cgcgggcggc tctccggttg tcccagtcgg
     5221 tggcggagcg tgcgcttccg ctgcggggcg cggcgcttcc gatggagcgg tgcccggagg
     5281 gctcgcagga gcagcgcccg gctgcggagc gggcgcggca cccgtcgcgc tgggtgcggg
     5341 agtgttcggt tgcgcggccg gcgcggcggg cggtgccttg gcctcgggca ccgcggccgt
     5401 gggagccagg cgtacgggcg cacccggtgc cggcgcgtcg aaccacacga gcaggacctc
     5461 gcgatcacgg tcgagcgttg ccgtcggcct cgcctccacg atcgcgaacg tggccttggc
     5521 ggtgcgctcg actttcgtga cgcgcgcgac cgggtacccg ggcggaaaca cgccgcccat
     5581 gccggtggtg atgagcaggt cgtctttctt gaggtcggac tcgacggtca cgaacgggag
     5641 cgaaagcacg ttcgggtcgc cggtgcccac cgcgatcgtg cgcacgccgc tgcggttcga
     5701 ctgcaccggg atcgcgtgct ccgcgtcgct gatcaggatc acctcggagg tgcgggcgtt
     5761 cacgcgccag acctggccga agatgccctg cgcgtcgagc accgcctgtc ccttgaacac
     5821 gccgtcggcg gcgcccttgt cgagcagcac ccggtggcgg aacgggtcga ggtccacatt
     5881 gaggatcgag gccaccatcg cgcgcttggc gacggccgcg gagttggcgc gcatctcgcg
     5941 cagccgcagg ttctcggcct cgagggcctc gaagcgctgc aactgcaggt tggcgagcct
     6001 gaggctcccg gtgagttcga ggttctcgcg gcgcagccga tcgcgatccg cgaacgaccc
     6061 ggtcagccag tcccaggcgc gaaacgggag gtcgatcgcg cgctcgaccg gataggccgc
     6121 tgcatcgagc cagccgcgga cgcgcccgag ctggtcgtag cgcacgtcgg cgaccatgag
     6181 cgcgacggac gcggtcgcca gcgcgaggaa cgtggccgca agcggcgggc cgcgaccgat
     6241 gatcggccgg gagtcgtggc tcagttcgac cacgggacca gcagtgcggt cacgcccgca
     6301 ctcaatccag gccgaacagg cccgggccgt gctcgtcgat cagctccagc atcttgccgc
     6361 cgccgcgcgc cacgcaggtg agcgggtctt ccgccaccac gaccggcagg cccgtctctt
     6421 ccatgagcag cttgtcgatg tcgcgcagca gcgcgccacc gcccgtgagc acgatgccgc
     6481 gctcggcgac gtccgagccg agttccggcg gcgtctgctc gagcgcggcc ttcaccgcgc
     6541 cgacgattcc ctgcagcggc tcctgcagcg cctcgaggat ctcgttggag ttgagcgtga
     6601 agctgcgcgg cacgccctcg gacaggttgc ggcccttcac cgagatttcc ttgacgtgct
     6661 ggccggggta ggccgagccg atctcgatct tgatgcgctc ggcggtcgct tcgccgatca
     6721 ggatgccgta gttgcggcgc acgtaattga tgatggcctc gtcgaagcgg tcgccgccga
     6781 tgcggaccga cgccgcgtac acgatgccgt tcagcgagat caccgcgact tccgacgtgc
     6841 cgccgccgat gtcgagcacc atggacccgc gcgcctcgtg cacgggcatc ccggcgccca
     6901 cggcggccgc catcggctct tcgatcagtc ggacccagcg cgcgcccgcg ccttcggcgg
     6961 actcctggat cgcccggcgc tcgacctgcg tcgagccgta aggcacgcac accagcacgc
     7021 gcgggctggg gcggatgagg cgattgccgt gcaccttctc gatgaagtac tgcagcatct
     7081 tctcggtgac ggtgaagtcc gcgatcacgc cgtccttcaa cggccggatc gcggtgatgt
     7141 tgccgggcgt gcggcccagc atgttcttcg cctcggtgcc cacggcctcg atcttcttgc
     7201 cgccgcgcga tggttcctcg cggatcgcga ccacggaggg ctcgttcagc acgatgccct
     7261 tgccgcgcac gtagatcagg gtgttggcgg tgccgaggtc gatcgagaga tcgttggaaa
     7321 aatagccgcg aagtttcttg aacatctagg agggagtgaa gccggagagc agggccgcgc
     7381 gggctcgcgc ggaattcgcg caattctgac catactcagg acattcggca aggcgacgtg
     7441 tatgatttgc cgcgcatttt tccctcttcc cgagcccctc catgagcttg acacgcgccc
     7501 aggtcgaggg catcgcccac ctcgcccgcc tcgagatcgt cgaggcccgg atgccggtat
     7561 acgtcgacag cctgtcccgc atcatcgaat tcgtcgagca gctgtcggct gccgacacgg
     7621 cgggcgtgga gcccatggcc cacccgctcg ccgaccaggt ccagcgactg cgcccggacg
     7681 cggtcaccga ggaggaccgg cacgagaagt accagcagaa cgcaccggcg gtcgcggccg
     7741 gcctgtacct ggtacccaag gtcatcgagt agcaggctgt ccgccggccc cggtcacagt
     7801 cgcaaggtcc actttggatt cccgcatgca ctccctctcc gtcaccgagc ttgccgccgg
     7861 cctgcgcgcc cggaagttct ccagcctcga cctgacgctg cacttcctgg gccggatcga
     7921 gcggctcggc ccgcagctga acgcgttcgt gaccgtcacg gcggaccgcg cgctcgccga
     7981 cgcacgggcc gcggacgagg tgctggcgcg cggcgagggc gggccgctca ccggcgtgcc
     8041 catcgcgcac aaggacatct tctgcaccga cggcgtgctg acgacctgct cgtcgaggat
     8101 gctgtcgaac ttcgtcgcgc cgtacgacgc gaccgtggtc gagcgctgga aggccgccgg
     8161 cacggtgatg ctcggcaaga ccaacatgga cgagttcgcg atgggctcgt ccaacgagac
     8221 gagctacttc ggcccggtgc gcaatccctg ggacccgtcg cgcgtgccgg gcggctcgtc
     8281 cggcggctcc gcggcggcgg tcgcggcctg tctcgccgcc gcagcgaccg gcacggacac
     8341 cggcggctcg atccgccagc ccgcggcgct cacgaacctg acgggcttga agccgacgta
     8401 cggccgcgtg tcgcgctacg ggatgatcgc gttcgcctcg agtctcgacc aggcgggcgt
     8461 gctcacgcgc tcggccgagg acgcggccct gctgctcggg gccatggcgg gtttcgatcc
     8521 gcgcgactcc accagcgtgg accggcccgt gccggattac gtggcggagc tggcgcagcc
     8581 gctggcaggc gttcgcatcg ggatcatccg cgaattcttc ggcgcagggc tcgatcccgc
     8641 ggtaggcaag ctcgtgcagg acgcgatcgg ggcgctcgag cgcgccggcg ccagggtgcg
     8701 cgaggtgagc ctgccggcgc tgccgctgtc ggtgccgacg tactacgtcg tggcgcccgc
     8761 ggagtgttcc tcgaacctgt cgcgcttcga cggcgtgcgc ttcggccacc gctgcgagaa
     8821 cccgcgcgac ctgctcgacc tgtacaagcg ctcccgcggc gagggattcg gcgacgaggt
     8881 caagcgccgg atcatgaccg ggacctacgt gctttcggcc ggctactacg acgcctacta
     8941 cctcaaggca cagaaggtgc gccagctgat cgccgcggat ttcgcgcggg cgttcggcga
     9001 ggtggacgtc gtcctcgggc ccacggcgcc cactgcggcc ttcgaactcg gcgcgaagac
     9061 cagcgacccg atcacgatgt acctgaacga catctacacg atcggtgcga atctcgccgg
     9121 gctgccggca atctccattc cctgcgggtt cgtgacggag tctggccgcg acctcccggt
     9181 cggcctgcag ctcgtcgggc cgcatttcgc ggaggcgcgc ctgctcggta tctcgcacca
     9241 gtaccagcgc ctcaccgatt ggcatcggcg gctgccggcg gagtacgcgt gatggaatgg
     9301 gagaccgtca tcggcctgga gatccacgcg cagctcgcga cccggagcaa gatcttctcc
     9361 ggttcgtcca cggcattcgg cgccggaccg aacacgcagg ccaacctcgt ggacctcggg
     9421 taccccggtg tgctgccggt cctgaaccac gaggccgtgc gcatggcggt gtgcttcggg
     9481 ctcgccacgg ggtcgaccat cgcgcgccgc tcggtgttcg cgcgcaagaa ctacttctac
     9541 ccggacctgc cgaagggcta ccagatcagc c
//
LOCUS       DAOMFD010000073         9418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2172_length_9418_cov_34.636441, whole genome shotgun sequence.
ACCESSION   DAOMFD010000073 DAOMFD010000000
VERSION     DAOMFD010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2172_length_9418_cov_34.636441"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5340)
                     /locus_tag="PL152_05900"
     CDS             complement(<1..5340)
                     /locus_tag="PL152_05900"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HQR48847.1"
                     /translation="MNAMLAIAPDETHASLQLVVREVAAELNEARAALEAYAENAGDR
                     AALHRFGEHLHVARGALRMAEVYGGALLGEEMEQVVDYLESHERGGQVDADGLDALMR
                     AMEQLPAYVDRVASGGRDVPLALLPLLNDLRAVRGGALLSEGTLLMLNLRSDEPARPG
                     GDVQVPAAELARKLRPRLQLALLGWIRNEQVAENLGALSDIAGQFEQAATTQPLFQLW
                     WVVAALIESLQQGGIDGNVSVKRLLGQVDRELRRLQEQGEARYSATPPVELLNNLLFY
                     VARSFSSGARVLAVRRSFRLDEVLGVDEQLSEAQATLSAPSVRLMKTVAAAIREDLTR
                     VKDVLDIFVRRGATQVDELAPQLEMLRKIGDTLGVLGLGALRDRVQQKLESLAHIVER
                     REPPGDAALLDIASALIEVEDSLDAQLVKLIVSDGGTPEGKAAGAVEPGEPTDEEFKQ
                     VQDAVLRECIVNMARIKEAVTHALAAPSEAQGLDQVPQLVRGITAGLLMLGKQRAVEV
                     MESVGRALGAIVRPDAAALAPQRLDSLADAIVAVEYYMEMLQAGRPDPWHMLENAERC
                     LVDLEEAPTKVVALRPARPQEEPVAAAPPVTPPQQQQPEAAASPPPVPPLAPAAVAAP
                     APAEEAATAVDPEFRQLFVEEARENILVMCELFPQWESNPLDVAALRDLRRVFHTLKG
                     SGRMVGARRVSEFSWSIEALLNRVISQTLPRSPEIVAVVRDAVAAMPKLVDELEHGRA
                     AQVDAAAIVTRADTLAGRETAAMPPAAAAPAKVPEPAAMDPVLRDIFRKETAGHVEAV
                     REYIARCAEAVAPYAVTEALYRACHTLSGIAKTAGARQGIKVAEPMEDYVRKLHDNGR
                     GLPEEGLLLLRDTVQSLETVVDHVEEDTGYFPDQGRLIAGWHALDRALDAELARQAEA
                     GEAAAAESWAEEPARLAEAVPQEAPAAVAAEREIAEPRPLMPELPAEMPAPAAARFEV
                     PSAPVAPEPAAEIEPDFDADIAAIFGEESSELLEQADSSLASWTADRADRSRITELKR
                     VLHTLKGGARMAGLRAMGDLSHEIESFLASLEAGSIASGEPAIAVLQSALDELHRMRE
                     AANAGQRIAPARSLIERVRAVASASAAPAAAPETPAVVGPPAAAAATEAPAVAPLVVE
                     RAAPPMPTEQEGIAAHAEILGPEAEEPPVVAYPAEAAEAAEVVEPATAVGAPPAPALG
                     EHVPIVAEAETRELPPAGVPFVLPGREPAPPPERHELARVSADLLDELLNNAGEVSIF
                     RARVEQQMTSTEFHLAELGRTVTRLREQLRKLELETEAQILFKHQGDTHGRADFDPLE
                     LDRYSTIQQLSRALAESVSDVASIESLLESVNKDGQNLLQQQGRIITELQNGLMRTRM
                     VPFQRHAQRLTRLVRQAAQEAGKKAELTIEGGSGEIDRQVLERMLPPFEHMLRNAVVH
                     GIELPENRLARGKAETGQIVVRLQREGAEVVITVEDDGGGLDIGAIRDKARHMGLLRP
                     EQSLTDEEALQLILEPGFSTAQRLTQQAGRGVGMDVVATEVKRLGGGLFIESWAGRGA
                     RFTIRLPFTLAITQALVVRVHDEIYALPVATVEGVARIPASEVRAHLAEDQPTFEYGG
                     HRYRFQHLGGFLGSGPSVLPDSEAAVPVILVRAGEHSTALVTDELVGSREIVVKSVGP
                     QVASVRGISGATILGDGRIVIILDMGALVRSEWRTRAADASVRPTRDDRIFALVVDDS
                     ITVRRVTQRLLERNGMRVMTAKDGVEAMALLQEHTPDVILLDIEMPRMDGYEVA"
     gene            complement(5360..7432)
                     /locus_tag="PL152_05905"
     CDS             complement(5360..7432)
                     /locus_tag="PL152_05905"
                     /inference="COORDINATES: protein motif:HMM:NF012245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HQR48848.1"
                     /translation="MSNRFDAARLQPTLIRVALLAAVLAGLVFALQWRLTGGQSRVGE
                     QVTALSALAQGMPLQASAAVRGSAAAFDSLAASRARLDAAVKAAGTSEAGLPGDRATW
                     LAMLEQSQAVLDGRAAALEAQKAAAGVRELTPQVVAATANVAKVMGAARPDAFNRQLD
                     RFELRAQAIDQDFSALADGTASVEAAGRRLAESLEYMGQVVGGLVGESNPLGITAAQG
                     PAADAAHLLAAVFRSEQQQVRAVIALGDSLEQMQAAGNALAESGAKASASLARLQAPA
                     PSGARAWLASPWVPLGLLGLAVAALALLVFLQRSIATVQRASEHQAVQSERNQQAILR
                     LLDELSSLADGDLTVQATVTEDITGAIADSINYAIEALRELVTTINDSAIKLDAATRQ
                     TQALSTHLAKASSAQSKQIASASESIAAMASSTEEVSGNAERSADVARHSVDIAHKGG
                     DAVRRTIDGMNAIRENIQETSKRIKRLGESSQEIGNIVELINDIAEQTNILALNASIQ
                     ASMAGEAGRGFAVVADEVQRLAERAANATKQIEVLVRTIQADTNEAVVSMERTTTDVV
                     GGALLAENAGAALEEIEQVSNQIASLVQNISASARQQAASSQNISRNMQVLREISSQT
                     AESTTATSDSIGKLAELAAQLRKSVTGFRLPERPGASVSGEYPVLKPEALAADEVERL
                     RRVGGRAG"
     gene            complement(7472..8038)
                     /locus_tag="PL152_05910"
     CDS             complement(7472..8038)
                     /locus_tag="PL152_05910"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQR48849.1"
                     /translation="MEDARALRSLRDRPFELLRELDRRARAAAQGQPEAAASGAEWVG
                     IAFRLGGEAFLLAREETREVMGFPAAVTRVPGAKSWIRGLSNVRGQLMPVIDVRAFLG
                     SGATSVTRATRVLVANHREIPAGLVVDEVMGFRRFYEGEFSSDLPPTLLRCERYLAGA
                     FKRGAEVWPVFSVRGLLESQQFLQAAAS"
     gene            complement(8040..8405)
                     /locus_tag="PL152_05915"
     CDS             complement(8040..8405)
                     /locus_tag="PL152_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020295795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR48850.1"
                     /translation="MALVLIVDDSPTDVHVMQKALEEGGFRTASAADGGEGIRKAREL
                     HPDLILMDIVMPGLNGFQATRELANDPLTRAIPVIMVTSKSQESDRVWGLRQGAVDYL
                     VKPVAMDQLVRKAQAALAT"
     gene            complement(8432..8839)
                     /locus_tag="PL152_05920"
     CDS             complement(8432..8839)
                     /locus_tag="PL152_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004901860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR48851.1"
                     /translation="MENTAASASRPRLAGLKVLVIDDSKTIRRTAETLLAREGCEVFT
                     AVDGFDALSKVADHQPDIVFVDIMMPRLDGYQTCSLIKHNKVFRTIPVIMLSSKDGLF
                     DRARGRIVGSEHYLTKPFTREELLSAIETHVRR"
     gene            9012..>9418
                     /locus_tag="PL152_05925"
     CDS             9012..>9418
                     /locus_tag="PL152_05925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQR48852.1"
                     /translation="MNGKPLRVAVVMDPIEDIKPAKDTTLAMMLAAQRRGWELHYLEL
                     GHLWLRDGVALGRAHPVTVRDDASDWYSRGDAAVRPLGEFDAILMRKDPPFDTEYIYA
                     TYILERAESAGALVVNRPQGLRDMNEKVYTAWFP"
BASE COUNT         1254 a         3447 c         3287 g         1430 t
ORIGIN      
        1 ggcgacctcg tagccgtcca tgcgcggcat ctcgatgtcg agcaggatca cgtccggcgt
       61 gtgctcctgc agcagcgcca tggcctcgac cccgtccttg gccgtcatga cacgcatgcc
      121 gttgcgctcg agcaggcgct gcgtgacgcg tcgcaccgtg atcgaatcgt ccaccaccag
      181 cgcgaagatg cggtcgtcgc gggtcgggcg caccgaggca tccgccgcgc gggtgcgcca
      241 ctcggagcgc accagcgccc ccatgtcgag gatgatcacg atgcgcccgt cgccgaggat
      301 ggtggccccg gagatgccgc gcacggaggc gacctgcggc cccaccgact tcaccacgat
      361 ctcgcggctg ccgaccagtt cgtcggtcac cagcgcggtc gagtgctcgc ccgcgcgcac
      421 caggatcacc ggcaccgccg cctcgctgtc cggcagcacg gacggcccgc tgccgaggaa
      481 gccgccgagg tgctggaagc ggtaccggtg gccgccgtat tcgaacgtcg gctggtcttc
      541 ggcgaggtgg gcgcgcacct cggaggccgg gatgcgcgcg acgccctcga ccgtcgcgac
      601 cggcagcgcg tagatctcgt cgtgcacgcg cacgacgagc gcctgcgtga tcgccagcgt
      661 gaacggcagg cggatggtga agcgcgcgcc gcggccggcc caggactcga tgaacagccc
      721 gccgccgagc cgcttgacct cggtggcgac cacgtccatg ccgacgccgc gtcccgcctg
      781 ctgcgtgagg cgctgggcgg tcgagaagcc gggctcgagg atgagctgca gcgcctcctc
      841 gtccgtcagg ctctgctccg gccgcagcaa tcccatgtgc cgcgccttgt cgcggatcgc
      901 gccgatgtcg aggccgccac cgtcgtcttc gacggtgatc acgacctcgg cgccctcgcg
      961 ctgcaggcgc acgacgatct ggccggtctc ggccttgccg cgcgcgaggc ggttctcggg
     1021 caactcgatg ccgtgcacga cggcgttgcg cagcatgtgc tcgaacggcg gcagcatgcg
     1081 ctcgagcacc tgccggtcga tttcgcccga gccgccctcg atggtgagct cggccttctt
     1141 gccggcttcc tgcgccgcct ggcgcaccag ccgcgtgagc cgctgcgcat gacgctggaa
     1201 cgggaccatg cgcgtgcgca tcaggccgtt ctgcagctcg gtgatgatgc ggccctgctg
     1261 ctgcagcagg ttctggccgt ccttgttgac gctctcgagc aggctctcga tgctcgccac
     1321 gtcgctgacc gactcggcca gcgcgcgcga gagctgctgg atcgtcgaat agcggtcgag
     1381 ctcgagcgga tcgaaatccg cccggccatg cgtgtcgccc tggtgcttga acaggatctg
     1441 ggcctcggtc tcgagctcga gcttgcgcag ctgctcgcgc aggcgcgtga ccgtgcggcc
     1501 cagctcggcg aggtggaatt ccgtcgaggt catctgctgc tcgacccgcg cgcggaagat
     1561 gctcacctcg cccgcgttgt tcagcagctc gtcgagcagg tccgcgctga ctcgcgcgag
     1621 ttcgtggcgc tcgggcggcg gggccggctc gcggcccggc aacacgaacg gcacgcctgc
     1681 gggcggcagt tcgcgggtct cggcttccgc cacgatcggc acgtgctcgc caagcgcggg
     1741 ggccggcggc gcgccgactg cggtcgcggg ctccacgacc tccgcggcct cggcggcctc
     1801 cgcagggtac gcgacgaccg gcggctcctc tgcctccgga ccgaggatct ccgcgtgtgc
     1861 ggcaatgccc tcctgctccg ttggcatggg cggcgcggct cgctcgacga ccagcggcgc
     1921 aaccgctggc gcctcggtgg ctgccgcggc tgcgggcggt cccaccaccg ctggcgtctc
     1981 gggtgccgcc gcaggcgcgg ccgacgccga cgcgacggcg cgcacgcgct cgatcaggct
     2041 ccgcgccggg gcaatgcgct gtcccgcgtt cgccgcctcg cgcatgcggt gcaactcgtc
     2101 gagcgcggac tgcagcacgg cgatcgccgg ctcacccgac gcgatcgacc ccgcctcgag
     2161 cgaggcgagg aacgactcga tctcgtggct caggtcgccc atcgcgcgca ggccggccat
     2221 gcgcgcgccg cccttgagcg tgtgcagtac gcgcttcaac tcggtgatgc ggctgcggtc
     2281 ggcgcgatcg gccgtccagc ttgcaagcga cgaatccgcc tgctcgagca gttcgctcga
     2341 ttcctcgccg aagatcgccg cgatgtcggc gtcgaagtcg ggttcgattt ccgccgctgg
     2401 ctcgggcgcg acgggggccg agggcacctc gaagcgtgct gcggctggcg ccggcatctc
     2461 ggcaggaagc tcgggcatca acgggcgcgg ctcggcgatc tcgcgctccg cggccaccgc
     2521 ggcaggtgcc tcttgcggca cggcctccgc gagccgagca ggttcctccg cccaggactc
     2581 cgcggccgct gcctcgccgg cttctgcctg tcgggcgagc tccgcgtcca gcgcgcgatc
     2641 cagcgcgtgc catccggcga tcagccgccc ttgatccggg aaatagccgg tgtcctcctc
     2701 gacgtggtcg accacggtct cgaggctctg cacggtgtcg cgcagcagca gcaacccctc
     2761 ttccggcagt ccgcggccgt tgtcgtgcag cttgcggacg tagtcctcca tcggctcggc
     2821 gaccttgatg ccctgccgtg cgccggcagt cttggcgatg ccgctcaggg tgtggcaggc
     2881 gcggtacaac gcctcggtca ccgcgtacgg cgcgaccgct tcggcgcagc gcgcgatgta
     2941 ctcgcggaca gcctcgacgt gcccggccgt ctccttgcgg aagatgtcgc gcagcaccgg
     3001 gtccatggct gcgggctcgg gcaccttggc gggcgcggcg gcggccggcg gcatcgccgc
     3061 cgtctcgcga cccgcgagcg tgtcggcacg cgtcacgatg gccgccgcgt cgacctgcgc
     3121 cgcgcggccg tgctcgagct cgtcgaccag cttgggcatc gcggcgacgg cgtcgcgcac
     3181 gaccgcgacg atctcgggcg accggggcag tgtctggctg atgacacggt tcagcagcgc
     3241 ttcgatgctc cacgagaact cgctcacgcg ccgcgccccg accatgcggc cgctgccctt
     3301 gagcgtgtgg aatacgcgcc gcaggtcacg cagcgcggcg acatcgagcg ggttcgattc
     3361 ccactgcggg aacagctcgc acatcaccag gatgttctcg cgtgcctcct cgacgaacag
     3421 ctggcggaac tccgggtcga ccgcggtggc cgcttcctcg gcgggcgcag gtgccgcgac
     3481 ggctgccggc gccagcggcg ggaccggtgg cgggctcgcg gccgcctcgg gctgctgctg
     3541 ctgcggcggc gtgacgggcg gcgcggcggc gaccggttcc tcctgcgggc gcgccgggcg
     3601 caaggccacg accttggtcg gcgcctcctc caggtcgacg aggcagcgct ccgcgttctc
     3661 gagcatgtgc cacgggtcgg ggcgcccggc ctgcagcatc tccatgtagt actcgacggc
     3721 gacgatcgcg tcggcgaggc tgtcgagccg ctgcggagcg agcgcagcgg cgtccggccg
     3781 gacgatggcg ccgagcgcgc gcccgacgct ctccatgacc tccacggccc tctgcttccc
     3841 gagcatcagc agcccggccg tgatgccgcg gaccagctgc ggcacctggt cgaggccctg
     3901 cgcctcggac ggagccgcga gcgcgtgcgt cacggcttcc ttgatgcgcg ccatgttgac
     3961 gatgcactcg cgcaggacgg cgtcctggac ctgcttgaac tcctcgtcgg tcggctcgcc
     4021 gggttcgaca gcgccggcgg ccttgccttc cggcgtgccg ccgtcggaga cgatgagctt
     4081 cacgagctgg gcatcgaggc tgtcctcgac ctcgatcagc gcggaggcga tgtcgagcag
     4141 cgccgcatcg ccgggcggct cgcgccgctc cacgatgtgc gcgagcgact cgagcttctg
     4201 ctgcacccgg tcgcgcagcg cgcccaggcc cagcacgccg agcgtgtcgc cgatcttgcg
     4261 cagcatctcg agctgcggcg ccagctcgtc cacctgggtg gcaccgcggc ggacgaagat
     4321 gtccagcacg tccttgacgc gcgtcaggtc ctcgcggatc gccgcagcca cggtcttcat
     4381 gagccgcacg ctcggcgccg agagggtcgc ctgcgcctcg ctcaactgct cgtccacccc
     4441 gaggacctcg tccagccgga acgagcggcg cacggccagc acgcgcgcac cggaactgaa
     4501 cgagcgggcc acgtagaaca gcaggttgtt gagcaactcg accggcggcg tcgcggaata
     4561 gcgcgcttcg ccctgctcct gcagccgccg cagctcgcgg tcgacctggc cgagcaggcg
     4621 cttcaccgac acgttgccgt cgatgccgcc ctgctgcagc gactcgatca acgccgcgac
     4681 cacccaccac agctggaaca gcggctgggt cgtcgccgcc tgctcgaact ggccggcgat
     4741 gtcggacagc gccccaaggt tctcggccac ttgctcgttg cggatccagc cgagcagcgc
     4801 gagctgcagg cgcggccgca gcttgcgcgc cagctcggcg gccggcacct ggacgtcgcc
     4861 gcccggccgg gccggctcgt ccgagcgcag gttgagcatg agcagcgtgc cttccgagag
     4921 cagcgccccg ccacgcaccg cccggaggtc gttcagcagg ggcagcaacg cgagcggcac
     4981 gtcgcgcccg cccgaggcca cgcggtcgac gtaggccggc agctgctcca tggcgcgcat
     5041 cagcgcgtcg agcccgtcgg catccacctg gccgccccgc tcgtggctct ccaggtagtc
     5101 gacgacctgt tccatctcct cgccgagcag cgcgccgccg tagacctcgg ccatgcgcag
     5161 tgcgccccgc gcgacgtgca ggtgctcgcc gaaacggtgc agcgccgcgc gatcgccggc
     5221 gttctccgca taggcttcga gtgccgcacg ggcctcgttc agctcggccg cgacctcgcg
     5281 cacgaccagc tgcagcgagg cgtgcgtctc gtccggcgcg atggccagca tggcgttcat
     5341 gcccgggagc cctgcggcat cagccggccc ggccaccgac gcgccgcagc cgctcgacct
     5401 cgtcggccgc gagcgcctcc ggcttgagca ccgggtactc gccgctcacg ctcgcccccg
     5461 gacgctccgg caggcggaag cccgtgaccg acttgcgcag ctgcgccgcg agctccgcca
     5521 gcttgccgat cgagtcggac gtcgcggtcg tgctctcggc cgtctgcgag ctgatctccc
     5581 gcagcacctg catgttgcgc gagatgttct gcgacgaggc cgcctgctgg cgcgcgctcg
     5641 ccgagatgtt ctgcaccagg ctcgcgatct ggttggacac ctgctcgatc tcctcgagcg
     5701 cggcgcccgc gttctccgcg agcagcgcgc cgcccacgac gtcggtcgtg gtgcgctcca
     5761 tggagacgac cgcctcgttg gtgtccgcct ggatcgtccg caccagcacc tcgatctgct
     5821 tggtggcgtt ggcggcgcgc tcggcgagcc gctgcacctc gtcggcgacc acggcgaagc
     5881 cgcggcccgc ctcgccggcc atcgacgcct ggatcgacgc gttcagcgcc aggatgttgg
     5941 tctgctcggc gatgtcgttg atgagctcca cgatgttgcc gatttcctgc gagctctcgc
     6001 cgaggcgctt gatgcgcttc gacgtctcct ggatgttctc gcggatcgca ttcatgccgt
     6061 cgatcgtgcg gcgcaccgcg tcgccgccct tgtgcgcgat gtccaccgaa tggcgcgcga
     6121 cgtccgcgga gcgctcggca ttgcccgaga cctcctcggt ggacgaggcc atcgcggcga
     6181 tcgactcgga cgccgacgcg atctgcttcg actgcgccga ggacgccttg gccaggtggg
     6241 tcgacagcgc ctgcgtctgg cgggtcgccg cgtcaagctt gatcgcggaa tcgttgatcg
     6301 tcgtgaccag ttcgcgcagc gcctcgatgg cgtagttgat cgaatcagct attgctcccg
     6361 taatgtcctc ggtgacggtc gcctgcacgg tcaggtcgcc gtcggccaga ctcgagagct
     6421 cgtcgagcag ccgcagtatc gcctgctggt tgcgttcact ctgcacggcc tggtgctcgg
     6481 aggcgcgctg cacggtcgca atcgagcgct gcaggaacac gagcagcgcg agggccgcga
     6541 ccgcgagccc gagcagcccg agcggcaccc acgggctcgc gagccaggcc ctggccccgg
     6601 acggcgcagg tgcctgcagg cgcgcgaggc tcgccgacgc ctttgcgccc gactccgcga
     6661 gcgcgttgcc ggcagcctgc atctgctcga gcgagtcgcc gagcgcgatc accgcgcgca
     6721 cctgctgttg ctcgctgcgg aacacggccg cgaggaggtg cgccgcatcc gccgccggac
     6781 cctgcgccgc ggtgattccg agcggattgc tctcgccgac caggccgccg acgacctggc
     6841 ccatgtactc gaggctctcg gccagtcgcc ggcccgcggc ttcgacggat gccgtgccgt
     6901 cggccagggc cgagaaatcc tggtcgatcg cctgggcccg cagttcgaag cggtcgagct
     6961 gccggttgaa cgcgtcgggg cgcgccgccc ccatcacctt ggcgacgttg gcggtggcgg
     7021 ccacgacctg gggcgtgagc tcgcgcacgc cggctgccgc cttctgcgcc tcgagcgcgg
     7081 ccgcgcgccc gtcgagcacg gcctgcgatt gctcgagcat cgcgagccac gtggcccggt
     7141 cgcccggcag ccctgcttcc gacgtaccgg ccgctttcac cgccgcatcg agccgggccc
     7201 gcgaggcggc cagcgagtcg aacgcggcgg cggatccccg cacggccgcg ctggcctgca
     7261 gcggcatgcc ctgcgccagc gcactgagcg cggtcacctg ctcgccgacc ctggattgcc
     7321 cgccggtgag gcgccactgg agtgcgaaca ccaggcctgc cagcaccgcg gcaaggagcg
     7381 ccacccggat cagggtcggc tgcagtctgg cggcgtcgaa tctattgctc atcgttgcac
     7441 ccagggtcaa gttcggtctc gccacagttc ttcaggaggc cgcggcctgc aggaactgct
     7501 ggctctcgag caggccgcgc acgctgaaga cgggccagac ctcggcgccg cgcttgaagg
     7561 cgccggcgag gtagcgctcg cagcgcagca gcgtcggcgg gaggtccgac gagaattcac
     7621 cctcgtagaa cctgcggaat cccatcacct cgtccacgac gagtcccgcg gggatctcgc
     7681 ggtggttggc caccagcacg cgtgtcgcgc gggtcacgct ggtcgcgccc gacccgagga
     7741 aagcccgcac gtcgatcacc ggcatgagct gtccgcgcac gttcgacagg ccgcggatcc
     7801 agctcttggc gccgggcacg cgggtcaccg ccgccgggaa tcccatgacc tcgcgggtct
     7861 cctccctcgc gagcaggaag gcctcgccgc cgagccggaa cgcgatgccc acccactcgg
     7921 cccccgaggc agcggcctcc ggctggccct gcgccgcggc acgggcgcgg cggtccagct
     7981 cgcgcagcaa ctcgaatggc cgatcgcgca gcgacctcaa tgcacgggcg tcttccatct
     8041 caggtcgcga gcgccgcctg ggccttgcgc acgagctggt ccatcgcgac gggcttgacg
     8101 aggtagtcca cggcgccctg gcgcaggccc cagacccggt ccgactcctg ggacttcgag
     8161 gtcaccatga tcacgggaat ggcgcgggtc agcgggtcgt tggccagctc gcgcgtggcc
     8221 tggaacccgt tcagcccggg catgacgatg tccatgagga tcaggtccgg gtgcagctcg
     8281 cgggccttgc ggatgccctc gccgccgtcg gccgccgagg cggtgcgaaa gccgccctcc
     8341 tccagcgcct tctgcatgac gtgcacgtcc gtcggcgagt cgtccacgat cagtaccagt
     8401 gccacgggtc gggtcctccg gatcgtctgc ctcagcggcg cacgtgcgtt tcgatcgcgc
     8461 tcaggagttc ctcgcgcgtg aagggcttgg tcaggtagtg ctcggagccg acgatgcggc
     8521 cgcgggcgcg gtcgaacagg ccgtccttgg aggacagcat gatcaccggg atggtgcgga
     8581 acaccttgtt gtgcttgatc agcgagcagg tctggtagcc gtcgagccgc ggcatcatga
     8641 tgtccacgaa cacgatgtcg ggctggtggt cggccacctt ggagagcgcg tcgaaaccgt
     8701 ccaccgccgt gaagacctcg cagccctcgc gcgcgagcag cgtttccgcc gtgcggcgga
     8761 tggtcttgct gtcgtcgatc acgagcacct tgagccctgc aagtcgcggc cggctggcgc
     8821 tggcagcagt attttccatg ccgtcgaacc cccgaatggg ttgtgagcgt cgcagcctgg
     8881 acgctaaagc gcgcggcagt gctcgaggcg tggttttaac atattgggag gggtcccgcc
     8941 atcggccggt ttcgatacca tgaccggagt cacaggcgcc ccgaggaccg cgcggacatg
     9001 tggagcgaac gatgaacggc aagcccctgc gcgtcgcggt cgtcatggac ccgatcgagg
     9061 acatcaagcc ggcgaaggac acgacgctgg cgatgatgct cgccgcgcag cgacgcggct
     9121 gggaactcca ttacctcgag ttgggccacc tctggctgcg cgacggcgtc gccctcggac
     9181 gcgcgcaccc ggtcaccgtg cgcgacgacg cgagcgactg gtactcgcgt ggcgacgcgg
     9241 cggtccggcc gctcggcgag ttcgacgcga tcctgatgcg caaggacccg ccgttcgaca
     9301 ccgagtacat ctacgcgacc tacatcctgg agcgcgcgga gtctgccggc gccctggtcg
     9361 tcaaccggcc gcagggcctg cgcgacatga acgagaaggt ctacacggcc tggttccc
//
LOCUS       DAOMFD010000074         9412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2173_length_9412_cov_34.283317, whole genome shotgun sequence.
ACCESSION   DAOMFD010000074 DAOMFD010000000
VERSION     DAOMFD010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2173_length_9412_cov_34.283317"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..648
                     /locus_tag="PL152_05930"
     CDS             <1..648
                     /locus_tag="PL152_05930"
                     /inference="COORDINATES: protein motif:HMM:NF015424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HQR48853.1"
                     /translation="RALLARPRLLLLDEPVSALDESGTGEVLEALERVLAALPVPCLY
                     VSHDLREAARLADRMLWLDAGRVVADGPVSQVLSDPRLPFAQEEDAESVIAGRIEAVD
                     AGTGLASVAFDGGLLWIAASCSTVPGNAVRVRIRARDVSLALRRPEAISVLNILEGHV
                     LEVADARSAASQALVRVDVGGCRLLSRVTRRSVAELGLAPGVTVWALVKSAALLR"
     gene            671..1510
                     /locus_tag="PL152_05935"
     CDS             671..1510
                     /locus_tag="PL152_05935"
                     /inference="COORDINATES: protein motif:HMM:NF012472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HQR48854.1"
                     /translation="MPTTITIGGDLTVHRLGLGAMRITGAGIWGDPPDVAAARRLLRR
                     AVDLGINFIDTADSYGPDVSERIIAEALHPYPPGLAIATKGGIVRPAADQWVRDGRPE
                     HLREACLASLKRLRLERIDLYQLHAIDPRVPLEDSLGELARLQSEGLVRHVGVSNFGV
                     SELERARRVVNVVSVQNRYNVSDRSSDAVLAACERDGLAFIPWSPLTQAPRDAQGGAR
                     SALERWAGRRGVSFVQAATAWLLARSPAMLPIPGTASVRHLEDNAAAASLQFTPDELR
                     EIG"
     gene            complement(1654..3819)
                     /locus_tag="PL152_05940"
     CDS             complement(1654..3819)
                     /locus_tag="PL152_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HQR48855.1"
                     /translation="MQPARDLFSYRKHWAHRFGPAPFLPMSRDEMDELGWDSCDVILV
                     TGDAYVDLPSFGMAIVGRVLEAQGFRVGIIAQPDWHSTREFMKLGKPNLLFGVTGGNM
                     DSMVNRYTSDRRIRSDDAYTPHGVGGARPDRCVIVYSQRVREAYRDAPIVVGGIEASL
                     RRIAHYDYWSEKVRRSVLLDSKADLLVYGNAERQVCEIAHRLAAGTPVDEIRDLRGTA
                     FVTRGAPAGWVEIDSTHVDEPGPLAPPADPYAFAASGSAPATASPAAAVHALRSHHRV
                     ANADRARSVIRMPSYEQVVADPVLYAHASRVLHMESNPGNARCLVQRHGDVDVWLNPP
                     PIPLTTKEMDAVYELPYRRVPHPAYGGAKIPAFEMIRFSIAIQRGCFGGCTFCSITEH
                     EGRIIQNRSEDSVIREIETIRDTVPGFTGVISDLGGPTANMYRIACKSREIEAACRKP
                     SCVYPGICPNLDTDHSALIRLYRRARAVSGVKKVLIGSGVRYDLAIESPEYVKELAQH
                     HVGGYLKIAPEAISEGPLSKMMKPGVATYDRFKALFDKYSKEAGKEQYLIPYFIAAHP
                     GTTDEDMLELALWLKRNGFRADQVQAFLPSPMATATAMYHSGKNPLHKVTHSSPDVYI
                     PKGTRQRRLHKAFLRYHDPNNWPVLREALRRMGRADLIGNGRQHLVPTYQPIGTGRAH
                     EGKRRPFRTQHTGLPRAPRPPAGSGGNLKPRGPRARGRG"
     gene            3921..5228
                     /locus_tag="PL152_05945"
     CDS             3921..5228
                     /locus_tag="PL152_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase family
                     protein"
                     /protein_id="HQR48856.1"
                     /translation="MTDDPTEILKTPLRLACGVVLKNRLVKAAMTEGLAGPDGLPNER
                     HEQLYRTWARSGVGLLITGNVMVVGDHLERPGNVIADREFGPAERARFRAWAEAASED
                     GCQLWMQVSHAGRQTQKAVNPRPKSASDVQLGLPGGQFARPEPLTGEEIRDLVTRFAE
                     AAVLARETGFSGVQVHAAHGYLISQFLSPKANHRRDEWGGSLENRARFLRAIVAATRA
                     RVGRDFAVSVKLNSSDFQKGGFGPDDARQVVAWLEQDGVDLIELSGGTYEQPRMLELE
                     GLEPVVEIKLQDSTRQREEFFLGFASELKGVARVPLMVTGGFRTARGMSAAIREDGIA
                     AIGIGRPLCGDPLCTQRLLRDGADLPRYEKLLGNPRHFFGVNSPVKLVKAIASFSVMA
                     WYYDQIVLMSEGRPNDLDPACFRRFVALQRRQAAWLKARRSFH"
     gene            5271..5654
                     /locus_tag="PL152_05950"
     CDS             5271..5654
                     /locus_tag="PL152_05950"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2384 domain-containing protein"
                     /protein_id="HQR48857.1"
                     /translation="MVKRAEKASLEPEPSAVLTKAVTRAARLLGLTQRQLADTLGLSS
                     PTASRLFSGRYSLSPTRAKEWELAVLFVRLFRSLDALWGHEGTGREWLGTYNTALGAT
                     PLELIRSAAGLVRVVDYLDHARGRV"
     gene            5620..6351
                     /locus_tag="PL152_05955"
     CDS             5620..6351
                     /locus_tag="PL152_05955"
                     /inference="COORDINATES: protein motif:HMM:NF020387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HQR48858.1"
                     /translation="MTTWTTRAVASELRPARVDLWRGVEAQHVASTMALVDSVAEQAI
                     LEGLVEASKPPVPQEVASLGLHWLLFTPFRYAPPPGGSRFRGPNDPGVFYGAEQPRTA
                     CAEVGYWRWRHLTESPGLAAMPPRPQTLFRTPVAGRCIDLRAPPFDRDRAVWTHPADY
                     SGCQELGRTARAGAVQAIRYESVRDSRHGGCAAILDAAAFAVKAPVEQQGWLLTVTHA
                     RVTWQRSDPLNPGVFEFSAAGWQEK"
     gene            complement(6449..7171)
                     /locus_tag="PL152_05960"
     CDS             complement(6449..7171)
                     /locus_tag="PL152_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HQR48859.1"
                     /translation="MGRIFEVRKHAMFARWNRMAKQFARIGKEITIAVKKGGPDPNSN
                     PALRRTIQNARAVNMPKDKIEAAIKRASGRDAASFEEVMYEGYAPHGVAVLVECATDN
                     PTRTVASVRNAFNKHGGNLATTGSVAFQFKRMGVFRLNPEGIDQDDLELYLIDHGLEE
                     FGESTGEKGEPQLVARCAFEDFGTLQKALEDRGITPISAEHEYICQAPTELGETEATE
                     VLELIDKLEQDEDVQKVYHTLA"
     gene            7386..8003
                     /locus_tag="PL152_05965"
     CDS             7386..8003
                     /locus_tag="PL152_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR48860.1"
                     /translation="MSFYHHHLFPWLLDHALRHRSFGEPRRRTLAPASGRILEIGFGT
                     GLNLRYYPATVQRIEAIDPDVDLDRYSTPRIAASNIDVDFHHLDAEHLPFESERFDTI
                     VCTLTLCSIPDVVHALGEVRRVLKPDGQFLFLEHGLAPDASVARLQRRLTPAWRALAG
                     GCHLDRPVGSLLTAAGFRLDPVSDYYIEGLPRFVGFMTEGIARKD"
     gene            8104..9261
                     /locus_tag="PL152_05970"
     CDS             8104..9261
                     /locus_tag="PL152_05970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48861.1"
                     /translation="MQRFLQLAATAALLALGAGGCTFEASGDEGVSHQFGSDHFGVGA
                     ILNVTDPVAGDAFLVAGQVSTAAEIGGDLVAAGGEVSVGGNVGDDLYAAGGTVKVDAI
                     VNGNARVAGGDVSVGPATVIAGSAAVSGGRVAFEGNTHGYLKATGGTVTLSGQVHKDA
                     EVRAEDLVISPETRIAGRLVYHGPTAPEVPEGAVIAGGVEFHEVSPGTYLHETSSPVR
                     RAAHWVGSVLWHLGVFCVGALFVILLPGISSRAADTLRCEPLVSMGLGLGLLVCVPIV
                     LVMLCITVIGIPLALLLGTGYLLALFLGWIVVALFVGERGLSLVRGSQPVTTGLRLLA
                     LLLALVGLSYVRHIPYLGGLVVFLALIAGMGALVWQGWTRRPGAGSARVTA"
BASE COUNT         1389 a         3145 c         3306 g         1572 t
ORIGIN      
        1 cgggcgctgc tcgcgcgccc gaggctcctg ttgctcgacg agcccgtgtc cgcgctcgac
       61 gagagcggca ccggcgaagt gctcgaggca ctggagcgcg tccttgcggc gctgccggtc
      121 ccgtgcctgt acgtcagcca cgacctgcgc gaggccgcgc gactcgcgga tcgcatgctc
      181 tggctcgacg cgggccgcgt ggtcgcggac ggtccggtgt cgcaggtgct gagcgacccc
      241 cgcctgccgt tcgcgcagga agaggacgcc gagagcgtca tcgccggccg catcgaggcc
      301 gtggacgccg gtacgggact cgcgagcgtc gcattcgacg gcggactgct gtggatcgcg
      361 gccagctgca gcaccgtgcc tggcaatgcg gtccgcgtgc ggatccgcgc gcgcgacgtg
      421 agcctcgcac tgcgcaggcc agaggcaatc agcgtgctga acatcctcga gggccacgtg
      481 ctcgaggtgg cggacgcgcg atccgcggca tcgcaggccc tggtgcgagt cgacgtcggc
      541 gggtgccggt tgctttcgcg cgtgacgcgg cgctcggtcg ccgaactcgg actggcgccc
      601 ggtgtcaccg tctgggcgct ggtgaagagt gccgcgctcc tgcgctgagc gatcgcggta
      661 tatttgcccc gtgcccacca cgatcaccat cggcggcgac ctcaccgtcc atcggctcgg
      721 cctcggtgcg atgcgcatca ccggtgccgg catctggggg gacccgcccg acgtggctgc
      781 ggcgcgccgc ttgctcaggc gcgcggtaga cctcggcatc aacttcatcg acacggccga
      841 ttcgtacggc cccgacgtga gcgaacggat catcgccgag gcgctgcacc cgtatccgcc
      901 cggcctcgcg atcgcgacca agggcggcat cgtgcggccg gcggcggacc aatgggtgag
      961 ggatggccgc cccgagcacc tgcgcgaggc gtgcctcgcg agcctgaagc gccttcggct
     1021 cgagcgcatc gatctttacc agctgcatgc gatcgacccg cgcgtgccgc tcgaggactc
     1081 gctcggggag ctcgcgcgcc tgcagagtga gggacttgtg cgccacgtcg gcgtgtcgaa
     1141 tttcggtgtg tccgaactgg agcgggcgcg cagggtcgtc aacgtggtgt cggtgcagaa
     1201 tcgctacaac gtgtcggacc gttcgtccga cgccgtgctc gcagcctgcg agcgcgacgg
     1261 gctcgcgttc atcccgtggt cgccactgac gcaggcaccg cgcgacgcgc agggcggagc
     1321 gcgctcggcg ctcgagcgct gggccgggcg gcgaggcgtg tcgttcgtgc aggccgcgac
     1381 ggcgtggctg ctggcccggt cgcccgcgat gctgccgata ccgggcacgg cgagcgtgcg
     1441 gcacctcgag gacaacgcgg ctgccgcgtc gctgcagttc acaccggacg agttgcggga
     1501 gattggttga ccctacgacg gaggtgactt tcaatgagca agggcatgga tcgtaagaag
     1561 gaagggaaga agaaggcgca gaagtcgctc aaggagaagc gcgccgagaa gcgcgccaag
     1621 cgcagcggct gacccggccc gcagtaccgt cgatcaaccg cgtccgcgcg ctctcggccc
     1681 gcgcggcttg agattgccgc cggagcctgc gggcggacgt ggcgcgcgcg gcaggcccgt
     1741 gtgttgcgtg cggaacggcc ggcgcttgcc ctcgtgcgcg cgtcccgtcc cgatgggctg
     1801 atacgtcggc accaggtgct gcctgccatt gccgatcagg tcggcgcgac ccatgcgtcg
     1861 caaggcctcg cgcagcacgg gccagttgtt cgggtcgtgg taacgcagga acgccttgtg
     1921 cagccggcgc tgccgcgtgc ccttcgggat gtacacgtcc ggcgaggagt gcgtgacctt
     1981 gtgcagcggg ttcttgccgg agtggtacat cgcggtcgcc gtggccatcg gtgagggcag
     2041 gaacgcctgc acctggtcgg cgcggaagcc gttgcgcttg agccataggg cgagctcgag
     2101 catgtcctcg tccgtggtgc cggggtgcgc cgcgatgaag tacgggatga ggtactgctc
     2161 cttgccggct tccttcgagt acttgtcgaa cagggccttg aagcggtcgt acgtggcgac
     2221 gcctggcttc atcatcttcg acaaggggcc ttccgagatg gcttccggcg cgatcttcag
     2281 gtagccgccg acgtgatgct gcgcgagttc cttcacgtac tccggtgact cgatggcgag
     2341 gtcgtagcgc acgcccgagc cgatcagcac cttcttgacc ccggacaccg cgcgggcgcg
     2401 tcggtacaag cggatcagcg ccgagtgatc ggtgtcgaga ttgggacaga tcccggggta
     2461 gacgcacgag ggcttgcggc acgcggcctc gatctcgcgc gacttgcacg cgatccggta
     2521 catgttcgcc gtcgggccgc cgaggtccga gatcacgccc gtgaatcccg gcacggtgtc
     2581 gcggatcgtc tcgatctcgc ggatcaccga gtcctcggag cggttctgga tgatgcggcc
     2641 ctcgtgctcg gtgatcgagc agaacgtgca gccgccgaag cagccgcgct ggatcgcgat
     2701 cgagaaccgg atcatctcga acgcggggat cttcgcgccg ccgtacgccg ggtgcggcac
     2761 tcgccggtac ggcagctcgt acaccgcgtc catctccttc gtggtgagcg ggatcggcgg
     2821 cggattgagc cacacgtcta cgtcaccatg ccgctggacc aggcagcgcg cattgccggg
     2881 attcgactcc atgtgcagca cacgcgaggc gtgtgcgtag agcacgggat ccgcgacgac
     2941 ctgctcgtag gaaggcatgc ggatcacgct gcgagcgcgg tcggcgttgg cgacacgatg
     3001 atgtgaccgc aacgcatgca ccgcagcggc aggcgaagcc gttgcaggcg cggatccgga
     3061 agccgcgaac gcataagggt cggccggtgg tgcgagcggc cccggctcgt cgacgtgcgt
     3121 cgagtcgatc tcgacccagc cagccggtgc gccacgagtg acgaatgcgg tcccgcgcag
     3181 gtcgcggatc tcgtcgaccg gcgtgccggc cgcgaggcga tgcgcgatct cgcaaacctg
     3241 gcgctcggcg ttgccgtaca ccagcaggtc cgccttcgag tcgagcagca cggagcggcg
     3301 caccttctcc gaccagtagt cgtagtgagc gatgcggcgc agcgacgcct cgatgcctcc
     3361 gaccacgatc ggcgcgtccc tgtatgcctc gcgcactcgc tgcgagtaga cgatcacgca
     3421 gcggtctggg cgcgcgccgc cgacaccgtg aggcgtgtac gcatcgtcgc tgcggatgcg
     3481 gcgatccgag gtgtagcggt tcaccatcga atccatgttg ccgcccgtga cgccgaagag
     3541 gaggttcggc ttcccgagct tcatgaactc gcgggtcgag tgccagtcgg gctgcgcgat
     3601 gatcccgacc cggaagccct gcgcctccag gacacggccg acgatcgcca tgccgaagct
     3661 cggcaggtcc acgtaggcgt cgccggtgac caggatgacg tcgcaggaat cccagccgag
     3721 ctcgtccatc tcgtcccgcg acatgggcag gaatggcgcg gggccgaatc ggtgtgccca
     3781 gtgcttgcgg taggaaaaga ggtcgcgggc cggctgcatg ggcggcagtt tagccgcccg
     3841 gcgcagacgc ttgacgacac ttcggccgtg tgagggagtg agcgggcgca agtgacgcgc
     3901 ggtgcacgcg gaccgacccg atgaccgacg acccgaccga gatcctgaag accccgttgc
     3961 gactggcctg cggtgtcgtg ctcaagaacc gcctcgtgaa ggcggcgatg accgaaggcc
     4021 tggcgggccc ggacggcctg ccgaacgaga ggcacgaaca gctgtaccgc acctgggcac
     4081 ggagcggcgt cgggctcctc atcaccggca acgtcatggt ggtcggagac cacctcgagc
     4141 ggccgggtaa cgtcatcgcc gatcgtgagt tcgggccggc cgaacgcgcc cggttccgag
     4201 cctgggccga agcagccagt gaggacggtt gccagctctg gatgcaggtc tcgcatgccg
     4261 gacgccagac gcagaaggcg gtcaacccgc gaccgaagtc ggcgtccgac gtgcagctgg
     4321 gactgcccgg cggacagttc gcacgcccgg agccgttgac cggcgaagag atccgcgacc
     4381 tggtgacccg ctttgccgag gccgcagtgt tggctcggga aaccggcttc agcggcgtcc
     4441 aggtccatgc ggcccatggc tacctcatct cgcagttcct gagtccgaag gcgaaccacc
     4501 gccgggacga atggggcgga agcctcgaga accgggcgcg attcctgcgc gcgatcgtcg
     4561 cggccacgcg tgcgagggtc ggtcgcgact tcgccgtaag cgtcaagctc aacagctccg
     4621 acttccagaa gggcggcttc ggccccgacg atgcgcgcca ggtggtcgcg tggctggagc
     4681 aggacggggt cgacctgatc gagctgtccg gcggaacgta cgagcagccc cgcatgctcg
     4741 agctcgaggg gctggagcca gtggtggaaa tcaagctgca ggacagcacc cgccagcgcg
     4801 aggagttctt cctcggcttt gcgtccgagc tcaagggcgt cgcccgcgtg ccgctcatgg
     4861 tgaccggcgg ctttcgcact gcgcgtggca tgtccgcggc aatccgcgag gacggcatcg
     4921 ccgccatcgg catcggccgg ccgctgtgtg gcgacccgct gtgcacgcaa cgactgttgc
     4981 gcgacggagc cgacctgccg cgctacgaga agctgctcgg gaatccgcgc cacttctttg
     5041 gcgtgaacag cccggtcaag ctggtcaagg ccatcgcgag cttctcggtc atggcctggt
     5101 actacgacca gatcgtgctg atgagcgagg gtcggcccaa cgacctggac ccagcctgtt
     5161 tcaggcgctt cgtcgcactt cagcgtcggc aggcggcgtg gctcaaggcg agacgctcgt
     5221 ttcattagaa tgattttgca aaaaatgttt cactggaata tgatcggtgc atggtcaagc
     5281 gagccgaaaa ggcgtccctg gagccggagc cgagcgccgt cctgaccaag gcggtgacgc
     5341 gtgctgcgcg cctcctgggt ctcacccagc gccaattggc cgataccctc ggcttgtcca
     5401 gtccaactgc gtctcgcctc ttctcaggcc ggtacagcct ctcgccgacg cgggccaagg
     5461 aatgggaact ggccgtgctc ttcgtgcggc tgtttcgttc gctcgatgcg ctctggggcc
     5521 acgaaggcac gggccgcgag tggctcggca cgtacaacac ggcgctcggt gccacacccc
     5581 tcgagctgat acgcagcgcc gcggggctgg tgcgcgtcgt tgactacctg gaccacgcgc
     5641 gcggtcgcgt ctgaactccg gccggcgcgc gtcgatctct ggcgcggcgt cgaggcgcag
     5701 cacgtcgcgt ccacgatggc gctcgtcgac tcggtcgcgg agcaggcgat cctcgagggc
     5761 ctggtcgagg cgtcgaagcc gccggtgccg caggaggtcg cgtcgctggg cctgcactgg
     5821 ctgctgttca cgccattccg ctacgcgccg ccgcccggcg ggtcacgctt tcgtgggccg
     5881 aacgaccccg gcgttttcta cggcgccgag caaccccgca cggcgtgtgc cgaagtaggg
     5941 tactggcggt ggcgacacct caccgagtcg ccaggcctcg ccgcaatgcc gccgcgcccg
     6001 cagacactct tccggactcc ggtggccggt cgatgcatcg acctgcgcgc gccgcctttc
     6061 gatcgtgatc gagcggtctg gacgcacccg gccgactact cgggctgcca ggaactcggg
     6121 cggaccgcgc gcgctggcgc ggtgcaggcc attcgctacg agtccgtgcg agattcccgg
     6181 cacggcggct gcgccgccat cctggacgcc gcggcattcg cggtgaaggc gccggtcgag
     6241 cagcaggggt ggctgctcac ggtcacccat gcacgcgtga cgtggcagcg cagcgatccc
     6301 ctgaatcccg gtgtattcga gttcagcgcc gcgggctggc aggaaaaata ggggacattc
     6361 accgatttct cgctccggac gggcgctccg cgagcaccct cgctgcgcgg gaatccggtg
     6421 cgcgggagtc cggtgaatgt cccctcattt acgcgagcgt gtgatacacc ttctgcacgt
     6481 cctcgtcctg ctcgagcttg tcgatcaact cgagcacctc ggtcgcctcg gtttcgccca
     6541 gttccgtcgg cgcctggcag atgtactcgt gctcggcgga gatcggcgtg atcccgcggt
     6601 cctcgagcgc cttctgcagc gtgccgaaat cctcgaaggc gcaacgggca acgagttgcg
     6661 gctcgccctt ctcgccggtg ctctcgccga attcctcaag gccgtggtcg atgaggtaca
     6721 gctcgaggtc gtcctggtcg atgccttccg ggttgaggcg gaacacgccc atgcgcttga
     6781 actggaaggc cacgctgccg gtggtcgcga ggttgccgcc gtgcttgttg aaggcgttgc
     6841 ggacgctggc gacggtgcgc gtcgggttgt cggtcgcgca ctcgacgagc accgccacgc
     6901 cgtgcggagc gtagccctcg tacatcactt cctcgaagct ggcggcgtcg cgaccggacg
     6961 cacgcttgat cgccgcctcg atcttgtcct tcggcatgtt tacggcgcgc gcgttctgga
     7021 tcgtgcgccg caaggccggg ttcgagttgg ggtcgggtcc gcccttcttc accgcgatgg
     7081 tgatttcctt gccgatgcgg gcgaactgct tcgccatgcg gttccagcgc gcgaacatcg
     7141 cgtgcttgcg gacttcgaag atgcgaccca tgggaacgac cttgctcgga gggcgcgcaa
     7201 tctacctgag cgcgcgtggc gggccaactg gccgggcggg aatattaaca actggcaaaa
     7261 caaaccggcg atgcgttatg gtctgcacgg gtaaattgtg aatttcatct gaactcgaaa
     7321 atggccggat ccgggacccg tgcaggctcc ttgcgatcac cgatcaacga cgcgctggca
     7381 ctcccttgag cttctaccac caccatctct ttccgtggct gctcgaccac gcgctccgcc
     7441 accggtcctt cggcgagccg cggcggcgta ccctcgcgcc ggcgtccggg cgcatcctcg
     7501 agatcgggtt cggaacggga ctcaacctgc gctactaccc ggcgacggtg cagcggatcg
     7561 aagccatcga cccggacgtc gacctcgacc ggtactcgac gccgcggatc gcggcctcga
     7621 acatcgacgt ggatttccac cacctcgatg ccgaacacct gcccttcgag agcgaacgct
     7681 tcgacacgat cgtgtgcacg ctcacgctgt gctcgatccc cgacgtggtg cacgcgctcg
     7741 gagaggtgcg ccgcgtcctg aagccggacg gccagttcct gttcctcgag cacgggctcg
     7801 cgccggacgc gagcgtcgca cggttgcagc gtcgcctgac accggcttgg agagcactgg
     7861 ccggtggttg ccacctggac cggcccgtgg ggagcctgtt gaccgcggcc ggtttcaggc
     7921 tcgacccggt cagcgactac tacatcgagg gactgccgcg cttcgtcggt ttcatgaccg
     7981 aaggcatcgc ccgcaaggat tgagccgttc gcagagcggg accacgttgc cctaaactgg
     8041 ccgcgatggc ctgcaacact gccggtgccc gtcgcgacgg gtgccgctcg tgaggtatcg
     8101 gcaatgcagc gtttcctgca acttgcggca accgccgccc tgcttgcgct cggcgccggc
     8161 ggatgcacgt tcgaggcgag cggcgacgag ggcgtgtcgc accagttcgg ttccgaccac
     8221 ttcggggtgg gcgcgatcct gaatgtcacc gacccggtcg ccggcgatgc cttcctggtg
     8281 gcgggccagg tcagcaccgc cgccgagatc ggcggcgacc tcgttgccgc gggtggcgaa
     8341 gtgtcggtgg gcggcaacgt cggcgacgac ctctacgccg cgggcggcac cgtcaaggtc
     8401 gatgcgatcg tgaatggcaa cgcgcgcgtc gccggcggcg acgtgtcggt cggtcccgcg
     8461 acggtgatcg cagggtccgc agccgtgtcc ggcggtcgcg tcgctttcga gggcaacacc
     8521 cacggctacc tgaaggcgac cggcggcacc gtgacgctgt ccggacaggt gcacaaggat
     8581 gccgaggtgc gcgccgagga cctcgtcatc tcgccggaga cccggatcgc cggtcgcctc
     8641 gtctatcacg gaccgaccgc gcccgaggtg cccgaaggcg cggtcatcgc gggcggcgtc
     8701 gaattccacg aggtgagccc cggcacctac ctgcacgaaa cgtcgagccc cgtgcgccgg
     8761 gcggctcact gggtcggcag cgtgctgtgg cacctgggcg tcttctgcgt gggcgcgttg
     8821 ttcgtgatcc tgcttcccgg catctcgtcg cgagccgcgg acaccctccg gtgcgaacca
     8881 ctcgtctcga tgggcctggg cctggggctg ctcgtgtgcg tgccgatcgt gctcgtcatg
     8941 ctgtgcatca ccgtgatcgg catcccgctc gcgctgttgc tcgggacggg ttacctgctc
     9001 gcgctgttcc tgggctggat cgtcgtcgcg ctgttcgtcg gggagcgcgg cctgtcgctg
     9061 gtgcgcggtt cgcagccggt cacgaccggc ctgcggctgc tggcactgct gcttgcgctc
     9121 gtcggcctgt cgtacgtgcg gcacatcccg tatctaggcg gcctcgtcgt gttcctcgcg
     9181 ctcatcgctg ggatgggcgc gctggtgtgg cagggatgga cgcggcgccc cggtgccggc
     9241 agcgcgcgcg tgaccgcatg acgtactggc cccacctgct ggccgtgatc gtcggcgcgg
     9301 cgttgaccgt ccagatcggc atgaactcca cgctgcgctt cggcctcgga tcgccgatcc
     9361 tcgcgacgat cgtcaacttc gccgtcgggc tggcggcgct ggtcgccgtc gc
//
LOCUS       DAOMFD010000075         9209 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2253_length_9209_cov_38.297138, whole genome shotgun sequence.
ACCESSION   DAOMFD010000075 DAOMFD010000000
VERSION     DAOMFD010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9209)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9209)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9209
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2253_length_9209_cov_38.297138"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..969
                     /locus_tag="PL152_05975"
     CDS             <1..969
                     /locus_tag="PL152_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQR48862.1"
                     /translation="FANYAVDDCDFLIALGARFDDRVAAVPDRFAPNAKHIAHIDADA
                     SEINKVKRVDWSHVGLLPQALTALLDRARRTGLKRDWTAWHAHLDGLRRKYAMNYDRD
                     SRLIQPYYVIEEINRLTRGEAIIATGVGQHQMWAAQYFDFREPRLWLTSGSMGTMGFG
                     LPAAIGAQFARRDKLVIDVDGDASMRMNLGELETVTTYDLPVKVIVLNNFGDGMVKQW
                     QKLFFKGRLSASDKSLHKKDFVRAAQADGFRYAVRLDRKEDVPRVVKEFVEFDGPAFL
                     EVIIDPDAGVYPMVGPGQSYDEMITGEWIPSRGKGGTQEADATGMF"
     gene            complement(991..1626)
                     /locus_tag="PL152_05980"
     CDS             complement(991..1626)
                     /locus_tag="PL152_05980"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HQR48863.1"
                     /translation="MKLWHVLATLLFLPLGAAAAPGDTPPADQAADNAPYNVLGDPQA
                     PVSIIEYSDLQCPFCARFARETFPRIKSEYIDTGKVRYAVADFPLPMHPQAVPAAIAL
                     RCAGEQGQFWTYREQLYKDQMLLVPSLYELMAQQLGLDVTRFNDCRNDPQQRAAVEAA
                     YQYGARNKIASTPTFVVGRVVDGQDRIEVIEGAKPIDVFRKTIDALLEAAQ"
     assembly_gap    1774..1873
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1877..2914)
                     /gene="murB"
                     /locus_tag="PL152_05985"
     CDS             complement(1877..2914)
                     /gene="murB"
                     /locus_tag="PL152_05985"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010478.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HQR48864.1"
                     /translation="MSEAFTILRDAPLRDANTLRVAATARWLATVRDPSALPDVLGRP
                     EFAGLPVLVLGEGSNVLFATDFAGLVLRPSWRRARIIGDESGAALVRAEAGQGWDALV
                     DWSLAQGLAGLENLALIPGLVGAAPIQNIGAYGAEVGEFITAVEAFDQRLGATARVEH
                     ARCGFGYRDSVFKREPDRWIITAIELRLPRSRAPNLGYSGVREELAAMGHDQPTPAQV
                     AQAVRRLRRRKLPDPAVIGNAGSFFKNPVVPAALAEALRAAHPGMPVHPADGDARKLS
                     AAWLIEACGWRGFRDGDAGISAQHSLVLVNHGRASGLQLLDLARRVADSVEQRFGVRL
                     EPEPRIVGARF"
     gene            3010..>3329
                     /locus_tag="PL152_05990"
     CDS             3010..>3329
                     /locus_tag="PL152_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48865.1"
                     /translation="MSPRGTGAAAIAGPDEAATFLAANPDVEAVQLAITDANGIGRGK
                     NIAREELEALYRSGRNVAGSILGLDVTGEDVEETGLVWEVGDADLCCRPVAGSLTRAP
                     WLERP"
     assembly_gap    3330..3429
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3430..4196
                     /locus_tag="PL152_05995"
     CDS             <3430..4196
                     /locus_tag="PL152_05995"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48866.1"
                     /translation="RTLMSEYAPGQFEITLEHRDDALRAVDEAVLFKRAVRGVARRHG
                     CIACFMAKPFAHLAGTGMHLHASLADAGGANAFASDDPAGTPLLRHAIGGLRQTLADG
                     MAVFAPHANSYRRFRAMSYAPVAPTWGVNNRSVSLRIPAGPPASRHVEHRVAGADANP
                     YLVAALVLAAMLKGIEGRIDPGPPIVGNGYEQAPRGELPTHWQAALDRAAASEFLAWA
                     LGPEFLKVFLALKRRECEKFGALVSDRDYEWYLDST"
     gene            4334..5209
                     /locus_tag="PL152_06000"
     CDS             4334..5209
                     /locus_tag="PL152_06000"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR48867.1"
                     /translation="MSVTRTSRAAARNASLLARKSQPVTVHLRKMYVDCRYGQLHVHT
                     AFPSSGGFDELTPLVCVHPGATSGRVFRPLLADLGTDRSVYAPDLPGHGESDAPDSPP
                     SMTEYSAAVGDLLDTLRLRQVDVLGYQGGSLAAIELAIARPEQVRRVVLASVPVFDAK
                     DREAFNSRPWPPRAREDGSHLAEEWQRIRRARGSHAVPSRMSEDLSSALRAGDSAVWG
                     PSAAANYPAGERLPLLRQPTLVLRPHDEYWDMGARVDVLIRESRRLDLPDQDGGLLDV
                     AAGDVARYLRDFLDR"
     gene            complement(5246..5677)
                     /locus_tag="PL152_06005"
     CDS             complement(5246..5677)
                     /locus_tag="PL152_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48868.1"
                     /translation="MARIELLMSVLAGTSGGMAGLVWSRLVSAAWLARAAGSSQVSQD
                     AEPASRQVAAAVLFAGGGAALGFLFWLGWGLISLVGASWVSVGMLYGALAWAGVALPL
                     LGVLALRLRGLGGAALVLAVEWLVTCEAIGLSCALAWHRYA"
     gene            5801..7321
                     /gene="ilvA"
                     /locus_tag="PL152_06010"
     CDS             5801..7321
                     /gene="ilvA"
                     /locus_tag="PL152_06010"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014578420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - L-threonine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0009097 - isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HQR48869.1"
                     /translation="MQESYIERILKARVYDVAVETPLERAPRLSRRLGNAVLFKREDL
                     QPVFSFKVRGAYNKISHLPEAVARRGVICASAGNHAQGVALAGRRRGIPAVIVMPQTT
                     PNIKVQSVLDLGGEVVLHGDDFDHAYEHARELARDRGLTFIHPFDDPDVIAGQGTVGM
                     EILRQHSGPVDAIFVPVGGGGLVSGIAAYVKSLYPQVRIVGVEPEDAASMHDSLRAGM
                     RVTLERVGIFADGVAVRRVGEETFRLAQQYVDEVVLVNTDEICAAVQDIFEDNRSIAE
                     PAGALAVAGIKKYVAREGCAERTLVAVNSGANVNFDRLRHVAERADIGAQREALLAVE
                     IPERPGSFLEFCRLLGRRSVTEFNYRYDDSAVARIFVGLALHGGSHEKAALLGLVSTS
                     GFRVLDMSDDELAKLHVRYMVGGHAPRLANERLLRFEFPERPGALLRFLEAVGTRWNI
                     SLFHYRNHGSDYGRVLAGVQVPSADSDEFARHLADLGYAYTEETANPAYRMFLGRG"
     gene            7458..8192
                     /gene="cmoA"
                     /locus_tag="PL152_06015"
     CDS             7458..8192
                     /gene="cmoA"
                     /locus_tag="PL152_06015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HQR48870.1"
                     /translation="MSADEKDQVFAQPIDRIADFKFGEDVASVFDDMLLRSVPFYAEQ
                     QRMIGELATDFAVAGTNVYDLGCSTGTTIIELGRVLPQDVRFVGMDNSEDMLVKCRAK
                     LDEHGFKRPVDLVCADLNQGCEVANASLVLMILTLQFIRPLQRERLLTDVFRGMNENG
                     ALILVEKVLGEESLFNRLFIEHYYDFKRRSGYSDLEITQKREALENVLVPYKLLENRE
                     MLLRTGFRHVDVFFKWYNFCGIVAVK"
     gene            8192..8899
                     /locus_tag="PL152_06020"
     CDS             8192..8899
                     /locus_tag="PL152_06020"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: protein motif:HMM:NF016107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HQR48871.1"
                     /translation="MVALGNLLYRTRLYVFPLACLLVFLPGPRIWQDDLVAAVLGFAI
                     ALAGQVVRAGTIGLVYIVRGGKNRQVYAKDLVTDGVFGHSRNPMYVGNLLILSGMAVT
                     SNCWSTVLVAIPLTAFIYRAIVAAEENYLRGRFGADFDAYCARVPRFVPRLQGLGETF
                     SSMDFRWRRVVVKEYGTPTAWVLVWCAIVAWNLTRHDQAALRPWALPMLEIVAILAVI
                     GWATARILKKTGRVVAD"
     gene            complement(8928..>9209)
                     /locus_tag="PL152_06025"
     CDS             complement(8928..>9209)
                     /locus_tag="PL152_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48872.1"
                     /translation="WTRNCRNQAYRLGDRAWGLQFHLEADEALINRWLDSESGQAEID
                     RHWSLRRIARIRTATRQHLPVAQPLSDRVFSEFVRLVGPQSRGLVLPSR"
BASE COUNT         1375 a         3151 c         3130 g         1353 t
ORIGIN      
        1 ttcgcgaact acgccgtcga cgactgcgat ttcctcatcg cgctcggcgc gcgcttcgac
       61 gatcgcgtgg ccgcggtgcc cgaccgcttc gcgcccaacg cgaagcacat cgcgcacatc
      121 gacgccgacg cctccgagat caacaaggtc aagcgcgtcg actggagcca cgtcgggctg
      181 ctgccgcagg cgctcaccgc gctgctggac cgggcacggc gcacgggact gaagcgcgac
      241 tggaccgcgt ggcacgcgca cctggacggc ctgcggcgga agtacgcgat gaactacgac
      301 cgcgacagcc gcctgatcca gccgtactac gtcatcgagg agatcaatcg cctgacgcgg
      361 ggcgaggcga tcatcgcgac cggcgtgggc cagcaccaga tgtgggccgc gcagtacttc
      421 gacttccgcg agccgcggct gtggcttacc tccggcagca tggggacgat gggtttcggc
      481 ctgcccgcgg cgatcggcgc ccagttcgca aggcgcgaca agctcgtgat cgacgtcgac
      541 ggcgacgcga gcatgcgcat gaacctgggc gagctcgaga ccgtcacgac ctacgacctg
      601 ccggtcaagg tgatcgtgct gaacaacttc ggcgacggca tggtcaagca gtggcagaag
      661 ctgttcttca agggccgcct gtcggcgagc gacaagtcgc tgcacaagaa ggacttcgtc
      721 cgggccgcgc aggcggacgg attccgctac gcggtgcggc tcgaccgcaa ggaggacgtg
      781 ccgcgcgtcg tgaaggaatt cgtcgagttc gacgggcccg cgttcctcga ggtcatcatc
      841 gaccccgacg cgggcgtcta cccgatggtc gggcccggcc agtcctacga cgagatgatc
      901 accggcgagt ggatcccgtc ccgcggcaag ggcggcaccc aggaagccga cgccaccggg
      961 atgttctgag gcgcgcgcgc cgggccctcg ttactgggcg gcctcgagca gcgcgtcgat
     1021 cgtcttgcgg aacacgtcga tgggcttcgc gccctcgatg acctcgatcc tgtcctggcc
     1081 gtccacgacc cgaccgacga cgaacgtcgg cgtcgaggcg atcttgttcc gggccccgta
     1141 ctggtaggcc gcctccacgg ctgcgcgctg ctgcggatcg ttgcggcagt cgttgaagcg
     1201 cgtgacgtcg agcccgagtt gctgcgccat cagctcgtaa agagaaggca cgagcagcat
     1261 ctggtccttg tacagctgct cacggtatgt ccagaactgc ccctgttcgc ccgcgcagcg
     1321 caaggcgatc gcggcgggca cggcctgcgg gtgcatgggc agggggaagt cggccacggc
     1381 atagcggacc ttgccggtgt cgatgtactc gctcttgatc cgtgggaacg tctcccgcgc
     1441 aaagcgcgcg cagaacgggc attgcaggtc gctgtactcg atgatcgaga cgggtgcctg
     1501 cggatcgccg agcacgttgt acggcgcgtt gtccgccgcc tgatcggccg gcggcgtgtc
     1561 ccctggcgcc gcggccgcgc ccaggggcag gaacaggagt gtcgcgagga cgtgccagag
     1621 tttcatggtg cgactccgcg cgcggtggag gtgaatcgag gcccgtgacg ctaacattgt
     1681 ggggccgggg ccagccactc cccgcgggcg aggcaacatg agatacctgc atacgatggt
     1741 gcgcgtgtcc gacgtggacg cgtcgctgcg gttnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1861 nnnnnnnnnn nnngcttcag aacctcgcgc cgacgattcg cggttccggt tcgagccgca
     1921 cgccgaagcg ctgctcgacc gagtcggcga cgcgccgcgc caggtccagc agttgcagtc
     1981 cgctcgcgcg accgtgattg accagcacca gggagtgttg cgccgaaatc ccggcgtcgc
     2041 cgtcgcgaaa cccgcgccag ccgcacgctt cgatgagcca ggccgcggag agcttgcgcg
     2101 cgtccccgtc ggccgggtgc accggcatgc ccggatgcgc ggcccggagg gcttccgcga
     2161 gcgcagccgg gaccaccggg ttcttgaaga aactgccggc attgccgatg accgccgggt
     2221 cgggcagctt gcgacgtcgc agccgccgca ccgcctgcgc gacctgggcc ggcgtcggct
     2281 ggtcgtgtcc catggcggcg agctcctcgc gcacgccgga gtaccccagg ttcggcgcgc
     2341 gtgacctcgg gagccggagt tcgatcgccg tgatgatcca gcggtcgggc tcgcgcttga
     2401 acacgctgtc ccggtagccg aacccgcagc gcgcgtgttc cacacgcgca gttgcaccca
     2461 gccgctggtc gaaagcctcg accgctgtga tgaattcccc gacctcggcg ccgtacgcac
     2521 cgatgttctg gatcggcgcg gcaccgacca gccccgggat cagggcgagg ttctcgagtc
     2581 cggccagtcc ctgcgccagc gaccagtcca ccagcgcgtc ccagccttga ccggcctcgg
     2641 cgcggacgag cgcggcgcca ctttcgtccc cgatgatgcg tgcgcggcgc caggacggcc
     2701 gcaagacgag ccccgcaaag tcggtggcga acagcacgtt gctgccctcg ccgagcacca
     2761 gcaccggcag tcctgcgaat tccggccgcc cgagcacgtc gggcagggct gaaggatcgc
     2821 gcaccgtcgc gagccagcgc gccgtcgccg cgacgcgcag cgtgttcgcg tcccgaagcg
     2881 gcgcatcgcg caggattgtg aaggcttcgc tcatggggga tcccatgcag acggtaggcc
     2941 cggcattgtg gcattgcccg gccggtgctt gcttgccact cgcgacgtgg ttccgtcact
     3001 atcgcccgca tgagtcctcg aggcacagga gcagcggcga tcgcaggacc ggacgaggcc
     3061 gccacgttcc tcgcggccaa tccggacgtc gaggccgtgc agctcgcgat cacggacgcg
     3121 aacggcatcg gccgtggcaa gaacatcgcg cgcgaggaac tcgaggcgct gtaccgcagc
     3181 gggcgcaacg tcgcgggctc catcctcggg ctcgacgtca cgggcgagga cgtcgaggag
     3241 accggcctcg tctgggaagt cggcgacgcg gacctgtgct gccggcccgt cgcgggctcg
     3301 ctgacgcgag cgccgtggct cgagcggccn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnt gcgcacgctc atgtccgaat acgcgcccgg ccagttcgag atcacgctcg
     3481 agcaccgcga cgacgcattg cgcgcggtcg acgaggcggt gctgttcaag cgcgcggtgc
     3541 gtggcgtggc ccggcgccac ggctgcatcg cgtgcttcat ggccaagccg ttcgcgcacc
     3601 tggcgggcac cggcatgcac ctccacgcga gcctcgccga tgcgggcggc gcgaatgcgt
     3661 tcgcgagcga cgatcccgcc gggacgccgc tgctccggca cgcgatcggc ggcctgcgcc
     3721 agacgctggc cgacggcatg gcggtgttcg cgccgcacgc caattcgtat cgccgtttcc
     3781 gcgccatgag ctacgccccc gtcgcgccga cctggggcgt caacaaccgt tccgtgagcc
     3841 tgcggattcc ggccggcccg cccgcgagcc gacacgtcga gcaccgtgtc gccggcgccg
     3901 acgccaatcc gtacctcgtc gccgcgctgg tgctcgcggc gatgctgaag ggcatcgaag
     3961 gacgcatcga tccggggccg ccgatcgtgg gcaatggcta cgagcaggcg ccgcgcggcg
     4021 agctgccgac gcactggcag gccgcgctcg accgagccgc tgcgtcggag ttcctcgcgt
     4081 gggcgctcgg ccccgagttc ctgaaggtgt tcctcgcgct caagcggcgc gagtgcgaga
     4141 aattcggcgc gctcgtgtcg gaccgcgatt acgagtggta tctcgattcg acgtgacgct
     4201 tgcgacataa cggccggcgc ggccccggcg cgcattaagt cagctcggga ggcacgtttg
     4261 tgagagttga atcaacgtga ccccggcgcg cggcgcggac aatccgtccg cattgccgcc
     4321 gaacccgagg tccatgtcag tgacacgcac cagccgcgcc gccgcacgca acgccagtct
     4381 gctcgcaagg aagtcccagc ccgtgaccgt ccacctgcgc aagatgtacg tcgactgccg
     4441 ctacgggcag ctgcacgtgc acacggcctt cccctcgagc ggcggcttcg acgagctgac
     4501 gccgctcgtg tgcgtgcacc cgggcgcgac gtcggggcgg gtgttccgcc cgctgctcgc
     4561 cgacctcggc acggaccgca gcgtgtacgc gccggacctc ccgggccacg gggaatccga
     4621 tgcgccggac tcgcctccgt ccatgaccga gtactcggcc gcggtcggcg acctgctcga
     4681 cacgctgcgg ctgcggcagg tcgacgtgct cggctaccag ggcggttcgc tggcggcgat
     4741 cgaactggcg attgcccggc cggagcaggt gcgccgcgtg gtgctggcca gcgtgccggt
     4801 gttcgacgcc aaggaccgcg aggcgttcaa ttcgcggccc tggccgccgc gcgcacgcga
     4861 ggatggttcg cacctcgccg aggagtggca gcgcatccgc cgcgcgcggg ggtcgcacgc
     4921 cgtcccgtcg cgcatgagcg aggatctctc gagtgcgctg cgcgccggtg actcggccgt
     4981 ctggggcccg tccgccgccg cgaactatcc ggcgggagag cgactcccgc tgctgaggca
     5041 gccgacgctg gtgctgcgtc cgcacgacga gtactgggac atgggagcgc gagtcgacgt
     5101 cctgatccgc gagtcccgtc ggctggacct cccggaccag gacggcggac tgctcgacgt
     5161 ggccgcgggc gacgtggcgc gctacctgcg cgacttcctg gatcgctgag tcagcggcgg
     5221 tcgctgccgc gcagggctcg cgccgtcacg catagcggtg ccaggccagc gcgcagctga
     5281 gcccgatcgc ctcgcaggtc accagccact ccaccgcgag caccagcgcg gcgccgccga
     5341 ggccgcgcag cctgagcgcg agaacgccga gcagcggcag cgcgacgccg gcccacgcca
     5401 gcgctccgta cagcatcccg acggaaaccc agctcgcgcc gacgagcgag atcaggcccc
     5461 agcccagcca gaacaggaac cccagggccg ctcctccccc ggcaaacagc accgccgcag
     5521 cgacctgccg tgacgcgggc tcggcatcct gcgagacctg cgaactgccc gccgcgcgtg
     5581 ccagccaggc tgcggacacg agccggctcc agaccaggcc agccattccg ccactggtcc
     5641 cggcaagaac gctcatcagc agttcgatgc gagccataca gacgccgcgg tgaagagagc
     5701 gagggagtat gcggcgcgct gctgcaaacg agaagcagcc tcaggcggcc tgcagcgagc
     5761 tttcctgcta acgtagctcg tcccgccagg ccgtcacccc atgcaagaga gctacatcga
     5821 acgcatcctg aaggcgcgcg tctacgacgt cgcggtcgag acgccgctcg agcgggcccc
     5881 gcgcctgtca cgccggctcg gcaatgccgt gctgttcaag cgcgaggacc tgcagccggt
     5941 cttctcgttc aaggtgcgcg gcgcctacaa caagatctcg cacctgcccg aggccgtggc
     6001 gcggcgtggc gtcatctgcg cgtcggcggg caaccatgcc cagggagtgg cgctcgccgg
     6061 gcgtcgccgc ggcattcccg cggtcatcgt gatgccgcag accacgccga acatcaaggt
     6121 gcagtccgtg ctcgatctcg gcggcgaggt cgtgctgcac ggcgacgatt tcgaccacgc
     6181 ctacgagcac gcgcgcgagc tggcccgcga ccgcgggctg acgttcatcc acccgttcga
     6241 cgacccggac gtgatcgcgg ggcagggcac ggtcggcatg gagatcctgc gccagcactc
     6301 cggcccggtg gatgcgatct tcgtgccggt cggcggcggc ggcctggtgt ccggcatcgc
     6361 ggcctacgtg aagtcgctgt acccgcaggt ccgcatcgtc ggcgtcgagc ccgaggatgc
     6421 cgcgagcatg cacgactcgc tgcgcgcggg catgcgcgtg acgctcgagc gggtcggcat
     6481 cttcgccgac ggcgtggcgg tgcggcgggt cggcgaggaa acattccgtc tcgcgcagca
     6541 gtacgtcgac gaggtcgtcc tcgtgaacac cgacgagatc tgcgcggccg tgcaggacat
     6601 cttcgaggac aaccgcagca tcgcggagcc ggccggcgcg ctggcggtcg cgggcatcaa
     6661 gaagtacgtc gcccgggagg gctgcgcgga gcgcacgctg gtggcggtca attccggcgc
     6721 caacgtcaat ttcgaccggc tgcgccacgt cgccgagcgc gccgacatcg gcgcccagcg
     6781 cgaggcgttg cttgccgtcg agatcccgga gcgcccaggc agcttcctcg aattctgccg
     6841 cctgctcggg cgacgcagcg tcaccgagtt caactaccgc tacgacgact cggccgtcgc
     6901 gcgcatcttc gtcgggctcg ccctgcacgg cgggtcccac gagaaggcag cgctgctcgg
     6961 gctcgtgtcc acgtcgggct tccgcgtgct cgacatgagc gacgacgaac tggcgaagct
     7021 gcacgtccgg tacatggtcg gcggccatgc gccgcgcctc gccaacgagc ggctgctgcg
     7081 gttcgaattc ccggaacggc cgggcgcgtt gctccggttc ctcgaggcgg tcggcacgcg
     7141 ctggaacatc agcctgttcc actaccggaa ccacggctcg gactacggcc gggtgctcgc
     7201 gggggtgcag gtgccgagcg cagattccga cgagttcgcc cggcacctgg cggacctcgg
     7261 ctacgcgtac accgaggaga ctgcgaaccc cgcctaccgg atgttcctgg ggcgcggctg
     7321 agcgcagaat ccccagtcgg gtcaaggcct tgtgacctgg ctgggggccg cccgaattgt
     7381 aattggcggc cggcgcgact aaggtacgcg cctttctttg ggggccgggt gcgccagcgc
     7441 gccccgcgag gtttgccatg agcgccgacg agaaagacca ggtcttcgca cagcccatcg
     7501 accggatcgc cgacttcaag ttcggcgagg acgtcgcgtc ggtcttcgac gacatgctgc
     7561 tgcgctccgt gccgttctat gccgagcagc agcgcatgat cggcgaactc gcgacggact
     7621 tcgcggtcgc gggcaccaac gtctacgacc tcgggtgctc gaccggcacc acgatcatcg
     7681 agctcgggcg cgtgctgccg caggacgtcc gcttcgtcgg catggacaac tccgaggaca
     7741 tgctggtcaa gtgccgcgcc aagctggacg agcacggttt caagcgaccg gtcgacctcg
     7801 tctgcgccga cctcaaccag ggctgcgagg tcgcgaatgc gtcgctggtg ctgatgatcc
     7861 tgaccctgca gttcatccgg ccgctgcagc gcgagcgcct gctcaccgac gtcttccgcg
     7921 gcatgaacga gaacggggcc ctgatcctgg tcgagaaggt gctgggcgag gaatcgctgt
     7981 tcaatcgcct gttcatcgag cactactacg acttcaagcg gcgcagcggc tacagcgacc
     8041 tcgagatcac gcagaagcgc gaggccctcg agaacgtgct cgtgccctac aagctgctcg
     8101 agaaccgcga gatgctgctg cgcaccgggt tccggcacgt ggacgtgttc ttcaagtggt
     8161 acaacttctg cggcatcgtc gcggtgaagt gatggtcgcg ctcggcaatc tcctctaccg
     8221 cacgcgcctc tacgtcttcc cgctcgcctg cctgctggtg ttcctgccgg gtccgcggat
     8281 ctggcaggac gacctggtcg ccgcggtgct ggggttcgcg atcgcgctgg ccgggcaggt
     8341 cgtaagggcg ggcaccatcg gactcgtgta catcgtccgc ggcggcaaga accggcaggt
     8401 ctatgccaag gacctcgtca cggacggcgt gttcggccac tcgcgcaacc cgatgtacgt
     8461 cggcaacctg ctgatcctct cgggcatggc ggtgacgtcg aactgctgga gcaccgtcct
     8521 ggtcgcgatt ccgctcaccg ccttcatcta ccgggccatc gtcgccgcgg aggagaacta
     8581 cctgcgcggg cggttcggcg ccgacttcga tgcgtactgc gcgcgggtgc cgcgcttcgt
     8641 gccgcgcctc cagggcctcg gcgagacctt ctcgagcatg gacttccgct ggcggcgcgt
     8701 cgtcgtcaag gaatatggaa cgccgaccgc gtgggtgctc gtctggtgcg ccatcgtcgc
     8761 ctggaatctc acgcggcacg accaggcggc gctgcgtcca tgggccctgc cgatgctcga
     8821 aatcgtcgcc atcctggccg tgatcggctg ggcgacggcg cggatcctga agaagacggg
     8881 gcgagtggtc gccgactgag cgggcggcgg cgcgagcgtc tgcgtcgcta ccgcgacggc
     8941 agcacgagtc cccgtgactg cggacccacc agccgcacga actccgagaa cacccggtca
     9001 ctgagcggtt gcgcgacggg caggtgttgc cgcgtcgccg tgcggatgcg cgcgatgcgt
     9061 cgcaggctcc agtgccggtc gatctcggcc tgccccgact cgctgtccag ccagcggttg
     9121 atcagcgcct cgtccgcctc gaggtggaac tgcagtcccc acgcgcggtc gccgagccgg
     9181 taggcctggt tgcggcagtt gcgcgtcca
//
LOCUS       DAOMFD010000076         9175 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2265_length_9175_cov_27.564254, whole genome shotgun sequence.
ACCESSION   DAOMFD010000076 DAOMFD010000000
VERSION     DAOMFD010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9175)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9175)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9175
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2265_length_9175_cov_27.564254"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1351)
                     /locus_tag="PL152_06030"
     CDS             complement(<1..1351)
                     /locus_tag="PL152_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR48873.1"
                     /translation="MDRFWLKSYPPGVPADVDVSQYRSLPHLMTESFRKYAGRDAFIS
                     MDHALTYADVDRLSSAFGAWLQAKGLERGARVAIMMPNILQYPVALAGVLRAGYVVVN
                     VNPLYTPRELEHQLKDSGAEAIVILENFAAVLQQVVARTSLKHVVVTSFGDLLGFPKG
                     AVVNFALRRIRHMVPAYSLPGHLRFMDAIHEGLRLELEPVATGPDDIAFLQYTGGTTG
                     VAKGAVLLHRNLIANLLQCEAWLGPALVDTSRGPVPEQFVYVCALPLYHAFALVSNCL
                     FGMRVGAVNVLIANPRDIHQTVKDVRRHPFHVLPAVNTLLNALAENEEFRKLDFSSLR
                     ITIGAGMAVHKAVADKWFALTGCQVVEGYGLTETSPAAVVNPIVGTVWNGSIGLPIPG
                     TEVAILDDAGRPVPLGQPGEIAIRGPQVMAGYWHRPDETAKVMTPDGFFKSGDIGIMD
                     "
     gene            complement(1616..2149)
                     /locus_tag="PL152_06035"
     CDS             complement(1616..2149)
                     /locus_tag="PL152_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48874.1"
                     /translation="MSSAYIPKNGRLDKRRHFIRAMSKTIHGRPLTQSERTLLDSIFE
                     ALLRGEDVSDLTGISRPRTRRSADRIYVALHYLCLTQLMHTPADVAWRVVGDAWGLKK
                     RDVRWIIVRNHEPAQIALQHFTGAPDRLLRICERHARGTVSERDGSGQYGSSQGPGDD
                     IVPMLARLFREQPPTPH"
     gene            complement(2284..>3096)
                     /locus_tag="PL152_06040"
     CDS             complement(2284..>3096)
                     /locus_tag="PL152_06040"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HQR48875.1"
                     /translation="LVLAATATTALANPPNARGNDSYLALGDSVVFGYITADGYAYGN
                     ANNFIGYPAYLGLGLRFDIANASCPGETTSGFIDAAAPDNGCRPYRANFPLHVNYSST
                     QLDYAMNYLQTHKHTRLVTIGLGANDAFLLQSACNGDLSCIQSGLPTLLGSIYANMNT
                     ILANLRSTGYRGVLMVVNYYSLDYSDPVQTGFTNAVNQYLAAAAAANGAVVADVFTAF
                     QQVAALGGGKTCVVGLLNANPADQWTCDVHPSQSGQQLLAKTIAATYSRKRH"
     assembly_gap    3097..3196
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3472..3966
                     /locus_tag="PL152_06045"
     CDS             3472..3966
                     /locus_tag="PL152_06045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48876.1"
                     /translation="MSATQSLTIADALKSLREDIQAENTLIASRVTWYVTSQAFLLTA
                     YATSWNAGFVWPAFFHRILPLAAIVLSVLILASIYAATWAQDVYIREQAALIARIKSG
                     SPMSEAETMALKAYERTMVANRTNRAGRVIGGRIHALVRITPLVLPVAFSVLWLYAYC
                     FAPL"
     gene            4088..4954
                     /locus_tag="PL152_06050"
     CDS             4088..4954
                     /locus_tag="PL152_06050"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HQR48877.1"
                     /translation="MKTRWSRWLVGLAGAIATAGSVSSPAYGADAAPALAGAAAAAAG
                     ATAQLTESQRRAADLLKTMTDTLAGLKSFSVVFRDGYDVVQASGQKIEFGETRTVTVV
                     RPNLMRVEEVSSDGLRDLALFGGHEISVLDADANVYAQVPQPGTLDESLVYFVRELRM
                     RMPLALLFSTQLPEALAGRVKTIDYVESTDIVGVPTHHIAGRTDNVDFQFWVAATGKP
                     LPVRVVITYVQSPGEPQFWAAFARWNENPKVAADTFKFTPPAGARKIAFAVQVRPGAP
                     GQSPAASGEVQP"
     gene            4951..5655
                     /locus_tag="PL152_06055"
     CDS             4951..5655
                     /locus_tag="PL152_06055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48878.1"
                     /translation="MSTGPGMRTMLMVLVASTALVLLAVGDAYARGGRGGGGGGRGGG
                     GFSRSGPAAGGGFSQRGGGAPSSRDVSRPAPSQRPAASQQPSRPSDGARPPAGERPPG
                     ERPPAGERPPGERPPGERPPAGERPPPPPVYLPPPPPPGYYYGGGWDYYDNDDFFVGV
                     AVGAVVGGAAAEASEEEETTTTTTTVSSGMVSVLPCQPTVTNVEGVTYYQCGTQYYVE
                     AYGGSGPIFMPVSPPQ"
     gene            5652..6563
                     /locus_tag="PL152_06060"
     CDS             5652..6563
                     /locus_tag="PL152_06060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR48879.1"
                     /translation="MSEAKVAALTLGRGALRFSARSCGQGPLVLCLHGFPDNAGSWRH
                     QLPVFADAGYRAVAVTLRGYEPSSVTSTGGYGQAELAADVIAFLDGLGADRAHLVGHD
                     WGAAIGYAAAAAAPDRFASLTTLAVPHPGRFLTGILGHPRQLRLSWYMFFFQLRGIAE
                     RTVSRDDFEFIRRLWRDWSPGWTAPPDVLESVVDTLRQPGVLGAALGYYRSALSPGSL
                     PLTRSARRAACFPVPVPTLAISGERDGCIDSRVFRDMMRREDFPAGLEVATIPEAGHF
                     VHQEQPAAVNARLLEWFRRHPCANVNA"
     gene            6611..7108
                     /locus_tag="PL152_06065"
     CDS             6611..7108
                     /locus_tag="PL152_06065"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQR48880.1"
                     /translation="MRDAKTFTGRCTCGAVRYELTSRPLFVHCCHCRWCQRETGSAFV
                     MNAMIEAERVLLLQGAPLVVLTPTLSGKGQKIARCPACHVALWSNYAGAGDAIRFVRV
                     GTLDEPDRLPPDIHIFTESKQPWVVLPAAAPAVPEYYDREQYWPKDSLERRARLLAAT
                     TRGGT"
     gene            7105..7983
                     /locus_tag="PL152_06070"
     CDS             7105..7983
                     /locus_tag="PL152_06070"
                     /inference="COORDINATES: protein motif:HMM:NF020039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HQR48881.1"
                     /translation="MTTRQATVLTSGLYFGEGPRWHQGRLWFSDFHEHAVKSVDPAGQ
                     MRTELELDGPPSGLGWLPDGRLLVVAMHRRHLLRLDPDGLKLHADLSSVAAFHTNDMV
                     VDGHGRAYVGNFGFDLDAALKARGADGVLAEHPTARLARVDPDGKVHVAAGDLHFPNG
                     CVITPDGRTLIVAETLALRLTAFDIAPDGSLSNRRVWATLGMRAPDGICLDANGHVWI
                     ANAIGPECVLFAPGGEIVETVQTSQPCFACMLGGPDRRTLYMMTAPSSVAEVAAQARH
                     GAIETAAVATPGAGWP"
     gene            complement(8030..>9175)
                     /locus_tag="PL152_06075"
     CDS             complement(8030..>9175)
                     /locus_tag="PL152_06075"
                     /inference="COORDINATES: protein motif:HMM:NF005760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HQR48882.1"
                     /translation="TVAADAARAVQAACAAPGGVATLILPADTAWTEASHAAGPLPPI
                     APQPVASAAVDEAAQLLKNGKRTAILLRGRALRGAGLEAAGRIAAKTGARLLSDTFAP
                     HSELGAGRVPVERLPYFAEQIVDALAKVEQLILVGSKPPVSFFAYPGKRSWCAPDGCR
                     FAQLAQEHEDGVQALEALDAALGGPAPGVARVPLGLPDLPRGALNAVSAAQAIAHLTP
                     EHVIMADESATSGMPLAMMMARARPHDHLALTGGSIGLGLPLALGAALACPDRKVVCP
                     HGDGGAAYTMQALWSMARERQDVTVVIYANRSYAILNIELQRVGASAIGPKALSMLDL
                     HNPEMNWTKIAQGLGVEASRAETAEQFSDQYASAMRQRGPRLIEAMI"
BASE COUNT         1374 a         3025 c         3194 g         1482 t
ORIGIN      
        1 cgtccatgat cccgatgtcg cccgacttga agaacccgtc gggcgtcatg accttggcgg
       61 tctcatcggg gcggtgccag tagcccgcca tcacctgcgg accgcggatc gcgatctcgc
      121 cgggctgtcc cagcggcacc ggacggcccg cgtcgtccag gatcgcgacc tcggtgcccg
      181 ggatcgggag cccgatcgag ccgttccaga ccgtgcccac gatcggattg acgaccgcgg
      241 caggggacgt ctcggtcagg ccgtagcctt cgaccacctg gcagccggtc agcgcgaacc
      301 acttgtcggc caccgccttg tgcaccgcca tgcccgcgcc gatcgtgatg cgcaggctcg
      361 agaagtcgag cttgcggaac tcctcgttct cggccagcgc gttcaggagc gtgttgaccg
      421 ccggcagcac gtggaacggg tgcctgcgga cgtccttgac cgtctggtgg atgtcgcgcg
      481 ggttagcgat cagcacgttc accgcgccga cgcgcatccc gaacaggcag ttcgagacca
      541 gcgcgaaggc gtgatagagc ggcagtgcgc acacgtagac gaactgctcc ggcaccggcc
      601 cgcggctggt gtccacgagc gccgggccca gccacgcttc gcactgcagg aggttcgcga
      661 tcaggttccg gtgcagcagc acggcaccct tcgccacgcc ggtcgtcccg cccgtgtatt
      721 gcaggaaggc gatgtcgtcg ggccccgtcg caaccggctc gagctcgagc cgcaggcctt
      781 cgtggatcgc gtccatgaac cgcaggtgtc ccggcagcga gtacgccggc accatgtgcc
      841 ggatgcgccg gagcgcgaaa ttgaccaccg cccccttcgg gaaccccagc aggtcgccga
      901 acgacgtgac gacgacgtgc ttcagcgacg tgcgcgcgac gacctgctgc agcacggccg
      961 cgaagttctc gaggatgacg atcgcctccg cgccggaatc cttcagctgg tgctcgagtt
     1021 cgcgcggcgt gtagagcggg ttgacgttga cgaccacgta gccggcccgc aacactcccg
     1081 cgagtgcgac cggatactgc aggatgttcg gcatcatgat cgcgacgcga gcgccgcgct
     1141 cgaggccctt tgcctgcagc catgcgccga atgcactcga cagtcggtcc acgtctgcat
     1201 acgtcagcgc gtggtccatg gaaatgaatg cgtcgcggcc cgcgtacttg cgaaacgact
     1261 ccgtcatgag gtgcggcagc gaacggtact gcgacacgtc gacatccgcc ggcacgccgg
     1321 gcggatagct cttcagccag aacctgtcca taaatccccc tctgtcccga ccctgagtcc
     1381 caacactggc cggcggcgtg gatcgcgcgt cggcccgatg ctcagaatct gcgccggtag
     1441 gggaaggcct tcaattcgta gatggtgaag cgccgccgcg agactcaggc ggaggcgcgc
     1501 gagacgcggg cgggcgatag gctagcgatt tatacctgcc gtcgtagccg cacttcccga
     1561 tgcgtctcgg cgtgcaggga ccggcggacg gcagcccgag cgtcggtggc ctgcctcagt
     1621 ggggcgtcgg cggctgctcc cggaacaggc gggccagcat cggaacgatg tcgtcgcccg
     1681 gaccctgtga cgatccgtac tgcccggatc cgtcgcgttc ggacaccgta ccgcgcgcgt
     1741 gtcgctcgca gatgcgcagc aggcgatccg gggcaccggt gaagtgctgc aatgcaatct
     1801 gggcgggctc gtggtttcgc acgatgatcc agcgcacgtc gcgcttcttc agtccccagg
     1861 catcgccgac gacgcgccac gcaacgtcgg ccggcgtgtg catcagttgc gtcaggcaca
     1921 ggtagtgaag cgcgacatag atgcgatcgg cggagcggcg cgtgcggggt cggctgatgc
     1981 ctgtcaggtc ggagacgtcc tcgccgcgca gcagcgcttc gaagatggag tcgagcaggg
     2041 tgcgctcgga ctgcgtcagg gggcgtccgt ggatcgtctt cgacatcgcg cgtatgaagt
     2101 gccgccgctt gtcgaggcga ccgttcttcg gaatgtaagc gctgctcatg tcgcggcctg
     2161 ccgtccgtgc gtgcaccgct ggctcaggtc ctttagaaca cagttgcgca gtgcttcaca
     2221 agcccttgac gacataacgc aggtcgggac ggcgcagcgc cgtcccgacc ctcgccgaca
     2281 gtctcagtgc cgcttgcggg agtaggttgc cgcgatggtc ttcgccagga gctgttgccc
     2341 ggactggctg gggtgaacgt cgcacgtcca ctggtcggcc ggattggcat tgagcaggcc
     2401 tacgacgcag gtcttgccgc ctccgagcgc cgccacctgc tgaaatgcgg taaagacgtc
     2461 ggcgacgacc gccccgttcg cggccgctgc ggcggcgaga tactggttga cggcgttcgt
     2521 gaatcccgtc tgcacggggt cggagtagtc cagcgaatag tagttgacca ccatcaggac
     2581 gcctcggtac cccgtcgacc gcaggttcgc caggatcgtg ttcatgttgg cgtagatcga
     2641 gccaagcagc gtcgggaggc cggactggat gcacgagaga tcgccattgc acgcgctctg
     2701 cagcaggaag gcgtcgttgg ccccgaggcc gatcgtcacc agccgtgtgt gcttgtgggt
     2761 ctggaggtag ttcatcgcat agtcgagctg cgtcgacgag taattgacgt gcagcgggaa
     2821 attcgcgcga tacggacggc aaccgttgtc gggcgccgcc gcatcaatga acccgctggt
     2881 ggtctcgccg ggacacgatg cgttggcgat gtcgaagcgc aggccaagtc cgaggtacgc
     2941 cggatacccg atgaagttgt tggcgtttcc gtacgcgtac ccatccgccg tgatgtagcc
     3001 gaacacgacg ctgtcgccga gagcgaggta gctgtcgttg ccgcgcgcgt tgggtgggtt
     3061 ggccagggcg gtcgtggccg tcgcggccag gaccagnnnn nnnnnnnnnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnncaag gggtactcca tcccgacgac ccgctcggcg cccactgcga
     3241 gccgttcgtc gcaggaccct ggatgaattc gagtatcgaa cctgccaggc aatggcctgg
     3301 ggttcgtact gcggcggggc aggcgccgac gttccacggt cgcgctggcg catccgtaga
     3361 aatgaccctg tcgccgaccg gtgcccgcgt cgcagacttg cagcatgggg ccgcaagccg
     3421 gggaacgccg tccctgggcg aatccggaat cggccatccg gcgggggtga catgtccgcg
     3481 actcagtcat tgacgatcgc cgacgcgctg aaatcgctac gcgaggacat ccaggcggag
     3541 aacacgctga ttgccagtcg cgtgacctgg tacgtgactt cgcaggcatt cctgctcacc
     3601 gcgtacgcga cctcatggaa cgcgggcttc gtgtggccgg cgttcttcca tcgcatcctg
     3661 ccgctcgcgg cgatcgtgct ttccgtgttg atcctggcgt ccatatatgc cgcgacgtgg
     3721 gcgcaggacg tctacatacg tgagcaggcc gccctcattg cgcgcatcaa gtccgggtcg
     3781 ccgatgagcg aggccgagac catggcgctg aaggcctacg agcggaccat ggtcgccaat
     3841 cgcaccaacc gcgcaggccg tgtgatcggt ggccggattc atgcgctggt gcggatcacg
     3901 ccgctggtgc tcccggtcgc attctcggtg ctgtggctgt acgcctactg ctttgcgccc
     3961 ctttgacggc ggtgccgcgg cgccgacccc ggtgggggga taacggtttc gccaggctgc
     4021 catacaatca gagctgccgg tggctgctct cgcctgcgca agcgcctggt cgcgaggtga
     4081 tcgcaacatg aagacccgat ggagtcgttg gttggtcggc ctggcgggcg cgattgcgac
     4141 cgcaggatcg gtgtcctccc cggcgtacgg tgccgatgcc gcccccgcgc tggccggcgc
     4201 tgccgcggct gcggcgggag ccaccgcgca gctgaccgag tcgcagcggc gggcggccga
     4261 cctcctgaag accatgacgg ataccctggc cgggctgaag tcgttcagtg tcgtgttccg
     4321 ggatggctac gacgtcgtcc aggcgtcggg gcagaagatc gagttcggcg agacgcgcac
     4381 ggtgacggtg gtccgtccga acctgatgcg cgtcgaggag gtgtcgagcg acggcctgcg
     4441 tgacctcgcc ctgttcggcg gccacgaaat ctcggtgctg gatgcggatg ccaacgtcta
     4501 tgcgcaggtc ccgcagcccg gcacgctgga cgagtcgctg gtgtatttcg tacgggagct
     4561 gcgcatgcgg atgccgcttg cactcctgtt cagcacgcag ctgcccgaag cgctggccgg
     4621 ccgggtgaag accatcgact acgtcgagag cacggacatc gtcggcgtgc cgacgcacca
     4681 cattgccgga cgaaccgaca atgtggactt ccagttctgg gtcgccgcga ccggcaagcc
     4741 actgccggtc cgtgtcgtca tcacctacgt gcagtcgccg ggagagccgc agttctgggc
     4801 ggcattcgcg cgctggaacg agaacccgaa ggttgcggcc gacacgttca agttcacgcc
     4861 gcccgccggt gcccgcaaga tcgcgtttgc cgtccaggta cggcccgggg cgcccggcca
     4921 gtcgcccgct gccagcgggg aggtgcagcc atgagcacgg gaccgggcat gcggacgatg
     4981 ttgatggtgc tggtcgcctc cacggcgctc gtgctgctcg cggtcggcga tgcctatgct
     5041 cggggcgggc gaggaggtgg cggcggcgga cgaggaggtg gcggtttctc gcgatcgggc
     5101 ccggccgccg gcggcgggtt ttcgcagcgc gggggcggtg cgccttcgag ccgcgacgtg
     5161 agtcggcctg caccgtcgca aaggcccgcg gccagccagc agccatcacg tccttcggac
     5221 ggcgcgcgac ccccggctgg tgagcgtccc ccgggcgaac gtcccccggc cggtgaacgg
     5281 ccgcccggcg aacgtcctcc gggagaacgt cccccggcgg gcgaacggcc acctccgcca
     5341 ccggtctacc tgccaccgcc gccgccgccg ggttactact acggcggggg ctgggactac
     5401 tacgacaacg acgacttctt cgtcggcgtc gccgtgggcg ctgtggtcgg tggggctgca
     5461 gccgaggcgt ccgaggagga ggaaaccacg acgacgacga ccacggtcag ttcgggcatg
     5521 gtctcggtcc tgccgtgcca gccgaccgtg acgaatgtgg agggcgtgac gtactaccag
     5581 tgcggaacgc agtactacgt ggaggcctac ggcggcagcg ggccgatctt catgcccgtg
     5641 tcaccaccac agtgagcgaa gcgaaagtcg cggcgctgac gctgggccgc ggcgcgctgc
     5701 gcttcagcgc ccgatcctgc ggtcagggcc cgctggtcct gtgcctgcac gggtttccgg
     5761 acaacgccgg ctcctggcgg caccagttgc cggtcttcgc cgatgccggt taccgcgcgg
     5821 tcgccgtgac gctgcgcggc tacgagccga gctcggtgac ctcgaccggc ggctatggcc
     5881 aggcggagct cgcggccgac gtgatcgcgt tcctcgacgg gctcggggcg gaccgggctc
     5941 acctcgttgg tcacgactgg ggcgcggcca tcggctatgc ggcggccgcg gcagcaccgg
     6001 atcgattcgc gagtctcacg accctggccg tgccgcaccc gggtcgtttt ctcacgggga
     6061 tcctggggca tccacgccag ctgcggctgt cctggtacat gttctttttc cagttgcggg
     6121 gcattgccga gcgaaccgtg tcgcgcgatg acttcgagtt catccgccgg ctgtggcgcg
     6181 actggagccc gggatggacg gcgccgccgg acgtgctcga gagcgtggtc gataccctgc
     6241 ggcagccggg cgtgctcggt gcggcgctgg gctactaccg gtcggcgctg agcccgggca
     6301 gcctgccgct gacccggtcc gcgcgtcgcg ccgcatgctt cccggtgccg gtgccgacgc
     6361 tggctatcag cggcgaacgc gacggatgca tcgactcccg ggtgtttcgc gacatgatgc
     6421 gccgggagga tttcccggcc ggcctggagg tcgcgaccat cccggaggcc gggcacttcg
     6481 tgcaccagga gcagcctgcg gcagtcaatg cgcgcctcct cgagtggttt cgtcgtcacc
     6541 cctgtgcgaa tgtgaatgcc tgatcgcccg gttcccgcag tcctgacggg caggagccac
     6601 gatggtcgag atgcgagacg cgaaaacgtt caccggccga tgcacctgtg gcgcagtacg
     6661 ctacgaactc acgagccggc cattgttcgt ccactgctgt cactgtcgct ggtgccagcg
     6721 cgagaccggg tccgcattcg tgatgaatgc gatgatcgaa gctgagcgcg tcctgctcct
     6781 gcagggtgcg ccgctggtcg tgctgacgcc gacgctgagc ggcaagggcc agaagatcgc
     6841 gcgctgcccg gcgtgccacg tcgcgctctg gagcaactat gccggcgccg gtgacgcgat
     6901 ccgattcgtt cgcgtcggca cgctcgacga gccggatcgg ttgccgcccg acatccacat
     6961 cttcacggag tcgaagcagc cgtgggtcgt gctgccagcg gcggctccag cggttcctga
     7021 gtactacgat cgcgagcagt attggccgaa ggacagcctc gagcgccggg cccggctgct
     7081 ggctgcaaca acgcggggtg gcacatgacg accaggcaag cgaccgtcct cacgagcggt
     7141 ctctacttcg gcgagggccc gcggtggcac cagggccggc tgtggttcag tgacttccac
     7201 gaacatgcgg tgaagtcggt cgatcccgcc ggacagatgc gcactgagct cgaactggac
     7261 ggtccgccgt cgggactggg ctggttgccg gacggacgat tgctggtggt cgccatgcac
     7321 cgccggcatc tgctgcgact cgacccggac ggactcaagc tgcacgcgga cctgtcctcg
     7381 gtcgcggcct ttcacacgaa cgacatggtc gtcgacgggc acggccgcgc gtacgtcggc
     7441 aatttcggct tcgacctcga cgcggccctc aaggcgcggg gcgccgatgg cgtgctggcg
     7501 gagcacccga ccgcgcggct ggcacgggtc gatcccgatg gaaaggtcca cgtcgccgcc
     7561 ggcgacctcc atttcccgaa tggctgtgtc atcacgccgg acggccggac gctgatcgtc
     7621 gcggagaccc tggcattgcg cctcacggcg ttcgacatcg cccccgacgg atcactctcg
     7681 aaccggaggg tctgggccac gctcggcatg cgggcgccgg acggaatctg cctcgatgcc
     7741 aatgggcatg tctggatcgc gaatgcaatc gggccggagt gcgtgctctt cgcgccaggt
     7801 ggcgagatcg tcgagacggt gcagacgagc cagccgtgtt tcgcctgcat gctcggcggc
     7861 ccggatcgcc gcactctcta catgatgacc gcgccgtcgt ccgtcgcaga ggtggcagcg
     7921 caggcacggc acggcgcgat cgaaacggct gcggtcgcga cgcccggggc gggctggccc
     7981 tgagcgcagc gcagcggaca tcggcgcggt cgaactgata gcccgcgcgt cagatcatcg
     8041 cttcgatcag gcgcggtccg cgctgtctca tcgcggacgc atactggtcg ctgaactgct
     8101 cggcggtctc cgcacggctg gcctcgacgc cgaggccctg cgcgatcttc gtccagttca
     8161 tttccgggtt gtgcaggtcg agcatcgaca gcgccttggg tccgatcgcg ctggcgccca
     8221 cgcgctgcag ctcgatgttg aggatcgcgt aggagcggtt ggcgtagatg acgacggtga
     8281 cgtcctgccg ctcgcgcgcc atgctccaca gcgcctgcat cgtgtaggcc gcaccaccgt
     8341 ctccgtgcgg gcagacgacc ttgcggtccg ggcaggcgag ggcagcgccg agtgccagcg
     8401 gaagcccgag cccgatcgag ccaccggtga gcgcaaggtg atcgtgcggg cgggcacggg
     8461 ccatcatcat cgcgagcggc atgcctgatg tcgccgactc gtcggccatg atgacgtgct
     8521 cgggtgtcag gtgcgcgatc gcctgtgcgg ccgaaaccgc attgagggcc ccgcgcggca
     8581 ggtccggcag gcccagcggg acgcgcgcaa cgcccggtgc gggtccgcct agcgcggcat
     8641 cgagcgcctc gagggcctgc acgccgtcct cgtgctcctg cgcgagctgc gcgaagcggc
     8701 agccgtccgg cgcgcaccag ctgcgcttgc ccgggtaagc gaagaacgaa accggcggct
     8761 tcgatcccac gaggatcagc tgctcgacct ttgcgagcgc atccacgatc tgctccgcga
     8821 agtacggcag tcgctcgacc gggacgcggc ccgcgcccag ctcgctgtgc ggcgcgaagg
     8881 tatcggacag cagccgcgcg ccggtcttcg cggcgatgcg tcccgccgct tcgaggccgg
     8941 cgccgcgcag cgcgcgaccg cgaagcagga tggccgtgcg cttgccgttc ttcagcaact
     9001 gcgctgcctc gtcgaccgca gcggacgcga cgggctgcgg cgcgatcggc ggcagcgggc
     9061 ccgcggcatg gctcgcttcg gtccacgcgg tgtcggcagg caggatcagg gtggcgacgc
     9121 ccccaggcgc cgcacacgcg gcctgcacgg cgcgggccgc gtcggcggcg accgt
//
LOCUS       DAOMFD010000077         9174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2266_length_9174_cov_31.443908, whole genome shotgun sequence.
ACCESSION   DAOMFD010000077 DAOMFD010000000
VERSION     DAOMFD010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2266_length_9174_cov_31.443908"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(505..2241)
                     /gene="recJ"
                     /locus_tag="PL152_06080"
     CDS             complement(505..2241)
                     /gene="recJ"
                     /locus_tag="PL152_06080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HQR48883.1"
                     /translation="MSRRILRRAIDEQALPASATPLHPAVRRVYAARGVHCDEDLDLS
                     MERLLPVGSLGGVDAAVDLLLACHRTGGKVLVVGDFDADGATSTAVVVRQLRRLGFVD
                     PGFLVPDRFKYGYGLTPEIVRVAAQMRPALIVTVDNGISSLEGVAEARREGIPVLVTD
                     HHLPGAALPDAAAIVNPNAPGERFASKALAGVGVAFYVVAALTRRMRESGLVGKAADV
                     NAADLLDLVALGTVADVVPLDLNNRVLVAQGLRRIRAGRCAAGLRALLESAGRRLETV
                     IAQDLGFQAGPRLNAAGRLEDMSLGIECLLTAEAAEARELASRLAQLNSERREIEHRM
                     QQEALAKVDELVASLEGGMPAGLCLYDAGWHQGVIGLVASRVKERVHRPVIAFAPGDP
                     GWVKGSARSVPGVHVRDALDAVATRNPGLLEKFGGHAMAAGMTLRAARLAEFGQAFAD
                     EIARRIDEDTLTGDLHTDGPLLPGEFNADTAIALREAGPWGSGFPEPSFDGCFGVVTA
                     RIVGDRHLKLRLKAGSGEAVDSIAFRYLDDADAAPVRAGQDVEIVYRTALDEYGGARR
                     MQLVSEWLRTLP"
     gene            complement(2238..>2747)
                     /locus_tag="PL152_06085"
     CDS             complement(2238..>2747)
                     /locus_tag="PL152_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48884.1"
                     /translation="LRDLMPTGRDAAAIRSLTNEIQMLLHEHPVNVARASRGLPVINS
                     LWLWGFGELGEPIQLDLPLLFTDDAWLAGLWQLHGRSARGLSDLPAESWPPVGQALVA
                     WSRCAPGSPTEALATAERQCFVPLSSALRAGRVNRIEMLLGSCAFVVDRKARWRFWRR
                     GRSLQSVFE"
     assembly_gap    2748..2847
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3121..3501
                     /locus_tag="PL152_06090"
     CDS             3121..3501
                     /locus_tag="PL152_06090"
                     /inference="COORDINATES: protein motif:HMM:NF016326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HQR48885.1"
                     /translation="MKISLDVPAAFHVVRGYSPGALRIGERTFNRSLILTASTIVEGW
                     RPLSIAELAASDLEPLLELRPEVLLIGTGRRQQFPEHGVLARLYAARLGFEVMDTGAA
                     CRTYNLLVAEGRSVAAGLIVEPAA"
     gene            3596..5296
                     /locus_tag="PL152_06095"
     CDS             3596..5296
                     /locus_tag="PL152_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR48886.1"
                     /translation="MDRYWLKNYPGHVPAEVDVAQYASVTQLLEASFAKYPRHKAFAY
                     MDRDFTFADVDRYSAALGAFLQGLGLERSARVAIMMPNVIQYPICVAGILRGGYTVVN
                     VNPLYTPRELLHQLTDSGAEAIIVLDMFCATLEQVVAQTAIRHVLVATMGEMLGVPKG
                     IAVDFVARRVRKLVPPFSLPGHHRFKHALKGGARRGLRRVEQARTDLALLQYTGGTTG
                     LSKGAMITHGNMVANLAQASAWVGDAAGVTPEDPDADQPVGLCALPLYHALALVLVCL
                     FGMHTGRLSVLVANPRDLPGLMRVLRKYRFHVVNGIDTLLNAIAEHPDFRSIDFSRMR
                     IVGTGGMAGKRAVSDKWRTVTGLAVSQGYGMTEVTAAATMDPLPAREFTDSVGMPIPG
                     TEIAILDESDRPVPPGEPGEVAIRGPQVMAGYWRRPEETAKVMTPDGFLKSGDIGIMD
                     ERGFLRIVDRKKDMIIVSGFNVYPTEIEQVIATHPGVLECAAIGVPDDRSGEAVRLFV
                     VRKDPALTEQELIGYCRDNLTGYKIPRQVAFRESLPKSPVGKILRRELREAASAMPHA
                     "
     gene            complement(5354..>6145)
                     /locus_tag="PL152_06100"
     CDS             complement(5354..>6145)
                     /locus_tag="PL152_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOFRL family protein"
                     /protein_id="HQR48887.1"
                     /translation="RRHLSGIKGGRLAAACYPANVVTLAISDVPGDDPVDIASGPTVA
                     DPTTCADALAVLDRYRIDLPRHVRAGLERGAWESVKPDSPRMPEAAFRLVAAPQQALV
                     AAAVVATAHGVTPLILSDRIEGEARDVGRDMANLAAATARGEGPVRPPCVLLSGGETT
                     VTVQGNGRGGRNVEFLLSFGCELGPASRIHALACDTDGVDGMEPVAGAIWHPDSLLRA
                     ASLGLDAHAYLDANDAHSFFAALGDSVVTGPTRTNVNDFRAILVR"
     assembly_gap    6146..6245
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6246..6868)
                     /locus_tag="PL152_06105"
     CDS             complement(<6246..6868)
                     /locus_tag="PL152_06105"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019752402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HQR48888.1"
                     /translation="MTRHGMTGIGMTSARTRDRLIARLREQGIANPHVLERIRAVPRH
                     LFVDEALASRAYEDTALPIGHGQTISQPYIVARMTEALLEDAAPRKVLEVGTGCGYQT
                     AVLAPLVTTLYSVERIAALQQRARRILRDLKITNVFMRHGDGFEGWPPYAPYDGIIVA
                     AAAMAVPKALLDQLGNGGRLIMPVGPDREQVLLRITRRGENFERESLG"
     gene            complement(6882..7634)
                     /gene="surE"
                     /locus_tag="PL152_06110"
     CDS             complement(6882..7634)
                     /gene="surE"
                     /locus_tag="PL152_06110"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003231188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HQR48889.1"
                     /translation="MRILVSNDDGYRAEGLRRLSEALRPLAEVTVVAPDRNRSGASNS
                     LTLDVPVRVFKFGERMYYVNGTPTDCVHLAISGLFDFEHDMVVSGINDGANLGDDVLY
                     SGTVAAAIEGRFLGLPTMAVSLVARDGRNFDTAARVATELVMRLLRSPLDASMILNVN
                     VPDVPYEQLKGMRVTRLGHRHKSEPVVRAADPRGRPVYWVGPAGEGADAGEGTDFHAI
                     AASCVSITPLQVDLTRHTALAEVDEWLKGRGP"
     assembly_gap    7864..7963
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7964..8457)
                     /gene="truD"
                     /locus_tag="PL152_06115"
     CDS             complement(<7964..8457)
                     /gene="truD"
                     /locus_tag="PL152_06115"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: protein motif:HMM:NF013322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HQR48890.1"
                     /translation="MEFPTALGPPPLAGRTRVEPEDFFVEELLGFEPDGRGGYVLVTV
                     EKRGANTGWVAAELARAAGVAVSAVGYSGQKDRHALTRQAFSLPWPAAAPVEPCMSFS
                     AEGFRVLAVARHGRKLRPGSHESNRFVIRIREAAGDTAVIEARLAEIGRRGVPNYFGP
                     QRYGR"
     gene            complement(8462..8953)
                     /gene="ispF"
                     /locus_tag="PL152_06120"
     CDS             complement(8462..8953)
                     /gene="ispF"
                     /locus_tag="PL152_06120"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HQR48891.1"
                     /translation="MRVGIGYDVHAYGPGDHVMLGGVRIEHERGVQAHSDGDVVLHAL
                     VDALLGAMGLGDIGQHFPDTDPRWKGADSRRFLRHCAVLLGRHRWRLVNADITVVAEA
                     PRLGAHRPEMRARIAEDLECDAARINIKATTHEGLGALGRGEGLAAQAIVLLAHADAP
                     HHP"
     gene            complement(8950..>9174)
                     /locus_tag="PL152_06125"
     CDS             complement(8950..>9174)
                     /locus_tag="PL152_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013308.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HQR48892.1"
                     /translation="LWRALTPQMFRYGLLRRALALCLERERSVTDEASAIEALGLKPR
                     LVPGSADNIKLTAPADRALAEAILRMGDRR"
BASE COUNT         1387 a         3203 c         2975 g         1309 t
ORIGIN      
        1 gcccagcgca ggtacatgcg catgagcatc tgcgcccagt cctgggcctc ggtgccgccg
       61 gcgcccgcct ggatgtcgag gaagcaattg tgcgggtcca tctcgccggg gaacatgcgc
      121 tggaactcga gcttcgcgat ctcgccttcc aggcggccca cgtcctgctc gaccgacttg
      181 acggtgccct cgtcggcctc ggcctctgcc agctcgagca gttccccggc ttccgtgatc
      241 ccgccgctca gccggtcgag cgtcctgacg atcgattcga gcttggtgcg ctcgcggccg
      301 agttcctgcg cccgctccgg cttctgccag acggcgggat cctcgagttc cctcaggacc
      361 tcggtgaggc gctcgctgcg accctcgtag tcaaagatac cccctgagcg cgttggagcg
      421 ctgcgcgaga tcgtcgaggg aacggcggat cgggttggtt tccatcagac gctgcacgga
      481 tgtcgagttc aaacgagaat tctatcacgg cagcgtccgc agccattcgc tgacgagctg
      541 catgcgccgt gcgccgccgt actcgtcgag cgccgtgcgg tagacgatct cgacgtcctg
      601 cccggcacgg accggcgcgg cgtccgcatc gtcgaggtac cggaacgcga tcgagtcgac
      661 cgcttcgccg gaaccggcct tcaggcgcag cttgaggtgc cggtcgccga cgatccgcgc
      721 ggtgacgacc ccgaagcacc catcgaacga cggttcggga aatcccgatc cccacggccc
      781 tgcctcgcgc agggcgattg ccgtatcggc attgaactcg cccggcaaca gcggcccgtc
      841 ggtgtgcagg tcgcccgtga gggtgtcctc gtcgatgcgc cgggcaatct cgtcggcgaa
      901 cgcctgcccg aactcggcca ggcgcgctgc gcgcaacgtc atgcccgccg ccatggcgtg
      961 gccaccgaac ttctcgagca ggccgggatt gcgcgtcgcg acggcgtcga gcgcgtcgcg
     1021 gacatgcacg cccggcaccg aacgggccga ccccttgacc cacccgggat caccgggtgc
     1081 aaaggcgatc acgggccggt gcacccgctc cttgacccgc gacgccacga gaccgatcac
     1141 gccctggtgc catccggcgt cgtagaggca cagtcccgcg ggcatcccgc cctcgagcga
     1201 ggccacgagt tcgtcgacct tcgcgagcgc ctcctgctgc atgcggtgct cgatctcgcg
     1261 ccgttcgctg ttcaattgag cgagtcgcga tgccaactcg cgagcctcgg ctgcctcggc
     1321 agtgagcagg cactcgatgc cgagcgacat gtcctcgaga cgacccgccg cattcagccg
     1381 tgggcccgcc tggaacccga ggtcctgcgc aatgactgtc tcgagccggc gtcccgccga
     1441 ttcgaggagc gcacgcaggc cggccgcgca gcgtccggcg cggatccgtc gcaggccctg
     1501 ggccaccagg acgcgattgt tgaggtcgag cggcacgacg tcggccaccg tgccgagcgc
     1561 gaccaggtcg agcagatcgg ctgcgttgac gtcggccgcc ttcccgacga gccccgactc
     1621 gcgcatgcga cgcgtcagcg cagccacgac gtagaacgcg acgccgacgc ccgcgagcgc
     1681 cttggaagcg aagcgctccc ccggtgcatt cgggttcacg atggcggcag cgtcgggcaa
     1741 ggcagcgcct ggaaggtgat gatcggtgac gagaaccggg atcccctcgc ggcgcgcctc
     1801 ggcgacgccc tcgagcgacg aaatgccgtt gtcgaccgtc acgatcagtg ccggccgcat
     1861 ctgcgccgca accctgacga tttccggcgt gagcccgtat ccgtacttga accggtcggg
     1921 aacgaggaat cccgggtcga cgaaacccag tcgccggagt tgtcggacca cgaccgcggt
     1981 gctggtcgca ccgtccgcat cgaagtcacc gacgaccagc accttgccgc cggtgcgatg
     2041 gcaggcgagc agcaggtcca cggctgcgtc cacgccgccc aacgagccga ccggcagcag
     2101 tcgctccatg ctgaggtcga ggtcctcgtc gcaatggaca ccgcgcgccg catacacgcg
     2161 gcgcacggcc ggatgcagcg gcgtcgccga cgcgggcagc gcctgctcgt cgatcgcgcg
     2221 gcgcaggatg cggcggctca ttcgaacacc gactgcagcg accggccgcg ccgccagaac
     2281 cgccagcggg ccttgcgatc caccacgaac gcgcagctgc cgagcagcat ttcgatccgg
     2341 ttcacccggc ctgcgcgcag ggccgaagac aagggaacaa agcactgccg ttccgccgtc
     2401 gccagcgctt ccgtcgggga tccgggcgcg caccgtgacc acgcgacgag tgcctgtcca
     2461 acgggcggcc aggattccgc cgggaggtca ctcaggcccc gtgccgaacg cccgtgcagt
     2521 tgccacagtc cggcgagcca cgcgtcgtcc gtgaacagca gcgggagatc cagctggatc
     2581 ggctcgccca gctctccgaa gccccacagc cacagtgaat tgatgaccgg gagcccgcgc
     2641 gatgcgcgtg cgacgttgac tggatgctcg tgcagcagca tctggatctc gttcgtgagc
     2701 gaccgtatgg ccgccgcatc ccgtccggtc ggcatcaggt cgcgcagnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnn nnnnnnncgc tcgagcagtc ctgcggggag tcccgccccg
     2881 tcgaggagcc actcccgcca gctgctgacc gggccgccca cgcggtcggc gcgtgcaagc
     2941 agccactcgc ccgccggcga gcgcggaagc cgcgtgtcct ccgggtcccc gggaaaaagg
     3001 ccaggcaacc acggaattgc gagtcgcaaa cgaaccgacc gactttcagc caagccgcca
     3061 cctctccatg gaatcctgcg accggtgacc cgcgccgccc tcgtccggta tgttaaccgc
     3121 atgaagatca gtctcgacgt gccagcggcg ttccatgtcg tgcgcgggta ctcacccggc
     3181 gcgctacgca tcggcgagcg gacgttcaat cgcagcctga tcctgacggc gtccacgatc
     3241 gtcgaaggct ggcgaccgct ttccatcgcg gaactcgcgg cgtccgacct ggaaccgctg
     3301 ctcgagctgc gtcccgaagt gctgctgatc ggcaccggac ggcgccagca gttcccggag
     3361 cacggagtcc tcgcccgcct gtacgcggca cggctcggtt tcgaggtcat ggacacgggc
     3421 gccgcctgcc gcacgtacaa tctgctcgtg gccgaggggc gcagcgtcgc cgccggactg
     3481 atcgtcgagc cggcggcgtg acacccggcg gaccggttgc agccatggca agaccgcccg
     3541 ccgatgggtc ggtgctcacc gctctgatca ccccgcaacc aggggaacac gcgcgatgga
     3601 ccgctactgg ctgaagaact atccgggcca cgtgcctgcc gaggtcgacg tcgcgcagta
     3661 tgcgtcggtc acgcagctcc tcgaggcgtc cttcgcgaaa tacccgcgcc acaaggcgtt
     3721 cgcgtacatg gaccgcgact tcaccttcgc ggacgtcgat cggtactccg cggcgctcgg
     3781 cgcgttcctc cagggcctgg gcctggaacg ttcggcgcgc gtggcgatca tgatgccgaa
     3841 cgtcatccag tacccgatct gcgtcgccgg gatccttcgc ggtggataca cggtggtcaa
     3901 cgtcaacccc ctctacacgc cgcgggaact gctgcaccag ctgacggact cgggggcgga
     3961 ggccatcatc gtcctcgaca tgttctgcgc aaccctggag caggtcgtgg cgcagaccgc
     4021 catccggcac gtcctggtcg caacgatggg cgagatgctc ggcgtcccca agggcatcgc
     4081 cgtcgacttc gtggcgcgcc gggtcaggaa gctggtgccg cccttttcgc tgcccgggca
     4141 tcaccggttc aagcacgcac tgaagggcgg cgcgcgacgc gggctgcggc gcgtcgagca
     4201 ggcgcgcacc gatctcgcgt tgctgcagta caccggcggg accaccggcc tgtcgaaggg
     4261 cgcgatgatc acgcacggca acatggttgc caatctcgcc caggccagcg cctgggtcgg
     4321 ggatgccgcg ggcgtgaccc ctgaggatcc cgatgcagat cagccggtcg gcctctgcgc
     4381 gctgccgctc taccacgccc tcgcgctcgt gctggtctgc ctcttcggga tgcacacagg
     4441 caggctctcc gtgctggtcg caaatccgcg cgatctgccg gggctcatga gggtgctgcg
     4501 caagtaccgc ttccatgtcg tcaacggcat cgacacgctc ctcaacgcca tcgcggagca
     4561 cccggatttc cgcagcatcg acttctcgcg catgagaatc gtgggtaccg gtggcatggc
     4621 cggcaagcgg gcggtctcgg acaagtggcg cacggtcacg ggactggccg tgagccaggg
     4681 atacggcatg accgaggtca cggctgccgc gaccatggac ccgctgccgg cccgcgaatt
     4741 cacggacagc gtcgggatgc cgatacccgg cactgaaatc gcgatcctgg acgagtctga
     4801 ccgcccggtc ccgcccggcg agccgggtga ggtcgccatc cgcggtccgc aggtgatggc
     4861 aggttactgg cgcaggcccg aggagaccgc gaaggtcatg acgcccgacg gattcctgaa
     4921 gtcgggcgac atcgggatca tggacgaacg gggtttcctg cgtatcgtgg accgcaagaa
     4981 ggacatgatc atcgtgtcgg gattcaacgt gtacccgacc gagatcgagc aggtcatcgc
     5041 cacgcacccg ggcgtgctcg agtgcgcggc catcggagtt cccgacgatc gatccgggga
     5101 ggccgtgaga ctgttcgtgg tccgcaagga tccggcgctt acggagcagg aactcatcgg
     5161 ctactgccgc gacaatctga ccggatacaa gattccccga caggtggcat tccgggagtc
     5221 cctgccgaag agcccggtcg gcaagatact gcgacgcgag ctgcgcgagg cagcgagcgc
     5281 gatgccgcac gcttaggccc aactgcggac gcagcgcggg cgacgagcgg accagccacg
     5341 aaagtccatg ccttcaacgc accaggatcg ccctgaaatc gttcacgttc gtgcgcgtcg
     5401 ggcccgtcac gaccgagtcc cccagcgcgg cgaagaacga gtgcgcgtcg ttggcgtcga
     5461 gatacgcgtg ggcatcgagg ccgaggctcg ccgcgcgcag cagcgagtcc gggtgccaga
     5521 tggcccctgc gaccggctcc attccgtcca cgccgtccgt gtcgcaggcg agcgcgtgaa
     5581 tccggctcgc ggggccgagc tcacacccga acgacagcag gaactcgacg ttgcgcccgc
     5641 cccgcccgtt gccctgcacg gtgacggtcg tctcgccacc cgacagcagc acgcacggtg
     5701 gacgcaccgg cccctcgccc ctggcggttg cggcggcgag attcgccatg tcgcggccga
     5761 cgtcgcgtgc ctcgccctcg atgcggtccg acaggatcag cggcgtaacg ccgtgcgcag
     5821 tggcgaccac cgcagcggct acgagagcct gctgtggcgc agcgacgagc cggaatgcgg
     5881 cttcaggcat tcgtggggag tctggcttga ccgactccca tgcgccgcgt tcgaggccgg
     5941 cgcgcacgtg tcgcgggagg tcgatgcggt atcggtcgag cacggcgagc gcgtcggcac
     6001 aggtcgtcgg gtcggcgacg gtcggccccg atgcgatgtc cacgggatcg tcgcccggca
     6061 cgtccgagat ggcgagcgtc acgacgttcg ccgggtaaca ggctgcggcg agccgcccgc
     6121 ccttgatgcc cgacaggtgc cgacgnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnccgag tgactcccgc tcgaagttct cgccccgccg cgtgatgcgg agcagcacct
     6301 gctcgcggtc cggcccgacc ggcatgatga gccgcccgcc gttgccgagc tggtcgagca
     6361 gcgccttcgg gaccgccatg gccgccgctg cgacgatgat gccgtcgtac ggcgcgtacg
     6421 gcggccagcc ctcgaagccg tcgccatgcc gcatgaacac gttggtgatc ttgaggtcgc
     6481 ggaggatgcg ccgcgcgcgc tgctgcaggg cggcgatccg ctcgacgctg tagagcgtcg
     6541 tcaccagcgg cgcgagcacc gccgtctggt agccacaccc ggtgccgacc tccagcacct
     6601 tgcgcggcgc cgcgtcctcg agcagtgcct cggtcatgcg cgccacgatg tacggctgcg
     6661 aaatcgtctg cccgtgcccg atcggcagtg cggtgtcctc gtaggcgcgg ctggcgaggg
     6721 cctcgtccac gaacaagtgc cggggcacgg cgcgaatgcg ctcgagcacg tgcgggttgg
     6781 cgatgccctg ctcgcgcagg cgcgcaatca gccggtcgcg ggtgcgcgcc gacgtcatgc
     6841 cgatgccggt catcccgtgc ctggtcatcg cccgccccgc gtcacggccc ccgccccttg
     6901 agccactcgt cgacctcggc gagcgccgtg tggcgcgtca ggtccacttg aagtggagtt
     6961 atcgatacgc agctggccgc aatcgcatgg aagtcggtgc cctcgcccgc gtctgcacct
     7021 tcgccggccg ggccgaccca gtacacgggg cggccgcgtg gatcggcagc ccgcaccacc
     7081 ggctcggact tgtggcggtg acccagccgc gtcaccctca tgcccttcag ttgctcgtac
     7141 ggcacgtcgg gcacgttcac gttgaggatc atcgacgcgt cgagcgggct gcgcagcagg
     7201 cgcatcacca gctcggtcgc cacgcgcgcc gccgtgtcga aattccggcc gtcacgcgca
     7261 acgagcgaca ccgccatggt cggcaacccc aggaaccgcc cctcgatcgc cgccgcgacc
     7321 gtgccggagt acagcacgtc gtcgccgaga ttggcgccgt cgttgatgcc ggacacgacc
     7381 atgtcgtgct cgaagtcgaa gagcccggag atcgcgaggt gcacacagtc ggtgggcgta
     7441 ccgttcacgt agtacatgcg ctcgccgaac ttgaacacgc gcaccggcac gtccagggtg
     7501 agggagttgc tcgcgccgct gcgattgcgg tcgggcgcca cgaccgtcac ttccgcgagc
     7561 ggccgcagcg cctcgctcag ccggcgcagg ccctcggccc gatagccgtc gtcgttactg
     7621 accaggattc tcaagccggg agcccacgca atgttcgcgg aactatactg gaatcgcccg
     7681 gcgacgcacg cccgaggcta ctcatcgccg ccctcgctcg agtcccacgc gtcctgcagg
     7741 agctcgtgca gcaccgtcgt ggcgaacgtc ccgcgcggca ggacgaacga gacgaccagc
     7801 gcatcgtccg cgcgggtcca ttcgagcgag cgcaccggca gccgcaggct gcgccgctcg
     7861 tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnncgcccgt agcgctgcgg
     7981 tccgaagtaa ttcggcacgc cccgtcgccc gatctcggcg aggcgcgctt cgatcacggc
     8041 ggtgtcgccc gcggcctccc ggatccggat cacgaagcgg ttggactcgt gcgaccccgg
     8101 ccgcagcttg cgcccgtgcc gggcgacggc aagcactcga aagccttccg cggagaacga
     8161 catgcacggc tccacgggtg ccgctgctgg ccacggcaac gagaacgcct gccgcgtgag
     8221 tgcgtgccgg tccttctgcc cgctgtagcc gaccgcgctg accgccacgc ccgccgcgcg
     8281 cgcaagctcg gcggcaaccc aaccggtgtt cgccccgcgc ttctcgaccg tgacgaggac
     8341 gtatccgccc cggccgtccg gctcgaagcc aagcagctcc tcgacgaaga aatcctccgg
     8401 ttcgacgcgc gtcctcccgg ccagcggagg cgggccgagt gcagtgggaa actccatgag
     8461 ctcagggatg atgcggagcg tcggcgtgcg cgagcagcac gatcgcctgc gccgcgagtc
     8521 cctcgccgcg gcccagcgca cccagcccct cgtgcgtcgt cgccttgatg ttgatgcgcg
     8581 cggcgtcgca ctcgaggtcc tcggcgatcc gggcccgcat ctcgggccgg tgtgcgccga
     8641 gacgcggcgc ctcggcgacg accgtgatgt cggcattcac gagccgccac cggtgccttc
     8701 ccagcagcac cgcgcaatga cgcaggaagc gccggctgtc ggcgcccttc cagcgcgggt
     8761 cggtatccgg gaagtgctgc ccgatgtcgc cgagccccat ggctccaagc aacgcgtcga
     8821 ccagcgcatg cagcaccacg tcgccgtcgg agtgcgcctg gacgccccgc tcgtgctcga
     8881 tgcgcacgcc accgagcatc acgtggtcgc ccgggccgta cgcgtgcacg tcgtagccga
     8941 ttcccaccct catcgcctgt cccccatccg caggatcgcc tcggccagcg cacgatccgc
     9001 cggggccgtc agcttgatgt tgtccgcgct gccgggcacg agccgcggct tcagtcccag
     9061 cgcctcgatg gccgaggcct cgtcggtgac gctgcgctcg cgctccaggc agagcgccag
     9121 cgcacggcgc agcaacccgt agcggaacat ctgcggcgtg agcgcgcgcc acag
//
LOCUS       DAOMFD010000078         9022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2337_length_9022_cov_30.233969, whole genome shotgun sequence.
ACCESSION   DAOMFD010000078 DAOMFD010000000
VERSION     DAOMFD010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 9022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9022
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2337_length_9022_cov_30.233969"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..871
                     /locus_tag="PL152_06130"
     CDS             110..871
                     /locus_tag="PL152_06130"
                     /inference="COORDINATES: protein motif:HMM:NF025433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HQR48893.1"
                     /translation="MQMQTQSHDAPEAGATPAGSRRRRRRRAILVGLVVWAIAAYLVA
                     PRLWTWYLERTKPLPDVARVTHTSDGHPGDPVNIALEGSKEEVIKAMTAAGWYPADPV
                     TLSSSVRIAVDTALRRADDMAPVSPLYLFGRVQDLAFEQPVPGGPRHRHHVRYWLWDQ
                     QRDGSPVWFGAVTYDERAGLSYTTGEVTHHIGSDVDAERDRVVAELTNAGWVGGVQWI
                     DDFHRQLEGRNGGGDLWRTDGRLAVARLAARPSVR"
     gene            complement(946..1413)
                     /locus_tag="PL152_06135"
     CDS             complement(946..1413)
                     /locus_tag="PL152_06135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR48894.1"
                     /translation="MKAFHCIALALVLLSPAGPALAASTSASAIPKAALVQPAELAAT
                     LRNASAPKPLLLHVGFHVLYQQARIPGSEYVGPASEQAGLQLLRDRVAKLPRNTPIVV
                     YCGCCPWGDCPNIAAAYEALRALGFTNVKALYIAHNFGTDWMDQGHPVSTRGS"
     gene            complement(1469..2332)
                     /locus_tag="PL152_06140"
     CDS             complement(1469..2332)
                     /locus_tag="PL152_06140"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051213 - dioxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQR48895.1"
                     /translation="MSLDIRRLGPSLGAEIRGVDLAQPVDADTVGEIARAFDEHGVLV
                     FRGQAIDDRAHARFAAHFGPLGTFRHLADPHGKVREIFRLANTDAAGNLLPADDAHLR
                     LLELNGLWHSDSSYRQVPTRGSVLRAVVVPDSGGDTLFADMAAAYETLPATMRRRIAG
                     LYARHSLEFLVTSRGLPALGAEELARIPPVEHPLVREHASGRCSLFLSPPYMEAIVGW
                     SPAASRALIAELTDWATRERYTYRHHWQRDDLVMWDNGWTMHKVLPYDIARCRRVMHG
                     TTLLGGSAHAA"
     gene            complement(2359..3237)
                     /locus_tag="PL152_06145"
     CDS             complement(2359..3237)
                     /locus_tag="PL152_06145"
                     /inference="COORDINATES: protein motif:HMM:NF017532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HQR48896.1"
                     /translation="MAGRSFGASVVNLARRDIDELRTAFAGDGVACIRGALDATALEL
                     AERTFQWSLDHPGPSARQVLAGRPGAFYQDHANPDALPMHLALLRDTGLAELAALVLG
                     SESLWLLYEQIWLKQGGDTLRTPWHQDLPYIPLEGNHVATMWVPLDPVRRECALEFVR
                     ASHRGPLFNPTAFDPDDPAAAMYADGVWPPLPDVESDRAAWPIVAWAVEPGDVILFHM
                     AMLHGGAPTRAGERRRTISLRFFGDHAYCAGRPESGLSAEDRLRRDDGGRDPIEQMAY
                     APPGSLFRHPGFARLR"
     gene            complement(3204..5831)
                     /locus_tag="PL152_06150"
     CDS             complement(3204..5831)
                     /locus_tag="PL152_06150"
                     /inference="COORDINATES: protein motif:HMM:NF017000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase LanC family protein"
                     /protein_id="HQR48897.1"
                     /translation="MLDGIDPLAAAESYSAALRRLSERLARDWVATDWSDGPLSWRFF
                     APREEPTPAQGWKIHVSASAAESASLLPVVGAVARAQRAAFKVPRSIDDVVFLNSGDA
                     GVQQLGKIACVYPRDDAHARELLDRLDAAWPASNGPEVQTDLHVRPGSAVSFRYGAYG
                     GGGTVASSAGLHAFAIVLPDGSTTADTRRDDGAQLPGAPPPPIAGCPPRPCPIAVGDT
                     LQVATRRITVLAQLSDSPRARTFLGADLDSLETVVLKTARPGVAGDATGLDVRDRLAR
                     EFDVLTALAARAGVAPCALAWQDGEWPLLVMEDFRGESLAELPRRQRIERLPQLADAV
                     ARVHAAGFVHGDVKLANAVCRGDGVGLIDFELAEPVGTIARSGGTRGHLAPETVDRVP
                     MTPARDVYALGACVAHAVLDTPPALLGTGSHGLGALLRNEGAHSAARLVETLTARDPD
                     IRPTASAAAAALSSCVDSLAEFVPSTGHPGTEPERGWYRHACNDSARLAVTYATTTRE
                     GTHWRNTHFMRAFDCEAINLGAAGIVLGLLTVDTASHRAGFADAVMGGARWLAARPAE
                     GNAAGLFTGNAGVAIALAVTGRRFGDESLLAAARERFTVACADRRELDLFSGAAGVAW
                     TACLLSDVLDERWPLEVAGGAVDGIRARSFESDGIPIWTTDPASDPAYLGCAHGSSGI
                     AMALACWGRRRRDQECVDTAREAFRRIARAGRTSDGLALRMRANGTGRHAVGNWCHGV
                     AGWLWCVLQGLGDDDTLRAEIDWAVDSLRDATSTGTPTYCHGLAGQLELWRMLRGVPR
                     HGALADARAGKVARALRIVHHERDGHCAWISDDPDVVTPDLWIGFLGPATALALHAIG
                     SNAPLLSAEWLAAVSARAS"
     gene            complement(5815..6036)
                     /locus_tag="PL152_06155"
     CDS             complement(5815..6036)
                     /locus_tag="PL152_06155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48898.1"
                     /translation="MNVSADALRAKVDHLRVAVHAGAVSPVDAIVELKDAAAPPVTEA
                     DGHGGTLHMCPWHTCQATRITPDAVAGRH"
     gene            complement(6197..7270)
                     /locus_tag="PL152_06160"
     CDS             complement(6197..7270)
                     /locus_tag="PL152_06160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48899.1"
                     /translation="MIHPPPLAGRPKDGPQLWLSPEYFPIRLAPEANALTLVPMSEQL
                     YREVNFLDDREIPMDGSREVPLDAAIRALDQLHPPPRPLHWIFHVAFCGSTLVSRCLE
                     QLATAFVLKEPFAVHDLAHRRRHGPADPAGPGGWHDSLRLLDALLARTYRSDQVAIVK
                     ATDASTQLLGDVLALNPGSKALFQYVGVEEFLLAMLGDRVRMGFVRERLNDLAVLFPD
                     EFAFRREAWQSLPDASQAACLWVLHLRLYARFAGSTHGGRCRSLDFARFLRDPQESLT
                     ALARFLGLGCSSRDVEAAIATTLAVHSKAPRAAFSTADRAARLKSAAEEHRDAIDASL
                     AWACRRFPDFAVLQAPPNALPAT"
     gene            7388..8593
                     /locus_tag="PL152_06165"
     CDS             7388..8593
                     /locus_tag="PL152_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HQR48900.1"
                     /translation="MRATGPSTTGDRIDLPVLDPAFKADPHSRYAQWRAQGRTIVPIR
                     LPTGLEAWLVIGYDAARELLNDPRLSKSDPGGSASAAHRLFRHMLTLDPPEHTRLRSI
                     IAREFTVRRVALLRPRIREIVVRLIDEASRAETADLMQALALPLPLQVICELIGMPAG
                     DDALVLRWSRRLAAADLDEPADVPGIADEIHGHLARLAEGKRRAADDSLLSGLVAAQD
                     RGELSGTELTSMTFLLLSAGHETTANLIGNGVLALLRNPAQWSALCADGDHARAIVEE
                     VLRFDSPLEVSTIRHATSEIELGDVRIRPGDTVFIGLAAANRDPRRIERAGEFDPGRH
                     PAAAHLAFGHGVHFCVGATLARAEGETAFVELSRRFPRLALDVPLDALEWTPGLIMRG
                     PLRLPVRFN"
     gene            complement(8586..>9022)
                     /locus_tag="PL152_06170"
     CDS             complement(8586..>9022)
                     /locus_tag="PL152_06170"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48901.1"
                     /translation="GSLGFTATAPAEGAVMTRTEVQERLRTILAGRAAEEVIFGKEDV
                     SLSAGGGDGSDLAVATRVATRVICTSGFSGDHSLLWTSSPTAAQLEQVEGLLRVCYRA
                     AIDLLRDQRAALDRIVQGLVERQELDGAAVRSLLDGRSAAAN"
BASE COUNT         1391 a         3159 c         3212 g         1260 t
ORIGIN      
        1 gtcgcgtcgt cgcgcggttc cgcggcgcgc gcgactggga cagcgccgag tcggtccggc
       61 tgatcgagca gtcgttcgcg gccggtcacc accccgggta ggtgacacca tgcagatgca
      121 gacccagtcc cacgacgcac ccgaagccgg tgcgacaccg gctggatcca ggcgccgccg
      181 tcgccggcga gccatcctgg tcgggctggt cgtgtgggcc atcgccgcat acctggtcgc
      241 gccgcgcctg tggacctggt atctcgagcg cacgaagccg ctgcccgacg tcgcgcgcgt
      301 gacgcatacg tccgacggcc acccgggcga cccggtcaac atcgccctgg agggcagcaa
      361 ggaggaggtc atcaaggcga tgaccgcggc gggctggtac cccgcagacc cggtgacgct
      421 gtcgtcgagc gtcaggatcg ccgtcgacac tgcgttgcgg cgcgccgacg acatggcacc
      481 cgtgagtccg ctgtacctct tcggacgcgt gcaggacctg gcattcgagc agcccgtgcc
      541 cgggggtccg cgacaccgtc accacgtgcg ttactggctt tgggaccagc agcgcgatgg
      601 ctcgccggtg tggttcggcg cggtgacgta cgacgagcgc gccggcctga gctacacgac
      661 cggcgaggtc acgcatcaca tcggctcgga cgtggacgcc gagcgggacc gggtcgttgc
      721 cgaattgacg aacgccggct gggtcggcgg tgtccagtgg atcgacgact tccaccggca
      781 actcgaaggg cgcaatggcg gcggggacct gtggcggacc gatggccgac tggcggtcgc
      841 gcggctggcg gcacgaccct ccgtcaggta ggcactcgac gaccgcatgc caccgggccg
      901 cccggtcccg ggacgacggt cagccgcggc acgcgaccac ggccgtcatg atccccgtgt
      961 ggaaacgggg tggccctggt ccatccagtc cgtaccgaag ttgtgcgcga tgtagagcgc
     1021 cttcacattg gtgaatccca atgcgcggag cgcctcgtac gcggcggcga tgttcgggca
     1081 gtcgccccag gggcagcagc cgcaatagac cacgatcggc gtattccttg gcagcttggc
     1141 aacccgatcc cgcagcaact ggagcccggc ctgctcgctg gccgggccca cgtattcgga
     1201 tcctggaatg cgcgcctgct ggtagagcac gtggaaaccg acgtgcagca gcagcggctt
     1261 cggtgcggaa gcgttcctca gggtcgctgc gagttccgca ggctgcacca gcgccgcctt
     1321 ggggatcgcc gacgcgctgg ttgacgcggc cagcgcaggc ccggccggtg acagcaggac
     1381 gagcgcaaga gcgatgcaat ggaatgcttt catcgaagtg tgcctcacgg tggccgccgc
     1441 attcgatgcg gccggcgtcg gggtcaccct acgccgcgtg agcgctgccg ccgagcagcg
     1501 tcgtgccgtg catgacgcgt cggcaccgag cgatgtcgta cggcagcacc ttgtgcatgg
     1561 tccagccgtt gtcccacatc accaagtcgt cgcgctgcca gtggtggcga taggtgtagc
     1621 gctcccgggt cgcccagtcg gtgagttccg cgatcagcgc gcgactcgcc gccgggctcc
     1681 agccgacgat cgcctccatg tagggagggc tcaggaagag ggaacatcga ccactcgcgt
     1741 gctctcgcac cagcgggtgc tccaccggcg ggatcctcgc gagttcctcg gcgccgagcg
     1801 cgggcagtcc gcggctcgtg acgaggaact cgaggctgtg gcgcgcgtag agtcccgcga
     1861 tccgccgtcg catcgtcgcg ggcagcgtct cgtacgccgc ggccatgtcg gcgaacaacg
     1921 tgtcgccgcc cgaatcgggc acgacgacgg cgcgcaggac cgagcctcgc gtcggaacct
     1981 ggcgatacga gctgtccgag tgccacaggc cattcagctc gagcaggcgc aggtgcgcgt
     2041 cgtcggcggg cagcaggttg ccggccgcat ccgtgttcgc gaggcgaaag atctcgcgga
     2101 ccttaccgtg cggatcggcg aggtgacgga acgtgcccaa cggcccgaaa tgcgcggcga
     2161 accgggcatg cgcgcggtcg tcgatggcct ggccacggaa gacgagcacg ccgtgttcgt
     2221 cgaatgcgcg tgcgatctcg ccgaccgtgt cggcatcgac gggctgggcc aggtcgacgc
     2281 cgcggatctc ggcgccgagg gacggtccca gcctgcggat atccagcgac atgcctcggg
     2341 tgctcacgac gtgacccgtc accgcaggcg cgcgaagcct gggtggcgga acagggagcc
     2401 cggcggtgcg tacgccatct gctcgatcgg atcgcgaccg ccgtcgtcgc gccgcaggcg
     2461 atcctctgcg gagagcccgg actcgggtcg gccggcgcag tacgcgtggt cgccgaagaa
     2521 gcgcagcgaa atggtgcggc gccgttcgcc ggcgcgcgtc ggagctcctc cgtgcagcat
     2581 ggccatgtgg aacaggatca cgtcgccggg ctcgacggcc caggcgacga tcggccaggc
     2641 ggcgcggtcc gattcgacat ccggaagtgg cggccacacg ccgtccgcgt acatcgccgc
     2701 ggccggatcg tccgggtcga aggccgttgg attgaacagc gggccgcgat gcgacgcgcg
     2761 gacgaactcg agcgcgcact cccgacgcac cgggtcgagc gggacccaca tcgtcgcgac
     2821 gtggttcccc tcgagcggaa tgtacggcag gtcctggtgc cagggcgtgc gcaacgtgtc
     2881 gccaccctgc ttgagccaga tctgctcgta cagcagccac aggctttcgc tgccgaggac
     2941 gagtgcggcc agctccgcga ggccggtgtc ccgcagcaac gcgagatgca tgggcaacgc
     3001 atcggggttg gcgtggtcct ggtagaaggc gcccggacgg ccggcgagca cctgtcgcgc
     3061 ggatggaccg ggatgatcga gcgaccactg gaaagtgcgt tcggccagct ccagcgccgt
     3121 cgcatcgagt gcgccgcgga tgcaggcgac gccgtccccg gcgaatgccg tgcgcagctc
     3181 gtcgatgtcc cggcgcgcga gattcacgac gctcgcgccg aaactgcggc cagccactcg
     3241 gcggacagca gcggggcgtt gctgccgatc gcgtgcaggg ccagcgcggt cgccggcccg
     3301 aggaagccta tccacaggtc gggcgtgacg acgtccggat cgtcggagat ccacgcgcag
     3361 tgaccgtcgc gctcgtgatg cacgatgcga agcgcgcggg cgactttgcc ggcgcgcgcg
     3421 tcggcgagcg caccgtgacg cggcactcca cgcagcatgc gccacaattc gagttgtccg
     3481 gcgagcccgt ggcagtaggt cggcgtgccg gtcgacgtcg catcgcgcag gctgtccacc
     3541 gcccagtcga tctcggctcg aagcgtgtcg tcgtcgccga gcccctgcag cacgcaccac
     3601 agccagcccg cgacgccgtg gcaccagttg ccgacggcat gacgacccgt gccgttcgcc
     3661 ctcatgcgca gcgcgagccc gtcgctcgtg cgacccgcgc gtgcgatgcg ccggaacgcc
     3721 tcgcgcgcgg tgtcgacgca ttcctggtcg cgcctgcgtc gaccccagca ggccagcgcc
     3781 atcgcgatgc cggacgagcc gtgcgcgcag ccgaggtacg cgggatcgga cgccggatcc
     3841 gtcgtccaga tcggaatgcc atcggattcg aaggaacggg cgcggatgcc gtcgaccgcc
     3901 ccgcccgcga cctccagcgg ccagcgctcg tcgagtacgt cgctcagcag gcacgccgtc
     3961 cacgccacgc ccgcggcacc ggagaacagg tcgagttccc gccggtctgc gcaggcgacg
     4021 gtgaatcgct cccgcgcggc ggcgagcaac gactcgtcgc cgaaacggcg cccggtcacc
     4081 gcaagcgcga ttgcgactcc cgcgttgccg gtgaacaggc ctgccgcgtt tccttcggcc
     4141 ggacgcgcgg ccagccagcg cgcaccgccc atgacggcgt cggcgaatcc cgcccggtgc
     4201 gacgcagtgt cgacggtgag caggccgagc acgatccctg cggccccgag gttgatcgcc
     4261 tcgcaatcga aggcacgcat gaaatgcgtg ttgcgccagt gcgtgccttc gcgggtcgtc
     4321 gtggcgtacg tgaccgcgag gcgcgccgag tcgttgcacg catggcgata ccagccgcgc
     4381 tcgggctccg tgcccgggtg accggtcgat gggacgaatt cggcaagact gtccacgcag
     4441 gaagacaacg cggccgcggc ggccgatgcg gtgggccgga tatccggatc gcgagcggtc
     4501 aatgtctcca ccagtcgcgc cgcactgtgc gcgccttcgt tccgcagcag cgcaccgagt
     4561 ccgtgcgacc cggtgccgag cagcgccggc ggggtgtcga gcacggcgtg cgcgacgcag
     4621 gcgccgagcg catagacatc gcgggccggc gtcatcggga cgcggtccac ggtctccggg
     4681 gcgaggtggc cgcgcgtgcc gccgctgcgc gcgatcgtcc ccacgggctc ggccagctcg
     4741 aagtcgatca gtcccacgcc gtcgccgcgg cacacggcat tcgcgagctt gacgtcgccg
     4801 tggacgaatc ccgccgcgtg cacccgcgcc acggcatccg ccagttgcgg gaggcgctcg
     4861 atgcgctggc gccgcggcag ctcggcgagc gactcgccgc gaaagtcctc catcacgagc
     4921 agcggccact cgccgtcctg ccacgccagg gcgcacggcg cgacgccggc ccgcgcggcg
     4981 agcgcggtaa gcacgtcgaa ctcgcgggcc agccgatcgc gcacgtcgag tcccgtcgcg
     5041 tcgccggcaa cgccggggcg cgcggtcttg agaaccacgg tctcgaggct gtcgagatcg
     5101 gcgccgagaa aggtgcgtgc ccgcggactg tcgctcaact gcgcgagcac agtgatgcgt
     5161 cgcgtcgcga cctgcagcgt gtcgccgacg gcgatcggac aggggcgggg aggacagccc
     5221 gcgattggtg gcggcggcgc gcccggcagc tgcgcgccgt cgtcgcgtcg cgtgtcggcg
     5281 gtggtcgagc catccggcag gacgatcgca aaggcgtgca ggccggcact gctcgcgacc
     5341 gtgccgccgc cgccatatgc gccgtaccgg aacgacaccg cggatcccgg gcgcacgtgc
     5401 aggtcggtct gcacctccgg tccgttgctc gcgggccagg cggcgtcgag tcgatcgagc
     5461 agttctcgcg cgtgggcgtc atcgcgcgga tagacacagg cgatcttgcc gagctgctgc
     5521 acgccggcat ccccggaatt caggaacacg acgtcatcga tgctgcgcgg gaccttgaac
     5581 gccgccctct gcgcgcgcgc gacggcgccc acgaccggca gcagcgacgc gctctcggcg
     5641 gcgctggccg acacgtggat cttccagccc tgcgccggcg tcggctcctc gcgtggcgcg
     5701 aagaaccgcc acgacaacgg gccgtcggac cagtcggtcg cgacccagtc gcgcgcgagg
     5761 cgctcgctca gccgtcggag cgcggcggag taggactccg ccgcagcgag cggatcaatg
     5821 ccgtccagca accgcgtcag gggtgatgcg cgtcgcctgg cacgtatgcc atgggcacat
     5881 gtgcagcgtg ccgccatgtc cgtcggcctc ggtgaccggc ggtgcagccg catccttcag
     5941 ctcgacgatg gcatccaccg ggctgaccgc cccggcgtgg accgcgacac gcagatgatc
     6001 gactttcgcc cgcagagcgt cggcagaaac attcatacct gttctccccg ctgttcgatc
     6061 gtattgcagc gcaccaagtt cttacgggtg cggccggaca gcgcacgcgc ctccagcccg
     6121 ggtacggata ccaccgccgc aaatgaaaat caatcagatc tggcgagcgg agccgccaac
     6181 ccccgatgcc gcgtggtcag gtggctggca acgcgttcgg cggcgcctgc agcaccgcga
     6241 aatccgggaa ccgacggcac gcccaggcca ggctcgcgtc gatcgcgtcg cggtgctcct
     6301 cggcagcgga cttcaatcgg gccgcacggt ccgcagtcga aaacgccgcg cgcggcgcct
     6361 tcgagtggac ggccagcgtt gtggcgatcg cggcctcgac gtcgcgggac gagcagccga
     6421 gtcccagaaa gcgtgccagg gccgtcagcg actcctgcgg atcgcgcagg aatcgggcga
     6481 agtcgagcga gcggcagcga ccgccgtgag tcgaccccgc gaatcgagcg tagagccgca
     6541 ggtgcagcac ccagagacag gcagcctggg atgcgtcggg cagcgattgc cacgcctcgc
     6601 gccggaacgc gaactcgtcg ggaaacagca cggcgaggtc gttgagtcgc tcgcgcacga
     6661 accccatgcg cacgcgatcg ccgagcatcg cgagcaggaa ttcctcgaca ccgacgtact
     6721 ggaacagcgc cttcgatccc gggttgagcg cgaggacgtc gcccagcagc tgcgtggacg
     6781 catccgtcgc cttgacgatg gccacctggt cgctgcgata cgtgcgggcc agcagcgcat
     6841 cgagcagtcg cagcgaatcg tgccagccgc cgggcccggc cggatcggca ggtccgtgcc
     6901 ggcgacggtg cgcgaggtcg tggaccgcga acggctcctt gagcacgaac gccgtcgcca
     6961 gttgctcgag gcatctcgac accagcgtcg aaccgcagaa ggcgacgtgg aatatccagt
     7021 gcagcggtcg cggcggagga tgcagttggt ccaacgcgcg gatcgccgcg tcgagcggga
     7081 cctcgcgcga cccgtccatg ggaatctcgc ggtcgtcgag gaaattcacc tcgcgataca
     7141 gttgctccga catcggcacg agcgtgagtg cgttggcctc gggcgcgagc cggatcggga
     7201 agtactccgg cgacagccag agctgcggcc cgtccttcgg tcgtccggcc agtggaggcg
     7261 gatgaatcat gtggcgaatc acgcgaggtg tcgcgggcaa gtctaaaccg actcatccgc
     7321 tgcacgaggg cgcatcgcac gtggcactcg atcgaattgt cccgcggcgg tatcgtggcc
     7381 ggcctgtatg cgcgccacgg gacccagcac gaccggggac cggatcgacc tgccggtgct
     7441 cgacccggcg ttcaaggccg acccgcactc gcgctatgcg cagtggcggg cccagggccg
     7501 gaccatcgtc ccgatccggt tgccgacggg gctcgaggcg tggctcgtga tcgggtacga
     7561 cgcggcacgc gaactgctga acgacccgcg gctgtccaag agtgacccgg gaggcagcgc
     7621 gtccgccgct catcggctgt tccgtcacat gctgacgctc gacccgccgg aacacacgcg
     7681 cctgcgctcc atcatcgcgc gggagttcac ggtgcggcgc gtggccctcc tgcgcccccg
     7741 catccgcgag attgtcgtcc ggttgatcga cgaggcgtcc cgggccgaga cggcggacct
     7801 gatgcaggcg ctggcattgc cactgccact gcaggtgatc tgcgaactca tcggcatgcc
     7861 cgcgggagac gatgcgctcg tgctgcgctg gtcacggcga ctggccgcgg cggacctcga
     7921 tgagccggcg gacgtgcccg ggatcgccga cgaaatccac gggcatctcg cgcgtctcgc
     7981 cgaaggcaag cgacgcgcag cggacgattc gctgctgagc ggcctcgtcg cggcgcagga
     8041 ccgcggggag ctgtccggaa ccgaactcac gtcgatgacc ttcctgctgc tgtcggccgg
     8101 ccacgagacg acggcgaacc tgatcgggaa cggcgtgctc gccctgctgc gcaaccccgc
     8161 gcagtggtcg gcactttgcg ccgacgggga ccacgcgcgc gccatcgtcg aggaggtgct
     8221 gcgcttcgac agcccgctcg aggtgtcgac gatccgccat gccacgagcg agatcgagct
     8281 cggggacgtc cggatccggc ccggcgacac cgtgttcatc gggctggccg ccgccaaccg
     8341 cgacccgagg cgaatcgagc gtgccgggga attcgatcct ggacggcacc ctgccgccgc
     8401 tcacctggct ttcgggcacg gcgtgcactt ttgcgtcggt gccacgctgg cccgcgccga
     8461 gggcgagacc gcgttcgtgg agctgtcgcg ccgatttccg cgcctcgcac tcgacgtgcc
     8521 actcgacgcg cttgaatgga ccccgggcct gatcatgcgc ggaccgctcc gactgccggt
     8581 gcgattcaat tagccgccgc ggaccgaccg tcgagcaacg agcgcacggc tgcgccgtcg
     8641 agttcctgcc gctcgacgag cccctgcacg atgcggtcga gcgccgcgcg ctggtcgcgc
     8701 agcaggtcga tcgccgcgcg gtagcagacg cgcagcaggc cttcgacctg ctcgagctgc
     8761 gcggcggtcg gcgaggacgt ccacagcagg ctgtgatcgc cactgaatcc ggacgtgcag
     8821 atgacgcgcg tcgcgacacg cgtggcgaca gcgaggtcgc tgccgtcccc gccgcccgca
     8881 ctcaggctga cgtcctcctt gccgaagatg acctcctcgg cggcccgtcc ggcgaggatg
     8941 gtgcgcaggc gttcctgcac ttcggtccgc gtcatgaccg cgccttccgc cggcgcggtc
     9001 gcggtgaacc cgagcgagcc gt
//
LOCUS       DAOMFD010000079         8947 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2374_length_8947_cov_23.781826, whole genome shotgun sequence.
ACCESSION   DAOMFD010000079 DAOMFD010000000
VERSION     DAOMFD010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2374_length_8947_cov_23.781826"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..277)
                     /locus_tag="PL152_06175"
     CDS             complement(<1..277)
                     /locus_tag="PL152_06175"
                     /inference="COORDINATES: protein motif:HMM:NF025433.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HQR48902.1"
                     /translation="MWFGAVTYDERAGLSYTTGEVTHHIGSDVDAERDRVVAELTNAG
                     WVGGVQWIDDFHRLGPDGADPQSLKSGRPPACRPRLRRGRRRPVLPLP"
     gene            343..546
                     /locus_tag="PL152_06180"
     CDS             343..546
                     /locus_tag="PL152_06180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48903.1"
                     /translation="MLGDRVAKLPGNTPIVVYCGCCPWSDCPNIAAAYEALRALGFTK
                     IKAPCIAHNFGTDWTDQGCPATR"
     gene            652..1827
                     /locus_tag="PL152_06185"
     CDS             652..1827
                     /locus_tag="PL152_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48904.1"
                     /translation="MRFVSRVLGALALSGVLTTAHAQPPAEQFTDILSSLMMPAGSEG
                     SYDDWSSLEAVKQVRWQPLPPNMLDNALPDGSYFSRKGLANVGGVPFGVVATGARTMV
                     VNVYFRNVSATPMGADLLLDTLRRGGFSLEMARCPVKGTVGAGDTWWRLEAPGKRPAW
                     LSAQTLCNGRKCEGYALLLGDALPTLPPQQRNLYTDQCSGTAAGAPMAPVAAWDAQLA
                     ALFVQLMPPQGAASVPWTAIDAVSGLTWAPMPPHEVPNPVWSDTENHFYRGGQTDLGG
                     RVMYLTATGTRDSVRTIHLEDQSTLANRGDPLAVLRAQGYKVELLRCGKVYLLSSGKW
                     YRVTGRDKHPAMLKREVRCDTVACPKGQETYALALDGKLPRLQAGEVDAVGGSCPGR"
     gene            1853..3313
                     /locus_tag="PL152_06190"
     CDS             1853..3313
                     /locus_tag="PL152_06190"
                     /inference="COORDINATES: protein motif:HMM:NF017076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE2 domain-containing protein"
                     /protein_id="HQR48905.1"
                     /translation="MTDGSSHITRLTSGALRKIRRYVRVSLTLADGGQQTERIRAVTT
                     VAFVALLFAGFGTVRFAMGIAGHFGDVPFEFDRALRTLSVGWFQSERTIPVTLVDIDE
                     ATNRAWGSPAITPRGDLARLLEVVSAAGPAAIVVDIDLSWGDRRTRDAGGAEDAGQRT
                     LQDFLDQYAGRAPIVFPKRIEPAADGLPHAAASPFDDTFQRNSRLAWAHASFETDANG
                     KVREWREWLPYCTGSGTKWLPAVPVSLAANLDPLPDGLQRPTPPTAAGGACGSADARD
                     GVQQRRLLVGPRLSGEGRRRSARAEVVSATVLLDRDVARDDRRLFADRVVLIGATHAS
                     AGDLWLTHAGVIPGVELLANLVRFAPQVFQVPGAMARAVHRVAAIALFLAFAAIQWRY
                     RALVALGVSTILVLAVLAVSIDVFDDFGIFDVIQASILMFVVYKALELVLKLVADVKE
                     RGAVHPPGRRRRTEMLRALYRRKRPLPPGEHDEYVH"
     gene            3297..4181
                     /locus_tag="PL152_06195"
     CDS             3297..4181
                     /locus_tag="PL152_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48906.1"
                     /translation="MSTCTDWGRILAAVVACCACIAPPARAASDDGVGVGVVAEYRPA
                     ASRFKFARPPRGEAVLVQIGTVVVAGDRVTLPANGEVTVQLASGETAKFQGPGTFSVP
                     ASRPLGALATVFQSLGALFDDEYRLSGTAAARGGESCGQDGKVDPIAVPILVPGARIK
                     AGTRDLPMGWRGGCPPFVVTVLSGQDKPVHRESIEGWQVRLDQVPLPVGRYSVVITDS
                     TGKRFDTGLEAADDAPAMPANLAGDTSRLGVTAQAIWLADQDGGRWRLDAFDRLRPLI
                     RAGDPLASSIGDVLLWGH"
     gene            complement(4266..7115)
                     /locus_tag="PL152_06200"
     CDS             complement(4266..7115)
                     /locus_tag="PL152_06200"
                     /inference="COORDINATES: protein motif:HMM:NF024180.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HQR48907.1"
                     /translation="MTRHRRSQSRTADRIAALAVVLVAVLVIVLVAWPSPARATTWIE
                     QAQADLTCTGLADAAERATLAAPWLAAPVTAQSVTRYFRGPPQDSRQAQFRYLTVLAA
                     IGWWAAMVNDQALRSDVYVDIGVLADRYAAGGDALFRQVARCARTQIIVSQVELGEPA
                     LADQVAASLARLYPDTPSAQSAEDWPLLLGLRAIRLEPRASSGIAQLATRSARYAANG
                     VVTTSPERTSRLLAATSQALLALGDAGKARQVALDSLVVTGKPPEVTAAWRAMPVIYD
                     SSVTLSGAKDAANVQVMLRPDAPPPSLNDNEAAFESLLRLSLAADTQERYDDMSRLQQ
                     AAVQRLTNRRGLQTYTMPFQRHALDELAATRDETLGAVARRDPAFAGRTLATYLGLYD
                     TLVRQAQSQFIGDAQEQLFFQYKVDNVLDALAQLSPALPRSKADIDDTTFRLAQLRSY
                     GRLTLATLEGELARSRIDPESRFSVERFFSLSTQTAVWLRALLESVRIAPGMPLPNGE
                     ALWKVFATLDIFYEETAKQYDRYVAFVRQKAPAVAELATPRPLPATEFMRRLRTGEAL
                     VATLVTPRDLYVWAVTTSGVTFVRQSVTARDIEARVQRLRASLKPGGDGTLPPFDAAD
                     AYELYKLVFVPIAPALKGITDIVWYGHGPLGALPPTVLVTSPPAKSQLSSATELAKTP
                     FLVDRYAVAVLADLSLFPWHRDRSVARTQDARFLGVGAPLLTAGEIAGGPRSRSYELA
                     GGLDGRELAQLPKLPESVDELKGLASAAGESNSTLWLGPEASEKQFTGDSLRGYRTIA
                     LATHGFLPGEVRGVPEPALMLALNPQSKDRYDGILTSQEIAALQLDADLVILSACNTA
                     SADGRPRGEAFTGLSQAFFTAGARSLMVSHWPVMSGAAVQLSVSTLERAEKQDLTLSR
                     SLQQAIEEVRKAGASSAIEAHPSYWGAFVIVGDGR"
     gene            7252..7884
                     /locus_tag="PL152_06205"
     CDS             7252..7884
                     /locus_tag="PL152_06205"
                     /inference="COORDINATES: protein motif:HMM:NF018446.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022841 - potassium ion leak channel
                     activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HQR48908.1"
                     /translation="MTTSVDGHHRELRRIEAFSDGVFAIACTLLVLEITVPYVDEATR
                     PGGLWPALQKLWPSFLAYLISFESIFVAWAAHHRFLAVLERSSKPFLYANGLLLVTIT
                     FVPFPTAVLAEYIATPQANVAVMLYSATQLALNLGFVVWMATMLRPVRLLPAAWGPAA
                     VMKIVSQTLSGTAVYVATTIIAYWFPVTGLAVIVGVQVLWIVMSVAESED"
     gene            7974..>8947
                     /locus_tag="PL152_06210"
     CDS             7974..>8947
                     /locus_tag="PL152_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48909.1"
                     /translation="MERTLGPDSAWHVCDDGAVAELHDALCAFAPVGFAHARIVSARC
                     RDLSFYHDHRLVDASLEGVPGIERALLLQGRNDTAWLDGRSLPIHGVNSLESLELDES
                     TVLDYVRFFVFTIRGEHGAFTLIEAADEITLAEVEPAAGGGSAKTPPGEEQLASVRAE
                     WFPLRMLEGSGDGKFRVQGTVAYLSGLFAATFMVEPDGTIEMVEDRPLTSLEGFAVPA
                     AIPLKPHPTDLVPSTTKSALLEALRAHAQAAAAPPATEAERVDDRGATETAVSVLLSE
                     AVRASLGHRLLQRFNSHSQSTGPIGQLGRFLREFAPIIIIESEIPFVE"
BASE COUNT         1350 a         3087 c         3106 g         1404 t
ORIGIN      
        1 tcggcagagg aaggacgggc cgccgccgcc cacggcgtag cctgggtcgg cacgccggag
       61 gccgacccga tttcaggctc tgcggatcgg ccccatccgg ccccaaccgg tggaagtcgt
      121 cgatccactg gacaccgccg acccagccgg cgttcgtcaa ttcggcaacg acccggtccc
      181 gctcggcgtc cacgtccgag ccgatgtgat gcgtgacctc gccggtcgtg tagctcaggc
      241 cggcgcgctc gtcgtacgtc accgcgccga accacaccgg cgagccatcg cgctccggga
      301 tccgaatacg tcggcccggc cagcgagcag gccggtctcc agttgctggg agaccgggtt
      361 gccaagctac cagggaacac gccgatcgtg gtctattgcg gctgctgccc ctggagcgac
      421 tgcccgaaca tcgccgccgc gtacgaggcg ctgcgcgcgc tcggattcac gaagatcaag
      481 gcgccctgca tcgcgcacaa cttcggtacg gactggaccg accagggctg ccccgccacc
      541 cggtagcgca ccggctccgg ccctgcgccc agcggttgca gcggccgtgc attaggcgga
      601 gagttagcgc ccaaagacaa tcaataaccg acgcgccgca ggggggacac gatgcgcttc
      661 gtatctagag tgctgggtgc tctggccctg tccggcgtgc tgacgaccgc ccacgcacag
      721 ccgcccgccg agcagttcac cgacatcctg tcgtcgctga tgatgcccgc cggttcggag
      781 ggatcgtacg acgactggtc gagcctcgag gcggtcaagc aggtccgctg gcaaccgctg
      841 ccgccgaaca tgctcgacaa cgccctgcct gacggctcgt acttctcgcg caagggcctc
      901 gcgaacgtgg gcggcgtgcc cttcggtgtc gtggccacgg gtgcgcgcac gatggtcgtc
      961 aatgtctact tccgcaacgt gagcgcgacc ccgatgggcg cggacctcct gctcgatacg
     1021 ttgcggcgtg gcggattctc gctcgagatg gcgcgttgcc cggtgaaagg caccgtcggc
     1081 gccggcgata cgtggtggcg tctcgaggcg ccgggcaagc ggccggcctg gctgagcgcc
     1141 cagaccctgt gcaatggccg caagtgcgag ggatacgcgc tgctgctcgg tgacgcgctg
     1201 ccgacgctgc cgccgcagca acgcaacctg tacacggacc agtgctcggg cactgcggcc
     1261 ggcgcgccga tggcgccggt cgcggcgtgg gacgcgcaac tcgcggcgct gttcgtgcaa
     1321 ctcatgccgc cgcagggagc cgcatcggtg ccctggaccg ccatcgatgc cgtaagcggc
     1381 ttgacctggg cgccaatgcc gccccatgaa gtgcccaatc ccgtgtggtc ggacaccgag
     1441 aaccacttct accggggtgg acagacggac ctgggtggcc gcgtgatgta cctcaccgcg
     1501 accggcacgc gcgacagcgt gcgcaccatc cacctcgagg accagtcgac actcgccaat
     1561 cgcggtgatc cgctggccgt gctgcgggcg cagggctaca aggtcgagct gttgcgctgc
     1621 ggcaaggtct acctgctgtc gagcgggaag tggtatcgcg tgacggggcg cgacaagcac
     1681 ccggcgatgc tgaagcgcga ggtccggtgc gacaccgtgg cgtgtcccaa gggccaggag
     1741 acctacgcgc tcgcgctcga cggcaagctg ccgcgcctgc aggccggcga ggtcgacgcg
     1801 gtcggcggat cctgccccgg gcgttagcac ggcgagtacg cgcaaccgag ccatgaccga
     1861 cggcagctca cacatcaccc gattgacatc cggcgccctg cggaagattc gtcgctacgt
     1921 gcgcgtctcg ctcaccttgg ccgacggcgg ccagcagacc gagcgcattc gcgcggtgac
     1981 cacggtcgcg ttcgtagcac tgctgttcgc aggattcggc accgtgcggt tcgccatggg
     2041 catcgccggt cacttcggcg acgtgccctt cgaattcgat cgcgcgctgc gcacgctgtc
     2101 ggtcggctgg ttccagtcgg agcgcaccat ccccgtcacc ctcgtcgaca tcgacgaggc
     2161 gaccaaccgc gcatggggat ccccggcgat cacgccgcgc ggcgacctcg cgcgactgct
     2221 cgaagtcgtg agcgccgcgg ggcccgcggc gatcgtggtc gacatcgacc tgtcgtgggg
     2281 tgaccgccgc acgcgcgatg cgggcggagc cgaagacgca gggcagcgca cgctgcagga
     2341 tttcctcgat cagtacgcgg gtcgcgcacc gatcgtgttc ccgaagcgca tcgaaccggc
     2401 ggcggatggc ttgccacacg cggccgcgag cccgttcgac gacacgttcc agcgcaactc
     2461 gcggctggcc tgggcccacg caagcttcga gaccgatgcg aacggcaagg tgcgcgagtg
     2521 gcgcgagtgg ctgccgtact gcaccggctc cggcacgaag tggctgccgg cggtgcccgt
     2581 gagcctggcg gcgaatctcg atccgttgcc cgacgggctg cagcgaccga caccgccgac
     2641 ggcggccggc ggggcgtgcg gatcggccga cgcgcgggac ggcgtgcagc agcggcggct
     2701 cctggtcgga ccgcgcctgt ccggcgaagg gcggcgccgc agcgcccgcg ccgaggtcgt
     2761 ctccgcgacg gtgctgctcg atcgcgacgt ggcgcgcgac gatcggcggc tgttcgcgga
     2821 ccgcgtggtg ctgatcggcg cgacgcacgc gagcgcggga gatctctggt tgacgcacgc
     2881 gggcgtgatt cccggcgtcg agctgctggc gaatttggtg cgcttcgcac cacaggtgtt
     2941 ccaggtgccg ggtgcgatgg cccgcgccgt gcaccgcgtg gcggcgatcg cgctgttcct
     3001 ggcgttcgcc gcgatccagt ggcgatatcg ggcgctcgtt gcgctcggcg tgtccacgat
     3061 cctcgtgctg gcggtgctcg ccgtgtccat cgacgtgttc gacgacttcg ggatcttcga
     3121 cgtaatccag gcgtcgatcc tgatgttcgt cgtgtacaag gccctggagc tcgtgctgaa
     3181 gctggtcgcg gacgtgaagg agcgcggggc ggtacacccg cccgggcgcc gacgacggac
     3241 tgagatgctg cgcgcgctgt acaggcgcaa gcgcccactc ccaccgggag aacacgatga
     3301 gtacgtgcac tgactggggc cggatcctgg ccgcggtcgt ggcctgctgc gcttgcatcg
     3361 cgccgccggc ccgcgcggcg agcgacgacg gcgtcggtgt cggtgtcgtc gccgaatatc
     3421 gcccggcggc gagccggttc aagttcgcac gcccaccgcg cggcgaagcg gttcttgtgc
     3481 agatcggcac cgtggtggtc gcaggcgacc gggtcacgct gcccgcgaat ggcgaggtga
     3541 ctgtgcaact cgcgagcggc gagacggcga agttccaggg accgggcacg ttctcggtgc
     3601 cggcgtcgcg tccactcggc gcattggcga cggtgttcca gtccctcggc gccctgttcg
     3661 acgacgagta ccggctgtcg ggcaccgcgg cggctcgcgg cggggagtcc tgcggacagg
     3721 acgggaaggt ggatccgatc gcggtgccga tcctggtgcc gggcgcccgg atcaaggcag
     3781 gcacgcgcga cctgccgatg ggctggcgcg gcggctgtcc accgttcgtg gtcacggtgc
     3841 tgtccggtca agacaagccg gtgcatcgcg aatcgatcga aggctggcag gtgcggctcg
     3901 accaggtgcc gctgccggtc ggccgttact cggtcgtgat cacggactcc acgggtaagc
     3961 gcttcgacac cggtctcgag gctgcggacg atgcgccggc gatgccggcc aatcttgcgg
     4021 gcgacacgag ccggctcgga gtcacggcac aggcgatctg gctcgccgac caggacggcg
     4081 gccgctggcg cctcgatgcc ttcgatcggc tgcgaccgct gatccgcgcc ggcgatccgc
     4141 tcgcaagcag catcggcgac gtcctgctct ggggccactg atccggggac atggaaattg
     4201 ggaaattggg gacattctcc tatttcgcct cgaaatagga gaatgtcccc aatttccatg
     4261 tccctctagc ggccgtcgcc gacgatcacg aacgcccccc agtaggaagg gtgcgcctcg
     4321 atcgcgctcg aggcgccggc cttgcgcacc tcctcgatcg cctgctgcag gctccgcgac
     4381 agcgtgaggt cctgcttctc cgcgcgctcg agcgtactca ccgagagctg cacggcggcg
     4441 ccggacatca ccggccagtg actcaccatg agcgaacggg cgcccgccgt gaagaacgcc
     4501 tggctcaagc cggtgaacgc ctcgcctcgc ggtcgtccgt ccgcgctcgc ggtgttgcac
     4561 gcggacaaga tcaccaagtc cgcgtcgagc tgcagcgcgg cgatctcctg actggtcagg
     4621 attccgtcgt agcgatcctt gctctgcgga ttgagcgcca gcatcagcgc cggttccggc
     4681 acgccgcgca cctcgccggg caggaacccg tgcgtcgcaa gcgcgatcgt ccggtagccg
     4741 cgcagtgaat caccggtgaa ctgcttttcg ctcgcctcgg gaccgagcca gagcgtcgag
     4801 ttggactcgc ccgcagcgga ggccaggccc ttcaactcgt ccaccgattc cgggagcttc
     4861 ggcagttgcg cgagctcgcg gccatccagg ccgccggcta attcatacga gcgcgagcgg
     4921 ggtccgcccg caatttcgcc agccgtgagc agcggtgcgc ccacgcccag gaatcgagcg
     4981 tcctgcgtcc gcgcgacgct gcgatcgcga tgccacggga acagcgacag gtctgcgagc
     5041 acggcgactg cgtagcggtc gacgaggaac ggtgtcttcg cgagctcggt cgcgctcgac
     5101 agctgcgact tcgccggggg tgaagtcacc agcacggtcg gtggtagcgc gccaagcggc
     5161 ccgtgaccat accagacgat gtccgtgatg cccttgagcg ccggggcgat cggcacgaac
     5221 acgagcttgt acagttcgta cgcgtcggcg gcgtcgaagg gcggcagcgt gccgtcgccg
     5281 cccggcttca ggctcgcgcg caggcgctgg accctggcct cgatgtcgcg tgcggtcacg
     5341 gactggcgca cgaacgtgac gccgctcgtc gtcacggccc agacgtacag gtcgcgcggg
     5401 gtcacgagcg tggcgacgag ggcctcgccg gtcctgagcc gtcgcatgaa ttcggtcgcc
     5461 ggcaatggtc gtggcgtggc gagttccgcc accgccggcg ccttctgccg cacgaatgcg
     5521 acgtagcggt cgtactgctt cgcggtctcc tcgtagaaga tgtcgagcgt ggcgaacacc
     5581 ttccacagcg cctccccgtt cggcagcggc atccccggcg cgatgcgcac cgactcgagc
     5641 agcgcccgca accacaccgc cgtctgcgtg gacagcgaga agaatcgctc gacgctgaac
     5701 cgagactctg gatcaatcct gcttcgtgcc agctctccct cgagcgtcgc gagcgtgagc
     5761 cggccgtagg accgcagctg cgcgaggcgg aatgtcgtgt cgtcgatgtc cgccttcgaa
     5821 cgcggcagtg ccggggacag ctgcgcgagt gcgtcgagga cgttgtcgac cttgtactgg
     5881 aagaacagct gctcctgcgc gtcaccgatg aactgcgact gcgcctggcg gaccagcgtg
     5941 tcgtagagac ccaggtaggt cgcgagcgtc ctgcccgcga acgccgggtc acggcgtgca
     6001 acggcgccga gcgtctcgtc gcgcgtggcc gcgagctcgt cgagtgcgtg gcgctggaac
     6061 ggcatcgtgt aggtctgcag cccgcgccgg ttcgtcaggc gctgaaccgc ggcctgctgc
     6121 aatcggctca tgtcgtcgta ccgctcctgc gtgtccgccg ccagcgaaag gcgcaacaac
     6181 gactcgaacg ccgcctcgtt gtcgttcagc gacggtggtg gtgcatctgg ccgcagcatg
     6241 acctgcacgt tggcggcatc cttcgcacca ctcagcgtca ccgaagagtc gtagatcacc
     6301 ggcatcgcgc gccatgcggc cgtgacctcg ggcggcttgc ccgtgacgac cagcgaatcg
     6361 agcgcgacct ggcgcgcctt gcccgcgtcg cccagcgcca gcaatgcctg gctcgtcgcc
     6421 gcgagcagtc ggctcgtgcg ctccggcgag gtcgtgacga ccccgttggc cgcgtacctt
     6481 gccgagcggg tcgcgagctg cgcgatcccg ctcgaggcgc gcggctcgag gcggatcgcc
     6541 cgcaagccca gcagcaacgg ccaatcctcc gccgactgcg ccgacggcgt gtccggatag
     6601 aggcgtgcaa ggctcgcggc gacctggtcg gccagcgcgg gctcgccgag ctcgacctgc
     6661 gagacgatga tctgcgttcg tgcgcaacga gccacctggc ggaacagcgc gtcgccaccc
     6721 gcggcgtagc gatcggcgag tacgccgatg tcgacgtaca cgtcgctgcg caacgcctgg
     6781 tcgttgacca tggccgccca ccagccgatg gcggccagca ccgtcaggta gcggaactga
     6841 gcctggcgcg agtcctgcgg cggtccacgg aaatagcggg tgacggattg cgccgtgacc
     6901 ggcgccgcga gccacggcgc agcgagcgtc gcgcgctcgg cagcatcggc aagcccggtg
     6961 cacgtcaggt cggcctgggc ctgctcgatc catgtcgtcg cccgggctgg actcggccat
     7021 gcgacgagca caatgacgag cactgcgacg agcacgacgg caagcgcggc aatccggtcc
     7081 gcagtccggg actgacttcg acggtgacgg gtcatctccc cctcccccac cgctctcgtt
     7141 gcgcgcagca tcgcagactc cgtcgcacaa agcgacaggc tccggtacat actggtccac
     7201 gttggccagg ccgtcagctc cgactgccac ctgccgcaag cgagccgcgc catgacgaca
     7261 tccgttgatg gacatcacag ggaactgcga cggatcgagg ccttcagcga cggggtgttc
     7321 gcgatcgcct gcacgctgct ggtactggag atcaccgtgc cgtacgtgga cgaggcgacg
     7381 cgtcccggcg ggctctggcc ggcactgcag aaactgtggc cttcgttcct ggcgtacctg
     7441 atcagcttcg agtcgatctt cgtcgcctgg gcggcacacc accgattcct ggccgtgctc
     7501 gaacgatcgt cgaagccgtt cctctacgcc aacgggctcc tgctggtgac catcacgttc
     7561 gtcccgttcc cgacggccgt cctggccgag tacatcgcca cgccgcaggc caacgtggca
     7621 gtgatgctct acagcgcgac acagctggcc ctgaatctcg gcttcgtcgt gtggatggcc
     7681 accatgctcc ggccggtgcg cctgctgccg gcggcctggg gtcccgccgc cgtgatgaag
     7741 atcgtgagcc agacactcag cggcacggcg gtctacgtgg ctacgacgat cattgcgtac
     7801 tggtttcccg tcaccgggct cgccgtcatc gtcggggtcc aggttctatg gatcgtcatg
     7861 tcggtcgccg agtccgagga ctgatgctgc ggtcgcagtc ggcaagacgg ccatggacgt
     7921 tgtagagtcg cagtcgttca ttgcgcgttg ggtgcccggg gggagtacac gacatggaac
     7981 gaacactcgg gccggacagc gcgtggcacg tgtgcgacga cggcgcggtc gcggagttgc
     8041 acgacgcgtt gtgcgccttt gcgccagtcg ggttcgcgca cgcgcgcatc gtgagtgcca
     8101 ggtgtcgcga cctgtccttc taccatgatc accgcctggt cgacgcgtcg ctcgagggcg
     8161 tcccggggat cgagcgcgcc ctgttgctgc agggccggaa cgacacagcg tggctcgacg
     8221 gccgctcgct gccgatccac ggcgtcaact ccctcgaatc gctcgaactc gacgagtcca
     8281 cggtgctcga ctacgtgcgg ttcttcgtct tcaccatccg gggcgagcac ggggccttca
     8341 cgctgatcga agccgcggac gagatcacgc tggccgaggt cgagcccgcc gccggcggcg
     8401 gctccgccaa gacgccaccg ggcgaggaac agctcgcgtc cgtgcgcgcc gaatggttcc
     8461 cgttgcggat gctcgaaggg tccggggacg gcaagttccg cgtgcagggt accgtcgcgt
     8521 acctgagcgg tttgttcgcg gcgacgttca tggtcgagcc cgacggcacg atcgagatgg
     8581 tggaggacag gccactcacc agcctcgagg gattcgccgt tcccgcggcc atccccctca
     8641 agccgcatcc caccgacctg gtgccgtcga cgaccaagtc ggccctgctc gaggcgttgc
     8701 gggcccatgc tcaggccgcc gcggccccgc ccgcgacgga agccgagcgc gtcgacgacc
     8761 ggggcgcgac cgagacggcg gtctccgtcc tgctgtcgga ggcggtgcgc gcgagcctcg
     8821 gccatcgcct gctgcagcgc ttcaacagcc actcgcagtc gaccgggccg atcgggcagc
     8881 tgggccggtt cctgcgcgag ttcgcgccga tcatcatcat cgagtcggag atccccttcg
     8941 tcgagga
//
LOCUS       DAOMFD010000080         8886 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2412_length_8886_cov_33.500623, whole genome shotgun sequence.
ACCESSION   DAOMFD010000080 DAOMFD010000000
VERSION     DAOMFD010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8886)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8886)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8886
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2412_length_8886_cov_33.500623"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="PL152_06215"
     CDS             <1..248
                     /locus_tag="PL152_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48910.1"
                     /translation="GKVPKDRDPKSLTLEECTAILAAAPERKGRWGKKTVAKAPSADA
                     AQGAATDVAARPARKTAARKRASKSKATAAKTPKRKS"
     gene            301..1230
                     /gene="hemF"
                     /locus_tag="PL152_06220"
     CDS             301..1230
                     /gene="hemF"
                     /locus_tag="PL152_06220"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004109 - coproporphyrinogen oxidase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HQR48911.1"
                     /translation="MTAATQSTATVIAYLRSLHDRVTAVLEAADGDATFRRDSWQRPE
                     GGGGESRVLRDGAVFEQAGINFSHVMGATLPASATAARPELAGASFEATGVSLVIHPR
                     NPYVPTSHANVRHFVAMRDGEPAAWWFGGGFDLTPYYPFEDDVLAWHRAAKAACDPFG
                     PDVYAKYKDWCDRYFYLKHRGETRGVGGLFFDDLNEWGFEKSFAFIRSVGDAYAAAYL
                     PIVDKRKDTPYTERERNFQLYRRGRYVEFNLLYDRGTVFGLQTGGRTESILMSLPPLV
                     RWEYDYRPEPGTPEARLGRDFLRPRDWLQDLGA"
     regulatory      1425..1510
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1668..4715
                     /locus_tag="PL152_06225"
     CDS             1668..4715
                     /locus_tag="PL152_06225"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HQR48912.1"
                     /translation="MHKKQLLTMLATTAFAVTAALSVPTSQLSAATSGKARPASASNK
                     AYASQGREAWTPKANGVFIVRMADAPVAGYDGRIKGYAATRPAAGSKIDLARPETQNY
                     RALLRQKQDAVLAKVGGQKIYSYDLVFNGFAAKLTDAQAEKLATMPGVLAVTRDEVRS
                     ADTSSTPAFLGLTQPGSLWWWAGGPRNAGDGVVVGIIDSGITPENPSFANVDRHSRGH
                     GGRHQHHRGDDDGHGYGHSDPGVHGWTGYCEDGEDFVGEDACNGKIIGARYFNEAWGG
                     DAAIEADLPWEFTSPRDYDGHGTHTSGTAAGNYKTPVTGPAAVYGPVSGMAPGARIAM
                     YKALWSVEDASTANGYTSDLVAAIDQAVADGVDVINYSVSGTTTNFRDPVEISFLFAA
                     DAGVFVAASAGNSGPTTGTVAHPSPWITTVAAGTHNRSAVDTVTLGNGVTYTGVGLAS
                     SPVTAPLIYSSAAAAAGADPTAARLCFSTGTAGGTAQLDPALVAGKIVVCDRGTNARV
                     DKSLAVQEAGGVGMILVNTSPNSLNADFHSVPTVHLQNTDRAAVLAYAATSGATATIN
                     TVVFDYSTPAPYTASFSSRGPLTAGAGDLLKPDIIAPGQDILAAFSPSVGGLDFNLLS
                     GTSMSSPHIAGLAAVMKDVHPRWSPMMIKSALMTTATDVLDGPPTDPTVIFRQGAGHV
                     APNKAIDPGLVFDSGWNDWLGFLCGTQLPAVNCTSSGIPVLDPSDFNSPSIAIGDLAG
                     SQTVTRTLKNVSWRSTYKVSVTGLEGTTVTVSPSTFTINPNKTQKVSITVTRTTAALN
                     AYVGGYITWSDGWHKVRIPVVVKPVALGAPAQVSGSYTVKFGYDGPFSATARGLVPAM
                     TFPGTVSNGAYAGFVVPVPAGTTYARFSLFDTDVSPASDLDLRVFRINPDNTLTQVGG
                     SGGGTSAEEVNLLNPTAANYYMVVDGYATANPSSFTAYAWALGGADAGNMTVTAPAAA
                     ALGATGNIGLSFTGLTPGVKYLGSVAYGGAASLPNPTIVRVDP"
     gene            complement(4794..5228)
                     /locus_tag="PL152_06230"
     CDS             complement(4794..5228)
                     /locus_tag="PL152_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48913.1"
                     /translation="MRRKRVGPGHLSLGWAAGCACTIGLAALAACAADSSAPPGGAPQ
                     PVAVGVAAVIEKALADAEQRTGLPRNQLKVESAESVTWPDGSLGCPKPGMAYTQALVP
                     GYRVRIRAAGELLDYHAGRGGTPALCPAELARDPLPDGDPTR"
     gene            5250..6101
                     /gene="rlmJ"
                     /locus_tag="PL152_06235"
     CDS             5250..6101
                     /gene="rlmJ"
                     /locus_tag="PL152_06235"
                     /inference="COORDINATES: protein motif:HMM:NF016278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HQR48914.1"
                     /translation="MNYRHAFHAGNFADVLKHVVLMMLVEHLKKKPAPFFFLDTHAGR
                     GQYDLSEAQPQRSGEYQQGIGRLLGIREATLPAEVADYVALVRARAGPARSPITAYPG
                     SPTIVAMLRRPTDRLVLVEKEPREADALRTALGRQRRVSVVEGDGYGALKAHLPPREN
                     RGLVLIDPPYESDAEFDHVLAGLELAHERWPTGTCCVWYPHTDRAGPARFHRELERSG
                     LRKVLDVWLGILPPDATVGMGAAGLVIVNPPWQLDERLKLLLPRLHALLSPDGSGDCG
                     VEWIVPE"
     gene            complement(6156..6764)
                     /locus_tag="PL152_06240"
     CDS             complement(6156..6764)
                     /locus_tag="PL152_06240"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HQR48915.1"
                     /translation="MAEKHPYAPDARPRCQWTYGGMSAEYVRYHDEEWGVPAHDDRAQ
                     FEFLVLEGAQAGLSWSTILRKRAGYREAFADFDVEKVARYDKRKVERLMNFPGIVRNR
                     LKIESAVSNAKAVLKLQDEVGSLAKHLWAFVGGRPIVNRWASMQQVPATSRESDALSK
                     DLKQRGFRFVGSTILYAHMQATGMVNDHTVDCYRHAVCARMK"
     gene            complement(6781..7467)
                     /locus_tag="PL152_06245"
     CDS             complement(6781..7467)
                     /locus_tag="PL152_06245"
                     /inference="COORDINATES: protein motif:HMM:NF023287.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3379 family protein"
                     /protein_id="HQR48916.1"
                     /translation="MDCREFRREAGADPAHLGEEAAAHRDGCPACAEFLRQTLALDDR
                     IRAALRVPVPKRGRAGRALPAVAPRRWLALAASIVSGVAIGSLLWVAGPRGSLAEDLV
                     KHLAHEPGAMVATEVAASPTTVAEVLDRAGVRLRPGVGVVSYANTCPFRGDKVPHLVV
                     QTATGPVTVMVLRHERVAHPVQFDEGGYAGTIVPVGPGSIAVIGHQPAADLDAIARQL
                     SGAVEWIPGG"
     gene            complement(7470..7991)
                     /locus_tag="PL152_06250"
     CDS             complement(7470..7991)
                     /locus_tag="PL152_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR48917.1"
                     /translation="MNAPFAGKRGESAFARLCDELRPDLYRFAYWLARDRDVAEDVVQ
                     EALLRAWRSRQALETEAAAKPWLLTIVRREHARLYERRRFETVPVDDLVGSESPELGV
                     AEDQETLHVRRAILALEDDYREPLVMQVLLGHTTDEIAAQLGLTRGAVLTRLFRARQK
                     LKDRLGLAGGEEE"
     gene            8202..>8886
                     /gene="hemB"
                     /locus_tag="PL152_06255"
     CDS             8202..>8886
                     /gene="hemB"
                     /locus_tag="PL152_06255"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HQR48918.1"
                     /translation="MRETTVTASDFIYPVFVMEGAAQRVPVPSMPGVERMTVDELVRE
                     AEAVAKLGVPAVALFPVTASEAKSLDGREAWNPDGLAQRAVRALKKAVPGLGVITDVA
                     LDPFTTHGQDGIIDESGYVVNDVTVEALVKQALSHAAAGADVVAPSDMMDGRIGAIRD
                     ALEAAGHIHTRILAYSAKYASSFYGPFRDAVGSAGNLGKGNKYTYQMDPGNSDEALRE
                     VALDLEEGAD"
BASE COUNT         1418 a         3132 c         3002 g         1334 t
ORIGIN      
        1 acggcaaggt cccgaaggat cgcgacccga agtcgctcac actcgaggag tgcactgcga
       61 ttctcgccgc cgcgccggag cgcaagggtc gctgggggaa gaagaccgtg gccaaggcgc
      121 ccagcgcgga tgccgctcag ggagcagcga cggacgtcgc agccaggccc gcacggaaaa
      181 ccgcggccag gaagagggca agcaagagca aggcaactgc tgcgaagact ccgaaaagga
      241 agtcgtgacg ttttcctttc gcgttcccgt tgaccgggcc gctccgcacc tcgtgagccc
      301 atgacagccg ccacgcaatc gaccgccact gtcatcgcct acctgcgctc gctgcacgat
      361 cgcgtcaccg ccgtgctgga agcggcggac ggcgacgcga cattccgccg cgattcgtgg
      421 caacgccccg agggcggcgg cggcgagagc cgcgtgctgc gcgacggcgc cgtgttcgag
      481 caggctggca tcaatttctc gcacgtcatg ggcgcgacgc tgcctgcgtc cgccaccgca
      541 gcgcgacccg aactcgcggg tgcgagcttc gaggccactg gcgtgtcgct cgtgatccac
      601 ccgcgcaatc cctacgtgcc gacgtcgcat gccaacgtgc gccatttcgt cgccatgcgc
      661 gacggagaac ccgccgcgtg gtggttcggc gggggattcg acctgacgcc ctactacccg
      721 ttcgaggacg acgtgctcgc gtggcaccgc gcggcgaagg cggcgtgcga ccccttcggg
      781 cccgacgtgt acgcgaagta caaggactgg tgcgatcgct acttctacct gaagcaccgc
      841 ggcgagactc gcggggtcgg cgggcttttc ttcgacgacc tgaacgagtg gggcttcgag
      901 aagtcgttcg ccttcattcg cagcgtcggt gacgcgtatg cggctgcata cctgccgatc
      961 gtcgacaagc ggaaggacac cccgtacacc gagcgagagc gtaatttcca gctttaccgg
     1021 cgcggacgtt atgttgaatt caacctgctg tacgaccgtg gcacggtctt cggactgcag
     1081 accggagggc ggaccgagtc gatcctcatg tcgctcccgc cgctggtccg ctgggaatac
     1141 gactaccggc ccgaaccagg cacgccggag gcgcggctcg gcagggattt cctgcgaccg
     1201 cgggactggc tgcaggatct cggtgcctga cggcggaccg cgcccgcggt gacatggacc
     1261 ggcccggttg cgactggcgc cgccggccca cgttccaggg cgcctgtcca tcacgacgac
     1321 gagggggaga cgaccccact ctttttatgt cgcgccgcag cgcaacaagg cccggggacg
     1381 gcttgttttc cccaatggac ctgcgtactt tccgctccac ggcaagcgtc gaagggcaca
     1441 cccgctgcga agcggggtcg caaagccaca gctcttgatc aagaaagaac aagagggctg
     1501 ggccgcctga gggagggact gcctggcacc gcatcgcggg cctggcagcc atcggtactt
     1561 cagcacgacg gtggttcgct gtgcggcgca cgcgtgcgcg cgcattggca ttcaccgcgc
     1621 gattgccaag gggtccaatc gttgaagtgc actgccggag ggctcacatg cacaagaagc
     1681 aactgctgac gatgctggcg acgaccgcgt tcgcggtcac cgccgcattg tccgttccca
     1741 cgtcccagct gagcgccgcg accagcggca aggcgaggcc ggcgagcgcc agcaacaagg
     1801 cgtacgcgtc gcagggccgg gaggcctgga cgccgaaagc caacggcgtg ttcatcgtca
     1861 ggatggccga tgccccagtg gccggttacg acggccgcat caaggggtac gcggcgacgc
     1921 gtccggcggc cggcagcaag atcgaccttg cgcgtccgga gacccagaac taccgcgcgc
     1981 tcctccggca gaagcaggac gcggtgctcg cgaaagtcgg tggccagaaa atctacagct
     2041 acgacctcgt gttcaacgga ttcgcggcca agctgaccga tgcgcaggcg gagaagctcg
     2101 cgacgatgcc gggcgtgctc gcggtcacca gggacgaggt gcgcagcgcc gacacgtcct
     2161 cgactccggc gttcctgggc ctcacgcagc ccggcagcct gtggtggtgg gccggcggcc
     2221 cccgcaacgc gggcgacggg gtcgtggtcg gcatcatcga ttccggcatc acgcccgaga
     2281 acccgagctt cgcgaacgtg gatcgccact cgcgtggaca cggtgggcgc caccagcacc
     2341 atcgcggcga cgatgacggg cacggctacg gccacagcga cccgggcgtc cacggatgga
     2401 ccggttactg cgaggacggc gaggacttcg tcggtgaaga cgcctgcaat ggcaagatca
     2461 tcggcgcgcg ctacttcaac gaagcgtggg gcggtgacgc cgcgatcgag gcggacctcc
     2521 cctgggagtt cacgtccccg cgcgactacg acggccatgg cacccacacg tccggcaccg
     2581 cggccggcaa ttacaagacc ccggtgaccg gcccggcggc ggtttacggc ccggtgagcg
     2641 gcatggcgcc gggcgcccgc attgccatgt acaaggcgct gtggtcggtc gaggatgcct
     2701 cgacggccaa cggctacacg tcggacctcg tagccgccat cgaccaggcg gttgccgacg
     2761 gcgtggacgt catcaattac tccgtgagcg gcacgaccac caatttccgc gacccggtcg
     2821 agatttcgtt cctgttcgcg gccgacgcgg gcgtgttcgt cgcagcctcg gccggcaaca
     2881 gcggcccgac gaccggcacg gtcgcgcacc cgagcccgtg gatcacgacc gtcgcggccg
     2941 gcacgcacaa tcgcagcgcc gtcgacacgg tcaccctggg caacggcgtg acctacacgg
     3001 gcgtgggcct cgcttcctcg ccggtgactg ccccgctgat ctactcgagc gctgcagcgg
     3061 ctgccggtgc ggatccgacc gcggcgaggc tctgcttctc gacgggtacg gctggcggca
     3121 ctgcgcaact cgacccggcg ctcgtggccg gcaagatcgt ggtctgtgac cgcggcacga
     3181 atgcgcgagt cgacaagagc ctcgcggtgc aggaggccgg cggcgtcggc atgatcctgg
     3241 tgaacacgtc gccgaattcg ctgaatgcgg acttccattc ggtgccgacg gtgcacctgc
     3301 agaacaccga ccgcgcagcg gtgctcgcct acgcggcaac gtcgggtgcc acggcaacta
     3361 tcaacaccgt ggtgttcgac tactcgacac cggcgccgta cacggcatcg ttctcgtcgc
     3421 gtggcccgct cacggcgggc gccggcgacc tgctcaagcc cgacatcatc gcgccggggc
     3481 aggacatcct cgcggccttt tcgccgagcg tcggcggcct cgacttcaac ctgctgagcg
     3541 gcacctcgat gtcgtcgccg cacatcgccg gactcgcggc cgtgatgaag gacgtgcatc
     3601 cgcgctggtc gccgatgatg atcaagtcgg ccctcatgac gacggccacc gatgtgctcg
     3661 acggcccgcc gaccgacccg accgtgatct tccggcaggg cgccggtcac gtcgcgccga
     3721 acaaggcgat cgacccgggc ctcgtgttcg actccggctg gaacgactgg ctcggcttcc
     3781 tgtgcggcac gcagctgccg gcggtgaact gcaccagctc cggcatcccg gtgctcgacc
     3841 cgagcgactt caacagcccg tccatcgcga tcggcgacct cgccggctcg cagaccgtga
     3901 cgcgtacgct gaagaacgtc agctggagga gcacctacaa ggtgtcggtg acgggcctcg
     3961 agggcacgac ggtcacggtc tcgccgtcga ccttcacgat caaccccaac aagacgcaga
     4021 aggtgtcgat taccgtcacg cggacgacgg cggcgctgaa cgcgtacgtg ggcggctaca
     4081 tcacctggag cgatggctgg cacaaggtcc gcatcccggt ggtcgtcaag ccggtcgcgc
     4141 tcggcgcccc ggcgcaggtg agcggctcgt acacggtgaa gttcggctac gacggtccgt
     4201 tctcggccac ggcccgcggg ctggtgccgg caatgacctt cccgggcacc gtgagcaacg
     4261 gcgcgtatgc ggggttcgtg gtgccggtcc cggccgggac gacgtatgcc cgcttctcgc
     4321 tgttcgacac cgacgtcagc ccggcgagcg acctcgacct gcgcgtgttc cggatcaacc
     4381 cggacaacac gctcacgcag gttggtggga gcggtggtgg cacgtcggct gaggaggtca
     4441 acctcctcaa cccgaccgcg gccaattact acatggtggt cgacggctat gcgacggcga
     4501 acccgtcgag cttcacggcc tacgcatggg cactgggcgg tgccgacgcc ggcaacatga
     4561 cggtgacggc gccggccgcc gcggctctgg gcgcgaccgg caacatcggc ctgtcgttca
     4621 cgggcctgac gccgggcgtc aagtacctcg gttcagtcgc ctacggcggc gctgcgagcc
     4681 tgccgaaccc gacgatcgtg cgcgtcgacc cgtaacgggc cggccacacg agtcgtgacg
     4741 actcgcgccc cggggagaaa tccccggggc gcttttcttt gggcgagcgc tgttcagcgc
     4801 gtgggatcgc cgtcgggcaa tggatcgcgg gcgagttcgg ccgggcatag cgcgggcgtt
     4861 ccgccacggc ctgcgtggta gtcgagcaac tcccctgcgg cgcgaatcct gacccgatag
     4921 ccgggaacca gtgcctgtgt gtaggccatg ccgggtttcg ggcacccgag cgatccgtcg
     4981 ggccaggtaa ccgattccgc cgattcgacc ttgagctggt tgcgcggcaa gccggtgcgc
     5041 tgctcggcat cggccagcgc cttttcgatc accgcagcga ccccgacggc gaccggctgc
     5101 ggcgcgcctc ccggcggcgc cgaggaatca gcggcgcacg ccgcgagcgc ggccaggccg
     5161 atggtgcatg cgcatccggc agcccagccg aggctaagat gaccgggtcc gactcgtttc
     5221 ctgcgcatcg tacttccctc cgtaccccga tgaactaccg ccacgccttc cacgccggca
     5281 actttgcaga cgtcctgaag cacgtagtgc tcatgatgct cgtcgagcac ctgaagaaga
     5341 agcccgcgcc gttcttcttc ctcgacaccc acgccggccg cggccagtac gacctgagcg
     5401 aggcgcagcc gcagcgcagc ggcgagtacc agcagggaat cggccgcctg ctcgggatcc
     5461 gcgaggctac gctgcccgcc gaggtcgccg actacgtcgc gctggtccgc gctcgcgcgg
     5521 gcccggcgcg cagtccgatc acggcctacc cggggtcgcc gacgatcgtg gcgatgctgc
     5581 gccggccgac cgaccggctc gtgctcgtcg agaaggaacc gcgggaggcg gacgccctgc
     5641 gcacggcgct cggccggcag cggagggtgt ccgtcgtcga gggagacggt tacggggcgc
     5701 tgaaagccca tctgccgcca cgcgagaacc ggggcctggt gctgatcgac ccgccctacg
     5761 agtccgacgc cgagttcgac cacgtgctcg cgggactgga actcgcgcac gaacgctggc
     5821 ccacgggcac atgttgcgtc tggtacccgc acacggatcg cgccgggccc gcgcgctttc
     5881 accgcgaact cgagcgatcc ggcctccgca aggtcctcga cgtgtggctg ggcatcctcc
     5941 ccccggacgc gaccgtcggc atgggcgccg cgggcctcgt gatcgtcaat ccgccgtggc
     6001 aactcgatga acggctgaag ctgctgctgc cccggctgca cgcgctgttg tcgccggacg
     6061 ggtcgggcga ttgcggcgtg gagtggatcg ttcccgaatg acgcgggacg cctgacctcg
     6121 ggccggacgt gccgagatgc ggctcggcgc cgccctcact tcatcctggc gcacacggcg
     6181 tggcgatagc agtccaccgt gtggtcgttg accatgccgg tcgcctgcat gtgtgcgtaa
     6241 agaatcgtcg aaccgacgaa gcggaagcct cgctgcttca ggtccttgct cagcgcatcc
     6301 gactcccggc tcgtggcagg cacctgctgc atcgaagccc agcgattcac gatcggccgg
     6361 ccgccgacga acgcccacag gtgtttcgcc agcgacccga cctcgtcctg cagcttcagc
     6421 acggctttcg cgttggagac ggcggattcg atcttcaggc gattgcgcac gatgcccggg
     6481 aaattcatga ggcgctcgac tttccgcttg tcgtagcgcg cgaccttctc gacgtcgaag
     6541 tccgcgaacg cctcgcgata tcccgcgcgc ttgcgcagga tcgtggacca gctcaaccct
     6601 gcctgcgcgc cttccagcac caggaactcg aactgggcgc gatcgtcgtg ggccgggacg
     6661 ccccattcct cgtcgtggta ccggacgtac tccgcgctca tgccgccgta ggtccactgg
     6721 cagcgcgggc gggcgtcggg ggcgtatggg tgcttctcgg ccaaggtcct gtctcccgtg
     6781 tcagccgccg gggatccact ccacggcacc actcaactgc cgcgcgatcg cgtcgaggtc
     6841 cgcggccggc tggtgaccga tcaccgcgat gctgcccgga cccaccggga cgatcgttcc
     6901 cgcatagccg ccttcgtcga actgcaccgg atgggcgacc cgctcgtgcc gcagcaccat
     6961 gaccgtgacg gggcccgtcg ccgtctgcac gacgaggtgc ggaaccttgt cgccgcggaa
     7021 tgggcaggtg ttcgcatagc tgacgacgcc gaccccgggg cgcagccgga cacccgcgcg
     7081 gtccaggact tcggcgacgg tggtcgggct cgcggcgacc tcagtcgcca ccatggcacc
     7141 cggctcgtgg gccaggtgct tcacgaggtc ttcggccagc gatccgcgag gcccggcaac
     7201 ccacaggagc gacccgatcg cgaccccgct gacgatgctg gccgcgagcg cgagccagcg
     7261 gcgcggtgca accgctggta atgcccggcc tgcccgcccg cgcttcggca ccggcacgcg
     7321 cagcgcggcc cggatcctgt cgtccagcgc gagggtctga cgcaggaatt ccgcgcacgc
     7381 cggacagccg tcacgatgcg cggccgcctc ctcgccgagg tgggccgggt ccgcccctgc
     7441 ttcgcgccgg aactcgcggc aatccatgct cactcctcct cgccaccggc caggccgagc
     7501 cggtccttga gcttctgccg cgcccggaac agccgggtga ggacggcgcc gcgcgtgagc
     7561 ccgagctgcg ccgcgatctc gtccgtcgta tgcccgagca gcacctgcat caccagcggc
     7621 tcccgatagt cgtcctccag cgccaggatg gcccggcgga cgtgcaacgt ctcctggtcc
     7681 tcggccacgc cgagctccgg cgactcgctg cccaccaggt cgtccaccgg caccgtctcg
     7741 aaccgccgcc gttcgtacag ccgcgcgtgc tcgcgccgca cgatcgtgag cagccacggc
     7801 ttggccgccg cctcggtctc cagggcttgc cgtgatctcc aggccctcag cagcgcctcc
     7861 tgcaccacgt cctccgcaac gtcccggtcg cgcgccagcc agtaggcgaa ccggtagagg
     7921 tccgggcgca gctcgtcgca gagccgcgcg aatgcggatt cccctcgctt tccggcaaac
     7981 ggcgcgttca tgtataagga ccgggcgaca agcggcgttg tttcagcttg gcggcacgtg
     8041 cgtgcctggg cccaccgctg gcccgaacca tagccgcgcg gtggcgttcg cgcaaacacg
     8101 cccgtccgac acggagttcg acgatgagtc atcacaccca ctcccagtat ccgcgggtcc
     8161 gcatgcgtcg catgcgccgg gacgggttct cccgcaatct aatgcgggaa acgacggtga
     8221 cagcgtctga tttcatctac cccgtgttcg tgatggaggg agcggcgcag cgggtgcctg
     8281 taccttccat gcccggcgtc gagcgcatga cggtggacga gctggtgcgc gaggccgagg
     8341 ccgtcgcgaa actgggcgtc ccggccgttg cgctcttccc ggtgacggcg tcggaagcca
     8401 agtcgctcga cgggcgcgaa gcctggaatc cggacggcct ggcccagcgc gcggtgcgag
     8461 ccctcaagaa ggcggtccct ggcctgggcg tgatcaccga cgtcgccctg gacccgttca
     8521 cgacgcacgg ccaggacggc atcatcgacg agtcgggcta cgtcgtgaac gacgtgacgg
     8581 tcgaggcgct ggtcaagcag gcgctgtcgc acgccgcggc cggcgccgac gtggttgccc
     8641 cgtcggacat gatggacggt cgcatcggcg cgatccgcga cgcgctcgag gcggcgggcc
     8701 acatccacac ccgcatcctc gcgtattccg ccaagtacgc atcgagtttc tatgggccgt
     8761 tccgcgatgc cgtcggctcg gccggaaacc tcggcaaggg caacaagtac acctaccaga
     8821 tggacccggg caactccgac gaggcgctgc gcgaggtcgc gctcgacctc gaggaaggcg
     8881 ccgaca
//
LOCUS       DAOMFD010000081         8802 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2448_length_8802_cov_33.240311, whole genome shotgun sequence.
ACCESSION   DAOMFD010000081 DAOMFD010000000
VERSION     DAOMFD010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8802
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2448_length_8802_cov_33.240311"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="PL152_06260"
     CDS             <1..779
                     /locus_tag="PL152_06260"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR48919.1"
                     /translation="VGKAIIGRMAGGAYPRPCIAEMGGKNACIVTRRANLDHATQGIV
                     RSAFGMGGQKCSATSRLYVEDGVADELIEKLTRATAAIRIGDPLRRENWLGPVVNAAA
                     YSSYARYVEELRAGGARLLAGGRQLREGALERGYYVEPVLAEAPLAHRLWRHEMFLPI
                     LLVHRVKDREEAMALANDSDLGLTAGFYGAADEVPWFQEHIEAGVTYANRPQGATTGA
                     WPGYQPFGGWKGSGSSGKAIASFYYLAQYLREQSRTVVES"
     gene            779..1624
                     /locus_tag="PL152_06265"
     CDS             779..1624
                     /locus_tag="PL152_06265"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQR48920.1"
                     /translation="MRFLTLSEAIAGHVADGACVALEGFTHLIPFAAGHEIIRQRRRN
                     LHLVRMTPDLVYDQLIGMGCASRLTFSWGGNPGVGSLHRLRDAVENAWPGPLELDEHT
                     HAGMAAAYCAGAARLPLGVLRGYIGTDLVAANPRIRSVECPFTGERLAAVPAINPDVT
                     VLHAQRADRKGNVGLVGIVGAQREAALAARALIVTVEEIVDELPPAMNSIVLPHWIVS
                     AVVLCPGGAYPAYAQGYYGRDNAFYQRWDRIARARDSFSDWMDKHVLGTGDHRGFLAT
                     LRGGA"
     gene            1621..2406
                     /locus_tag="PL152_06270"
     CDS             1621..2406
                     /locus_tag="PL152_06270"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HQR48921.1"
                     /translation="MSDCTRDEMMTVAAARQLWDGCVCFVGIGLPSAACNLARLTHAP
                     DIVLIYESGTIGTRPQVLPLSIGDGELAETASCVVPLPEIFSYYLQGGRVDVGFLGAA
                     QIDRYGNLNSTVIGPYGHPATRLPGAGGAPEIAAHARRTYVVLKATPRSFVERLDFRT
                     SAGFLEGGESRERSGARGGGPQSIITDFGLLAPHAETRELELVALFPGATVEEARAAV
                     GWPLKVSAKVERLDPPTGLELEALRDLHARTREAHSRSVQLPA"
     gene            2436..3764
                     /locus_tag="PL152_06275"
     CDS             2436..3764
                     /locus_tag="PL152_06275"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HQR48922.1"
                     /translation="MQTRHIKTDLPGPKARAILERDASVVSPSYPRDYPFVMSHGRGT
                     EVWDVDGNRFLDFASGIAVCATGHAHPKVVEAVKGAAEKFLHISSDYWHENFTGLAER
                     LSQIAPVGEPGMSFICQSGTEAVEGALKLARYVTGRPRFIGFLGGFHGRTMGSLSFTS
                     SKYTQQKGFAPTMPGVTHVPYPNTYRPLLSGADQGKAVLDYIRMLFERNVPPSEVAAI
                     LVEPIQGEGGYLVPPDGFLAGLRALCDEHGILLICDEVQSGIGRTGRMFAVEHWGVAP
                     DIVTSAKGLGSGLPIGAVIARRSVMEKWGRGAHGNTYGGNPVTCAAAIATIDLVRGEY
                     MANAARMGEHFMGRLRELARDYPCIGDIRGKGLMIGMELIEADAQRAPARALIDAVVR
                     RGYHNGIILLSCGVSTLRFMPPLSVSAAEIDEAVEILRKSLDEALAGTGA"
     gene            3761..6301
                     /locus_tag="PL152_06280"
     CDS             3761..6301
                     /locus_tag="PL152_06280"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HQR48923.1"
                     /translation="MRASHWLIAAVLAAVAGPPALADREPVLKQVDLPHSYYWRELYL
                     PQLTTGPSSAAFTPDGERVVYSMEGSLWEQPMSASEAVELTHARGAYDYQPDVSRDGR
                     YVVFARYDGASIELWRLELASGRELQLTAGGDVNVEPRLSPDGRRLAWVSTQGTGHFN
                     LFIADIDVAGLHGAHPLLGERRSEIDRYYYSAADHAVNPSWSADGATIYYVSNPEVAW
                     GSGDLWAVPVADPTARRMVLHEETTWSAHPEAAPSGPRLLYSSYRGRQWHQLWLTTTD
                     GAAPLPLTFGDYDNRNARWSPDGTRVLYVTNEGGNTSLVVQDVVGGARHEVVATQRRY
                     KERRGTLTLDIVDAAGRRALARVSVVAADGRAYAPRAAWLHGDDGFDRARQAQETHYF
                     HCAPPCRMEVPAGKVSVTVQHGFSVVPWQGSVDVQPGHEASVRAQLVTRELPADFGGW
                     VSADLHVHMNYGGHYRNTPAHLAEQARAEDLDMVFNTIVNKEERIPDIGYFRPGTDPA
                     STRDVVIMQAQEFHTSFWGHLGLLYLADHYLTPDFSAYRHTALASPYPYNGVVADLAH
                     QQGGLVGYVHPYDFPVVPDKEKSLSHELPADVMAGKVDYVEIVAFSDHKATAEVWYHL
                     LNLGFRLPAGAGTDAMANYASLRGPVGMNRVFLDTGGSLDPRAAYTALKEGRTFASNG
                     PLLGLEVGGVKPGGTAKAGTNGARHFRVALRSPVAVDHLELVQNGKVVRAFTMSDDRR
                     TFDGEGDLDIAADGWLVLRAWNDGADPLVLDIYPYATTSPVYLEGFGPRPDSRPDAEY
                     FVRWLDRVIEAARSRSADFNDERERQDTFEYLERARAAYAERAQGRAQ"
     gene            complement(6334..7491)
                     /locus_tag="PL152_06285"
     CDS             complement(6334..7491)
                     /locus_tag="PL152_06285"
                     /inference="COORDINATES: protein motif:HMM:NF015535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HQR48924.1"
                     /translation="MIRPTLALACLVTCSAAIADGPRARDLGVPFDGSPGPLNAITDV
                     AGVTVGQVTLIEDLPGGKAVRTGITAILPRGRQSLDTAVYAAWFPLNGNGETTGTTWL
                     QESGQLDGPIMLTNTHSVGVVRDAVIAWRVRAGGTDSTGYYWSTPVVAETWDGDLNDI
                     NGFHVRPEHADRALDGATGGSVAEGNVGGGTGMICHEFKCGIGTASRRLETAGHTYTL
                     GALVQANYGTRESLRIAGVPVGQHLPVRAGATNRTAGGDSGSIIVVLATDAPLLPDQL
                     KRLARRPAHALGRMGSYSGNGSGDIFFAFSTANAAALTGKPLVQSESLGNDYLDPLFE
                     AAVQATEESIVNALVAARDMHGNEGHFAPALPHDQLVVLLKRYGRYVPPPR"
     gene            7560..8645
                     /locus_tag="PL152_06290"
     CDS             7560..8645
                     /locus_tag="PL152_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HQR48925.1"
                     /translation="MNADLSVPPAEELHRAHALEARHYVGADMHELDRQAVFARSWQL
                     VAHREQLAEPGDHVVEDVAGTPVLLLRGRDGVLRAFPNVCRHRAGPLAHCSGKGMHNL
                     RCKYHGWLYDQEGRLIGAPEMQDAIDFRTADVRLPPLQVREWQGLVFVALSDAVPDFD
                     EVYGGIARRIAPVDLGTMRYTRRDIFDVACNWKVYVDNFLEGYHLPLVHPGLSRLLDY
                     RAYDTELAHWYSMQHSPLRNADAVYGDGEALYFHVFPNVMLNVMPGRLQSNRVLPLGP
                     DRCRVEFDYFYVQDPGATARIDEDRAFSDAVQHEDMAICEAVQKGLASGFYEAGRLSP
                     KRERGVWHFHERLREAYLAAGINTGRG"
BASE COUNT         1379 a         2954 c         3129 g         1340 t
ORIGIN      
        1 ccgtcggcaa ggcgatcatc ggccgcatgg cgggaggcgc gtatccgcgc ccgtgcatcg
       61 ccgagatggg cggcaagaat gcgtgcatcg tcactcgacg ggccaacctc gatcacgcga
      121 cgcagggcat cgtgcgctcg gcgttcggca tgggcggcca gaagtgctcg gcgacgtcgc
      181 gcctctacgt cgaggacgga gtggcggacg aactgatcga gaagctcacg cgcgccaccg
      241 ccgccattcg catcggcgat cccctgaggc gcgagaactg gctgggaccg gtggtgaatg
      301 cggccgcgta ctcgagttac gcgcgttacg tcgaggaact gcgcgcgggc ggcgcgcggc
      361 tgctggcggg tggccggcaa ctcagggaag gtgcgctcga gcgcggctac tacgtggagc
      421 ccgtgctcgc cgaggcgccg ctcgcgcacc ggctgtggcg gcacgagatg ttcctgccga
      481 tcctcctggt gcatcgcgtc aaggaccggg aggaggcgat ggcgctggcg aacgactcgg
      541 acctcgggct caccgccggc ttctacggtg cagccgacga ggtgccttgg ttccaggagc
      601 acatcgaggc gggtgtcacg tacgcgaatc gcccgcaggg cgcgaccacg ggggcgtggc
      661 cgggttatca gccgttcggg ggctggaagg ggtccgggtc ctcgggcaag gcgatcgcct
      721 cgttctatta cctggcgcag tacctgcgcg agcagtcgcg cacggtggtg gagagctgat
      781 gcggttcctc acgctgtccg aagcgattgc cgggcacgtg gcggacgggg cgtgcgtcgc
      841 gctcgagggc ttcacgcacc tgattccgtt cgcagcaggt cacgagatca tccggcagcg
      901 ccggcgcaac ctgcacctcg tgcgcatgac cccggacctc gtctacgacc agttgatcgg
      961 catggggtgc gcgagccgcc tcacgttctc ctggggcggc aatccaggcg tgggctcgct
     1021 gcatcgattg cgggacgccg tggagaacgc ctggcccggc ccgctcgagc tggacgagca
     1081 cacgcatgcc ggcatggcgg cggcgtactg cgcgggcgcc gcgcgcctcc cgttaggtgt
     1141 actgcgtggc tacatcggga cggacctcgt ggccgcgaac ccgcgcatcc gcagcgtcga
     1201 atgcccgttc accggcgagc gactggccgc ggtgccggcg atcaacccgg acgtcacggt
     1261 actgcacgcg cagcgtgccg accgcaaggg caacgtcggg ctcgtcggga tcgtcggcgc
     1321 gcagcgcgag gcggcgctgg cggcgcgcgc gctgatcgtg acggtcgagg agatcgtcga
     1381 cgagttgccg ccggcgatga attcgatcgt cctgccgcac tggatcgtga gcgccgtcgt
     1441 gctctgcccg ggcggtgcgt accccgccta tgcccagggc tactacggcc gcgacaacgc
     1501 cttctaccag cgctgggaca ggattgcgcg cgcgcgtgat tcgttcagcg actggatgga
     1561 caagcacgtg ctgggcacag gagaccatcg cggattcctt gcgaccctca gggggggggc
     1621 atgagcgact gcacgcgcga cgaaatgatg accgtggccg cggcacggca gctctgggac
     1681 gggtgcgtgt gcttcgtcgg catcgggctg ccgagcgccg cctgcaacct ggcgcggctc
     1741 acgcacgcgc ccgacatcgt gctcatctac gagtccggaa ccatcgggac gcgcccgcag
     1801 gttctgccgc tgtcgatcgg cgatggcgaa ctcgccgaga ccgcgagctg cgtggtcccg
     1861 ctgcccgaga tcttcagcta ttacctgcag ggcgggcgcg tggacgtcgg cttcctgggt
     1921 gcggcacaga tcgaccgcta cggcaacctc aacagcaccg tgatcggtcc ctacgggcac
     1981 ccggcgacgc gattgccggg tgcgggcggg gcgccggaga tcgccgcgca cgcccggcgg
     2041 acctacgtcg tgctgaaggc gacaccgcgc agcttcgtcg agcggctcga cttcaggacc
     2101 agcgcgggct tcctcgaggg cggcgagtcg cgcgagcgca gcggcgcgcg cggcggcggg
     2161 ccgcagtcca tcatcaccga cttcggtctg ctcgcgccgc acgcggagac gagggagctc
     2221 gaactcgtcg cgctgtttcc aggagcgacg gtcgaggaag cgcgcgcggc ggtcggctgg
     2281 ccgctcaagg tgtccgcgaa ggtcgagcgt ctcgacccgc cgaccggact ggagctcgag
     2341 gctttacgcg atctgcatgc gcgtacgagg gaagcacact cgcgttccgt gcaactgccg
     2401 gcgtgagcaa cgaacaacgc tgcaaggaga acatcgtgca aaccaggcac atcaagaccg
     2461 acctgccggg tcccaaggcc cgcgccatcc tcgagcgaga cgcctcagtc gtgtcgccct
     2521 cgtatccccg cgactacccg ttcgtcatgt cgcacggacg cggcaccgaa gtctgggacg
     2581 tggacggcaa ccgcttcctc gatttcgcgt cgggcatcgc ggtgtgcgcc acgggtcacg
     2641 cgcatccaaa ggtcgtcgag gccgtcaagg gcgcggccga gaagttcctg cacatctcga
     2701 gcgactactg gcacgagaac ttcaccggcc tggccgaacg cctgtcgcag atcgcgccgg
     2761 tcggcgagcc gggcatgagc ttcatctgcc agtcgggaac cgaggcggtc gagggtgcgc
     2821 tgaagctcgc gcgctacgtg accggcaggc cgcgattcat cgggttcctc ggcgggttcc
     2881 acgggcgcac gatgggctcg ctgtcgttca cctcgagcaa gtacacgcag cagaagggct
     2941 tcgcgccgac gatgcccggc gtgacacacg tgccgtaccc gaacacgtac cggccgctac
     3001 tgagcggtgc ggaccagggc aaggcggtgc tcgactacat ccgcatgctg ttcgagcgca
     3061 acgtgccgcc gtccgaggtc gcggccatcc tcgtcgagcc gatccagggc gagggcggtt
     3121 acctcgtgcc gcccgacggg ttcctcgccg gcctgcgcgc gctctgcgac gagcacggca
     3181 tcctgctcat ctgcgacgaa gtgcagtcgg gcatcgggcg caccggccgc atgttcgccg
     3241 tcgagcactg gggcgtcgcg ccggacatcg tcacgagcgc gaagggcctg ggctccggac
     3301 tgccgatcgg agcggtgatc gccaggcgct cggtgatgga gaagtggggt cgcggcgcgc
     3361 acggcaacac ctacggcggc aacccggtga cctgcgcggc ggcaatcgcg accatcgatc
     3421 tcgtgcgggg cgaatacatg gccaatgccg cgcgcatggg cgagcacttc atgggccggc
     3481 tgcgcgaact ggcgcgcgac tacccgtgca tcggcgacat ccgcggcaag ggcctgatga
     3541 tcggcatgga actcatcgag gcagacgcgc agcgtgcgcc tgcgcgagca ctgatcgacg
     3601 cggtggtccg gcgcggttac cacaacggga tcatcctgct gtcgtgcggc gtgagcacgc
     3661 tgcgattcat gccgccgctg agcgtcagcg ccgcggagat cgacgaggcc gtggagatcc
     3721 tgcgcaagag cctggacgag gcgctggcgg ggaccggggc ttgagggcgt cgcactggct
     3781 gatcgcggcg gtgctggccg ccgtggccgg tccgccggcg ctggcggacc gggagcccgt
     3841 gctgaagcag gtggacctgc cgcacagcta ctactggcgc gagctctacc ttccgcagct
     3901 cacgaccggg ccgtcgtccg ccgccttcac accggacggc gagcgcgtcg tgtacagcat
     3961 ggaagggtcg ctgtgggaac agccgatgtc ggcgagcgaa gccgtcgaac tcacgcacgc
     4021 gcgaggcgct tacgactacc agccggacgt gtcgcgcgac ggacggtatg tcgtgttcgc
     4081 ccgctacgac ggcgcgtcga tcgaattgtg gcgcctcgaa ctcgcgagcg ggcgggaact
     4141 gcagctgacc gccggcggcg acgtgaacgt ggagccgcgc ctgtcccccg acggccgccg
     4201 cctcgcatgg gtgtcgacac aggggacggg tcacttcaac ctgttcattg ccgacatcga
     4261 cgtcgcgggc ctgcacgggg cgcatcctct gctgggcgaa cggcgcagcg agatcgatcg
     4321 ttactactac tcggccgctg accacgccgt gaacccgtcg tggtcggcgg acggtgcgac
     4381 catctactac gtgagcaatc ccgaggtcgc ctggggctcc ggggacttgt gggccgtccc
     4441 ggtggccgat ccgaccgccc gccgcatggt gctgcacgag gagacgacct ggagcgcgca
     4501 cccggaagcg gctccgtcag ggccgcgcct gctgtacagc agctaccgcg gtcgccagtg
     4561 gcaccagcta tggctcacca cgaccgatgg agcggcgccg ctgccgctca ccttcggcga
     4621 ctacgacaac cgcaatgcgc gctggtcacc cgacggcacg cgcgtcctgt acgtcacgaa
     4681 cgagggcggc aacacgtcgc tggtcgtcca ggacgtcgtg ggaggggcgc ggcacgaagt
     4741 ggttgcgacg cagcgccggt acaaggagcg gcgtggaacc ctgaccctgg acattgtcga
     4801 cgccgcgggt cgcagggccc tggcgcgcgt ctcggtggtc gcggcggatg gtcgcgccta
     4861 cgcgccgcgc gccgcctggc tgcacggtga cgacggcttc gatcgtgccc gacaggcaca
     4921 ggaaactcac tatttccatt gcgcgccccc gtgcaggatg gaagtgccgg cgggaaaggt
     4981 gtcggtgacc gtccaacacg gcttctccgt ggtgccatgg cagggctcgg tggacgtcca
     5041 acccggccac gaggcgagcg tgcgcgcgca actcgtgacc cgggagctgc ctgccgactt
     5101 cggcggctgg gtcagcgccg acctgcacgt gcacatgaac tacggcggcc actaccgcaa
     5161 tacgccggcc cacctggccg agcaggcgcg ggcggaagac ctcgacatgg tgttcaacac
     5221 gatcgtgaac aaggaggagc ggattccgga catcgggtat ttccgccccg gcaccgaccc
     5281 ggcgagcacg cgcgacgtgg tgatcatgca ggcccaggaa ttccacacga gtttctgggg
     5341 tcacctgggc ctgctgtacc tggcggatca ctatctcacg ccggatttct cggcgtaccg
     5401 ccacactgcg ctggcgagcc cgtacccgta caacggcgtg gtggctgacc tggcccacca
     5461 gcagggcggg ctcgtgggct acgtgcatcc ctacgacttc ccggtcgtgc cggacaagga
     5521 aaagtccctg agccatgagc tgcccgctga cgtgatggcc ggcaaggtgg actacgtcga
     5581 gatcgtcgca ttctcggacc acaaggcgac ggccgaggtc tggtatcacc tgctcaatct
     5641 cggcttccgg ttgccggccg gggccggcac ggacgcgatg gccaattacg cctcgctgcg
     5701 cgggcccgtg ggcatgaatc gggtgttcct cgacacggga ggcagcctcg atccgcgcgc
     5761 cgcatacacc gcgctcaagg aaggccggac gtttgcatcg aacgggccgc tgctcggcct
     5821 cgaggtgggc ggcgtgaagc cgggcggaac tgcgaaagca ggcacgaacg gcgcaaggca
     5881 tttccgcgtg gcattgcgct caccggtcgc cgtggaccac ctcgagctcg tgcagaacgg
     5941 caaggtggtc cgggcgttca ccatgtcgga cgaccgccgc accttcgacg gcgaaggcga
     6001 cctcgacatc gccgccgacg gttggctcgt cctgcgcgcc tggaacgacg gggccgatcc
     6061 gctggtgctc gacatctacc cttacgcgac cacgagcccg gtctacctcg aaggcttcgg
     6121 tccgaggcca gactcgcgac cggacgccga atatttcgtc cgttggctcg atcgggtcat
     6181 cgaggcggcg cgctcccgca gtgccgattt caacgacgag cgcgaacgac aggacacgtt
     6241 cgagtacctc gagcgggcgc gcgcggctta cgccgagcgc gcgcagggca gggcgcagta
     6301 gacatcgagc gagcgagcgg catctggcga tcttcagcgc ggcggcggca cgtagcgtcc
     6361 gtagcgcttg agcagcacca ccagttgatc gtgcggcagg gcgggcgcga agtgaccctc
     6421 gttgccatgc atgtcgcgcg cggcaaccag cgcgttgacg atggattcct cggttgcctg
     6481 taccgccgcc tcgaacagtg gatccaggta gtcgttgccc agcgactcgg actgcaccag
     6541 cggcttaccg gtcagcgccg cggcattcgc tgtcgagaag gcgaagaaga tgtcgcctga
     6601 gccattgccc gaatagctgc ccatgcggcc cagcgcgtgc gccgggcgcc tcgcgaggcg
     6661 cttcagctgg tccggcagga gcggcgcgtc ggtcgcgagc acgacgatga tcgagccgga
     6721 atcaccacct gcagtccggt tcgtggcacc ggcccgcacc ggcaggtgtt gcccgaccgg
     6781 cacgcccgca atgcgcaacg actcccgtgt gccgtagttg gcctgcacca gcgcgcccag
     6841 cgtatacgtg tgcccggcgg tctcgagtcg gcgcgaggcc gtgccgatcc cgcacttgaa
     6901 ctcgtggcag atcatcccgg tgccgccgcc gacgttgcct tccgcgacgg atccgccggt
     6961 cgcgccatcg agcgcgcggt cggcgtgttc cggacgaacg tggaagccgt tgatgtcgtt
     7021 caggtcgccg tcccaggtct ctgcgacgac cggcgtcgac cagtagtacc cggtcgagtc
     7081 ggttccaccc gcgcggaccc gccacgcgat gaccgcgtcg cgcaccacgc cgacgctgtg
     7141 cgtgttggtc agcatgatcg ggccgtcgag ctgcccggac tcctgcagcc aggtcgttcc
     7201 ggtggtctcg ccattgccgt tcagcggaaa ccaggccgca tagaccgccg tgtccaggga
     7261 ctggcggccg cgcggcagga tcgccgtgat gccggtccgc accgccttgc cgcccggcag
     7321 gtcctcgatg agcgtgacct ggccgacggt caccccggcg acatccgtga tggcattgag
     7381 tggcccagga ctcccgtcga atggcacgcc gaggtcgcgg gcacgcggcc cgtccgcaat
     7441 tgctgcgctg caggtgacga ggcaggcgag cgcgagcgtc ggtcggatca tgggtggctc
     7501 cggcagcggg ttatcgccga caatgtctgc ccgcgagctt agcgaaggaa cctgctgcga
     7561 tgaatgccga cctgagcgtc ccgccggccg aagagctgca cagggcccac gcgctcgagg
     7621 cgcgccacta cgtcggcgcc gacatgcacg agctggaccg gcaggcagta tttgcgcgtt
     7681 cgtggcagct cgtggcgcac cgggaacaac tcgcggagcc gggcgaccac gtggtcgagg
     7741 acgtcgccgg gacgccggtg ctgctgttgc gcggtcgcga cggcgtattg cgggcgttcc
     7801 ccaacgtctg ccggcatcgg gccgggccgc tcgcccactg cagcggcaag ggcatgcaca
     7861 acctgcgctg caagtatcac ggctggctgt acgaccagga gggccgcctg atcggcgccc
     7921 cggaaatgca ggacgcgatc gacttccgga cggcggacgt gcggctgccg ccgctgcagg
     7981 tgcgcgagtg gcaggggctg gtcttcgtcg cgctgagcga cgccgtgccc gatttcgacg
     8041 aggtgtacgg ggggatcgcc cggcgcatcg caccggtgga cctcggcacg atgcgctaca
     8101 cgcggcgcga catcttcgac gtcgcgtgca actggaaggt gtacgtggac aactttctcg
     8161 agggctatca cctgccgctc gtccacccgg gtctctcgcg cctgctggac taccgcgcct
     8221 acgacaccga gctggcgcac tggtactcga tgcagcactc gccgctgcgc aatgccgacg
     8281 cggtctatgg cgacggcgag gcgctctatt tccacgtgtt tcccaacgtc atgctgaacg
     8341 tgatgcccgg ccggctgcag tccaaccgcg tgctgccgct cgggccggac cgctgtcggg
     8401 tcgagttcga ctacttctac gtgcaggacc cgggcgccac ggctcggatc gacgaggacc
     8461 gcgcgttcag cgacgccgtc cagcacgagg acatggcgat ctgcgaggcc gtgcagaagg
     8521 gcctcgcgtc cgggttctac gaggccgggc ggctgagccc gaagcgcgag cgcggcgtct
     8581 ggcacttcca cgagcggctg cgcgaggcgt acctggcagc cgggatcaac accggccgcg
     8641 gctgaatcga ccgacgatat tattctggag tctgggggga tcgatgaacg accggcatgc
     8701 gaccggagcc gcggtggggc gcgagctggg cttctggatg tgcacggcgc tggtggtcgg
     8761 caacaccatc ggcatgggga tcttcctgct gccggcgtcg ct
//
LOCUS       DAOMFD010000082         8710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2490_length_8710_cov_40.091161, whole genome shotgun sequence.
ACCESSION   DAOMFD010000082 DAOMFD010000000
VERSION     DAOMFD010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2490_length_8710_cov_40.091161"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1655)
                     /locus_tag="PL152_06295"
     CDS             complement(<1..1655)
                     /locus_tag="PL152_06295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008332965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HQR48926.1"
                     /translation="MTMLRTERVREFRRLLGERILVLDGAMGTVIQSYRLGEQDYRGD
                     RFSRWPRDLKGNNDLLSLTRPEVIGEIHRRYLEAGADIVETNTFNSNAQSQADYGTES
                     LVGELNLAAAQIARRAADLVAARTGRPRFVAGALGPTNRTASLSPDVNDPGFRNVTFD
                     ALAASYAEATRALVEGGVDLLLVETIFDTLNAKAALYAVRQVLDELGLDLPIMVSGTI
                     TDASGRTLSGQTVEAFWNSVRHARPEVVGLNCALGAKQLRPYVEELSTLADACVCAYP
                     NAGLPNAFGEYDETACETADLIRDYAASGFVNIVGGCCGTTPEHIAHVAEAVAGLPPR
                     RPAVLEPRCRLSGLEPLNIGPESLFVNVGERTNVTGSAKFRRLIEAEDYAGALDVARQ
                     QVASGAQVIDVNMDEGMLDSEGAMVRFLNMLAGEPDVARVPVMIDSSKWSVIEAGLKC
                     LQGKGIVNSISLKEGERAFLEQARRVRAYGAAVVVMAFDEQGQADTVERKVSICRRSY
                     QLLTKQAGFPPEDIIFDPNIFAVATGIEAHDRYALDFIEATAR"
     gene            complement(1652..2575)
                     /gene="metF"
                     /locus_tag="PL152_06300"
     CDS             complement(1652..2575)
                     /gene="metF"
                     /locus_tag="PL152_06300"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006950.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HQR48927.1"
                     /translation="MEAGTMIHGANALAAGAQRPVLVSFEFFPPNDPEMDRTLWASIQ
                     RLAPLAPRFVSVTYGADGSTRERTHNVVTRILRETALTPAPHLTCVGAKREEILAIAR
                     AYWEQGVRHIVALRGDPPAGQGAYRPEPDGFPYAEDLVRGLRSVADFEISVAAYPETH
                     PEAPSAEFDLDRLAAKVDAGARRAITQFFFDTDAFLRFRDRCAARRIEAELVPGILPI
                     TRFPQMLRFAERCGASVPGWLRQRFDGLDDDPETRRMIAASVAIEQVHRLRQHGVQEF
                     HFYTLNRAELTYAICHALGVRPAAVTRAEAT"
     gene            complement(2602..3501)
                     /locus_tag="PL152_06305"
     CDS             complement(2602..3501)
                     /locus_tag="PL152_06305"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR48928.1"
                     /translation="MLRGLRAAAEPSRLRLLGVLRLGEFTVSELTAILGQSQPRVSRH
                     LKLLSDGGLLERFREQHWVFHRVPADGEGAELVRALIDLLDRDDPLVALDRERAAAVL
                     AERARAVADDGGAPDPHAPRGSAASDLAQVIAAELGERGFDSVLYAGPAPAEMLRVIG
                     PRSRRVLGLSESRGEVQRARATLHGNGLAHCELQCGDLPGAALDGASFDAVVVDRVLA
                     RSARRGEVLREAARVLRSAGKLVIVEDYESLAEAGGGNPLATVRGWLADAGLVCERLR
                     PVDTGATHVLLATAGRDRAERAA"
     gene            3761..4384
                     /locus_tag="PL152_06310"
     CDS             3761..4384
                     /locus_tag="PL152_06310"
                     /inference="COORDINATES: protein motif:HMM:NF013341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HQR48929.1"
                     /translation="MSEGRWANAVLFRYERGGESWVVKDFAPRAFLVRNLIGRFLVRR
                     ELAGLRRVQGVAIAPRDAFRVDAHALAYRFVPGVSLRLTARSAVGGEFFRALEQGLRQ
                     VHSSARLVHLDLRNAHNILVTDQGEPSLLDFQSWVGTRWLPSWLRRFAEQIDLAAIYK
                     HWAKRSPETLGDERADALASMNRVRPLWVLRGYIGAGNPGRKRRNRP"
     gene            4417..4980
                     /locus_tag="PL152_06315"
     CDS             4417..4980
                     /locus_tag="PL152_06315"
                     /inference="COORDINATES: protein motif:HMM:NF013394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HQR48930.1"
                     /translation="MLEAIHRLFNHPALRKALVVARVPLAIAAVAGLLWVMDPTWLVP
                     GFAVSMFGEAIQLWCFASLDKGRTLAFNGPYAFVRNPMYLGRYFILLGGLMLLGRGWV
                     LVLFTAVYYFYMVNRVGREEDYLRKPLGEPYEDYLRTVNRFMPGSPKPGSRVAFWDWK
                     LLKRNHGTTNLVATLAFWVLAWVVALR"
     gene            complement(5039..5956)
                     /locus_tag="PL152_06320"
     CDS             complement(5039..5956)
                     /locus_tag="PL152_06320"
                     /inference="COORDINATES: protein motif:HMM:NF013589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HQR48931.1"
                     /translation="MAEKDSLRRFVFERYPFRGQLVHLGPAWRAMMEHHDYPDAVRDS
                     LGEAVAAAALLASTLKFKGQLSLQLRGTGPMHLMLVECSDGLAMRAVARFRLPPATRD
                     LRELSGNGTLTVTLEGEDLVNRYQGVVPLAGERMADCLREYFETSEQLPTRLWLHADG
                     ERSSGLLLQRLPVSSRPASATESGKGGVAEDEIDDAWQRVQLVAGTVTGPELAGLADR
                     ELLRRLFAEDDVRMFESAPVFFRCRCSRERVAAMLRSLGREEIDSILEERGDVEVRCE
                     FCSRSYRFDAVDAGTIFSAVPDAPRGGVH"
     gene            complement(5959..7488)
                     /locus_tag="PL152_06325"
     CDS             complement(5959..7488)
                     /locus_tag="PL152_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HQR48932.1"
                     /translation="MTVIREDDFVQSVADALQFISYYHPVDYLEHLGRAYEREQSPAA
                     KDAIAQILTNSRMCAEGHRPICQDTGIVNMFLKVGMDVRWETRRSLAEMVNEGVHRAY
                     EFPDNRLRASVVDDPVFARKNTRDNTPAVFHVELVPGDTVEVTVAAKGGGSENKSKFV
                     MLNPSDSIEEWVVKTVPTMGAGWCPPGMLGIGIGGTAEKAMLLAKEALMDPIDMHELI
                     ARGPANKLEELRISLYEKVNALGIGAQGLGGLTTVLDVKIRTHATHAASKPVAMIPNC
                     AATRHAHFVLDGSGPAVLEPPSLEHWPQVTWQAGAGSRRVNLDTLTRAEVQAWKPGET
                     LLLNGKMLTGRDAAHKRIADLFASGQGLPAGVDFTNRVIYYVGPVDPVRDEVVGPAGP
                     TTATRMDKFTDMMLEKTGLIAMIGKSERGPVAIESIRKHKAAYLMAVGGAAYLVSKAI
                     RAARVVAFGDLGMEAIYEFEVKDMPVTVAVDSSGVSVHHTAPAAWAEKIRTLAIPVRV
                     A"
     gene            complement(7499..>8710)
                     /gene="gatB"
                     /locus_tag="PL152_06330"
     CDS             complement(7499..>8710)
                     /gene="gatB"
                     /locus_tag="PL152_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HQR48933.1"
                     /translation="FARKNYFYPDLPKGYQISQYELPVVQRGTLEIVLEDGTVKRVGI
                     TRAHLEEDAGKSLHEDFHGLSGIDLNRAGTPLLEIVSEPDMRSAKEAVAYMKKIHTLV
                     RYLGICDGNMQEGSFRCDANVSVRPRGQEKFGTRAEIKNLNSFRFVERAINYEVERQV
                     DLLEGGGTVVQETRLYDPDRGETRSMRSKEEANDYRYFPDPDLLPLAIDEALIESVRA
                     ALPELPDEKAARFVRDHGLSAYDAGVLTASRELADYYEAVVQGLGGQHKLAANWVMGD
                     LSGALNRENLEVGQSRIGASTLAQLLARIVDATISGKIAKEVFEAMWTEGRGADEIID
                     ARGLRQITDTSAIERAIDEVMARSPQQLAEYRSGKDKLFGYFVGQVMKATQGKANPAQ
                     LNELLKRKLSG"
BASE COUNT         1273 a         3076 c         2949 g         1412 t
ORIGIN      
        1 atgcgcgccg tggcctcgat gaagtcgagc gcatatcgat cgtgcgcctc gatgccggtc
       61 gccaccgcga agatgttcgg gtcgaagatg atgtcctcgg gcgggaaccc ggcctgcttc
      121 gtcagcagct ggtaggaacg ccggcagatg gagaccttgc gctcgacggt gtcggcctgg
      181 ccctgctcgt cgaacgccat cacgacgacc gccgcgccgt acgcgcgcac tcgccgcgcc
      241 tgctcgagga acgcgcgctc gccttccttg aggctgatcg agttgacgat gcccttgccc
      301 tgcaggcact tgaggccggc ctcgatgacc gaccacttcg acgagtcgat catgaccggg
      361 acgcgcgcca cgtccggctc gccggcgagc atgttgagga agcgcaccat cgcgccttcc
      421 gaatcgagca tgccctcgtc catgttgacg tcgatgacct gggcgccgct cgcgacctgt
      481 tgccgcgcga catccagcgc gccggcgtag tcctccgctt cgatgaggcg gcggaatttc
      541 gccgacccgg tgacgttggt gcgctcgccg acgttgacga agagactctc cggcccgatg
      601 ttcagcggct cgagcccgct caggcggcag cgaggctcga gcacggccgg gcggcgtggc
      661 ggcagcccgg cgacggcttc cgcgacgtgc gcgatgtgct cgggtgtcgt cccgcagcag
      721 ccgccgacga tgttcacgaa gccgctcgcc gcgtaatcgc ggatcaggtc ggcggtttcg
      781 cacgcggtct catcgtattc cccgaaggca ttcggcagcc ccgcgttcgg gtacgcgcag
      841 acacaggcgt cggccagggt cgacagctcc tcgacgtagg gccgcagctg cttcgcgccg
      901 agcgcgcagt tgaggccgac gacctcgggg cgcgcgtgcc gcaccgagtt ccagaacgcc
      961 tcgacggtct gcccggacag cgtgcggccc gacgcgtcgg tgatcgtgcc cgacaccatg
     1021 atcggcaggt cgaggccaag ctcgtccagt acctggcgca cggcgtagag cgcggccttg
     1081 gcattcagcg tgtcgaagat cgtctcgacg agcagcaggt ccacgcctcc ttcgacgagg
     1141 gcgcgcgttg cttcggcgta gctcgcggcc agcgcatcga acgtgacgtt gcggaagccc
     1201 gggtcattga cgtcgggcga gagcgaagcc gtccggttcg tcgggccgag cgccccggcc
     1261 acgaagcgcg ggcggcccgt gcgggccgcg acgaggtcgg ccgcgcgtcg cgcgatctgc
     1321 gcggctgcga ggttcagctc gcccaccagc gactcggtac cgtagtccgc ctgcgactgc
     1381 gcgttggaat tgaaggtgtt ggtctcgacg atgtcggccc ctgcttccag gtatcggcga
     1441 tgaatctcgc cgatcacctc ggggcgcgtg agcgacaaca ggtcgttgtt gcccttgagg
     1501 tcgcgcggcc agcggctgaa ccggtcgcca cggtaatcct gctcgcctag ccggtaggac
     1561 tggatcaccg tacccatcgc gccgtcgagc acgaggatgc gctcgccgag caggcggcgg
     1621 aattcccgga cgcgttcggt gcgcagcatg gtcatgtcgc ctccgccctg gtcaccgccg
     1681 ctggccgcac gccgagcgcg tggcagatcg cgtaggtgag ctcggcgcgg ttgagcgtat
     1741 agaagtggaa ttcctgcacg ccgtgctgcc ggagccggtg cacctgctcg atggcgacgc
     1801 tcgccgcgat catgcggcgc gtctccgggt cgtcgtccag gccgtcgaag cgctgcctga
     1861 gccagcccgg cacgctcgcg ccgcagcgct ccgcgaagcg cagcatctgc gggaatcgcg
     1921 tgatcggcag gatcccgggc accagctccg cctcgatccg gcgcgcggcg cagcgatcgc
     1981 ggaagcgcag gaacgcgtcc gtgtcgaaga agaactgcgt gatggcgcga cgcgcacccg
     2041 cgtcgacctt ggcggccagc cggtcgaggt cgaactcggc gctcggcgcc tccggatgcg
     2101 tctccgggta ggccgccacg gagatctcga agtcggccac tgagcgcagg ccgcggacca
     2161 gatcttcggc ataagggaaa ccgtctggct ccgggcggta ggcgccctgc ccggccggcg
     2221 gatcaccgcg cagggccacg atgtgccgca cgccctgctc ccagtacgcg cgcgcgatgg
     2281 cgaggatctc ctcgcgcttg gccccgacgc aagtcaggtg cggcgccggc gtcagcgcgg
     2341 tctccctgag gatgcgcgtg acgacattgt gcgtgcgctc gcgcgtcgag ccgtcggcgc
     2401 cgtaggtcac cgagacgaaa cgcggggcga gcggcgcgag ccgctggatc gaagcccaca
     2461 acgtgcgatc catctccggg tcgttcggcg ggaagaactc gaacgacacg agcaccgggc
     2521 gctgcgctcc ggccgccagt gcgttcgcac catgaatcat cgtgcctgct tccatacgca
     2581 tgcctcctcg cgcgccggcg ctcacgcggc ccgttctgcc cggtcacgac cagccgttgc
     2641 cagcagcacg tgcgtcgccc ccgtgtcgac ggggcgcagc cgctcgcaga cgagtccagc
     2701 gtccgcgagc cagccgcgga ccgtcgcgag cggattgccg ccgcccgctt ccgccaacga
     2761 ctcgtagtcc tcgacgatca cgagcttgcc ggcggaccgc agcacgcgcg ccgcctctcg
     2821 cagcacctcg ccgcgccggg ccgaccgcgc gagcactcga tcgacgacga ccgcatcgaa
     2881 gctcgcgccg tcgagagcag ctcccgggag atcgccacac tgcaactcgc agtgcgcgag
     2941 tccgttgccg tgcagggtcg cgcgcgcgcg ctggacctcg ccacgagact cgctcaaccc
     3001 cagcacccgc cgcgatcggg gcccgatgac gcgcagcatc tcggccggcg ccggcccggc
     3061 gtagaggacg gaatcgaaac ctcgctcccc cagctccgct gcaatgacct gcgcaaggtc
     3121 gctggccgca ctgccgcgcg gagcgtgcgg gtccggtgcg ccgccgtcat cagcaacggc
     3181 acgtgcgcgc tcggccagga cggccgctgc gcgctcgcgg tccagtgcca ccagcgggtc
     3241 gtcacggtcc agcaaatcga tcaacgcacg gaccagttcg gcgccctcgc cgtcggccgg
     3301 cacgcgatgg aatacccaat gctgttcgcg gaatcgctcg aggagcccgc cgtcgctcag
     3361 gagcttgagg tgcctggaca cccgaggctg gctctggccc aggatcgcgg tcagctcgga
     3421 cacggtgaat tcgccgagcc tcaggacgcc aagcagcctc aggcggctcg gttccgccgc
     3481 ggctcgcagg ccgcgcagca tgctctcatg ggactgggac atttcataca aatataaaca
     3541 agtctttatg tgttttccag tgggcccatc gatcgctggt cgatgcttcg agttccagcg
     3601 cgcaagttgt tatcatccat ggaattgttc gccgtctcgc cccgaccgac ctgccgaccc
     3661 gatgccgacc tcccccagcc cacgcccggt ttcggcgagt tcccagcctg cccgggccac
     3721 gttcagccgg gcggacctcg agcgcgggcc acgccaccgg ttgagcgagg ggcggtgggc
     3781 caatgccgtc ctgtttcgct acgagcgggg cggcgagtcg tgggtggtca aggacttcgc
     3841 gccccgcgcc ttcctcgtcc gcaacctgat cggccgtttc ctggtgcgcc gcgaactcgc
     3901 cgggctcagg cgggtccagg gcgtcgcaat cgcgccgcgc gacgcgttcc gggtcgatgc
     3961 ccatgccctc gcctatcgct tcgtgccggg cgtgagcctg cgcctgaccg cgcgctcggc
     4021 ggtcgggggc gagttcttcc gcgcgctcga gcagggcctc aggcaggtgc actcgagcgc
     4081 gcggctcgtg cacctcgacc tgcgcaatgc gcacaacatc ctggtcacgg accagggcga
     4141 gccgtcgctg ctcgacttcc agtcctgggt cgggacgcgc tggctgccgt cgtggctgcg
     4201 tcggtttgcc gagcaaatcg atcttgccgc gatctacaag cactgggcga agcgcagtcc
     4261 ggaaacgctc ggggacgaac gcgccgatgc gctcgccagc atgaaccgcg ttcgcccgct
     4321 ctgggtgctg cgcggctaca tcggggcggg caaccccggc aggaagcgcc gcaaccgtcc
     4381 ataatggtgc gctgaatcgc gcctgcggag ttcgccgtgc tcgaagccat acatcgcctg
     4441 ttcaaccacc cggccttgcg caaggccctg gtcgtcgcgc gcgtgccgct ggcgatcgcg
     4501 gcggtcgcgg gcctgctctg ggtgatggac ccgacctggc tggtgccggg cttcgcggtg
     4561 tcgatgttcg gcgaggcgat ccagctgtgg tgcttcgcct cgctcgacaa gggacggacg
     4621 ctggcgttca acgggcccta cgcattcgtg cgcaacccga tgtacctcgg tcgctacttc
     4681 atcctgctcg gcgggctgat gctgctaggt cgcgggtggg tgctcgtgct cttcaccgcc
     4741 gtgtactact tctacatggt caaccgggta ggacgcgaag aagactactt gcgcaagccg
     4801 ctaggggaac cgtacgagga ttacctgcgt accgtgaatc gcttcatgcc cggctcgccg
     4861 aagcccggca gccgggtggc gttctgggac tggaagctcc tgaagcgcaa ccacgggacc
     4921 accaacctgg tcgcgacgct cgcgttctgg gtcctcgcgt gggtggtcgc gctgcgctga
     4981 tcgatcgacc gcggcatgcc cgcgggcccg cgccaggccg gtcagtgcac gcgggcagtc
     5041 aatgcacgcc gccgcgcggc gcgtcgggca cggcgctgaa gatcgtgccg gcatccactg
     5101 cgtcgaagcg gtaactgcgg ctgcagaact cgcagcgtac ctcgacgtcg ccgcgttcct
     5161 cgaggatcga gtcgatctcc tcgcgaccga gcgagcgcag catcgcggcg acgcgctcgc
     5221 gcgagcaccg gcaacggaag aacacgggcg ccgactcgaa catccgcacg tcgtcctcgg
     5281 cgaacagtcg ccgcagcagc tcgcggtcgg cgagaccggc gagttccggg cccgtgacgg
     5341 tgcccgccac gagctgcacg cgctgccagg catcgtcgat ctcgtcctcc gcgacgccgc
     5401 ccttgccgga ctccgtcgcc gacgcgggcc gcgacgagac gggcagccgc tgcagcagca
     5461 acccgctcga acgctcgccg tctgcgtgca gccagagccg cgtcggcagc tgctccgacg
     5521 tctcgaaata ctccctgagg cagtcggcca tgcgctcgcc cgcgagcggc accacgccct
     5581 ggtagcgatt gacgaggtcc tcgccctcga gcgtgaccgt gagggtgccg ttgcccgaga
     5641 gttcccgcag gtcgcgcgtc gccggcggca ggcggaaccg cgcgacggcg cgcatcgcga
     5701 ggccgtcgct gcactcgacg agcatcaggt gcatcgggcc ggtgcccctg agctgcaggg
     5761 acagctggcc cttgaacttg agcgtggacg cgagcaaggc cgcggccgcg acggcttctc
     5821 ccaggctgtc gcgcactgca tcggggtaat cgtgatgctc catcatggcc cgccacgccg
     5881 gcccgaggtg cacgagctgc ccgcggaacg ggtagcgctc gaacacgaaa cgccgcagcg
     5941 agtccttctc cgccatgctc aggcgacgcg caccgggatc gcgagcgtgc ggatcttctc
     6001 ggcccacgcg gccggcgcgg tgtggtgcac cgagacgccg ctcgagtcca ccgccacggt
     6061 caccggcatg tccttgacct cgaactcgta gatcgcctcc atgccgaggt cgccgaaggc
     6121 gaccacccgg gcggcccgga tcgccttcga gacgaggtac gcggcgccgc cgaccgccat
     6181 gaggtacgcg gccttgtgct tgcggatgga ttcgatcgcg accggcccgc gctcggactt
     6241 gccgatcatc gcgatcaggc cggtcttctc gagcatcatg tcggtgaact tgtccatgcg
     6301 ggtcgccgtc gtcgggcccg ccggccccac cacctcgtcg cgcaccgggt cgaccgggcc
     6361 gacgtaatag atcacgcgat tggtgaaatc gacgccggca ggcagcccct gcccactggc
     6421 gaacaggtcc gcgatgcgct tgtgcgcggc gtcgcggccg gtgagcatct tcccgttgag
     6481 cagcagcgtc tcgcccggct tccaggcctg gacctccgca cgcgtcaggg tgtcgaggtt
     6541 gacgcggcgc gatccggcgc cggcctgcca cgtgacctgg ggccaatgct cgagcgaggg
     6601 cggctccagc actgcgggcc ccgatccgtc gagcacgaaa tgcgcgtgcc gggtcgcagc
     6661 gcagttcggg atcatcgcca cgggcttgga cgccgcatgc gtggcgtgcg tgcggatctt
     6721 cacgtcgagc acggtggtga gcccgccgag gccctgcgcg ccgatgccga gcgcattgac
     6781 cttctcgtac agcgagatcc gcagctcctc gagcttgttc gccggaccgc gcgcgatgag
     6841 ctcgtgcatg tcgatcgggt ccatgagcgc ctccttggcg agcagcatgg ccttctccgc
     6901 cgtgccgccg atgccgatgc ccagcatgcc gggcgggcac cagccggcgc ccatcgtcgg
     6961 caccgtcttc acgacccact cctcgatcga gtcgctgggg ttcagcatca cgaacttcga
     7021 cttgttctcg gagccgccgc ccttggcggc cacggtcacc tcgacggtgt cgcccggcac
     7081 gagctcgacg tggaacacgg ccggcgtgtt gtcgcgcgtg ttcttgcgcg cgaacaccgg
     7141 gtcgtccacg acggaggcac gcaggcggtt gtccgggaac tcgtaagcac ggtgcacgcc
     7201 ttcgttcacc atctccgcca ggctgcggcg ggtctcccag cgcacgtcca tgcccacctt
     7261 gaggaacatg ttcacgatgc cggtgtcctg gcagatcggg cggtgccctt cggcgcacat
     7321 gcgcgagttg gtcaggatct gcgcgatcgc gtccttcgcc gccggcgact gctcccgctc
     7381 gtaggcgcgg ccgaggtgct cgaggtagtc gacggggtgg taataggaga tgaactgcag
     7441 cgcgtcggcg acgctctgca cgaagtcgtc ctcgcgaatc acggtcatcg gcggctcctc
     7501 agccgctgag cttgcgcttc agcagctcgt tcagctgtgc gggattggcc ttcccctgcg
     7561 tcgccttcat cacctggccg acgaagtagc cgaagagctt gtccttcccg gaacggtact
     7621 cggcgagctg ctgggggctc ctcgccatca cctcgtcgat cgcccgctcg atcgccgaag
     7681 tgtcggtgat ctgcctcagg ccccgtgcat cgatgatctc gtcggcgccc cggccctcgg
     7741 tccacatcgc ctcgaacact tccttggcga tcttgccgga aatcgtggcg tcgacgatgc
     7801 gcgcgagcag ttgcgcgagc gtggacgcgc cgatacggct ctggccgacc tccaggttct
     7861 cgcggttcag cgcaccggag agatcgccca tgacccagtt cgccgcgagc ttgtgctggc
     7921 cgccgagtcc ctgcacgacg gcctcgtaat agtcggcgag ttcgcggctc gccgtgagca
     7981 cgccggcgtc gtaggcggac agcccgtggt cgcgcacgaa gcgcgccgcc ttctcgtccg
     8041 gcagttccgg caaggccgcg cgcaccgatt cgatcagcgc ctcgtcgatc gcgagcggca
     8101 ggaggtcggg gtccgggaag tagcggtagt cgttcgcctc ctccttggag cgcatcgagc
     8161 gcgtctcgcc gcgatccggg tcgtacagcc gcgtctcctg caccacggtg ccgccaccct
     8221 cgagcaggtc gacctggcgc tcgacctcgt agttgatggc gcgctcgacg aagcggaacg
     8281 agttcaggtt cttgatctcg gcgcgcgtgc cgaacttctc ctgccccctc gggcgcacgg
     8341 agacgttggc atcgcagcgg aaggaccctt cctgcatgtt gccgtcgcag atgccgagat
     8401 agcgcaccag cgtgtggatc ttcttcatgt acgccacggc ttccttcgcg gagcgcatgt
     8461 cgggctccga gacgatttcg agcagcggcg tacccgcgcg attcaggtcg atgccggaca
     8521 acccgtggaa atcctcgtgc agggacttcc ccgcgtcttc ctcgaggtgc gcgcgagtga
     8581 tcccgacgcg cttcaccgtg ccgtcctcga gcacgatctc gagcgtgccg cgctgcacga
     8641 ccggcagctc gtactggctg atctggtagc ccttcggcag gtccgggtag aagtagttct
     8701 tgcgcgcgaa
//
LOCUS       DAOMFD010000083         8641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2532_length_8641_cov_32.392499, whole genome shotgun sequence.
ACCESSION   DAOMFD010000083 DAOMFD010000000
VERSION     DAOMFD010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8641
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2532_length_8641_cov_32.392499"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="PL152_06335"
     CDS             <1..569
                     /locus_tag="PL152_06335"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR48934.1"
                     /translation="DQQRALLRLVTPLTKLMTGKQGVAVVSEVIASFGGAGYVEDTGL
                     PLLLRDTHVLPIWEGTTNVLSLDALLRSDLNAGLAALMGRASSCIRSLTETRLVAAGR
                     SALAALERAALWLESGQDREVLQSGARRLAMTIARALQLALLCEHAQWLIDHEGDRRG
                     YAAALRYARLPVDLMHEVDPELDRILLS"
     gene            complement(637..1476)
                     /locus_tag="PL152_06340"
     CDS             complement(637..1476)
                     /locus_tag="PL152_06340"
                     /inference="COORDINATES: protein motif:HMM:NF021472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HQR48935.1"
                     /translation="MSENEPEAPDATATLPFLAPCRRLALAAPLGWVRRGWRDMWAAP
                     RQSLTYGFLVVCASLALALVALRFGGYWELLSLVTGFILIAPVLAVGTYAISLQLERG
                     ERPSLRRCLREERRALGNLMVFALMLMVVFLVWARAASGIHIFMPLAESPDWSDYLAF
                     FGVGSTVGAVFSGLVFACAAFSLPMLVDRRTDSVTAVVTSVNAVLRNKPAMVVWAACI
                     VVAVAPGFALLFIRHESLGALAWIMAALGVTLPLIGHATWHAYRETIDAAAWPPNDES
                     AGE"
     gene            complement(1568..1903)
                     /locus_tag="PL152_06345"
     CDS             complement(1568..1903)
                     /locus_tag="PL152_06345"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HQR48936.1"
                     /translation="MSTLREMKCTPCPPGTPALAPDAARALLVQLGAGWRISDDGKAL
                     RKAYRFANFHDTMGFVNAVAWIANGEDHHPDLEVGYDYCRMTFATHSIGGLSQNDFIC
                     AAKVDALGA"
     gene            complement(1900..3129)
                     /gene="ispG"
                     /locus_tag="PL152_06350"
                     /gene_synonym="gcpE"
     CDS             complement(1900..3129)
                     /gene="ispG"
                     /locus_tag="PL152_06350"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF001540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0044237 - cellular metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HQR48937.1"
                     /translation="MSPVRRRRSVPVHVGRVTIGGDHPIVVQSMTNTDTADVEATALQ
                     VRALAAAGSELVRVTVNTEEAAAAVPAIAARVRELGAEVPLIGDFHFNGHKLLREHPA
                     CAETLAKYRINPGNVGRGTKRDPQFAEMIEVACRHAKPVRIGVNWGSLDQDLLTRLMD
                     ENSRRADPLDASDVMREAIVVSALDSAARAEDLGLPHDRIVISCKVSGVQDLIGVYRN
                     LAARCDYPLHLGLTEAGMGSKGIVASTAAMSVLLQEGIGDTIRVSLTPDPGGDRTREV
                     VVAQEILQTMGLRSFTPMVVACPGCGRTTSTYFQELAQKIQSHLRSRMPEWRRRYVGV
                     EEMTVAVMGCVVNGPGESKHANIGISLPGTGERPVAPVYVDGEKGPTLKGDRIAEEFQ
                     ALVEQYVERTYGGTTRP"
     gene            complement(3126..4907)
                     /locus_tag="PL152_06355"
     CDS             complement(3126..4907)
                     /locus_tag="PL152_06355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HQR48938.1"
                     /translation="MRSIARTLLPMLVIFAATAAPAARAAENANAKLDALFQSEWERG
                     LRENPLAATYAGDHRYDDRWPDVSADALARSHAGDLEALKKLGTIKPASLTAANRLNQ
                     DLFRRMVQGSVDAYDWGAQYLPINQRGGVQSVSETAGLINFSTAKDYENWIARLNGLG
                     TYVDQNIALLREGARRGIVQPRVIMQRIPDQIAKQVVTDPEQSPFYAPFKEMPDSIPA
                     ADQERLRAAGRKAITDTVVPAYQRLQDYFTKDYLPACKDVVGVWALPGGDAWYQNRIA
                     WFTTTKLTADEVHEIGLEEVARIRGEMQKIIQQVGFQGSFAEFLQYLRTDPKFRYTDP
                     QQLLQAYMVMAKRIDPLLPQYFGKLPRMPYGVRPIPAESAPDTTAAYYQGPGLDGKRA
                     GYYYVNLYKPEDRPKYEIPVLTMHESVPGHHLQIALGMEQGDVPMFRRDFEATAFVEG
                     WALYSESLGQEMGFYDDPYDKFGQLTYEMWRAVRLVVDTGIHSKHWTRDQAIAFFKEN
                     AAKTELDIVNEVDRYIAWPGQALAYKIGELKIKELRARATQVLGPAFDLREFHDVVLG
                     NGAIPLDVLEGNVQRWIDSKQAPERHP"
     regulatory      5031..5182
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            5334..6431
                     /gene="gcvT"
                     /locus_tag="PL152_06360"
     CDS             5334..6431
                     /gene="gcvT"
                     /locus_tag="PL152_06360"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HQR48939.1"
                     /translation="MGLRTPLYETHVAAGARMVDFGGWDMPVNYGSQIEEHHAVRRDA
                     GMFDVSHMCVVDLRGARVRDYLRRLLANDVAKLAQSGKALYSCMLREHGGVIDDLIVY
                     YLDDRWYRVVVNAGTRDKDLEWMRAHAGAYDVSIEPRLDLAMIAVQGPNARQKAAPLL
                     DPQLAARAMDLAAFFGTPGPGGGWFVARTGYTGEDGWEVMLPAAAAAGFWEKLRGAGV
                     APCGLGARDTLRLEAGMNLYGNDMDETVSPLESGLAWTIAWEPQDRDFIGRAALTEQK
                     ARGVGRKQVGLVLADRAVLRSHQKVIVAAVGEGEVTSGTFSPTLERSIGLARVPSQTA
                     DRCEVEIRGRLLPATVVRPPFVRNGRPVIAL"
     gene            6455..6850
                     /gene="gcvH"
                     /locus_tag="PL152_06365"
     CDS             6455..6850
                     /gene="gcvH"
                     /locus_tag="PL152_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HQR48940.1"
                     /translation="MSNVPADLRYMKSHEWARLEADGTITIGISDHAQQALGDLVFAE
                     VPELGRRVAAGEACAVVESVKAASDVYSPVSGEVVASNADLGGQPELINQDPYGAGWL
                     MRIRPDDKAQFAALLDAAGYEAALAAETH"
     gene            6866..8242
                     /gene="gcvPA"
                     /locus_tag="PL152_06370"
     CDS             6866..8242
                     /gene="gcvPA"
                     /locus_tag="PL152_06370"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF001696.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HQR48941.1"
                     /translation="MPFIPHTAEDVRAMLAAIGAPGIDALFDEIPAELRCKGLAGIPP
                     AGSEMQIGRLMSERAARDGRPLNFIGAGAYEHHVPAAVWAIVTRGEFYSAYTPYQAEA
                     SQGTLQLLYEYQSMMTSLTGMDASNASMYDGATALAEACLMAVRAHRKSKSARILLPA
                     SVNPRYREVVQAVAGHQNLAFELLPYDDQSGQLSLSSLDKYRGQDVTALVIQQPNFFG
                     VLEDVDALTDRAHELGMLVIAVVNPVSLAVLKPPGEWGAKGVDIACGEGQPLGVPLSS
                     GGPYFGFLTTRMEHVRQMPGRIIGRTVDLDGKTGFTLTLQAREQHIRRSKATSNICTN
                     QGLLVTAATIYMSLVGPRGLAQVASTSMQRCAELVAALTRIPGVRLAFDRPRFHEALL
                     LLDRPVGAVLEALAARGIVGGFDATRDYPSLGNALLVCATETRTAEDIEAYARALREI
                     MTGAAAAA"
     gene            8251..>8641
                     /locus_tag="PL152_06375"
     CDS             8251..>8641
                     /locus_tag="PL152_06375"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648486.1"
                     /note="acts in conjunction with GvcH to form
                     H-protein-S-aminomethyldihydrolipoyllysine from glycine;
                     forms a heterodimer with subunit 1 to form the P protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQR48942.1"
                     /translation="MHEKLIFEHSTAGRSAEAQYPLEHVAATVPEALRRRSAPLLPEV
                     SELQAVRHFTRLSQLNFSIDTHFYPLGSCTMKYNPRACNQFAMLPEFLARHPYAPAAT
                     GQGILECLYELQEMLKEVTGMRGVSLTP"
BASE COUNT         1298 a         2953 c         3025 g         1365 t
ORIGIN      
        1 acgaccagca gcgcgcgctg ctcaggctcg tgacgccgct caccaagctc atgacaggca
       61 agcagggcgt ggccgtcgtg agcgaggtga tcgcgagctt cggcggtgcg ggctacgtcg
      121 aggacactgg cctgccgctg ctcctgcgcg acacgcacgt gctgccgatc tgggaaggga
      181 ccacgaacgt gctgtcgctc gacgcgctgc tgcgctcgga cctcaacgcc gggctcgcgg
      241 ccctcatggg gcgcgcttcg tcgtgcattc gcagcctgac cgagacgcgc ctggtcgccg
      301 ccggccgcag cgcgctggcc gcgctcgagc gcgcggccct gtggctggaa tccgggcagg
      361 accgcgaagt gctgcagtcg ggcgcgcgac gcctggccat gaccatcgcc cgcgcgctgc
      421 agctggccct gctctgcgag cacgcccagt ggctgatcga ccatgagggc gaccgtcgtg
      481 gctacgcggc cgcactacgg tacgcacggt tgccggtcga cctcatgcac gaggtcgacc
      541 cggagctgga tcgaatcctg ttgtcctgag cgcgcggata cgccagcgcg cggccggagg
      601 aagagcagct cgtgccggag ttgcctccgg gccgggtcat tctcccgccg actcatcgtt
      661 cggcggccag gcggccgcgt cgatcgtctc gcgatacgcg tgccaggttg cgtgaccgat
      721 gagcggcagg gtcacgccca gcgcggccat gatccatgcc agcgcgccga ggctctcgtg
      781 ccggatgaag agcagcgcga accccggcgc caccgcgacg acgatgcacg cagcccacac
      841 caccatggcg ggcttgttgc gcagcaccgc gttgacgctc gtcacgactg ccgtgacgct
      901 gtcggtgcgg cggtccacca gcatcggcag cgagaacgcg gcgcaagcga agacgagtcc
      961 cgaaaagacg gcaccgacgg tgctgccgac gccgaagaaa gcgaggtagt cgctccagtc
     1021 gggcgactcg gcgagcggca tgaagatgtg gatgccggag gcagcgcgcg cccagaccag
     1081 gaacaccacc atcagcatga gcgcgaacac catgaggttg ccgagggccc ggcgttcctc
     1141 ccgcaggcag cgccggagcg acggccgctc cccgcgctcg agctgcaggc tgatcgcgta
     1201 ggtgccgacc gcgaggaccg gggcgatgag gatgaacccc gtgacgagcg agagcagttc
     1261 ccagtagccg ccgaatcgca aggcgaccag cgccagcgcc aggctcgcgc acacgacgag
     1321 gaatccgtag gtcaggctct ggcgcggtgc ggcccacatg tcgcgccaac cgcgcctcac
     1381 ccagccgagc ggagcggcca gggccagcct ccggcacggt gcgagaaacg gcagggtcgc
     1441 cgttgcatcg ggcgcttcgg gctcgttctc gctcatgctt cccccttgtc aggcggcgtc
     1501 tgcgctgcgc cgatggttgc gcaagcgtca ccccgccacg tgacccgacc gcacggtccg
     1561 ccgctaacta cgcccccagt gcgtcgacct tggccgcgca gatgaaatcg ttctgcgaca
     1621 gcccgccgat ggaatgcgtg gcgaaggtca tgcggcagta gtcgtacccg acctcgaggt
     1681 cgggatggtg gtcctcgccg ttggcaatcc aggccacggc gttcacgaag cccatggtgt
     1741 cgtggaagtt ggcgaagcgg tacgccttgc ggagtgcctt gccgtcgtcc gagatgcgcc
     1801 agccggcgcc gagttgtacc agcagcgccc gtgcggcgtc cggggccagc gcgggcgtcc
     1861 cgggcgggca gggggtgcac ttcatttcac gcagcgtgct catgggcgtg tcgtccctcc
     1921 gtatgtgcgc tcgacgtatt gttcgacgag cgcctggaat tcctcggcaa tgcgatcgcc
     1981 cttgagggtc ggtcccttct cgccgtccac gtagacgggc gcgaccggac gctcgccagt
     2041 gcccggcagg ctgatgccga tgttcgcgtg cttgctctcg ccggggccgt tgacgacgca
     2101 gcccatcacg gccacggtca tctcctcgac gcccacgtat cggcgtcgcc actcgggcat
     2161 tcggctgcgc aggtgcgact ggatcttctg cgcgagttcc tggaaatacg tggacgtcgt
     2221 gcgcccgcaa cccgggcagg cgactaccat cggcgtgaac gagcgcaggc ccatcgtctg
     2281 caggatctcc tgggcgacga ccacctcgcg ggtccggtcg ccgccgggat ccggagtgag
     2341 cgagacgcgt atcgtgtcgc cgatgccctc ctgcagcagc accgacatcg cggccgtcga
     2401 ggcgacgatg cccttcgagc ccatgccggc ctcggtgagg ccgaggtgca gcgggtagtc
     2461 gcagcgggca gcgaggttgc ggtagacgcc gatcagatcc tgcacgccgc tcaccttgca
     2521 ggagatgacg atgcggtcgt gcggcaggcc caggtcctcg gcgcgcgccg ccgagtcgag
     2581 cgcggagacc acgatggcct cgcgcatgac gtcgctcgcg tcgagcggat cggcgcgccg
     2641 cgagttctcg tccatgagcc gcgtcaacag gtcctggtcg agactgcccc agttcacgcc
     2701 gatgcgcacg ggctttgcgt gccggcacgc gacctcgatc atctcggcga actgcggatc
     2761 gcgcttggtg ccgcggccga cgttgcccgg attgatgcgg tacttggcga gcgtctcggc
     2821 gcaggccggg tgctcacgca gcagcttgtg tccgttgaag tggaaatcgc cgatcagcgg
     2881 cacctccgcg ccgagctcgc gtacgcgcgc cgcgatggcg gggaccgccg cagcggcttc
     2941 ctcggtgttg accgtgactc ggaccagctc ggagcctgcg gccgccagtg cccgtacctg
     3001 cagcgccgtc gcctcgacgt cggcggtgtc ggtgttggtc atggactgca cgacgatcgg
     3061 gtgatcgccg ccgatcgtca cgcgaccgac gtgcaccggg acgctgcggc ggcgccgtac
     3121 cgggctcatg ggtggcgctc aggtgcctgc ttcgaatcga tccagcgttg cacgttgccc
     3181 tcgagcacgt cgagcgggat cgcgccgttg ccgagcacga cgtcgtggaa ctcgcgcagg
     3241 tcgaacgccg ggcccagcac ctgcgtcgcg cgcgcgcgga gttccttgat cttgagctcg
     3301 ccgatcttgt aggcgagcgc ctggccgggc cacgcgatgt agcgatccac ctcgttgacg
     3361 atgtcgagct cggtcttggc cgcgttctcc ttgaagaagg cgatggcctg gtcgcgagtc
     3421 cagtgcttgc tgtggatccc ggtgtccacg accagccgga ccgcgcgcca catctcgtag
     3481 gtgagctggc cgaacttgtc gtaagggtcg tcgtagaagc ccatctcctg gccgaggctc
     3541 tcgctgtaga gcgcccagcc ctcgacgaac gcggtggcct cgaagtcgcg gcggaacatc
     3601 ggcacgtcgc cctgctccat gccgagtgcg atctgcaggt gatggcccgg cacggattcg
     3661 tgcatggtca gcacgggaat ctcgtacttc ggccggtcct ccggcttgta gaggttgacg
     3721 tagtagtagc ccgcgcgctt gccgtcgagg ccggggccct ggtagtacgc ggccgtcgtg
     3781 tccggcgccg actccgccgg gatcggccgc accccgtagg gcatgcgcgg cagcttgccg
     3841 aagtactggg gcaaaagcgg gtcgatgcgc ttggccatga ccatgtaggc ctgcagcagc
     3901 tgctgcgggt cggtgtagcg gaacttcggg tcggtgcgca ggtactgcag gaactcggcg
     3961 aagctgccct ggaagccgac ctgctggatg atcttctgca tctcgccgcg gatgcgcgcg
     4021 acttcctcga gcccgatctc gtggacttcg tcggcggtga gcttcgtggt cgtgaaccac
     4081 gcgatgcggt tctggtacca ggcgtcgccg ccgggcagcg cccagacgcc gaccacgtcc
     4141 ttgcacgcgg gcaggtagtc cttcgtgaag tagtcctgca gcctctggta ggccgggacc
     4201 acggtgtcgg tgatggcctt gcgtccggcg gcgcgcagtc gttcctggtc cgcggccggg
     4261 atggagtccg gcatctcctt gaacggcgcg tagaacgggc tctgctcggg gtccgtcacg
     4321 acctgcttgg cgatctggtc cggaatgcgc tgcatgatga cccgcggctg cacgattccg
     4381 cggcgcgcgc cttcccgcag cagcgcgatg ttctggtcga cgtacgtgcc gagcccgttc
     4441 aggcgcgcga tccagttctc gtagtccttc gccgtcgaga agttgatcag gcccgcggtc
     4501 tcgctcacgg actgcacgcc cccgcgctgg ttgatcggca ggtactgcgc gccccagtcg
     4561 taggcgtcga cggatccctg gaccatgcgg cggaagaggt cctggttcag gcggttggcc
     4621 gccgtgagcg acgcgggctt gatcgtgccg agcttcttca acgcctcgag gtcgccggcg
     4681 tgggacctgg cgagggcgtc ggccgacacg tccggccagc ggtcgtcgta gcggtggtcg
     4741 ccggcatagg tcgccgccag cgggttttcg cgcaggccgc gctcccactc ggattggaac
     4801 agcgcgtcga gtttcgcgtt ggcattctcc gctgccctgg cggccggcgc ggcggttgcg
     4861 gcgaagatca cgagcatggg tagcagggtg cgggcgatgc tgcgcatggg acgggcgctc
     4921 cgggctggtc agggtcgggg atgttccgtt cccggggccg cgttgacaag tctcgccccc
     4981 ggttcgctgg ctgcggcagc cgcgctcggg taacatcccg ttacccatcg caagacccgt
     5041 ccggagagag aggaaacagg ggacattcac caattttgcg gtcggcggcc tgcccgagcg
     5101 aaattggtga atgtccccgg tttgttccgc cgaaggcgca acccgcccgg aaacgctcag
     5161 gcaaacgaac ggcggtgtct gatgggctct ggagagcggc cccgcgaggg gcccaccgaa
     5221 ggggtgcggc gccggaccac cggcacccga tctctcaggt cacaggacag aggggcggcg
     5281 ggtcttgacg ggcccgccgc cttttttcat ttctggatgc cgggaagaac aacatgggac
     5341 tgcgcactcc gctctacgaa acacacgtcg ccgccggcgc gcggatggtc gacttcggcg
     5401 gctgggacat gccggtcaac tacggctccc agatcgagga gcaccacgcc gtgcgccgcg
     5461 acgcgggcat gttcgacgtg tcgcacatgt gcgtcgtgga cctgcgcggg gcgcgcgtgc
     5521 gggactacct gcgccgcctg ctcgccaacg acgtcgcgaa gctggcgcag tcgggcaagg
     5581 cgctctactc gtgcatgctg cgcgagcacg gcggcgtgat cgacgacctg atcgtgtact
     5641 acctcgacga ccgctggtac cgggtggtcg tcaatgccgg cacgcgcgac aaggacctcg
     5701 aatggatgcg tgcacacgcg ggcgcgtacg acgtgtccat cgagccgcgc ctcgacctcg
     5761 ccatgatcgc cgtgcaggga ccgaacgcgc ggcagaaggc cgcgccgctg ctcgacccgc
     5821 aactcgctgc gcgcgcgatg gacctcgcgg cgttcttcgg taccccgggc ccgggcggcg
     5881 gctggttcgt cgcgcgcacc gggtacaccg gcgaggacgg ctgggaagtg atgttgccgg
     5941 cggcggccgc cgcaggtttc tgggagaaac tgcgcggcgc gggtgtcgca ccctgcggcc
     6001 tgggcgcgcg cgacacgctg cggctcgagg ccggcatgaa cctctacggc aacgacatgg
     6061 acgagacggt ctcgccgctc gaaagcggcc tggcctggac catcgcctgg gagccgcagg
     6121 atcgcgactt catcgggcgc gcggcgctca ccgagcagaa ggcgcgcggc gtcggacgca
     6181 agcaggtcgg cctcgtgctc gccgaccggg ccgtgctgcg ttcgcaccag aaggtgatcg
     6241 tggcggcggt gggcgagggc gaggtcacta gcggcacgtt ctcccccacg ctcgagcgct
     6301 cgatcgggct cgcacgcgtc ccgtcccaga cggccgaccg ctgcgaggtc gagattcgcg
     6361 gccgcctgct gcctgccacc gtcgtgcgcc cgcccttcgt acgcaacggg cggcccgtca
     6421 tcgcactctg aattgcattt cactggagca agccatgagc aacgtccctg ccgacctgcg
     6481 ttacatgaag tcgcacgagt gggcccgcct cgaggcggac ggcaccatca ccatcggcat
     6541 ctcggaccac gctcagcagg cgctgggcga cctcgtcttc gccgaggtcc cggaactcgg
     6601 ccgccgggtc gcggccggcg aggcctgcgc ggtggtcgag tcggtcaagg cggcatccga
     6661 cgtgtacagc ccggtgagcg gcgaggtcgt cgcgtcgaat gcggatctcg gcgggcagcc
     6721 ggaactcatc aaccaggacc cgtacggcgc gggctggctc atgcgcatcc ggcccgacga
     6781 caaggcccag ttcgcggccc tgctcgacgc cgcgggctac gaggccgcgc tcgccgcgga
     6841 aacccactga cggacgggtg agaccatgcc cttcatcccg cacacggcgg aagacgtccg
     6901 cgccatgctg gccgcgatcg gcgcgcccgg catcgatgcg ctgttcgacg agatacccgc
     6961 ggagctgcgc tgcaagggac tcgccggcat cccgccggcc gggtccgaga tgcagatcgg
     7021 ccgcctcatg tccgagcgcg ccgcccgcga cggccggccg ctcaacttca tcggcgcggg
     7081 cgcctacgag caccacgtgc cggcagcggt gtgggccatc gtgacgcgcg gcgagttcta
     7141 cagcgcctac acgccgtacc aggccgaggc cagccagggc accctgcaac tgctctacga
     7201 ataccagtcg atgatgacga gcctgaccgg catggatgcg tcgaacgctt ccatgtacga
     7261 cggcgcgacg gcgctggccg aggcctgcct gatggccgtg cgcgcgcacc gcaagtccaa
     7321 gtcggcgcgg atcctgctgc ctgcgtcggt gaaccctcgc taccgcgaag tggtgcaggc
     7381 cgtggccggc caccagaacc tcgccttcga gctgctgcct tacgacgacc agagcggcca
     7441 gttgagcctg tcgagcctcg acaagtaccg tggccaggat gtcacggcgc tggtgatcca
     7501 gcagccgaac ttcttcggtg tgctcgagga cgtcgacgcg ctgaccgatc gcgcgcacga
     7561 gctcgggatg ctcgtcatcg ccgtcgtcaa cccggtgtcg ctggcggtgc tgaagccgcc
     7621 tggcgagtgg ggcgccaagg gcgtcgacat cgcctgcggc gagggtcagc cgctcggcgt
     7681 gccgctgtct tccggggggc cgtattttgg tttcctgacg acgcgcatgg agcacgtacg
     7741 ccagatgccc gggcgcatca tcggccggac cgtcgacctg gatggcaaga ccggcttcac
     7801 gctcaccctg caggcgcgcg agcagcacat ccgccgcagc aaggccacgt cgaacatctg
     7861 caccaaccag ggattgctgg tgacggcagc cacgatctac atgtcgctcg tcgggccgcg
     7921 cggactcgcg caggtggcat cgacctcgat gcaacgctgc gccgagctcg tcgcggcact
     7981 gaccaggatc ccgggcgtgc ggctcgcgtt cgaccggccg cgctttcacg aggcgctgct
     8041 gctgctcgat cgtcctgtcg gcgcagtcct ggaggcgttg gcagcccgcg ggatcgtggg
     8101 aggcttcgat gcgacccgtg attacccgtc gctcggcaat gcgctcctcg tctgcgcgac
     8161 cgagacgcgc accgccgagg acatcgaggc gtacgcgcgg gcgctgcgcg aaatcatgac
     8221 cggggcggcc gcggccgcct gagaaccgcc atgcacgaaa aactcatttt tgagcactca
     8281 acggccggcc gcagcgccga ggcccagtac ccgctcgagc acgtcgcggc caccgtgccg
     8341 gaagcgctgc gccgccgctc ggcgccgttg ctgccggagg tctccgagct gcaggcggtg
     8401 cgccacttca cgcggctgtc gcagctcaac ttttccatcg acacgcactt ctacccgctc
     8461 gggtcgtgca cgatgaaata caacccgcgg gcgtgcaacc agttcgccat gctgccggag
     8521 ttcctcgcgc ggcacccgta cgcgccggct gcgacgggac aaggcatcct cgagtgcctc
     8581 tatgagctgc aggagatgct gaaggaagtc accggcatgc gcggcgtgtc gctcacgccc
     8641 a
//
LOCUS       DAOMFD010000084         8524 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2602_length_8524_cov_31.565238, whole genome shotgun sequence.
ACCESSION   DAOMFD010000084 DAOMFD010000000
VERSION     DAOMFD010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8524)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8524
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2602_length_8524_cov_31.565238"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2254)
                     /locus_tag="PL152_06380"
     CDS             complement(<1..2254)
                     /locus_tag="PL152_06380"
                     /inference="COORDINATES: protein motif:HMM:TIGR01785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HQR48943.1"
                     /translation="MIHVNCRNSLNLLALAVSMVLSVAARADEPVAPTQQAVASEELE
                     NVTVYARRLVPATRVAATVTVITDQQIQATLAGDVKQMVRYEPGLSVGNDPFRFGLDT
                     FTIRGMTGNRVAVDVDGIPSAPGFAIGSYSNSGRAFVDLALVQRVEILRGPASSLYGS
                     DAIGGVVAMTTFRPDALLGTGAGPALRTAAGYDSSDDGWNAVALAADRFGATDVIVGY
                     VHRQGHEADTAASVTPNPSDYGSDSMLLTAQFDSMPGGPLTVTAEGGRLHQQTDVDAW
                     EELAGSRFVNTVVLKGDDTGERFRTSVAQQLSAATAAYDTANWVLYWQGTDTRQDTYE
                     QRKAVPPPRPGRPGTPPLAINRSFRFEEQVVGAEFTAQKDLERGSVAHDVVYGLEVSG
                     TRLEELRNGTQTDLTTGTTTKTILGETYPLRDFPDSDVVEAGAFAQDEIRFGGGQWSV
                     IPALRIDYYDLSPKPDHVYRADNPRTPVVGLTDTSVSPKLGLTRRMGEHTTAYFQYSN
                     GFRAPPPEDVNIGLDLPLLNIRAIPNPDLKPEKSNGYEVGARFDDGALRFAVAGYWND
                     YDNFIESKVNLGADPVTHVIIFQSQNVERARIYGTEVSAAIRAGAWAPALDGWTARLA
                     AAWSKGEDLDRKEPLNSVDPGSALVSIAYDAPSGAWRGELVTTAVAAKREVDRSRADL
                     YGTNSYVTLDLLGQADLGHGLTLDAGVFNLTNQAYIQWADVRGRPVGDPLIPYYTNPG
                     RNVSVTLRWRY"
     gene            2338..3714
                     /locus_tag="PL152_06385"
     CDS             2338..3714
                     /locus_tag="PL152_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alginate export family protein"
                     /protein_id="HQR48944.1"
                     /translation="MTQVKGIQTLAVAVAVILAAAAAQAADAPTAASAAAPAPAANSL
                     ESALMGGKVHFDFRYRFEDVDQQSFLRDAYASTLRTRLNYTTGEWHGLTAMLEAANVT
                     TLGQYDLYNSTTNGALDRPVVADPRYTEINQAWLQYKYGSFTGIGGRQIINLDNQRFV
                     GNVAWRQNDQTFDAVTLKLGAIPKTQLFYSWVGNVNRIYGPDNGHPIVAPTPANGYAS
                     SGFPGNLESSTSLVNARIDFGAIGALTLFDYFMDFDPNSPYPASKTNNLSNDSYGFYY
                     TGAYKFNDTFKANWVGSWAHQTDYGQSTLNYDADYYMLEGNLGVKNFGFKLGYEVLGG
                     NNPAKVAPGTPGFQTPLATLHTFQGWADMFLTTPTPGIEDFYVGGSATFGALTAQVIY
                     HDFQTEATASYGSYGNEWDASVGYKFGTRYEALVKYATYNADSDVPATLTQSLDTTKT
                     WVQLVATF"
     gene            3799..5070
                     /locus_tag="PL152_06390"
     CDS             3799..5070
                     /locus_tag="PL152_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR48945.1"
                     /translation="MKLETIAVHGGYSPDPTTKAAAVPIYQTTSYAFDDTQHGADLFD
                     LKVAGNIYTRIMNPTTDVLEKRVAALEGGVGALGLASGQAAITYSIQTIVEAGRNIIS
                     SSALYGGTYNLFAHTLPQYGIDVKFGDHRDPASFERLIDANTRAIFCESVGNPAGNVT
                     DFEALSLVAHRHGIPLIVDNTVPTPYLCRPFEHGADIVVHSLTKYLGGHGTSIGGAIV
                     DSGRFPWAQHADKFPRLNTPDVSYHGVIYTEAFGPAAYIGRARVVPLRNMGAAISPFN
                     SFLILQGIETLALRMDRICRNAVAVARFLQVHPKVRWVNYAGLPDHPSHALAQKYMDG
                     RASGILSFGIEGGRAAGARFQDALKLFVRLVNIGDAKSLACHPASTTHRQLSPAELER
                     SGVSEDMVRLSIGIEHQDDLIEDLSQALAAA"
     gene            5142..5783
                     /locus_tag="PL152_06395"
     CDS             5142..5783
                     /locus_tag="PL152_06395"
                     /inference="COORDINATES: protein motif:HMM:NF014997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HQR48946.1"
                     /translation="MTVRAYTRNEEIVHCATHALGAIAILSLIPWLVLAAHSAGDAWR
                     LVSGVVFAVSALLLLTSSIVYHAASSPVAKARLRKLDHSAIYVLIAGTYTPFALGVLR
                     GGWGWSLFGIVWGLALAGVLAKTVFDFRSHVASTLLYLGMGWIGVIAARPLMHVLTGH
                     EQAWILAGGLAYTLGVPFYLWKQRAYTHAVWHVFVLAGIACHSVAVLSVMSRT"
     gene            5874..7676
                     /locus_tag="PL152_06400"
     CDS             5874..7676
                     /locus_tag="PL152_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48947.1"
                     /translation="MHVQTSNHASRRSVLPLLTMFALAACGGGGGGGGGSSGEGGSGG
                     GSGGGSGSGTTSYKILATNDLGMHCVDADFSVFSILPPYNVVNAQVVRTDASGKPTLV
                     DDSSVVLRYSAVADANGSINSHSSDKTNFWRYVASTYGADLPAGQGLKGLYMPAEASP
                     DHPEQTSFSWKSTSGLFKAEGIPIMPIDDAGNVNRYPLMRLTATDKGSGAALATLDVV
                     LPVSEETTCSDCHATGAVAARSSTIAWSSASNLEVQSRENILLLHDSRMGTSLMAAKP
                     VLCASCHYSAALDLAGTGPAGTQVGKPTMSSAMHAYHANKMLDPSGVPYADNWVAQGG
                     TPPSPARQSCYLCHPGRSTQCLRGAMTNAVTCQNCHGGMAAVGGATPLKAGGSFDGAN
                     DGKGRRPWMDLPRCQSCHTGDATNHLTLPDASLMATDGIRAMLAFDSADAAASPRKAT
                     GSRFAENTNTLYRYSKGHGGVACEGCHNSTHAIWPNPTAAHNDNVAANQLQGHSGTIA
                     DCATCHATGTLALGLNGPHGMHVVADSRWRQENGHGGIAKQNRQACAACHGADYRGTA
                     LSRTAAARDWGSRTVAAGTAVGCYDCHNGPGGGD"
     gene            7780..>8524
                     /locus_tag="PL152_06405"
     CDS             7780..>8524
                     /locus_tag="PL152_06405"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQR48948.1"
                     /translation="MDNTPSAFEFARELSPRRNTTTASDIGTLVEEQLGHFAIDPRSE
                     YGRRLGALAASLYSANAAAHELWQLSVRELAALDQRDRVARFNAKRFLCFQLAKILDT
                     LQNPLRKSYQSLLNEPAQSAAKGPYALFDNVTALFSATPVIARTATYVYACTEWVEDA
                     FKGREPLLEIYSRLLNPTSISLANHIVDLEAGPLAGEYLAWNFNSGMAAIDSTLANLV
                     GYQDIVLASRNVYGGTYQLLHDWYGKQSNL"
BASE COUNT         1415 a         2962 c         2808 g         1339 t
ORIGIN      
        1 agtaccgcca gcgcagggtc accgagacgt tgcgcccggg attggtgtag tacgggatca
       61 acgggtcgcc gaccggccgg ccgcgcacgt ccgcccactg gatgtaggcc tggttcgtca
      121 ggttgaagac gccggcgtcg agcgtgaggc cgtggccgag gtccgcctgc cccagcaggt
      181 ccagcgtcac gtaggagttc gtgccgtaca ggtcggcgcg gctgcgatcc acctcgcgct
      241 tggcggccac cgcagtcgtc acgagttcgc cgcgccacgc accggacggc gcgtcgtagg
      301 cgatcgacac gagcgcgctg cccggatcca ccgagttgag cggctctttg cggtcgaggt
      361 cctcgccctt cgaccaggcc gcagccagtc gcgccgtcca cccgtcgagc gccggcgccc
      421 acgcacctgc gcggatggcg gccgacacct cggtgccgta gatgcgcgcg cgctcgacgt
      481 tctgcgactg gaagatgatg acatgcgtga ccgggtcggc cccgagattg accttggact
      541 cgatgaaatt gtcgtagtcg ttccagtagc cggcgaccgc gaatcgcaat gcgccgtcgt
      601 cgaaacgcgc gcccacttcg tagccattgc tcttctccgg cttgaggtcc gggttcggaa
      661 tggcgcggat gttgagcagc ggcaggtcga gaccgatgtt gacgtcctcg ggcggcggcg
      721 cccggaagcc gttcgagtac tggaaatatg ccgtggtgtg ctcgcccatg cgtcgagtca
      781 atccgagctt cggcgagacc gacgtgtccg tgagtcccac gaccggtgtg cgtggattgt
      841 ccgcgcgata gacgtggtcc ggcttggggg acaggtcgta gtagtcgatg cgcaacgccg
      901 ggatgacgga ccactgcccg ccgccaaagc ggatctcgtc ctgcgcgaat gcgcccgcct
      961 cgaccacgtc ggaatcgggg aagtcgcgca gcgggtaggt ctcgccgagg atcgtcttcg
     1021 tcgtggtgcc ggtggtcagg tccgtctgtg tgccgttgcg cagttcctcg agccgtgtcc
     1081 cggagacctc gagcccgtag acgacgtcgt gcgcgacgct gccgcgctcg aggtccttct
     1141 gcgccgtgaa ttccgcgccg acgacctgct cctcgaagcg gaatgagcgg ttgatcgcca
     1201 ggggcggcgt tccgggacgg ccggggcgcg gtggcgggac ggccttgcgc tgctcgtagg
     1261 tgtcctggcg cgtgtcggtg ccctgccagt acagcaccca gttcgcggtg tcgtacgcgg
     1321 ccgtcgcggc ggacagctgt tgcgcgacac tcgtgcgaaa gcgttcgccc gtgtcgtcgc
     1381 ccttcagcac gaccgtgttc acgaagcggc tgcccgccag ttcttcccac gcgtcgacgt
     1441 cggtctgctg gtgcaggcga ccgccttcgg ccgtcaccgt gagcggaccg cccggcatcg
     1501 aatcgaactg cgcggtcagc agcatcgagt cggagccgta gtcgctggga ttcggcgtga
     1561 cgctcgcggc cgtgtccgcc tcgtggccct ggcgatgcac gtagcccacg atcacgtccg
     1621 tggcgccgaa tcgatcggcc gcgagcgcga ccgcattcca tccgtcgtcg ctgctgtcgt
     1681 agccggccgc ggtgcgcagc gccggcccgg ccccggtgcc cagcagcgcg tcgggcctga
     1741 acgtcgtcat cgcgacgacg ccgccgatcg cgtcgctgcc gtagagcgag gacgccggcc
     1801 cgcgcaggat ctcgacgcgc tgcacgagcg cgaggtcgac gaatgcgcgt ccggaattcg
     1861 agtagctgcc gatcgcgaac cccggggcgg acgggatgcc gtccacgtcc acggccaccc
     1921 gattgccggt catgccgcgg atcgtgaacg tgtccagtcc gaagcggaac gggtcgttgc
     1981 cgacgctcaa gcccggctcg tagcgaacca tctgcttgac gtcacccgcc agcgtcgcct
     2041 ggatctgctg gtcggtgatg accgtgacgg tcgccgcgac gcgcgtggca ggtaccaggc
     2101 gccgggcata cacggtcacg ttttcgagtt cctcggatgc caccgcttgc tgcgtcggcg
     2161 ccacgggctc gtcggcgcgc gccgcgaccg acaacaccat cgatacggca agtgccagca
     2221 gattcagtga gttgcggcaa ttgacgtgaa tcaagggaaa tcgtcccgtg ttggagcagt
     2281 ctgttgcacg aagttcctac gtgcacacag cttgaggcat cgcgcccggg agtgaccatg
     2341 acccaggtca aaggcatcca gaccctcgcc gtcgccgtgg cggtaattct tgcagcggcg
     2401 gccgcgcagg ccgcggacgc gccgaccgcg gcatccgccg cggcgcccgc cccagctgcg
     2461 aacagcctcg agtcggcgct gatgggcggg aaggtccatt tcgacttccg gtaccgtttc
     2521 gaggacgtgg accagcagag cttcctgcgc gatgcctacg cctcgacact acgcacgcgc
     2581 ctgaattaca ccaccggaga gtggcacggc ctgaccgcga tgctcgaagc cgcgaacgtg
     2641 accaccctcg gccagtacga cctctacaac agcacgacga acggcgcgct ggaccgcccg
     2701 gtcgtcgcag atcctcgtta taccgagatc aaccaggcct ggctgcagta caagtacggc
     2761 agcttcaccg gcatcggcgg acgccagatc atcaacctcg acaaccagcg cttcgtcggc
     2821 aacgtcgcct ggcggcagaa cgaccagacg ttcgacgcgg tgacgctgaa gctgggcgcg
     2881 atcccgaaga cgcagctctt ctacagctgg gtcggcaacg tgaatcgcat ctacggaccc
     2941 gacaacggcc acccgatcgt ggcgccgacc ccggccaatg gctacgcgtc gtccggcttc
     3001 ccgggcaacc tcgaatcgag taccagcctg gtcaacgccc ggatcgactt cggcgcgatc
     3061 ggggcactga cactgttcga ctacttcatg gacttcgacc cgaattcgcc ctacccggcc
     3121 tccaagacga acaacctctc caacgactcg tacggcttct attacacggg cgcctacaag
     3181 ttcaacgaca cgttcaaggc gaactgggtc gggtcctggg cgcaccagac cgactatggc
     3241 cagagcacac tcaactacga cgccgactac tacatgctcg aaggcaacct cggcgtgaag
     3301 aacttcggct tcaagctggg ctacgaggtc ctcggcggaa acaatcccgc gaaggtcgcc
     3361 ccgggcacgc ccggattcca gacgccgctt gcgacgctgc acactttcca gggctgggcc
     3421 gacatgttcc tcacgacgcc cacgcccggg atcgaggact tctacgtcgg cgggtcggcc
     3481 acgttcggcg cgctgacggc gcaggtcatc taccacgact tccagaccga ggctaccgca
     3541 tcctacggca gctacggcaa cgagtgggac gcgtccgtcg gctacaagtt cggcacgcga
     3601 tacgaggcgc tcgtcaagta cgcgacctac aacgcggaca gcgacgtgcc ggccacgttg
     3661 acgcagtcgc tggacacgac caagacgtgg gtgcaactgg tcgcgacgtt ctgagcccat
     3721 agcccgcggt tctcagctga ggacaggcgc cccggcctgc gggagaatgg cggcgttcca
     3781 ttctcccgca ggtcagtcat gaagctcgaa accatcgccg tccacggcgg ctactccccg
     3841 gacccgacca ccaaggccgc cgccgtcccg atctaccaga ccacgtccta cgcgttcgac
     3901 gacacgcagc acggcgcgga cctgttcgac ctcaaggtcg cgggcaacat ctacacgcgc
     3961 atcatgaacc ccacgaccga cgtgctcgag aagcgcgtcg cggcgctcga gggcggggtc
     4021 ggcgcgctcg ggctggcctc ggggcaggcg gcgatcacgt attcgatcca gacgatcgtc
     4081 gaggccggcc gcaacatcat cagctcctcg gcgctctacg gcggcaccta caacctgttc
     4141 gcccacacgt tgccgcagta cggcatcgac gtgaagttcg gcgaccatcg cgatcccgcc
     4201 tcgttcgaga ggctcatcga cgcgaatacg cgcgcaatct tctgcgagtc ggtgggcaac
     4261 ccggcgggca acgtcacgga cttcgaggcg ttgtcgctgg tggcgcaccg gcacggcatc
     4321 ccgctgatcg tcgacaacac cgtcccgacc ccgtacctgt gccgaccctt cgagcacggc
     4381 gcggacatcg tcgtgcactc gctcaccaag tacctcggcg ggcacggcac cagcatcggt
     4441 ggcgcgatcg tcgactcggg acgcttcccc tgggcgcagc acgccgacaa gttcccgcga
     4501 ctcaacacgc cggacgtctc ctaccacggc gtgatctaca ccgaggcgtt cgggccggcc
     4561 gcctacatcg gccgcgcccg ggtcgtgccg ctcaggaaca tgggcgccgc gatctccccg
     4621 ttcaacagct tcctcatcct gcagggcatc gagacactcg cgctgcgcat ggaccggatc
     4681 tgccgcaatg cggtcgcggt cgcgcgcttc ctgcaggtgc acccgaaggt gcgctgggtc
     4741 aactacgcgg gcctgccgga ccaccccagc cacgcgctcg cccagaagta catggacggt
     4801 cgcgcttccg gcatcctgag cttcgggatc gaaggcggac gtgcggcggg tgcccgattc
     4861 caggacgcgc tcaagctgtt cgtgcggctc gtgaacatcg gcgacgccaa gtcgctcgcg
     4921 tgccacccgg ccagcacgac gcaccggcag ctcagcccag ccgagctcga gaggtccggc
     4981 gtgagcgagg acatggtgcg gctctcgatc ggcatcgagc accaggacga cctgatcgag
     5041 gacctgtcgc aggcgctggc tgcggcatga tcgcgcgcac gtagcgcggc ggtgggcgcg
     5101 aggtggcgcc gcaccgttgc gtccggagag aggagcgtca cgtgaccgta cgggcctaca
     5161 cgcgcaatga agagatcgtc cactgcgcca cgcacgcgct cggtgccatc gcgatcctgt
     5221 cgctgatccc gtggctggtg ctcgcggcgc actcggccgg tgatgcatgg cggctcgtgt
     5281 cgggcgtcgt gttcgccgtc agtgcgctgc tgctgctgac ctcgtcgatc gtgtaccacg
     5341 ccgcatcgag cccggtcgcc aaggctcgcc tgcgcaagct cgatcactcg gccatctacg
     5401 tgctcatcgc gggcacgtac acgccgttcg ccctgggcgt gctgcgcggc ggctggggct
     5461 ggtcgttgtt cggcatcgtg tggggcctgg cgctcgcggg cgtgctcgcg aagaccgtgt
     5521 tcgacttccg ctcgcacgtc gcgtcgacgc tgctctacct cggcatggga tggatcggtg
     5581 tgatcgcggc gcggccgctg atgcacgtgc tcacgggaca tgagcaggcg tggatcctcg
     5641 ccggagggct cgcctacacg ctcggtgtcc cgttctacct gtggaagcaa cgggcctata
     5701 cgcacgcggt gtggcacgtg ttcgtgctgg caggcattgc gtgccacagc gtggccgtgc
     5761 tgagcgtgat gtctcgaacc tgatcaagaa tcgttcgcga ctcgagtcgc gattgatttt
     5821 ccccggcgca tgcgtccaac aggccgagtc ggccatttga agtggggaaa tgcatgcacg
     5881 tccagacctc gaaccacgcg tcacgtcgca gcgtgttgcc gctgctcacc atgtttgcgc
     5941 tcgctgcatg cggcggtggc ggtggaggtg gcggcggttc gtcgggtgaa ggcggctccg
     6001 gcggcggatc gggtggcggc tcgggaagcg gcaccacgtc gtacaagatt ctcgcgacca
     6061 acgacctcgg catgcattgc gtggacgcgg acttctccgt gttctccatc ctgccgccgt
     6121 acaacgtcgt gaacgcacag gtggtgcgca ccgacgcgag cggcaagccc acgctcgtcg
     6181 acgacagcag tgtcgtgctg cgctactcgg ccgtcgccga cgccaatggc tcgatcaaca
     6241 gccacagcag cgacaagacg aacttctggc gctacgtcgc gagcacctac ggtgcggacc
     6301 tcccggccgg ccagggcctg aagggcctgt acatgcccgc cgaggcgagc ccggaccacc
     6361 cggagcagac gtcgttctcc tggaagtcca cgagcggtct cttcaaggcg gaaggcatcc
     6421 cgatcatgcc gatcgacgac gccgggaacg tgaaccgcta tccgctgatg cgcctgaccg
     6481 ccaccgacaa gggcagcgga gcggcgctcg cgaccctcga cgtggtgctg cccgtctccg
     6541 aggagacgac gtgctcggac tgccacgcga cgggcgccgt cgcggcgagg agcagcacca
     6601 tcgcgtggtc gagcgcgagc aacctcgagg tccagagccg ggagaacatc ctcctgctcc
     6661 acgacagtcg catggggacc agcctgatgg ctgcgaagcc cgtgctgtgc gcgagctgcc
     6721 attactctgc cgcgcttgac ctcgccggca ccgggccggc cggcacgcag gtcggcaagc
     6781 cgaccatgtc ttcggccatg cacgcgtatc acgccaacaa gatgctggat ccgtcgggcg
     6841 tgccctacgc cgacaactgg gtcgcgcagg gtggcacgcc gccctcgccc gcgcggcagt
     6901 cgtgctacct ctgccacccc ggtcgcagca cgcaatgcct gcgcggcgcg atgacgaatg
     6961 ccgtgacgtg ccagaactgc cacggcggga tggcggccgt cgggggagcg acgcccctca
     7021 aggctggtgg cagcttcgac ggcgccaacg acggcaaagg tcgccggccg tggatggacc
     7081 tgccgcgctg ccagtcctgc cacaccggcg acgcgaccaa tcacctcacg ctcccagacg
     7141 cgtcgctcat ggcgaccgac ggaatccgcg cgatgcttgc attcgattcc gccgacgcgg
     7201 cggcctcgcc gcgcaaggcg acgggttcgc gattcgcgga gaacacgaac acgctctacc
     7261 gctacagcaa ggggcacggc ggcgtggctt gcgagggctg ccacaacagc acgcacgcga
     7321 tctggccaaa cccgaccgcg gcccacaacg acaacgtggc ggccaaccag ctgcagggac
     7381 attcgggcac gatcgccgac tgcgcgacct gccacgccac gggcacgctc gcgctcgggt
     7441 tgaacgggcc tcacggcatg cacgtcgtcg ccgactcgcg ctggcgccag gaaaacggac
     7501 acggcggcat cgccaagcag aatcgccagg cctgcgccgc gtgtcacggc gcagactacc
     7561 gcgggacggc gttgtcgcgc acggcggctg ctcgtgattg ggggtcacgc accgtcgcgg
     7621 cgggaaccgc cgtaggctgc tacgactgcc acaacggacc gggcggcggc gactgatcaa
     7681 ggcaccgacg gtcggcgggc cccagggccc gccggcccgg ctccccaggg cgaccccgaa
     7741 gcggtatcgg cccgccgacg cgggcggtac gattcgcgga tggacaacac cccttccgcg
     7801 ttcgagttcg cacgcgaact cagcccccgg cgcaatacga cgaccgcctc cgacatcggc
     7861 acgctcgtcg aagagcagct cggacacttc gccatcgacc cgaggtccga gtacggccgc
     7921 cggctcggcg cgcttgccgc cagtctctac tcggcaaatg cggccgcgca cgagctgtgg
     7981 caactgtccg tgcgcgagct cgcggcgctc gaccagcgcg accgcgtggc gcgcttcaac
     8041 gccaagcgct tcctctgttt ccagctcgcg aagatcctcg acacgctgca gaacccgctg
     8101 cgcaagtcgt atcagtcgct gctgaacgag ccggcgcagt ccgcagcgaa ggggccgtac
     8161 gcgctgttcg acaacgtgac cgcgctgttc agcgccacgc cggtcatcgc gcgcacggcc
     8221 acttacgtct atgcctgcac ggagtgggtc gaggacgcgt tcaagggtcg tgagccgctg
     8281 ctggagatct actcgcggct gctcaacccg acgtcgatca gcctggcgaa ccacatcgtc
     8341 gacctcgagg ccggtccgct cgcgggcgag tacctggcgt ggaatttcaa ttcgggcatg
     8401 gccgcgatcg actcgacgct ggcgaacctg gtgggctacc aggacatcgt gcttgcgagc
     8461 cgcaacgtct acggcggcac ctaccagctg ctgcacgact ggtacggcaa gcagagcaac
     8521 ctcg
//
LOCUS       DAOMFD010000085         8488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2628_length_8488_cov_35.301316, whole genome shotgun sequence.
ACCESSION   DAOMFD010000085 DAOMFD010000000
VERSION     DAOMFD010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2628_length_8488_cov_35.301316"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(64..846)
                     /locus_tag="PL152_06410"
     CDS             complement(64..846)
                     /locus_tag="PL152_06410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR48949.1"
                     /translation="MSEKVADPALACRDVEVSVPGRVLLRAFDAVLRPGHMIAVLGRN
                     GAGKSSLLHVLAGLRPPASGAVEWQGRDLQHWPRRDLARHVGFLAQASEDPFPGTVLE
                     SALVGRHPHIGFWQWESDLDRDVARRSLAATDLAGLEDRDVGTLSGGERRRLAIAAVL
                     AQQPRVYLLDEPIQQLDPLHQLEALRLFRARADAGDCVVMSLHDPGIAAFYADESLLL
                     FGDGRWRHGPTSQVLGEASIGELYGIAVRELRWDGGRTFVPA"
     gene            complement(843..1622)
                     /locus_tag="PL152_06415"
     CDS             complement(843..1622)
                     /locus_tag="PL152_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48950.1"
                     /translation="MAERTFLGTMRLALLVFLLVFVALGAWLDRRRSHAWDRTLRVTV
                     YPVSVGAAPVGCAAAVTAADFDDATEFLRAQAATYGVALDVPVNFSVSHAAKEPPPEL
                     SAAPGPLAIAYWSLRTRWWARRVAFHDPLPKPDVQVFAVYHADDGQQAAPDSTGLSKG
                     LIAVAHLFCGPDAVSGNALVVTHELLHTLGASDKYDRATGQPVAPDGLGSPAQSPLYP
                     QRTGEIMAGRIAVSPANAVIPGSLRDMVVGPATAREIGWSR"
     gene            complement(1626..2270)
                     /gene="pdxH"
                     /locus_tag="PL152_06420"
     CDS             complement(1626..2270)
                     /gene="pdxH"
                     /locus_tag="PL152_06420"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF004231.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine-phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HQR48951.1"
                     /translation="MLHKATLPAKLPSDPFPLFDEWFAHARQHSTQRNPDAMVVATAT
                     PDGQPSARVVLLKRLVAAPGYVVFYTNYDSRKGHEIAANPRVAAVVYWDALGRQVRLE
                     GRVVKSPAGESDEYFASRALDSRIGAWASLQSQPLESRTQLMKRVAAQAARFGTSVPR
                     PPHWGGYRLWPEAVELWHEGAFRVHDRARWTRRLDPSGEADFVAGPWAATRLYP"
     gene            2383..3495
                     /locus_tag="PL152_06425"
     CDS             2383..3495
                     /locus_tag="PL152_06425"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HQR48952.1"
                     /translation="MKSLEDLRARLDVLDRELLELVAERQALSTEIAAVKRATGQSTR
                     DFRREREVLLKARRDAEALGVSPSLAESILHTLIRGSLTTQEQARVAAQGRGTGRAAL
                     VIGGGGKMGHWTADFLASQGFRVTIADPSGAVPGYEWVSDWSESRLDQDVIVVATPLK
                     AANDVLLALATRQPRGVVFDIGSLKTPLRAGLAALRAAGVCVTSVHPMFGPDTDLLSG
                     RHVIFVDLGDARALAEARELFASTMADLVVMGLDEHDRLIAFVLGLSHALNIAFFTAL
                     AESGEAAPRLARMSSTTFDAQLDVATRVAAENPELYYEIQSLNAYGEVSLRALRTAVD
                     RLCQAVHEHDQRGFVDMMRRGHEYLMVRQAAREQVA"
     gene            3492..4508
                     /locus_tag="PL152_06430"
     CDS             3492..4508
                     /locus_tag="PL152_06430"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HQR48953.1"
                     /translation="MTLPDALAKILDWERTLLGAGSFAAWLQAAAGPPDARTDDAVSL
                     ILADPTDELRHLTAGADRVQHAVLPVSYVDSLAGRAPQFDALHGPWAGAYRVVDHGLL
                     FPAALGLTHLLMLPLLRGGQLAGVYSLGGRSGPPALSSADAGLLEHAATVIATSLERS
                     FDRARLLRGGHADPLTGWNSARYLQVRLREEIARCQRHGGSVACLVADVDRLQQVNDE
                     HGQPAGDLALTEIADRIESQVRACDAAGRIGSDVFGVVLPATDAARAVPLAERVLAAV
                     SRAPVDLGGGQQRTLSVSIGIAALAPAPAADRKALSEEVIANAIAAMHRAKQRGGGGY
                     EIAP"
     gene            complement(4519..5502)
                     /locus_tag="PL152_06435"
     CDS             complement(4519..5502)
                     /locus_tag="PL152_06435"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HQR48954.1"
                     /translation="MNDRIRLPAVLILLGLLATVLVALSLAIGSSRLAPADIVDALVS
                     GTPSLARDVVLDLRLPRAMSALAVGGLLALAGAMLQVLLRNPLADPYVLGISGGASVF
                     ALLAIMVGLPGAGVDGAAALGAFVTCLVVFALAQGRGGWTPTRLLLTGIVVAAGTGAV
                     VSLLLATSDDAGLRGMVFWLLGDLSRARGWPWLLALLGVAAAVGMLLGRHLNVLARGM
                     TQAASVGLDVRRFQMLLFVLSSVLTGVAVSAAGSIGFVGLVTPHLVRLMLGSDNRVVL
                     PVSALLGGILLVAADLASRVLLAPRQLPVGALTALVGVPIFLVLMGRQRPA"
     gene            5632..6093
                     /locus_tag="PL152_06440"
                     /pseudo
     CDS             5632..6093
                     /locus_tag="PL152_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016144233.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
     gene            6231..7010
                     /locus_tag="PL152_06445"
     CDS             6231..7010
                     /locus_tag="PL152_06445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48955.1"
                     /translation="MADAAPEGGWMRRGPAFLPRAAWVAIAVFAAAGSFWLGYRQGQR
                     FAGYDAAAGADERRRLLEQVSKLESANTDLVAKVAELEMSRRLDREAYGQVERTLGDL
                     QSRLARQGDDLAFYRSIVSPADGVQGLRIQRFAVRPGTGPREYVIEVTLIQAMRQDNT
                     ISGLAQVVVQGMQSSRPAHYTFGELSGQPQAQLPFSFRYFQTIQREVTLPVDFQPFEV
                     EIRAKSGRSGTPVVQSFPWKVSGSGGDAAAAPTDPPRGTVR"
     gene            7074..7427
                     /gene="erpA"
                     /locus_tag="PL152_06450"
     CDS             7074..7427
                     /gene="erpA"
                     /locus_tag="PL152_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HQR48956.1"
                     /translation="MQEPSATPEPVLVFTDSAAVKVSELIREEGNPNLKLRVFVSGGG
                     CSGFQYGFTFDENEEEGDTCVVNQGVKLLIDPMSFQYLVGAEIDYKEDLDGAQFVIRN
                     PNATTTCGCGSSFSA"
     gene            7435..>8488
                     /gene="ppx"
                     /locus_tag="PL152_06455"
     CDS             7435..>8488
                     /gene="ppx"
                     /locus_tag="PL152_06455"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR03706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004309 - exopolyphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006798 - polyphosphate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQR48957.1"
                     /translation="MARPDTTPPDVLAAVDLGSNSFHMVVARYSHGQLTIVDRLRESV
                     RLSAGMDEQGRLSRDSTTRALACLERFGQRLRDVKAKRVRVVGTNTLRKAKRKGAFLD
                     RAREALGHPIEIISGVEEARLIYHGVARTTPSQGGHRLVVDIGGGSTELIVGEGLAAR
                     RLESLYIGCVSMMEEHFADGDITEKRMKRARTAAQLEFEPVMARFRRSEWDAAFGASG
                     TMRAVSDVLAARGAPAGEISREGVDWLVAECLRAGSVGRLKLPALTADRLEILPGGVA
                     IMVEAFDMLGIESLRVADGALREGLLYDLLGRLTDEDARVRSVRAMEARFHVEQAQAD
                     RVETTALGLLRQVREPW"
BASE COUNT         1277 a         2988 c         3085 g         1138 t
ORIGIN      
        1 agctgcagac gttcgacgac tggctcgccg caaacgtgca gcgcatcccg acgggctgag
       61 ccctcacgcc gggacgaagg tccggccgcc gtcccagcgc agctcgcgca ccgcgatccc
      121 gtagagctcg ccgatcgacg cctcgccgag cacctgcgac gtcgggccgt gtcgccagcg
      181 gccgtcgccg aacagcagca gcgactcgtc ggcatagaac gccgcgatgc cggggtcgtg
      241 caggctcatg acgacgcagt cgccggcgtc ggcacgggcg cggaacagac gcagcgcctc
      301 gagctggtgc agcgggtcga gctgctggat gggctcgtcg aggaggtaga cgcgcggctg
      361 ctgcgcgagc acggcggcga tcgcgagtcg ccgacgctct ccgccagaca gcgtgcccac
      421 gtcgcgatcc tcgaggccgg cgaggtccgt tgccgcgagt gaacgccgcg cgacgtcgcg
      481 atccaggtcg ctttcccact gccagaatcc gatgtgcgga tgccggccga cgagcgcgga
      541 ctcgagcacc gtgccgggaa acggatcctc gctggcctgc gccaggaagc cgacgtgccg
      601 cgcgagatcc cggcgtggcc agtgctgcag gtcgcggccc tgccactcga ccgcgccgct
      661 cgcgggcggc ctgaggcccg ccagcacgtg cagcaacgaa ctcttgccgg cgccgttgcg
      721 accgagcacc gcgatcatgt gaccaggccg cagcaccgcg tcgaaggcgc gcaacagcac
      781 gcgcccgggc acgctcacct cgacgtcgcg gcaggcgagg gctgggtccg ccactttctc
      841 actcatcggg accacccgat ttcgcgcgcc gtggcggggc cgacgaccat gtcgcgcagg
      901 ctccccggaa tgaccgcgtt agccgggctc acggcgatgc ggcccgccat gatctcgccc
      961 gtgcgctgcg ggtagagggg cgactgcgcc gggctgccga gaccgtccgg agccacaggc
     1021 tggccggtgg cgcggtcgta cttgtcgctc gcgccgagcg tatgcagcaa ctcatgggtg
     1081 acgaccaacg cgttgccgct gaccgcatcc ggtccgcaga acaggtgcgc gactgcgatc
     1141 agtcctttcg atagtccggt cgagtccggc gcggcctgct ggccgtcatc ggcgtggtac
     1201 acggcgaaga cctgcacgtc cggcttcggc agcgggtcgt ggaaggccac gcgccgtgcc
     1261 caccagcgcg tccgcaggct ccagtaggca atggcaagcg gacccggcgc ggcggaaagc
     1321 tccggcggcg gctccttcgc ggcatgcgag acgctgaaat tcaccgggac gtcgagcgcg
     1381 acgccgtagg tcgcggcctg tgcacggagg aattccgtgg cgtcgtcgaa gtctgcggcg
     1441 gtcacggcgg ccgcgcagcc gacgggcgct gcaccgacgg acaccgggta caccgtcacg
     1501 cgcagcgtcc ggtcccacgc gtggctgcga cgacgatcca gccacgctcc cagtgccacg
     1561 aagacgagca ggaagacgag cagcgcgagg cgcatcgtgc cgaggaaagt gcgctcggcc
     1621 acggctcaag ggtagaggcg tgttgcggcc caggggcccg cgacgaaatc ggcctcaccc
     1681 gaagggtcga ggcgccgcgt ccagcgtgcg cggtcgtgca cgcggaaggc gccttcgtgc
     1741 cagagctcga cggcctcggg ccacaggcgg tagccgcccc agtgcggcgg ccgtggaacc
     1801 gaggtcccga agcgcgcggc ctgagccgcc acccgcttca tgagctgcgt gcgcgactcg
     1861 agcggctggc tctgcaggct cgcccacgcg ccgatgcggc tgtcgagcgc acggctcgcg
     1921 aaatactcgt cgctctcccc ggcgggcgac ttgacgaccc gtccctccag gcggacctgc
     1981 cgcccgaggg cgtcccagta caccactgcg gcgactcgcg ggttcgcggc gatctcgtgc
     2041 cccttgcggc tgtcgtagtt ggtgtagaag acgacgtacc ccggcgcggc cacgagccgc
     2101 ttgaggagca cgacgcgagc cgacggctgc ccgtccggcg tggccgttgc gacgaccatg
     2161 gcgtccgggt tgcgctgcgt cgaatgctgc cgcgcatgcg cgaaccactc gtcgaaaagt
     2221 gggaacgggt ccgagggcag cttcgcgggc aaggtcgcct tgtggagcat cggcaggttc
     2281 cgggcgggtc gagcacggat tatgcccgag cgcgcgcgcc gcctgcttgc agccggcatt
     2341 gccgaccggt aaaagaaacc ggataacgct tgcggacgct ccatgaaatc actcgaagac
     2401 ctgcgtgcgc ggctcgacgt cctggaccgc gagctgctcg aactcgtggc cgaacgccag
     2461 gcgctcagca cggaaatcgc ggccgtgaag cgggccacgg gacagtccac gcgggacttc
     2521 cgccgcgagc gcgaggtgct gctcaaggcg cggcgcgacg ccgaggcgct cggggtctcg
     2581 ccgtcgctcg ccgagtccat cctgcacacg ctgattcgcg gctccctcac cacgcaggag
     2641 caggcaaggg tcgccgccca gggccgcggc acgggtcgcg cggcgctggt gatcggcggc
     2701 ggaggcaaga tggggcactg gacggccgac tttctcgcct cgcagggatt ccgcgtgacc
     2761 atcgccgacc cgtccggcgc ggtgccgggc tacgaatggg tctccgactg gtccgagtca
     2821 cgcctcgacc aggatgtcat cgtggtcgcc acgccgctca aggccgccaa cgacgtgctg
     2881 ctcgcgctcg cgacgcgcca gcccagggga gtcgtgttcg acatcggttc gctgaagacg
     2941 ccgctgcgtg ccgggctcgc ggcattgcgc gccgccggcg tgtgcgtgac gtcggtgcac
     3001 ccgatgttcg gtccggacac cgaccttctg tccgggcgcc acgtcatctt cgttgacctc
     3061 ggcgatgcgc gcgcgctggc ggaggcgcgc gagctgttcg catcgacgat ggccgacctc
     3121 gtggtgatgg ggctcgacga gcacgaccgg ctgatcgcgt tcgtgctcgg cctgtcgcac
     3181 gcgctcaaca tcgcgttctt caccgcgctg gccgagagcg gcgaggcggc gccgcgcctg
     3241 gcgcgcatgt cgagcacgac cttcgacgcg cagctcgacg tcgcgacgcg ggtcgccgcc
     3301 gagaacccgg agctctacta cgaaatccag agcctcaatg cctatggcga ggtatcgctc
     3361 cgggctctgc gcaccgctgt cgaccggctc tgccaggcgg ttcacgagca cgaccagcgc
     3421 ggattcgtgg acatgatgcg gcgcgggcac gaatacctca tggtgcggca ggcagcgcgc
     3481 gagcaggtcg catgacgctg ccggacgcgc tcgcgaaaat cctcgactgg gagcgcacgc
     3541 tgctcggcgc cggttcgttt gccgcgtggc tgcaggccgc cgccgggccg cccgatgcgc
     3601 gcaccgacga cgcggtgtcg ttgatcctcg cggacccgac cgacgaattg cggcacctca
     3661 cggccggcgc ggaccgtgtg cagcacgccg tgctaccggt gtcgtacgtc gacagcctcg
     3721 cgggtcgcgc accgcagttc gatgcgctcc acgggccctg ggcgggcgcg taccgggtag
     3781 tggaccacgg gttgctgttc cctgccgcgc tcggcctcac gcacctgctc atgctcccgc
     3841 tgctccgcgg cgggcagctc gccggtgtct acagcctcgg cggacgcagc gggccgcccg
     3901 cgctgtcgtc ggccgacgcg ggcctgctcg agcacgcggc cacggtgatc gcgacgagcc
     3961 tcgagcggtc gttcgaccgc gcgcggctgc tgcgcggcgg ccacgcggat cccctcacgg
     4021 gctggaactc ggctcgctac ctgcaggtac ggctgcggga ggaaatcgcg cgctgccagc
     4081 gccacggcgg cagcgtcgcg tgcctggtcg cggacgtgga tcgcctgcag caggtgaacg
     4141 acgagcacgg ccagccggcc ggcgacctcg cgctcaccga gatcgcggac cggatcgaat
     4201 cgcaggtgcg cgcgtgcgat gcggccggtc gcatcggcag cgacgttttc ggcgtcgtgt
     4261 tgcccgcgac cgacgcggcg cgcgcggtgc cgctcgcgga acgcgtgctc gccgcggtgt
     4321 cccgcgcgcc cgtcgacctg ggcggcggcc agcaacggac gctcagcgtc tcgatcggca
     4381 tcgccgcgct cgcgccggct cccgcggcgg accgcaaggc gctgtcggag gaggtcatcg
     4441 ccaacgcgat tgcggccatg caccgcgcca agcagcgcgg tggcggaggt tacgagatcg
     4501 cgccctgagt ctgcctgctc acgccgggcg ctgccggccc atcagcacca ggaagatcgg
     4561 cacgccgacc agcgcggtga gcgcgccgac cggcagctgc cggggcgcga gcagcaccct
     4621 tgacgcgagg tccgccgcca cgagcaggat gccgccgagc agcgccgaca ccggcagcac
     4681 gacgcgattg tcgctgccga gcatcagccg cacgaggtgg ggcgtcacga ggcccacgaa
     4741 gccgatgctg ccagcggcac tcaccgcgac acccgtcagg accgagctca acacgaacaa
     4801 cagcatctgg aagcggcgca cgtcgagccc cacgctcgcg gcctgcgtca tgccgcgtgc
     4861 cagtacgttg aggtgacgcc cgaggagcat gcccacggcc gccgcgacgc cgagcagagc
     4921 cagcagccaa ggccagcccc tggcgcgcga caggtcgccg agcagccaga acaccatgcc
     4981 gcgcaggcct gcgtcgtcgc tcgtcgcgag cagcaggctc accaccgcgc cggtgcccgc
     5041 ggcaaccacg ataccggtga gcagcaggcg cgtcggcgtc cagccgccgc ggccctgcgc
     5101 gagcgcgaac acgaccaggc aggtcacgaa agcgccgagc gccgcggcgc cgtcgacgcc
     5161 ggcgccaggc agcccgacca tgatcgcgag cagcgcgaag acggaggcgc cgccggaaat
     5221 gccgaggaca tagggatcgg cgagcggatt gcgcagcagg acctgcagca tcgcgccggc
     5281 aagtgccagc agcccgccca ccgccagcgc cgacatcgcg cgcggcagcc ggaggtcgag
     5341 gacgacgtca cgcgccagcg agggcgtgcc cgagaccagc gcatccacga tgtcggcggg
     5401 tgcaaggcgg gaactgccga tcgccagcga gagggcgacg aggaccgtcg cgagcaggcc
     5461 gagcaggatc aatacggcag ggaggcgtat ccggtcgttc atgggggtgc ggcgagcgtc
     5521 cggcaagggt aggtgccacc cgtcgcggcg acaaggctca ccgcggcggc aggcccggcg
     5581 cgcggtgcgc cacgatatga aaagctgaat gaactctgta agttacgctg catggctgac
     5641 ttcatcgacc cgcagggatt tcgcgccaat gtcgggatcg tgctgatccg cgacggcggc
     5701 gaggtcttcc tcggcagtcg cagcgacggc cgcggctggc agttccccca gggcggcgtg
     5761 cggcgcaacg agtcgcccga ggacgcgctg ttccgcgagc tgcacgagga agtcggcctc
     5821 gagccgggcg acgtcgaggt gctggcgggg acccggagct ggctgcgcta ccggctgccc
     5881 cggcagtacg tccgccggcg ctcgcggccg ctgtgcatcg gccagaagca gcgctggttc
     5941 ctgctgcgca tggtcggggg cgacgagcgg ctccggttcg acgtcacgcc gacacccgag
     6001 ttcgatgcgt ggcgctgggt cgactactgg gcgccggtcc gcgaagtgat ctacttcaag
     6061 cgcatggtct acgcacgggc gctgggcgaa ctcgcgttgt cggcgtttcc agccggcatt
     6121 ccgccgtacc cggactggtg gtcggagtcg gtgctgcggc cgccgatgcc gcaacgaccg
     6181 cgccacgccc agcgtgcgcg ggagagcgag ccggaggagg ccgggaagta atggcggacg
     6241 ccgcgcccga gggtgggtgg atgcggcgcg gcccggcctt cctgccgcgc gcggcctggg
     6301 tcgcgatcgc ggtattcgcg gcagcgggct cgttctggct cggttaccgg cagggtcagc
     6361 gcttcgcggg atacgacgcg gcggccggcg cggacgagcg acgccggttg ctcgagcagg
     6421 tgtcgaaact cgagtctgcg aacaccgatc tcgtggccaa ggtggctgag ctcgagatgt
     6481 cgcggcgact cgaccgcgag gcctacgggc aggtcgagcg cacgctcggc gatctccagt
     6541 cccgcctcgc gcggcagggc gacgatctcg cgttctaccg cagcatcgtc tcgccggcgg
     6601 acggcgtgca gggcctgcgc atccagcgct tcgcggtgcg ccccggcacc gggccgcgcg
     6661 agtacgtcat cgaggtgacc ctgatccagg cgatgcggca ggacaacacg atctcgggcc
     6721 tcgcgcaggt cgtggtgcag ggcatgcaga gcagccgtcc ggcgcactac acgttcggcg
     6781 agctgtcggg ccagccgcag gcgcagctgc cgttctcgtt ccggtacttc cagacgatcc
     6841 agcgggaggt gaccctgccc gtggatttcc agcctttcga ggtggagata cgcgcgaagt
     6901 cgggccggtc gggcaccccg gtcgtgcaat cgttcccctg gaaggtctcc ggcagcgggg
     6961 gcgatgcggc tgccgcgccc actgacccgc cccggggcac cgtgcggtga gcggcggtcc
     7021 caggtgctaa aatccacggc ccgtatcctt tgagtcgcgc cgcaatgacc cagatgcaag
     7081 agcccagcgc caccccggag ccggtcctgg ttttcacgga ctcggccgcc gtcaaggtga
     7141 gcgagctcat ccgcgaggaa ggcaatccca acctcaagct gcgcgtcttc gtgtcgggcg
     7201 gcggctgctc gggcttccag tatggcttca cgttcgacga gaacgaggaa gaaggcgaca
     7261 cctgcgtcgt gaaccagggc gtcaagctcc tgatcgaccc gatgagcttc cagtacctcg
     7321 tcggtgccga gatcgactac aaggaggatc tcgacggcgc gcagttcgtg atccgcaatc
     7381 ccaacgcgac gaccacctgc ggctgcgggt cgtccttctc ggcctgacgc gggattggcc
     7441 cgccccgaca ccaccccgcc cgacgtcctc gcggctgtcg acctcgggtc caacagcttc
     7501 cacatggtgg tcgcgcgcta ctcgcacggc cagctcacca tcgtcgaccg cctgcgcgag
     7561 tccgtgcgcc tctccgccgg catggacgag cagggccgcc tgagccgcga ctcgaccacg
     7621 cgcgcgctcg cgtgcctcga gcgcttcggg cagcggctgc gcgacgtcaa ggccaagcgg
     7681 gtgcgcgtgg tgggcaccaa cacgctgcgc aaggcgaagc gcaagggcgc attcctcgac
     7741 cgcgcgcgcg aggcgctcgg ccacccgatc gagatcatct ccggcgtcga ggaggcgcgg
     7801 ctcatctacc acggcgtggc gcgcacgacg ccgagccagg gcgggcaccg gctggtcgtg
     7861 gacatcggcg gcggcagcac cgagctgatc gtgggcgagg ggctcgcggc gcggcggctc
     7921 gagagcctgt acatcggctg tgtgagcatg atggaggaac acttcgccga cggcgacatc
     7981 accgagaagc gcatgaagcg cgcgcggaca gccgcgcagc tcgagttcga gccggtcatg
     8041 gcgcggttcc gccgctccga gtgggacgcg gcgttcggcg catccggcac gatgcgcgcg
     8101 gtgagcgacg tgctcgccgc caggggcgcg ccggcgggcg agatttcccg cgagggcgtc
     8161 gactggctgg tcgcggagtg cttgcgcgcc ggcagcgtcg gcaggctcaa gctcccggcg
     8221 ctcacggcgg accgcctgga gatcctgccc ggcggtgtcg cgatcatggt cgaggccttc
     8281 gacatgctcg gcatcgagtc gctgcgcgtc gcggacggcg ccctgcgcga gggcctgctg
     8341 tacgacctgc tcgggcgact gaccgacgag gacgcgcgcg tgcgcagcgt ccgcgcgatg
     8401 gaggcccgct tccacgtgga gcaggcgcag gcggaccgcg tcgagacgac cgcgctcggc
     8461 ctgctccggc aggtgcggga accgtggg
//
LOCUS       DAOMFD010000086         8488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2629_length_8488_cov_33.982687, whole genome shotgun sequence.
ACCESSION   DAOMFD010000086 DAOMFD010000000
VERSION     DAOMFD010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2629_length_8488_cov_33.982687"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1967
                     /locus_tag="PL152_06460"
     CDS             <1..1967
                     /locus_tag="PL152_06460"
                     /inference="COORDINATES: protein motif:HMM:NF037276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48958.1"
                     /translation="DIHQVSFNQSHDMDPAVLDDGRVVYSRWDGAPGHDEISLYTMRP
                     DGSDLELLYGANSHATGTDGADIEFLDPRPTGNGHVLVRAQPMQSQDLGGELLDIDVA
                     NYVENTQPTAANSGVLAGPAQVPATTNDVRTVDGPSPGGRFGSAFPLRDGTGRLLVTW
                     SQCRLLEGTRIVPCTADRLAAAGATVAPPLYGLWMYDPSKRTQLPVADPVEGIVYSEV
                     VALQPTPLPPVLLDRVAGVDFDDELANEDVGILNIRSVYDVDGVDTAPGGIATLADPK
                     VTLAAARPARFLKIEKAVSLPDRDVRDFRASAFGVTSAFGMREILGYAPVEPDGSVRI
                     KVPANVALAISVLDSSGRRIGTRHNNWLQVRPGEEVRCNGCHDPASNLSHGRSDVFAT
                     VHAGAPTTGLPYPNTNPAYFADFGETMAEVRTRISCQTDCAALLPSTSVAFVDEWTDP
                     VAAGRVPDASFAYRYPDLSTPAPTSTQCMTVWSAGCRITINYERNIHPLWSLPRVTLA
                     ADGVTVLSDDTCTSCHSNRTAAGALQVPPGQLELTDGPSPDVPDQFHAYRELLATDNE
                     QELVGGVLQDRQVQVGVDPVTQQPQFATVPVAPSLRAGNANASTDFFSLFAAGGSHAG
                     RLTPAELRLISEWVDLGAQYYNDPFAAPVN"
     gene            1972..2715
                     /locus_tag="PL152_06465"
     CDS             1972..2715
                     /locus_tag="PL152_06465"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HQR48959.1"
                     /translation="MRAILITALALLATSAPAVAGGGAAVASVVVQDAYIDLHSGPGR
                     GYPVTQVVERGGSVVLLRQRTDWIKVRTDRDYEGWVNRSQLERTLAPDGSAVRLAGPE
                     PDARTRHRWEVSIGAGDFDGADTITAGASFALTDSLFARADVSQLLGDYSNGWLGTVG
                     IAHSFVPEWRVSPFVGIGGGVVYVDPNATLVQTSHRTNSTAYAGAGLRGYLTDRFLLQ
                     AEYRQYVVFMDTNDNEVISEWTVGFTHYF"
     gene            2685..>3340
                     /locus_tag="PL152_06470"
     CDS             2685..>3340
                     /locus_tag="PL152_06470"
                     /inference="COORDINATES: protein motif:HMM:TIGR04565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel domain-containing
                     protein"
                     /protein_id="HQR48960.1"
                     /translation="MDRRLHPLLLNAAALACLLAGPAPSRAEDTLPAAADTPQVVEPK
                     VHRREVTVPAIDTENFEAGLFVGTISIEDFGTSMLYGGRIAYHFTEDVYAEATAGTAK
                     AGKTSYEDLSGSAQLLTSSERQYTYYDLALGWNALPGEIYLGGHRAMPSAVYLSLGAG
                     STHFAGDNHFTLTPGAGFRLLATDWLAAHLDFRDQMFNSDLLGKDKLTHNLQFNLGMT
                     A"
     assembly_gap    3341..3440
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3441..3689
                     /locus_tag="PL152_06475"
     CDS             <3441..3689
                     /locus_tag="PL152_06475"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR48961.1"
                     /translation="IVGINVEPDSDAAQKWLAQVPVSLTFPILFDRENKVAESFGVDG
                     MPSTVFIDRTGAVRYVHRGYRPGDEAKYADMVRSLVKE"
     gene            3695..3919
                     /locus_tag="PL152_06480"
     CDS             3695..3919
                     /locus_tag="PL152_06480"
                     /inference="COORDINATES: protein motif:HMM:NF025452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4266 domain-containing protein"
                     /protein_id="HQR48962.1"
                     /translation="MVRSLIVSLPLFVATAGCSNVEPWVKPYERQNFADLIMAADRDP
                     VSLAYIEHVYEAREGARGALGGHGGGCGCN"
     gene            3906..5147
                     /locus_tag="PL152_06485"
     CDS             3906..5147
                     /locus_tag="PL152_06485"
                     /inference="COORDINATES: protein motif:HMM:NF023516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3570 domain-containing protein"
                     /protein_id="HQR48963.1"
                     /translation="MAATESDDQRTTGTAAADAASPAATENDRATSRGRSDASTLAML
                     PFALAALAPAAKAATLPDDRADAMYHRYEGGGVTVDGPSLLVRKKLGDNFSVAANYYV
                     DMVSSASIDVVTQASPYKEERRQMSLAVDAIRGKTQYTVAYVNSTENDYQANTASFDL
                     SQTMFGDLTTVSLGFSRAWDEVEKRGDPSFKEPVDRWSYRFGVSQILTSRLMMGLSLE
                     TITDEGYLHNPYRSARYLDPDVAAGFAWEPEVYPNTHNSTAVTLDGDYYVWYRAAVHG
                     MYRYYTDSWGVSGNTFRIGYTHPIGERWILEADYRWYDQTGADFYSDLFPRENSQNFV
                     GRDKELSTFQSQMFTFGVTWRLPPLKWGGLDRTTVNVFYDHALYDYADFRNILKGGPP
                     GEEPLYKMSADVLRVFFSAWF"
     gene            complement(5144..6238)
                     /gene="aguA"
                     /locus_tag="PL152_06490"
     CDS             complement(5144..6238)
                     /gene="aguA"
                     /locus_tag="PL152_06490"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: protein motif:HMM:NF010070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0047632 - agmatine deiminase activity
                     [Evidence IEA];
                     GO_process: GO:0009446 - putrescine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HQR48964.1"
                     /translation="MTRTLSTDPRRDGYRMPGEFERHSGCWMLWPERPDNWRLGGKPA
                     QQAFTAVAVAIAASEPVTVGVSVGQFSNARRQLPPAVRVVELSSNDAWMRDVGPTFVV
                     NGRGGVRGVDWQFNAWGGLDGGLYFPWDKDDAVAGKVLEVEGRDRYRAPFILEGGAIH
                     VDGQGTLITTEECLLNRNRNPQFTRPEIERLLERYLGVEAIVWLGRGVHLDETDGHVD
                     NLCAFVRPAEVVLSWCDDRRDPQYEISRDAYERLIEARDARGRRFKVHKLVQPGPLHM
                     TKREAEGIDFGAGAKPREAGARLAGSYVNFYIGNRRVVVPLLDSKRDGAALRKLRELF
                     PGREVVGVPAREILLGGGNIHCITQQVPAG"
     gene            complement(6302..8377)
                     /locus_tag="PL152_06495"
     CDS             complement(6302..8377)
                     /locus_tag="PL152_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016451564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HQR48965.1"
                     /translation="MCPAVANDRPGACPHCGMALEPALPSLADGEDPEFKDMRRRFVV
                     ALVLTIPLFALAMAPMAGWHPPLAAAWLQFALATPVVLWCGWPLLERGARSLVTRRLN
                     MFTLIGLGVVVAYGYSVWATLAPGTVPHAFHHGSEAALYFESAAMIVTLVLLGQVLEL
                     RARGRTGAALRSLMDLAPKTARRLEADGREVEVALTDVRIGDQLRVRPGERVPVDGEV
                     LEGRGAVDESMVTGESLPVEKAVGAALIGGTLNGQGSLVMRAARIGRDTVLARIVQAV
                     AEAQRSRAPVQALADRVSAWFVPAVVTVAAVSAIVWAIVGPEPRLANALLIAVSTLIV
                     ACPCALGLATPMSMTVAMGRGARAGVLFRDAAALQALEEVDTVVLDKTGTLTAGRPEV
                     VTVIPVEGVPEAELLRFAASLEQASEHPLAGAIVRAAQARSLRLGRTYGFEAKAGAGA
                     TGVVQMRRVAVGNARLMEEAGADVGALEPEADRLRELGQTTVFVAVDRVPLGLIGIAD
                     PLKPDSWEAVQEFKRLGLHVVLLSGDHAASVRAVAQMLGIDEVHAGVAPEGKATVLRE
                     LRAGGRKVAMAGDGINDAPALAEADVGIAMGGGTDIAKQTAGVTLISGDLRGIARALE
                     LSEATMSNVRQNLLFAFGYNALGVPLAAGALYPLTGMLLAPAAAAAAMSLSSVSVITN
                     ALRLGRVRL"
BASE COUNT         1336 a         3103 c         2740 g         1209 t
ORIGIN      
        1 gcgacatcca ccaggtgtcg ttcaaccaga gccacgacat ggatcccgcg gtgctcgacg
       61 acggccgcgt cgtgtacagc cgctgggacg gcgcgcccgg acacgacgag atcagcctct
      121 acacgatgcg ccccgacggc agcgacctcg agttgctcta cggcgccaac agccatgcga
      181 cgggcacgga cggcgcggac atcgagttcc tcgacccgcg cccgacgggc aacggccacg
      241 tgctggtgcg cgcgcagccg atgcagtcgc aggacctcgg tggcgagctg ctcgacatcg
      301 acgtcgccaa ctacgtcgag aacacccagc cgaccgcggc caactcgggc gtcctggccg
      361 gtccggcgca ggtgccggcc acgaccaacg acgtgcgcac cgtggacgga ccgtccccgg
      421 gcgggcgatt cggctccgcg tttccgctgc gcgacggcac cgggcgcctg ctcgtgacct
      481 ggtcgcagtg ccgtctcctc gaaggcacgc gcatcgtgcc gtgcaccgcc gaccgcctcg
      541 cggcggccgg ggcgaccgtt gcgccgccgc tctacggcct gtggatgtac gacccgtcca
      601 agcgcaccca gctcccggtc gccgatccgg tcgaagggat tgtgtactcg gaagtcgtgg
      661 cgctgcagcc gaccccgctg ccgcccgtgc tgctcgaccg ggtcgcaggc gtcgatttcg
      721 acgacgaact cgcgaacgag gacgtcggca tcctcaacat ccgcagcgtc tatgacgtcg
      781 acggcgtgga taccgcgccc ggcggcatcg cgaccctcgc cgatcccaag gtcacgctcg
      841 cagcggcgcg cccggcgcga ttcctcaaga tcgagaaggc ggtgagcctg ccggatcgtg
      901 acgtgcgtga cttccgcgcc agcgcgttcg gcgtcacctc cgcgttcggc atgcgcgaga
      961 tcctgggcta cgctccggtc gagcccgacg ggtcggtgcg catcaaggtt cctgcgaacg
     1021 tggcgctggc catctcggtg ctcgactcga gcggccggcg catcgggaca cggcacaaca
     1081 actggctgca ggtccgaccc ggcgaggaag tgcgctgcaa cggctgtcac gaccccgcca
     1141 gcaacctgtc ccacggccgg tcggacgtgt tcgcgacggt gcacgcgggc gcgccgacga
     1201 cgggcctgcc gtatccgaac acgaacccgg cgtatttcgc cgatttcggc gagaccatgg
     1261 ccgaggtgcg cacgcgtatc agctgccaga ccgattgcgc ggcgctgctg ccgtcgacca
     1321 gcgttgcgtt cgtggacgag tggaccgatc cggttgcggc cggccgcgtg cccgacgctt
     1381 cgttcgccta ccgctatccg gacctgtcga cacccgcacc gaccagcacc caatgcatga
     1441 cggtgtggtc ggcaggctgc cgcatcacca tcaactacga gcgcaacatc cacccgctct
     1501 ggagcctgcc gcgcgtgacg ctcgcggccg acggcgtcac cgtgctgtcc gacgacacgt
     1561 gcacgagctg ccactcgaat cgcacggcgg ccggggcact gcaggtgcca ccgggccagc
     1621 tcgagctcac cgacggcccg tcgcccgacg tgcccgacca gttccacgcg taccgcgagc
     1681 tgctcgcgac ggacaacgag caggaactcg tgggcggcgt gctgcaggac cggcaggtgc
     1741 aggtcggcgt cgatccggtc acccagcagc cgcagttcgc cacggtgccg gtggctcctt
     1801 ccctgcgcgc cggcaacgcc aacgcgtcca cggacttctt ttccttgttc gccgctggcg
     1861 gcagccatgc gggtcgactc acgcccgcgg agctcaggct gatctccgaa tgggtggacc
     1921 tcggcgccca gtactacaac gacccgttcg ccgcgccggt gaactgaagc gatgcgagcg
     1981 atcctcatca ccgccctggc cctgctcgcc acgtccgctc cggccgtggc cggcggcggc
     2041 gccgcggttg ccagcgtagt cgtgcaggac gcctacatcg acctgcacag cgggcccggg
     2101 cgcggctacc cggtgaccca ggtcgtggag cgcggcggga gcgtcgtgct gctcaggcag
     2161 cgcacggact ggatcaaggt gcgcacggac cgcgactacg agggctgggt gaaccgcagc
     2221 cagctcgagc gcacgctcgc ccccgacggg tcggcggtgc gactggcagg cccggagccc
     2281 gacgcgcgga ccaggcatcg ctgggaagtg agcatcggcg cgggcgactt cgacggtgcc
     2341 gacacgatca ccgccggcgc ctcgttcgcg ctcaccgatt cgctcttcgc gcgcgctgac
     2401 gtgtcgcagc tgctcggcga ctactccaac ggctggctcg gcaccgtcgg catcgcgcat
     2461 tccttcgtgc cggaatggcg ggtgtcgccg ttcgtcggga tcggcggcgg cgtcgtgtac
     2521 gtggacccca acgcgacgct cgtccagacg tcgcatcgca ccaactcgac cgcgtatgcc
     2581 ggagcggggc tgcgcggcta cctgaccgac cgcttcctgc tgcaggcgga gtaccgccag
     2641 tacgtggtct tcatggacac caacgacaac gaggtcatca gcgaatggac cgtcggcttc
     2701 acccactact tctgaacgcg gcggcgctcg cgtgcctgct ggccgggccg gcgccgtccc
     2761 gtgcggagga cacgctaccc gccgcggcgg acacgccgca ggtcgtcgag cccaaggtgc
     2821 accggcgcga agtcacggtg cccgcgatcg acacggagaa cttcgaggcg ggcctgttcg
     2881 tcggcacgat cagcatcgag gacttcggca ccagcatgct gtacggcggg cgcatcgcct
     2941 atcacttcac cgaggacgtg tacgctgaag ccacggcggg caccgccaag gccgggaaga
     3001 ccagctacga ggacctgagc gggtcggcgc agctgctcac cagcagcgaa cggcagtaca
     3061 cctactacga cctcgcgctc ggctggaacg cgctgcccgg cgagatctac ctcggcggcc
     3121 accgcgccat gccgagtgcc gtgtacctgt cgctcggggc cggcagcacg cacttcgccg
     3181 gcgacaacca tttcacgctg acaccgggcg ccggcttccg cctgctcgcc acggactggc
     3241 tggcggcgca cctcgacttc cgcgaccaga tgttcaacag cgacctgctg ggcaaggaca
     3301 agctcacgca caacctgcag ttcaacctcg gcatgaccgc nnnnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn atcgtcggca tcaacgtcga gcccgacagc gacgccgcgc
     3481 agaagtggct cgcccaggtg ccggtgtcgc tgaccttccc gatcctgttc gaccgcgaga
     3541 acaaggtggc cgaatcgttc ggggtggacg gcatgccgag cacggtgttc atcgaccgca
     3601 ccggcgccgt gcgctacgtg caccgcggct accgccccgg cgacgaagcc aagtacgccg
     3661 acatggtccg cagcctcgtg aaggagtgat gcgcatggtc cgcagcctca tcgtctcgct
     3721 cccactgttc gtagccaccg ctggatgcag caacgtcgag ccgtgggtga agccatacga
     3781 gcggcagaac ttcgcggacc tgatcatggc ggccgaccgc gacccggtct cgctcgccta
     3841 catcgagcac gtctacgagg cgcgcgaagg cgcgcgcggg gcgctcggcg gtcacggagg
     3901 cggctgtggc tgcaactgag tcagacgacc agcgcacgac cggcaccgcc gccgcggacg
     3961 ccgcgtcgcc tgccgcgacg gagaacgatc gcgcgacgtc gcgcggcagg agcgacgcct
     4021 cgacgctggc catgctgccc tttgcgctgg ccgcgcttgc gccggcggcc aaggctgcga
     4081 cgctgccgga cgaccgcgcc gacgcgatgt accaccgcta cgagggtggc ggcgtcacgg
     4141 tcgacggtcc ctcgctgctg gtgcgcaaga agctgggaga caatttctcg gtcgccgcga
     4201 attactacgt cgacatggtg tcgagcgcgt cgatcgacgt cgtgacccag gcgagcccgt
     4261 acaaggagga gcgccgccag atgagcctgg cggtggacgc catccggggc aagacccagt
     4321 acaccgtcgc ctacgtgaac agcaccgaga acgactacca ggccaacacc gcctccttcg
     4381 acctgagcca gacgatgttc ggtgacctga ccacggtctc gctgggattc agccgcgcgt
     4441 gggacgaggt cgaaaagcgc ggcgacccca gcttcaagga gccggtggat cgctggagct
     4501 accgcttcgg cgtctcgcag atcctgacct cccgcctgat gatgggcctg tcgctcgaga
     4561 ccatcaccga cgagggctac ctgcacaacc cgtatcgctc tgcgcgctac ctcgaccccg
     4621 acgtcgcggc gggcttcgcg tgggagcccg aggtttaccc gaacacgcac aacagcaccg
     4681 ccgttacgct cgacggcgac tactacgtct ggtatcgcgc ggccgtgcac ggcatgtatc
     4741 gctattacac cgactcctgg ggcgtgtcgg gcaacacgtt ccggatcggc tacacgcacc
     4801 cgatcggcga gcgctggatc ctcgaagccg actaccgctg gtacgaccag acgggcgccg
     4861 acttctactc cgacctcttc ccacgcgaga actcgcagaa tttcgtcggc cgcgacaagg
     4921 aactgagcac gttccagagc cagatgttca cgttcggcgt cacctggcgc ctgcctccgc
     4981 tcaagtgggg cggcctcgac cgcaccaccg tcaacgtctt ctacgaccac gcgctctacg
     5041 actacgcgga cttccgcaac atcctgaagg gtggtccgcc cggcgaggaa cccctgtaca
     5101 agatgagcgc ggacgtgctg cgggtgttct tctcggcctg gttctagccg gccggcacct
     5161 gctgcgtgat gcagtggatg ttgccgccgc cgagcaggat ctcgcgcgcc ggcaccccga
     5221 cgacctcccg cccgggaaac agttcgcgga gcttgcggag cgccgcgccg tcgcgcttcg
     5281 aatcgagcag cggcacgacg acccgtcggt tgccgatgta gaaattcacg taggaacccg
     5341 cgagccgcgc gcccgcctcg cgcggcttgg cgcctgcgcc aaagtcgatc ccctctgcct
     5401 cgcgtttcgt catgtgcagc ggacccggct ggaccagctt gtggaccttg aagcgtcgtc
     5461 cgcgcgcatc gcgcgcctcg atcagccgct cgtacgcgtc gcgtgagatc tcgtactggg
     5521 gatcacggcg atcatcacac cacgacagca cgacctccgc tggacgcacg aatgcgcaga
     5581 ggttgtccac gtggccgtcc gtctcgtcga ggtgcacgcc gcgaccgagc cagacgatcg
     5641 cctcgacacc gaggtagcgc tcgagcagcc gctcgatctc gggcctcgtg aactgcgggt
     5701 tgcgattgcg gttgagcagg cactcctccg tcgtgatcaa cgtgccctgc ccgtcgacgt
     5761 ggatcgcgcc gccctcgagg atgaacggcg cgcgataccg gtcgcgcccc tcgacctcga
     5821 gcactttccc ggccacggcg tcgtccttgt cccaggggaa atagagcccg ccgtccaggc
     5881 ctccccaggc attgaactgc cagtccacgc cgcgcacgcc gccgcgcccg ttcacgacga
     5941 acgtggggcc cacgtcgcgc atccaggcgt cgttgctcga cagttcgacc acgcggacgg
     6001 ccggcggcag ctgccggcgc gcgttggaaa actgtccgac agaaacaccc accgtgaccg
     6061 gctcgctggc cgcgatggcg acagcgaccg ccgtgaacgc ctgctgcgcc ggcttgccgc
     6121 ccaggcgcca gttgtccggc cgctccggcc acagcatcca gcagccggag tgccgctcga
     6181 actcgccggg catcctgtag ccgtcgcgtc ggggatcggt cgagagtgtg cgggtcacgt
     6241 tgcgtcctcc aggcaccgcg gacacaccag cccgcggtcc gctccacagg ctcttgaggc
     6301 tctagagccg cacccggccg agtcgcagcg cgttggtgat cacgctgacg gagctcagcg
     6361 acatcgccgc cgcggccgcg gcgggcgcga gcagcatgcc ggtgagcgga tacagcgcgc
     6421 cggcggccag cggcacgccg agcgcattgt agccaaaggc gaacagcagg ttctggcgca
     6481 cgttggacat cgtcgcctcg ctcagctcga gcgcgcgcgc gatgccacgc aggtcgccac
     6541 tgatcagcgt cacccctgca gtctgcttgg caatgtcggt gccgccgccc attgcaatgc
     6601 ccacgtcggc ctcggccagt gccggcgcgt cgttgatacc gtcgcctgcc atggcaacct
     6661 tacgcccgcc cgctcgcagc tcgcgcaaca ccgtcgcctt gccttcaggc gcgaccccgg
     6721 cgtgaacctc gtcgatgccc agcatctgcg cgacagcgcg caccgaggcc gcgtggtcgc
     6781 cggacagcag cacgacgtgc aggcccagtc gcttgaattc ctgcaccgct tcccagctgt
     6841 cgggcttcag cggatcggcg atgccgatga gacccagcgg cacccgatcc acggcgacga
     6901 acaccgtcgt ctggcccagc tcgcgcaagc ggtccgcctc cggttcgagc gccccgacgt
     6961 cggcgcccgc ctcctccatc agtcgcgcat tgccgaccgc gacgcggcgc atctgcacga
     7021 ctcctgtcgc accggcgccc gccttcgctt cgaatccata ggttcgcccg aggcgcaggc
     7081 tcctggcctg cgccgcgcgc acgatggccc cggcgagcgg gtgctcgctg gcctgctcca
     7141 ggctggcggc gaaacgcagc agttccgctt cagggacccc ttccacgggg atgacggtca
     7201 cgacctccgg ccggccggcc gtcagcgtgc cggtcttgtc cagcaccacc gtgtccacct
     7261 cctcgagcgc ctgcagcgcg gccgcgtcgc ggaacagcac gcccgcgcgc gcaccacggc
     7321 ccatcgccac ggtcatggac atcggcgtcg ccagtccgag cgcgcacggg cacgccacga
     7381 tgagcgtact caccgcgatg agcagcgcgt tcgcgaggcg cggctcgggg ccgacgatgg
     7441 cccagacgat tgccgatacc gcagcgacgg tgaccaccgc tggcacgaac caggcggaca
     7501 cccggtccgc cagcgcctgg accggcgccc ggctgcgctg cgcctccgcg accgcctgga
     7561 cgatgcgtgc gagcaccgtg tcccgcccga tgcgcgccgc acgcatgacc agcgatccct
     7621 gcccgttcaa cgtgccgccg atgagcgcgg cgcccaccgc cttctcgacc ggaagtgact
     7681 cgccggtgac catcgactcg tccaccgcgc cgcggccctc gaggacctcg ccatccacgg
     7741 gcacgcgctc acccggccgc acccgcagct ggtcgccgat gcgcacgtcg gtcagcgcca
     7801 cctcgacctc gcgaccgtct gcctccaatc ggcgcgcggt cttcggcgcc aggtccatca
     7861 gggaccgcaa tgccgcgccg gtccggcccc gggcccgcag ttccagcacc tgccccagca
     7921 gtaccagcgt cacgatcatc gccgccgact cgaaatacag cgcggcctcg ctgccatggt
     7981 ggaacgcatg cggtaccgtg ccgggcgcga gtgtggccca tacgctgtac ccgtacgcga
     8041 ccacgacacc caggccgatc aacgtgaaca tgttgagtcg tcgcgtcacg agcgaccgcg
     8101 ccccgcgctc gagcagaggc cacccgcacc acagcacgac cggcgtcgcg agcgcgaatt
     8161 gcagccacgc ggcggcgagc ggtggatgcc atcccgccat tggggccatg gcgagtgcaa
     8221 atagcgggat cgtgagcacc agcgccacga cgaatctccg gcgcatgtcc ttgaactctg
     8281 gatcctcgcc atcggccagt gacggcagcg cgggctcgag cgccatgccg caatgggggc
     8341 aggctccggg acggtcattc gccaccgctg ggcacatcgg acagatgtaa agggccttcc
     8401 cgccatgcgg cgcagttgcc acgcgcgccg gcggcgggac acccgcgacc ggagcgccgt
     8461 ggcagcagtc gtgcgtcgca ggcttggc
//
LOCUS       DAOMFD010000087         8442 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2650_length_8442_cov_31.568380, whole genome shotgun sequence.
ACCESSION   DAOMFD010000087 DAOMFD010000000
VERSION     DAOMFD010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8442)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2650_length_8442_cov_31.568380"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..954
                     /locus_tag="PL152_06500"
     CDS             <1..954
                     /locus_tag="PL152_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48966.1"
                     /translation="QKPIEGVSMAYSFDDAKAPSTRHTQYFEMFGLRAVYHDGWVACT
                     TPPTPLGEKADPTVDIITGYQWELYDTTNDFSEAVNLADKYPDKLKEMQLLFYSEAAK
                     YNVLPLDNQRIPRTDVSIRPSLTRGRTEFTYYGTIARIPEGAAPNLKNKSFRITADVT
                     LPKGDESGVVLTQGGLSAGWALMFQGGRPVFHYNFGNVAHYQVAAASAVPPGKHVVVF
                     DFKYDGGGIGKGGTGTISVDGQQVAQGRIEHTMAFRLSLDETFDVGEDTGTPVSLDYD
                     VPFAFAGTLGKVVVQLGDTKLSAADQQRIQSVEQAAMRAVE"
     gene            984..1538
                     /locus_tag="PL152_06505"
     CDS             984..1538
                     /locus_tag="PL152_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48967.1"
                     /translation="MHCCNSSTDRGYWLLALALVLTVLSSAPDTAKSAPAAASGAQAR
                     GAAGAGDAGDDTRCVDALFQLEVLAGDWKVYKAGPGGERVYLDDRDRPAEIERLTRER
                     DDNCSADPEALASQKQRASDLFQAMSPDCLEAREKLLNLQRPTARSAPSDIEKQQAWV
                     TAHCPDVSRTGVWLADWRYPQQKR"
     gene            complement(1567..1776)
                     /locus_tag="PL152_06510"
     CDS             complement(1567..1776)
                     /locus_tag="PL152_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48968.1"
                     /translation="MKRFDPGQEPSAWGSFDDAEALRRWSFERRTPEQRIAWLKAALE
                     LAYQSGALKPRRPDCADAQSLRTDR"
     gene            complement(1781..2272)
                     /locus_tag="PL152_06515"
     CDS             complement(1781..2272)
                     /locus_tag="PL152_06515"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HQR48969.1"
                     /translation="MPTDFIALFAVLAGARVRFVVVGGLALVLHGLDRLTADVDIVID
                     LSPESTLDVVRALTAAGYKPLAPVDPARLADPEQRREWQATRHMQVFSFWDSTNLRPT
                     VDVMLEPAVPFAELWAAASLVDIGGCRVPIASIGHLIQLKEAAGRPQDQADIDRLKSM
                     LAE"
     gene            2370..3032
                     /locus_tag="PL152_06520"
     CDS             2370..3032
                     /locus_tag="PL152_06520"
                     /inference="COORDINATES: protein motif:HMM:NF037096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HQR48970.1"
                     /translation="MTDYERLKERQRAERHAHPESLALRVHRALSWLHRAEQLGINDD
                     LDGQFIFLWIAFNAAYATDIDEKYRDSEQQTFRAFLEKLTALDQGKRRFELLTWQEFP
                     KSIRVLLDNPYVFADFWKFQSGALGEDEWRRNFDTANHAARKALGSQDTVSVLAIVLA
                     RIYTLRNQLVHGGATWQSSVNREQLRDCTSFMAKLVPLVIEVMMDHPDTLWGQGTYPV
                     VR"
     assembly_gap    3114..3213
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3214..3512
                     /locus_tag="PL152_06525"
     CDS             <3214..3512
                     /locus_tag="PL152_06525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48971.1"
                     /translation="AQYDALTHHLDQDYEDDSEVITQARASVNRLRNMNYPGIERVLA
                     HFAGIPDEGYEKAFFAFRDTDLFRDTLRPRVEIEYPRLRRMADDITARVGNGYR"
     gene            complement(3547..3981)
                     /locus_tag="PL152_06530"
     CDS             complement(3547..3981)
                     /locus_tag="PL152_06530"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HQR48972.1"
                     /translation="MSAFVLDASVTMSWLLGDARPADDAYAAAVLDSLRQPDCTALVP
                     MTWALEIGNVVVRAEARGLVDEAQSEAFLEMLAGVAIQPDDATFARALTDTLHVARRY
                     ALSSYDASYLELALRAGAPIATLDADLSKSARKAGVKRFTPH"
     gene            complement(3978..4235)
                     /locus_tag="PL152_06535"
     CDS             complement(3978..4235)
                     /locus_tag="PL152_06535"
                     /inference="COORDINATES: protein motif:HMM:NF014643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HQR48973.1"
                     /translation="MTVTTEIGAYEAKTRLPELLREVRAGRRFTITNRGEAVADLVPS
                     TAAISADAATAIDRFRAFMREHPLKARRRASVRALIEEGRA"
     gene            complement(4518..5222)
                     /locus_tag="PL152_06540"
     CDS             complement(4518..5222)
                     /locus_tag="PL152_06540"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HQR48974.1"
                     /translation="MGRTSFDRRQLSRSKLDLFLECPRCFYEDLVRHNGRPGGPAFTL
                     NIAVDELFKREFDAHRARQEPHPLFATVGLDAVPLQDSRLPQWRHNFTGVRWLDPDTG
                     WTLYGAVDDLWLGADGRVMVADYKATSKAEHVTSATLHPAYRRQADVYQFLVAQQGLE
                     VSDRAWFVYANGLKTADGFHDTLRFDTRLVPYDGNRSWVAAAFRDAVSLALSGQLPDP
                     GPECAWCKFAANWSGG"
     gene            complement(5227..6012)
                     /locus_tag="PL152_06545"
     CDS             complement(5227..6012)
                     /locus_tag="PL152_06545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48975.1"
                     /translation="MILSRIIEHVKRQHWTAIGIDFVIVVVGVFIANQVTTWKKEAEL
                     SRRKAAALERLHAESEAVVDYITERATMFAKDNALREDALQRLGDDDWAGADTEKVSE
                     AFGSIDLAPAASPPRSVYDELISTGLFAEIGDAKLRDAVADYYAYLLFLQGQIDYVRQ
                     GITAGPGLQDFKGIRLSYDRKEFRGVRTDFDFHALSADKAFVDYAIMRNADQVAQQQW
                     WTLTLFKARAMCAEIARYDGRPCRPKTSGQGNEIFGTTDAPGT"
     gene            6231..6998
                     /locus_tag="PL152_06550"
     CDS             6231..6998
                     /locus_tag="PL152_06550"
                     /inference="COORDINATES: protein motif:HMM:NF018949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrU family protein"
                     /protein_id="HQR48976.1"
                     /translation="MSRILAFLYGVVCYAVFFATFLYLMGFIAGAYVPKGINDGIVTA
                     PIVAIVIDLALVAAFGVQHSVMARPGFKSWLTRFVSPAVERSTFVLAASLVLVLLYWQ
                     WRPLPTVVWSVSDPTAVELLWVLYLAGFGLVLAASFVIDHFDLFGLRQVWLHLLRRQY
                     VHPQFTVTWIYRFVRHPIYLGLLLAFWSAPTMTVGRLVFALGMSVYILIGIRYEERDL
                     VHFIGEDYRRYRERVPMLVPRPGATHEVVKPARVGDA"
     gene            complement(7003..7380)
                     /locus_tag="PL152_06555"
     CDS             complement(7003..7380)
                     /locus_tag="PL152_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008095668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajD family HNH nuclease"
                     /protein_id="HQR48977.1"
                     /translation="MTTRKRVEPDHARLDRIVAEARQARQERERTYRGQALKLFPWIC
                     GRCAREFDHTNVHELTVHHKDHDHDNNPPDGSNWELLCLYCHDNEHQRQLDMRPLTDG
                     SASAPGATHTPFAELASLLKPDD"
     gene            7628..7852
                     /locus_tag="PL152_06560"
     CDS             7628..7852
                     /locus_tag="PL152_06560"
                     /inference="COORDINATES: protein motif:HMM:NF018961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dodecin family protein"
                     /protein_id="HQR48978.1"
                     /translation="MAKKKADHGVYRVTEVIGTSRKSWEDAAASAVEAAAKSLRDLRI
                     AEVSKLDMKIEGGKVVAYRARVLLSFKYEG"
     gene            7973..>8442
                     /locus_tag="PL152_06565"
     CDS             7973..>8442
                     /locus_tag="PL152_06565"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HQR48979.1"
                     /translation="MSAQACPVSDHARRTARRVADLLANADALLITAGSGMSAECVPA
                     DDPELRELQARFEAQGRSFREMAQPYRFDQHADLAWGWYGHRQRWYRRMRPHDGYRIV
                     RSWCQAMSCGSFVATTNIDGQFFEAGFTDWQVLERHGSLLHCQCTQPCCDEVWSA"
BASE COUNT         1352 a         2749 c         2877 g         1364 t
ORIGIN      
        1 cagaagccga tcgagggcgt gagcatggcg tacagcttcg acgacgcgaa ggcgccgtcg
       61 acccgtcaca cgcagtactt cgagatgttc ggcctgcgtg ccgtctacca cgacggctgg
      121 gtcgcgtgca cgacgccccc gactccgctc ggcgagaagg ccgaccccac ggtggacatc
      181 atcaccggct accagtggga gctgtacgac acgacgaacg acttcagcga ggcggtgaac
      241 ctggcggaca agtacccgga caagctgaag gaaatgcagc tgctcttcta ttcggaggcg
      301 gcgaagtaca acgtgctgcc gctcgacaac cagcgcatcc cgcgcaccga cgtctcgatc
      361 cgtcccagcc tgacgcgcgg ccggacggag ttcacctact acgggacgat cgcgcgcatt
      421 ccggaaggag ctgcgcccaa cctcaagaac aagtcgttcc gcatcacggc cgacgtgacg
      481 ctgcccaagg gcgatgagag cggcgtggtc ctcacgcagg gcgggctcag cgccggctgg
      541 gcgctgatgt tccagggcgg ccgtccggtc ttccactaca acttcggcaa tgtcgcgcac
      601 taccaggtcg ccgccgcgag cgcggttccg cccggcaagc acgtcgtcgt gttcgacttc
      661 aaatacgacg gcggtggcat cggcaagggc ggcaccggca cgatcagcgt ggacggccag
      721 caggtcgcgc agggccgcat cgagcacacg atggcgttcc ggctgtcgct cgacgagacg
      781 ttcgacgtgg gcgaggacac cggtacgccg gtgagtctcg actacgacgt gccgttcgca
      841 ttcgccggga ccctcggcaa ggtcgtcgtc cagctcggcg acacgaagct ctccgcggcg
      901 gaccagcagc ggatccagag cgtcgagcag gcggcgatgc gggccgtcga gtaggggacg
      961 aaagagcagg aggggtcaat cgcatgcact gctgcaattc aagcaccgac cgcggctact
     1021 ggctgctggc attggcgctg gtcctgacgg tgctatcgtc tgcgcccgac acggcgaagt
     1081 cggctccagc ggcggcgtcc ggcgctcagg ctcggggcgc tgcgggtgcg ggcgatgccg
     1141 gtgacgacac ccggtgcgtg gatgcgttgt tccagctgga ggtgctggcg ggcgactgga
     1201 aggtctacaa ggcggggccc ggcggcgagc gcgtctacct ggatgatcgc gatcgtccgg
     1261 cggagatcga gcggttgaca cgtgagcgag atgacaactg cagtgccgac ccggaagcgc
     1321 tcgcctcgca gaagcaacgt gcgagcgacc tgttccaggc gatgagtccc gactgcctgg
     1381 aggctcggga gaagttgctg aacctgcagc gtccgacggc caggagcgct ccgtcggaca
     1441 tcgagaagca gcaggcgtgg gtcaccgcgc actgcccgga cgtgagccgc accggtgtgt
     1501 ggctcgccga ctggcggtat cctcagcaga agcgctgagg cgtcatggcg agcgttccag
     1561 cgcgcgctat cggtcagtgc gcagcgactg cgcgtcagcg cagtccgggc ggcgcggctt
     1621 caacgcgcca ctctggtagg cgagctcgag cgcggccttg agccacgcga tgcgttgctc
     1681 gggagtgcgg cgctcgaatg accatcggcg gagggcctcg gcgtcgtcga agctgcccca
     1741 ggcgctgggt tcctggcctg gatcgaagcg tttcatgccg tcattctgcc agcatcgact
     1801 tgagtcgatc gatgtccgcc tggtcctgcg gccggccggc agcctccttg agttggatca
     1861 ggtgtccgat cgacgcgatg ggcaccctgc atccaccgat gtcgaccaac gaggcggcag
     1921 cccacagttc cgcgaacggg acggcgggtt ccagcatgac gtcgaccgtc ggtcgcaggt
     1981 tcgtggaatc ccagaagctg aacacctgca tgtggcgcgt cgcctgccat tcgcgacgct
     2041 gctcggggtc ggcgagtcgt gccgggtcga ccggggccag cggcttatag cccgcggccg
     2101 tgagcgcgcg tacgacatcg agcgtggact cgggcgagag atcgatgacg atatccacgt
     2161 ccgcggtcag gcggtcgagc ccgtgaagca cgagcgccag gccaccgacg acaacgaacc
     2221 gcactcgcgc gccggcaaga accgcgaaca gcgcaatgaa atcggtcggc atggcgcatt
     2281 gtcgccccgg agttcctcgc gcgccagctg cagcgacggc gtgccatgca gtagtctgtg
     2341 aagcaggcgg cgaccagggg agcggggcag tgacggacta cgagcgactc aaggaacgac
     2401 aacgcgccga gcggcacgcg cacccggaga gcctggcgct gcgggtccat cgggcgctca
     2461 gctggctgca ccgcgccgag cagctgggga tcaacgacga cctcgacggg cagttcatct
     2521 tcctgtggat cgcgttcaac gcggcctacg cgacggacat cgacgagaag taccgcgatt
     2581 ccgagcagca gacattccgg gcctttctcg aaaagctgac ggcactggac cagggcaagc
     2641 gccgcttcga gctcctgacc tggcaggaat tcccgaagag catccgggtc ctgctcgaca
     2701 acccgtacgt gttcgcggac ttctggaaat tccagagtgg cgcgctgggc gaggacgagt
     2761 ggaggcgcaa cttcgatacc gcgaatcacg ccgcgcgcaa ggcccttggc agccaggaca
     2821 ccgtgtcggt gctcgcgatc gtgctggcgc gcatctacac gctgcgcaac cagctcgtgc
     2881 acggcggcgc gacctggcag agctcggtga accgcgagca gctccgggac tgcacgagct
     2941 tcatggcgaa gctggtgccg ctcgtgatcg aggtgatgat ggaccacccc gacacgctgt
     3001 gggggcaggg gacgtatccg gtggtgaggt agcagcgggg gcggttggtc gcgttcttca
     3061 ggcggatgcg ccagcagctg ctgtccgagc gcaggctcgg cgactacctg cgcnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnntcgcgca gtacgacgca ctgacgcacc
     3241 acctcgacca ggactacgaa gacgactccg aggtcatcac gcaggcgcgc gcctccgtga
     3301 accggttgcg caacatgaac tacccgggca tcgagcgcgt gctggcccac ttcgccggca
     3361 tcccggacga aggttacgag aaggcgttct tcgccttccg cgacaccgac ctgttccgcg
     3421 acacgctgcg tccgcgcgtc gagatcgaat atccgcgcct gcgccggatg gcggacgaca
     3481 tcaccgccag ggtcggtaac gggtatcggt gagccgcgct gatccgacga ggtgtcgccg
     3541 acgtcctcag tgcggagtga agcgcttcac gccagccttg cgcgccgact tcgacagatc
     3601 ggcatccagc gtggcgatcg gcgcaccggc ccgaagcgcg agctcgagat aggacgcatc
     3661 atacgaggag agcgcgtacc ggcgagccac atgcaacgtg tccgtcagcg cgcgggcaaa
     3721 ggtcgcgtca tccggctgta tcgcgacacc ggcgagcatc tcgaggaagg cctcgctctg
     3781 cgcctcatcg acaaggcccc tggcctcggc gcgtaccacg acgttgccga tctcgagcgc
     3841 ccaggtcatg gggacgagcg ccgtgcagtc cggctgccgc agcgagtcga gcacggctgc
     3901 cgcataggca tcgtcggcgg gcctggcgtc accaagcagc cacgacatcg tcaccgaagc
     3961 gtcgagcacg aaggcgctca cgcgcggcct tcctcgatca gcgcccgcac acttgcccgc
     4021 cgccttgcct tgagcgggtg ctcgcgcatg aaggcccgga accggtcgat ggccgtcgcg
     4081 gcgtcggccg atatggcggc cgtgctcggc acgaggtcgg cgaccgcttc gccgcggttg
     4141 gtgatcgtga agcgccgccc ggcgcgcact tcgcgcagca gctccggcaa gcgggtcttg
     4201 gcctcgtagg cgccgatctc ggtcgttacc gtcatggatc ttttcacagc aactagtcta
     4261 tcgactggtc taatggcgtg caacgcgggc acggcacgcc acgggcagcc ggccactcgc
     4321 gcccattcaa cgggtaccgg ccccggtctc gcctgctgaa tatggggcgg ctttgcacgg
     4381 aaccgcgcat gcgggcgttg gagcctttgc ccatatcaac cggccgccac cgcgcagttt
     4441 cagctgaaac gcaaaaatgg cggaattccg ccactttttc cgtcgcactg caaaatcgat
     4501 ttcgacccgc aggtcttcta tccgcccgac cagttcgccg cgaacttgca ccacgcgcac
     4561 tcgggcccgg gatccggcag ttgccccgac agggcgagtg acaccgcgtc gcgaaatgcc
     4621 gcggccaccc acgagcgatt gccgtcgtac ggcacgagcc gcgtgtcgaa gcgcagcgtg
     4681 tcgtggaaac cgtcggcggt cttgaggccg ttggcataga cgaaccacgc gcggtcgctc
     4741 acctccagcc cctgctgcgc gaccaggaac tggtagacgt ccgcctggcg ccggtatgcc
     4801 gggtgcaggg tcgcgctagt gacgtgctcg gccttcgacg tcgccttgta gtccgcgacc
     4861 atgacgcgcc cgtctgcgcc gagccagaga tcgtccacgg cgccgtacag cgtccagccg
     4921 gtgtcggggt ccagccagcg cacgccggtg aagttgtgtc gccactgcgg caggcgcgag
     4981 tcctgcaacg ggaccgcgtc gagcccgacc gtggcgaaga gcgggtgcgg ctcctgtcgc
     5041 gcccgatgcg cgtcgaactc acgcttgaag agctcgtcga ccgcgatgtt caaggtgaat
     5101 gccgggccgc ccgggcgacc gttgtggcgc acgaggtcct cgtagaaaca gcgcggacac
     5161 tcgaggaaca ggtcgagctt cgagcgggag agctggcggc ggtcgaagga ggtcctgccc
     5221 atcgcatcat gttccgggag cgtcggtcgt gccgaagatc tcgttcccct ggcccgaggt
     5281 cttcggccgg cacggccgtc cgtcgtaccg ggcgatctcc gcgcacatcg ccctggcctt
     5341 gaagagggtg agcgtccacc actgctgctg cgcgacctgg tcggcattgc gcatgatggc
     5401 gtagtcgacg aatgccttgt cggcgctcag tgcgtggaag tcgaagtcgg tgcgcacgcc
     5461 gcgaaactcc ttgcgatcgt acgagagccg gatccccttg aagtcctgca gtcccgggcc
     5521 cgccgtgatt ccctgccgga cgtagtcgat ctgcccctgc aggaacagga ggtaggcgta
     5581 gtagtcggcg acggcgtcgc gcagcttcgc gtcaccgatc tcggcgaaca ggcccgtgct
     5641 gatcagttcg tcgtagaccg agcggggagg cgaggcggcc ggcgccaggt cgatcgagcc
     5701 gaaggcctcg ctcacctttt ccgtatcggc cccggcccag tcgtcgtcgc cgagccgttg
     5761 cagggcgtct tcgcgcagcg cgttgtcctt ggcgaacatc gtggcacgct ccgtgatgta
     5821 gtcgacgacc gcctcgctct cggcgtgcag gcgttcgagc gcggccgctt ttcgccgcga
     5881 cagctcggct tctttcttcc aggtcgtcac ctggttggcg atgaacacgc cgaccacgac
     5941 gatgacgaaa tcgatgccga tggcagtcca gtgctgccgt ttcacgtgct cgatgatgcg
     6001 ggacaggatc acggggcgcg gctcctcgag tagcgatggc cggcgtcgcg gcgcgccgga
     6061 aggataaggc tgcggtttac cgggcgcgac ccgcccggcc gcatcgaaat gataatcgtt
     6121 gactttcccg cgaccaggcg tctaatgggg cccgcggcta cccggcgcgt cagcgcggag
     6181 gccagtgccc ggcgcttgcg tgcatggatc acggccgcca ggaggttgtc atgagtcgca
     6241 tcctcgcctt cctctacggc gtggtctgct acgcggtctt cttcgcgacg ttcctgtacc
     6301 tcatgggctt catcgccggc gcctacgtgc cgaagggcat caacgacggc atcgtgaccg
     6361 cgccgatcgt ggccatcgtc atcgatctcg cgctggtcgc tgccttcggc gtgcagcact
     6421 cggtcatggc tcgcccgggc ttcaagtcgt ggctgacgcg cttcgtgtct cccgccgtgg
     6481 aacgcagcac cttcgtgctg gcggcgagcc tcgtgctcgt cctgctctac tggcagtggc
     6541 gcccgctgcc cacggtcgtc tggtcggtgt cggaccccac cgcagtcgag ttgttgtggg
     6601 tgctgtacct ggccggattt ggccttgtcc tggccgcgag cttcgtgatc gaccacttcg
     6661 acctgttcgg gctgcggcag gtctggctgc acctgctgcg acgccagtac gtgcacccgc
     6721 agttcacggt gacctggatc taccggttcg tgcgtcaccc gatctacctc gggttgctgc
     6781 tggcgttctg gagcgcgccc accatgacgg tcggccggct ggtgttcgca ctcgggatgt
     6841 ccgtctacat cctgatcggc atccgctacg aggagcgcga cctggtgcac ttcatcggcg
     6901 aggactatcg gcgctaccgc gagcgcgtgc cgatgctggt gccgcggccg ggtgcgaccc
     6961 acgaagtcgt gaagcccgcg cgagtcgggg acgcctgacg catcaatcgt caggtttcag
     7021 cagcgacgcc agctccgcga acggggtgtg ggtggcgccg ggggcggacg cactgccatc
     7081 ggtcagcggt cgcatgtcga gctggcgctg gtgctcgttg tcgtggcaat agaggcagag
     7141 cagctcccag ttgctgccgt ccggcgggtt gttgtcgtgg tcgtggtcct tgtgatggac
     7201 cgtgagctcg tggacgttgg tgtgatcgaa ctcgcgcgcg cagcggccgc agatccaggg
     7261 gaagagcttc aaggcctggc cgcggtaggt gcgctcgcgt tcctgccgtg cctgtcgggc
     7321 ctcggcgacg atgcggtcga ggcgtgcgtg gtcgggctcg acccgcttgc gggtggtcat
     7381 gggcggaatt gtggaccgcc gcctgcgtcg aatcgagtgg ccgggtgccg tgcacgacgc
     7441 cgaccacccg tggcccgcgc caccggagcc cgggatcagt acctgagagt ccagccttcg
     7501 aggtgagtgc atgcttccgt ggacgccggc agccgtcccg ccgctggcga cgacgctccg
     7561 ggcggcgtca tcgggcgtat gatttcaaca gcgaggccgc gggcctcgtc acacgcggga
     7621 gagcaccatg gcaaagaaaa aggccgatca cggcgtctat cgcgtcaccg aggtcatcgg
     7681 tacgagccgc aaatcctggg aagatgcggc cgccagcgcg gtcgaagcgg ccgccaagtc
     7741 gctgcgtgac ctgcgcatcg ccgaggtgag caagctcgac atgaagatcg agggcggcaa
     7801 ggtcgtcgcc tatcgcgcgc gcgtgctgct ctcgttcaag tacgaggggt gacgcggccg
     7861 cgctgagagc cgtcgcctcg gtccgcggtc cgtcgttgcg gcacgccccc caggctcatc
     7921 ccctgagccc gtccaccgga aagatgggct caccgatcca ggggggcatc caatgagcgc
     7981 ccaggcttgt ccggtctccg accacgcccg ccgcactgcg cgtcgcgtcg ccgacctgct
     8041 ggcgaacgcg gacgcactgc tcattaccgc gggcagcggc atgagtgcgg agtgcgtgcc
     8101 ggccgacgat cccgaactgc gggagctgca ggcgcgcttc gaggcacagg gacgcagctt
     8161 ccgcgagatg gcgcagccgt accgcttcga ccagcacgcc gacctcgcgt ggggctggta
     8221 cggacaccgg cagcgctggt accgccgcat gcggccgcac gacggctatc gcatcgtgcg
     8281 ctcgtggtgc caggcgatgt cctgcggcag cttcgtcgcg acgaccaaca tcgacggcca
     8341 gttcttcgag gccgggttca ccgactggca ggtgctcgag cgtcacggca gcctgctgca
     8401 ttgccagtgc acgcaaccgt gctgcgacga ggtgtggagc gc
//
LOCUS       DAOMFD010000088         8217 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2783_length_8217_cov_35.083803, whole genome shotgun sequence.
ACCESSION   DAOMFD010000088 DAOMFD010000000
VERSION     DAOMFD010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8217)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8217
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2783_length_8217_cov_35.083803"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="PL152_06570"
     CDS             <1..618
                     /locus_tag="PL152_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HQR48980.1"
                     /translation="VRAGLHQQGFAVDWVRDGAAAEHALDVAAYDLCLLDLGLPRKEG
                     LQVLAGLRRKGSALPVVILTARDAIDDRVAGLDAGADDYVVKPFELAELAARIRAVLR
                     RKGGRASSLIEHLGVALDTATHEVRRNGRPVELSGREFAVLHALMEHPGRILSRAQLE
                     ERLYGWDEEVGSNVVEVHIHSLRRKLGADFIRNVRGVGYRVPPAT"
     gene            615..1940
                     /locus_tag="PL152_06575"
     CDS             615..1940
                     /locus_tag="PL152_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR48981.1"
                     /translation="MNSLRARLLAWLLAAVLLVGVAGGFVLYRNALAEANAFFDYHLR
                     ETAMLLRDQAYGFAPPEGLPQEVPQYDFVVQVWSLDGQRLYLSQPTAALPAATTLGFS
                     TVDSRSGRWRVFGILARGHVIQVAQALKVREERAARLALSTLKPFGVLVPVLALLIWW
                     IVGWSLRPLGALADSVRARSPSALDPLPTDRLPDEVRPLVDALNDLLSRLGAALQHER
                     AFIADAAHELRSPLTALSLQLQALAAAGSDEERHEAAMRLRAGVVRATRLVEQLLTLA
                     RHQRAPAAADAPVSLDDVVRGIVSELLPLADAKRIDLGVTRADPAVALGDSDGLHVLV
                     RNLVDNAIRYTPEGGRVDVAVLHEASGGVSRPVLEVCDTGPGIPPDERERVFDRFYRL
                     AGATAAGSGIGLAIVKAIAEQHGARVELSAGAAGRGLRARVVFPGPSAA"
     gene            2025..3545
                     /locus_tag="PL152_06580"
     CDS             2025..3545
                     /locus_tag="PL152_06580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HQR48982.1"
                     /translation="MNAQNPLRSPWLAAIAGAASIALPAGALYLYGPAPHAAESAGAA
                     SAVAEHGPQVVAERAAQATNPATAAARYGLPDFTSLVDQYGGAVVNISIQGTVKTSAG
                     MEMPPLPGFGPDSPFQQFFRGMPRNPHESMPVRGEGSGFIVASNGVILTNAHVVANAQ
                     KVTVKLKDRREFEAKVLGQDEASDVAVIKIDAKDLPTVRLGDPRKVKVGQWVVAIGAP
                     FGFDNSVTAGIVSALGRNLPDDSYVPFIQTDVAVNPGNSGGPLFNLDGEVVGINSQIY
                     SHSGAYEGISFAIPIDVAMNVGRQLQASGHVTRGRLGVGIQDVNQALAESFGLDAPRG
                     ALVSKVESGGPADKAGLKAGDVILAFGDQPVDTAGQLPSIVAAAAPGTSVELKVWRDK
                     SERDVSVKLGEATKDEAVADNGAQSESGRLGLMVRPLSPQEQREADVSQGLVVEDVDG
                     AAAEAGIRPGDIVISANGAPVRSVADLRKVVSGSKDHVALLVQRGDSRIFVPVELG"
     gene            3681..5072
                     /locus_tag="PL152_06585"
     CDS             3681..5072
                     /locus_tag="PL152_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012503.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016831 - carboxy-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-dependent decarboxylase"
                     /protein_id="HQR48983.1"
                     /translation="MTAEKSAPPGPAIAALLEDARRRAVRYLDGLQDRKVAPSAAAVE
                     RLAVLEQPLPAGPTDPAEVLAVLDDIVSPATMAMAGPRFFGFVIGGSLPATLAANWLA
                     AAWDQNTAFANVTPGAAMLEEISLRWLVEFLGLPAATQGAFVTGATVANFTALAAARC
                     EVLARAGWNVEADGLFGAPPVTVVVGEEAHPTLIKSLGLLGLGRNRVVRVPVDGQGRM
                     SARDFPTLSGPAIVCMQAGNINTGSFDPFADIIPRAHEAGAWVHVDGAFGLWAAGVPS
                     LQHLAHGMSDADSWATDAHKWLNVPYDSGLAFVRVTGALPRAMAITAEYLPSGESRRN
                     PADYTPELSRRARGVEVWAALRSLGRSGLVEMFERNCRQAQRFGAALTAAGYEVLNEV
                     VLNQVIVSFGTPEVTGKVIEALQEDGTCWCGVSHWHGRTVMRISVCSWATTDEDVERS
                     IDAMLRVAREVTR"
     gene            complement(5078..5776)
                     /locus_tag="PL152_06590"
     CDS             complement(5078..5776)
                     /locus_tag="PL152_06590"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQR48984.1"
                     /translation="MPRMLARGALAATLPFLATVAMAADPAPLTDDTAKASYALGYQI
                     GLDVQGVEYRPDAVMQGLQDGASGGKAKLTPEEMRAALQKFEQQVQAQKAQRQEEHLK
                     KTAAAGKAYMDQNAKEAGVKTTASGLQYRVIAAGAGKKPAASDTVTVNYRGTLIDGTE
                     FDSSYARGQPATFPLNGVIAGWTEGLQLMQEGGKSELVIPAALAYGDKGPLAGQVLVF
                     EVELIKVGAPEPGK"
     gene            5979..7535
                     /locus_tag="PL152_06595"
     CDS             5979..7535
                     /locus_tag="PL152_06595"
                     /inference="COORDINATES: protein motif:HMM:NF013225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQR48985.1"
                     /translation="MSDQGWHPEIDEIRRRQALAAEMGGEDRVARQRSRGKLTVRERI
                     EALLDPGSFREWGSLAGEGRYGPDGRLESFRASSCLTGRGHIDGRTVVVSADDFTIRG
                     GSSETYHHEKNVQAELMAAEYGLPLVRLLDGTGGGGSVAEVERMGATYVPYVPGFDRV
                     VANLGLVPVVALGLGPVAGLGAARLVASHYSIMVRGLSQMFTAGPPVVAAAGEKVTAD
                     QLGGADLHASTGSVDDTVDSEAEAFARARRFLSYLPSRAGVQPARTECDDPADRAEAW
                     LDAAVPRDRRWPYEIRPIVEAVMDRGSVFEVGRAWGRSVVTSLARLRGVVVAVIASDP
                     GQMGGTWTAAASRKITRHVELAETFGLPIVHFVDVGGFTIGSQAEKAGTIRDGTRAIA
                     TIFQSTVPWCSVILRRAFGVAGAGMMNHTRFRYRVAWPSGDWGSLPVEGGIEAAFKAQ
                     IEAAPDPVAERREIGDRLERLRSPFRSAERFYVEEIIAPSESRRWLCEFAELAAAGPP
                     AGGGRFCFRP"
     gene            7532..8140
                     /locus_tag="PL152_06600"
     CDS             7532..8140
                     /locus_tag="PL152_06600"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HQR48986.1"
                     /translation="MTTSRGLASPLRVLPNLLSSARLVSVPVLAVLAVAGEERVFAWW
                     LVAAMLTDAFDGWIARGFHVKTKLGAFLDSVADSALTLVTVYGVWVFHRDVITGHALL
                     CSLAVGLWALEMLLSLARYGRLSSFHTYLSKASGVLLGIYFGVLFVIGHQPWLFYLAM
                     AVTIAGSLEEILLLAMIREWRADVRGLWWFLRSPPGGRRESR"
BASE COUNT         1122 a         2823 c         2978 g         1294 t
ORIGIN      
        1 gtccgtgccg ggctgcacca gcagggcttt gccgtcgact gggtccgcga cggcgcggcc
       61 gcggaacacg cactcgacgt cgcggcgtac gatctctgcc tcctcgacct cgggctgccg
      121 cgcaaggaag ggctgcaggt cctggccggc ctgcgacgca agggatcggc gttgccggtc
      181 gtgatcctga ctgcgcgcga cgccatcgac gaccgtgtcg ccggtctcga cgccggggcg
      241 gacgattacg tcgtgaagcc gttcgagctc gcggaactcg ccgcgaggat ccgtgccgtg
      301 ctgcgtcgga agggcggccg ggcatcctcg ttgatcgagc acctcggcgt cgcgctcgat
      361 accgcgaccc acgaggtgcg gcgtaacggc cgtccggtcg agttgtcggg gcgcgagttc
      421 gccgtcctgc acgcactcat ggagcacccc ggacggatcc tgtcgcgcgc ccagctcgag
      481 gagcgactct acggctggga cgaggaagtg ggcagcaacg tggtcgaggt ccacatccac
      541 tcgctgcggc gcaagctcgg cgccgacttc atccgcaacg tgcgcggcgt cggctaccgc
      601 gtgccccccg cgacgtgaac tcgctgcgcg caaggctgct cgcgtggctg ctcgcggcgg
      661 tgctgctggt cggcgtggcg ggcggcttcg tcctctaccg caatgcgctc gccgaggcga
      721 acgcgttctt cgactatcac ctgcgcgaga ctgcgatgct gctgcgcgac caggcgtacg
      781 gcttcgcgcc gcccgaggga ctgccgcagg aagtcccgca gtacgacttc gtggtgcagg
      841 tctggtcgct cgacgggcag aggctgtacc tgtcgcaacc gacggccgcg ctgcccgccg
      901 cgaccacgct tgggttctcg accgtggact cgcgctccgg ccgatggagg gtgttcggca
      961 tcctggcgcg cggccacgtg atccaggtgg cgcaggccct caaggtgcgg gaggagcgag
     1021 ctgcgcggct ggcgctctcc acgctcaagc cgttcggcgt gctcgtgccc gtgctcgcgc
     1081 tcctgatctg gtggatcgtg ggctggtcgc tgcggccgct cggcgcactg gcggactcgg
     1141 tgagggcgcg cagcccgtcc gcgctcgacc cgctgccgac ggaccggctt ccggacgaag
     1201 tccgtccact ggtggatgcg ttgaacgacc tgctctcgcg gctcggggcc gccctgcagc
     1261 acgagcgcgc cttcatcgcc gacgcggcgc acgagctgcg ctcgccactg accgcgctca
     1321 gcctgcagct gcaggcgttg gccgcggcag gcagcgacga agagcggcac gaggcggcga
     1381 tgcggctccg cgccggggtg gtgcgcgcca cgcggctggt cgagcagttg ctgacgctgg
     1441 cgcggcatca gcgcgcgccg gctgcagcgg acgcgccggt ctcgctggat gacgtggtcc
     1501 gcgggatcgt ctccgagctg ctgccgctgg cggacgcgaa acggatcgat ctcggtgtca
     1561 cgcgggccga cccagcggtg gcgctcggcg attccgacgg gctccacgtg ctggtccgca
     1621 acctcgtgga caacgcgatc cgctacacgc ccgagggcgg acgcgtcgac gtcgcagtgc
     1681 tgcatgaggc aagcggcgga gtctcccgcc cggtgctgga agtctgtgac accggcccgg
     1741 gcattccccc ggacgagcgc gagcgcgtat tcgatcgctt ctaccgcctg gcgggtgcga
     1801 ccgccgcagg cagcggcatc gggctcgcga tcgtgaaggc gatcgcggaa cagcacggtg
     1861 cacgggtcga actgtcggcc ggcgcagcag gccggggcct ccgggcgcgc gtcgtcttcc
     1921 cggggccgtc cgccgcttaa gcctcgttta agactgcaac ggcagcatcc ttcgtgccac
     1981 gaccgggtgc gtccggccgt gtgcagttca tcggaggaag cagcatgaat gcacagaacc
     2041 cgttgcgcag cccctggctg gccgcgatcg ccggtgccgc ctcgatcgcg ctgcccgcgg
     2101 gtgcgctcta cctgtatggc cctgcgccgc acgcggccga gagcgccggc gccgcatcgg
     2161 ccgtcgccga gcacggtccg caggtcgttg ccgagcgcgc cgcgcaggcc acgaatccgg
     2221 ccacggccgc cgcgcgctat ggtctgcccg acttcacgtc gctcgtggac cagtacggcg
     2281 gcgccgtggt caacatcagc atccagggca cggtgaagac gtccgccggc atggagatgc
     2341 cgccgctccc gggattcggg ccggacagcc cgttccagca gttcttccgt ggcatgcccc
     2401 gcaatcccca cgagtcgatg ccggtgcgcg gcgagggctc cgggttcatc gtcgcgtcga
     2461 acggcgtcat cctgaccaac gcgcacgtgg tcgcgaacgc gcagaaagtc accgtcaagc
     2521 tcaaggatcg ccgcgagttc gaggcgaagg tgctcggcca ggacgaggcg agcgacgtgg
     2581 cggtcatcaa gatcgacgcg aaggacctgc cgacggtgcg cctgggcgac ccgcgcaagg
     2641 tcaaggtcgg ccagtgggtc gtcgcgatcg gcgcgccgtt cgggttcgat aattcggtga
     2701 ccgcgggtat cgtgagcgcg ctcggccgca acctgcccga cgactcctac gtgccgttca
     2761 tccagaccga cgtcgcggtg aaccccggca actcgggcgg cccgctcttc aacctcgacg
     2821 gcgaggtcgt cggcatcaac tcgcagatct acagtcactc gggcgcgtac gaagggattt
     2881 cgttcgccat cccgatcgac gtcgcgatga acgtgggtcg ccagctgcag gcgagtggac
     2941 acgtgacgcg gggccggctc ggcgtcggca tccaggacgt gaaccaggcg ctcgcggagt
     3001 cgttcggcct cgacgcgccg cggggcgccc tggtctccaa ggtcgagtcc ggcggcccgg
     3061 ccgacaaggc gggcctcaag gccggcgacg tgatcctcgc gttcggcgac cagcccgtcg
     3121 acacggccgg gcagctgccg tcgatcgtcg cggccgccgc gccgggcacg agcgtcgaac
     3181 tcaaggtctg gcgcgacaag tcggagcgcg acgtcagcgt caagctcggc gaggcgacca
     3241 aggacgaagc cgtggccgac aacggcgcgc agtccgaatc cggccggctc gggctgatgg
     3301 tgcgtccgct gtcgccgcag gagcaacgcg aagccgacgt gagccagggc ctcgtcgtcg
     3361 aggacgtcga cggtgcggcc gcggaggccg gcatccggcc gggcgacatc gtcatttcgg
     3421 cgaatggcgc gcccgtccgc agcgtggccg acctgcgcaa ggtggtgtcg gggtcgaaag
     3481 accacgtggc actgctcgtg cagcgcggcg actccaggat cttcgtgccc gtcgagctcg
     3541 gctgacgggt tggaatcggg aatcggggac attctcctat ttcggcttcg cgaaatagga
     3601 gaatgtcccc atttccattg gcgcggcgcc ggtcgcggcc gtagcatccc cgccatgcgc
     3661 accgtgattc gcgaggcgtc atgacggcag agaaatccgc accgcccggg ccggccatcg
     3721 cagcattgct ggaggatgcc cggcgacggg cggttcgcta cctcgacggc ttgcaggacc
     3781 ggaaagtcgc gcccagtgcg gcggcggtcg agcggctcgc cgtgctcgag caaccgttgc
     3841 cggccggacc gaccgatccc gccgaggtgc tcgcggtcct cgacgacatc gtgtcgcccg
     3901 ccaccatggc gatggcgggc ccacgcttct tcggcttcgt gatcggcggc tcgttgccgg
     3961 cgacgcttgc ggccaactgg ctcgcagcgg cgtgggacca gaacaccgcg ttcgccaacg
     4021 tcacgcccgg cgcggcgatg ctcgaggaga tctcgctgcg gtggctcgtc gagttcctcg
     4081 ggttgccggc agcaacccag ggcgcgttcg tcaccggcgc gacggtcgcg aatttcaccg
     4141 cgctcgctgc cgcgaggtgc gaggtgctcg cgcgcgcggg atggaacgtc gaggccgacg
     4201 ggctcttcgg cgcaccgccg gtgacggtcg tggtgggcga ggaggcgcac ccgacgctga
     4261 tcaagtcgct aggcttgctc ggtctcgggc gcaatcgcgt ggtccgcgtc cccgtcgacg
     4321 gccagggacg gatgagcgca cgtgacttcc ccacgctcag cggacccgcg atcgtctgca
     4381 tgcaggcggg caacatcaac accgggtcct tcgacccgtt cgcggacatc attccacgag
     4441 cgcacgaggc cggtgcatgg gtgcacgtgg acggcgcatt cgggctgtgg gcagccggcg
     4501 tgccgtcgct gcagcacctc gcgcatggaa tgtcggatgc cgactcgtgg gccaccgacg
     4561 cgcacaagtg gttgaacgtg ccttacgact cgggtctcgc gttcgtgcgc gtgaccggag
     4621 cgttgccgcg ggcgatggcg atcaccgcgg agtacctgcc cagcggcgaa tcacgtcgca
     4681 atcccgccga ctacactccc gagctgtcac gccgcgcgcg tggcgtcgag gtctgggccg
     4741 ctctgcgctc gctgggccgc agcggcctcg tcgagatgtt cgagcgcaac tgccggcagg
     4801 cacagcgctt cggggcagcg ctgacggccg cgggctacga ggtcctgaac gaagtcgtgc
     4861 tgaaccaggt gatcgtgtcg ttcggcacgc ccgaggtgac gggcaaggtc atcgaggcac
     4921 tgcaggagga cggcacgtgc tggtgcggcg tgagccactg gcacggccgc accgtcatgc
     4981 gcatcagcgt ctgctcgtgg gccaccaccg acgaggacgt cgagcgcagc atcgacgcca
     5041 tgctgcgcgt cgcgcgcgag gtcacccgtt aagcgggtta cttgccgggc tcgggcgcgc
     5101 cgaccttgat cagctcgacc tcgaacacca ggacctggcc cgcgagcggg cccttgtccc
     5161 cgtaggccag cgccgcgggg atgacgagct ccgacttgcc gccttcctgc atcagctgca
     5221 ggccctcggt ccagccggcg atcacgccgt tgagcgggaa cgtcgcgggc tggccgcgcg
     5281 cgtaggagct gtcgaattcc gtgccgtcga tcagggtgcc gcggtagttc accgtcacgg
     5341 tgtcgctggc cgcgggcttc ttgccagccc ccgccgcaat cacccggtat tgcagtccgc
     5401 tggccgtggt cttcacgccg gcttccttgg cattctggtc catgtaggcc ttgccggcgg
     5461 cggccgtctt cttcaggtgt tcctcctgtc gctgagcctt ctgcgcctgg acctgctgct
     5521 cgaacttctg cagcgccgca cgcatttcct ccggcgtcag cttcgccttg ccgcccgagg
     5581 cgccgtcctg cagtccctgc atgaccgcat cgggccggta ctccacgccc tggacgtcga
     5641 gcccgatctg gtagccgagc gcatagctcg ccttcgccgt gtcgtcggtg agcggcgcag
     5701 ggtcggcggc catggcgacg gtggccagga agggcagggt cgcggcgagc gcgccacggg
     5761 caagcatgcg gggcatcggg agggctccag gctggggcga caagggcgcg catgttacgc
     5821 gcggtgcgga cattccgcac gttccgcatg ggaccggcgg cgtgtcacgg aagttcctgc
     5881 gcgccggggc gcgggtgtcc cgacttgcca agctgcggct tcctgcgtca ctttgccgga
     5941 cccgggccgg ccacgcccga ggccgccgga ggaatcgcat gagtgaccag ggctggcatc
     6001 cggaaatcga cgagattcga cgccggcagg cgctggccgc cgagatgggc ggcgaggacc
     6061 gcgtcgcgcg acagcgatcg cgcggcaagc tcacggtgcg cgagcgcatc gaggcgctgc
     6121 tcgacccggg ctcgtttcgc gagtggggca gcctcgcggg cgagggccgg tacggtccgg
     6181 acggccggct cgagtcgttt cgcgcgtcga gctgcctcac cggccgcggc cacatcgacg
     6241 gccgcaccgt ggtcgtgagc gccgacgatt tcacgatccg gggcggatcg tccgagacct
     6301 accaccacga gaagaacgtg caggccgagc tcatggccgc cgagtacggc ctgccgctgg
     6361 tgcgcctgct cgacggcacg ggcggcggcg gatccgtcgc cgaggtcgag cgcatgggtg
     6421 cgacctacgt gccgtacgtg cctggattcg atcgggtcgt cgccaatctt ggcctggtcc
     6481 cggtcgtcgc gctgggcctg ggaccggtgg ccggcctcgg cgcggcgcga ctcgtcgcga
     6541 gccactactc gatcatggtg cgcgggctga gccagatgtt caccgccggc ccgcccgtgg
     6601 tcgccgcggc cggcgagaag gtcacggccg accagctcgg cggcgcggac ctgcacgcat
     6661 cgaccggcag cgtggacgac acggtggaca gcgaagccga ggcattcgcg cgcgcgcgcc
     6721 gattcctctc ctacctgccg agtcgcgccg gagtgcaacc cgcgcgcacg gagtgcgacg
     6781 atccagcgga tcgcgcggag gcctggctcg acgcggcggt gccgcgcgac cggcgctggc
     6841 cctacgagat ccgcccgatc gtcgaggccg tgatggatcg cggcagcgtg ttcgaggtcg
     6901 gccgcgcctg gggtcgttcg gtggtgacgt cgctggcgcg cctgcgcggc gtggtcgtcg
     6961 cggtgatcgc gagcgatccc gggcagatgg gcgggacgtg gactgcggcg gcctcgcgca
     7021 agatcacccg ccacgtggag ctcgccgaga ccttcggtct gccgatcgtg cacttcgtcg
     7081 acgtcggcgg cttcacgatc ggctcgcagg cggagaaggc cggcacgatc cgcgacggca
     7141 cgcgggcgat cgcgacgatc ttccagtcca ccgtgccgtg gtgctcggtc atcctgcgcc
     7201 gcgcgttcgg ggtcgctggc gcgggcatga tgaaccacac gcgcttccgc tatcgcgtcg
     7261 cctggccgag cggcgactgg ggctcgctgc cggtggaagg cggcatcgag gcggcgttca
     7321 aggcgcagat cgaagcggcg ccggaccccg tcgccgagcg tcgcgagatc ggcgaccggc
     7381 tcgagcgcct gcgctcgccg tttcgttcgg ccgaacgctt ctacgtggag gaaatcatcg
     7441 cgccgtcgga gtcgcggcgc tggctctgcg aattcgcgga actcgccgcc gcaggtccgc
     7501 ctgccggcgg tggccggttt tgcttcaggc catgaccaca tcgcgcgggc tcgcgtcgcc
     7561 cctacgcgtc ctgcccaacc tcctgagcag cgcccgactc gtgtcggtgc cagtgctggc
     7621 cgtgctcgcc gtcgcaggcg aggagcgcgt cttcgcgtgg tggctggtgg ccgcgatgct
     7681 caccgacgcc ttcgacggct ggatcgcgcg cggattccat gtcaagacga agctcggcgc
     7741 cttcctcgac tcggtcgcgg actcggcgct gacattggtc acggtctacg gggtctgggt
     7801 ctttcaccgc gacgtcatca ccgggcacgc actgctctgc agtctcgcgg tcggcctgtg
     7861 ggcgctcgag atgctgctct cgctggcgag gtacggccga ctgtcgagct tccacacgta
     7921 cctctcgaag gccagcggcg tgctgctggg gatctatttc ggcgtgctgt tcgtgatcgg
     7981 ccaccagccg tggctctttt acctggccat ggccgtgacg atcgccggca gcctggagga
     8041 gatcctgctg ctggccatga tccgagagtg gcgagccgac gtccgggggc tgtggtggtt
     8101 tctgcgcagt ccgccgggcg ggcgtcgaga atcccggtaa aaacaggcac ttattggctg
     8161 cccggtcggg cgcggccgga cgtccctggc ttgcggaatt tgcggtgtcg acgacca
//
LOCUS       DAOMFD010000089         8174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2809_length_8174_cov_35.983372, whole genome shotgun sequence.
ACCESSION   DAOMFD010000089 DAOMFD010000000
VERSION     DAOMFD010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8174
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2809_length_8174_cov_35.983372"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..410)
                     /locus_tag="PL152_06605"
     CDS             complement(<1..410)
                     /locus_tag="PL152_06605"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HQR48987.1"
                     /translation="MSTGKLVLLRHGQSIWNLENLFTGWIDVDLSDQGLLEAAEAGRL
                     LRAEGIAFDIAFTSVLKRAIRTLWIALDEMDLMWLPVERTWRLNERHYGALQGLDKAK
                     TVERHGAEQVKIWRRSYDIPPPPLANDDPQNPRF"
     gene            complement(407..5227)
                     /locus_tag="PL152_06610"
     CDS             complement(407..5227)
                     /locus_tag="PL152_06610"
                     /EC_number="1.4.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF016944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004352 - glutamate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0019551 - glutamate catabolic process to
                     2-oxoglutarate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-glutamate dehydrogenase"
                     /protein_id="HQR48988.1"
                     /translation="MAGSIPAARKHLIDLIAAQARPAQPKTPVPPAHFARDYYRGVDE
                     DDLRARTPAALAAAATDHLRFGTKRRAGQPLVRVFNPTQLQHGWTSPHTVVEVVTDDM
                     PFLLDSLAMVLNDCGLALHMMAHPVLYVTRDRAGRIKTCSGEAAKGARAESWQHIEMD
                     GVADAARLEDVRRRILATLADVELAVRDWAAMRTRATDIAAEIAVGVPGIGRAECSEA
                     RAFLAWLADHHFTFLGYREYRLVRGASADKLVPVTGSGLGLLRSGKGRITPKPVTLRE
                     ETRRRAREATVLVVTKANSVSTVHRAKYLDYVGVKTFDERGEVTGERRFLGLFTSTTY
                     SASPRDIPLLRHKVQHVIDHIGVAPVSHDGKELFHVLESYPRDELFQASVPELVRSAR
                     GIVNIYERRRVRLFVRRDPYGRFFSCLLYVPRDRYNTQARQRIEQILLQELAGLSHET
                     QVQISESALARLYTLVRTDPDHEVTADVERIERLVTDALRTWEDRLRDELQARLPGDR
                     PARFAAAFPPAYKDDVPARDAIDDIRELLALPESGTAVGLQLRPGDAVRKALHLRLYR
                     RGEPIAMSDLVPMLENFDLRILNERPYRIGAGHDLWIQDLEVTHAGGRTLDPELVGAR
                     FEDAFFAVWNGQAESDGFNRLVLAAGLHWRQAVVLRAVCRYLLQTGLTFSQGYMESVL
                     TRHPGIAAHLAWTFEARFDPELKDSTRASQLRSLARETDEALEKVTSPDDDRILRAFR
                     AVINATLRTNHYQADPAGNPKPYLSFKLDPKSLPELPKPRPMFEIWVYSPRVEGVHLR
                     MGKVARGGIRWSDRREDFRTEILGLMKAQNVKNTLIVPVGSKGGFFPKQMPRGASRED
                     VQREGTACYQMFIRGLLDLTDNVQGSQVVPPARTVRYDPDDPYLVVAADKGTATFSDT
                     ANALAAEYGFWLGDAFASGGSAGYDHKKMGITARGGWECVKRHFREMGVDTQSQDFTV
                     AGIGDMAGDVFGNGMLLSRHIRLVAAFNHQHIFLDPSPDAEASFAERERLFSLPRSSW
                     SDYNTRLISAGGGVFPRDAKEIALSAQARALLGIDASKATPTQVIRAILLLPVDLLWN
                     GGIGTYVKASWEANSAIGDRTNDAVRVDGRELRCKVIGEGGNLGLSQLGRIEFALNGG
                     RLNTDFVDNSGGVDCSDHEVNIKILLDVVSRRSKLPTAARNRLLAEMTDDVGELVLRD
                     NYLQSQAISLLESTAAERLGEHAHFIRSLELDGVLDRALEFLPSTEEIDERRRTGRGL
                     TRPELSMVLSYAKIALSQQLIQSDVPEDPYLGRELDRYFPDRLAKRYASLLGEHRLRR
                     EIITTATTNSIVNRMGPTFVARTRQDTGADAAAVARAYTIAREAFDVRDIWRAIERLD
                     NKVVAGVQYAMVQDTIALLRQATYWLLQRHRDELGIEMQVGRLRPGIRELSAALPQWL
                     HGLERKAFDARAAELTGAGVPAALARQVAACSALQSAPDIVELARARRLSVDAAAATY
                     FGAGAEFGIDWLRARVEDLHIEGHWHAVARGSLREALYETQRAIAQSVLEESREKEPA
                     RAIERWMKKHEAAATHARGVVGDIREQSSKADFASLAVALQAVRRLVVSEGRTE"
     gene            complement(5324..5803)
                     /locus_tag="PL152_06615"
     CDS             complement(5324..5803)
                     /locus_tag="PL152_06615"
                     /inference="COORDINATES: protein motif:HMM:NF024854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR48989.1"
                     /translation="MKHSAVTSIELERFLPYRLSVLTNLVSSAIAGEYQQRFGLGIPE
                     WRVVAVLARNPGLSAAEVAAYTRMDAVAVSRAVTRLARAGRIRRSVAPDDRRRSVLRL
                     SPAGRAVYRRIAPLALRYERELLECLADGETVALDTIIAKLTSRANALATDGLDDGR"
     gene            complement(5866..>6184)
                     /gene="zapE"
                     /locus_tag="PL152_06620"
     CDS             complement(5866..>6184)
                     /gene="zapE"
                     /locus_tag="PL152_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HQR48990.1"
                     /translation="VAWFDFAELCEGPRGTADYVEIARGFHTVFVSGVPVFDSARDNE
                     ARRFIALVDEFYDRSVKLVLAAAAMPDALYRGERLAFEFDRTRSRLAEMQTHAYLARA
                     HRP"
     assembly_gap    6185..6284
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6285..6631)
                     /gene="zapE"
                     /locus_tag="PL152_06625"
     CDS             complement(<6285..6631)
                     /gene="zapE"
                     /locus_tag="PL152_06625"
                     /inference="COORDINATES: protein motif:HMM:NF015902.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AFG1/ZapE family ATPase"
                     /protein_id="HQR48991.1"
                     /translation="MNDAGSLARAYHESLQRHGYSPDRSQLHAVGKLDALHRRLHSPA
                     PGGWFARMLATLRGPGTEPARGLYLWGGVGRGKTFLMDLFHAELRVPARRDHFHRFMK
                     DVHGRLAGWRHVE"
     gene            complement(6648..>8174)
                     /locus_tag="PL152_06630"
     CDS             complement(6648..>8174)
                     /locus_tag="PL152_06630"
                     /EC_number="6.2.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030729 - acetoacetate-CoA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate--CoA ligase"
                     /protein_id="HQR48992.1"
                     /translation="RVVGFMPNLPETTIAMLATTSLGGVWSSCSPDFGINGVLDRFGQ
                     IAPRVLFTADGYFYNGKTLDSLAPIRGVLERLDTVKRVVVVPYVNPDPDISRLPAAVR
                     FGEFGQPGAPLQFVRLPFDSPLYVMYSSGTTGVPKCIVHGVGGTLLQHRKEHALHSDL
                     KSGDRLFFYTTCGWMMWNWLMSGLASGCTLVLYDGSPFAPDRDVLWRMAEREKVTHFG
                     TSPKYLAALEKDGGAVGSAFDLTSLRAIISTGSPLAPEQYDYVHSTIKRDVHLASISG
                     GTDIVSCFCIGNPCLPVYPGEIQCRPLGMKTQVFDDDGHPLVGREGELVCSAPFPSMP
                     IGFWNDADGSKYRAAYFERYPDVWCHGDYALQTEHGGFVILGRSDAVLNPGGVRIGTA
                     EIYRQVEKVHEVLESVAVGQNWRHDVRVVLFVKLRPGVALDAALEKRIRDTIRANTTP
                     RHVPAKIIAVADIPRTISGKIVELAVRNVIHGRPVKNTDALANPEALEHFRDLAELSD
                     "
BASE COUNT         1218 a         2738 c         2823 g         1295 t
ORIGIN      
        1 tcgaatcgcg ggttctgcgg atcgtcgttc gcgagcggcg gaggcgggat gtcgtaactg
       61 cgccgccaga ttttcacctg ctcggcgccg tgccgttcga cggtcttcgc cttgtcgagt
      121 ccctgcagcg ccccgtagtg ccgctcgttg agccgccagg tgcgctcgac cggcaaccac
      181 atgaggtcca tctcgtcgag cgcaatccac aacgtgcgga tcgcacgttt cagcaccgaa
      241 gtgaaggcga tgtcgaaagc gattccttcg gcgcgcaaca ggcgtcccgc ctcggcggcc
      301 tcgaggaggc cctggtcgga gagatcgacg tcgatccagc cggtgaacag gttctcgagg
      361 ttccagatgc tctggccgtg gcgcagcagt accagttttc ccgtgctcat tccgtccttc
      421 cttccgacac gacgaggcgg cgcaccgcct gcagggcgac cgccagcgac gcgaagtcgg
      481 ccttcgacga ttgctcgcgg atgtcaccca cgacgccccg cgcgtgcgtc gcggcggctt
      541 cgtgcttctt catccagcgc tcgatggcgc gcgccggttc cttctcgcgc gattcctcga
      601 gcacgctctg cgcgatcgcg cgctgcgtct cgtacagcgc ctcgcggagg ctgccgcggg
      661 cgaccgcgtg ccagtggcct tcgatgtgca ggtcctcgac gcgggcccgg agccagtcga
      721 tgccgaactc cgcgccggca ccgaagtagg tggcggctgc tgcgtcgacc gacaggcgcc
      781 tggcgcgggc aagttccacg atgtcgggcg cggactgcag cgccgagcag gcagcgacct
      841 gccgtgcgag cgcggccggc acgccggccc ccgtgagttc cgcggcacgc gcatcgaatg
      901 ccttgcgctc gagaccgtgc agccactgcg gcagcgcggc ggacagctcg cggattcccg
      961 ggcgcagtcg ccccacctgc atctcgatgc cgagctcgtc gcggtggcgc tgcagcagcc
     1021 agtaggttgc ctggcgcagc agtgcgatcg tgtcctgcac cattgcatac tgcacgccgg
     1081 ccacgacctt gttgtcgagg cgctcgatcg cgcgccagat gtcacgcacg tcgaaggctt
     1141 cccgcgcaat cgtgtaggcg cgcgccaccg cggccgcgtc ggcaccggtg tcctggcgcg
     1201 tgcgtgcgac gaacgttggc cccatgcggt tgacgatgct gttggtcgtc gcggtggtga
     1261 tgatttcccg cctcaggcgg tgctcgccga gcagggacgc gtagcgcttg gcgagccggt
     1321 ccgggaagta acgatccagc tcacggccga ggtacgggtc ctccggcacg tcggactgga
     1381 tgagctgctg gctcagcgcg atcttggcgt agctcagcac catcgacagc tccggccgcg
     1441 tgagcccgcg cccggtgcgc cggcgctcgt cgatctcctc ggtgctcggc aggaactcga
     1501 gcgcacggtc gagcacgcca tccagctcga gcgagcggat gaaatgcgcg tgctcgccga
     1561 gccgctcggc cgcggtcgac tcgagcaggc tgatcgcctg gctctgcagg tagttgtcgc
     1621 gcagcacgag ctcgcccacg tcgtcggtca tctcggcgag caggcggttg cgtgccgcgg
     1681 tcggcagctt gctgcggcgg ctcaccacgt cgagcaggat cttgatgttg acctcgtggt
     1741 ccgagcagtc cacgccgccg gagttgtcga cgaagtccgt atttagacgg ccgccgttca
     1801 gggcgaactc gatgcgcccg agctgcgaca gcccgaggtt gccgccttcg ccgatcacct
     1861 tgcagcgcag ctcgcggccg tcgacgcgca cggcgtcgtt ggtgcgatcg ccgatcgcgg
     1921 aattcgcctc ccacgacgcc ttgacgtacg tgccgatgcc gccgttccac agcaggtcga
     1981 ccggcagcag caggatggca cggatcacct gtgtcggcgt cgccttggat gcgtcgatgc
     2041 cgagcagtgc gcgggcctgc gcactcagcg cgatttcctt cgcatcgcgc gggaatacgc
     2101 cgccacccgc ggagatcagg cgagtgttgt agtccgacca cgacgagcgc ggcaggctga
     2161 agagccgctc gcgctccgca aagcttgcct ccgcgtcggg cgatggatcc aggaagatgt
     2221 gctggtgatt gaacgccgcg accaggcgga tgtgacgcga cagcagcatg ccgttgccga
     2281 acacgtcgcc cgccatgtcg ccgatgcccg ccaccgtgaa gtcctgcgac tgcgtgtcca
     2341 cgcccatctc gcggaagtgg cgcttgacgc actcccagcc gcccctggcg gtgatgccca
     2401 tcttcttgtg gtcgtatcca gcggacccac cggacgcaaa ggcatcgccc agccagaatc
     2461 cgtactccgc cgcgagcgca ttggccgtgt cggagaacgt cgccgtgccc ttgtctgccg
     2521 cgacgaccag gtacgggtcg tcggggtcgt agcgcacggt gcgcgccggg gggacgacct
     2581 ggctgccctg cacgttgtcg gtgaggtcga gcaggccacg gatgaacatc tggtaacacg
     2641 ccgtcccttc gcgctggacg tcctcacggc tcgcgccgcg gggcatctgc ttcgggaaga
     2701 agccgccctt cgagcccacc ggcacgatga gcgtgttctt cacgttctgc gccttcatga
     2761 gaccgaggat ctcggtgcgg aaatcctcgc gccggtcgga ccagcggatg ccgccgcggg
     2821 cgaccttgcc cattcgcagg tgcacaccct cgacgcgtgg cgaatacacc cagatctcga
     2881 acatcggccg cggcttcggc agctccggga ggctcttcgg gtcgagcttg aacgacaggt
     2941 acggcttcgg gttgcccgcc gggtccgcct ggtagtggtt cgtgcgcagc gtcgcgttga
     3001 tcacggcgcg gaacgcacgc aggattcgat cgtcgtcggg gctcgtgacc ttctcgagcg
     3061 cctcgtcggt ctcgcgggcc agggagcgca actgcgaggc gcgggtcgag tccttgagtt
     3121 cggggtcgaa gcgcgcctcg aaagtccacg cgaggtgcgc cgcgatgccc gggtggcgcg
     3181 tgagcaccga ctccatgtac ccctggctga acgtcaggcc ggtctgcagc aggtagcggc
     3241 agacggcgcg cagcacgacg gcctgtcgcc agtgcagccc ggccgcgagc accaggcggt
     3301 tgaatccgtc gctctcggcc tggccgttcc agaccgcgaa gaacgcgtcc tcgaatcggg
     3361 cgcccacgag ttccggatcc agcgtgcggc cacccgcgtg cgtgacctcg aggtcctgga
     3421 tccagaggtc gtggcccgcg ccgatccggt acggccgctc gttcaggatc cgcaggtcga
     3481 agttctccag catcggcacc aggtccgaca tcgcgatcgg ctcgccacgc cggtacaggc
     3541 gcaggtgcag cgccttgcgg acggcatcgc cgggccgcaa ctgcagcccg accgccgtgc
     3601 cggactcggg aagcgccagc agttcgcgga tgtcgtcgat ggcatcgcgc gccggcacgt
     3661 cgtccttgta tgcgggagga aacgccgcgg cgaaccgtgc tggccggtca ccgggcaacc
     3721 gcgcctgcag ctcgtcgcgc agccggtcct cccaggtgcg cagcgcatcg gtcaccagtc
     3781 gctcgatccg ttcgacgtcc gcggtcacct cgtgatcggg atcggtgcgc accagcgtgt
     3841 acaggcgcgc gagcgcggat tccgagatct ggacctgcgt ctcgtgggac aggcccgcca
     3901 gttcctggag caggatctgc tcgatgcgct gcctggcctg cgtgttgtag cggtcgcgcg
     3961 ggacgtacag caggcagctg aagaagcggc cgtaggggtc gcggcggacg aacagccgca
     4021 cgcgccggcg ctcgtagatg ttgacgatgc cacgggcgct gcgcacgagc tccggcacgc
     4081 tcgcctggaa cagctcgtcg cgcgggtagc tctcgaggac gtggaacagc tccttgccgt
     4141 cgtggctcac cggtgccacg ccgatgtggt cgatcacgtg ctggaccttg tggcgcagca
     4201 gcgggatgtc gcgcggactg gcgctgtagg tcgtggacgt gaagagcccg aggaatcgcc
     4261 gctctcccgt cacctcgcca cgctcgtcga aggtcttcac gccgacgtag tcgaggtact
     4321 tggcgcgatg gaccgtggac accgagttcg ccttggtcac gacgagtacc gtcgcttcgc
     4381 gcgccctgcg gcgagtctct tcgcgcagcg tcaccggctt cggtgtgatt cgtcccttgc
     4441 cgctgcgcag cagtcccagg cccgagccgg tgaccggcac cagcttgtcg gccgacgcgc
     4501 cgcgcaccag tcggtactcg cggtagccga ggaaggtgaa gtgatggtcg gcgagccacg
     4561 ccaggaaggc cctggcctcg ctgcattcgg cgcggccgat gccgggtacg cccacggcga
     4621 tttcggccgc gatgtcggtc gcgcgggtgc gcattgccgc ccagtcgcgg acggccagct
     4681 cgacgtcggc gagcgtcgcg aggatgcggc ggcgcacgtc ctcgagacgt gccgcgtcag
     4741 cgacgccatc catctcgatg tgctgccacg attcggcgcg cgcgcccttg gcagcttcgc
     4801 ccgagcaggt cttgatgcga ccagcccggt cacgcgtcac gtacaggacc gggtgcgcca
     4861 tcatgtgcag cgcgaggccg cagtcgttga gcaccatcgc cagggaatcg agcaggaacg
     4921 gcatgtcgtc ggtcacgacc tcgaccaccg tgtgcggcga cgtccagccg tgctgcagct
     4981 gcgtcgggtt gaacacccgc accagcggct ggccggcgcg ccgcttcgtg ccgaagcgca
     5041 ggtgatcggt cgcggcggct gccagggcgg cgggggtgcg ggcgcgcagg tcgtcctcgt
     5101 ccacgccgcg gtaatagtcc ctggcgaagt gcgcgggcgg gaccggcgtc ttcggctgcg
     5161 cagggcgggc ctgggctgcg atcaggtcga tcaggtgctt gcgcgcggcc gggatgctgc
     5221 ctgccatgtt gttcttcctc agttccgatt gccgccgtgg gcggaattat aggccacggc
     5281 cgcccgcggc tcggatgccg atgcatccgc acaacccgca acgtcaacgg ccatcatcga
     5341 ggccatccgt cgcgagcgcg ttggcccgcg aggtgagctt ggcgatgatc gtgtcgaggg
     5401 ccacggtttc gccgtctgcc aggcattcga gcagctcgcg ctcgtagcgc agtgcaagcg
     5461 gggcaatgcg ccgatagacg gcgcgacccg ccggcgacag acgcagcacg gagcgacggc
     5521 gatcgtccgg cgccaccgag cggcggatcc ggccggcacg cgccagtcgc gtcaccgccc
     5581 ggctgaccgc gacagcgtcc attcgcgtgt aggcggccac ttcggctgcg gacagccccg
     5641 ggttcctggc gagaacggcg accacccgcc actccgggat gccgagcccg aagcgctgct
     5701 ggtactcgcc cgcgatcgcg ctgctcacga ggttggtgag caccgacagc cggtagggca
     5761 ggaaccgctc cagctctatg gacgtcaccg cactgtgctt cacggcgtct cccgccgacc
     5821 cgatgtttgc aagtgtaacg attcggggcg cagccgtgcc atgcactagg ggcggtgcgc
     5881 ccgcgcgagg taggcatggg tctgcatctc ggcgagccgg ctgcgcgtgc ggtcgaattc
     5941 gaaggcaaga cgctcgccgc ggtacagggc atccggcatg gccgcggcgg ccagcaccag
     6001 cttgaccgac cggtcgtaga actcgtcgac gagtgcgatg aaccggcgtg cctcgttgtc
     6061 ccgtgccgaa tcgaaaaccg gtactcccga gacgaagacc gtgtggaaac cgcgcgcgat
     6121 ttccacgtag tcggcggtac cgcgcgggcc ctcgcagagc tcggcgaagt cgaaccacgc
     6181 gacgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntcctcg acgtgccgcc
     6301 acccggcgag gcgcccgtgc acgtccttca tgaagcgatg gaagtgatcg cgccgggcag
     6361 ggactcgcag ctcggcgtgg aacaggtcca tgaggaacgt cttgccgcgc ccgacgccac
     6421 cccacaggta aaggccgcgt gcgggctccg tgcccgggcc gcgcaacgtg gcgagcatcc
     6481 tcgcgaacca gccgcccggc gccggcgagt gcagtcggcg atgcagcgcg tcgagcttgc
     6541 cgaccgcgtg gagctgggag cggtccgggg agtacccgtg gcgctgcaac gattcgtggt
     6601 aggcgcgggc caggcttccg gcgtcgttca tcgggccggg cgcggtgtca gtcggacagt
     6661 tccgccaggt cgcggaagtg ctcgagcgct tccggattgg cgagcgcgtc cgtgttcttg
     6721 accggacggc cgtggatcac gttgcgcacg gcgagttcca cgatcttgcc gctgatggtg
     6781 cgcgggatgt ccgccaccgc gatgatcttc gccggcacgt ggcgcggcgt cgtgttcgcc
     6841 ctgatcgtgt cgcggatgcg cttctccagc gccgcgtcga gcgccacgcc ggggcgcagc
     6901 ttcacgaaca ggacgacgcg cacgtcgtgc cgccagttct ggccgacggc cacgctctcg
     6961 agcacctcgt gcaccttctc gacctgccgg tagatctcgg cggtgccgat gcgcacgcca
     7021 cccgggttca gcacggcgtc ggagcggccg aggatcacga atcccccgtg ctcggtctgc
     7081 aacgcgtagt cgccgtggca ccagacgtcc ggatagcgct cgaaatacgc ggcgcggtac
     7141 ttgctgccgt ccgcgtcatt ccagaacccg atcggcatgg acgggaaggg cgcgctgcag
     7201 acgagttccc cctcgcggcc gaccagagga tgaccgtcgt cgtcgaatac ctgcgtcttc
     7261 atgccgagag gacggcactg gatctcgccg ggatagacgg gcaggcacgg gttgccgatg
     7321 cagaagcacg acacgatgtc cgtgccgccg gaaatggacg cgaggtgcac atcgcgcttg
     7381 atggtcgagt ggacgtagtc gtactgctcg ggcgcgagcg gcgaaccggt cgagatgatc
     7441 gcgcgcagcg aggtgaggtc gaacgcgctg ccgaccgcgc ctccgtcctt ctcgagtgcg
     7501 gcgaggtact tgggactggt gccgaagtgc gtgaccttct cgcgctcggc catgcgccac
     7561 agcacgtcgc ggtccggcgc gaagggcgag ccgtcgtaca gcacgagcgt gcagcccgag
     7621 gcgagccccg acatgagcca gttccacatc atccagccgc aggtcgtgta gaagaacagc
     7681 cggtcgccgg acttgaggtc gctgtgcagc gcgtgctcct tgcgatgctg cagcagcgtc
     7741 ccgccgacgc cgtgcacgat gcacttcggc acgccggtgg tgccggacga atacatcacg
     7801 tagagcggcg agtcgaaggg caatcgcacg aactgcagcg gtgcacccgg ctggccgaac
     7861 tcgccgaacc tcaccgcggc cggaagccgg gaaatgtccg ggtcggggtt gacgtacgga
     7921 acgacgacga cccgcttcac ggtgtccagc cgctcgagca cgccccggat cggcgcgagc
     7981 gagtcgagcg tcttgccgtt gtagaaatag ccgtcggccg tgaacagcac gcgtggcgcg
     8041 atctgtccga agcggtcgag cacgccgttg atgccgaagt ccggggagca cgaggaccac
     8101 acgccgccga ggctggtcgt ggcgagcatc gcgatcgtcg tctcggggag attgggcatg
     8161 aagccgacga cgcg
//
LOCUS       DAOMFD010000090         8111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2849_length_8111_cov_33.767130, whole genome shotgun sequence.
ACCESSION   DAOMFD010000090 DAOMFD010000000
VERSION     DAOMFD010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8111
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2849_length_8111_cov_33.767130"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="PL152_06635"
     CDS             <1..404
                     /locus_tag="PL152_06635"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQR48993.1"
                     /translation="YAGVPLTHREHAQSVRFVTAHDHESFAEADWAALSAGRQTLCLY
                     MGIAELASFAARLMAHGRRSETPVAVVENGSRTDQRVTLASLGQLGDLSVRAALQSPA
                     MVIVGEVAALAGTLAWFGEEPRRWESLRRVA"
     gene            complement(433..2550)
                     /locus_tag="PL152_06640"
     CDS             complement(433..2550)
                     /locus_tag="PL152_06640"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR48994.1"
                     /translation="MTDACVPRRDDSSRLRRLLIAAGVLYASHAAALHAEPAGPLEEV
                     VVTATRKPQPALDVPLSIGRVGTDGIVLVDATHHAEILNRIPGVMIQQGSGQESLTAI
                     RSPVLTGAGSCGAFLFLENGVPVRPVGFCNVNALYEVNTEQAQAVEVLRGPGSALYGS
                     NAMFGTVNVIQPAPLDRPPLAASVDAGPSSYWRVKVAGRHDGSAADFGAAGLYTHDGG
                     WRDDSGYDEAKFNATLVGDLGTTPARADLAVTNLDQETAGFITGKNAYRSLELSESNP
                     TPGAFRNSTAVRLTGLLQPRLLDGAELDLRPFLRSSHMDFLQHFLLGQPVEKNGQVSG
                     GLLTSIAWGDVQGWSVIVGADVELGQSSLEEYQDHPTTGGSAAANAIRPAGKHYDYEV
                     RSNVESLYAQAERRFAERWRITAGVRAEWVTYDYDNRMLDGNTDDQGVPCTPAPCLYS
                     RPADRTDHFNDWAPKLSVSYDFTPSLVGYATLTQGFRPPEMTELYRLQRMQRVADLQP
                     EELDSVELGLKGAWSRVSLALAVYDMKRSGVILRESNGYNVDNGRTTHRGIEYDLRWR
                     ALPSLDVVAAGTYAIHRYDFSRRVDGGETLVAGNDIDTAPREIGRYGLEWRPTASVEA
                     EAEWMVVGPYWLDAANAHRYGGYELVNLRGLWRFAPQWCAGLRVTNALDVRYADRADY
                     SFGTYRYFPGEPRAVFAEIAWTM"
     gene            2650..4878
                     /locus_tag="PL152_06645"
     CDS             2650..4878
                     /locus_tag="PL152_06645"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQR48995.1"
                     /translation="MRIRCVLMTACAMLLIAGPGRVMADDAPQEPAASRPKIGLVLSG
                     GGARGAAHIGVIRALEQMHVPIDAVAGTSMGAVVGGLYAAGLSGDEIEQVFRSLDWQE
                     LLRDRAPRRDLVYRRKLDDRSFLATGGLGIRADEGVVLPLGLITGQKITQVLRTSTMR
                     VADVQDFDKLPTPFRGLATDLETGDPVVLRSGDLATVLRASMSAPGVLAPVEIGDRLL
                     VDGGLVDNLPVDLARQMGVDVVIAVDVSFPLARRSGLETPLDVTSQMIAIMVRRGTAA
                     SLANLRPGDVLVTPELGRMTSIDFTKMPQVIVAGETAADQQSAKLASLALPADQYAAY
                     VAARARAADPNVRVAFVRPGPHSEADAKRIDAVFGDLAGKPLDTRELQQRAYSQYALD
                     RYEAVDYRVVNDDGERGLEIDLRRKSWGPNFLRFGMSIESDYEGGTYANAGARLLMTG
                     VNRLDGELLLDGQLGEDPHFYTELFQPLSFKTEFFIAPAFRYDLETLQYVQDGHRLAQ
                     YRVRDTEFLLAVGAELSNWGEVRLGVRRGTGSSRLMVGEPVFPNEEFDLGAAYLEFGY
                     DRLDSAYFPKHGQAFRASWQSESKALGASANADIVEASWVFARSHDRNSLVLSLAGGS
                     ALDDRVVSPQELFTLGGFLNLSGLPQDDLIGTQYGIARAIVYRRISRGGTGLFEFPAY
                     IGASLEAGNVWQTKADVDFGDLKVGGSGFLAAETPFGPLYLAAGFAEGDAALYLVLGK
                     TF"
     gene            4928..5179
                     /locus_tag="PL152_06650"
     CDS             4928..5179
                     /locus_tag="PL152_06650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR48996.1"
                     /translation="MTTCHEHNLRAPLPAARPYGIRVRLRHDDPFRSLLGDDWHREHW
                     FATRAERDRALEEMSGRYVYFRPGDAPALDFERVDPRAA"
     gene            5176..5877
                     /locus_tag="PL152_06655"
     CDS             5176..5877
                     /locus_tag="PL152_06655"
                     /inference="COORDINATES: protein motif:HMM:NF008228.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarC family NAAT transporter"
                     /protein_id="HQR48997.1"
                     /translation="MNWFTTYLGYAVGALLAILPIVNPLGAVPLVISVGAHLPEKERL
                     RQIRRACIYTFSLMAGFLVAGSLIMSFFGISIPGMRIAGGMILAFLGFGMLFPGADAS
                     AAKVPQPGEIRHDIAFTPLAMPGLSGPGTFAVVMSLSSQASARQGWERIADFLGVATG
                     ILSVVVISWLVLRAAEKFNRLLGATGMVALTRLMGFLMICIGVQFIINGTAVVVADPS
                     MWTGLAEALRQQQAR"
     gene            5962..>6122
                     /locus_tag="PL152_06660"
     CDS             5962..>6122
                     /locus_tag="PL152_06660"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HQR48998.1"
                     /translation="MHVHERHFVNGHRLEPPFPDGLELALFGLGCFWGAERKFWMLPG
                     VYSTAVGYAA"
     assembly_gap    6123..6222
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<6223..6884)
                     /locus_tag="PL152_06665"
     CDS             complement(<6223..6884)
                     /locus_tag="PL152_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding SYLF domain-containing protein"
                     /protein_id="HQR48999.1"
                     /translation="MQRITALIALALVVLASPGTARADEKTDQRLEHSRQVFETFTGL
                     AEQGIPGWLLERAYGIVVVPKVFEIAVLVPGGRGGKGVMAVRNEDGSWSNPVFLRLYG
                     GSAGFQVGMQTTDLVLVLMSRKSVEGISGGKFTLGVDASVAAGPVGRTASANTDPMFK
                     AQILSYSRSEGVFLGVSIEGTVLAVDDGADETAYGVSGILASQILEGKVGTPPQAARD
                     FTA"
     gene            6998..7879
                     /locus_tag="PL152_06670"
     CDS             6998..7879
                     /locus_tag="PL152_06670"
                     /inference="COORDINATES: protein motif:HMM:NF025887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR49000.1"
                     /translation="MKTAARIAMLASFALVIGRVAAQDGDAPLATDAAFAAMSVERGS
                     QRAFEEYLAADAVVFRPGPVVAREWFAAHEQGSGRLEWTPVAAAQDCSGQWALTTGPW
                     KYTAPEGGGGSEGHYLSLWRREPDGGWRVVLDNGVDHGPAADSAPSLESAVAALWPAL
                     RAKTCRAGSGAARLHDVEAALNQAVQSDGLSLALAHVAARGALAYRDDAAPETFEVAA
                     MTDAVYARGTQAESHFVGADPDSDFGYSYGVISASGASGASPTARSAYVRVWRWDGRH
                     WSLVIDMLTPMDAGDSR"
     gene            complement(7894..>8111)
                     /locus_tag="PL152_06675"
     CDS             complement(7894..>8111)
                     /locus_tag="PL152_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49001.1"
                     /translation="DNPYAHATGMVRSLPHPARPDFRVLANPIKVGGERLPARIAPAL
                     GEHTEELLREVGYSDAQILELRKAGAA"
BASE COUNT         1164 a         2732 c         2830 g         1285 t
ORIGIN      
        1 cgtatgcggg tgtgccgctc acgcaccgcg agcacgcgca gtccgtgcgg ttcgttacgg
       61 cgcacgacca cgagtcgttc gccgaggccg actgggccgc gttgtccgcc ggtcggcaaa
      121 cgctgtgcct gtacatgggg attgcagagc tcgcatcgtt cgctgcacga ctcatggcac
      181 atggtcggcg ctcggagaca ccggtggccg tcgtcgagaa cggctcgcgc accgaccagc
      241 gcgtgacgct cgcgagtctc gggcagcttg gcgacctttc ggttcgcgcg gcgctgcagt
      301 ccccggccat ggtgatcgtg ggcgaggtgg cggcactggc cgggacgctt gcctggttcg
      361 gcgaggagcc gcgccgctgg gagtcgctgc gccgggtcgc gtgagtgacc gcgtcgatcg
      421 gccggcccga ggtcacatcg tccaggcgat ctccgcgaac acggcgcgag gttcgcccgg
      481 aaagtaacgg taggtcccga acgagtagtc ggcgcggtcg gcgtagcgca cgtcgagcgc
      541 gttggtgacg cgcaggccgg cacaccactg cggagcgaat cgccacaggc cgcgcaggtt
      601 cacgagttcg tagccgccgt agcgatgggc gttcgcggca tcgagccagt acggtccgac
      661 caccatccat tccgcctcgg cttcgacgga tgcggtcgga cgccactcga ggccgtagcg
      721 gccgatctcg cggggagcgg tgtcgatgtc gttgccggcc acgagggtct cgccgccgtc
      781 cacccggcgc gagaagtcgt agcgatggat cgcgtatgtg cccgcggcga ccacgtccag
      841 cgacggcagg gcgcgccagc gcaggtcgta ttcgatgcca cggtgcgtgg tgcgcccatt
      901 gtcgacgttg tagccgttcg attcgcgcag gatcaccccg ctgcgcttca tgtcgtagac
      961 ggccagtgcg agtgacacgc gcgaccaggc gcccttgagc cccagttcga cggaatcgag
     1021 ttcctccggc tgcaggtcgg cgactcgctg catgcgctgc agccgataca gctccgtcat
     1081 ctccggcggc cggaaaccct gtgtcagcgt cgcgtacccg accagggacg gcgtgaaatc
     1141 gtaggacacg ctgagcttcg gcgcccagtc gttgaaatgg tccgtgcggt cggcgggccg
     1201 gctgtacagg cacggcgccg gcgtgcacgg caccccctgg tcatcggtgt tgccgtcgag
     1261 catccggttg tcgtagtcgt acgtcaccca ttcggcgcgc acgccggccg tgattcgcca
     1321 gcgttcggca aagcggcgct ccgcctgcgc atacagcgac tcgacgttcg accgcacctc
     1381 gtagtcgtag tgcttgccgg ctggccggat ggcgttggcc gccgccgagc cgcccgtggt
     1441 ggggtggtcc tggtactcct cgagcgacga ctgcccgagt tcgacgtcgg ccccgacgat
     1501 gaccgaccag ccctgcacgt caccccaggc aatgctcgtc aggagcccgc cgctcacctg
     1561 gccgttcttc tccaccggtt ggcccagcag gaaatgctgc aggaaatcca tgtgcgaact
     1621 gcgcaggaat ggccgcaagt cgagttcggc gccatccagc aaacgaggct gcaggaggcc
     1681 cgtgaggcgc acggccgtcg aattgcgaaa tgcgcccggc gtcgggttcg attcgctgag
     1741 ctcgaggctc cggtacgcat tcttgccggt gatgaatccc gccgtctcct ggtcgaggtt
     1801 cgtgacggcc aggtctgcac gtgccggtgt cgtgcccagg tcaccgacga gcgtcgcatt
     1861 gaacttggcc tcgtcgtagc cggaatcgtc gcgccagccg ccgtcgtgcg tatacaagcc
     1921 cgcggcgccg aagtcggcgg cgctgccgtc gtgtcgaccc gccaccttga cgcgccagta
     1981 gctcgacggg ccggcgtcca cgctggcggc gaggggaggg cgatccagcg gtgccggctg
     2041 gatgacgttc accgtgccga acatcgcgtt cgagccgtag agggcgctgc cgggcccgcg
     2101 cagcacctcc accgcctgag cctgctccgt gttgacctcg tagagcgcgt tcacgttgca
     2161 gaagcccacg ggacgcaccg gcacgccgtt ctcgaggaac aggaacgcgc cgcaggaccc
     2221 ggcgcctgtc aggaccggcg agcggatggc cgtgagactc tcctggccgc tgccctgctg
     2281 gatcatcacg ccgggaatgc ggttaaggat ctcggcgtgg tgcgtcgcgt cgaccagcac
     2341 gatgccatcg gtgccgaccc ggccgatgct gagcggcacg tcgagcgcgg gctgcggctt
     2401 gcgcgtcgcg gtgacgacga cttcttcgag cggccccgca ggttccgcat ggagcgcggc
     2461 cgcgtgactc gcgtacagca cgcctgctgc gatgagcagg cgccgcagtc ggctggagtc
     2521 gtcacggcgc gggacgcagg cgtcggtcat cgcgggcccg gcaggaaagc tgcagcgatt
     2581 atccggcatc gatccggtgt ccgtacgacg gtttgttaac atcgatgcag ggatctccgg
     2641 agcattcaca tgcggatccg atgcgtgctg atgacggcct gcgcgatgct gctgatcgcc
     2701 gggcccgggc gggtcatggc ggacgacgcg ccgcaggagc cggccgcttc ccggccgaag
     2761 atcggcctgg tgctgagcgg cggcggcgcg cgtggcgcgg cccacatcgg cgtaatccgc
     2821 gccctggagc agatgcacgt cccgatcgac gccgtggcgg ggaccagcat gggcgccgtc
     2881 gtcggcggtc tctacgcggc cggcctcagc ggcgacgaga tcgagcaggt gttccgttcg
     2941 ctcgactggc aggagctgct ccgcgatcgc gcgccgcgcc gtgacctcgt ctatcgacgc
     3001 aagctcgacg accgcagctt cctcgcgacg ggcggtctcg gcatccgtgc cgacgagggc
     3061 gtcgtactgc cgctcggcct catcaccggc cagaagatca cgcaggtgct gcgcacctcc
     3121 acgatgcgcg tcgccgacgt gcaggacttc gacaagctgc cgaccccgtt ccgcgggctg
     3181 gcgacggacc tcgagaccgg cgacccggtg gtgctgcgct ccggcgacct cgcgaccgta
     3241 ctgcgcgcga gcatgtcggc gccgggcgtg ctcgccccgg tcgagatcgg tgatcgcctg
     3301 ctcgtcgacg gcggtctcgt cgacaacctc ccggtcgacc tcgcgcgcca gatgggcgtc
     3361 gacgtcgtca tcgccgtgga cgtgagcttt ccgctggcgc ggcgcagcgg cctcgagacg
     3421 ccactcgacg tcaccagcca gatgatcgcg atcatggtgc gtcggggcac cgcagcgtcg
     3481 ctcgcgaacc tgcgcccggg cgacgtgctg gtcacgcccg agctcgggcg catgacgtcc
     3541 atcgatttca cgaaaatgcc ccaggtcatc gtggcggggg agacggcggc cgaccagcag
     3601 tcggcgaagc ttgcgtccct ggcattgccc gcggaccagt atgcggccta cgtcgccgca
     3661 cgggcgcgcg cggccgatcc gaacgtgcgt gtcgcattcg tgaggccggg accgcattca
     3721 gaggccgatg cgaagcgcat cgacgcggtg ttcggcgacc tcgccggcaa gccgctcgac
     3781 acgcgcgagc tgcagcagcg ggcctactcc cagtacgcgc tcgaccgcta cgaagcggtg
     3841 gactaccgcg tcgtgaacga cgacggcgag cgcggcctcg agatcgacct gcgacgcaag
     3901 tcctgggggc cgaatttcct gcgcttcggc atgagcatcg agagcgacta cgagggtggc
     3961 acgtacgcga acgccggtgc acgcctgctc atgacgggcg tcaaccggct cgacggcgaa
     4021 ttgctgctcg acggtcagct cggcgaggac ccgcacttct acacggaact gttccagccg
     4081 ctgtcgttca agacggagtt cttcatcgcg ccggccttcc gctacgacct cgagacgctg
     4141 cagtacgtgc aggacgggca ccggctcgct cagtaccggg tgcgggacac cgagttcctg
     4201 ctcgcggtgg gggcggaact ctcgaactgg ggggaagtgc gcctcggcgt gcgccgtggg
     4261 accgggagca gccgactgat ggtgggcgag ccggtgttcc ccaacgagga attcgacctc
     4321 ggcgccgcgt acctcgaatt cggctacgac cgcctcgaca gcgcgtactt cccgaagcac
     4381 gggcaggcat tccgggcgag ctggcagtcg gagagcaagg cgctcggcgc cagcgccaac
     4441 gcggacatcg tcgaggcgtc gtgggtcttc gcccgcagcc acgatcgcaa cagcctggtg
     4501 ctgtccctcg cgggtggctc ggcgctcgac gaccgcgtcg tctcgccgca ggaattgttc
     4561 accctgggtg gcttcctcaa cctgtcgggg ctcccgcagg acgacctgat cggcacccag
     4621 tacggcatcg cgcgtgccat cgtctaccgc aggatcagcc ggggcggcac ggggctgttc
     4681 gagtttccgg cgtacatcgg cgcctcgctc gaagccggca acgtctggca gaccaaggcg
     4741 gacgtcgatt tcggcgacct caaggtcggg ggcagcggat tcctggccgc ggagactccg
     4801 ttcgggccgt tgtatctcgc cgcgggattc gcggagggcg acgccgcgct gtacctcgtc
     4861 ctcggcaaga ccttctgagc cggcgattcg cgctagaatc gccggccgat cccgcggtgt
     4921 agccaccatg acgacctgcc acgagcacaa cctgcgcgcc ccgctgccgg cagcccggcc
     4981 ctacgggatc cgggtccgcc tgcgccacga cgacccgttc cgcagcctgc tgggtgacga
     5041 ctggcaccgg gagcactggt tcgcgacccg cgcggaacgg gatcgagcgc tcgaggagat
     5101 gagcggccgc tacgtgtact tccgtccggg cgacgcgccg gcgctcgatt tcgagcgcgt
     5161 ggacccgcgg gccgcgtgaa ctggttcacg acgtacctcg gttacgccgt gggagcgctc
     5221 ctggcgatcc tgccgatcgt caacccgctc ggggcggttc cgctcgtgat ctcggttggc
     5281 gcacacctgc cggagaagga acgcctgcgc cagatacgcc gggcgtgcat ctacacgttc
     5341 tcgctcatgg ccgggttcct ggtcgccggc agcctcatca tgagcttctt cggcatctcg
     5401 atcccgggca tgcgcatcgc gggcggcatg atcctcgcat tcctcggctt cgggatgctg
     5461 tttcccggtg cggacgcatc cgcggcgaag gtgccgcagc cgggcgagat ccgccacgac
     5521 atcgctttca cgccgctggc gatgcccggc ctgagcgggc cgggcacctt cgccgtggtg
     5581 atgagcctct cgtcgcaggc cagcgcccgc cagggctggg agcgcatcgc cgatttcctc
     5641 ggagtggcga ccggcatcct ctcggtcgtg gtgatctcgt ggctcgtgct gcgcgcggcg
     5701 gagaagttca atcgcctgct cggagcgacc ggcatggtcg cgctcacgcg gctcatggga
     5761 ttcctgatga tctgcatcgg cgtgcagttc atcatcaacg gcaccgcggt cgtcgtcgcc
     5821 gacccgtcga tgtggacggg cctggccgag gcgctgcggc agcagcaggc gcggtaggga
     5881 gcgctcgcca tgggcctgtg gtcgacgcgc aagagccgga tgcccgcgcc tgcggacgcg
     5941 ctccccgggc gagacgtgcg gatgcacgtt cacgagcgac atttcgtcaa cggccatcgc
     6001 ctcgaaccgc cgttcccgga cggcctggaa ctcgcgctct tcggactcgg ctgcttctgg
     6061 ggtgcggagc ggaaattctg gatgctgccc ggcgtctaca gcacggcggt cggctatgcc
     6121 gcnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcggtgaa atcgcgcgcc
     6241 gcctggggcg gcgtgccgac cttgccctcg aggatctgcg aagccaggat gccggagact
     6301 ccatacgccg tctcgtcggc gccgtcgtcc acggcgagca ccgtgccctc gatggagacg
     6361 ccgaggaata caccctcgct gcgcgaatag gacaggatct gcgccttgaa catcgggtcg
     6421 gtgttcgccg acgccgtgcg gccgaccggg ccggcggcga cactcgcgtc gacgccgagc
     6481 gtgaacttgc cgccgctgat gccctcgacg ctcttgcgcg acatgagcac cagcaccagg
     6541 tcggtcgtct gcatgcccac ctgaaatccg gcactgccgc cgtacagccg caggaacacc
     6601 ggattgctcc agctaccgtc ctcgttgcgc acggccatca cgcccttgcc gccgcgcccg
     6661 ccgggaacca gcaccgcgat ttcgaacacc ttgggcacca cgacgatgcc gtacgcccgc
     6721 tccaggagcc agcccgggat gccctgttcg gcgagcccgg tgaaagtctc gaagacctga
     6781 cgcgagtgct cgagtcgctg gtccgtcttt tcgtccgccc gggcagtccc ggggctggcc
     6841 agcacgacga gggcgagggc gatcagtgcg gtgattctct gcatttgggc tctgaggggt
     6901 tggtgaggtg acgaccgact ttagcagaat acgggcgcgc gcggccgagg gcgcccggct
     6961 atgctcgtca tgcccgcact ggatcgaggc agcacccatg aagaccgcgg ctcggatcgc
     7021 aatgctcgcg tcattcgcct tggtgatcgg ccgagtggcg gcccaggacg gcgacgcgcc
     7081 actggcgacc gatgcggcgt tcgcagcgat gtccgtcgaa cgtggctcgc agcgggcgtt
     7141 cgaggagtac ctcgcagcgg acgccgtggt gttccgtccg ggcccggtgg tggcgcgtga
     7201 gtggttcgcg gcgcacgagc agggcagcgg caggctcgaa tggacaccgg tcgcggcagc
     7261 gcaggactgc agcggccagt gggcgctgac gaccggtccg tggaagtaca ctgccccgga
     7321 aggcggcggc ggctccgagg gtcattacct gtccctctgg cggcgcgaac cggacggcgg
     7381 ttggcgcgtc gtcctcgaca acggtgtgga tcacggcccg gcggcggaca gtgcgccctc
     7441 gctggagtcc gccgtggccg ccctctggcc ggccctgcgc gcgaagacat gccgggccgg
     7501 gagcggcgcg gccaggctgc acgacgtcga ggcggccctg aaccaggctg tgcagtcgga
     7561 cgggctgtcg ctggcgctcg cgcacgtcgc cgcccggggc gcgctggcct atcgggacga
     7621 tgcggcgccc gagaccttcg aggtggccgc catgaccgac gcggtctacg cgcgaggcac
     7681 ccaggccgag tcgcacttcg tgggggccga tccggactcc gacttcggct acagctacgg
     7741 tgtgatctcg gcgtcaggcg cttccggtgc gtcgccgact gcgcgctccg cgtacgtccg
     7801 cgtgtggcgc tgggacggcc gccactggag cctggtcatc gacatgctga cgccgatgga
     7861 cgccggcgat tcgcgttgaa gccggtcgcc ccgtcaggcg gcgcctgcct tgcgcagctc
     7921 gagaatctgg gcatcggagt aacccacttc ccgcaacagt tcttcggtgt gctcgccgag
     7981 cgctggcgcg atgcgggcag gcaggcgctc gccaccgacc ttgatcgggt ttgccagcac
     8041 gcggaagtcg ggtcgcgcgg gatgcgggag cgagcgcacc atgcccgtgg cgtgcgcgta
     8101 gggattgtcg a
//
LOCUS       DAOMFD010000091         7943 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_2961_length_7943_cov_34.834686, whole genome shotgun sequence.
ACCESSION   DAOMFD010000091 DAOMFD010000000
VERSION     DAOMFD010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7943)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7943)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7943
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2961_length_7943_cov_34.834686"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="PL152_06680"
     CDS             <1..840
                     /locus_tag="PL152_06680"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HQR49002.1"
                     /translation="AILNNLEHDHADIYPDVASIQRQFHLLLRTIPAAGRIVMNGADH
                     NLEQVLAMGCWTPVERFSSHASVAADWRLEADPASAYARYSVYRSGQRVGDVTWGLLG
                     RHNAENGLAALAAAHHAGVDVQAGIEALNRFQGVRRRLEVRGTVDGVVVYDDFAHHPT
                     AIASTLEGVRLKAGGGRVIAVLEPRSNTMKLGTHKTALAESLRGADRVFVYKSPEVKW
                     DVADAMRPLGSQAAVHEGLEPLVTALVAEARPGDHLVLMSNGSFGGLHEKLLAALKAR
                     PAA"
     gene            976..1341
                     /locus_tag="PL152_06685"
     CDS             976..1341
                     /locus_tag="PL152_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49003.1"
                     /translation="MDDTRAAEILKSLASGVDPADGTSAQAGGVLQSPDVVRALFIAA
                     ETLEGRVRMARRVSNLPRNAGKPWSREEDERLLAGFDGGVTVDALAGAHERTRAGIEA
                     RLVKHGRLEAEQAAGVRMR"
     gene            1490..2560
                     /locus_tag="PL152_06690"
     CDS             1490..2560
                     /locus_tag="PL152_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49004.1"
                     /translation="MTDRPRYLFVPVSGPGGAGELMRCLIIARGLRCVQPQADIHFIV
                     SRTAVFREAVEFPIHDSDASPTLSTPQVLMALEQFAPNVVVFDNSGRTSQLRAARRGG
                     ARVVFSSRTPHLRRKAFRLKWMRLLDEHWMVFPRFITGELGWSEKLKQRLFPHYRSRH
                     LDTLFPPSEPGARSAWLASRGLTEGQYVAFVPGGRGESSRVAQPTELFVAAARDYVSR
                     TGGPAVVLTGRTQGPDGGDTAGLTLLPRISPEEVQHALAGADFVVSNGGTTMIHALAH
                     GRPLVAVPLAADQGRRIRRAVKLEAVATAPSTPEGIAVAASSLHEDAARRSAMARRIG
                     ELGISNGLAEAVAALVSLAARR"
     gene            complement(2557..3585)
                     /locus_tag="PL152_06695"
     CDS             complement(2557..3585)
                     /locus_tag="PL152_06695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HQR49005.1"
                     /translation="MRDLAAPFRTLHGTLRDARATAFAVVRNESYYLRSFLDHHRRLG
                     IDQFIILDDQSTDGTRELLLLQPDCLVLESPFAFGERVVVPGSWPERRERAGILFKSL
                     IPQRHLAGRYSLYLDADEYLVLPAGVASVGDLFEVLARNRIPSVAAGLIDFYPESAGD
                     LDVPRHLLTSQQMLGAHPYFDAVPLLGWREGATSPHRTYDGATARLFRKHGIRHLPAW
                     ARGAPRWLRRMLPFRYPTPSVLKTPIVHWAPGVEYLNSHKANVPCTGQVVVGLAHMKF
                     THDLSRRVAYAIESEAYTRGSEKYLSYGALLASMRSGDPSFLGPDSRRYTAPADLAAA
                     GLTRLDLD"
     gene            complement(3648..4628)
                     /locus_tag="PL152_06700"
     CDS             complement(3648..4628)
                     /locus_tag="PL152_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49006.1"
                     /translation="MSKPTLYYHVGSHKTGTTAIQQTLLAAAPDLRRQGICWPVLGPS
                     RAHSRLVRAAYAWGLQGRVKANWLAARVRWQSRGMRATVLSSEKIYRIGYEFFEQAVQ
                     DTPGNRARRIAVLHRLRDLFSDDFDVRVLLYLRRLDEFAESMFKELLFRKPYAGRFLF
                     DDFLAEQAMLFDYAGQARELEQHLGPVSLLSYDAARRSGLIAHFCAQIGAASPRSADA
                     DPRVRRSASNTAALFLERLAGERAVSHADRLRLLDYTLAGGLPDPPGRMRSLWASRDA
                     LEAFMQRYRDPALDHLFPAIDWDRVQFGPLGDAEFQACRAAFDAWRPERV"
     gene            complement(4666..5940)
                     /gene="hemL"
                     /locus_tag="PL152_06705"
     CDS             complement(4666..5940)
                     /gene="hemL"
                     /locus_tag="PL152_06705"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0042286 - glutamate-1-semialdehyde
                     2,1-aminomutase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HQR49007.1"
                     /translation="MSLFTEACKVIPGGVNSPVRAFRGVGGEPIFFARAKGPHVWSAD
                     GRQFIDYVGSWGPMILGHADPQVIRVVQETAANGLSFGAPTAIETVVARKIIELVPSI
                     ELVRMVSSGTEATMSAIRLARGYTRRNRIVKFEGCYHGHSDSLLVKAGSGALTLGVPT
                     SPGVPPELAAYTTTLAYNDIAQLRAALAEFGGEVACVIVEPVAGNMNCIPPVPGFLEA
                     LREECTKHGAVLIFDEVMTGFRVARGGAQELYGIRPDLTTLGKIVGGGMPVGAFGGRR
                     DIMEHIAPLGPVYQAGTLSGNPVAMAAGLATLDGISAPGFHARLAAATDRLVTGLRHA
                     ATSAGVPLATNHVCGMFGLFFTSAERVTSYTDATACDVERFKRFFHGMLDEGVYLAPS
                     AFEAGFVSAAHTDHEIDATVAAAARVFSRIRA"
     gene            complement(6031..6906)
                     /locus_tag="PL152_06710"
     CDS             complement(6031..6906)
                     /locus_tag="PL152_06710"
                     /inference="COORDINATES: protein motif:HMM:NF014066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HQR49008.1"
                     /translation="MRTLAKLLLGALVLVGPATASAREQSILWAVQGRSNTVYLLGSI
                     HALKPDDTRLPAAAERAFDDSERLVMEIDMDDAASDPLAMAGQMQQAALLPQGQTLRS
                     VLGTDYSSINERTRKVGLDLALLDGFAPWFVAINVMDLQLAQHGLSSDYGVEQTLAAR
                     AVARHKPIEGFETAAQQFGMLAAMPLPMQKRFLQMTLEDSANFDHEVDDLVRAWKTGD
                     TTALARMLADESREFPDLYRRLTVDRNRAWALRIEDLLDDHDDYLVVVGAMHLVGPDS
                     VVDLLRKRGHLITQQ"
     gene            6995..7657
                     /locus_tag="PL152_06715"
     CDS             6995..7657
                     /locus_tag="PL152_06715"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR49009.1"
                     /translation="MSNRTIAVTDSLYDYLLAVSLRETDLQRRLREETAANPSARMQI
                     APEQGQFMALVARMIGAKRCLEVGVFTGYSSLAVALALPDDGRIVACDVSEEWTAVAR
                     RYWQAAGVAHKVELRLAPALETLDGLIMAGEAGRYDLAFIDADKTNYLAYYERVLTLL
                     RPGGVVMTDNTLWSGRVADPEVGDADTVALRHYNEHLHRDERVDLSLVPIGDGLTLAR
                     KK"
     gene            complement(7734..>7943)
                     /locus_tag="PL152_06720"
     CDS             complement(7734..>7943)
                     /locus_tag="PL152_06720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HQR49010.1"
                     /translation="NQVLAQIELYRHGAKYGKQVYVLPKHLDEKVAKLHLERIGARLT
                     TLTQEQADYIGVPVQGPYKAEHYKY"
BASE COUNT         1218 a         2673 c         2849 g         1203 t
ORIGIN      
        1 gcgatcctga acaacctcga gcacgaccac gccgacatct acccggacgt ggcctccata
       61 cagcggcagt ttcatctcct gctgcgtacg atcccggcgg ctgggcgcat cgtcatgaac
      121 ggtgcggacc acaacctcga gcaggtcctc gcgatgggct gctggacgcc ggtcgagcgc
      181 ttttcgtccc acgcgtcggt tgccgccgac tggcggctgg aggcggaccc ggcatcggcc
      241 tatgcgcgct actcggtgta ccggtcgggg cagcgggtcg gcgacgtcac gtggggcttg
      301 ctcgggcggc acaacgcgga aaacggcctt gcggcgctgg ccgcggcgca ccacgcgggc
      361 gtcgacgtgc aggcgggcat cgaggccctc aatcgattcc agggcgtgcg ccggcgactc
      421 gaggtgcgcg gcaccgtgga cggcgtcgtc gtctacgacg atttcgcgca ccatccgacc
      481 gcgatcgcga gcacgctcga gggcgttcga ctcaaggcgg gcggcgggcg cgtcatcgcg
      541 gtgctcgagc cgcgctccaa caccatgaag ctcggcacgc acaagacggc cctcgcagag
      601 tcgctgcgcg gcgcggaccg cgtgttcgtg tacaagtcgc cggaagtgaa gtgggacgtc
      661 gccgatgcga tgcggccgct cgggtcccag gccgcggtgc acgagggact cgagccgctg
      721 gtcacggcgc tggtcgccga ggcccgcccc ggcgaccacc tggtgctgat gagcaacggc
      781 tcgttcggtg ggctgcacga gaagctgctc gcggcactca aggctcgccc ggccgcttga
      841 gccggccgcg acgccgacac cgtccatgtg attctccacg actgcggcga ttttgccgct
      901 ggcatcgcgc gtcgccgcgt acttgaatgg cgctgacggc tgggtcggct ggccgcacac
      961 cggaggtcgt ggaccatgga cgatacgaga gccgcagaga tcctgaagtc actggcgtcg
     1021 ggcgtggatc cagcggatgg aaccagtgcc caggcgggtg gcgtgctgca gtcgccggac
     1081 gtcgtgcgcg cgctgttcat cgcagccgag acgctcgagg gtcgcgtgcg catggcgcgc
     1141 cgcgtgtcga acctgccgcg caacgcgggc aagccgtggt cgagggagga ggacgagcga
     1201 ctgctcgccg ggttcgacgg cggggtcacg gtggatgcgc tggccggcgc ccacgaacgc
     1261 acccgggcgg gcatcgaggc gcgactggtc aagcacggcc gcctcgaggc agagcaggcc
     1321 gccggggtcc gcatgcgctg agcattgccg ccacgctcac gcgctagaat cccgcccgtt
     1381 gccggcagcg ggcgggacac atgcgcgaca ggcgcggcgg gatgcaaacg gtgatcgggt
     1441 cgacctgctg cggtgggatc gcgcgcggcg cgggtgcggg cggcggcaca tgaccgaccg
     1501 accgaggtac ctgttcgtcc cggtgtccgg cccgggcggc gccggcgaac tgatgcgatg
     1561 cctcatcatc gcgcgcggac tgcggtgcgt gcagccgcag gccgacatcc acttcatcgt
     1621 gagccgtacc gccgtgtttc gcgaggcggt cgagttcccg atccacgaca gcgatgcgtc
     1681 gccgacgctc agcaccccgc aggtgctcat ggcgctggag caattcgcgc cgaatgtcgt
     1741 cgtattcgac aactcgggcc gcacctcgca gctgcgtgcc gcgcggcgcg ggggcgcgcg
     1801 tgtggtcttc tcgagccgca cgccgcacct gcgtcgcaag gccttccgcc tcaagtggat
     1861 gcgactgctc gacgagcact ggatggtgtt cccgcgcttc atcacgggcg aactcggctg
     1921 gtccgagaaa ctgaagcagc gactgtttcc gcactatcgc agccgccatc tcgacacgct
     1981 ctttcccccg tcggagcccg gcgcacgctc ggcgtggctg gcgtcccgcg gcctcaccga
     2041 agggcagtac gtggcattcg tgccgggcgg gcggggcgag tcgtcgcgag tggcgcagcc
     2101 caccgaactg ttcgtggccg cggcgcgcga ctacgtgtca cgcacgggtg gccccgccgt
     2161 cgtgctgacc gggcggacgc aagggccgga cggaggtgac accgctgggc tcacgctgct
     2221 gccgcggatt tcgcccgagg aagtgcagca cgcgctggcc ggcgcggact tcgtcgtgag
     2281 caatggcggg acgaccatga tccatgcgct ggcgcacggc cggccgctgg tggcggtgcc
     2341 gctcgcggcg gaccagggcc ggcgcatccg gcgcgcggtg aaactggagg ccgtggcgac
     2401 cgcgccgtcc acgcccgagg ggattgcggt cgcggcatcg tcgttgcatg aggatgccgc
     2461 gcgccgcagc gccatggcgc gccgcatagg cgaactgggc atcagcaacg gtctcgcgga
     2521 agccgtcgcg gcgctggtgt cgctcgcggc gcggcgctag tcgaggtcga gacgcgtgag
     2581 ccccgcggcc gcaaggtcgg cgggcgccgt atatcggcga ctgtccggac cgaggaagct
     2641 cgggtcgccg ctgcgcatgc tcgcgagcag cgcgccgtag gaaaggtact tctcgctccc
     2701 gcgggtgtag gcctcggact cgatcgcgta ggcgacgcgc cgcgacaggt cgtgcgtgaa
     2761 cttcatgtgc gcgaggccga ccaccacctg gccggtgcac ggcacgttcg ccttgtgcga
     2821 gttcaggtat tcgacgcccg gcgcccagtg cacgatcgga gtcttcaaga ccgaaggcgt
     2881 cggataacgg aagggcagca tccggcgcag ccagcgcggg gcgccacgcg cccacgcagg
     2941 caggtgccgg atgccatgct tgcggaacag tcgcgcggtc gctccgtcgt aggtgcggtg
     3001 cggactggtc gcgccctcgc gccagccgag cagcggcacc gcgtcgaaat acgggtgcgc
     3061 gcccagcatc tgctgcgacg tgagcaggtg gcgcggcacg tccaagtcgc cggcgctctc
     3121 cgggtagaaa tcgatcaggc cggccgcgac gctcggaatc ctgttgcgcg cgaggacctc
     3181 gaacaggtcg ccgaccgacg cgacgcccgc gggcaggacc aggtactcgt ccgcatccag
     3241 gtagagcgag tagcggcccg ccaggtgccg ttgcgggatg agcgacttga acaggatccc
     3301 tgcgcgctcg cgccgctccg gccagctgcc cggcaccacg acgcgttcac cgaacgcgaa
     3361 gggcgactcg aggaccaggc agtccggctg caggagcagc aactcgcgcg tgccgtcggt
     3421 cgactggtcg tcgaggatga tgaactggtc gatgccgagc cggcggtgat ggtcgaggaa
     3481 cgagcgcagg tagtagcttt cgttcctgac gaccgcgaac gccgtcgccc gtgcatcgcg
     3541 cagcgtgccg tgcagggtgc gaaatggggc cgcgaggtca cgcatcgacg tacaccttca
     3601 ttcgcctgct cgccgcgccg tccaacgggg aggagatcat gcgcgcctca cacgcgttcc
     3661 ggtcgccacg cgtcgaatgc ggcacggcac gcctggaact ccgcgtcgcc caacgggccg
     3721 aattgcaccc gatcccagtc gatcgccggg aacaggtggt cgagcgcggg gtcgcggtat
     3781 cgctgcatga acgcctccag cgcgtcgcga gatgcccaca gcgagcgcat ccgcccgggc
     3841 gggtcgggaa gcccgccggc cagggtgtag tccagcaggc gcaggcggtc ggcgtgggac
     3901 acggcacgct ccccggccag tcgctcgagg aacagcgctg cagtattcga tgcgctgcga
     3961 cggacgcgcg ggtcggcatc ggccgatcgc ggtgaggccg cgccgatctg cgcgcagaag
     4021 tgcgcgatca atcccgaccg gcgggcggcg tcgtacgaca gcaggctcac cggaccgaga
     4081 tgctgctcga gctcgcgcgc ctgtccggcg taatcgaaca gcattgcctg ctcggcgagg
     4141 aaatcgtcga acaggaacct gcccgcgtac ggcttcctga acagcagctc cttgaacatc
     4201 gactcggcga actcgtcgag gcgccgcagg tacagcagca cgcggacgtc gaaatcgtcg
     4261 ctgaacaggt cgcgcaggcg gtgcagcacc gcgatgcgtc ttgcgcggtt cccgggagtg
     4321 tcctggacgg cctgttcgaa gaactcgtag ccgatgcgat agatcttctc cgagctgagg
     4381 accgtcgcac gcatgccgcg cgactgccag cggacacggg cggccagcca gttcgccttg
     4441 acgcgtccct gcaggcccca cgcgtacgcg gcgcgcacca gcctcgagtg cgcgcggctc
     4501 ggaccgagca ccggccagca aatgccctgc cggcgcaggt ccggtgcggc ggccagcagc
     4561 gtctgctgga ttgcggtcgt gccggtcttg tgcgagccga cgtggtagta gagcgttggc
     4621 ttgctcatcg ggctgcttcc tgcggccggg gccgctagcg acccgtcatg cgcggatcct
     4681 ggagaaaacg cgcgctgctg ccgcgaccgt cgcgtcgatc tcgtgatccg tgtgggccgc
     4741 ggacacgaat cccgcctcga aggccgacgg cgcgaggtac acgccttcgt cgagcatgcc
     4801 gtggaagaag cgcttgaagc gctcgacgtc gcaggcggtc gcgtcggtgt acgaggtgac
     4861 ccgctcggcg ctggtgaaga acaggccgaa cattccgcac acgtgattgg tcgcgagcgg
     4921 cacgccggcc gatgttgcgg cgtgccgcag cccggtcacg agccggtccg tcgccgccgc
     4981 gagccgcgcg tggaaccccg gggcggaaat gccgtcgagc gtcgcgagtc cggctgccat
     5041 ggcgaccggg ttccccgaca gcgtgcccgc ctggtagacg gggccgagcg gcgcgatgtg
     5101 ctccatgatg tcgcggcgac cgccgaatgc gccgaccggc atgccgccgc ccacgatctt
     5161 gccgagggtc gtgaggtcgg ggcggatccc gtagagttcc tgcgcgccgc cacgcgcgac
     5221 gcggaagccc gtcatcactt cgtcgaagat cagcactgca ccgtgcttcg tgcactcctc
     5281 gcgcagggcc tcgaggaatc cgggcacggg cgggatgcag ttcatgttgc ccgcgaccgg
     5341 ctcgacgatg acgcaggcca cttcgccgcc gaattccgca agggccgcgc gcagctgcgc
     5401 gatgtcgttg taagcgaggg tcgtcgtgta tgccgcaagc tcgggcggaa cgccgggcga
     5461 ggtcggcacg cccagggtca gcgcgcccga gcccgccttc accagcagcg agtccgagtg
     5521 gccgtggtag cagccctcga acttcacgat gcggttgcga cgcgtgtagc cgcgcgcgag
     5581 ccggatcgcg ctcatcgtgg cctcggtgcc cgagctcacc atgcgcacga gctcgatcga
     5641 cggcacgagc tcgatgatct tccgcgcgac caccgtctcg atcgcggtcg gcgcgccgaa
     5701 cgacagtccg ttcgccgccg tctcctgcac gacccggatc acctgcgggt cggcgtgacc
     5761 gaggatcatc gggccccagg agccgacgta gtcgatgaat tgccggccgt ccgccgacca
     5821 cacgtgcggg cctttcgcgc gcgcgaagaa gatcggctcg ccgccgacgc cccggaatgc
     5881 gcgtaccggc gagttgaccc cgccgggaat gaccttgcag gcttcggtga aaaggctcac
     5941 gggcggctcc ggtcgctggg cacgatccgc atagtctagc aagcgctggc gatggggccg
     6001 gcgcgcggtg ccggtccggt ggcgccgccg tcactgctgg gtgatcaggt ggccgcgctt
     6061 gcgcagcagg tccacgacgc tgtcgggccc gacgaggtgc atggcgccga ccaccacgag
     6121 gtagtcgtcg tggtcgtcga gcaggtcctc gatccgcaac gcccaggcgc ggttgcggtc
     6181 cacggtgagg cgccggtaca ggtccgggaa ctcccgcgat tcgtcggcca gcatccgggc
     6241 gagcgccgtc gtgtcgcccg tcttccacgc gcgcacgagg tcgtccacct cgtggtcgaa
     6301 attggcggaa tcctcgagcg tcatctggag gaagcgcttc tgcatgggca gaggcatggc
     6361 ggccagcatg ccgaactgct gtgcggcggt ctcgaacccc tcgatgggct tgtggcgcgc
     6421 gacggcccgg gcggcgaggg tctgctcgac gccgtagtcg gacgagaggc catgctgcgc
     6481 gagctggaga tccatcacgt tgatcgcgac gaaccagggc gcaaagccgt cgaggagcgc
     6541 gaggtcgagc cccaccttgc gcgtgcgctc gttgatcgag gagtagtccg tgccgagcac
     6601 gctcctgagc gtctggccct gcggcagcag cgcggcctgc tgcatctggc ccgccatggc
     6661 gagcggatcc gaggccgcgt cgtccatgtc gatttccatg acgaggcgct cggaatcgtc
     6721 gaaggcccgc tcggcggcgg ccggcagtcg agtgtcgtcc ggcttcagcg cgtggatgga
     6781 accgagcagg tagacggtgt tcgaccggcc ctgcaccgcc cagaggatgg actgttcgcg
     6841 ggcggacgcc gtggccggac cgaccagaac gagagcgccc agcagcagtt tcgcgagagt
     6901 cctcatcggt cgctgcgtct cggtgaaacg ggcgtgcatg ccgtcatgtt gccactgtcg
     6961 cagtatgctg tggcgccgac gcgaggaaag gctcatgtcc aatcgcacga tcgccgtcac
     7021 ggattcgctg tatgactacc tgctggccgt atcgctgcgg gagaccgacc tgcagcgacg
     7081 gttgcgcgaa gaaaccgcgg ccaacccgtc ggcacggatg cagatcgccc ctgagcaggg
     7141 ccagttcatg gcgctggtcg cgcgcatgat cggggcgaag cgctgcctcg aggtgggcgt
     7201 cttcaccggc tactcgtcgc tcgccgtcgc acttgcgctg ccggacgacg gtcgcatcgt
     7261 ggcctgtgac gtcagcgagg aatggaccgc ggtcgcgcgt cgctactggc aggccgcggg
     7321 tgtcgcgcac aaggtcgaac tgcggcttgc accggcgctc gagaccctgg acgggctgat
     7381 catggcgggc gaggcggggc gctacgatct cgcattcatc gacgccgaca agacgaatta
     7441 tctcgcctac tacgagcggg tgttgacgct gctgcgcccg ggcggcgtgg tgatgaccga
     7501 caacacgctg tggagcggtc gcgttgccga ccccgaggtc ggcgacgccg acaccgtcgc
     7561 cctccggcac tacaacgagc acctgcaccg cgacgagcgc gtggacctga gcctcgtgcc
     7621 gatcggcgac ggcctcacgc tcgcccgcaa gaaatagaga aataggggac attctccatt
     7681 tttcgcgaga ggcggccgcc tttcccgaaa aatggagaat gtcccctatt tcttcagtac
     7741 ttgtagtgct ccgccttgta cggcccctgc accggcacgc cgatgtagtc ggcctgctct
     7801 tgcgtcaggg tcgtgagccg cgcaccgatg cgctcgaggt gcagcttcgc caccttctcg
     7861 tcgaggtgct tcggcagcac gtagacctgc ttgccgtact tcgcgccatg ccggtagagc
     7921 tcgatctgcg ccagcacctg gtt
//
LOCUS       DAOMFD010000092         7661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3169_length_7661_cov_30.909282, whole genome shotgun sequence.
ACCESSION   DAOMFD010000092 DAOMFD010000000
VERSION     DAOMFD010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3169_length_7661_cov_30.909282"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="PL152_06725"
     CDS             <1..440
                     /locus_tag="PL152_06725"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HQR49011.1"
                     /translation="RRMTAKKQFVALSEFRYQLARFQRFSTAASRDGGLTQTQYLLLL
                     HIAGHPERDWATVGELAHRLQGSPHGTAALVDRCVALRLVEKHRSTEDARCVEVHLTE
                     HGWRAVSRIARRHRDELQSLRQVFHVADVNDAASADRATSARR"
     gene            542..895
                     /locus_tag="PL152_06730"
     CDS             542..895
                     /locus_tag="PL152_06730"
                     /inference="COORDINATES: protein motif:HMM:NF022722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3079 domain-containing protein"
                     /protein_id="HQR49012.1"
                     /translation="MVVREQLERLHFDVEVDVLARSGPRATERMTKVEADIFHPTLVN
                     LRETLAVVAASQDPAAWQRSLGAALEGLRQAVRCLRRRSGNGTIRTPHPVELSGPEWI
                     EHGLDAETQRERGAE"
     gene            930..3173
                     /locus_tag="PL152_06735"
     CDS             930..3173
                     /locus_tag="PL152_06735"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric-oxide reductase large subunit"
                     /protein_id="HQR49013.1"
                     /translation="MDNVEQLSPRWRYGVAVTFVFGFAFLILLTFKAYHDAPPIPARV
                     IDPSGVVVFTGDDIRSGQEVFLEHGLMDNGTIWGHGGYLGPDFGAAVLHEWALALARQ
                     RAEARYGRDYAVLSGEERAAVDAEVASLLKTNRYDAERDTLTLLPAGAQVFQAEIGRW
                     EDYFRDPRRNGGLPANLVRDPQQLRELTAFFTWAAWASVADRPGTNHSYTNNFPYDPL
                     AGNRPTPAAVLWTAVSFIFLLGGTAIVLLAFGKFDYLGWHGNPVTPRPWVVSDSQQAT
                     LKYMAVAAILLLGQTLFGAGVAHYRADPGTFYGIDLSRWLPSNLMRTWHLQSAIFWIA
                     TAYVAGALFVAELLGRGSPRGQRLGIHVLFGAVALVVFGSMLGEWAGLLQWLPRSWFW
                     FGNQGWEYLEIGRFWQGLLAVGLLFWFFLVWRAVAPARRDPERSGFANFFLIAAFAIP
                     LFYLPALVFGSKTNYTIVDAWRFWIIHLWVEGFFEFFVTVIVAVIFHEMGLVRRLTAL
                     RTIYLDAILYFGGGLIGTGHHWYWTGQTELNMALSAAFSALEVVPLTLITLDAWEFVR
                     VTRGTASVVDRHRWTFYFLMVVGFWNFVGAGVFGFLINTPIVSYYEVGTILTPNHGHA
                     AMMGVFGMLGAGLIVFVLRETVSDQAWPRLSRLIRIAFWGLNAGLAMMVLFSLLPGGI
                     LQVYDVIENGYWHARSLAYTATPLARTLEWLRMPGDLVFMFAGAVPLALATTWGYLAL
                     LKRNRGT"
     gene            3324..5495
                     /gene="glgP"
                     /locus_tag="PL152_06740"
     CDS             3324..5495
                     /gene="glgP"
                     /locus_tag="PL152_06740"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HQR49014.1"
                     /translation="MLEKNEGIRSGAKDVRRAAQALAREIPARLAPLARVAYNYHWVW
                     QPDGEQLFRAIDSYRWRLCRLNPVRFLDEAPEESLERAASDLSLVRRAEALRNSLEED
                     LARPALENGVASMERPIAFFCAEFGLHRSLPIYSGGLGVLAGDVLKEASDRGLPMVGV
                     GLMYRQGFFHQRVDAGGWQHEYWTETDPERRACVLVTREDGSPLTVRVPIWDEEVSVN
                     VWRVEVGRVPLFLLDAEVSGNSPRQRFVSARLYEGNRQIRLAQYALIGIGGMRVLDAL
                     GIDPSVIHMNEGHPVTATMELLGREKERGASFAEACEMVRRKVVFTTHTPVPAGNETY
                     ALDEMMTVFPGAARRLGGDMEKFLSLGRFQPTDRNEPINMTALAIRMSRSTNGVSKIH
                     AGVARRMWQGLFPGRSAEEVPITQVTNGVHVPSWISPMMRRLLDQYLVPGWHTHERIT
                     DPATWAAVDRIPDAELWAVRQEYSRRLARWVRSRTVTDRLTRGDVMDYALKAANSFDG
                     EALTLGFARRLATYKRLHLLFQDPGRMLSLLDAPRPIQVLIAGKAHPRDDDAKRVLSR
                     MFNLKSDPRIGGRVAFLEDYHVGLASILTSGCGVWINLPRPPLEASGTSGIKAAFNGV
                     LNLSVLDGWWAEAYDGSNGWAIDGGEDADTAAKDARDAAALFDLLEREVIPMYYDRDA
                     SGVPHAWVARIKASLRTIGPRFCATRMLDEYARGIYAAAQP"
     gene            5558..6571
                     /locus_tag="PL152_06745"
     CDS             5558..6571
                     /locus_tag="PL152_06745"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HQR49015.1"
                     /translation="MGTLQRYARRAVTIPAVALALLALLAALPVLLPLAFVVDLARAA
                     GRRSFVSVRIVLFGVCFLATEVLGLALLFGVWLSSVGRPELRAARTWPVQRWYTAMHY
                     AALRTLFRLRFEVEGDAAATPGPLLVLVRHASIIDSLVPAVFIANVHRTRLRYVLKRE
                     LLVDPCLDLAGHWLPNRFVARGGVDTAREVDSVRSLKAGLEADEGVLLYPEGTRFTEE
                     KRRRVIERLGGAARERAERLQHLLPIRPGGVLALLDAPPACDVLFIAHHGLEGFAHVA
                     DVLRGALVGRTVRLRFWRVPAASIPAGAEKRRAWLADEWQRMDDWLSAEEDRDRTGLP
                     AVK"
     gene            6576..>7661
                     /locus_tag="PL152_06750"
     CDS             6576..>7661
                     /locus_tag="PL152_06750"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR49016.1"
                     /translation="MSDTIRLDETITVDRPLHEVFAYISEFSRIEEWDPAVARGQRLT
                     EGPLGVGSQFRIDMKAGFSLRYTVIEWERDRRLLMTVDSKPFTAREEIFFARTAAGTQ
                     VRYVATFEFVAPLARILNTFPGLMDRVGKSAMAGMRRALEDRFEPPGEDAWTALADRL
                     LVPGLVGFTRFGYRAARKHWNPVSAYLRDRHVVITGATSGLGLATATTLAARGAELTL
                     VARDRTKANGVARDLRRRFGNPRVHVEIADLSLLEDVRALVARLLAKGRAIDVLVNNA
                     GALINPREETAEGLEKSFALLLAGPYVLTEGLRPLLKQAAAGRGVARVVNVLSGGMYA
                     QKIHVEDLQSEQGKYSGAIAYARAKRGL"
BASE COUNT         1144 a         2550 c         2665 g         1302 t
ORIGIN      
        1 ttcgtcgcat gacagccaag aagcaattcg tcgcactgtc ggagtttcgc taccagctcg
       61 cgcggttcca gcgattcagc acggctgcct cgcgcgacgg cgggctgacg cagacgcagt
      121 acctgttgtt gctgcacatc gccgggcatc ccgagcgcga ttgggccacc gtgggcgagc
      181 tcgcgcatcg actgcagggg agtccgcacg ggacggccgc cctggtcgac cgctgcgtcg
      241 cattgcggtt ggtcgagaag catcgcagca ccgaggatgc ccgatgcgtc gaggtgcacc
      301 tgaccgagca cggctggcgc gcggtgagcc ggattgcacg acgccaccgc gacgaattgc
      361 agtcgctgcg gcaggtgttc cacgtggcgg acgtcaacga tgcagcgtcg gctgaccgtg
      421 cgacgagtgc gcggcgatga attacctcgg ccgcagtcgc acctcatgcg ccgccgggct
      481 gcgtgactat caccgtgccg tgcggcgcct tcgacagtgt gccaggcggg gaggcgaggc
      541 cttggtcgtc cgggagcagc tcgagcggtt gcacttcgac gtggaggtcg acgtcctggc
      601 acggagcggt ccgcgtgcga ccgagcgtat gaccaaggtc gaggccgaca tcttccatcc
      661 gacgctggtg aatctgcgcg agacgctcgc agtggtggcg gccagtcagg atccggccgc
      721 gtggcagcgg tcgctgggcg cggccctcga gggcctgcgg caagccgtgc gctgcctgcg
      781 gcgtcgatca ggcaacggca cgatccgcac gccgcacccg gtcgagctgt ccgggcccga
      841 gtggatcgag catggcctcg atgccgagac gcagcgtgag cgcggggccg agtagcgttc
      901 cgaacgggca cacccggttg ggagatcgca tggacaatgt cgaacagttg tccccacgct
      961 ggaggtacgg ggtcgccgtc accttcgtat tcggcttcgc cttcctcatc ctgctgacct
     1021 tcaaggcgta ccacgacgcg ccacccatcc cggcccgggt catcgacccg tccggtgtcg
     1081 tcgtgttcac cggcgacgat atccgcagcg gacaggaggt gttcctcgag cacgggctga
     1141 tggataacgg cacgatctgg ggacacggcg gctatcttgg gcccgacttc ggcgcggccg
     1201 tcctgcacga gtgggcgctc gcgctcgcgc ggcaacgcgc cgaggctcgc tacggcaggg
     1261 actacgcggt gctgtcgggc gaggagcgcg ccgccgtcga tgccgaagtg gccagcctgc
     1321 tgaagaccaa tcgctatgac gcagagcgcg acacactgac cctgctgccg gccggcgcgc
     1381 aggtgttcca ggcggagatc ggccgctggg aagactattt ccgcgatccc aggcgcaatg
     1441 gcggcttgcc ggcgaacctg gtgagggacc cgcagcagct gcgcgaactc accgcgttct
     1501 tcacctgggc ggcgtgggcc tcggtggccg atcgacccgg cacgaaccac tcgtacacga
     1561 acaacttccc ctacgatccc ctggcgggca atcgcccgac gcccgcggct gtgttgtgga
     1621 cggccgtcag cttcatcttc ctgctcggcg gcacggccat cgtgctgctc gcattcggca
     1681 agttcgatta cctgggctgg cacggcaacc cggtcacccc ccgcccgtgg gtcgtgagcg
     1741 actcgcagca ggcgacgctc aagtacatgg ccgtggccgc gatactgttg ctcgggcaaa
     1801 cgctcttcgg cgccggtgtc gcccactatc gcgcggatcc gggcacgttc tacggcatcg
     1861 acctgtcccg gtggctgccg agcaacctga tgcgcacgtg gcacttgcag tcggcgatct
     1921 tctggatcgc cacggcgtac gtggccggcg cgctgttcgt cgccgaactg ctgggccgcg
     1981 gcagcccgcg cggccagcgg ctcggcatcc acgttctctt cggtgccgtc gccctcgtcg
     2041 tcttcggcag catgctcggt gaatgggccg ggttgctgca gtggctgccg cgcagctggt
     2101 tctggttcgg caaccagggt tgggaatacc tggagatcgg acggttctgg cagggcctgc
     2161 tcgcggtcgg cctgctgttc tggttcttcc tggtctggcg ggcggtggcg cccgcacgtc
     2221 gcgatccgga acgcagcggc tttgcgaact tcttcctcat cgcggccttc gcgatcccgc
     2281 tgttctacct gccggccctg gtcttcgggt cgaagaccaa ctacacgatc gtcgatgcct
     2341 ggcgattctg gatcatccac ctgtgggtcg aggggttctt cgagttcttc gtcaccgtca
     2401 tcgtggccgt catattccac gagatgggcc tcgtccggcg gctcacggcg ctgcgcacga
     2461 tctatctcga cgccatcctg tatttcggcg gcggcctgat cggcaccggt caccactggt
     2521 actggaccgg acagaccgag ttgaacatgg cactgtcggc ggcattctcc gcactggagg
     2581 tcgtaccgct gacgctcatc acgctcgacg cctgggagtt cgtgcgggtg acgcgcggga
     2641 cggccagcgt ggtcgaccgg caccgctgga ccttctactt cctgatggtc gtcggcttct
     2701 ggaatttcgt cggggcaggc gtgttcggtt tcctgatcaa cacgccgatc gtcagctatt
     2761 acgaagtggg cacgatcctg acgcccaatc atggccatgc ggcgatgatg ggggtgttcg
     2821 gcatgctcgg cgcgggattg atcgtgttcg tgctgcggga gaccgtttcc gaccaggcgt
     2881 ggcccaggct gtcgcgcctg attcgcatcg ccttctgggg cctgaacgcc ggtctcgcca
     2941 tgatggtgct cttcagcctc ctgccgggag gcatcctgca ggtctacgac gtgatcgaga
     3001 acggctactg gcatgcccgc agcctcgcgt acacggccac gccactcgcg cgcacgctgg
     3061 aatggttgcg catgccgggt gacctcgtat tcatgttcgc gggcgccgtg ccgctggcat
     3121 tggcgacgac gtggggttac ctggcactct tgaagcgaaa ccgcggcact taggttgatc
     3181 cttgcgactg ctttcgcaaa ggccggcgac ccgctgaccc ggtcatgcgg ctcgcgaaag
     3241 aggttcgccg ccaactcccg gcgcgtcgga ttctgtgctt gaatcgccgc gcacgcgacc
     3301 gatcccgcgg gagtagccgg cccatgctcg aaaagaacga gggtatccga agcggcgcca
     3361 aggacgtccg tcgggcagcg caagcactcg ctcgagagat tccggcgcgg ctcgccccgc
     3421 tggcgcgggt cgcctacaac taccactggg tgtggcagcc ggacggagag cagttgtttc
     3481 gcgccatcga cagctaccgc tggcggttgt gccgcctgaa cccggtgcgc ttcctcgacg
     3541 aggcccccga ggaatcgctg gagagggcgg cctccgacct gtcgctggtc aggcgggcgg
     3601 aggcgctgcg gaactcgctg gaggaagacc tggcgcgacc cgcgctcgaa aacggcgtgg
     3661 cttccatgga gcggccgatc gcgttcttct gcgccgagtt cggcctgcat cgttccctgc
     3721 cgatctattc gggcgggctg ggcgtgctgg cgggcgatgt gctcaaggag gcgtcggatc
     3781 ggggcctgcc tatggtcggc gtgggactga tgtaccggca gggctttttc caccagcgcg
     3841 tcgacgcggg cggctggcag cacgagtact ggaccgagac cgaccccgag cggcgtgcat
     3901 gcgtcctggt cacgcgcgag gatggcagcc cgctcacggt gcgggtgccg atctgggacg
     3961 aggaagtgtc ggtgaacgtg tggcgggtcg aggtcgggag agtgccgctg ttcctgctcg
     4021 atgccgaagt gtcggggaac agcccgcggc agcgcttcgt gagcgcccgg ctctacgaag
     4081 gcaatcgcca gattcgcctg gcccagtatg ccctgatcgg aatcggcggg atgcgcgtcc
     4141 tcgatgccct cgggatcgac ccatccgtca tccacatgaa cgaagggcat ccggtcaccg
     4201 ccaccatgga actgctcggc cgggagaagg agcgcggcgc gagcttcgcc gaagcctgcg
     4261 agatggtgcg tcggaaagtc gtgttcacga cgcacacccc ggtgccggcg ggcaacgaaa
     4321 cctacgcact cgacgagatg atgacggtgt ttcccggcgc ggcccgtcgg ctgggcggcg
     4381 acatggaaaa gttcctctcg ctgggtcgct tccaacccac cgaccgcaac gagccgatca
     4441 acatgacggc cctggcgatc cgcatgagcc gttcgaccaa cggcgtgagc aagatccacg
     4501 ccggcgttgc acgccgcatg tggcaggggc tattccccgg gcgttccgca gaagaggtgc
     4561 cgatcaccca ggtcaccaac ggggtccacg tcccgagctg gatctcaccg atgatgcggc
     4621 gtctgctgga ccagtacctg gtgcccggct ggcacacgca cgagcggatc accgatcccg
     4681 ccacgtgggc ggccgtcgac cggattccgg acgcggagct gtgggccgtc cggcaggaat
     4741 acagcagaag gctggcgcgc tgggtccggt ccaggaccgt gaccgaccgc ctcacgcgcg
     4801 gcgatgtgat ggattacgcg ctgaaggcgg ccaactcctt cgacggcgag gcgctcacgc
     4861 tcgggttcgc gcgccggctg gcgacctaca agcggctgca cctgttgttc caggatcccg
     4921 ggagaatgct gagcctgctg gacgctccga gaccgataca ggtgctgatt gccggcaagg
     4981 cccatccccg cgacgacgac gccaagcgtg tcctgtcacg catgttcaat ctgaagtccg
     5041 acccgcgcat cggtggccgc gtcgccttcc tggaggacta ccacgtcggc ctggcgagca
     5101 tcctgaccag cgggtgcggt gtgtggatca acctgccgcg gccgccgctg gaagcgagcg
     5161 gcaccagcgg catcaaggcc gcattcaacg gcgtgctcaa cctgagcgtg ctcgatggct
     5221 ggtgggccga agcctacgac ggcagcaacg ggtgggcgat cgacggtggc gaggatgccg
     5281 acacggcggc caaggatgcc agggatgcgg ccgccttgtt cgacctgctg gaaagggaag
     5341 tcatcccgat gtactacgac cgcgacgcct ccggcgtccc gcacgcctgg gttgcgcgca
     5401 tcaaggcatc gcttcgcacc atcgggcccc ggttctgcgc gacgcgcatg ctggacgagt
     5461 acgcgcgcgg gatctacgcg gccgcgcaac cctgatcgca ccgtcggggt tccggcgcca
     5521 gcgcgcccgg cgacgccttg ttgctagact cgggcgcatg gggactctgc aacgctacgc
     5581 gcgccgcgcc gtcacgattc cggccgtcgc gctcgcgctg ctcgcgctgc ttgcggcgct
     5641 gccggtgctg ctgccgctcg cgttcgtcgt ggacctcgcg cgggcggcgg gacgacgcag
     5701 cttcgtgtcg gtgcgcatcg ttctgttcgg cgtgtgcttc ctcgccaccg aggtgctggg
     5761 tctcgctctg ctgttcggcg tgtggctttc atcggtcggg aggccggaac tccgcgccgc
     5821 gcgcacctgg cccgtgcagc gctggtacac ggcgatgcac tacgcggcat tgcgaacgct
     5881 cttccgcctg cgcttcgagg tggaaggcga cgcggcagcg acgcccggcc cgctgctcgt
     5941 gctggtgcgt catgcgagca tcatcgattc gctggtgccg gcggtcttca tcgcgaacgt
     6001 gcaccgcacg cgcctgcggt acgtgctgaa gcgcgagctg ctcgtcgatc cctgcctcga
     6061 cctggcgggc cactggctgc cgaatcgctt cgtcgcgcgc ggcggggtgg acacggcgcg
     6121 ggaagtggac tccgtgcggt cgctcaaggc cggcctcgag gcggacgaag gcgtgctgct
     6181 gtacccggaa ggcacgcgct tcacggagga gaagcgtcgc cgcgtgatcg agcggctcgg
     6241 cggcgccgcg cgcgagcgcg ccgagcgact ccagcacctg ctgccgatcc gtccaggcgg
     6301 ggtgctggcg ctcctcgatg cgccgcctgc gtgcgacgtg ctgttcatcg cccaccacgg
     6361 tctcgagggc ttcgcccacg tcgccgacgt cctgcgcggc gcactcgtcg ggcgcaccgt
     6421 caggctgcgc ttctggcggg tgcccgccgc ctcgatccct gccggtgcag agaagcggcg
     6481 cgcgtggctc gccgacgagt ggcagcgcat ggacgactgg ctctcggcgg aggaagaccg
     6541 ggatcgtaca ggcctgccag cggtaaaata gtcgtatgag cgacaccatc cgtctcgatg
     6601 agaccatcac cgtcgaccgt cccctgcacg aggtgttcgc gtacatctcc gagttctccc
     6661 gcatcgaaga gtgggacccg gccgtggcgc gcggccagcg cctcaccgaa ggcccgctcg
     6721 gcgtcgggag ccagttccgc atcgacatga aggccggctt ctcgctgcgc tacacggtca
     6781 tcgagtggga gcgggaccgg cgcctgctca tgaccgtcga ttcgaagccg ttcaccgcgc
     6841 gcgaggagat cttcttcgcc aggaccgccg ccggcacgca ggttcgctac gtcgcgacct
     6901 tcgagttcgt cgcgccgctg gcgcgcatcc tgaacacatt cccgggattg atggaccggg
     6961 tcggcaagag cgccatggcg ggaatgcggc gcgccctgga agaccgcttc gagccaccgg
     7021 gtgaagacgc ctggacggcg ctcgccgacc gcctgctcgt gcccggcctg gtgggcttca
     7081 cccgcttcgg ctaccgcgcc gcgcgcaagc attggaaccc cgtgtccgcg tacctgcgtg
     7141 accgccatgt cgtgatcacc ggagcgacct ccgggctcgg tctcgcgacg gcgacgaccc
     7201 tggccgcccg cggtgcggaa ctcacgctgg ttgcacgcga ccgcacgaaa gcgaacggcg
     7261 tggcgcgaga cctgaggcgc cgcttcggca accccagggt ccacgtcgag attgccgacc
     7321 tgagcctgct tgaagacgtg cgcgcgctgg tggcgcgctt gctcgcgaag ggccgggcga
     7381 tcgacgtgct ggtcaacaat gccggagcgc tgatcaatcc gcgcgaggag accgcggagg
     7441 gactggagaa gagcttcgcg ttgctgctcg ccggcccgta cgtgctcacg gaggggctgc
     7501 ggcccctgct caagcaggca gccgctgggc gcggtgtcgc gcgggtggtc aacgtgctgt
     7561 ccggaggcat gtacgcgcag aagatccacg tcgaggacct gcagagcgag cagggcaagt
     7621 actccggcgc gatcgcgtat gcgcgcgcca agcgtggact g
//
LOCUS       DAOMFD010000093         7595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3228_length_7595_cov_35.154775, whole genome shotgun sequence.
ACCESSION   DAOMFD010000093 DAOMFD010000000
VERSION     DAOMFD010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3228_length_7595_cov_35.154775"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="PL152_06755"
     CDS             <1..324
                     /locus_tag="PL152_06755"
                     /inference="COORDINATES: protein motif:HMM:NF015575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HQR49017.1"
                     /translation="MQYGVFAANYPLTEDDFESRQLPAMVRPYSAKLFGLTIEPHRLR
                     HIRNERRPNSRYASAAQCEYEVRSAEALFQRYGVPHLNTTECSIEEIASRVIDRMGIE
                     RRLRP"
     gene            431..877
                     /locus_tag="PL152_06760"
     CDS             431..877
                     /locus_tag="PL152_06760"
                     /inference="COORDINATES: protein motif:HMM:NF018552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 domain-containing protein"
                     /protein_id="HQR49018.1"
                     /translation="MSFVSLMTLYESNYIRLRGLVGDVRALAGRRVSQAPADCDLHLE
                     VVEHSPYTSVLRLTYLFEDPCAVVADPDLEVRVYHDARLAEASHCARWVRHPGLESIR
                     SRLSAALGERWLRNMLLNKWLDYCVDRGHRFAAQPAGVGDLAHEPA"
     gene            864..1901
                     /locus_tag="PL152_06765"
     CDS             864..1901
                     /locus_tag="PL152_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49019.1"
                     /translation="MNLRDDFQRLMQRLAGRRPSAEDERLVALFRNRAELKKELSTLA
                     DERHRLLDRLKLQEGATMKVEEQLATLEAYLGRPEEASKNLAYFHLKGLWRAATKRLE
                     GFASELEQQQKDRERRQQLGDFDRAKRARLGDVDRELVEARVLADQLQAEQKLAQQRL
                     ASLTGFWNYFRRRSLEEAIAERSARVEAALTVVTDLADARHAAEAEAPPTFESLSVEG
                     RRAVNLAIIACAESLCERLAPGGIVDLARQTTMRRVYETNYGSREQCQALIDAAAKAL
                     ADLEKQPVDLVDIKTRTDRLRRSAVYRADSDTIPAQDSVRPPAPADPGGVQAPHVLVD
                     EYFDIYRILLR"
     gene            complement(1915..5016)
                     /locus_tag="PL152_06770"
     CDS             complement(1915..5016)
                     /locus_tag="PL152_06770"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49020.1"
                     /translation="MRHTRIALERPVTTVMLALAVLAVGVISGRLLRLEAMPDIAFPG
                     MRVVIPYPGSTPEEMEQLVVRPVEEALATLSGIEEIDASAQSDQAQFDIQFNWDRDVD
                     AAEFEVRTKLDSIRPQLPAGADRLLMFAFSPSDQPIVVIRISADEDLTDQYDMLEKYL
                     QRPIQRLEGVARVELQGVQPREVRVLVDPNRMAAYGVDVRALRTLLEQSNFSVSAGEI
                     TENGSRFTVRPIGEFRSIQDVRELIVAPGVRVGDLAQVELVAPELPVGRHMDGRKAVG
                     IDVFKSTQANVVDVADRVIAAVNDARKVPQLQGIQILVVENQADSIRESLRELRNAGM
                     IGAVLSFFMLLFFLRHLPTTLIVSLAVPASLLVTLGAMYFFGLSLNILSMMGMLLAIG
                     MLVDNSVVITESIFRHRQLMPGRPLEATLAGVKEVGVATLAGTFSTTIVFLPLVFGEK
                     NQMSIFLVHVAVPIVVAMLASLVVAQTLIPMLAARMPPPPVVAAGSWFGRLQSRYQRS
                     LDWALGHPKLMALFTVLILLSPVPLFALKLAKVDPFPQEASRTLMLVYHLEGSHPMQQ
                     VEQAVDRVEAYLKAHKEEFDLETYYTFWLNDEANTRLYLTPKDRAKLPAREVMDRVMA
                     GMPEIIIGKPSFQFDDATSGSTSFSLQLSGESTERLAEISREVAHRLSSLPGLEAVRS
                     EAGTGEQEVQVVVNRDRAAQLGLTTQEIAMTVAGAMRGDKLPELRTSDRELTMRLAFR
                     ESDRQSVEDLARVPVMLPDGSRTELGAVADFTVQPSDREIKRVNRLTTVVINANLAKG
                     TTMDEVRKRVEPIMNDYPLPPGYSWKFGRGFDENDKAMQIMAQNMILAIVLIFLVMAS
                     LFESALYPLSIITSILFAIVGSIWFLTLTGTAITMMAMIGFMVLIGVVVNIGIVLIAH
                     VIDLRAAGLSRHDAILQAGRDRLRPILMTVLTTLLGLTPLAIGDAQVGGGGQGPAYYP
                     MARAIMGGLAFSAVVSLIIVPMFYVWFDDLNVWRRRVFGRAATATAVATPPSEVPEPG
                     PAS"
     gene            complement(5025..>7595)
                     /locus_tag="PL152_06775"
     CDS             complement(5025..>7595)
                     /locus_tag="PL152_06775"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49021.1"
                     /translation="LSGGRLEQGMQRFLVRTLNEFESIEQMANAIIANRDGQPVYLKD
                     VATVTRGYKDREAITRVDGREAIELAVYKEGDANTVQLAKAVRDRLEKLDKSLPEGTE
                     VRTVYDQSTFIAAAIGEVKSAALIGGLLAILVLYAFLRDARATLISGIAIPVSVLGTF
                     VLMYAFNLSLNIMSLGGIALAVGMLVDNAVVVLESIVRKQEHGMERREAARRGTAEVA
                     MAVTAATLTSVAVFFPMVFITGVAGQLFRDQSLTVTFALAFSLVVALTLVPMLAAGRS
                     PRLAASAAAAVSREPRRYEKVLVSLAGLFRTAFAWVSRACAVALSPIVRVTQAVNRWA
                     DDRYPTAIRWALAHPGRTIGSAVALFVFTVAVIVPRLGTELIPQMSQGEFNVDLRLAP
                     GTPLEQTDRSVQAAQQASSRLGNVALAYSVAGTGNRLDANPVDAGENTGRLSITLQKG
                     SKRSDEETAMARMRESLDNLPGVQYRFSRPALFSMSTPLEVVVSGYDLDRLKSAADQV
                     RARMLADKRFADVKTTVEAGNPEIQIVFDQERAAQLGLVVRDVADRLVNSVRGEVATR
                     YKLRDKQIDVLVRSVDNRAASIEEVRNLIVNPGSEYPVPLSAVADVQLAVGPAEVRRL
                     GQERVAVISANLAQGDLGSAIEALQGIVKAVELPVGTTAFLSGQSEEMDDSFRSLQFV
                     LLLAIFLVYLVMASQFESLVHPFVILLTIPLAIIGAVWALWLTHTTVNVVAYIGLIML
                     AGIVVNQSIVLIDAVNQARERGLGKHDAIVEAGRLRLRPILITKLTTILGLLPMALGI
                     GEGAEVRAPMAITVIGGVLLTTFLTLLVIPVVYAVLDRKQYPVHATDAATPSAEPAAG
                     "
BASE COUNT         1223 a         2590 c         2660 g         1122 t
ORIGIN      
        1 atgcagtacg gcgtgttcgc cgcgaactat ccgctcaccg aggacgactt cgagagccgc
       61 cagctgccgg cgatggtgcg gccgtactcg gcgaagctgt tcggcctgac gatcgagccg
      121 caccggctgc gccacatccg caacgagcgg cgcccgaaca gccggtacgc ctcggcggca
      181 cagtgcgaat acgaagtgcg cagcgccgag gcgctgttcc agcgctacgg ggtgccgcac
      241 ctgaacacca cggagtgctc gatcgaggag atcgcgagcc gggtcatcga ccgcatgggc
      301 atcgagcgcc ggctgcgtcc ttgaggcgcg ccgaggctgt ggcgcacgtc acggttgcag
      361 aaaaaccctg tgttatagtc gggccatgat cgcggacagt tgctgcgtga cgtcgtggcg
      421 ggcgaggccg gtgagcttcg tcagcctgat gacgctctac gagagcaact acatccgcct
      481 gcgcggcctg gtgggcgatg tccgcgcgct ggccgggcgc cgcgtgtcgc aggcaccggc
      541 cgactgcgac ctgcacctcg aagtggtcga gcacagcccg tacacgagcg tgctgcgcct
      601 gacctacctg ttcgaggacc cgtgtgcggt cgtcgccgac ccggacctcg aggtgcgcgt
      661 gtaccacgat gctcgcctcg cggaagcgag ccactgcgcc cgctgggtcc gccaccccgg
      721 cctcgagtcg atccgctcgc gcctgtccgc cgccctcggc gagcgctggt tgcgcaacat
      781 gctgctgaac aagtggctcg actactgcgt cgatcgcgga catcgctttg cggcgcagcc
      841 cgcgggagtc ggcgacctcg cgcatgaacc tgcgtgacga cttccagcgc ctgatgcagc
      901 ggctggcggg ccgccgtccc tccgccgagg acgagcggct cgtggcgctg ttccgcaacc
      961 gcgccgagct caagaaggag ctgtccacgc tcgccgacga gcggcatcgc ctgctcgacc
     1021 gcctcaagtt gcaggaaggc gcgaccatga aggtcgagga gcagctggcc accctcgagg
     1081 cctatctcgg ccgaccggag gaagccagca agaatctcgc ctacttccac ctgaaaggct
     1141 tgtggcgcgc tgcgacgaag cgcctcgagg gattcgcctc cgagctcgag cagcagcaga
     1201 aggaccgcga gcggcgccag cagctcggcg acttcgatcg tgccaagcgc gcccggctcg
     1261 gggacgtcga ccgtgagctg gtcgaggcac gggtactcgc cgaccagctg caggccgagc
     1321 agaagctggc ccagcagcgg ctggcgtcac tcaccgggtt ctggaactac ttcaggcggc
     1381 gcagcctcga ggaagcgatt gccgagcgtt cggccagggt cgaggccgcg ctcaccgtcg
     1441 tgaccgacct cgccgacgcg cggcatgcgg ccgaggccga ggcaccgccc acgttcgagt
     1501 cgctgagcgt cgaggggcgt cgcgccgtca acctcgcaat catcgcctgc gcagagtcgc
     1561 tgtgcgagcg gctggctccg gggggcatcg tggacctcgc gcggcagacg acgatgcgcc
     1621 gtgtgtacga aacgaactac ggcagccgcg agcagtgcca ggcactgatc gacgccgcgg
     1681 cgaaggcgct cgcagacctc gagaagcagc cggtggacct ggtggacatc aagacccgca
     1741 ccgaccgcct gaggcggtcg gccgtctatc gcgcggattc cgacaccatc ccggcgcagg
     1801 acagcgtgcg accgccggcg ccggcggacc cgggcggcgt ccaggcgccg cacgtcctgg
     1861 tcgacgagta cttcgacatc taccgaatcc tgctgcgctg aacgcgcccg cggctcagct
     1921 cgcgggtccg ggctccggca cctcgctcgg tggcgtcgca acggcggtcg cggtcgcagc
     1981 gcggccgaat acccgcctgc gccagacatt caggtcgtcg aaccaaacgt agaacatcgg
     2041 cacgatgatg agcgagacga ccgcggagaa ggcgaggccg cccatgatgg cgcgggccat
     2101 cgggtagtac gccggtccct ggccgccgcc gccgacctgc gcgtcgccga tggcgagcgg
     2161 cgtgaggccg agcagcgtgg tgagcaccgt catcaggatg ggtcgcagcc ggtcgcggcc
     2221 ggcctgcagg atcgcgtcgt ggcgcgagag cccggccgcg cgcaggtcga tgacgtgcgc
     2281 gatgagcacg atgccgatgt tcaccaccac gccgatcagc accatgaagc cgatcatggc
     2341 catcatggtg atggcggtcc cggtcagcgt caggaaccag atcgagccga cgatggcgaa
     2401 caggatcgag gtgatgatcg agagcgggta gagcgcggac tcgaagagcg acgccatgac
     2461 gaggaagatc agcacgatgg ccaggatcat gttctgcgcc atgatctgca tcgccttgtc
     2521 gttctcgtcg aagccccggc cgaacttcca cgagtagccg ggcggcagcg ggtagtcgtt
     2581 catgatcggc tcgacgcgct tgcgcacctc gtccatggtc gtgcccttgg cgaggttggc
     2641 gttgatcacg accgtcgtga gccggttgac gcgcttgatc tcgcggtcgc tcggctgcac
     2701 cgtgaagtcg gccaccgctc caagctcggt gcgtgagcca tcgggcagca tcaccggcac
     2761 ccgcgcgaga tcctcgacgg actgccggtc cgattctcgg aacgccagcc gcatggtcag
     2821 ttcgcggtcc gaagtacgca gttcgggcag cttgtcgccg cgcatcgcgc cggcgacggt
     2881 catcgcgatc tcctgggtcg tgaggccgag ctgggccgcg cggtcgcggt tcacgacgac
     2941 ctgcacctcc tgttcgccgg tgcccgcctc cgagcgcacc gcctcgaggc caggcaacga
     3001 cgacagccgg tgcgcgacct ccctggagat ctccgccagc cgctccgtgg actcgcccga
     3061 cagctgcagg ctgaacgacg tgctgccgct cgtcgcgtcg tcgaactgga agctgggctt
     3121 gccgatgatg atctccggca tccccgccat cacgcgatcc atcacctcgc gcgccggcag
     3181 cttcgcgcgg tccttcggcg tgaggtagag gcgagtgttg gcctcgtcgt tcagccagaa
     3241 cgtgtagtag gtctcgaggt cgaactcttc cttgtgcgcc ttcaggtagg cctcgacccg
     3301 gtccaccgcc tgctcgacct gctgcatcgg gtgcgagccc tcgaggtggt agaccagcat
     3361 gagcgtccgg ctcgcctcct gcgggaacgg gtccaccttg gccagcttga gcgcgaacag
     3421 cggcacgggc gacagcagga tcagcacggt gaacagcgcc atgagcttcg ggtgaccgag
     3481 cgcccagtcg agtgagcgct ggtagcggga ctgcagtcgc ccgaaccagg aacccgccgc
     3541 cacgacggga ggcggcggca tccgcgccgc cagcatcggg atcagcgtct gggccacgac
     3601 aagggacgcg agcatcgcga ccacgatcgg caccgcgacg tggaccagga agatcgacat
     3661 ctggttcttc tcgccgaaca ccagcggcag gaacacgatg gtggtcgaga aggtgccggc
     3721 cagcgtcgcc acgcccactt ccttcacgcc ggcgagcgtg gcctcgagcg gccgcccggg
     3781 catcagctgg cggtggcgga agatgctctc ggtgatcacc accgagttgt cgacgagcat
     3841 gccgatcgcg agcagcatgc ccatcatgct caggatgttg agcgacagtc cgaagaagta
     3901 catggcgccc agcgtcacca gcagcgaggc cgggaccgcc aggctcacga tgagcgtggt
     3961 cgggaggtgg cgcaggaaga acagcagcat gaagaagctc agcaccgcgc cgatcatgcc
     4021 ggcgttgcgc agctcgcgga gcgactcgcg gatgctgtcg gcctggttct cgacgaccag
     4081 gatctggatg ccctgcagtt gcggaacctt gcgggcgtcg ttgaccgccg cgatgacccg
     4141 gtcggcgacg tccacgacgt tggcctgagt cgacttgaag acgtcgatgc cgaccgcctt
     4201 gcggccgtcc atgtgacgcc cgacgggcag ctccggcgcg acgagctcga cctgcgcgag
     4261 gtcgcccacg cggaccccgg gcgcgacgat cagttcgcgc acgtcctgga tgctgcggaa
     4321 ctcgccgatc ggccgcaccg tgaagcgcga gccgttctcg gtgatctcgc cggcgctcac
     4381 cgagaaattg ctctgctcga ggagggtgcg cagcgcgcgc acgtcgacgc cgtaggccgc
     4441 catgcggttc gggtccacca gcacccgcac ctcgcgcggc tgcacgccct gcaattcgac
     4501 gcgcgccacg ccctccagcc gctggatcgg ccgctgcagg tacttctcca gcatgtcgta
     4561 ctggtcggtc aggtcctcgt ccgccgagat gcggatcacc acgatcggct ggtcggacgg
     4621 gctgaacgca aacatgagca gccggtccgc gccggccggc agctgcgggc ggatcgagtc
     4681 gagcttggtg cgcacctcga actccgcggc atccacgtcg cggtcccagt tgaactggat
     4741 gtcgaactgc gcctggtcgg actgcgcgct ggcgtcgatc tcctcgatgc cggagagcgt
     4801 cgcgagcgct tcctcgaccg ggcgcaccac gagctgctcc atctcttcgg gcgtggaccc
     4861 ggggtagggg atgacgacac gcatgcccgg gaaggcgatg tccggcatgg cctcgagccg
     4921 cagcaggcgg cccgagatca ccccgaccgc aagcacggcc agggcgagca tcaccgtcgt
     4981 gacggggcgt tcgagcgcga tccgggtgtg gcgcatggcg agcctcagcc ggcggcgggt
     5041 tcggcggacg gggtcgcggc gtcggtcgcg tgcacggggt actgcttgcg gtcgagcacc
     5101 gcgtagacga cggggatcac gagcagcgtc aggaacgtcg tgagcagcac gccgccgatc
     5161 acggtgatgg ccatcggcgc gcgcacctcg gctccctcgc cgatgccgag cgccatcggc
     5221 agcaggccca ggatcgtcgt cagcttggtg atcaggatcg ggcgcaggcg cagcctgccg
     5281 gcctcgacga tggcgtcgtg cttgccgagg ccgcgctcgc gcgcctggtt caccgcgtcg
     5341 atcagcacga tcgactggtt cacgacgatg ccggccagca tgatgaggcc gatgtaggcc
     5401 acgacgttca ccgtggtgtg cgtcagccac agcgcccaga ccgcgccgat gatcgcgagc
     5461 gggatggtga gcaggatgac gaacgggtgc accagcgact cgaactggga ggccatcacg
     5521 aggtagacga ggaagatcgc gagcagcagc acgaactgca gcgaccggaa cgagtcgtcc
     5581 atctcctcgc tctggccgga caggaacgcc gtggtgccga ccggcagctc gaccgccttg
     5641 acgatgccct gcagcgcctc gatcgcggaa cccaggtcgc cctgcgcaag gttggcggag
     5701 atgaccgcca cgcgctcctg gcccaggcgg cgcacctcgg ccgggccgac cgccagctgg
     5761 acgtcggcga ccgcgctcaa cggcaccggg tactcgctgc ccgggttgac gatcaggttg
     5821 cgcacctcct cgatcgacgc cgcgcggttg tccacgctgc gcacgaggac gtcgatctgc
     5881 ttgtcgcgca gcttgtagcg cgtcgccacc tcgccgcgca cgctgttcac gagccggtcg
     5941 gcgacgtcgc gcaccacgag gccgagctgc gcggcacgct cctggtcgaa cacgatctgg
     6001 atctcggggt tgccggcctc gacggtcgtc ttcacgtcgg cgaagcgctt gtcggcgagc
     6061 atgcgggccc gcacctggtc ggcggcgctc ttcagccggt cgaggtcgta gccggagacg
     6121 accacctcga gcggcgtcga catgctgaac agggcggggc ggctgaagcg gtactgcacg
     6181 cccgggaggt tgtcgaggct ctcgcgcatg cgcgccatgg cggtctcctc gtcggagcgc
     6241 ttagagccct tctgcagcgt gatgctcagg cggccggtgt tctcgccggc gtcgaccggg
     6301 ttggcgtcca ggcggttgcc ggtgccggcc accgagtagg cgagcgcgac gttgccgagc
     6361 ctggaactcg cctgctgcgc ggcctgcacc gagcggtcgg tctgctcgag cggcgtcccc
     6421 ggtgcgagcc gcaggtcgac gttgaattcg ccctgggaca tctgcggaat gagttcggtg
     6481 ccgagacgcg gcacgatcac cgccaccgtg aacacgaaca gcgccacggc cgagccgatg
     6541 gtccggccgg gatgcgcgag cgcccagcgg atcgccgtcg ggtaacggtc atcggcccag
     6601 cgattcacgg cctgcgtgac gcgcacgatc gggctcagcg ccacggcgca ggcgcgcgag
     6661 acccaggcga aggccgtgcg gaacagtccc gccaggctca ccaggacctt ctcgtaccgc
     6721 ctgggctcgc ggctcacggc ggccgccgcc gatgccgcga ggcgcgggga acggccggcc
     6781 gccagcatcg gcaccagcgt gagggcgacg acgagggaaa atgcgagcgc gaaggtgacg
     6841 gtcagcgact ggtcgcggaa cagctggccc gccacccccg tgatgaacac catcgggaag
     6901 aacaccgcga cggaggtcag ggtcgcggcg gtcaccgcca tcgccacctc ggccgtgccg
     6961 cggcgcgccg cttcgcggcg ctccatgccg tgctcctgct tgcgcacgat gctctcgagc
     7021 acgaccacgg cgttgtcgac cagcatgccg accgcgagcg cgatgccgcc gagcgacatg
     7081 atgttgagcg agaggttgaa cgcgtacatc agcacgaagg tgccgagcac cgacaccggg
     7141 atggcgatgc cgctgatgag cgtggcgcgc gcgtcgcgca ggaacgcgta gagcacgagg
     7201 atcgcgagca ggccgccgat cagggcggcg ctcttcactt cgccgatggc cgccgcgatg
     7261 aacgtggact ggtcgtaaac cgtacggacc tcggtgccct cgggcaggct cttgtcgagc
     7321 ttttcgaggc ggtcgcgcac ggccttggcg agctgcaccg tgttggcgtc gccctccttg
     7381 tagaccgcga gctcgatggc ctcgcggccg tcgacgcgcg tgatggcctc gcggtccttg
     7441 tagccgcgcg tgaccgtggc cacgtccttc aggtacacgg gctggccgtc gcggttggcg
     7501 atgatggcgt tggccatctg ctcgatgctc tcgaactcgt tcagcgtgcg caccaggaag
     7561 cgctgcatgc cctgctcgag gcgtcccccc gacag
//
LOCUS       DAOMFD010000094         7580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3235_length_7580_cov_33.050365, whole genome shotgun sequence.
ACCESSION   DAOMFD010000094 DAOMFD010000000
VERSION     DAOMFD010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3235_length_7580_cov_33.050365"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            115..1500
                     /locus_tag="PL152_06780"
     CDS             115..1500
                     /locus_tag="PL152_06780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF020872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HQR49022.1"
                     /translation="MIYKSFLAALIACASLAAAAQALDPETPVAQPSPLATAPAASPS
                     GAAAPATPAAPRGVSLDRVVAVVNDGVVLQSSLDQQVQSVTERLQQAGQQLPPRDVLR
                     QQVLERLILQEIQVQRAAKAGIQVSDEELNQALTDIAKRNNIKFSDLPAALEAQGMDY
                     RTYREEVRREMTLGQLRQRDVYARIYVSPHEIDLCVSKALAAPEDRLEYDVAHILVAI
                     PASATPQQVDERTARAQGVYERARRGEDFSDLAVAYSDAGTALDGGKLGWRKANQLPS
                     VIADVIPGMKAGEVTEIIRTPSGLNIFKLVDTRSTQQAALVSQVHTRHILMQTNAIED
                     DETVRQKLERIRDRVLKGEDFAAIASVSSADPGSATRGGDLGWTSPGTFVPEFEKTVD
                     RLDINEISEPFKTQFGWHIVQLLGRRTYDASEDTVRNKCVMQLRESRADEETELWLRR
                     LRDEAYVEYRP"
     gene            1497..2486
                     /gene="pdxA"
                     /locus_tag="PL152_06785"
     CDS             1497..2486
                     /gene="pdxA"
                     /locus_tag="PL152_06785"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HQR49023.1"
                     /translation="MSGSRRLPTLAVTTGEPAGIGPDLCAAVAAGRWDCRIVLLGDRS
                     LLGRVRGTRRRMSSLPDYDPASPARRRVEILHVPLAAPCTPGRLDPANAGYVLRLLDR
                     ACDGCRSGEFSAMVTAPVHKGAINDAGEAFTGHTEYLAERCGADRPVMLLAGGGLRVA
                     LATTHLPLAHVPAAITGELLDSVLRVLDADLRRLYAIRRPRILVCGLNPHAGEGGHLG
                     REEIDVIAPALERLRRAGLSITGPVPADTAFLPARLAECDAVLAMYHDQGLPVLKREA
                     FADAVNVTLGLPIVRTSVDHGTALELAGTGRADPGSLLAAVRLAARFSGRRRR"
     gene            2483..3259
                     /gene="rsmA"
                     /locus_tag="PL152_06790"
     CDS             2483..3259
                     /gene="rsmA"
                     /locus_tag="PL152_06790"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HQR49024.1"
                     /translation="MNRAKRRYGQHFLHDRGTIERIVRAVAPASGDRIVEVGPGRGAI
                     TAPLLAIVGEMDVVEIDAEVIPRLEERCAGIGRLRVHVADALEFDFRALRGAGPPLRL
                     VGNLPYNVSTPLLFHFVSQLDAIRSMHFMLQKEVVARMAAQPGTRDYGRLTVMLAPWV
                     RVEPLFDIGTGAFSPPPKVVSTFFALHPHVTPPYAIADHGAYARVVAAAFSQRRKTLR
                     NSLAGLLEPAAIAAAGVDPRARPETLGPDQFAALAARLSS"
     gene            3372..3809
                     /locus_tag="PL152_06795"
     CDS             3372..3809
                     /locus_tag="PL152_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HQR49025.1"
                     /translation="MRDYRNILICLDLTEDSEKIAERAKAMAALSGGVVTLLHVVEYV
                     PVEPMGEALLPAVQIEGELVERATARIGELAVKLGLDKSDRLVLTGNIKSEVVRVAQE
                     RGVDLIVIGSRERHGVSILFNFTEDTVLHAAPCDVLAVRLHKA"
     gene            3829..4212
                     /gene="apaG"
                     /locus_tag="PL152_06800"
     CDS             3829..4212
                     /gene="apaG"
                     /locus_tag="PL152_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HQR49026.1"
                     /translation="MSPEESHRIRVDVTTHYVEEQSSPGDGRFVFSYTITIRNEGSVP
                     ARLLTRHWVITDANGKVQEVVGEGVVGEQPHLQPGEGFRYSSGTILETPVGAMQGTYR
                     MVADDGEHFDAPIAPFTLAMPGLLH"
     gene            4217..5056
                     /locus_tag="PL152_06805"
     CDS             4217..5056
                     /locus_tag="PL152_06805"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008803 -
                     bis(5'-nucleosyl)-tetraphosphatase (symmetrical) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="symmetrical bis(5'-nucleosyl)-tetraphosphatase"
                     /protein_id="HQR49027.1"
                     /translation="MATYAVGDVQGCAAELERLLERLAFSPARDRLWFVGDLVNRGPR
                     SLDVLRMVTRLGDAAVVVLGNHDLHLLASARGHTGLRAQDESLRPVLDAPDRESLLDW
                     LQSRPMLHHDPALDIHLLHAGLPPQWDVALARRCARELEAALRSDRSGVLFDRMYGNQ
                     PDLWRDDLEGVDRLRFITNALTRLRACDAAGRMLLSFKGPVAKLPPGATPWFRASERR
                     SAGARIVCGHWSALGYLRESGVLALDTGCVWGGALTAERLDAPSAPVQVASEQPRTEF
                     TEG"
     gene            complement(5135..5812)
                     /locus_tag="PL152_06810"
     CDS             complement(5135..5812)
                     /locus_tag="PL152_06810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49028.1"
                     /translation="MKFLRYPVVAASLALLSSSCLAQAQPPSRPEPPGAIPAAASGEA
                     TAPVMAVWVEQRIHFPYMAFTAYYSCDGLERKVEQVLAAIGARPGFTVTARGCMNPRR
                     GAELSPSVDIVVAMPQAATPETLAAVAKAASTQELAARAGGKPAPAAEATAEFPARLR
                     RIDFRDSPTGLLQPGDCELMEGMRDWVFKPLGAKIVVDRMSCVPHTLNIGIIVLSIDV
                     LEPVPQP"
     gene            5977..>6212
                     /locus_tag="PL152_06815"
     CDS             5977..>6212
                     /locus_tag="PL152_06815"
                     /inference="COORDINATES: protein motif:HMM:NF013703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQR49029.1"
                     /translation="MQDLTQGPIRQHLVRMAVPMAIGMLMQTLYFLVDLYFVGRLGDA
                     ALAGVGAAGTLTMVVVALTQVLGVGTVALIAQAVG"
     assembly_gap    6213..6312
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6313..6588
                     /locus_tag="PL152_06820"
     CDS             <6313..6588
                     /locus_tag="PL152_06820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQR49030.1"
                     /translation="YLSIISWNFVAAGINFSCSGMFQALGNTVPALVSSGGRLVTFVL
                     PILWLARRPGIELRDFWYVSVASTALQAVTSLLLLRMVMRQKLRPSP"
     gene            6585..7106
                     /locus_tag="PL152_06825"
     CDS             6585..7106
                     /locus_tag="PL152_06825"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR49031.1"
                     /translation="MTAPVLETQRLRLRRLVPGDAGFMLELLNDPSFIEFIGDRGVRD
                     QAGARRYIEDGPVASHARYGYGMDLVELKVTAEPIGMCGLVRRNWHDLPEIGYAFLPR
                     HRSAGYAHEAAAAVLANALGALALPRVIAIVSPGNTRSIRVLEKIGLRYECEVTPPGE
                     QRAILQFTTVGAT"
     gene            complement(7084..>7580)
                     /locus_tag="PL152_06830"
     CDS             complement(7084..>7580)
                     /locus_tag="PL152_06830"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF012413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HQR49032.1"
                     /translation="SVTELCIVVAADEQGGIGRDGRLPWHLPDDLKRFKAITMGSPVV
                     MGRKTWDSIGRPLPGRHNIIVSRQAGLAIPGCTVAGSLETALQVAGDVPRVFVIGGAD
                     LYRLALPRAGTVHLTRVHAIAGADTFFPRLDAAEWEEVAREDCAADGRHAHDYSFVTL
                     RRRS"
BASE COUNT         1104 a         2661 c         2610 g         1105 t
ORIGIN      
        1 tggaactgaa gggcctgtcg agtgtcggag tggacaacga agctttcctg cgggaacaga
       61 ttcgtggata ctccgcgctc gccacggacc cccagtccca accctgagat tcccttgatt
      121 tacaagtctt ttctcgccgc cctgatcgcg tgcgcgagcc tcgcagccgc cgcccaggcg
      181 ctggacccgg agacgcccgt cgcgcagccg agcccgctcg cgaccgcgcc ggcggcgtcc
      241 ccgtccggcg ccgctgctcc ggcgacgccc gccgcgccgc ggggcgtgtc gctcgatcgc
      301 gtcgtcgcag tggtgaacga cggggtcgtg ctgcagagct cactcgatca gcaggtgcag
      361 tcggtgaccg agcgcctgca gcaggccggc cagcagctcc cgccgcgcga cgtgctgcgc
      421 cagcaggtgc tcgagcggct gatcctccag gagatccagg tgcagcgcgc ggcaaaggcg
      481 ggcatccagg tctccgacga ggagttgaac caggcgctca ccgacatcgc caagcgcaac
      541 aacatcaagt tcagcgacct gccggcggcg ctcgaggcgc agggcatgga ctatcgcacc
      601 taccgcgaag aggtgcgccg cgaaatgacc ctgggacagt tgcgtcagcg cgacgtctac
      661 gcgcgcatct acgtgtcgcc gcacgagatc gacttgtgcg tgtccaaggc gctggcggca
      721 ccggaggacc ggctcgagta cgacgtcgcg cacatcctcg tggccatccc ggcgtcggcg
      781 acgccgcagc aggtcgatga acgcaccgcg cgtgcacagg gcgtctacga gcgagccagg
      841 cgcggcgagg atttttccga cctcgccgtc gcatactccg acgcgggcac ggcgctcgac
      901 ggcggcaagc tcgggtggcg caaggctaac cagctgccgt cggtcatcgc cgacgtcatt
      961 cccgggatga aggccggcga ggtgacggag atcatccgca ccccgagcgg gctcaacatc
     1021 ttcaagctgg tggacacgcg cagcacgcag caggcggcgc tggtgtcgca ggtgcacacg
     1081 cgccacatcc tgatgcagac caacgccatc gaggacgacg aaacagtccg gcagaagctc
     1141 gagcggatcc gcgaccgggt gctcaagggc gaggacttcg cggccatcgc gtcggtctcc
     1201 tcggccgacc ccggttcggc gacccgcggc ggcgacctcg gctggaccag cccgggcacg
     1261 ttcgtgcccg agttcgagaa gaccgtcgac cggctggaca tcaacgagat cagcgagccg
     1321 ttcaagacgc agttcggctg gcacatcgtg cagctcctcg gtcgtcgcac ctacgacgcc
     1381 tcggaggaca cggtgcgcaa caagtgcgtg atgcagctgc gtgaatcgcg cgccgacgag
     1441 gagacggagc tctggttgcg tcgccttcgc gacgaggcgt acgtcgaata ccggccgtga
     1501 gcggctcccg gcggctgccg acgctggcgg tgacgaccgg cgagccggcc gggatcgggc
     1561 cggacctctg cgcggcagtt gccgcggggc gctgggattg ccgcatcgtc ctgctcggcg
     1621 accgcagcct gctcgggcgc gtccgcggca cgagacggcg aatgagttcg ctgcctgact
     1681 acgacccggc gagcccggca cggcgccgcg tggagatcct gcacgtgccg ctggcggcac
     1741 cgtgcacccc cggccgtctc gatcccgcga acgcgggcta cgtgctgcgc ctgctcgatc
     1801 gggcgtgcga cggctgtcgg tcgggcgagt tctcggcgat ggtgacggca cccgtgcaca
     1861 agggcgcgat caacgacgcc ggcgaggcct tcaccggcca cacggaatac ctggccgaac
     1921 gctgcggcgc ggatcggccc gtgatgctgc tcgccggtgg cggcctgcgc gtcgcgctcg
     1981 cgacgacgca cctgccgctg gcgcacgtgc cggcggcgat aaccggggag ctgctcgact
     2041 cggtgctgcg cgtcctggac gccgacctga ggcgcctcta cgcgattcgc cggccacgca
     2101 tcctggtgtg cgggctcaat ccgcacgccg gtgaaggtgg ccacctcggc cgcgaggaaa
     2161 tcgacgtcat cgcaccggcg ctcgagcgcc tgcgccgcgc gggcctgtcg atcacggggc
     2221 ccgtgcctgc cgacacggcg ttcctccccg cgaggctcgc ggagtgcgac gcggtgctcg
     2281 cgatgtacca cgaccagggg ctgcccgtcc tcaagcggga ggcgttcgcc gacgcggtca
     2341 acgtcacgct cgggctgccc atcgtgcgca cgtccgtgga ccatggcacg gcgctggagc
     2401 tcgcgggaac gggccgcgcg gaccccggca gcctgctcgc cgcagtgcgc ctggccgcgc
     2461 gattcagcgg gcgccgccgc cggtgaaccg cgcgaagcgg cgctacggac agcacttcct
     2521 gcacgaccgc gggaccatcg agcgcatcgt ccgcgccgtc gcacccgcgt cgggcgaccg
     2581 gatcgtcgag gtcggcccgg gccgcggcgc catcaccgcc ccgctgcttg cgatcgtggg
     2641 cgagatggac gtcgtcgaga tcgacgccga ggtgataccc cgcctcgagg agcgttgcgc
     2701 cgggatcggc cggctgcgcg tgcacgtggc ggacgcgctc gaattcgact tccgcgcgct
     2761 gcgcggtgca ggcccgccgc tcaggctggt cggcaacctg ccgtacaacg tgtcgacgcc
     2821 gctgctcttt cacttcgtct cgcagctcga tgcgatccgc agcatgcact tcatgctgca
     2881 gaaggaggtc gtggcgcgca tggcggcgca acccggcacc agggactacg ggcggttgac
     2941 ggtgatgctg gcgccgtggg tccgcgtcga gccgctgttc gacatcggca ccggcgcgtt
     3001 ctcgccgccg cccaaggtcg tctcgacgtt cttcgcgctg cacccgcacg tcacgcctcc
     3061 ctatgcgatc gcggaccacg gtgcgtacgc ccgggtcgtc gcggctgcgt tctcgcagcg
     3121 gcgcaagacc ctcaggaatt cgctcgccgg cctgctggaa ccggccgcga tcgcggcggc
     3181 gggggtcgac cctcgggcgc ggcctgaaac cctcgggccg gaccagttcg cggccctggc
     3241 ggccaggttg agttcctgat cagcggcggc atacgcagcc acaggcatcg gaggctcccg
     3301 caattggtgg cgcggccccc cggacctata atcccggcgt agggacacgc acggacccgc
     3361 gggaggcatc gatgcgcgat tacaggaaca tcctgatctg cctggacctg acggaagaca
     3421 gtgagaagat cgccgagcgc gccaaggcga tggcggcgct gtcgggcggc gtcgtgacgc
     3481 tgctgcacgt ggtcgagtac gtcccggtcg agccgatggg tgaagcgctg ctgccggcgg
     3541 tgcagatcga gggcgaactc gtcgagcgtg ccaccgcgcg catcggcgaa ctggcggtga
     3601 agctggggct cgacaagtca gatcgcctcg tgctcaccgg caacatcaag tccgaggtcg
     3661 tgcgcgtcgc ccaggagcgc ggcgtggacc tgatcgtcat cggcagccgc gagcggcacg
     3721 gcgtctcgat cctgttcaac ttcaccgagg acacggtgct gcacgcggcg ccctgcgatg
     3781 tcctggcagt ccgcctgcac aaggcgtgag cgccgcggga gtgccccgtt gagcccggag
     3841 gaatcgcatc gcatccgcgt ggacgtgacc acgcactacg tcgaggagca gtcgagcccc
     3901 ggcgacggca ggttcgtgtt cagctacacg atcaccatcc gcaacgaagg gtccgtcccc
     3961 gcccgcctgc tgacgcggca ctgggtcatc acggacgcta atggcaaggt gcaggaagtg
     4021 gtgggcgaag gcgtggtcgg cgagcagccg cacctgcagc cgggcgaagg cttccgctat
     4081 tcgagcggga cgatcctcga gacgcccgtg ggtgccatgc agggcaccta tcgcatggtc
     4141 gcggacgacg gcgagcactt cgacgccccg atcgcgccgt tcacgctcgc gatgcccggc
     4201 ctgctgcact gaccggatgg cgacgtacgc cgtcggcgac gtgcagggct gcgccgccga
     4261 actcgagcgc ctgctcgagc gcctggcatt ctcccccgcc cgcgaccgcc tctggttcgt
     4321 gggcgacctc gtcaaccgcg gcccgcgctc gctcgacgta ctgcgcatgg tcacgcgcct
     4381 cggtgatgcc gcggtcgtcg tgctcggcaa tcacgacctg cacctgctcg cgtctgcgcg
     4441 cggccacacc gggctgcgag cgcaggacga atcgctgcgc ccggtcctcg acgcgcccga
     4501 tcgcgagagc ctcctcgact ggctgcagtc tcgcccgatg ctgcaccacg accctgcgct
     4561 cgacatccac ttgctgcacg cagggttgcc gccacagtgg gacgtggcgc tcgcacgccg
     4621 ttgcgcacgc gaactcgagg ctgccctgcg cagcgatcgc agcggcgtgc tgttcgaccg
     4681 catgtacggc aaccagcccg acctctggcg cgacgacctc gagggtgtcg accgccttcg
     4741 attcatcacc aacgccctga cgcgactgcg cgcctgcgat gcggccgggc gcatgctgct
     4801 ctccttcaag ggccccgttg ccaagctgcc gcccggcgcg accccctggt ttcgcgcgtc
     4861 ggaacggcgc tcggccggcg cccgtatcgt gtgcggacac tggtcggcgc tcgggtacct
     4921 gcgcgaatcg ggcgtgctgg cgctcgacac gggatgcgtc tggggcggcg cgctcacggc
     4981 cgagcggctg gacgcgccgt ccgcgccggt tcaggtcgca agcgagcagc cgcgcaccga
     5041 gttcaccgaa ggctgactgc tcgactccgc ccggcgagcg ggctggccgt accgttggtc
     5101 ccgcaaacgc gccaggccgc tgtcgccatt cgcgtcaggg ctgcggaacc ggctccagca
     5161 cgtcgatgga caatacgatg atgccgatgt tcagggtatg cgggacgcag ctcatgcgat
     5221 ccaccacgat cttcgcgccc agcggcttga agacccagtc gcgcatgcct tccatcaact
     5281 cgcagtcccc gggctgcagc agccccgtcg ggctgtcgcg aaagtcgatg cgtcgcagcc
     5341 tcgcggggaa ctcggcagtg gcctcggccg cgggcgccgg cttgccgccg gcgcgcgctg
     5401 ccagttcctg cgtcgacgcc gcctttgcga cggcggcgag cgtctccggc gtcgccgcct
     5461 gcggcatcgc aaccacgatg tcgacgctgg gcgagagttc ggctccgcgt cgcggattca
     5521 tgcagccgcg ggcggtcacg gtgaatcccg gacgcgcgcc gatggccgcg agaacctgct
     5581 ccaccttcct ctcgagcccg tcgcaagagt agtaggccgt gaatgccatg tacggaaagt
     5641 ggatccgctg ctcgacccag accgccatca cgggcgccgt cgcctcgcca ctggctgcgg
     5701 ccggaatcgc gccgggcggc tccgggcgtg acggcggctg cgcctgtgcg aggcacgagg
     5761 acgacagcag tgcgagactc gctgcaacca ccggatagcg caggaacttc atgtcgacct
     5821 ccccggcccc gaacgggggt cacgcgtata tactgccacg ctcgcgcaga ctgggtggcc
     5881 ggaccggttg cccgcagaac cagaacgcct gcgcacctgt tatcgacccg gccggcccgc
     5941 cgaagttcgc cgcagcgcgg gacgaggagt tcacgcatgc aggacctgac acagggcccg
     6001 atccgccagc acctggtgcg gatggcggtg cccatggcga tcggcatgct gatgcagacg
     6061 ctgtacttcc tggtcgacct gtacttcgtc gggcggctcg gcgacgcggc gctcgccggc
     6121 gtcggcgccg ccgggacgct caccatggtc gtggtggcgc tgacgcaggt gctgggcgtc
     6181 ggcaccgtcg cgctgatcgc gcaggcggtc ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6301 nnnnnnnnnn nntacctgag catcatttcc tggaacttcg tcgccgccgg catcaacttc
     6361 agctgctccg gcatgttcca ggcgctcggc aacaccgtgc cggcgctcgt gagcagtggc
     6421 gggcggctcg tcacgttcgt gctgccgatc ctgtggctcg cccggcgccc cggcatcgag
     6481 ctgcgcgact tctggtacgt ctccgtggcg agcacggcgc tgcaggccgt gaccagcctg
     6541 ctgctgctgc ggatggtgat gcggcagaag ctgcggccct cgccgtgacc gcgcccgtcc
     6601 tcgagacgca gcgcctgcgc ctgcggcgac tggtccccgg cgatgccggc ttcatgctgg
     6661 aattgctgaa cgacccgtcg ttcatcgagt tcatcggcga ccgcggggtg cgcgaccagg
     6721 cgggcgcccg gcgatacatc gaagatgggc cggtcgcgag ccacgcccgg tacgggtacg
     6781 gcatggacct cgtcgagttg aaggtaacgg ccgagccgat cggcatgtgc ggtctggtcc
     6841 gccgcaattg gcacgacctt ccggaaatcg ggtacgcctt cctgccgagg caccggtccg
     6901 cgggatacgc gcacgaagcc gcggcggcgg tcctggccaa cgcgctcggc gcgctcgcat
     6961 tgccgcgcgt gatcgcgatc gtgtcacccg gaaacacgcg atccatccgc gtgctcgaga
     7021 agatcggcct gcgctacgag tgcgaggtca ctccgcccgg cgagcagcgg gcgatcctgc
     7081 agttcacgac cgtcggcgca acgtgacgaa actgtagtcg tgggcgtggc gcccgtccgc
     7141 agcgcagtcc tcccgggcga cctcctccca ctccgccgcg tcgagccgcg ggaagaacgt
     7201 gtccgcgccg gcgatcgcgt ggactcgcgt caggtgcacg gtcccggcgc gcggcagcgc
     7261 caggcggtag aggtccgccc cgccgatgac gaagacgcgc ggaacgtcgc cggcaacctg
     7321 cagcgcggtc tcgagcgacc cagcgacggt gcatcccggg atcgccagcc ccgcctgccg
     7381 gctcacgatg atgttgtgcc gtcccggcag cggccggccg atcgagtccc aggtcttgcg
     7441 gcccatcacg accgggctgc ccatcgtgat cgccttgaag cgcttgaggt cgtcgggcag
     7501 gtgccaggga aggcgcccgt cgcgaccgat gccgccctgc tcgtcggccg cgacgacgat
     7561 gcagagttcc gtcaccgacc
//
LOCUS       DAOMFD010000095         7335 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3451_length_7335_cov_24.823214, whole genome shotgun sequence.
ACCESSION   DAOMFD010000095 DAOMFD010000000
VERSION     DAOMFD010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7335)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7335)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7335
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3451_length_7335_cov_24.823214"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1287
                     /locus_tag="PL152_06835"
     CDS             <1..1287
                     /locus_tag="PL152_06835"
                     /inference="COORDINATES: protein motif:HMM:NF012926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HQR49033.1"
                     /translation="ERFFEVDEPLAFLIGPDSAIEEHPLGLATTFLDATRAYWEDWVR
                     GLAIPFEWQQAVIRAAITLKLCTYEDTGAVLAALTTSVPESPDSGRNWDYRYCWLRDS
                     YLVIQALNRLGATRTMEGYLRFIKHTFGSDDIRRLQPLFSITGEPEVPERQVATLPGY
                     RGMGPVRVGNDAYKQDQHDVYGALILASAHSFCDERIGSPGDVALFNRLEPIGAKAVE
                     FWQGPDAGPWEFRGAVRSHTFSAAMCWAGCDRLARIAGRLALPARESHWRNAADGIRE
                     QVLSRSWNPTRRAFCSSFGGDHVDATALVLPDLGIVPPHDPRFLSTVEAIEHDLKDGD
                     WIYRYRHEDDFGVPATAFTICAFWYVNALAAVGRREEAREIFERLLARRNRLGLLSED
                     IDPRSGELWGNFPQTYSMVGIINSAVRLSRSWEEAM"
     gene            1300..3516
                     /locus_tag="PL152_06840"
     CDS             1300..3516
                     /locus_tag="PL152_06840"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
                     /protein_id="HQR49034.1"
                     /translation="MRWSAVRNIAIRYLVPIVAAVSLVAFVGVPYIDHILAGWFRSDV
                     QMRARLVMTSVEEPLGNLLDGAELDRARTYLGRVASDERLLGVVVCAPDGSRVVRTVL
                     VPDTVTCATDATQPSDGSTARTVGAGLVEISRFPLVTAAGHSFEVLIVHDLSFIDRRQ
                     TSARNFVLAFALLAACALAVLAGLGVRLLLNQWIRLLVGDISSKRFLDDAESSRASVP
                     VLMEVRQALRDLEASQRLEIEFQENWTPQALQQVVRESLESSPMLVVSNREPYIHNLD
                     AQGQPVVQVPASGMVTAIEPVMRACSGTWIAHGSGTADRLVVDRRDHVRVPPDDPSYE
                     LRRVWLTQPEEEGYYYGFSNEGLWPLCHLAYVRPAFREADWRQYIEVNGRFADVVAQE
                     ARKTEPVVLIQDFHFALLPRLIRERGIRATIALFWHIPWPNAETFGVCPWKREILLHM
                     LSADILGFHTRYHCQNFLATVDRFIECQIDQEHMTVTHQGHVCRVKAYPISIEWPPRW
                     LAGAPSPENARTNVKRRWGIGPDVRIGVGVERWDFTKGIVERFQSFETLFEKQPEWKG
                     RLTFLQVAAPSRSKLPAYQALQQQTLDELHRINARFGTPDWQPIMLIGQHQEPAQVFE
                     LYRAADFCLVNSLHDGMNLVAKEFVAARDDEDGVLVLSSFTGASRELVEALLVNPFDV
                     SETARAIVTALEMPREERRKRMQLMRRTISEANVYRWAGRMLMDVARIRQRQTLTR"
     gene            complement(3625..4698)
                     /locus_tag="PL152_06845"
     CDS             complement(3625..4698)
                     /locus_tag="PL152_06845"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HQR49035.1"
                     /translation="MGEWFPKVPSAWLSSHGTDLVLAAAVGAVLLAVLWALRSVARRH
                     YRRFERTGQTELLEIPLQVASRTTWIFMAVASLCGGLALGDFGAHAESLLARTFAVAL
                     FWQSGVWATTAVGAWLDQRRKVALESDKAAAGTIGIIRFLARMVVWALVVLLTLDNLG
                     FDITALVAGLGIGGVAVALAVQNVLGDLFASLSIALDQPFVIGDFLVVGDLMGTVEYV
                     GIKSTRLRSLSGEQIVMSNADLLGGRLRNYGRMRERRVVFTVGVTYETPRALLEKVAT
                     VLRSAIERRDGVRFDRAHFAKFGPYSLDFECVYYVLSADYNRYMDVQQAINFAIHGDF
                     ESLGVQFAYPTQTLWMSGGSARA"
     gene            4895..6226
                     /locus_tag="PL152_06850"
     CDS             4895..6226
                     /locus_tag="PL152_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HQR49036.1"
                     /translation="MKPPHPAGRFLATREWRRRLVFWGGAVATGAVAVAFALGSDFAT
                     SLFRQACSAWQWWPFLATPLGLALCVWITRTWFAGSEGSGIPQTIAALNLPSETERDR
                     LLSPRIAVGKIMVTLLALASGASIGREGPTVQVGASIMHSLRRYARFPVVDVDRGLIL
                     AGGAAGVAAAFNTPLAGVVFAIEELSRSFEERTSGVILMAVIIAGVTSMALVGDYTYF
                     GSTSAALSFPGMWLVVPLCGIGGGLFGGIFSRMMLTARRQLPGPLRRIAASRPVLFAG
                     FCGMLLAAIGWVGDGTTFGTGYLEARDLLQEGHYPAPAFALLKGLATIVSYATGIPGG
                     IFAPSLSVGAGIGGQFHAWVPIAPLGTFVVLAMAAYFAGVVQAPLTALVIVVEMTNDR
                     SLTLPLMAAALLGRGASALVCREPLYRSLAERFLPHGDRGTVPASKPLPDT"
     gene            complement(6212..6571)
                     /locus_tag="PL152_06855"
     CDS             complement(6212..6571)
                     /locus_tag="PL152_06855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006801 - superoxide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-Mn family superoxide dismutase"
                     /protein_id="HQR49037.1"
                     /translation="MTIELPPLHYPRDALAPHLSAETIDFHYGKHHQAYVMNLNGLVK
                     GTEFEHASLEDIVCATSSPVFNNAAPAWNHTFERPLPACDVREHAHYIDDRNARPSYV
                     TAFWKLVNREAVGSRVR"
     gene            complement(6661..6978)
                     /locus_tag="PL152_06860"
     CDS             complement(6661..6978)
                     /locus_tag="PL152_06860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49038.1"
                     /translation="MQKINVAALATAGALMWGFCVFLVGLANMAVPGYGSAFLNLVAS
                     IYPGYHPNGNFFVLMLGTAFALADGAIAGGILGWLYNLVAVPPRREGGMATPHHDLRH
                     PTV"
BASE COUNT         1193 a         2465 c         2440 g         1237 t
ORIGIN      
        1 gaacgattct tcgaggtgga cgagccgctc gcgttcctga tcgggcctga ctcggcgatc
       61 gaggaacacc ccctcgggct tgcgacgacg ttcctggatg cgacgcgcgc ctactgggaa
      121 gactgggtgc gcggactcgc aatcccgttc gaatggcagc aggcggtgat tcgcgccgcg
      181 atcaccctca agctctgcac ctatgaggac acgggcgcgg tgctggcggc gctgacgacc
      241 tcggttccgg agagcccgga cagtggacgc aactgggact acaggtactg ctggctgcgc
      301 gacagctacc tcgtcatcca ggcgctgaac cgccttggcg cgacccggac gatggaaggt
      361 tacctgcgct tcatcaagca cacgttcggc agcgacgaca tccgccgcct gcaaccgctg
      421 ttctcgatca cgggagagcc ggaggtcccc gaacggcagg tggccacgct ccccggctac
      481 cggggcatgg ggccggtgcg ggtcggaaac gacgcctaca agcaggacca gcacgatgtc
      541 tacggcgcac tcatactcgc gtcggcacat tccttctgcg acgagcggat cggttcgccg
      601 ggagacgtcg cgctgttcaa tcgcctggaa ccgatcggcg ccaaggccgt cgagttctgg
      661 cagggcccgg acgccggccc gtgggaattc cggggtgcgg tgcgctcgca cacgttctcc
      721 gcggccatgt gctgggccgg ctgcgaccgg ctggcgagga tcgccggcag gctggcgctc
      781 ccggcccgcg agagtcactg gcgcaacgca gccgacggga tccgcgagca ggtgctctcg
      841 cgctcctgga acccgacgcg ccgggcattc tgctcgagct tcggcgggga ccacgtcgac
      901 gcgaccgccc tcgtgctgcc ggatctcggc atcgtgccgc cgcacgatcc tcgattcctg
      961 tccaccgtcg aggcgatcga gcacgacctc aaggacgggg actggatcta ccgttaccgc
     1021 cacgaggacg atttcggagt gccggccacg gcgttcacga tctgcgcatt ctggtacgtg
     1081 aacgctctcg ccgcggtcgg tcgtcgcgag gaggctcgcg agatcttcga gcgcctgctg
     1141 gcacggcgca atcggctcgg gctgctttcg gaagacatcg acccgcgctc gggcgaactc
     1201 tggggcaact ttccgcagac ctacagcatg gtgggcatca tcaactccgc ggtacgcctc
     1261 agtcgctcgt gggaagaggc gatgtgatgg tggcgtgaca tgaggtggag tgctgtccgc
     1321 aacatcgcca ttcggtacct cgtgccgatc gtcgccgccg tcagcctggt ggcgttcgtc
     1381 ggcgttccct acatcgacca cattctcgcc ggctggttcc gctccgacgt gcagatgcgt
     1441 gccaggctgg tcatgacctc cgtcgaagag ccactgggga acctgctcga tggagcggaa
     1501 ctggacaggg cacgaaccta cctcgggcgg gtcgccagcg acgaacgcct gctcggggtt
     1561 gtcgtctgcg caccggacgg ctcgcgggtc gtgcgcaccg tgctggttcc cgacacggtg
     1621 acctgcgcca cggatgccac tcagccgtcc gacggcagca cggcacgaac ggtcggggcc
     1681 ggcctggtcg agatatcgcg atttccgctg gtgaccgctg ccggacattc cttcgaggtg
     1741 ctgatcgttc acgacctgag cttcatcgat cgtcgccaga cgagcgcgcg caatttcgtg
     1801 ctggccttcg cgctgctcgc ggcttgtgcg ctcgccgtgc tcgccggcct gggtgtccgc
     1861 ctgctgctca accagtggat ccggctgctc gtcggtgaca tcagcagcaa gcgcttcctg
     1921 gacgatgccg agtcgtcccg ggcgtcggtg cccgtcctga tggaggtacg ccaggcgctg
     1981 cgcgatctgg aagccagtca gcgcctcgag atcgagttcc aggagaactg gacgccgcag
     2041 gcgctgcagc aggtcgtgcg agagagtctc gagtccagcc cgatgctcgt ggtgtccaac
     2101 cgggagccgt acatccacaa cctcgacgcg cagggacaac cggtcgtcca ggtgcccgcc
     2161 agtggcatgg tcaccgcaat cgagccggtc atgcgcgcgt gttccggtac gtggatcgcg
     2221 cacggcagcg gcacggccga ccgtctcgtc gtcgacaggc gcgaccacgt gcgcgtcccg
     2281 cccgacgacc cgagctacga attgcgccgc gtctggctga cgcagcccga ggaagaaggc
     2341 tactactacg gcttctccaa cgagggtctc tggccgctct gccacctggc gtacgtacgc
     2401 cccgccttcc gcgaagccga ctggaggcag tacatcgagg tcaatggccg tttcgccgat
     2461 gtcgtggccc aggaggcccg gaagaccgag cccgtggtgc tgatacagga ttttcacttc
     2521 gccctgctgc cccgcctgat tcgcgagcgg ggcatccgcg ccacgatcgc gctgttctgg
     2581 cacatcccgt ggcccaacgc cgagacgttc ggtgtctgcc cctggaagcg cgagatcctg
     2641 ctccacatgc tgagcgcgga cattctcggc ttccacaccc gttaccactg ccagaacttc
     2701 cttgcgaccg tcgatcgctt catcgagtgc cagatcgacc aggaacacat gacggtgacg
     2761 caccaggggc acgtgtgccg cgtcaaggcg tacccgatct cgatcgagtg gccgccgcgg
     2821 tggctggccg gtgccccgag tcccgagaat gcgaggacga acgtgaagcg acggtggggc
     2881 atcggcccgg acgtccggat cggcgtcggc gtcgaacgat gggatttcac caagggaatc
     2941 gtcgagcgct tccagtcgtt cgagacgctg ttcgagaagc aaccggaatg gaagggccgc
     3001 ctgacgttcc tgcaggttgc cgcgccgtca cgcagcaagc tgcccgccta tcaggcactg
     3061 cagcagcaga cgctggatga actccatcga atcaatgccc gcttcggcac cccggactgg
     3121 cagccgatca tgctgatcgg ccaacaccag gagcccgccc aggtcttcga gctttaccga
     3181 gccgccgact tctgcctggt caacagcctg catgacggga tgaacctggt cgcgaaggaa
     3241 tttgtcgcgg ctcgcgacga cgaggacggg gtgctggtgc tgagctcctt taccggcgcc
     3301 tcccgcgaac tggtcgaggc cctgctggtg aacccgttcg acgtgtctga aacggcacgg
     3361 gcgatcgtga cggccctcga gatgccccgc gaggagcggc gcaagcgcat gcagctcatg
     3421 aggcggacca tctccgaggc gaacgtgtac cgctgggcgg gccgcatgct catggacgtc
     3481 gcccgcatcc ggcagcgcca gacgctcacc cggtagacaa ccgctgcctg acgccgtgcg
     3541 tcccggtaac accggacgac cccggtcgcg gccggtgcgt gatcgatttt cgccgctcgg
     3601 cagcgagtcc gtgcgcgcac gcagctaggc gcgcgcgctg ccgccggaca tccagagtgt
     3661 ctgggtcggg taggcgaact gcacgccgag ggactcgaag tcgccgtgga tcgcgaaatt
     3721 gatcgcctgc tgcacgtcca tgtagcgatt gtagtcggcg ctcaacacgt agtagacgca
     3781 ctcgaagtcg agcgaatagg ggccgaattt cgcgaagtgc gcgcggtcga agcgaactcc
     3841 gtcccggcgc tcgatcgccg agcgcagcac cgtcgcgacc ttctccagca acgcacgcgg
     3901 cgtttcgtag gtcaccccca ccgtgaacac gacacgccgc tcccgcatgc ggccgtaatt
     3961 gcgcaagcga ccgcccagca ggtcggcgtt cgacatgacg atctgctcgc cgctgagcga
     4021 gcgcagccgc gtgctcttga tgccgacgta ctcgaccgtg cccatgagat cgccgacgac
     4081 caggaagtcg ccgatgacga acggctggtc gagcgcgatc gacagcgaag cgaacaggtc
     4141 tcccagcaca ttctgcacgg caagtgcgac ggcgaccccg ccaatgccga gccctgcgac
     4201 cagcgccgtg atgtcgaatc cgaggttgtc gagggtgagc agcacgacca gcgcccagac
     4261 gaccatgcgc gcgaggaacc ggatgatgcc gatggtgcct gcagcggcct tgtccgactc
     4321 cagcgcgacc tttcgccgtt ggtccagcca ggcaccgacc gcggtcgtcg cccagacgcc
     4381 ggactgccag aacagcgcga cggcaaaggt cctcgccagc agcgactccg catgtgcgcc
     4441 gaagtcaccc agcgcgaggc cgccgcaaag cgacgcgacc gccatgaaga tccatgtggt
     4501 gcggctcgcg acctgcagcg ggatctcgag cagctccgtc tgcccggtgc gctcgaagcg
     4561 gcggtagtgt cgtcgcgcca cgctgcgcaa ggcccacagg accgcgagca gcacggcgcc
     4621 aacggccgcc gccaggacca ggtctgttcc atgcgaactc agccaggccg atggcacctt
     4681 gggaaaccat tcgcccaacg ggcagcactc ctcgctccgg cgcgacaggc cggctctcgt
     4741 cgccactcta agcgcgcaag gggcgcgtca gtaatgacat ttcctcatgg caatcgtgag
     4801 gacatttcag cggaccgggc cggctcgcca aagcagtcgg gcgggcccgg tgggttgccc
     4861 gtcagcgcgt gcatctgtgg cagagtgagc gatcatgaag cctccacacc cggccggccg
     4921 tttcctggcg acgcgcgagt ggcggcggcg cctcgtcttc tggggcggcg cggtcgccac
     4981 cggcgcggtg gcggtcgcct tcgcgctggg cagcgatttc gctacgagcc tgttccgcca
     5041 ggcttgctcg gcatggcagt ggtggccgtt cctggcgacg ccactcgggc tcgcgctttg
     5101 cgtgtggatc acgcggacgt ggttcgcggg ctccgagggc agtggcattc cacagacgat
     5161 cgccgcgctc aatctgccaa gcgagaccga gcgcgaccgc ctcctgagcc cgcgaatcgc
     5221 ggtaggcaag atcatggtca cgctgctggc gctggcgagc ggagcgtcga tcggtcgcga
     5281 aggcccgacg gtgcaggtgg gcgcctcgat catgcactcc ctgcggcgat atgcgcggtt
     5341 cccggtggtg gacgtggacc gcgggctgat cctcgcaggc ggcgcagcgg gcgtcgccgc
     5401 agccttcaac acgcctctcg ccggcgtcgt gttcgcaata gaggagctgt cgcgctcgtt
     5461 cgaggagcgc acgagcggcg tgatcctcat ggcagtcatc attgccggtg tcacctcgat
     5521 ggcgctggtc ggtgactaca cgtacttcgg tagcacgtcg gcggccctct cctttcccgg
     5581 gatgtggctg gtcgtgccgc tgtgtggcat cggcggtggt ctgttcggcg ggatcttctc
     5641 gcgcatgatg ctgacagcac gccggcagct gccgggtccg ctgcgccgaa ttgcggcgtc
     5701 gcgccccgta ctgttcgccg ggttctgcgg aatgctgctt gcggcgatcg gctgggtcgg
     5761 cgacggcacg accttcggca cgggatatct cgaagcccgc gacctgctgc aggagggcca
     5821 ctacccggcg cccgctttcg ccctgctgaa aggcctcgcg acgatcgtgt cctacgcgac
     5881 cggaatcccc ggcggaatct tcgctccgtc actttcggta ggcgccggca tcggcggcca
     5941 gttccatgcc tgggtgccga tcgcaccgct gggcaccttc gtggtgctcg ccatggccgc
     6001 gtatttcgcc ggcgtcgtgc aggcaccgct cacggcgctc gtgatcgtcg tcgaaatgac
     6061 gaacgaccgg tcgctcacgc tgccgctcat ggccgccgcc ctgctcggcc gcggcgccag
     6121 cgcgctcgtc tgccgcgaac cgctgtaccg atcgcttgcg gagcgattcc tgccgcacgg
     6181 cgatcgcggg acagtgcccg cctcgaagcc tctaccggac acgtgacccg acagcctccc
     6241 ggttgacgag cttccagaat gcggtgacgt agctcgggcg tgcgttgcga tcgtcgatgt
     6301 agtgcgcgtg ctccctcacg tcgcacgcgg gcagcgggcg ctcgaaagtg tggttccagg
     6361 ctggtgccgc attgttgaac acggggctcg acgtcgcgca cacgatgtcc tcgagcgacg
     6421 catgctcgaa ctcggttccc ttgacgaggc cgttcaggtt catgacgtac gcctgatggt
     6481 gcttaccgta gtgaaagtcg atggtctcgg cggagagatg cggcgccagc gcgtcgcgcg
     6541 ggtaatggag gggcggcaat tcgatcgtca tgggatacct ccttgcggtg atgccgggct
     6601 ggcctccgtc cacgcgccaa acgtggtctc gccaccagcc tctccgcttc gcagtccggt
     6661 tcaaaccgtc gggtgcctca agtcgtggtg cggcgttgcc ataccaccct cccgacgcgg
     6721 cggtacggcg acgaggttgt acagccagcc gaggatgccg ccggcgatcg cgccgtcggc
     6781 cagcgcgaag gcggtaccga gcatcagcac gaagaaattg ccgttcgggt gatagccggg
     6841 gtaaatggac gcgaccaagt tcaggaaagc ggacccatag ccaggtacgg ccatgttggc
     6901 tagcccaacc aggaaaacgc agaagcccca cataagggca cccgcggtcg cgagcgctgc
     6961 cacgttgatc ttctgcatgt ctacctcctg tgcgctgggc gccatgagat cggccggaaa
     7021 tcgggcttct gacggtggga ctgcccggcg gcggcgattg ttccctaagc cgtcggcgac
     7081 cgccggatcc tggccaccag actgcgtccc cgactgccat ttcggcacct actgattctc
     7141 gtatgctgca cgcgagttaa tttgtcgcgc agcggcatcg cgcgattcgg ccagatgcag
     7201 ccccgcggaa tccctggaga cacgatatga ccacgctcgc gccgcaacgc atgacgccgt
     7261 ccgaggcact cgtcgaaacc ctcgtcgcga acggcgtgac cgacgtgttc ggcatcgtgg
     7321 gctcggcgta catgg
//
LOCUS       DAOMFD010000096         7225 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3555_length_7225_cov_34.852720, whole genome shotgun sequence.
ACCESSION   DAOMFD010000096 DAOMFD010000000
VERSION     DAOMFD010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7225)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7225
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3555_length_7225_cov_34.852720"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1128
                     /locus_tag="PL152_06865"
     CDS             <1..>1128
                     /locus_tag="PL152_06865"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002882.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HQR49039.1"
                     /translation="LHVDMDAFYASVEQRDDPSLRGRPVIVGGTGARGVVAAASYEVR
                     KFGVHSAMPMREALRRCPDAVCVHPRISHYAAESARIFEVFHEFTPLVQGLSLDEAFL
                     DVTASIALKGEPAHIASEVKRLIRERTELTASVGVAPNKLVAKIASDLRKPDGLVVVL
                     PGQVEEALDPLPIRRLFGLGAKTAPKVEALGIHTLRDLRLASPSRLRPIFGRYTERVQ
                     QRAAGIDDRPVVPDPDEKQISAEETFDADIGDHGRLRAEIVRLADKTCARLRARELAA
                     NCVTVKIRRGDFTTYTRQRHIEPATQETRVVTAVATELLDQWLAGQPRAALRLLGVGV
                     SELGPAAQLDLFTAPQTARNRELDATVDRIREKFGKLALKPA"
     assembly_gap    1129..1228
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1229..1665)
                     /locus_tag="PL152_06870"
     CDS             complement(<1229..1665)
                     /locus_tag="PL152_06870"
                     /inference="COORDINATES: protein motif:HMM:NF015999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HQR49040.1"
                     /translation="MAKQARVSETPAVAFLKANRVAFTEHPYEYLEHGGAQHSAEVLG
                     LDPYAVVKTLVMQDAAGTPLIVLMHGNRTVSTKNLARQIGVKSVEPCAPEVANRHSGY
                     LVGGTSPFATRRKMPVFVERSILELPRIWINGGRRGYLVGIEPA"
     gene            1847..3496
                     /locus_tag="PL152_06875"
     CDS             1847..3496
                     /locus_tag="PL152_06875"
                     /inference="COORDINATES: protein motif:HMM:NF012234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR49041.1"
                     /translation="MYTSFFGLQEKPFAITPDPRYLYLSARHAEALAHLLYGINEAGG
                     FIQLTGEVGTGKTTVIRSLLEQLPGHADVALILNPRVTPAEFLLTICEELHIPVPESG
                     RNSTKTLMDLLGRHLLDTHARGRRVVLIVDEAQNLSTDTLEQVRLLTNLETATTKLLQ
                     IILIGQPELRDLLDRAELRQLAQRITGRYHLDPLSQDESAGYVRHRMRVAGATGEVFT
                     VSALREIHRLSHGVPRVINVVSDRTLLGAFTREEHRAGGALVRAAASEVYGRPIPAPW
                     LKWATAGAVVAALALVTVGAVSLWSARRATPEPVAAQPHAATAAAAPAPAAPAPQATP
                     SHAPAQPPLQELLVGHASETTTEGALGQLFALWGATYDPAKGRGCDQAAAQGLDCLFQ
                     KGSWAQLRTLNRPAILTLTDDTGRTHQVVLAGLTDDTATLRLGGEPRKVPIAEASRYW
                     FGDFLLLWRPPMAVAKSLGPGMRGDDVRWLRESLRAAQGLPAAPGGDAYDADLTRLVQ
                     DFQRQHRLAVDGVAGVQTQIVLDTVIRATGSPTIVNPSAGG"
     gene            3500..4357
                     /locus_tag="PL152_06880"
     CDS             3500..4357
                     /locus_tag="PL152_06880"
                     /inference="COORDINATES: protein motif:HMM:NF027851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspB"
                     /protein_id="HQR49042.1"
                     /translation="MSFILDALRKSEHDRQRQTGPTLAEVPVAAPKPRSNVWAPVVIV
                     LLLVNLVVIGVLLVRKAAREPAAAQVAAAPAATSTTAGQPPAAAPAPVAAAAPAIAAP
                     AVGASTAPAITTLPPPASSPAAQPATAVAAADARPPVNPLEAEASGAPPMLDPTMAER
                     AAAAPPGPPAVMRTPTTRGSVVYESLPDSSTVGGPPPAPQPATNRQAATTGAGLPSAD
                     ELAAAGVPALNLDLHVFSTVPGERLIFVNSHKYREGDTMQEGPVVRQITPAGAVLEFN
                     GRDYLLSNK"
     gene            4354..5490
                     /locus_tag="PL152_06885"
     CDS             4354..5490
                     /locus_tag="PL152_06885"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR49043.1"
                     /translation="MTGSAKIETLAVHAGREPDAATGALREPIHLSTTFERSADGSYP
                     HGHYYTRASNPNRDSLERALAALEGGAGAVAFASGSAATLAAFSLASPGGRIVCSSDC
                     YHGTGKQLRDLLPGFGLSAEFVDTSDTAAVARALGAPTSLVWVETPSNPLLKVSDLAE
                     LARLAHEHGALFACDNTFASPVLQRPFDHGADLVMHSTTKYLGGHSDVLGGVLVVREQ
                     GAVLERLRDYQGTTGGVPSPFDCWLVLRSLATLPLRVRAQATSAMAIARYLHGDIRVE
                     RVHYPGLEDHPAHSLAQRQMRDGFGAVVSFQVPGGAAEAMAVAARVKLFTRATSLGGI
                     ESLVEHRASMEGPHGSTPPNLLRMSIGLENVADLIADLDQALGT"
     gene            complement(5497..5907)
                     /locus_tag="PL152_06890"
     CDS             complement(5497..5907)
                     /locus_tag="PL152_06890"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR49044.1"
                     /translation="MVRKALIGLVGVLAAWCALADAPQVAQVSQQALLDMQAQGTPML
                     LLDVRTPEEFAAGHIAGAVNIPYDQVASRLEEIPKDKEVVLYCKSGRRSGMAAETLAA
                     NGYTKLGHLDGDMQGWTAAGRPVEGTAPQPAEAR"
     gene            complement(5984..6376)
                     /locus_tag="PL152_06895"
     CDS             complement(5984..6376)
                     /locus_tag="PL152_06895"
                     /inference="COORDINATES: protein motif:HMM:NF013228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQR49045.1"
                     /translation="MNERRNISTGTPWEPVVGYSRAVRVGSMVWVAGTTATGPDGRIV
                     APGDAAAQTRQALENVRAALARAGAKMEHVVRTRIFVRDIRGWEAIGRAHGEYFGAIR
                     PATAMVEVSGLVDPDMLVEIEVDACITE"
     gene            complement(6411..6833)
                     /locus_tag="PL152_06900"
     CDS             complement(6411..6833)
                     /locus_tag="PL152_06900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR49046.1"
                     /translation="MPKAGNLAVEPAVADDVPVFGPRPYAEALMARVGGETVGFALYF
                     HNYSTFAGRPGVYVEDLYIRPQHRGRGYGEAMLHHLAALAVARGCARFEWSVLDWNEN
                     AIAFYRKLGAVAMDDWTVYRVTGDALARLAAGEPRPAA"
     gene            6939..>7225
                     /locus_tag="PL152_06905"
     CDS             6939..>7225
                     /locus_tag="PL152_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR49047.1"
                     /translation="MAAESSRLPLNALRVFESVATHLSFTAAAKELGVTTAAVSAQVK
                     ALEADIGVPLFRRHTRAVALTQEGAELLPGVRRGLDEMRRAVDRIRRERVGG"
BASE COUNT         1061 a         2614 c         2400 g         1050 t
ORIGIN      
        1 cctgcacgtc gacatggacg cgttctacgc gtcggtggag cagcgcgacg acccgtcgct
       61 gcgcgggagg cccgtgatcg tcggcgggac gggggcacgc ggcgtcgtgg ccgcggcgag
      121 ctacgaggtg cgcaagttcg gcgtgcactc cgccatgccc atgcgggagg cgctgcgccg
      181 atgcccggat gcggtgtgcg tccacccgcg gatctcgcac tacgcggcgg agtcggcccg
      241 gatcttcgag gtcttccacg agttcacgcc gctggtgcag gggctttcgc tcgacgaggc
      301 gttcctcgac gtgactgcca gcatcgcctt gaagggagaa cctgcacaca ttgcgtccga
      361 agtgaaacgc ctgatccgcg agcggacgga gctgaccgcg tcagtcggcg tggcgccgaa
      421 caagctggtc gccaagatcg cctccgacct gcgcaagccc gacggcctcg tcgtcgtgct
      481 ccccgggcag gtcgaggagg ccctcgaccc cctgcccatc cgtcgcctgt tcgggctcgg
      541 cgccaagacc gcgcccaagg tcgaggccct cggcatccac accttgcgcg acctgcggct
      601 cgccagtccg tcgcgactgc gtccgatctt cggccgctac accgagcgcg ttcagcagcg
      661 tgcggccggg atcgacgacc ggccggtcgt gccggacccc gacgagaagc agatcagcgc
      721 ggaggagacg ttcgacgcgg acatcgggga ccacgggcgc ctgcgtgccg agatcgtgcg
      781 cctcgccgac aagacctgcg cgcgcctgcg ggcccgcgaa ctcgcggcga actgcgtgac
      841 cgtgaagatc cgccgtggcg acttcacgac ctacacgcgg cagcggcaca tcgagccggc
      901 gacgcaggag acccgcgtcg tcaccgccgt ggcgaccgag ttgctggacc agtggctcgc
      961 gggccagccc cgcgccgcgc tccgtctgct cggcgtcggc gtgagcgagc tcggcccggc
     1021 cgcgcagctc gacctgttca cggccccgca gaccgcgcgc aaccgggaac tcgacgcgac
     1081 cgtcgaccgg attcgcgaga agttcggcaa gctggcgttg aagccagcnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1201 nnnnnnnnnn nnnnnnnnnn nnnnnnnngc cggctcgatg cccacgaggt atccgcgccg
     1261 tccgccgttg atccagatcc tgggcaactc gaggatgctg cgctcgacga acaccggcat
     1321 tttcctccgc gtggcaaacg gcgaggtgcc gccgacgaga tagccactgt ggcggttcgc
     1381 cacttccggg gcgcaaggct ccaccgactt cacgccgatc tggcgcgcaa gattcttggt
     1441 cgagacggtc cggttaccgt gcatcaacac gatgagtggc gtgcccgccg catcctgcat
     1501 tacgagcgtc ttgaccaccg cgtaggggtc gagcccgagc acttcggcgc tgtgctgggc
     1561 gccgccgtgc tcgaggtact cgtacgggtg ctcggtaaac gcgactcggt tggccttgag
     1621 gaaggccacg gcaggggttt cactgacgcg tgcttgcttg gccatcggct cggcccggtg
     1681 gtgcggcggt ccgcgcggaa ttctaccggt acccgcgcca ccgccggctc gcgcgggcca
     1741 gcgcaggccc gttccggtgg ccggggtggc gcaggctggc catgcccggt ccgcgcgggt
     1801 atcgttggcg gcccaaccca cccgcccgcg ccacagctaa ccccgcatgt acacgagctt
     1861 tttcgggctc caggaaaagc cgttcgcgat cacgccggac ccgcgctacc tgtacctgag
     1921 cgcgcggcac gcggaggcgc tcgcgcacct gctctacggc atcaacgagg cgggcggatt
     1981 catccagctg acgggtgagg tcggcaccgg caagacgacc gtgatccgca gcctgctcga
     2041 gcagctgccc ggccatgcgg acgtggccct gatcctgaac ccgcgcgtca cgccggcgga
     2101 gttcctgctc accatctgcg aggagctgca catcccggtg ccggaatcgg gacgcaacag
     2161 caccaagacg ctcatggacc tgctcggccg ccacctgctc gacacgcatg ctcgcggccg
     2221 gcgcgtcgtg ctgatcgtcg acgaggcgca gaacctgagc accgacacgc tcgagcaggt
     2281 gcgcctgctc accaacctcg agactgccac gaccaagctg ctgcagatca tcctgatcgg
     2341 ccagccggag ctgcgtgacc tgctcgaccg ggcggaactc cggcagctcg cgcagcgcat
     2401 caccggccgc tatcacctcg atcccctgtc gcaggacgag tccgccggct acgtccgtca
     2461 ccgcatgcgg gtcgccggcg cgaccggcga agtgttcacc gtgtcggcgc tgcgcgagat
     2521 ccaccgcctg agccacggcg tgccgcgcgt gatcaacgtc gtctccgatc gcacgctgct
     2581 cggagccttc acgcgcgagg aacatcgcgc aggcggggcg ctggtgcgcg cggcggcctc
     2641 cgaagtctac ggccgtccca ttcctgcacc ctggctcaag tgggccacgg ccggcgcagt
     2701 cgtcgcggcg ctcgcgctcg tgaccgtcgg ggcggtgagc ctgtggtccg ctcggcgcgc
     2761 cacgccggaa ccggtcgccg cgcaaccgca cgccgcgacg gcagcggcgg cacctgcccc
     2821 ggctgcaccg gcaccccagg ccactccgtc gcatgccccc gcgcaaccac cactgcagga
     2881 actgctcgtt ggccatgcca gcgagacgac caccgagggc gcgctcggcc agctcttcgc
     2941 gttgtggggc gcgacctacg atccggccaa ggggcgcggc tgcgaccagg ccgcggccca
     3001 gggactcgat tgcctgttcc agaagggctc gtgggcgcag ctgcgcaccc tcaatcgtcc
     3061 cgcgatcctg acgctcacgg acgacacggg ccgcacccac caggtcgtgc tcgccggcct
     3121 cacggacgac accgccacgc tgcggctcgg cggagagccg cgcaaagtgc cgatcgcgga
     3181 ggcgtcgcgc tactggttcg gcgatttcct gctgctttgg cgaccgccga tggccgtcgc
     3241 gaaatccctc ggacccggaa tgcggggcga cgacgtgcgc tggctgcgcg agagcctgcg
     3301 cgccgcgcag ggactgccag cggcacctgg cggcgacgcg tatgacgcgg acctcacgcg
     3361 gctggtgcag gacttccagc gccagcaccg gctcgccgtc gacggcgtcg cgggcgtaca
     3421 gacccagatc gtgctcgata ccgtgatccg tgcgaccggc tcgcccacga tcgtcaatcc
     3481 gtcggcgggt ggctgagcga tgtcgttcat cctcgacgcc ctgcgcaagt ccgagcacga
     3541 tcgccagcga cagaccggac cgactcttgc cgaggtgccg gtcgcggcgc cgaagccacg
     3601 cagcaacgtg tgggcgccgg tcgtcatcgt cctgctgctc gtgaacctcg tggtgatcgg
     3661 cgtgctgctc gtgcgcaagg cagcgcgcga gccggcggca gcgcaggttg cagccgctcc
     3721 ggctgcgacg tccacgaccg cgggacaacc gcctgccgcc gcaccggcac cggtggcagc
     3781 ggctgcgccc gcgattgccg cgccagcggt gggcgcctcg accgcgcctg ccatcaccac
     3841 tctgccgccg cctgcatcat cgcccgctgc acagcctgcg actgcggtag ccgccgccga
     3901 tgcccggccg ccggtcaacc cgctcgaggc ggaagcgagc ggcgcccccc cgatgctcga
     3961 tccgacgatg gccgaacggg cggccgcggc cccgccggga ccgccggccg tgatgcggac
     4021 gccgaccacg cgcgggtcgg tcgtctacga atcgctgccc gactcctcga cggtcggtgg
     4081 ccctccgcca gccccgcaac cggccacgaa ccggcaggcg gcgacgaccg gcgccggact
     4141 gccctcggcc gatgaactcg ccgcggccgg cgtgcctgcg ctcaacctcg acctgcacgt
     4201 gttctcgacg gtgccgggcg agcggttgat cttcgtgaac tcgcacaagt accgcgaggg
     4261 agacaccatg caggaaggcc cggtcgtccg gcagatcacg ccggccggcg cggtcctcga
     4321 attcaacggc cgcgactacc tgttgagcaa caagtgaccg gcagcgcgaa gatcgagacc
     4381 ctggcggtgc acgccggccg cgaaccggac gcggcgaccg gcgctctgcg cgagccgatc
     4441 cacctgtcca cgacgttcga gcggagcgcc gatggaagct atccgcacgg ccactactac
     4501 acgcgcgcga gcaacccgaa ccgcgattcg ctcgagcgcg cgctcgcggc gctagaaggc
     4561 ggcgccgggg ctgtcgcatt cgcctccggg tccgccgcga ccctcgcggc cttttcgctc
     4621 gcgtcgcccg gcgggcggat cgtgtgctcg tcggactgct accacggcac cggcaagcag
     4681 ctgcgcgacc tgctcccggg cttcgggctc agcgcggaat tcgtcgacac gtcggacacg
     4741 gcggccgtcg cccgggcgct cggggcgccg acatcgctcg tctgggtcga gacgccatcg
     4801 aatccactgc tcaaggtgag cgatctcgcg gagctggccc ggctcgccca cgagcacggc
     4861 gcgctgttcg cgtgtgacaa cactttcgcg agtcccgtcc tgcagcggcc gttcgaccac
     4921 ggcgcggacc tcgtcatgca cagcacgacc aagtacctcg gcgggcacag tgacgtgctc
     4981 ggcggcgtgc tcgtagtgcg cgagcagggt gccgtgctcg agcgattgcg cgactaccag
     5041 ggaacgaccg ggggcgtccc gtcgccgttc gattgctggc tggtgctgag gagtctcgcg
     5101 actctaccgt tgcgggtccg ggcgcaggcg accagcgcga tggcgatcgc acgctatctg
     5161 cacggcgata tccgggtcga gcgtgtccat tacccgggcc tggaagacca tccggcgcac
     5221 tcacttgcgc agcggcagat gcgcgacggc ttcggcgcag tcgtgtcctt ccaggtgccc
     5281 ggcggcgccg cggaggcgat ggcggtcgcc gcgcgcgtga aactcttcac gcgcgccacc
     5341 agcctcggcg ggatcgagag cctcgtcgag caccgcgcct cgatggaagg accgcacggc
     5401 agcacgcccc cgaacctgct gcgcatgtcg atcgggctcg agaacgtggc cgacctgatc
     5461 gccgacctgg accaggcgct gggcacctga gccgggctac ctggcctcgg cgggctgcgg
     5521 cgcggttccc tcgacgggtc gtccggcagc cgtccagccc tgcatgtcac cgtcgaggtg
     5581 cccaagcttg gtgtagccgt tggcggccag cgtctcggct gccatgcccg agcggcgtcc
     5641 cgacttgcag tacaggacca cttccttgtc cttcgggatt tcctcgaggc ggctcgcgac
     5701 ctggtcgtac gggatgttca ccgcaccggc gatgtgcccg gctgcgaact cctcgggagt
     5761 gcgcacgtcg agcaggagca tcggcgtgcc ctgcgcctgc atgtccagca gggcctgctg
     5821 ggaaacctgc gcgacctgcg gcgcatccgc cagcgcgcac cacgctgcca gcactcccac
     5881 caagccgatc aacgcttttc gcaccattgc cgtctccata ttgacgccgt tcataccgat
     5941 tgggccgccg acgaatcttt gaccccctcc cagcggcact cgttcactcc gtgatgcagg
     6001 cgtcgacctc gatctcgacg agcatgtccg ggtcgacgag gccgctcact tcgaccatcg
     6061 ccgtggccgg gcgaatggca ccgaagtact ccccgtgcgc ccgcccgatc gcctcccagc
     6121 cgcgaatgtc gcgcacgaag atccgcgtcc gcacgacgtg ttccatcttc gcgcccgcac
     6181 gagccagcgc ggcgcgcacg ttctccagcg cttgccgggt ctgagcggcc gcgtcgcccg
     6241 gagccacgat gcgtccatcc ggtcccgtcg cggtcgtgcc ggcgacccac accatggagc
     6301 ccacgcggac ggcccgcgaa tagccgacga ccggttccca gggtgtgccg gtcgagatgt
     6361 tccgacgctc gttcatgtgc ctgcctcctg ttgcacgctg gaccggctgc tcaggcagcg
     6421 ggtcgcggct cgcctgctgc caggcgcgcc agcgcgtcac ccgtcacacg atagaccgtc
     6481 cagtcatcca tcgcgacggc cccgagcttg cggtagaagg caattgcgtt ctcgttccag
     6541 tcgaggaccg accactcgaa ccgggcgcaa ccgcgcgcga ccgccaacgc agcgaggtgg
     6601 tgcagcatcg cctcgccgta accgcgaccg cgatgctgcg gacgtatgta caggtcctct
     6661 acatagacgc cggggcgccc cgcgaacgtc gagtagttgt gaaagtagag tgcgaagccg
     6721 acggtctcgc cgccgactcg ggccatgagc gcctcggcat acggccgcgg tccgaatacc
     6781 ggcacgtcgt cggcgaccgc cggctcgaca gccaggttcc cagccttcgg catggcgcct
     6841 ccttttttca gtttccctcg aaagacaacg gcggaatgcc ttgaagtcct cgggattcgc
     6901 cagtattgtc aaccaatagt ttactcgagg tcgggccgat ggcagcggaa tccagccgtc
     6961 ttccactgaa cgcgctgcgc gtcttcgagt ccgtggccac gcacctcagt ttcaccgctg
     7021 ccgcgaagga gctgggcgtt acgacggccg cggtcagcgc gcaggtgaag gcactcgaag
     7081 ccgacatcgg cgtcccgctc ttccggcggc acacgcgcgc cgtggcgctg acgcaggaag
     7141 gcgccgagct gctgcccggc gtgcgtcgcg gcctcgacga gatgcgccgc gccgtggacc
     7201 gcatccggcg cgagcgggtc ggcgg
//
LOCUS       DAOMFD010000097         6984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3793_length_6984_cov_38.632270, whole genome shotgun sequence.
ACCESSION   DAOMFD010000097 DAOMFD010000000
VERSION     DAOMFD010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6984
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3793_length_6984_cov_38.632270"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1127)
                     /locus_tag="PL152_06910"
     CDS             complement(<1..1127)
                     /locus_tag="PL152_06910"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR49048.1"
                     /translation="MLWNTVLLALRAIRRNLLRSFLTVLGIVIGVAAVITMVTLGNGA
                     TRSVTEQISSMGSNLLIAIPGQRFGPGGDNAPRFKIADADALRSQVSAARLVAPVVNA
                     SAKAVYQANNWTTVVTGSTDDYFESGNWQLKSGRTFNEAEERAGRAVCVIGETVRQKL
                     FGARDPVGEEIRVRQFACEVIGLLKSKGQSAMGSDQDDVIVMPLRTVQRRLTGNQDVG
                     RLMVAVAPGASIDAVKEQVTGLLRERRKIADDEDDDFRVMDTRQLVQTLTSTTKILTM
                     LLAAVAAVSLLVGGIGIMNIMLVSVTERTREIGTRLAIGALEREVLLQFLVEAIVLAG
                     LGGLAGIALATGASLVLAKLMGVPYIFSPGINLLSFGFATA"
     gene            complement(1127..1831)
                     /locus_tag="PL152_06915"
     CDS             complement(1127..1831)
                     /locus_tag="PL152_06915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR49049.1"
                     /translation="MSDAPMIRLRAVSRTYGQGQAAVHALNGIDLDVEQGEFVAIMGP
                     SGSGKSTAMNILGCLDVPSSGSYEFQGVRVEQLSREQRALLRRNFLGFVFQGFNLLAR
                     TTALENVELPLLYRGEPAGRRHAAARDALRQVGLAGREDHTPAELSGGQQQRVAIARA
                     IVTRPAVLLADEPTGNLDTHTSAEIMDLVTRLNATQGITVLMVTHEADVAAYARRIVR
                     FVDGRIESDRPRVEAA"
     gene            complement(1828..3108)
                     /locus_tag="PL152_06920"
     CDS             complement(1828..3108)
                     /locus_tag="PL152_06920"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR49050.1"
                     /translation="MNDALPPGPPRHGLQDLLARSGDHRNRRWLVAGAVVAVAAIALV
                     LVRSGGNGDAPTYRTEAAAIGDLVVKVSATGNLQPTNEVQVGSELSGIIETVYVDDND
                     RVKKGQVLARLDVSKLEDAIEKSRANLAAAEAQVAQSDATVTESRATLERYREVSRLS
                     DGKVPSRSEMDTAEANLKRAEASAASARASVAQARANLRSDETNLEKASIRSPIDGIV
                     LKRDIEPGQTVAASFQAPVLFTLAEDLRKMELQVDVDEADVGRVKEGQPATFTVDAWP
                     GRRYDAKITRVGYGSKETDGVISYLTVLAVDNDDLSLRPGMTGTAEITTLTRDHVLLV
                     PNAALRFNPTNADAQPQRQRGIANLLMPRPPAPVRRAPVQRGETPHVWILKDDAPERV
                     DVKTGATDGKLTEITGGGLTAGSQVITEAAVAAP"
     gene            complement(3105..4526)
                     /locus_tag="PL152_06925"
     CDS             complement(3105..4526)
                     /locus_tag="PL152_06925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HQR49051.1"
                     /translation="MIAPICLAGCVAVGPDYEPPATGAPQAWTSLQPQGQPALTPDQA
                     GHLAAWWSELDDPLLGECVETALRANPDVASAQARLRQVRAQRVVAASGLYPSLGASG
                     NARRTNASAESSGRDATIDNYGAALDASWEIDVFGGTRRGVEAATADVQASAASLAAT
                     QVSLAAEVASNYVDVRLQQALIRIARDNAESQFETLQLTQWRAQAGLASVQDVEQART
                     NLERTRALVPALETSLAQSEHRLDTLLALTPGTLHARLAAHAPPLVVPRRIAVGIPAD
                     TLRQRPDVQAAERNLAAETARVGQAEAALYPSLNLTGSIGVEALSLGNLGSGDAGAWS
                     LLAGITAPIFEGGRLRAQVEAQDAVREQALAAYRKTVLTALEEVENALVALSKNHERE
                     LSLNTAVDSARTADTLARERYSAGLIDFQSVLDTDRTVLAVEESLAQNRADGVQALVG
                     LYRSLGGGWSPDTAAAPAGKASP"
     gene            4904..6187
                     /locus_tag="PL152_06930"
     CDS             4904..6187
                     /locus_tag="PL152_06930"
                     /inference="COORDINATES: protein motif:HMM:NF025702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4397 domain-containing protein"
                     /protein_id="HQR49052.1"
                     /translation="MRMRILLRCAVLAGALLVLASCNSGGNGGKASVRVVNASMDYAS
                     VDLYLDDKRKLAAVGFETVSGFAKADAGDYTVEFRQADAAGALESFSKKFGTGDRWSY
                     VVLGSSGHFQVLAIDETHKEADTGKAFIRLVNAADDAGALDIYLTDESTALTDASPIF
                     AGVPADTLADEGLVVLDKGSYRLRVTAAGSTSDLRLDRSGVALASKGVYTLVVCGTPG
                     GVLADAMLVPQRGEATVIPTDKARVRAVVGLGGGTSVSVTVDDTALIQTAAIPVLGDY
                     QLVGAGTAAVALAVDGTDVPVGNVQLAAGSDYSLVYTGAGSAVTQSVVADVNRLPASG
                     AFKIRLVNAMSALDEPLSMSVDFVPVLDAVGLGSAALSDAIDAVDNGRLDVTRDTTLS
                     VLYSATSLTLNSRAVYTQVMFGSAAAPAGTLRKDR"
     gene            complement(6241..6834)
                     /locus_tag="PL152_06935"
     CDS             complement(6241..6834)
                     /locus_tag="PL152_06935"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQR49053.1"
                     /translation="MGQGRTLSRDYKHVKRQTPGGQAFSGWVGLLAGLAIGLTIALGV
                     FLHYRDLAPPEPHASVAKAPASSQAAEDPAAPPADPEKDFTFYNMLPKQEVEVPAPGA
                     KGTGKPAAPGLPAGDVVLQAGSFKQPAEAEKLQARLAQYGVDAKIQRFALEDETWYRV
                     RVGPIATVQELETIRAKLAEAEVEATPVTPEFQEPPP"
     gene            complement(6835..>6984)
                     /locus_tag="PL152_06940"
     CDS             complement(6835..>6984)
                     /locus_tag="PL152_06940"
                     /inference="COORDINATES: protein motif:HMM:NF017555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HQR49054.1"
                     /translation="NDFHTYYNAHQFLVDDAELREARLALVQGLRQVVRNGLGLLGVS
                     APEAM"
BASE COUNT          996 a         2380 c         2466 g         1142 t
ORIGIN      
        1 atcgcggtgg cgaagccgaa tgacagcagg ttgatgcccg ggctgaagat atagggcacg
       61 cccatcagtt tcgcgagcac gagggacgcg ccggtcgcga gcgcgattcc ggcgagtccg
      121 ccgaggcccg ccagcacgat ggcctcgacc aggaactgca gcagcacctc gcgctcgagg
      181 gcgccgatcg cgagccgagt gccgatctcg cgggtccgct cggtgaccga gaccagcatg
      241 atgttcatga tgccgattcc gcccaccagc aggctcaccg cagcaacggc ggcgagcagc
      301 atggtgagga tcttcgtcgt gctggtgagc gtctggacca gttgccgcgt gtccatgacc
      361 cggaagtcgt cgtcctcgtc gtcggcgatc ttgcgccgtt cccgcagcag gcccgtgacc
      421 tgctccttga ccgcgtcgat cgacgcaccg ggtgccacgg ccaccatgag ccggcccacg
      481 tcctggttgc cggtgaggcg tcgctgcacc gtgcgcagcg gcatcacgat gacgtcgtcc
      541 tggtccgagc ccatggcgga ctggcccttg gacttgagca gcccgatcac ctcgcaggcg
      601 aactggcgca cccggatctc ctcgcccacc gggtcgcggg cgccgaagag cttctggcgc
      661 acggtctcgc cgatgacgca cacggcccgc ccggcacgct cctccgcctc gttgaacgtg
      721 cggccggact tcagttgcca gttgcccgac tcgaagtagt cgtcggtcga gccggtcacc
      781 accgtcgtcc agttgttcgc ctggtagacg gccttcgccg acgcattgac gacgggcgcg
      841 acgagcctcg cggccgacac ctggctgcgc aacgcatcgg catcggcgat cttgaatcgt
      901 ggcgcgttgt cgccgcccgg cccgaagcgc tggcccggaa tggcgatcag caggttgctg
      961 cccatggagg agatctgctc ggtcaccgag cgggtggctc cgttgccgag cgtcaccatg
     1021 gtgatcacgg cggccacgcc gatcacgatg ccgagcacgg tgaggaacga gcgcaacagg
     1081 ttgcggcgga tcgcgcgcag cgccagcagc acggtgttcc acagcatcag gcggcctcca
     1141 cgcgcggccg gtcgctctcg atgcggccgt ccacgaagcg cacgatgcgg cgagcgtagg
     1201 cggcgacgtc cgcctcgtgc gtgaccatca gcacggtgat gccctgcgtc gcattgaggc
     1261 gcgtgacgag gtccatgatc tcggcgctgg tgtgggtgtc caggttgccc gtgggctcgt
     1321 cggcgagcag gacggcgggg cgcgtcacga tggcccgggc gatggcgacg cgctgctgct
     1381 ggccacccga gagttccgcc ggcgtgtgat cctcgcgccc tgcgagccct acctgccgca
     1441 gggcgtcgcg cgcggcggca tggcgacgac cggccggctc accgcggtac agcagcggca
     1501 gttcgacgtt ctcgagcgcc gtggtacggg cgagcaggtt gaacccctgg aacacgaacc
     1561 cgaggaaatt gcgtcgcaac agcgcgcgct gctcgcgcga cagctgctcg acgcgcacgc
     1621 cctggaattc gtagctcccg ctgctgggca cgtcgaggca gccgaggatg ttcatcgcgg
     1681 tggacttgcc ggagccactc ggccccatga tggcgacgaa ttcgccctgc tccacgtcga
     1741 ggtcgatccc gttgagcgcg tgtacggcgg cctggccctg gccatacgtc cgcgacacgg
     1801 cgcgcaggcg gatcatcggc gcgtcgctca tggcgccgcg acggctgcct ccgtgatgac
     1861 ctgcgagccg gccgtgagcc cgccgccggt gatttccgtg agcttgccgt ccgtggcgcc
     1921 ggtcttgacg tcgacgcgct cgggggcgtc gtccttgaga atccacacgt gcggcgtctc
     1981 gccgcgctgg acgggcgcgc ggcgcaccgg cgccggcggc cgtggcatca gcagattggc
     2041 gatgccgcgc tgtcgctgcg gctgcgcgtc cgcattcgtc gggttgaacc ggagcgccgc
     2101 gttcggcacc agcagcacgt ggtcgcgcgt gagcgtggtg atttcagccg tgcccgtcat
     2161 gccgggtcgc aggctcaggt cgtcgttgtc gaccgcgagc accgtgaggt acgagatgac
     2221 gccgtcggtt tccttggagc cgtagcccac ccgcgtgatc ttcgcgtcgt acctgcggcc
     2281 cggccatgca tccacggtga acgtggcagg ctggccttcc ttgacgcgcc cgacgtccgc
     2341 ctcgtcgacg tcgacctgca gttccatctt gcgcaggtcc tccgccagcg tgaacagcac
     2401 cggcgcctgg aacgatgccg cgacggtctg tcccggctcg atgtcgcgct tgagcacgat
     2461 gccgtcgatc ggcgagcgga tgctggcctt ttcgaggttg gtctcgtccg aacgcaggtt
     2521 cgccctggcc tgtgccacgc tggcgcgcgc gctggcggcg ctggcctcgg cgcgcttcag
     2581 gttggcctcc gccgtgtcca tctcgctgcg cgacgggacc ttgccgtccg acaggcggga
     2641 gacttcacgg tagcgctcca gcgtggcccg gctttcggtg acggtcgcat ccgactgcgc
     2701 gacctgtgcc tcggcggcgg cgagattcgc gcgcgatttc tcgatcgcgt cctcgagctt
     2761 cgagacgtcg aggcgcgcga gcacctgccc cttcttcacc cggtcgttgt cgtcgacgta
     2821 caccgtctcg atgatgccgg agagttcact gccgacctgc acctcgttgg tcggctggag
     2881 gttgccggtg gcggagacct tcacgacgag gtcgccgatc gccgcggcct cggtgcgata
     2941 ggtcggcgcg tcaccgttgc cgcccgatcg aaccagcacc agcgcgatcg cggcaaccgc
     3001 gaccaccgcg cctgcgacga gccagcgccg gttgcggtgg tcgccggagc gggcgagcaa
     3061 gtcctgcagg ccatggcgtg gggggccggg cggcaatgcg tcattcatgg cgaggccttc
     3121 cctgcgggcg cggcggcggt gtcaggagac cagccgccgc ccagtgacct gtagagtccg
     3181 acgagtgcct gcactccgtc ggcgcgattc tgtgcgaggc tctcctcgac cgcgagcacc
     3241 gtgcggtcgg tgtcgagcac cgactggaag tcgatgaggc cggcgctgta acgctcccgt
     3301 gccagcgtgt cggcggtgcg cgccgagtcc accgccgtat tgagcgagag ctcgcgctcg
     3361 tgattcttcg acagcgcgac aagggcattc tcgacttcct ccagcgccgt caggacggtc
     3421 ttgcgatagg ccgcgagcgc ctgttcgcgg accgcgtcct gcgcctcgac ctgcgcccgc
     3481 aggcgaccgc cctcgaagat gggcgccgtg atgccggcga gcagcgacca cgcgcccgcg
     3541 tcaccgctgc cgaggttgcc gagcgacagc gcctcgacgc cgatcgagcc ggtgagattc
     3601 aacgatggat acaacgctgc ctcggcctgt ccgacgcgtg cggtctcggc cgcaaggttg
     3661 cgctcggcag cctgcacgtc gggtcgctgg cgcagggtgt cggcagggat gcccacggcg
     3721 atgcgccgtg gcacgacgag cggaggagcg tgcgcggcga gccgcgcgtg cagggtgccg
     3781 ggcgtgagag cgagcagcgt gtcgaggcgg tgctcggact gcgcgaggct cgtctcgagc
     3841 gccggaacca gcgcgcgggt gcgttcgagg ttggtgcgcg cctgttcgac gtcctggacg
     3901 ctggcgagcc ccgcctgtgc gcgccattgc gtgagctgca gcgtctcgaa ctggctttcc
     3961 gcattgtctc gggcgatgcg gatcagcgcc tgctgcagcc gcacgtcgac gtaattgctc
     4021 gcgacctcgg cggcgagcga tacctgggtc gccgccaggc tcgcggcgct cgcctgcacg
     4081 tcggcggtgg cggcctccac gccgcggcgg gtaccgccga acacgtcgat ctcccagctc
     4141 gcatcgagcg cggctccgta gttgtcgatc gtcgcgtctc gaccggaact ctccgcgctg
     4201 gcattggtgc gccgggcgtt gccgctcgca cccagtgacg ggtagagacc ggaggcggcg
     4261 acgacgcgtt gcgcgcgcac ctggcgcagc ctggcctggg cgctcgcgac atccgggttc
     4321 gcccgcaacg cggtctcgac gcactcaccg agcagggggt catcgagttc gctccaccat
     4381 gcggcaaggt gcccagcctg gtcgggcgtc agcgccggct gcccctgcgg ttgcagcgat
     4441 gtccacgcct gtggagcgcc agtcgccggc ggctcgtagt ccgggccgac ggccacgcag
     4501 ccggccaggc aaatcggtgc gatcaaggcg aggcgccgga caatcatgcg cgacgcaccc
     4561 cgcgcattct cgaatgcggg tgcgacggta cggacctggg caggcgtgtc agtggggtgc
     4621 cgtgcatagg gcccgaattg tcgcgcgtac cgtgcccgct ggacaaaaaa catcgcaccc
     4681 tgcccgggaa aatcccgcaa ttgttcgact gtgagcgcgg gggcgagttc cgcctcgacc
     4741 cctgccaagg acttgtcggg gcgggaggtg ctgtgcgaaa gcccccgagg cccggcagtc
     4801 gcagcgacat tcgtcatccg gcctgtggat ttcccgaact tgccggtgtt ccgtggtcaa
     4861 tcgcatgctt gcccaggcgg gcaaaggaat caggagtctg gccatgcgca tgcgaatcct
     4921 tctccggtgc gcggtcctgg ccggcgcgct gctcgtgctg gcgagctgca attcgggggg
     4981 caacggcggc aaggcgtcgg tccgcgtcgt gaacgccagc atggactacg cttcggtcga
     5041 tctctacctc gacgacaagc gcaagctcgc ggcagtcgga ttcgagactg tcagcggctt
     5101 cgcgaaggcg gacgcgggtg actacaccgt ggagttccgc caggccgatg ctgctggcgc
     5161 gctcgagtct ttcagcaaga aattcggcac cggcgatcgc tggagctatg tcgtgctcgg
     5221 gtcgagtgga cacttccagg tattggcgat cgacgagacg cacaaggagg cggacacggg
     5281 caaggcattc atccgactcg tcaacgcggc ggacgacgca ggggcgttgg acatttacct
     5341 gaccgacgag tcgacggcac tgacggacgc ttcgccgatc tttgccggcg tcccggccga
     5401 tacgctggct gacgagggcc tcgtggtgct cgacaagggc agctacaggc tgcgggtgac
     5461 ggccgcgggc agcacttcgg acctgcggct cgaccggtcc ggagtggcgc tcgccagcaa
     5521 gggcgtgtac acgctggtcg tgtgtggcac gccgggagga gtcctggccg acgccatgct
     5581 ggtgccgcag cgcggcgagg caacagtcat ccccaccgac aaggcacggg tgcgcgccgt
     5641 ggtcggcctc ggtggcggga ccagcgtgag cgtgaccgtc gacgacacgg cgctgatcca
     5701 gacggccgcg ataccggtgc tcggtgacta tcagctggtg ggcgcgggca cggcagcggt
     5761 ggccctggcc gtggacggga ccgatgtccc ggtgggcaac gtgcagctcg ctgccggctc
     5821 cgactatagc ctcgtctaca ccggcgccgg ctcggcagtc acccagtcgg tcgtcgccga
     5881 cgtgaaccgt ctgcccgcat ccggcgcgtt caagatccgg ctcgtcaacg ccatgagcgc
     5941 actcgacgag ccgctctcga tgagcgtgga cttcgtgccg gttctcgacg cggtcggcct
     6001 cgggtcggcc gcgctgtcgg acgcgatcga cgcggtggac aatgggcgac tcgacgtcac
     6061 gcgcgacacg acgctgtcgg tgctgtactc ggccacgtcg ctgacgctga acagccgcgc
     6121 cgtttacacg caagtgatgt tcggctccgc ggcagcaccc gctggcacgc tgcgcaagga
     6181 ccgctgacga aatcggggac attctccaat ttcgcttggt gcgaaattgg agaatgtccc
     6241 ctacggggga ggctcctgga actccggcgt gacgggcgtc gcctcgacct cggcctcggc
     6301 gagcttggcg cggatcgtct cgagttcctg caccgtcgcg atcggcccga cgcgcacccg
     6361 gtaccacgtc tcgtcctcga gcgcgaagcg ctggatcttc gcgtccacgc cgtactgcgc
     6421 aaggcgcgcc tggagcttct cggcctccgc gggctgcttg aacgatccgg cctgcagcac
     6481 cacgtcgccg gcaggcaggc cgggcgcggc cggcttgccc gtgcccttgg cgccgggtgc
     6541 gggcacctcc acttcctgct tggggagcat gttgtagaag gtgaagtcct tctcgggatc
     6601 cgcaggaggt gccgccgggt cctcggcagc ctgcgatgac gcaggcgcct ttgcgaccga
     6661 agcgtgcggc tcgggcggcg cgagatcgcg gtaatgcagg aataccccga gcgcgatcgt
     6721 cagaccgatg gcgagccccg cgagcaggcc gacccacccg ctgaatgcct gcccgcccgg
     6781 agtctgccgc ttgacgtgct tgtagtcgcg gctgagcgtc cgcccctggc ccacttacat
     6841 cgcctccggc gccgacacgc cgagcagccc gagcccgttg cgcacgactt gccgcagccc
     6901 ctggacgagc gccagccgcg cctcgcgcag ttcggcgtcg tccaccagga actggtgcgc
     6961 gttgtagtag gtgtggaagt cgtt
//
LOCUS       DAOMFD010000098         6923 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3862_length_6923_cov_39.429674, whole genome shotgun sequence.
ACCESSION   DAOMFD010000098 DAOMFD010000000
VERSION     DAOMFD010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6923)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3862_length_6923_cov_39.429674"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /gene="modA"
                     /locus_tag="PL152_06945"
     CDS             complement(<1..797)
                     /gene="modA"
                     /locus_tag="PL152_06945"
                     /inference="COORDINATES: protein motif:HMM:NF024921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_function: GO:0030973 - molybdate ion binding [Evidence
                     IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HQR49055.1"
                     /translation="MSNERERRYFRLGVCTAVLLLAVLPAAGAEDAASRGPEIGVAVA
                     ANFAGTAEQLCRAWFAQRPGRCVITPGASGVLASQAAQGAPFDIFLSADRQRPERLEV
                     DGVAVPGTRFTYAIGRLVLWVPQWRGGADLRAALADAGVRIVAIAKPTAAPYGAAAIE
                     TLHSLGLDRDDSRRVVQGENVAQAFQFVASGAADAGFVAASQVLDYEARTGKQLAGAA
                     IAVDPALHAPIEQQAVLLARARANADARALLEFLKSAEAQRLIESAG"
     gene            980..1597
                     /locus_tag="PL152_06950"
     CDS             980..1597
                     /locus_tag="PL152_06950"
                     /inference="COORDINATES: protein motif:HMM:NF024879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper domain-containing protein"
                     /protein_id="HQR49056.1"
                     /translation="MNAMNRNSRTQGLRRLALALAWSAIVLPSVSLASPPPWAPAHGW
                     RAKNDPSYPGYSGRNWDHDYGVRSGRCDREEVGALLGGIAGGTLGAAAGKDANRAVAI
                     AVGTVVGAAIGAEIGRRMDRTDRACTGHSLELAATGRSVTWRNVRTGVTYQLTPIEAG
                     PSGTGCRKFKLTATGAFGLSEGRATACPGGDAVWNLAPETRVSQR"
     gene            complement(1607..3793)
                     /gene="malQ"
                     /locus_tag="PL152_06955"
     CDS             complement(1607..3793)
                     /gene="malQ"
                     /locus_tag="PL152_06955"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HQR49057.1"
                     /translation="MSGPALAQLARCRGIGDAYHDYRGELRSFDLATKAAILAAMGCR
                     VDDAAAVQLEIERCDTRHWRGLLPGIVVARPGHMGVPLTIPAGRPELALEWRAQLEDG
                     SVRSGVAQAHGLAKVDSRDVEGRALERRILVLPEDLPPGYHTLTTTTVDDGAAACTLV
                     VAPGRCYEPDVLAQGARTWGVSVQLYALRSPSNWGIGDFADLAEVVRYCALQEAAFVG
                     LNPLHALFPADPAHCSPYSPSSRHYLNVLYVSVESLPEYRECEPARKVVGSDAFRATL
                     ARLRAAELVDYRGVATAKLPVLRLLYSHFRREHLARDTARARSFRDFQAERGESLRRH
                     ALHDAIDEHLRGENPARYRGWRTWPGTLRDPGHAAVQAFGRQHADDVEFHEWLQWIAD
                     DQLRAVQRLANELGMPIGLYGDLAVGANPAGSETWSAQSVYRTGAAIGAPPDPLALKG
                     QDWGVPPLDPQALAEGGYQSFRELVAANMRQFGALRLDHVMALFRQWWVPAGLPATDG
                     GYVHYPLEDLMSVLALESHRHRCLVIGEDLGTVPDEVRRAMAEFGVYSYKVLLFEKGA
                     DGRFRRPGDYARRAIASVTTHDLPTLRAYWEGKDLELRDQLDLFPSADTHRHVCEERE
                     RDRAALLAALANADLRPDGVAGPDSPYAPELARAVHLYLARSASALVALQVEDLIGMT
                     DPVNVPGTHEEYANWQRKLADEIADVLGSPGALGLLAEVQAARSRG"
     gene            complement(3796..5943)
                     /gene="glgX"
                     /locus_tag="PL152_06960"
     CDS             complement(3796..5943)
                     /gene="glgX"
                     /locus_tag="PL152_06960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HQR49058.1"
                     /translation="MNPIMGTPEASQHAAGLEAGSPQPLGANWTGRGINFAVFSANAT
                     RVQLCLFDASGRQETAQLDLPARTGDVWHGFLPARYGGIGTLYGYRVHGPYEPVAGHR
                     FNPAKLLVDPSATALAGDLEWHDALNGAVVGNDALPDPADSAPYVPKSRVTDPAFDWG
                     GVRSPNVPWRDTILYELHVKGYTRRHPGVPEPLRGKYLGLAEPAVIDHLKRLGVTTVE
                     LMPCQAFVSERVLAARGLGNYWGYNPLAWFAPDARYAIADPVAEFKTMVRALHDAGLE
                     VVLDVVFNHTAEGNEGGPTLSFRGYDNATYYRLFPHNRATYKNFSGCGNTVNLDNPEV
                     RALVIDCLRYWTAEMRVDGFRFDLAPVLGRDSSGYNQAAPFFAALRADPILAYVKLIA
                     EPWDVGPGGYQLGHFPPGWSEWNDRYRDTMRAFWRGDRPLVGNFAERFAGSSDLFRDR
                     GRKPTASINFITSHDGFTLHDLVSYNERHNEANLEGGADGHQHNLSWNCGAEGPTDDP
                     AIVRLRARQMRNLLTTLLLSQGVPMLLAGDEMARTQLGNNNAYCQDNEVSWLDWSLAE
                     THAALVEFVRRLVDLRRSRLWLRRDTFLKGARRTAAASDVAWLHPSGREMTQADWGDG
                     GLRCLAVQMSGGTQSRAGAAGDLLVVFNADDASVPLPLPRASRGDAWTVLVDTSDPGI
                     VAPRILRVGETLVVESRSTILLESGPVPAEGAS"
     gene            complement(5940..>6923)
                     /locus_tag="PL152_06965"
     CDS             complement(5940..>6923)
                     /locus_tag="PL152_06965"
                     /inference="COORDINATES: protein motif:HMM:NF014822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase C-terminal domain-containing
                     protein"
                     /protein_id="HQR49059.1"
                     /translation="VDAVASMLYLDYSRRPGEWVPNRFGGRENLEAIEFLKQLNVAVG
                     HYHPGAMTIAEESTAYPGVTRPVHLGGLGFHFKWNMGWMNDTLRYAAHDPVHRRFHHR
                     LVTFSFVYAWSENFVLPISHDEVVHGKGSLLDKMPGDEWQKRANYRLFRAYMTAHPGK
                     KLLFMGSEFGQWHEWRDEDSLDWPLLEDPRHRGLLDLNRDLNRMYADLSALHAGDADP
                     AGFGWIDLRNAELSVFAFLRRDPSRPQDPPIVCVFNATPVPRDEYWIGAPEGGRYTKL
                     LDTDDPRYGGSGYDSRRTLVADDRPSHGYPHCLRLRLPPLAALYLRPDRQP"
BASE COUNT         1012 a         2452 c         2450 g         1009 t
ORIGIN      
        1 tacccggccg attcgatgag gcgctgcgcc tctgccgact tgaggaactc cagcagcgcg
       61 cgcgcgtcgg cgttcgctcg cgcacgcgcg agcagcacgg cctgctgctc gatcggcgcg
      121 tgcagcgcgg gatccactgc aatcgctgcg ccggcgagct gcttgccggt gcgggcctcg
      181 taatcgagca cctgcgacgc ggccacgaaa cctgcgtccg cagcgccgct cgcgacgaac
      241 tggaacgcct gcgccacgtt ttcgccctgc acgacgcgcc ggctgtcgtc ccggtcgagc
      301 ccgagggaat gcagtgtctc gatggcggcc gccccgtacg gcgccgccgt gggcttcgcg
      361 atcgcaacga tccttacgcc ggcatccgcg agcgctgcac gcaggtcggc gcccccacgc
      421 cactgcggta cccacagcac cagccggccg atcgcgtagg tgaatcgcgt cccgggcacg
      481 gcgacgccgt cgacctcgag tcgctccggc cgttgccggt ccgcggacag gaagatgtcg
      541 aagggtgcgc cctgggcggc ctgcgacgcg agcactccgc tcgcgcccgg cgtgatcacg
      601 caacgccccg gccgctgcgc gaaccatgcg cggcacagct gctccgcagt cccggcgaag
      661 ttggcggcga ccgcgacacc gatctcgggt ccgcggcttg cggcatcctc ggcgccagcg
      721 gccggtagca cagccagcag cagcaccgcc gtgcacaccc cgagccggaa gtagcgtcgc
      781 tcacgttcgt ttgacatatc cgcatcccgc gggaagccgg ggccatctta ggggatcggc
      841 ggcgcgcgcc gaaatcgacc gaccagaggt cgctttcgtg accggtttca ccgtcccggc
      901 cgaatgccgc tccctcgccg gcggggccgc ctagggtgat cgcgtcgccg ctcgtggcgg
      961 cttgcgacga ggtacatcga tgaacgcgat gaaccgcaac tcgcgcaccc agggactgcg
     1021 ccggcttgcg ctcgccctcg cctggtcggc catcgtgctg ccctccgtgt cactggcgtc
     1081 tccaccgccg tgggctccgg cgcacggctg gcgcgccaag aacgacccgt cgtacccggg
     1141 gtacagcggt cgcaactggg accacgacta cggcgtccgg tcgggccgct gcgatcgcga
     1201 ggaagtcggc gcgctgctcg gcggcatcgc cggcggcacg ctcggcgctg ccgcgggcaa
     1261 ggacgccaac cgtgccgtcg caatcgcggt cggcacggtc gtcggggcgg ccatcggcgc
     1321 ggaaatcggc cggcgcatgg accggaccga ccgcgcgtgc acgggccact cgctggaact
     1381 ggcggcgacc gggcgcagcg tcacgtggcg caacgtgaga accggcgtga cctaccagct
     1441 gacgccgatc gaggccgggc cgtcgggcac cggatgccgc aaattcaagc tcaccgcgac
     1501 gggcgcattc gggttgagcg agggccgcgc cacggcctgc ccgggtggcg atgccgtgtg
     1561 gaacctggcg cccgagaccc gcgtctcaca gcgctgaacg gcgtcctcag ccgcgtgagc
     1621 gcgccgcctg gacttccgcg aggagtccga gcgctccggg actgccgagc acgtcggcga
     1681 tctcgtccgc gagcttgcgc tgccagttgg cgtactcctc gtgcgtgccc gggacgttca
     1741 ccgggtcggt catgccgatc aggtcctcga cctgcagtgc caccagcgcg gaggcactgc
     1801 gcgccaggta gaggtgcacg gcgcgcgcca gctccggggc atacggcgag tcgggcccgg
     1861 cgacgccgtc gggacgcagg tccgcattcg ccagtgctgc gagcaaagcc gcacgatcac
     1921 gctcgcgttc ctcgcacacg tggcggtgcg tgtccgcgct cgggaacagg tcgagctggt
     1981 cgcgcaactc gaggtccttc ccctcccagt aggcgcgcag tgtcggcagg tcgtgcgtgg
     2041 tcacgctggc tatcgcgcgc cgtgcgtagt cgccgggccg ccggaaacga ccgtccgctc
     2101 ctttctcgaa caacagcacc ttgtacgaat acacgccgaa ttcggccatc gcccgccgga
     2161 cttcgtccgg aaccgtcccg aggtcctcgc cgatgacgag gcagcggtgg cgatggcttt
     2221 cgagcgcgag caccgacatg aggtcctcga gcggatagtg cacgtagccg ccgtcggtcg
     2281 ccggcagtcc cgccgggacc caccactggc ggaacagcgc catcacgtga tcgagcctga
     2341 gcgcgccgaa ctgccgcatg ttcgccgcga ccagttcgcg gaacgactgg tagccgccct
     2401 cggccagcgc ctggggatcc agcggcggca cgccccagtc ctggcccttg agggccagcg
     2461 gatcgggcgg cgcgccgatg gccgcgcccg tgcgataaac ggactgcgca gaccaggttt
     2521 ccgaccccgc ggggttcgcg ccgaccgcga ggtcgccgta cagcccgatc ggcatgccga
     2581 gctcgttcgc gagtcgctgc acggcccgca gctgatcgtc cgcgatccac tgcagccact
     2641 cgtggaactc gacgtcgtcg gcatgctgcc gcccgaacgc ctgcaccgca gcgtggcccg
     2701 gatcgcgcag ggtcccgggc cacgtgcgcc atccgcggta gcgcgcggga ttctcgccgc
     2761 gcaggtgctc gtcgatcgcg tcgtgcaggg cgtggcgacg caacgactcg ccgcgctcgg
     2821 cctggaagtc gcggaacgac cgggcacgcg ccgtgtcccg ggccaggtgc tcgcgacgga
     2881 aatgcgagta caggaggcgc aggacgggca gcttcgccgt ggccacgccg cggtaatcga
     2941 ccagttccgc agcccgcagg cgcgcgagcg tcgcccggaa cgcgtccgat cccacgactt
     3001 tgcgcgccgg ctcgcactcg cggtactcgg gcagcgactc gaccgacacg tagagaacgt
     3061 tcaggtagtg gcgcgaggac gggctgtacg ggctgcagtg tgccgggtcc gcgggaaaca
     3121 gcgcgtgcag cggattcagg ccgacgaacg cggcctcctg caacgcgcag taacgcacga
     3181 cctcggccag gtcggcgaaa tccccgatgc cccagttgga aggcgatcgc agcgcgtaga
     3241 gctgcacgga cacgccccac gtacgcgccc cctgcgcaag cacgtccggt tcgtagcacc
     3301 gcccgggtgc gacgacgagc gtgcatgcgg ccgcgccgtc gtcgacggtc gtcgtcgtga
     3361 gcgtgtgata tccgggcggc aggtcctcgg gcaggaccag gatgcgccgc tcgagggcgc
     3421 gcccctcgac gtcgcgggaa tccaccttgg ccagtccatg agcctgcgcg actccgctgc
     3481 gcaccgatcc gtcctcgagc tgtgcccgcc actccagcgc cagctcgggc cgtccggccg
     3541 gaatcgtgag cggcacgccc atgtgaccgg ggcgggcgac gacgatgccg ggcagcaagc
     3601 cgcgccagtg cctcgtgtcg cagcgctcga tctcgagctg gacggccgcc gcatcgtcca
     3661 cgcgacagcc catcgccgcg aggatcgcgg ccttggtcgc gaggtcgaac gacctcagct
     3721 cgccgcgata gtcgtggtac gcgtcgccga tgccgcggca gcgcgcgagc tgtgcaagtg
     3781 ccgggccgga catcgtcagg acgccccttc tgcggggacc ggtcccgact cgagcaggat
     3841 ggtgctgcgc gactcgacca ccagggtctc gccgacgcgc aggattcgcg gcgcgacgat
     3901 gccggggtcg ctcgtgtcga cgagcaccgt ccaggcgtcg cctcgcgaag cgcggggcag
     3961 cggcaaaggc acgctcgcgt cgtcggcgtt gaacacgacc agcaggtcgc cggccgcgcc
     4021 cgcccgcgac tgcgtaccgc cgctcatctg caccgcgagg cacctgagcc cgccgtcccc
     4081 ccagtcggcc tgggtcattt cgcggcccga cgggtgcagc cacgcgacgt cgctcgcggc
     4141 ggccgtgcgg cgcgcgccct tcaggaacgt gtcacgcctg agccacagcc gggagcgcct
     4201 caggtcgacg aggcgcctca cgaactcgac gagcgcggca tgggtctccg ccagcgacca
     4261 gtcgagccac gagacttcgt tgtcctggca gtacgcgttg ttgttgccga gctgggtccg
     4321 cgccatctcg tcgccggcga gcagcatcgg cacgccctgc gacagcagca gcgtggtgag
     4381 caggttgcgc atctgccgcg cccgcagccg cacgatggcg gggtcatcgg tcggcccttc
     4441 cgcgccgcag ttccagctca ggttgtgctg gtggccgtcg gcgccgccct cgaggttcgc
     4501 ctcgttgtgc cgttcgttgt acgacacgag gtcgtgcagg gtgaagccat cgtgggaggt
     4561 gatgaagttg atgctggccg tgggcttgcg cccgcggtcg cggaacaggt cgctcgagcc
     4621 ggcgaaacgc tcggcgaagt ttccgaccag cggccggtcg ccgcgccaga acgcgcgcat
     4681 ggtgtcgcga taccggtcgt tccactccga ccagcctggc gggaagtggc cgagctggta
     4741 gccgccgggc cccacgtccc agggctccgc gatcagtttc acgtaggcga ggatcgggtc
     4801 ggcacgcagc gccgcgaaga acggcgccgc ctggttgtag ccgctgctgt cgcggccgag
     4861 caccggtgcg aggtcgaagc ggaatccgtc cacgcgcatc tcggccgtcc agtagcgcag
     4921 gcagtcgatc accagcgcgc gcacctccgg gttgtcgaga ttgacggtgt tgccgcagcc
     4981 ggagaaattc ttgtaggtcg cgcggttgtg cggaaacagc cggtagtagg tggcgttgtc
     5041 gtagccgcgg aacgagagcg tcgggccgcc ttcgttgccc tcggcggtgt ggttgaacac
     5101 cacgtcgagc acgacttcga gtcccgcgtc gtgcagcgcc cgcaccatgg tcttgaactc
     5161 ggccaccggg tcggcgattg cgtagcgtgc atccggggcg aaccacgcca gcgggttgta
     5221 gccccagtag ttgccgaggc cgcgcgctgc cagcacgcgc tccgacacga acgcctggca
     5281 cggcatcagc tcgaccgtcg tgacgccgag ccgcttcagg tggtcgatca cggccggctc
     5341 ggcgaggccg agatacttgc cgcgcagcgg ctccggcacc cccgggtgcc ggcgcgtgta
     5401 acccttcacg tgcagctcgt agaggatcgt gtcgcgccag ggcacgtttg gcgagcgcac
     5461 gccgccccag tcgaacgcag ggtcggtgac ccgggacttg ggcacgtacg gagcactgtc
     5521 ggccgggtcc ggcagtgcgt cgttgccgac tacggcgccg ttcagcgcat cgtgccattc
     5581 gaggtcgccc gcgagcgccg tggcgctcgg atcgacgagc agcttggccg gattgaagcg
     5641 atggcctgcg accggttcgt aggggccgtg cacccggtaa ccgtacagcg tgccgattcc
     5701 gccgtagcgc gcaggcagga acccgtgcca cacgtcgccg gtgcgcgccg gcaggtccag
     5761 ctgcgcggtt tcctgccgcc cgctcgcatc gaacaggcac agctgcactc gcgtggcatt
     5821 cgccgagaac acggcgaagt tgatgccgcg cccggtccag ttggcgccga gtggctgggg
     5881 cgagccagct tccaggccgg cggcgtgttg cgatgcctcg ggcgtaccca tgatcgggtt
     5941 catggctggc ggtccgggcg gaggtagagc gcggccagcg gcggcagccg cagccgcagg
     6001 cagtgcgggt agccgtggct cggtcgatcg tccgcgacca gcgtgcgcct cgagtcgtag
     6061 cccgagccgc cgtaacgcgg gtcgtcggtg tcgagcagct tcgtgtagcg gccgccctcg
     6121 ggcgcgccga tccagtactc gtcgcgcgga acgggcgtcg cgttgaacac gcacacgatc
     6181 ggcgggtcct gcggtcgcga cgggtcgcgt cgcaggaacg cgaacacgct cagctcggca
     6241 ttgcgcaggt cgatccaccc gaagccggcc gggtccgcgt cgccggcgtg cagcgccgag
     6301 aggtccgcgt acatccggtt gaggtcacgg ttgaggtcca gcagtccgcg atgacgcggg
     6361 tcctcgagca gcggccagtc gagggaatcc tcgtcgcgcc actcgtgcca ctggccgaac
     6421 tcgctgccca tgaacagcag cttcttgccc gggtgcgcgg tcatgtaggc gcggaacagg
     6481 cggtagttgg cgcgcttctg ccactcgtcg cccggcatct tgtcgagcag cgagcccttg
     6541 ccgtgcacga cctcgtcgtg cgagatcggc agcacgaaat tctcggacca cgcgtacacg
     6601 aacgagaacg tcacgagccg gtggtggaac cgccggtgca ccgggtcgtg cgccgcgtag
     6661 cgcagcgtgt cgttcatcca gcccatgttc cacttgaagt ggaagccgag gccgccgagg
     6721 tgcacgggcc gcgtcacgcc cggataggcg gtggattcct ccgcgatcgt catggcgccc
     6781 ggatggtaat gcccgaccgc gacgttcaac tgcttcagga actcgatcgc ctcgaggttc
     6841 tcgcggccgc cgaaccggtt cggcacccat tcgccggggc gccggctgta gtcgaggtag
     6901 agcatggacg cgaccgcgtc cac
//
LOCUS       DAOMFD010000099         6899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3884_length_6899_cov_35.872151, whole genome shotgun sequence.
ACCESSION   DAOMFD010000099 DAOMFD010000000
VERSION     DAOMFD010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6899
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3884_length_6899_cov_35.872151"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..564)
                     /locus_tag="PL152_06970"
     CDS             complement(<1..564)
                     /locus_tag="PL152_06970"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="E3 binding domain-containing protein"
                     /protein_id="HQR49060.1"
                     /translation="MQGEFHMPALGADMEEGTVVQWNVAPGDAVKRGQVVAVVETDKG
                     AIDVEIFEDGVVREIVVQPGTRVPVGTLLMRLNTAGGATEPSAGVPAATPAAAAEPRD
                     RASAAVATGAPEAVARTEAEARVKISPAARARSRELGVDVAIVKGTGPGGAITIEDVE
                     KAKAAGAVPNERSSMRDAIAAAMSKSKR"
     gene            complement(554..1546)
                     /locus_tag="PL152_06975"
     CDS             complement(554..1546)
                     /locus_tag="PL152_06975"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex E1 component
                     subunit beta"
                     /protein_id="HQR49061.1"
                     /translation="MTQGTRMTYREAVKQAIRAALAADPRVFLMGEDVGKYGGCFAVT
                     KGLLDEFGEERIRDTPLSESAFVGAGIGAAMGGMRPIVEVMTVNFSLLALDQIVNNAA
                     TVCHMSGGQFPVPLVIRMATGAGRQLAAQHSHSLEAWYAHVPGLRVVAPATIDDARGM
                     LRPALECPDPVIVFEPISLYNMEGELTEAAASADIEHARVHRPGRDVTLVTYGASLWR
                     CVDAADELARSRIEAEIVDLRSLRPLDDATILGSVRRTRRVVIVDEGWRTGSIAAEVA
                     MRIVEQGFYDLDAPIGRVCSEEVPIPYPKHLEDAALPGPERIVAAVRRTMGDAG"
     gene            complement(1543..2532)
                     /gene="pdhA"
                     /locus_tag="PL152_06980"
     CDS             complement(1543..2532)
                     /gene="pdhA"
                     /locus_tag="PL152_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR03182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HQR49062.1"
                     /translation="MTDMPTGVTRDRGLELLAVMLRIRQFEARCAELYGAGKIRGFLH
                     LYDGEEAVAVGVMQALGQDDAIISTYREHGHAIARGIPLGPLMAELYGKATGCSGGRG
                     GSMHIFDAATRFYGGNAIVGGGLPLAVGLGLAEKLRKGRGVAACFFGEGAAAEGEFHE
                     SLNLAALWKLPVLFLCENNLYAMGTALARSESQTDIHAKAAGYGVRAAAVDGMDVFAV
                     ESATRDAAAHARAGSGPVLLEFRTYRFRAHSMFDAQLYRDKAEVEEWRKHGPVISLTT
                     RLKAAGLMTEDDFQRIERNADAEVQAAVDFAEASGWEPVGDLTRHVYAEVRPS"
     gene            complement(2529..4292)
                     /gene="acsA"
                     /locus_tag="PL152_06985"
     CDS             complement(2529..4292)
                     /gene="acsA"
                     /locus_tag="PL152_06985"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF003313.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HQR49063.1"
                     /translation="MEWSPIAKPPGSLRREPCLTDYEKVRREFSWPEATSLLEGLPGG
                     ALNIAHEAVDRHARGPSGERLAIRFLAQGAAPHGLTYRELAADSHRFANVLTTLGVQR
                     GECVASLMGRIPAIYATVLGTLASGAVYCPLFSAFGPEPVKARLALGSARVLVTTDLL
                     YRRKVAPIRGELSELRHVLVVRTPGAGDLPEGTQDFDALMAAAPPEFAVARTRPEDYA
                     LLHFTSGTTGRPKGVLHAHAAIVQHVATGRWVLDLQDGDVYWCTADPGWVTGISYGVI
                     APLACGVGMIVDREEFDAPRWYEILDTERVTVWYTAPTAIRMLMKAGLELARSRSYPA
                     LRHLVSVGEPLNPEAVVWGVEAFGLPFHDTWWQTETGAIMIANFPCMEVRPGSMGRPV
                     PGVEAGIVRPREDGGADVVTDPDAVGELALRPGWPSMFRGYLNEEERYRKCFRDGWYL
                     SGDLAKRDADGYYWFVGRADDVIKAAGHLISPFEVESVLMTHPAVSQAGVIGVPDPVA
                     GSAVKAFVELKVGQSASEALRKDILAHARRLLGVAVAPREIAFADQLPRTRSGKIMRR
                     LLRARELGLPEGDLSTLEGAA"
     gene            complement(4398..4751)
                     /locus_tag="PL152_06990"
     CDS             complement(4398..4751)
                     /locus_tag="PL152_06990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HQR49064.1"
                     /translation="MSKSSITTRRVGNPDWVYNPHAGDMLLEEFMKPLGLSQNALARA
                     LKVSPRRINEIVHGKRAVTADTDLRLCRYFGLHEGFFLGLQNAYELLEHKRRLGDRLE
                     EIIPRSRRKSARRAA"
     gene            complement(4723..4827)
                     /locus_tag="PL152_06995"
     CDS             complement(4723..4827)
                     /locus_tag="PL152_06995"
                     /inference="COORDINATES: protein motif:HMM:NF016875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HQR49065.1"
                     /translation="MAGRHSIRINDQWRICFRWTERGAEDVEIVDYHP"
     gene            5215..6111
                     /locus_tag="PL152_07000"
     CDS             5215..6111
                     /locus_tag="PL152_07000"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HQR49066.1"
                     /translation="MNTSQMPRTDEIERRTLSGYIALAIGLLLAAAGIYRVVHPTGAG
                     PEDVLTGVPLAILGAFVLSGLYILQPNTAALLLLFGKYKGTDRTAGLRWANPFYRKHK
                     ISLRARNLASDKLKVNDKRGNPIEIASAIVWRVRDTAQATFDVENYEQYVRIQAEAAV
                     RHLASSYAYDEGEDLHEGEITLRGGQDRVAHSLIQELTDRFDLAGVVVEDAKLTHLAY
                     APEIAQVMLRRQQAEAIIAARSKIVHGAVSMVEMALNGLSERGIVHLDDERRATMVSN
                     LLVVLCSESEAQPVINAGTLHP"
     gene            complement(6142..>6899)
                     /locus_tag="PL152_07005"
     CDS             complement(6142..>6899)
                     /locus_tag="PL152_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016393102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49067.1"
                     /translation="GIALIVCITEGIPVNDMVKVKAALRDSKSRLVGPNCPGVITPDE
                     CKIGIMPGFIHKKGSVGIVSRSGTLTYEAVFQTTNIGLGQSTCVGIGGDPVRGMNFID
                     VLALFEQDPRTEGIIMVGEIGGSDEEAAAAYIRRSVSKPVVAYIAGVTAPAGKRMGHA
                     GAVISGGKGTAADKFAALEAARVTTVRSPADLGTAIAKRMRGTHAKAATRATAKPKAK
                     PKAKAKGSPKAKAVRRGKAAPARSARKTPARKK"
BASE COUNT         1047 a         2462 c         2323 g         1067 t
ORIGIN      
        1 gcgcttggac ttcgacatcg ccgcggcgat cgcgtcgcgc atgctgctgc gctcgttcgg
       61 cactgcgccg gcagccttcg ccttctcgac gtcctcgatg gtgatcgcgc cgcccggacc
      121 ggtgcccttc acgatcgcca cgtccacgcc gagttcgcgt gaccgagccc gtgccgcggg
      181 agagatcttc acgcgtgctt ccgcttccgt cctggcgacc gcttcgggcg cgcccgttgc
      241 taccgcagcc gatgcgcgat cccgaggctc cgccgccgca gcgggtgtcg ccgccggcac
      301 gcctgccgaa ggctcggtag cgccgccggc cgtattgagc cgcatcagga gcgtgcccac
      361 gggcacccgg gtcccgggct gcacgacgat ctcgcgcacc acgccgtcct cgaagatctc
      421 gacgtcgatg gcgcccttgt cggtctccac caccgccacg acctggccgc gcttgaccgc
      481 atcgccgggc gcgacgttcc actgcacgac cgtgccctct tccatgtccg ccccgagggc
      541 cggcatgtgg aattcaccct gcatcgccca tcgtcctccg cacggctgcg acgatccgct
      601 ccgggcccgg cagcgccgcg tcctcgaggt gcttcgggta cgggatcggc acctcctcgc
      661 tgcacacccg gccgatcggc gcgtcgagat cgtagaaccc ctgctcgacg atgcgcatcg
      721 ccacttcggc cgcgatactg ccggtgcgcc acccctcgtc cacgatcacg acgcggcggg
      781 tcctgcgcac ggaaccgagg atggtcgcgt cgtcgagcgg ccggagcgag cgcaggtcca
      841 cgatctccgc ctcgatccgg ctgcgcgcga gctcgtcggc ggcatcgacg cagcgccaca
      901 ggctcgcgcc gtacgtcacg agcgtcacgt cccgacccgg ccggtgcacg cgagcgtgct
      961 cgatgtccgc gctcgccgcc gcctcggtga gctcgccctc catgttgtag agcgagatcg
     1021 gctcgaagac gatcaccggg tccgggcact cgagggccgg acgcaacatg ccacgcgcgt
     1081 cgtcgatggt cgccggcgcg acgacgcgca gccccggcac gtgcgcatac cacgcctcga
     1141 ggctgtgcga gtgctgcgcg gcgagctgcc ggccggcgcc cgtcgccatg cggatcacga
     1201 gcggcacggg gaactgtccg cccgacatgt ggcagacggt cgcggcgttg ttgacgatct
     1261 ggtccagcgc cagcaggctg aagttgacgg tcatcacctc gacgatcggg cgcatgccgc
     1321 ccatcgccgc gccgatgccc gcgcccacga atgccgattc cgacagcggc gtgtcgcgga
     1381 tgcgttcctc gccgaactcg tcgagcaggc ccttggtgac cgcgaagcag ccaccgtact
     1441 tgccgacgtc ctcgcccatc aggaacacgc gcgggtcggc cgcgagtgcg gcgcggatcg
     1501 cctgcttcac ggcctcgcga taggtcatgc gcgtgccctg cgtcatgacg gcctcacctc
     1561 cgcgtacacg tgccgcgtga ggtccccgac cggttcccac ccggatgcct cggcgaaatc
     1621 gaccgccgct tggacctcag catcggcatt gcgctcgatg cgctggaagt cgtcctcggt
     1681 catcaggccc gcggccttga gtcgcgtggt cagcgagatg accggaccgt gcttgcgcca
     1741 ctcctccacc tcggccttgt cgcggtagag ctgggcgtcg aacatcgagt gcgcgcgaaa
     1801 ccgatacgtg cggaactcga ggagcaccgg gccggagccc gcgcgggcgt gcgcggcggc
     1861 gtcacgcgtg gccgattcga ccgcgaacac gtccatgccg tccaccgccg ccgccctcac
     1921 gccgtagccg gcggccttgg cgtggatgtc ggtctgcgac tcggatcgag cgagcgccgt
     1981 gcccatcgcg tagaggttgt tctcgcacag gaacagcacc ggcagcttcc acagcgcggc
     2041 gagattcagc gactcgtgga actcgccctc ggcggccgcg ccttcgccga agaagcaggc
     2101 cgcgacgccg cggcccttgc gcagtttctc ggcgagcccc aggccgaccg cgagcggcag
     2161 accgccgccg acgatggcgt ttccgccgta gaagcgcgtg gccgcatcga agatgtgcat
     2221 cgagccgccg cgcccgccgc tgcaaccggt ggccttgcca tagagttcgg ccatcagcgg
     2281 acccagtggg atgccgcgcg cgatcgcgtg gccgtgctcg cgataggtcg agatgatcgc
     2341 gtcgtcctgg ccgagcgcct gcatgacgcc gacggcgacc gcttcctcgc cgtcgtagag
     2401 gtgcaggaac ccgcggatct tcccggctcc gtagagctcg gcgcagcgtg cctcgaactg
     2461 gcggatgcgc agcatgacgg cgagcagctc gagtccgcga tcgcgggtca ctccagtggg
     2521 catatcggtc atgccgctcc ctccagcgtg gacaggtcgc cttcgggcag gccgagttcc
     2581 cgggcccgca acaggcgccg catgatcttg ccgctgcgcg tgcgcggcaa ctgatccgcg
     2641 aacgcgatct cgcgtggagc cacggccacg ccgagcagtc gccgcgcgtg cgcgaggatg
     2701 tccttgcgca gcgcctcgct cgcggactgc ccgaccttga gctcgacgaa cgccttcacc
     2761 gccgagcccg ccaccgggtc gggcacgccg atcacgcccg cctgcgagac ggccgggtgg
     2821 gtcatgagca cgctctcgac ctcgaaggga ctgatcaggt gacccgccgc cttgatgacg
     2881 tcgtcggcgc ggccgacgaa ccagtagtaa ccgtcggcat cgcgcttcgc gaggtcgccc
     2941 gagaggtacc agccgtcgcg gaagcacttg cggtagcgct cctcctcgtt caggtacccg
     3001 cggaacatcg acggccagcc cggccggagc gcgagttccc ccaccgcatc cggatcggtc
     3061 acgacgtccg ctccgccatc ctcacgcggt cgcacgatgc ccgcctcgac gccgggcacc
     3121 ggccgtccca tcgacccggg ccggacctcc atgcagggga agttcgcgat catgatggcg
     3181 cccgtctcgg tctgccacca cgtgtcgtgg aacgggagcc cgaacgcctc gacgccccac
     3241 acgacggctt cgggattgag cggctcgccc acgctcacga ggtgacgcag cgccgggtag
     3301 ctgcgcgatc gcgcgagctc gagtcccgcc ttcatgagca tgcggatcgc ggtgggcgcc
     3361 gtgtaccaga cggtgacgcg ctcggtgtcc aggatctcgt accagcgcgg cgcgtcgaat
     3421 tcttcgcgat ccacgatcat cccgacgccg caggcgagcg gcgcgatgac cccgtaggag
     3481 atgcccgtga cccagcccgg atcggccgtg caccagtaga cgtcgccgtc ctgcaggtcg
     3541 agcacccagc gcccggtggc gacgtgctgg acgatcgcgg cgtgcgcgtg cagcacgcct
     3601 ttcggtctgc ccgtcgtgcc gctggtgaag tgcagcagtg cgtagtcctc cggtcgagtg
     3661 cgcgcgaccg cgaactcggg cggtgcggcg gccatcagcg cgtcgaagtc ctgcgtgccc
     3721 tcggggagat caccggcacc gggagtgcgc acgacgagca cgtgcctgag ctcgctcagc
     3781 tcgccacgga tcggcgcgac cttgcggcgg tacaggaggt ccgtggtgac caggacccgt
     3841 gcggatccga gcgcgagacg cgccttcacg ggttcgggac cgaaagcgga gaacaacgga
     3901 caatagacgg cgcctgacgc gagcgtcccc agcacggttg cgtagatcgc cggaatccgc
     3961 cccatcagcg acgcgacgca ttcgccgcgt tgcacgccca gtgtggtgag gacgttcgcg
     4021 aaccggtgcg agtcagcggc gagttcgcgg taggtcaggc catgcggggc tgcgccctgg
     4081 gccaggaagc gaatggcgag ccgctcgccg gacggaccgc gggcgtggcg gtccacggcc
     4141 tcgtgagcga tgttgagcgc gccgcccggg aggccctcga gcagcgacgt cgcctcaggc
     4201 cacgagaatt cccggcggac cttctcgtag tccgtcaggc acggctcgcg tcgcagcgat
     4261 ccggggggct tggcgatggg cgaccattcc atgcggtcac cgtcagcagt gggttcggcg
     4321 tcgacggccc aactctagga cgggcagacc gcggattcat gcggggtttc cgcgagggga
     4381 cggggcacct gacggcttca ggcggcgcga cgtgcggact tcctgcggga ccgcgggatg
     4441 atctcctcca ggcgatcgcc gaggcggcgc ttgtgctcca ggagctcata cgcattctgc
     4501 aggccaagga agaagccctc gtgcagtccg aaatatcggc acagccgcag gtctgtgtcg
     4561 gccgtgactg cgcgcttgcc gtgcacgatc tcgttgatgc gccgcggcga gactttcaac
     4621 gcccgggcca gggcgttctg gctgagtcca agcggcttca tgaactcctc gagcagcatg
     4681 tcgcccgcgt gcgggttgta gacccagtcg gggttgccga ccctacgggt ggtaatcgac
     4741 gatttcgaca tcttcggcgc ctcgctccgt ccatcgaaag cagatccgcc actggtcatt
     4801 gatccggatg ctgtgccggc cggccaggtc gccggacagg gcttcgagcc ggttacccgg
     4861 gggatgccgc agttactcca gcgaaatgga gcactccagc aggcgcaact tccgcagtgc
     4921 ggtcgcgtga atccacggcg gcagacgacg actgcgttcg ccgctccaga tcctcgccgt
     4981 ttcgcggtcc cggaagctga cgatcaatgg ctttccctcg ccttataacg cgcgacgtca
     5041 tatgacgcaa tccgtcatag ccgttcgcat cgaccaggaa cgtcggcgca cgccgacgca
     5101 cgggcctcga gcgcaaggat ccggcaccgg ctggcgagcg tcactggcaa tatcgacccg
     5161 taacaccgca aaatccgccg tgcggcgcat taccgcgacg ttcggaggca tgccatgaac
     5221 accagccaga tgccgcgcac cgacgagatc gagcggcgca cgttgagcgg ctacatcgcg
     5281 ctcgccatcg gcctgctcct ggccgccgcg ggaatctatc gcgtcgtgca ccccacgggc
     5341 gccggcccgg aagacgtgct gaccggcgtg ccgctcgcga tcctcggcgc gttcgtactg
     5401 tccggcctgt acatcctgca accgaacacg gcggccctgc tgctgctgtt cggcaagtac
     5461 aagggcacgg accgcacggc gggcctgcgc tgggcgaatc cgttctaccg caagcacaag
     5521 atcagcctgc gcgcgcgcaa cctcgcgagc gacaagctca aggtcaacga caagcgcggc
     5581 aacccgatcg agatcgcctc ggcgatcgtc tggcgtgtgc gcgacacggc gcaggccacg
     5641 ttcgacgtcg agaactacga gcagtacgtg cggatccagg cggaggcagc ggtgcggcac
     5701 ctggcctcga gctacgccta cgacgagggc gaggacctgc acgaaggcga gatcacgctg
     5761 cgcggcgggc aggaccgcgt cgcgcactcg ctcatccagg agttgaccga ccgcttcgac
     5821 ctcgccggcg tcgtggtcga ggacgcgaag ctgacgcacc tcgcctacgc cccggagatc
     5881 gcacaggtga tgctgcggcg tcagcaggcc gaggcgatca tcgccgcgcg cagcaagatc
     5941 gtgcacggcg cggtcagcat ggtggagatg gcgctcaacg ggttgagcga gcgcggcatc
     6001 gtgcacctcg acgacgagcg ccgcgcgacg atggtgagca acctgctcgt ggtgctgtgc
     6061 tcggagagcg aggcccagcc ggtgatcaac gccggcacgc tgcacccgta gacgacacgg
     6121 tgcctggcac cgacttgccg attatttctt gcgcgcggga gtcttgcggg ccgagcgggc
     6181 cggcgcagcc ttcccgcgtc gaaccgcctt ggccttggga ctgcccttgg ccttcgcctt
     6241 gggcttggcc ttgggtttgg cagtggccct cgtcgcagcc ttcgcatgcg taccgcgcat
     6301 gcgcttcgcg atcgcggtac ccaggtcggc cggcgatcgc accgtagtga cccgcgccgc
     6361 ctcgagcgcc gcgaacttgt ccgccgccgt gcccttgccg cccgagatca ccgcgccggc
     6421 gtgacccatg cgctttccag cgggcgccgt gacgccggcg atgtaggcga cgacgggctt
     6481 gctcacggac cggcggatat acgccgccgc tgcttcctcg tcgctgccgc cgatctcgcc
     6541 gaccatgatg atgccttcgg tcctcgggtc ctgctcgaag agcgcgagga cgtcaatgaa
     6601 gttcatgccg cgcaccgggt caccgccgat gccgacgcag gtgctctggc cgagcccgat
     6661 gttggtcgtc tggaacacgg cctcgtaggt gagcgtgccg gaacgcgaca cgatgccgac
     6721 gctgcccttc ttgtggatga agccgggcat gatgccgatc ttgcactcgt ccggcgtgat
     6781 cacgccgggg cagttcggcc cgaccagccg cgacttcgag tcccggagcg ccgccttcac
     6841 cttcaccatg tcgttcaccg ggatgccctc ggtgatgcac acgatgagcg cgatgcctg
//
LOCUS       DAOMFD010000100         6881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3902_length_6881_cov_34.413419, whole genome shotgun sequence.
ACCESSION   DAOMFD010000100 DAOMFD010000000
VERSION     DAOMFD010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_6881_cov_34.413419"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            85..588
                     /locus_tag="PL152_07010"
     CDS             85..588
                     /locus_tag="PL152_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003121538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HQR49068.1"
                     /translation="MDWKRLFRHLFATRADVQRAFPPATLSEIEAAIAQAEQSHFGEI
                     RIAIEAGLDPVHAWRGRTPRDRALEVFARLGVWDTAANNGVLIYVLLADRAVDIVADR
                     GYEGRVSSEEWAAACRGMESSFGRGEYRQGMLNGLHHVGGIVARHFPVAAGQRNPDEL
                     PNRPVVL"
     gene            603..1754
                     /locus_tag="PL152_07015"
     CDS             603..1754
                     /locus_tag="PL152_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HQR49069.1"
                     /translation="MIRFGTNRWVTPCVRAAAVASLLTLGGCALFEKQAPPPPPPPPP
                     PPVAQAPLATHLFNYEPGVTTVLGEVQVTHSRHEDTLADIARRFDVGYDELHRANPGV
                     DPWLPGEGTTIIVPTQFVIPDGPRDGILINVASMRLFYFPKPAKGAQRVVVTYPIGIG
                     KIGWATPLGTTKVVSKRAGPIWTPPASVRKEHAANGDPLPARVPPGPDNPLGAYAMNL
                     GWPSYLIHGTNKPPGVGMRASHGCIRLYPEDIESLFGEVAVGTKVTVVNQPLLVAWQG
                     DSLYVQSFPVPVEGADDSKAPEPVVYDAATASRIAKAAGEHGASVNGPLAEQVVTQAR
                     GITTPVTRAGLTLQAWLDASRRVTNTVPEGSNWDGVDAPAGGGEQSRAQ"
     gene            1795..3612
                     /locus_tag="PL152_07020"
     CDS             1795..3612
                     /locus_tag="PL152_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49070.1"
                     /translation="MPESNLSGIVVAEAPGADHAIARLPGSACAFVGRTLRGPVNRPV
                     PVRSYAEFQQVFGGLWQPSPMPYAVEQFFENGGRHAVIVRVVNGGRPTTITLPCGAAG
                     SLTLEARSPGSREALRASVDYDNVGANEDDRFNLVVQRVRSAGSEHIEDQEIFRRLST
                     VPGTTRFVTTALQESTLVRVRGDVPPVRPERTYRAGARHPIGYVDSNADGDDGEPLTD
                     YDLIGSAERGSGLCALRASDEFHFLYVPPPARDRDVGPGLLVAAAALCRELRTMLIVD
                     PPAAWTSCDAAIAGLHELGFESDHALMCFPRILAFDRLRGHYESFANGGAVAGVLARL
                     DEQRSPWEAGPDEEVILRPGTRAQLVLTDSERTRLLAHGINPVQSLRSANATAPALRT
                     LARGSGNGLDSALLTRRRRAMLVMNSVERGTRWAMFAASDRNTWHKVTRQVQDFLEPL
                     VAAGLFGPADEPDACYVICDERVNPPQEVAAGCVSLLVGLRASRPGEYFTFLVTHSPE
                     GSRVRPVRSTVLPAGTRMSVPVAPAEPESAGEAHRQRTVAQQLFGYYREPRPVRPAQA
                     ALESAPGPAPLPESAAAGRRDLEAIARFYRDFLGRGQPY"
     gene            complement(3502..>6881)
                     /locus_tag="PL152_07025"
     CDS             complement(3502..>6881)
                     /locus_tag="PL152_07025"
                     /inference="COORDINATES: protein motif:HMM:NF014585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HQR49071.1"
                     /translation="LVTTRGFGDALRIGYQNRPDIFARAIQLPAMLYSAVIEASERVD
                     ASGAVLEPLDERSLADALVAARHDGLRSVAIVFLHSLRNSRHEEIAASLAADAGFGEV
                     VVSHEAAPFTGLVARGDSTVADAYLSPVLDRYVRQFRQELAESHGQPPLLLMQSNGGL
                     VDPDGFRGINGVLSGPAGGVVGLASVAAACGAARLIGFDMGGTSTDVSLYAGELPRRY
                     ATEIDGVRLHAPMMDIHTIAAGGGSIVRYADGRLQAGPDSAGADPGPACYRRGGPATV
                     TDCNVVLGRIRPEHFPRVLGPSGGESLDAESARARLAEIAEAMARDGTSMGVEQLAEA
                     FLTVAVARMANAIRELALGMGQDPTRFSLLPFGGAAGQHACAVADALGMDSMLLDPLA
                     GVLSAHGIGLAQRRCMRRRSVGETCDGAGAARADSTIDSLAEAASRELLRQGVSPAAV
                     EVRRAASLRFAGSDSTIEVTWGAAEAMRAEFTTAHATLYGYRDEGDVVIAECSVEAIE
                     RDGAAVAHDDDGRRHPRAADDHAIAGGIPRVEAWTRGAWHHVPLLTRDELQVGERLTG
                     PALLVEHGATVWIADGWAARVDAAGRVVLARRQPAGRQTVDKGAAADPMRLEVFNALY
                     MHVAEQMGVVLRQTATSVNIKERLDFSCAIFDGDANLVANAPHMPVHLGSMGASVAAV
                     IERHGHDLRPGDSYVLNSPYHGGTHLPDITVVTPVFDAEGRRVHFFTASRAHHADVGG
                     RSPGSMPPDSRSIAEEGALIEPVRIVRDGRLDEALFRRELAAGAWPARNPDQNLADLR
                     AQLAANARGIRELERAAHEHGLDTLLAYMGHVQDNAEACMRRAIRRLRDGRFEYEMDN
                     GQRVAVAVTVDRAVGAATVDFTGTSSQGDDNFNAPRAVTVAAVLYVFRTLIDEPIPMN
                     AGCLRPIRIVVPPGSMLDPAYPHAVVAGNVETSQCIVDALYGALGLQAAAQGTMNNFT
                     FGNQRYQYYETIAGGSGAGDGFDGASGVQTHMTNSRLTDPEVLEGRFPVLLREFAIRR
                     GSGGDGAFRGGDGLVRCVEFREAMTAAILSNHRRVAPFGLAGGAPGSAGENCLRRRDG
                     RSVRLAATEEVAVEPGDRLEIATPGGGGFGERRGTRG"
BASE COUNT          982 a         2450 c         2447 g         1002 t
ORIGIN      
        1 cggcggcggc ggattcggcg gcggaggatg gggcggcggg ggcggaggct tcggcggcgg
       61 cggcgcgtcg gggcggtggt agtcatggac tggaagcgcc tgttccgcca cctgttcgcc
      121 acgcgcgccg acgttcagcg cgcttttcct ccggcgaccc tgtcggagat cgaagccgcg
      181 atcgcgcaag ccgaacagag ccatttcggc gagatccgta tcgcgatcga ggcggggctg
      241 gacccggtcc acgcctggcg cggccggacg ccgcgcgacc gtgccctcga ggtctttgcc
      301 cgtctcgggg tctgggacac cgcggccaat aatggcgtgc tgatctacgt gctgctggcg
      361 gaccgcgccg tggacatcgt tgccgaccgg ggctacgagg gtcgcgtttc gagcgaggaa
      421 tgggccgcgg cctgtcgcgg catggagtcg agtttcggcc gcggcgagta ccggcagggt
      481 atgctgaacg gcctgcacca cgtcggcggg atcgtcgccc gccacttccc ggtcgctgcg
      541 ggccagcgca acccggatga actgccgaac cggcccgtcg ttctttgagg agggcttgct
      601 gcttgattcg ttttggaacc aaccggtggg tgacaccgtg cgtgcgcgcg gcggccgtcg
      661 cgtccctgct cacgctcggc ggctgcgccc tcttcgagaa gcaggcgccg cccccaccgc
      721 caccgcctcc gccgccccct gtggcgcagg cgccgcttgc aacgcacctg ttcaactacg
      781 agcccggagt gaccaccgtg ctcggcgagg tgcaggttac gcactcgcgt cacgaggaca
      841 cgctcgccga catcgcgcgg cgcttcgacg tcgggtacga cgaactgcac cgggcgaacc
      901 cgggcgtgga tccctggctg cctggcgaag gcacgacgat catcgtgccg acgcagttcg
      961 tgatcccgga tgggccgagg gacggcatcc tcatcaacgt cgcgtcgatg cggctgttct
     1021 actttcccaa gccggccaag ggcgcgcagc gggtcgtggt gacctacccg atcggcatcg
     1081 gcaagatcgg ctgggccacg ccactcggca cgaccaaggt cgtctccaag cgcgcgggtc
     1141 ccatatggac accgcctgcg tcggtgcgca aggagcacgc ggccaacggc gatccgctgc
     1201 cggcacgcgt tccacccggg cccgacaatc cactgggcgc ttacgcgatg aacctcggct
     1261 ggccgagtta cctgatccac ggcaccaaca agccgccggg cgtcggcatg cgcgcgagcc
     1321 acggctgcat ccgcctctat cccgaggaca tcgagtcgct gttcggcgag gtcgcggtcg
     1381 gaacgaaggt gaccgtcgtc aaccagccgc tgctcgtcgc gtggcaggga gatagcctgt
     1441 acgtgcagtc gttcccggtg ccggtggaag gcgcggacga ctcgaaggcg ccggaaccgg
     1501 tcgtgtacga cgccgcgacg gcgtcgcgca tcgcgaaggc ggcgggtgag cacggcgcaa
     1561 gcgtcaatgg cccgctggcg gagcaggtcg tgacgcaggc gcgcggcatc acgacgcccg
     1621 tgacacgcgc aggactgacc ttgcaggcgt ggctcgacgc gtcgagacgc gtgaccaaca
     1681 cggtgcccga aggctcgaac tgggacggcg tggacgcgcc ggccggtggc ggggagcagt
     1741 ctcgcgcgca atgacttgtg ctaacgtacg gcaggggcca gcgggagcac cggattgcca
     1801 gagtcgaacc tgagcggcat cgtcgtggct gaggcgccgg gcgccgatca cgccatcgcg
     1861 aggctgccgg ggtccgcgtg cgcattcgtc ggccgcacgc tgcgtggtcc ggtcaaccgg
     1921 cccgttccgg tgcgcagcta cgccgagttc cagcaggtgt tcggcggcct ctggcagccg
     1981 agcccgatgc cctacgccgt cgagcagttc ttcgagaacg gcgggcgcca cgcggtcatc
     2041 gtgcgcgtgg tgaacggcgg gcgtcccacg acgatcacgc tgccatgcgg cgctgccggg
     2101 tcgctcacgc tggaagcgcg cagccccggc tcgcgcgagg cgctgcgcgc atccgtggat
     2161 tacgacaacg tcggtgccaa cgaggacgac cgcttcaacc tcgtcgtgca gcgcgtgcgc
     2221 tccgccggct ccgagcacat cgaggaccag gagatcttcc gtcgcctctc gaccgtgccg
     2281 ggtaccacgc gcttcgtcac gacggcgctg caggagtcga cgctggtgcg cgtgcgcggc
     2341 gacgtgccgc cggtgcggcc ggagcggacg taccgtgccg gcgcgcgcca cccgatcggt
     2401 tacgtggact ccaatgcgga cggggatgac ggcgagccgc tcaccgacta cgacctgatc
     2461 ggctcggcgg agcggggcag tggcctctgc gcactgcggg ccagcgacga gttccacttc
     2521 ctctacgtcc cgccgcccgc ccgcgaccgg gacgtcggcc ccggcctgct ggtggccgct
     2581 gcggcgctgt gtcgcgaact gcgcacgatg ctgatcgtgg atccgccggc tgcgtggacc
     2641 agttgcgatg cggccatcgc cggcctgcac gaactcggct tcgagtccga ccacgcgctc
     2701 atgtgtttcc cgcgcatcct cgccttcgac cggctgcgcg gccactacga atccttcgcc
     2761 aacggcggcg cggtcgccgg agtgctcgcc cggctcgacg agcagcgctc accgtgggag
     2821 gcgggaccgg acgaggaagt gatcctgcgg ccgggcacgc gcgcccaact ggtgctgacc
     2881 gactccgaac ggacgcggct gctcgcccat ggcatcaacc cggtgcagtc cctgcgttcg
     2941 gccaacgcca ccgctcccgc gctgcgcacg ctcgcgcgcg ggtcgggcaa cggcctcgac
     3001 agcgcgctgc tcactcgccg ccgacgcgcg atgctggtga tgaacagcgt cgagcgcggg
     3061 acgcgctggg cgatgttcgc ggccagcgat cgcaacacct ggcacaaggt gacgcggcag
     3121 gtgcaggatt tcctcgagcc actggtggca gccgggctct ttggtcccgc cgatgagccg
     3181 gacgcgtgct atgtgatctg cgacgagcgg gtgaaccccc cgcaggaggt cgcggccggg
     3241 tgcgtgagcc tgctggtcgg gttgcgcgcg tcccgcccgg gcgagtattt cacgttcctg
     3301 gtgacgcact ccccggaagg cagccgcgtg cgacccgtgc gctcgacggt gctgccggcc
     3361 ggcacgcgca tgtcggtgcc ggtcgcaccg gcggagccgg agtctgccgg agaggcgcat
     3421 cgtcagcgca cggtcgcgca gcaactgttc ggctattacc gcgaacctcg accagtgcga
     3481 ccggcgcagg ccgcgctgga atcagccccg ggtcccgcgc cgctccccga atccgccgcc
     3541 gccgggcgtc gcgatctcga ggcgatcgcc cggttctacc gcgacttcct cggtcgcggc
     3601 cagccgtact gaacggccgt cccggcgccg caagcagttc tccccggcgc tgccgggtgc
     3661 gccacccgcg aggccgaaag gcgcgacgcg ccggtgattg gagagaatcg cggccgtcat
     3721 cgcctcgcga aattccacgc agcgcacgag gccgtcgccg ccccggaatg ccccgtcgcc
     3781 gcccgatccg cgccggatcg cgaattcacg cagcaggacc gggaagcggc cctccagcac
     3841 ttccgggtcc gtgaggcgcg agttcgtcat gtgcgtctgc acgccgctcg cgccgtcgaa
     3901 gccgtcgccc gctccggaac cgcccgcgat cgtctcgtag tactggtagc gctggttgcc
     3961 gaacgtgaaa ttgttcatcg tgccctgcgc ggcggcctgc aggccgagcg cgccgtacag
     4021 cgcgtccacg atgcactgcg acgtctcgac attgccagcc acgaccgcgt gggggtacgc
     4081 gggatcgagc atcgatccag gcggcacgac gatcctgatg ggtctcaggc agccggcatt
     4141 catcgggatc ggctcgtcga tcagggtgcg gaacacgtag aggaccgcgg cgaccgtcac
     4201 cgcgcgcggc gcattgaaat tgtcgtcacc ctgggatgac gtgccggtga aatccaccgt
     4261 ggccgcgccc acggccctgt ccaccgtgac cgcgaccgcc acgcgctggc cgttgtccat
     4321 ctcgtactcg aagcggccgt cgcgcaggcg acggatcgcg cggcgcatgc aggcttccgc
     4381 gttgtcctgc acgtgaccca tgtacgcgag gagggtgtcg agcccatgct cgtgcgcggc
     4441 ccgctcgagt tcgcgtatgc cgcgggcgtt ggccgcaagc tgggcgcgca ggtcggccag
     4501 gttctggtcc gggttgcgcg ccggccacgc gccggccgcg agttcgcgcc ggaagagcgc
     4561 ctcgtcgagc cgcccgtcgc ggacgatccg cacgggctcg atgagagcgc cttcctcggc
     4621 gatgctgcga ctgtccggtg gcatcgagcc gggggaccgg ccgccgacgt ccgcgtgatg
     4681 cgcacgcgac gccgtgaaga aatgcacgcg ccggccctcc gcatcgaaca cgggcgtcac
     4741 gaccgtgatg tcgggcaggt gcgtgccgcc gtgatagggc gagttgagta cgtaggagtc
     4801 gccggggcgc aggtcgtgac catgtcgctc gatcacggcg gccacgctcg cgcccatcga
     4861 gccgaggtgg accggcatgt gcggcgcgtt ggcgacgagg ttcgcgtcgc cgtcgaaaat
     4921 ggcgcacgag aagtccaggc gctccttgat gttcaccgag gtcgcggtct gccgcagcac
     4981 cacgcccatc tgctcggcga cgtgcatgta cagcgcgttg aacacttcga gccgcatcgg
     5041 gtcagcggct gctcctttgt cgacggtttg ccttcccgcg ggctgccggc gcgcgaggac
     5101 gacgcgcccc gcggcatcga cgcgggccgc ccagccgtcc gcgatccaga ccgtcgcgcc
     5161 gtgctcgacg agcagcgccg gaccggtcag tcgctcgcca acctgcagct cgtcgcgcgt
     5221 aagcagcggc acgtggtgcc aggcaccgcg ggtccaggcc tcgacccgcg ggatgccgcc
     5281 tgcgattgcg tggtcatccg ccgcgcgtgg atgccggcgc ccgtcatcgt cgtgcgcaac
     5341 ggctgcgccg tcgcgctcga tggcttcgac ggagcattcc gcgatcacga cgtcgccctc
     5401 gtcgcggtag ccgtacagtg tcgcgtgggc agtcgtaaac tcggcgcgca tcgcctcagc
     5461 cgcgccccac gtgacctcga tcgtcgagtc cgaaccggcg aatcgcaggc tcgcggcccg
     5521 ccggacttcg acggccgccg gcgacactcc ctgccgcagc aattcgcgcg acgccgcttc
     5581 cgccaggctg tcgatcgtcg agtctgcgcg tgcggcgccc gcgccgtcgc aggtctcgcc
     5641 gacgcttctg cgccgcatgc agcgccgctg cgcgagcccg attccgtgcg cggacagcac
     5701 gccggcgagc gggtcgagca gcatcgagtc catgccgagc gcatccgcca ccgcgcatgc
     5761 gtgctggccg gccgcgccgc cgaagggcag cagcgagaat cgcgtcggat cctgccccat
     5821 gccgagcgcg agttcgcgga tcgcattggc catgcgcgcg acggcgacgg tgaggaaagc
     5881 ttccgcgagt tgctccacgc ccatggacgt gccgtcgcgc gccatcgctt ccgcgatctc
     5941 ggccagcctg gcgcgcgcgg actccgcgtc cagggattcc ccgcccgacg gccccaatac
     6001 ccgcggaaaa tgctccggcc ggatgcgacc gagcacgacg ttgcagtccg tcaccgtcgc
     6061 aggcccgccg cggcggtagc aggcgggccc gggatcggcg cccgccgaat cgggaccggc
     6121 ctgcagtcgg ccgtcggcgt agcggacgat ggacccgcca ccggccgcga tcgtgtggat
     6181 gtccatcatc ggggcgtgca gccggacgcc gtcgatttcc gtcgcgtaac gccggggcag
     6241 ttcgccggcg tacaggctca cgtcggtgga cgtgccgccc atgtcgaagc cgatgaggcg
     6301 cgccgcgccg caggccgccg cgacggaagc gaggcccacg acgccgccgg cgggtcccga
     6361 cagcacgcca ttgatgccgc ggaagccgtc cggatcgacg agcccaccgt tgctttgcat
     6421 cagcagcagg ggtggctggc cgtggctttc cgcgagttcc tgccggaact gccgcacgta
     6481 tcgatccagg accggcgaca ggtaagcgtc ggcgaccgtg ctgtcgcccc gtgcgacgag
     6541 cccggtgaac ggcgcggcct cgtgcgacac gacgacctcg ccgaagccgg cgtcggcggc
     6601 caggctcgcc gcaatctcct cgtgccgcga attgcgcagg ctgtgcagga acacgatcgc
     6661 gaccgagcgc aggccgtcat gccgcgcggc gacgagcgca tcggcgagcg agcgctcgtc
     6721 gagtggctcg agcacggcac cgctcgcatc gacccgctcg ctcgcctcga tcaccgcgga
     6781 gtacagcatc gcgggcagct ggatcgcgcg cgcaaagatg tcgggccggt tctggtagcc
     6841 gatgcgcagg gcgtcgccga agccgcgcgt cgtgacgagc a
//
LOCUS       DAOMFD010000101         6872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3911_length_6872_cov_27.727299, whole genome shotgun sequence.
ACCESSION   DAOMFD010000101 DAOMFD010000000
VERSION     DAOMFD010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3911_length_6872_cov_27.727299"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..808)
                     /locus_tag="PL152_07030"
     CDS             complement(<1..808)
                     /locus_tag="PL152_07030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HQR49072.1"
                     /translation="MSVVPESKLPNVGTTIFTVMSRLAAECGAINLSQGFPDFQAEPA
                     LFDAMDRHMRAGRNQYAPMAGMPELRQAIVDKVAALYGTRFDPESEVTVTAGATQAIF
                     TAVAAFVRPGDEVIVFEPVYDSYVPAIETVGGTAVFAQLQFPDYAPDWAQVAALITPK
                     TRMIVINSPHNPTGSLLTAVDLEKLAELLRGTDIVVLSDEVYEHIVFDGERHASACGH
                     AELAPRSIVVSSFGKTYHITGWKIGYVVGPAALMAEFRKVHQFNVFTVHTP"
     gene            complement(1118..1855)
                     /locus_tag="PL152_07035"
     CDS             complement(1118..1855)
                     /locus_tag="PL152_07035"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HQR49073.1"
                     /translation="MPGSPVLAFDVGGSFVKAGLVDVAVGTLVGETPKRPTPKTPEDV
                     IELLAAMARELPSAGPVGLAFPSVVKDGIACTAANVDRRWIGTDARALLGARLGRRVA
                     FLNDADAAGIAEMSLGAGRGRAGTVMFLTLGTGIGTAIFTEGRLVPNTELGHLEVRGL
                     EAEHRASAKVRTDRGLDWSEWAAAVNEVLAAYHALLWPDLFVIGGGVTENWERFGSLL
                     TSRAEIVPAHFRNDAGIIGAALAAASA"
     gene            complement(1860..2729)
                     /locus_tag="PL152_07040"
     CDS             complement(1860..2729)
                     /locus_tag="PL152_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR49074.1"
                     /translation="MMQGRSWFRLFSLVVGFAFLYIPIVSLVVYSFNESKLVTVWAGF
                     STKWYAALIHNDQILSAAWLSLKVATVTATIAVVLGTLAGLSLARFGPFRGRTLLSGL
                     TTAPLVMPEVITGLSMLLLFVAMEQLIGWPAGRGMTTIVIAHITFTMAYVTVVVQSRL
                     AQMDDSLEEAAMDLGARPAKVFFLITLPIIFPALLSGWLLAFTLSIDDLVITSFVAGP
                     GSSTLPMVIFSKVRLGVSPDINALATIVVTAVAIGMTVAGIMMARQQKVRERDAQMAL
                     AANQQMIESLKKA"
     gene            complement(2726..3640)
                     /locus_tag="PL152_07045"
     CDS             complement(2726..3640)
                     /locus_tag="PL152_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019340812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HQR49075.1"
                     /translation="MKRVLKRLGITGRGAVTSVPYLWLVLFFLVPFVIVLKISFSSAV
                     IAMPPYEPLLNWASDKVVAVKLNVGNFLFLVQDDLYWKAYLNSIKVAGISTLLCLLIG
                     YPIAYAIARSTPSTRNVLMLMVVLPFWTSFLLRVYAWIGILKNNGLLNNLLIWLGVID
                     EPLVILQTDLAVYIGIVYSYLPFMILPLYANLEKMDLTLLEAAADLGCKPWQAFYKVT
                     VPLSTPGIIAGCLLVFIPAVGEFVIPSLLGDPGMLMIGRVLWTEFFNNRDWPVASAVA
                     IALLMFLVIPIMYYQRAQDAEAGGGLHR"
     gene            complement(3637..4782)
                     /gene="potA"
                     /locus_tag="PL152_07050"
     CDS             complement(3637..4782)
                     /gene="potA"
                     /locus_tag="PL152_07050"
                     /EC_number="7.6.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015417 - ABC-type polyamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter ATP-binding protein"
                     /protein_id="HQR49076.1"
                     /translation="MATSPRATQLKFEPWNDPDAKPYVRIEKVTKKFGDFVAVDDVSL
                     EIYKKEIFCLLGASGCGKTTLLRMLAGFERPTSGRIYIDGVDMTTIPPYERPVNMMFQ
                     SYAVFPHMTVEQNVAFGLKQEGLAKAEIAKRVGDMLDLVKLSQFAKRKPHQLSGGQRQ
                     RVALARSLVKKPKLLLLDEPLAALDKKLREHTQFELINIQETLGVTFIVVTHDQEEAM
                     TLSTRIGLMNHGEIVQVGTPTEMYEYPNSRFVSEFIGSVNMFEGKLTEDEPSYVRIQC
                     DDLDAPIYVDHGVSSAPGATVWAAIRPEKVHLSREKPAQADNWTRAVVRDIAYMGDMS
                     IYILKLESGKLVRVTQPNIHRHADDRITWDERVFLSWHAESPVVVLK"
     gene            complement(4925..6085)
                     /locus_tag="PL152_07055"
     CDS             complement(4925..6085)
                     /locus_tag="PL152_07055"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HQR49077.1"
                     /translation="MKLNLRAGLVGLAAALALVGCGKKQEQAPPAAEAAKPEAAAPAN
                     TEEKVVNVYNWSDYIDPKMLEEFTKQTGIKVNYDVFDNNEVLQTKLLAGNTGYDVVVP
                     SASFLELQIKAGVFQKLDKSLLPNWKYLDKNILERVAKHDPGNQYAVNHMWGTDAIGY
                     NVKKAKAVAPDAPLNSWKVMFDPAWVSKFKSCGVSVLDAPDEVVGIVLAYLGKDPNSE
                     NLDDLKLAEDTLMKIRPYIKTINSSPYIDALANGDLCLVLGWSGDILQARSRAEEAKQ
                     GVEIGMNVPTDGTIIWFDMYAIPADAPHPKNAHAFINFMMEPKVAAANSNFVHYANGN
                     AASIEFLDPAVHNDPGVYPPPEVMAKLFPNLARSQEFTRELNRAWTRFMTGK"
     gene            complement(6210..>6872)
                     /locus_tag="PL152_07060"
     CDS             complement(6210..>6872)
                     /locus_tag="PL152_07060"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR49078.1"
                     /translation="TYWPEIQKICDRHGILLVADEVICGFGRTGHWFGTNLYGLRPDL
                     MTIAKGLSSGYLPIGGVMVADRVADVLIDKGGEFAHGYTYSGHPAACAVASVNLTLIE
                     QEGLVPRTRDETGPYLATKWRALSEHPLVGEARSIGLIGALELVRDKHGRRFFDKRGE
                     VGTICRDFCFGNGLIMRAVRDTMIIAPPLVISREQIDELAEKAWKCLDLTLERCRAEG
                     RL"
BASE COUNT         1146 a         2299 c         2257 g         1170 t
ORIGIN      
        1 acggcgtatg caccgtgaac acgttgaact ggtgcacctt gcggaactcg gccatgagcg
       61 ccgccgggcc gaccacgtaa ccgatcttcc agccggtgat gtggtaggtc ttgccgaagc
      121 tggaaacgac gatgctgcgc ggcgccagtt cggcatggcc gcatgcgctg gcgtgccttt
      181 caccgtcgaa cacgatgtgc tcgtaaactt cgtcggagag cacgacgatg tcggtgccgc
      241 gcagcaattc ggccagtttt tcgaggtcga ccgcagtcag caggctgccg gtcgggttat
      301 gcggcgaatt gatgacgatc atccgcgtct tcggcgtgat cagggcggcc acctgcgccc
      361 agtccggcgc gtaatccggg aattgcagct gcgcgaagac cgccgtgccg ccgacggttt
      421 cgatggccgg cacgtaggag tcgtacaccg gctcgaagac gatcacctcg tcgccggggc
      481 ggacgaaggc ggcaaccgcc gtgaaaatcg cctgcgtcgc gccggcggtg accgtcacct
      541 cgctctcggg gtcgaaacgc gtgccgtaga gcgccgccac cttgtcgacg atcgcctggc
      601 gcagttccgg catgccggcc atcggcgcgt actggttgcg cccggcacgc atgtggcgat
      661 ccatggcgtc gaacagcgcc ggttccgcct ggaaatcggg aaagccctgc gacaggttga
      721 tcgccccgca ctcggccgcg aggcgcgaca tcacggtgaa gatggtcgtg ccgacgttcg
      781 gcagcttcga ttccggcacc acgctcatgc cgagttgcgc ctcggtcccg gatgggagag
      841 ggttctggag acacgccgca agtacgtcca tgtaggctcg cacgcggcgt ccatgccgcg
      901 tgacggtctc cagaaccctc tcccatccgg aaccgcaacg gcgcctggcg tgcgcgtcac
      961 gcgatcgcgg gatcggtgcg agcgcttcgg gtccgagacg attcgggagg ccgtcacgcg
     1021 gcatggacgc cgcgtgcgag cccccaggga tgggttcacg gcgtgcctcc cgaatcgtcc
     1081 ccggctcgcg accagcgcac acccaggtag gcggccctca tgcggaagcc gcggcgagcg
     1141 ccgcgccaat gatgccggcg tcgttgcgga agtgagcggg cacgatctcc gcgcggctcg
     1201 tgagcagcga gccgaagcgc tcccagttct ccgtgacccc gccaccgatg acgaacaggt
     1261 ccggccagag cagcgcgtga tacgcggcga gcacctcgtt gaccgccgcg gcccactcgc
     1321 tccagtcgag cccgcgatcc gtcctgacct tcgccgatgc gcggtgctcg gcttccaagc
     1381 cccggacctc gaggtgaccg agttccgtgt tgggcaccag cctgccctcc gtgaagatgg
     1441 ccgtgccgat acccgtaccg agcgtgagga acatcacggt gccggctcgc ccgcgacccg
     1501 ccccgagcga catctccgcg atacccgcgg cgtcggcgtc gttgagaaag gcgacccggc
     1561 gaccgagccg cgcgccgagc aacgcccgcg catccgtgcc gatccagcgc cggtccacgt
     1621 tcgccgcggt gcaggcgatg ccgtccttta cgaccgatgg aaacgcaagg ccgaccggcc
     1681 cggcggaggg cagctccctc gccatggcgg ccagcaactc gatcacgtcc tcgggagtct
     1741 tcggcgtcgg ccgcttcggg gtctcgccga ccagcgtgcc caccgcaacg tccaccagcc
     1801 ccgccttcac gaatgagccg ccgacgtcga acgcaaggac gggcgacccg ggcatcgagt
     1861 caggccttct tgaggctttc gatcatctgc tggttggccg ccagcgccat ctgtgcgtcg
     1921 cgctcccgga ccttctgctg ccgcgccatc atgatgcccg caaccgtcat cccgatcgcc
     1981 accgcggtca ccacgatcgt cgccagcgca ttgatgtccg ggctcacccc gaggcgaacc
     2041 ttggagaaga tcaccatggg cagcgtgctc gagcccggtc ccgccacgaa gctcgtgatc
     2101 acgaggtcgt cgatcgagag cgtgaacgcc agcagccagc cggaaagcag cgcggggaag
     2161 atgatgggca gcgtgatcag gaagaagacc ttggcgggtc gcgcgccgag gtccatggcc
     2221 gcttcctcga gcgagtcgtc catctgcgcg aggcgcgact gcaccacgac cgtcacgtac
     2281 gccatcgtga aggtgatgtg ggcgatcacg atcgtcgtca tgccgcggcc cgccggccag
     2341 ccgatcagct gctccatcgc cacgaacagc agcagcatcg acaaccccgt gatgacttcg
     2401 ggcatcacca gcggtgccgt cgtcaggccg gagagcagcg tgcggccgcg gaacggaccg
     2461 aagcgcgcca gcgagagccc ggccagcgtt cccagcacga ctgcgatcgt ggcggtcacc
     2521 gtcgcaacct tgaggctgag ccaggccgcg ctcaggatct ggtcgttgtg gatcagggcc
     2581 gcgtaccact tggtcgagaa gccggcccac acggtcacga gcttcgactc gttgaacgag
     2641 tagacgacca gcgagacgat cgggatgtac aggaacgcga acccgacgac gagcgagaac
     2701 aggcggaacc aggagcgacc ctgcatcatc ggtgcagccc tccgcccgcc tcggcgtcct
     2761 gggcacgctg gtagtacatg atcgggatca ccaggaacat gagcagcgcg atcgccaccg
     2821 cgctcgcgac cggccagtca cggttgttga agaactcggt ccacagcacg cggccgatca
     2881 tgagcatccc ggggtcgccg agcagcgacg ggatcacgaa ctcgccgacc gccgggatga
     2941 acaccagcag gcagccggcg atgatgcccg gcgtcgagag cggcacggtc accttgtaga
     3001 acgcctgcca cggcttgcag ccgaggtccg cagccgcctc gagcagcgtc aggtccatct
     3061 tctcgaggtt ggcgtacagc ggcaggatca tgaacggcag gtaggaatag acgatgccga
     3121 tgtacacggc gaggtccgtc tgcaggatga ccagcggttc gtcgatgacg ccgagccaga
     3181 tcagcaggtt gttcagcagc ccgttgttct tgaggatgcc gatccacgcg tagacgcgca
     3241 gcaggaacga cgtccagaaa ggcagcacca ccatcagcat cagtacgttg cgcgtcgagg
     3301 gcgtgctgcg cgcgatcgca taggcgatcg ggtagccgat cagcaggcac agcagcgtcg
     3361 agatcccggc caccttgatc gagttgaggt acgccttcca gtacaggtcg tcctgcacca
     3421 ggaacaggaa gttgccgacg ttcagcttga cggcgacgac cttgtcggag gcccagttga
     3481 gcagcggctc gtacggcggc atcgcgatca ccgcgctgga gaagctgatc ttgagcacga
     3541 tgacgaacgg gaccaggaag aacagcacca gccacaggta aggcaccgag gtcacggcgc
     3601 cgcgcccggt gatcccgagc cgcttgagca cgcgcttcac ttcagcacca ccaccgggct
     3661 ctcggcgtgc cagctcagga acacgcgttc gtcccacgtg atgcggtcgt cggcgtggcg
     3721 gtggatgttg ggctgcgtga cgcgcaccag cttgcccgac tcgagcttga ggatgtagat
     3781 ggacatgtca cccatgtaag cgatgtcgcg cacgacggca cgggtccagt tgtcggcctg
     3841 cgccggcttc tcgcgcgaca ggtggacctt ctcggggcgg atggcggccc agaccgtcgc
     3901 gcccggcgcc gagctcacgc cgtggtccac gtagatcggg gcgtcgaggt cgtcgcactg
     3961 gatccgcacg tacgacggct catcctccgt gagcttgccc tcgaacatgt tcaccgaccc
     4021 gatgaactcg gagacgaagc gcgaattcgg gtactcgtac atctcggtgg gcgtgccgac
     4081 ctgcacgatc tcgccgtggt tcatcagtcc gatgcgtgtc gacagcgtca tcgcctcctc
     4141 ctggtcgtgc gtcaccacga tgaaggtgac tcccagcgtc tcctggatgt tgatgagctc
     4201 gaactgggtg tgctcgcgca acttcttgtc gagcgccgcc agcggttcgt cgagcagcag
     4261 cagtttcggc ttcttcacca gcgagcgggc cagcgccacg cgctgccgct gcccgcccga
     4321 gagctggtgc ggcttgcgct tcgcgaactg cgagagcttc accaggtcga gcatgtcgcc
     4381 gacgcgcttg gcgatctcgg ccttcgccag gccctcctgc ttgagcccga acgcgacgtt
     4441 ctgctccacc gtcatgtgcg ggaacaccgc ataagactgg aacatcatgt tgaccggccg
     4501 ttcgtacggc gggatggtcg tcatgtcgac gccgtcgatg tagatcctgc ccgatgtcgg
     4561 tcgctcgaac ccggccagca tgcgcagcaa cgtggtcttg ccgcagccgg aggccccgag
     4621 caggcagaag atctccttct tgtagatctc gaggctgacg tcgtccaccg cgacgaagtc
     4681 gccgaatttc ttggtgacct tctcgatgcg cacgtagggc ttcgcatcgg ggtcgttcca
     4741 gggctcgaat ttcagctggg tcgcgcgcgg tgaagtcgcc atgtcgcctc gggcttcttg
     4801 ggtcggtgcg gccgtcagcc cgggttatag cccagggccg cggacgatgg gcaggcctcg
     4861 aacgaacgcg gggcggacgg agttgtgctc cggccgcccc gcgtggattc cattcgtcct
     4921 ggccttactt gccggtcatg aaccgcgtcc aggcgcggtt cagctcgcgc gtgaactcct
     4981 gcgaccgcgc gaggttcggg aacagcttcg ccatcacctc gggcggcggg tagacacccg
     5041 ggtcgttgtg cacggccgga tcgaggaatt cgatcgacgc ggcgttgccg ttggcgtagt
     5101 ggacgaagtt ggagttcgcc gccgccacct tcggctccat catgaagttg atgaaggcgt
     5161 gcgcgttctt cgggtgcggc gcgtcggccg ggatggcgta catgtcgaac cagatgatcg
     5221 tgccgtcggt cggcacgttc atgccgatct cgacgccctg cttggcctcc tcggcacggc
     5281 tgcgcgcctg caggatgtcg ccggaccagc cgaggaccag gcacaggtcg ccgttcgcca
     5341 gcgcgtcgat gtacggcgag ctgttgatcg tcttgatgta cggccggatc ttcatcagcg
     5401 tgtcttcggc gagcttcagg tcgtcgaggt tctcgctgtt cgggtccttg ccgaggtagg
     5461 cgagcacgat gccgaccacc tcgtccgggg cgtcgagcac ggagacgccg cagctcttga
     5521 acttcgacac ccaggccgga tcgaacatca ccttccagct gttcagcggc gcatccggcg
     5581 ccaccgcctt ggccttcttg acgttgtagc cgatcgcgtc ggtgccccac atgtggttca
     5641 ccgcgtactg gttgcccggg tcgtgcttcg ccacgcgctc caggatgttc ttgtccaggt
     5701 acttccagtt cggcagcagc gacttgtcga gcttctggaa cacgccggcc ttgatctgca
     5761 gttcgaggaa cgaggccgag ggcaccacga cgtcatagcc ggtgttgccg gcgaggagct
     5821 tggtctgcag cacctcgttg ttgtcgaaca cgtcgtagtt gaccttgatg cccgtctgct
     5881 tggtgaattc ctcgagcatc ttcgggtcga tgtagtccga ccagttgtag acgttgacga
     5941 ctttctcctc ggtgttcgcc ggagcggcgg cctcgggctt ggccgcctcg gcggccggcg
     6001 gcgcctgctc ctgcttcttg ccgcaaccga cgagcgcgag tgccgccgcc aggccgacga
     6061 gcccggcgcg aagattgagt ttcatcgtgt taccccgtat gtcgatgtgc atcggcgcgg
     6121 ccggcgagcc gctccccttg aaagcatgta tatcgcaccg gacggggcta gacaaggaat
     6181 ggccagcccc gctccccccg ggccgtcact cagagccggc cctctgcccg gcaccgctcg
     6241 agtgtcaggt ccaggcactt ccaggccttc tccgccagct cgtcgatctg ctcgcggctg
     6301 atcaccagcg ggggcgcaat gatcatggtg tcgcgcacgg cccgcatgat gagcccgttc
     6361 ccgaagcaga agtcccggca gatcgtgccg acttcccctc gcttgtcgaa gaagcgacgg
     6421 ccgtgcttgt cgcggaccag ctccagcgcc ccgatcaggc cgatcgaccg ggcctcgccg
     6481 accagcgggt gttccgacag cgcccgccac ttcgtcgcga ggtacggacc cgtctcgtcg
     6541 cgggtccgcg gcacgagccc ctcctgctcg atcaaggtca ggttgacgct cgccacggcg
     6601 caggcggccg ggtgccccga gtaggtgtag ccgtgcgcga attcgccacc cttgtcgatc
     6661 aggacgtccg cgacacggtc cgcgaccatg acgccgccga tcggcaggta tcccgacgac
     6721 agccccttgg cgatcgtcat gaggtccggc cgcaggccat acaggttggt gccgaaccag
     6781 tggccggtgc ggccgaaacc gcagatgacc tcgtcggcga cgagcaggat cccgtggcgg
     6841 tcgcagatct tctggatctc cggccagtag gt
//
LOCUS       DAOMFD010000102         6820 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_3965_length_6820_cov_37.428825, whole genome shotgun sequence.
ACCESSION   DAOMFD010000102 DAOMFD010000000
VERSION     DAOMFD010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3965_length_6820_cov_37.428825"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..873
                     /locus_tag="PL152_07065"
     CDS             <1..873
                     /locus_tag="PL152_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HQR49079.1"
                     /translation="FRDHILPDRIHLLNVSFLVPSMRRNFGGCAGNIAYSLRLLGGQG
                     IPMATVGHDFGPYGQWLRERSVDVSQLRVVDGEYTAQAFITTDLDDNQITAFHPGAMN
                     QSHRNEVSQVTGCSIGLVAPDGREGMVQHAAQFAAAGIPMLFDPGQGLPMFDGAELLD
                     FISKATWVAVNDYEGQLLSERTGLSHTEIANRVQALIVTKGAEGSVIHAAGREYRIPA
                     ARPDAVVDPTGCGDAYRAGLLFGLMNGLDWETTGRIAALLGAIKIAHAGTQAHSFTMD
                     EFGDRFRAAFGRAL"
     gene            885..>3343
                     /locus_tag="PL152_07070"
     CDS             885..>3343
                     /locus_tag="PL152_07070"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQR49080.1"
                     /translation="MRALALAVLVAAAAVPRLAAAESQVWDKTLARVAPSVVSIQIDQ
                     ARAFDTEWNESSQATGFVVDAERGLILTNRHVVTPGPVIATAVFQNREEVELKPVYRD
                     PVHDFGFYRYDPSKLRYAKPASLPLDPAGARVGVEIRVVGNDAGEQLAILGGTIARLD
                     RDAPDYGVGKYNDFNTFYIQAASGTSGGSSGSPVIDIRGHVVALNAGGSTGAAASFFL
                     PLGRVQRALDLIREGKPVTRGTLQTIFKYTPYDELVRLGLRPETEAAARRAAPGLTGM
                     LVVSEVQPGSPADGQLQPGDILVKVNGKRVATFDPLAETLDSSVGRTVHVEVERLGAT
                     LERDLVVQDLASITPSRYLQFGDAVVHALSWQMARHMNAPVRGVFVANPGYSLGAAGV
                     PRGAVISSVAGRPVETLDDFEQVIAGLPDGERAALRYSTLDDPKGSETRVMRMDWQWF
                     PAQDCTRDDAAGNWSCRDLAAPTAAAAEAPARTSFATTGDAVVDRLAPSLVLVNFDMP
                     YSVAGVTERSYHGTGLVVDAKRGLVAVDRNTVPVAVGDVRLTFAGSVEVPGRVEYVHP
                     LHNVAIVSYDPALVDGTPVRTASLDPRPLEAGEAVTVVGLTGDSRVQSLATTVATVDD
                     VAFPLSRTLEFREANLEVGTLVNGPTTFDGAIVGRDGRVRAMWASFAYEGGRELSQVS
                     RAMPIDIVEEAVAAARGKPLYSLEVEFDTVSIAEARKLGLPADWVVKLEKDSGPHRQA
                     LRVTRVVAGSPAAAVLRPGDLLLAVNGRAVSRFREVELATQSPSVGLTVLRDGKEAQL
                     DVATVALGGKDIDRLLIWAGAT"
     assembly_gap    3344..3443
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3444..4471
                     /locus_tag="PL152_07075"
     CDS             <3444..4471
                     /locus_tag="PL152_07075"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HQR49081.1"
                     /translation="PQDEIDRAVEALREGEVIAFPTETVYGLGADAQNSAAVTRVYEL
                     KGRPRTHPLIVHIDHPRMLERWALSIPPAAEALAARFWPGPLTLVLRRAPAVDLGVTG
                     GQDTVAIRVPGHPVAQQLLRAFGSGIAAPSANRYGHVSPTRAEHVRDEFGTAVPLILD
                     GGDCKIGLESTIVDCVDTVPRVLRPGFITLSQLRAVVPEIQGRADSESPRVPGSDVKH
                     YAPTTPLSVVQSATLEEVVSQLTGDHARVAVLATRPPRTANKFMTWVNAGRRADTYAR
                     ELYVNLRTLDKSGAREILVEAVPEGEAWDAVRDRLHRAASAENVVTVDPDIAALRADL
                     GEDVGPS"
     gene            4602..5684
                     /locus_tag="PL152_07080"
     CDS             4602..5684
                     /locus_tag="PL152_07080"
                     /inference="COORDINATES: protein motif:HMM:NF014803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III PLP-dependent enzyme"
                     /protein_id="HQR49082.1"
                     /translation="MLVIDCEAIRAQYSSLSAALPGVALHYALKPLPFGPVVAALRDL
                     GSGFDVATTGEIALVRAQGVAPERCIHTHPIKTHRDIAAALRFGIRTFVADNPDELRK
                     FARHARRAEVLLRVSFRNPAAVVDLSRKFGCEPAAVLPLLEVARGLGVRVRGLSFHVG
                     SQVADPTRFVEAIDACREIMLEARAAGHALDVLDIGGGFPVAYGGRLPGIREFCRPIR
                     RELKSLPAGTRVIAEPGRFIAATAGTCIASVVGRAQREGRWWYYLDDGVYGSFSGQLY
                     DHALYPVDSLDRQGPRHPCVLAGPTCDSIDVIREDIPLPDLQIGDLLSGRMMGAYTQA
                     TATDFNFIPRARIVAVNQRGGRHHAD"
     gene            5674..6372
                     /locus_tag="PL152_07085"
     CDS             5674..6372
                     /locus_tag="PL152_07085"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HQR49083.1"
                     /translation="MRIEMFDHVAGAAAARGIAVVIDVFRAFSVAPHAFDRGAARVFP
                     VGEIEEAIALGRRFPGSVLAGERHARRLPGFDAGNSPTEIRALDLRGRVLVHTTHAGT
                     QGLVNAAGADEVLTGAFVNISAVAHHIMAIAPPLVSLVRMGHEARERCAEDDLYAECL
                     AAILSGRPAPLGEVRERLREAPAARKFFDPDCDWAPREDFDYCTEVDRFDFVLRLKRV
                     PGEPLELERIARGG"
     gene            6387..>6820
                     /locus_tag="PL152_07090"
     CDS             6387..>6820
                     /locus_tag="PL152_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49084.1"
                     /translation="MEFVLFGSTLLGVALFHDRTLEVAATGLAAIIAYKLALADFDGL
                     PGFAGLAHHFRSEQVGLANLFCLLMGFALLSRHFEESRVPARLPDFLPDDWRGGFVLL
                     VLVFVLSSFLDNIAAALIGGTVAATVYRHKVHVGYLAAIVAAS"
BASE COUNT          924 a         2327 c         2429 g         1040 t
ORIGIN      
        1 ttccgggacc acatcctgcc ggaccgcatc cacctgctga acgtgtcgtt cctcgtgccg
       61 agcatgcgcc gcaacttcgg cggctgcgcc ggcaacatcg cctacagcct gcgcctcctc
      121 ggcggccagg gcatcccgat ggcgaccgtc ggccacgact tcgggccgta cgggcaatgg
      181 ctgcgcgagc gctcggtgga cgtgtcgcag ctgcgcgtgg tcgacggcga gtacacggcc
      241 caggcgttca tcaccaccga cctcgacgac aaccagatca ccgcattcca ccccggcgcg
      301 atgaaccaat cgcaccgtaa cgaggtctca caggtcaccg gctgctcgat cggtctggtg
      361 gccccggacg gacgcgaggg catggtgcag cacgccgcgc agttcgcggc ggcggggatc
      421 ccgatgttgt tcgacccggg ccagggattg ccgatgttcg acggtgcgga actgctcgat
      481 ttcatttcca aggcgacctg ggtcgccgtg aacgactacg agggccagct gctgtcggag
      541 cgcacgggcc tgtcgcacac cgagatcgcg aaccgggtcc aggcgctgat cgtgaccaag
      601 ggcgcggaag gcagcgtgat ccacgccgcc ggccgagagt atcggattcc cgctgcccgg
      661 cccgacgccg tggtggaccc caccggctgc ggcgatgcct atcgcgccgg gctgctcttc
      721 ggcctcatga acgggctcga ctgggaaacg acgggacgta tcgctgcgct gctgggcgcg
      781 atcaagatcg cccacgccgg cacgcaggcg cactcgttca cgatggacga attcggcgac
      841 cggttccgcg cggcattcgg tcgcgcgctg tgaccgtgcg tcgcatgcgt gccctggcgc
      901 tggcggtgct cgtcgccgcc gcggccgtcc cgcggctcgc agccgccgag agccaggtct
      961 gggacaagac cctggcgcga gtcgcgccgt cggtcgtctc catccagatc gaccaggcgc
     1021 gcgcgttcga caccgagtgg aatgagtcca gccaggcgac cggcttcgtg gtggatgccg
     1081 agcgtggcct gatcctcacc aaccgccacg tcgtgacacc cgggccggtg atcgcgacgg
     1141 ccgtgttcca gaaccgtgag gaagtggagc tcaagccggt gtaccgggac ccggtgcacg
     1201 acttcgggtt ctatcgctac gatccgtcga agctgcggta tgcgaagccc gcgtcgctgc
     1261 ccctcgaccc ggccggtgcg cgcgtcggcg tggagatccg cgtcgtgggc aacgatgcgg
     1321 gcgagcagct tgcgatcctc ggcggcacga tcgcgcgcct cgaccgtgac gctcccgact
     1381 acggggtcgg caagtacaac gacttcaata cgttctacat ccaggcggcg tccggaacct
     1441 cgggcggctc gtcgggctcg ccggtcatcg acatccgcgg gcacgtagtt gcactgaatg
     1501 ccgggggcag cacgggcgcc gctgcgagct tcttcctgcc gctcgggcgc gtgcagcgcg
     1561 cgctcgacct gattcgcgaa ggcaagccgg tcacgcgcgg tacgctgcag accatcttca
     1621 agtacacgcc gtacgacgaa ctggtgcggc tgggactgag gccggagacc gaggcggccg
     1681 cgcggcgcgc tgcgcctggt ctcacgggca tgctcgtcgt gtcggaagtg cagccgggat
     1741 cgccggcgga cggccaactc cagcccggcg acatcctcgt caaggtgaac ggcaagcggg
     1801 tcgccacgtt cgatccattg gctgagacgc tggactcctc cgttggccgc acggtacacg
     1861 tcgaggtgga gcgcctcggc gccacgctcg agcgcgacct cgtcgtgcag gacctggctt
     1921 cgatcacgcc ctcgcggtat ctgcagttcg gcgacgccgt cgtgcacgcc ctgtcgtggc
     1981 agatggcgcg acacatgaat gcgccggtgc gcggggtgtt cgtcgccaat cccggctatt
     2041 cgctgggcgc ggccggggtg ccgcgcgggg ccgtcatcag cagcgtggcc gggcgcccgg
     2101 tggagacgct ggacgacttc gagcaggtga tcgccggtct tccggacggc gagcgggcgg
     2161 cgctgcgcta ctcgacgctc gacgacccga agggcagcga aacgcgcgtg atgcgcatgg
     2221 actggcagtg gttcccggcg caggactgca cgcgagacga cgcggccggc aactggtcgt
     2281 gccgcgacct cgcggcgccg actgcggccg ccgccgaggc gccggcgcgg acgtcgttcg
     2341 cgacgaccgg agacgccgtc gtcgatcggc tcgcgccgtc gctggtcctg gtgaacttcg
     2401 acatgccgta ctcggtcgcg ggcgtcaccg agcgcagcta ccacggcacg ggcctggtcg
     2461 tggacgcgaa gcggggcctc gtggcggtgg atcgcaacac cgtgcccgtc gcggtcggcg
     2521 acgtgaggct cacgttcgcg ggctcggtcg aggtcccggg gcgggtcgag tacgtgcacc
     2581 cgctgcacaa cgtcgccatc gtctcctacg acccggcgct ggtcgacggc acgccggtcc
     2641 ggaccgcgtc actcgaccct cgtccgctcg aggcgggcga ggcggtgacg gtcgtgggcc
     2701 tgaccggcga ttcgcgcgtg cagtcgctcg cgacgacggt cgcaaccgtg gacgacgtgg
     2761 ccttcccgct gtcgcggaca ctggaattcc gggaagccaa cctcgaggtc ggcacgctgg
     2821 tcaacggccc cacgacgttc gacggcgcca tcgtcggacg cgacgggcgc gttcgggcca
     2881 tgtgggcgag cttcgcgtac gaaggcgggc gggagctctc gcaggtgagc cgggccatgc
     2941 cgatcgacat cgtcgaggag gcggtcgcgg cggcgcgcgg caagccgctc tattcgctgg
     3001 aagtcgaatt cgacacggtt tccatcgccg aggcgcgcaa gctcgggttg cccgccgact
     3061 gggtggtgaa actggagaag gacagcggcc ctcaccgcca ggcactgcgc gtcacgcggg
     3121 tggtggcggg ctcgccggcc gccgcggtcc tgcgtcccgg cgacctgctg ctcgcagtga
     3181 acggacgcgc ggtgagtcgc ttccgcgaag tcgagcttgc gacccagtcg ccctccgtgg
     3241 gactgacggt gctgcgcgac gggaaggagg cccagctcga cgtcgcgacg gtcgcgctcg
     3301 gcggcaagga catcgaccgg ctgctcatct gggccggggc gacnnnnnnn nnnnnnnnnn
     3361 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3421 nnnnnnnnnn nnnnnnnnnn nnntgccgca ggatgagatc gaccgcgccg tggaggcgct
     3481 gcgcgaaggc gaggtcatcg cctttccgac ggagaccgtc tacggcctgg gggccgacgc
     3541 ccagaattcg gccgcggtga ccagggtcta cgagctcaag ggccggccgc ggacccaccc
     3601 gctgatcgtg cacatcgacc atccgcggat gctggagcgc tgggccctgt cgattccgcc
     3661 cgctgccgag gcgctggcgg ccaggttctg gcccgggccg ctgacgctgg tcctgcgccg
     3721 cgcgccggcc gtggacctgg gcgtgaccgg tggacaggac accgttgcga tccgcgtccc
     3781 gggacacccg gtggcccagc aactcctgcg tgctttcggg agcggcatcg ccgcgccttc
     3841 ggccaaccgt tacggccacg tgagcccgac gcgcgcggag cacgtgcgcg acgagttcgg
     3901 cacggcggtg ccgctgatcc tcgacggcgg ggactgcaag atcggcctgg agtccacgat
     3961 cgtcgactgc gtggacaccg tgccgcgggt actgcggccg ggattcatca cgctgtcaca
     4021 gctgcgcgcg gtcgtgcccg agatccaggg acgggccgac agcgaatcgc cgcgcgtgcc
     4081 ggggtccgac gtcaagcact atgcacccac gaccccgctc agcgtcgtcc agagcgccac
     4141 gctcgaggag gtcgtgagcc agctcaccgg cgatcacgcc cgggtcgcgg tgctcgcgac
     4201 ccgcccgccg cggacggcca acaagttcat gacctgggtc aacgcggggc ggcgcgccga
     4261 cacttatgcg cgggagctgt acgtgaacct gcgcacgctg gacaagtccg gcgcgaggga
     4321 gatcctcgtc gaggccgtgc cggagggcga ggcctgggac gccgtgcgcg atcgcctgca
     4381 ccgagcggca tccgccgaga acgtggtcac ggtggatccg gatatcgctg ccttgagggc
     4441 ggatctcggc gaggacgttg ggccatcatg acgccagggc ccggtatgcc ggccgagtcc
     4501 cgtttgtgaa gcgcgtgttg ccgcccaggt cgcggcgccg agcgcggccg cggatcacgc
     4561 cgagtgaact gcgcaggctc gcgcgcagcc atgggacgcc gttgctcgtc atcgattgcg
     4621 aggcgatccg cgcgcagtac tcgtcgttgt cggcggcgct gcctggcgtc gcgctgcact
     4681 atgcgctgaa gccattgccg ttcggcccgg tggtcgcggc gctccgcgac ctcgggtccg
     4741 gattcgacgt cgcgacgacg ggcgagattg cgctcgtgcg tgcgcagggc gtcgcccccg
     4801 agcgctgcat ccacacgcat ccgatcaaga cccacaggga catcgccgcg gcgctgcgct
     4861 tcgggatccg gaccttcgtc gccgacaacc cggacgagct gcgcaagttc gcccgccacg
     4921 cccgccgggc cgaggtcctg ctgcgggtgt cgttccgcaa tcccgcggcg gtcgtggacc
     4981 tgtcgcgcaa gttcggctgt gagccggcgg ccgtgctgcc gctgctcgag gtggcacgcg
     5041 gcctcggcgt gcgggtgcgc ggcctgtcgt tccacgtcgg ctcgcaggtc gcggacccga
     5101 cgcgtttcgt cgaggcgatc gacgcgtgcc gcgagatcat gctcgaggcg cgggccgccg
     5161 ggcatgcgct cgacgtgctc gacatcggcg gcggcttccc ggtcgcctac ggcggcaggc
     5221 tgccgggcat ccgcgagttc tgtcgcccga tccgccgcga gctcaagtcg ctgccggcag
     5281 gtacgcgcgt gatcgctgag cccggacgct tcattgccgc caccgctggc acgtgcatcg
     5341 catcggtcgt cgggcgtgcg cagcgcgagg ggcgctggtg gtactacctc gacgacggcg
     5401 tctacggttc gttcagcggc cagttgtacg accacgccct gtatccggtg gacagcctcg
     5461 accggcaggg gccgcgccac ccgtgcgtgc tggcaggccc gacctgcgac agcatcgacg
     5521 tcatccgcga ggacattccg ctgcccgacc tgcagatcgg ggacctgctg tcgggccgga
     5581 tgatgggcgc gtacacgcag gccacggcga cggatttcaa cttcatcccg cgggcacgca
     5641 ttgtcgcggt caaccagcgg ggcgggcggc atcatgcgga ttgaaatgtt cgatcacgtc
     5701 gcgggcgcgg ctgcggcacg tggcatcgcc gtcgtcatcg acgtgttccg cgccttttcg
     5761 gtcgcgccgc acgcgttcga ccggggcgct gcgcgcgtgt ttcccgtcgg ggagatcgag
     5821 gaggccatcg cgctcggccg acgctttccg gggtcggtcc tggccggcga gcgccacgcc
     5881 cgtcggctgc cgggattcga tgccgggaat tcgccgaccg agatccgcgc gctcgacctc
     5941 cgcggcaggg tgctcgtgca cacgacccat gccggcacgc aggggctggt caatgccgcg
     6001 ggcgccgacg aggtgctgac cggcgcgttc gtgaacatct cggccgtggc gcaccacatc
     6061 atggcaatcg cgccaccgct ggtgtcgctg gtgcggatgg ggcacgaggc acgcgagcgc
     6121 tgcgccgagg acgacctgta cgcggagtgc ctcgccgcga tactgagcgg ccggccggcg
     6181 ccgctcggcg aggtccgtga gcggctgcgc gaggcgcccg ccgcccgcaa gttcttcgac
     6241 ccggactgcg actgggcgcc gcgcgaggac ttcgactact gcaccgaggt cgatcgcttc
     6301 gatttcgtgc tgcggctgaa gcgcgtgccc ggtgagccgc tggaactcga gcggatcgct
     6361 cgaggaggct gacggcgttg ccgcccgtgg agttcgtcct gttcggctcg acgctgctcg
     6421 gcgtggcact cttccacgac cgcacgctcg aagtggcggc caccgggctc gcggcgatca
     6481 tcgcctacaa gctcgcgctc gcggacttcg acgggctccc ggggtttgcg gggctcgcgc
     6541 accatttccg ctccgagcag gtcgggcttg cgaacctgtt ctgcctgctc atgggtttcg
     6601 ccctgctgtc gcggcacttc gaggagagcc gggtgccggc ccgcctgccg gacttcctgc
     6661 cggacgactg gcgcggcggc ttcgtgctgc tggtgctggt cttcgtgctg tcgagcttcc
     6721 tcgacaacat cgcggccgcg ctgatcggcg gcacggtcgc cgcgaccgtg taccggcaca
     6781 aggtccacgt cggctatctc gccgcgatcg tcgcggcgtc
//
LOCUS       DAOMFD010000103         6781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4004_length_6781_cov_31.498513, whole genome shotgun sequence.
ACCESSION   DAOMFD010000103 DAOMFD010000000
VERSION     DAOMFD010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4004_length_6781_cov_31.498513"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..712)
                     /locus_tag="PL152_07095"
     CDS             complement(<1..712)
                     /locus_tag="PL152_07095"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HQR49085.1"
                     /translation="MAIAFLGGTCCLMLLDALPGTPLVASLLAAGTVLALSARWWAPL
                     AFASGFTWCWLVASDRLEQRLDPAAEGKNIELRGVVVSVPQRLAGGVRFRLATDAAGG
                     LPPLIELTWYEPTFVPRVAERLDVVARLRRPRGFANPGGMDQEARMLREGIGATGYVR
                     SAASAGRGLDADMRHPVLVVRGAADTTIRRVLGNRPATGIVEGLAVGLQDALSQEQWR
                     ALARSGTSHLMAISGMHIG"
     gene            complement(808..1491)
                     /gene="lolD"
                     /locus_tag="PL152_07100"
     CDS             complement(808..1491)
                     /gene="lolD"
                     /locus_tag="PL152_07100"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HQR49086.1"
                     /translation="MTDAEILSCRGVSKHFDEGGQRLVVLAGVDFAIDRGETVAIVGA
                     SGSGKTTLLQILGGLDLPTTGTVSIAGQSLAELSDAARGTLRNRALGFVYQFHHLLPE
                     FTALENVAMPLLVRRTPVAEAEGAARRLLERVGLGARLVHRPAQLSGGERQRAAVARA
                     LVTRPQLVLADEPTGNLDGVNARAVLDLMLELNRELGTSLVIVTHAAEIAARMQRVLT
                     LSDGRLVAG"
     gene            complement(1484..2743)
                     /locus_tag="PL152_07105"
     CDS             complement(1484..2743)
                     /locus_tag="PL152_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HQR49087.1"
                     /translation="MSAATPFEIRIGRRYLRASGNRFLSFISLMSMLGVTIGVAVLIV
                     VLSVMNGFETELRSRILSITSHATLSAFAAGAEDWPGMRRTALKDPRVAAAAPYVEGE
                     ALLIADRPQGAAAPATVRGVDPQLEATVSAIADRLLSGSLDALRPGSYRMLLGSELAS
                     HLGVAPGDSVVMAIAQGSVTPAGVVPRMRRYTVAGIVRSGMYEIDNGLALTSLADAQR
                     MFRLGDRVTGLRLAMRDPYQAPVVSRDVARALGGGLWVDDWTQRQANFFRSIELTKRM
                     MFFILLLVVAVAAFNIVSTLVMAVKDKQPDIAILRTIGAKPASVLGIFATQGTVIGFL
                     GTLGGVALGVLLSVNLEALVHGLERLLGTQFMDASVYLMSDLPARVEAGDVVLIAGTA
                     FALCCLSTLYPAWRAARTNPARALRHD"
     gene            complement(2743..3990)
                     /locus_tag="PL152_07110"
     CDS             complement(2743..3990)
                     /locus_tag="PL152_07110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HQR49088.1"
                     /translation="MFHPLPMYVGLRYVRTRRQGFFVSFISWVSMLGVCLGVAALITI
                     ISVMNGFEGELRGRLVALTAHATVTGPPAAMADWRHVAERARAVPGTLGAAPFVDVLA
                     MAGRSGQLLPVVLHGVDYRAEPGVSSVEEHLLEGRLADLTPGSRCAIVGRALAWQLGA
                     RVGDQLTVMVPGRDLLTGAGRPTLQTFEVCGIFEVGLQDRDSKLALVAMADAASLAGS
                     GATPAGIRLRFDDVMAAPRRAAAVAAALGDGFRVRDWSQEHAAYFRAIRIEKTMMSLI
                     LMLVVAVAAFNIVAALVMVVNEKRSDIAILRTLGLAPRGVVGIFLTQGIVIGAIGTVL
                     GTLLGVLVAANVGVIVPALERTFGFHVMDPTVYYITELPSELRAAQVMVIAGIAFALT
                     VLATIYPAMRGAATEPAEALRYE"
     gene            4158..4454
                     /locus_tag="PL152_07115"
     CDS             4158..4454
                     /locus_tag="PL152_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49089.1"
                     /translation="MRKTVLAVCAMSLGLGLGTACARADQLALGPSSQTVASVPDRGL
                     SMAAVEKRYGAPSDRLAAIGKPPITRWVYPSFVVYFEYNLVIHSVATQPAAATN"
     gene            4684..>6781
                     /locus_tag="PL152_07120"
     CDS             4684..>6781
                     /locus_tag="PL152_07120"
                     /inference="COORDINATES: protein motif:HMM:NF012492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HQR49090.1"
                     /translation="MLLVARDAASATRLEEELRFFVPAGLSVLAFPGYETLPYDQFSP
                     HPDIISQRLRTLSRLPSLGAGIVVTDVPTTLHRLPPRSFVDGHAIVIEVGQELDLGAF
                     RLRLAAAGYASVPQVGAPGEFAIRGSLFDVFPMGADAPLRIDLLDRQVDSVRSFDPDT
                     QRSGDRLPRIELLPAREFGLGPDSTRDFRRRYRTRFEGDLTRMTLYRDVGDGHAPAGI
                     EYFLPLFFDATASLFDYLPPGAVVALPSDHRRLLDAAWASVEERYEERRYDIEHPVLA
                     PAEAFLSPAEWSDAATRSPRLLLGDTVDEGETVAVPAVDLGTTEAPPLKIDQRRDESI
                     REVAARLAAHPGRVLLAAESPGRRELLLELLRPHGTPVRVVSGWQEFIAGDAPLALTV
                     APLASGVAIPTAHMTVYAEEQLFGERARQERRRRRSDRDPARIIQQLADLRPGAPVVH
                     EDYGVGRYLGLATMDAGGVASEFLVLEYADGDKLYVPVQALERVSRYTGAPAESAPLH
                     KLGGDQWAKARSRAAARIRDAAAELLDVYARRAARQGHSFEFDESQLQAFAAGFPFEE
                     TADQAAAINQVLEDLRSAKPMDRVVCGDVGFGKTEVALRAAFVAVQGGKQVAVLVPTT
                     LLAQQHYQTFADRFADWPMRVELLSRFRGDAQAKSALVGLAEGRVDIVIGTHRLLQPG
                     VRFKDLGLLIIDEEHRF"
BASE COUNT         1044 a         2448 c         2316 g          973 t
ORIGIN      
        1 tgccgatgtg cattccggag atcgccatca ggtggctggt gccgctgcgc gccagggctc
       61 gccactgttc ctggctgagc gcgtcctgca ggcccacggc gagtccctcg acgatcccgg
      121 tggcaggccg gttgccgagc acacgccgaa tcgtcgtgtc ggccgcgcct cgcaccacga
      181 gcaccggatg ccgcatgtcg gcgtcgagac cacggcccgc gcttgcagcc gagcgcacgt
      241 agccggtcgc gccgatgccc tcgcgcagca tgcgggcctc ctggtccatg ccgcccgggt
      301 tggcgaagcc gcgcggccga cgcagtctcg cgaccacgtc caaccgctcg gccacgcggg
      361 gcacgaacgt cggctcgtac cacgtcagct cgatgagtgg tggcagacct ccggccgcat
      421 ccgtcgcgag acgaaagcga acgcctcccg cgagccgctg cgggacggag acgacgactc
      481 cgcgcagttc gatgttcttg ccttccgctg ctggatcgag gcgctgctcc agccggtcgg
      541 acgcgaccag ccagcaccac gtgaacccgg aggcaaacgc cagcggagcc caccatcgag
      601 ccgacagtgc cagcaccgtc ccggctgcca gcagcgacgc aacgagcgga gtgcccggca
      661 gcgcatcgag caacatcagg cagcaggtgc caccgagaaa ggcgatcgcc acgcggacca
      721 cgacccggca ctccgtcggc aggaaccgcc agactgcctc gccaccgcgc gcatcgcgcg
      781 gctcaattca gccgcgtttg cgccatttca gccggcgacg agccggccgt cgctgagcgt
      841 cagcacgcgc tgcatgcgcg ccgcgatctc cgcggcgtgg gtcacgatca cgaggctcgt
      901 gcccagttcg cgattgagct caagcatgag gtcgagcact gcgcgggcgt tcacgccgtc
      961 caggttgccg gtcggctcgt cggcgaggac gagctgcgga cgcgtgacca gggcgcgcgc
     1021 gacggcggcg cgctggcgct cgccgcccga cagctgcgcc ggccggtgca cgagcctcgc
     1081 gccgagtccg acccgctcga gcaggcgacg cgcggccccc tcggcctcgg cgaccggcgt
     1141 gcgccgcacc agcagcggca tggcgacgtt ctcgagcgcc gtgaactcgg gcagcaggtg
     1201 atggaactgg tacacgaagc cgagcgcgcg attgcgcagg gtgccgcgcg cggcgtcgga
     1261 cagctcggcg agcgactggc cggcgatgct gaccgtgccg gtcgtcggca ggtcgaggcc
     1321 accgaggatc tgcagcaacg tcgtcttgcc cgaccccgac gcgccgacga tcgcgaccgt
     1381 ttcgccacgg tcgatggcga agtccacacc cgccagcacc acgaggcgct ggccgccttc
     1441 gtcgaagtgc ttgctcactc cccggcagct caggatctcc gcgtcagtca tgtcgcaatg
     1501 ccctcgccgg gttggtgcgc gccgcgcgcc acgcgggata cagggtggaa aggcagcaca
     1561 gcgcgaaggc ggtgccggcg atcaggacca catcgccggc ctcgacgcgt gccggcaggt
     1621 cgctcatgag gtagacgctc gcatccatga actgcgtgcc cagcagccgc tcgagtccgt
     1681 gcacgagcgc ctccaggttg acggacagca ataccccgag cgccaccccg cccagcgtgc
     1741 cgaggaaccc gatgaccgtc ccctgggtcg cgaagatccc gagcacgctc gcaggcttcg
     1801 cgccgatggt gcgcaggatc gcgatgtcgg gttgcttgtc cttcaccgcc atcacgagtg
     1861 tcgagacgat gttgaacgcc gccaccgcga cgacgagcag caggatgaag aacatcatgc
     1921 gcttggtgag ctcgatggaa cggaagaagt tcgcctggcg ttgcgtccag tcgtccaccc
     1981 acagcccgcc gccgagggca cgcgcgacgt cgcgggacac gaccggcgcc tgataggggt
     2041 cgcgcatcgc gagccgcagt cccgtgacgc ggtcgccgag ccggaacatg cgctgcgcgt
     2101 ccgcaaggct cgtgagcgcc agtccattgt cgatctcgta cattcccgag cggacgatgc
     2161 cggccaccgt gtagcggcgc atgcgcggca cgacgccggc cggcgtcacc gagccttgtg
     2221 caatcgccat cacgacgctg tcacccggcg cgacgccgag atgggacgcg agttcggacc
     2281 cgagcagcat ccggtacgaa cccggccgca gggcgtccag tgacccgctc agcagccggt
     2341 cggcgatcgc cgacaccgtc gcctcgagct gcggatccac gccacgcacc gtcgccggtg
     2401 ccgcggcgcc ctgcggacga tcggcgatga gcagcgcctc gccctccacg tacggcgcgg
     2461 ccgccgcgac ccgcgggtcc ttcagcgccg tccggcgcat gccgggccag tcctcggccc
     2521 cggccgcgaa tgcgctcagc gtcgcgtgcg aggtgatgct caggatgcgg ctgcgcagct
     2581 cggtctcgaa accgttcatg accgagagca cgacgatcag cacggccacg ccgatcgtga
     2641 cgccgagcat ggacatgagc gagatgaacg acaggaagcg attgccggac gcgcgcaggt
     2701 agcgccgccc gatccggatc tcgaaaggcg tcgcggcgct catcattcgt accgcaacgc
     2761 ttccgcaggc tcggtcgccg caccgcgcat ggccgggtag atcgtggcca ggacggtgag
     2821 cgcgaacgcg attccggcga tgaccatgac ctgcgctgcg cgcagctcgg agggcagctc
     2881 ggtgatgtag tacacggtcg ggtccatgac gtggaacccg aacgtgcgct cgagcgccgg
     2941 tacgatgaca ccgacgttgg ccgcgaccag cacgcccagc aaggtcccga ggacggtgcc
     3001 gatcgcgccg atcacgatgc cctgggtgag gaagatcccg accaccccgc gcggcgcgag
     3061 gcccagcgtg cgcaggatgg cgatgtccga gcgcttctcg ttcacgacca tcaccagtgc
     3121 cgcgacgatg ttgaatgcgg ccaccgcgac gacgagcatg aggatcaggc tcatcatcgt
     3181 cttctcgatg cggatcgcgc ggaaatacgc ggcgtgctcc tggctccagt cgcggacccg
     3241 gaaaccgtcg cccagagctg cggcgacggc ggcggcccgg cgcggcgcgg ccatgacgtc
     3301 gtcgaaacgc agccggatgc ccgcgggcgt tgcgccgctg cccgcgaggc tcgcggcatc
     3361 ggccatcgcc accagcgcca gcttcgaatc gcggtcctgc aggccgactt cgaagatccc
     3421 gcagacctcg aacgtctgca gcgtcgggcg cccggcgccg gtgagcaggt ctcgcccggg
     3481 caccatcacg gtgagctggt ctcccacgcg cgcaccgagc tgccatgcca gcgcgcgacc
     3541 gacgattgcg cagcgcgatc ccggcgtgag gtccgcgagc cgcccttcga gcaggtgttc
     3601 ctccacgctc gaaacgcccg gctccgcccg gtagtcgacg ccgtgcagga cgaccggcag
     3661 cagctgcccg gaccgccctg ccattgccag cacgtcgacg aatggcgccg cgcccagcgt
     3721 gcccggcacg gcacgcgcgc gctccgcgac gtgtcgccag tccgccatcg cggccggcgg
     3781 cccggtcacg gtggcatgcg ccgtcaacgc caccaggcga ccccgcaatt cgccctcgaa
     3841 gccgttcatc accgagatga tggtgatcag ggccgccacg cccaggcaga cgccgagcat
     3901 ggagacccac gaaatgaacg acacgaagaa gccctggcgc cgggtccgga cgtagcgcag
     3961 tccgacgtac atgggcaggg gatggaacat gcttaggcct ggcgaaaggc cgcaaggcta
     4021 accgccactg cccggttttg cacgctgcat cacggaccgg ccccggccgg accgccgtgc
     4081 aatccggaat cgcgtcacgc tccggggctc gggtgcggtg ctatagtccg gtcagtcttc
     4141 catcggggac cgtgaacatg cgcaagaccg tgctggccgt atgtgcgatg tcgctgggtc
     4201 tgggcctcgg caccgcgtgc gctcgtgccg accagcttgc attgggtccg tcctcgcaga
     4261 ctgtggccag cgtgcccgat cgtgggctga gcatggccgc cgtcgaaaag cgctacggag
     4321 ccccctccga ccgcttggca gccatcggca agccgccgat cacccgctgg gtctatccgt
     4381 cgttcgtcgt gtacttcgaa tacaacctcg tcatccactc ggtcgcgacc cagcccgccg
     4441 ccgccacgaa ctgatcggcc cgcgcacccc gctggtctgc taatcttcgc cgcccggcgg
     4501 ggatgccccg cccctgcttc gtttagagcg gatcttagga ttcgttcgta agctattgtt
     4561 ttgacaaaca taggtcagcc cgagctaccc gacgcaagcc ggcccgcact gaggtggacc
     4621 ggactggcgg gcgcgggtga ggcgctgcac gttgccgtgg cggcagccgc cctgcgatca
     4681 cccatgctgc tcgtcgcgcg ggacgccgcc agcgcgacac ggctcgagga ggaactgcgc
     4741 tttttcgtgc ctgcgggact gtcggtgctg gcgtttcccg gttacgagac cctgccctac
     4801 gaccagttct ccccgcaccc ggacatcatt tcgcagcggc tgcgcacgct gtcgcgcctg
     4861 ccgtcgctcg gtgccgggat cgtcgtcacc gacgtgccga cgaccctgca tcgcctgcca
     4921 ccgcgttcgt tcgtggacgg ccacgccatc gtcatcgagg tcggccagga actcgatctc
     4981 ggcgcattcc ggctgcgcct cgccgccgcc ggctacgcca gcgtcccgca ggtcggcgcc
     5041 ccgggcgaat tcgccattcg cggctcgctg ttcgacgtgt tcccgatggg cgccgacgcg
     5101 ccgctgcgca tcgacttgct cgaccgccag gtggacagcg tgcggagctt cgacccggac
     5161 acgcagcggt ccggcgaccg cctgccacgt atcgagttgc tgccggcacg cgagttcggg
     5221 ctcggcccgg actcgacccg cgatttccgg cgtcgctacc gtacgcgttt cgagggcgac
     5281 ctcacgcgca tgacgctcta ccgcgacgtg ggcgacggcc acgccccggc cggcatcgag
     5341 tacttcctgc cactgttctt cgacgccacg gcgagcctgt tcgactacct tccgccaggc
     5401 gccgtcgtgg cgctcccgtc cgatcatcga cggctgctcg atgcggcctg ggcgtcggtc
     5461 gaggagcgct acgaggaacg tcgctacgac atcgagcacc cggtgctcgc gccagcggag
     5521 gcttttctgt ctcctgcgga gtggtcggac gccgccacgc gctcgccacg actgctgctc
     5581 ggcgacaccg tcgacgaagg cgagacggtc gccgtacctg ccgtcgacct cggcacgacc
     5641 gaggctccac cgctcaagat cgaccagcgg cgggacgagt cgatcaggga ggttgctgct
     5701 cgcctcgcgg cgcatccggg gcgcgtgctg ctcgcggcgg agtcgcccgg gcggcgcgaa
     5761 ctgctgctgg aactgctgcg gcctcacggc acgccggtgc gagtcgtatc cggctggcag
     5821 gagttcatcg ccggcgacgc gcccctggcc ctgacggttg cgccgctggc gtcgggtgtc
     5881 gcgattccga cggcgcacat gaccgtctat gccgaagagc aactcttcgg cgagagggcg
     5941 cgccaggagc gcaggcggcg acgatcggac cgggaccccg cccggatcat ccagcagctc
     6001 gcggacctgc ggccaggcgc gccggtggtg cacgaggact atggcgtcgg gcgctacctg
     6061 ggcctcgcga ccatggacgc cggcggcgtc gcgtcggaat tcctcgtgct cgaatatgcc
     6121 gacggcgaca agctctacgt gcccgtccag gcgctcgagc gcgtgagccg gtacacgggc
     6181 gccccggcgg aatcggcgcc tctgcacaag ctcggcgggg accagtgggc gaaggcacgc
     6241 agtcgcgccg cggcgcgcat ccgcgatgcg gccgcggaac tgctcgacgt gtatgcgcgg
     6301 cgcgcggcgc gccaggggca ctcgttcgag ttcgacgagt cgcagctcca ggcattcgct
     6361 gccggctttc ccttcgagga aacggctgac caggccgcgg cgatcaacca ggtgctggag
     6421 gacctgcggt cggcgaagcc gatggaccgg gtcgtgtgtg gcgacgtcgg cttcggcaag
     6481 accgaggtgg cgctgcgcgc cgcgttcgtc gccgtgcagg gcggcaagca ggtcgcggtg
     6541 ctcgtgccga cgacgctgct ggcccagcag cactaccaga cgttcgccga ccggttcgcc
     6601 gactggccaa tgcgcgtcga gctgctgtcg cgctttcgcg gcgacgcgca ggcgaagtcc
     6661 gcgctcgtcg gcctggccga gggacgcgtg gacatcgtga tcggcacgca ccgcctgctg
     6721 cagccgggcg tgcgcttcaa ggacctcggg ctcctgatca tcgacgagga gcaccgcttc
     6781 g
//
LOCUS       DAOMFD010000104         6781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4005_length_6781_cov_27.570473, whole genome shotgun sequence.
ACCESSION   DAOMFD010000104 DAOMFD010000000
VERSION     DAOMFD010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6781
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4005_length_6781_cov_27.570473"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="PL152_07125"
     CDS             complement(<1..358)
                     /locus_tag="PL152_07125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49091.1"
                     /translation="MTLVGRRGLTATLVALALLTVAACVLMDAPAWARIVAAAAAALV
                     YAALTRIVRRAVADRDRVITAVADGVAGLRDGDYSVSIAMPRNDAPLRRLVENYNGIG
                     TRLRAQRQDLYQRELLL"
     gene            complement(355..1734)
                     /locus_tag="PL152_07130"
     CDS             complement(355..1734)
                     /locus_tag="PL152_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQR49092.1"
                     /translation="MDIAALEPAVIRGRTILVVDDSEAVRTALEVLLCLEGARVECAA
                     TPAAALERLARGGIDLVIQDMNFSREATSGEEGVALFRAIRRLQPDLPVVLLTAWTHL
                     ETAVELVKAGAADYLAKPWDNDRLVTTARNLLQLRVAMLESRERAWERAESRAGLAAR
                     FDLRGIVYASDAMHAAVQMATQVARADIPVLITGPNGAGKEVIAEIIQANSSVRDGPF
                     VRVNAGALPADLLESELFGAEAGAYTGAKARAGRFEAADGGTLFLDEIGNLPFAGQAK
                     LLRVLQTGEFERLGSTQTRRVSVRVLSATNASLADAIREGRFREDLYYRLNVIEIRVP
                     PLAERRDDVLPLAQHFLEPGFEFTPDAERALLRHGWPGNVRELANCVRRACLLADERT
                     IRPGHLMLPAVSPEAMVRDPDRIEIELALARAGGVVARAARDLGLTRQSLYRRMERLG
                     IASEEPRAS"
     gene            complement(1779..2984)
                     /locus_tag="PL152_07135"
     CDS             complement(1779..2984)
                     /locus_tag="PL152_07135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR49093.1"
                     /translation="MELRPILSAMRRSPTGAILVALQIALALAITVNAFYIISQRLEH
                     MGRDPGLDVANTFVVVFYAKGRDFDAGPAMREDLAILRGLPGVVAATTINAVPLSGGG
                     SGTRYYAEPGEKGKWAQVNYFEVDEQGVDTLGLQLVEGRNFDASVVTSANRNSSAFVP
                     EVILTRAVANELFPGEHAVGKTVYDNLGQPARVVGVVDHMIGSWPSWDKAGQVTLHPV
                     IADEQQARYLVRAKPGQRDEMMKLAEDRLSAIDNGRIVTKVRSLEYVAAGTYADDRAM
                     AFYMGLVVVLLLAVAALGVFGLAAFNVGTRTKQIGTRRAVGARKRDVVRHFLAENWIV
                     TTVGIAAGCVLALALGYWLSTTFELPRLPLYYLVAGVAILWAVGLIAALVPARRAALV
                     SPAVATRSV"
     gene            complement(2993..4321)
                     /locus_tag="PL152_07140"
     CDS             complement(2993..4321)
                     /locus_tag="PL152_07140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR49094.1"
                     /translation="MIAYDFQLALASLRRNPGLSALMVLAVALGIAVCAVTFTIYHAM
                     ATNPIEWKSDQLYEVTIDSWSPDEPADTDQPDMPPPLLTYRDAMAMKDAGIARHNTVM
                     YKSGAVLDAGRTGTKPFAASLRLTTGDFFPMFDVPFQYGGPWDAEADRAGDPVVVLSH
                     ESNQKAFGGENSVGRTVRIANVEFRVTGVLEPWSPSPKFYDLNNGSFDEAEDAYLPFA
                     FGTAHELPVYGNTNCWKSEKIESARDFLNSECVWVQMWTELPTAAERQRYQAALDNYA
                     RAQKAAGRLPRPLNNRLFDVEQWLDFNHVVKKDNKVLVGIALLFLGVCLVNVVGLLLA
                     KFLNGAGLTGLRRALGASRKDIVRQHLAEVMLVGTGGGLLGLLLAVAGLAGIRTIYEP
                     NHDAYDRLTSIDPTVVLATLGLSLAAGLVAGLYPAWRIGRTAPAVYLKTQ"
     gene            complement(4332..5057)
                     /locus_tag="PL152_07145"
     CDS             complement(4332..5057)
                     /locus_tag="PL152_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR49095.1"
                     /translation="MAMLEMRNVTKVYRTDLLETHALREFGLDVEKGEFVAVTGPSGS
                     GKTTFLNVAGLLEAFDGGTYRLDGDDVGGLDDDARSRVRNRKIGFIFQSFNLIPDLDV
                     YDNVDVPLRYRRMPAAERRERIGAALDLVGLSSRTKHLPSQLSGGQQQRVAIARALAG
                     DPALLLADEPTGNLDSLMARQVLDLLEQINDMGTTIVMVTHDPELARRAHRNVQVIDG
                     QITDFKPYEPVPVEARLEATAYA"
     gene            complement(5083..6402)
                     /locus_tag="PL152_07150"
     CDS             complement(5083..6402)
                     /locus_tag="PL152_07150"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR49096.1"
                     /translation="MPLASISKIHRGDASGMRSTEAQDRAVEPGRRWLRDRRVWWASG
                     VLFVVLLAAGAWGIRGWATSDATVARERVRIATVTRGKFVNDVAAQATVVAAVSPELH
                     APAAGTVTLSVKAGDAVKKGGVLATVDSPALRNEYERERATLESLTVDLKREEIDVRR
                     RILQSQQERDMAAVAIRAAEREFARSQLAWDQKVISERDYRRAKDELDEAKLTHDHAV
                     QASALDKESLEFELQTRRLQRERQQLVVADLERRVGELAVRSPVDGMVGSVAVTDKAA
                     VQQDAVLLNVVDLSALEIEFRVAESYAGSLGTGMPAEISYGGKTYAGEVAALSPEVRQ
                     GEVTGRVRFKGEPPAGLRQNQRVSVRIVMDERDGVLKVERGGFYEAGGGRLAYVVRDG
                     VAERAPIQTGAASIREIEITGGLAEGDQVVVSDTEPFKNAARVLLPN"
BASE COUNT          985 a         2388 c         2342 g         1066 t
ORIGIN      
        1 ccagcagcag ctcgcgctgg tagaggtcct gccgctgcgc ccgcaggcgc gtgccaatgc
       61 cgttgtaatt ctcgacgaga cgacgcagcg gcgcatcgtt ccggggcatg gcgatgctta
      121 cgctgtagtc gccgtcgcgc agcccggcca cgccgtccgc aaccgccgtg atgacgcggt
      181 cgcgatcggc caccgcgcgc cgcacgatgc gggtgagcgc ggcgtacacg agggcagcgg
      241 cagccgccgc aacgatgcga gcccacgccg gcgcgtccat gagcacgcac gcggccaccg
      301 tgagcagggc gagcgccacg agcgtggccg tgaggccgcg tcgcccgacc agcgtcatga
      361 cgcccgcggc tcctcgctcg cgatgccgag ccgctccatc cggcgataca gtgactgtcg
      421 cgtcaaccca aggtcacgag ccgcccgggc cacgactccc ccggcgcgcg cgagcgccag
      481 ctcgatctcg atccggtccg gatcgcgaac catggcctcg ggtgaaaccg ctggcagcat
      541 caggtgccct ggccggatgg tgcgctcgtc ggcgagcaag caggcgcgcc gaacgcaatt
      601 ggccagctcc cggacattgc ccggccagcc gtgtcgcagc agcgcgcgct cggcgtcggg
      661 cgtgaactcg aagcccggct cgaggaagtg ctgcgcgagc ggcagtacgt cgtcgcgccg
      721 ctccgcgagc ggcggcacgc ggatctcgat gacgttcagg cggtagtaga ggtcctcgcg
      781 gaagcggccc tcgcggatcg catcagccag tgacgcattc gtcgcggaaa gaacccgcac
      841 cgacaccctt cgcgtctgcg tcgagccgag ccgctcgaac tcgccggtct gcagcacgcg
      901 cagcaacttc gcctgcccgg cgaacggcag gttgccgatc tcgtcgagga acagcgtgcc
      961 gccgtcggct gcctcgaatc gccccgcccg cgccttcgcg cccgtgtacg ccccggcctc
     1021 cgcaccgaac agctcgcttt cgagcaggtc ggcgggcagc gcgcccgcgt tgacgcgcac
     1081 gaacgggccg tcacgcacgc tggaattggc ctggatgatc tcggcgatca cttccttgcc
     1141 tgctccgttc ggcccggtga tgagcaccgg gatgtcggca cgcgccacct gcgtggccat
     1201 ctgcacggcc gcgtgcatgg cgtcgctcgc gtacacgatg ccacgcaggt cgaagcgcgc
     1261 ggccagcccc gcacgcgact cggcccgttc ccacgcacgc tcgcgggact cgagcatcgc
     1321 gacccgcagc tgcagcaggt tgcgtgcggt cgtgaccagg cggtcgttgt cccagggctt
     1381 cgcgaggtaa tccgccgcgc ccgccttgac gagttccacg gcggtctcga ggtgggtcca
     1441 cgccgtgagc agtacgacgg gcaggtcggg ctgcagcctg cggatcgccc ggaacagcgc
     1501 cacgccctcc tcgcccgacg tcgcctcgcg cgagaaattc atgtcctgga tcacgaggtc
     1561 gatgccgccg cgagcgagac gctcgagcgc cgcggccggc gtggcggcgc actcgacgcg
     1621 cgcaccctcg aggcacagca gcacctcgag cgccgtgcgc acggcctcgc tgtcgtcgac
     1681 gacgagaatg gtgcggccgc ggatcacggc cggctcgagc gctgcgatgt ccatccctcc
     1741 cgcggactcc ggccggtcgc cggcatccgc accttcgatc agaccgagcg cgtggcgacc
     1801 gcgggcgaga ccagcgccgc ccggcgcgcc ggcaccagcg cggcgatgag cccgacggcc
     1861 cacaggatcg ccaccccggc caccagataa tagagcggaa gccgcggcag ttcgaatgtc
     1921 gtgctgagcc agtacccgag cgccagcgcg agcacgcagc cggcggcgat accgacggtc
     1981 gttacgatcc agttctcggc aaggaagtgt cgcaccacgt ctcgcttgcg cgcaccgacc
     2041 gcgcgccgcg tgccgatctg cttggtgcgc gtcccgacgt tgaatgccgc cagcccgaag
     2101 accccgagcg ccgcgacggc cagcagcagc acgacgacga gccccatgta gaaggccatg
     2161 gcgcggtcgt cggcgtaggt cccggcggcc acgtactcga gcgagcgcac cttcgtgacg
     2221 atgcgcccgt tgtcgatcgc cgagagccgg tcctcggcga gcttcatcat ctcgtcacgc
     2281 tggcccggct tcgcgcgcac caggtaccgg gcctgctgct cgtcggcgat gaccgggtgc
     2341 agggtgacct ggccagcctt gtcccacgac ggccaggacc cgatcatgtg gtcgaccacg
     2401 ccgaccacgc gcgccggctg cccgaggttg tcgtagaccg tcttgccgac cgcgtgctca
     2461 cccgggaaca actcgttcgc gacagcgcgc gtcaggatga cttccggcac gaacgccgaa
     2521 ctgttgcggt tcgcactggt cacgacgctg gcgtcgaagt tgcgcccctc gacgagttgc
     2581 agtccgagcg tgtcgacgcc ctgctcgtcg acttcgaagt agttcacctg cgcccacttg
     2641 cccttctcgc ccggctcggc gtagtagcgc gtgccggagc cgccacccga gagcggcacg
     2701 gcattgatgg tcgtggccgc tacgacgccg ggcagcccgc gcaggattgc gaggtcttcg
     2761 cgcatcgcag gtccggcgtc gaagtcgcgc cccttggcat agaagaccac cacgaacgtg
     2821 ttggccacgt cgagcccggg gtcgcgtccc atgtgctcga ggcgctggct gatgatgtag
     2881 aacgcattga cggtgattgc gagcgcgagc gcgatctgca gggcgacgag gatcgccccg
     2941 gtcgggctgc ggcgcatcgc gctcaggatg ggtcggagtt ccatggcggc cctcactggg
     3001 tcttcaggta gacggcaggc gcggtgcggc cgatccgcca cgcggggtac agccccgcga
     3061 cgaggcccgc cgcgagcgac agtcccagcg ttgcgagcac gaccgtcgga tcgatcgagg
     3121 tgagtcgatc gtaggcatcg tggttcggct cgtagatggt ccggattccg gccagccccg
     3181 ccactgcgag cagcagcccg agcagcccgc cgccggtccc gacgagcatg acctccgcga
     3241 gatgctggcg gacgatgtcc ttgcggctgg cgccgagcgc acggcgcagt ccggtgagtc
     3301 cggcgccgtt caggaacttc gcgagcagca atcccacgac gttgaccagg cacacgccga
     3361 ggaacagcag cgcgataccg accagcacct tgttgtcctt cttgaccacg tggttgaagt
     3421 cgagccactg ctcgacgtcg aacaggcggt tgttgagcgg gcgcggcaat cggccggccg
     3481 ccttctgcgc ccgcgcgtag ttgtcgagcg ctgcctggta gcgctggcgc tcggccgcgg
     3541 tcggcagctc cgtccacatc tgcacccaga cgcactcgga attgaggaag tcgcgggccg
     3601 attcgatctt ctcgctcttc cagcagttgg tattgccgta gacgggcagc tcgtgcgccg
     3661 tgccgaatgc gaacggcagg taggcgtcct cggcctcgtc gaacgagccg ttgttgagat
     3721 cgtagaactt cgggctcggc gaccacggct cgagcacgcc cgtcacccgg aactccacgt
     3781 tggcgatgcg caccgtgcgg ccgacgctgt tctcgccacc gaacgccttc tggttggact
     3841 cgtgactcag cacgacgacc gggtcgcccg cacggtccgc ctccgcgtcc caggggccgc
     3901 cgtactggaa cggcacgtcg aacatcggga agaaatcgcc ggtggtcagg cgcagcgacg
     3961 ccgcgaacgg cttcgtgccg gtccgtcccg cgtcgagcac cgcgccggac ttgtacatga
     4021 cggtgttgtg gcgcgcgatt ccggcatcct tcatcgccat cgcgtcgcgg taggtgagca
     4081 ggggcggcgg catgtcgggc tggtcggtgt cggcgggctc gtcgggcgac cagctgtcga
     4141 tcgtgacctc gtagagctgg tcgctcttcc actcgatcgg gttggtcgcc atcgcgtggt
     4201 agatggtgaa cgtcacggcg cacacggcga tgccgagcgc cacggcgagc accatcagcg
     4261 cggacaggcc cgggttgcgc ctgaggctcg cgagcgcgag ctggaaatcg taggcgatca
     4321 tggcggcgtc ctcaggcgta ggccgtcgcc tcgagtcgcg cctcgacggg cacgggctcg
     4381 tagggcttga agtcggtgat ctggccgtcg atgacctgca cgttgcggtg tgcgcgccgc
     4441 gcgagttccg ggtcgtgcgt gaccatgacg atggtcgtgc ccatgtcgtt gatctgctcg
     4501 agcaggtcga ggacctggcg cgccatgagc gagtcgaggt tgccggtcgg ctcgtcggcg
     4561 agcagcagcg ccgggtcgcc cgcgagcgcg cgtgcgatcg ccacgcgctg ctgctgtccg
     4621 ccggacagtt gcgacggcag gtgcttcgtc ctggacgaca gcccgaccag gtcgagcgcg
     4681 gcgccgatcc gctcgcgtcg ctcggccgcg ggcatgcgcc ggtagcgcag cggcacgtcg
     4741 acattgtcgt acacgtcgag gtcgggaatc aggttgaagc tctggaagat gaagccgatc
     4801 ttgcggttgc ggacccgcga gcgggcgtcg tcgtcgaggc cgccgacgtc gtcgccgtcg
     4861 aggcggtagg tgccgccgtc gaaggcttcg agcagcccgg ccacgttgag gaaggtcgtc
     4921 ttgcccgacc ccgatggacc ggtcaccgcg acgaattcgc ccttctccac gtcgagcccg
     4981 aattcgcgca gcgcgtgcgt ctcgagcagg tcggtccggt agaccttggt gacgttgcgc
     5041 atttccagca tggccatggg gtttcctcgt gggtaggcgt ggtcagttgg ggagcagcac
     5101 ccgggctgca ttcttgaatg gctcggtgtc ggacacgacg acctggtcgc cctcggcgag
     5161 cccgccggtg atctcgatct cgcggatgct cgcggcaccg gtctggatgg gcgcgcgctc
     5221 agcgacacca tcgcgcacga cgtaggccag ccggccgccg ccggcttcgt agaagccgcc
     5281 gcgctcgacc ttcagcacgc cgtcgcgctc gtccatgacg atgcgcaccg agacgcgctg
     5341 gttctgtcgc agcccggcgg gcggctcgcc cttgaatcgc acgcgacccg tcacctcgcc
     5401 ctggcgcact tccggcgaca gggcggcgac ctcgcccgcg taggtcttgc ccccgtagct
     5461 gatctcggcc ggcatgccgg tgccgaggga tcccgcgtac gactcggcga cccggaactc
     5521 gatctcgagc gcggataggt ccacgacgtt gagcagcacc gcgtcctgct ggacggccgc
     5581 cttgtccgtg accgccacgc tgccgaccat gccgtccacc ggcgagcgca ccgcgagttc
     5641 gccgacgcgc cgctccaggt cggcgaccac cagctgctgg cgctcgcgct gcaggcgacg
     5701 cgtctgcagc tcgaactcga ggctttcctt gtcgagcgcc gaggcctgca ccgcatggtc
     5761 gtgggtgagt ttcgcttcgt cgagttcgtc cttcgcgcgc cggtagtcgc gctcgctgat
     5821 cactttctgg tcccaggcca gctgcgagcg ggcgaactcg cgctcggcgg cgcggatcgc
     5881 cacggccgcc atgtcgcgct cctgctggct ctgcaggatg cggcggcgca cgtcgatctc
     5941 ctcgcgcttc aggtccaccg tcaggctctc gagcgtcgcg cgctctcgct cgtactcgtt
     6001 cctcagcgcg ggactgtcca ccgtcgcgag gacgccgccc ttcttgacgg cgtcacccgc
     6061 cttcactgac agggtcacgg tgcccgccgc cggtgcgtgc agctcgggac tcacggcagc
     6121 gaccaccgtc gcctgcgccg ccacgtcgtt cacgaacttg ccgcgcgtga ccgtcgcgat
     6181 gcgcactcgc tcgcgcgcga ccgtcgcatc ggaggttgcc caaccgcgaa tcccccaggc
     6241 gccggcggcc agtagcacga cgaacaagac accgctggcc caccagaccc gtcggtcgcg
     6301 cagccagcgt cggcccggct cgaccgcgcg gtcctgcgcc tccgtgctgc gcatgccgga
     6361 cgcgtcgccg cggtggatct tggaaatact ggccaggggc atggatcact cggttgcact
     6421 aggtccctgg atgtcattgc acttcttgtg ccagtcgccc aggacttgcg caggcacatg
     6481 attcttcgac ggtttgctgg gcacaaaggt ttgtatcgat ggtggtgctg gtgtccgcgg
     6541 ccggacggcg gacacttttg cggacacttt cgcgccctcg cgggccgtgc ggaccgatag
     6601 cggttaaagt accgaggccc gcgtcggcaa ggcgaccggc gcggcgggga gaacggcggg
     6661 atggccgacg tgttcgtgtc gtattcgcgc ggcgacaagg cccgggtggc cccgctcgtg
     6721 gcggcgctcg agggccaggg ctgcacggtg tggtgggatc cggccatcac gccgggacag
     6781 g
//
LOCUS       DAOMFD010000105         6771 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4019_length_6771_cov_31.405450, whole genome shotgun sequence.
ACCESSION   DAOMFD010000105 DAOMFD010000000
VERSION     DAOMFD010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6771)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6771
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4019_length_6771_cov_31.405450"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..823)
                     /gene="secD"
                     /locus_tag="PL152_07155"
     CDS             complement(<1..823)
                     /gene="secD"
                     /locus_tag="PL152_07155"
                     /inference="COORDINATES: protein motif:HMM:NF025098.2"
                     /note="part of the preprotein secretory system; when
                     complexed with proteins SecF and YajC, SecDFyajC
                     stimulates the proton motive force-driven protein
                     translocation, and appears to be required for the release
                     of mature proteins from the extracytoplasmic side of the
                     membrane; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HQR49097.1"
                     /translation="MHSYPAWKIWLVTIVMLVALLLALPNVFGEAPALQLSRNDRTAF
                     NDQSQATVTGILSAKGVPAEAAYREGDRLVLRFESVDRQLKARDVIREAAPRDYLIAL
                     SQVPRTPAWMRAIGLKPMSLGLDLRGGVYFMYEVDVKGAVAQLLASMERDYRTLLRDE
                     RIPYSGMSNDGVDSVKILLRTGADAPRVASALRKRDPNLLIETDTLGEGGAVTVTLRP
                     ERIKERQDFAIQQNITTLRNRVDELGVTEPIVARQGVDRIVVQLPGVQDPNEALRV"
     gene            complement(840..1169)
                     /gene="yajC"
                     /locus_tag="PL152_07160"
     CDS             complement(840..1169)
                     /gene="yajC"
                     /locus_tag="PL152_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HQR49098.1"
                     /translation="MDWLIPTAYAQAAGGAQPNAFMSMLPLVLIFVVFYFLLIRPQTK
                     RAKEHKAMVAALAAGDEIVTSGGILGRITEAGEQFLTVEVAEGVRVKIQRHTVSSVLP
                     KGTLKSA"
     gene            complement(1226..2374)
                     /gene="tgt"
                     /locus_tag="PL152_07165"
     CDS             complement(1226..2374)
                     /gene="tgt"
                     /locus_tag="PL152_07165"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HQR49099.1"
                     /translation="MSARFELFATSGRARRGRLALRRGTVETPVFMPVGTYGTVKAMT
                     PEELAGLGAEIVLGNTFHLMLRPGTDVIRAHGGLHGFMHWPGPILTDSGGFQVFSLSE
                     LRKLDEDGVRFRSPVNGDPVFLSPEISMSVQTALDSDVAMAFDECPPYPATEPEARRS
                     MELSMRWARRSRHAFQSLANPNALFGIVQGGTHLPLRIESAGILRDIGFDGYAIGGLA
                     VGEPAAERNSVLEALEPHLPADRPRYLMGVGTPADIVQAVSRGVDMFDCVMPTRNARN
                     GHLFTSAGVVKIRNAAHHDDTRPLDPECGCYTCRNYSRAYLRHLDRCNEILGARLNTI
                     HNLHYYLALMERIRSAIGQGRFEAFAAAFLAGPEGGIVPGGSPRDPRV"
     gene            complement(2371..3414)
                     /gene="queA"
                     /locus_tag="PL152_07170"
     CDS             complement(2371..3414)
                     /gene="queA"
                     /locus_tag="PL152_07170"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HQR49100.1"
                     /translation="MLRAEFHYELPDELIARHPTARRSDSRLLRLDGSNGRVADHNVA
                     DLPRLLRAGDLMVFNDTRVVPARMFGRKASGGRVEMLLERVLGNKRALVQLRSSKPPA
                     SDSAIHLPGDVEARVAGRSGEFWLVEFDSDPGAVFERHGEMPLPPYLRRPVEDDDRER
                     YQTVYAREPGAVAAPTAGLHFDEGLLAACREAGIHSARVTLHVGAGTFQPVRVDDVRE
                     HRMHAERVDVPDATCAAVRACRERGGRVVAIGTTSVRALESAASGGALAPFQGDTRLF
                     ITPGFRFRVVDALVTNFHLPESTLLMLVCAFAGREHVLAAYRHAVAARYRFFSYGDAM
                     LVEPDPAALEARA"
     gene            complement(3473..4471)
                     /locus_tag="PL152_07175"
     CDS             complement(3473..4471)
                     /locus_tag="PL152_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HQR49101.1"
                     /translation="MLTRNLVQRRWIGTLAVALSICAPVAMAADAPPDQAALQKQLDE
                     ARTRLDAAARDVARLSTELYGDRDAQFARMMHGPQGAMLGVNIGGSRGLDTGVEVAGV
                     SPGGPAEAAGLRAGDVIVAVDATQLKQADGRSPSAQLVGYMRSVEPGKTVRVQYLRDG
                     KRLTADVKTIAAEPMFFGLMRDHMPRGPLGMGPGGMSHPGFHGMLMGRPGFGSLELVP
                     MTPGLGRYFGTDKGLLVVRAPREAGVGLEDGDVLMTIGGRTPDSAGQAFRILQSYDVG
                     DKVQLGILRNRKRMDVDATMPTPAAGRPVGPPPPSPAEPPRPAAPPPPADGKEGSA"
     gene            complement(4494..5123)
                     /locus_tag="PL152_07180"
     CDS             complement(4494..5123)
                     /locus_tag="PL152_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49102.1"
                     /translation="MHARIDQLLSIRDGEPVDAAVQRHVESCHTCGSEIARLAAVRDG
                     LGVLAAAEARPGEWMAVQARITRRARAIRQGALAWRAVAAATFIALVLSLVASFDREP
                     DGGVTLATNTAAPMHTSSGDVASLVERSRQLEQVLASMPSRPAVERADTSMPIDALEA
                     RVRWLDHQLSQGDGLALPGGGAERLWRERVDIMNSLVRLRYVEAQRVAL"
     gene            complement(5096..5689)
                     /locus_tag="PL152_07185"
     CDS             complement(5096..5689)
                     /locus_tag="PL152_07185"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HQR49103.1"
                     /translation="MTPFRDSALPPGTLERARSGDPGAHEAIYRVFEVPVRTLARRLV
                     PRRAAADDLAQDVFVEVLTRLSQFDGRGSFAGWVRTITVNRCLMHLRSPWQRARHALD
                     VDGAHAWVDPAAEQAAQDRGRRLDLERALARLGPTPRVVVWLHDVEGYTHAEIGALFG
                     ATASFSKSQLARAHERLRGLLEPDGVASTCMPVSTNC"
     gene            complement(5785..6513)
                     /locus_tag="PL152_07190"
     CDS             complement(5785..6513)
                     /locus_tag="PL152_07190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase catalytic domain
                     family protein"
                     /protein_id="HQR49104.1"
                     /translation="MRRRPWLIAWLLLAGIAPARADDALAAELAAAAPAANPKVLALA
                     TRAAECARQRGMLDAFHRLAVIDYSLPSTLPRLWVFDVDRGRLLFQELVAHGRNTGER
                     IAEHFSNAEGSKMSSLGLFRTADTYYGNNGYSLRLDGLEAGFNDNALARAIVMHGAPY
                     VSEAIADRLGRLGRSWGCPAVRPEVARVMIDTLKGGALLFAYYPDSKWLSESPMLTCA
                     PAPATTAAAADRVAASSRLATPSG"
BASE COUNT         1024 a         2368 c         2373 g         1006 t
ORIGIN      
        1 gcacgcgcag cgcctcgttc gggtcctgca cgcccggcag ctgcacgacg atgcggtcga
       61 cgccctggcg cgcgacgatc ggttcggtga cgccgagctc gtcgacgcgg ttgcgcagcg
      121 tcgtgatgtt ctgctggatc gcgaaatcct gccgttcctt gatgcgctcg ggccgcaggg
      181 tgacggtcac cgcgccgccc tcgccgagcg tgtcggtctc gatcagcaga ttcgggtcgc
      241 gcttgcgcag cgcggaggcg acgcgcggcg cgtccgcccc ggtgcgcagc aggatcttca
      301 cggagtccac gccgtcgttc gacatgccgc tgtacgggat gcgctcgtcg cgcagcagcg
      361 tgcggtagtc gcgctccatg ctggccagca gctgcgccac cgcacccttc acgtcgacct
      421 cgtacatgaa gtagacgccg ccgcgcaggt cgaggcccag gctcatcggc ttcagtccga
      481 tcgcgcgcat ccaggccggc gtgcgcggca cctgcgacag cgcaatcagg tagtcgcgcg
      541 gcgcggcctc gcggatgacg tcacgggcct tgagctggcg atcgacactc tcgaagcgca
      601 gcacgaggcg gtctccctcg cggtatgcgg cctcggccgg gacgcccttg gccgagagta
      661 tcccggttac cgtcgcctgg gactggtcat tgaacgcggt acggtcgttg cgcgacagct
      721 gcagcgcggg ggcctcgccg aacacgttcg gcagcgcgag cagcagggcc accagcatga
      781 cgatggtgac cagccagatc ttccaggccg ggtaactgtg catggtcggc ttccggcgct
      841 caggcgctct tcagggtgcc cttgggcagg acgctcgaga cggtgtgacg ctggatcttg
      901 acgcgcacgc cctccgcgac ctcgaccgtg aggaactgct cgccggcctc ggtgatccgg
      961 ccgaggatgc cgccgctcgt gacgatctcg tccccggccg cgagcgccgc gaccatcgcc
     1021 ttgtgctcct tggcacgctt ggtctgcggg cggatcagca ggaaatagaa gacgacgaag
     1081 atcaggacca gcggcagcat cgacatgaat gcgttcggct gcgcgccgcc agcggcctgc
     1141 gcgtaggcag tcgggatcaa ccaatccatc aggggaactc ctgtgccggg cccgggtgag
     1201 cgggccaccc aaaaaaggtc gtgaattata cccgagggtc gcgcgggctg ccgcccggga
     1261 cgattccgcc ctcgggaccc gccaggaatg cggcggcaaa ggcttcgaac ctgccctgcc
     1321 cgatggccga ccggatgcgc tccatgagcg ccaggtagta atgcaggttg tggatcgtgt
     1381 tgaggcgcgc gccgaggatc tcgttgcagc ggtccaggtg gcgcaggtag gcccgcgaat
     1441 agttgcggca ggtgtagcag ccgcattccg ggtcgagcgg ccgcgtgtcg tcgtggtgcg
     1501 cggcgttgcg gatcttcacc acccccgcgc tcgtgaacag gtggccgttg cgggcgttgc
     1561 gcgtcggcat cacgcaatcg aacatgtcca cgccgcgcga taccgcctgc acgatgtcgg
     1621 ccggcgtgcc cacgcccatc aggtagcgcg gccggtccgc cggcaggtgc ggctcgaggg
     1681 cctccagcac ggagtttcgt tccgccgccg gttcgccgac cgccagtccg ccgatcgcgt
     1741 aaccgtcgaa gccgatgtcg cgcaggatgc ccgcggactc gatgcgcagc ggcaggtggg
     1801 taccaccctg gacgatgccg aagagcgcat tgggattggc gagcgactgg aacgcgtggc
     1861 ggctgcgtcg tgcccaccgc atcgagagtt ccatcgagcg gcgcgcctcg ggctccgtcg
     1921 ccgggtacgg cgggcactcg tcgaaggcca tcgccacgtc cgagtcgagc gccgtctgca
     1981 cgctcatcga gatctcgggg ctcaggaaca ccggatcgcc gttcaccggg gaacggaacc
     2041 gcacgccgtc ctcgtcgagc ttcctgagtt cgctgaggct gaagacctgg aagccgccgg
     2101 agtccgtcag gatcggaccg ggccagtgca tgaacccgtg cagcccgccg tgcgcgcgga
     2161 tcacgtcggt gccgggccgc agcatcaggt ggaaggtgtt gccgagcacg atctccgccc
     2221 cgagacccgc cagctcctcc ggcgtcatgg ccttgaccgt gccgtaagtc ccgaccggca
     2281 tgaacaccgg cgtctcgacg gtgccgcgcc tgagcgcgag gcggccgcgc cgcgcccggc
     2341 cgctggtcgc aaagagctcg aagcgggcgc tcacgcgcgt gcctccagcg cggcggggtc
     2401 gggctcgacc agcatcgcat caccgtagct gaaaaagcgg taacgcgccg cgaccgcgtg
     2461 ccggtaggcc gcgagcacgt gctcgcggcc cgcgaacgcg cagacgagca tgagcagcgt
     2521 ggactccggc aggtggaagt tggtcacgag cgcgtccacg acccggaagc ggaagccggg
     2581 ggtgatgaaa agacgcgtgt ctccctggaa tggagccagg gctccgccgc tggcggccga
     2641 ctcgagcgcg cgcaccgacg tggtcccgat cgcaacgaca cgtccgccgc gctcacggca
     2701 tgcgcgaacg gctgcacacg tcgcgtccgg tacgtcgacc cgttccgcgt gcatgcgatg
     2761 ctcgcggacg tcgtcgaccc ggaccggctg gaacgtcccc gcgccgacgt ggagggtgac
     2821 cctggcgctg tggatgccgg cctcacggca ggccgcaagc agaccctcgt cgaaatgcag
     2881 tccggcggtc ggggccgcca cggcgccggg ctcgcgggcg tagacggtct ggtatcgctc
     2941 ccggtcgtcg tcctccacgg gcctgcgcag gtagggcggc agtggcatct cgccatgcct
     3001 ctcgaagacg gcgccggggt cggagtcgaa ttccacgagc cagaactcgc cggatcgccc
     3061 ggcgacgcga gcctcgacat cgcccgggag gtgaatcgcg gagtcgctcg ccggcggctt
     3121 gctggagcgc agttgcacga gcgcgcgctt gttgccgagg acgcgctcga ggagcatctc
     3181 gacgcgaccg ccgctggcct tgcgtccgaa catgcgggct ggaacgaccc gcgtgtcgtt
     3241 gaacaccatc aggtcgcccg cgcgcagcag acgcggcagg tcggcgacgt tgtggtcagc
     3301 caccctgccg ttacttccgt cgaggcgcag cagtcggctg tcgctgcgcc tcgcggtcgg
     3361 atggcgggcg atcagctcat ccggcaattc gtaatggaac tcggcgcgca gcatggggcg
     3421 cgcatgctag cagagggccg gcgcgcgccg gcccgcggcc agcaacgcgc cgtcaggcag
     3481 acccctcctt gccgtctgcc gggggcggtg gtgctgcggg acgcggcggc tctgctggtg
     3541 aaggtggcgg cggcccgacc gggcgaccgg ccgcgggggt cggcatcgtc gcgtcgacgt
     3601 ccatgcgctt gcgattgcgc aggatgccga gctgaacctt gtcgcccacg tcgtacgact
     3661 gcagtatccg gaaggcctgg cccgcgctgt ccggcgttcg gccgccgatc gtcatgagca
     3721 cgtcgccgtc ctccagcccg acacctgcct cgcgcggcgc ccggaccacg agcagaccct
     3781 tgtccgtgcc gaagtagcgg cccagccccg gggtcatcgg gaccagctcc agcgatccga
     3841 atcccggtcg acccatcagc atgccgtgga accccggatg cgacatgcct cccggtccca
     3901 tgcccagcgg gccgcgcggc atgtgatcgc gcatcagccc gaagaacatc ggttccgccg
     3961 cgatcgtctt cacgtcggcg gtcagtcgct tgccgtcgcg caggtactgc actctcaccg
     4021 tcttgcccgg ctcgacgctg cgcatgtatc ccacgagctg tgccgatggc gagcgtccgt
     4081 cggcctgctt gagctgcgtg gcgtcgacgg cgacgatgac gtcgccagcg cgcagccccg
     4141 ccgcttccgc ggggccgccc ggactcacgc ccgccacctc gacaccggtg tcgagcccgc
     4201 ggcttccgcc gatgttcacc cccagcatcg ccccttgcgg gccgtgcatc atccgtgcga
     4261 actgcgcatc gcggtccccg tagagctcgg tactcagtcg ggccacgtcg cgcgcggcgg
     4321 cgtcgagcct ggttcgcgcc tcgtcgagct gcttctgcag cgccgcctgg tcgggcggag
     4381 cgtcggcagc catcgcaacg ggcgcgcata tcgacaaggc gacggcgagc gtcccgatcc
     4441 atcgtcgctg cacaagattt ctggtcagca tccgtcctgc ctcctgtcgt cgactacagt
     4501 gccacccgct gcgcttcgac gtagcgcagc cgcaccagcg aattcatgat gtccactcgc
     4561 tccctccaca gccgctccgc accgcccccg ggcagtgcaa gcccgtcacc ctgcgacaac
     4621 tggtggtcca gccaccgcac gcgcgcctcg agtgcgtcga tcggcatcga ggtgtcggcc
     4681 cgctccacgg ccgggcggga cggcatcgac gccagcactt gctcgagctg gcgcgagcgc
     4741 tcgaccaggg acgcaacgtc gcccgaggag gtgtgcatcg gcgcagcggt attcgtcgcc
     4801 agggtcacgc caccatccgg ttcgcggtcg aagctggcga cgagcgacaa gacgagcgcg
     4861 atgaaggtcg cggcagccac ggcgcgccag gccagcgcac cctgccgaat cgcacgggcg
     4921 cgccgggtga tgcgcgcctg cactgccatc cattccccgg gtcgtgcctc ggctgccgcc
     4981 agcacgccca ggccatcgcg gacagccgcg agtcttgcga tctcgctgcc gcaggtatgg
     5041 caggactcga cgtgccgctg cacggcggcg tcgacgggct cgccgtcgcg gatgctcagc
     5101 agttggtcga tacgggcatg catgtggatg ccactccgtc gggctcgagc aggccgcgca
     5161 ggcgctcgtg cgcgcgcgcc agctgtgact tggaaaaact cgcggtcgcg ccgaacagcg
     5221 cgccgatctc ggcgtgcgtg tacccctcga cgtcgtgcag ccagaccacg acgcgcggcg
     5281 tgggcccgag gcgcgccagc gcacgctcca ggtcgagccg tcggcctcgg tcctgtgcgg
     5341 cctgttccgc cgctggatcc acccaggcgt gtgcaccatc gacatcgagg gcgtgccggg
     5401 cgcgctgcca tggcgagcgc aggtgcatga ggcagcgatt gacggtgatt gtgcgcaccc
     5461 agccggcgaa cgagccgcgt ccgtcgaact gggagaggcg cgtgaggacc tcgacgaaca
     5521 cgtcctgcgc gaggtcgtct gccgcggcgc gccgcggcac gagccgccgc gcgagcgtgc
     5581 ggaccggcac ttcgaacacc cggtagatcg cctcgtgcgc accgggatcg ccggagcggg
     5641 cgcgctcgag ggtgccgggc ggcagtgcgc tgtcgcgaaa cggggtcatt caccccgtta
     5701 gatgcgccag gccggaaaaa ggtcgcggaa aggcccgtgc gcgcgggggg cacccgcggg
     5761 cgggcggtgg ggctaagccg gtcctcagcc ggaaggagtc gccaggcgcg aacttgcggc
     5821 cacgcggtcc gcagcagcgg cagtggtcgc aggagcggga gcgcaggtca gcatcggcga
     5881 ttcgctcaac cacttgctgt ccgggtagta ggcgaacagc agcgccccgc ccttcagcgt
     5941 gtcgatcatc acgcgcgcga cctccggtcg cacggccggg cagccccagc tgcgcccgag
     6001 ccgtcccagt cggtcggcga tggcctcgct gacgtacggc gcgccgtgca tcacgatcgc
     6061 acgcgcgagc gcgttgtcgt tgaatccagc ttccaggccg tcgagccgca gggaatatcc
     6121 gttgttgccg tagtacgtgt cggccgtccg gaacaggccc aggctcgaca tcttgctgcc
     6181 ctcggcattc gaaaaatgct cggcgatccg ctcacccgta ttgcgaccgt gcgcgacgag
     6241 ttcctggaac agcagccgac cgcggtccac gtcgaagacc cacaggcgcg gcagggtcga
     6301 gggcagggaa tagtcgatca ccgcgaggcg gtgaaaggcg tcgagcatgc cacgttgccg
     6361 cgcacattcc gcggcgcgcg tcgccagtgc aaggaccttg ggattggcgg ccggcgctgc
     6421 agcggccaac tcggccgcga gcgcatcgtc cgcccgggcg ggagcgatac ctgccagcaa
     6481 cagccatgca atcagccagg gacgtcgtcg catgcgagca ctctccgtgc attgagcgcg
     6541 tgccgcccgc aggcgacgtg gtgcagcctc agaccagccg gccgtcgatg ggttcccacg
     6601 ccgtccggac cgcacgcgtc gggaatttga catcactttg catgtcgtcg gcaagtaact
     6661 gacgcacaaa gtgtttaggg tccccgggtg ccgatcgggt aagcttcgcg gtccgtgccg
     6721 ggatggcgga actggtagac gcaccggact caaaatccgg cggtggcgac a
//
LOCUS       DAOMFD010000106         6714 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4091_length_6714_cov_31.030785, whole genome shotgun sequence.
ACCESSION   DAOMFD010000106 DAOMFD010000000
VERSION     DAOMFD010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6714)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6714
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4091_length_6714_cov_31.030785"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(46..2697)
                     /locus_tag="PL152_07195"
     CDS             complement(46..2697)
                     /locus_tag="PL152_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49105.1"
                     /translation="MQAKKAGPGAERARLAASTSRQAGEWKAFGPWLSERAWGTVRED
                     YSADGDAWRYFPHDHARSRAYRWSEDGLAGLCDAGQNLCFALVFWNGRDPILKERIFG
                     LTGHEGNHGEDAKECWWYEDATPTASWLRWRYHYPQAEFPYARLREENARRNRGDREF
                     ELADTGIFDDGRYWAITADYAKASPRDVCMRLTIHNAGPDAAELHVLPTLWFRNRWSW
                     GDDEPRPSIHAAGPDVGNAAIAVVEDSRIGNWSLTAGAPSGGARPTLLFCENETNAPR
                     LFGVPGSTPYPKDGINDHVIHGAATVNPARRGSKMSCWYRLAVPAGQSVELRLRLARQ
                     EHIAEADLGTEFEQVLATREREADEFYAELRPAAATDEEARIMRQAFAGMTWGQQFYH
                     FDVAKWLDGDAVPPPASRLTGRNADWRHLSNHDILAMPDKWEYPWFAAWDLAFHCIVL
                     AHTDPEAAKHQLLLLGREWYMHPNGQLPAYEWNFGDVNPPVQAWAALRVFAIDGSRDL
                     EFLARAFQKLLINFTWWVNRKDVLGDNIFEGGFLGLDNIGAFDRSTPLPEGWILEQSD
                     GTAWMAKYCLNMLEIALRLANHDRAYEDVALKFFEHFCSIARATRELWSEDDGFFYDR
                     LRKPDGTTIPLRARSMVGLLPLFAAVRIDPRLWEQLSDFRARARWYIDHNPWVGEFLH
                     FDPDDRRPALLTLVDHDRLVRVLARMLDEQEFLSPFGLRSLSRYHRDHPLVVPLPGGE
                     ARLDYEPAESTSGLFGGNSNWRGPVWFPFNYLALESLRHLHECFGADFTVELPTGSGV
                     HVRLCDVADELERRLLSLFVRGADGRRPSDDAPASFRRDPAWRDPMLFHEYFHGDTGA
                     GLGASHQTGWTALAGALIADRRRRKVT"
     gene            complement(2731..3045)
                     /locus_tag="PL152_07200"
     CDS             complement(2731..3045)
                     /locus_tag="PL152_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49106.1"
                     /translation="MRHRIVIAAACLAGLAGCESTDQQLDAQQQQAVQTAVQRVSFDM
                     NCPSATGQVLSREMVAPVSIRFGVYRAEYTVGVEGCGQRQTIVVVCPQDGSGCFAGGA
                     RE"
     gene            complement(3099..4544)
                     /locus_tag="PL152_07205"
     CDS             complement(3099..4544)
                     /locus_tag="PL152_07205"
                     /inference="COORDINATES: protein motif:HMM:TIGR01845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HQR49107.1"
                     /translation="MRARTAIILVSLALPACMVGPDYHRPEVKSPDAFRFEPQQATAD
                     ADAEWWKQFGDPVLDALIAEALANNWNVRIATANVEQAAGVLTTTRSPLFPQVGYSGV
                     AARDKFSTHGTTDLASGIENPTDAYQVLAGATWEIDLWGRIRRLTEAAQADLLATQEA
                     RRGVILSLVATVATGYLQLRGLDEQLTMAERTQATYAESLRVMELKFKYGRVSQMNVE
                     QARARYQTATAQIPRIRRDIAVLENGLSIALGRNPGPIERGKSIFDITLPQVPAGVPS
                     DLLQRRPDVMQAEQLLVAANAQIGAAEAQFFPTISLTGALGSASTELSDLFKGPAGTW
                     SYAGQVAGPIFSGGAIYGQVKQAKAAEQAALASYQLAIKNAFADVDNALVSRTTLAEQ
                     VAAQEKLVDALRGYQHMAQLQFDVGRAPYATVLQAEEQLFPQELAWAAARADLCASLA
                     GIYKAMGGGWVAKAADMTESSAAAGGAAKSP"
     gene            complement(4546..>6714)
                     /locus_tag="PL152_07210"
     CDS             complement(4546..>6714)
                     /locus_tag="PL152_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49108.1"
                     /translation="VVLVVLVVFVFLHSMRLTIIPTLAVPVSILGALVGMLLFGFSIN
                     MLTLFGMILAIGLVVDDAIVVVENTERNMVQFNLSAKEAAKRAMDEVSGPVIAVVLVL
                     NAVFIPVAFLGGITGALYKQFAVTIAISVVFSGLVALTLSPALAAILIQARHEEKKGF
                     FKWFDDTLTSLTDSYVGGVKWVTHNWVRGLGVLGAVVLGCVVLFKWLPTAFVPQEDQG
                     YVFVGYFLPDSASLGRTHAVGDQARTIVAKHPAVANVAQVDGYSLIDSQLKTNFGVLF
                     VSLKNYEERKGKALSADALIADTAREFGGIQGGLVRGVNPPAIPGLGVTAGFQIWIEQ
                     KGSGNYQQLADVVGKIIAEAKKRPELAGVNTTIRTDGQQMLAEVDREKAEVLGVPVQD
                     VYSTLQTMFGSLYVSQFPKDSRLYQVVLQAEPRFRMTPEDIGRFYVRNRMGDMVPLSG
                     LVNARWVTGADLMTRFNNYPAIALNGAPAPGVSSGAALAAIREVVEQQLPSGYGYEWA
                     GEAREEVSSGSTSAIAFVFGLLFVFLILAAQYESWSLPVAVMMAVPFAILGALLAIAL
                     RGIPNDIYFQIGLLTLVGLAAKNAILIIEFAVELNRKEGMSFFDAAAEAAKLRLRPII
                     MTSFAFVLGTVPLAIATGASANSRHSIGTGVIGGTLAATLIAIFFIPMFFWLLETMSA
                     RVFGGEARPQSALRAHATGGTAPAGGGDAPAAAGSAGEGG"
BASE COUNT         1065 a         2345 c         2248 g         1056 t
ORIGIN      
        1 ccaggatctt cacgtgcgtg ccgccgacgt cgacgaccag gatcttcatg tcaccttcct
       61 cctgcggcgg tctgcgatga gcgccccggc gagcgccgtc caaccggtct ggtgcgaggc
      121 accgagcccc gcgccggtgt ccccgtggaa atattcgtgg aacagcatcg gatcgcgcca
      181 cgcgggatcc cgacggaacg acgccggtgc gtcgtcggac ggccgacgcc cgtcggcacc
      241 gcgcacgaac agcgacagca accggcgctc cagctcgtcg gcgacgtcgc agagtcggac
      301 atggactccg gagccggtgg gcaactcgac ggtaaagtcc gctccgaagc actcgtgcag
      361 gtggcgcaac gattcgagcg cgaggtagtt gaacgggaac cacaccggcc cccgccagtt
      421 cgaattgccg ccgaacagtc cgctggtgga ttccgcgggc tcgtagtcga gtcgcgcctc
      481 gccgcccggc agcgggacca cgagcgggtg gtcacggtgg taccgcgaca gcgacctcag
      541 cccgaaaggc gacaggaatt cctgctcgtc gagcatgcgc gccagcacac ggacaaggcg
      601 atcgtggtcc acgagggtca ggagcgcggg gcggcggtcg tccgggtcga agtgcaggaa
      661 ctcgccgacc cagggattgt ggtcgatgta ccagcgggca cgcgcccgga aatccgacag
      721 ctgctcccac agccgcggat cgatgcgcac cgcggcgaac aagggcagca gcccgaccat
      781 ggaacgcgcg cgcagcggga tcgtcgtgcc gtccggcttg cgcaaccggt cgtagaagaa
      841 cccgtcgtcc tcgctccaca gttcgcgcgt cgcgcgcgcg atcgagcaga agtgctcgaa
      901 gaacttgagc gccacgtcct cgtacgcgcg gtcgtggttc gcgagccgca gcgcgatctc
      961 gagcatgttg aggcagtact tggccatcca ggccgtgccg tcggattgct cgaggatcca
     1021 gccctcgggc agcggcgtgg agcggtcgaa ggcgccgatg ttgtccaggc cgaggaagcc
     1081 gccctcgaag atgttgtcgc cgagcacgtc cttgcggttg acccaccacg tgaagttgat
     1141 cagcagcttc tggaacgcgc gggcgaggaa ctcgaggtcc cggctgccgt cgatcgcgaa
     1201 cacgcgcagc gccgcccacg cctgcaccgg cgggttgacg tcgccgaagt tccattcgta
     1261 cgcgggcagc tggccgttcg gatgcatgta ccactcgcgg ccgagcagca gcagctggtg
     1321 cttggcggcc tcgggatccg tgtgcgcgag tacgatgcaa tggaacgcga ggtcccaggc
     1381 tgcgaaccac gggtactccc acttgtccgg catcgcgagg atgtcgtggt tgctcaagtg
     1441 gcgccagtcg gcgttgcgcc cggtgagccg cgacgctggc ggcggcacgg cgtcgccgtc
     1501 gagccacttc gcgacgtcga agtggtagaa ctgctggccc caggtcatcc cggcgaatgc
     1561 ctggcgcatg atgcgcgcct cctcgtcggt cgccgcggcg ggacgcagct cggcgtagaa
     1621 ctcgtccgcc tcgcgctcgc gcgtcgccag cacctgctcg aattcggtgc cgaggtcagc
     1681 ttccgcgatg tgctcctgcc gcgcgagtcg cagtcgcaac tcgacggact gaccggccgg
     1741 gacggcgagc cgataccagc aggacatctt cgacccgcgg cgagcgggat tgaccgttgc
     1801 agccccgtgg atcacgtggt cgttgatccc gtccttggga tacggggtcg atcccggcac
     1861 gccgaacagg cgtggcgcat tggtctcgtt ctcgcagaac agcagtgtcg gccgggcgcc
     1921 gcccgatggc gcgccggccg tgagcgacca gttcccgatg cgcgaatctt cgaccacggc
     1981 gatggcggca ttcccgacgt ccgggcctgc cgcgtggatc gaggggcggg gctcatcgtc
     2041 tccccacgac caccggttgc ggaaccacaa ggtcggcaac acgtgcagtt ccgccgcgtc
     2101 gggcccggca ttgtggatcg tcaggcgcat gcacacgtcg cgcggggacg ccttcgcgta
     2161 gtccgcggtg atggcccagt accggccgtc gtcgaagatg ccggtgtcgg caagttcgaa
     2221 ttcgcggtcg ccccggttgc gccgcgcatt ctcctcacga agtcgcgcgt acgggaactc
     2281 ggcctgaggg tagtggtagc gccagcgcag ccaggacgcc gtcggcgtcg catcctcgta
     2341 ccaccagcac tccttcgcgt cctcgccgtg gttgccctcg tgcccggtga gcccgaagat
     2401 ccgctccttc aggatcgggt cacggccgtt ccagaatacg agcgcgaagc acaggttctg
     2461 gccggcatcg cagagtccgg cgaggccgtc ctcgctccaa cggtaggccc gcgaccgcgc
     2521 gtggtcgtgc ggaaagtagc gccaggcatc accgtccgcg ctgtagtcct cgcgcaccgt
     2581 gccccacgcg cgctcgctga gccacggacc gaacgccttc cactcgccgg cttgccgcga
     2641 agtgctcgcg gcaagccggg cgcgctcggc tcccggtccg gctttcttcg cctgcatggg
     2701 ggtggcgacc tgcggcctgt gccaggccgc ctattcccgt gcgcctccgg caaagcatcc
     2761 gctgccgtcc tgcgggcaga ccaccacgat ggtctgtctc tggccgcagc cttccactcc
     2821 gacggtgtat tcggcgcgat acacgccgaa gcggatcgag accggggcga ccatctcgcg
     2881 cgacagcacc tgtcccgtgg cgctcgggca gttcatgtcg aacgacacgc gctgcaccgc
     2941 ggtctggacg gcctgctgct gctgcgcgtc gagctgctgg tcggtcgact cgcagcccgc
     3001 aagcccggcc aggcatgccg ctgcgatcac gattcgatgt ctcatgtcgt cactcctcgt
     3061 tgttggccat ggtccgtcgt gtcaggggct cgagccgctc acggcgactt cgcggctccg
     3121 ccggcggctg cggacgactc ggtcatgtcc gcggccttgg cgacccagcc accgcccatc
     3181 gccttgtaga tccctgcgag cgacgcacaa agatccgcgc gggctgctgc ccaggcgagt
     3241 tcctgcggga agagctgctc ctcggcctgc agcaccgtcg cgtacggggc gcgaccgacg
     3301 tcgaactgca gttgcgccat gtgctgatac ccgcgcagtg cgtcgaccag cttctcctgc
     3361 gcggcgacct gctccgcgag tgtcgtccgc gacaccagcg cattatccac gtccgcaaac
     3421 gcattcttga tggcgagctg atacgaggcg agcgccgcct gttcggccgc cttggcctgc
     3481 ttcacctgcc cgtagatcgc gccacccgag aagatcggac ccgcgacctg gcccgcgtag
     3541 ctccaggtgc ccgccgggcc cttgaacagg tcggagagtt cggtgcttgc gctgccgagc
     3601 gccccggtca acgagatcgt cgggaagaac tgcgcctccg ccgcgccgat ctgcgcattg
     3661 gcggcgacga gcagctgctc ggcctgcatc acgtccggcc ggcgctgcag caggtccgaa
     3721 ggcacgcccg cgggcacctg cggcagcgtg atgtcgaaga tcgacttgcc gcgctcgatc
     3781 ggcccagggt tgcgcccgag cgctatcgag agcccgttct cgagcaccgc gatgtcgcgg
     3841 cggatgcgcg ggatctgcgc ggtcgccgtc tggtagcgcg cacgcgcctg ctccacgttc
     3901 atctgcgaga cacggccgta cttgaacttc agttccatca cccgcagcga ctcggcataa
     3961 gtcgcctgcg tgcgctcggc catcgtgagc tgctcgtcca gcccccggag ctgcaggtag
     4021 ccggtcgcga cggtcgcgac cagcgacagg atcacgccgc gtcgcgcctc ctgggtggcg
     4081 agcaggtccg cctgagccgc ctcggtcagg cggcgaatgc gcccccacag gtcgatctcc
     4141 cacgtcgcgc ccgccagcac ctggtaggcg tcggtgggat tctcgatccc ggacgcgagg
     4201 tccgtcgtcc cgtgcgtcga gaatttgtcg cgagcggcta cgccgctgta accgacctgc
     4261 gggaacaacg gcgaccgggt ggtcgtcagt acgccggccg cctgctcgac gttggcggtg
     4321 gcgatcctga cgttccagtt gttggccagc gcctcggcga tcagcgcgtc gagcaccggg
     4381 tcgccgaact gcttccacca ctccgcgtcg gcgtcagcgg tggcctgctg cggttcgaat
     4441 cggaacgcgt ccggggactt gacttcgggt cggtggtagt cggggccgac catgcacgcc
     4501 ggcagcgcga gggacaccag gatgatcgcg gttcgggcgc gcatgtcagc ccccctcccc
     4561 ggccgatccc gcggccgccg gcgcatcccc tccgccggcc ggcgcggtgc cgccggtggc
     4621 atgcgcacgc aacgcggatt gcggccgcgc ctcgccgccg aacaccctgg cactcatggt
     4681 ctcgagcagc cagaagaaca tcgggatgaa gaagatcgcg atcagcgtcg cggcgagcgt
     4741 gccgccgatc acgccggtgc cgatcgaatg ccggctgttg gccgacgcgc cggtcgcgat
     4801 ggcgagcggc acggtgccga gcacgaacgc gaaggacgtc atgatgatcg gccgcagtcg
     4861 cagcttcgcc gcctcggctg ccgcgtcgaa gaacgacatg ccttccttgc ggttcaactc
     4921 gaccgcgaac tcgatgatca ggatggcgtt cttcgcggcg aggcccacca gcgtgagcaa
     4981 gccgatctgg aagtagatgt cgttcgggat gccgcgcagc gcgatggcga gcagtgcccc
     5041 caggattgcg aacggcacgg ccatcatgac tgcgaccggc agcgaccagc tctcgtactg
     5101 ggccgcgagg atcaggaaca cgaacagcag gccgaacacg aatgcgatcg ccgacgtcga
     5161 gcccgacgag acctcttcgc gcgcctcgcc ggcccactcg tagccgtagc cggagggcag
     5221 ctgttgctcc acgacctcgc ggattgccgc gagcgccgcg ccggagctga cgccgggcgc
     5281 cggcgcgccg ttgagcgcga tcgccgggta attgttgaag cgggtcatga ggtcggcacc
     5341 cgtcacccag cgcgcattga cgagccccga cagcggcacc atgtcgccca tgcggttcct
     5401 gacgtagaac cggccgatgt cctcgggcgt catgcggaac ctgggctccg cctgcagcac
     5461 cacctggtag aggcgcgagt ccttcggaaa ctggctgacg tacagcgagc cgaacatcgt
     5521 ctgcagcgtg ctgtagacgt cctgcaccgg cacgcccagc acctcggcct tctcgcggtc
     5581 gacctccgcg agcatctgct ggccgtcggt gcggatcgtg gtgttcacgc ccgcgagctc
     5641 cgggcgcttc ttcgcctcgg cgatgatctt gccgaccacg tcggccagct gctggtagtt
     5701 gcccgagccc ttctgctcga tccagatctg gaagccggcc gtgacaccga ggcccgggat
     5761 ggcagggggg ttgacgcccc gcaccagtcc gccctggatt ccgccgaatt ccctggccgt
     5821 gtcggcgatc agcgcgtcgg cggacagggc cttgcccttg cgctcctcgt agttcttcag
     5881 cgacacgaac agcacgccga agttggtctt caactgcgag tcgatcaggc tgtagccgtc
     5941 cacctgggcg acattggcca ccgccgggtg cttggccacg atcgtgcgcg cctggtcgcc
     6001 gaccgcgtgc gtgcggccga ggctcgccga gtccggcagg aagtagccga cgaacacgta
     6061 gccctggtct tcctgcggca cgaacgccgt gggcagccac ttgaacagca ccacgcagcc
     6121 cagcaccacg gcgcccagca cgccgaggcc acgcacccag ttgtgcgtca cccacttgac
     6181 gccgccgacg taggagtccg tgagactcgt cagcgtgtcg tcgaaccact tgaagaatcc
     6241 cttcttttcc tcgtgccgcg cctggatcag gatcgcggcg agcgccggcg acaacgtgag
     6301 cgccaccagt cccgagaaca ccacggagat cgcgatggtg acggcgaact gcttgtagag
     6361 cgcgccggtg atgccgccga ggaacgccac cgggatgaac accgcgttca gcaccagcac
     6421 cacggcgatc acggggcccg acacctcgtc catggcgcgc ttggccgctt ccttcgcgga
     6481 gaggttgaac tgcaccatgt tgcgctcggt gttctcgacc acgacgatcg cgtcgtccac
     6541 gacgaggccg atcgcgagaa tcatgccgaa cagggtcagc atgttgatcg agaagccgaa
     6601 cagcagcatg ccgaccagcg cgccgaggat cgagaccggc accgccagcg tcgggatgat
     6661 cgtgagccgc atgctgtgca ggaacacgaa cacgaccagc accacgagca ccac
//
LOCUS       DAOMFD010000107         6601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4233_length_6601_cov_35.633975, whole genome shotgun sequence.
ACCESSION   DAOMFD010000107 DAOMFD010000000
VERSION     DAOMFD010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6601
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4233_length_6601_cov_35.633975"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1256
                     /locus_tag="PL152_07215"
     CDS             <1..1256
                     /locus_tag="PL152_07215"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008746 - NAD(P)+ transhydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HQR49109.1"
                     /translation="VLAIDSLPRQLSRAQKMDALTSMASISGYRAVIEAANAFGRFFN
                     GQITAAGKIPPAKVFIAGAGVAGLAAIGTAANLGAIVRANDTRAEVADQVVSLGGEFV
                     KVDYEEEGSGGGGYAKVMSEGFQQAQRAMYAKQARDSDIIITTALIPGKPAPKLITAD
                     MVKSMKPGSVIVDMAAEQGGNCELTVPGEAVVRHGVTIIGYTDLASRLAKQSSTLYST
                     NLLRLTEELCKTKDGVIDVNMDDDAIRGLTVIKDGAITWPPPPPKLPAAPPQAKPAAA
                     APTPAKKSGHGHGGAGEPASVKSVAVMFGVGALLFWLVGAFAPATFLAHFTVFVLACF
                     VGYMVIWNVTPSLHTPLMSVTNAISSIIAVGALIQVAPPLVEGTEGRPDRLILGLAVL
                     ALTLTAINMFGGFAVTRRMLAMFRK"
     gene            1270..2682
                     /gene="pntB"
                     /locus_tag="PL152_07220"
     CDS             1270..2682
                     /gene="pntB"
                     /locus_tag="PL152_07220"
                     /inference="COORDINATES: protein motif:HMM:NF006974.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008750 - NAD(P)+ transhydrogenase
                     (AB-specific) activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HQR49110.1"
                     /translation="MTANIATVAYIAATILFILSLGGLSNPETSRRGNVYGIIGMTIA
                     VLATAFGPRVTMAGIPWIVGALLVGGSIGLYLARKVQMTQMPELVAVMHSLVGLAACL
                     VGYASYVDTSTVFPTAAEKSIHEIEIYLGVLIGAVTFSGSVIAYGKLSAKIGGKPLLL
                     PARHWLNLAGLLVVIWFGVKFLQAPDIETGMTPLIVMTAIALLFGVHMVMAIGGADMP
                     VVVSMLNSYSGWAAAATGFMLSNDLLIVTGALVGSSGAILSYIMCRAMNRNFISVIAG
                     GFGTGEGAAPAAAGAQPAGEVTAISAVETAELLRDAKNVIIVPGYGMAVAQAQHTVYE
                     ITKLLREKGVNVRFGIHPVAGRMPGHMNVLLAEAKVPYDIVMEMDEINDDFPDTDVSM
                     VIGANDIVNPAAEDDPTSPIAGMPVLQVWKAKTSIVMKRSMASGYAGVDNPLFYKENN
                     RMLFGDAKKMLEEVLAALRG"
     gene            2854..3810
                     /locus_tag="PL152_07225"
     CDS             2854..3810
                     /locus_tag="PL152_07225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49111.1"
                     /translation="MKRTVTLILLSVCSAAFAKDAPVFDPAADPAALAASFVEVSNIK
                     ALAGTKKVAVSQFRVEFAVENSAKATSSSSAGWTSTSSDVKLVGVPTETLQQVADAME
                     DRFVEELGKAGLEVVPYDVLKEDAAYKSLAPVLRTSNEPLGTQTGESIFVGGHGTPYY
                     LTNDDKHLGLGTMLGAFSTTQPQNIEPSIAKQLDAAVLRVTVMVAFADQKTSGGLFHS
                     GSSVKTELGLTIVPEITGMLVITPASGKTRATLDESVVMGSEIFDLRTTKKEGFAILT
                     GFRGTKNYDAVTTPEAYSDLVTKYGQALQSAMVAAIRPGLGP"
     gene            3880..>6601
                     /gene="ppc"
                     /locus_tag="PL152_07230"
     CDS             3880..>6601
                     /gene="ppc"
                     /locus_tag="PL152_07230"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF000584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HQR49112.1"
                     /translation="MDPASDSDRPLREDIRLLGRLLGDTVREQVGDGAFELVEHIRQS
                     SIAFRRDDDVEARRELERILDGLSREQTMVVARAFSYFSHLANLAEDVDTIRRSRRSA
                     IDGDPPPEGSMVRALDRVSTAGFGPDALAAFFADALVVPVLTAHPTEVQRKSVRDLEM
                     NIERLLRARDRTRVTPAEADANDEEVRRAVLALWQTRILRLEKLGVIDEVANGLSFFE
                     QTFLPEVPRLYGALEDELQRREPARAWDELPSFLRIGSWIGGDRDGNPFVTAEVLRHT
                     LRMQSSRVFEFYLDELHALGAELSLTTLATGVTPELLALAERSPDTSPHRRDEPYRRA
                     LTGLYARVAATTRALEQHEPLRHAVGDAPPYARAGELAADLDVIHRSLVAHGSSLIAR
                     GRLRRLRRAVGVFGFHLAAVDLRQNSDVHARVVAELLTAARPEVDYSRLDEDARIRLL
                     VEELASPRLLASPYLDYSAETRSELEILRAAADMHRRYGPECIPNYVISKCDGVSDVL
                     EVVVLLREAGLFRAADARLQCNVVPLFETITDLQGAARTMDRLMAIPVYARMLAGRGS
                     AQEVMLGYSDSNKDGGYLTSRWELFKAQTGLVGTFRRHGVRLRLFHGRGGSVGRGGGP
                     SYEAIVAQPQGTVQGQIRITEQGEVIGVKYSNPEVGRHNLEILAAATLEATLLGGRGE
                     APPPAYHEALESLSTLALAAYRGLVYETPGFEDYFWESTVISEIAELNIGSRPASRTQ
                     SRSIETLRAIPWVFSWSQCRLMLPGWYGFGTAVTEWLANRADGLALLNEMNRTWPFFA
                     SVLSNMEMVLAKADLAIASRYAALVNDATLREAIFRRIRAEFERTTEVLLRILGANSL
                     LAGNQSLAESIRYRFPYLDPLNHIQIELLRRYRSGDREERSRRGIHLTING"
BASE COUNT         1074 a         2292 c         2214 g         1021 t
ORIGIN      
        1 ccgtgctcgc gatcgactcc ctgccgcgcc agctctcccg cgcccagaag atggacgcgc
       61 tcacctccat ggccagcatc agcggctacc gcgccgtgat cgaggcggcc aacgccttcg
      121 gccgcttctt caacggccag atcaccgccg ccggcaagat cccgccggcc aaggtcttca
      181 tcgccggcgc cggcgtcgcg ggcctggccg ccatcggcac ggccgccaac ctcggtgcca
      241 tcgtgcgcgc gaacgacacg cgcgccgaag tcgccgacca ggtcgtgtcg ctgggcggcg
      301 aattcgtcaa ggtggattac gaggaggaag gctccggcgg cggcggctac gccaaggtga
      361 tgagcgaggg cttccagcag gcccagcgcg cgatgtacgc gaagcaggcc agggactccg
      421 acatcatcat cacgactgcg ctgattcccg gcaagccggc gccgaagctc atcaccgccg
      481 acatggtgaa gagcatgaag ccgggcagcg tcatcgtgga catggcggcc gagcagggcg
      541 gcaactgcga gctcacggtg ccgggcgagg ccgtcgtgcg gcacggcgtc accatcatcg
      601 gctacacgga tctcgccagc cgcctcgcca agcagtcgtc gacgctctat tcgacgaacc
      661 tgctgcgcct caccgaggaa ctctgcaaga ccaaggacgg cgtcatcgac gtcaacatgg
      721 acgacgatgc catccgcggg ctgaccgtga tcaaagacgg cgcgatcacc tggcctccac
      781 cgccgcccaa gctgccggct gcaccgccgc aggcgaagcc agcggcggcg gccccgaccc
      841 cggcgaagaa gtcaggccat gggcacggcg gcgccggcga gccggcgtcc gtgaagagcg
      901 ttgccgtgat gttcggcgtc ggcgcgctgc tcttctggct ggtcggtgcc tttgcaccgg
      961 cgacgttcct cgcgcacttc accgtcttcg tgctggcgtg cttcgtcggc tacatggtga
     1021 tctggaacgt cacgccctcg ctgcacacgc cgctcatgag cgtgaccaac gcgatctcga
     1081 gcatcatcgc cgtcggcgcg ctgatccagg tggcgccgcc actcgtcgag ggaaccgagg
     1141 gtcgccccga caggctgatc ctcggcctcg cggtgctcgc gctcacgctc accgccatca
     1201 acatgttcgg cggcttcgcc gtcacgcgtc gcatgctcgc gatgttccgg aagtaagggg
     1261 agcgcaggca tgaccgcaaa catcgcaacc gtggcctaca tcgccgcgac catcctgttc
     1321 atcctgagcc tcggcgggct gtcgaatccc gagacgtccc ggcgcggcaa cgtgtacggc
     1381 atcatcggca tgaccatcgc cgtgctcgcg acggcgttcg ggccgcgcgt caccatggcc
     1441 ggcatcccgt ggatcgtcgg cgcgctgctg gtcggcggct cgatcggcct gtacctggcg
     1501 cgcaaggtgc agatgacgca gatgccggag ctggtcgccg tgatgcacag cctcgtcggc
     1561 ctcgcggcgt gcctcgtcgg ctacgcgagc tacgtcgaca cctccacggt gttcccgacg
     1621 gccgccgaga agtccatcca cgagatcgag atctacctgg gcgtgctgat cggcgccgtg
     1681 acgttctcag gctccgtcat cgcgtacggc aagctctccg cgaagatcgg cggcaagccc
     1741 ttgctgctgc cggcgcggca ctggctcaac ctcgccgggc tgctggtcgt gatctggttc
     1801 ggcgtgaagt tcctgcaggc gccggacatc gagaccggca tgacgcccct catcgtgatg
     1861 accgcgatcg cgctcctgtt cggcgtgcac atggtgatgg cgatcggcgg cgcggacatg
     1921 ccggtggtcg tgtcgatgct gaacagctac tcggggtggg ctgcggcggc caccgggttc
     1981 atgctgtcga acgacctgct gatcgtgacc ggagcgctgg tcggatcgtc gggcgccatc
     2041 ctgtcgtaca tcatgtgccg cgcgatgaac cgcaatttca tcagcgtcat cgcgggcggc
     2101 ttcggcacgg gcgagggcgc ggcgccggcg gcagcgggtg cgcagccggc cggcgaggtg
     2161 acggcgatca gcgccgtgga gaccgccgag ctgctgcgcg atgcgaagaa cgtgatcatc
     2221 gtgccgggct acggcatggc ggtcgcgcag gcgcagcaca ccgtgtacga gatcacgaag
     2281 ctgctgcggg agaaaggcgt gaacgtgcgc ttcggcatcc acccggtcgc gggccgcatg
     2341 ccaggccaca tgaacgtgct gctcgccgag gcgaaggtgc cgtacgacat cgtgatggag
     2401 atggacgaga tcaacgacga tttccccgac accgacgtct cgatggtgat cggcgccaac
     2461 gacatcgtga atccggcggc ggaggatgac ccgaccagcc cgatcgccgg catgccggtg
     2521 ctgcaggtgt ggaaggcgaa gacctcgatc gtgatgaagc gctcgatggc ctcgggctac
     2581 gccggcgtcg acaatccgct gttctacaag gagaacaacc ggatgctgtt cggcgacgcg
     2641 aagaagatgc tcgaagaggt cctcgctgcg ctcaggggct gatcgcgccg cacgcctcgc
     2701 tcccgcccac cggaacgccg aggggcgtcg ggattcccgc ccccccctgt tgtcaacccg
     2761 cggcgcgcgc cggtaggctt ggccgcgaca gggggacgct cgctcgcgac gagccacaat
     2821 gaggcgcccc cgccgggcaa agggggccca cacatgaaac gcaccgtcac gctgatcctg
     2881 ctatcggtct gctccgccgc gttcgccaag gatgcaccgg tcttcgaccc ggccgccgat
     2941 cccgccgcgc tggcggcgag cttcgtcgag gtgagcaaca tcaaggcgct cgccggcacg
     3001 aagaaggtgg ccgtctcgca gttccgcgtc gagttcgcgg tcgagaattc cgccaaggcg
     3061 acgtcgtcca gcagcgccgg gtggacctcc accagttcgg acgtgaagct cgtcggcgtt
     3121 cccaccgaga cactgcaaca ggtcgccgac gccatggagg accgcttcgt cgaggaactc
     3181 ggcaaggcgg gactggaggt cgtcccctac gacgtgctca aggaggacgc ggcctacaag
     3241 tcgctcgcgc ccgtgctgcg cacgtcgaac gagccgctcg gcacgcagac cggcgagtcc
     3301 atcttcgtcg gcgggcacgg gacgccctac tacctgacca acgacgacaa gcatctcggc
     3361 ctcggcacga tgctcggtgc cttcagcacg acgcagccgc agaacatcga gccctcgatc
     3421 gcgaagcagc tcgacgccgc cgtgctgcgc gtgacggtga tggtcgcctt cgcggaccag
     3481 aaaaccagcg gcggactgtt ccactccggt tcgtcggtca agaccgagct cgggctgacg
     3541 atcgtgcccg agatcaccgg gatgctcgtc atcacgccgg cctcgggcaa gacgcgcgcg
     3601 acgctcgacg agagcgtggt gatgggcagc gagatcttcg acctgcgcac caccaagaag
     3661 gaaggcttcg cgatcctgac cggctttcgc ggcaccaaga actacgacgc cgtgaccacg
     3721 ccggaggcct actcggatct cgtgacgaag tacggccagg cgctgcagtc ggcgatggtg
     3781 gcggcaatca ggccgggcct cggaccgtag gcaaccccgg gcgtcccggc cctccccggg
     3841 gccgaagcgc cggccggcac ggctgggcta agctagggca tggaccccgc ctcggactca
     3901 gatcgccccc tgcgcgagga catccgcctg ctcgggcgcc tgctcggcga cacggttcgc
     3961 gagcaagtag gtgacggcgc cttcgaactt gtcgagcaca tccgccagag ctcgatcgcc
     4021 ttccgccgtg acgacgacgt cgaggcgcga cgggaactgg aacgcatcct agacggcctg
     4081 agccgcgaac agacgatggt ggtcgcgcgc gcattcagct acttctcgca cctcgccaat
     4141 ctcgcggagg acgtcgacac gatccgccgc agccgacgtt cggcgatcga tggcgacccg
     4201 ccgccggaag gcagcatggt gcgggcactc gaccgcgtct cgacggccgg gttcgggccg
     4261 gatgcgctcg cggcgttctt cgccgatgcg ctggtcgtcc cggtgctcac tgcgcatccg
     4321 accgaggttc agcgcaagag cgtgcgcgac ctggaaatga acatcgagcg cttgctccgc
     4381 gcgcgcgatc gcacacgagt gacccccgcg gaggccgacg ccaacgacga ggaggtccgg
     4441 cgcgcggtcc tcgctttgtg gcagacacgg atcctgcggc tggagaagct gggcgtgatc
     4501 gacgaggtcg ccaacggcct gtcgttcttc gagcagacct tcctgcccga ggtccctcgc
     4561 ctctacggcg ccctcgagga cgaactgcag cgccgggagc cggcccgggc gtgggacgaa
     4621 ctgccgtcgt tcctgcgcat cggctcgtgg atcggggggg accgcgacgg caatccgttc
     4681 gtcactgcgg aggtgctgcg acacaccttg cgcatgcagt cctcgcgggt cttcgagttc
     4741 tacctggatg agctgcacgc cctgggcgcc gaactttcac tgacgacact ggcgaccggg
     4801 gtcacgccgg agctcctcgc gctggccgag cggtccccgg acacgagccc tcatcgccgc
     4861 gatgagccct accgtcgggc gctgaccggc ctgtacgcgc gagtcgccgc gacgacccgc
     4921 gcgctcgagc agcacgagcc gctgcgccat gcggtgggcg acgcgcctcc ctatgcccgt
     4981 gccggcgaac tggcggcgga tctcgacgtc atccaccgct cgctcgtggc acacggctcg
     5041 tcgttgattg cccgcggcag gctgcgccgc ctgcggcgcg cggtcggcgt attcgggttt
     5101 catctcgccg cggttgacct gcgacagaat tccgacgttc acgcccgcgt cgtcgctgag
     5161 ttgcttacgg cagcgaggcc ggaggtggac tattcacgcc tcgacgaaga cgcccgcatc
     5221 cgcctgctgg tcgaggagct ggcctcgccc cgcctgcttg cttcgccgta cctggactat
     5281 tccgcagaga cgcgcagcga gctcgagatc ctgcgggccg cggccgacat gcatcggcgc
     5341 tacggacccg aatgcatccc gaactacgtc atctcgaagt gcgacggcgt gtccgacgtg
     5401 ctcgaggtcg tggtcctgtt gcgcgaggcc gggctgttcc gcgcggccga cgcgcgcctg
     5461 caatgcaacg ttgtcccgct gttcgagacc atcacggacc tgcagggggc ggcccgcacg
     5521 atggaccggc tgatggccat cccggtctac gcccgcatgc tcgcgggccg cggctccgcg
     5581 caggaggtca tgctcggcta ctccgacagc aacaaggacg gtggttacct cacgtcacgc
     5641 tgggaattgt tcaaggcgca gaccgggctg gtcgggacgt tccggcggca cggagtacgg
     5701 ttgcgcctgt tccacggccg cggcggctcg gtggggcgcg gcggcgggcc gagctacgag
     5761 gcgatcgtcg cgcagccgca aggaacggtg cagggacaga ttcgcatcac cgagcagggc
     5821 gaagtcatcg gcgtgaagta ctcgaacccg gaggtcgggc ggcacaacct cgagatcctc
     5881 gccgcggcga cgctcgaggc caccctgctc ggcggccgtg gcgaggctcc gccgcccgcg
     5941 taccacgaag ccctcgagtc gctctcgacg ctggcgctcg cggcttaccg tggcctcgtc
     6001 tacgagacgc cgggattcga ggactacttc tgggaatcga ccgtcatcag cgagatcgcg
     6061 gaactcaaca tcggcagccg ccccgcctcg cgcacccagt cgcgctcgat cgaaacgctg
     6121 cgtgccatcc cctgggtgtt ctcgtggtcg cagtgccggt tgatgctccc gggctggtac
     6181 gggttcggca ctgcagtcac cgagtggctc gcgaatcgcg ccgacgggct cgcactgctc
     6241 aacgagatga accggacctg gccgttcttc gcttccgtcc tgtccaacat ggaaatggtc
     6301 ctcgccaagg cggacctcgc catcgcctcc cgctatgcgg cgctggtcaa cgacgcgacg
     6361 ctgcgcgagg ccatcttccg caggatccgc gccgaattcg agcggaccac cgaggtactg
     6421 ctgcgaatcc tgggcgcgaa cagtctactg gcaggaaacc agagtctggc cgaatcgatc
     6481 cgctaccggt ttccgtacct cgaccccctc aatcacatcc agatcgagct gctgcgccgc
     6541 taccggtccg gcgaccgcga ggagcgcagc cggcgcggga tccatctgac gatcaacggc
     6601 a
//
LOCUS       DAOMFD010000108         6595 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4238_length_6595_cov_36.109174, whole genome shotgun sequence.
ACCESSION   DAOMFD010000108 DAOMFD010000000
VERSION     DAOMFD010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6595)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6595
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4238_length_6595_cov_36.109174"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1914
                     /locus_tag="PL152_07235"
     CDS             <1..1914
                     /locus_tag="PL152_07235"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HQR49113.1"
                     /translation="AGDASGPAASAPSTAPPFEWKNVERVVAFADVHGAYDELVALLQ
                     SAGVVDARLNWSAGKTHLVSLGDLLDRGDDCRKVMDLLMRLQREAAAAGGQVHVVVGN
                     HEAMNVLGDLRYVSRGDYAQYVEDEDAASRDEQRRQFLARNPGKSDAYFDRVFPPGYF
                     GQRKLLGPGGKYGRWILAQPAIIVVNDTVFMHGGPSLALGGRSIAQLDRDYDAALETY
                     LASEAALRDAGLLEPEDPYGRRPDLAQARLGAMPADERRGALAPLVAQFARADENPLL
                     GPDGPNWYRGAALCNECAEADVLRPFLAAAGARRVVVGHTVARDGTAVARFDGALIKL
                     DAGMNKAIYHGRPAALILDSSGIRVAYANPTVPPADVPRETLYLSSPTMDENTVAAVL
                     AAGSIEPTETCGPGIVRVRVTQEGKSVDAVFEAAPAKTVGHELAAWRLDQLLGLGLVP
                     ATVARTYGGAAGVLQGRPASWATEQDRQNLANGARAGLACQVISNDPRVQPIRRPLAT
                     AAVPARLPSGGYCDIKAQYQLAYAFDALIGNQARTFDRYLYDLDPSMQARGLSLILSG
                     HGSAFGSQVQLPSAIQGPLAGTGPEMQSRLRKLDAAAVKAAIGQWVSDAEIKALMARR
                     DRILEITRAPAAN"
     gene            complement(1964..3619)
                     /locus_tag="PL152_07240"
     CDS             complement(1964..3619)
                     /locus_tag="PL152_07240"
                     /inference="COORDINATES: protein motif:HMM:NF015194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005471 - ATP:ADP antiporter activity
                     [Evidence IEA];
                     GO_process: GO:0006862 - nucleotide transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Npt1/Npt2 family nucleotide transporter"
                     /protein_id="HQR49114.1"
                     /translation="MLIDQSGLSRFERFLSLFTRVRPGEGPGVLMFAAHAFLMLFSYQ
                     VMKALREALVLAKYSAEMRSYGVAITALVLMLVVPLYGVVRRRFDGARLLRGVTVFFA
                     VNLWIFAAAVWAGVSVAFVFFVWASIYGIMVVSQLWAFAADCMNVKSGQRLFPVIMVG
                     ANLGALAGAKTAQLAVAALTPVGLIIASSLLLLATLWLIGPERAAIPEGSRAIAPEHG
                     ASTPRLLGGIGLVLRDRYLLLVALLVVLLNWINTTGEFILADFVQHDAAARATASGGA
                     LDPSVLIAEFYGSYQFWVTVVGLAIQLFLVARIYRAVGVKGALVIHPSVVAIGYGLLT
                     FSTMLVGFIPVFTLIRLVKITENATDYSLMNTTRQALFLPVDRDAKYEGKTAIDTFYW
                     RIGDVIQAGVLYVGLNLLGWHAPAFAAVNLVLALAWIGLAVAIGREFGRKAQECVTNV
                     APEARQPIADLLYVPGSPVEHFVLEDAFVDADPGDVLRLTARLADGKALPGWLRFDAP
                     RRRFHGTAPDGHVEEFMVVVVACDVDGLEATSSFVVRRAMLRD"
     gene            complement(3625..4797)
                     /locus_tag="PL152_07245"
     CDS             complement(3625..4797)
                     /locus_tag="PL152_07245"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR49115.1"
                     /translation="MKKRTRVTHLPDVPVPPDNRALVAPIYQSVKFTFDDVAETRKAW
                     SGQRDGFYYSRDGNPTLRQLELVLAELQQRDACLLTASGIASITTTLLALLKSGDHVV
                     YFAESYSPTRSFVRRVLGRYGVSATMLSVTDHDALERTLAATPTRLIVFESPTNPVLK
                     VADLERVTAVARRHGALTLLDNTLAGLHNHGQYDVDLFAHSLTKYASGHGDVMGGAVI
                     GRRELIDAMREDFVQLGPTLDPHAAYLMLRGLKTYFVRWEVQCRNAAVVAEFLASHEA
                     VARVRYPGLASDPGHDLATRQMDGFGTIVTFDIAAGLEAGTRFAEALELFAIASSLGS
                     TESLVIPPALQQPRGFTPQQLAWSDIGPGTVRLSVGVEDPDDLVEDLRRALARSTG"
     gene            complement(4794..5570)
                     /locus_tag="PL152_07250"
     CDS             complement(4794..5570)
                     /locus_tag="PL152_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HQR49116.1"
                     /translation="MNQHLRVTLVQADLAWRDPEANRHRLAAHFRGLAGHTDLVVLPE
                     MFSTGFSMDAAALAEPMDGPTVGWMREEAAAVGCVITGSLIVRDGDRHVNRLVWARPD
                     GSLEHYDKRHLFRMAGEHEHYRPGDRRIVVNVKGWKVCPLVCYDLRFPVWSRNRGDYD
                     LLLYVANWPARRAHAWSALLRARAIENLCYVAGVNRIGRDGNGVAYAGDSVVLDFLGQ
                     PVSSEGAGDRVETAVLDLQSLRAWRESFPAHLDADGFELT"
     gene            complement(5570..>6595)
                     /locus_tag="PL152_07255"
     CDS             complement(5570..>6595)
                     /locus_tag="PL152_07255"
                     /inference="COORDINATES: protein motif:HMM:NF006569.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HQR49117.1"
                     /translation="RLVELVAQHMREGRNQYAPMAGWPALVEAIAAKVRALYGRTVSP
                     GDEVTVTSGGTEALFCAVQAVVRTGDEVILLEPAYDSYEPAVQLAGGRAVRVPLEPPT
                     FAVDWERVRAAISRRTRLVIVNSPHNPSGAVWSRADLDALAAALRGTDALVLSDEVYE
                     HMVFDGRSHESVLEHEELAARSFVVSSFGKTYHATGWKVGYCIAPAALTAEFRKVHQF
                     VQFAVATPMQAALAGFLRECPQHYLELAGFYQGKRDHFCQQLEGTALRFTPSAGTYFQ
                     LADYSRVSVLPDVEFARWLTREIGVAAIPISVFSSRPLNMSLVRFCFAKSAETIEAAG
                     ERLRRLQ"
BASE COUNT         1117 a         2309 c         2232 g          937 t
ORIGIN      
        1 gcaggcgacg cctccggtcc cgcggcgagt gcgccttcga ccgcgccgcc gttcgagtgg
       61 aagaacgtcg agcgcgtcgt ggcattcgcg gacgtgcacg gcgcttacga tgaactcgtg
      121 gcattgctcc agtctgccgg cgtcgtcgac gcccgcctga actggtcggc cggcaagacg
      181 cacctcgtga gcctcggtga tctcctcgat cgcggcgacg actgccgcaa ggtcatggac
      241 ctgctgatgc gcctgcagcg ggaggccgcg gcggccggcg gccaggtcca cgtcgtggtc
      301 ggcaaccacg aggccatgaa cgtgctcggc gacctccgct acgtgagtcg cggggactac
      361 gctcagtatg tcgaggacga ggacgccgcg tcacgcgacg agcagcgcag gcaattcctg
      421 gcgcgcaatc ccgggaagtc ggatgcgtac ttcgacaggg tcttcccgcc gggctatttc
      481 gggcagcgca aactgctcgg tcccggcggc aagtacggac gatggatcct cgcgcagccc
      541 gcgatcatcg tggtcaacga caccgtgttc atgcacggcg gaccgtcgct ggcactcggc
      601 ggccgcagca tcgcgcagct cgatcgcgac tacgacgccg cgctcgagac ctacctcgcg
      661 agcgaggcgg cgctgcgcga tgcaggactg ctcgagcccg aggacccgta cggccgtcgc
      721 ccggacctgg cccaggcaag gctcggcgcg atgccagcgg acgaacggcg gggggcgctg
      781 gcaccgctgg tcgcacagtt cgcacgggcc gacgagaacc ccctgctagg gcccgacgga
      841 cccaactggt atcgcggtgc agccctctgc aacgaatgcg ccgaagcgga cgtgctgcga
      901 ccgttcctgg ccgcagcggg cgccaggcgc gtcgtggtcg gccacaccgt ggcgcgtgac
      961 ggcaccgccg tcgcgcgctt cgacggcgcg ctgatcaagc tcgacgccgg gatgaacaag
     1021 gcgatctacc atggaaggcc ggcggcgctg atcctcgatt cgagcggcat cagggttgcc
     1081 tatgcgaatc cgacggtgcc gccggccgac gtccctcgcg agacgctgta cctgtcgtca
     1141 ccgaccatgg acgagaatac cgtcgcggcg gtgcttgcgg ctggctcgat cgagcccacc
     1201 gagacctgcg ggccgggcat cgtgcgggtc cgcgtgacgc aggaaggcaa gagcgtcgat
     1261 gcggtcttcg aggcagcacc cgcgaagacg gtcggtcacg agcttgcggc gtggcgcctc
     1321 gaccagctgc tcgggctcgg cctggtcccg gcgacggtgg cgcgcaccta tggcggcgcc
     1381 gccggtgtgc tgcagggccg accggcgagc tgggcgaccg agcaggaccg gcagaatctc
     1441 gcgaacggcg cccgagccgg gctcgcctgc caggtcatct ccaatgaccc gcgcgtccag
     1501 ccgatcaggc gaccactggc cacggcagcc gtgcccgcgc gcctgccgtc gggcggatac
     1561 tgcgacatca aggcgcaata ccagcttgcg tacgcgttcg acgcgctgat cggcaaccag
     1621 gcgcgcacgt tcgaccgcta cctgtacgac ctcgacccct ccatgcaagc ccggggactg
     1681 tcgttgatcc tgagcgggca cggatcggca ttcggcagcc aggtgcagct cccctccgcc
     1741 atccaggggc cactggccgg caccggcccc gagatgcagt ctcgcctgcg caagctcgat
     1801 gcagccgcgg tcaaggcggc gatcggccag tgggtgagcg acgcggagat aaaggcgctg
     1861 atggcccgcc gcgaccggat cctcgagatc acgcgcgcgc cggctgcgaa ctgagcgcgc
     1921 ggcgaggcct gaactgcgaa cgcagccacg gccggggcgc gcgtcaatcg cgcagcatgg
     1981 ccctgcgcac cacgaacgag ctggtggctt cgagtccgtc cacgtcgcat gcgacgacga
     2041 ccaccatgaa ttcctccaca tggccatccg gagccgtgcc atggaagcgt cgacgcggcg
     2101 cgtcgaaacg cagccagccc ggcagggcct tcccgtccgc gagtcgcgca gtcaggcgca
     2161 gcacgtcgcc cggatcggcg tcgacgaatg cgtcctcgag cacgaagtgc tcgaccgggc
     2221 tgcccggcac gtacagcagg tccgcgatgg gctgccgggc ctcgggcgcg acgttggtga
     2281 cgcactcctg cgccttgcgc ccgaattcgc ggccgatcgc gaccgccagc ccgatccacg
     2341 cgagcgccag caccaggttg accgcggcga acgccggcgc atgccagccg agcaggttga
     2401 gcccgacgta cagtacgccc gcctggatca cgtcgccgat gcgccagtag aacgtgtcga
     2461 tcgcggtctt gccctcgtac ttcgcgtcgc ggtccaccgg gaggaacagg gcctggcgcg
     2521 tcgtattcat cagcgagtag tcggtcgcgt tttccgtgat cttcaccagc cggatcagcg
     2581 tgaagaccgg gatgaaaccc acgagcatgg tcgagaacgt cagcagtccg taaccgatcg
     2641 ccacgaccga cgggtggatc acgagcgcgc ccttgacccc gacggcgcgg tagatgcgcg
     2701 ccacgaggaa cagctggatc gcgaggccaa cgaccgtgac ccagaactgg tagctgccgt
     2761 agaactccgc gatcaggacg ctggggtcca gcgccccgcc actcgccgtc gcgcgcgcgg
     2821 ctgcgtcgtg ctgcacgaaa tcggcgagga tgaattcgcc ggtggtgttg atccagttga
     2881 gcagcacgac gagcagcgcg accagcagca ggtaacggtc cctcagcacg aggccgatgc
     2941 cgccgagcag cctcggggtg gacgcgccgt gctcgggtgc gatcgcacgc gaaccctcgg
     3001 gaatcgccgc gcgctcagga ccgatgagcc acagcgtcgc cagcagcagc agcgacgacg
     3061 cgatgatcag ccccacgggc gtcagcgcgg ccaccgccag ctgagcggtc ttggcgccgg
     3121 ccagtgcacc caggttggcg ccgaccatga tcaccgggaa caggcgctgg ccgctcttga
     3181 cgttcatgca gtcggccgca aaagcccaca gctgcgaaac gaccatgatc ccgtagatgc
     3241 tcgcccagac gaagaacacg aaggcgacgg acacgccggc ccagacggcg gccgcgaaga
     3301 tccacaggtt gaccgcgaag aacaccgtca ccccgcgcag caggcgtgcg ccgtcgaacc
     3361 tgcggcgtac caccccgtag agtggcacca ccagcatgag caccagcgcc gtgatcgcga
     3421 cgccgtacga ccgcatctcc gccgagtact tcgcgagcac cagcgcctcg cgcagcgcct
     3481 tcatcacctg gtaggagaac agcatcagga acgcgtgcgc cgcgaacatc agcacgcctg
     3541 gtccctcgcc gggccgcacg cgcgtgaaca gcgacaggaa tcgctcgaag cggctgagtc
     3601 cggactgatc gatcagcatc gggtttaccc ggtcgaccgc gccagtgcgc gtcgcaggtc
     3661 ttccacgagg tcatcggggt cctcgactcc gaccgacagg cgcacggtgc ccggaccgat
     3721 gtccgaccac gccagttgct gcggggtgaa gccgcgcggc tgctgcagtg ccggtgggat
     3781 gaccagcgac tccgtcgagc ccaggctcga cgcgattgcg aacagctcga gtgcttcggc
     3841 gaaccgggtg cctgcctcga gaccggccgc gatgtcgaac gtgacgatcg tgccgaagcc
     3901 gtccatctgc cgtgtcgcaa gatcgtggcc cggatccgac gcgaggccgg gatatcgcac
     3961 tcttgcgacc gcctcatgtg aagccaggaa ttcggccacg accgcggcat tgcggcattg
     4021 gacctcccag cgaacgaaat aggtcttgag ccctcgcagc atcaggtaag ccgcgtgcgg
     4081 gtcgagtgtc ggaccgagct ggacgaagtc ttcgcgcatc gcatcgatga gttcccgccg
     4141 tccgatcact gcgccaccca tcacgtcgcc gtgccccgac gcgtacttgg tcaggctgtg
     4201 ggcaaacagg tccacgtcgt actgcccgtg gttatgcagc cctgcgagcg tattgtcgag
     4261 cagcgtcagc gccccgtgcc tgcgcgccac cgcggtaacg cgctcgagat cggcgacttt
     4321 cagcaccgga ttggtgggcg actcgaacac gatgagtcgc gtcggcgtgg cggccagcgt
     4381 gcgctcgagc gcgtcatggt cggtcaccga cagcatcgtc gcggagacgc cgtaacgacc
     4441 gagcacccgg cgcacgaacg accgcgtcgg tgagtaggat tccgcgaaat acacgacgtg
     4501 gtcgcccgac ttcagcagcg cgagcagcgt cgtggtgatc gacgcgatgc ccgaagccgt
     4561 gagcaggcat gcgtcgcgct gctgcaactc cgcgagcacg agctcgagct gccgcagcgt
     4621 ggggttcccg tcgcgcgaat agtagaagcc atccctctgg ccggaccacg ccttgcgggt
     4681 ctccgccacg tcgtcgaacg tgaacttcac ggactggtaa atcggcgcga cgagggcccg
     4741 gttgtccggc ggcaccggca cgtcaggcag gtgcgtgacg cgcgtgcgct tcttcatgtc
     4801 agctcgaacc cgtctgcgtc gaggtgagcc gggaagctct cgcgccacgc gcgcagcgac
     4861 tgcaggtcga gcaccgccgt ctcgacgcgg tccccggcgc cctcgctcga gacgggctgg
     4921 cccaggaagt cgaggacgac gctgtcaccg gcgtaggcga ctccgttgcc gtcgcggccg
     4981 atgcgattca cgcccgccac gtagcagagg ttctcgatcg cgcgcgcgcg gagcagcgcc
     5041 gaccacgcgt gcgcgcggcg cgccggccag ttcgcgacgt acagcagcag gtcgtagtcg
     5101 ccgcggttcc ggctccagac cgggaagcgc aggtcgtagc agacgagcgg gcagactttc
     5161 cagcccttca cgttgacgac gatgcgccgg tcgcccggcc ggtagtgctc gtgctcgccg
     5221 gccatccgga acaggtggcg cttgtcgtag tgctcgagcg acccgtccgg tcgcgcccag
     5281 acgagccggt tcacgtgccg gtccccgtca cgcacgatca ggctgccggt gatgacgcag
     5341 cccaccgccg ccgcttcctc ccgcatccac ccgacggtcg gaccgtccat cggctcggcg
     5401 agggccgcgg cgtccatgct gaagcccgtc gagaacatct cgggcagcac gacgaggtcc
     5461 gtgtgccccg ccaggccccg gaagtgcgcc gccagtcggt gccggttcgc ctccgggtca
     5521 cgccaggcga ggtccgcctg caccagcgtg acgcgcaagt gctggttcat cactgcaatc
     5581 tcctcaggcg ttcaccagct gcctcgatgg tctctgcact cttggcgaag cagaatcgca
     5641 ccagcgacat gttcaatggt cggctcgaga ataccgagat cgggatcgcg gccacgccga
     5701 tttcccgggt gagccaccgg gcaaactcca cgtccggcag tacgctgacg cgggaatagt
     5761 ccgcgagctg gaaataggtg cccgccgagg gcgtgaaccg cagcgccgtg ccctccagct
     5821 gctggcagaa gtggtcgcgc ttgccctggt agaacccggc aagctcgagg tagtgctgcg
     5881 ggcattcgcg caggaacccg gccagcgccg cctgcatcgg cgtggcgacc gcgaactgca
     5941 cgaactggtg caccttgcgg aactcggcgg tgagcgccgc cggtgcgatg caatagccga
     6001 ccttccagcc ggtcgcatga taggtcttgc cgaacgacga cacgacgaag ctgcgcgcgg
     6061 cgagctcctc gtgttcgagc acgctctcgt ggctgcggcc gtcgaacacc atgtgctcgt
     6121 acacctcgtc ggacagcacc agcgcatccg taccgcgcaa ggcagcggcc agcgcgtcga
     6181 ggtccgcccg ggaccagacg gcaccgctcg gattgtgcgg cgaattgacg atgacgagcc
     6241 gcgtgcgacg cgagatggct gcccgcaccc gttcccagtc caccgcaaac gtcggcggct
     6301 cgagcgggac gcggaccgcg cgtccacccg ccagctgcac cgcgggttcg taggaatcgt
     6361 acgccggctc gagcaggatc acctcgtcgc ccgtccgcac gacggcctgc accgcgcaga
     6421 aaagcgcctc ggtgccgccg gaggtgacgg tcacctcgtc accgggcgac acggtgcgac
     6481 cgtacagcgc acgcaccttg gcggcgatcg cttcgaccag cgcaggccat cccgccatgg
     6541 gcgcgtactg gttgcgcccc tcgcgcatgt gctgcgcgac gagttcgacg aggcg
//
LOCUS       DAOMFD010000109         6580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4255_length_6580_cov_28.961379, whole genome shotgun sequence.
ACCESSION   DAOMFD010000109 DAOMFD010000000
VERSION     DAOMFD010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4255_length_6580_cov_28.961379"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(178..1080)
                     /locus_tag="PL152_07260"
     CDS             complement(178..1080)
                     /locus_tag="PL152_07260"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HQR49118.1"
                     /translation="MSNETEVAVLFADVVGSTRLYELLGDLKARDMVVTCVDLMRAAT
                     ERNRGSVIKTIGDEILAIFPTANDAVNAAGEMQQDIGAHPGLSVQGQHVAIRIGCHFG
                     PVVLENRDIFGASVHTANRMTSQAKAGQIILTSEMVGRLSPEWRAVTRQIDVTPLRGR
                     TEEVELFEVMWQQEDATSMLPSIEVEARTGHRPKRVRLRFKGRDLVLGEGKESLTMGR
                     AEENDIVVQGSPISRIHAKIEIARNRVMLVDQSTNGTFVVAQDGKESFVRRDSLHLVG
                     QGMIGLGKLPDSSAPDAIRYQVED"
     gene            complement(1092..2201)
                     /gene="dusA"
                     /locus_tag="PL152_07265"
     CDS             complement(1092..2201)
                     /gene="dusA"
                     /locus_tag="PL152_07265"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: protein motif:HMM:NF008774.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0002943 - tRNA dihydrouridine synthesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HQR49119.1"
                     /translation="MTDNKEIGDIHHFSARNGECPQFPDRRICVAPMMDYTDRHCRYL
                     HRLVSPAVLLYTEMVTAQAVRHGHVERLLGFDPAESPVALQVGGSDPALLAHAAKLGE
                     QRGYAEINLNVGCPSDRVQEGNFGACLMAEPQLVSDCLRAMLDAVSIPVTVKCRIGID
                     ELDDYGFFDHFVQTVRRSGVDVFIVHARKAILQGLTPKENREIPPLRYETVARLKREH
                     PELTVILNGGLKTVAQVREWWDRCDGVMLGRQAYQEPYLLADLHREFIDRAWTPPCRE
                     EIVERYAAYVERMLHDGHRLPLLLRPIHGLYAGFPGARSWRRFLAEQGNVAGADAQVL
                     RDSLRIVRGASLGRVNPPLYNPAQPVAARDGAAGA"
     gene            complement(2198..3331)
                     /locus_tag="PL152_07270"
     CDS             complement(2198..3331)
                     /locus_tag="PL152_07270"
                     /inference="COORDINATES: protein motif:HMM:NF010039.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HQR49120.1"
                     /translation="MSPGEPPLTLGIEEEYLLVDRETRAVASDPPPQFFADLHERTGG
                     RAFPEFLRSQVEVATPVCRSVGEARAQLRDLRRAVVEEGAKHGLAPIAASSHPFSLTS
                     KQKRTDKERYVALLEEMQGAARRLMVCGLHVHVGFDDDDMRIDLLNQLTYFLPHLLAL
                     SCSSPFWEGERTGLMSFRLMIFNGVPRTGLPEHFTSWGEYRRHTDILVETGIIADTSR
                     IWWDLRPSLRYPTIETRVMDCCTTLEDAISLAALNQCLLRMLYRLRRANQRWRMYSHL
                     LLGENRWRAMRYSYDAGLLDLGRKRIVPFAELLDEIIGLVREDAEALGCVAEVDSLRT
                     ILRRGTSAHRQLAVYEGALAAGASDEEALRAVVDWLVAETAHP"
     gene            complement(3328..4128)
                     /locus_tag="PL152_07275"
     CDS             complement(3328..4128)
                     /locus_tag="PL152_07275"
                     /inference="COORDINATES: protein motif:HMM:NF016873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HQR49121.1"
                     /translation="MPTDHTDHPPASIPLIGRDDPPPFVVLHEHGAAPALVVCDHASR
                     AFPRRMERLGLPVLQTWEHIAWDLGAGEITRGVSHALDAPAVLAGYSRLVVDCNRRPD
                     DPEAFRRESDGWIIPGNQSLDEIERRVRLGCFYDPYHEAIAAMLGGFRARSIVPLFVS
                     VHTFTPQLAGQARPWHVGVLWDKDEETARHMLGGLRAIEGLVVGDNEPYSGRHPGDYT
                     VDHHAESAGLPHVCIEVRQDLLESPAGVERWVRILARLIGGLLAERAG"
     gene            4303..>6580
                     /locus_tag="PL152_07280"
     CDS             4303..>6580
                     /locus_tag="PL152_07280"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HQR49122.1"
                     /translation="MDRSKEPGSPPHGQHEDAPGGTDVLASHTATTGRDLRGSTATQL
                     QAIVDHVKDAILTVDDSGRIETLNSTAERVFGHEEDTVRGRRLDVLIPSLAQHTSLRE
                     ALDELAESLENTQCDLAPRETRGRRHDGTLFDAELGVSKVELDLRNVFIVCLRDTTER
                     KLAEASIRESEARYRTLVENAPEAIVVLDTDLGRFVECNENAVRFFKMTREELLRVGP
                     EEISAPTQADGTPTAGMSRGYVDRALSGEAPCFEWMHRDSLGHDIPCEVRLVRLPSSG
                     RRLIRGSITDITERKRSELLSAGERRVFERITGHADLGVTLEAIAETAERVTPEALCT
                     VSLYDPAPNVLRCVAGRRMPEELLDVLARVEVGPRNGSCAAAIFLQRQVLVAEIARDA
                     LWEHLRPSALAAGMRACWSTPIRSSDGRMLGTVALYFRQPRSPLRRDFDLMSRLTALA
                     GIAIERRRAEEALRRSESRYRELFENVIEGVYRTDVHGRLETVNPALVEMLGFERAED
                     LLAISSTETLYVDPADRERVVGMLHRDGKVHNAEYQLRRRDGRVITVLENARMVRDPD
                     GSVAGYEGTLADISTRKLAELQLAEEKEKAQVTLQCIGDAVLTADAHERVEYLNPVAE
                     QLTGWTTAEAIGRPVAEVFQLLNESTRVPIDSPIVRCLREDRVVDLPEPALLVNRRAQ
                     EISIQDSAAPIRDRSGRVIGAVMVFHDVSQERRLQRALTYQATHDALTGLINRREFEI
                     RLAEALQSAQDNEALNHVL"
BASE COUNT         1043 a         2326 c         2227 g          984 t
ORIGIN      
        1 tcaaggcctg gcatcgcgag atcgacgagg tcggcaagta cctcgaggag tacggtggcc
       61 gactgccggc tgcgttgcgc gccgagtacc agcgcgtgaa gcacgcgctc ggctgaacgg
      121 aaaatgggga cattctccta tttcgcgaat gcgaaatagg agaatgtccc cattttctca
      181 gtcctcgacc tggtagcgga ttgcgtccgg cgcgctcgag tccggcagct tgccgagccc
      241 gatcatgccc tgccccacga ggtgcagcga gtcgcgccgg acgaaggact ccttgccgtc
      301 ctgcgccacc acgaacgtgc cgttcgtact ctggtcgacg agcatcaccc tgttgcgcgc
      361 gatctcgatc ttggcgtgga tgcgcgagat cgggctgccc tgcacgacga tgtcgttctc
      421 ctcggcgcgg cccatcgtca ggctttcctt gccctcgccg agcacgaggt cccggccctt
      481 gaatcgcagc cgcacgcgct tcggccggtg cccggtgcgc gcctcgacct cgatcgacgg
      541 cagcatgctg gtcgcgtcct cctgctgcca catcacctcg aagagttcca cctcctcggt
      601 gcggccgcgc aacggggtga cgtcgatctg gcgcgtcacc gctcgccact ccggcgacag
      661 gcgtccgacc atctccgaag tcaggatgat ctgcccggct ttcgcctggc tggtcatgcg
      721 gttcgcggtg tggaccgagg cgccgaagat gtcgcggttc tcgagcacca ccggcccgaa
      781 gtggcagccg atccggatcg ccacgtgctg gccctggacg gagaggccgg ggtgcgcacc
      841 gatgtcctgc tgcatctcgc cggccgcgtt cacggcgtcg ttcgccgtcg gaaagatcgc
      901 gaggatctcg tcgccgatcg tcttgatcac cgagccgcgg ttgcgctcgg tcgccgcgcg
      961 catcaggtcc acgcacgtga ccaccatgtc gcgcgccttg aggtcgccga gcaactcgta
     1021 gaggcgcgtg ctccccacga cgtcggcgaa caggacggca acttcggtct cgttgctcat
     1081 cggcggtcgg atcaggcccc ggctgcgccg tcgcgggctg cgacgggttg cgcgggatta
     1141 tacagaggcg ggttgacccg tcccaaagaa gcgccacgca cgatgcgcag cgaatcccgc
     1201 aggacttgcg cgtcggcgcc ggccacgttg ccctgctcgg ccaggaagcg ccgccacgag
     1261 cgcgcgcccg ggaagcctgc gtacaagccg tggatcggcc gcagcagcag cggcaatcga
     1321 tgcccatcgt gcagcatgcg ttcgacgtaa gcggcatacc tctcgacgat ctcctcgcgg
     1381 cacggcggcg tccacgcgcg atcgatgaac tcgcggtgca ggtccgcgag caggtaaggc
     1441 tcctggtacg cctgtcggcc gagcatcacg ccgtcgcatc ggtcccacca ctcgcgcacc
     1501 tgcgcgacgg tcttgagccc cccgttcagg atcaccgtga gttccgggtg ctcgcgcttg
     1561 agccgcgcga ccgtctcgta gcgcagcggc gggatttccc ggttctcctt cggcgtgagt
     1621 ccctgcagga tcgccttgcg cgcgtggacg atgaacacgt cgacgccgga acgccgcacc
     1681 gtctgcacga agtggtcgaa gaagccgtag tcgtcgagct cgtcgatgcc gatgcggcac
     1741 ttcaccgtga cggggatgct caccgcatcg agcatcgcac gcaggcagtc gctcacgagc
     1801 tgcggctcgg ccatcaggca cgcgccgaaa ttgccttcct gcacgcggtc gctcgggcag
     1861 ccgacgttga ggttgatctc cgcatagcca cgctgctcgc cgagcttcgc ggcgtgtgcg
     1921 agcagggccg ggtcggagcc gccgacctgc agcgcgacgg gcgactccgc cgggtcgaat
     1981 ccgagcagtc gctcgacgtg cccgtgacgc accgcctgcg cggtcaccat ctcggtgtac
     2041 agcagcaccg ccgggctcac cagccggtgc aggtagcggc agtggcggtc cgtgtagtcc
     2101 atcatcggcg cgacgcaaat ccgccgatcc ggaaattggg gacattcacc atttctcgcc
     2161 gaaaaatggt gaatgtcccc aatttctttg ttgtcggtca tgggtgtgcc gtctcggcga
     2221 ccagccagtc cacgaccgcg cgcaaggctt cctcatcgga cgcgcccgcc gcgagagcgc
     2281 cctcgtacac ggcaagctgc cgatgcgcgc tcgtgccgcg cctcaggatc gtccgcaagc
     2341 tgtcgacctc cgccacgcag ccgagagcct cggcatcctc gcgcaccagc ccgatgatct
     2401 cgtccagcaa ttcagcgaac ggcacgatgc gcttgcgacc gaggtcgagc agtcccgcgt
     2461 cgtacgagta gcgcatcgca cgccagcggt tctcgccgag cagcaggtgc gaatacatcc
     2521 gccagcgctg gttcgcgcgc cgcagccggt acagcatgcg cagcaggcac tggttgagcg
     2581 ccgcaagcga aatcgcgtcc tcgagcgtgg tgcagcagtc catcacccgc gtctcgatcg
     2641 tcgggtagcg caggctcggg cgcaggtccc accagatgcg cgacgtgtcg gcgatgatgc
     2701 cggtctcgac caggatgtcg gtgtgccggc gatactcgcc ccagctcgtg aagtgctcgg
     2761 gcagcccggt gcgcggcacg ccgttgaaga tcatgagccg gaacgacatg agcccggtcc
     2821 gctcgccctc ccagaacggc gaactgcacg acagcgcgag caggtgcggc aggaagtacg
     2881 tcagctggtt cagcaggtcg atgcgcatgt cgtcgtcgtc gaagccgacg tgcacgtgca
     2941 gcccgcacac catcagccgg cgcgccgccc cctgcatttc ctcgagcagc gcgacgtagc
     3001 gctccttgtc cgtgcgcttc tgcttggacg tgagcgagaa cgggtggctc gacgccgcga
     3061 tcggcgcgag cccgtgcttg gcgccctcct cgacgacggc acgcctcaga tcgcggagct
     3121 gggcacgcgc ctcgccgacc gaacggcaga cgggagtggc gacctcgacc tgcgatcgca
     3181 ggaactcggg gaatgcccgg ccgcccgtgc gttcgtgcag gtccgcgaag aactgcggcg
     3241 gcggatcgct cgcgacggcc cgcgtctcgc ggtccacgag caggtactcc tcctcgatgc
     3301 cgagggtcag cggcggctcg cccggactca tcctgcccgc tccgcgagca gaccgccgat
     3361 cagccgcgcg aggatccgca cccaccgctc gacaccggcc ggcgactcga gcaggtcctg
     3421 cctcacctcg atgcacacgt gcggcaatcc cgcgctctcc gcgtggtgat cgacggtgta
     3481 gtcgcccggg tgccgtccgc tgtacggctc gttgtcgccg accaccaggc cctcgatcgc
     3541 gcgcaatccc ccgagcatat ggcgcgccgt ctcctcgtcc ttgtcccaca gcacgccgac
     3601 gtgccacggc cgggcctgcc cggccagttg cggcgtgaag gtgtgcaccg acacgaacag
     3661 cggcacgatc gaccgcgccc ggaatccgcc cagcatggcg gcgatcgcct cgtggtacgg
     3721 atcgtagaag cacccgagtc ggacgcggcg ctcgatctcg tccagcgact ggttgcccgg
     3781 aatgatccag ccgtcgctct cgcggcggaa cgcctccggg tcgtcgggcc ggcgattgca
     3841 gtccacgacc aggcgcgaat agcccgccag cacggccggc gcgtcgagcg catgggacac
     3901 cccgcgcgtg atctcgcccg cgccgaggtc ccaggcgatg tgctcccacg tctgcaggac
     3961 cggcaggccg agccgctcca tgcgacgcgg aaatgcccgg ctcgcgtggt cgcagacgac
     4021 caaagccggt gccgcaccat gttcgtgaag gacgacgaat ggcggcggat cgtcgcggcc
     4081 gatgagcgga atgctggccg gcgggtggtc ggtgtggtcg gtgggcatgg aacggctttg
     4141 cgcagcgtcc ggattgtacc ggatggccct cgaggcccgg gccgaatgcg acctatgtca
     4201 caccggcgca accgggcggg cgcctagtat ccggtacggc ctgcccctgc acccgggcag
     4261 cggccgcgtt gggcgtgggt gtctcggggc acgcatcggg tcatggatcg cagcaaggaa
     4321 ccagggtcgc cgccgcacgg gcagcacgag gatgcgcccg ggggcaccga cgtgctcgcg
     4381 agccacaccg cgacgaccgg ccgcgacctg cgcggcagca ccgccacgca gctgcaggcg
     4441 atcgtggacc acgtcaagga cgcgatcctg acggtcgacg acagcggtcg catcgagacg
     4501 ctgaacagca ccgccgagcg cgtcttcggt cacgaagagg acaccgttcg cggccgccgg
     4561 ctcgacgtcc tgatcccgtc gctcgcccag cacacgagcc tgcgcgaggc cctggacgaa
     4621 ctggccgagt ccctcgagaa cacccagtgc gacctcgcgc cccgcgagac ccggggccgc
     4681 cgccacgacg gcacgctgtt cgacgccgag ctcggcgtga gcaaggtcga gctcgacctg
     4741 cgcaacgtct tcatcgtctg cctgcgcgac acgacggagc ggaagctcgc cgaagcgtcc
     4801 atccgggaga gcgaggcgcg ctatcgcacg ctggtcgaga acgcgccgga agcgatcgtc
     4861 gtcctcgaca ccgacctcgg gcgcttcgtc gaatgcaacg agaacgccgt gcggttcttc
     4921 aagatgacgc gcgaggagct gctgcgcgtc ggacccgagg agatcagcgc gccgacgcag
     4981 gcggacggca cgccgaccgc aggcatgtcg cgcggctacg tcgaccgcgc cctgtccggc
     5041 gaagcgccct gcttcgagtg gatgcaccgc gactcgctgg gccacgacat accctgcgaa
     5101 gtgcggctgg tgcgcctgcc ctcctccggg cgccggctga tccgcggcag catcaccgac
     5161 atcaccgagc gcaagcgcag cgaactgctg tccgcgggcg agcggcgcgt cttcgagcgc
     5221 atcaccggtc atgcggacct cggcgtgacg ctggaagcga tcgccgagac cgccgagcgg
     5281 gtcacgccgg aggcgctgtg cacggtcagc ctgtacgacc ccgctccgaa cgtgctgcgt
     5341 tgcgtcgcgg gccggcgcat gccggaggag ttgctcgacg tgctggcgcg agtcgaggtc
     5401 ggaccccgca acggatcctg tgcggcggcg atattcctgc agcggcaggt gctggtcgcg
     5461 gagatcgcgc gcgacgcgct ctgggagcac ctgcgcccta gcgcattggc cgcaggcatg
     5521 cgggcctgct ggtccacgcc gatccgctcc tcggacgggc gcatgctcgg caccgtggcg
     5581 ctgtacttcc gccagccacg cagcccgctg cgccgggact tcgacctcat gagccgcctc
     5641 accgcgctgg ccggcatcgc catcgagcgc cggcgtgcgg aagaggccct gcgccgcagc
     5701 gagtcgcggt atcgggaact cttcgagaac gtcatcgagg gcgtgtatcg caccgacgtg
     5761 cacggccggc tcgagacggt caatcccgcg ctcgtcgaga tgctgggctt cgaacgggcc
     5821 gaggacctgc tcgcgatctc ctcgaccgag accctgtacg tggatccggc cgaccgcgag
     5881 cgcgtcgtgg ggatgctcca ccgcgacggc aaggtccaca acgccgagta ccagctgcgg
     5941 aggcgtgacg gtcgcgtcat caccgtgctc gagaacgccc ggatggtgcg cgaccccgac
     6001 ggctcggtcg ccgggtacga ggggaccctc gccgacatca gcacgcgcaa gctggccgag
     6061 ctgcagctcg ccgaggagaa ggaaaaggcg caggtgaccc tgcagtgcat cggcgacgcc
     6121 gtgctcacgg ccgacgccca tgagcgcgtc gagtacctga atccggtcgc cgagcagctc
     6181 acgggctgga cgacggccga ggcgatcggg cggcccgttg ccgaggtgtt ccagctgctc
     6241 aacgaatcga cgcgcgtgcc gatcgacagt ccgatcgtgc gctgcctgcg cgaggaccgg
     6301 gtcgtggacc tgcccgagcc cgcactgctc gtgaaccgtc gcgcccagga gatctcgatc
     6361 caggactcgg cggctcccat ccgcgaccgc agcggccggg tgattggcgc ggtcatggtc
     6421 ttccacgacg tgagccagga gcggcggctg cagcgcgcgc tgacctacca ggcgacccac
     6481 gacgcgctca ccgggctcat caatcgccgg gaattcgaga tacgactcgc ggaggcgctg
     6541 cagtcggcgc aggacaacga ggcgctgaac cacgtcctgc
//
LOCUS       DAOMFD010000110         6516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4325_length_6516_cov_38.854821, whole genome shotgun sequence.
ACCESSION   DAOMFD010000110 DAOMFD010000000
VERSION     DAOMFD010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6516
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4325_length_6516_cov_38.854821"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PL152_07285"
     CDS             complement(<1..232)
                     /locus_tag="PL152_07285"
                     /inference="COORDINATES: protein motif:HMM:NF013483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49123.1"
                     /translation="MSSEPDAQALRRDAIVRILRQTAVGRQAELVRLLHKEGLDATQS
                     SVSRDLREIGVVKGADRYLLPAAEDALTPSHFE"
     gene            405..1022
                     /locus_tag="PL152_07290"
     CDS             405..1022
                     /locus_tag="PL152_07290"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR49124.1"
                     /translation="MTDRLLESARRLHALARTGLHFCRDGYDRERYAEIERIAGELLA
                     GDSEPQLERLLQLWAQERGYVTPKVEVRAAVFSEGRVLLVREVADGLWTLPGGWADVN
                     EGPAQAVEKEVEQESGLAVRAVKLAALYDRSRHGHGTSLFHSWKAFFVCEILGGELRG
                     SLETDAAEFFPPDALPPMSLGRSTPAQVLRMREHWLYASLPADFD"
     gene            complement(1049..4819)
                     /locus_tag="PL152_07295"
     CDS             complement(1049..4819)
                     /locus_tag="PL152_07295"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HQR49125.1"
                     /translation="MKRRHVAFYLVAALLLAAPVVLVRHLLYTQRGLEQVLAALAHIE
                     DVHIEVKGARGVIAGPLEFDYVVVDHAAVHVDAYGVRGTPAVLSLLAGRLAIRDASVD
                     RVEVTIKERGPQPDTPVHFLPAWLGIVAPEAVIRQVGVTLKDGTRLHVASVRADLRMT
                     RWRIDVAPFVLQDPAGRVDGEIFLRAGMPLGLRGKLAGRWKLPDDHLYRVSLVMNGRL
                     DRLGVEAALTEPAQIAFAGSALDLEHVPRIVGTLRITDFDGSPWLPAGRLPASSGSIA
                     VDASERGIGFDGTLASHAVGHELVRVRGSATYGDDILHVASLRTWLPKSGASIDASGA
                     VEFAGEAPRVDMRGEWTSLRWPLAGEPLVVSPAGSLRLAGAMPYEFDLRARASGPQLP
                     AADFTAQGSIDREELRVQHLDANMLQGQVSGSGRVSLGTDESWEATVDGRGLDPSTLR
                     RDLSGRVDVAAGISGRGFSATAPWTVRLAKLSGTLNGRALTGRGEISHHDGTYDLTNV
                     RVANAGSHVDVDGRYGPTLDLRFDADLKSLALLDASLQGELSGSGTLRGDRTRPAAKA
                     QLRGRRLKVGDFAAGSASVDVDVDLADHRPSSILVRSSGVTAGRIQFDSVRLEASGLM
                     GEHRVALALISPGSEGGQVPGFEAALAATGSVDVAGRSWHGTLEQASFDFTDGSARLA
                     QPVTLSVGPELASATPLCLVTGDARLCAEGEWHATPGSWRVLYSAQDWPLRRLLTSLL
                     GRREFDGLLQLSGWAEQQPGHDWVGGTAVVIDHPTVDIRRNRFRSDRVEIGGGRLDLY
                     ADESRVRATADLEMAAGTRLQGEVSAPRERGRPLADSPLTGEVRAESSVLTVMPLFVP
                     EIDHSEGRLDADVHVGGTLGDPRFDGEFHLRDGRLDLYRTNLSLTQANVDGQFAGDTL
                     TFEGRATTPKGPVSLSGRFTWPDGVMTGSMRLTGQDLLVADTPEYRVQASPDLTIRAG
                     RDGFDVTGQIVIPWARISPKDLSTSVATSPDERIVGARAEEASAPSTKRRVHASVKVT
                     LGDDVRVETYGLKAHLGGEVLVTSDPGEEPRGEGAIRVLEGEYKAFGVYVKITKGVLS
                     YHDAPLALPTLDLVAEREIKDEDVKVAVNVRGRIDKPFVTVSSQPAMPSNEALSYLLT
                     GRSINTLQSNEAVSVNKAAESLAVSGGGLLLGGLGSRLGLDEVSVEGTRDKDTQVVLG
                     KFLSPKLFVSYGVSIAEAINTIKLRYTMNPRWALKAEAGLAQSADVEFKIER"
     gene            complement(4819..>6516)
                     /locus_tag="PL152_07300"
     CDS             complement(4819..>6516)
                     /locus_tag="PL152_07300"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HQR49126.1"
                     /translation="AADTIRLEIDGVDRAMADNVRAYLSLSRYLQRTDLTDDQVRRLA
                     DRAVDEASDALRPYGYYDPQVRSRTVRDAPGWVVRLKVKPGEPVVMREVQVDIVGAGQ
                     DDTEIRNVVTSDPLRTGVRLDHPAYEKFKTDLVRTALEQGYLDAKLTRHELDVDPQEH
                     AADVRLVLDTGGRYRFGKVTIEQDAIDPEHLQGFIRFTEGKPFSATLVRSTQFALEDS
                     FYFSSVTVTPGPRDPETLTVPITIRAERIKRDRYAASVGFGTDTGPRGQFAWDRRLVN
                     EAGHRWRLQTTLSKVLQDVTASYIIPIGDPSLEKLEFSAGYVSETLGGLESERYEVAA
                     GITQVMGSWQRVLFAQLSQELTKYPGNQDTRSLLLIPGISFATQPPNFLTGWDRNAAY
                     YFELSGSPASLGSDASWLRFYARGERVWAIDQGPWFLRLRGEFGTTWTTNFSQVPGSQ
                     RFFAGGDNSVRGFALDSLSPRDPDAPPGSGNKGVGGKNLVVASIGVERDLPHDLRGAL
                     FFDTGNAFDDWNTPLEYSVGVGLRYRLPMLMIGFDVAQALSEPDKRPRLHLNITQVL"
BASE COUNT          941 a         2305 c         2291 g          979 t
ORIGIN      
        1 cctcgaaatg gctcggcgtg agcgcgtcct cggcggcggg cagcaggtag cggtccgcgc
       61 ccttgaccac gccgatctcg cgcaggtcgc ggctcacgct cgactgcgtg gcgtcgaggc
      121 cctccttgtg cagcaggcgc accagctccg cctgccggcc gaccgcggtc tgccggagga
      181 tgcggacgat ggcgtcccgc cgcagggcct gcgcgtccgg ttcgctggac aaggtcgggc
      241 ttccatgttc gtgaataaaa acgagcgcaa tgtgcataaa tatgcaaaac atgtcaagtg
      301 ccgcgggcgg ggccgcgccg cgcgctccgg ccgggcaggg aggcgcagcg ccgcaggctg
      361 ggcgagaatg gcgggcaccg ccgccgaccg cccggggaca cgccatgacc gaccgcctgc
      421 tcgagagcgc ccgccgcctg cacgcgctgg cccgcaccgg cctgcacttc tgccgcgacg
      481 ggtacgaccg cgagcgatac gcggaaatcg agcggatcgc gggcgagctg ctggcgggcg
      541 actcggagcc gcagctcgaa cggctcctgc agttgtgggc ccaggagcgt ggctacgtca
      601 cgcccaaggt ggaagtgcgg gccgcggtct tcagcgaggg gcgcgtgctg ctggtccgcg
      661 aggtcgccga cggcctgtgg accctgcccg gcggctgggc cgacgtcaac gagggccctg
      721 cgcaggccgt cgagaaggaa gtcgagcagg agtccgggct cgcggtgcgc gcggtgaagc
      781 tcgcggccct gtacgaccgc agccgccacg ggcacggcac gtcgctcttc cattcgtgga
      841 aggccttctt cgtctgcgag atcctcggcg gggagttgcg cggcagcctc gagaccgatg
      901 cggcggagtt cttcccgccc gacgcgttgc cgccgatgtc actggggcgc agcaccccgg
      961 cccaggtcct gcgcatgcgc gagcactggc tctacgcttc gctcccggcc gacttcgact
     1021 aggcgagcgg gctgcgcagc ccgagggatc aacgctcgat cttgaactcc acgtccgcgc
     1081 tctgcgcgag gccggcctcg gccttgagcg cccagcgcgg attcatggtg tagcgcagct
     1141 tgatcgtgtt gatcgcctcc gcgatcgaga ccccgtagct cacgaagagc ttcggcgaga
     1201 ggaatttccc gagcacgacc tgggtgtcct tgtccctggt gccctccacg gacacctcgt
     1261 cgagcccgag ccgcgagccg aggcctccca gcagcaggcc gccgccgctc acggcgaggc
     1321 tctcggccgc cttgttgacc gacacggcct cgttcgactg cagggtgttg atcgagcgcc
     1381 cggtgagcag gtaggagagc gcctcgttgc tcggcatggc gggctgcgac gacaccgtga
     1441 cgaacggctt gtcgatgcgc ccgcgcacgt tgactgccac cttcacgtcc tcgtccttga
     1501 tctcgcgctc ggcgacgagg tcgagcgtcg gcagcgcgag cggggcgtcg tggtacgaaa
     1561 gcactccctt ggtgatcttg acgtacacgc cgaacgcctt gtattccccc tcgagcacgc
     1621 ggatcgcacc ctcgccgcgt ggttcctcgc ccggatcgga ggtgaccagc acctcgccgc
     1681 cgaggtgcgc cttgagccca taggtctcga cgcgcacgtc gtcgccgagc gtgaccttga
     1741 cggaggcgtg cacgcgtcgc ttcgtcgacg gcgcggaagc ttcctctgca cgagcgccca
     1801 cgatgcgctc gtccggcgag gtggccacgg acgtgctcag gtccttcggc gagatccggg
     1861 cccaggggat cacgatctgg cccgtcacgt cgaagccgtc gcgcccagcc ctgatcgtga
     1921 ggtccgggga cgcctgcacg cggtactccg gcgtgtcggc gacgagcagg tcctggcccg
     1981 tcagccgcat gcttcccgtc atgactccat ccggccaggt gaaccggccg gagagcgaca
     2041 ccggaccctt cggcgtcgtc gcgcgacctt cgaacgtcag cgtgtcgccg gcgaactgcc
     2101 cgtcgacatt ggcctgcgtc agcgacaggt tggtccggta caggtcgagc cgcccgtcgc
     2161 ggaggtgaaa ttcgccgtcg aaccgcgggt cgccgagcgt gccgccgacg tgcacgtccg
     2221 cgtcgaggcg gccttcggag tggtcgatct ccgggacgaa cagcggcatg accgtgagca
     2281 cggacgactc ggcgcgcacc tcgcccgtga gcggcgagtc ggcgagcggc cggccgcgtt
     2341 cgcgcggcgc cgacacctcg ccctgcagcc gcgtgccggc cgccatctcg agatcggcgg
     2401 tcgcgcgcac ccggctctcg tcggcataca ggtccaggcg cccgccgccg atctccacgc
     2461 ggtccgaccg gaagcgattg cggcggatgt ccacggtggg atggtcgatc acgaccgcgg
     2521 tcccgccgac ccagtcgtgc cccggttgct gctcggccca gccactcagc tgcaacaggc
     2581 cgtcgaactc cctgcgcccc agcagcgacg tgagcagccg gcgcagcggc cagtcctggg
     2641 cgctgtagag cacccgccac gaccccggcg tcgcgtgcca ttcgccttcc gcgcacaagc
     2701 gcgcatcgcc ggtcacgagg cagagcgggg tcgcgctggc aagctcgggt ccgacgctta
     2761 gcgtcacggg ctgggcgagc cgggcgctgc cgtcggtgaa gtcgaatgac gcctgctcga
     2821 gcgtgccgtg ccaggaacgc cccgcgacgt cgacgctccc ggtcgcggcg agcgcggctt
     2881 cgaaaccggg gacttgcccg ccctcgctgc ccggggagat cagcgcgagc gcgacgcgat
     2941 gctcgcccat gagtccactg gcctcgaggc gcacggagtc gaactggatc cggccagcgg
     3001 tcacgccgct ggatctgacg agaatgctcg acggccggtg gtcggcgagg tccacgtcca
     3061 cgtcgacgct ggcggagccc gcggcgaagt cgccgacctt cagtcgacgc ccccgcagct
     3121 gggctttcgc ggccgggcgg gtcctgtcgc ctcgcagcgt gccactgccg gagagttcgc
     3181 cctgcagcga ggcgtccagc agcgcgaggg acttcaggtc cgcatcgaag cgcaggtcga
     3241 gtgtcgggcc gtagcggccg tccacgtcca cgtgcgagcc cgcgttggcg acgcgcacgt
     3301 tcgtcaggtc gtacgtgccg tcgtggtggg agatctcgcc gcgacccgtc aatgcccgtc
     3361 cattcagcgt gcccgacagc ttcgccaggc gcacggtcca cggtgccgtg gcggagaatc
     3421 cgcgaccgct gatcccggcc gcgacgtcga cgcgaccgct caggtccctg cgcagcgtcg
     3481 acgggtcgag gccgcggccg tccaccgtcg cttcccagga ttcgtccgtg cccagggaga
     3541 cgcggcctga tcccgacacc tgcccttgca gcatgtttgc gtcgaggtgc tgcacgcgca
     3601 actcctcgcg atcgatgctc ccctgcgcgg tgaaatccgc cgcgggcagt tgcgggccgc
     3661 tggctcgagc gcgcaggtcg aactcgtacg gcatcgcgcc cgcgagccgc agcgagcctg
     3721 ccgggctcac gacgagcggt tcgcccgcga gtggccaccg cagcgatgtc cattcgcccc
     3781 gcatgtcgac gcgcggcgcc tcgccggcga actcgaccgc cccggacgcg tcgatcgacg
     3841 cgcccgactt cggcagccac gtgcgcagtg aagcgacatg gaggatatcg tcgccatagg
     3901 tcgccgagcc gcgcacgcgg accagctcgt ggcccaccgc gtgcgatgcc agcgtgccgt
     3961 cgaaaccgat gccgcgctcg cttgcgtcca ccgcgatcga tccgctgctc gcgggcaggc
     4021 gacctgccgg cagccagggc gaaccgtcga aatcggtgat gcgcagggtg cccacgatgc
     4081 ggggaacgtg ctcgaggtcc agggcgctgc ccgcgaaggc gatttgcgcc ggctccgtga
     4141 gcgccgcctc gacgccgagc cggtccagcc tcccgttcat gacgagcgag acgcggtaga
     4201 ggtggtcgtc cggcagcttc cagcggccgg ccagcttgcc ccgcaggccc agtggcatcc
     4261 cggcgcgcag gaagatctcg ccgtccacgc gaccggccgg gtcctgcagc acgaacggcg
     4321 cgacgtcgat gcgccagcgc gtcatccgca ggtcggcgcg tacgctcgcc acgtggaggc
     4381 gcgtgccgtc cttcagcgtc acgccgacct gccggatgac tgcctcgggg gcgacgatgc
     4441 cgagccacgc cggcaggaaa tggaccggcg tgtcgggctg gggcccgcgc tccttgatcg
     4501 tcacttcgac tcgatcgacg ctggcgtcgc ggattgcgag ccgacccgcg agcaggctca
     4561 ggaccgcggg cgtgccgcgg acaccgtagg cgtcgacgtg caccgccgca tgatccacga
     4621 cgacgtagtc gaactcgagc gggccggcga tcacgcccct cgcgcctttc acctcgatgt
     4681 gcacgtcctc gatgtgagcg agcgccgcga gcacctgctc gagtccccgc tgggtgtaca
     4741 gcaggtggcg caccagcacg accggcgccg cgagcagcag cgcggcgacc aggtagaacg
     4801 cgacgtggcg gcgcttcatc acaacacctg cgtgatgttg aggtgcagcc gcggccgctt
     4861 gtcgggctcc gacagcgcct gcgcgacgtc gaatccgatc atcagcatcg gcagccggta
     4921 gcgcagcccg acgccgaccg agtactcgag cggcgtgttc cagtcgtcga acgcgttgcc
     4981 ggtgtcgaag aacagcgcgc cgcgcaggtc gtgcggcagg tcgcgctcga ctccaatgct
     5041 ggcgacgacc aggttcttgc cgccgacgcc cttgttgccg ctgccaggcg gggcgtcggg
     5101 gtccctgggg ctcagtgaat cgagcgcgaa gccgcggaca ctgttgtcgc cgccggcgaa
     5161 gaagcgctgc gaacccggca cctgcgagaa gttggtggtc caggtcgtgc cgaactcgcc
     5221 gcgcaaccgc aggaaccacg ggccctggtc gatcgcccag acgcgctcgc cgcgcgcata
     5281 gaatcgcagc cacgaggcat cggatccgag cgatgccggg ctgccggaca gctcgaagta
     5341 atacgctgcg ttgcgatccc agccggtcag gaagttcggc ggctgggtgg cgaagctgat
     5401 gccggggatc agcagcagcg agcgcgtgtc ctggttgccg ggatacttgg tgagttcctg
     5461 gctcagctgc gcgaacagca cccgctgcca gctgcccatc acctgcgtga tccccgccgc
     5521 gacctcgtag cgctcgctct cgagcccgcc gagcgtctcg ctgacgtacc cagccgaaaa
     5581 ttccagcttt tcgagcgagg ggtcgccgat cgggatgatg tagctggcgg tgacgtcctg
     5641 cagcaccttc gacagcgtcg tctgcagccg ccagcgatgg cccgcttcgt tcacgagccg
     5701 ccggtcccac gcgaactggc cgcgcggccc ggtgtccgtg ccgaagccga cgctcgccgc
     5761 gtagcggtcg cgcttgatgc gctcggcgcg gatcgtgatc ggcacggtga gcgtctcggg
     5821 gtcgcggggg cccggcgtca ccgtgacgct cgagaagtag aagctgtcct ccagcgcgaa
     5881 ctgcgtgctg cgcaccagcg tcgcggagaa aggcttgcct tcggtgaagc ggatgaagcc
     5941 ctgcaggtgc tccggatcga tcgcgtcctg ctcgatggtc accttgccga agcggtagcg
     6001 gccgccggtg tcgagcacga gccgcacatc cgcagcgtgc tcctgcggat cgacgtccag
     6061 ctcgtgccgg gtcagtttgg cgtcgaggta accctgctcg agcgcggtgc ggaccaggtc
     6121 cgtcttgaac ttctcgtagg ccgggtgatc gaggcgaacc ccggtgcgca gcggatcgct
     6181 ggtgacgacg ttgcggatct cggtgtcgtc ctgcccggct ccgacgatgt cgacctgcac
     6241 ctcgcgcatc acgaccggct cgcccggctt gaccttcagg cgcacgaccc agccaggtgc
     6301 gtcgcgcact gtccggctgc gcacctgagg gtcgtagtac ccgtatgggc gcaaggcgtc
     6361 gctcgcttcg tcgaccgccc ggtcggcgag ccggcggacc tggtcgtccg tgaggtccgt
     6421 acgctgcagg tagcggctga gcgagagata ggcgcggacg ttgtcggcca tcgcgcggtc
     6481 gacgccgtcg atctcgaggc ggatcgtgtc ggcggc
//
LOCUS       DAOMFD010000111         6474 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4374_length_6474_cov_35.218570, whole genome shotgun sequence.
ACCESSION   DAOMFD010000111 DAOMFD010000000
VERSION     DAOMFD010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6474)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6474
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4374_length_6474_cov_35.218570"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1069)
                     /locus_tag="PL152_07305"
     CDS             complement(<1..1069)
                     /locus_tag="PL152_07305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HQR49127.1"
                     /translation="MLTHVLFFLLGLGALVVGAEALVRGAAKIALSLGISPLVVGLTI
                     VAMGTSSPEIAVSVGAVMNGSTDLAVGNVVGSNIFNVLFILGVSAVITPLVVHSQIIR
                     QEVPIMIGASVILAVMTLDGLLGRVDSALLLALMVGYVVFLVRQSRAETAEIRDEFEG
                     AAERRDGGWDDHWAVQLLLIVAGLALLVLGSDWLVDAAVAFARAVGVSDLVIGLTIVA
                     AGTSMPEVATSIMAAVRGERDIAVGNVIGSNTFNILGCLGISGVVSSSGLGIAPAVLN
                     FDLWVMLAVAISCLPVFLFGRQIGRRRGLLFLAYYAAYAAYLVMGAQQHDALDEFSAV
                     MLGFVLPITVVTLVAMVVRDQA"
     gene            complement(1122..2267)
                     /locus_tag="PL152_07310"
     CDS             complement(1122..2267)
                     /locus_tag="PL152_07310"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HQR49128.1"
                     /translation="MDPRDGESEAVAAPELLAGGLEAYGRMWRMIEGARHCVDLETYI
                     YRQGAVGDRFRQLLAAAAARGVRVRVLIDAYGSNELPDRYFDALERAGGEVRRFNPRR
                     MLRFSFRNHRKLLRCDAGAVVGGLNVGDEYDGDGVSQGWRDFAVAVEGPVVGPLADSF
                     ERMWQLASFGRESVRSFWRNRPRWIPVGSRSEQLLLSGPGCPTSELRRTLATDIRGAR
                     SFRAWAAYFVPSRRIAVAIRSVARRGDAQVVLGARSDVPVSRWASERLFARFLRAGVR
                     IHDYLPQIMHAKVLIVDDLVYVGSANLDVRSLRINYELLLRVRSSTLAERLREQFGLD
                     VARSEEVLLAEWRRRRRWWRVLRSYLAWLLIVRVDPYVATRKLQSLG"
     gene            complement(2387..2731)
                     /locus_tag="PL152_07315"
     CDS             complement(2387..2731)
                     /locus_tag="PL152_07315"
                     /inference="COORDINATES: protein motif:HMM:NF014534.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPF/RaiA family ribosome-associated protein"
                     /protein_id="HQR49129.1"
                     /translation="MKIDVQARRFTLTHGLRAAVQREIERLAQAAGPRITRISVRLFD
                     VNGTRGGLDKGCLVHAHFDDGRSIVGTDVDSDLYRSVNSAFDKLLRSHRSSVSRRQAR
                     RRTLLRPTLVTA"
     gene            2826..3734
                     /locus_tag="PL152_07320"
     CDS             2826..3734
                     /locus_tag="PL152_07320"
                     /inference="COORDINATES: protein motif:HMM:NF015431.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HQR49130.1"
                     /translation="MSESPQTAVRAPVRINYKHLQYFHAVVREGSVSGAARRLHVAPQ
                     TVSAQVRSLERSLGRQLFERVGRRMVLTPDGEVALDYADSIFTLGQELRGVMAGRVEP
                     RRLVLRLGVTDSVPKLLMARVLDPLFERHRDRLELSCAEAGAADLLGRLASHQLDAVL
                     AEVPVPPHLARTLRSRLVLESGLSFMATRRLKAASRVPFPDCLAELPLLNWPADTTAG
                     SVVDQWLADHALQPRVAGRCDDSALMKVLAQRGLGVIGVPTSIEKDVARQFDLVVLGR
                     VPQLRQPLYLIRPAGRRPHPLLDAIG"
     gene            complement(3771..4472)
                     /locus_tag="PL152_07325"
     CDS             complement(3771..4472)
                     /locus_tag="PL152_07325"
                     /inference="COORDINATES: protein motif:HMM:NF013570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HQR49131.1"
                     /translation="MTDWLFHRDACLKCCTARVVDASPDGVVLDRTVFYPLGGGQPGD
                     TGALKAIDGREWRVTDTRKGDAGRILHRIAQGAPPPAPGMELEAIIDWDRRYLHMRMH
                     TGLHLLGSILRFGVTGGQIAADRSRLDFDTQEEIDPQRVNAALQDLVSADHSVSCRWI
                     TDQELDRQPELVRTLSVQPPRGAGRIRLLEIAGTDLQPCGGTHVRSTGEVGRLVVTRV
                     ENKGRHNKRVYVGFS"
     gene            complement(4516..6276)
                     /locus_tag="PL152_07330"
     CDS             complement(4516..6276)
                     /locus_tag="PL152_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0051139 - metal cation:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter-2 (CPA2)
                     family protein"
                     /protein_id="HQR49132.1"
                     /translation="MTLTGQVAVVLAATVIAIPLFRRLKLSAVLGYIAAGLVIGPWVL
                     GVFTDVERILNFAEFGVVLLLFVIGLELQPSRLRVMRRAVFGGGILQVAITSAGLAVI
                     ARLAGQDWVASAIIGFALSLSSTALILQVLAERGELTTRHGRSAFAILLFQDLAIMPV
                     LLILPLIAGTAAQHISWRTIVLPVATLALVVGAGRYLLRPVLRAIAGTGVQEAFTAAA
                     LLVVIGTAVLFQAAGLSMALGAFIAGVLLADSEYRHELEADIEPFKGLLLGLFFIAVG
                     MSANLGLLVEHPLQVAALTVAFLGAKTLLVWLAARMARHDHATAWRLGVALAGGGEFA
                     FVLFALATRNRLIDPDTSDLLVLVVTASMGLAPVLMALADRIAGRFAAPAPAPVFDQI
                     EPQEPRVLIAGFGRVGQIVARVLRARRIPFTALEVSPAQVDFVRRFGNKLYYGDATRL
                     EMLRAAGADRAEVLVLAIDDVEASVRTAELVRRHFPRTRVIGRARNRQHAFRLMDAGV
                     ASITRETFASSLEMAQSTLVALGTAAETAAAQVRRFREHDEETLRAQAAVKDDEERFV
                     ATTQASARQLETLFEADAAT"
BASE COUNT         1084 a         2253 c         2227 g          910 t
ORIGIN      
        1 gcgcctggtc gcgcaccacc attgccacca gcgtgacgac ggtgatcgga agcacgaagc
       61 ccagcatcac ggcggagaac tcgtcgagcg catcgtgctg ctgcgcgccc atgaccaggt
      121 acgccgcata ggccgcgtag taggcgagga acagcaggcc gcggcggcgg ccgatctgcc
      181 ggccgaacag gaacaccggc aggcaggaga tggccaccgc cagcatcacc cagaggtcga
      241 agttgagcac cgcgggcgcg atgccgaggc cgctggagct caccacgccc gagatcccga
      301 ggcagccgag gatgttgaag gtgttgctgc cgatcacgtt gccgacggcg atgtcgcgct
      361 cgcctcgcac cgcggccatg atcgaggtcg ccacctcggg catcgaggtg cccgcggcga
      421 cgatggtgag gccgatgacc aggtcgctca cgcccactgc gcgcgcgaag gccaccgccg
      481 cgtccaccag ccagtccgag cccagcacca gcagcgcgag cccggccacg atcagcagca
      541 gttgcaccgc ccagtggtcg tcccagccgc cgtcgcgtcg ctccgcggcg ccctcgaact
      601 cgtcgcggat ctcggcagtc tcggcgcgcg attgccggac caggaacacg acgtagccca
      661 ccatcagcgc cagcagcagc gcggaatcga cccgacccag caagccgtcg agcgtcatca
      721 ccgcgaggat caccgaggcg ccgatcatga tcggcacctc ctggcggatg atctgcgagt
      781 gcacgaccag cggcgtgatc acggcgctga cgccgaggat gaacagcacg ttgaagatgt
      841 tgctgccgac cacgttgccg accgcgaggt cggtgctgcc gttcatcacc gcgcccacgg
      901 agaccgcgat ctcgggtgag ctggttccca tcgcgacgat ggtgaggccc acgaccagcg
      961 gcgagatgcc gagcgacagg gcgatcttcg cggcgccgcg caccagcgcc tcggcgccga
     1021 ccaccagcgc ccccaggccg agcaggaaga acaggacgtg ggtgagcatt gcagcgacgg
     1081 cggtgtccgg agggccagcg cgaattctag cggcgtccgg tctacccgag cgactgcagc
     1141 ttgcgggtcg cgacgtaggg gtcgacgcgg acgatcagca gccacgccag gtatgagcgg
     1201 agcacgcgcc accagcgccg ccggcgccgc cactcggcca gcagcacctc ttcggaccgc
     1261 gccacgtcga gcccgaactg ctcgcgcagc cgctccgcga gcgtcgagga gcggacgcga
     1321 agcaacagct cgtagttgat gcgcaggctg cgcacgtcga ggttggccga cccgacgtag
     1381 acgagatcgt cgacgatgag caccttggcg tgcatgatct gcggcaagta gtcgtggatg
     1441 cgcacgcccg cccgcaggaa ccgcgcgaaa agccgttcgc tggcccagcg agacacgggg
     1501 acgtcgctgc gggcaccgag cacgacctgg gcgtcgccgc ggcgggcgac cgagcggatc
     1561 gccacggcga tgcgtcgcga cggcacgaag tacgctgccc aggcgcggaa gctgcgcgcg
     1621 ccgcggatgt cggtcgccag cgtccgtcgc agctcggacg tcgggcagcc gggacccgag
     1681 agcagcaact gctcgctcct ggatcccacc gggatccagc ggggccggtt ccgccagaag
     1741 ctgcgcaccg actcccggcc gaacgaggcg agttgccaca tgcgctcgaa gctgtcggcc
     1801 agtgggccga ccaccgggcc ctcgaccgca acggcgaagt cgcgccaccc ctggctgacc
     1861 ccgtcaccgt cgtattcgtc gccgacgttg aggccgccga ccaccgcgcc tgcgtcgcag
     1921 cgcagcagct tgcggtggtt gcgaaagctg aaccgcagca tccgcctcgg gttgaatcga
     1981 cggacttcgc cgccggcccg ctcgagcgcg tcgaaatacc ggtccggcag ctcgttcgag
     2041 ccgtaggcgt cgatgagcac gcggacgcgg acgccccgcg ccgccgccgc tgccagcagt
     2101 tgacggaacc ggtcgccgac cgcgccctgc ctgtagatgt aggtctcgag atcgacgcaa
     2161 tgccgggcgc cttcgatcat gcgccacatg cgcccatagg cctcgagtcc gcccgccagc
     2221 agctccggcg ccgccactgc ctccgactca ccatcacgcg ggtccatgcc gcaatggtgc
     2281 cacctcgatg tcgccgcgcg cgaggctgcg tggaaagggg accaccgggt cgcaccgccc
     2341 ggtgggtgat gacgatgatc cggtgggggc gcgacccgat ggtcgatcag gccgtgacga
     2401 gcgtcgggcg cagcagggtg cgacgccggg cctggcgacg cgacacgctg gagcgatggc
     2461 tgcgcagcag cttgtcgaag gccgagttca ccgagcggta gagatccgag tcgacgtccg
     2521 tgcccacgat cgaccggccg tcgtcgaagt gcgcgtgcac gaggcagccc ttgtccaggc
     2581 cgccacgcgt gccgttcacg tcgaagaggc gcaccgagat gcgcgtgatg cgtggacccg
     2641 cggcctgcgc gaggcgctcg atctcgcgtt gcaccgcggc tcgcaatccg tgggtgaggg
     2701 tgaatctccg ggcctgaacg tcgatcttca tgttcatcgc ctcctgcatc gatggggcag
     2761 atggtgccga ggctcgcgtg caaataaaat aagaactatc ggcaacaatc atccgaaaaa
     2821 acctgatgag tgaatcaccc caaaccgccg tccgcgcgcc cgtgcggatc aactacaagc
     2881 acctgcagta cttccacgcc gtggtgcggg aagggtcggt gagcggggcg gcgcgtcgcc
     2941 ttcacgtcgc gccgcagacc gtgagcgcgc aggtccgcag cctcgagcgc tcgctcggtc
     3001 gccagctctt cgagcgcgtc gggcgcagga tggtgctcac gccggacggc gaggtcgcgc
     3061 tcgattacgc ggactcgatc ttcacgctcg gccaggagct gcgaggggtg atggccggcc
     3121 gcgtggagcc gcgccgactc gtgctgcggc tgggcgtcac cgacagcgtg ccgaagctgc
     3181 tcatggcgcg cgtgctcgac ccgctgttcg agcggcatcg cgaccgcctc gagctcagct
     3241 gcgccgaggc cggggccgcc gatcttctcg gtcgcctggc gtcgcaccag ctcgacgcag
     3301 tgctggccga ggtgccagtg ccgccgcacc tggcccggac gctgcgcagc cggctcgtgc
     3361 tcgagtcggg gctcagcttc atggcgacgc ggcggctcaa ggccgcgtcg cgcgtcccgt
     3421 ttccggactg cctggccgaa ctgccgctgc tcaactggcc ggccgacacg acggcgggca
     3481 gcgtggtcga ccagtggctg gcggatcatg cgctgcagcc ccgcgtcgcc gggcgctgcg
     3541 acgacagcgc gctcatgaag gtgctggcgc agcgcggact cggcgtgatc ggagtgccga
     3601 cttcgatcga gaaggacgtc gcccggcagt tcgacctcgt cgtgctcggg cgcgtgccgc
     3661 agcttcgcca accgctctac ctgatccgcc ccgccggccg tcggccccac cccctgctcg
     3721 atgcgatcgg gtgaagggga tgctgcacgc gttgctcgtg accttgctcg tcagctgaac
     3781 ccgacgtaga cgcgcttgtt gtgtcgaccc ttgttctcga ccctggtcac cacgagtcgg
     3841 ccgacttctc ccgtcgagcg cacgtgcgtc ccgccgcagg gctggaggtc cgttccggca
     3901 atctcgagga ggcggatccg cccggcgccg cgcggcggct ggaccgacaa cgttcgcacc
     3961 agttccggct ggcggtcgag ttcctgatcg gtaatccagc ggcagctgac cgaatggtcc
     4021 gcggatacga gatcctgcag tgccgcgttc acgcgctgcg gatcgatctc ctcctgcgtg
     4081 tcgaagtcga gacggctccg gtccgcagca atctgtccgc ccgtgacgcc gaagcgcagt
     4141 atcgagccga gcaggtgcag gccggtgtgc atgcgcatgt gaaggtagcg acgatcccag
     4201 tcgatgatcg cctccagttc catgccgggg gccggtggcg gtgcgccttg cgcgatcctg
     4261 tgcaggatgc ggccggcgtc gcccttgcgc gtgtcggtca cacgccactc gcgaccgtca
     4321 atcgccttga gcgcacccgt gtccccgggc tggccgccgc ccagcggata gaagaccgtc
     4381 cggtcgagga cgactccgtc cggcgaggcg tcgacgacgc gcgccgtgca gcacttcagg
     4441 caggcgtccc ggtgaaatag ccaatcggtc attaccggcc ccgtggatcc tgttgcgtca
     4501 cccggtcgtt catggtcacg tggctgcgtc ggcttcgaag agcgtctcga gctgcctggc
     4561 cgacgcctgg gtcgtcgcga cgaacctctc ctcgtcgtcc ttgacggcag cctgcgcgcg
     4621 cagagtctcc tcgtcgtgct cgcggaatcg tcgcacctgt gccgcagcgg tctccgcagc
     4681 ggtgcccagc gccaccagcg tcgactgcgc catctccagg ctcgacgcga acgtctcgcg
     4741 cgtgatcgac gcgacgcccg catccatcag ccggaacgcg tgctggcggt tgcgtgcccg
     4801 gccgatcacc cgtgtccgcg gaaaatgccg tcgcacgagt tcggccgtgc gcacgctcgc
     4861 ctcgacatcg tcgatggcga gcacgagcac ctcggcgcgg tccgccccgg cggcgcgcag
     4921 catctccagc cgggtggcgt cgccgtaata cagcttgttg ccgaaacggc gcacgaaatc
     4981 gacctgcgcc ggactcacct cgagcgcggt aaacggtatg cggcgggcac gcaggactcg
     5041 cgcgacgatc tggccgacgc gaccgaagcc cgcgatcagc acgcgcggtt cctgtggctc
     5101 gatctggtcg aacacgggcg cgggtgcggg cgcggcgaag cggccggcga tacggtcggc
     5161 gagcgccatc agcaccggcg cgagacccat cgacgccgtg acgaccagca ccaggaggtc
     5221 ggatgtgtcg ggatcgatca ggcgatttcg ggtggcgagt gcaaagagca cgaaagcgaa
     5281 ctcaccacca ccggcgaggg ccacgcccag gcgccaggcc gtggcgtgat cgtggcgtgc
     5341 catccgcgcc gcgagccata ccagcaatgt cttcgcgccc aggaacgcga cagtcagggc
     5401 agcgacctgc agcgggtgtt cgaccagcaa gccgaggttg gcggacatgc cgaccgcgat
     5461 gaagaacagt ccgagcagca gccccttgaa tggctcgatg tcggcctcca gttcgtgccg
     5521 gtattcgctg tcggcgagca gcactcctgc gatgaatgcg cccagcgcca tcgagaggcc
     5581 ggccgcctgg aacaacacgg ccgtgccaat cacgacgagc agggcggctg cggtaaacgc
     5641 ttcctgcacg cccgtcccgg caatggctcg cagcaccggt cgcaacaggt agcggccggc
     5701 acccaccacc agtgccagcg tggccaccgg cagcacgatc gtgcgccatg aaatgtgttg
     5761 ggctgcggtg ccggcgatga gcggcaggat cagcagcacc ggcatgatcg ccaggtcctg
     5821 gaacagcagg attgcaaacg ccgagcggcc gtgccgagtc gtcagttcgc cgcgctcggc
     5881 gagcacctgc aggatcagcg ccgtcgacga cagcgacagg gcgaagccga tgatggccga
     5941 cgcgacccag tcctggccgg ccaggcgtgc gatcacggcg agtccggccg aggtgatcgc
     6001 gacctggagg atgccgccgc cgaagacggc gcggcgcatc acccggagac gcgatggctg
     6061 caattcgagc ccgatcacga acagcagcag cacgacgccg aactccgcga agttcaggat
     6121 cctctcgaca tcggtgaaca cgcccagcac ccatgggccg atgacgaggc ccgcggcgat
     6181 gtagccgagg accgcgctca gcttgaggcg gcggaacagc ggaatcgcga tgaccgtggc
     6241 ggcgagcacc acggcgacct ggccagtgag cgtcatcggt gacccgtgcc tgcctcgtgg
     6301 tacaggctgc ggatcatagc ctgccgatgg cgcgcaacga gaaaggcccc gggattgccg
     6361 gggcctttct gattcgccag ggcaacggga cagtccccat gcggggacag tcccggggga
     6421 cagtccctag agcagcggca ccagcagcag tgccacgatg ttgatgatct tgat
//
LOCUS       DAOMFD010000112         6445 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4413_length_6445_cov_33.683255, whole genome shotgun sequence.
ACCESSION   DAOMFD010000112 DAOMFD010000000
VERSION     DAOMFD010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6445)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6445
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4413_length_6445_cov_33.683255"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..926)
                     /gene="prfA"
                     /locus_tag="PL152_07335"
     CDS             complement(<1..926)
                     /gene="prfA"
                     /locus_tag="PL152_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HQR49133.1"
                     /translation="MKDSIRRRLERTVERFEEVGALLAEPDAAATPGRFRDLSVEYSR
                     LEPVARRFREFTRLEAERSTAAEMAGGADVEMRELAEEELRSLDERIEAESGELAKLL
                     LPKDPRDESNIFLEVRAGTGGDEAAIFAGDLLRMYARYAERQGWKVEILSESPGEHGG
                     YREVISRVVGKGVYSRLKFESGTHRVQRVPATEAQGRIHTSACTVAILPELEEVDEVA
                     LNPADLRIDTYRASGAGGQHVNKTDSAIRITHLPTGIVVECQDERSQHKNRSRAMSLL
                     RARLLEAEQNKQVAQQAQERKLQVGSGDRSER"
     gene            complement(926..2176)
                     /gene="hemA"
                     /locus_tag="PL152_07340"
     CDS             complement(926..2176)
                     /gene="hemA"
                     /locus_tag="PL152_07340"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: protein motif:HMM:TIGR01035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008883 - glutamyl-tRNA reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HQR49134.1"
                     /translation="MSLVVVGINHRTAPVEVRERVVFEPTRLPEALRELRSLPAVSES
                     VIVSTCNRTEIYCMTEAGAGELGDWLQSYHQLGASIHHCLYRHEDTEAVNHVFSVASG
                     LDSLVLGEPQILGQLKDAYRAAQEVGTTGPALNRLFQAAFSVAKRVRTQTEIGANAVS
                     VAYAAVRMAKTVFASFDTRTALLVGAGETIALAARHLHADGLRRMIVANRSIERAREL
                     AAEFQGFAIGLDEIPSHLREADIVVASTASPNAIITHAMARDALRARKRRPMFMVDIA
                     VPRDIEPEVAELEDVYLFTIDDLQTVVNENMESRRQAAREATRMIASEVDRYALQLRT
                     REAAPTIRRLREHAEQARRHTLDQARHMLAAGRSAEDVIEFLSSTLTNRLIHAPSQRL
                     RDAAESGDDSVIETIADIYHLDRE"
     gene            2266..3948
                     /locus_tag="PL152_07345"
     CDS             2266..3948
                     /locus_tag="PL152_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR49135.1"
                     /translation="MRKALIHARRTLVAAVLLSPSAVVPAFGASSPSAAEPGPATLVA
                     QADAALARGDLPAAARAYRLAAEASDDEAVAEQASRVAFDNFQLSEALRSAERWLQIN
                     PSSEQAQRYAGVAALALHRLDVAESHFGTLVETAYVSPAAGFVALLPVIMDEATPADV
                     TELFRRLSASHPKVAEGHDALGNAALRSENYALAVRSAGEAVRLAPYWIPAKLLLARA
                     MIASGQEDEGLAMARDLAVAPDSDVATHMEYALMLASTGHDEEARAMLTPYATGNTVV
                     PAAVRTLGMLDLQSDDLKAANARFEELLSTGTQSYDALYFLGVVADRRNDEAGAVRYY
                     SRVIGGDQALAAQQRVALAKARSGGADAGLAHLDQFGRTQPSLGPQLVTARAGLLSTL
                     GDDKRALAVLDAGLEKYPDVLDLRLARVFAYERLGRGDASVRELRALLAERPGDPVVQ
                     NALGYTLADQDRELAEARALITESLAQTPDSPAVMDSMGWVLYRQKEYAEALGYLQRA
                     QERDDDAEICLHLGEVQWAMGDKDAARKTWREALERHPGARALEKRLASAGK"
     gene            3948..4538
                     /gene="lolB"
                     /locus_tag="PL152_07350"
     CDS             3948..4538
                     /gene="lolB"
                     /locus_tag="PL152_07350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein insertase outer membrane protein
                     LolB"
                     /protein_id="HQR49136.1"
                     /translation="MRRVAVLVVLATLGGCAATPVAPPPSVAAGASVPTDWTASGRLA
                     LAAHGQGGSGSFTWRQTESTTSLSLRGPLGAGAMQVTSAGGVLSVSDSEGRKLDDAET
                     RSLLRERLGADLPWTDLRYWMLGVPAPGSQSSVVDAGQAPLRVIEQVGWRIAYDAFDA
                     RAGVSLPTRFTATQADVRVRVIVDAWDLAPAGDAGP"
     gene            4535..5431
                     /gene="ispE"
                     /locus_tag="PL152_07355"
     CDS             4535..5431
                     /gene="ispE"
                     /locus_tag="PL152_07355"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HQR49137.1"
                     /translation="MSAPPGARGAEPLGRDEPWPAPGKINLFLHVVGRRADGYHLIQT
                     AYQFISLADELRFFRRQAGVIERLGDVPGVPADGDLVVRAARRLADDAGAAGLGVAIQ
                     VEKRLPMQAGVGGGSSDCATALVALNQLWRLGLSQDRLAEIGLELGADVPVFVRGQAS
                     WAEGVGERLTPMDFPETWYLLVRPDATVSTAEVFKAPELTRDSPVITIRDFLMTGGRN
                     DCEPVVRWRFPAVAEALEWLGRIAPARLTGTGSCVYAAMPGELEARAALGRLPARWTG
                     YVVRGQNRSPLAERCEREQGGH"
     gene            5454..5528
                     /locus_tag="PL152_07360"
     tRNA            5454..5528
                     /locus_tag="PL152_07360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5486..5488,aa:Gln,seq:ttg)
     gene            5581..>6445
                     /locus_tag="PL152_07365"
     CDS             5581..>6445
                     /locus_tag="PL152_07365"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HQR49138.1"
                     /translation="MERPTIAVFTGNACPQLAQDISRHLQVPLGRAHVGRFSDGEVTV
                     ELMENVRGRDVFVVQSTCPPANDHLMELLVMVDACRRASAGRITAVMPYFGYARQDRR
                     PRATRVAITAKVVANMLAGAGVHRVLTVDLHADQIQGFFDIPVDNVYSSPVLLGEVWK
                     QKYEDLVVVSPDVGGVVRARAIAKRLDDADLAIIDKRRPRANVSEVMNIIGEVEGKSC
                     VLVDDMVDTAGTLAQAAKALKDHGARRVAAYITHPVLSGPAVERIEASGLDELVVTDT
                     IPLRENARACRK"
BASE COUNT          932 a         2160 c         2349 g         1004 t
ORIGIN      
        1 atgcgctcgg agcggtcgcc gctgccgacc tgcagcttgc gctcctgggc ctgctgcgcg
       61 acctgcttgt tctgctcggc ctccagcagt cgtgcacgca gcagggacat cgcgcgcgac
      121 cggttcttgt gctgcgagcg ctcgtcctgg cactccacga cgatgccggt cgggaggtgc
      181 gtgatgcgga tcgcggagtc ggtcttgttg acgtgctggc cgccggcacc ggatgcgcgg
      241 tacgtgtcga tgcgcaggtc ggcgggattc aaggcgacct cgtcgacctc ctcgagttcc
      301 ggcagtatcg cgaccgtgca cgcggaggtg tggatgcgtc cctgcgcctc ggttgcgggc
      361 acgcgctgca cgcggtgcgt tccggactcg aacttgagcc tcgagtaaac gcccttgccg
      421 accacccggc tgatgacctc gcgatagccg ccgtgctcgc ccgggctctc gctcaggatc
      481 tcgaccttcc agccctgccg ctccgcataa cgcgcgtaca tgcgcaacag gtccccggcg
      541 aagatcgccg cctcgtcgcc gccggtgccg gcccgcacct cgaggaagat gttgctctcg
      601 tcgcgcggat ccttcggcag cagcagcttg gcgagctcgc cggattccgc ctcgatgcgc
      661 tcatcgaggc tgcgcagctc ctcctcggcc agctcgcgca tctcgacatc cgcgccgccc
      721 gccatctcgg cggccgtgct gcgctcggcc tcgaggcgcg tgaattcgcg gaatcgccgc
      781 gcgacgggct cgagccgcga gtactcgacc gacaggtcgc gaaagcggcc gggcgtcgcc
      841 gccgcatcgg gttcggccag gagcgcaccg acttcctcga accgctcgac cgtcctctcg
      901 agcctgcggc ggatggagtc cttcatcact cgcggtcgag gtggtagatg tcggcgatgg
      961 tctcgatgac ggagtcgtcg cccgactccg ccgcgtcgcg cagccgctga ctgggtgcgt
     1021 ggatcagccg gttggtgagc gtcgacgaca ggaattcgat cacgtcttcc gcgctgcgtc
     1081 cggcggcgag catgtgccgc gcctggtcca gcgtgtggcg ccgcgcctgc tcggcgtgct
     1141 cccggagcct gcggatggtg ggcgctgcct cgcgggtccg gagttgcagc gcgtagcgat
     1201 cgacctcgga ggcgatcatc cgggtggcct cgcgcgcggc ctggcgccgg ctctccatgt
     1261 tctcgttcac gacggtctgc aggtcgtcga tcgtgaagag atacacgtcc tcgagttccg
     1321 cgacctcggg ctcgatgtcg cgcggcaccg cgatgtccac catgaacatc ggccggcgct
     1381 tgcgcgcgcg gagcgcgtcc cgggccatcg cgtgcgtgat gatggcgttc gggctggcgg
     1441 tggacgcgac cacgatgtcg gcctcgcgca ggtgcgacgg gatctcgtcg agcccgatgg
     1501 cgaatccctg gaactcggcg gccagttcgc gcgcccgctc gatgctgcga ttggcgacga
     1561 tcatgcggcg caggccgtcg gcgtgcaggt gccgcgccgc gagcgcaatc gtttcacccg
     1621 ccccgaccag cagcgcggtg cgcgtgtcga agctcgcgaa gacggtcttc gccatgcgca
     1681 cggcggcgta cgcgaccgac accgcattcg cgccgatctc ggtctgcgtg cggacccgct
     1741 tggcgaccga gaaggccgcc tggaacaggc ggttcagcgc agggccggtc gtgccgacct
     1801 cctgggccgc ccgataagcg tccttgagct gcccgaggat ctgcggctcg ccgagcacca
     1861 gtgaatcgag gcccgacgcc accgagaaga cgtgattgac ggcctcggtg tcctcgtggc
     1921 ggtagaggca gtggtggatg ctcgccccga gctgatggta gctctgcagc cagtcgccca
     1981 gctcgccggc gcctgcctcg gtcatgcagt agatctcggt gcggttgcac gtggagacga
     2041 tgaccgactc gctgacggcc ggcaggctcc tcaattcacg caatgcctcg ggcaggcgcg
     2101 tcggctcgaa gaccacgcgc tcgcgcacct cgacgggcgc ggtgcggtga ttgatgccga
     2161 cgacgacgag ggacatccgg cttccggtcg cttaaaccgc gaaattctgg gttatgaccg
     2221 gtggcaacac aagcgcagcg gcgcttccgc tatagtgcgc ggaccatgcg gaaagccctc
     2281 attcacgccc ggcgcacgct cgtggcggcc gtcctgctgt cgccgagcgc cgtggtcccg
     2341 gccttcgggg cctccagtcc ttctgcggcc gagcccggac ccgcgacact ggtcgcccag
     2401 gccgatgccg cacttgcccg gggcgacctg cccgcggcgg cccgggccta ccggctcgcg
     2461 gcggaggcct ccgacgacga agcggtcgcc gagcaggcga gtcgcgtggc gttcgacaat
     2521 ttccagctga gcgaggcgct gcgttcggcg gagcgctggc tccagatcaa tccgtccagc
     2581 gagcaggccc agcgctacgc cggggtggcc gcgctcgcgc tgcatcgtct cgacgtggcc
     2641 gagtcgcact tcgggacact cgtcgagacg gcgtacgtca gcccggcggc ggggttcgtg
     2701 gcgttgctgc cggtcatcat ggacgaggcg acgccggccg acgtcaccga gctgttccgc
     2761 cggctgtcgg cgtcgcatcc gaaggtcgcc gaagggcacg atgcgctcgg caacgccgcg
     2821 ctgcgctcgg agaactatgc gctcgcggtc cgctccgccg gcgaggccgt gcgccttgca
     2881 ccgtactgga tcccggcgaa gctgctcctc gcccgcgcaa tgatcgcatc gggccaggag
     2941 gacgaaggcc tcgcgatggc gcgcgacctc gcggtggcac ccgactccga cgtggcgacg
     3001 cacatggaat acgcgctcat gctcgcgagt accgggcacg acgaggaggc gcgggcgatg
     3061 ctcacgccgt acgcaacggg gaacaccgtc gtgccggccg ccgtgcggac tctcggcatg
     3121 ctcgatctgc agagcgacga cctgaaggcg gcgaacgcgc gcttcgagga attgctgtcg
     3181 accgggacgc agtcttacga cgccttgtac ttcctgggcg tcgtcgcgga ccggcgcaac
     3241 gacgaggcgg gcgcggtccg ttattactcg cgcgtgatcg gtggcgacca ggcgctcgcc
     3301 gcgcagcagc gcgtggcgct cgccaaggcc cgctcgggcg gcgcggacgc aggtctcgcg
     3361 cacctcgacc agttcggacg cacgcaaccg tcgctcgggc cacaactcgt gacggcgcgt
     3421 gccggcttgc tgtcgacgct cggcgacgac aagcgcgcgc tcgccgtgct cgatgccggc
     3481 ctcgagaagt atccggacgt gctggacctg cgcctggcgc gcgtgtttgc ctatgagcgg
     3541 ctcgggcgag gggacgcgtc ggtgcgcgag ttgagggcgc tgctggcgga gcggcccggc
     3601 gacccggtcg tgcagaacgc gctcggctac acgctcgcgg accaggaccg ggagctcgcc
     3661 gaggcccgtg cgctgatcac cgagtcactg gcgcagacgc ccgacagccc ggcggtgatg
     3721 gacagcatgg gctgggtcct ctaccggcag aaggaatacg ccgaggcgct cggttacctg
     3781 cagcgcgccc aggaacgtga cgacgatgcc gagatctgcc tgcacctcgg cgaagtccag
     3841 tgggcgatgg gcgacaagga tgccgcgcgc aagacgtggc gggaggcgct cgagcgccat
     3901 cctggggcca gggcgctcga gaaacggctc gccagcgccg ggaagtgatg cgtcgcgtcg
     3961 ccgtcctggt cgtgctggca acgctcggtg gttgcgcggc caccccggtc gcacccccgc
     4021 cgtccgtcgc ggccggcgca tcggtgccga ccgattggac ggcaagcgga cggctcgcgc
     4081 tggccgccca cggccagggt ggcagtggct cgttcacgtg gcgacaaacc gagtcgacga
     4141 cgtccttgtc gttgcgcggg ccgctcggtg ccggcgcgat gcaggtgaca tccgccgggg
     4201 gcgtgctcag cgtctcggac agcgaaggcc ggaagctcga tgacgcggag acccggtcgc
     4261 tgctgcgcga gcggctcgga gccgacctgc catggaccga tcttcgctac tggatgctcg
     4321 gcgtgccggc gccgggcagc cagtcgagcg tcgtggatgc gggccaggcg ccgctgcgcg
     4381 tgatcgagca ggttggctgg cgcatcgcct acgatgcatt cgatgccagg gcgggggtgt
     4441 cgctcccgac gcgattcacg gcaacgcagg ccgacgtgcg ggtcagggtg atcgtcgacg
     4501 catgggacct tgctcccgcc ggggacgcgg gaccgtgagc gctccgcctg gagcgcgagg
     4561 cgccgagccg ctgggtcggg acgagccgtg gccggcgccc ggcaagatca acctgttcct
     4621 gcacgtcgtg gggcggcgag ccgacgggta ccacctgatt cagacggcat accagttcat
     4681 cagcctggcg gacgaactgc gcttcttccg acgccaggca ggggtcatag agcgactggg
     4741 cgatgtgccc ggcgtccctg ccgacgggga cctcgtggtt cgagcggcgc gccgactcgc
     4801 ggacgatgcg ggtgctgccg ggcttggggt ggcaatccag gtcgagaaga ggctgccgat
     4861 gcaggccggg gtggggggtg gaagttcgga ttgcgccacc gcattggtcg ccttgaacca
     4921 gttgtggcga ctcgggctgt cgcaggaccg actcgcggag atcgggctcg aactcggcgc
     4981 cgacgtcccg gtgttcgtgc gtggccaggc atcctgggcc gagggcgttg gggaacggct
     5041 gacaccaatg gattttcccg agacctggta cctgctggtc cgcccggacg ccacggtcag
     5101 caccgccgag gtgttcaaag cacctgaatt gacaagggat tccccggtaa tcacaattcg
     5161 cgatttcctg atgacgggtg ggcgcaacga ctgcgaaccg gtcgtcaggt ggcgctttcc
     5221 ggcagtggcg gaagcgctcg aatggctggg gcggatcgcg ccagcgcggc tgaccggcac
     5281 cggctcctgc gtatatgcgg cgatgccggg cgagctggag gcccgcgcgg cgctcgggcg
     5341 gttgccggcg cgctggacgg gttacgtcgt gcgcgggcag aaccgctcgc cgctggccga
     5401 gcgatgcgaa cgggagcagg gcgggcatta ggcccggccg ccgacgaatg cgctggggtg
     5461 tcgccaagcg gtaaggcagc gggttttgat cccgccattc ggaggttcga atccttccac
     5521 cccagccacc ggaaaaaggg gacattctcc tttttccagc ggaaaaagga gaatgtcccc
     5581 atggaacgcc cgacgatagc ggtgttcacc ggcaatgcgt gcccgcagct ggcgcaggac
     5641 atctcgcgcc acctgcaggt gccgctgggg cgcgcgcacg tcgggcggtt cagtgacggc
     5701 gaggtgacgg tcgagctgat ggagaacgtg cgcgggcgcg acgtgttcgt cgtgcagtcc
     5761 acgtgccctc cggccaacga ccacctcatg gagctgctgg tgatggtgga cgcctgccgg
     5821 cgcgcatcgg cggggcggat caccgccgtg atgccgtatt tcggctacgc gcgccaggat
     5881 cgccgaccgc gcgccacgcg cgtcgcgatc acggcgaagg tcgtggcgaa catgctcgcg
     5941 ggagccggtg tgcaccgggt gctcacggtc gacctgcacg ccgaccagat ccaggggttc
     6001 ttcgacatcc ccgtggacaa cgtgtattcc tcgccggtgc tgctcggcga ggtgtggaag
     6061 cagaagtacg aggacctcgt ggtcgtgtcg cccgacgtgg gcggcgtggt gcgcgcgagg
     6121 gcgatcgcca agcgcctcga cgacgcggac ctcgcgatca tcgacaagcg ccggccgcgg
     6181 gccaacgtgt ccgaggtgat gaacatcatc ggcgaggtcg agggcaagag ctgcgtcctc
     6241 gtcgacgaca tggtcgatac cgccggcacg ctggcccagg cggcgaaggc attgaaggac
     6301 cacggcgcga ggcgcgtcgc ggcctacatc acccacccgg tgctgtcggg cccggcggtg
     6361 gagcggatcg aggcctcggg gctcgacgaa ctggtggtga cggacacgat cccgctgcgc
     6421 gagaacgcgc gcgcgtgccg caaga
//
LOCUS       DAOMFD010000113         6383 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4472_length_6383_cov_35.738148, whole genome shotgun sequence.
ACCESSION   DAOMFD010000113 DAOMFD010000000
VERSION     DAOMFD010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6383)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6383
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4472_length_6383_cov_35.738148"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /gene="aceE"
                     /locus_tag="PL152_07370"
     CDS             complement(<1..590)
                     /gene="aceE"
                     /locus_tag="PL152_07370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647860.1"
                     /note="E1 component; part of pyruvate dehydrogenase; forms
                     a complex with DlaT and LpdC; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HQR49139.1"
                     /translation="MFEASKIEDTDPAETREWLESIDSVLRTQGAERAHYLLERVIDY
                     TRRSGAHLPFTPNTAYVNTISAGQEKEYPGDRALERRIKAYIRWNAMAMVVQANRQSS
                     EYGGHIASYASAATLYEVGFNHFWRAPGDAHPGDMVFIQGHSSPGIYARAFLEGRLTE
                     DQLRRFREEVGGGGLSSYPHPWLMPDFWQFPTVSMGL"
     gene            686..2212
                     /locus_tag="PL152_07375"
     CDS             686..2212
                     /locus_tag="PL152_07375"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HQR49140.1"
                     /translation="MTTLLPPDTPVEIRGRAGSPTEASVARADGVILLVDAAHPERAL
                     ARLPHAQLWLALHRKARPRPGRAPMQLVTRLPNARHTLAVVSFAKSGESAFDRLEACR
                     SLVTTVLQSGVGRLQCHVAVPRTDEAAAWREAMVSAALAVAAPMPQRKSKAPPPCDLR
                     RVEVYGAKTGEFDDSVAVAAGNHLARWLAALPPNELNCVTYRRALRDLAVREGWSFRF
                     YDERALARLGAGAFLAVARANAHRGAGIVRLRYRAAGRGPRPRIGLVGKGICFDTGGI
                     NLKAQKGMFMMHGDMQGSAVAVGTLLACSRSRARVDIDCWLALTENEIGPRAYRPQDI
                     VRAANGVTIQVVHSDAEGRMALADTLVLASRERPRLLLDFATLTGACVNALTERYSGA
                     FTNRLDLHQPVQQAGRDSGERVWCFPMDGDFDEEIDSSVADVMQCTMDSKGDHILAAR
                     FLAHFVEPNVPWVHVDLSSSERKGGLAHVPTEFTGFGVRFATRLLGDAALLARLPGTA
                     "
     gene            2209..3246
                     /gene="pyrC"
                     /locus_tag="PL152_07380"
     CDS             2209..3246
                     /gene="pyrC"
                     /locus_tag="PL152_07380"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HQR49141.1"
                     /translation="MTTLTLRRPDDWHLHLRDGAHMAAVLPMSAAQFARALVMPNLRP
                     PMTTTQALGEYRSRILSALPRGARFEPWMTLYLTDRTPPEEIVRAKRSGFVLGAKLYP
                     AGATTHSDAGVTSIEHTWATLEAMAEHGLVLQVHGEVTDPDVDVFDREHAFIERVLAR
                     VLERVPGLRVVFEHITTAAAVEFVRGAPAGVSATITPQHLLMNRNAIFEGGLRPHHYC
                     LPVLKRERDREALLAAATGDDPRFFLGTDSAPHARHTKEAACGCAGIFSAHAAVELYA
                     EAFDQAGRIDRLEAFASERGADFYGLPRNAERITLTRESWTVPQRYPFGDDTLVPLRA
                     GSEISWRLVQA"
     gene            3243..3929
                     /gene="rnt"
                     /locus_tag="PL152_07385"
     CDS             3243..3929
                     /gene="rnt"
                     /locus_tag="PL152_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016896 - exoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HQR49142.1"
                     /translation="MSGDLEPPGIASRFRGFLPIALDVETGGFNAGTDALLEIAAVVI
                     EMTPFGELRPGPNFRYHVQPFQGSRIDPASLAVNGIDPHHPLRPALPEKEALTQLFRE
                     IRKAVRDTGCTRAILVGHNAAFDLGFVNAAVARTGIKRNPFHPFSCFDTATLGGVALG
                     QTVLSRAVTAAGLDWHPDSAHSAIYDAERTAALFCTICNRFRFMYEEALERAPELAVT
                     DAGEAPPADE"
     gene            complement(3964..4287)
                     /gene="grxD"
                     /locus_tag="PL152_07390"
     CDS             complement(3964..4287)
                     /gene="grxD"
                     /locus_tag="PL152_07390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Grx4 family monothiol glutaredoxin"
                     /protein_id="HQR49143.1"
                     /translation="MDVIERIQGQLKSNPVVLYMKGTPDFPQCGFSASAVRTLEACGA
                     KFASFNIFEDPELRDALKRYSNWPTYPQLYVNGELVGGSDILTEMYQKGELQSLLAEA
                     GATTR"
     gene            complement(4292..4507)
                     /locus_tag="PL152_07395"
     CDS             complement(4292..4507)
                     /locus_tag="PL152_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003157722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HQR49144.1"
                     /translation="MIEKVAPGSPCVNVCVLDAAGCCTGCGRTLDEIAGWGAMSAGER
                     WAVIARLEAARAARARPTMAANDAAAR"
     gene            4643..5245
                     /locus_tag="PL152_07400"
     CDS             4643..5245
                     /locus_tag="PL152_07400"
                     /inference="COORDINATES: protein motif:HMM:NF017945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate hydroxylase PuuD"
                     /protein_id="HQR49145.1"
                     /translation="MNPLTSVRGSIITGFVVALVITFAIAPTGFMELGLARWFHILSG
                     VMWIGLLWYFNVVQVPALAVAAADKGGPGGAGISKYVAPRALLWFRWAAVATWLTGAW
                     YLGRSGNFLGAFTLGMSGEALNYYQLVIGIGAWLGTIMLFNVWVLIWPNQQKVLGLVP
                     ASDEEKAKARKVALYASRTNFILSIPMLMCMASATHGLPF"
     gene            5268..6140
                     /gene="nadC"
                     /locus_tag="PL152_07405"
     CDS             5268..6140
                     /gene="nadC"
                     /locus_tag="PL152_07405"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HQR49146.1"
                     /translation="MTGAVATAPPPDLHDQVARALAEDVGTGDLTAALVPPGRTGRAT
                     VVTREPAVLCGQPWFEEVFRQLDASVRVHWDAAEGSVVATDQRLCRLEGPARSLLTGE
                     RTALNFLQTLSGTATVARRYSDLLAGLPCRVLDTRKTLPGLRRAQKYAVRCGGGSNHR
                     MGLYDGILVKENHIMAAGSIAAAVATARKQGAAVPVEVEVESLDELKQALDAGADMAL
                     LDEFSLDDLRAAVALNRSHPRGPIKLEASGSVTFDTLRSIAETGVDFVSVGSLTKHVR
                     AIDLSMRFEFTAEK"
     gene            complement(6192..>6383)
                     /locus_tag="PL152_07410"
     CDS             complement(6192..>6383)
                     /locus_tag="PL152_07410"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR49147.1"
                     /translation="HDPIDREMVRSINEIGHLTGKLTIAEFAENTEIINMLRSLGVDY
                     AQGYGIASPQRIMKVAANG"
BASE COUNT          963 a         2249 c         2148 g         1023 t
ORIGIN      
        1 aggcccatcg acaccgtcgg gaactgccag aagtccggca tcagccacgg atgcggatac
       61 gatgacagcc cgccaccacc cacctcctcg cggaagcggc gcaactggtc ctcggtgagc
      121 cgtccctcga ggaacgcgcg cgcgtagatg cccggcgacg agtgtccctg gatgaagacc
      181 atgtcgcccg ggtgcgcatc gccgggcgcg cgccagaagt ggttgaaccc gacttcgtac
      241 agcgtggccg ccgaggcgta gctcgcgatg tgaccgccgt actcggaact ctgccggttg
      301 gcctgcacca ccatcgccat ggcgttccag cggatgtagg ccttgatgcg ccgctcgagc
      361 gcgcggtcgc cggggtattc cttctcctgg cccgctgaga tcgtgttgac gtacgccgtg
      421 ttcggcgtga acggcaggtg ggctccggag cggcgcgtgt agtcgatgac gcgctcgagg
      481 aggtagtgcg cccgctcggc gccctgcgtc cgcaagacgg agtcgatcga ctccagccac
      541 tcgcgcgtct cggccggatc ggtgtcttcg atcttcgagg cttcgaacat ggtgcgcgcc
      601 cgcgataatg atttgcgcgg cacgccctgc tagcatgccg acgcgcaatg atcagcgcac
      661 gtccccgcag ggtcaaggca ggttcgtgac cacgttgcta ccaccggaca caccggtcga
      721 gattcgcggg cgcgcgggct cgcccacgga ggccagtgtc gcgcgcgcag acggcgtgat
      781 cctgctcgtc gacgcagctc acccggagcg ggcgctggcc cggctgccgc acgcccagct
      841 gtggcttgcg ctgcaccgca aggcgcggcc acgccccggc cgcgcgccca tgcagctcgt
      901 cacgcgcctg cccaatgcgc gtcacacgct cgcggtggtg tcgttcgcga agtccggcga
      961 gagtgcgttc gaccgcctcg aggcttgccg cagcttggtg accacggtgc tgcagtcggg
     1021 cgtgggccgg ctgcaatgcc acgtcgcggt cccgcgcacc gacgaggcgg ccgcgtggcg
     1081 cgaggcaatg gtcagcgcgg cgctggcggt cgcggccccg atgccgcagc gaaagtccaa
     1141 ggcaccgccc ccgtgcgacc tgcgccgcgt cgaggtctac ggcgcgaaga ccggcgagtt
     1201 cgacgacagc gtagcggtcg cggccggcaa tcacctcgcg cgctggctgg ccgcactccc
     1261 gccgaacgag ctgaactgcg tgacctaccg tcgggcgctg cgcgatctcg cggtgcgcga
     1321 gggctggtcg ttccgcttct acgacgagcg cgctctcgcg cgcctcggcg cgggcgcgtt
     1381 cctcgcggtc gcgagggcca acgcacatcg cggcgccggg atcgtgcgac tgcgctaccg
     1441 tgcggcaggc cgcggcccgc gccctcgcat cggcctcgtc ggcaagggca tctgcttcga
     1501 caccggcggc atcaacctca aggcccagaa gggcatgttc atgatgcacg gcgacatgca
     1561 gggcagcgcc gtcgccgtcg gcacgctgct cgcgtgctcg cgaagccggg cccgagtcga
     1621 catcgactgc tggctcgcgc tcacggagaa cgagatcggc ccgcgcgcgt atcgcccgca
     1681 ggacatcgtg cgggccgcga atggggtcac catccaggtg gtgcacagcg atgccgaagg
     1741 tcgcatggcg ctggccgaca cgctggtcct ggcctcgcgg gagcggcctc gcctgctcct
     1801 ggacttcgcg acgctcacgg gggcctgcgt gaacgcgctc accgagcgct acagcggcgc
     1861 attcaccaat cgcctcgacc tgcaccagcc ggtccagcaa gcgggtcggg acagcggcga
     1921 gcgcgtctgg tgcttcccga tggacggcga tttcgacgag gagatcgaca gcagcgtcgc
     1981 cgacgtcatg cagtgcacca tggactcgaa gggcgaccac atcctcgccg cgcggttcct
     2041 ggcgcacttc gtcgagccga acgtgccctg ggtgcacgtg gacctgtcgt cgtccgagcg
     2101 caagggcggg ctcgcgcacg taccgaccga gttcaccggc ttcggcgtgc gcttcgccac
     2161 gcggctgctg ggcgacgcgg cgctgctcgc gcggctcccc ggaacggcat gacgacgctc
     2221 acgctgcgcc gcccggacga ctggcacctg cacctgcgag acggcgcaca catggccgcc
     2281 gtcctgccga tgtcggccgc gcaattcgcg cgcgcgctgg tcatgccgaa cctgaggccg
     2341 ccgatgacga cgacacaggc cctcggcgag taccgctcgc gaatactttc ggcattgccg
     2401 cgcggtgcgc gcttcgagcc gtggatgacc ctgtacctca cggaccgcac gccgccggag
     2461 gaaatcgtcc gcgcgaagcg cagtggattc gtgctgggcg ccaagctcta cccggccggt
     2521 gcgaccaccc actccgacgc gggcgtgacg tccatcgagc acacctgggc gacactcgaa
     2581 gcgatggccg aacacgggct cgtactgcag gtgcacggcg aggtcaccga cccggacgtg
     2641 gacgtgttcg accgcgagca cgcgttcatc gagcgcgtac tggcccgcgt gctcgagcgc
     2701 gtgcccggcc tgcgcgtcgt gttcgagcac atcacgaccg cggccgccgt cgagttcgtg
     2761 cgcggagccc cggccggtgt ctcggcgacg atcacgccgc agcacctgct catgaaccgc
     2821 aacgcgatct tcgagggcgg cctgcgcccg caccattact gcctgccggt gctcaagcgc
     2881 gagcgcgatc gcgaggcgct gctggcggcc gccacgggcg acgacccgcg gtttttcctc
     2941 ggcaccgaca gcgcgccgca cgcccggcac accaaggagg cggcctgcgg ctgtgcaggc
     3001 atcttctcgg cgcacgccgc ggtcgagctg tatgcggagg ccttcgacca ggccggacgc
     3061 atcgaccggc tcgaggcctt cgcgtccgag cgtggcgccg atttctacgg cctgccgcgc
     3121 aatgcggaac gcatcacgct cacgcgcgag tcgtggaccg ttccgcagcg ctatcctttc
     3181 ggcgacgaca cgctggtccc gctgcgcgcc ggctcggaaa tcagctggcg gctggtgcag
     3241 gcgtgagcgg cgacctcgag ccgcccggaa tcgcgagccg gttccgcgga ttcctgccga
     3301 tcgcgctcga cgtcgagacg ggcgggttca atgccggcac ggacgcgctg ctcgagatcg
     3361 ccgcggtcgt gatcgagatg acgccgttcg gcgaactgag gccggggccc aatttccggt
     3421 accacgtgca gccgttccaa ggttcgcgca tcgacccggc ctcgctcgcg gtgaacggca
     3481 tcgacccgca ccatccactg cggccggcgc tgcccgagaa ggaagcgctc acgcagctgt
     3541 tccgcgagat ccgcaaggcc gtgcgcgaca cggggtgcac tcgcgcgatc ctggtcgggc
     3601 acaacgccgc gttcgacctg ggattcgtga atgccgcggt ggcccgcacg ggaatcaagc
     3661 gcaacccgtt ccatcccttc tcgtgcttcg acacggcgac gctcggcggc gtcgcgctgg
     3721 gacagacggt gttgtcgcgc gcggtcacgg ccgcgggcct cgactggcac ccggattcgg
     3781 cgcattcggc gatctacgat gcggaacgca ccgcggcgct gttctgcacc atctgcaatc
     3841 gcttccgctt catgtacgaa gaggcgctcg agcgcgcgcc cgaactcgcc gtgacggacg
     3901 ccggcgaggc cccgccggcc gacgaataaa tatccaggtc ggtgcctggc accgattgcg
     3961 gaattatctg gtcgtggcgc cggcctcggc gagcagcgac tgcagctcgc ccttctggta
     4021 catctcggtc aggatgtcgg agccgccgac cagctcgccg ttcacgtaca gctgcggata
     4081 cgtcggccag ttcgaatagc gcttcagggc gtcgcgaagc tccgggtcct cgaagatgtt
     4141 gaaggacgcg aatttcgcgc cgcaagcctc gagcgtgcgc accgccgacg cggagaaccc
     4201 gcactgcggg aagtccggcg tgcccttcat gtacagcacg accgggttcg acttgagctg
     4261 gccctggatt ctctcgatga cgtccacctg ttcacctcgc agccgcgtcg ttcgcggcca
     4321 tggtcgggcg cgccctggcg gcacgcgcag cctcgagccg tgcgatcacg gcccaccgtt
     4381 cccctgcgct cattgctccc cagccggcga tctcgtcgag cgttcggccg catcccgtgc
     4441 aacagccggc ggcgtccagc acgcagacgt tgacgcaggg agaccccggg gcgaccttct
     4501 cgatcaatcg caagctctcg tgcagcgcag aaggcgttga attatgcgga tgcgtcgcct
     4561 attcttcaaa cctctcgcgg cgggccgcaa acgaacctaa ccgcctgtcg ccattgagat
     4621 ttccagtagc ggaggacatt ccatgaaccc ccttacctcg gttcgcggct cgatcatcac
     4681 cggattcgtg gtcgcgctgg tcatcacttt cgcgatcgcc cccaccggat tcatggaact
     4741 cggcctcgcc cgctggttcc acatcctgtc gggcgtcatg tggatcggcc tgctctggta
     4801 cttcaacgtc gtccaggtcc cggcgctcgc ggtcgcggcc gccgacaagg gtggtcccgg
     4861 cggcgcgggc atctcgaaat acgtcgctcc gagggcgttg ctgtggttcc gctgggcggc
     4921 ggtcgccacc tggctcaccg gtgcgtggta cctcgggcgc tccggcaact tcctcggcgc
     4981 attcacgctc ggcatgagcg gcgaagcgct gaactactac caactcgtca tcggcatcgg
     5041 cgcgtggctc ggcacgatca tgctgttcaa cgtgtgggtc ctcatctggc cgaaccagca
     5101 gaaggtgctg gggctggtgc cggcgtccga cgaggagaag gccaaggcgc gcaaggtggc
     5161 gttgtatgca tcgcgcacca atttcatcct gtcgatcccg atgctgatgt gcatggcgtc
     5221 ggcgacgcac gggctgccgt tctgaggcgt cgggacgtgc cggggcgatg actggcgcag
     5281 tcgcgaccgc gccgcccccc gacctgcacg accaggtcgc acgtgcgctc gccgaggacg
     5341 tgggcaccgg cgacctgacc gccgccctcg tcccgcctgg ccgcaccgga cgcgccacgg
     5401 tcgtcacgcg cgaaccggcg gtgctctgtg ggcagccttg gttcgaagag gttttccgcc
     5461 agctcgacgc ttcggtgcgc gtccactggg acgcggccga aggttcggtc gtggccaccg
     5521 accagcgcct ctgcagactc gaggggccgg ctcgctcgct cctcacaggc gagcgcactg
     5581 ccctcaattt cctgcagacg ctctccggca cggcgacggt ggcacgccgt tacagcgacc
     5641 tgctggccgg cctgccctgc cgcgtcctcg acacgcgcaa gaccctcccg ggcctcaggc
     5701 gcgcgcagaa gtacgcggtg cgctgcggtg gcggcagcaa ccaccgcatg ggtctctacg
     5761 acgggatcct cgtcaaggag aaccacatca tggcggccgg atcgatcgcg gcagcggtgg
     5821 cgaccgcgcg caagcaaggc gctgccgtgc cggtcgaagt ggaagtcgag tcactggatg
     5881 aactcaagca ggcgctcgat gcgggtgccg acatggcgct gctcgacgaa ttcagcctcg
     5941 atgacctccg cgccgccgtc gcactgaacc gctcgcaccc tcggggcccg atcaagctcg
     6001 aggcgtcggg cagcgtcacg ttcgacacac tgcggtccat cgcggagacc ggcgtcgact
     6061 tcgtctccgt cggttcactc accaagcacg tgcgggcgat cgacctgtcg atgcggttcg
     6121 aattcaccgc tgagaaatag gggacattcc cctgtttcgc gaagcggaaa caggggaatg
     6181 tcccctattt ctcagccgtt cgcggcgacc ttcatgatcc gctgcggcga ggcgatgcca
     6241 tagccctgcg cgtaatcgac gccgaggctg cgcagcatgt tgatgatctc ggtgttctcg
     6301 gcgaactccg cgatggtgag cttgcccgtc aggtgcccga tctcgttgat cgagcgcacc
     6361 atctcgcggt cgatcgggtc gtg
//
LOCUS       DAOMFD010000114         6294 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4578_length_6294_cov_31.701875, whole genome shotgun sequence.
ACCESSION   DAOMFD010000114 DAOMFD010000000
VERSION     DAOMFD010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4578_length_6294_cov_31.701875"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(202..582)
                     /locus_tag="PL152_07415"
     CDS             complement(202..582)
                     /locus_tag="PL152_07415"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HQR49148.1"
                     /translation="MAKKLIIVMANTDTRNGEELGAPIFQATVAAAMEYEVEVICTAT
                     AGRLMKKGVAETLHVKPGSPKTVYDFIKDAADAGAKFYCCSPNLDLFDMSKDDLIPEC
                     AGIIGGAHLIEEVMENEDAKVLTY"
     gene            complement(704..1396)
                     /locus_tag="PL152_07420"
     CDS             complement(704..1396)
                     /locus_tag="PL152_07420"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQR49149.1"
                     /translation="MKTLNVLQHTSAEYLGLIEDHFEGRRIRFRYSRPFATGGRVPKA
                     GQLLDGLVMLGGGPWGSAGGRNVPTLIEELTLARAALDRGLPILGIGLGAQLLAIVAG
                     GAVEDAPLAFEVGEARRVDPGALDGYLPERYPLVVYGRDRPILPAAARVLSVDAAGRP
                     ALFQVGPRALGFTGHPGVKPAIVEDLIMEFDESPPDPGPRLVELRTLQRSIEDALVPI
                     MTGVVKVLGLMD"
     gene            complement(1393..2469)
                     /locus_tag="PL152_07425"
     CDS             complement(1393..2469)
                     /locus_tag="PL152_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoate A/B protein ligase family
                     protein"
                     /protein_id="HQR49150.1"
                     /translation="MAGAFRVVDTGVREGRYNIALDQAMLELHQSGQIGDTFRFMRFP
                     PTALIGRHQALRQEVDVDHCRSHGVGIVRRITGGGAIYLDEGQLGWGLIFGRRALRPG
                     SLSELARDICLAVAAGLRRVGVDARYRPRNDIEVDGRKVSGTGGFFDGDTLIYQGTVL
                     VDLNPAAMVEALRVPRAKLEKRQLDSAAQRVVTLRELLGPSTPTLPAIQKALLEAFAE
                     RFGLQWTEEPLSPAEDSLAWQRYREEIGTDQFVAEVDDPARDADFLAGSHTGPGGTVT
                     SYVRLEGPARKRIGVALITGDFFVAPPRTILDLEAALRGAYVDDAPGIIERFFVEHPI
                     SALTVKPEDFRASLEAALAGGARG"
     gene            complement(2474..3733)
                     /locus_tag="PL152_07430"
     CDS             complement(2474..3733)
                     /locus_tag="PL152_07430"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HQR49151.1"
                     /translation="MSCINQGTESTTAAAADGRSFNSYASMEPRAPLPTPVEARNGRR
                     VKDTAVPPLGVYLPNNNVLTPDMRSPDYVQMSTAAAITLGVMAGRMHRCECTRCLNLL
                     LTYPEGCRANCAYCGLARHREADRDYADRNFIRVDWPAVPMAEIVERVARDGERSPFH
                     RMCISMITHPRSDADTVSVLKAWTSRIDPATIPVSILSNPTTMQRDDVARLRDLGADI
                     FTVALDAATPGIFDRTRGKGVQSPHKWSKYWEILHHARDVFGPQKFGAHIIVGMGETE
                     RELLTLVQQLVDLGGHSHLFSFFPEAGSLMDHLPATPRDQWRRVQLARYLIDYAGVRV
                     EQMGFDAEGRVTGYGLPTAEVDRIVEEGVAFRTSGCPGKFRDDVSACDRPYGDSPPSN
                     IASYPFQPGKQDLRRIRRQLKIPVVQG"
     gene            complement(3763..4800)
                     /locus_tag="PL152_07435"
     CDS             complement(3763..4800)
                     /locus_tag="PL152_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49152.1"
                     /translation="MSWLESLGRIEDLEDVAFDAELVRGFEATAATTAARPIRFSTPT
                     FKDYASTELKGCSKNSFPSFSITAGACGLNCDHCRAEILKPMIPATSPEMLDQKVRRL
                     VATQNLQGFLLSGGSNRRNEIRYERYYPVVERLKRDFPAMKIAIHTALTDERGAKAME
                     SAGVDTAMMDVIGADETIRDVYHLDRPVADFEATLAALCSTSMEVSPHIVIGLHYGRI
                     LGEPDALDIVSRHPVTALVLVVVMPFYARPDTYAVPDPDDVGRIFGEARRRLADRQVL
                     LGCARPPGLHKRVVDAYAVMAGLDGIAFPADGAVAVANAIGRPFHQEHACCSIKVGAA
                     PRVTSASTCAA"
     gene            complement(4787..5935)
                     /locus_tag="PL152_07440"
     CDS             complement(4787..5935)
                     /locus_tag="PL152_07440"
                     /inference="COORDINATES: protein motif:HMM:NF013046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HQR49153.1"
                     /translation="MMQRSAGAVRRVAPPLFVGSHFCRLPSFGRNHPLAIPRVATVID
                     LCTSLGWLDGPSFRTAPAASIEQLATFHDPAYVEALRDADAQGHVEPAVRERHNFGNF
                     ENPLFGGVFERAATAVGGSILAAELALEGRAVFHPAGGTHHGRRQRASGFCYFNDPVF
                     AILTLLGNGCDRVLYVDLDAHHCDGVQDAFQDEDRVRLVSIHEAGRWPHTGLADDGSS
                     NAINLPVPRDCNDTEFRSLMDGIVLPAAAAFAPDSVVVTCGTDALAGDPLSRMALSNL
                     ALWDAVLALTSLGTPAVVLGGGGYNPWTLARCWTGLWGRLLGQDIPQPLPPQARLCLS
                     GLHCDLVEDEAIRPEWLTTLADTPREGPVRDEIRQLVDRHAGRMHELA"
     gene            6060..>6294
                     /locus_tag="PL152_07445"
     CDS             6060..>6294
                     /locus_tag="PL152_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011737635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HQR49154.1"
                     /translation="MSDSSGAKRMSIIVTKGTLDWAYPPFILSTTAAAMGLDVTLFFT
                     FYGLPLLLKDLDLKMSPLGNPAMKMPMMGGHMGM"
BASE COUNT          948 a         2137 c         2223 g          986 t
ORIGIN      
        1 gtggccgtgc agatgccgca gttgaggcac ccgttcagct cgtggtcgaa gcgcaggtcg
       61 gactggatgt ccgcgaagat cgcctcgagc ttctcacgcg ggaccggcgg cgcgtcggag
      121 accgggtcgc cgatgtattc ctggacgcgc gtggcggcgt gttccatctc ttccccccgg
      181 gccggcgtgc cggctcaagc ctcagtacgt caggaccttc gcgtcctcgt tctccatgac
      241 ttcctcgatc aggtgcgcgc cgccgatgat gccggcgcac tccgggatca ggtcgtcctt
      301 gctcatgtcg aagaggtcga ggttcggcga gcagcagtag aacttcgcgc cggcgtcggc
      361 cgcgtccttg atgaagtcgt acacggtctt cggcgaaccc ggcttcacgt gcagcgtctc
      421 ggccacgccc ttcttcatca gtcgcccggc cgtcgcggtg catatcacct cgacctcgta
      481 ctccatcgcc gccgcgacgg tcgcctggaa gatcggcgca ccgagttcct cgccgttgcg
      541 ggtgtcggtg ttcgccatga cgatgatcag cttcttggcc atgtccgttc gctccgtcgg
      601 cggatgctgc ggcgcaacgc gccggggcgg ccccggcgcc gccaaagtat tagcgcaccc
      661 taatatagtt gcagccgggt cgcaacagga aaaacgcgcc cgctcagtcc atgaggccga
      721 gcaccttcac cacgcccgtc atgatcggca cgagcgcgtc ctcgatgctg cgctgcaacg
      781 tgcgcagctc gacgaggcgc gggcccggat cgggcggaga ctcgtcgaat tccatgatca
      841 ggtcctcgac gatcgcgggt ttcacgccgg gatgtccggt gaaaccgagc gcccgcgggc
      901 cgacctggaa cagggcgggg cgcccggccg cgtcgaccga caggacacgc gccgccgctg
      961 gcaggatcgg ccggtcgcga ccgtacacga cgagcggata gcgttccggc aggtagccgt
     1021 cgagcgcccc cgggtcgacg cgccgggcct cgccgacctc gaaggccagc ggggcatcct
     1081 ccacggcgcc accggcgacg atggcgagca actgcgcgcc gagcccgatc ccgaggatgg
     1141 gcaggccgcg atcgagtgcg gcgcgggcca gggtgagttc ctcgatcagg gtcgggacat
     1201 tccggccgcc ggcgctgccc cacggcccgc cgccgagcat cacgaggccg tcgaggagct
     1261 ggccagcctt tggcacccgg cctcccgttg caaatggccg cgagtagcgg aagcggatac
     1321 gccgtccctc gaaatggtcc tcgatgagcc cgaggtattc ggccgacgtg tgctgcagga
     1381 cgttgagcgt cttcacccgc gcgccccgcc ggccagcgcg gcttcgaggg acgcgcggaa
     1441 atcctcgggc ttcaccgtca gcgcgctgat cgggtgctcg acgaagaacc gctcgatgat
     1501 cccgggcgcg tcgtcgacgt aggcaccgcg cagcgccgcc tcgaggtcga ggatcgtgcg
     1561 cggcggcgcc acgaagaagt cgccggtgat gagcgcgacg ccgatgcgct tgcgggccgg
     1621 accctcgagg cgcacgtagc tcgtcactgt gccgcccggg cccgtgtggg agcccgccag
     1681 gaaatccgcg tcgcgcgccg ggtcgtccac ctcggccacg aattgatcgg tgccgatctc
     1741 ctcgcggtag cgctgccacg cgaggctgtc ctccgccggc gacagcggct cttccgtcca
     1801 ttgcagcccg aaccgctcgg cgaatgcctc gagcagcgcc ttctggatgg ccggcagggt
     1861 cggggtcgac gggccgagca gctcgcgcag ggtcacgacg cgttgcgcgg cggaatcgag
     1921 ctggcgtttc tcgagcttgg cgcgcggcac gcgcagcgcc tcgaccatcg ccgcgggatt
     1981 caggtcgacc agcaccgtgc cctggtagat gagcgtgtcg ccgtcgaaga agccgcccgt
     2041 gccgctcacc ttgcggccgt ccacctcgat gtcgttgcga ggacggtagc gcgcgtcgac
     2101 cccgacccgc cgcagcccgg ccgccacggc gaggcagatg tcccgcgcga gctcggacag
     2161 cgaacccggc cgcagcgccc tgcgcccgaa gatgagcccc cagccgagct ggccctcgtc
     2221 gagatagatc gccccgccgc cggtgatgcg gcgcacgatc ccgacgccgt ggctgcggca
     2281 atggtccacg tcgacttcct ggcgcagcgc ctggtgccgc ccgatcagcg cggtgggcgg
     2341 gaagcgcatg aagcggaacg tgtcgccgat ctgcccggac tggtgcagct cgagcatggc
     2401 ctggtcgagc gcgatgttgt agcggccctc ccgcacgccg gtatcgacga cgcggaacgc
     2461 gccggccacg gcactagccc tggacgacgg ggatcttgag ctgcctgcgg atgcgccgca
     2521 ggtcctgctt gccgggctgg aacgggtagc tcgcgatgtt gctgggcggc gagtcgccgt
     2581 aaggtcgatc gcacgcggac acgtcgtcgc ggaacttgcc cgggcagccc gaggtccgga
     2641 atgcgacgcc ctcctcgacg atgcggtcga cctctgctgt cggcaggccg tagccggtga
     2701 cgcgtccttc ggcgtcgaag cccatctgct cgacgcgcac gcccgcgtag tcgatcaggt
     2761 agcgcgcgag ctgcacgcgg cgccactggt cccgcggcgt ggccggcagg tggtccatga
     2821 gcgagccagc ctcgggaaaa aagctgaaga gatgactgtg cccgccgagg tccacgagct
     2881 gctgcacgag cgtgagcagt tcccgctcgg tctctcccat ccccacgatg atgtgcgcgc
     2941 cgaatttctg cggcccgaac acatcgcgcg cgtgatgcag gatttcccag tacttcgacc
     3001 acttgtgcgg cgactgcacg cccttgccgc gcgtgcggtc gaagatcccg ggcgtggcgg
     3061 cgtcgagcgc cacggtgaag atgtcggccc caagatcccg gagcctggca acgtcatctc
     3121 gctgcatggt ggtcgggttc gagaggatcg acaccgggat ggtcgcgggg tcgatgcgcg
     3181 aggtccacgc cttcagcacc gagacggtgt cggcgtccga ccgtggatgc gtgatcatcg
     3241 agatgcacat gcggtggaag gggctccgct ccccgtcgcg tgccacgcgc tcgacgatct
     3301 cggccatcgg caccgcgggc cagtccacgc ggatgaagtt gcggtcggcg tagtcgcgat
     3361 cggcctcgcg atggcgggcg agcccgcaat acgcgcaatt cgcgcggcag ccctcggggt
     3421 aggtgagcag caggttcagg cagcgcgtgc actcgcagcg gtgcatcctg cccgccatca
     3481 cgccgagcgt gatcgccgcg gccgtggaca tctgcacgta gtccggcgag cgcatgtcgg
     3541 gcgtcaggac gttattgttc ggcaggtaca cgccgagcgg cgggaccgcc gtgtccttca
     3601 cgcggcggcc gttgcgcgcc tcgacgggcg tgggcagcgg cgcgcgcggc tccatgctcg
     3661 cgtaggaatt gaacgaccgg ccgtccgctg cggctgcggt ggtggactcg gtgccctggt
     3721 tgatgcagct catcgacgaa gtctcctggc cctgcggccc tgtcaggccg cgcaggtgct
     3781 ggctgaggtc acgcgcggcg cggccccgac cttgatcgag caacaggcgt gttcctggtg
     3841 gaaggggcgg ccgatcgcgt tcgcgaccgc gaccgcgccg tcggcgggga aggcgatgcc
     3901 gtcgagccct gccatcacgg cataggcgtc gacgactcgc ttgtgcaggc cgggcggccg
     3961 cgcgcagccg agcaggacct ggcggtccgc aagacgccgc cgtgcctcgc cgaaaatgcg
     4021 tccgacgtca tcaggatcgg gcacggcgta ggtgtcgggc ctggcgtaga agggcatgac
     4081 gacgacgagc acgagcgccg tgaccggatg gcggctcacg atgtcgagtg catccggctc
     4141 gccgaggatg cggccgtagt gcaggccgat cacgatgtgc gggctgacct ccatcgacgt
     4201 ggagcacagg gccgcgagcg tggcctcgaa atcggccacc gggcggtcga ggtggtacac
     4261 gtcgcggatg gtctcgtccg cgccgatgac gtccatcatc gccgtgtcga cgccggcgga
     4321 ttccatggcc ttcgcgccgc gctcgtcggt gagcgcggtg tggatcgcga tcttcatggc
     4381 cgggaaatcc cgcttcaggc gctccaccac ggggtaatag cgctcgtagc ggatctcgtt
     4441 gcgccggttc gagccacccg acagcaggaa gccctgcagg ttctgtgtcg ccaccagccg
     4501 gcgcaccttc tggtcgagca tctcggggga ggtcgccggg atcatcggct tcaggatctc
     4561 ggcgcggcag tggtcgcaat tcaatccgca ggcgccggcg gtgatcgaga aggacgggaa
     4621 gctgttcttc gagcagccct tgagctcggt gctcgcgtag tccttgaacg tcggcgtcga
     4681 gaaccggatg ggtcgcgcag ccgtcgtcgc cgcagtggct tcgaagccgc gcacgagctc
     4741 cgcgtcgaat gcgacgtcct cgaggtcctc gatgcggccg agtgattcaa gccagctcat
     4801 gcatcctccc cgcgtgcctg tcgaccagtt ggcggatctc gtcgcgtacc gggccctcgc
     4861 ggggtgtgtc ggcgagggtc gtgagccatt cgggccggat ggcctcgtcc tcgacgaggt
     4921 cgcagtgcag gcccgacaag cataaccgcg cctgcggcgg caggggttgc gggatgtcct
     4981 gcccgagcag gcggccccac aggccggtcc agcagcgagc gagtgtccac gggttgtacc
     5041 cacccccgcc cagcacgacc gccggcgtcc cgaggctcgt gagcgcaagc acggcgtccc
     5101 acagcgcgag gttgctcaat gccatgcgcg acagcgggtc gcccgcgagc gcgtccgttc
     5161 cgcaggtcac gaccaccgaa tccggcgcaa aggccgccgc cgctggcaat acgatcccgt
     5221 ccatcaggct gcggaattcc gtgtcgttgc agtcgcgcgg tacgggaaga ttgatcgcgt
     5281 tgctgctgcc gtcgtcggcc aagccggtgt ggggccagcg tccggcctcg tggatcgaga
     5341 ccagccggac ccggtcctcg tcctggaacg cgtcctgcac cccgtcgcag tgatgcgcgt
     5401 cgagatcgac gtaaagcacg cggtcgcagc cgttcccgag cagggtcagg atcgcgaaga
     5461 ccgggtcgtt gaagtagcag aacccgctgg cgcgctggcg gcggccgtgg tgggtccctc
     5521 cggccgggtg gaacaccgcg cggccctcga gcgcgagttc ggcggccagg atcgagccgc
     5581 cgacggcggt ggcggcccgc tcgaacacgc cgccgaacag cgggttctcg aagttgccga
     5641 agttgtgccg ctcgcgcact gcgggctcga cgtgtccctg cgcatcggca tcgcgcagag
     5701 cctcgacgta ggccgggtcg tggaatgtcg cgagctgctc gatgcttgcg gccggcgcgg
     5761 tccggaacga cggcccgtcg agccagccga gcgaggtgca caggtcgatg acggtggcga
     5821 cgcgagggat ggcgagcggg tgattgcggc cgaacgacgg cagcctgcag aagtggctgc
     5881 cgacgaagag cgggggcgcg acgcgacgca ccgcacctgc gctgcgctgc atcatgacct
     5941 gcccttggcc gccggggatc tgcaccaccc gagcgtaata aattcgactt tgctaataaa
     6001 gacgtctctg ttaagttgcc gtcgctcggc aggcagagac caaacgtggg gggcacggta
     6061 tgtccgattc gtccggcgcc aagcgcatgt ccatcatcgt caccaagggc acgctcgact
     6121 gggcgtaccc gccgttcatc ctgtcgacca cggcggctgc catgggcctc gacgtgacgc
     6181 tgttcttcac cttctacggc ctgccgctgc tgctcaagga cctcgacctc aagatgtccc
     6241 cgctcggcaa cccggcgatg aagatgccga tgatgggcgg gcacatgggc atgc
//
LOCUS       DAOMFD010000115         6254 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4621_length_6254_cov_31.113083, whole genome shotgun sequence.
ACCESSION   DAOMFD010000115 DAOMFD010000000
VERSION     DAOMFD010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6254)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6254
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_6254_cov_31.113083"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="PL152_07450"
     CDS             complement(<1..814)
                     /locus_tag="PL152_07450"
                     /inference="COORDINATES: protein motif:HMM:NF022853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD family protein"
                     /protein_id="HQR49155.1"
                     /translation="MHIAWVQLALVALGCLGAGRTIAQDTTSSGFDVRRMLGGESQEG
                     QLLPADKAFQVTVFSDRADRITLHWDIAKGYYLYRDKVKVAATSGNAQLGNPSIPGGK
                     VKHDDYFGDQVVFFEQMDAEVPVAAAAGTREVTVEVRYQGCAEAGLCYPPIRKTFPVQ
                     LGAMAGIATSTAKSSGAPVQRSEQDLLADRIRSGSLLAVLATFFGAGLLLAFTPCVLP
                     MVPILSGIIVGAGRDRPVSRGRAFALSLAYVLGMAFTYTVAGAAFAAAGRQAQ"
     gene            1171..1473
                     /gene="groES"
                     /locus_tag="PL152_07455"
     CDS             1171..1473
                     /gene="groES"
                     /locus_tag="PL152_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003966899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HQR49156.1"
                     /translation="MADKLKIRPLHDRVIIQRLDAETKSPGGIVIPDTAAEKPVRGKV
                     VAVGKGKILEDGTVRPLDVKVGDHILFGKYSGTEVKLDGEEYLVMREEDVMAVLEK"
     gene            1516..3159
                     /gene="groL"
                     /locus_tag="PL152_07460"
     CDS             1516..3159
                     /gene="groL"
                     /locus_tag="PL152_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019456581.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HQR49157.1"
                     /translation="MAAKDVRFSDDARARMLRGVNILANAVKVTLGPKGRNAVLEKSF
                     GAPTVTKDGVSVAKEIELKDKFENMGAQMVKEVASNTSDEAGDGTTTATVLAQAIVRE
                     GLKAVAAGANPMDIKRGIDKAVETATEELKKLSKPCKDQKAIAQVGTISANSDESIGK
                     TIADAMEKVGKEGVITVEEGSGLQNELDVVEGMQFDRGYLSPYFINSQANQTAELENP
                     YILLYDKKISNVREMLPLLEGIAKAGRPLLVVAEDVEGEALATLVVNTIRGIVKVAAV
                     KAPGFGDRRKAMLQDIAVLTGGTVIAEEVGLSLEKASLADMGQAKKVVVEKENTTIID
                     GHGKPAEIKARIAQIKQQIEEATSDYDKEKLQERMAKLSGGVAVIKVGAATEIEMKEK
                     KARVEDALHATRAAVEEGVVPGGGVALIRAQKALEKLEGANEDQAIGIKILARSIEEP
                     LRQIVANAGEDAAVVLADVKKGKGTYGYNAATGVYGDMLDMGILDPTKVTRLALQNAS
                     SVAGLLLTTEVMVAEAPKDEEHKHGGGMPDMGGMGDMGM"
     gene            3483..4523
                     /locus_tag="PL152_07465"
     CDS             3483..4523
                     /locus_tag="PL152_07465"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HQR49158.1"
                     /translation="MADVFISYARSDKARVAPLVAAIVSRGWTVWWDPEITPGQEFDD
                     MIDAEIAAAKAVMVVWTPTSVTSRWVRGEAREAADSNKLVPVRFDNARLPMDVRAIHT
                     TDLDGWKDDPQSPQAREFLQALGAMIARAPTRAVNAAEAAEDAPDKPEPRFTICVLPF
                     TNMSGDPEQEYFSDGITEDVITDLSKVSALGIVSRNSAFMYKGKPVDVPKVAAELKVS
                     HVLEGSVRKAGGRVRISAQLVDAQTNNHIWAERYDREASDIFALQDEISEAIVKALRL
                     RLLPKEKKAIERRGTDNPDAHNLYLMARQTYVNSHDIDGRAAEAIVRLCDRAIEIDPN
                     YAQAWTLKAIGF"
     gene            4563..5264
                     /locus_tag="PL152_07470"
     CDS             4563..5264
                     /locus_tag="PL152_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49159.1"
                     /translation="MAAAERALALDPDLAEAHAVKAQLLLGDGAAEQAAEEVAIALRL
                     NPESYEVNRSAGRLSYQLHRYQDAVRFYEKSAGLMETDLNSASILISTYTALGDDASM
                     KRAAQLTLKRAEAVLAHDQNNSMVAGYSAYALAALGEGGRAKARMERALLIDPDNFNM
                     RYNFACALCVYLRDKQAALDMLEPLFATITDTFLPYAKADPDLELLRGESRYHDMVTA
                     AEERLAKAAQVNSSK"
     gene            complement(5350..5811)
                     /locus_tag="PL152_07475"
     CDS             complement(5350..5811)
                     /locus_tag="PL152_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49160.1"
                     /translation="MFLKKHCGTLGQCNLAISGTRKKFAQIRLGDRTLTDVATYSDEL
                     ADAEYRNRDMCLYLFRVLTTNYLIGWKRRDTGDKLLLPGPIVRNWFLQIAVVYAFVYL
                     FAAFFGALLLLTVLGVKGSTSLGGYAAVAGVLLAWYYAVTMLLAYREAKAD"
BASE COUNT         1215 a         2025 c         2066 g          948 t
ORIGIN      
        1 cctgcgcctg gcgcccggca gccgcgaagg ccgcgccggc gaccgtgtag gtgaatgcca
       61 tgccaagcac gtaggcgagc gacagggcaa atgcccggcc gcgcgacacg ggccggtcgc
      121 ggcccgcgcc cacgatgatg cccgacagga tcggcaccat cggcaggacg cagggcgtga
      181 acgcgagcag caatcccgcg ccgaagaacg tcgcgagcac cgcgagcagg ctgccgctgc
      241 gaatgcggtc ggccagcaag tcctgctccg agcgctgcac gggcgctccg ctgctcttcg
      301 ccgtcgaagt ggcgatccct gccattgccc cgagctgcac cgggaacgtc ttgcggattg
      361 gcgggtagca gagcccggct tcggcgcatc cctggtagcg cacttcgacc gtgacttcgc
      421 gcgtgccggc tgccgccgcg accggcacct ccgcgtccat ctgctcgaag aacacgacct
      481 ggtcgccgaa atagtcgtcg tgcttcacct tgccgccggg aatggacgga ttaccgagct
      541 gtgcattgcc actcgtggca gcgaccttga ccttgtcgcg atagaggtaa tagcccttgg
      601 cgatgtccca gtgcagcgtg atgcgatcgg cgcggtcgct gaacacagtg acttgaaagg
      661 ccttgtcggc cggcaggagc tggccttcct gcgattcgcc gccgagcatg cgccggacgt
      721 cgaagccaga cgacgtggtg tcctgggcaa tcgtccggcc cgcccccagg cagcccagcg
      781 caaccagtgc gagttggacc caggcgatat gcatcttgcg aatcatcggt ccccggctcg
      841 ggtcgccggg acgaccctta gaaaggttcg catcatgccc atccgcgtcg cggaccgcga
      901 cctcgggtgt ccgaaaagtg catcgccgca caggaatatc tttcccgttc atagggttgt
      961 tcgagtcgac gcccggtcgg ggcagcaccg gcggccggat caggcccggc caccccatgc
     1021 accgtcatgg acccacccgg atgtgacccg gttccacccc agggcttgaa atcggccttt
     1081 cgccgcctat cattagcagc cgcttggggt gagtgctaac accatcccgc gatttcccct
     1141 cccagctgaa caaataatgg agtttcagca atggctgaca agttgaagat tcgcccgctc
     1201 cacgaccgcg tcatcatcca gcggctcgac gccgagacca agtctcccgg cggcatcgtg
     1261 atcccggaca ccgcggccga gaagccggtc cgcggcaagg tcgtcgccgt gggcaagggc
     1321 aagatcctcg aggacggcac ggtccgcccg ctcgacgtca aggtcggcga ccacatcctc
     1381 ttcggcaagt acagcggcac cgaagtgaag ctcgatggcg aggaatacct cgtcatgcgc
     1441 gaagaagacg tgatggcggt gctcgagaag taagcgcccg cccgcaacca gcaacgaatt
     1501 ccggagaaca ctgcaatggc agcgaaagac gtacgtttca gcgatgacgc ccgcgcgcgg
     1561 atgctccgcg gcgtcaacat cctcgcgaat gcggtcaagg tgaccctggg tcccaagggc
     1621 cgcaacgcgg tgctcgagaa gagcttcggc gcccccaccg tcaccaagga cggcgtgtcg
     1681 gtcgcgaagg agatcgagct caaggacaag ttcgagaaca tgggcgcgca gatggtgaag
     1741 gaagtcgctt ccaacacctc cgacgaggcc ggtgacggca cgacgaccgc cacggtgctc
     1801 gcccaggcga tcgtgcgcga gggcctgaag gccgtcgcgg cgggcgccaa cccgatggac
     1861 atcaagcggg gcatcgacaa ggccgtcgag acggccaccg aggagctgaa gaagctctcc
     1921 aagccctgca aggaccagaa ggcgatcgcg caggtcggca cgatctcggc gaacagcgac
     1981 gagtcgatcg gcaagaccat cgccgacgcg atggagaagg tcggcaagga aggcgtgatc
     2041 acggtcgagg agggctcggg cctgcagaac gagctcgacg tcgtcgaggg catgcagttc
     2101 gaccgcggct acctctcgcc gtacttcatc aacagccagg ccaaccagac ggccgagctc
     2161 gagaacccgt acatcctgct gtacgacaag aagatctcga acgtccgcga gatgctcccg
     2221 ctgctcgagg gcatcgccaa ggccggccgt ccgctgctcg tcgtggccga ggacgtcgag
     2281 ggtgaggcgc tcgccacgct ggtcgtcaac accatccgcg gcatcgtcaa ggtcgcggcc
     2341 gtgaaggccc cgggcttcgg cgaccgccgc aaggccatgc tgcaggacat cgccgtcctc
     2401 accggcggca ccgtgatcgc cgaggaagtg ggcctctcgc tcgagaaggc ctcgctcgcc
     2461 gacatgggcc aggccaagaa ggtcgtggtc gagaaggaga acaccacgat catcgacggc
     2521 cacggcaagc cggccgagat caaggcgcgc atcgcgcaga tcaagcagca gatcgaggaa
     2581 gccacctccg actacgacaa ggagaagctg caggagcgca tggcgaagct ctccggcggc
     2641 gtcgccgtga tcaaggtcgg tgccgcgacc gagatcgaga tgaaggagaa gaaggcccgc
     2701 gtcgaggacg cgctgcacgc cacccgtgcg gccgtcgagg aaggcgtggt gccgggcggc
     2761 ggcgtggcgc tgatccgcgc gcagaaggcg ctcgagaagc tcgagggcgc gaacgaggac
     2821 caggcgatcg gcatcaagat cctggcccgc tcgatcgagg agccgctgcg ccagatcgtc
     2881 gcgaacgcgg gcgaggacgc cgcggtcgtg ctggccgacg tcaagaaggg caagggcacg
     2941 tacggctaca acgccgcgac gggcgtctat ggcgacatgc tcgacatggg catcctggac
     3001 ccgaccaagg tcacgcgtct cgcgctgcag aacgcctcct cggtggcggg cctgctgctc
     3061 acgacggaag tgatggtcgc cgaggctccg aaggacgagg agcacaagca cggcggcggc
     3121 atgccggaca tgggcggcat gggcgacatg ggcatgtaag gtcgcgagac tttacggctc
     3181 gatgaacgag gcccgggagg cgactcccgg gcctctgtcg ttgtgggccg acggcaaggt
     3241 ctcggatact gcagccgaca ggtgaaccgc tgccgagcgt ccgggcaacg gtcgcggcag
     3301 gctctggctg cgcatcgagt ccaccgcgcc aacacgcctg cgcggatcgg agatctgaaa
     3361 ttcctgtccg cgcacggagc atggcctcgg gtcaaaaaag tgccctgacc cgctttggtc
     3421 cgtgggttat gcttggtcgc tccaccgcgc cgaccgtttg aattctcagg gatttcaact
     3481 gcatggccga cgtcttcatt tcctacgctc gcagcgacaa ggcccgcgtc gcaccgctgg
     3541 tagcggccat cgtgtcgaga ggctggaccg tgtggtggga ccctgagatc acgccggggc
     3601 aggaattcga cgacatgatc gatgccgaga tcgcggcggc caaggccgtg atggtcgttt
     3661 ggacgccgac ctcggtgacc tctcgatggg tgcgcggcga ggcacgcgag gcagccgaca
     3721 gcaacaagct tgtgccggtg cgattcgata atgcgcgcct gccgatggac gtgcgggcca
     3781 ttcacaccac ggatctcgac ggctggaaag acgatccaca gagcccgcag gctcgcgaat
     3841 tcctgcaggc gctgggcgcg atgatcgcgc gggctccgac cagggcagtg aacgccgcgg
     3901 aagcggcgga ggacgcgccg gacaagcccg agccgcgctt taccatctgc gtgctgccgt
     3961 tcaccaacat gagcggtgac ccggagcagg agtacttcag cgacggcatc accgaggacg
     4021 tgatcaccga tctcagcaag gtctccgcgc tcggcatcgt ctcgcgcaac agcgccttca
     4081 tgtacaaggg caagcctgtc gacgtgccga aggtcgcggc ggaactcaag gtcagccacg
     4141 tgctggaggg cagtgttcgg aaggcgggcg gccgcgtgcg catcagcgcg caactcgtcg
     4201 atgcccagac caacaatcac atctgggcgg aacgctacga tcgggaagca agcgacatct
     4261 tcgcgctcca ggatgagatt tccgaagcca tcgtcaaggc cttgcggttg cgcctgctgc
     4321 cgaaggagaa aaaggcgatc gagcgtcgcg ggacggacaa cccggacgcg cacaacctgt
     4381 acctgatggc gcgccagacc tatgtcaaca gccacgacat cgacggtcgg gcggccgagg
     4441 ccatcgtccg actgtgtgat cgcgcgatcg agatcgaccc gaactacgct caggcttgga
     4501 cactgaaggc gatcggcttt tgaaaactgc gcgaggcgag cggtggcaag acggacgaag
     4561 gcatggcggc ggcggagcgc gccctggcgc tcgatcccga tctggctgag gcgcatgccg
     4621 tcaaggcgca acttctgctg ggggatgggg cggccgagca agccgccgag gaagtcgcca
     4681 tcgcgctgcg tctgaatccc gagtcctatg aggtcaaccg gtcggcgggc cgattgagtt
     4741 accagttgca tcgctaccag gacgcggtcc gcttctatga gaagtccgcc gggctgatgg
     4801 agacggacct caactccgcc tccatactga tcagcaccta tacggctctt ggcgatgacg
     4861 cctcgatgaa acgcgctgcg caactgaccc tgaaacgcgc cgaggccgtt ctggcgcacg
     4921 accagaacaa ctcgatggtg gctggctaca gcgcgtacgc gctggcggca ctcggcgaag
     4981 gcgggcgggc gaaagcgcgc atggagcgtg cgctgctgat cgatccggac aacttcaaca
     5041 tgcgctacaa ctttgcctgt gccttgtgcg tgtatctcag ggacaagcag gccgcgctgg
     5101 acatgctcga gccgctgttc gccacgatca ccgatacgtt cctgccgtac gccaaggcag
     5161 acccggatct ggagttgctg cgcggcgaat cgcgatatca cgatatggtc accgccgccg
     5221 aggaacggct ggctaaggcg gcacaggtca actcatcgaa atgagccggc ccggcggccc
     5281 acggctcggg ctcctcgaat ccagccgccg cccaacagaa ctagcgccgg acctcgctcg
     5341 caacgaaatt cagtccgcct tcgcttccct ataggccaac agcatcgtca cggcgtagta
     5401 ccaggcgagc agtacgccag cgacggctgc gtacccgccg agcgaagtgc tgcccttcac
     5461 tccgagtacc gtgaggagga gcagcgctcc gaagaaagcc gcgaacaggt acacgaacgc
     5521 gtagaccacc gctatctgca gaaaccaatt ccgtacgatg ggcccaggca gaagcagctt
     5581 gtcgcccgtg tcgcgccgtt tccaaccgat caggtagttc gtcgttagca cgcggaaaag
     5641 atagaggcac atgtcgcgat tccggtactc cgcgtcggcc aattcgtcgc tgtaggtcgc
     5701 gacgtcggtc aacgtgcgat cccccaggcg aatctgcgca aacttcttgc gcgttccgga
     5761 gatggccaga ttgcactggc ccaacgtgcc gcaatgcttc ttgaggaaca tgaccccccc
     5821 ctcggatgcc gcggtacgcc cgtcggcgtt gccgggcgac gcgaagcccc acaccgacca
     5881 ccgaggttac gcctgcttcg ccaggttttg cacgcgtcga cttgttggcg gcggcgcgca
     5941 gcgctgggaa taccttgagg tgggtctgga cgcgaggcag acggtgtaga gcgccgtgcg
     6001 gcgcgtgatt gtgtggcagg tgccgccaag gccgtagccg agcgccagcg tggcgccgta
     6061 gaacgcaatt ccagcgcgat ttgatgaact ggcgcgcgac cgatcccgta ccatccgcgg
     6121 atcgccgtca cctcggacgc agtccatgcc cctcgcctgg ctctcgctcg cgatcgcctg
     6181 caccgtcggt tatcacctgg tgctgaagct cacgccgccg ggcgtgaacc cgctgctgtc
     6241 gctgctggtg acct
//
LOCUS       DAOMFD010000116         6133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4818_length_6133_cov_34.915104, whole genome shotgun sequence.
ACCESSION   DAOMFD010000116 DAOMFD010000000
VERSION     DAOMFD010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4818_length_6133_cov_34.915104"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(15..794)
                     /gene="folE2"
                     /locus_tag="PL152_07480"
     CDS             complement(15..794)
                     /gene="folE2"
                     /locus_tag="PL152_07480"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012536877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003933 - GTP cyclohydrolase activity
                     [Evidence IEA];
                     GO_process: GO:0035998 - 7,8-dihydroneopterin
                     3'-triphosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase FolE2"
                     /protein_id="HQR49161.1"
                     /translation="MDDVQGRADSRQIPINKVGVKDIYHPVRIRDRSGDEQHTIANFN
                     MYVALPHNFKGTHMSRFVEVLHGHEREISVDSFRQMLKEVTRRLDATSGHVEMSFPYF
                     MMKTAPVSGVPSLMNYQASLVGEIHDGHEEMWIHVVVPVTSLCPCSKKISEYGAHNQR
                     SHITIRARVREHVWIEELVDIAEQEASSQLYGILKRPDEKFVTERAYDNPKFVEDIVR
                     DVAVRLNREPRVLAYVVEAENFESIHNHSAYALIEKDKEAG"
     gene            complement(797..2707)
                     /gene="dxs"
                     /locus_tag="PL152_07485"
     CDS             complement(797..2707)
                     /gene="dxs"
                     /locus_tag="PL152_07485"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HQR49162.1"
                     /translation="MSLADRYPLLAAIESPADLRRLPESQLVPLTQELREFLVHTVSQ
                     MGGHFAAGLGTVELTVALHYVYDTPHDRLVWDVGHQAYPHKVLTGRRDRLQTIKLKDG
                     LAPFPSRAESEYDTFGVGHASTSIGAALGMALASKHTGETRRIAAVIGDGAISGGQAF
                     EAMNHAGPLDPDMLVVLNDNKMSISENVGALAKYLARVLSGRTYTALRESSKKILDRM
                     PRMKRFARRSEEHMKGMVLPGTLFEELGFNYSGPIDGHDVLELVAALRQLRERHGLQF
                     LHVVTQKGKGYAPAEADPIKWHGPGPFDPTSGTIYKDKATGPSYSQVFGQWLCDMAER
                     DPRVVGITPAMREGSGLVEFSRRFPDRYYDVAIAEQHAVTLAAGMACEGARPVVAIYS
                     TFLQRGYDQFIHDVALQNLAVTFAIDRGGLVGGDGATHQGAYDLSFLRCIPNVVVMAP
                     ADENECRQMLYTAVMHGGPAAVRYPRGQGPGVPIVGEMTALPIGRAEVRREGRSGLLL
                     LAFGTMVAPCEPIAARLDATLVNMRFVKPLDAELVRRLAASHECIVTIEENAVAGGAG
                     SAVSECLAATGVERIVHHVGIPDRFIEHGSREDCLAAAGLDAAGVAQQIERLWPARNG
                     ILAAVGGRPLAV"
     gene            complement(2748..3638)
                     /locus_tag="PL152_07490"
     CDS             complement(2748..3638)
                     /locus_tag="PL152_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HQR49163.1"
                     /translation="MGSLSSRLDEWRSRVEQELERWLPPDSAVPQRLHAAQRYGVLGP
                     GKRIRPALVYATAETLGVALSTVDAAACAVELIHAYSLVHDDLPAMDDDDLRRGRPTC
                     HKAFDEATAILAGDSLQVLAFQVLASHAGVPDDRIRVRMIAILAEASGTAGMAGGQAL
                     DLAAEGRTLGLAEIEQVHALKTGALIRASILMAAQCAGDLGLARLARLGEFGSLVGLA
                     FQVQDDILDIEGDAALIGKPVGSDEARAMPTYPAVAGIAPAKARVRELHEAAGAILRR
                     EGWAGSPLAAVADWLVTRRH"
     gene            complement(3650..3889)
                     /gene="xseB"
                     /locus_tag="PL152_07495"
     CDS             complement(3650..3889)
                     /gene="xseB"
                     /locus_tag="PL152_07495"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF002137.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HQR49164.1"
                     /translation="MTSKKPKSPDFEQALADLEAVVERLEHGELPLEDALKQFERGIE
                     LARSCQASLKAAEQRVEILLKKTPDAEPEPFEEEP"
     gene            4107..4523
                     /locus_tag="PL152_07500"
     CDS             4107..4523
                     /locus_tag="PL152_07500"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HQR49165.1"
                     /translation="MKNLKGTKTEKNLKDAFAGESQANRRYLYFAQKADVEGFNDVAA
                     VFRSTAEGETGHAHGHLEYLEQVGDPATGLPIGSTSNNLKASIAGETHEYTDMYPGMA
                     KSARDEGFDEIADWFETLAKAERSHANRFQKALDAL"
     gene            4642..5994
                     /locus_tag="PL152_07505"
     CDS             4642..5994
                     /locus_tag="PL152_07505"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heterodisulfide reductase-related iron-sulfur
                     binding cluster"
                     /protein_id="HQR49166.1"
                     /translation="MAEGTRTPAAGEGSLAAPTRHPIAWREPDFYDEESLFKELHRVY
                     DLCHGCRRCFSLCNAFPLLFDAIDASPTGELDGVDRKVYWEVADHCYLCDMCYMTKCP
                     YVPPHPWNIDFPHLMLRAKAVRFRKQGARLRDRILTSTDAVGTLAGIPVVAQAVNLVN
                     RTKFGRTVLDKTMGVHPGAPVPDYHSNSGRKRVARGDHAGLEVKPTTETKGRVALFAT
                     CYCNRNEPDIDADLAAVFRHNGIPVSVIARERCCGMPKLELGDLEAIEQAKNVNVPAM
                     AALVDSGHDLVAPIPSCVLMFKQELPLLYPDDPLVAKVKAAMFDPFEYLMLRHKAGLL
                     RTDFVRQLGKVSYHVPCHLRVQNLGLKTREVLSLIPGTEVDAIERCSGHNGTYGVKRE
                     YRDISMKIGKPVINRVQAASADFYASDCPMAGHQIESGLEKAAGPTHPLTLLRMAYGL
                     "
BASE COUNT          960 a         2088 c         2116 g          969 t
ORIGIN      
        1 gtgcgcccgc gcactcagcc ggcttccttg tccttctcga tcagcgcgta cgccgagtgg
       61 ttgtggatgg actcgaagtt ctcggcctcg accacgtacg cgagcacgcg cggctcgcgg
      121 ttgagccgca ccgcgacgtc gcgcacgatg tcctcgacga acttcgggtt gtcgtaggcg
      181 cgctccgtca cgaacttctc gtcggggcgc ttcaggatcc cgtagagctg gctcgacgcc
      241 tcctgctcgg cgatgtcgac cagttcctcg atccagacgt gctcgcggac cctggcccgg
      301 atcgtgatgt gcgagcgctg gttgtgcgcg ccgtactcgg agatcttctt cgagcagggg
      361 cagaggctcg tgaccggcac caccacgtgg atccacatct cctcgtgccc gtcgtggatc
      421 tcgccgacca ggctggcctg gtagttcatc aggctcggca cgcccgagac cggcgccgtc
      481 ttcatcatga agtaggggaa gctcatctcc acgtgtcccg acgtcgcgtc gagccggcgg
      541 gtgacttcct tcagcatctg gcggaacgag tccaccgaga tctcccgctc gtggccgtgc
      601 agcacctcga cgaagcgcga catgtgcgtg cccttgaagt tgtgcggcag ggccacgtac
      661 atgttgaagt tcgcgatcgt gtgctgctcg tcgccggagc ggtccctgat ccgcaccggg
      721 tggtagatgt ccttgacccc gaccttgttg atgggaatct gccgcgagtc cgcgcggcct
      781 tgcacgtcgt ccatcatcag accgccagtg gccggccgcc cacggcggcc aggatcccgt
      841 tgcgagcggg ccagagccgc tcgatctgct gtgcgacgcc cgccgcgtcg aggcccgcgg
      901 ccgcgaggca gtcctcgcgc gagccgtgct cgatgaaccg gtcggggatg ccgacgtggt
      961 gcacgatgcg ctcgaccccg gtcgcggcga ggcattcgct caccgcgctg ccggcgccgc
     1021 ccgcgacggc gttctcctcg atggtcacga tgcactcgtg cgacgccgcc agccggcgca
     1081 ccagctcggc gtcgagcggc ttgacgaagc gcatgttgac gagcgtcgcg tcgaggcgcg
     1141 cggcgatcgg ctcgcaaggc gcgaccatgg tgccgaatgc gagcaacagc aggcccgagc
     1201 gtccctcgcg ccggacctcg gcgcgtccga tgggcagcgc ggtcatctcg cccacgatcg
     1261 gcacgcccgg gccctggccg cgcggatagc gcacggccgc cggcccgccg tgcatgacgg
     1321 cggtatagag catctgccgg cactcgttct cgtcggccgg cgccatgacg accacgttcg
     1381 ggatgcagcg caggaagctc aggtcgtacg cgccctggtg cgtggcgccg tcgccgccca
     1441 cgagtccgcc gcggtcgatc gcgaacgtga cggcgaggtt ctgcagcgcg acgtcgtgga
     1501 tgaactggtc gtagccgcgc tgcaggaacg tcgagtagat ggccaccacc gggcgcgcgc
     1561 cttcgcaggc catgccggcg gcgagcgtca cggcgtgttg ctcggcgatc gcgacgtcgt
     1621 agtagcggtc ggggaaacgc ctggagaact cgacgaggcc ggaaccttcg cgcatcgcgg
     1681 gcgtgatgcc gacgacgcgc gggtcgcgct ccgccatgtc gcacagccac tggccgaaca
     1741 cctgcgagta ggacggcccg gtggccttgt ccttgtagat cgtcccgctg gtcgggtcga
     1801 acgggcccgg cccgtgccac ttgatgggat cggcttccgc gggcgcatag cccttgccct
     1861 tctgcgtcac cacgtgcagg aactgcagcc cgtgccgctc gcgcagctgg cggagcgccg
     1921 cgaccagctc gagcacgtcg tggccgtcga tcggaccgct gtagttgaag ccgagctcct
     1981 cgaagagcgt gcccgggagc accatgccct tcatgtgctc ctcggagcgg cgcgcgaagc
     2041 gcttcatgcg cggcatgcgg tcgaggatct tcttgctgct ttcgcgcagc gcggtgtagg
     2101 tgcgccccga caggacgcgc gcgaggtact tggcgagtgc acccacgttc tccgaaatgg
     2161 acatcttgtt gtcgttcagc acgaccagca tgtccgggtc gagggggccc gcgtgattca
     2221 tcgcctcgaa cgcctggccg ccgctgatgg cgccgtcgcc gatcaccgcg gcgatgcggc
     2281 gcgtctcgcc ggtgtgcttc gacgcgagcg ccatgcccag cgccgcgccg atcgaggtgc
     2341 tcgcgtgccc gacgccgaac gtgtcgtact cactctcggc gcgactcggg aacggggcga
     2401 ggccgtcctt gagcttgatc gtctgcagcc ggtcgcgccg gcccgtcagc accttgtggg
     2461 ggtaggcctg gtggcccacg tcccagacca gccggtcgtg gggcgtgtcg tacacgtaat
     2521 ggagcgcgac cgtgagctcg accgtcccca ggccggccgc gaagtggccg cccatctggc
     2581 tcaccgtgtg gaccaggaat tcccgcagct cctgcgtgag gggcacgagc tgcgactccg
     2641 ggaggcgtcg caggtccgcc ggcgactcga tcgccgcgag caggggatat cggtcggcca
     2701 gggacatagg gcggcaagtt taccgcaccg cggcaaggcg gaccggttca gtggcggcgg
     2761 gtcaccagcc agtccgcgac ggccgccagg gggctccccg cccagccctc gcgccggagg
     2821 attgccccgg ccgcctcgtg cagttcgcgc acgcgtgcct tggccggcgc gatgccggcg
     2881 acggccggat aggtcggcat cgcgcgcgcc tcgtcgctgc ccaccggctt gccgatcagg
     2941 gccgcgtcgc cctcgatgtc caggatgtcg tcctgcacct ggaaggcgag gccgacgagg
     3001 ctgccgaatt cacccagtct ggcaagccgg gcgagcccga ggtcccctgc gcactgggcc
     3061 gccatgagga tgctggcgcg gatcagcgcg cccgtcttca aggcgtggac ctgttcgatc
     3121 tcggccaggc cgagggtccg gccttccgcg gcaaggtcca gggcctggcc acccgccatg
     3181 cccgccgtgc cgctcgcttc ggccaggatg gcgatcatcc gtactcgaat gcggtcgtcc
     3241 gggacaccgg cgtgacttgc gagaacctgg aatgcgagca cctgcagcga gtcgccggcg
     3301 agtattgccg tggcctcgtc gaacgccttg tgacaggtgg gacgaccccg gcgcaggtcg
     3361 tcgtcgtcca tcgccggcag gtcgtcgtga acgagtgaat aggcgtggat cagctccacc
     3421 gcgcaggctg cagcgtccac ggttgaaagc gccacgccga gcgtctcggc cgtcgcgtag
     3481 acgagcgccg ggcggatgcg cttgcccggc ccgagcacgc cgtagcgctg cgcggcgtgc
     3541 aggcgctgcg ggactgcgga gtcgggcggc agccagcgct cgagctcctg ctcgacccgg
     3601 ctgcgccact cgtcgaggcg cgaactcagg gagcccatcg ggcggccggc taaggctcct
     3661 cctcgaaggg ttccggttcc gcatccggag tcttcttgag caggatttcc acgcgctgct
     3721 cggcggcctt gagcgacgcc tggcagctgc gcgcgagttc gatgccgcgt tcgaactgct
     3781 tcagggcgtc ttcgagcgga agttcgccgt gttcgagtcg ctcgacgacg gcctcgagat
     3841 ccgcgagcgc ttgttcgaag tcgggcgact tgggcttctt gctggtcacg ctgttctccg
     3901 gggcgcgcaa gacttgcaca ggggttgcgg cgggtcaacg caacgcaaag tcctgaaagg
     3961 acaatagcgt cctgtccgga tcctgcgcgg ctgcgacccg tgctagcgtt gacagcatgc
     4021 cggttgcggc ctagatttgc cttcagttag accaagtcta aacgaggctc gtcgcaccgg
     4081 cgagcgccag atccgtggag gcacgcatga agaacttgaa gggaacgaag accgaaaaga
     4141 acctgaagga cgcattcgca ggcgagtcgc aggcgaaccg tcgttacctg tatttcgcgc
     4201 agaaggcgga cgtcgaggga ttcaacgacg tggccgccgt gttccgctcg acggcggaag
     4261 gcgagacggg ccatgcgcac ggccacctcg agtacctcga gcaggtcggc gacccggcca
     4321 ccggtctgcc gatcggcagc acgtcgaaca acctcaaggc gtccatcgcc ggcgagacgc
     4381 acgagtacac cgacatgtac ccgggcatgg cgaagtcggc ccgcgacgag ggattcgacg
     4441 agatcgccga ctggttcgag acgctggcca aggcagagcg ttcgcacgcg aaccgcttcc
     4501 agaaggcgct cgacgccctc tgaccggcct gtccgctgac gagtcgaagg agggcgggcc
     4561 cggcgggtcc gccctttttc gtcacgggcg aggcagccgc gcgtgggggg cgtgccacgc
     4621 tcgacggcaa ggagacgatc gatggctgaa ggaaccagga cacccgctgc cggcgaaggc
     4681 agcctcgcgg cgccgacgcg ccacccgatt gcctggcgcg agccggactt ctacgacgaa
     4741 gagtcgctct tcaaggaact gcaccgggtc tacgacctgt gccacggctg tcgccgctgc
     4801 ttcagcctgt gcaatgcgtt cccgctgctg ttcgatgcca tcgacgcgag cccgacgggc
     4861 gagctcgacg gcgtggaccg caaggtctac tgggaggtcg ccgatcactg ctacctctgc
     4921 gacatgtgct acatgacgaa gtgtccgtac gtgccgccgc acccgtggaa catcgacttc
     4981 ccgcacctga tgctgcgggc caaggcggtg aggttccgca agcagggcgc gcggctgcgc
     5041 gaccgcatcc tcacctcgac cgacgccgta ggcacgctcg ccggcatccc cgtcgtggcc
     5101 caggccgtga acctcgtgaa ccgcacgaag ttcggccgca cggtgctgga caagacgatg
     5161 ggcgtgcacc cgggcgcacc ggtccccgac tatcacagca actccggtcg caagcgggtg
     5221 gcgcgcggcg accacgcggg actcgaggtc aagcccacca cggagacgaa gggacgcgtc
     5281 gcgctgttcg cgacctgcta ctgcaaccgc aacgaacccg acatcgacgc ggaccttgcg
     5341 gccgtgttcc ggcacaacgg aattcccgtg agcgtgatcg ccagggagcg ctgctgcggc
     5401 atgccgaagc tcgagctcgg cgacctcgag gcgatcgagc aggcgaagaa cgtcaacgtg
     5461 cccgccatgg ccgcgctcgt ggattcgggg cacgacctgg tggcgccgat cccttcgtgc
     5521 gtgctcatgt tcaagcagga gctgccgttg ctctacccgg acgacccgct ggtggccaag
     5581 gtcaaggcgg cgatgttcga tccgttcgaa tacctgatgc tgcggcacaa ggcgggcctg
     5641 ctgcgcaccg acttcgtgcg gcagctcggc aaggtgagct accacgtgcc gtgccacctg
     5701 cgggtgcaga acctggggct caagacacgc gaggtgctgt cgctcattcc cggcacggaa
     5761 gtcgacgcca tcgagcgctg ctcgggacac aacggcacct acggggtcaa gcgcgagtac
     5821 cgcgacatct cgatgaagat cggcaagccg gtgatcaatc gcgtgcaggc ggcgtcagcc
     5881 gacttctacg cgagcgactg cccgatggcg gggcaccaga tcgagtccgg actcgaaaag
     5941 gcggcgggcc cgacgcaccc gctcacgctc ctgcgcatgg cgtacggcct gtgaacggcc
     6001 cgctgaccgc cgctgacctc atgagcctcg agcagtacgc gaggagccgc gccgaattcc
     6061 gccccaaggt gctggcgcac aagcgtgacc gcaccatcca gtgcgggccg aacgcgacgt
     6121 ggtgcttcga gga
//
LOCUS       DAOMFD010000117         6110 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4849_length_6110_cov_29.656152, whole genome shotgun sequence.
ACCESSION   DAOMFD010000117 DAOMFD010000000
VERSION     DAOMFD010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4849_length_6110_cov_29.656152"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(70..540)
                     /gene="moaC"
                     /locus_tag="PL152_07510"
     CDS             complement(70..540)
                     /gene="moaC"
                     /locus_tag="PL152_07510"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018465116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HQR49167.1"
                     /translation="MFSHVDDDDRPAMVDVSGKAVTKRTAVAEARVRFPSTVADALRE
                     AGFSTSKGPVFQTAIIAGVMAAKRTHELIPFCHPLGIENCGVEIDMGDGDEAVIRCAV
                     AVHHRTGVEMEALTGASVAALTVYDMCKSLSHEIVIASVRLLEKHGGRRDVSQP"
     gene            complement(544..1605)
                     /gene="moaA"
                     /locus_tag="PL152_07515"
     CDS             complement(544..1605)
                     /gene="moaA"
                     /locus_tag="PL152_07515"
                     /EC_number="4.1.99.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR02666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HQR49168.1"
                     /translation="MGRNTGDAGVAALPGRGPVDRLGRPMRDLRISVMDRCNFRCPYC
                     MPRETFHDRYRFLRASERLDFTEIARVARLATRLGVRKVRITGGEPLLRSGLADLVAE
                     VSSLDGIEDVALTTNGVLLAQHAAELKGNGLHRVTVSLDSLDPEVFARMSGGFGGLEQ
                     VLEGIDAAITAGLEPVKVNAVVKRGVNDHTVLDLLERFRGTPVIVRLIEFMDVGTRNA
                     WRAQDVVTSAELLRQVRARWPVSPVAGRTRGQVAERYRYDDGAGEIGFISSVSAPFCG
                     DCSRARLSSEGVLYTCLFATSGLDLRSPLRAGATDDDLLALLRGAWSGRADRYSEIRE
                     VERAAGHALHKVEMHYIGG"
     gene            complement(1616..2824)
                     /locus_tag="PL152_07520"
     CDS             complement(1616..2824)
                     /locus_tag="PL152_07520"
                     /inference="COORDINATES: protein motif:HMM:NF015418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HQR49169.1"
                     /translation="MGPLPTPAEAEARVLERVKPLPIESRPLASLAGAVLAQSVRAER
                     DQPPFDRVTMDGIALASSAWQAGVRRFRIAGIQAAGRRPLAVGSAADCIEVMTGAVLP
                     QGCDCVIPVELLRQADGWAEVSEDASVEPYANVHTRGLDCREGDMLLEPGTRLGAPEI
                     AVLASAGLPRADVRADPRIVIVSTGDELVDPGEPIESWQVRRSNSSALRAALATRGFH
                     RVAEDHLPDDAGVMRERLAVHLATYDVVVLSGGVSMGRYDHVPAALRELGVREVFHKV
                     AQRPGKPLWFGVAPGGQAVFGLPGNPVSALVCLVRYVVPGLLAAIGAGPRPVESVPLG
                     ASFVVRPALAYFLPVALERSPTQGTVAMPKPTRGSGDFVSLLGTDGFVELPPGPADYP
                     AGTVAAFYRW"
     gene            complement(2832..3212)
                     /locus_tag="PL152_07525"
     CDS             complement(2832..3212)
                     /locus_tag="PL152_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49170.1"
                     /translation="MTFATRLATAAALLVTGVAAHAACVYPQAPQNIPNGATATKDQM
                     LAAQAQIKEYSKAVQEVYLPCLETERKAAEAALDPGDPELAQKKLALQSMEAKKHNAA
                     LDELTAVAGRWSEEIKAFNNASKK"
     gene            3477..4331
                     /gene="phhA"
                     /locus_tag="PL152_07530"
     CDS             3477..4331
                     /gene="phhA"
                     /locus_tag="PL152_07530"
                     /EC_number="1.14.16.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004505 - phenylalanine 4-monooxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0006559 - L-phenylalanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine 4-monooxygenase"
                     /protein_id="HQR49171.1"
                     /translation="MTGPDPVVTYGASTHGVRGDYADMRPDYTVTQDYSAYDEAQHDR
                     WRRLHARQMRLVPGRACPEFLRVIDTLGYGTGIPRFDEVNERLTRATNWQVVAVPGLL
                     PEQVFFGHLAERRFPVTVWLREESEFEYIVEPDVFHDFFGHVPLLFDPVFADYMQAYG
                     RGGLKADGLHALEYLARLYWYTVEFGLIRTTEGLRIYGAGILSSPGEVEYAIGSPEPR
                     RIGFDLRRVMRTRYKIDTYQQTYFVIDDFAQLFRETAPDFTPLYEEISRLEQLPANAL
                     LPGDRVYS"
     gene            4407..5939
                     /locus_tag="PL152_07535"
     CDS             4407..5939
                     /locus_tag="PL152_07535"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0102662 - malonate-semialdehyde
                     dehydrogenase (acetylating, NAD+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HQR49172.1"
                     /translation="MIASISAARAGAAPFSLGHFVTGRPVAGASGRTGPVYNPATGEL
                     RGRVAFASADETRAAIASADAALAGWSATPPLQRARVMFRFKALLEQHADEIAALLTS
                     EHGKVLSDARGELVRGLEVVEFACGIPQLLKSEFTENVGTNIDSWSIRQPVGVCAGIT
                     PFNFPAMVPMWMFPVAIACGNTFVLKPSEKDPSVPMRVAELLKEAGLPDGVFNVVNGD
                     REAVDTLLTDPRVAAVSFVGSTPVAEYIYRTASAHGKRVQALGGAKNHMVVMPDADTR
                     NAASALVGAAYGSAGERCMAISVAVAVGDAGDRLVEEVKRQVAELKVASGVEPGADMG
                     PLVTRDHLERVSTYVELGVSEGASLVVDGRGRTVAGHEGGYFLGPCLFDHVKPDMRIY
                     REEIFGPVLCVVRLPDLAAATRLVNEHEFGNGTAIFTASGEAARTYTQGVQCGMVGVN
                     VPIPVPMAFHSFGGWKRSLFGPLHVHGPDGVRFYTRLKTVTQRWPTAQREGAQFVMPT
                     MK"
     gene            5971..6047
                     /locus_tag="PL152_07540"
     tRNA            5971..6047
                     /locus_tag="PL152_07540"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6005..6007,aa:Arg,seq:tcg)
BASE COUNT          931 a         2106 c         2143 g          930 t
ORIGIN      
        1 ccttgtcgcg gcccatgcgc cggctctcgc cgcccgcgag caccaggccg tacagtggtg
       61 cgagcgagtt catggctgcg acacgtcgcg ccggccgccg tgcttctcca gcagccgcac
      121 cgaggcgatc acaatctcgt gcgacagcga cttgcacatg tcgtacaccg tcagcgcggc
      181 gaccgacgcg ccggtgagcg cttccatctc gacgccggtg cgatggtgga ccgcgaccgc
      241 gcagcggatc acggcctcgt cgccgtcgcc catgtcgatc tcgacgccgc agttctcgat
      301 gccgagcggg tggcagaacg ggatcagctc gtgcgtgcgc ttcgcggcca tcactcccgc
      361 gatgatggcg gtctggaaca cgggcccctt gctcgtggag aatccggcct cgcgcagcgc
      421 atcggcgacc gtcgacggaa aacgcacgcg cgcctcggcg acggcggtcc gcttcgtgac
      481 ggccttgcca ctcacgtcca ccatcgccgg ccgatcgtcg tcgtccacgt ggctgaacat
      541 cgtctatccg ccgatgtagt gcatctcgac cttgtggagc gcgtgccccg cggcgcgttc
      601 cacctcgcgg atctcgctgt agcgatccgc gcgccccgac cacgcgccgc gcagcagcgc
      661 aagcaggtcg tcgtccgtcg cgccggccct gagcggcgag cggaggtcga gcccgctggt
      721 cgcgaacagg cacgtgtaga gcacgccttc ggaggacagg cgcgcccggc tgcagtcccc
      781 gcagaacggc gcggaaaccg aggatatgaa gccgatctcg cccgcgccat cgtcgtagcg
      841 atagcgctcg gccacctgcc cgcgcgtgcg ccccgccacg ggcgataccg gccagcgggc
      901 gcggacctgc cgcagcagtt cggccgacgt gacgacgtcc tgcgcgcgcc aggcattgcg
      961 cgtccccacg tccatgaatt cgatcaggcg cacgatgacc ggcgtgccgc ggaagcgctc
     1021 gagcaggtcg agcacggtgt gatcgttcac gccgcgcttg acgacggcat tgaccttgac
     1081 cggctcgaga cctgcggtga ttgccgcgtc gatgccttcg agcacctgct cgaggccgcc
     1141 gaacccgccg ctcatccgcg caaagacctc cgggtcgagc gagtcgaggc tcaccgtgac
     1201 gcggtgcagg ccgttaccct tgagctcggc cgcatgctgc gcgagcagca ccccgttcgt
     1261 ggtgagggcg acgtcctcga taccgtccag gctcgagacc tccgcgacga ggtccgccag
     1321 ccccgagcgc agcagcggct cgccgccggt gatgcggacc ttgcgcacgc cgaggcgcgt
     1381 ggcgagccgg gcgacgcgcg cgatctccgt gaagtcgagc cgttcggacg cgcgcaggaa
     1441 ccggtaccgg tcgtggaacg tctcgcgcgg catgcagtaa gggcagcgga agttgcagcg
     1501 gtccatgacc gagatgcgca ggtcgcgcat cgggcgcccg agccggtcga ccgggcctcg
     1561 acccggcagg gcggccacgc cggcatcccc ggtatttcgt cccatcgcgc agccctcacc
     1621 agcggtagaa cgccgccacc gtcccggcgg gatagtccgc cggaccgggc ggaagttcaa
     1681 caaatccgtc cgtgccgagc agggagacga aatcgcccga gccgcgggtc ggcttgggca
     1741 tggcgaccgt gccctgcgtc gggctgcgct cgagcgccac cggcaggaag tatgcgaggg
     1801 cgggccggac gacgaagctt gcgccgagcg gcaccgactc gacgggtcgc ggcccggcgc
     1861 cgatcgcggc caacagcccg gggaccacgt aacggaccag gcagacgagg gcggagacgg
     1921 gattgcccgg caatccgaag actgcctggc cgccgggcgc gacaccgaac cacagcggtt
     1981 tcccgggacg ctgggcgacc ttgtggaaca cctcgcggac gccgagctcc cgcagcgcgg
     2041 ccggcacgtg gtcgtagcgc cccatggaca cgcccccaga cagcacgacg acgtcgtagg
     2101 tcgcgaggtg cactgcgagc cgctcccgca tcactccggc gtcgtcgggc aggtggtctt
     2161 cggcgacacg atggaacccg cgcgtcgcga gcgcggcgcg caatgcgctc gagttcgagc
     2221 ggcgcacctg ccagctctcg atgggctcgc ccgggtccac gagttcgtcg cccgtcgaga
     2281 cgatgacgat gcgcggatcg gcgcggacgt cagcgcgtgg caggcccgcc gacgcgagca
     2341 ccgcgatctc gggagcgccc agccgggtgc cgggctcgag cagcatgtcg ccttcgcgac
     2401 agtcgaggcc ccgcgtgtgc acgtttgcat agggctcgac cgacgcgtcc tcgctcacct
     2461 cggcccagcc gtcggcctgg cgcagcagtt cgaccgggat cacgcagtcg cagccctgcg
     2521 gcaggaccgc gccggtcatt acttcgatgc agtcagccgc tgagccgact gccagagggc
     2581 gtcttccggc cgcctggatc cctgcgatgc ggaaacgtcg gacgccggcc tgccacgcgg
     2641 acgacgccag cgcgatgccg tccatggtga cccgatcgaa cggcggctgg tcgcgctctg
     2701 cgcggaccga ctgggcgagg actgcacccg cgagcgatgc gagcggacgc gactcgatcg
     2761 gcagtggctt cacccgctcg aggacccgcg cctcggcctc ggcgggcgtc ggcagcggac
     2821 ccatggcgaa gctacttctt gctcgcgttg ttgaacgcct tgatctcttc gctccagcgg
     2881 ccggcgacgg cggtcagctc atcgagggcg gcattgtgct tcttggcctc catcgactgg
     2941 agggccagtt tcttctgcgc aagctccggg tcacccgggt cgagcgcagc ctccgcagcc
     3001 ttgcgttcgg tctcgaggca agggaggtac acctcctgga ccgccttgga gtactccttg
     3061 atctgggcct gcgcggccag catctggtcc ttggtcgccg tcgcgccgtt ggggatgttc
     3121 tggggcgcct gcggatacac gcaggcggca tgggcggcaa cgccagtgac gagcagcgca
     3181 gcggcggtgg cgaggcgggt ggcgaacgtc atcacggtcc ctcttgcggt gatagctgga
     3241 gcaaacggct caacggctgg gtcacatggg cgcggaacta tagcagacgg acctgcggtg
     3301 cccgagcagc gcccatgtgc tcgagcagtc aggttggacc gcaatctggc gcggaagtgc
     3361 cccggctccg ggcgctcgtc cgggtcgcgc ccgtgatcac cagcggcgcc cgggagcccc
     3421 gcgcgactcg gcatgaggct atgcttggcg ccacccaatc acggggggag acccggatga
     3481 caggaccgga cccggtcgtg acctacggcg ccagtaccca cggggtgcgc ggcgactatg
     3541 ccgacatgcg cccggactac accgtcaccc aggactactc ggcctacgac gaggcccagc
     3601 acgaccgctg gcgacgcctg cacgcgcggc agatgcgact ggtgccgggg cgcgcctgcc
     3661 ccgagttcct gcgcgtgatc gacacgctcg gctacggcac cggcatcccg cgcttcgacg
     3721 aggtgaatga acgcctcacg cgcgccacga actggcaggt ggtggccgtg ccgggcctgc
     3781 tcccggagca ggtcttcttc gggcacctcg cggagcggcg atttcccgtc accgtctggc
     3841 tgcgcgagga gagcgaattc gagtacatcg tcgagcccga cgtcttccac gacttcttcg
     3901 gccacgtgcc gctgctgttc gaccctgtct tcgcggatta catgcaggcc tatgggcgcg
     3961 gcggactgaa ggccgatggg ctccacgcgc tcgagtacct ggcgcggctc tactggtaca
     4021 cggtcgagtt cgggctgatc cgcacgaccg aggggctgcg gatctacggc gcggggatcc
     4081 tctcgtcacc gggcgaggtc gagtacgcga tcggcagtcc cgagccgcgg cgcatcggct
     4141 tcgacctgag gcgagtcatg cgcacgcgct acaagatcga cacctaccag cagacctact
     4201 tcgtgatcga cgacttcgcg cagctcttcc gcgagacggc gcccgatttc acgccgctgt
     4261 acgaggagat ctcccgcctg gagcagcttc cggccaatgc gctgctgccc ggggaccgcg
     4321 tgtacagctg actggcgggc aggggccgga tggtgtccaa tgacgtcccc gggccaggcc
     4381 cggcgtccat ccgcagagga ttcacgatga tcgcatcgat ttccgccgcc cgtgccggcg
     4441 cggcgccgtt ttcactggga catttcgtga ccggccggcc tgtggccggc gcgtccggcc
     4501 gcaccgggcc ggtctacaac ccggcaaccg gcgagctgcg cggtcgcgtg gccttcgcca
     4561 gtgccgacga gacccgcgcc gccattgcgt cggccgacgc cgcgctggcc ggctggtctg
     4621 cgacaccacc gctgcagcga gcgcgcgtga tgttccgctt caaggcgctg ctcgagcagc
     4681 acgccgacga gatcgcggcg ctgctcacgt ccgagcatgg caaggtgctg tcggacgcgc
     4741 gcggcgaact cgtgcgtggc ctcgaggtgg tcgagttcgc ctgcggcatc ccgcagctgc
     4801 tgaaaagcga gttcaccgag aatgtcggga ccaacatcga cagctggtcg atccgccagc
     4861 ccgtcggcgt gtgcgctggc atcacgccgt tcaatttccc ggcgatggtg ccgatgtgga
     4921 tgttcccggt ggcgatcgcg tgcggcaaca cgttcgtgct caagccgtcg gagaaggacc
     4981 ccagcgtgcc gatgcgcgtc gcggagctgc tgaaggaagc cggactgccc gacggggtgt
     5041 tcaacgtcgt caacggcgac cgcgaggccg tcgacacgct gctcaccgac ccgcgggtcg
     5101 cggccgtgag tttcgtcggc tcgacgccgg tggccgagta catctaccgg acggcgtccg
     5161 cgcacggcaa gcgcgtgcag gcgctcggcg gcgccaagaa ccacatggtc gtgatgcccg
     5221 acgccgatac gcgcaatgcc gccagcgcgc tcgtgggcgc cgcgtacggc tcggccggcg
     5281 agcgctgcat ggcgatctcg gtggccgtgg cggtgggcga tgcgggtgat cgcctcgtcg
     5341 aggaagtgaa gcgccaggtc gccgagctca aggtggcatc gggcgtcgag ccgggtgcgg
     5401 acatggggcc gctggtgacc cgcgaccacc tcgagcgtgt aagcacgtac gtggagctgg
     5461 gcgtcagcga gggcgcgtcg ctggtcgtgg atggccgtgg gcggaccgtt gccggccacg
     5521 agggagggta tttcctgggt ccatgcctgt tcgatcacgt taagcccgac atgcgcatct
     5581 accgggaaga gatcttcggg ccggtgctgt gcgtggtgcg cttgccggac ctcgcggcgg
     5641 cgacacggct cgtcaatgag cacgagttcg gcaatggcac tgcgatcttc acggcgagcg
     5701 gcgaggcggc gcgcacctac acccagggcg tgcagtgcgg catggtgggc gtgaacgtgc
     5761 cgattcccgt gccgatggcg ttccactcgt tcgggggctg gaagcgctcg ctgttcgggc
     5821 cgctgcacgt gcacgggccg gacggggtgc ggttctacac ccggctcaag accgtgaccc
     5881 agagatggcc gaccgctcag cgtgaaggcg cgcagttcgt gatgccgacg atgaagtaac
     5941 ggcactgcgc tagaattcac ttcctcccgc gcggccgtag ctcagttgga tagagcatca
     6001 ggcttcgaac ctgagggtcg ggggttcgaa tccctccggc cgcgccagtg aaacggggtg
     6061 tattccgatc acatggttta cacctcattc cggacacatg gttaacactg
//
LOCUS       DAOMFD010000118         6075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4906_length_6075_cov_32.059635, whole genome shotgun sequence.
ACCESSION   DAOMFD010000118 DAOMFD010000000
VERSION     DAOMFD010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4906_length_6075_cov_32.059635"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="PL152_07545"
     CDS             <1..297
                     /locus_tag="PL152_07545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HQR49173.1"
                     /translation="TVSWYGGQFHHRPTASGEIFNANGLTMAHPTLPFGTKVKVTNLR
                     NGRSVVVRVNDRGPFIGHRIADLSRGAAQVLGMMQRGVAHARIEILDDGESITD"
     gene            318..1274
                     /locus_tag="PL152_07550"
     CDS             318..1274
                     /locus_tag="PL152_07550"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR49174.1"
                     /translation="MPRRTAPVEGDTLGQKLVAALILRSLRLSRRAGLRGDSPVGRQR
                     RLIRTLAKATIVPGASTFAPGSVGGVPGEWAAPRSGQQSERRIVLYLHGGAYCIGSPA
                     THRALTGRLALASSARVLALDYRLAPEHPFPAALEDACAAYDGLLASGSQPADIVIAG
                     DSAGGGLSVATALRLRSLGRPPPAALLLFCPWADLRPRQSAAGLSATDEFPRDWITRD
                     WTNACADMYLSGHDPTDPYASPLHGELAGLPPTLIQVGGDDTLREDAERLHARMRAAG
                     VDVCLSVYPRRWHDFQLHGGLLRDAHRAVDEAAAHVRAHACR"
     gene            complement(1158..2174)
                     /locus_tag="PL152_07555"
     CDS             complement(1158..2174)
                     /locus_tag="PL152_07555"
                     /inference="COORDINATES: protein motif:HMM:NF019474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR49175.1"
                     /translation="MPSLRARLTNAFLRVTTKSMWKPGLDIHKIRVHSAKMDARIARK
                     PLPIATEPAEVAGVKATWYGDRSLEANGTILYLHGGAWCLHLPAIFSRHAARLSQLTG
                     MRILLPDYRLAPEHPFPAGVDDCYTAYRWLVEHGYAQRPLAVAGDSAGGSLTLTTLMQ
                     ARDAGLPLPNCAVMLSPSTDLLPSAPSWTYNSAADPMFGPGAVDLLPKIYCPGMDRTN
                     PLLSPLFGKWHGLPPLLFHAGSTEMILDDSVRAHDRARQAGVAAEIEVWIEMPHVFQV
                     FSWLPESKLAMRRIADFIVAHARRSAAGVGANVGGRLVDGAVRVAQQAPVQLEIVPPA
                     RIDA"
     gene            2294..2911
                     /locus_tag="PL152_07560"
     CDS             2294..2911
                     /locus_tag="PL152_07560"
                     /inference="COORDINATES: protein motif:HMM:NF024922.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HQR49176.1"
                     /translation="MSNTEPAAAVTPRVFELPDAEVALWPAAFAADEAAYLFDELRVG
                     IDWQEEEVVIFGQRRRVPRLVAWHGDPGASYTYSGTLHEPLRWTPPLLQVRRRVEELA
                     GRQFNAVLLNRYRDGRDGMGWHADDEPELGSDPNIASVSLGAVRRFCLRHRRRKDAKL
                     ELRLPQGSLLLMSGATQHHWVHAVPKTASAVGERINLTFRRVGLR"
     gene            2981..3367
                     /locus_tag="PL152_07565"
     CDS             2981..3367
                     /locus_tag="PL152_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49177.1"
                     /translation="MRPMTFVCLCFTALALASTAGCARKQEQVSYQKDVAPILQAKCA
                     ACHVPGQQGYVASGFDLSGYDSLMKGTKFGAVVIPGDPLTSVLVMLIEGRADPSLKMP
                     HGGAQPLTKDEIKTIRRWVKQGAKNN"
     gene            3581..6058
                     /locus_tag="PL152_07570"
     CDS             3581..6058
                     /locus_tag="PL152_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR49178.1"
                     /translation="MAALAGGVGSFAAPRVALAQQEVEQPQGVLEEVVVNAQRSNQAL
                     QDVPISVQVVDSDLINDTAATNMNDLNGFIPGLVVSGGSPTQPKYQLRGIQTGDFGIG
                     TDPAVGVYLDGIYSARSGASLTTFNDIDHVEVLKGPQGTLLGRNSAAGAVSIYTKQPT
                     DKWEGNVRARMGDYGTQLYSGVLNAPITDGLALRTTGVYNSSDGWIKDAATHKELNPT
                     EEYSFRTALRWDIAEATSATLTWDYDNLDQLARPAIGIVPVSDTYPFVPFFSPPGELP
                     ANYSDTYVNPLKAKVYNDVVKNDESRLLNRYNLFIDHDFGWANFRSSTSYTKFNTNNR
                     EDEDGTNLRPYLYFDTANIEHNQSWYQEFKFSHSDEKFDWVAGVSYYSEDAKQTSAAH
                     TYTDSVDRALVNLGYAGAPGLFAGTDFLYNNFFVPYVLDNFGTQLPALATTGQTWDES
                     MYNHGEYSAVAVFGDTIWHATDRLNLTFGLRYTSDDKHFSWLNGPRVAPGADAALAEM
                     EAYGFFDWIALAHAADPDNVPLLTPDMYQNDLVFVNNPAIEGQKIKKHDTWSDWSPRL
                     VADYKYTDNVMFWGSIAKGYKAGGYNSVEIGSVFDPEDVWNYEAGVKSVFPEINLLVN
                     SSVYYYIYKDKQAISLVTQTEGVPKYLIDTSDEQAWGVDLDARWQPIPDLTLTGTVAW
                     IDATYASKKTAEGVDLSGDPTGEPYWSLSLGADYVVSLGEYGHTDFSAQWGYRGETRC
                     NAGATLQGTCQVSPNFKVGESQQRLDLRAAWTSDSERWGAAVFVTNVFDDQYVDGVNN
                     LTTSTFGTPFASITAPRQWGVEALYQF"
BASE COUNT         1048 a         2052 c         2039 g          936 t
ORIGIN      
        1 acggtgtcgt ggtacggcgg gcagttccac caccgcccga cggccagcgg cgagatcttc
       61 aatgccaatg gcctgacgat ggcccacccg acgctgcctt tcggcaccaa ggtgaaggtc
      121 acgaacctgc gcaatggccg cagcgtggtc gttcgggtca acgatcgagg cccgttcatc
      181 gggcaccgga tcgccgacct gtcgcggggg gccgcccagg tgctcggcat gatgcagcgc
      241 ggcgtcgccc acgcccgcat cgagatcctg gacgacggcg agtccatcac ggactgaccc
      301 tggccgacct cccagcgatg ccgcggcgca ccgctccggt cgagggcgac accctcggcc
      361 agaagctggt tgccgcattg atcctccgct ccctgcggct cagccgtcgt gccggcttgc
      421 gtggcgacag tcccgtcggc cgccagcgcc gcctcatccg gacgctcgcg aaagcgacga
      481 tcgtgccagg agcctcgacc ttcgccccgg gctcggtcgg tggcgtgccg ggtgaatggg
      541 cggcgccaag atccggtcag cagtctgaac ggcggatcgt gctgtacctg cacggcgggg
      601 cgtactgcat cggctcgccc gcgacgcatc gcgcgctgac cggccgcctc gcgctcgcga
      661 gcagcgcgcg cgtcctggcc ctggactatc gcctcgcccc tgagcatccg ttcccggccg
      721 cgctcgagga cgcgtgtgct gcttacgacg gactgctcgc cagcggctcg caacctgccg
      781 acatcgtcat tgcgggcgat tccgcgggcg gcggactgag cgtcgccacc gcgctcaggt
      841 tgcgatcgct gggtcggccg ccgcccgccg cgctgctgct cttctgtccc tgggcggact
      901 tgcgcccgcg gcaatcagcc gcggggctgt cggcgacgga tgaattccct cgcgactgga
      961 tcactcgtga ctggaccaat gcgtgcgcag acatgtattt gtcgggacac gaccccacgg
     1021 atccgtacgc gtcgccgctg cacggcgaac tggccggact gccacccact ctcatccagg
     1081 tcggtggcga cgacacactg cgtgaggatg ccgaacgcct gcacgcacgg atgcgcgccg
     1141 ccggcgtgga cgtgtgctta agcgtctatc cgcgccggtg gcacgatttc cagctgcacg
     1201 ggggcctgct gcgcgacgcg caccgcgccg tcgacgaggc ggccgcccac gttcgcgccc
     1261 acgcctgccg ctgacctgcg cgcgtgcgcc acgatgaagt ccgcgatcct gcgcatcgcg
     1321 agcttcgatt ccggcagcca gctgaacacc tggaacacat gcggcatctc gatccagacc
     1381 tcgatctcgg cggccacacc cgcctggcgc gcgcggtcgt gcgcgcgcac cgagtcgtcg
     1441 aggatcatct ccgtggagcc tgcgtggaac agcagcggcg gaaggccgtg ccacttgccg
     1501 aacagcgggg acagcagcgg attggtgcgg tccatgccgg ggcaatagat cttcggcaac
     1561 aggtctaccg cgcctggtcc gaacatcggg tcagcggcgg agttgtacgt ccacgacggg
     1621 gccgatggca gcaggtccgt ggacggcgag agcatcaccg cgcaattcgg cagcggcagc
     1681 cccgcgtccc gcgcctgcat caacgtcgtg agcgtcaggc tgccgcccgc cgaatcaccg
     1741 gcgacggcga gcggtcgctg cgcgtaaccg tgttccacca gccagcgata cgccgtgtag
     1801 cagtcgtcga ccccggcggg gaacggatgc tccggcgcca gccggtagtc cggcagcagg
     1861 atgcgcatgc cggtgagctg cgacagccgc gccgcgtgcc ggctgaagat cgccggcagg
     1921 tgcaggcacc acgcgccacc gtgcaggtac aggatcgtgc cgttcgcttc gagcgagcgg
     1981 tctccgtacc aagttgcctt aacgccggcg acctccgctg gctcggtcgc gatcggcagc
     2041 ggcttgcggg cgatgcgggc gtccatcttc gccgagtgga cgcggatctt gtggatgtcg
     2101 agaccgggct tccacatcga cttggtcgtg acgcggagga aggcgttcgt cagcctggcg
     2161 cgcaagctcg gcatggtcgg cacccgtatc gcatgttgca gcgcggcatt ctgcggcccg
     2221 cggcgccgtt gcgccagttc gtgaatatca cggctgctcg tgccgggagc cggccccgag
     2281 ttacgctgcc gcgatgagca ataccgagcc tgccgccgct gtcacaccgc gggtgttcga
     2341 actgccggac gcggaggtgg cgctctggcc ggcagccttc gcggcggacg aggcggcgta
     2401 cctgttcgac gagctgaggg tcgggatcga ctggcaggag gaggaggtcg tcatcttcgg
     2461 ccagcgccgc cgcgtgccgc ggctcgtggc atggcacggc gacccgggcg cgagctacac
     2521 gtactcgggc acgctgcacg agcccttgcg ctggacgcca ccgctcctgc aggttcgtag
     2581 gcgggtcgag gaactcgccg gccggcagtt caacgccgtg ctgctcaatc ggtatcgcga
     2641 cgggcgcgac ggcatgggct ggcacgccga cgacgagccc gaattggggt ctgaccccaa
     2701 tatcgcgtcg gtgtcgctcg gtgccgtgcg gcgtttctgc ctgcggcatc gccggcgcaa
     2761 ggatgcgaag ctcgaactcc ggttgccgca aggcagcctg ctcctgatgt cgggtgcgac
     2821 gcagcatcac tgggtacatg ccgtgcccaa gaccgcgagt gccgtcgggg aacgcatcaa
     2881 cctgaccttt cgacgggtcg ggcttaggta gaacagcgga ttgtcgctgc ccgccggcgg
     2941 gatcagcgtc gcggggttcg tcgacgaacc ggagaccgcc atgcgtccca tgaccttcgt
     3001 gtgcctgtgc ttcactgcgt tggcattggc ttccaccgct ggttgtgccc gcaagcagga
     3061 gcaggtcagc taccagaagg acgtcgcgcc gatcctccag gcgaagtgcg cggcctgcca
     3121 tgtgcccgga cagcagggct acgttgcgag cggattcgac ctgagcggct acgactcgct
     3181 gatgaaaggc acgaagttcg gcgccgtcgt gatcccgggc gacccgctca cgagcgttct
     3241 cgtcatgctg atcgaaggcc gggctgaccc gtcgctcaag atgccgcacg gcggggccca
     3301 gccgctcacc aaggatgaga tcaagacgat ccgccgctgg gtgaagcagg gcgcgaagaa
     3361 caattgatgc cccctcgcga cgaggggtta ggcggcattt ccgccgccgc agcgacaatt
     3421 ttgcaaaaaa acaacaatcg cgctgctaga atcgcgccgc tttagcaggc ggccggcgac
     3481 ggccatggat cgtcaataac gcaggcgacc ggcactggac cgcgccgccg catggggtgg
     3541 aataatgaac cttcgtagga ctacggtcgc gcgggccgtc atggccgcgc ttgcgggtgg
     3601 cgtcgggtcg ttcgcggcgc cgcgtgtcgc actggcacaa caggaagtcg agcagccaca
     3661 gggcgtgctc gaggaagtgg tcgtcaacgc gcagcgcagt aaccaggccc tgcaggacgt
     3721 gccgatcagc gtccaggtcg tcgacagcga cctgatcaac gacaccgccg cgaccaacat
     3781 gaacgacctg aacgggttca ttcccggcct cgtcgtgtcc ggcggcagcc cgacgcagcc
     3841 gaagtaccag ctgcgcggca tccagaccgg cgacttcggg atcggcacgg acccggcggt
     3901 cggcgtatac ctggacggca tctattcggc gcgctcgggc gcgtcgctga cgacgttcaa
     3961 cgacatcgac cacgtcgagg tgctcaaggg cccgcagggg acgctgcttg gccgcaacag
     4021 cgccgcgggc gccgtgtcga tctacacgaa gcagccgacc gacaagtggg aaggcaacgt
     4081 gcgcgcgcgc atgggcgact acgggaccca gctctacagc ggcgtgctga acgccccgat
     4141 tacggacggg ctcgcgctgc gcacgaccgg cgtctacaac tcgagcgacg gctggatcaa
     4201 ggacgcggcg acgcacaagg agctcaaccc gaccgaggag tactcgttcc gcacggcgct
     4261 gcgctgggac atcgccgagg ccaccagtgc cacgctgacc tgggactacg acaacctcga
     4321 ccagctggcg cgcccggcga tcggcatcgt gccggtgtcg gacacctacc cgttcgtccc
     4381 gttcttctcg ccgccgggcg agctgcccgc gaactactcg gacacgtacg tgaatccgct
     4441 caaggcgaag gtctacaacg acgtcgtcaa gaacgacgag tcgcgcctgc tcaatcgcta
     4501 caacctgttc atcgaccacg acttcggctg ggcgaacttc cgctcgtcca cgtcgtacac
     4561 caagttcaac acgaacaacc gcgaggacga ggacggcacg aacctcaggc cgtacctcta
     4621 cttcgacacc gcgaacatcg agcacaacca gagctggtac caggagttca agttctcgca
     4681 ctccgacgag aagttcgact gggtcgccgg agtgagctac tactccgagg acgcgaagca
     4741 gaccagcgcc gcgcacacgt acaccgacag cgtggacagg gcattggtga acctcggata
     4801 cgccggtgcg cccgggttgt tcgcaggcac ggatttcctc tacaacaact tcttcgtgcc
     4861 gtacgtgctg gacaacttcg gcacgcagct tccggcgctc gccacgaccg gccagacgtg
     4921 ggacgagtcg atgtacaacc acggcgagta ctcggcggtg gcggtgttcg gcgacaccat
     4981 ctggcacgcg accgacaggc tgaacctgac attcggcctg cgctacacga gcgacgacaa
     5041 gcacttcagc tggctgaacg gcccgcgcgt cgcgccgggc gccgacgccg cgctggccga
     5101 gatggaggcc tacgggttct tcgactggat cgcgctggca cacgccgcgg acccggacaa
     5161 cgtgccgctg ctgacgccag acatgtacca gaatgacctg gtgttcgtga acaatccggc
     5221 catcgagggc cagaagatca agaagcacga cacctggagc gactggagcc cgcgcctggt
     5281 ggcggactac aagtacacgg acaacgtgat gttctggggc tccatcgcca agggctacaa
     5341 ggccggcggc tacaacagcg tcgagatcgg ctcggtgttc gatcccgagg acgtgtggaa
     5401 ctacgaggcg ggcgtgaaga gtgtcttccc ggagatcaac ctcctcgtca actcgtcggt
     5461 gtactactac atctacaagg acaagcaggc gatctcgctc gtcacgcaga ccgaaggggt
     5521 tccgaagtac ctgatcgaca ccagcgacga acaggcctgg ggcgtcgacc tcgatgcgcg
     5581 ctggcagccg atccccgacc tcacgctcac cggcaccgtc gcctggatcg acgccaccta
     5641 cgccagcaag aagacggccg agggggtgga tctgtcgggt gacccgaccg gcgagcccta
     5701 ctggtccctg tcgctcggcg cggactacgt cgtcagtctc ggcgagtacg gccacaccga
     5761 cttctcggcg cagtggggtt accgcggcga gacgcgctgc aatgccggcg cgacgctgca
     5821 gggcacctgc caggtcagcc cgaacttcaa ggtgggcgag tcgcagcagc ggctggacct
     5881 gcgtgccgcc tggacgagcg acagcgagcg ctggggcgcc gctgtgttcg tgacgaacgt
     5941 gttcgatgac cagtacgtgg acggcgtcaa caacctgacc accagcacgt tcgggacgcc
     6001 gttcgcgtcc atcaccgcgc cgcgccagtg gggcgtcgag gcgctgtacc agttctgacg
     6061 ttgggggagc tgaga
//
LOCUS       DAOMFD010000119         6071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_4914_length_6071_cov_37.088597, whole genome shotgun sequence.
ACCESSION   DAOMFD010000119 DAOMFD010000000
VERSION     DAOMFD010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6071
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4914_length_6071_cov_37.088597"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="PL152_07575"
     CDS             <1..594
                     /locus_tag="PL152_07575"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HQR49179.1"
                     /translation="LYGLVLAGGESRRMGRDKATLAYRGRTQLETAYSLVARHCERAF
                     VSVRGDQVSEPARAALPQIVDVHDGIGPIAGIAAAQAAHPEVAWLVVACDLPFLADGT
                     VAELIARRGAHPVTAYRSAHDGLPEPLCAIYEPAMRASVLEAIAQGRNCPRKLVIASG
                     VPLLEQSDPASLDNVNTPEEFASAAARVASAPRGAHR"
     gene            591..836
                     /locus_tag="PL152_07580"
     CDS             591..836
                     /locus_tag="PL152_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HQR49180.1"
                     /translation="MTRRIHVQYFAALREQAGRGSETVATTAPDAAALYDELRANRGL
                     RLPRSMLKVAVNEEFASWDRTLADGDRVVFLPPVAGG"
     gene            836..2374
                     /locus_tag="PL152_07585"
     CDS             836..2374
                     /locus_tag="PL152_07585"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HQR49181.1"
                     /translation="MRAFSFSSAPLEPERYRAELGDPACGGYASFEGWVRDHNEGHRV
                     SRLEYEAFEALALKEGERIVAAAIEKFGVRRAACVHRVGDLAIGELAVWVGVSAAHRD
                     EAFAACRYIIDEVKHRVPIWKKEHYEDGDSGWVNCERCAHGAHDEHDASSGPGGFRHD
                     YSRQTVLPEVGEEGQRRLRAASVLVIGAGGLGVPVLQYLAAAGVGRIAIMDHDRVEAS
                     NLHRQPLYGIDDIGQPKALVAAERLARITTEVHFEAIVARAEPGNLAELIGHYDLTVE
                     CTDNLRTKFVVNDAAVRAGRPAIFASVYQYEGQLQAYLPEPDWPCVRCLWPDAPRDGL
                     VGNCAEAGVLGPVPATLGSMQAMLALRILLDLEPRPVRGLVVMDLLTFETRSIAAARN
                     PDCDHGSAGAAATHAGTGGLAEAGDLEVAFADLAAARAAGFEVVDVREEWELRMDRPG
                     RDTGLHLPLSELLDGRRSFPAVGRHLIVCAHGVRSLALAEHLREQGRPEVYSLRGGIA
                     AIDD"
     gene            complement(2443..3711)
                     /locus_tag="PL152_07590"
     CDS             complement(2443..3711)
                     /locus_tag="PL152_07590"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: protein motif:HMM:NF001221.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HQR49182.1"
                     /translation="MSGGHLAEVMDAMGRAAVDAAAVLALSSRQQKDAALLATAGSLR
                     RRSAEILAANARDMDAARGAGLGTAALDRLKLDAARVEAMAAGVEQVAALPDPIGTVI
                     TEWERPNGLRIQRVRVPLGVIGIIYESRPNVTCDAGSLCVKSGNAAILRGGSESRHSN
                     AAIHACLLDGLREGGLPDACIQLVPTTDRAAVGYMLGAMTDWIDVVVPRGGKSLVARV
                     QQEARVPVIGHLEGNCHVYVDRGADLRMAREIVRNAKLRRTGICGAAETLLVDEACVA
                     THLAPIVKDLLDAGCEVRGDARVQAVDARVRPANEDDWYTEYLDAVIAARVVDGVDEA
                     VRHIAKYGSAHTESIVTDDAAAAEAFLTRVDSAIVLHNASTQFADGGEFGMGAEIGIS
                     TDRFHARGPVGVEQLTSYKYVVRGSGQLRP"
     gene            complement(3708..4844)
                     /gene="proB"
                     /locus_tag="PL152_07595"
     CDS             complement(3708..4844)
                     /gene="proB"
                     /locus_tag="PL152_07595"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HQR49183.1"
                     /translation="MTPSAVAGRSRLAAARRVVVKVGSALLVEQSSGRVNRIWLESLA
                     EDIARLRARGQEVVLVSSGAIALGRRQLGLPAGKLELEQSQAAAAVGQIRLAHAWKEV
                     LEHHGCTVAQVLLTFGDTEQRRRYLNARNTLTTLLKLGAIPVINENDTVATAEIRYGD
                     NDRLAARVAQMASADCLVLLSDVDGLYTADPTREPDARFIAEVPRITADIEAMAGGSA
                     SDVGSGGMTTKVLAAKIAVAAGCHMCVAAGREPHPLRRIESGARCTWFVADGSPVAAR
                     KQWIAGMLKPAGELKVDDGAARALQQGKSLLPAGVTAVTGRFDRGDAVVIRDAEGREI
                     ARGLAAYSSSDAERMCGRRSAELEELLGYRGRDEMIHRDDLVLT"
     gene            complement(4841..>6071)
                     /locus_tag="PL152_07600"
     CDS             complement(4841..>6071)
                     /locus_tag="PL152_07600"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQR49184.1"
                     /translation="TGTKIFITAGEHDLTDNIVHLVLARLPDAPPGTKGISLFVVPKM
                     LPDADGKVGPANGVTCGSIEHKMGIIASPTCVMNFDESVGWLLGKENEGLSCMFTMMN
                     HARLGVGLQGQGLSELAWQAAAAYASERIQGRSLTGPKQKEKPADPIIVHPDVRRMLL
                     TQKAFVEGGRVLAFLTGREIDGSHMNPDPDARRRSSDLVAFLTPIVKAFLTDIAVEVA
                     SLGIQVHGGHGYIRETGVEQIMRDARITPIYEGTNGIQALDLLGRKVFGTQGKSQQMM
                     VGRIRETIEKFAPLPEVAPMAAELGKRLEQWNKLTLELGMAAMKNPDEAGAAATDYLA
                     FAGYVCMGWCWLASAGVAAKKLAAGAPDADFYKAKLVTARFYFDRLLPRVDAHAAAAR
                     AGAAGVMGLPAEHFNFA"
BASE COUNT          859 a         2166 c         2133 g          913 t
ORIGIN      
        1 ctgtacggcc tggtgctcgc gggcggcgag agccggcgca tgggccgcga caaggcgacc
       61 ctcgcctatc gcggccggac gcagctcgag accgcgtact cgctcgtggc gcgccattgc
      121 gagcgcgcat tcgtgtcggt gcgcggtgac caggtgagcg agcccgcgcg cgccgcactg
      181 ccgcagatcg tcgacgtgca cgacggcatc gggccgatcg ccggcatcgc ggccgcgcag
      241 gccgcgcacc ccgaggtcgc ctggctcgtc gtcgcctgcg acctgccatt cctcgccgac
      301 gggaccgttg ccgagctcat cgcgcggcgt ggcgcgcatc ccgtgacggc gtatcgcagc
      361 gcgcacgacg ggctgcccga gccgctctgc gcgatctacg agccggcgat gcgcgcatcg
      421 gtcctcgagg ccatcgccca gggccgcaac tgcccgcgca agctcgtgat cgcgagcggc
      481 gtgccactgc tcgagcaatc ggacccggcg tcgctcgaca acgtgaatac acccgaggaa
      541 ttcgcgtcgg ccgctgcgcg cgtcgccagc gcaccgcgag gtgcacaccg atgacgcgcc
      601 ggatccacgt gcagtatttc gcagcgctgc gtgagcaggc gggccgggga tcggagaccg
      661 tggcgacgac ggctcccgac gctgcggcgc tgtacgacga gcttcgggcg aaccgcgggt
      721 tgcgcctgcc acgctccatg ctcaaggtcg ccgtgaacga agagttcgcg agctgggatc
      781 gcacgctcgc ggacggcgac cgtgtcgtgt tccttccgcc ggtcgcgggg ggctgatgcg
      841 cgcgttctcg ttcagcagcg cgccgctcga accggagcgc taccgcgcgg agctgggcga
      901 tccggcctgc ggcggatacg caagcttcga aggctgggtg cgcgaccaca acgagggcca
      961 ccgggtcagc cgcctcgagt acgaggcgtt cgaagcactc gccctcaagg agggcgaacg
     1021 catcgtggcc gcggccatcg agaagttcgg cgtccggcgt gcggcctgcg tgcaccgggt
     1081 cggagacctg gccatcggcg aactggcggt ctgggtcggc gtgagcgcgg cccatcgcga
     1141 cgaggccttc gccgcgtgcc gctacatcat cgacgaggtc aagcaccggg tcccgatctg
     1201 gaagaaggag cactacgagg acggcgactc ggggtgggtg aattgcgagc gctgcgcaca
     1261 cggcgcgcac gacgagcatg acgcgtcctc cgggccgggc ggcttccggc acgattactc
     1321 ccgccagacc gtgctgccgg aggtcggtga ggaaggacag cggaggctgc gcgccgcttc
     1381 ggtcctcgtg atcggcgccg gcggactcgg cgtgccggtg ctgcagtacc tcgcggctgc
     1441 gggtgtcggc cgcatcgcga tcatggatca cgaccgcgtc gaggcgagca acctgcatcg
     1501 ccagccgctg tacggaatcg acgacatcgg gcagccgaag gcgctcgtcg ccgccgagag
     1561 gctggcgcgc atcacgaccg aggtccattt cgaagccatc gtcgcccgcg ccgagccggg
     1621 caatctggcg gagctcatcg ggcactacga tctcaccgtt gagtgcaccg acaacctgcg
     1681 caccaagttc gtcgtgaacg atgcggcggt tcgtgctgga cggcctgcga tcttcgcgag
     1741 cgtgtaccag tacgagggac agctgcaggc gtacctgccg gagcccgact ggccatgcgt
     1801 gcgttgcctc tggcccgacg cacccaggga cggcctcgtc ggaaactgtg ccgaggccgg
     1861 cgtgctgggc ccggtgccgg cgacgctggg ctcgatgcag gcgatgctcg cgctgcgcat
     1921 cctgctcgac ctcgagccgc ggcccgtgcg cgggctcgtc gtcatggacc tgctcacgtt
     1981 cgagacgcgc agtatcgccg ctgccaggaa ccccgactgc gatcatggat ccgcgggcgc
     2041 agcggcgacg cacgccggga ccggcggact cgccgaggcc ggggacctcg aagtggcttt
     2101 cgcggacctc gcagccgcgc gcgccgccgg gttcgaggtg gtggacgtgc gcgaggagtg
     2161 ggaactcagg atggaccgtc ccgggcgcga caccggcctg cacctgccgc tgagcgagct
     2221 gctcgacggt cgacgctcgt tcccggcggt cgggcgccac ctcatcgtct gtgcgcatgg
     2281 cgtgcgcagc ctcgccctcg ccgagcacct gcgcgagcag ggtcgccccg aggtctattc
     2341 actgcgcggc ggcatcgcgg ccatcgacga ttgaaggccg cggcgtcggc gcgtcgccct
     2401 gcgtgaactc gccccggtga ggacttgcgt ccagggtggt cgttacggcc gcagctggcc
     2461 cgagccgcgc acgacgtact tgtagctcgt gagctgctcg acgccgaccg gcccgcgcgc
     2521 gtggaaacgg tcggtggaga tgccgatctc cgcacccatc ccgaactcgc cgccgtccgc
     2581 gaactgggtc gaggcgttgt gcagcacgat cgcgctgtcg acgcgcgtga ggaacgcctc
     2641 ggcagcggcc gcatcgtcgg tgacgatcga ctcggtgtgg gcggaaccgt acttcgcgat
     2701 gtgacggacc gcctcgtcca cgccgtcgac gacgcgtgcg gcgatgacgg catcgaggta
     2761 ttccgtgtac cagtcgtcct cgttcgccgg ccggacacgc gcgtccacgg cctgcacgcg
     2821 cgcatctccg cgcacttcgc agcctgcatc gagcaggtcc ttcacgatgg gcgcgaggtg
     2881 cgtggcgacg caggcctcgt ccacgagcag cgtctcggcc gcaccgcaga taccggtgcg
     2941 ccgcagcttc gcattgcgca cgatttcccg tgccatgcgc aggtcggcgc cgcgatcgac
     3001 atacacgtgg cagttgccct ccaggtggcc gatgaccggc acccgcgcct cctgctgcac
     3061 gcgcgcgacg aggctcttgc cgccccgcgg caccaccacg tcgatccagt ccgtcatcgc
     3121 gccgagcatg tagcccacgg ccgcgcgatc ggtcgtcggg acgagctgga tgcaggcatc
     3181 cggcagcccg ccctcgcgga gtccgtcgag caggcaggcg tggatcgcgg cattcgaatg
     3241 ccggctctcg cttccgccgc gcaggatcgc cgcattgccg gacttgacgc acaggctgcc
     3301 ggcatcgcag gtcacgttgg gccggctctc gtagatgatg ccgatcacgc cgagaggcac
     3361 gcgcacccgc tggatacgca ggccgttcgg cctctcccac tcggtgatca ccgtgccgat
     3421 gggatcgggc agcgcggcca cctgctcgac gcccgcggcc atcgcctcga cgcgcgcggc
     3481 atcgagtttc aggcggtcga gcgcggccgt acccagtccg gcaccacgcg cggcatccat
     3541 gtcccgtgca ttggccgcga gaatctccgc cgagcgccgt cgcagcgatc ccgccgtcgc
     3601 gagcagcgcg gcatccttct gctgtcgcga cgacagcgcc aggacggccg ctgcatccac
     3661 ggcggcgcgt cccatggcgt ccatcacctc ggcaaggtgg ccgccgctca cgtgagcacc
     3721 aggtcgtcgc ggtggatcat ctcgtcgcgc ccccggtaac cgagcaactc ctcgagttcg
     3781 gcgctgcgcc ggccgcacat gcgctcggcg tcactgctcg aataggcggc cagcccgcgc
     3841 gcgatctcgc gcccctcggc atcgcggatc accacggcgt cgccgcgatc gaagcgcccg
     3901 gtgaccgcgg tgacgcccgc gggcagcagg ctcttcccct gctggagcgc gcgcgccgct
     3961 ccgtcgtcga ccttcagttc accggccggc ttcagcatgc cggcgatcca ctgcttgcgg
     4021 gccgccaccg gcgaaccgtc ggccacgaac cacgtgcagc gcgcgcccga ctcgatgcgg
     4081 cgcagcgggt gcggctcacg cccggccgcg acgcacatgt ggcagcccgc agcgaccgcg
     4141 atcttggccg ccaggacctt ggtcgtcatg ccgcccgagc cgacgtccga cgccgacccg
     4201 ccggccatgg cctcgatgtc ggcggtgatg cgcggcactt ccgcgatgaa ccgcgcatcg
     4261 ggctcgcgcg tcggatcggc cgtgtacagg ccgtcgacgt cggacagcag cacgaggcag
     4321 tcggcgctcg ccatctgcgc gacccgcgcc gcgagccgat cgttgtcgcc gtagcggatc
     4381 tcggccgtgg cgacggtgtc gttctcgttg atgaccggga tggccccgag cttgagcagc
     4441 gtcgtcagcg tgttgcgggc gttgaggtag cgccggcgct gctcggtgtc gccgaaagtg
     4501 agcagcacct gtgcgaccgt gcagccgtgg tgctcgagca cctctttcca tgcgtgtgcg
     4561 aggcggatct ggcccacggc cgccgcagcc tggctctgct cgagttccag cttgccggcg
     4621 ggcagcccca gctgccggcg ccccagcgca atcgcgccgg acgacacgag cacgacctcc
     4681 tgcccgcgag cgcgcagccg tgcgatgtcc tcggcgagcg actcgagcca gatgcgattc
     4741 acgcgacccg aggactgctc gaccagcagc gcggagccga ccttgaccac cacccgtcgg
     4801 gccgcggcga ggcgcgagcg gccggccacg gccgacgggg tcatgcgaag ttgaagtgct
     4861 cggcgggcaa gcccatgacg cctgccgcac cggcgcgcgc ggccgccgcg tgcgcgtcga
     4921 cgcgcggcag caaccggtcg aagtagaagc gcgcggtcac gagcttggcc ttgtagaagt
     4981 ccgcgtccgg cgcgccggcc gcgagcttct tcgcggccac gcctgcggag gcgagccagc
     5041 accagcccat gcacacgtag ccggcgaacg ccaggtagtc ggtcgccgcg gcgcccgcct
     5101 cgtccgggtt cttcatcgcg gccatgccga gctcgagcgt gagcttgttc cactgctcga
     5161 gccgcttgcc gagttccgca gccatcgggg ccacctcggg cagcggcgca aacttctcga
     5221 tggtttccct gatgcggccc accatcatct gctggctctt gccctgcgtc ccgaacacct
     5281 tgcggccgag caggtcgagc gcctggatgc cgttcgtccc ctcgtagatc ggcgtgatgc
     5341 gcgcatcgcg catgatctgc tcgacgcccg tctcgcgaat gtagccgtgg cccccgtgca
     5401 cctggatccc gaggctcgcc acctcgaccg cgatgtcggt gaggaacgcc ttcacgatcg
     5461 gcgtgaggaa cgcgacgagg tcgctcgagc ggcggcgcgc gtccgggtcg ggattcatgt
     5521 gcgagccgtc gatctcgcgc ccggtgagga acgcgagcac gcggccaccc tcgacgaatg
     5581 ccttctgcgt gagcagcatc cggcgcacgt cgggatgcac gatgatcggg tcagccggct
     5641 tctccttctg cttgggtccg gtcagcgagc ggccctggat gcgttcgctc gcgtaggcgg
     5701 ccgcggcttg ccaggcgagc tcggagagtc cctggccctg cagtccgacg ccgaggcggg
     5761 cgtggttcat catcgtgaac atgcacgaca ggccttcgtt ctccttgccg agcagccagc
     5821 cgacggactc gtcgaagttc atgacgcagg tcggcgacgc gatgatgccc atcttgtgct
     5881 cgatggagcc gcaggtgacg ccattcgcag gaccgacctt gccgtcggcg tccgggagca
     5941 tcttcggcac cacgaacagg ctgatgccct tggtccccgg cggggcatcc ggcaggcggg
     6001 cgagtacgag gtggacgatg ttgtcggtga ggtcgtgctc gccggcggtg atgaagatct
     6061 tggtgccggt g
//
LOCUS       DAOMFD010000120         5965 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5071_length_5965_cov_41.026058, whole genome shotgun sequence.
ACCESSION   DAOMFD010000120 DAOMFD010000000
VERSION     DAOMFD010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5965)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5965
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5071_length_5965_cov_41.026058"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /locus_tag="PL152_07605"
     CDS             complement(<1..1663)
                     /locus_tag="PL152_07605"
                     /inference="COORDINATES: protein motif:HMM:NF005539.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR49185.1"
                     /translation="MTYFVTGATGFIGRYLVQSLLQRGKGTIYVLVRPKSIGKLDELR
                     EFWGKESVKRVVPIKGDLGSPRLGVSRGDVTKLRRARIRHFFHLGAVYDLEASAEAME
                     KANITGTQGALDLAAAVNAGCFHLVSSIAAAGLYRGTFTEDMFEEAEDLDHPYFRTKH
                     DSEGMVRANGRVPFRIYRPGAVVGHSKTGFIDKIDGPYYFFKALQKLRESWPRWIPLV
                     GLEGGNLNLVPVDYVVDAMVHLAHQKGLDGRCFHLTDPKARRVGEVLNIFARAAHAPE
                     MVMRLDPKILDMVPDNLRKSVERTKPIQNVYEQLLRDLQVPKSVLEFVNHPTRFDSSV
                     TQKLLAKARIHVPPLEDYAWRLWDYWERHLDPDLTIDRSLRGAVRGKVVVITGGSSGI
                     GEAAAIRIAEAGGKVVVVARDEEKLAEVRGKIEAAGGHVKTYSCDITDYAANDKLAKD
                     ILKEYGHVDVLVNNAGRSIRRSLALSYDRFHDFERTIQLNYFACVRLTMNLLPSMTAR
                     KSGHVINVSSIGVLTNAPRFSAYVASKAALESFARCAASEFHDEGV"
     gene            1831..>3260
                     /locus_tag="PL152_07610"
     CDS             1831..>3260
                     /locus_tag="PL152_07610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR49186.1"
                     /translation="MTVTHERMSSVDTAWLRMDGPANSMMIVGVSATETPIRRAQFRR
                     MIQQRLLCFGRFRQRAVADALGASWVTDEDFDLDAHLKYVTLPAPGGKAELQALAADL
                     AGTTLDPARPMWQVHFVDRYLGGSAWITRVHHCYADGIAMIRVLLSMTEQDPAPAMGG
                     APPPQGGKASGKGRPDVLPVLSWIGRFTQPGGDILEHALAEGMKLLEGGVHQVFHPDN
                     VATVASHAGSMIGEFARLLTSADDPDTPLRGQFSGRKAVAWSDPIPLEDVKTVGKALG
                     CTINDVLMSTVAGALGAYLRREGFDTDGLVLRASVPVNLRAADEPLTLGNKFGLVFVD
                     MAVGTRNPLQRLYAMHETMTALKGSMQPPMTLMVLGLMGLLPAALQAPAIDIFSRKGS
                     AVVSNVPGPQAPLQMCGQRISEMYFWVPQSGTIGVGISILSYAGQVFFGMISDRNLVS
                     DPGAVMAQVGPEFEKLLLSVTVGVLAA"
     assembly_gap    3261..3360
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3437..3796
                     /locus_tag="PL152_07615"
     CDS             3437..3796
                     /locus_tag="PL152_07615"
                     /inference="COORDINATES: protein motif:HMM:NF017415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HQR49187.1"
                     /translation="MTAARAKVRDAFSAAQESVQARLDTAREQAEETWDNLEALFQSR
                     VQKAMTRLGIPGAEEFQQLAARVTEMRKELTALGRGRRREGATRRQAAAKAGPKGRAA
                     ASKSRGRARRKSSRARP"
     gene            3800..4591
                     /locus_tag="PL152_07620"
     CDS             3800..4591
                     /locus_tag="PL152_07620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49188.1"
                     /translation="MAGSRDTGAGPKILGVETGGAGRERAPRGELGHLLGRDAELRDV
                     FADDPATHTRLVELQHWQSRRLLTTHADLRANPRYRKAVEFFFRELYGGGDPRGRDRD
                     LARVQRVMERLLPRQALRALCLAIELEVISQELDADVVRALPPGPITVATYAEGYRRA
                     NRADDRQRQIDLLGVIGGYLDGVVRKPWVHALVRLVRGPAHAAGFGVLQDFLERGLDA
                     FETMHGSDEFLAIIRDREVRAMRRVLARTPDPFEFGDGVEPGTAE"
     gene            4603..5259
                     /locus_tag="PL152_07625"
     CDS             4603..5259
                     /locus_tag="PL152_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49189.1"
                     /translation="MSKPRFRRPAIQEELPLPPPPGPPLPVEEVELRWWQQPVAVAAV
                     AALAVLLLILAIVSSVHARRDRALAAELESRVKTLTLRASTAEYALRITPNPRSWSAT
                     PDATLRWPEPPQLLDLRLPVAYSDYTTFAVTVDKVDQGRVMIVQRMTPDSNKELRLSL
                     NSSAFGPGEYRIRIEGYNWRGERSGAGWVRLVVDRPGSSSAPTQPQTSGPNTDTLPAE
                     "
     gene            complement(5211..>5965)
                     /locus_tag="PL152_07630"
     CDS             complement(5211..>5965)
                     /locus_tag="PL152_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HQR49190.1"
                     /translation="AAQSLLPPGGARNALDCALWDLEAKSSGRTIWQLTGVEPREVET
                     VYTISIESTPALMAEHAASAQDCPLLKVKLDGQQPIERMTAIRRTRPDARLVVDANQG
                     WTFDQLQKVAPAFAELGVLMIEQPLPRGGDAALEGYRSPVPLCADESCLHAAELDLAA
                     RRYQMINIKLDKTGGLTHALELARAARARGLGLMVGSMGGTSLAMAPSFVLGCLTDFA
                     DIDGPLLLKRDRLPGMRYSAGKVSVFGPEVWG"
BASE COUNT          897 a         1997 c         2064 g          907 t
ORIGIN      
        1 gcacgccctc gtcgtggaac tcggaggccg cgcagcgcgc gaacgactcg agcgcggcct
       61 tcgaggcgac gtaggcggag aacctcggcg cattggtcag cacgccgatc gaggagacgt
      121 tgatgacgtg cccgctcttg cgcgccgtca tggacggcag caggttcatg gtgaggcgca
      181 cgcacgcgaa gtagttcagc tggatcgtgc gctcgaagtc gtggaagcgg tcgtaggaca
      241 gcgccagcga gcggcggatc gagcggccgg cgttgttcac cagcacgtcg acgtggccgt
      301 attccttgag gatgtccttc gcgagcttgt cgttcgccgc gtagtccgtg atgtcgcagg
      361 aataggtctt cacgtggccg ccagcggcct cgatcttccc gcggacctcc gcaagcttct
      421 cctcgtcgcg cgccacgacg acgaccttgc cgccggcctc ggcgatgcgg atcgcagcgg
      481 cctcgccgat gccggacgat ccgccggtga tgacgaccac cttgccgcgc accgcgccgc
      541 gcaggctgcg gtcgatggtg aggtcggggt cgaggtggcg ctcccagtag tcccacaggc
      601 gccaggcgta atcctcgagc ggcggcacgt gaatgcgcgc cttcgcgagc agcttctgcg
      661 tgacgctcga atcgaagcgc gtcgggtgat tgacgaactc gagcacgctc ttcggcacct
      721 gcaggtcgcg cagcagctgc tcgtacacgt tctggatcgg cttggtgcgc tccaccgact
      781 tgcgcaggtt gtccggcacc atgtcgagga tcttcgggtc gagccgcatc accatttcgg
      841 gggcgtgcgc cgcgcgcgcg aagatgttca ggacctcgcc gacgcggcgt gccttcgggt
      901 ccgtcaggtg gaagcagcgg ccgtcgaggc ccttctggtg cgcgagatgc accatggcgt
      961 cgacgacgta gtccaccggc acgaggttga ggttgccgcc ctcgaggccc acgagcggga
     1021 tccagcgcgg ccaggactcg cgcagcttct gcaacgcctt gaagaagtag taggggccgt
     1081 cgatcttgtc gatgaagccg gtcttcgagt ggccgaccac ggcgcccggg cggtagatcc
     1141 ggaacggcac ccgcccgttc gcccgcacca tgccctcgga gtcgtgcttg gtgcggaaat
     1201 acggatggtc gaggtcctcg gcctcctcga acatgtcctc ggtgaacgtg ccgcggtaca
     1261 ggcccgccgc ggcgatcgag ctcacgagat ggaagcaccc ggcattcacc gccgccgcga
     1321 ggtcgagcgc gccctgcgtg cccgtgatgt tcgccttctc catcgcctcg gcgctggcct
     1381 cgaggtcgta gacggcgccg agatggaaga agtgccggat tcttgcccgg cgcagtttcg
     1441 tgacgtcgcc cctcgacacg ccgaggcgcg gcgagccgag gtcacccttg atcggcacga
     1501 cgcgcttgac gctctccttg ccccagaatt cgcgcagctc gtcgagcttg ccgatggatt
     1561 tcgggcggac cagcacgtag atcgtgccct tgccgcgctg caacagggac tgcaccaggt
     1621 accggccgat gaaaccggtc gcgccggtca cgaagtaggt cattggggtc tcccgactga
     1681 ggcaacgtcg acggaccgcg gtccgccgct ctttcgggct accttaggcg tgggacccgc
     1741 cgaggtcaat gtcacgtacc cggcgccctg gtctggaatt cctgccggga aaatcgtgaa
     1801 aatcggtgcc tggcaccgga gggacaccgc atgacggtca ctcacgagcg catgtcgagc
     1861 gtcgacaccg cgtggctgcg catggacggg ccggccaact cgatgatgat cgtgggcgtg
     1921 tcggccaccg agacgccgat ccgccgcgcg cagttccgcc gcatgatcca gcagcgcctg
     1981 ctgtgcttcg ggcgcttccg gcagcgcgcg gtcgccgacg cgctcggcgc atcgtgggtg
     2041 acggacgagg acttcgacct cgacgcccac ctcaagtacg taacgctgcc cgcgccgggc
     2101 ggcaaggccg aactgcaggc gctggcagcg gatctcgccg gcacgaccct cgatccggcc
     2161 cggccgatgt ggcaggtgca cttcgtggac cgttacctcg gcgggagcgc ctggatcacg
     2221 cgcgtgcatc actgctacgc cgacggcatc gccatgatcc gggtgctgct gtcgatgacc
     2281 gagcaggatc ccgcgccggc gatgggaggc gccccgccgc ctcagggcgg gaaggcgtcc
     2341 ggcaagggtc gaccggacgt cctgccggtg ctgagctgga tcgggcggtt cacgcagccg
     2401 ggcggcgaca tcctcgagca cgcgctcgcc gagggcatga agctcctcga aggcggcgtc
     2461 caccaggtct tccacccgga caacgtggcc acggtcgcga gccacgccgg cagcatgatc
     2521 ggcgagttcg cccggctcct gacgtccgcg gacgacccgg acacgccact gcgcggccag
     2581 ttctcgggac gcaaggctgt cgcgtggtcg gatcccatcc cgctcgagga cgtgaagacc
     2641 gtcggcaagg cgctgggctg cacgatcaac gacgtgctga tgtccacggt cgccggcgcg
     2701 ctcggcgcct acctgcgtcg cgagggcttc gacaccgacg ggctggtgct gcgcgcgtcg
     2761 gtccccgtca acctgcgtgc cgcggacgag ccgctcacgc tcggcaacaa gttcgggctc
     2821 gtgttcgtgg acatggccgt gggcacgcgc aatccgctgc agcggctgta cgccatgcac
     2881 gagacgatga ccgcgctcaa gggctcgatg caaccgccga tgacgctcat ggtgctcggc
     2941 ctgatgggcc tgctgccggc tgcgctgcag gcgcccgcga tcgacatctt cagccgcaag
     3001 ggctcggccg tggtctccaa cgtgccggga ccgcaggcgc cgctgcagat gtgtggccag
     3061 cggatctcgg agatgtactt ctgggttccg cagagcggaa cgatcggcgt cggcatcagc
     3121 atcctgagct acgccggtca ggtgttcttc ggcatgatct cggaccgcaa tctcgtgagc
     3181 gacccgggcg cggtcatggc gcaggtcggg ccggagttcg agaagctgct gctgtcggtg
     3241 accgtcggcg tgctcgcggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3361 tccatctggc tgcggctcac gccgcgagga gagtcacatg cccgcacgcc gcagaaagaa
     3421 tcgaacggag gaatccgtga ctgccgctcg cgccaaggtg cgcgatgcgt tctcggcagc
     3481 gcaggaatcg gtgcaggcgc ggctcgacac cgcgcgtgaa caggccgagg agacctggga
     3541 caacctcgag gccctgttcc agagccgtgt ccagaaggcg atgacgcggc tcgggatccc
     3601 aggcgccgag gaattccagc agctcgcggc gcgcgtcacc gagatgcgca aggagctcac
     3661 cgcgctgggg cgtggacggc ggcgggaagg cgcgacccgg cggcaagcgg cggccaaggc
     3721 cggcccgaag ggccgcgccg cggcgagcaa gtcgcgaggc cgtgcgcgtc gcaagtcgtc
     3781 gcgcgcgcgg ccttaaccat tggcaggctc cagggacacg ggcgccggcc cgaagatcct
     3841 cggcgtcgag accggagggg cgggccgcga acgagcgccg cgcggcgaac tcgggcacct
     3901 gctcgggcgc gacgccgagc tgcgcgacgt cttcgccgac gatccggcca cccacacgcg
     3961 gctggtcgag cttcagcatt ggcaatcgcg gcgcctgctc acgacacacg ccgacctgcg
     4021 cgcgaacccg cgctaccgca aggccgtgga gttcttcttt cgcgaacttt acgggggcgg
     4081 cgacccgcgc ggccgcgacc gcgacctggc gcgcgtgcag cgggtgatgg aacggttgct
     4141 gccgcgccag gcgttgcgtg ccttgtgcct ggcgatcgag ctcgaggtca tttcgcagga
     4201 actcgacgcg gacgtcgttc gcgcgctgcc gccgggcccg atcaccgtcg cgacctacgc
     4261 cgagggttac cggagggcca atcgcgcgga cgaccgccag cggcagatcg acctgctcgg
     4321 cgtcattggg ggctacctcg acggcgtcgt tcgcaaacct tgggtccacg cgctggtcag
     4381 gctggtgcgt ggcccggccc atgcggcggg gttcggcgtg ctgcaggatt tccttgaacg
     4441 gggtctcgat gcgttcgaga ccatgcacgg gtcggatgag tttctcgcta tcatccgcga
     4501 ccgcgaggta cgcgccatgc ggcgcgtcct tgcgcggacg cccgatccgt tcgaattcgg
     4561 cgatggcgtc gagcccggca cggcggagtg agcagcgagc ccatgagcaa accgcgattc
     4621 cgcaggccgg ccatccagga ggagctgcca ctcccgccgc cgcccggccc gccactcccc
     4681 gtggaggaag ttgaactccg ctggtggcaa cagcctgttg cggtcgcggc ggtcgcggcg
     4741 ttggcggtgc tgctgctgat cctcgcgatc gtgtcgagcg tgcatgcaag gcgcgaccgc
     4801 gcgctggcgg cggaactcga gtcccgcgtc aagaccctga cgctgcgcgc gtccaccgcc
     4861 gagtacgcgc tgcgcatcac gcccaatccg cgcagctggt cggccacgcc ggatgcgacg
     4921 ctgcgctggc cggagccgcc acagctgctc gacctgcgcc tgcccgtggc gtactcggat
     4981 tacacgacgt tcgccgtcac cgtcgacaag gtggaccagg gccgcgtgat gatcgtgcaa
     5041 cggatgacgc cagactccaa taaggagtta cggctgtctc tcaacagctc ggcgttcggg
     5101 ccgggcgagt accgcatccg catcgagggc tacaactggc gcggcgagcg gtcgggcgcc
     5161 ggctgggtgc gcctggtcgt cgaccgaccc gggagctcga gcgccccgac tcagccccag
     5221 acctccggac cgaacaccga caccttgccc gccgagtagc gcatcccggg caggcggtcg
     5281 cgcttgagca gcagcggccc gtcgatgtcc gcgaagtccg tgaggcaacc gaggacgaac
     5341 gacggcgcca tcgcaagcga cgtcccgccc atgctgccca ccatcagtcc caggccgcgc
     5401 gcgcgcgccg cgcgcgcgag ttcgagcgcg tgggtcaggc cgccggtctt gtcgagcttg
     5461 atgttgatca tctggtagcg acgcgccgcc agatcgagct ccgctgcatg aaggcacgac
     5521 tcgtcggcgc acagcggaac gggcgaacga tacccttcga gtgcggcgtc gccgccgcgc
     5581 ggcagtggct gctcgatcat gagtacgccg agttccgcga atgcgggtgc gaccttctgc
     5641 agctgatcga atgtccagcc ctggttcgcg tcgacgacca gccgggcatc gggacgggtg
     5701 cggcggatcg cggtcatgcg ctcgatcggc tgctggccgt cgagcttgac cttgagcaac
     5761 gggcagtctt gcgccgacgc cgcgtgctcc gccatgaggg cgggcgtgga ttcgatgctg
     5821 atcgtgtaca ccgtctcgac ctcgcgcggc tcgacaccgg tcagctgcca gatggtgcgc
     5881 ccggacgatt tcgcctcgag gtcccagagc gcgcagtcga gcgcattgcg cgcgccgccc
     5941 ggcggcagca gcgactgcgc cgcgg
//
LOCUS       DAOMFD010000121         5836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5313_length_5836_cov_31.912645, whole genome shotgun sequence.
ACCESSION   DAOMFD010000121 DAOMFD010000000
VERSION     DAOMFD010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5836
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5313_length_5836_cov_31.912645"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..395)
                     /locus_tag="PL152_07635"
     CDS             complement(117..395)
                     /locus_tag="PL152_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49191.1"
                     /translation="MSSRLASLTARRIALQAECARQRADATEAYGEIAAGAERADRLL
                     DVARRLTPVLVVAGVATLVALGPARTFALARRALTIAIYVRQARAVLG"
     gene            complement(392..751)
                     /locus_tag="PL152_07640"
     CDS             complement(392..751)
                     /locus_tag="PL152_07640"
                     /inference="COORDINATES: protein motif:HMM:NF018980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HQR49192.1"
                     /translation="MLRSLVRLVHTALSIAQTRIDLLITEISEDLGRGVRVLLWGVVA
                     VVAGILAALLAGVTAIIYFWDTHRMAASVGVTGVFLLMALGAAGIARARLREKPGLLS
                     ATRSELARDVAALREQP"
     gene            complement(804..1115)
                     /locus_tag="PL152_07645"
     CDS             complement(804..1115)
                     /locus_tag="PL152_07645"
                     /inference="COORDINATES: protein motif:HMM:NF017742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 family protein"
                     /protein_id="HQR49193.1"
                     /translation="MTDAQQVTMEHLRADLAAVMRDAEALLKASADQGGEKVDAARTK
                     VRESMQHAKARLQEAEQAAIRRGQEAKAATEAHIRDNPWQAVGIAAAAGLVIGVLLAR
                     R"
     gene            1478..3421
                     /locus_tag="PL152_07650"
     CDS             1478..3421
                     /locus_tag="PL152_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR49194.1"
                     /translation="MDTKAKDVAMQLAEDARQAEWENVSFTAEVFKGNFRWDLLHPFP
                     AQSAADQQIGDAYIEKIRPVIEKFIDPWKIDEEGEYPREALEAMAEVGLFGMKIPKEY
                     GGLGLSITNYARVLGFLGGYCATTVAYVSAHQSIGVPQPLREFGTEEQKRKYLPRLAK
                     GEISAFALTEPGVGSDPARMATTATPTADGKHYVLNGDKLWCTNVTDPKTTLIAVLAR
                     TPDKVLPDGRKLPQISCFVVETAWPGVERTRRCRFMGLRGLANGIMTLKDVKVPAENL
                     IGKPGEGLKIALATLNVGRLGIPAAAIGGGMALVEDAKWWTTTRQQWGQPVGKHQAVA
                     KLVSGYMAQLFAMKAMVGVACSFADHKSADIRLEAAAAKYWCSEALWKMYDDYVQARG
                     GRGYERATSLYARGDRPTAVEMGLRDARINRIFEGSSQVMHLIMAREALDTHFKLMMP
                     ILQPKKGQADSKGKAAMKAAGFYLGWLPKLYLPGGPRLDAPHLDDRNRAHLAYAARTS
                     KLLARRLFATMAKFGPKLEKEQVILGNFVDIGVDLFVMGATLSYADHLVATNPADKSP
                     LDLADLFCNEARRRIESHFGSVKSNFNRSYDKVSGLLMDGKLDWLAQGAIDPIPPRYR
                     DWQRNLPEAPGTERKAAAPKEAA"
     gene            complement(3477..>5836)
                     /locus_tag="PL152_07655"
     CDS             complement(3477..>5836)
                     /locus_tag="PL152_07655"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQR49195.1"
                     /translation="CLALLAALAAPAHAQVASRLVDQVEVTQAKEYATVTIVFGCEVR
                     YLSHAPASSGEAVRIRLVPGIDCGAPAAGWVVPPPLDDRGVIQSINVVRTVGRDADLT
                     IRWSKGEQFVLVPATNGRGLRILLSRASQKQASVTVREVTGGATAYAVNLDVSQEPYN
                     AAALAAAQQATGVPVYVSETLLDGHKWYRLRAGPFVSESDAKQVLANARARYPKAWLA
                     VADDARLTETGLPDAVGSVQASAAPADVTMSPQDIERTMKRAQEMMRRKDYVVAIQLL
                     TSLTEQPEYPGRAEAVELLGLARERNGQLAHAKAEYEEYLRRYPDGAGAERVSKRLRA
                     ITFESSPAAMQARAAAANQPRWKVYGGVSQTYQRDMTSFDNGTVSRNATTQDAVLTDV
                     ALATRRTGERYDFTARTSGTYGLDLLADGPGNQGTLNLLYAELRDRTLDWTLRGGRQS
                     GSLGGLIGTFDGIYGAYQVLPQMRLDAYYGYPVYSTRASPSTDRNFYAVAADFGTFAE
                     TWDVTVYALQQMYSGLTDREAVGTELRYFRPGLTVIGLVDYDIHFGDVNDALLLVNAA
                     LPADWTLSLNLDHRKSPGLSVGNALMGQSLTSFDQLFDSYSAAQIEQLAVDRTADSDT
                     YTVSLQRMFGERWQWSLDVSGVTIGATPASGGVEATPASGTDMIYSSQAVAYGLFGDR
                     DVDSLGLQYQTGDASDTMSLGLFAQLPVGESWLITPRLRIDRRQFHADGSTQLLYAPG
                     LRTEWRWRRLSLDFEGGAEIGQRSGGIANEDTTRYYFSLGYRYDM"
BASE COUNT          957 a         2007 c         1996 g          876 t
ORIGIN      
        1 tcggcgtcgc cacgatgtgc atcgggctcg gccagggcat cgcgacgatc ctcgaacgct
       61 gcgacggctg aacgctggtc cgggccgccc gaggccgggc gaccgaagcg gcggtttcag
      121 ccaagcacgg cgcgcgcctg acgaacgtag atcgcaatcg tcagcgcgcg tcgcgcgagc
      181 gcgaacgtcc gcgccggacc cagggcaacc agggtcgcga cgcctgcgac gaccagcacc
      241 ggcgtcagcc ggcgcgcgac atcgagcagg cggtccgcac gctccgcgcc cgcagcgatc
      301 tcaccgtagg cttcggtcgc atccgcgcgc tgccgcgcac attcggcctg gagcgcgatc
      361 cgtcgcgccg tgagcgaggc tagccgcgaa ctcacggctg ctccctcaat gcggcgacgt
      421 cgcgcgccag ctcactgcgc gttgcggaga gcaggccggg cttctcgcgc agccgcgccc
      481 gcgcgatccc ggcagcaccg agcgccatga gcaggaacac gcccgtcacg ccgacagaag
      541 ccgccatgcg gtgcgtgtcc cagaagtaga tgatcgccgt gaccccggcg agcagcgcag
      601 ccaggatgcc cgccacgacg gcgaccacgc cccacagcag cacccgcacg ccacgcccaa
      661 gatcctcgct gatctcggtg atcagcaggt cgatgcgcgt ctgcgcgatc gacagtgccg
      721 tgtggaccag gcggaccagc gaccgcagca tgcgcgtcgc tggcccggct gttggctcgc
      781 ccccggtctc gctcatggct ggcctagcga cgcgccagca gcacgccgat caccaggcct
      841 gcagcggcgg cgatcccgac cgcctgccag ggattgtccc ggatgtgggc ttccgtcgct
      901 gccttcgcct cctgtccacg gcggatcgcc gcctgctccg cttcctgcag gcgtgctttc
      961 gcatgctgca tggactcgcg gaccttggtc cgcgcggcgt cgaccttctc tccgccctgg
     1021 tccgcggacg ccttgagcag cgcctcggcg tcgcgcatga cggccgcaag gtcggcccgg
     1081 agatgttcca tcgtcacctg ctgtgcatcg gtcatggctc gtcctcctgc gcaccggcgg
     1141 cccgaagctc tgcacctcgc cgccaacggc ttcatcctag gacgggagac cgtcgcctca
     1201 caatcgtgga agggcgtagc gacgaggtgc ctgcccgatg caccccgctc acccggtggt
     1261 gacgcttcga gcgacggggc gatccttcgc cgacaacagc tggaaccttc caggaagtag
     1321 gggaatcgtc ggcgaaatga cagaacccgg tcaatggcgc tgcaagacac gctgcaccgc
     1381 acaaacaccg ataccgcccg ccgggcaggt gcgggatatt cggcagtcag cggtacacct
     1441 cgttggtcgg gtgggcggaa cagcaggagg aaaggcgatg gacaccaagg cgaaagacgt
     1501 cgcgatgcag ctggccgagg acgcgcggca ggcggaatgg gaaaacgtga gcttcaccgc
     1561 cgaggtgttc aaggggaact tccgctggga cctgctgcac ccgttcccgg cgcagtcagc
     1621 ggcggatcaa cagattggcg atgcatacat cgagaagatc cggccggtca tcgagaagtt
     1681 catcgacccc tggaagatcg acgaagaggg tgaatatcca cgcgaagcgc tggaggccat
     1741 ggccgaggtg ggtctcttcg gcatgaagat cccgaaggag tatggcgggc tgggcctgtc
     1801 gatcaccaat tacgcgaggg tgctgggatt cctcggcggg tactgcgcga cgacggtcgc
     1861 atacgtgtcg gcgcaccaga gcatcggcgt gccgcagccg ctgcgcgagt tcgggacgga
     1921 ggagcagaag cgcaagtacc tgccgcgcct cgcgaagggc gagatctcgg cgttcgcact
     1981 caccgagccc ggcgtcggct ccgacccggc gcgcatggcc accacggcca cgccgaccgc
     2041 ggacggcaag cactacgtgc tgaacggcga caagctgtgg tgcaccaacg tcacggatcc
     2101 gaagaccacg ctgatcgcgg tgctcgcgcg cacgcccgac aaggtgctgc ccgacggtcg
     2161 caagctgccg cagatctcct gcttcgtggt cgagacggcg tggcccggcg tcgagcgcac
     2221 gcggcgctgc cgcttcatgg gcctgcgcgg cctcgccaac ggcatcatga cgctgaagga
     2281 cgtcaaggtc ccggcggaga acctgatcgg gaagccgggc gagggactga agatcgcgct
     2341 cgcgacgctc aatgtcggtc ggctcggcat cccggccgcg gcgatcggcg gtggaatggc
     2401 cctggttgaa gacgcgaagt ggtggaccac cacgcggcag cagtggggcc agccggtcgg
     2461 caagcaccag gcggtggcca agctggtgtc gggttacatg gcgcagctct tcgcgatgaa
     2521 agccatggtg ggcgtggcct gctcgttcgc cgaccacaag agcgcagaca tccgcctcga
     2581 ggcggcggcc gccaagtact ggtgctccga ggcactctgg aagatgtacg acgactacgt
     2641 gcaggcccgc ggagggcgcg ggtacgagcg cgccacgtcg ctgtacgcac gaggtgaccg
     2701 tccgacggcc gtggagatgg gactgcgcga cgcgcgcatc aatcgcatct tcgaggggtc
     2761 atcccaggtc atgcacctga tcatggcccg cgaggcgctc gatacgcact tcaagctgat
     2821 gatgccgatc ctgcagccga agaagggcca ggcggattcg aagggcaagg cggccatgaa
     2881 ggcggccggc ttctacctgg gctggctgcc gaagctgtac ctgccgggcg gcccgcgtct
     2941 cgacgctccg cacctggacg accggaaccg cgcgcatctc gcgtacgccg ccaggacttc
     3001 gaagctcctc gcccgccggc tgttcgcgac catggcgaag ttcgggccga agctcgagaa
     3061 ggagcaggtc atcctcggca atttcgtgga catcggcgtc gacctgttcg tgatgggcgc
     3121 gacgctctcg tacgcggacc acctcgttgc gaccaaccct gccgacaagt ccccgctcga
     3181 cctcgcggac ctcttctgca acgaggcgcg tcgtcgcatc gagagccatt tcgggagcgt
     3241 gaagagcaac ttcaaccgct cttacgacaa ggtgtccggc ctgctgatgg atgggaagct
     3301 ggactggctg gcgcagggtg cgatcgaccc gatcccgcct cgctatcgcg actggcagag
     3361 gaatctgccc gaagcgccgg gcaccgagcg caaggcggcc gcgccgaagg aagcggccta
     3421 gaaaatcggg gacattctcc tatttcctga ggaaattagg agaatgtccc cattttctac
     3481 atgtcgtagc gatagccgag gctgaagtag tagcgcgtcg tgtcttcgtt cgcgatgccg
     3541 ccgctgcgct gcccgatttc ggcgccgccc tcgaagtcca gcgacagcct gcgccagcgc
     3601 cactccgtgc gcagaccggg cgcgtagagc agctgcgtcg agccatcggc gtggaactgc
     3661 cgccgatcga tgcgcaggcg cggcgtgatg agccacgact cgcccacggg cagctgcgcg
     3721 aacaggccca gcgacatcgt gtcggacgcg tctccggtct ggtactgcag ccccagcgaa
     3781 tcgacgtcac ggtcgccgaa caggccgtac gccaccgcct gtgacgaata gatcatgtcc
     3841 gtgccggacg ccggcgtcgc ctcgactccg cccgaggccg gtgtcgcccc gatggtgacg
     3901 ccggacacgt ccagcgacca ctgccaacgc tcaccgaaca tgcgctgcag cgacacggtg
     3961 taggtgtcgg aatccgccgt cctgtcgacc gccagctgct cgatctgggc agccgagtac
     4021 gagtcgaaca gctggtcgaa cgaggtgagc gactgcccca tgagcgcgtt gcctacgctc
     4081 aagcccgggc tcttgcgatg gtcgaggttg aggctcagcg tccagtccgc gggcagcgcc
     4141 gcattgacga gcagcagcgc gtcgttcacg tcgccgaagt gtatgtcgta gtcgacgagc
     4201 ccgatcaccg tgaggccggg ccggaagtag cgcagttccg tgccgacggc ctcgcggtcg
     4261 gtgagcccgg agtacatctg ctgcagcgcg tagaccgtga cgtcccaggt ctccgcgaac
     4321 gtgccgaagt cggcggccac cgcgtagaag ttgcggtccg tcgacggcga cgcgcgcgtc
     4381 gaatacaccg ggtagccgta gtaggcatcg agtcgcatct gcggcaggac ctggtaggcg
     4441 ccgtagatgc cgtcgaacgt gccgatcagc ccgccgaggc tgcccgactg ccggccaccg
     4501 cgcagcgtcc agtcgagcgt ccggtcgcgc agttcggcgt acagcaggtt caacgtaccc
     4561 tggttgcccg gcccgtcggc cagcaggtcg aggccgtagg tgccgctcgt tcgggccgtg
     4621 aagtcgtagc gctccccggt gcgccgcgtc gccagcgcga cgtcggtcag caccgcgtcc
     4681 tgcgtggtcg cgttcctcga gaccgtaccg ttgtcgaagc tggtcatgtc acgctggtag
     4741 gtctgcgaca cgccgccgta gaccttccag cgcggctggt tcgcggccgc cgcgcgcgcc
     4801 tgcatggcgg ctgggctcga ctcgaaggtg atcgcgcgca gccgcttgct gacccgctcc
     4861 gcgcccgcgc cgtcgggata gcgacgcagg tactcctcgt actcggcctt cgcgtgggcg
     4921 agctgcccgt tgcgctcgcg agccaggcca agcagttcca cggcttcggc gcgccccggg
     4981 tactcgggct gctcggtgag gctcgtgagc aactggatcg cgacgacgta gtccttgcgg
     5041 cgcatcatct cctgcgcgcg cttcatggtt cgctcgatgt cctgcggcga catcgtgacg
     5101 tcggccggcg ccgccgacgc ctgcacgctg ccgaccgcgt ccggcaggcc ggtctcggtc
     5161 aggcgcgcat cgtcggctac ggccagccat gccttcgggt accgggcacg ggcgttggcc
     5221 agcacctgct tcgcgtccga ttccgacacg aacggccccg cgcgcagccg gtaccacttg
     5281 tgaccgtcga gcagcgtctc ggacacgtag accgggactc cggtcgcctg ctgcgccgct
     5341 gcgagcgccg ccgcgttgta gggctcctgg gagacgtcga ggttgaccgc gtaggccgtg
     5401 gcgccaccgg tgacctcgcg caccgtcacc gacgcctgct tctgcgacgc gcgcgacagc
     5461 aggatgcgca gcccgcgccc gttggttgcg ggcaccagca cgaactgctc gcccttcgac
     5521 cagcgaatcg tgaggtcggc gtcccgaccc accgtgcgca cgacgttgat cgactggatc
     5581 acgccgcggt cgtcgagcgg gggagggacg acccagccag cggccggcgc gccgcagtcg
     5641 atgccgggaa cgaggcggat gcgcaccgcc tcgccgctgc tcgccggcgc gtggctcagg
     5701 taccgaacct cgcagccgaa cacgatggtg acggtggcgt actccttcgc ctgggtcact
     5761 tcgacctggt cgaccagccg cgacgcgacc tgcgcgtgcg caggagcggc gagcgctgcg
     5821 agcagcgcca ggcacg
//
LOCUS       DAOMFD010000122         5819 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5342_length_5819_cov_32.900243, whole genome shotgun sequence.
ACCESSION   DAOMFD010000122 DAOMFD010000000
VERSION     DAOMFD010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5342_length_5819_cov_32.900243"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1113
                     /locus_tag="PL152_07660"
     CDS             <1..1113
                     /locus_tag="PL152_07660"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HQR49196.1"
                     /translation="ASELQRFGTPKATAADIFAALSAVYRRIRGGYAVVAMILGHGVV
                     GFRDPNGIRPLVIGTREGVRGREYMLASESVALDQGGFKLLRDVGPGEAVYIEEQGRM
                     HTHQCIEAPRHTPCIFEYVYFARPDSIIDNISVYKARMRMGELLADKIRRERPDHDID
                     VVIPVPDTSRTSALQVAQLLGVKYREGFIKNRYIGRTFIMPGQEQRAKSVRSKLNPID
                     LEFRGRNVLLVDDSIVRGTTSAQIIEVAREAGARRVYFASAAPPVRHPNVYGIDMPAA
                     SELVAAGRTEQEVARIIGTDWLIYQDLDDLVRAVRHHDARVTEFDTSCFSGEYVTGDV
                     TPEYLARLQAERSDAAKSQRNASGGGRGPLRVVGGP"
     gene            1130..2368
                     /locus_tag="PL152_07665"
     CDS             1130..2368
                     /locus_tag="PL152_07665"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HQR49197.1"
                     /translation="MTVKALIVSDRSDYRQLLAHHLTLEWRDALPAEYEPATRGCLQS
                     AFRGAAYDVVLLDHEVQGGRGLAWLEDLCARAGFPPIVYFAPTGDASALAAGATQVLA
                     RLEFDHEQFASALRAVLAQRHNVLAHASRAALEPPPPDRFGSVRIRGFRYVRRIAVGG
                     SSSVFLAELERTGEPRVLKVFRQVPDVVEGGTTFDRFLREFDVAARLDHPNIARIYDI
                     GVADDHVYLTMEYFPGGDLRSRMQQPLPWRDALGYLRQLAAALGALHEVGVLHRDVKP
                     GNVLVRGDGSIAFIDFGLARQLGLESDITGNGAIFGTPHYMSPEQGHGLPLDERSDLY
                     SLGVMLYEMLTGEKPFPAETPLAVIYKHANAPVPTLPAACAHLQPLLDELLAKDASSR
                     PATAAEIVSRIDELLDRAAA"
     gene            2368..>2879
                     /gene="miaE"
                     /locus_tag="PL152_07670"
     CDS             2368..>2879
                     /gene="miaE"
                     /locus_tag="PL152_07670"
                     /inference="COORDINATES: protein motif:HMM:NF017939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045301 -
                     tRNA-(2-methylthio-N-6-(cis-hydroxy)isopentenyl
                     adenosine)-hydroxylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA isopentenyl-2-thiomethyl-A-37 hydroxylase
                     MiaE"
                     /protein_id="HQR49198.1"
                     /translation="MRGARDILRASTDAAWFDAAAAREDELLIDHANCEKKAASTALS
                     LMFAYPEDFELAARMSRLAREELRHFELVQEKLTELGVAFRRLSPSRYAEGLRRALRR
                     SEPGRRVDLLLCGALIEARSHERFLGLVARLRKPLADFYESLAAAESRHAGLYLRLAE
                     QRGTEVGSRL"
     assembly_gap    2880..2979
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3080..3805)
                     /locus_tag="PL152_07675"
     CDS             complement(3080..3805)
                     /locus_tag="PL152_07675"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF003743.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HQR49199.1"
                     /translation="MTTLFISDLHLDPGEPAITRQLLRLLDGEARRADALYILGDLFE
                     VWLGDDDPDPAAREVVTALRRLTDAGVPCYVMHGNRDFLIGRRFCRETGAVLLDDSTV
                     VDLHGERVLLMHGDTLCTDDTSYQRLRRIVRNPVVKWVFRRMSLDRRRALAQKMREGS
                     RMHVGSTAPEIMDVNRDAVADAFRRTGVRTLIHGHTHRPAVHELVVDGSPVRRIVLGD
                     WHTQGSVLAWSADDHFELRSLPR"
     gene            3789..5414
                     /gene="gltX"
                     /locus_tag="PL152_07680"
     CDS             3789..5414
                     /gene="gltX"
                     /locus_tag="PL152_07680"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HQR49200.1"
                     /translation="MKRVVMRNGALALPDGTVPGLVQASRMRPRLARIGGGSQGAAAA
                     VEDWWTLVAEGGLLDPVRCAPMSNAPHVTRFAPSPTGSLHLGNARTALFNHLAARASG
                     GRMILRVEDTDAERSDESHLQGLLQDLRWLGVEWDEGPDVGGPRGPYRQSERGQRYAA
                     ALSLLRARGLAYPCFCSPEELAISRRAQLAAGRPPRYAGTCAGLPAEEAERRIAEGRA
                     YATRFRVPPGRTVEFEDVIHGHQRFASDDIGDFVVSRADGSVAFFLGNAVDDADMGVT
                     LVLRGDDHLANTPRQILLLEALGLPVPRYGHLPLVLGPTGAPLSKREGAASLHDLREQ
                     GYLPGALTNYLVRLGHACAGDEWLETAQMPSHFDLARSSRSPARFDEAQLRHWQREAV
                     THATIAQLAGWLGSRLGELGDAARKGVFVEAVRGNLLFPVDVEPLVAIVCREEVPVEA
                     EAATAVVEAGPGFFDQAVAVWATSAGDFKAWARAVGESTGRKGARLYQPLRAALTGLT
                     HGPELAPLAALMGRERVAARLAAAGRLAAARAG"
     gene            5440..>5819
                     /locus_tag="PL152_07685"
     CDS             5440..>5819
                     /locus_tag="PL152_07685"
                     /inference="COORDINATES: protein motif:HMM:NF013565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HQR49201.1"
                     /translation="MLQIHNSLTGRKEAFQPIVPGEVRMYVCGMTVYDYCHLGHARMM
                     IAFDVVRRHLVASGYRVNFVRNITDIDDKIIKRAAENGEPMGALTERFIRAYHEDCDA
                     LGVARADHEPRATEYLPQIVSMVRT"
BASE COUNT          817 a         1980 c         2064 g          858 t
ORIGIN      
        1 gcgagcgagc tgcagcgctt cggcacgccc aaggccacgg cggccgacat cttcgccgcg
       61 ctgtcggcgg tgtaccgccg catccgtggc ggatacgcgg tcgtggccat gatcctggga
      121 cacggagtgg tgggcttccg cgacccgaac gggatccgtc cgctggtcat cggcacgcgt
      181 gaaggcgtcc gcgggcgcga gtacatgctc gcttccgaga gcgtcgcgct cgaccagggc
      241 ggcttcaagc tgctgcgcga cgtcggtccc ggcgaagccg tgtacatcga ggagcagggt
      301 cgcatgcaca cgcaccagtg catcgaggcg ccgcgccaca cgccctgcat cttcgagtac
      361 gtctatttcg cgcggcccga ctcgatcatc gacaacattt ccgtgtacaa ggcccgcatg
      421 cgcatgggcg agctgctggc ggacaagatc cgccgcgagc ggccggacca cgacatcgac
      481 gtcgtgatcc cggtgcccga caccagccgc accagtgcgc tgcaggtggc gcagctgctg
      541 ggggtcaagt accgcgaggg cttcatcaag aaccgctaca tcggccgcac cttcatcatg
      601 ccgggccagg agcagcgggc gaagtcggtg cgcagcaagc tgaacccgat cgacctcgag
      661 ttccgcggcc gcaacgtgct gctggtggat gactcgatcg tgcgcggcac gacctccgcg
      721 cagatcatcg aggtcgcgcg cgaggccggc gcgaggcgcg tgtatttcgc ctcggccgcc
      781 ccgccggtgc gtcatccgaa cgtgtacggc atcgacatgc cggcggcgtc cgagctggtg
      841 gcggcgggcc gcaccgagca ggaggtcgcg cgcatcatcg gcaccgactg gctcatctac
      901 caggacctcg acgacctggt gcgtgccgtg cgtcaccacg acgcgcgtgt caccgagttc
      961 gacacgtcgt gtttctccgg cgaatacgtc acgggcgacg tgactcccga gtacctcgcg
     1021 cggctgcagg cggagcgttc cgacgcggcc aagtcgcagc gcaacgcctc cggcggcggg
     1081 cgggggccgc tcagggtcgt cggcgggccc tgaaccgccg caccgccgga tgacggtcaa
     1141 ggcgctcatc gtcagcgacc ggtcagacta ccggcagctg ctcgcccacc acctgacgct
     1201 cgagtggcgc gacgcgctgc ccgccgagta cgagccggcg acgcgcggct gcctgcagtc
     1261 ggcgttccgc ggcgcggcgt acgacgtggt gctgctcgat cacgaggtgc agggcggacg
     1321 cggtctcgcg tggctcgagg acctctgcgc gcgcgccggg ttccctccga tcgtgtactt
     1381 cgcgccgacc ggcgacgcca gcgcgctcgc cgccggcgcc acgcaggttc ttgcccggct
     1441 cgagttcgat cacgagcagt tcgcgtcggc attgcgcgcc gtgctcgccc agcggcacaa
     1501 cgtcctggcg cacgcgtcgc gcgcggcgct ggagcctccg ccgccggacc gcttcgggtc
     1561 ggtgcgcatc cgggggttcc gctatgtccg acgcatcgcc gtcggtggct cgtcgagcgt
     1621 attcctcgcc gagctggagc gcaccggcga accgcgggtg ctgaaggtgt tccgccaggt
     1681 gcccgacgtg gtcgagggcg gcaccacctt cgatcgcttc ctgcgcgagt tcgacgtcgc
     1741 cgcgcggctc gatcacccga acatcgcgcg catctacgac atcggggtgg cggacgacca
     1801 cgtgtacctg acgatggaat acttccccgg cggggacctg cgctcgcgca tgcagcagcc
     1861 gctgccgtgg cgcgacgcgc tcggctacct gcgccagctc gcggcggcgc tcggcgcgct
     1921 gcacgaggtg ggcgtgctgc accgggacgt caagcccggc aacgtgctgg tgcgcggcga
     1981 cggcagcatc gcattcatcg acttcggcct cgcgcggcag ctcgggctgg agagcgacat
     2041 caccggcaac ggggcgatct tcggcacgcc gcactacatg agcccggagc agggccacgg
     2101 cctcccgctc gacgagcgca gcgacctcta cagtctcggc gtcatgctgt acgagatgct
     2161 gaccggcgag aagccgttcc cggccgagac gccgctcgcg gtcatctaca agcacgccaa
     2221 tgcgccggtg ccgaccctgc cggccgcctg cgcgcacctg caaccgctgc tcgacgagct
     2281 gctcgcgaag gatgcgtcga gtcgccccgc gacggccgcg gagatcgtct cccgcatcga
     2341 cgagttgctc gaccgcgcgg cggcctgatg cggggcgcgc gcgacatcct gcgcgcgtcg
     2401 accgacgccg cgtggttcga tgcggcggcc gcgcgcgagg acgagctgct catcgaccac
     2461 gccaactgcg agaagaaggc ggcgtcgacc gcgctgtcgc tcatgttcgc gtatccggag
     2521 gacttcgagc tggcggcccg catgtccagg ctggcgcgcg aggaactgcg ccacttcgag
     2581 ctggtgcagg agaagctgac cgagctgggc gtggcgttcc gtcgcctctc gccgtcgcgc
     2641 tatgccgagg gcctgcgccg tgcgctgcgc aggtcggagc cggggcgccg cgtcgacctg
     2701 ctgctgtgcg gggcgctcat cgaggcacgg tcccacgagc ggttcctggg cctggtcgca
     2761 cggctgcgca agccgctcgc cgatttctac gagtcgctgg ccgccgctga atcacgccac
     2821 gccgggctct acctgcggct cgccgagcag cgcggcacgg aggtggggtc gcgcctcgan
     2881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng ggcggggacg ccgtgaaccc
     3001 atccatgggg gctcgcacgc ggcgtccatg ccgcgtgacg ccccgccctc gaatgtccca
     3061 gtcccgacgg ccgccctcgc tagcgcggca ggctgcgcag ttcgaagtgg tcgtcggcgg
     3121 accacgcgag cacgctgcct tgcgtgtgcc agtcgccgag cacgatgcgc cgcaccggag
     3181 agccgtccac gacgagttcg tgcacggccg gccgatgcgt atggccgtgg atcaaggtgc
     3241 gtacgccggt ccggcggaag gcatcggcca ccgcgtcccg attcacgtcc atgatctccg
     3301 gcgcggtcga tccgacgtgc atgcggctgc cttcgcgcat cttctgcgcg agggcgcgcc
     3361 gccggtcgag gctcatgcgg cggaacaccc acttgacgac cgggttgcgc acgatgcgcc
     3421 gcagccgctg gtagctcgtg tcgtcggtgc aaagcgtgtc gccgtgcatc agcagtacgc
     3481 gctcgccgtg gagatcgacc acggtgctgt cgtcgagcag cacggccccg gtctccctgc
     3541 agaagcgcct gccgatgagg aagtcgcggt tgccgtgcat gacgtagcag ggcacgcccg
     3601 cgtcggtgag ccggcgcagc gccgtgacga cctcgcgggc tgcggggtcc gggtcgtcgt
     3661 cgccgagcca gacttcgaac aggtcgccga ggatgtagag cgcgtcggcg cggcgggcct
     3721 cgccatccag caagcggagc agctgccggg tgatcgcggg ctcgccgggg tcgaggtgca
     3781 ggtcggaaat gaagagagtc gtcatgcgga acggggccct ggcgttgccg gatggtacgg
     3841 tgcctggcct cgtgcaggcc agccggatgc ggccgcggct tgcgaggatc ggcggtggct
     3901 cgcaaggcgc ggcggcggct gtcgaggact ggtggaccct cgtcgccgag ggcgggctgc
     3961 tcgatccggt aagatgcgcg cccatgtcga atgcgcctca cgtgacgcga ttcgcgccga
     4021 gcccgaccgg aagtctccac ctcgggaatg cgcgcaccgc gctgttcaac cacctcgctg
     4081 cacgggcgag cggtgggcgc atgatcctgc gcgtcgagga caccgacgcc gaacgcagcg
     4141 acgagtccca cctgcagggc ctgctgcagg acctgcgctg gctcggtgtc gagtgggacg
     4201 aggggccgga cgtcggcggc ccgcgcggcc cgtaccgcca gagcgagcgc ggccagcgct
     4261 acgcggcagc gctgtcgctc ttgcgcgcgc gtggcctcgc gtatccctgc ttctgctcgc
     4321 cggaggagct cgcgatctcg cggcgcgcgc agctcgccgc aggccgtccg ccgcggtacg
     4381 cggggacctg cgccggattg ccggcggagg aggccgaacg gcgcattgcg gagggcaggg
     4441 catacgccac ccgttttcgt gtcccgcccg gccgcacggt tgagttcgag gacgtcatcc
     4501 acggacacca gagattcgcg agcgacgaca tcggcgactt cgtcgtgagt cgcgcggacg
     4561 gttcggtggc tttcttcctg ggcaatgccg tggacgatgc cgacatgggc gtgacactgg
     4621 tgctgcgcgg cgatgaccac ctggcgaata caccgcggca gatcctgctg ctcgaggcgc
     4681 tgggactgcc ggtgccgcgc tacggccacc ttccgctggt gctggggccg accggtgcgc
     4741 cgctgtccaa gcgcgagggt gcggccagcc tgcacgacct gcgtgaacag ggatacctgc
     4801 cgggagcgct cacgaactac ctggtgcggc tggggcatgc gtgcgccggc gacgaatggc
     4861 tggagactgc gcagatgcct tcccatttcg acctcgcgcg cagcagccgt tcgccggcgc
     4921 gattcgacga agcgcagttg cgtcactggc agagggaggc cgtcacgcac gcgacgatcg
     4981 cgcagctggc cggctggctc ggatcgaggc tcggcgaact cggcgacgcg gcgcgcaagg
     5041 gcgtcttcgt cgaggccgtg cgcggcaacc tgctgttccc ggtggacgtc gagccgctcg
     5101 tggcgatcgt ctgccgcgag gaagtacccg tcgaggccga ggccgcaact gcggtcgtgg
     5161 aggcgggccc cggctttttc gatcaggctg tcgccgtgtg ggcaacgagc gcgggtgatt
     5221 tcaaggcgtg ggctcgggcc gtcggcgaat cgacgggtcg caagggtgcg cgactgtacc
     5281 agccgctgcg tgcggcgctg accggcctga cgcacgggcc ggagcttgcg ccgctcgctg
     5341 cattgatggg ccgggagcgt gtggcggccc ggctcgccgc ggccggtcga ctcgccgctg
     5401 cgcgcgcagg atgagacggg attccggaga cgttcgacga tgctgcagat ccacaactcg
     5461 ctgaccggcc gcaaggaagc gttccagccc atcgtgccgg gcgaggtccg catgtacgtc
     5521 tgcggcatga ccgtgtacga ctactgccac ctcggtcacg cacgcatgat gatcgcgttc
     5581 gacgtcgtgc gacggcacct cgtcgcttcc ggttatcgcg tgaacttcgt ccgcaacatc
     5641 accgacatcg acgacaagat catcaagcgg gcggcggaga acggcgagcc gatgggcgcc
     5701 ctgaccgagc gtttcatccg cgcctatcac gaggactgcg acgcgctcgg cgtcgctcgc
     5761 gccgatcacg agccgcgcgc gaccgagtac ctgccgcaga tcgtctcgat ggtgcgcac
//
LOCUS       DAOMFD010000123         5780 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5400_length_5780_cov_34.616943, whole genome shotgun sequence.
ACCESSION   DAOMFD010000123 DAOMFD010000000
VERSION     DAOMFD010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5780)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5780
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5400_length_5780_cov_34.616943"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1889
                     /gene="dnaK"
                     /locus_tag="PL152_07690"
     CDS             <1..1889
                     /gene="dnaK"
                     /locus_tag="PL152_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HQR49202.1"
                     /translation="VAIMDGGKPRVIENSEGDRTTPSIVAFTKDDEVLVGQSAKRQAV
                     TNPQNTLYAIKRLIGRRYDDAVVQKDVGMVPYKISKADNGDAWIEVHGKKMAPPEISA
                     RVLMKMKKTAEDFLGEKVTEAVITVPAYFNDSQRQATKDAGRIAGLEVKRIINEPTAA
                     ALAYGLDKAGGDRKIAVYDLGGGTFDISIIELADVDGERQFEVLSTNGDTFLGGEDFD
                     KRVIDYLAAEFQKESGVDVRRDPLAMQRLKEAAEKAKIELSSSQQTEVNLPYITADAS
                     GPKHLAMKLTRAKLESLVEDLVQKTIEPCRIALKDAGLSINEVAEVILVGGQTRMPLV
                     QKFVKDFFGKEPRKDVNPDEAVAVGAAVQAGVLTGEVKDVLLLDVTPLSLGIETLGGV
                     MTKLIEKNTTIPTKATQVFSTADDNQTAVTIHVLQGERDRASDNKSLGRFDLSDIPPA
                     PRGMPQVEVTFDIDANGILNVSAKDKATGKEQKIVIKASSGLNEDEIKRMVRDAEAHA
                     EEDRKFRELAEARNRADALLHATDKALKDLGDKVSAADRAKVESAISDLKGVIKGEDK
                     DVIETKARALAEASATIAQQAYAGAQQGPGGAGAAGGGQPGGKEEVLDAEFEEVKDKD
                     AKGN"
     gene            1987..3120
                     /gene="dnaJ"
                     /locus_tag="PL152_07695"
     CDS             1987..3120
                     /gene="dnaJ"
                     /locus_tag="PL152_07695"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQR49203.1"
                     /translation="MSKRDYYQVLDVPRTASEADVKKAYRRLAMKFHPDRNPDDHEAT
                     DKFKEVKEAYEVLSDAQKRAAYDQFGHAGVDGTRGAGGPGFDPRDAFGDIFGDVFGDI
                     FGVGRRGRSQVYRGADLRYELELTLEQAVFGANVNIDFATLAECAECGGSGSSKGSKP
                     VTCDTCRGAGQVRMQQGFFTVQQTCPRCNGRGQVITDPCGSCRGQGRVRKQKTLSVKV
                     PQGVDTADRIRLSGEGEAGRNGGPPGDLYVEIRVKDHPIFERDGSHLSCEVPVSFATA
                     ALGGSIEVPTLGGNATIKIPAETQSGRVFRLREKGIRPVRGGAVGDLFCRIVVETPVN
                     LSRDQKDLLRKFEDSLQKDAKHHHPREESWLDGVKRFFSSLGD"
     gene            3125..3925
                     /gene="dapB"
                     /locus_tag="PL152_07700"
     CDS             3125..3925
                     /gene="dapB"
                     /locus_tag="PL152_07700"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HQR49204.1"
                     /translation="MLNVAMLGSSGRMGRTIVPLVAEAGDLQLSGALCAADDPAVGRD
                     AGVLAGVAPLAVAISDDPAIALAGAQVAIDFTLPAVSVANARLCRERGCPLVIGTTGH
                     SAAQRAELEAVAETLPVVLAPNMSLGVNLLFKLAELAARALDEQYDIEIFEAHHRNKV
                     DAPSGTALGLGRAAAAGRGTTLEKAGEYARHGQTGIRERGKIGFSVVRGGDIVGEHRL
                     IFAGPGEQVELAHRAQDRSGFARGALVAARWVVGRPPGLYSMLDVLGL"
     assembly_gap    3926..4025
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4192..5355
                     /gene="carA"
                     /locus_tag="PL152_07705"
     CDS             4192..5355
                     /gene="carA"
                     /locus_tag="PL152_07705"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005951 - carbamoyl-phosphate synthase
                     complex [Evidence IEA];
                     GO_function: GO:0004088 - carbamoyl-phosphate synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HQR49205.1"
                     /translation="MVVPTPAVLALEDGTVFRGISIGAAGTATGEVVFNTAMTGYQEI
                     LTDPSYARQIVTLTYPHIGNTGTNPDDLESGSVHSAGLVIRNLPLLHSNWRATETLPE
                     FLTRGGIVAIAEVDTRRLTRILREKGAQAGCIASGDAASRPDLAIEAARRFPGLKGMD
                     LAKVVSTKRSFQWNEGTNWALEQGPRRRPGQRLHVVAYDFGIKRNILRLLADHGCRMT
                     VVPAATTAAEVLALGPDGVFLSNGPGDPEPCTYAIDAIKALVAAAVPTFGICLGHQLL
                     GLAMGARTVKMKFGHHGANHPVIELDSGRVFISSQNHGFAVDEATLPPNVRATHRSLF
                     DGSLQGIEVTDRPAFSFQGHPEASPGPRDLAPLFARFVEAILRRGMIPVRKPA"
     gene            5375..>5780
                     /gene="carB"
                     /locus_tag="PL152_07710"
     CDS             5375..>5780
                     /gene="carB"
                     /locus_tag="PL152_07710"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648321.1"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl phosphate synthase large subunit"
                     /protein_id="HQR49206.1"
                     /translation="MPKRTDIQSVLIIGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPETADSIYIEPINWRTIARIIEKERPDALLPTMGGQTALNCALDL
                     VREGVLEQFGVELIAASREAIDMAEDRELFRNA"
BASE COUNT          995 a         1928 c         1985 g          772 t
ORIGIN      
        1 gcgtcgccat catggatggc ggcaagcccc gcgtgatcga gaacagcgag ggtgaccgca
       61 cgaccccgtc catcgtcgcc ttcaccaagg acgacgaggt gctggtcggg cagtcggcga
      121 agcgccaggc ggtcaccaac ccgcagaaca ccctgtacgc gatcaagcgc ctgatcggcc
      181 gccgctacga cgacgcggtc gtgcagaagg acgtgggcat ggtgccctac aagatcagca
      241 aggccgacaa cggcgacgcg tggatcgagg tgcacggcaa gaagatggcg ccgcccgaga
      301 tctcggcgcg cgtcctcatg aagatgaaga agacggccga ggacttcctc ggcgagaagg
      361 tgacggaagc cgtcatcacg gtgcccgcct acttcaacga ctcgcagcgc caggcgacca
      421 aggacgccgg ccgcatcgcc ggcctcgagg tcaagcgcat catcaacgag cccaccgccg
      481 cagcgctcgc ctacggtctc gacaaggccg gcggcgaccg caagatcgcc gtgtacgacc
      541 tcggcggcgg cacgttcgac atctccatca tcgagctcgc ggacgtggac ggcgagcgcc
      601 agttcgaggt gctctcgacc aacggcgaca cgttcctcgg cggcgaggac ttcgacaagc
      661 gcgtgatcga ctacctggcg gccgagttcc agaaggagtc gggcgtggac gtgcggcgcg
      721 acccgctcgc catgcagcgc ctgaaggaag cggccgagaa ggccaagatc gagctgtcgt
      781 cgagccagca gaccgaggtc aacctgccgt acatcacggc cgacgcctcc ggcccgaagc
      841 acctggcgat gaagctcacc cgcgccaagc tcgagtcgct ggtcgaggac ctcgtgcaga
      901 agacgatcga gccttgccgc atcgcgctca aggacgccgg cctgtccatc aacgaagtgg
      961 cggaggtcat cctcgtcggc ggccagaccc gcatgccgct ggtgcagaag ttcgtgaagg
     1021 acttcttcgg caaggagccg cgcaaggacg tgaacccgga cgaagccgtt gccgtcgggg
     1081 cagcggtgca ggccggcgtg ctcacgggtg aagtgaagga cgtgctgctc ctcgacgtca
     1141 ccccgctgtc gctcggcatc gagacgctcg gcggcgtgat gaccaagctg atcgagaaga
     1201 acaccacgat cccgaccaag gcgacgcagg tgttctcgac cgccgacgac aaccagacgg
     1261 cggtgacgat ccacgtcctg cagggcgagc gcgaccgcgc cagcgacaac aagtcgctcg
     1321 gccggttcga cctctcggac atcccgccgg cgccgcgcgg catgccgcag gtcgaggtca
     1381 cgttcgacat cgacgcgaac ggcatcctca acgtgtcggc caaggacaag gccacgggca
     1441 aggagcagaa gatcgtcatc aaggcctcga gcggcctgaa cgaggacgag atcaagcgca
     1501 tggtgcgcga cgcggaggcg cacgccgagg aggaccgcaa gttccgcgag ctcgccgagg
     1561 cgcgcaatcg cgccgacgcg ctgctccacg cgaccgacaa ggcgttgaag gacctcggcg
     1621 acaaggtctc ggccgccgat cgcgccaagg tcgagtcggc gatctccgac ctcaagggcg
     1681 tgatcaaggg cgaggacaag gacgtgatcg agaccaaggc ccgcgcgctc gcggaagcct
     1741 cggcgacgat cgcccagcag gcctacgccg gcgcgcagca gggccctggc ggcgcgggtg
     1801 ccgcgggcgg cggccagccg ggcggcaagg aagaggtgct cgacgccgag ttcgaggaag
     1861 tgaaggacaa ggacgccaag ggcaactgat cctccccgag gcgctatcct tgtccaggtg
     1921 atccaacccg ccgcggggga tgcgtccctc gcggcggttc cacattgaag accgctcaag
     1981 gcccgaatgt cgaagcgcga ttactaccag gtcctcgacg tgccgcgcac cgcgagcgag
     2041 gcggacgtca agaaggccta tcgccggctg gcgatgaaat tccatccgga ccgcaacccc
     2101 gacgaccacg aggcgaccga caagttcaag gaggtcaagg aggcctacga ggtcctgtcg
     2161 gatgcgcaga agcgagccgc ctacgaccag ttcggccacg ccggggtgga cggaacccgc
     2221 ggcgccggcg gcccgggatt cgacccgcgc gacgcgttcg gcgacatctt cggcgacgtc
     2281 ttcggcgaca tcttcggggt cggccgccgc ggccgctcgc aggtgtaccg cggcgcggac
     2341 ctgcgttacg agctggaact cacgctcgag caggccgtgt tcggcgcgaa cgtgaacatc
     2401 gacttcgcga cgctggcgga gtgcgctgaa tgcggcggca gcggctcctc gaagggatcg
     2461 aagcccgtga cctgcgacac gtgccgcggc gccggccagg tgcggatgca gcaagggttc
     2521 ttcaccgtcc agcagacctg tccgcgctgc aatggtcgag gccaggtgat cacggatccc
     2581 tgcgggtcgt gccgcggcca gggtcgcgtg cgcaagcaga agacgctgtc ggtcaaggtg
     2641 ccccagggcg tggacacggc ggaccgcatc cgcctgtccg gcgaggggga ggcgggtcgc
     2701 aatggcggtc cgccggggga cctgtacgtc gagatccgcg tcaaggacca cccgatcttc
     2761 gagcgcgacg gcagccacct gtcctgcgag gtgcccgtca gtttcgcgac agccgcgctc
     2821 ggtggcagca tcgaggtgcc gaccctcggc gggaatgcga ccatcaagat cccggcggag
     2881 acccagtcgg gccgcgtctt ccgcctgcgc gagaagggca tccgcccggt gcgcggcggg
     2941 gcggtcggcg acctgttctg ccgcatcgtg gtggagacgc ccgtgaacct gagccgcgac
     3001 cagaaggacc tgctgaggaa attcgaagat tccctgcaga aggacgcgaa gcatcaccac
     3061 ccgcgcgagg aatcgtggct cgacggggtc aagcgattct tcagcagcct tggggattga
     3121 aacgatgctg aacgtggcga tgctggggtc ctccgggcgc atgggacgca ccatcgtccc
     3181 gctggtggcc gaggccggcg acctgcagtt gagcggagcg ctgtgcgcgg ccgacgaccc
     3241 ggccgtggga cgggacgcgg gcgtgctcgc cggcgtcgct ccgctcgcgg tcgcgatctc
     3301 ggacgatccg gcgatcgcgc tggcgggcgc ccaggtcgcg atcgatttca cgctgccggc
     3361 ggtctcggtg gccaatgcgc gcctgtgccg cgagcggggg tgccccctgg tgatcggcac
     3421 cacggggcac agcgccgccc agcgcgccga gctggaagcc gtggccgaga ccctgccggt
     3481 ggtgctggcg ccgaacatga gcctcggcgt caacctgctg ttcaagctcg ccgagctggc
     3541 tgcacgcgcc ctcgacgagc agtacgacat cgagatcttc gaggcgcacc atcgcaacaa
     3601 ggtcgatgcg ccgagcggca ccgcattggg cctcggccgg gccgcggctg ccgggcgggg
     3661 cacgacgctg gagaaggcgg gcgagtacgc gcgccacggg cagaccggca tccgtgagcg
     3721 cggcaagatc ggattcagcg tcgtgcgtgg cggcgacatc gtgggcgagc accggctgat
     3781 cttcgcgggg cccggcgagc aggtcgagct ggcgcaccgc gcccaggacc ggtccggctt
     3841 cgcccgcggc gcgctggtcg cggctcgctg ggtcgtcggg cggccgcccg ggctgtactc
     3901 catgctggac gtgctcggcc tgtagnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4021 nnnnngccgc gctcgcaagt cgcgtggcgt gtcgccggtc gctcgcgtaa acttcccaac
     4081 ttagcccgtc gagccaactg gaacggggtg agcgacaagc tcatctccgg cggtcggcgc
     4141 acgcgcgaaa tgcgcctgca tgctggatgc aggcctggct aaccggaggg aatggtcgtg
     4201 ccgacacccg ctgttctggc tctcgaggac ggcaccgtgt ttcgcggcat ctccatcggc
     4261 gccgccggaa cggcgacggg cgaggtggtg ttcaacaccg cgatgacggg ttaccaggag
     4321 atcctcaccg acccgtccta tgcgcgccag atcgtcacgc tgacctaccc gcacatcggc
     4381 aacaccggga ccaatcccga cgacctcgag tcgggctcgg tgcactcggc cggtctggtc
     4441 atacgcaacc tcccgctgct gcattcgaac tggcgagcga ccgagacgct gcccgagttc
     4501 ctgacccgtg gcggcatcgt cgcgattgcc gaggtcgaca cgcgcaggct cacgcgcatc
     4561 ctgcgcgaga agggcgccca ggccggctgc atcgcgagcg gcgacgcggc gtcccgcccc
     4621 gacctcgcca tcgaggccgc gcggcgcttc cccggcctca agggcatgga cctcgccaag
     4681 gtcgtgtcga ccaagcgctc tttccagtgg aacgagggca cgaactgggc gctggagcag
     4741 ggtccgcgtc gccgcccggg acagcgcctg cacgtcgtgg cctacgattt cggcatcaag
     4801 cgcaacatcc tgcggctgct ggcagaccac gggtgccgca tgacggtggt gccagcagcc
     4861 accaccgctg ctgaagtcct ggcgctcggg cccgacggcg tgttcctgtc gaacgggccg
     4921 ggcgacccgg agccgtgcac ctacgcgatc gacgcgatca aggcgctggt cgcggcggcg
     4981 gtcccgacct tcggcatctg cctcggtcac cagctgctcg ggctggcgat gggcgcgcgc
     5041 accgtgaaga tgaagttcgg tcaccacggg gccaaccatc cggtcatcga gctcgacagc
     5101 ggccgcgtct tcatctcgag ccagaatcat ggcttcgcgg tcgacgaggc gacgctgccg
     5161 ccgaacgtcc gcgcgacgca ccgttcgctc ttcgacggct cgctgcaggg catcgaagtc
     5221 accgaccggc cggcgttcag tttccagggt caccccgagg ccagcccggg gccgcgcgac
     5281 ctcgctccgc tgttcgcgcg attcgtggaa gcgatcctgc ggcggggcat gattcccgtg
     5341 cgcaagccgg cgtgacccaa gagggcggat ccgaatgccg aagcgcaccg acatccagag
     5401 cgtactgatc atcggcgccg gcccgatcgt gatcgggcag gcctgcgagt tcgactactc
     5461 cggcgcacag gcctgcaagg cgctgcgcga ggaaggctac cgcgtcatcc tcgtcaactc
     5521 gaacccggcg acgatcatga ccgacccgga gacggccgac tcgatctaca tcgagccgat
     5581 caactggcgc acgatcgcac gcatcatcga gaaggaacgg cccgacgcac tgctgccgac
     5641 catgggcggg cagacggcgc tcaactgcgc gctggacctc gtccgcgaag gcgtgctcga
     5701 gcagttcggg gtcgagctga tcgccgcctc gcgcgaggcc atcgacatgg ccgaggaccg
     5761 cgagctgttc cgcaatgcga
//
LOCUS       DAOMFD010000124         5775 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5408_length_5775_cov_35.668531, whole genome shotgun sequence.
ACCESSION   DAOMFD010000124 DAOMFD010000000
VERSION     DAOMFD010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5775)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5775
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5408_length_5775_cov_35.668531"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..345
                     /locus_tag="PL152_07715"
     CDS             <1..345
                     /locus_tag="PL152_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR49207.1"
                     /translation="IVTHVTPDMMVAREETFGPVAPIFAFRHEKDAIRMANDTEFGLA
                     SYFYTRDLARAWRVAEGLEYGIVGLNTGLISTEVAPFGGVKESGSGREGSKYGILDYT
                     ELKYLCIGGIEG"
     gene            342..1571
                     /locus_tag="PL152_07720"
     CDS             342..1571
                     /locus_tag="PL152_07720"
                     /inference="COORDINATES: protein motif:HMM:TIGR00710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HQR49208.1"
                     /translation="MTRDISDRALLLLLAAVTALGPVATNLYLPALPAVQQYFGTSVA
                     AAQATFSVSLVTFAFGQLLWGPFSDRYGRRASILAGLAIMVCGALVSLTAQSLHWLVV
                     GRGLQAFGTATGLVTARAVVVDRHPAERMAKMLASLTMVSVLGNSLAPVAGGFLVEGL
                     GWRSVFVALVAATSSVAFFVWRRLPETRPATSRPPRSRELLATAGMLATQRMFIGCVL
                     QCVAIYATFLVFISLLPYVMVAALGRPGTEFGFYYLFIAAGYFLGNWSVGRFMLRHDL
                     HRMVVTGVWLTVAGSAAALAFAALGMTHPLWIFVPITVLAYGQGLALPNVTAAAVSLA
                     PRHAGMASSLVGFVQQLAGAACVQWMGRYPIDTALPMLTFCAAVCLAALAMLALFPRI
                     ETHRSRPVSAGSSPSAA"
     gene            1656..2753
                     /gene="hemH"
                     /locus_tag="PL152_07725"
     CDS             1656..2753
                     /gene="hemH"
                     /locus_tag="PL152_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HQR49209.1"
                     /translation="MTDYVGTGDYSHGQPSRAGILLVNLGTPDAPTPAAVRRYLAEFL
                     WDPRVIEMPRALWWLVLHGIILRIRPRRSAHAYSKVWTPEGSPLLVESRKLAAALDAR
                     LRSRHGDRVRVELAMTYGNPSIRDALERLRRGGVQRLLVLPMYPQYSSTTTASVFERV
                     TAELGRWRWLPEFRMVNQYWDDDAYVNAIAHSVEAHWQSHGRKHLLFSFHSIPKRYFT
                     AGDPYHCFCQATARRVSTRLGLADGDWSLGFQSRFGREEWLRPYLDELLHDYSHTGPR
                     RVTVVCPGFATDCLETLEEVAMQDRDLFLSHGGEAYDCVPCLNSSEAHVALYERIVAS
                     HAQGWPEFEGPADPALAEASKRRALALGAPA"
     gene            2758..3474
                     /locus_tag="PL152_07730"
     CDS             2758..3474
                     /locus_tag="PL152_07730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49210.1"
                     /translation="MLGRFLEIGVHTPDILESLAFYESLGFVQARAGDAHEHPYAVVT
                     DGRLYLGLHAAERDSPSLAWVHPGLAAHAHALQSLGIEFAYSRLGEDGLHELGFHDPS
                     GQLVVLVEARTFSPPAAGATRSSALGYFEEFGIPVSDLERAAAFWDALGLVAFDPVRT
                     PFTRLVASHRDLNIGLYGLHLPKPVLAFSDPGMAARIAALREKGLRFVERLPRGMDAG
                     ENALLEAPEGTWLLLATSLE"
     gene            complement(3523..4284)
                     /locus_tag="PL152_07735"
     CDS             complement(3523..4284)
                     /locus_tag="PL152_07735"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQR49211.1"
                     /translation="MQTKSRSWACGIALAGAVFASVLLAAGCASRRAEPVPLPPKPIG
                     EIAAQPATGTSAPPVVPAGARAYLIDGPKSVVTIRVHRAGPLAKLGHNHVVTTSQLTG
                     WAWQGRDPAGSGFEIHVPVASLVVDDPAARAAAGPGFEADVPDSARKGTYQNMLRPEV
                     LDGAAFPEVVVRAASLTGTWDDTVANADLTIRGVTRRLEVPVSVRESEGTLAASGSLR
                     ILQSDFGITPFSVAGGAIQVADELELDFAIVANAR"
     gene            4369..4929
                     /locus_tag="PL152_07740"
     CDS             4369..4929
                     /locus_tag="PL152_07740"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HQR49212.1"
                     /translation="MQWRNTASTYGVVAKFLHWTIVVLIIAQFILAEWADELPDGIDK
                     LAILSRHKSIGMLVFLLAIVRIGWRLANRGAPAPVPMPRRQQVAAAAGHGLLYLLLLA
                     QPLTGWAMSSAANYPVTLFGWVQFPPLVPASHDLHEALEELHEGTFVALATVAVLHAL
                     AALYHHFFMKDDSLRRMLPFGGRRAS"
     gene            4926..5516
                     /locus_tag="PL152_07745"
     CDS             4926..5516
                     /locus_tag="PL152_07745"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HQR49213.1"
                     /translation="MSLVGARRAGAVALVALVAALGTAAPARAATAWVMDEARSRLVF
                     TATQSGGEFDGRFQRFSADIAFDATDLAGSRFQVAIGTATADTGDPNRDSTLAGSDFF
                     ASDRWPTATYQSRQFTASAGGQFVARGTLTLRGVTRDVPLTFTFRPAADGRSAVLAGG
                     ATLRRLDFGVGQGQWEDTEWVGNDVRIRFELVLHRK"
BASE COUNT          803 a         2029 c         1960 g          983 t
ORIGIN      
        1 atcgtcacgc acgtgacgcc cgacatgatg gtcgcgcgcg aggaaacctt cggcccggtc
       61 gcgccgatct tcgcgttcag gcacgagaag gacgcgatcc ggatggcgaa cgacaccgag
      121 ttcggcctgg cgtcctactt ctacacgcgc gacctggcac gggcctggcg cgtggccgag
      181 ggactcgaat acggcatcgt gggcctgaac acggggctca tctccaccga ggtcgcgccg
      241 ttcggtggcg tcaaggagtc cggcagcggc cgcgaaggct ccaagtacgg catcctcgac
      301 tacaccgagc tcaagtacct ctgcatcggc ggcatcgagg gttgacccgc gacatttcgg
      361 atcgcgcgct gctgctgctg ctcgccgcag tgacggcgct cgggccggtc gcgaccaacc
      421 tgtacctgcc cgcgctgccg gccgtgcagc agtacttcgg cacgagcgtc gccgcggcgc
      481 aggcgacgtt ctccgtctcg ctcgtgacgt tcgcgttcgg ccagctgctg tggggcccgt
      541 tctccgaccg gtacggccgg agggcctcga tactcgcggg cctcgcgatc atggtgtgcg
      601 gggcgctcgt gagcctcacg gcgcagagcc tgcactggct cgtcgtcggg cgtggactgc
      661 aggcattcgg cacggcgacg ggcctcgtga ccgcgcgcgc ggtcgtcgtg gaccgtcacc
      721 ccgccgagcg catggcgaag atgctcgcgt cgctcacgat ggtctccgtg ctcggcaatt
      781 cactggcccc ggtcgcgggc ggcttcctgg tggaagggct cggctggcgg tcggtgttcg
      841 tcgcgctggt cgccgcgacc agcagcgtcg ccttcttcgt ctggagaagg ctgccggaaa
      901 cgcgaccggc gaccagccgt ccaccccgca gccgggagtt gctggcgacg gccggcatgc
      961 tggcgaccca gcggatgttc atcggctgcg tcctgcagtg cgtcgcgatc tacgcgacgt
     1021 tcctggtgtt catctcgctg ctcccgtacg tgatggtcgc cgcgctcggc cggcccggca
     1081 cggaattcgg cttctattac ctgttcatcg cagcggggta cttcctcggc aactggtcgg
     1141 tcgggcggtt catgctgcgc cacgacctgc accggatggt cgtcaccggc gtgtggctca
     1201 cggtcgccgg ctccgccgcc gccctcgcct tcgccgcgct gggaatgacc cacccgttgt
     1261 ggatcttcgt tccgatcacg gtgctggcct acgggcaggg gctcgcgctg ccgaacgtca
     1321 ccgcagcggc cgtcagcctc gcgccccggc acgccgggat ggcttcgagc ctggtcggtt
     1381 tcgtgcagca gctcgcgggc gccgcctgcg tgcagtggat gggccgttac ccgatcgaca
     1441 cggcgttgcc gatgctcacc ttctgcgccg cggtgtgcct cgctgcgctc gcgatgctgg
     1501 cactgttccc gcggatcgag acgcaccggt cccgtccggt gtcggcaggg tcgtcgccca
     1561 gtgccgcttg atgcctggag ccgcgccttc ctgcttttga ggccgcggca gttgcgctat
     1621 cgtgcgccgg cgtccacaag agggcacacc acgcgatgac tgactacgtc ggcaccggcg
     1681 actattctca cggccagccg tcgcgcgccg gaatcctgct cgtcaacctc ggcaccccgg
     1741 atgccccgac acccgccgcc gtcaggcgtt acctggcgga gttcctgtgg gatccccgcg
     1801 tcatcgagat gccgcgcgcg ctctggtggc tcgtgctcca cgggatcatc ctgcgcatcc
     1861 gcccgcggcg cagcgcgcac gcgtactcga aggtctggac acccgagggc tcgccgctgc
     1921 tcgtggagtc gcgcaagctc gccgcggcgc tcgatgcgcg cctgcggtcc cggcacggcg
     1981 accgcgtgcg cgtcgaactc gcgatgactt acggcaatcc gtcgatccgc gacgcgctcg
     2041 aacgcctgcg ccggggcggg gtgcagcggc tgctcgtgct gccgatgtac ccgcagtact
     2101 cctcgaccac gaccgcgtcc gttttcgagc gcgtcacggc ggagctcggc cggtggcggt
     2161 ggttgccgga gttccgcatg gtcaaccagt actgggacga cgacgcgtac gtgaacgcca
     2221 tcgcgcacag cgtcgaagcg cattggcagt cgcatggccg caagcacctg ctcttttcat
     2281 tccactcgat cccgaagcgc tacttcaccg caggcgaccc gtatcactgc ttctgccagg
     2341 cgacggcgcg ccgcgtctcg acccgactcg gcctcgcgga cggcgactgg tcgctgggct
     2401 tccagtcgcg attcgggcgt gaggaatggc tgcgtcccta cctcgacgag ctgctgcacg
     2461 actactcgca caccggcccc aggcgtgtca cggtggtgtg ccccggattc gcgaccgact
     2521 gcctcgagac gctcgaggag gtcgccatgc aggaccggga cctgttcctg tcgcacggtg
     2581 gcgaagccta cgactgcgtg ccctgcctga attcgagcga ggcgcacgtg gccctgtacg
     2641 agcgcatcgt ggcgtcgcat gcgcaaggct ggcccgagtt cgagggaccg gccgacccgg
     2701 cgctggccga ggcgtcgaag cgccgcgcgc tcgcactcgg cgcaccggcc tgagtgcatg
     2761 ctgggtcgat tcctcgagat cggcgtacat acccccgaca tcctcgagtc gctcgcgttc
     2821 tacgagtccc tcggtttcgt gcaggcccgc gccggcgacg cgcacgagca tccgtatgcg
     2881 gtggtcacgg atggacgcct gtatctcggg ctgcacgcag ccgaacgcga ctcgccgtcg
     2941 ctggcctggg tccatcccgg tctcgcggcg cacgctcacg cgctgcagtc cctgggcatc
     3001 gaattcgcct attcgcggct cggtgaggat gggctccacg agctcggatt ccacgacccg
     3061 tcgggacagc tggtcgtgct ggtcgaggcg cggacgtttt caccgcccgc cgcgggcgcg
     3121 acgcggtctt cggccctcgg ctatttcgag gagttcggca tcccggtgtc cgacctcgag
     3181 cgcgccgcag cgttctggga cgcgctggga ctggtcgcct tcgacccggt ccgcacgccg
     3241 ttcacgcggc tggtcgcgtc gcaccgtgac ctcaacatcg gcctctacgg tcttcacctg
     3301 cccaagccgg tgctcgcatt ctccgatccc ggcatggccg cccgcatcgc ggccctgcgc
     3361 gagaagggcc tgcgtttcgt cgaacgcctg ccgcggggca tggacgcggg agaaaacgcc
     3421 ctgctcgagg cgcccgaggg aacctggctg ctgctcgcga ccagcctcga gtgacgcggc
     3481 tcggcgcggc tcagaccggc gcgacgcagc gcggccctgc gttcagcgcg cgtttgccac
     3541 gatcgcgaag tccagctcca gctcgtcggc cacctggatc gcacctccgg ccacgctgaa
     3601 cggcgtgatc ccgaagtccg attgcaggat ccgtagcgat cccgacgcgg ctagcgtgcc
     3661 ctcgctctcc cgcacgctga ccggtacctc gagcctgcgg gtgaccccgc ggatcgtgag
     3721 gtcggcattc gcgaccgtgt cgtcccaggt gccggtgaga ctcgccgcgc gcacgaccac
     3781 ctccgggaac gctgcgccgt cgaggacttc gggccgcagc atgttctgat aggtgccttt
     3841 gcgcgcggag tccggcacgt ccgcttcgaa gcccggcccc gcggcggcgc gtgccgccgg
     3901 gtcgtcgacc accagcgagg ccaccggcac gtggatctcg aacccggagc cggcggggtc
     3961 acggccttgc cacgcccagc cggtgagctg gctcgtggtc acgacgtggt tgtgcccgag
     4021 cttcgcgagc ggaccggcgc gatggacccg aatggtgacg acgctcttcg gcccgtcgat
     4081 caggtaggca cgcgccccgg caggcacgac gggtggggcc gaagtgccgg tcgccggctg
     4141 tgccgcgatc tcgccgatcg gcttcggcgg cagcggcacc ggctcggcgc gacgcgaggc
     4201 gcagccagcg gcaagcagga cgctcgcgaa gaccgcgccc gccagggcga tgccgcacgc
     4261 ccacgatctg gacttggtct gcatgcgccg ccgatcgtgc acgacggacg tccgcctggc
     4321 gcgctgggtt aacatgccgc ggtcaaccag atgtcgagga tgatcaccat gcaatggcgc
     4381 aatacggcca gcacttacgg agtcgtcgcc aagttcctgc actggacgat cgtcgtcctg
     4441 atcattgcac agttcatcct tgccgagtgg gcagacgaat tgccggacgg catcgacaag
     4501 ctggccattc tctcccggca caagtccatc ggcatgctgg tgttcctgct ggcgatcgtg
     4561 cgcatcgggt ggcggctcgc gaatcgaggc gcgcccgcgc cggtgccgat gccgaggcga
     4621 cagcaggtcg cagccgcggc aggccacggg ctgctgtacc tgctcctgct ggcacaaccg
     4681 ctcaccggct gggcgatgtc gtcggctgcc aattatcccg tcaccctgtt cgggtgggtg
     4741 cagttcccgc cgctggtccc cgcaagtcac gacctgcacg aagcgctcga ggaactgcac
     4801 gaagggacct tcgtcgcgct ggccaccgtg gccgtgctgc acgcgctggc ggcgctctac
     4861 catcatttct tcatgaagga cgactcgctg cgccggatgc tgccgttcgg gggacgacgc
     4921 gcctcatgag cctcgtcggt gcccggcgtg ccggagccgt ggccctggtc gcgctcgtcg
     4981 cggcgctggg cactgcggca ccggcacggg cggccaccgc gtgggtcatg gatgaggcgc
     5041 gcagccgcct ggtgttcacg gccacccagt cggggggcga attcgacgga cggttccagc
     5101 gcttcagcgc ggacatcgcg ttcgacgcga ccgacctggc cggaagccgg ttccaggtcg
     5161 caatcgggac cgccacggcc gacaccggcg acccgaatcg cgattcgacg ctcgccggat
     5221 ccgatttctt cgcctccgac cgctggccga cggccaccta ccagtcccga cagttcacgg
     5281 cgtcggcggg tggccagttc gtcgctcgag gcacgttgac cctgcgcggc gtcacccgcg
     5341 acgtgccgct gacgttcacg ttccgacccg cagcggacgg tcgcagcgcg gtgctcgccg
     5401 ggggcgcgac tctgcgccgg ctggatttcg gcgtcggaca ggggcagtgg gaggacacgg
     5461 aatgggtggg taacgacgtc cgcatccgct tcgaactcgt tttgcaccgc aaatagaaat
     5521 caggggacat tcccctaaat tggggacatt cccctatttc gactcgaaat aggggaatgt
     5581 ccccaattta ggggaatgcg cccaattgcg cgctagaatc cgggggtcgc gagggactgc
     5641 cctcgaacca tccaggaaat aacccccggg gagggttttc accatgtcac tgcgttccac
     5701 ggcgctggat cggcgtcttg ccttgtcgtt cgcgattgct gttgcactgg ccggttgcgc
     5761 gaagaagcag gagga
//
LOCUS       DAOMFD010000125         5658 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5607_length_5658_cov_28.802427, whole genome shotgun sequence.
ACCESSION   DAOMFD010000125 DAOMFD010000000
VERSION     DAOMFD010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5607_length_5658_cov_28.802427"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..2478)
                     /locus_tag="PL152_07750"
     CDS             complement(37..2478)
                     /locus_tag="PL152_07750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49214.1"
                     /translation="MPLPSYERLGAFYLGREFDPKAGKTGDDLLYDSRDLTTHAICIG
                     MTGSGKTGLCLSLLEEAALDGVPAIAIDPKGDIANLALTFPKLEPADFQPWVDPGEAA
                     RKGKSVEEYAAATAETWRKGLADWNEDGARIQRLRDAAEVTIYTPGSESGRPLSILRS
                     FSAPDATTLGDATALKERIGSAVAGLLGLLGIDADPIKSREHILLSAILEASWRKGQS
                     PDLAAVIQAVQKPPFDKIGVFDLESFYPAKERTELALRINGLLASPGFSAWLAGDPLD
                     VQKLLYTESGKPRIAVISIAHLSDAERMFVVTLVANELVAWMRRQSGTTSLRALFYMD
                     EVFGYFPPSAMPPSKLPLLTLMKQARAFGLGVVLATQNPVDLDYKGLGNAGTWFIGRL
                     QTERDKNRVIEGLLGTDAAGGMDRESLAGLMSSLAQRTFLMRNVHDDAPVLMQTRWAL
                     CYLRGPLTLAEITKLQGSTSGGRSAADANANAGPVADAAASQPSAAATASGSSAAARG
                     AQAQDATAGAGRPVVQAGVSEKFLRAPPGKGAVTYQPRLGARARAHFVDAKSGMNAWE
                     SWYYVATVGKDGPDWDTAEVIPEPGPDFDDEPRADASFGEAPAALLSNRGYKAWTDAL
                     EDHVYRNASLNVMSCPALKMSAAPGGTEGEFRAHIAQALREKRDAAVEALRAKYASKL
                     AGLQAREERAEQRVEREKSQATSATLATAVSVGGSLLGALFGGRRGSTFSKVSTAARG
                     IGRATQQRADVTYAESDARAVQEQIAAVNAELEAEIERLGGEFDPNSVRIETVAIRPK
                     KSDVTVEDMALVWRP"
     gene            2641..4380
                     /locus_tag="PL152_07755"
     CDS             2641..4380
                     /locus_tag="PL152_07755"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR49215.1"
                     /translation="MSIALDQVTKRYRSVPVVNDVSLKVDQGEFLVLLGPSGSGKSTL
                     LRAIAGLTDIDHGRVSLHGRDVTHVSARHREVGFVFQHYALFGHMTVGENIEFALRVR
                     RVKAAERRARRKELLRLVALEGMDDRLPTQLSGGQQQRIAVARALAHRPQVLLMDEPF
                     GALDAKIREELRTTIRQIQRDLGMTTILVTHDQEEAFALADRIGVMNQGRLLECGRPD
                     DLYTRPQTRFVSTFLGAANLVLGYRTAAGLRFSAEVAAGEAVPREVVAVLRPEEVELG
                     ASQDVVRSNFVGYGAVEEVLFGGAVERLRVRMSDDGPVAVAPGREEHSDSGSLLHVSR
                     TLPEQRSFPVVPGQRVAVGARRVHVLPTPISSFTVVADTPEAARALHDSPLLSTLRVR
                     MNARIATRIGEVARAIPGMPTLATGPGSCDAAEWQVRNGARQLLCVPPSAPLPQHVVI
                     HTLDPEARRATVPVAASLLRHVAAEAVYLAIHAEPSSERERAEYTRDLLDARSAALAG
                     HGLDMRTELRIGREAEELQKELDAHPNSMLVLGTSDPERIDWTWMRGMLEGVPGRPVL
                     IVCAPPADGTREH"
     gene            4384..5223
                     /gene="cysT"
                     /locus_tag="PL152_07760"
     CDS             4384..5223
                     /gene="cysT"
                     /locus_tag="PL152_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter permease subunit CysT"
                     /protein_id="HQR49216.1"
                     /translation="MSWFRKPSILPGFGLTLGFTTFFLSGVVLLPLAALTFKAASLTW
                     PEFAGILLDPRSLASYRLSFGAALLAAAVNSVFGFIVAWSLVRYDFPGRRLVDALIDL
                     PFALPTAVSGIALATVFAPTGWLGQKLATFGVQIAYTWIGVVVALTLIGLPFVVRSVQ
                     PALIEVQRDLEEAAETLGASPITIFRRIILPAVLPALLTGFTLALARGIGEYGSVVFI
                     SGNLPFKTEITPLLIVIKLEQFDYGGAAALGFIMLVMSFVMLFAINLVQAWNRRAHAG
                     RER"
     gene            5225..>5658
                     /gene="cysW"
                     /locus_tag="PL152_07765"
     CDS             5225..>5658
                     /gene="cysW"
                     /locus_tag="PL152_07765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Part of the ABC transporter complex cysAWTP
                     involved in sulfate/thiosulfate import; Derived by
                     automated computational analysis using gene prediction
                     method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HQR49217.1"
                     /translation="MAGLAPRVERRRHEPMSARVARVVLIGVSIAFLSLLLLAPLGAV
                     LAQALAKGLAAYAESFTDPDTLASIKLTLFTAAIVVPANTVFGICAAWAIAKFEFRGK
                     SLLITLIDLPFAVSPVVAGLVLVSLFGVHGWFGEWLQDRDIA"
BASE COUNT          774 a         1954 c         1978 g          952 t
ORIGIN      
        1 tcgttcatcg ccggtcctcc ccgcgcggat tctacgtcaa ggccgccaga ccagcgccat
       61 gtcctcgacc gtaacgtccg acttcttcgg tcgtatggcg accgtttcga tgcgcacgga
      121 attcggatcg aactcgcccc ccagccgctc gatctcggcc tcgagctcgg cattcaccgc
      181 cgcgatctgt tcctgcaccg cgcgggcatc ggattccgca taggtcacgt cggcccgctg
      241 ctgggtcgcg cgcccgatgc cgcgagcagc ggtcgacacc ttgctgaacg tgctgccgcg
      301 ccgcccgccg aacagggccc cgagcaggct gccgccgacc gacaccgcgg tggcgagggt
      361 cgcgctcgtg gcctgtgact tctcacgctc gacgcgctgc tcggcccgct cttcacgtgc
      421 ctgcagcccg gcgagtttcg acgcgtactt ggcccgcagc gcctcgacgg cggcgtcccg
      481 tttctcgcgc agcgcctgtg cgatgtgagc gcggaattcg ccctccgtgc cgcccggcgc
      541 agcgctcatc ttgagcgcag ggcacgacat cacgttgagc gacgcgttgc ggtagacgtg
      601 gtcttcgagc gcgtcggtcc aggccttgta gccgcggttg gagagcaggg ctgcgggcgc
      661 ctcgccgaac gaagcatcgg cgcgcggctc gtcgtcgaaa tcgggtcccg gttccgggat
      721 cacctcggcc gtgtcccagt cgggcccgtc cttgccgacg gtggcgacgt agtaccagga
      781 ttcccacgca ttcatgccgg acttcgcgtc cacgaagtgc gcgcgagccc gcgcgcccag
      841 ccgtggctgg taggtcaccg cccccttgcc cggcggcgcc cgcaggaact tctcgctgac
      901 gccggcctgc acgacgggcc ggccggcccc ggcggtcgcg tcttgcgcct gcgctccacg
      961 cgcagctgcg gacgagccag acgccgtggc ggcggccgac ggttgactcg cagccgcatc
     1021 ggcgacaggg ccggcatttg cgttcgcgtc ggcagcgctg cgtccgccac tggtcgagcc
     1081 ttgaagtttc gtgatctccg ccagcgtcag cgggccccgc aggtagcaaa gcgcccagcg
     1141 cgtctgcatc agcaccggcg cgtcgtcgtg cacgttgcgc atcaggaacg tgcgctgcgc
     1201 gaggctcgac atcagtcccg cgagcgattc gcggtccatg ccaccggccg catccgtgcc
     1261 tagcaggccc tcgatcacgc ggttcttgtc gcgctcggtc tgcaggcggc cgatgaacca
     1321 cgtgccggca ttgccgaggc ccttgtagtc gaggtccacc gggttctgcg tggcgagcac
     1381 cacgccgagg ccgaaggcgc gtgcctgctt catgagcgtg agcagcggca gcttggacgg
     1441 gggcatcgcg gacggcggga agtagccgaa cacctcgtcc atgtagaaga gcgcacgcag
     1501 actggtcgtg cccgactgcc ggcgcatcca cgcgacgagt tcgttggcga ccagcgtgac
     1561 gacgaacatg cgctcggcgt cgctcaggtg cgcgatcgag atcacggcga tgcgcggctt
     1621 gcccgactcg gtgtagagga gcttctggac gtcgagcggg tcgccggcga gccacgcgct
     1681 gaatccaggc gacgcgagca ggccgttgat ccggagcgcc agctccgtgc gttccttcgc
     1741 cgggtagaag ctctcgaggt cgaagacgcc gatcttgtcg aacggcggct tctgcacggc
     1801 ctgaatcacg gcggcaaggt ccgggctctg cccctttcgc cagcttgcct cgagaatggc
     1861 cgacagcagg atgtgctcgc gactcttgat cgggtcggca tcgatgccga gcagtccgag
     1921 cagtcccgcc accgcggagc caatgcgctc cttgagcgcc gtggcgtctc ccagggtcgt
     1981 ggcgtcgggc gccgagaacg agcgcaggat ggacagcggc cgcccggact ccgatcccgg
     2041 cgtgtagatg gtcacttcgg cggcgtcgcg cagtcgctgg atacgcgcgc cgtcctcgtt
     2101 ccagtcggcc aggcccttgc gccaggtctc ggcggtcgcc gcggcgtact cctcgaccga
     2161 cttgcccttg cgcgcggctt cgcccgggtc cacccagggc tggaaatccg cgggctcgag
     2221 tttcggaaag gtgagcgcga gattggcgat gtcgcccttg gggtcgatgg cgatcgccgg
     2281 cacgccgtcg agcgcggcct cctcgagcag cgacaggcac aggcccgtct tgccgctgcc
     2341 ggtcatgccg atgcagatcg cgtgggtggt gaggtcgcgc gagtcgtaga gcaggtcgtc
     2401 gccggtcttg cccgccttgg ggtcgaattc ccggccgagg tagaaggcgc cgaggcgctc
     2461 gtaggagggg agtggcatgg aaatcggggt gtgcgcgata gtgatggggg gtagtgtggg
     2521 gcaggaaagt gaacggtggc tagatagccc ctgtcaccgc tttgttacct gccggcgact
     2581 gacggcgtaa cctcgggccc ctaggatgac cggtccgggg ggcctgcaag cggattcacc
     2641 atgtccatcg cgctcgacca ggtgacgaaa cggtaccggt ccgtcccggt cgtcaacgac
     2701 gtttccctca aagtcgacca gggcgagttc ctggtgctgc tgggacccag cggcagcggc
     2761 aagagtacgc tgctgcgcgc gatcgccggg ctcacggaca tcgaccatgg ccgcgtctcg
     2821 ctgcacgggc gggacgtgac gcacgtgtcc gcgcgtcacc gcgaggtcgg gttcgtgttc
     2881 cagcactatg cgctgttcgg ccacatgacg gtcggcgaga acatcgagtt cgcactgcgg
     2941 gtccgcaggg tcaaggccgc cgagcgccgc gcccgccgca aggaactgct gcgactcgtc
     3001 gcactggaag gcatggacga ccgcctgccg acccagctgt cgggcgggca gcagcagcgc
     3061 atcgcggtgg ccagggcgct cgcgcaccgg ccccaggtgc tgctgatgga cgaaccgttc
     3121 ggcgcgctcg atgccaagat ccgcgaggag ctgcgcacga ccatccggca gatccagcgc
     3181 gacctgggca tgacgacgat cctggtgacg cacgaccagg aggaagcctt tgcgctcgcc
     3241 gaccgcatcg gcgtgatgaa ccagggccgc ctgctcgaat gcggccgccc cgacgatctc
     3301 tacacccggc cgcagacacg tttcgtctcg acgttcctcg gcgccgcgaa cctggttctc
     3361 gggtaccgga ccgcggccgg tctgcggttc agcgccgagg tggctgcggg tgaggcggtg
     3421 ccccgggagg tcgtcgccgt cctgcggccc gaggaagtcg agctcggtgc gtcccaggac
     3481 gtcgtgcgct ccaatttcgt cggctacggt gccgtcgagg aagtgctgtt cgggggcgcg
     3541 gtcgagcgac tccgggtgcg gatgtcggac gatggaccgg tcgccgtggc accaggtcgc
     3601 gaggagcact cggatagcgg atcgctgctg cacgtgtcgc gcacgctgcc cgagcagcga
     3661 tccttcccgg tcgtcccggg acagcgcgtc gcggtgggcg cgcggcgcgt gcacgtgctg
     3721 ccgaccccga tctcgagctt caccgtcgtg gcggacacgc cggaggccgc gcgggcgctc
     3781 cacgactcgc cactgctgtc gacgttgcgc gtccgcatga acgcgcgcat cgccacgcgc
     3841 atcggcgagg tcgcgcgtgc gatccccggc atgccgacgc tcgcgaccgg accgggcagt
     3901 tgcgatgctg cggagtggca ggtccgcaac ggggcgaggc aactgctgtg cgtgccgccc
     3961 tcggcgcccc tgccgcagca cgtcgtgatc cacacgctcg atccagaggc gcgacgtgcg
     4021 accgtcccgg tcgccgcgag cctgctgcgc cacgtcgcgg ccgaggccgt ctacctcgcc
     4081 attcatgcgg agccgtcgtc ggagcgggag cgtgccgaat acacgcgcga cctgctcgac
     4141 gcgcgctcgg ccgcgctcgc cggccacggc ctcgacatgc gcactgagct gcgcatcggc
     4201 cgcgaggccg aggaactgca gaaggaactc gacgcgcacc cgaactcgat gctcgtgctc
     4261 ggcacgtcgg atcccgagcg tatcgactgg acctggatgc gcgggatgct ggaaggcgtg
     4321 cccggccggc cggtgctgat cgtctgcgcg ccgcccgcgg acggcacccg ggagcattga
     4381 ccggtgtcgt ggtttcgcaa gccgagcatc ctgccgggct tcggcctgac cctgggcttc
     4441 accaccttct tcctgagcgg cgtggtgctg ctgccgctgg ccgccctcac gttcaaggcc
     4501 gcgtcgctca cgtggcccga gttcgccggc atcctgctcg acccgcgctc gctggcgtcg
     4561 taccggctga gcttcggcgc cgcgctgctc gccgcggccg tgaattcagt gtttgggttc
     4621 atcgtcgcgt ggtcgctggt gcgctacgac ttccccggac gccgtctcgt cgatgccctg
     4681 atcgacctgc cgttcgcact gccaaccgcg gtgtccggca tcgcgctcgc aacggtgttc
     4741 gcgccgacag gctggctcgg acagaagctt gcgacgttcg gcgtgcagat cgcctacacc
     4801 tggatcggcg tggtcgtcgc gctaacgctg atcggcctgc cgttcgtcgt gcgctcggtg
     4861 cagccggcgc tgatcgaggt ccagcgcgac ctcgaggagg cggcggaaac gctcggtgca
     4921 agccctatta ccatcttccg ccgcatcatc ctgcctgccg tgctgcccgc gctgctcacg
     4981 ggcttcacgc tggcgctggc acgcggaatc ggcgagtacg ggtcggtggt gttcatctcc
     5041 ggcaacctgc cgttcaagac cgagatcacg ccgctgctga tcgtgatcaa gctcgagcag
     5101 ttcgactacg gcggggcggc cgcgctcggc ttcatcatgc tggtcatgtc gttcgtgatg
     5161 ctgttcgcga tcaacctcgt gcaggcctgg aaccggcgcg cgcacgcggg gcgggagcgc
     5221 tgacgtggcg ggcctcgcac cccgggtcga gcgacggcgc cacgagccga tgtccgcgcg
     5281 cgtcgccagg gtcgtgctca ttggcgtgtc gatcgcattc ctgtcgctgc tgctgctcgc
     5341 gccgctcggt gccgtgctcg cgcaggcgct ggccaagggg ctcgccgcct acgcggagag
     5401 cttcacggat cccgacacgc tcgcgtcgat caagttgacg ctgttcacgg cagccatcgt
     5461 ggtgccggcc aacaccgtct tcggcatctg cgcggcgtgg gcgattgcca agttcgagtt
     5521 ccgcggcaag agcctgctca ttacgctcat cgacctgccg ttcgccgtgt cgccggtggt
     5581 ggcgggcctg gtgctcgtca gtttgttcgg cgtgcacggc tggttcggcg agtggctgca
     5641 ggaccgcgac atcgcgat
//
LOCUS       DAOMFD010000126         5585 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5719_length_5585_cov_30.868535, whole genome shotgun sequence.
ACCESSION   DAOMFD010000126 DAOMFD010000000
VERSION     DAOMFD010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5719_length_5585_cov_30.868535"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..343)
                     /gene="ccsA"
                     /locus_tag="PL152_07770"
     CDS             complement(<1..343)
                     /gene="ccsA"
                     /locus_tag="PL152_07770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQR49218.1"
                     /translation="MWTWLHKFASPPHAYRLLGQLAPWFGWPALGLILLAAWWGLVKA
                     PADYQQGDAFRILYVHAPSAWMSMFTYVVMAVAAAIGLVWRIKLAHAAAAAAAPIGAS
                     FTFLALATGSIW"
     gene            complement(377..1063)
                     /gene="ccmB"
                     /locus_tag="PL152_07775"
     CDS             complement(377..1063)
                     /gene="ccmB"
                     /locus_tag="PL152_07775"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HQR49219.1"
                     /translation="MRRASVSAAARLVFRRDLTLAWRRWDEVAQPLIFYVVVTTLFPL
                     ATTPDLQQLRQIAGGVVWVAALLASLLALESLFRADVEDGTMEQWVLTGQPLGWLLLA
                     KVACHWVLTGLPLVVISPIVGTALGLPFEAWGTLVVSLLLGTATLSVLGGIGAALTVG
                     VRRGSVLLSLLVLPLAMPLLIFGSRAVTMAMDGDSPAGPLQLLAAIFFLVLSLGPLGM
                     SAAIRISVET"
     gene            complement(1063..1692)
                     /gene="ccmA"
                     /locus_tag="PL152_07780"
     CDS             complement(1063..1692)
                     /gene="ccmA"
                     /locus_tag="PL152_07780"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF010061.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HQR49220.1"
                     /translation="MVPDGAVLEARAINAWRGDRHILKGVSFVLRAGEFLKVTGPNGV
                     GKTTLLRVVCGLLPAETGEIDWCGKPVRGASDEFHAQLGYLGHPNSLKADLTARENLK
                     YLAGLRQTLTPKEVDAALDRCGILSRGDLPARSLSAGQKRRLALARLLLAEASLWVLD
                     EPVTNLDTAGITLVEELIQEHVQRGGLAFAAAHQRLLDHSPNLRRLELG"
     gene            complement(1783..>2178)
                     /locus_tag="PL152_07785"
     CDS             complement(1783..>2178)
                     /locus_tag="PL152_07785"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49221.1"
                     /translation="RLVGSLDGQPFAAGIEERPDAFVVRRQCLRFEFHEDTGALHHAS
                     AEHEGHLRAPMPGHVLDVRAVAGRRVAAGDVLVVLEAMKMEHALAAPWDGTVISVPVK
                     SGDRVEEGVELVILEPLEEAPLRHTAAQE"
     assembly_gap    2179..2278
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2279..3528)
                     /locus_tag="PL152_07790"
     CDS             complement(<2279..3528)
                     /locus_tag="PL152_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HQR49222.1"
                     /translation="MFQSLLIANRGEIACRVIRTARHLGVRTIAVHSEADRGALHTRL
                     ADIAVEIGPAAARDSYLNVERVLDAARQTGAQAIHPGYGFLSENAAFARACDAAGVVF
                     VGPPADAILRMGSKSEARRLMAAAGVPVLPGYDGDDQTLERLRDESNRLGFPLLIKPT
                     AGGGGKGMRIVRSAPELPDALAGARREASRSFGDERVLLERFVEKGRHVEIQVFADAH
                     GGAVHLFERDCSLQRRHQKVIEEAPAPGLDEATRAAMGAAAVAAARAVDYRGAGTVEF
                     LFDGREFYFLEMNTRLQVEHPVTEMITGLDLVEWQLRVAGGDRLPLEQSAIRRHGHAM
                     EARLYAEDPERGFLPSTGRLRRLRLPDGLPDVRVDSGVEEGDEVTVHYDPMIAKVVAW
                     GADRGAAVALLRKALERTDVEGPHT"
     gene            complement(3535..5142)
                     /locus_tag="PL152_07795"
     CDS             complement(3535..5142)
                     /locus_tag="PL152_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HQR49223.1"
                     /translation="MSVIDSRVDPGSAEFAGNDAVNRSLARELRELVRRVKAGGSAAA
                     RKQHEERGKMFVRDRVDRLLDPGSPFLEIGQLAGHELYGDWIPSGGVVAGIGRVSGLE
                     CVIVANDATVKGGTYYPITVKKHLRAQDVALENRLPCIYLVDSGGAFLPRQDEVFPDR
                     EHFGRIFYNQAQMSARGIPQLAVVMGSCTAGGAYVPAMCDESIIVREQGTIFLGGPPL
                     VQAATGERVDAETLGGGDVHARQSGVVDHLADNDAHALWLARRLVARLNRQGSRPPPV
                     AERLPRHAADTLYGVVPRDTRHPYDVREVIARLVDASELDEFKALYGTTLVCGFASIG
                     GYPVGIVANNGVLFSESALKGAHFVELCNRRRVPLVFLQNITGFMVGRKYEAGGIARD
                     GAKMVTAVSCASVPKFTVVIGGSFGAGNYAMCGRAYGGRFLWSWPNARISVMGGEQAA
                     TVLAIVRREGMQRRAEEWPAADEQAFRDQIRGQYETQGHPYYASARLWDDGVIDPADT
                     RDVLKLALAAATCRPDEGETPYGIFRM"
     gene            complement(5181..>5585)
                     /locus_tag="PL152_07800"
     CDS             complement(5181..>5585)
                     /locus_tag="PL152_07800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005404324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR49224.1"
                     /translation="GPLGLMRASLDLVLPYVHERKQFGQPIGSFQLMQGKLADMYAAT
                     NSSRAYVYSVASACDRGRTTRMDAAACILLASEQATAVALHAIQALGGNGYINDFPAG
                     RILRDAKLYEIGAGTSEIRRMLIGRELFAATT"
BASE COUNT          868 a         1967 c         1863 g          787 t
ORIGIN      
        1 cccagatgga gccggtggcg agcgcgagga acgtgaacga ggcgccgatc ggcgccgcgg
       61 cggccgcggc ggcgtgcgcc agcttgatgc gccagacgag cccgatggcc gccgcgacgg
      121 ccatcacgac gtaggtgaac atcgacatcc acgcgctcgg ggcgtgcacg tacaggatgc
      181 ggaatgcgtc gccctgctgg tagtcggcgg gcgccttcac gagcccccac cacgcggcga
      241 gcaggatcag acccagggcc ggccagccga accagggcgc cagctggccg agcaggcggt
      301 acgcgtgcgg aggcgaagcg aacttatgca accaggtcca catggaggtc cttgtcgatg
      361 actcttgtac gtgccgtcag gtctctacgc tgatccggat cgcggccgac atacccaggg
      421 ggccgaggct cagcaccagg aagaagatcg cggcgagcaa ctgcagcggc ccggccggcg
      481 aatcgccgtc catcgccatg gtcacggccc gcgacccgaa gatgagcagc ggcatcgcga
      541 gcggcagcac cagcagcgac agcagcacgc tgccgcgtcg cacgccgacg gtgagcgcag
      601 cgccgatccc gcccaggacg ctgagcgtgg ccgtgccgag cagcagcgat acgacgagcg
      661 tgccccaggc ttcgaacggc aggcccaggg ccgtgccgac gatcggggaa atcacgacga
      721 gcggcagccc ggtcagcacc cagtgacagg ccaccttggc cagcagcagc catcccagtg
      781 gttgtccggt cagcacccat tgctccatcg tgccgtcttc gacatccgcg cggaacaaag
      841 attccagcgc gagcaacgaa gcgagcaatg cggcaaccca cacgacaccg cccgcgatct
      901 ggcgcagctg ctgcaggtcc ggtgtcgtgg ccagggggaa cagcgtagtg acaactacgt
      961 agaagatcag tggctgcgcg acctcgtccc agcggcgcca ggcgagcgtg aggtcgcgcc
     1021 ggaacacgag ccgggcggcg gcggaaaccg aggcgcgacg catcagccga gctccagccg
     1081 acgcaggttc ggcgaatggt ccagcaggcg ctggtgtgcc gccgcgaacg cgagcccgcc
     1141 gcgctgcacg tgttcctgta tgagttcctc gaccagcgtg atgccggcgg tgtcgagatt
     1201 cgtgacgggt tcgtcgagca cccagaggga ggcctcggcg agcagcaggc gagcgagcgc
     1261 gagacgccgc ttctggccgg ccgacagcga gcgggccggc aggtcgcccc gggaaaggat
     1321 gccgcagcga tcgagcgccg catccacctc cttgggcgta agggtctggc gcaggccggc
     1381 gaggtatttc aggttctccc gggcggtcag gtccgccttg agcgagttag ggtgacccag
     1441 gtagccaagc tgggcgtgaa attcgtcgct cgcgccccgc acgggcttgc cacaccagtc
     1501 gatctccccg gtctcggcgg gcagcaggcc gcagaccacg cgcagcagtg tcgtcttgcc
     1561 gaccccgttg gggccggtga ccttgaggaa ctcgcccgca cgcaagacga acgacacacc
     1621 cttcaggatg tgcctgtcac ctcgccacgc atttatcgct cgagcctcga gcaccgcccc
     1681 atcgggcacc atggatcctc cgccgactct cgcacggtcc ggtcgcccgg tacccgagcg
     1741 gcggggcgac ttaagtcggc gcgcagtcta gcaaatcgag aatcattcct gcgctgcggt
     1801 atgccgcaag ggggcctctt ccagcggctc gaggatgacc agttcgaccc cctcctctac
     1861 gcggtctccc gacttgaccg gcaccgaaat cacggttccg tcccagggcg ccgccagggc
     1921 gtgctccatc ttcatcgctt cgaggacgac cagtacgtcg ccagccgcga cccgccgccc
     1981 cgcgactgcc ctgacgtcca ggacgtgccc cggcatcggc gcccgcaggt gcccttcgtg
     2041 ctcggccgac gcatggtgca gcgcgccggt gtcctcgtgg aattcgaagc gcaggcactg
     2101 gcgccgcacc acgaaggcat ccggtcgctc ctcgatgccc gccgcgaacg gctggccgtc
     2161 cagcgatccc accagccgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngt
     2281 gtgcgggcct tcgacgtccg tccgctccag agccttgcgc aacagggcga cggccgcacc
     2341 acggtcggcg ccccaggcga cgactttggc gatcatcggg tcgtagtgca ccgtcacctc
     2401 gtcgccctct tcgacgccgc tgtccacgcg gacgtccggc agcccgtccg gcagccgcag
     2461 gcgacgcagg cggccggtgg acggcaggaa cccgcgttcc gggtcctccg catacagcct
     2521 cgcttccatc gcgtgtccgt ggcggcggat ggccgattgc tcgagcggca accgatcgcc
     2581 gccggccacg cgcaactgcc attcgacgag gtcgaggccc gtgatcatct cggtcaccgg
     2641 gtgctcgacc tgcagccgcg tgttcatctc caggaaatag aactcgcggc cgtcgaacag
     2701 gaactcgacg gtgcccgcgc cgcggtaatc gacggcgcgc gccgccgcga cggccgctgc
     2761 gcccatcgcg gcgcgcgtcg cctcgtcgag gcccggggcc ggcgcctcct cgatcacctt
     2821 ctggtgacgg cgctgcagcg aacagtcgcg ctcgaacagg tgcaccgccc cgccatgggc
     2881 gtcagcgaac acctggatct cgacgtgcct gcccttctcg acgaagcgct cgagcagcac
     2941 gcgctcgtcc ccgaacgacc tggacgcctc gcgtcgcgcg ccggcgagcg cgtcaggcag
     3001 ttccggcgcg ctccgcacga tgcgcatgcc cttgcctccg ccccccgccg tcggcttgat
     3061 cagcagcggg aagccgagcc tgttcgattc atcgcgcagt cgctccagag tctggtcgtc
     3121 gccgtcgtac cctggcagca ccgggacgcc ggccgcggcc atcagtcgcc gcgcctcgct
     3181 cttggaaccc atgcgcagga tggcgtccgc aggcggaccg acgaagacga ccccagccgc
     3241 gtcacaggcg cgcgcgaacg cggcgttctc cgacaggaaa ccgtatccgg gatggatggc
     3301 ctgtgcgcca gtctgccgcg cggcgtcgag gacgcgctcg acgttcaggt agctgtcgcg
     3361 ggccgcggcg ggcccgatct cgacggcgat gtccgcgagc cgtgtgtgca gggcaccccg
     3421 gtccgcttcc gaatgcaccg caatcgtgcg cacgccgaga tggcgcgcgg tgcggatgac
     3481 gcggcaggcg atttcgccgc gattggcgat gagcaggctc tggaacatgc gccgctacat
     3541 ccggaagatc ccgtacgggg tctcgccctc gtccgggcgg caggtcgccg cggcgagcgc
     3601 gagcttcagc acgtcgcgcg tgtccgccgg gtcgatgaca ccgtcgtccc acaggcgcgc
     3661 gctcgcgtaa tacgggtgac cctgggtctc gtactggccg cggatctggt cgcgaaacgc
     3721 ctgttcatct gccgccggcc actcctccgc tcgtcgctgc atgccttccc gcctgacgat
     3781 cgcaagcacg gtcgcggcct gctcgccgcc catcacggag atccgcgcgt tgggccagct
     3841 ccacaggaag cggcccccgt aggcgcggcc gcacatcgcg tagttgccgg cgccgaacga
     3901 accgccgatg acgacggtga acttcggcac cgacgcgcac gagaccgcgg tcaccatctt
     3961 cgccccgtcg cgggcgatgc cgccggcctc gtacttgcga ccgaccatga agcccgtgat
     4021 gttctgcagg aacacgagcg ggacgcggcg tcgattgcac agttcgacga agtgcgcgcc
     4081 cttgagcgcc gactcggaga acagcacgcc gttgttcgcc acgatcccca ccggataccc
     4141 gccgatgctc gcgaacccgc acaccagcgt ggttccgtac aacgccttga actcgtcgag
     4201 ttcggaggcg tccacgagac gcgcgatcac ttcgcgtacg tcgtacgggt gtcgggtatc
     4261 gcgcggcacg acaccgtaga gcgtgtcggc cgcgtggcgt ggaagtcgtt cggccaccgg
     4321 cggcggccgc gaaccctgcc ggttcaggcg tgccacgagc cggcgcgcca gccacagcgc
     4381 atgcgcgtca ttgtcggcga ggtggtcgac cacgccggac tgcctggcgt gcacgtcacc
     4441 gccgccaagc gtctccgcgt ccacgcgctc gccggtggcc gcctgcacga gcgggggccc
     4501 gccgaggaag atcgtgccct gctcccgcac gatgatggac tcgtcgcaca tcgccggcac
     4561 gtacgcaccg ccggcggtgc acgaccccat taccacagcg agttgcggaa tcccacgggc
     4621 cgacatctgc gcctggttgt agaagatgcg gccgaagtgc tcgcggtccg ggaacacttc
     4681 gtcctggcgc ggcaggaacg ccccgccgga atccacgagg tagatgcacg gcaggcggtt
     4741 ctcgagcgcg acgtcctgcg cccgcaggtg cttcttcacc gtgatcgggt agtaggtccc
     4801 gcccttgacg gtcgcgtcgt tcgcgacgat gacgcattcg aggccgctca cgcgcccgat
     4861 gccggccacg accccgccgg aggggatcca gtcgccgtac aactcgtggc ccgccagctg
     4921 gccgatctcg aggaagggcg accccggatc gagcaggcgg tcgacgcggt cgcggacgaa
     4981 catcttgcct cgctcttcgt gctgcttgcg cgccgcggcg gagccgccgg ccttcacccg
     5041 ccgcacgagt tcgcgcagct cgcgggcaag gctgcgattg acggcgtcgt tgccggcaaa
     5101 ctccgcagag cccgggtcga cgcggctgtc gatcacgctc acgtgcccac ctccaccgcg
     5161 atgacggctg cgccggacat tcaggtggtc gccgcgaaca gttcgcgccc gatcagcatg
     5221 cgccggatct cgctggtgcc ggcgccgatc tcgtagagct tggcatcgcg caggatccgg
     5281 cccgccggga agtcgttgat gtagccgttg ccgccgagcg cctggatggc gtgcagcgcg
     5341 acggcggtcg cctgctcgga ggcgagcagg atgcaggccg cggcgtccat gcgcgtcgtg
     5401 cgaccgcggt cgcacgcact cgcgaccgag tagacgtagg cgcgggacga gttcgtcgcc
     5461 gcgtacatgt cggcgagctt cccctgcatg agctggaagc tgccgatcgg ctggccgaac
     5521 tgcttgcgtt cgtggacgta gggcaggacg aggtcgaggc tggcgcgcat cagtcccagc
     5581 ggtcc
//
LOCUS       DAOMFD010000127         5580 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5732_length_5580_cov_31.766516, whole genome shotgun sequence.
ACCESSION   DAOMFD010000127 DAOMFD010000000
VERSION     DAOMFD010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5580)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5580
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5732_length_5580_cov_31.766516"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            55..744
                     /gene="rnc"
                     /locus_tag="PL152_07805"
     CDS             55..744
                     /gene="rnc"
                     /locus_tag="PL152_07805"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HQR49225.1"
                     /translation="MLTSGAWSEARLGYRFGDPALLDAALTHRSAGRANYERLEFLGD
                     AVLNFAVAMLVYRTYPDADEGDLSRYRASLVSGESLADIAGGLGLGEQLRLGSGELKT
                     GGFRRRSILADALEALFGAIYLDGGHTAVATVIESLFAGRLAVLPEARDLKDPKTRLQ
                     ELLQGKAEPLPSYALEDVSGEPHEHWFVASCEVASLGLQERGEGTSRRRAEQAAAQRV
                     LDLLAERGLGQ"
     gene            741..1661
                     /gene="era"
                     /locus_tag="PL152_07810"
     CDS             741..1661
                     /gene="era"
                     /locus_tag="PL152_07810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HQR49226.1"
                     /translation="MTGQAQAGHTARCGFAALVGRPNVGKSTLLNALLGRKVSIVSPR
                     PQTTRTRIHGVLTRPGMQIVFVDLPGIHAKQPRAINRYMNRTALASLADSDVNLFVIE
                     ALRWTEEDERALAELRAAGRPILLLINKSDRAQPRSRLLPFIAEVSAKADFAQVVPLS
                     ALKRKNLDRLPELIAGYLPESPLLFPPEQVTDVSDRFMAAEIVREKLTRRLQEEVPYG
                     LVVEVEGMGPAEDEPGKLVIQAVIWVERAGQKAIVIGKGGELLKEVGRAARLDLKHYF
                     GKPVHLELWVKVREGWSDDESALRRFGYEA"
     gene            1658..2401
                     /gene="recO"
                     /locus_tag="PL152_07815"
     CDS             1658..2401
                     /gene="recO"
                     /locus_tag="PL152_07815"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HQR49227.1"
                     /translation="MSGAARRVQLQPAYLLHHYAWRDSSRILELLTRDHGRVSVFARA
                     ARRGGSSLPAALQPFSALLVSWTARGEAGQLTGAEAAAPPARIDGDRLMSAFYANELL
                     VKLLPRHDPHPALYDSYATLLERLADATRRPSRALRLFEKRLLDELGWGLSLESDASG
                     RPIDVGGSYRYRVDGGAERLDGVAEGSLVFSGASLVSLAREELGDDRSLADARRLLKA
                     ALDQCLDGRPLRTREVMNAMRARGGAQGG"
     gene            2405..3157
                     /gene="pdxJ"
                     /locus_tag="PL152_07820"
     CDS             2405..3157
                     /gene="pdxJ"
                     /locus_tag="PL152_07820"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HQR49228.1"
                     /translation="MLTHPLKLGVNIDHVATLRQVRRAIYPDPLYAALIAEQSGADSI
                     TLHLREDRRHIQDHDVTRMRESLQTRMNLEMAATEAMVRFAAGLRPQDCCLVPERREE
                     LTTEGGLDVAGQVDRLKDCCAALDDAGIRVSLFIDPEPEQLEAALDAGAPVVELHTGA
                     YADATGERQAMELARLVEAAQFGARIGLTVHAGHGLNYHNVQPVAAIPEIVELNIGHA
                     IVARAVTDGIGPAVREMKALMDAARRGLRVTG"
     gene            3170..3559
                     /gene="acpS"
                     /locus_tag="PL152_07825"
     CDS             3170..3559
                     /gene="acpS"
                     /locus_tag="PL152_07825"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HQR49229.1"
                     /translation="MIFGIGTDVVQLARVEATYARHGEHFVRRLLMPAEETAFRSYRR
                     PVRFLAMRFAAKEAIVKALGTGFAHGVWIRDVGIVPNAWGRPEVIWSERGRRVRDGLG
                     AGEGHVTLTDEAGLVVAVAVLLRRGAT"
     gene            3556..4344
                     /locus_tag="PL152_07830"
     CDS             3556..4344
                     /locus_tag="PL152_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HQR49230.1"
                     /translation="MTISNVFAFDIETVPDVEFGRRLYGLEGLSDKQVGYVMQTRQRE
                     QTGSEFLSLEQQRVVAISVAMRTREGFKAWSLGDAASPESELVTRFYDGIARSSPTLV
                     SWNGSGFDLPVLHYRAMRHRVQAARYWETGDEEQSFRFNNYLGRFHWRHIDLMDVLAG
                     YQGRGRVGLDRMAQLLGLPGKLGMSGEGVWDAHLEGRVEDIRNYCETDVINTYLIYLR
                     FELMRGRLTPQEHDRECGIVRAWLQASGKPHLAEFLAAWPAAAE"
     gene            4341..>5580
                     /gene="rlmD"
                     /locus_tag="PL152_07835"
     CDS             4341..>5580
                     /gene="rlmD"
                     /locus_tag="PL152_07835"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: protein motif:HMM:NF009639.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HQR49231.1"
                     /translation="MKKISVAPEEADIVDLSHEAHGVARLDGKAVFVADALPGERVVL
                     RRVRRHRNFDEAVLEQVLSASPDRVPAECPHFGVCGGCALQHLAPAAQLAFKQGQLVE
                     NLARLGGVAPAALLPPLTGPTWGYRRRARLGIKYVPRKGRVLVGFRERSAPYVADLHE
                     CRVLAPPAGRLIDPLAALVAGLSIASRVPQAEVAVADDACALVLRVLDEPSATDLAAL
                     GRFEEDQRVRLYLQPGGADSVRPLRTGAPPLSYALPAFGLTLEFEPTDFVQVNGALNE
                     AMVSRAIEHLALDRGHRVLDLYCGLGNFSLPLATRAREVVGIEGDAALVHRAQRNASR
                     NLLANAQFHAANLAGDTTQATWARARYDRVLLDPPRAGAREVLPVVAASGAGRVVYIS
                     CHPGSLARDAGILVREHGFRL"
BASE COUNT          820 a         1885 c         2065 g          810 t
ORIGIN      
        1 gtctttctcc tgaccaagtt cgacaagctg gtcacgatcc agcagtaggc gggcttgctc
       61 acgtccggcg catggtccga ggcgcggctg ggataccgct tcggcgaccc ggcgctgctc
      121 gacgccgcgc tgacgcaccg cagcgccggc cgcgcgaatt acgagcgact cgagttcctg
      181 ggcgacgcgg tgctcaactt cgccgtggcc atgctcgtct accggaccta ccctgacgcg
      241 gacgaaggcg acctgagccg gtaccgcgcg agcctcgtga gcggcgagtc cctggcggac
      301 atcgctggcg gcctcggcct gggggagcag ctgcgcctcg gcagcggcga gctcaagacc
      361 ggcggattcc ggcgcaggtc gatcctggcc gacgcgctcg aggcgctgtt cggcgcgatt
      421 tacctcgacg gcgggcacac ggccgtggcg acggtgatcg agagcctgtt cgcgggccgt
      481 ctcgccgtgc tgccggaagc gcgggacctc aaggacccga agacgcgcct gcaggaactg
      541 ctgcagggaa aggctgagcc actgccgagc tatgcattgg aggacgtgag cggtgagcct
      601 cacgagcact ggttcgtcgc gagctgtgaa gtggcgtcgc tcggactgca agagcgaggc
      661 gaaggcacga gccggcggcg agccgagcag gccgccgcgc agcgcgtgct ggacctgctc
      721 gctgagcgag ggctcggcca atgaccgggc aggcgcaggc cggccacacg gcccgctgcg
      781 gctttgccgc gctggtcggc cggccgaacg tcggcaagtc cacgctgctg aatgcgctgc
      841 tcggccgcaa ggtgagcatc gtgagcccgc gaccgcagac gacgcgcacg cgcatccacg
      901 gcgtgctgac gcggccgggc atgcagatcg tgttcgtgga cctgcccggc atccacgcca
      961 agcagccgcg cgcgatcaac cgctacatga atcgcacggc gctggcgtcg ctcgccgatt
     1021 ccgacgtcaa cctgttcgtg atcgaggcgc tgcgctggac cgaggaggac gaacgcgcgc
     1081 tcgccgagtt gcgcgctgca gggcgtccga tactcctgct gatcaacaag tccgatcgtg
     1141 cccagccgcg gtcgcggctg ctgcccttca tcgcggaggt ctctgccaag gcggactttg
     1201 cgcaggtcgt gccgctgtcg gcgctcaagc gcaagaacct cgaccggttg ccggagttga
     1261 tcgcggggta cctgccggag tcgccgctgc tctttccgcc cgagcaggtg accgacgtga
     1321 gcgaccgctt catggccgcg gagatcgtcc gcgagaagct gacgcgacga ctgcaggagg
     1381 aagtgccgta cggcctcgtc gtggaggtcg aaggcatggg accggcggag gacgagccgg
     1441 gcaagctcgt gatccaggcg gtgatctggg tcgagcgggc cgggcagaag gcgatcgtga
     1501 tcggcaaggg cggcgagctg ctgaaggagg tcggccgtgc agcgcggctc gacctgaagc
     1561 actacttcgg caagccggtg cacctcgagc tctgggtcaa ggtgcgcgaa ggctggtccg
     1621 acgacgagag cgcgctgcgc cggttcgggt acgaagcgtg agcggcgcgg ctcgccgcgt
     1681 gcagctgcag cctgcctacc tgctgcacca ttacgcctgg cgggactcga gccggatcct
     1741 ggaactgctg acgcgcgacc atggacgcgt ctcggtattc gcgcgcgccg cccggagggg
     1801 aggctcgtcg ctgcccgcgg cattgcagcc gttctccgcg ctgctcgtgt cctggacggc
     1861 gcgcggggaa gccggccagc tcacgggagc ggaggcggcg gcgcccccgg cgcggatcga
     1921 cggggaccgg ctgatgagcg ccttctacgc caatgaactg ctggtcaagc tgttgccgcg
     1981 ccacgatccg cacccggcgc tctacgactc gtacgcgacc ctgctcgaga ggcttgccga
     2041 cgcgacgcgc cggccgtcgc gcgcgctgcg cctgttcgag aagcggctgc tcgacgaact
     2101 cggctggggc ctgagcctcg agtcggacgc ctcgggccgg ccgatcgacg tcggcggcag
     2161 ctaccgctat cgcgtcgacg gcggcgcgga acgactcgat ggagtcgcgg agggaagctt
     2221 ggtattttcc ggggcgtcgc tggtgtcgct ggcgcgggag gaactcggcg acgaccgcag
     2281 cctcgccgac gcgcgccggc tgctcaaggc ggcgctggac cagtgcctgg acggccggcc
     2341 actgcgcacg cgcgaagtga tgaacgcgat gcgcgcccgc ggtggcgcgc agggaggctg
     2401 aatcgtgctg acccatccgc tgaagctcgg cgtcaacatc gaccacgtgg cgacgctcag
     2461 gcaggtgcgc cgggcgattt acccggaccc gctctacgcg gcgctgatcg ccgagcagtc
     2521 cggcgccgac agcatcaccc tgcacctgcg cgaggaccgc cgccacatcc aggaccacga
     2581 cgtgacgcgc atgcgcgaga gcctgcagac gcgcatgaac ctcgagatgg cggcgaccga
     2641 ggcgatggtc cggttcgcgg ccgggctccg cccgcaggac tgctgcctgg tcccggagcg
     2701 ccgcgaggaa ctgacgaccg agggcggtct cgacgtcgcc ggccaggtcg accgcctgaa
     2761 ggattgctgc gcagctctcg acgacgcggg aatccgggtg tcgctgttca tcgatccgga
     2821 gcccgagcag ctcgaggcgg cgctcgatgc cggcgcgccg gtcgtcgagc tgcacaccgg
     2881 cgcgtatgcg gatgccacgg gcgagcgcca ggcgatggaa ctggcgcggc tcgtggaggc
     2941 ggcgcagttc ggcgcgcgga tcgggctcac ggtccatgcc ggacacgggc tcaactacca
     3001 caatgtccag ccggtggctg cgataccgga aatcgtcgaa ctcaacatcg gccacgcaat
     3061 cgtggcgagg gcggtgacgg acggcatcgg gcccgcggtg cgcgagatga aggcgctgat
     3121 ggatgcagcc cgtcgtggcc tcagggtcac gggctgaggc catgctcacg tgatcttcgg
     3181 catcggcact gacgtcgtgc aactggcgcg ggtcgaggcc acctacgccc ggcacggcga
     3241 gcacttcgtg cgccggctgc tcatgccagc ggaggagacc gcgtttcgct cgtaccgcag
     3301 gccggtgcgg ttcctggcga tgcgattcgc cgccaaggag gccatcgtca aggcactcgg
     3361 gaccgggttc gcgcacggcg tctggatccg cgacgtcggc atcgtcccga atgcgtgggg
     3421 ccgcccggag gtcatctggt ccgagcgggg ccgccgggtg cgcgacggcc tcggggcagg
     3481 cgaggggcac gtgacgctga cggacgaggc gggcctcgtg gtcgcggtcg ccgtgctcct
     3541 gcgacggggg gcgacgtgac catcagcaac gtgttcgcgt tcgacatcga gacggtgccc
     3601 gacgtcgagt tcggccgccg cctgtacggg ctcgagggat tgtccgacaa acaggtcggc
     3661 tacgtgatgc agacccgcca gcgcgagcag acgggatcgg agttcctctc gctcgagcag
     3721 cagcgcgtcg tcgcgatctc ggtcgccatg cggacgcggg agggcttcaa ggcctggagc
     3781 ctcggggacg ccgcctcgcc ggagtcggaa ctcgtgaccc ggttctacga cggtatcgcg
     3841 cgctccagcc cgacgctcgt gagctggaac ggcagcggct tcgacttgcc ggtgctgcac
     3901 taccgcgcca tgcggcaccg cgtgcaggcc gcccgctact gggagacggg cgacgaggag
     3961 cagtcgttcc ggttcaacaa ctacctgggg cgctttcact ggcggcacat cgacctgatg
     4021 gacgtgctgg ccggatacca gggtcgcgga cgcgtgggcc tcgaccgcat ggcccagctg
     4081 ctcggcctgc cgggcaagct cggcatgtcc ggcgagggcg tctgggacgc gcacctcgaa
     4141 gggcgggtgg aggacatccg caattactgc gagacggacg tcatcaacac ctacctcatc
     4201 tacctgcgct tcgaactgat gcgcggccgg ctcacgccgc aggaacacga ccgggagtgc
     4261 ggcatcgtgc gggcgtggct gcaggcgtcg ggcaagccgc acctggcgga gttcctggcc
     4321 gcctggcccg ccgctgctga atgaagaaga tcagcgtcgc gccggaagag gccgacatcg
     4381 tcgatctttc gcacgaagcc cacggcgtcg cgcgcctgga tggcaaggcg gtgttcgtcg
     4441 cggacgcgct gcccggggaa cgcgtcgtgc tgcggcgggt gcgccggcac cggaatttcg
     4501 acgaggccgt gctcgagcag gtgctgagcg cgagccccga ccgagtccct gccgagtgcc
     4561 cgcacttcgg cgtgtgcggg ggttgcgcgc tgcagcacct tgcgcccgcg gcccagctcg
     4621 cgttcaagca ggggcagctg gtcgagaacc tcgcgcgact cggcggcgtc gcacccgcgg
     4681 cgctattgcc gcccctgacg gggccgacgt ggggctaccg tcgccgcgcg cgcctgggca
     4741 tcaagtacgt gccgcgcaag ggacgcgtgc tggtcgggtt ccgcgagcgc tcggcgccct
     4801 atgtcgccga cctgcacgag tgcagggtgc tcgcgccgcc ggcgggccgg ctgatcgacc
     4861 cgctcgcagc gctggtcgcc gggctgtcga tcgcctcgag ggtgccgcag gcagaggtcg
     4921 cggtggccga cgacgcgtgc gcgctcgtgc tgcgcgtgct cgacgagccg tccgccacgg
     4981 acctggctgc gctcgggcgt ttcgaggagg accagcgggt gcgcctgtac ctgcagccgg
     5041 gtggcgccga ctccgtgcgc ccgctgcgca ccggcgcgcc tccgctgagt tacgcactgc
     5101 cggcgttcgg cctgacgctg gaattcgagc ccaccgactt cgtccaggtg aacggcgcgt
     5161 tgaacgaggc catggtcagc cgcgccatcg aacacctcgc cctggatcgc gggcaccgcg
     5221 tactcgatct ctactgcggc ctcggcaact tctcgctgcc gcttgcgacg cgcgcccggg
     5281 aagtcgtcgg catcgagggc gatgcggcgc tcgtccaccg ggcgcagcgg aatgcgtcgc
     5341 ggaacctgct cgcgaacgcg cagttccacg cggcgaacct ggccggggac acgactcagg
     5401 cgacgtgggc gagggcgcga tacgaccggg tgctgctcga cccgccgcgc gccggggcac
     5461 gcgaggtcct gcccgtggtg gctgccagcg gcgccggacg cgtcgtgtat atttcctgcc
     5521 acccgggcag cctggcccgg gacgccggga tcctcgtgcg ggaacacgga ttccggctgg
//
LOCUS       DAOMFD010000128         5496 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_5904_length_5496_cov_30.565153, whole genome shotgun sequence.
ACCESSION   DAOMFD010000128 DAOMFD010000000
VERSION     DAOMFD010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5496)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5496
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5904_length_5496_cov_30.565153"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..314
                     /locus_tag="PL152_07840"
     CDS             <1..314
                     /locus_tag="PL152_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQR49232.1"
                     /translation="EFQRCVEAEDVASLVRVPGIGRKTAERLVIEMRDRLRATAGPAA
                     AGMPGAMHGRAATPQAEAYSALVALGYKPAEVTRLLQKVDPAVTATEDLIRHALRAAA
                     G"
     gene            327..1370
                     /gene="ruvB"
                     /locus_tag="PL152_07845"
     CDS             327..1370
                     /gene="ruvB"
                     /locus_tag="PL152_07845"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HQR49233.1"
                     /translation="MIEQDRVISAGPQRDEEAYDRAVRPKSLAEYVGQPAMKAQMEIF
                     IEAARKRGDALDHVLIFGPPGLGKTTLAHVVANELGVGFKQTSGPVLERPGDLAALLT
                     NLQPNEVLFVDEIHRLSPVVEEILYPALEDFQLDIVIGEGPGARSIKIDLPPFTLVGA
                     TTRAGLLTSPLRDRFGIVQRLEFYGHEELRQIVDRSARILGIAMDDAGATRIAQRSRG
                     TPRIANRLLRRVRDYAEVKAQGRVTESVSQAALDLLDVDPQGFDPMDRRIMLTIIEKF
                     AGGPVGVDSLAAAVGEERGTIEDVIEPFLIQQGYIMRTARGRVATRNAYLHFGLTPPA
                     IEKQGGTLGLFDG"
     gene            1380..2057
                     /gene="tolQ"
                     /locus_tag="PL152_07850"
     CDS             1380..2057
                     /gene="tolQ"
                     /locus_tag="PL152_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HQR49234.1"
                     /translation="MIGEHSALDLILGASFVVQCVLALLVAASVMSWAIILRKRSVLA
                     EARDAAAAFEDKFWSGGDLASLFKSLDGSATRGMAGVFVAGFREFSRLRQQTGQGGGQ
                     LLEGSRRAMKVAQLRETDRLEQNLATLATIGSTSPYVGLFGTVWGIMSAFQGLGNVQQ
                     ATLATVAPAIAEALIATAMGLFAAIPAVIAYNRYADQVGRLELRYDTFMEEFSTILQR
                     HAGFGED"
     gene            2069..>2482
                     /gene="tolR"
                     /locus_tag="PL152_07855"
     CDS             2069..>2482
                     /gene="tolR"
                     /locus_tag="PL152_07855"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolR"
                     /protein_id="HQR49235.1"
                     /translation="MRNRRGRKLMGEINVVPYIDVMLVLLVIFMVTAPMLSQGIKVDL
                     PKAGAEPIQPDKLEPLVLSIDKDGQMYLNIGNPRAAQAPDRILEIASAAMRREPERPV
                     LVKADTAVAYGRVVEGMVLLQKSGARKVGFVTEPIP"
     assembly_gap    2483..2582
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2583..3035
                     /gene="tolA"
                     /locus_tag="PL152_07860"
     CDS             <2583..3035
                     /gene="tolA"
                     /locus_tag="PL152_07860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098567 - periplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HQR49236.1"
                     /translation="RRKAEAEAERKRAAEEAARQKQEAAARAAQDAKAKAAREADLQR
                     RMAAEEAEGAAMARAGVVDEYRAILVQAIERNWNRPPSARAGLSCTLYVTQATGGTVT
                     DVKLGTCNGDGAVRESIVNAVYKSSPLPAPRDPRAFERRLEIVFEPKE"
     gene            3043..4359
                     /gene="tolB"
                     /locus_tag="PL152_07865"
     CDS             3043..4359
                     /gene="tolB"
                     /locus_tag="PL152_07865"
                     /inference="COORDINATES: protein motif:HMM:TIGR02800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system beta propeller repeat protein
                     TolB"
                     /protein_id="HQR49237.1"
                     /translation="MRPGKVIKPSLAIARFATLAAALLGMAAPAVAQLRLDITEGVKD
                     AVPVAIVPFGGQPEGAATDIASVVAGDLRLSGRFAPLDRKDMVTRPTSGTEIRFEDWR
                     LLKADYIVVGHLDADGTSVSFELFNVQTGQPLLAEQVTAAGPNLRTAAHRISDLVFER
                     LTGIPGAFSTRIAYVSVEGRAPDRRYRLVVADADGYNPRTVAESGDPLMSPAWSPDGQ
                     SLAYVSFETKSSAIYVQKLATGERRRVSARSGINGAPAFSPDGTRLALTLSRDGNLDI
                     YVLELATQGLTRITTDDAIDTEAAWSPDGKTLYFTSDRAGNAQIYRVNLADPSSVQRV
                     TYTNGYNARPRLSPDGKSLAMVTLDRGYRIGLMDLGTRALQVLTDGRQDESPSFAPNG
                     AVIIYASEDRGPGSLAMVSSDGRYHERLSSSRGDVRDPAWSPYPPR"
     gene            4453..4986
                     /gene="pal"
                     /locus_tag="PL152_07870"
     CDS             4453..4986
                     /gene="pal"
                     /locus_tag="PL152_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR02802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HQR49238.1"
                     /translation="MKLTHLLAVAALSLGVVACQKPTQTKPAATPESQPAAEGATTSA
                     LPSSGAAAGSSTLTPQQQALAQIKSQSVIYFDYDSSEIPQQYLSAVSANASYLVKYPT
                     ARVRLEGNTDERGSREYNIGLGERRAQTVRRALLAQGVADAQITTVSYGEERPAVEGH
                     DETAYAKNRRVEFVYAQ"
     gene            4994..>5496
                     /locus_tag="PL152_07875"
     CDS             4994..>5496
                     /locus_tag="PL152_07875"
                     /inference="COORDINATES: protein motif:HMM:NF027655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070206 - protein trimerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbgF trimerization domain-containing protein"
                     /protein_id="HQR49239.1"
                     /translation="MSARISALAGAILLTCSAGAFATASNKELEARANDLDARLAAVE
                     RANQTLVQLQQQIESLRQELRSLRGQVDEVRHDLDGLRQQQRDLYTDLDRRLLLIENG
                     AAAGVPQGTQPIDAGRRDAASGAAGTSQPGPAAPSEDDIRAADETTLYSDSFAALKAG
                     RYDEAARG"
BASE COUNT          885 a         1833 c         1906 g          772 t
ORIGIN      
        1 acgaattcca gcgctgcgtg gaggccgagg acgtcgcgtc gctggttcgc gtgcccggca
       61 tcggccgcaa gaccgccgag cggctcgtca tcgagatgcg cgaccgtctc agggcgacgg
      121 cgggcccggc ggcggctggc atgcctgggg cgatgcacgg ccgggcggcc actccgcagg
      181 ccgaggccta cagcgcgctg gtcgcgctcg gctacaagcc cgcggaagtg acgcggctgc
      241 tgcagaaggt cgatccggcg gtcaccgcga ccgaggacct gatccgccac gcgctgcgcg
      301 cggcggccgg ctgacggagc aacgcgatga tcgagcagga ccgcgtgatt tccgccgggc
      361 cgcagcgcga cgaggaagcg tacgaccgtg ccgtgcggcc gaagtcgctc gcggaatacg
      421 tcgggcagcc cgccatgaag gcgcagatgg agatcttcat cgaggcggcg cgcaagcgcg
      481 gcgatgccct cgaccacgtg ctgatcttcg gtccgccggg gctcggcaag acgacgctgg
      541 cgcacgtggt ggcgaacgaa ctgggggtgg gcttcaagca gacctcgggc ccggtgctcg
      601 agcggccggg cgacctggct gcgctgctga ccaacctgca gcccaacgag gtcctgttcg
      661 tggacgagat ccaccggctg agcccggtcg tggaggagat cctctatccc gcgctcgagg
      721 acttccagct cgacatcgtg atcggcgagg ggccgggggc acgttcgatc aagatcgacc
      781 tgccgccgtt cacgctggtt ggcgccacga cacgcgccgg cctgctcacg tcgccattgc
      841 gcgaccggtt cggcatcgtg cagcggctgg agttctacgg ccacgaggag cttcggcaga
      901 tcgtcgaccg ctcggcgcgc atcctcggca tcgccatgga cgacgcaggc gccacgcgca
      961 tcgcgcagcg ctcgcgcggc acgccgcgca tcgcgaatcg gctcctgcgc cgcgtgcgcg
     1021 actatgccga ggtcaaggcg cagggacgcg taaccgagtc ggtgtcccag gctgcgctcg
     1081 acctgctcga cgtggatccc cagggcttcg acccgatgga ccgccggatc atgctcacga
     1141 tcatcgagaa gttcgcgggc ggcccggtcg gcgtggacag tctcgcggcc gcggtcggcg
     1201 aggagcgggg cacgatcgag gacgtgatcg agccgttcct gatccagcag ggttacatca
     1261 tgcgcaccgc gcgcggccgc gtggccacgc gcaatgccta cctccacttc ggcctgacgc
     1321 cgcctgcgat cgagaagcag ggcggtacgc tgggcctctt cgacggctga ggatgcagga
     1381 tgatcggtga acactccgcg ctggacctga tcctcggcgc cagcttcgtc gtgcagtgcg
     1441 tgctcgcgct gctcgtggcc gcctcggtga tgtcctgggc catcatcctg cgcaagcgca
     1501 gcgtactcgc cgaggcgcgc gatgcggccg ccgcattcga ggacaagttc tggtcgggag
     1561 gcgacctcgc gtcgctgttc aagtcgctcg acggcagcgc gacccggggc atggccggcg
     1621 tgttcgtcgc gggtttccgc gaattctcgc gcctgcggca gcagaccggt cagggcggag
     1681 gacagttgct cgagggcagt cgccgcgcga tgaaggtcgc gcaactccgc gagaccgatc
     1741 gcctcgagca gaaccttgcg acgctcgcga ccatcggctc gacgagcccg tacgtgggcc
     1801 tgttcgggac ggtgtggggc atcatgagcg cgttccaggg gctcggaaac gtgcagcagg
     1861 cgacgctcgc gacggtggcg ccggcgatcg ctgaggcgct gatcgccacc gcgatgggcc
     1921 tgttcgcggc gatccctgcg gtgatcgcct acaaccgcta tgccgaccag gtcgggcgcc
     1981 tggaactgcg ctacgacacg ttcatggagg agttctccac catcctgcag cgccacgccg
     2041 gtttcggcga ggactgagga gcgcgggcat gcgcaaccgt cgcggccgga agctgatggg
     2101 tgagatcaac gtcgtaccgt acatcgacgt gatgctggtg ctgctggtca tcttcatggt
     2161 gaccgcgccc atgctgagcc agggcatcaa ggtggacctg ccgaaggccg gcgccgagcc
     2221 gatccagcca gacaagctcg agccactggt gctcagcatc gacaaggacg ggcagatgta
     2281 cctgaacatc ggcaatccac gcgctgcgca ggccccggat cggatcctcg agatcgcatc
     2341 cgccgcgatg cgcagggaac ccgaacgccc cgtgctggtc aaggctgata cggccgttgc
     2401 gtacggtcgc gtcgtggagg gcatggtgct gttgcagaag tccggtgcca ggaaggtcgg
     2461 cttcgtcacc gagccgatcc cgnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nncgccggaa ggcggaggcc gaggcggagc gcaagcgggc tgcggaggag gccgctcgac
     2641 agaagcagga agcggccgcc cgggccgcgc aggatgccaa ggcgaaggcg gcacgcgagg
     2701 cggacctgca acggcgcatg gcagcggagg aagcggaagg tgccgcgatg gcgcgcgccg
     2761 gcgtcgtcga cgagtatcgc gccatactgg tgcaggcgat cgagcgcaac tggaaccgcc
     2821 cgccttcggc gcgggccggg ctctcgtgca cgctgtacgt gacgcaggcg acgggtggca
     2881 cggtgacgga tgtcaagctc ggcacctgca acggcgacgg cgccgtgcgg gaatccatcg
     2941 tcaatgccgt gtataaatcc tcgccgctgc cggcaccgcg cgacccacgc gcgttcgagc
     3001 gcaggctcga gatcgtgttc gagccgaagg agtagtcatt cgatgcgtcc tggaaaggtg
     3061 atcaagccat cgcttgcgat cgcacgattc gcaactctcg ccgcggctct cctgggcatg
     3121 gcggcgccgg cggtcgcgca actgcgcctc gacatcaccg aaggcgtgaa ggacgcggtc
     3181 ccggtcgcga tcgtgccgtt cggcgggcag cccgagggcg ctgcgacgga catcgcgagc
     3241 gtggtcgccg gcgacctgcg cctgagcgga cggttcgcgc ccctcgatcg caaggacatg
     3301 gtgacccggc cgaccagcgg caccgagatc cgcttcgagg actggcggtt gctcaaggcc
     3361 gattacatcg tcgtcgggca cctggatgcc gacggcacct cggtgtcgtt cgagctattc
     3421 aacgtgcaga ccggccaacc gctgctggcc gagcaagtca ccgcggccgg cccgaacctc
     3481 cgcaccgcgg cgcaccggat ctccgacctc gtgttcgagc gcctgaccgg cattcccggc
     3541 gcattctcga cgcggatcgc ctacgtctcg gtggagggtc gggcgcccga tcggcgctac
     3601 cgactcgtgg tcgcggatgc ggacggctac aacccgcgca ccgtggcgga atcgggcgac
     3661 ccgttgatgt cacctgcgtg gtcgccggac ggccagagcc tggcctacgt ctccttcgag
     3721 accaagtcgt cggccattta cgtgcagaag cttgcgacgg gagagcgacg ccgcgtgtcg
     3781 gcacggtccg gcatcaatgg cgcgccggcg ttttcgccgg acggtacgcg gctcgcgctc
     3841 acgctgtcgc gcgacggcaa cctcgacatc tacgtgctgg agctggccac ccaggggctg
     3901 acgcgcatca cgaccgacga cgcgatcgac accgaggccg cgtggtctcc ggacggcaag
     3961 accctgtact tcacgtccga ccgggcggga aacgcacaga tctatcgcgt gaacctcgcc
     4021 gacccgtcga gcgtgcagcg cgtcacgtac acgaacgggt acaacgcacg gccccgcctg
     4081 tcgcccgacg gcaagtcgct cgcgatggtg acgctcgacc gtggctaccg catcggcctg
     4141 atggacctcg ggactcgcgc gctgcaggtg ctcaccgacg ggcgccagga cgagtcgccg
     4201 agcttcgcgc cgaacggcgc cgtcatcatc tatgcgagcg aggaccgcgg tccgggatcg
     4261 ctcgccatgg tgtcgagcga cggtcgctac catgagcgac tgagctcgag ccgtggcgac
     4321 gtgcgggacc cggcgtggtc accgtatccg ccgcgctgat gaacgtgccg cgtcgttgtg
     4381 gtaaaaaccc gcgcaacctc tagcgggcac atccgcccgc gtcccgtgac cgatcctcga
     4441 ggagcctgcc agatgaagct gacacacctg ctcgccgtgg ccgcattgtc cctgggcgtg
     4501 gtcgcctgcc agaagcccac gcagaccaag ccagcggcga cgcccgaatc gcagccggcg
     4561 gctgaaggcg ccacgaccag cgcgctgccg tcgtccggcg ccgccgccgg cagttccacc
     4621 ctgaccccgc agcagcaggc cctggcccag atcaagagcc agagcgtcat ctatttcgac
     4681 tacgacagca gcgagatccc gcagcagtac ctgtcggccg tctcggccaa tgcgagctac
     4741 ctcgtcaagt acccgaccgc ccgggtgcgc ctcgagggca acaccgacga gcgaggctcg
     4801 cgtgagtaca acatcgggct cggtgaacgc cgtgcgcaga cggtgcgcag ggcgctgctc
     4861 gcgcagggcg tggcggacgc gcagatcacc accgtgagct acggtgagga gcggccggcg
     4921 gtcgaagggc acgacgagac ggcgtacgcg aagaatcgcc gcgtcgagtt cgtgtacgcg
     4981 cagtgattcc gtcatgagtg cacgcatcag cgccctggcg ggtgcaatcc tgctcacctg
     5041 cagcgccggg gcgttcgcga ccgccagcaa caaggaactc gaggctcgcg caaacgacct
     5101 ggatgcgcgg ctcgcggcgg tcgagcgcgc caaccagacg ctcgtgcagc tgcagcagca
     5161 gatcgagtcg ctccgccagg aactgcgttc cctgcgcgga caggtggatg aggtgcgcca
     5221 cgacctcgac ggccttcgcc agcagcagcg ggatctctat acggacctcg accgtcgcct
     5281 gctgctcatc gagaacggcg ccgcggccgg ggttccccag ggcacgcagc cgatcgacgc
     5341 gggccggcgc gacgcggcgt ccggcgcagc cgggacctcg cagccggggc ccgctgcgcc
     5401 gtccgaagac gatattcgcg ccgccgacga gacgacgctc tacagcgact cgttcgcggc
     5461 gctgaaggcg ggacgctacg acgaggcggc cagggg
//
LOCUS       DAOMFD010000129         5415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6075_length_5415_cov_37.069403, whole genome shotgun sequence.
ACCESSION   DAOMFD010000129 DAOMFD010000000
VERSION     DAOMFD010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5415
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6075_length_5415_cov_37.069403"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            30..680
                     /locus_tag="PL152_07880"
     CDS             30..680
                     /locus_tag="PL152_07880"
                     /inference="COORDINATES: protein motif:HMM:NF016207.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HQR49240.1"
                     /translation="MYIPGAFAVTDAARITGFVDSHPLAILVGQVDGRLQANHLPFIR
                     ASELAIGEYLIAHTARANPVWRLGEAGANVLLVFSGADAYVSPSFYPSKATDPRTVPT
                     YNYVAVHVAGTLSCSHERNDKRDAVTLLTRRMEAGRPDPWAIDDAPVDYIDRMLDGIV
                     ALRLRIESIEAKWKASQNRTEVDRRGVIEALRAAVHDSPAVEAARAAEHGLEPRNR"
     gene            768..3422
                     /locus_tag="PL152_07885"
     CDS             768..3422
                     /locus_tag="PL152_07885"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF023266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HQR49241.1"
                     /translation="MLRHLLLPAVAGLMLLACSASAAPSVPTGRLPDDVQPQRYRLQL
                     DIDPRQARFSGATDIDIDVKRPVRTIWLHGHGLDVRKVTVESGGRLLNARYKEIEPLH
                     GVAKIDLPRPLPVGRATLHFEYTAPFRDSPDGLYRTKSGEDWYAFTQFESIDARRAFP
                     CFDEPRFKTPFEVTVTAPASDKVVTNGPDADATVIADGRMRHRFEPTLPLPTYLVAFA
                     VGPLDIVGIKVPPNSVRSKELSLRAVATRGREPQLAYAIKHTPEIIAALEQYFAIPYP
                     YPKLDIIASPVHLGAMENAGAIIFDDTYVLLDEPARPSQERNFFEVVAHEVAHHWFGD
                     LVTPVWWDDIWLNESFAEWMGVKIAAQLRPDLGSRAVESAGVVYAMDVDSKRAGRPIH
                     EPVTDNSRIDSTFDAITYLKGGQVLAMVESYVGAEKFRSGIQDFLKRHEHGSATSDEF
                     FAALAQASGQPAVIAAFRSFVDQPGVPLISIATGPGNDAELVVSQDRYLPIGSTLTGG
                     QRWQVPLCMHQYPGSGAPSKTCSLLADRSGSYRVATGTVALMPNADGAGYYRFALPGP
                     RFNALLDAAPRLPESESIMVADSAAAAFLAGRLEFPQLLDAASRLSTSPNSVAATLLG
                     DELAMIKDRWADAATRDAMAARIRSIYGPRLAGLGLDPRRGAYSAEPADRRDLRRLLA
                     TLVIDEGRDADLRRKLAETARASLDDPAAIDPEFRPLVWTVGVQDLGESFGQALEARM
                     LASDDGLLRADAAWALGNAEDSQGSRRALALVLRPDVRPGEVGRILYPQFASPLTRDS
                     AWAWFKSNTDRVMDKVASIFQARYFELLDSFCDSPHRNDIAQSLEAQVRKLGSGELEM
                     QRTLEGIDLCVAQRTALGPSVEAAMRTQ"
     gene            3443..>5415
                     /locus_tag="PL152_07890"
     CDS             3443..>5415
                     /locus_tag="PL152_07890"
                     /inference="COORDINATES: protein motif:HMM:NF014896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HQR49242.1"
                     /translation="MDSRSRTPIAPPIARVEPATDAYFGEKVTDPYRWMENDQDRDWR
                     PYLEAQNAHTRAVLDNLPGRDALLERIQALSGALPTVAEVQLAGRCLFYEQRPAGANN
                     FKLFVRESGQDRLLIDPTTYDTDGHHVSLDWWEAAPDGSKVAFGLSQDGSEDSTLHVV
                     ETATGRVLPERIPQAEGANPQWVPDGTGFFYNQLTGAVGSPDRFLDSRARFHRLGTDP
                     AADPVVMARGLDPRVVFDRIQMPYVYTFTTSADVILVLADVRTEARLFIANRADVLAG
                     QARWRPVADFPDEITDFDLDGNVLYLLANRGHPRGRVLKVATQSPDLAGAVEVVPESA
                     LVLEDLNRAQDGLYIRAMDGGLGRLLRFGNDGKLSGIALPFDGTLGDIATDVDAPGAV
                     LILSGWLTPTGVWTVDARGKVSDTGLTSKPSIDVSPYATERRFATARDGTRIPYDLVY
                     RKDLERDGSNPAYISAYGSYGIPANTPRYLGRSLALVDQGAIVGYASVRGGGEYGRDW
                     HRGGQLDNKPNTWRDLIAVCEDMIANGYTAPSHLAIGGRSAGGITVGRAMTERPDLFA
                     AVVSGVGWHNPLRYVAEQNGYGEEPEWGAISTESGYRALRRIDSYHAVEDGANYPAVL
                     LTTGVTDPRVSPFHAAKMAARLQAASASGRPILL"
BASE COUNT          880 a         1849 c         1821 g          865 t
ORIGIN      
        1 cccgcacctg accaggagcc gcgcctgcca tgtacatacc gggagcgttt gcggtcacgg
       61 atgcagcacg gatcaccggg ttcgtcgact cgcatccgct cgcgatcctc gtcgggcagg
      121 tcgacggccg gttgcaggcc aatcacctgc cattcatacg cgcgtcggaa ctggcgatcg
      181 gcgagtacct cattgcacac acggcccgcg cgaatcccgt gtggcgactg ggcgaggccg
      241 gcgcgaacgt cctgctcgtg ttctccggtg cggacgccta cgtatctcca tcgttctatc
      301 cgagcaaggc gaccgaccca aggaccgtcc cgacctacaa ctacgtcgcg gtgcacgtcg
      361 ccggcacgct gtcgtgctcg cacgagcgca acgacaagcg ggacgcagtg acgctgctga
      421 ctcgccggat ggaagccggc cggcccgacc cctgggcgat cgacgacgcg ccggtcgact
      481 acatcgaccg gatgctcgac ggcatcgtcg cgctcaggct gcgcatcgaa tcgatcgagg
      541 cgaagtggaa ggccagccag aatcgcaccg aggtcgaccg ccgcggcgtg atcgaggcgc
      601 tccgtgctgc agtgcacgat tcgccagcag tcgaggcggc ccgggccgcc gagcacggcc
      661 tcgaaccccg gaatcgttga tgtcggcatg ccgggggcgg tagtgtccgc cgacgggctt
      721 cgcgctggtc gtcggattcg gatacttcgc acaaggatag tcgagccatg cttcgtcacc
      781 tgctgctgcc ggcggtcgcc ggcctgatgc tcctcgcgtg cagcgcgtcc gccgcgccgt
      841 ccgttcccac cggccggctg ccggacgacg tgcagccgca gcggtaccgc ctgcagctcg
      901 acatcgatcc tcgccaggcg cggttttccg gagccacgga catcgacatc gacgtgaagc
      961 gtccggtgcg cactatctgg ctgcacggcc atggcctcga cgtgcgcaag gtcacggtcg
     1021 agagcggagg acgactgctg aatgcccggt acaaggaaat cgagcccctg cacggagtcg
     1081 cgaagataga cctccctcgc ccgctccccg tcggtcgagc cacgctgcac ttcgaataca
     1141 ccgcgccgtt ccgggacagt cccgatggac tgtatcggac caagtctggc gaggactggt
     1201 atgcattcac gcagttcgag tcgatcgacg cgcgccgggc attcccctgt ttcgacgagc
     1261 cgcgattcaa gaccccattc gaggtgaccg tcacggcgcc ggcgtcggac aaggtcgtga
     1321 cgaatggacc ggacgccgac gcaacggtga tcgccgacgg ccgcatgcgg caccggttcg
     1381 agccgacctt gcctttgccg acctacctgg ttgcgtttgc cgtcggtccg ctcgacatcg
     1441 tcggtatcaa ggtcccgccc aattccgtgc gcagcaagga gctgtcgctg cgagccgtgg
     1501 cgacccgcgg ccgcgagccg caactcgcct acgcgatcaa gcacacgcca gagatcattg
     1561 cggcgctgga gcagtacttc gcaattccct atccgtaccc caagctggac atcatcgcgt
     1621 cgccggtgca cctcggtgcg atggagaacg ccggcgcgat catcttcgac gacacctacg
     1681 tgctgctgga cgagcccgcg cgaccctcgc aggagcgcaa cttcttcgag gtcgtggcgc
     1741 acgaggtggc ccatcactgg ttcggagacc tggtcacgcc ggtgtggtgg gacgacatct
     1801 ggctcaacga gtcgtttgcc gagtggatgg gcgtgaagat tgccgcccag ctgcgacccg
     1861 acctcggcag ccgcgccgtc gagtccgccg gcgtggtgta tgcgatggac gtggactcga
     1921 agcgcgccgg ccggccgatc cacgaaccgg tgacggacaa ctcccggatc gacagcacgt
     1981 tcgacgcaat cacgtacctg aagggcggcc aggtgctggc catggtcgag tcgtatgtcg
     2041 gcgcggagaa attccgcagc ggcatccagg atttcctgaa gcgccacgaa catgggtcgg
     2101 cgacctccga cgaattcttt gcggcgctgg cgcaggcgtc cggccagccg gccgtgatcg
     2161 ccgcgttccg gtcgttcgtg gaccagccgg gagtaccgct gatttcaatc gctacgggcc
     2221 cgggcaatga cgccgagctg gtcgtcagcc aggatcgcta tctcccgatc ggcagtacgc
     2281 tgaccggcgg ccagcgctgg caggtgccgc tgtgcatgca tcaatatccg ggctcgggcg
     2341 caccgagcaa gacctgttcg ctgcttgcag accgttcggg ctcatatcga gtggcgaccg
     2401 gcacggtcgc gctcatgccg aatgcggatg gcgccggtta ctaccgcttt gcactgcccg
     2461 ggccgcggtt caacgcgctg ctggatgcgg caccgaggtt acccgagtcg gaatccatca
     2521 tggtcgccga cagcgcggcc gctgcattcc tcgccgggcg cctcgaattc ccgcagttgc
     2581 tggacgccgc gtcacggctg tcgaccagcc cgaacagcgt tgcggcgacg ttgctcggcg
     2641 acgagctcgc gatgatcaag gatcgctggg cggacgccgc cacgcgtgac gcgatggctg
     2701 cacgcatccg ttcgatctac ggaccgcgcc tcgcagggtt ggggctcgat ccccgacgcg
     2761 gcgcgtattc ggcggaaccc gcggatcgcc gcgacctgcg caggctgctc gcgacgctcg
     2821 tgatcgacga gggacgcgac gccgacctgc gccgcaaact tgcggagacc gcgcgggcct
     2881 ccctcgacga cccggctgcg atcgatccgg agttccggcc gctggtctgg accgtcggcg
     2941 tgcaggacct gggcgagtcg ttcggccagg ccctcgaggc gaggatgctt gcgtccgatg
     3001 acggcctgct gcgagccgac gccgcatggg cactcggcaa tgccgaggac agccagggtt
     3061 ctcgacgcgc gctggcgctg gtactgcgac cggacgtccg gcccggcgag gtcggcagga
     3121 tcctgtaccc gcagttcgcg tcgccactga cgcgcgactc ggcgtgggcc tggttcaagt
     3181 cgaatacgga tcgagtcatg gacaaggtgg cctcgatctt ccaggcgcgt tacttcgagc
     3241 tcctggattc tttctgcgat tcgccccatc ggaacgacat cgcccagtcg ctggaagcac
     3301 aggtccggaa gctgggcagc ggcgagctcg agatgcagcg cacgctcgag ggcatcgacc
     3361 tctgcgtcgc gcagcggact gcgctcggcc cgtccgtcga ggcggcaatg cgcacccagt
     3421 aagtcacgag cggcgcgtgc acatggattc ccgatcccga acgcccatcg cgccgccgat
     3481 tgcccgggtc gagccggcga ccgacgccta tttcggcgag aaggtgacgg acccgtaccg
     3541 gtggatggag aacgaccagg accgcgactg gcggccgtac ctcgaagcgc agaacgctca
     3601 cacccgcgcg gtgctggaca atctgcctgg acgtgacgcg ctgctcgaac gcatccaggc
     3661 actgtcgggt gcactcccga cggtcgccga ggtccagctc gccggccgct gcctgttcta
     3721 cgagcagcgc cccgctggcg cgaacaactt caagctgttc gtgcgcgagt cggggcagga
     3781 ccggctgctc atcgatccga cgacgtacga caccgacggt caccacgttt cgctcgactg
     3841 gtgggaggcg gcgcccgacg gcagcaaggt cgccttcgga ctgtcgcagg acggcagcga
     3901 ggactcgacg ctgcatgtcg tggagaccgc caccgggcgc gtgctgcccg agcggatacc
     3961 gcaggcggaa ggcgccaatc cgcagtgggt tccggatggg acgggcttct tctacaacca
     4021 gctcacgggc gcggtcggca gtcccgatcg cttcctggac tcgcgcgcgc ggtttcatcg
     4081 tctcggtact gatcccgccg ctgatccggt cgtcatggcg cgcgggctgg atccgcgcgt
     4141 ggtcttcgac cggatccaga tgccgtacgt gtacacgttc actacgtctg cggacgtgat
     4201 cctcgtgctg gcggacgtgc gtaccgaagc gcgcctgttc atcgcgaatc gcgcggacgt
     4261 cctggcaggc caggcccgct ggcgacccgt cgccgacttc cccgacgaga tcacggactt
     4321 cgacctggac ggcaacgttc tctacctgct ggcgaaccgc ggccatccgc gcggccgcgt
     4381 gctcaaggtc gcgacgcaat cgccggatct cgccggagcc gtcgaagtcg tgccggagtc
     4441 cgccctggtg ctcgaggacc tgaatcgcgc gcaggacgga ctctacatcc gcgccatgga
     4501 cggcggactc ggtcgcctgc tgcggttcgg gaatgacggc aagctctccg gcatcgcctt
     4561 gcctttcgac ggcacgctcg gcgacatcgc gacggacgtg gacgcgcctg gcgcggtgct
     4621 gatcctgtcg gggtggctca cgccgaccgg ggtctggacc gtcgacgccc gcggcaaagt
     4681 gagcgacacc ggactcactt ccaagccgtc gatcgatgtc agcccttacg cgacggaacg
     4741 ccgcttcgcg acagccaggg acggcacgcg cattccgtac gacctggtct atcgcaagga
     4801 cctcgagcgc gacgggagca acccggccta catctccgcg tacggcagct acggcatccc
     4861 agccaatacg ccgcgttacc tgggaagatc tctggcgctg gtcgaccagg gcgcgatcgt
     4921 gggctacgcg agcgtgcgcg gcggcgggga atacgggcgt gactggcatc gcggcggcca
     4981 gctcgacaac aagccgaaca cctggcgcga cctgatcgcc gtgtgcgagg acatgatcgc
     5041 gaacggttac accgcgccgt cgcaccttgc gatcggaggt cgctccgccg gaggcatcac
     5101 ggtcggccgt gcgatgacgg agcgtcccga cctgttcgcg gccgtcgtgt cgggcgtggg
     5161 ctggcacaac ccgctgcgct acgtcgcgga gcagaacggt tacggcgagg agcccgagtg
     5221 gggcgcgatc agcaccgaga gtggctatcg cgcgctcagg cggatcgaca gctaccacgc
     5281 ggtggaggac ggcgcgaact accctgccgt gctgctgacc acgggcgtga ccgatccgcg
     5341 cgtgtcgcca ttccacgctg ccaagatggc ggcgcgcctg caggccgcga gcgccagcgg
     5401 caggccgatc ctgct
//
LOCUS       DAOMFD010000130         5341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6215_length_5341_cov_32.683504, whole genome shotgun sequence.
ACCESSION   DAOMFD010000130 DAOMFD010000000
VERSION     DAOMFD010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5341
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6215_length_5341_cov_32.683504"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /locus_tag="PL152_07895"
     CDS             complement(<1..1316)
                     /locus_tag="PL152_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HQR49243.1"
                     /translation="MADYKHTINLPETAFPMKGDLARREPEMLAWWEDHRTYATLRAI
                     AADRPRYVLHDGPPYANGAIHIGHAVNKVLKDIIVKARGLDGYDAPYVPGWDCHGLPI
                     EHAVEKSRGRDAKVLDAKAFRQACREFAMEQVDVQRKDFIRLGVMGEWGNPYLTMAPR
                     YEAEQLRAFAQIIRNGHLYKGYKPVHWCLDCRSALAEAEVEYEERTSPSIDVRFEVVD
                     RADLARRFGAGSVPDRRASVVIWTTTPWTLPANHAVALHPEFEYSLLEVESGGGTDML
                     VLASELAAAVLSRAGAKSLREIARAKGAALEGLQLRHPFYERIVPVILGDHVTLDSGT
                     GAVHTAPGHGQEDFVVGRKYGLPVDNPVGADGRFLPSTPVFAGQKVFDANKHVVELLA
                     EKGALLHQESVRHSYPHCWRHKTPVIFRATPQWFISMEQAGLRAKAL"
     gene            complement(1418..2344)
                     /locus_tag="PL152_07900"
     CDS             complement(1418..2344)
                     /locus_tag="PL152_07900"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HQR49244.1"
                     /translation="MELVRGLHNLRESHRGSVVTIGNYDGVHRGHQHMLAAVRERAVQ
                     LGLPATVVTFEPTPREFFEGPAAPARLMRLREKIEALPLYGVDRVVVLRFDANMRAMS
                     ASEFVDRLLVSGLGTRHVVVGHDFHFARRREGTVATLREAGVRHGFEVQQVGRFLVDG
                     ERVSSSLVRDALERGDLDRASLLLGRPYRMAGRVRRGQQLGRKLGFPTANLALHRKVV
                     PLWGVFAVRVSGPGLVNHPAVVSLGTRPTINGTEPLLEVHVFEFDGDLYGKYLDVDFV
                     RRLRDERRFESLDALVEQMHRDAAEARAALGA"
     gene            complement(2404..4002)
                     /gene="murJ"
                     /locus_tag="PL152_07905"
     CDS             complement(2404..4002)
                     /gene="murJ"
                     /locus_tag="PL152_07905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HQR49245.1"
                     /translation="MSGFFRSTSIVGGLTLLSRVTGLLRDMVYSRMFGAGVLMDAFLV
                     AFKIPNFMRRLFAEGAFSQAFVPVVSEYKVQRTHDEVRDLVDGAMGTMAWFMSVVTIV
                     GILAAPVFVLLFAPGFHADAGKFELTVDMLRWTFPYLLFISLASLASGVLNSYQRFSV
                     PALMSTLMNLVMIAFAAWIAPRFPKPGVVLAVGVFVAGAVQLAFQLPSMVRLGLLRRP
                     RWQWHHPGVRKIGQLMLPAIFGSSVSQVALLLDTLIASFLVTGSISWLYYADRLMEFP
                     LGVFSVALATVILPGLAAHHAAESIERFAATLDWALRLTIIVVAPATVALIVLAGPLT
                     VAIFHYGEFNAEDVRMSMLAVMAFATGLMGFSLVKVLAPGFFARQDTRTPMKIGIRSL
                     VVNMALNVFVVLPLALYGNRPGLHALLALNIGISAWYNAAQLYRGLRRAHVLHHAPGW
                     RAMLIQVLVANVVMGLFLWFVAGDTSDWMAMRTMKRMEWTLVLVAGGATLYFATLYVL
                     GMRIGHLRVRSVGPIPPKPEGPAQ"
     gene            4229..4498
                     /gene="rpsT"
                     /locus_tag="PL152_07910"
     CDS             4229..4498
                     /gene="rpsT"
                     /locus_tag="PL152_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HQR49246.1"
                     /translation="MANIKSAEKRAKQAVKRRAHNMAARSRLRSSIKKVLAAVAAGDK
                     AGASAAFKAAQPVIDTMVTKGIIHANKASRHKRELNAKVKALAAK"
     gene            complement(4574..>5341)
                     /gene="obgE"
                     /locus_tag="PL152_07915"
     CDS             complement(4574..>5341)
                     /gene="obgE"
                     /locus_tag="PL152_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HQR49247.1"
                     /translation="VGTVVYDVDTEEVLGDLAHGGDRVLVAKGGKGGLGNQHFKSSTN
                     RTPRKATPGFPGEKRELRLELKLIADVGLLGLPNAGKSTLISAVSAARPRIADYPFTT
                     LHPNLGVVYVGEHKSFVMADIPGLIEGAAEGAGLGIRFLRHLQRTRLLLHLVDIAPPD
                     PDADPVKDARAIAAELKKFSPELAARERWLVLNKLDLLPADAADKRCREIVRRLRWKG
                     PVFRISGATRAGTRELCQAVMRRLDEIAADARSASAE"
BASE COUNT          904 a         1838 c         1810 g          789 t
ORIGIN      
        1 agcgccttcg cacgcagtcc cgcctgctcc atgctgatga accactgcgg cgtcgcgcgg
       61 aagatcaccg gcgtcttgtg gcgccagcag tgcgggtagc tgtggcggac cgattcctgg
      121 tgcagcagcg cgcccttctc ggccagcagc tcgacgacgt gcttgttcgc gtcgaagacc
      181 ttctggcccg cgaagaccgg cgtcgacggc aggaaccgcc cgtccgcgcc gacgggattg
      241 tcgaccggca gcccgtactt gcggcccacc acaaagtctt cctggccgtg gccgggcgcc
      301 gtgtgcaccg cgccggtacc gctgtcgagc gtcacgtggt cgccgaggat tacgggcacg
      361 atccgctcgt agaacgggtg acgcagttgc agtccttcga gcgccgcacc tttcgcgcga
      421 gcgatttcgc gcagtgactt cgcgccggcg cgcgacagta cggcggcggc gagctcggat
      481 gccaatacga gcatgtcggt cccgccgccg gattccactt cgagcagtga atactcgaat
      541 tccgggtgca gtgcgacggc gtggttcgcc ggcagcgtcc acggcgtcgt ggtccagatg
      601 acgacgctcg ccctgcggtc ggggacggat ccggctccga agcgtcgcgc gaggtccgcc
      661 cggtccacga cttcgaatcg gacgtcgatg gacggcgacg tgcgttcctc gtactccacc
      721 tcggcctcgg ccagcgccga gcggcagtcg aggcaccagt gcacgggctt gtagcccttg
      781 tacaggtgcc cgttgcggat gatctgcgcg aacgcacgca actgctcggc ctcgtagcgc
      841 ggggccatcg tcaggtacgg gttcccccac tcgcccatga cgccgaggcg gatgaagtcc
      901 ttgcgctgga cgtcgacctg ttccatcgcg aactcgcgac aggcctggcg gaatgccttg
      961 gcgtcgagca ccttggcgtc gcgcccgcgt gacttctcga ccgcgtgctc gatgggcagc
     1021 ccgtgacagt cccagcccgg cacgtaaggc gcgtcgtagc cgtcgagccc gcgcgccttg
     1081 acgatgatgt ccttcaggac cttgttgacc gcgtggccga tgtggatcgc gccgttcgcg
     1141 tagggcggac cgtcgtggag gacgtacctg gggcggtccg ccgcgatggc ccgcagcgtc
     1201 gcgtaggttc gatgatcctc ccaccacgcg agcatctccg gctcgcggcg agcgaggtcg
     1261 cccttcatcg ggaaggcggt ctcggggagg ttgatggtgt gcttgtagtc agccatgcga
     1321 aggacgcgtc aggacccggg ggcggcgagc aaagaactgg gaattttgcc gtaacagcgg
     1381 cattttctcc attgaaatcg gcgacttgcc cgcggactca ggcgccgagc gcggcacggg
     1441 cttccgcggc gtcccgatgc atctgctcga ccagcgcgtc gagcgactcg aaccgccgct
     1501 cgtcccggag ccgtcggacg aaatccacgt cgaggtactt cccgtagagg tcgccgtcga
     1561 actcgaagac gtgcacctcg aggagcggtt ccgtgccgtt gatcgtggga cgcgtgccga
     1621 ggctcaccac cgcgggatgg ttcacgaggc ccggaccgct cacgcgcacc gcgaacacgc
     1681 cccacagggg cacgaccttc cggtgcagtg caaggttcgc agtcgggaag ccgagcttgc
     1741 ggccgagttg ctggccgcgc cggacgcgac cggccatccg gtacgggcga ccgagcagca
     1801 ggctggcacg atcgaggtcg ccccgctcca gcgcgtcgcg caccagcgag ctcgagactc
     1861 gctcgccgtc gacgaggaaa cgtccgacct gctgcacctc gaatccgtgg cgcacgccgg
     1921 cctcgcgcag cgtggccacc gttccctcgc gcctgcgggc gaagtgaaaa tcgtggccca
     1981 cgaccacgtg gcgcgtgccg aggccgctca cgagcagcct gtcgacgaac tccgacgcgc
     2041 tcatggcgcg catgttcgcg tcgaagcgca gcacgacgac gcggtcgacc ccgtagagcg
     2101 gcagcgcctc gatcttctcg cggagccgca tcaggcgcgc cggcgccgct gggccttcga
     2161 agaattcgcg cggcgtgggc tcgaacgtga cgaccgtcgc cgggagcccg agttgcacgg
     2221 cgcgctcgcg aacggccgcg agcatgtgct ggtggccgcg gtgcacgcca tcgtagttgc
     2281 cgatggtcac cacggagccg cgatgcgact cgcgcaggtt gtgcagtccg cgcacgagtt
     2341 ccatggccgg ctagttccag gcgagagtgg gcgcacgcca cgcgcgatcg gccaccgccg
     2401 cactcactgc gccgggccct cgggcttcgg cgggatcggc ccgaccgacc gcacccgcag
     2461 gtgaccgatg cgcataccga gcacgtacag cgtggcgaag tagagcgtcg cgccgccggc
     2521 gaccagcacc agcgtccatt ccatgcgctt catcgtgcgc atcgccatcc agtccgaggt
     2581 gtctccggcg acaaaccaca ggaacagtcc catcaccacg tttgccacga gcacctgaat
     2641 cagcatggcg cgccagccgg gcgcgtggtg caggacgtgg gcgcggcgca ggccccggta
     2701 gagctgtgcg gcgttgtacc aggcggagat gccgatgttg agcgcgagca gcgcgtgcag
     2761 gcccggtcgg ttgccgtaga gcgcgagcgg cagcacgacg aacacgttga gcgccatgtt
     2821 gacgacgagc gagcgtatgc cgatcttcat cggcgtgcgg gtgtcctggc gcgcgaagaa
     2881 tcccggggcc agtaccttca cgaggctgaa ccccatgagc cccgtcgcga acgccatgac
     2941 cgcgagcatg gacatgcgca cgtcctcggc attgaactcg ccatagtgga agatcgccac
     3001 ggtgagcggc ccggccagca cgatgagtgc gacggtcgcc ggcgccacca cgatgatcgt
     3061 gaggcgcagc gcccagtcca gcgtggcggc gaagcgctcg atcgattcgg ccgcgtggtg
     3121 ggccgcaagc ccggggagga tcacagtcgc gagtgccacg ctgaagacgc cgagcgggaa
     3181 ctccatcagg cggtcggcgt agtacagcca cgagatgctg ccggtcacca ggaacgacgc
     3241 gatcagcgta tcgagcagca gtgccacctg cgagaccgac gagccgaaga tcgccggcag
     3301 catcagctgg ccgatcttgc gcacgccggg gtggtgccat tgccagcgcg gacggcgcag
     3361 caggccgaga cgcaccatcg acggcagctg gaacgcgagc tgcacggcac ccgcgacgaa
     3421 cacgccgacc gcgagcacca ctcccggctt cgggaaccgc ggcgcgatcc acgcggcgaa
     3481 cgcgatcatc accaggttca tcagcgtcga catgagcgcc gggacgctga atcgctggta
     3541 gctgttcagc acgcccgacg ccagcgaagc gagcgagatg aacagcaggt acgggaacgt
     3601 ccagcgcagc atgtccaccg tgagctcgaa cttgcccgca tcggcgtgga aaccgggcgc
     3661 gaacaggagc acgaagacgg gcgccgccag gattcccacg atggtcacga ccgacatgaa
     3721 ccacgccatc gtgcccatcg cgccgtcgac gaggtcgcgc acctcgtcgt gggtgcgctg
     3781 gaccttgtac tcggagacga ccggcacgaa ggcctgcgag aaggccccct cggcgaacag
     3841 ccggcgcatg aagttcggga tcttgaacgc gaccaggaac gcgtccatca gcacgccggc
     3901 gccgaacatc cgcgaataga ccatgtcgcg gagcaggccc gtgaccctgg agagcagggt
     3961 cagcccgccg acgatgctgg tgctgcggaa gaagccgctc aagcgagggc cgtcgcgggc
     4021 gccgcgactg gtgcaggaat ggccggcatg ggggacattc tccattttcg cgacgagaaa
     4081 atggagaatg tccccatacc ctctgctttc gcctcgagaa aaaggagaat gtccccaccc
     4141 ttgaccggcg gcgaccgccc cgccagaata tgcggctctt ttaggctgat cgtgccggca
     4201 agccccggcc ccgcaggaga ccgtttccgt ggcgaacatc aagtccgcag aaaagcgcgc
     4261 caagcaggcc gtcaagcgcc gtgcccacaa catggccgcc cggtcgcggc tgcgcagctc
     4321 gatcaagaag gtgctggccg ccgtggcagc gggcgacaag gccggcgcgt ccgccgcgtt
     4381 caaggccgcg cagccggtga tcgacacgat ggtgaccaag ggcatcatcc acgcgaacaa
     4441 ggcttcccgc cacaagcgcg aactgaatgc caaggtgaag gcgctggccg cgaagtaacg
     4501 cgcctcgccc gacagatgcg aaaaagccgg ctccagggcc ggcttttcat ttggggcctg
     4561 accccaattc cccctactcc gccgacgccg accgcgcgtc ggccgcaatt tcgtcgagcc
     4621 tccgcatcac ggcctggcac agctcgcgcg tccccgcgcg tgttgccccg gagatgcgga
     4681 acaccgggcc cttccagcgc agcctgcgca cgatctcccg gcaacgcttg tcggccgcat
     4741 cggccgggag caggtccagc ttgttcaaca ccagccagcg ctcgcgcgcc gcgagttccg
     4801 ggctgaactt cttcagttcc gccgcgatcg cgcgcgcatc cttgaccggg tctgcgtccg
     4861 ggtcgggcgg cgcgatgtcc accaggtgca gcagcaggcg cgtgcgctgc aggtgcctga
     4921 ggaagcggat gccgaggccc gccccttccg ccgcgccctc aatcaggccg gggatgtcgg
     4981 ccatcacgaa gctcttgtgc tcgccgacgt acaccacgcc gaggttcggg tgcagcgtcg
     5041 tgaacgggta atccgcgatg cgcgggcgcg ccgccgacac cgcgctgatc aaggtcgact
     5101 tgcccgcgtt cggcagtccg agcagcccga cgtcggcgat cagcttgagt tccagccgca
     5161 gttcgcgctt ctctccgggg aaacccgggg tcgccttgcg gggcgtgcga ttggtgctgc
     5221 tcttgaagtg ctggttgccg agcccgccct tgccgccctt cgcgacgagg acccggtcgc
     5281 cgccgtgcgc gaggtcgccg agcacttcct cggtgtcgac gtcgtacacg accgtgccga
     5341 c
//
LOCUS       DAOMFD010000131         5325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6245_length_5325_cov_28.048956, whole genome shotgun sequence.
ACCESSION   DAOMFD010000131 DAOMFD010000000
VERSION     DAOMFD010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6245_length_5325_cov_28.048956"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(44..844)
                     /locus_tag="PL152_07920"
     CDS             complement(44..844)
                     /locus_tag="PL152_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49248.1"
                     /translation="MDRPIVELRDFIAASVWSARRTRTLMTILVVACVLAFGGFLNSL
                     SSSWMLQRIKAAGDPAGAYVIGKLGVATALDDPRYDAFYNALVKSYIDNAMSIDVPFF
                     GVAFDINDLGLLGGISFVTVLVLMRFSLRTEIVSLRLAFKAAARDAQGDREQLERFYD
                     LLASQQVFTLPHMRDASPGWMGRRPWLLRVGPKLTCFLPLAVYTLVAVNDFLSQGVAN
                     SISPLHTRLLLAYTAVIWVVIGVLSAWCFRRLVEIDRLWDDYWSRIQE"
     gene            complement(851..1507)
                     /locus_tag="PL152_07925"
     CDS             complement(851..1507)
                     /locus_tag="PL152_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49249.1"
                     /translation="MTNFDIIARAPLLAATASTLLLAAPGLVAQQETPIIQQDIEVVV
                     DRLGNAALSARFTLPAAQWQLWKQAYGENESLLKRDFQHQYSAVVLEDFKLERDDMNR
                     TATLRMKGEANAEYRGNGQWEVELEKGLRGTRVSDTEWHFTRTSAESGAVLQQNFILK
                     LPAGARGTAEGTSELGVPVLKYELKPQARSPLLPVLGGVLALLGLGAIAMGLIRKPAA
                     "
     gene            complement(1510..3249)
                     /locus_tag="PL152_07930"
     CDS             complement(1510..3249)
                     /locus_tag="PL152_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49250.1"
                     /translation="MKRPVLFVVGGVLLVASAACFLAFFLGRPDPGRIGMRIEVKPAV
                     MTAAYKVYANRTAGDGRYWLTKTILKNDGGRPLKNVSTSYQVPGLIGWTTPRTYPEVL
                     PGQTIVSLFYPHFPDSVASKTTNTTEVVELRVEYDGVTGRQQETRQVEFQMRGRNDMI
                     YTTLPASELVNPHDYYENTDLFAVFVTPDDPVIKYFVQELEQRVMGGTTVGAGADVQE
                     VGRFMKAVYSYWVASGIVYAGTEGLPEKVGETVTSIQHVRLPREVLTGKAGLCIELAA
                     LYSTIAEAAGLEPVIVTTANHAFPAIKVGGDLVAIEATAIGTPGMGEDSGPMSFGSAQ
                     RLGDAELSAWMQGGGDVVIDGHKVGELAIPIAAYDIADLHSKGIVPPELPDDAALKQK
                     IDDTFDQLLSRSGGRQASAGSTSRPQVAAEAAQARDSAPGNAYNDPGGRYSIVIPAGW
                     QAAVQPNPATPWVALQAMDPGTGIGLEVYVFEGVASVQAVMDHIQAGVASMGGVMQYT
                     PQGSANLGGRSFQLFTGGTAFPSAGRATDWDAYVRAAGNRTYVLSLGTTPGRLAAARP
                     VLRQVAGTFRVRG"
     gene            3551..4252
                     /locus_tag="PL152_07935"
     CDS             3551..4252
                     /locus_tag="PL152_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49251.1"
                     /translation="MRYVLVAMTLLFGSVTPAVAGVNVTIGVPVVNVGFDVPGYPQFT
                     IVPNYPVYYAPNVNTNLFFYDGLYWAYHHDNWYASSWYNGPWSMVNPVYVPTYILQVP
                     VRYYRVPPPYFHGWPKNKPPRWGQYWGPGWEHERHGWTTWRPTTVVVPAPLPVYQREY
                     PYAHYPRLEEQGEIRERHYDYRPSDPVARYQYEQHYSGPGGQSNGPPHGNNGMPPGQA
                     KKQGHGHDNPQGDRR"
     gene            4378..4569
                     /locus_tag="PL152_07940"
     CDS             4378..4569
                     /locus_tag="PL152_07940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49252.1"
                     /translation="MLRENNELDHDQPPTTDGLVVEETRDADTLRQFVRAWRRRVNHE
                     LAHSLLEDRPHDRHEAWFR"
     gene            4577..>5325
                     /locus_tag="PL152_07945"
     CDS             4577..>5325
                     /locus_tag="PL152_07945"
                     /inference="COORDINATES: protein motif:HMM:NF016777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR49253.1"
                     /translation="MIIDAWAQHPTARHLNQPMFESLRRWTRAEGRGLSADADWPVAV
                     TVAAMDEARVDRSLISAWVGPMGPLISNDEVAGFVRQAPQRLAGVGSVDIRRPREAVK
                     EIRRCVRELGFKAIRILPWLWELPPTHARFYPVYSECCELGVPFCTQVGHTGPLMPSE
                     VGRPIPYIDQVAIDFPELVIVCGHIGYPWTEEMIAVATKHVNVYIDTSAYTAKRYPQA
                     LVDYLKSHGGRKVLFGSNWPMIAPKKALEDL"
BASE COUNT          876 a         1799 c         1806 g          844 t
ORIGIN      
        1 ccaggcagtc ctttgatgcc cgccgcgcaa gagcccggac ggctcattcc tggatgcggg
       61 accagtagtc gtcccagagc cggtcgatct cgaccaggcg ccggaagcac cacgccgaca
      121 gcacgccgat gacgacccag atcacggccg tgtaggcgag cagcaggcgc gtgtgcagcg
      181 ggctgattga attggccacg ccctgcgaca ggaagtcgtt caccgcgacc agcgtgtaga
      241 ccgcgagcgg caggaagcag gtcaacttcg gcccgacccg cagcagccac ggccggcgac
      301 ccatccatcc gggcgacgcg tcgcgcatgt ggggcagggt gaagacctgc tgcgacgcca
      361 gcaggtcgta gaatcgctcg agctgctcgc gatcaccctg cgcgtcgcgg gcagcggcct
      421 tgaaagccag ccgcaggctc acgatctcgg ttcgcaggct gaaccgcatc agcaccagca
      481 cggtgacgaa gcttatgccg ccgagcaggc ccaggtcgtt gatgtcgaac gccactccga
      541 agaatggcac gtcgatgctc atggcgttgt cgatgtagga cttcaccagc gcgttgtaga
      601 aggcgtcgta gcgcggatcg tcgagggcgg tcgccacgcc gagcttgccg atgacgtagg
      661 cgccggccgg gtcgccggcc gccttgatgc gttgcagcat ccacgagctc gacagcgagt
      721 tcaggaaccc gccgaaggcg agcacgcacg cgaccaccag tatggtcatt agcgtgcgcg
      781 tgcgtcgcgc ggaccagacc gaggcagcga tgaagtcgcg cagctcgacg atcggacggt
      841 ccatgcgatc tcacgccgcc ggcttgcgga tcaagcccat ggcgatggcc ccgaggccga
      901 gcagcgccaa cacgccgccg agcaccggca gcagcgggga ccgcgcctgt ggcttcaact
      961 cgtacttcag caccggcacg cccagttcgc tggtgccttc cgcggtgccg cgtgcgcccg
     1021 ccggcagctt cagaatgaag ttctgctgca gcacggcgcc ggactccgcc gaggtccgcg
     1081 tgaagtgcca ctcggtgtcg ctgacccgcg tgccgcgcag gcccttctcc agttcaacct
     1141 cccactgacc attcccgcgg tattcggcgt tcgcctcgcc cttcatgcgc agcgtcgccg
     1201 tgcggttcat gtcgtcccgc tcgagcttga agtcctcgag cacgacggcc gaatactggt
     1261 gctggaagtc gcgcttcagc agcgactcgt tctccccgta ggcctgcttc cacagctgcc
     1321 actgggccgc gggcaaggtg aatcgcgcgg acagggcggc gttgccgagc cggtcgacca
     1381 cgacctcgat gtcctgctgg atgatcggcg tctcctgctg cgcaaccagt ccgggggcgg
     1441 ccagcagcag ggtggatgcg gtcgctgcca ggagcggcgc gcgggcgatg atgtcgaagt
     1501 tcgtcatggt cagcccctca cgcggaacgt cccggcgacc tggcgcagca ccggacgcgc
     1561 ggcggcgagc cggcccggcg tcgtgccgag gctcaagacg taggtgcgat tgccggcggc
     1621 gcgcacgtag gcgtcccagt ccgtggcgcg gcccgcggac gggaaggcgg tgccgcccgt
     1681 gaagagctgg aacgagcgcc caccgaggtt cgcgctgccc tggggcgtgt actgcatgac
     1741 cccacccatg gaggcaacgc ccgcctggat gtgatccatc accgcctgca cgctggcgac
     1801 gccttcgaac acgtagacct cgagcccgat gccggtgccg gggtccatcg cctgcagtgc
     1861 cacccagggc gttgcgggat tcggctgcac ggccgcctgc cagccggcgg gaatcactat
     1921 cgagtagcgg ccgcccggat cgttgtacgc gttgcccggt gcggaatcgc gcgcctgcgc
     1981 ggcctccgcg gcgacctgtg gccgcgacgt cgagccggcc gatgcctgtc gaccacccga
     2041 tcggctgagc agttggtcga acgtgtcgtc gatcttctgc ttgagggccg cgtcgtccgg
     2101 caactccggc ggcacgatgc ccttgctgtg caggtcggcg atgtcgtacg cggcgatcgg
     2161 aatcgcgagc tcgccgacct tgtggccgtc gatgacgacg tcgccgccgc cctgcatcca
     2221 cgccgaaagt tccgcgtcgc ccaggcgctg cgccgacccg aagctcatcg gtccgctgtc
     2281 ctcgcccatg ccgggcgtgc cgatggcggt cgcctcgatc gcgaccaggt cgccgccgac
     2341 cttgatggcc gggaacgcgt ggttcgccgt cgtgacgatg acgggctcga ggccggccgc
     2401 ctccgcgatc gtcgagtaga gcgccgccag ttcgatgcag aggccggcct tgccggtcag
     2461 cacctctctc ggcaggcgca cgtgctggat cgacgtgacg gtctcgccga ccttctccgg
     2521 caggccctcg gtgcccgcat acacgatccc gctcgcgacc caatacgagt agacggcctt
     2581 catgaagcgc ccgacttcct ggacgtcggc gcccgcaccg accgtggtgc cgcccatgac
     2641 gcgctgctcg agttcctgca cgaagtactt gatcaccggg tcgtcgggcg tgacgaagac
     2701 cgcgaacagg tcggtgttct cgtagtagtc gtgagggttg acgagttcgg aggccggcag
     2761 cgtcgtgtag atcatgtcgt tgcggccgcg catctggaac tcgacctggc gggtctcctg
     2821 ttgccggcct gtgaccccgt cgtactcgac acgcagttcc acgacctccg ttgtgttggt
     2881 cgtcttggag gcgaccgaat ccgggaaatg cgggtagaac agcgagacga tggtctgtcc
     2941 cggcaggacc tcggggtacg tgcgcggggt cgtccaaccg atgaggccgg gcacctggta
     3001 ggaggtgctg acgttcttca gtggccgtcc accgtcgttt ttcaggatgg tcttcgtgag
     3061 ccagtatcgg ccgtcacccg cggtgcggtt ggcgtagacc ttgtaggccg cagtcatgac
     3121 cgctggcttg acctcgatcc gcatgccgat gcgaccggga tccgggcggc ccaggaagaa
     3181 cgcgaggaaa cacgcggcgg atgccaccag gaggacgccg ccgaccacga acaagacggg
     3241 gcgcttcatc agggactcct tcctccggat gacactgcga ttctaggagc ctgctcgcag
     3301 gcgtttgagt cgcactagtg gaacggcgct gccggtcagt acctagcgcg ccggtcgatc
     3361 cgggcaggct gcgcgaccga atatttcgtg acgctcgtcg ccaagggcgg acggccccgg
     3421 ggcgtggacc gggaactccg ctaacctgcc cgtgtccgag attatgagca ggcagcccgc
     3481 cggaccgagg ctgggccctt gaggcgctgt gcggcgaccg ccagcgccgg gaactggagg
     3541 aggagacgcc gtgcggtacg tactcgttgc aatgacactg ttgttcggat cggtcacgcc
     3601 cgcggtggcc ggcgtcaacg tcacgatcgg tgtccccgtg gtcaacgtcg gcttcgacgt
     3661 gccgggttac ccgcagttca cgatcgtgcc gaactacccc gtctattacg caccgaacgt
     3721 gaacaccaac ctgttcttct acgacgggct gtactgggcg tatcaccacg acaactggta
     3781 cgcgagttcc tggtacaacg gcccctggtc gatggtgaac ccggtgtacg tgccgacgta
     3841 catcctgcag gtcccggtgc gctattaccg cgtgccgccg ccgtacttcc acgggtggcc
     3901 gaagaacaag ccgccgcgct ggggccagta ttggggccca ggctgggagc acgagcggca
     3961 cggctggacg acctggcgcc cgacgaccgt cgtcgtaccg gcaccgctac cggtgtacca
     4021 gcgcgagtac ccgtacgcgc actacccgcg cctcgaggag cagggcgaaa tccgcgagcg
     4081 gcactacgac tatcgtccga gcgacccggt tgcgcgctac cagtacgaac agcactactc
     4141 gggcccgggc gggcagtcca acgggccgcc gcacggcaac aacggcatgc cgccggggca
     4201 ggccaagaag caagggcacg gccacgacaa tccgcagggt gatcgccgct gatccaggcc
     4261 ggccgtggac gcccgagcgt cgcggcggcg aggtacgcag aaccccgtat tcccaaggct
     4321 gggccccgtg acagcatcgg cccataacaa caggccgcct cacaggcagg agggggcgtg
     4381 ctgcgggaaa acaacgagtt ggatcacgac cagccgccca cgaccgacgg gctggtggtc
     4441 gaagaaacac gcgacgcgga cacgcttcgg cagtttgtcc gcgcgtggcg ccggcgcgtg
     4501 aatcacgaac tggcgcactc cctgctggaa gaccggccgc acgatcgtca cgaggcctgg
     4561 ttccgctgag ttgcgcatga tcatcgacgc gtgggcccag catccgacgg cccgtcacct
     4621 gaaccagccg atgttcgagt cgctgcggcg ctggacgcgc gccgagggtc gcggcctgtc
     4681 ggcggacgcc gattggcccg tggcggtcac cgtcgctgcg atggacgagg cccgggtcga
     4741 ccgcagcctc atctcggcgt gggtcgggcc gatgggtcca ctgatctcca acgacgaagt
     4801 cgccggcttc gtgcgccagg cgccccagcg gctcgccggc gtgggctcgg tggacatccg
     4861 ccgaccgcgt gaagcggtca aggagattcg gcgctgcgtc cgcgaactcg gcttcaaggc
     4921 catccggatc ctgccgtggt tgtgggaatt accgccgacc cacgcgcgct tctacccggt
     4981 gtacagcgag tgctgcgagc tgggcgtccc gttctgcacg caggttggtc acacggggcc
     5041 gctcatgccg tccgaggtcg gtcggccgat cccgtacatc gaccaggtgg cgatcgactt
     5101 cccggaactc gtcatcgtct gcgggcacat cggctacccg tggacggaag aaatgattgc
     5161 ggtggcgacc aagcatgtga acgtctacat cgacacgtcc gcctacacgg ccaagcgtta
     5221 cccgcaggcg ctggtcgact acctcaagtc gcacggcggg cggaaggtcc tgttcgggtc
     5281 caactggccg atgatcgcgc cgaaaaaggc gctcgaggac ctcga
//
LOCUS       DAOMFD010000132         5309 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6283_length_5309_cov_31.035782, whole genome shotgun sequence.
ACCESSION   DAOMFD010000132 DAOMFD010000000
VERSION     DAOMFD010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5309)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5309
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6283_length_5309_cov_31.035782"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1648
                     /locus_tag="PL152_07950"
     CDS             <1..1648
                     /locus_tag="PL152_07950"
                     /inference="COORDINATES: protein motif:HMM:TIGR03504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HQR49254.1"
                     /translation="PAGAPAQPPAGAAQAPEGAAPGVAPEAAQPAPEAAAPAQAQPAP
                     TPAPEVKKPTPKPKPAARPPAEAGPSLFERLAGYWWVAAGLLAAALAFVLYRRSKGES
                     AGKEVDLQEALARTRTDLRARPAPRDTAIVVEERRPVGPAAVTKVSAAEAPRAPEPTR
                     KPVSIEDTISGEEPVSIEAGDPLAEADFHMAYGLYDQAADLVQLAIKRAPQRRELKLK
                     LLEIFFVWGNRDRFLELAREMQASRADAPAGEWDKILIMGKQIAPDEPIFAGPAPSLK
                     TSLDMELHSARGPLDMDLGADQGAAPDVDLSEGAARSPDESGLDFVFNEPAVGTDSTS
                     LVPTVEMPQVPTVEIAQVPTVEMPQVATVEMPHIKAQRAASLEPTQELPVEELGLDVG
                     NLRSLDDTSGGEDLLALSPRSVVEDTVESPRIARKAGRLDDEEDLLDATGMLKEQTGI
                     LKALDSTAELSELQYGEATGELPTIETARIERGAVGEVDFDLGSEPSTMSEVGTKLDL
                     ARAYVDMGDPEGARSILDEVLKEGSSDQKKEAERLIASLP"
     gene            1656..2432
                     /gene="truA"
                     /locus_tag="PL152_07955"
     CDS             1656..2432
                     /gene="truA"
                     /locus_tag="PL152_07955"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HQR49255.1"
                     /translation="MARIAIGVEYDGSAYSGWQAQLHAPSVQGELERALSRVADHRIE
                     VTAAGRTDAGVHALMQVAHFDTVAARPEQAWVLGGSAESAADVSVLWAREVPDDFHAR
                     FSALSRGYVYRIVNRKVRPALDRARACWVRRPLDADAMQAAAQCLLGEHDFSAFRAAE
                     CQSTTPVRRVTAVAVRRDADRVEMRIEANAFLHHMVRNVAGTLIRVGVGDEPTEWVDD
                     VLRSRDRTRAGATAPPQGLYFAGVDYGTRFRLPSWLLALR"
     gene            2461..3339
                     /gene="accD"
                     /locus_tag="PL152_07960"
     CDS             2461..3339
                     /gene="accD"
                     /locus_tag="PL152_07960"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HQR49256.1"
                     /translation="MTWFEKIMPSRIKTERRTRSVPEGLWIKCPACDAVLYRAELERN
                     LHVCPKCAHHMRITARERLLRFLDEGSAREIAMEVEPEDPLRFRDSKKYRDRLVAAQK
                     HTGEKDALIVMVGAVRGLELVAAAFEFRFLGGSMGSVVGERFVRGAEHCLEHRLPLVC
                     FSASGGARMQEGLLSLLQMSKTSAALARMADAGLPFISVLTDPTTGGVSASLAMLGDL
                     NIAEPRALIGFAGPRVIEQTVRETLPEGFQRSEFLLEHGAIDLIVDRRDLRDRIGALL
                     AMLTGRPPLEAPAEPA"
     gene            3341..4657
                     /gene="folC"
                     /locus_tag="PL152_07965"
     CDS             3341..4657
                     /gene="folC"
                     /locus_tag="PL152_07965"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF008101.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HQR49257.1"
                     /translation="MPTGPRFADLGRWLEWQQMLHPGAIDLGLARVGRVLARTGWQRP
                     RCPVITVGGTNGKGSCVALLAAMLKAAGCRVGTFTSPHLVEYSERIRIDGTPVSEASL
                     VAAFERTADGLAPDSLTYFEFNTLAALLVFETAGLDAIVLEVGMGGRLDAVNLVDADV
                     AVIVSVGLDHMEWLGPDVESIGREKAGIMRRGRPAIVGMARPPQSVLQSAAEVGADLR
                     LRSRDFDGEERDDGTWTYRDRDGQLDALPAPALPGAVQVGNAAVALAALRALDGRMHL
                     ERSAIEAGLRDVRLPGRFQRIEDRRGFEWVLDVAHNPDSARTLAGNLARYPVAGRTIA
                     VCGMLSDKDVESVVTTLRAHVQTWIAADTAGPRGLSAAALAERAAAGGSSMVPGGGVA
                     DAMRRAASEASPGDRIVVFGSFHTVGPALVHLRETLPPGEAARLWL"
     gene            4666..>5309
                     /locus_tag="PL152_07970"
     CDS             4666..>5309
                     /locus_tag="PL152_07970"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQR49258.1"
                     /translation="MDDALKARLIGAAVLVALAVILIPELLSGRKPESSDAAPSAAAR
                     GTRTVTIDLTGASPGAAAAPVAAQPMASSAQGASAVTTTAATPPVSADREPAARPLAS
                     PTPQATSPQPRPQPGVSPAAPAGTTAPQSPPTTKTVAAPAVAIPARGGWAVQVGAFGA
                     TATADKLARELQSAGFKAYVSPVARSGKTLYRVRVGPEAERSRAEGLAASLKGRG"
BASE COUNT          777 a         1836 c         1949 g          747 t
ORIGIN      
        1 tcccgccggc gcgcctgccc agccccccgc gggcgcggct caggctccgg aaggcgcagc
       61 gcctggagtt gctcccgaag ccgcgcagcc tgctcccgag gccgcggcac cggcacaagc
      121 ccagccggcg ccgacgccgg cgcccgaagt caagaagccg acgccgaagc cgaagccagc
      181 cgctcgaccg ccagccgaag caggtccatc gctgttcgag cggttggcgg ggtactggtg
      241 ggtcgccgcc ggactgctcg ccgccgcact ggcgttcgtg ctctacaggc gcagcaaggg
      301 cgagtcggcc ggcaaggaag tcgacctgca ggaggcgctc gcacggactc gcacggacct
      361 gcgtgcgcgg cctgcgccgc gtgacacggc gatcgtcgtc gaggagcgcc ggccggtcgg
      421 gccggcggcg gtgacgaagg tctcggccgc cgaagcgccg cgcgcgccgg aaccgacgcg
      481 caagccggtc tcgatcgaag acacgatctc cggcgaggag ccggtcagca tcgaggcggg
      541 agacccgctc gccgaagccg acttccacat ggcctatggc ctgtacgacc aggcggccga
      601 cctcgtccag ctggcgatca agcgtgcccc gcagcgccgc gagctgaagc tcaagctgct
      661 cgagatcttc ttcgtctggg gcaatcgcga tcgcttcctc gagcttgcca gggagatgca
      721 ggcgtcgcgc gcggacgcgc cggccggcga gtgggacaag atcctgatca tgggcaagca
      781 gatcgcgccg gacgagccca tatttgccgg gcccgcaccg tcgctgaaga ccagcctcga
      841 catggagctg cacagcgccc ggggaccgct cgacatggac ctcggtgccg accagggcgc
      901 cgcccccgac gtcgacctgt ccgaaggggc tgcgcgctcg ccggacgaga gcgggctcga
      961 cttcgtgttc aacgagccgg ccgtcgggac ggattcgacc tcgctcgtgc ccacggtcga
     1021 gatgccgcag gtacccaccg tcgagattgc gcaggttcct accgtcgaga tgccgcaggt
     1081 cgccaccgtc gagatgccgc acatcaaggc gcagcgggcc gcgtccttgg agccgaccca
     1141 ggaactcccg gtggaggaac tcgggctcga cgtgggcaat ctcaggtccc tggacgacac
     1201 gtcgggcggg gaagacctgc tcgcactgtc cccgaggtcg gtcgtcgagg acaccgtcga
     1261 gagcccgcgc atcgccagga aggccgggcg gctcgacgac gaggaagatc tcctcgacgc
     1321 caccggcatg ctcaaggaac agaccggcat cctgaaggcg ctggactcga ccgccgagct
     1381 gtccgagctg cagtacgggg aagcgacggg cgaattgccg acgatcgaga ccgcgaggat
     1441 cgagcgcggt gccgtgggcg aggtcgactt cgacctgggc tcggagcctt cgacgatgag
     1501 cgaggtgggc accaagctcg acctcgcccg cgcgtacgtg gacatgggcg atccggaagg
     1561 cgcacgcagc atcctcgacg aggtgctgaa ggaaggcagc tcggaccaga agaaggaagc
     1621 cgagcggctg atcgccagcc tgccctgacg ggcggatggc gcggatcgcc atcggcgtcg
     1681 agtacgacgg atcggcctac tcgggctggc aggcgcagct gcacgcgccg agcgtgcagg
     1741 gcgaactcga gcgcgcgctc tcccgagtgg cggaccaccg tattgaggtg actgcggctg
     1801 ggcgcaccga cgccggcgtg cacgcgctca tgcaggtggc gcacttcgac acggtcgcgg
     1861 cgcgacccga gcaggcctgg gtgctgggtg gttcggcgga atcggccgcg gacgtgagcg
     1921 tgctgtgggc ccgcgaggtc cccgacgact ttcacgcgcg cttcagcgcc ctgtcgcgcg
     1981 gctatgtgta tcgcatcgtc aaccgcaagg tgcggcctgc gctcgaccgt gcgcgcgcct
     2041 gctgggtgcg gcgcccgctc gatgccgacg cgatgcaggc ggctgcgcag tgcctgctcg
     2101 gcgagcacga cttctccgca ttccgggccg cggaatgcca gtccacgacg cccgtgcggc
     2161 gggtcactgc cgttgcggta cgacgcgatg cggaccgcgt cgagatgcgc atcgaggcca
     2221 atgccttcct gcaccacatg gtgcgcaacg tggcgggcac cctgatccgt gtcggcgtgg
     2281 gtgacgagcc gacggagtgg gtcgacgacg tgctgcgctc gcgtgatcgc acgcgggccg
     2341 gggccaccgc gccgccgcag ggcctgtact ttgccggcgt cgactacggg actcgctttc
     2401 ggctgccgtc gtggctgctg gccttgcggt agactgcccg cggccgccga ggaggagccc
     2461 gtgacctggt tcgagaagat catgccctcg cgcatcaaga cggagcgccg cacgcgttcc
     2521 gtgcccgagg gactgtggat caagtgcccg gcctgcgacg cagtgctgta tcgggcagag
     2581 ctcgaacgca acctccacgt gtgcccgaag tgcgcgcacc acatgcgcat caccgcgcgc
     2641 gagcggctgc tgcgcttcct cgacgaaggc tcggcccggg aaatcgccat ggaagtcgag
     2701 cccgaggacc cgctgcgttt ccgcgacagc aagaagtacc gtgatcgcct ggtcgcggcc
     2761 cagaagcaca cgggcgagaa ggacgcgctc atcgtcatgg tgggagcggt gcggggcctc
     2821 gagctggtcg ccgcggcatt cgagttccgt ttcctcggcg gctccatggg ctcggtcgtc
     2881 ggcgagcgtt tcgtgcgcgg ggcggagcat tgcctggagc atcggctgcc actcgtgtgc
     2941 ttttccgcga gcggcggggc gcgcatgcag gaggggctgc tgtcactgct gcagatgagc
     3001 aagacgagtg cggccctggc gcgcatggcc gacgcggggc tgccgttcat atccgtgctc
     3061 accgatccca cgaccggcgg cgtctcggcg agcctcgcga tgctcggcga cctcaacatc
     3121 gccgagccgc gggcgctgat cggcttcgca gggccgcgcg tgatcgagca gacggtgcgc
     3181 gagacgctgc ccgaaggctt ccagcgcagc gaattcctgc tcgagcacgg cgcgatcgac
     3241 ctgatcgtgg accgccgcga cctgcgcgac cgcatcggcg cgctgcttgc gatgctgacg
     3301 ggccggccgc cgctggaggc tccggccgag ccggcctgag atgccgaccg gcccgcgatt
     3361 cgccgacctg ggccggtggc tcgagtggca gcagatgctg cacccaggag cgatcgatct
     3421 cggcctcgca agggtcggcc gcgtgctcgc gcgaaccggc tggcagcgcc cgcgttgtcc
     3481 cgtcatcacc gtcggcggca ccaatggcaa gggttcctgc gtcgcgctgc tcgcagcgat
     3541 gctcaaggcc gccgggtgcc gtgtcggcac gttcacgtcg ccgcacctcg tcgagtacag
     3601 cgagcgcatc cgcatcgacg gcactcccgt gtcggaggcg tcgctcgtgg ccgcattcga
     3661 gcgcaccgcc gacgggctcg cgcccgactc actcacctat ttcgagttca atacgctcgc
     3721 ggcgctgctg gtgttcgaga cggcgggtct cgatgcgatc gtgctggagg tcggcatggg
     3781 tggccggctc gacgcagtca acctcgtcga cgccgacgtg gcggtcatcg tctcggtggg
     3841 gctcgatcac atggagtggc tgggaccgga cgtcgagagc atcggtcgcg agaaggcggg
     3901 gatcatgcgc agggggcggc ccgccatcgt cgggatggcg cgcccaccgc aatcggtgct
     3961 gcagtcggcc gccgaggtgg gcgcggacct caggctgcgc agccgggact tcgacggtga
     4021 ggagcgggac gacgggacct ggacctaccg cgaccgcgat gggcaactcg acgccctgcc
     4081 ggcacccgcg cttccgggag ccgtgcaggt gggcaacgcc gccgtggcgc tggcagcgct
     4141 gcgcgcgctc gatgggcgga tgcacctcga gcgttcggcg atcgaggcgg ggttgcgcga
     4201 cgtgcggttg ccgggacgat tccagcgcat cgaggatcga cggggattcg agtgggtgct
     4261 ggacgtggcg cacaatcctg attcggcgcg gacgctggcc gggaacctcg cgcgctaccc
     4321 ggtcgcgggt cgcacgatcg ccgtatgcgg catgctgtcg gacaaggacg ttgagtcggt
     4381 cgtgaccacc ctgcgcgcgc acgtgcagac ctggatcgcc gcagacaccg caggccctcg
     4441 cgggttgtcc gccgccgcgc ttgcagagcg agcggccgcc ggcgggtcga gcatggtgcc
     4501 gggtggtggc gttgccgacg caatgcgccg ggctgcatcc gaggcgagcc ccggcgaccg
     4561 gatcgtcgtg ttcggatcct ttcacacggt cggcccggcg ctggtgcacc tgcgcgagac
     4621 attgccgccc ggcgaggccg cgcggctctg gctataattt tgcgcatgga cgacgcgctc
     4681 aaggcgagat tgatcggtgc agcggtgctc gtcgcgctcg cggtgatcct gatccccgag
     4741 ttgctgtcgg gccgcaagcc tgagtcatcc gacgccgcgc cgtcggcggc agcgcggggc
     4801 acgcgcaccg tcaccatcga cctgaccggt gcctcgccgg gagcggccgc cgcgcccgtg
     4861 gcggcgcagc cgatggcatc gtcggcacag ggcgcgagtg cggtcacgac gaccgctgcg
     4921 acgcctcccg tttcggcgga ccgcgagccc gcggcccggc cgctcgcctc acccacgccg
     4981 caagccacat cgccccagcc gcggccgcag cctggcgtgt cgccggctgc gcccgccggc
     5041 acgaccgccc cgcagtctcc accgacgacg aagaccgtcg cggcaccggc cgtggcgatc
     5101 ccggcgcgcg gaggatgggc ggtgcaggtc ggcgcgttcg gcgccaccgc gaccgccgac
     5161 aagctcgccc gcgagctgca gtctgcgggc ttcaaggctt atgtctcgcc ggtcgcgcgc
     5221 tcgggcaaga ccctgtaccg cgtccgggtc ggtcccgagg ccgagcgcag ccgggcggag
     5281 ggcctggcgg catcgctcaa gggccgcgg
//
LOCUS       DAOMFD010000133         5305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6291_length_5305_cov_35.763810, whole genome shotgun sequence.
ACCESSION   DAOMFD010000133 DAOMFD010000000
VERSION     DAOMFD010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6291_length_5305_cov_35.763810"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="tldD"
                     /locus_tag="PL152_07975"
     CDS             <1..1104
                     /gene="tldD"
                     /locus_tag="PL152_07975"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HQR49259.1"
                     /translation="WHARGGRGLYLPMDPVESLDDGDKVKLLEAVDREARALDSRIVQ
                     VMASLAASHDTILVLASDGTLAADVRPLVRLNVSVIIEQGGRREQGYAGGGGRFAYTE
                     FLAGERWRELVREAVRAAQVNLEAVPAPAGTMTVVLGPGWPGVLLHEAVGHGLEGDFN
                     RKGTSAFSGRIGQQVAARGVTVVDDGTLATRRGSLNVDDEGTPTGCTTLIEDGVLRGY
                     LQDKLNARLMGVAPTGNGRRESFAHLTLPRMTNTYMMPGPHAPEEIIGSVAKGIYCRH
                     FGGGQVDITSGKFVFSASEAYLIENGRLGAPVKGATLIGNGPDVMTRITMVGNDLKLD
                     DGVGTCGKEGQDVPVGVGQPTLRVDGLTVGGTA"
     gene            1117..1341
                     /locus_tag="PL152_07980"
     CDS             1117..1341
                     /locus_tag="PL152_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49260.1"
                     /translation="MGLWLPRRLYESLPYVYMAAGLGALVLAFVRQRDPHGVLMLFGG
                     LALTVGLVLWMRRREYRDNQAEYDPHSLDD"
     gene            complement(1348..2703)
                     /locus_tag="PL152_07985"
     CDS             complement(1348..2703)
                     /locus_tag="PL152_07985"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR49261.1"
                     /translation="MYLEHFQLKELPFRLSPDPAFLYLSQIHSRAKAYMESTIWFTDG
                     FVVITGEIGSGKTTLIETFLREIDQDVVVAQINQTQVTPIEFLQTLLAQFGFSPFRMR
                     KAELLATLNDFLIEQHANGRRVLLIVDEAQNLSNKVLEEVRLLSGVETTKEKVLRIIL
                     AGQPELNDKLDSVDLVQFRQRVRLRFHLTALSEEDTVAYVRHRLAVAGAGEREIFEPE
                     TFPIIYRYAGGIPRLVNTLCDTALLAAFAQDRTSVSAADVMSAVSELQWVEYAARTAS
                     HRQFHVETTASHEQPPVGRLVVKLKGAQVGELNLLPGRRFIGRTSDNDLQIDSRYVSR
                     HHAQVITTTDGSWIEDLNSTNGIFVRGRRVRRHRFQEGDTVDIGVHSICYEHVQPLVT
                     ATTVLPKDALTDESDEEDDDLDSDLEPESEPEVGAEAETEAEVEVEAGAEAGAADERD
                     S"
     gene            complement(2786..3877)
                     /locus_tag="PL152_07990"
     CDS             complement(2786..3877)
                     /locus_tag="PL152_07990"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HQR49262.1"
                     /translation="MRIGVIGAGQLGQMLALAGHPLALEFLFLDPSPDSPGARVGRVL
                     AGAFDEPASLRTLAEGSDLVTYEFENVPVAALEAVSKTRTCLPPVEALRVSQDRLLEK
                     ELFTRLGIPTPAFRAVDSLADLRAAVADLGLPCVLKTRRLGYDGKGQQYLRRAQDVET
                     AWAALGGAPLILEGFVKFDHEVSIVGARSTRGETRCYPLADNEHRDGILRVTRAPYRN
                     ARLQRAAERHLRRVLDHFGYAGVLTIEFFVQGGRLMANEMAPRVHNSGHWTIEGAVTS
                     QFENHLRAILGLPLGDTAAVGHAAMVNYIGTMPDRDRVLAIPGAHHHDYGKQPRAGRK
                     LGHATLVAPTRAVRERLLGRLLRLVPRQG"
     gene            complement(3874..4353)
                     /gene="purE"
                     /locus_tag="PL152_07995"
     CDS             complement(3874..4353)
                     /gene="purE"
                     /locus_tag="PL152_07995"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003021871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HQR49263.1"
                     /translation="MGSTSDWETLQSAAQVLDRLGVPYETRVVSAHRTPDLLFEYAAS
                     ARGRGLEVLIAGAGGAAHLPGMTASKTSLPVLGVPVQSKTLNGLDSLLSIVQMPAGVP
                     VGTLSIGTAGAANAALLAASILSLSHAAVRESLDAFRAEQTAKVLAQPDPRVPPAGA"
     gene            complement(4455..4964)
                     /gene="yjgA"
                     /locus_tag="PL152_08000"
     CDS             complement(4455..4964)
                     /gene="yjgA"
                     /locus_tag="PL152_08000"
                     /inference="COORDINATES: protein motif:HMM:NF016626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis factor YjgA"
                     /protein_id="HQR49264.1"
                     /translation="MGDTDDDEERPSRSERKRQSRDLQDLGWDLVGLPEAELAAMPLP
                     DDVRDAVETARRITSNGALARQRLYIGKLLRRIDVEPIQEALERRGELDRQRIRREKA
                     LEEWRDRLLADEAAAWTELATRIAPDVLQQMRVLVRQARAEQSAARPPAAARQLFRRL
                     RAALQAPDH"
     gene            5049..>5305
                     /locus_tag="PL152_08005"
     CDS             5049..>5305
                     /locus_tag="PL152_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase modulator"
                     /protein_id="HQR49265.1"
                     /translation="MSSERNLAPAVEGADLLALVEIALEEARALGATQAEAAISMDVG
                     LSVSVRLGEVETIEYQRDRGMGVTVYFGTRKGSASTADLSTA"
BASE COUNT          749 a         1826 c         1928 g          802 t
ORIGIN      
        1 tggcacgcgc gcggcgggcg cggcctgtac ctgccgatgg atccggtcga gtcgctggat
       61 gacggcgaca aggtcaagct gctcgaagcg gtcgaccgcg aggcccgtgc gctcgattca
      121 cgcatcgtgc aggtgatggc gagcctcgcc gcctcgcacg acacgatcct cgtgctcgcg
      181 agcgacggga cgctggcggc cgacgtgcgg ccgctggtgc ggctcaacgt gtcggtgatc
      241 atcgagcagg gcgggcgccg ggaacagggc tacgcgggtg gcggcggccg gttcgcgtac
      301 acggaatttc tcgccggcga gcggtggcgc gagctcgtgc gcgaggccgt gcgggccgcg
      361 caggtgaatc tcgaggcggt gccggccccg gcgggaacga tgacggtcgt gctcggtccc
      421 ggctggcccg gcgtgctgct gcacgaagcc gtgggccacg ggctcgaggg cgacttcaac
      481 cgcaagggta cctcggcgtt cagcggtcgc atcggacagc aggtcgcggc gcgcggcgtc
      541 acggtcgtcg acgacgggac gctggccacg cgccgcggct cgctcaacgt ggacgacgaa
      601 ggcacgccga cgggttgcac cacgctcatc gaggacggcg tgctgcgcgg ttacctgcag
      661 gacaagctga acgcgcgcct catgggcgtt gcgcccacgg ggaacggccg ccgcgagtcg
      721 ttcgcgcacc tcacgctgcc gcgcatgacc aacacctaca tgatgccggg cccgcacgcg
      781 ccggaggaga tcatcggctc ggtcgcgaag ggcatctact gccggcactt cggcggcggg
      841 caggtggaca tcacctccgg caagttcgtc ttctccgcga gcgaggcgta cctcatcgag
      901 aacggccggc tcggcgcgcc ggtcaagggt gcgacgctca tcggcaacgg gccggacgtg
      961 atgacgcgca tcacgatggt cggcaacgac ctcaagctcg acgacggcgt gggcacctgc
     1021 ggcaaggaag ggcaggacgt gccggtcggc gtcgggcagc cgacgctgcg cgtggacggc
     1081 ctcaccgtcg gcggcacggc ctgatcgagg cgtgacatgg gactgtggct gccccgcagg
     1141 ctctacgaat cgctgcccta cgtgtacatg gcggcggggc tcggcgcgct cgtgctcgcc
     1201 ttcgtgcggc agcgcgatcc gcacggcgtg ctcatgctgt tcggggggct ggcgctgacc
     1261 gtcgggctcg tgctgtggat gcgacgtcgc gagtaccgcg acaaccaggc cgagtacgac
     1321 ccacattccc tcgacgactg aggtccatca gctgtcgcgt tcgtccgcgg cgcctgcttc
     1381 cgccccggcc tcgacctcga cctcagcctc cgtctcggct tcggccccga cttcaggttc
     1441 ggactcaggc tcgaggtcgg agtccaaatc gtcgtcttcc tcgtcggact cgtcggtcag
     1501 cgcgtccttt ggcagcaccg tcgtcgccgt gaccagcggc tgcacgtgct cgtagcagat
     1561 cgaatgcacg ccgatgtcca ccgtgtcgcc ttcctggaac cggtgacggc gcacgcgacg
     1621 accgcgcacg aagatgccgt tcgtgctgtt caagtcctcg atccagctgc cgtcggtcgt
     1681 ggtgatgacc tgcgcgtgat gccggctcac gtagcggctg tcgatctgca ggtcgttgtc
     1741 ggaggtgcgg ccgatgaacc ggcgccccgg caggaggttc agttcgccga cctgcgcacc
     1801 cttgagcttc accaccagcc ggccgaccgg cggctgctcg tgcgacgccg tcgtctcgac
     1861 gtggaactgg cggtgcgacg cggtgcgcgc cgcatactcg acccactgca gctcgctcac
     1921 cgccgacatg acgtccgcgg cggagacgct cgtgcggtcc tgcgcgaagg cggccagcag
     1981 tgcggtgtcg cacagcgtgt tcacgagccg cgggatgccg cccgcgtagc ggtagatgat
     2041 cggaaacgtc tccggctcga agatctcgcg ctcgccggcc ccggcgaccg ccaagcggtg
     2101 gcggacgtag gcgacggtgt cttcctcgct gagtgcggtc aggtggaagc gcaggcgcac
     2161 gcgctggcgg aactgcacga ggtccacgga gtccagcttg tcgttcagct ccggctggcc
     2221 cgcgaggatg atgcgcagca ccttctcctt ggtcgtctcc acgccggaga gcaggcgcac
     2281 ttcctcgagc accttgttgc tgaggttctg cgcctcgtcc acgatcagca gcacccggcg
     2341 gccgttggcg tgctgctcga tcaggaagtc gttcagggtc gcgagcagct ccgccttgcg
     2401 catgcggaac gggctgaagc cgaactgcgc gagcagcgtc tgcaggaact cgatcggcgt
     2461 cacctgcgtc tggttgatct gcgcgaccac gacgtcctgg tcgatctccc gcaggaaggt
     2521 ctcgatcagg gtggtcttgc cggagccgat ctcgccggtg atcacgacga agccgtccgt
     2581 gaaccagatc gtcgactcca tgtacgcctt ggcgcgggag tggatctggc tcaggtacag
     2641 gaaggccggg tccgggctca gccggaacgg caattccttg agctggaagt gttccaggta
     2701 catgggggag ggctctggac ggcactataa aggaacggac cccggccgca ataaagtggt
     2761 cggtcacagt cctggtggcc ggcggtcagc cctgccttgg aacgaggcgc agcagacgcc
     2821 cgaggaggcg ctcccgcacc gcccgcgtcg gtgcgacgag ggtggcgtgt ccgagcttgc
     2881 ggcccgcccg gggctgcttg ccgtagtcgt ggtggtgggc gcccgggatc gccagcaccc
     2941 ggtcccggtc gggcatggtg ccgatgtagt tgaccatggc cgcgtggccc acggccgcgg
     3001 tgtcgccgag cggcaggccg aggatcgcgc gcaggtgatt ctcgaactgc gaggtgaccg
     3061 cgccctcgat ggtccagtgc ccggagttgt gcacgcgcgg cgccatctcg ttggccatca
     3121 gccgtccgcc ctgcacgaag aactcgatcg tgagcacgcc cgcgtacccg aaatggtcga
     3181 gcacgcggcg caggtgccgc tccgcggcgc gctgcagtcg cgcgttgcga tacggcgcgc
     3241 gcgtcacgcg caggatgccg tcgcgatgct cgttgtcggc gagcgggtag cagcgcgtct
     3301 cgccgcgagt gctgcgcgcg ccgacgatcg acacctcgtg gtcgaatttc acgaagccct
     3361 cgaggatcag cggcgcgccg ccgagcgcgg cccaggccgt ctccacgtcc tgcgcgcgac
     3421 gcaggtactg ctgccccttg ccgtcgtagc cgagccgtcg cgtcttgagc acgcagggca
     3481 ggccgaggtc ggcgaccgcg gcgcgcaggt ccgcgaggct gtccacggcg cggaacgccg
     3541 gcgtcgggat gccgaggcgc gtgaagagtt ccttctcgag cagccggtcc tgcgagacgc
     3601 gcagcgcctc gaccggaggc aggcaggtgc gcgtcttcga caccgcctcg agtgcggcga
     3661 ccggcacgtt ctcgaactcg taggtgacga ggtccgagcc ctcggccagg gtgcgcaggc
     3721 tggcgggctc gtcgaatgcg ccggcgagga cccggcccac gcgggcgccc ggggagtccg
     3781 ggctgggatc gaggaacagg aactcgaggg ccagcggatg gccggccagc gccagcatct
     3841 gtcccagctg gccggcaccg atgaccccga tcctcatgcg ccggcgggcg gtacgcgcgg
     3901 gtcgggctgc gcgaggacct tggccgtctg ctcggcgcgg aaggcgtcca gcgactcgcg
     3961 gaccgcggcg tgcgacaggc tcaggatgga cgccgcaagc agcgccgcat tggccgcgcc
     4021 ggccgtgccg atggacagcg tgccgacggg cacgccggcg ggcatctgca cgatcgacag
     4081 cagcgagtcg aggccattca aggtcttcga ctgcacgggc accccgagca cgggcagcga
     4141 ggtcttcgag gccgtcatgc ccggcaggtg cgcggctccg ccggccccgg cgatcagcac
     4201 ctcgaggccg cggccgcggg cggaggccgc gtactcgaac aacaggtccg gcgtgcggtg
     4261 cgccgatacg acccgcgtct cgtacgggac gcccaggcgg tccaggacct gtgccgcgga
     4321 ctggagggtc tcccagtccg acgtcgaccc catgatgatg ccaaccagcg gtttcaccgc
     4381 agcattgtag catcggcgtc ccgcggcctc gcagcgccgg ggaccggccg cgcccacggc
     4441 gtgggcgccg gccgctagtg gtcgggagcc tgcagggctg cgcgcaggcg cctgaacagc
     4501 tgtcgcgcgg cggccggcgg tcgtgcggcc gactgctcgg cacgcgcctg ccgcacgagc
     4561 acgcgcatct gttgcagcac gtcgggcgcg atccgcgtgg ccagctcagt ccacgccgcg
     4621 gcctcgtcgg ccaacaggcg gtcgcgccac tcctccaggg ctttttcgcg ccggatgcgc
     4681 tgtcggtcga gctcgccgcg gcgttcgagc gcttcctgga tcggctcgac gtcgatgcgg
     4741 cgcagcagct tgccgatgta cagccgctgc cgggccagcg cgccgttgct ggtgatgcgt
     4801 cgcgcggtct cgaccgcgtc gcgcacgtcg tccggcagcg gcatcgcggc gagttccgct
     4861 tccggcaggc cgaccaggtc ccatccgagg tcctgcaggt cacggctctg gcgcttgcgc
     4921 tcgctgcggc tcggtcgctc ctcgtcgtcg tccgtatcgc ccatcgcgcc tccgcagggg
     4981 atgctagcac gcgcggccgc cggcccggcc gctgctagaa tgccgcctcc ccggtatcag
     5041 gctcggtcat gtccagcgaa cgaaacctag cgcccgccgt cgaaggcgca gacctgctcg
     5101 cgctcgtcga gatcgcgctc gaggaggcgc gtgcgctcgg cgccacccag gccgaggcgg
     5161 cgatcagcat ggacgtcggc ctgtcggtgt ccgtccggct cggcgaggtc gagacgatcg
     5221 aataccagcg cgaccgcggc atgggggtca cggtgtactt cggcacccgc aagggatcgg
     5281 cgagcaccgc cgacctgagc accgc
//
LOCUS       DAOMFD010000134         5235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6431_length_5235_cov_27.406371, whole genome shotgun sequence.
ACCESSION   DAOMFD010000134 DAOMFD010000000
VERSION     DAOMFD010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6431_length_5235_cov_27.406371"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..817)
                     /locus_tag="PL152_08010"
     CDS             complement(<1..817)
                     /locus_tag="PL152_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004748395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase-peroxidase"
                     /protein_id="HQR49266.1"
                     /translation="MSNSTEGKCPFGHGAAGTTNRDWWPNQLPLDLLSQHSSLSDPMG
                     EDFDYAEEFKRLDYAGLKRDLAALMTDSQDWWPADFGHYGPLFVRMAWHSAGTYRTGD
                     GRGGGGRGQQRFAPLNSWPDNTSLDKARRLLWPIKQKYGRRISWADLMILAGNVALET
                     MGFKTFGFGGGRKDVWEPDQDVYWGTEKTWLAGDQRYSGKRDLENPLAAVQMGLIYVN
                     PEGPNGNPDPIAAAKDIRETFARMAMNDEETVALIAGGHTFGKTHGAGPATNVG"
     gene            complement(1020..2948)
                     /locus_tag="PL152_08015"
     CDS             complement(1020..2948)
                     /locus_tag="PL152_08015"
                     /inference="COORDINATES: protein motif:HMM:NF017591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF839 domain-containing protein"
                     /protein_id="HQR49267.1"
                     /translation="MSQPASLTRRSVLKALGATPVLPIAASFAGESPAANGHHGASAA
                     NRYVTYEFGSMAAPSLADPAQMATTYVASTLTKRYGRNHAETYSLGYETFFLTGEAVP
                     ATGGGTIVAGGYYDIDGNPILDATSPDQRQFFSDCPDGMSLIELGGSGRGRGRNRCNR
                     IFAVVQFEYVTRNVALQSMYGLLPSPIAVLTLDQDPRTGRLSLVSYHNVDTSSVRGLW
                     ISCGASRSPWNTHLSSEEYEPDATLATNAQLRAFSQNLFGDPARANPYDYGHLPEVTV
                     NPDGTGSIRKHYCLGRISHELVQVMPDERTVLMGDDATNGGLFMFIADRARDLSAGTL
                     YAAKWTQTSGTGPGAGRISWIRLGHASSDEIRALVDSGIRASDIMSVKTSDPQDSSYT
                     RIRFNGTNNWVKLVSGMEKAAAFLETHRYAALAGASLGFSKMEGTTVNAADKRAYSAM
                     SYIYKSMIDGTTDIKVQGPVAGAVYEHVLAGNQFDSEGRRIDSDWVSTYMSAPDGLVG
                     RDLAVRDALGNSADPDRIANPDNLKYSEAMRTLFIGEDSGNHVNNFLWAYNVDTGVLS
                     RLLSCPSGAESTGLQAVDDLNGYSYIMSNFQHPGDWESPLHDVVRPVLDPLEKANYNG
                     RYSAAVGYLAITGRVKDR"
     gene            3240..4253
                     /locus_tag="PL152_08020"
     CDS             3240..4253
                     /locus_tag="PL152_08020"
                     /inference="COORDINATES: protein motif:HMM:NF024989.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HQR49268.1"
                     /translation="MTTMRVAQVSRPDGPFEIVTRPVCQPSAGEVRVKVEACGVCHSD
                     AYAKRAAFPGIELPRVPGHEVIGRVDALGDGVVGFKVGDRVGVGWHGGQCGHCDPCRR
                     GDFFACQTFHRITGITTDGGYGEYVVAHQSALARVPAGLTAEEAAPLMCAGVTTFNSL
                     RNCGARAGDLVAVLGLGGLGHLAVQFAAKMGFRVAGIARGADKASFARELGAHHYIDS
                     LVQDPAAALREFGGARVILATVTNAEAMQACMGGLGPRGTFLVVGAVPALQVPPLQMI
                     GGNQSVRGWYSGTAIDSQDTLAFCALSGVRSMNEVYPLARVADAYEHMMSGRARFRVV
                     LKV"
     gene            complement(4306..>5235)
                     /locus_tag="PL152_08025"
     CDS             complement(4306..>5235)
                     /locus_tag="PL152_08025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49269.1"
                     /translation="RHEYRARIGNRLWLAMTAVAMTGCATPPPPPEPTSLPQTGDTVE
                     QIAARGYEVALVSALVADDHEYAYLVAHSTNSNFELVQVDGKTACGRTAALEPATQQQ
                     GSTDWQWLDLGSQPQPMTAARHWQEFTQWQWVAQPDGLRYLASQMRQACDLEADTEPQ
                     SLPSPWTRRVKDTPPPQSSSDDSVGEMLLHILIGVPVIVMAAPYVIAGETAALVVSAP
                     FVAAEHSANTRQIEQRKQMHLGLTRADTESLLGKPEAEFTLDPAPTKVLMYEAGESPQ
                     NYFVGFENDAIVWIHAQDEWLEHLAKRARKEKS"
BASE COUNT          766 a         1685 c         1866 g          918 t
ORIGIN      
        1 gtccgacgtt ggtcgccggg ccggcgccgt gcgtcttgcc gaaggtgtgg ccgccggcga
       61 tcagtgcgac ggtctcctcg tcgttcatcg ccatgcgcgc gaaggtttcg cggatgtcct
      121 tggccgccgc aatcgggtcg gggttgccgt tcgggccttc gggattgacg tagatcagtc
      181 ccatctgcac ggccgcaagg ggattctcga ggtcacgctt gccggaataa cgctggtcgc
      241 ccgcgagcca ggtcttctcg gtgccccagt acacgtcctg gtccggttcc cagacgtcct
      301 tgcgcccgcc gccgaagccg aaagtcttga aacccatcgt ttcgagcgcg acgttgccgg
      361 cgaggatcat gaggtcggcc catgagatgc ggcggccata cttctgcttg atcggccaca
      421 acagccgacg tgccttgtcg aggctggtgt tgtcgggcca gctgttcagc ggtgcgaagc
      481 gctgctggcc gcggccgccg ccaccgcggc cgtcgccggt gcggtaggtg ccggcgctgt
      541 gccacgccat gcggacgaac agcgggccgt agtgcccgaa gtccgccggc caccagtcct
      601 gcgaatcggt catgagcgcg gcgagatccc tcttcaggcc cgcgtagtcg agcctcttga
      661 actcctccgc gtagtcgaag tcctcgccca tcgggtcgga cagggaggag tgctgcgaga
      721 gcaggtcgag cggcagctgg ttcggccacc agtcacggtt ggtcgtgccg gcggcgccgt
      781 gtccaaacgg gcacttgcct tcagtcgagt tgctcatgtc tggctccacc tcgtgattga
      841 gtgggcctac gatacgcctc tccggcgatt gacgtgatcg ctggaaccga tcgtgacgat
      901 agacagcccc aggtgttcgg cagctgcctg gaacggcgcg gcgcagggtg tgtcgagcct
      961 ggaccccggc cgccggcccc ggcccacacg cggtgagccg gggccgcact tcgcgcgcct
     1021 caacgatcct tgacgcgacc cgtgatggcg agatagccga cggcggcact gtagcggccg
     1081 ttgtagttgg ccttctcgag cggatcgagc accggcctga ccacgtcgtg cagcggactc
     1141 tcccagtcac ccgggtgctg gaagttgctc atgatgtagc tgtagccgtt caggtcgtcg
     1201 accgcctgca ggccggtgga ctccgcgccc gacgggcagg agagcaggcg gctcagcacg
     1261 ccggtgtcga cgttgtaagc ccacaggaaa ttgttgacgt ggttgccgct gtcctcgccg
     1321 atgaacagcg tgcgcatggc ctcggagtac ttcaggttgt cgggattggc gatgcggtcg
     1381 gggtcggcgc tgttgccgag cgcgtcgcgc acggcaaggt cgcggccgac gagcccgtcc
     1441 ggcgcggaca tgtaggtcga cacccagtcg ctgtcgatgc gccgaccctc gctgtcgaac
     1501 tggttgccgg ccagcacgtg ctcgtagacc gcgcccgcga ccgggccctg caccttgatg
     1561 tcggtggtgc cgtcgatcat cgacttgtag atgtaggaca tcgccgagta cgcgcgcttg
     1621 tcggcggcgt tgaccgtggt gccctccatc ttcgagaagc ccagcgacgc cccggccagg
     1681 gccgcgtagc gatgcgtctc gaggaatgct gcggccttct ccatgccgga aaccagcttc
     1741 acccagttgt tggtgccgtt gaagcggatg cgggtgtacg acgagtcctg cggatcgctc
     1801 gtcttcacgc tcatgatgtc ggaggcgcgg atgccgctgt cgaccagcgc gcggatctcg
     1861 tcgctcgacg cgtgcccgag ccggatccac gaaatgcgac cggcgccggg gccggtgccg
     1921 gaggtctggg tccacttcgc cgcgtacagc gtgccggccg acaggtcgcg cgcgcgatcg
     1981 gcgatgaaca tgaacagccc accgttggtc gcatcgtcgc ccatcagaac cgtgcgttcg
     2041 tcgggcatca cctgcacgag ttcgtgcgag atgcgtccga ggcagtaatg cttgcggatg
     2101 ctgccggtcc cgtcgggatt gacggtgacc tcgggaaggt gaccgtagtc gtagggattg
     2161 gcgcgcgccg ggtcaccgaa gagattctgg ctgaaagcgc gcagctgcgc attggtggcg
     2221 agcgtcgcgt cgggctcgta ctcctcgctc gagaggtgcg tgttccacgg cgagcggctg
     2281 gcgccgcagc tgatccacag gccgcgcacg ctggacgtgt cgacgttgtg atagctcacg
     2341 agcgagagcc gtccggtgcg tggatcctgg tcgagcgtga gcacggcgat gggcgacggc
     2401 agcaggccgt acatggactg cagggcgaca ttgcgcgtca cgtattcgaa ctgcacgacg
     2461 gcgaagatcc ggttgcagcg attgcgcccg cgtccgcggc ccgatcctcc gagctcgatc
     2521 agcgacatgc cgtcggggca gtccgagaag aactggcgct ggtcgggcga ggtggcgtcg
     2581 agaatcggat tgccgtcgat gtcgtagtag ccgccggcga cgatcgtgcc gccacccgtc
     2641 gccgggacgg cctcacccgt gaggaagaac gtctcgtagc cgagcgagta ggtctcggcg
     2701 tggttgcgtc cgtagcgctt cgtcagcgtc gacgcgacgt acgtcgtggc catctgtgcc
     2761 gggtcggcga gcgacggcgc agccatcgag ccgaattcgt aggtgacgta gcgattggcg
     2821 gccgacgcgc catggtgacc attggcggcg ggcgactcgc cggcgaacga cgcggcaatg
     2881 ggcaggaccg gggtcgcacc gagcgccttg aggacgctgc ggcgcgtgag cgaagcgggc
     2941 tgggacatcg gggacctccg acggcatgaa tttcacgatt ggagaggtag aagtccgccg
     3001 cagcggaccg gcgctcaacg tagcgaagtc ttgtgacagt gccttgacga acggcggcgc
     3061 gacgagcgcg ttccgaacgc gatcgtcgcg gtcgcgccgg caacgaatcc gatggcgatg
     3121 aatggcagca ggtgcatcac gggatccgat tctgcgatcg gaatgcgaca ggaaagctac
     3181 aatcgatgcc ggcgcgtacc cggcccgctg atgccgctca ccgcagccag gagttcccca
     3241 tgacgacgat gcgtgttgcc caggtgtccc gtcccgacgg gccgttcgag atcgtcaccc
     3301 gcccggtctg ccagccgtcg gccggcgagg tgcgagtcaa ggtcgaggcc tgcggcgtct
     3361 gccacagcga tgcctacgcc aagcgcgcgg cgttcccggg catcgagctg ccgcgcgttc
     3421 ccggccacga ggtgatcggg cgcgtcgatg cgctgggcga cggcgtcgtc gggttcaagg
     3481 tcggggatcg cgtcggcgtc ggctggcacg gcggccagtg cggccactgc gacccgtgcc
     3541 ggcgcgggga cttcttcgcc tgccagacgt ttcaccgcat cacggggatc acgacggacg
     3601 gtggctacgg tgagtacgtc gtcgcgcacc agtccgcgct cgcgcgcgtg ccggccgggc
     3661 tcacggccga ggaggccgcg ccgctcatgt gtgccggcgt gaccacgttc aattcattgc
     3721 gcaactgcgg ggctcgcgcc ggcgacctcg tcgccgtgct cgggctcggc gggctcggac
     3781 acctcgcggt tcaattcgcg gcgaagatgg ggttccgcgt ggccggcatc gcgcgcggcg
     3841 ccgacaaggc gagcttcgct cgggaactcg gagctcatca ctacatcgac agcctggtgc
     3901 aggacccggc cgcggctttg cgcgagttcg gcggcgccag ggtgatcctc gcgaccgtga
     3961 cgaacgccga ggcgatgcag gcctgcatgg gcgggctcgg gccgcggggc acgttcctcg
     4021 tggtcggtgc ggtgcccgcg ctgcaggtgc cgcccctgca gatgatcggc ggcaaccagt
     4081 cggtgcgcgg ctggtactcg ggcacggcga tcgattcgca ggacacgctg gcgttctgcg
     4141 cgctgagcgg cgtgcgctcg atgaacgagg tgtatccgct ggcacgcgtc gccgacgcgt
     4201 acgagcacat gatgagtggc cgggcgcggt tccgggtcgt gctcaaggtg tagggcgcgt
     4261 cagcaaggct cggcacgccg ggacgggacg ctccgccggg tccgcctagc tcttttcttt
     4321 ccgggccctt ttcgccaggt gctcgagcca ctcgtcctgc gcgtggatcc agacgatcgc
     4381 gtcgttttcg aatccgacga aatagttctg cgggctctcg ccggcctcgt acatgagcac
     4441 cttggttggc gcagggtcga gcgtgaactc tgcttcgggt ttgccgagca gcgattcggt
     4501 gtcggcgcgg gtcagcccga gatgcatctg cttgcgctgc tcgatttgcc tggtgttcgc
     4561 ggagtgctcg gccgcgacga acggagcgct caccacgagc gcagccgtct cgcccgcaat
     4621 gacgtagggc gccgccatga cgatcacggg cactccgatg agtatgtgca acaacatctc
     4681 tccgaccgag tcgtccgaac tcgactgcgg cggcggggtg tctttcacgc gcctggtcca
     4741 cggagacggc agcgactgcg gttccgtgtc cgcttcgagg tcgcatgcct gccgcatctg
     4801 gctcgcgagg tagcgcaggc cgtcgggttg cgcgacccac tgccactggg tgaactcctg
     4861 ccagtgccgg gccgccgtca tgggctgggg ctgggacccc agatccagcc actgccagtc
     4921 cgtcgatccc tgctgctgtg tggccggctc cagtgccgcc gtacggccgc aggctgtctt
     4981 gccgtcgacc tggaccagtt cgaaattcga gttcgtcgag tgcgcgacga ggtacgcgta
     5041 ctcgtgatcg tccgcgacga gtgcagatac gagcgcgacc tcgtacccgc gtgccgcaat
     5101 ctgctcgacg gtgtcacctg tctgcggcag tgacgttggc tcgggcggag gcggtggcgt
     5161 cgcgcacccg gtcatggcca ccgcggtcat ggcgagccac agccggttcc cgatgcgggc
     5221 ccggtactcg tgtcg
//
LOCUS       DAOMFD010000135         5226 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6452_length_5226_cov_33.508799, whole genome shotgun sequence.
ACCESSION   DAOMFD010000135 DAOMFD010000000
VERSION     DAOMFD010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6452_length_5226_cov_33.508799"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(2..220)
                     /gene="infA"
                     /locus_tag="PL152_08030"
     CDS             complement(2..220)
                     /gene="infA"
                     /locus_tag="PL152_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HQR49270.1"
                     /translation="MAKEDAIQMEGRVVETLPNTTFRVQLQNGHVVTAHISGKMRKNY
                     IRILTGDTVTVEMTPYDLTKGRIVYRGR"
     gene            complement(289..975)
                     /gene="aat"
                     /locus_tag="PL152_08035"
     CDS             complement(289..975)
                     /gene="aat"
                     /locus_tag="PL152_08035"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HQR49271.1"
                     /translation="MIPILRPDSPPDAFPPVASALDEPDGLLCAGGDLSTARLIAAYR
                     RGIFPWYSEGQPILWWSPDPRTVLIPAEFKVSRSLAKSLRNRGFETTVDRAFDAVMAG
                     CADRRLRPEGTWISPDMQAAYLRLHELGLAHSIETWLDGRLVGGLYGVSLGRVVFGES
                     MFSLERDASKVALKRLVDVALARHVELIDCQVASLHLESLGARAVPRSEFVGRLAGAI
                     PDVVAPDRWD"
     gene            complement(1028..2176)
                     /locus_tag="PL152_08040"
     CDS             complement(1028..2176)
                     /locus_tag="PL152_08040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR49272.1"
                     /translation="MRVQAETLERITDVPGAEWDALDIAGNPFLRHGFLAALESTGCV
                     GMESGWMPRHLVLRDPQGRLAGAVPRYFKTHSWGEFVFDWNWAQSALRAGLPYYPKLV
                     AAVPFTPVTGPRLLATPGSAAATRGELARQLVESAQRDAAQVTQVNFTTADDQASLLA
                     AGFLARHDCRFLWRNRGYRDFDDFLDTFRAEKRKKLRRERRRVAEAGVTFDTLAGEAI
                     DPGLWQVIFGFSEQTFLRHGNPHYLTADFLQLVSQKLPGVVMVKLARIAGEPVAAAIF
                     FQDGTSLYGRYWGAARHVDCLHFEACYHQGIEWCIERGLAEFDPGTQGEHKLARGFEP
                     TLTRSAHWFAHPGLSAAIGRYLERERAAVDDYMAAAASHLPFQRDAPA"
     gene            complement(2233..3198)
                     /gene="trxB"
                     /locus_tag="PL152_08045"
     CDS             complement(2233..3198)
                     /gene="trxB"
                     /locus_tag="PL152_08045"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HQR49273.1"
                     /translation="MSGPRHSRLIILGSGPAGYAAAVYAARANRKPLLLTGMEQGGQL
                     MTTTEVDNWPGDPHGLTGPGLMERMREHAARFDTEIVMDHVNEVDLTKRPMVLKGDRG
                     EYTCDALIIATGASARYLGLESEQAFRGRGVSACATCDGFFFRGQRVAVIGGGNTAVE
                     EALYLSNIASHVTLVHRRDKLRSEKILQDRLFERQKAGVVDIAWNHEVVDVLGDDSGV
                     TGLRIKATDGSGSRDLDVAGVFIAVGHTPNTQIFEGQLEMKGGYIAIKSGTEGGATAT
                     SVAGVFAAGDVADHVYRQAITSAGTGCMAALDADKYLEQFDAGHS"
     gene            3438..>5226
                     /locus_tag="PL152_08050"
     CDS             3438..>5226
                     /locus_tag="PL152_08050"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HQR49274.1"
                     /translation="MARGLRESALWVFGALALILLVALASYDRADPAFSSTGQSGPVT
                     NLIGPFGAWLSDLFFVLFGAPAYLFPIVLGYAGWTLFQERRSAEPIDRRTSLLRGTGF
                     VLALASSCGLATLHFSGGSLPYTAGGQVGALVGDGLAHSLSLLGATLLLLAVWLGSVS
                     LCTGISWLAVMDRIGRAVLRGIAWTRERSATARDVKAGREVKQARQEVVREEQKKAAE
                     RKPPKIEAVAPRVEKSERVEKEKQVSMFERPGATELPALSLLDDAPQRQGGYSPEALE
                     AMSRLVELKLRDFGVEAEVVEVHPGPVITRFELRPAPGVKVAQISHLAKDLARALSAV
                     SVRVVEVIPGKSTMGLEIPNESREIVTLGEIIKSKAYDEMASALALVLGKDIGGNPVV
                     ADLARMPHLLVGGTTGSGKSTAINAMVLSLLYKSSAEQVRLIMIDPKMLELSVYEGIP
                     HLMVPVVTDMKQAANALRWCVAEMERRYLLMAHAGVRNLAGYNRKVKDLADAGTPLRD
                     PIEMKRLPPGGDPESVPFVEPLPFIVVVIDELADLMMIVGKKVEELIARLAQKARASG
                     IHLILATQRPSVDVITGLIKANIPTRIAFQ"
BASE COUNT          795 a         1788 c         1826 g          817 t
ORIGIN      
        1 ttcagcgtcc ccggtagacg atgcgtccct tggtgaggtc gtagggggtc atctccaccg
       61 tcacggtgtc gcccgtcagg atgcggatgt agttcttgcg catcttgccc gagatgtgcg
      121 cggtcacgac gtggccgttc tgcagctgga cacgaaaggt cgtgttcggc agggtctcga
      181 ccacccggcc ttccatctgg atcgcgtctt ccttcgccat gcaccctctc tgcggccctg
      241 cgagccgcgc gaattctgca taaaggcctg ccgcaatgca atgcaacgtc agtcccagcg
      301 gtcgggtgcg acgacgtccg gaatcgcgcc cgccagccgg ccgacgaact ccgatcgcgg
      361 gacggcgcgg gcgccgaggc tttcgaggtg caggctcgcg acctggcagt cgatgagctc
      421 gacgtgccgc gcgagggcga cgtccaccag tcgcttgagt gccaccttgg acgcgtcgcg
      481 ctccaggctg aacatggact cgccgaagac gaccctgccg agcgacacgc cgtacagccc
      541 gccgaccagc cggccgtcga gccaggtctc gatcgagtgc gcgagcccta gctcatgcag
      601 cctgaggtac gcggcctgca tgtccgggga gatccacgtg ccttccggcc gcagccgccg
      661 gtccgcacag cccgccatca ctgcatcgaa ggcgcggtcc acggtggtct cgaacccgcg
      721 attgcgcagc gacttggcca ggctgcggga caccttgaac tcggcaggga tcagcaccgt
      781 gcgcgggtcg ggcgaccacc acaggatcgg ttgcccctcc gagtaccacg ggaagatgcc
      841 gcgccggtac gccgcgatca gccgcgcggt cgacaggtcg ccgcccgcgc acagcaggcc
      901 gtcgggctcg tcgagcgcgg aagcgacggg cggaaatgcg tccggcggac tgtcggggcg
      961 caggatcggg atcacgtggt cgcgcctccg caatggcgga ccacctcggc aatgtattcg
     1021 gcgctggtca cgcgggcgcg tcgcgctgga acggcaggtg cgaggcggcc gcggccatgt
     1081 agtcgtcgac cgccgcgcgc tcgcgttcga ggtaacgacc gatcgctgcc gagagcccgg
     1141 gatgcgcgaa ccagtgcgcc gagcgggtca gcgtgggctc gaagccgcgc gccagcttgt
     1201 gctcgccctg cgtgcccggg tcgaactcgg cgaggccgcg ctcgatgcac cactcgatgc
     1261 cctggtggta gcacgcctcg aagtgcaggc agtcgacatg gcgtgccgcg ccccagtagc
     1321 ggccgtagag cgacgtgccg tcctggaaga agatggcggc agcgaccggc tccccggcga
     1381 tgcgcgcgag cttgaccatg accaccccgg gcagcttctg cgacacgagt tgcaggaagt
     1441 cggcagtcag gtaatgcgga ttgccgtggc gcaggaatgt ctgctccgag aagccgaaaa
     1501 tcacctgcca caggccggga tcgatggctt cgccggcgag ggtatcgaag gtcacaccgg
     1561 cctcggccac gcgccggcgc tcgcgccgca acttcttgcg cttctcggcg cgaaacgtgt
     1621 cgaggaagtc gtcgaagtcg cgatagccgc gattgcgcca caggaagcga cagtcgtggc
     1681 gggccaggaa cccggcggcc agcagcgatg cctggtcgtc ggccgtcgtg aagttcacct
     1741 gcgtcacctg ggcggcgtcg cgctgggccg actcgaccag ctgtcgcgca agttcgccgc
     1801 gcgtcgcggc cgcggagccg ggtgtcgcaa gcaggcgcgg gcccgtcacc ggcgtgaacg
     1861 gcacggcggc caccagcttg gggtagtagg ggaggcccgc cctcagcgcc gactgggccc
     1921 agttccagtc gaacacgaat tcaccccagg aatgcgtctt gaagtagcgg ggaaccgctc
     1981 ccgcgagccg gccctgcggg tcgcgcagca ccaggtgccg cggcatccag ccggactcca
     2041 ttccgacgca acccgtcgac tccagcgccg cgaggaatcc gtggcgcagg aacggattgc
     2101 cagcgatgtc gagggcatcc cactcggcgc ccggcacgtc ggtgatccgt tcgagggttt
     2161 cggcctgcac gcgcatcgcg cggctgctcc gcggatcggg cctggtcccg atggggtccc
     2221 gggccactgc cttcaggagt ggccggcgtc gaactgttcg aggtacttgt cggcgtcgag
     2281 cgccgccatg cacccggtgc cggccgacgt gattgcctgg cgatacacgt ggtccgcgac
     2341 gtcgcccgcg gcgaagaccc cggccacgct cgtcgccgtc gcgccgccct ccgtacccga
     2401 tttgatggcg atgtagccgc ccttcatctc gagctgccct tcgaagatct gcgtgttggg
     2461 cgtgtgcccg accgcgatga agacacccgc gacgtccagg tcgcggctgc ccgagccgtc
     2521 ggtcgccttg atgcgcaggc cggtgacccc cgaatcgtcg ccgaggacgt cgacgacctc
     2581 gtgattccag gcgatgtcca cgaccccggc cttctgccgc tcgaacagcc ggtcctgaag
     2641 gatcttctcc gagcgcagct tgtcgcggcg gtgcactagc gtcacgtgcg aggcgatgtt
     2701 ggagaggtag agcgcctcct cgaccgccgt gttgccgcca ccgatgacgg cgacgcgctg
     2761 gccgcggaag aagaatccgt cgcaggtcgc gcaggccgac acaccgcggc cgcggaatgc
     2821 ctgctccgat tcgagcccga ggtagcgcgc gctcgcgccg gtcgcgatga tgagcgcgtc
     2881 gcacgtgtac tcgccgcggt cacccttcag gaccatcggt ctcttcgtga ggtccacctc
     2941 gttcacgtgg tccatcacga tctcggtgtc gaagcgcgcc gcgtgctccc gcatccgctc
     3001 catgaggccc ggtcccgtga gcccgtgcgg atcgcccggc cagttgtcca cctcggtggt
     3061 cgtcatgagc tggccaccct gttccatccc ggtgagcagc agcggcttgc gattcgcgcg
     3121 cgccgcgtag acggccgcgg cgtagccggc aggcccggac ccgaggatga tcagccgcga
     3181 gtgacgggga ccgctcatgt atacttcctc gcgcttcgag cgccgtgaac ggatgggggc
     3241 gcggcatggt aatgaactac ccgtcccgcg gcaaagcacc ccccgccggg cgctccgggg
     3301 tcgcctgccg aggcccgctg gcacccgccg cgccatcccg gtaatcttcg caacttcata
     3361 cgcttacgcg aaagtattga gctttcaggt gatcagtggc ggcgaggact gaatcgaaac
     3421 acgaagcggc gtcgagcgtg gcccgcggcc tgcgcgaaag cgcgctgtgg gtgttcggcg
     3481 ccctggcgct gatcctgctc gtggcgcttg cgtcgtacga ccgggccgac ccggcatttt
     3541 cctccaccgg gcagtcgggc ccggtgacca acctgatcgg gccattcggg gcgtggctgt
     3601 cggacctgtt cttcgtgctg ttcggcgcac cggcctacct gttcccgatc gtgctcggct
     3661 acgccggctg gacgctgttc caggagcgtc gcagcgcgga gcccatcgac cggcgcacgt
     3721 cccttctgcg cggcacgggt ttcgtgctcg cactggcgag cagctgcgga ctcgcgaccc
     3781 tgcatttctc gggcggctcg ctgccctaca cggccggcgg ccaggtcggc gcgctggtcg
     3841 gcgacgggct cgcccactcg ctcagcctgc tcggcgcgac gctgctgctg ctggccgtgt
     3901 ggctcggttc cgtctcgttg tgcaccggca tctcctggct cgccgtcatg gatcgcatcg
     3961 gccgcgcggt gctgcgcggc atcgcgtgga ccagggaacg cagcgcgacc gcgcgcgacg
     4021 tgaaggccgg ccgcgaggtc aagcaggcgc ggcaggaggt cgtgcgcgag gagcagaaga
     4081 aggccgcgga gcgcaagccg ccgaagatcg aggcagtggc gccgagggtc gagaagagcg
     4141 agcgcgtcga gaaggaaaag caggtctcga tgttcgagag gccgggagcg acggagctgc
     4201 cggcgttgtc gctgctcgat gacgcaccac agcgccaggg cggctactcg ccggaggcgc
     4261 tggaggcgat gtcccggctg gtcgaactca agctgcgcga tttcggcgtc gaggccgagg
     4321 tggtcgaggt gcacccgggg ccggtcatca cgcgcttcga gcttcgtccc gcgcccggcg
     4381 tcaaggtcgc gcagatttcg cacctcgcca aggacctcgc gcgcgcgctg tcggcggtga
     4441 gcgtgcgcgt cgtggaagtg atcccgggca agtccaccat gggcctcgag atcccgaacg
     4501 agtcgcgcga gatcgtgacg ctcggcgaga tcatcaagtc gaaggcctac gacgagatgg
     4561 cctcggcgct cgcgctggtg ctcggcaagg acatcggcgg caacccggtc gtggcggacc
     4621 tcgcgcgcat gccgcacctg ctggtcggcg gcacgacggg ctcgggcaag tcgaccgcca
     4681 tcaacgcgat ggtgctgtcg ctgctctaca agtcgtccgc cgagcaggtc cggctgatca
     4741 tgatcgaccc gaagatgctg gagctctcgg tgtacgaggg catcccgcac ctcatggtcc
     4801 cggtcgtgac cgacatgaag caggcggcca acgcgctgcg ctggtgcgtg gcggagatgg
     4861 agcgccgcta cctgctcatg gcgcatgccg gcgtgcgcaa tctcgccggc tacaaccgca
     4921 aggtgaagga cctcgccgac gccggcacgc cgctgcggga cccgatcgag atgaagcgcc
     4981 tgccgccggg cggcgatccc gagtcggtcc cgttcgtgga accgctgccg ttcatcgtcg
     5041 tggtcatcga cgagctcgcc gacctgatga tgatcgtcgg caagaaggtc gaggaactga
     5101 tcgcccggct ggcgcagaag gcgcgtgcct cgggcatcca cctgatcctg gccacgcagc
     5161 ggccgtcggt cgacgtgatc acgggcctca tcaaggcgaa catcccgacg cgcatcgcgt
     5221 tccagg
//
LOCUS       DAOMFD010000136         5204 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6504_length_5204_cov_32.322393, whole genome shotgun sequence.
ACCESSION   DAOMFD010000136 DAOMFD010000000
VERSION     DAOMFD010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5204)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5204
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6504_length_5204_cov_32.322393"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..639
                     /locus_tag="PL152_08055"
     CDS             <1..639
                     /locus_tag="PL152_08055"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HQR49275.1"
                     /translation="VYYVKGAFLDADKAFEVISGMRTVELRIINKAINTLVLARALAR
                     HPAINVNCSAVEGNENYALREKQMFLGLPAPLFTIDLETPLGAGIGREPFQRFFDCLE
                     PAFGLQVTLGQGNTVVVCPALTSHSELSPEALREAGIAPTTIRIAVGEEDPRALLAHL
                     MRAAELAIEPAAPGFLAHFASPAEIDRLYEDNYADVHRRYAASRPRMAQLMA"
     gene            complement(640..1740)
                     /locus_tag="PL152_08060"
     CDS             complement(640..1740)
                     /locus_tag="PL152_08060"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49276.1"
                     /translation="MPLASLPPGCEPACHGCRHRLRTADESIAQKTAYLAQALHPWAD
                     RIQPAAVPVESERLGYRDRVTLNAGWSAEHGWRVGLMRRDELIPIPDCPVHTERVRAL
                     VHLLMRQLPPVDRLPLAYLHVAGAQATLIVKARNLDDEVLAPLLGCIVGTGIEGLWLH
                     LHPAAGRRLFARNGWRLLWGAAESRGPGGLLHGPTAFMQPLPGLYVAAVDEARRHLAP
                     GASVAVLDLYCGIGATLREWTRAGSPALGVELSGEAVACSIRNAPAATVLRGTCAQRL
                     PQIRGWWRDVGGEGVAFVNPPRSGLEAGVTQALTSDLRPARIAYLSCSAGTLARDLMA
                     FEAAGFGVRSIRPYDFFPNTHHVEALALVERR"
     gene            complement(1841..1966)
                     /locus_tag="PL152_08065"
     CDS             complement(1841..1966)
                     /locus_tag="PL152_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49277.1"
                     /translation="MMSTCSNSDRVLSKVLAGLAITVTFVFAAVVHAVFMAQPFA"
     gene            complement(2142..2849)
                     /locus_tag="PL152_08070"
     CDS             complement(2142..2849)
                     /locus_tag="PL152_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49278.1"
                     /translation="MKRIITGLSAMMVVALAPQYAGAYGHANRFGGSTEHEAGVGTEH
                     TNAWGGNTEHAFGGGTEHNNVYGGHTEGEAGEGASHTNMYGGTTSGAYGEGAYHSTPY
                     GGTAYVPPAGYAYDYHPPAEVNYYGAGCYNCGGWDAAGAAVAGAVVGATVATAAESSS
                     TTTATTNAYNAGVATGAAEASGAPGSYTMGAMYPSLPAGCVEPTVKGIAYYLCGNTWF
                     QPNYGSGGVYYKVVPAP"
     gene            complement(2865..3713)
                     /locus_tag="PL152_08075"
     CDS             complement(2865..3713)
                     /locus_tag="PL152_08075"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HQR49279.1"
                     /translation="MIKRISLAALIAVAMLTIAGAPSVRAGDAAATPGATPSTSGNLA
                     PAIELEPKALNILKGACDKLGAARTMAFKAVVSEESPSRLGPPLAYYTTSEVLLQRPD
                     KLRVVSPGDGPRSEFYYDGKSMVAYSPVENMVARASAPATIDAMLEAAFKTAQIYFPY
                     TDLLVTDPYKGLSDGLRVAFYVGQSDSVGGVKTHIIAYANDHAFVQAWIGVDDRMPRR
                     LQAVFRSDPLQLRHSVNLSDWKLDAAVPAGAFTPPEKAKKALPIAFGNPAAMPAGDRS
                     SAAKKQ"
     gene            complement(3806..4888)
                     /locus_tag="PL152_08080"
     CDS             complement(3806..4888)
                     /locus_tag="PL152_08080"
                     /inference="COORDINATES: protein motif:HMM:NF016974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HQR49280.1"
                     /translation="MHAGQADAHVFVGLALACQRLGDATGLDDAVGRALALDPRNLHA
                     LLIRGDRSAATGDLRGSAAFYGAAINVASQEKNLPPALASAVQRAAESRERINEHVAR
                     QLAAQLAAAGYDPARSSRRFSHSLDLMHGRRRIHYQQPRAYYFPELPQVQFYPRETCP
                     WLAGLEAATDDICAELAGVLERSRGFVPYLQSHPGVPETGDRRLLDNLDWSAFFLYRN
                     GAIVEENAAQCPKTMAAIERLPLPRVRGRDPMALFSVLKPGVRIPPHCGYLNTRLICH
                     LPLIVPPGCHFRVGNEQRQWRKGEAWVFDDSIEHEAWNSSHETRVILLVDVWRPELSG
                     EERELVAALLEAMDAVGPAAKVEWTA"
BASE COUNT          809 a         1812 c         1761 g          822 t
ORIGIN      
        1 gtgtactacg tgaagggcgc gttcctcgac gccgacaagg cgttcgaggt gatcagcggc
       61 atgcgcaccg tcgagctgcg catcatcaac aaggccatca acacgctcgt gctggcgcgt
      121 gcgcttgcga ggcacccggc catcaacgtg aactgctccg cagtggaggg caacgagaac
      181 tacgcgctgc gcgagaagca gatgttcctg ggcctgccgg ctcccctgtt caccatcgac
      241 ctggagacgc ccctgggggc tggcatcggc cgcgagccgt tccagcgctt cttcgactgc
      301 ctcgagccgg ccttcggcct gcaggtgacg ctcggccagg gcaacaccgt cgtggtctgc
      361 cctgccctca ccagtcactc ggaactctcg cccgaagcgc tgcgcgaagc cggcatcgcg
      421 ccgacgacca tccgcatcgc ggtcggcgag gaagacccgc gcgcgctcct ggcgcacctg
      481 atgcgcgccg ccgaactcgc gatcgagccg gcggccccgg gatttctcgc gcactttgcg
      541 tcgccggcgg agatcgaccg gctgtacgag gacaactacg cggacgtcca ccgccgctat
      601 gctgcgagcc ggccacgcat ggcgcaactg atggcgtgat cagcggcgct cgacgagtgc
      661 cagcgcctcg acgtgatgcg tgttcgggaa gaagtcgtac ggccggatgg accgcacgcc
      721 gaagcccgcg gcctcaaagg ccatgaggtc ccgagccagc gtacccgcgc tgcaagacag
      781 gtatgcgatg cgcgcgggtc gcaggtccga cgtcagcgcc tgtgtcaccc cggcctcgag
      841 acccgaccgc ggaggattca cgaaggcgac gccctcgccg ccgacgtccc gccaccaccc
      901 gcggatctgc ggaagccgct gggcacaagt gccgcgcaga acggttgccg cgggcgcatt
      961 gcgaatggag cacgcgaccg cctcgccgga gagctcgacg ccgagcgccg ggctgcccgc
     1021 tcgagtccat tcacgcagcg tcgcgccgat tccgcagtac aggtcgagca ccgcaaccga
     1081 cgcgccgggc gcgagatggc gccgggcctc gtcgaccgcg gcgacgtaga gtccaggcag
     1141 cggctgcatg aaggcggtcg gtccgtgcag caggccaccc ggcccccgag actccgccgc
     1201 accccacaac aggcgccagc catttcttgc aaacaggcgg cgtcccgccg ctggatgcag
     1261 gtgcagccac agaccttcga tcccggttcc gacgatgcaa ccgaggagcg gcgcgagaac
     1321 ctcatcgtcg agattgcgcg ccttgacgat cagcgtggcc tgcgcaccgg ccacgtggag
     1381 atacgcgagt ggcagccggt cgaccggggg cagttgtcgc atcagcaggt gcaccagcgc
     1441 gcgcacgcgc tcggtgtgga ccggacaatc ggggatgggg atcagttcgt cgcggcgcat
     1501 gaggccgacg cgccagccgt gctccgccga ccatccggcg ttgagcgtca cccggtcgcg
     1561 atagccgagg cgttctgact cgaccggtac cgcggccggc tggattcggt cagcccatgg
     1621 gtgcagggcc tgggcgaggt aggcggtctt ctgggcgatg ctctcgtcgg ctgtccgcag
     1681 ccggtgcctg cagccgtggc aggcgggttc acatccaggg ggcagcgacg ctagtggcat
     1741 cgggcgaccg cccgttcccc tgcctccggc tgccccggtg ccccggaagg cgcgccactc
     1801 gttgcggcgt caccctgcca ggaccccgtg gcgtgggcgc tcaggcaaac ggctgggcca
     1861 tgaagaccgc gtgcacgacc gccgcgaaaa cgaaggtcac cgtgatcgcg aggccggcga
     1921 gcaccttgct gaggactcgg tcggaattgc tgcaggtgga catcatggct ggatcctccg
     1981 gttgaactcg gggccaacga tagtccgcca gattggtcag ataaatagaa tacttcagga
     2041 aactccgttc acgaattcgg aataatcgcc tggccagttc ccactggccg cgtcggctac
     2101 cacccgacgg acaccgggtc cgccgggtgg agccaagcgg ttcaaggcgc cggcacgacc
     2161 ttgtagtaca cgcccccgga accgtagttg ggttggaacc aggtgttacc gcacaggtag
     2221 taggcgatgc ccttcaccgt gggctcgacg cagcctgccg gcagcgacgg atacatcgcc
     2281 cccatcgtgt aggacccggg cgcgcccgac gcctccgcag caccagtggc gacccccgcg
     2341 ttgtaggcgt tggtcgttgc cgtcgtcgtg ctggaggatt ccgccgcggt ggcgacggtt
     2401 gcgccgacga ccgcaccggc tacggccgcg cctgccgcgt cccacccgcc gcagttgtag
     2461 cagccggcac cgtagtagtt cacctcggcg ggcgggtgat agtcatacgc ataaccggcc
     2521 ggcggcacgt acgccgtgcc tccgtacggt gtcgaatggt aggcaccctc gccgtacgcg
     2581 cccgacgtcg tgccgccgta catgttggtg tggctggcgc cttcgccggc ctcgccttcg
     2641 gtgtggccac cgtagacgtt gttgtgttcc gtgccgccgc cgaaggcgtg ttccgtgttg
     2701 ccgccccacg cgttggtgtg ctcggttccc acgcccgctt cgtgctcggt gctgccaccg
     2761 aagcggttgg cgtggccata ggcgcccgcg tactgcggcg cgagcgctac gaccatcatc
     2821 gccgacagtc ccgtgatgat tctcttcatg gcggtctcct taggtcactg cttcttggcg
     2881 gccgacgacc ggtccccggc aggcatcgct gccggattcc cgaacgcgat cggcaacgcc
     2941 ttcttcgcct tctcgggcgg cgtgaatgcg ccagccggga ccgctgcgtc cagcttccag
     3001 tccgacaggt tcaccgagtg gcgcaactgc agcggatcgc tgcggaacac ggcctgcagc
     3061 cggcgcggca tgcggtcgtc gacgccgatc caggcctgca cgaatgcgtg gtcgttggcg
     3121 taagcgatga tgtgcgtctt cacgccgccg acggagtccg actgcccgac gtagaacgcg
     3181 acccgcagcc cgtcggacag gcccttgtac gggtcggtca ccagcaggtc cgtgtacggg
     3241 aaatagatct gggccgtctt gaatgctgct tcgagcatgg catcgatcgt tgccggcgcg
     3301 gaagcgcggg ccaccatgtt ctcgacgggc gagtacgcga ccatgctctt gccgtcgtag
     3361 tagaactcgc tgcgcggccc gtcaccggga ctcacgacgc gcagcttgtc cggccgctgg
     3421 agcagcacct cggaggtcgt gtagtaagcg agcggcggcc cgaggcggct cggactctcc
     3481 tcgctcacca ccgccttgaa cgccatggtc ctggccgctc cgagtttgtc gcaggcgccc
     3541 ttcaggatgt tgagcgcttt cggctccagc tcgatcgccg gtgccaggtt gcccgaggtg
     3601 gatggcgtcg cgcccggcgt tgccgcggca tctccagcac gcaccgaagg tgcgcccgcg
     3661 atcgtcagca tcgccaccgc aatcagcgcg gcgagcgaaa tcctcttgat catgcgttga
     3721 tcctcgtgtt gttggtcttg aaggctgtcc ggccgcggac acggctcgac tggacaaatc
     3781 atagcgcgtc ccggcgtcac ctgcgttacg cggtccactc gactttcgcc gccgggccga
     3841 ccgcgtccat ggcctcgagc aaagccgcga cgagttcgcg ttcctcgcca ctcagctccg
     3901 gtcgccagac gtccaccagc aggatgacgc gcgtctcgtg gctcgaattc cacgcctcgt
     3961 gctcgatcga gtcgtcgaag acccacgcct cgcccttccg ccactggcgc tgctcgttgc
     4021 cgacccggaa atgacagccg ggcggaacga tcagtggcag gtggcagatc agccgcgtgt
     4081 tcaggtaccc gcagtgcggc ggaatcctta cgccaggctt gagcacggag aacagcgcca
     4141 tcgggtcgcg gccgcgaacc cgcgggagcg gcagccgctc gatcgccgcc atcgtcttcg
     4201 ggcactgtgc cgcgttctcc tcgacgatcg cgccgttccg atagaggaag aacgcactcc
     4261 agtcgaggtt gtcgagcagc cggcggtcgc cggtctcggg aacaccgggg tgcgactgca
     4321 ggtagggcac gaatccgcgg ctgcgttcga gcacgccggc gagctccgcg cagatgtcgt
     4381 ccgtcgcagc ttcgagcccg gccagccagg ggcacgtctc gcgcgggtag aactgcacct
     4441 gcggaagctc gggaaaatag tatgcgcgcg gctgctggta atggatgcgc ctgcggccat
     4501 gcatgaggtc gagcgaatgg ctgaagcggc ggctcgagcg cgccgggtca tagcccgcgg
     4561 cggcgagctg cgccgcgagc tgccgcgcga cgtgctcgtt gattcgctcc cgtgactccg
     4621 cggcgcgctg cacggcggag gccagcgcgg gcggcaggtt cttctcctgc gacgcgacat
     4681 tgatcgcggc gccgtagaac gcggccgagc cgcgcaggtc ccccgtcgcg gccgagcgat
     4741 cgccgcggat cagcagcgcg tgcaggttgc gcggatcgag cgcgagagcc cttccgacgg
     4801 cgtcgtcaag tcccgtcgcg tcgcccagcc tctggcaggc aagcgccagg ccgacgaaca
     4861 cgtgcgcatc ggcctgcccg gcgtgcacga tcgactcgaa ttgcccgcga gcggcctcgg
     4921 cgtcgccggc gtcgagcgcg gcccggccgg ccgcgaatgc agcattcctg tcgatgggag
     4981 aaaccgtccc cggggtgccg tcgaggaccg gatcatgacg cccgcccggc catgcggcaa
     5041 cccgggccga ttgacaccga ggccccatgc gacaaggatg cgaccttgtt cccgacaccg
     5101 tccctgggcg ccggcagacc ctgccccgct tcgcccggga cattcgaggc cgagcgatgt
     5161 tcgagcgcca tgggcgcgtc tgcgccgttc tattgaccgt ggca
//
LOCUS       DAOMFD010000137         5202 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6511_length_5202_cov_32.532349, whole genome shotgun sequence.
ACCESSION   DAOMFD010000137 DAOMFD010000000
VERSION     DAOMFD010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5202)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5202
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6511_length_5202_cov_32.532349"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3893)
                     /gene="purL"
                     /locus_tag="PL152_08085"
                     /gene_synonym="purI"
     CDS             complement(<1..3893)
                     /gene="purL"
                     /locus_tag="PL152_08085"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HQR49281.1"
                     /translation="MLTLSGAPAVSDFRLAKLLSSIRERTREVERLDSRHVHFVDVER
                     PLDAREAGVLESLLRYGPTVSAGAPQGELLLVVPRIGTVSPWSSKATDIAHVCGLAAV
                     RRIERGVAYYLGASRRLADEDWQAAAAVVHDRMTEAVLRDTAAAAALFEHAAPKPLST
                     VPLVAQGRAALVSANAELGLALSDDEIDYLVDAFRSLGRDPTDVELMMFAQANSEHCR
                     HKIFNADWIVDGSRGPKSLFGMIRNTHARNPRGVLSAYRDNAAVIEGWPGRRWFAQPG
                     GGAYAGADEPIDILMKVETHNHPTAISPFPGAATGSGGEIRDEGATGRGSKPKAGLVG
                     FSVSNLRIPGAVQPWEQDLGKPDRIVSALSIMLEGPIGAASFNNEFGRPGICGYFRTF
                     EQRVEAEGRSPLRGYHKPIMIAGGLGNIRREHVEKATIVAGAKIVVLGGPAMLIGLGG
                     GAASSVGAGASSADLDFASVQRGNPEIQRRAQEVIDACWALGDANPILLIHDVGAGGL
                     SNAVPESVAHGGCGGRVDLRAVPSDEPGMSPLEIWCNEAQERYVLIVAAGHMERFAAF
                     CARERCPYAVIGEVTSDGTLRVADPLFGNEPVGMRLETLLGKPPRMTRDVRRLRGASD
                     DFDGRDVDVRDAAFRLLRLPTIADKTFLIAIGDRSVGGMISRDPLVGPWQVPVSDVAV
                     TVSDYFSTRGEAMAMGERTPLALLDPAAAARMAVAEAVTNIAAADIARIEDIRLSANW
                     MAACGEPGEDADLYDAVRAVGEELCPALGIAIPVGKDSLSMKTAWQGSDGPRKVVAPV
                     SLIVSAFAPAQDVRRTLTPELRTDRGDTRLLLVDLGLGKDRLGGSCVAQVYGRVGSVA
                     PDCDDPAALRAFFSAITSLRDAGRILAYHDRSDGGLFVTLCEMAFAGRCGIEADLIAG
                     EGGAAVVSAMFAEELGAVLQVEAAAVAQVVAEFSRAGLADSVHVIGRVTSDDAVVIRS
                     RGRTILQATRTELRRAWSETTFRMQSLRDNPECAAEEYARATDPADPGLHARLTFDPA
                     ADTAAQFLVKGTRPPVAILREQGVNSQLEMAAAFHRAGFAPVDVHMTDLIAGRTRLDD
                     YVGLVACGGFSYGDVLGAGEGWAKSILFNPMLRAQFEAFFARTDSFTLGVCNGCQMMA
                     ALKDLVPGAAHWPRFVRNRSEQFEGRVGLVEVLPSPSVFFAGMAGSVMPIAVAHGEGR
                     AEFATAEAMRACVGSGLVSLRFVDNHGRPAEAYPANPNGSPSGITGLTSSDGRATIVM
                     PHPERVYRTVQNSWHPAEWGEDSGWMRMFRNARAWV"
     gene            complement(3976..4314)
                     /locus_tag="PL152_08090"
     CDS             complement(3976..4314)
                     /locus_tag="PL152_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1820 family protein"
                     /protein_id="HQR49282.1"
                     /translation="MPPSHIFKVVFHNQGKVYEIYARKVGHGSLFGFVEVEELIFGER
                     SAVVVDPSEERIQSEFAGVKRTYLPLHSVIRIDEVKKSGVSKVVAAEGSNVTQFPYPV
                     YTPGDPGPRR"
     gene            4364..>5202
                     /gene="tagH"
                     /locus_tag="PL152_08095"
     CDS             4364..>5202
                     /gene="tagH"
                     /locus_tag="PL152_08095"
                     /inference="COORDINATES: protein motif:HMM:TIGR03354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HQR49283.1"
                     /translation="MAIKLRVISDQYRELGEERSRVFGVNGGTIGRAPDNDWVLPDQK
                     RLVSGHHCDIEYRSGGYWLRDRSTNGVFVNESDEAVSETGPVPLQDGDRLRLGDYEIL
                     VSVDERMDFLPSESELESAEEHLDTGIGVKLDLDSLFTPRDTGDSSSISIRNAFGLKL
                     PKSFRKAAAREPQPVETSEPQLAPPIEDSPIEDTPGVEEPVAAAEPAPAEWALKTRAV
                     TRKELADALARRQSRIDARQQTLPFHQQATAWTDLRSAVQAFCRGAGIDATSLSAEAQ
                     SMLP"
BASE COUNT          830 a         1888 c         1744 g          740 t
ORIGIN      
        1 acccaggcgc gggcgttgcg gaacatccgc atccacccac tgtcctcgcc ccactccgcc
       61 gggtgccagg agttctggac cgtgcgatag acgcgctccg gatgcggcat gacgatcgtc
      121 gcccggccgt cgctgctcgt aagtcccgtg atgcccgacg gcgacccgtt cgggttggcc
      181 gggtacgcct cggccgggcg cccgtggttg tcgacgaatc gcaggctcac cagcccgctg
      241 cccacgcacg cgcgcatcgc ctcggcggtc gcgaactcgg ccctgccctc gccgtgcgcc
      301 acggcgatcg gcatgaccga ccccgccatg ccggcgaaga agacggacgg ggatggcagc
      361 acctcgacga gccccacgcg gccctcgaac tgctcggagc ggttgcgcac gaagcggggc
      421 cagtgggccg cgccgggtac gaggtctttc agcgctgcca tcatctggca accattgcac
      481 acgccgagcg tgaagctgtc ggtgcgcgcg aagaacgcct cgaactgggc gcgcagcatc
      541 gggttgaaca ggatcgattt cgcccagcct tcgcccgcgc cgagcacgtc gccgtacgag
      601 aagccgccgc aggccaccag cccgacgtag tcgtcgagcc gcgtgcgccc ggcgatcagg
      661 tcggtcatgt gcacgtccac cggcgcgaat cccgcgcgat ggaacgcggc cgccatctcg
      721 agctggctgt tcaccccctg ctcgcgcagg atcgcgaccg gcggtcgggt tcccttgacc
      781 aggaattggg ccgcggtatc tgcggctgga tcgaacgtca gccgggcgtg caggccgggg
      841 tccgcgggat ccgtggcacg cgcatactcc tcggcggcgc actccgggtt gtcgcgcagc
      901 gactgcatgc ggaacgtcgt ctcggaccac gcgcgccgca gctcggtgcg cgtcgcctgc
      961 agaatcgtgc ggccacggga ccggatcacg accgcgtcgt cggacgtgac ccgaccgatg
     1021 acgtggaccg agtccgcgag accagccctg ctgaattctg ccacgacttg cgcgaccgcc
     1081 gctgcctcga cctgcaggac cgcgcccagt tcttccgcga acatcgccga gacgaccgcc
     1141 gcgccgccct ctcccgcgat gagatccgcc tcgatgccgc accgaccggc gaatgccatc
     1201 tcgcacagcg tgacgaacag cccgccgtcg gaacggtcgt ggtaggccag gatccggccg
     1261 gcatcgcgca gcgacgtgat cgcgctgaaa aacgcccgca acgccgcagg gtcgtcgcag
     1321 tccggggcga ccgatccgac gcgcccgtac acctgcgcga cgcaggaacc ccccaggcgg
     1381 tccttgccga gccccaggtc cacgagcagc aggcgcgtgt cgcctcgatc ggtccgcagt
     1441 tccggcgtga gcgtccgtcg cacgtcttgc gcaggagcga atgccgacac gatgagggaa
     1501 acgggcgcca cgaccttgcg cgggccgtcg ctgccctgcc atgcggtctt catcgagagc
     1561 gagtccttgc ctaccgggat cgcgatcccg agtgccgggc agagttcctc gcccaccgca
     1621 cgcaccgcgt cgtacaggtc cgcgtcctcg ccgggctcgc cgcaagcggc catccagttc
     1681 gccgagagcc ggatgtcctc gatgcgggcg atgtccgcgg cggcgatgtt ggtcacggcc
     1741 tcggcgacgg ccatgcgcgc ggccgccgcc ggatccagca gggcgagcgg cgttcgctcc
     1801 cccatcgcca tcgcttcccc tcgggtcgag aagtagtcgc tcaccgtcac ggccacgtcg
     1861 ctcacgggga cctgccacgg tcccaccagc gggtcgcggc tgatcatgcc gcccaccgac
     1921 cgatcgccga tcgcgatcag gaatgtcttg tcggcgatcg tcggcagccg cagcagccgg
     1981 aatgccgcat cgcggacgtc gacatcccgg ccgtcgaaat cgtccgaggc gccgcgcaac
     2041 cggcggacgt cccgcgtcat gcgcggcggc ttgccgagca gcgtttccag ccgcatcccg
     2101 accggctcat tgccgaagag cggatcggcg acccggagcg tgccgtccga cgtcacctcg
     2161 ccgatcaccg cgtacgggca gcgttcgcgc gcgcagaacg ccgcgaaccg ctccatgtgc
     2221 ccggccgcga cgatcagcac gtagcgctcc tgcgcctcgt tgcaccagat ctcgagtggc
     2281 gacatgccgg gctcgtcgct cggcacggcg cgcaggtcga cgcggccgcc gcagccgccg
     2341 tgcgccacgg actcgggcac cgcgttcgac agcccgccgg cgccgacgtc atggatgagg
     2401 aggatcgggt tcgcgtcgcc gagcgcccag caggcgtcga tgacctcctg ggcccggcgc
     2461 tggatctcgg ggttgccgcg ttgcacggac gcgaagtcga ggtccgccga actcgcgccc
     2521 gcgcccaccg aggacgccgc gccgccgccg agcccgatca gcatcgcggg accgccgagc
     2581 accacgatct tcgcgccggc gacgatggtg gccttctcga cgtgctcgcg acggatgttg
     2641 ccgaggccac cggcgatcat gatcggcttg tggtaaccgc gcagcggcga gcgcccctcg
     2701 gcctcgacgc gctgctcgaa ggtgcggaaa tagccgcaga ttcccggccg gccgaactcg
     2761 ttgttgaacg aagcagcgcc gatcgggcct tcgagcatga tcgagagcgc cgacacgatg
     2821 cggtccggct tgcccaggtc ctgctcccac ggctgcaccg cgcccggaat gcgcaggttc
     2881 gacacgctga aaccgaccag ccccgccttc ggcttcgagc cgcggccggt cgcgccttcg
     2941 tcgcggatct cgccgccgga cccggtggcc gcgcccggga acggcgaaat cgccgtcggg
     3001 tggttgtgcg tctccacctt catcaggatg tcgatcggct cgtcggctcc cgcgtaggcc
     3061 ccgccgcccg gctgcgcgaa ccagcgccgg cccggccagc cctcgatgac ggcggcattg
     3121 tcgcgatagg ccgacagcac gccgcgcgga ttgcgcgcgt gcgtattgcg gatcatgccg
     3181 aacagggact tcgggcccct ggacccgtcg acgatccagt ccgcgttgaa gatcttgtgc
     3241 cggcaatgct cggaattcgc ctgcgcgaac atcatgagct cgacgtcggt cgggtcgcgc
     3301 ccgagcgacc ggaacgcatc cacgaggtaa tcgatctcgt cgtccgacag cgcgagcccg
     3361 agttcggcat tcgccgacac cagcgccgcg cgcccctgtg caaccagtgg cacggtcgag
     3421 agcggcttcg gcgccgcatg ctcgaagagc gcggccgccg ctgcggtgtc gcgcaacacc
     3481 gcctcggtca tgcggtcgtg gacgacggct gccgcggctt gccagtcctc gtcggcgagg
     3541 cgacgcgacg cgccgaggta ataggccacg ccgcgctcga tgcggcgcac ggcggccagg
     3601 ccgcacacgt gcgcgatgtc ggtggccttg ctggaccagg gggacacggt gccaatgcgc
     3661 ggcacgacga gcagcaactc accctggggc gcacccgccg acacggtggg tccgtaacgc
     3721 agcagcgact cgagcacccc ggcttcgcgc gcgtcgaggg ggcgctcgac gtccacgaag
     3781 tggacgtgac gggaatccag gcgctcgacc tctcgggtcc gctctcgtat cgacgacagg
     3841 agcttggcga gacggaaatc cgagactgcg ggggcgccgc tgagtgtgag cattcttcga
     3901 agccgccgcc gggctggcag gggcgcggaa tgataccgga gcgccggagc ccgaaccagc
     3961 gcgagcggcc gccggctagc gacgcggccc cggatcgccg ggcgtgtaga cgggatacgg
     4021 gaattgcgtg acgttcgagc cttcggccgc gaccaccttg ctcacgccgc tcttcttgac
     4081 ctcgtcgatg cggatcaccg agtgcagcgg caggtaggtg cgcttcacgc ccgcgaactc
     4141 cgactggatc cgttcctcgg atggatccac caccaccgcg gaacgctccc cgaagatcag
     4201 ttcctcgacc tccacgaagc cgaacagcga gccgtgcccc accttgcggg catagatctc
     4261 gtaaaccttg ccctggttgt ggaaaacgac tttaaaaatg tggctgggag gcatgaggtg
     4321 cttgcgattt caggccggcc catattacaa ggggagcctg accatggcca tcaagctacg
     4381 cgtcatcagc gaccagtatc gcgaactcgg cgaggagcgc tcgcgcgtgt tcggcgtcaa
     4441 tggcgggacc atcggccggg cccccgacaa cgactgggtc ctgcccgacc agaagcgact
     4501 ggtgtccggg catcactgcg acatcgaata ccgcagtggc ggctactggc tccgtgatcg
     4561 cagcacgaac ggggtgttcg tcaatgaatc cgacgaggcg gtctcggaga ccggcccggt
     4621 ccccttgcag gacggagacc gcctccgcct cggtgactac gagatcctgg tgagcgtcga
     4681 cgagcgcatg gacttcctgc cctccgaatc cgagctggaa tcggccgagg agcacctcga
     4741 caccggaatc ggcgtcaagc tcgacctcga cagcctgttc acgccgcgcg acacgggcga
     4801 ttcgagttcg atctcgatcc gcaatgcatt cggcctgaag ctgcccaagt cgttccgcaa
     4861 ggctgcggcg cgcgagccgc agcctgtcga aacgtccgaa ccgcagctgg cgccgccgat
     4921 cgaagactcg ccgatcgaag acaccccggg cgtcgaggaa ccggtcgcgg cggcagagcc
     4981 cgcgcccgcc gagtgggcgc tcaagacccg cgccgtcacg cgcaaggaac tcgccgacgc
     5041 cctcgcacgc cgccagagtc gcatcgacgc ccggcagcag acactcccgt tccaccagca
     5101 ggcgacggcc tggacggacc tgcgctcggc cgtgcaggcg ttctgtcgcg gcgcaggcat
     5161 cgacgccacg tcactcagcg cggaagcgca gtcgatgctg cc
//
LOCUS       DAOMFD010000138         5176 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6575_length_5176_cov_31.257762, whole genome shotgun sequence.
ACCESSION   DAOMFD010000138 DAOMFD010000000
VERSION     DAOMFD010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6575_length_5176_cov_31.257762"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..309
                     /locus_tag="PL152_08100"
     CDS             <1..309
                     /locus_tag="PL152_08100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49284.1"
                     /translation="RVGMSGAEVATVLGSPNVVTTDEERREVWIYDKISTTSAYSTSS
                     GGIWALILGGGSSVGGAGGGSAGQSAGAAATTQKTLTVIIKFDADKKVRDFAYHTSSF
                     "
     gene            314..883
                     /locus_tag="PL152_08105"
     CDS             314..883
                     /locus_tag="PL152_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49285.1"
                     /translation="MTGARARPGGSGVAALSLVCMLVAAGGCATIPPDALVLSPESLE
                     LRQMQTRRIDGISEKDLLYASVGVLQDLGFEIDEAETKLGLIVASKDRSAFTPSQIAG
                     AIALALLGVQSDIDKTQKIRVSLVTHPALDSDGKPRPDSQVIRITIQRIVWNTQNQVT
                     RIEAIEEPEIYQKFYEKLSKSIFLEVHAT"
     gene            880..1302
                     /locus_tag="PL152_08110"
     CDS             880..1302
                     /locus_tag="PL152_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49286.1"
                     /translation="MNRSNAAVFVACCLALVAGCAPSTEAVLETGQSAVALRSFQQRA
                     FDTTDKEKTMRSVIATLQDLSFVVDKADLELGTVSATKLSGYQLRITVTVRPRGDTQM
                     LVRANAQYMDKAVEDPKPYQDFFTALEKSMFLTAHQVD"
     gene            complement(1319..1894)
                     /locus_tag="PL152_08115"
     CDS             complement(1319..1894)
                     /locus_tag="PL152_08115"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR49287.1"
                     /translation="MSRKYTLRKRADRLADTRRRIVEATVALHGEVGPASTTLSMIAE
                     HAGVQRHTLYAHFPDERSLFLACSGLAMERDPLPDAAPWAGIPTARDRLRQGLSALYG
                     WYARNESLAACVLRDAEHHALTREITELRFGRPMAAYRQVLGAGLAPNQQALLAVALD
                     FHTWRTLAREAQMSPEAAAGVVAEAVASAPS"
     gene            2004..2777
                     /locus_tag="PL152_08120"
     CDS             2004..2777
                     /locus_tag="PL152_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49288.1"
                     /translation="MSMDTTTVSEIAEGLYRISTYVPAIAPPAGFTFNQFLVVGDEPL
                     LFHTGPRRMFPLVRDAVAQVVAPERLRWITFGHVEADECGSMNEWLAIAPRAQVAHGA
                     IGCSVQLMDLADRPPQELANDQVLDLGRGKRVRYIDTPHTPHGWDAGVIFEESTSTLL
                     CGDLFTQVGRGSPLTASDIVGPAIAAEDMFKYSALNPDMGATIRRLAALAPRTLALMH
                     GPSFNGDTVGALRALADDYDRRGWRAQAAAPAAREQRIA"
     gene            2825..3625
                     /locus_tag="PL152_08125"
     CDS             2825..3625
                     /locus_tag="PL152_08125"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR49289.1"
                     /translation="MFEAIEQEIDTPALRIRYHLAPWDAATVGAPVAAISSLEVRDAA
                     AATAEFPAFREWVRRSGVVLVSCRLPHGRLVECGFLESQGFRFIELNYRPELGNLEQI
                     KLDDSAQYAVEPASGADEDAIADMARQVFDASRFHNDPMIDARIGHLRYQAWVHNAFR
                     NPRQSVLKCHVDGNIVAFFVVEAPQPQGRFWSLVGLAPGMTGRGIGAAVWRAVLRWHR
                     DEGVTHVATSISSLNLRVLNLYVKLGFRFPEPEITLHSCPLGPIRSPE"
     assembly_gap    3807..3906
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3907..4698
                     /gene="rffA"
                     /locus_tag="PL152_08130"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             <3907..4698
                     /gene="rffA"
                     /locus_tag="PL152_08130"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: protein motif:HMM:NF008687.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019180 -
                     dTDP-4-amino-4,6-dideoxygalactose transaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009246 - enterobacterial common antigen
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HQR49290.1"
                     /translation="PRTRAVWPVHYAGVACEMDEIMAIARRHGLTVVEDAAQGVFASY
                     RGRWLGTIGDLGCYSFHETKNFTCGEGGALLVNAERFLHRAEILREKGTNRSQFLRGQ
                     VDKYTWVDVGSSYVPSDLLAAFLYAQLEHMEAITAKRRAVFERYMRELSPLAERGLVR
                     LPVVPAQCSTNYHMFYLLTAGIEERTALIEHLKRAGIQSVFHYVPLHTSPVGRSLGGR
                     EGMLPVTEDASARLLRLPLYCDMTATEVGEVIVEIYRFFGLSAPT"
     gene            4731..>5176
                     /locus_tag="PL152_08135"
     CDS             4731..>5176
                     /locus_tag="PL152_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49291.1"
                     /translation="MAKVLHVMMKLPDKVGSDFQQFAHQSLGQHTHSFVAFRSGKVEA
                     THPSSRTYELLPRGVLPKFLALRRCLEASRPDVVIFHGMNYELRETLALLALYATTDI
                     RYRAVWYIWGADVYRFQNRFRGFVPAVSEFFRRRLLRRVFYAATLVP"
BASE COUNT          856 a         1633 c         1705 g          882 t
ORIGIN      
        1 cgggtcggca tgagtggcgc ggaggtggcg acggtgcttg gctcgcccaa cgtcgtgacc
       61 acggacgagg agcgccgcga ggtctggatc tacgacaaga tttcgacgac gtctgcctat
      121 tcgacgagca gcggcgggat ctgggcgctg atcctcgggg gcggctccag cgtcggcggg
      181 gcgggcggcg gcagcgccgg gcagtcggcg ggagcggcgg cgacgacgca gaagacgctg
      241 accgtcatca tcaagttcga cgccgacaag aaggttcgcg acttcgccta ccacacgtcg
      301 agcttctgat gccatgacgg gcgcgagggc gcgaccgggc ggatcgggcg tagcggcgct
      361 ctcgctggtg tgcatgctcg tggcggcggg cggctgcgcg acgatcccgc cggacgccct
      421 ggtgctgagt cccgaatcgc tcgaactgcg ccagatgcag acgcgacgca tcgacggcat
      481 cagcgagaag gacctgttgt acgcctccgt cggcgtgctc caggacctcg gattcgagat
      541 cgacgaggcc gagacgaagc tcggcctgat cgtcgcgtcc aaggaccggt ccgccttcac
      601 gccctcccag attgccggcg ccattgcgct cgcgctgctc ggcgtccagt ccgacatcga
      661 caagacgcag aagatccgcg tttcgctcgt gacgcacccg gcgctcgatt cggatggcaa
      721 gccccgtccc gacagccagg tgatccgcat cacgatccag cgcatcgtgt ggaacacgca
      781 gaatcaggtg acccgcatcg aggcgatcga ggagccggag atctaccaga agttctacga
      841 gaagctctcg aagagcatct tcctcgaggt gcacgcgaca tgaatcgatc gaatgcagcc
      901 gtgttcgtgg cctgctgcct ggcgctcgtc gctggctgcg ccccttcgac cgaggcagtg
      961 ctcgagaccg ggcagagtgc cgtggcgctg cgcagcttcc agcagcgtgc cttcgacacg
     1021 acggacaagg aaaagaccat gcgcagcgtg atcgcgacgc tgcaggatct cagcttcgtc
     1081 gtggacaagg ccgacctgga actcggcacc gtcagcgcaa ccaagctgtc cggctaccag
     1141 ctgcggatta ccgtcaccgt tcggccgcgc ggcgacacgc agatgctggt gcgtgccaat
     1201 gcgcaataca tggacaaggc tgtcgaggat ccgaagccgt atcaggactt cttcaccgcg
     1261 ctcgagaagt ccatgttcct gaccgcgcac caggtggact gacgcctcgg ttgccgcgtc
     1321 aactcggcgc cgatgcgacc gcctcggcga ccacgcccgc agcggcctcg ggtgacatct
     1381 gcgcctcgcg cgccagcgtc cgccaggtgt gaaagtcgag ggcgaccgcg agcagtgcct
     1441 gctggttcgg ggccaggccc gcacctaaaa cctgccgata ggccgccatc ggccgaccga
     1501 atctcagctc ggtgatctcg cgggtaagcg cgtgatgctc cgcatcgcgc agcacgcatg
     1561 cggccaggct ctcgttccgg gcgtaccagc catacagtgc cgacagcccc tgacgcagcc
     1621 ggtcgcgagc agtcgggatc ccggcccagg gagcggcatc cggcaaaggg tcccgctcca
     1681 tggccaggcc ggagcacgcg agaaacaggc tccgctcgtc gggaaagtgg gcgtagagag
     1741 tgtgccgctg aacgccggcg tgctccgcga tcatgctcag cgtcgtcgac gcgggcccga
     1801 cttcgccgtg cagggcgacc gtcgcctcga cgatccgccg gcgcgtatcc gccagtcgat
     1861 cggcacgctt cctgagggtg tatttcctgc tcattcgcat taactgcaca tatgtgtgca
     1921 gtcatagttg acaccaggct attttagatg cacagtattg tgcactcaag tcttgtcgat
     1981 cgtccgtaac actcaagggg ggaatgagca tggatacgac caccgtcagc gaaattgccg
     2041 aaggccttta tcgaatctcg acctacgtgc cggccatcgc gccgcccgcc ggcttcacct
     2101 tcaaccagtt cctcgtcgtc ggcgacgagc cgttgctgtt ccacacgggt ccgcgccgga
     2161 tgtttccgct ggtccgcgac gccgtggcgc aggtcgttgc gcccgagcgg ctgcgctgga
     2221 tcacgttcgg gcacgtcgag gcggacgagt gtggctcgat gaacgagtgg ctcgcgatcg
     2281 cgcctcgcgc gcaggtcgca cacggtgcga tcgggtgttc ggtgcagctg atggatctcg
     2341 ccgaccggcc tccgcaggaa ctcgcgaacg accaggtgct cgacctcggc cggggcaagc
     2401 gggtccgcta catcgacacg ccgcacaccc cgcacggctg ggacgcgggc gtgatcttcg
     2461 aggagtcgac atcgacgctg ctgtgcgggg acctgttcac gcaggtcggc cgcgggtcgc
     2521 cgctgacggc gagcgacatc gttggcccgg caattgccgc cgaggacatg ttcaagtact
     2581 ccgcgctgaa tcccgacatg ggcgcgacca ttcgacggct cgccgcactg gcgccgcgca
     2641 cgctcgcgct gatgcacgga ccgtcgttca acggcgacac ggtgggtgcg ctgcgcgccc
     2701 tggcggacga ctacgaccgg cgtggctggc gagcgcaggc ggctgcaccc gcagccaggg
     2761 agcagcggat cgcctagact tggcggtacc gcggcagggc tggctgcgtt tcggatggtc
     2821 gttgatgttt gaggcgatcg aacaggagat cgacacccct gccctgagga tccgctacca
     2881 cctggcgccg tgggatgccg cgaccgtcgg agcgccggtg gcggcgatct cgtcgctcga
     2941 agtgcgggac gcggcggccg cgacggcgga gttcccggcg ttccgggagt gggttcgccg
     3001 gagcggagtg gtcctggtca gctgcaggct cccgcacggg cgactggtcg aatgcggctt
     3061 cctggagtcg cagggttttc gattcatcga actcaactac cgtccggagc ttggcaacct
     3121 cgagcagatc aagctcgatg actcggcgca gtacgcggtg gagccggcgt cgggggcgga
     3181 cgaggatgcg atcgcagaca tggcgcgcca ggtcttcgac gccagccgct ttcacaatga
     3241 tccgatgatc gatgcgcgaa tcggacatct gcggtaccag gcgtgggtac acaacgcatt
     3301 ccggaatccg cggcagtcgg tcctgaagtg ccacgtcgat ggaaacatcg tcgcattctt
     3361 cgtcgtcgaa gccccgcagc cccaaggccg cttctggtcg ctcgtcggcc tggccccggg
     3421 catgaccggt cgtggcatcg gcgccgcggt ctggcgcgcc gtcctgcgat ggcatcggga
     3481 cgaaggcgtc acgcacgtag cgaccagcat ttcctcgctc aatctccggg tcctgaacct
     3541 ctacgtgaaa ctcggctttc ggtttccgga gccggaaatt acgctgcact cctgtccact
     3601 cggaccgatc aggagcccgg agtgaaccgc cggacagcga tccgcctcct tgcagcgccg
     3661 agcaggggtc agtagtgggt tatcgaatcc cgttcaacaa gccgccggtt gctggatcgg
     3721 aggtcgaata catccgcgaa gcaatcgcgg ccggccggac gagcggcgat gggccgttca
     3781 cgaggcgctg tcagcagctc ctggagnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnccgc ggacacgagc ggtgtggccc gtgcattacg ccggtgtcgc ctgcgagatg
     3961 gacgagatca tggccatcgc acgcaggcac ggcctgacgg tggtcgagga cgcggcacag
     4021 ggggtattcg cgtcctaccg tgggcgctgg ctcggcacga tcggcgatct cggctgctac
     4081 agctttcacg agacgaagaa tttcacgtgc ggcgaaggcg gggcgctgct ggtgaatgca
     4141 gagcgattcc tccatcgggc cgagatcctg cgcgagaagg gcaccaaccg cagccagttc
     4201 ctgcgcggac aggtcgacaa gtacacgtgg gtggacgttg gatcgtcgta tgttccgtcc
     4261 gatctgctcg cggcatttct ctacgctcag ctcgagcaca tggaggcgat cacggcgaaa
     4321 cgccgggccg tcttcgagcg ttacatgcgc gaactctcgc cgctggccga gcgtggactg
     4381 gtgcggctgc ccgtggtgcc cgcacagtgc agcaccaact accacatgtt ctacctgctg
     4441 acggcgggca tcgaggagcg cacggcgctg atcgagcacc tgaagcgtgc cggcatccag
     4501 agcgtgtttc attacgttcc cctgcacact tcacccgtcg gccggtcgct gggtggacgc
     4561 gagggaatgc ttccggtgac cgaagatgcg agtgcgcgtc tcctgcggtt gcccctgtac
     4621 tgcgacatga ccgcgaccga ggttggcgag gtaatcgttg agatctatcg gttcttcggg
     4681 ctttcggccc cgacatgacg tgggcgccta gccggatccg gacccgctcc atggcgaaag
     4741 tactgcacgt gatgatgaag ttgcccgaca aggtcggcag cgatttccag cagttcgcgc
     4801 accagtcgct gggccagcac acgcactcgt tcgtcgcgtt caggagcgga aaggtcgaag
     4861 cgacccaccc gagttcccgc acctacgaac tcctgcctcg gggcgtgttg cccaagttcc
     4921 tggcgctgcg caggtgtctc gaggcgtcgc ggccggacgt cgtgatcttt cacggcatga
     4981 actacgagtt gcgcgagacg ctggctctcc tggcgctcta tgccacgacc gacattcgct
     5041 acagggcggt ttggtacatc tggggcgcgg atgtctatcg gttccagaat cgcttccgtg
     5101 gtttcgtgcc ggcggtgtcc gagttctttc gccggcgact gctgcggcga gttttctatg
     5161 ccgccacgct cgtgcc
//
LOCUS       DAOMFD010000139         5172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6584_length_5172_cov_29.932969, whole genome shotgun sequence.
ACCESSION   DAOMFD010000139 DAOMFD010000000
VERSION     DAOMFD010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6584_length_5172_cov_29.932969"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /gene="aroC"
                     /locus_tag="PL152_08140"
     CDS             complement(<1..472)
                     /gene="aroC"
                     /locus_tag="PL152_08140"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQR49292.1"
                     /translation="MSGNTFGQLFTVTTFGESHGPALGCIVDGCPPGLVLAEEDIQRD
                     VERRRSGTSRYTSQRREPDVVRILSGVFEGRTTGTPIGLLVQNEDQRSYDYEKIKDRF
                     RPGHADYTYQHKYGFRDYRGGGRSSARETVMRVAAGAIARKYLAEHAGVVVRGYL"
     gene            complement(494..1423)
                     /gene="prmB"
                     /locus_tag="PL152_08145"
     CDS             complement(494..1423)
                     /gene="prmB"
                     /locus_tag="PL152_08145"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HQR49293.1"
                     /translation="MKATPPFHSPPPEAPTVADLVRYGAQEFDQAGLDYGHGMDNPLD
                     ESAALVFHALALDHAHAAEAYGQRVQPGDAERVLELFRERIVRRVPAAYLMGRMWFAG
                     LEFEVDPGVIVPRSPFAELIAARFEPWIDPAAVHGILDIGTGSGCIAIACAKAFPGAT
                     VDAVDVSPAALAVARRNVERHGVGDRLNLLEGDVFTPVGGRRYDLIVSNPPYVSDAEM
                     AALPAEYRHEPDLALRAGSDGLEIVRRILAGAAQHLAPGGALFVEVGDSDERLSQAFP
                     RLPFTWLEFEHGGGGVFMLRRNELQAPPTRGVA"
     gene            1505..2125
                     /locus_tag="PL152_08150"
     CDS             1505..2125
                     /locus_tag="PL152_08150"
                     /inference="COORDINATES: protein motif:HMM:NF014667.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HQR49294.1"
                     /translation="MRIAILAIVAFAGGLLIARAVFPPRPAVPPTERATVLSEAKPLP
                     ALDLLDQDGRRLGTDFFKGHWTIVFFGYTSCPDVCPTTLATLAQALKGMADMPLAERP
                     RVLLVTVDPERDDPARLAAYVRFYDPSFLGATGTPAAVAAAAAAFGVPYAKIALPEGG
                     YTMDHGSGLTIVGPSGAVVAYSSAPHEAPAIVRDYRKVVAYAEGLR"
     gene            2122..>2615
                     /locus_tag="PL152_08155"
     CDS             2122..>2615
                     /locus_tag="PL152_08155"
                     /inference="COORDINATES: protein motif:HMM:NF014696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HQR49295.1"
                     /translation="MSYEEAGASDRAFALLQDLLPQHLLSRTMHRLARSKRPFLRNAL
                     VRSVLRAYPQIQMREAAQPDPLAYESFNAFFTRALRVDARVVAGGAGDVASPVDGTVS
                     QLGTIHSGQILQAKGVHYSPAALLADETATSRYEGGAFACLYLAPYNYHRIHMPLAGR
                     LTATR"
     assembly_gap    2616..2715
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2716..3152
                     /gene="asd"
                     /locus_tag="PL152_08160"
     CDS             <2716..3152
                     /gene="asd"
                     /locus_tag="PL152_08160"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013344026.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="archaetidylserine decarboxylase"
                     /protein_id="HQR49296.1"
                     /translation="YNYHRIHMPLAGRLTATRYVPGALYSVNAATARAIPGLFARNER
                     VVCEFDTALGPMAMVLVGALFVGSIETVYAGEINPPRARGGSITNIAAGAGTEFAKGA
                     ELGRFNMGSTVVLLLGNGTVRHADGLGPGTPVRMGQVLARAG"
     gene            3149..4123
                     /gene="epmA"
                     /locus_tag="PL152_08165"
     CDS             3149..4123
                     /gene="epmA"
                     /locus_tag="PL152_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HQR49297.1"
                     /translation="MTASLPRDWMPTASLETLRLRARMLERVRAHFAATGALEVETPV
                     LVLAPVTDVHLESLAVHRADGTRAGFLHTSPEYAMKRLLSAGAPDIYQVCRVFREGER
                     GRRHNPEFTMVEWYRLGIDHHALMDDVERLLRALLEPARSVGATRRITYGGAFQEALA
                     LDPLAASPTQIAATLTARGLDVPASIRDDRDALLDLGMSLLVAPGFPADRVTFVHDFP
                     ATQAALARIRGPVASRFEAFWGGLELCNGFHELGDAAEQSRRFEADRAERRRRGQPDR
                     DPDHRFLAGLAAGLPPCAGVALGFDRVVMIATGASSIDEVVAFPAERA"
     gene            4163..4672
                     /locus_tag="PL152_08170"
     CDS             4163..4672
                     /locus_tag="PL152_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070191 - methionine-R-sulfoxide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HQR49298.1"
                     /translation="MHDTTVIALDDKPRAYAELEQSLRALLSGENDFIAGAANTAALV
                     YWSLPGLNWAGFYFVEPRSGDLVLGPFQGKPACVRIAIGKGVCGTAAARRATLVVPDV
                     HAFPGHIACDAASNSEVVVPVVDGDRLIGVLDLDSPVHGRFDDDDARGLEALVRVFVA
                     ATRGHAGMP"
     gene            complement(4676..5020)
                     /locus_tag="PL152_08175"
     CDS             complement(4676..5020)
                     /locus_tag="PL152_08175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49299.1"
                     /translation="MLKNVKDPKDLKDILLSVLQTSGTMAGLSMALVGIINLRAANTS
                     TETVADDLFLLSSVGFLVVCYLVFFALRRLKTPNIRTWTALIDVLFLLSMTLLVLSGF
                     VVLYAFGVGYGH"
BASE COUNT          731 a         1816 c         1752 g          773 t
ORIGIN      
        1 cgagatagcc gcgaacgacg acgccggcgt gctcggcgag gtacttgcgc gcgatggcgc
       61 ccgccgccac gcgcatgacc gtctcgcgcg ccgacgagcg gccgccgccg cgatagtcgc
      121 ggaacccgta cttgtgctgg taggtgtagt cggcgtggcc cggccggaac cggtccttga
      181 tcttttcgta atcgtaggac cgctggtcct cgttctgcac cagcaggccg atgggcgtcc
      241 ccgtcgtgcg gccctcgaag acgcccgaga ggatgcgtac cacgtcgggt tcgcggcgct
      301 ggctcgtgta gcgcgacgtc ccggagcggc ggcgctcgac gtcgcgctgg atgtcctctt
      361 ccgcgagcac gaggccgggc gggcacccgt ccacgatgca acccagcgcg ggcccgtggc
      421 tctcgccgaa cgtcgtgacc gtgaagagct ggccgaaggt attgcccgac atctggtgtt
      481 ccccggacga ctgctaggcc acgccgcgcg tgggcggggc ctgcaactcg ttccttctta
      541 gcatgaacac gcctcccccg ccgtgctcga attcgagcca ggtgaacggc aggcggggga
      601 aagcctgcga caggcgctcg tcgctgtcgc cgacttccac gaacagggca ccgccgggtg
      661 cgaggtgctg cgccgctccg gcgagtatgc ggcgcacgat ctccaggccg tcgctgcccg
      721 cgcgcagcgc gaggtccggt tcgtggcggt actcggccgg cagtgccgcc atctccgcat
      781 cgctcacgta gggcgggttc gacacgatca ggtcgtaacg acgcccgccg accggcgtga
      841 acacgtcgcc ttcgaggagg ttcagtcggt cgccgacgcc atggcgctcc acgttgcgac
      901 gggccaccgc gagcgcggcc ggcgacacgt ccaccgcgtc caccgtcgcg ccggggaacg
      961 ccttcgcgca ggcgatcgcg atgcagcccg atcccgttcc gatgtccaga atgccgtgga
     1021 ccgccgctgg atcgatccag ggctcgaagc gcgcggcgat caactcggcg aatggcgagc
     1081 gcggcacgat cacgcccgga tcgacctcga actcgagacc ggcgaaccac atgcgcccca
     1141 tcaggtaggc cgccgggacg cgccggacga tgcgctcgcg gaacagttca agcacgcgct
     1201 cggcgtcacc aggctggacg cgctgcccgt acgcctccgc cgcgtgggcg tgatcgaggg
     1261 cgagcgcgtg gaagaccagc gccgccgatt cgtccagcgg attgtccatg ccgtgtccgt
     1321 agtcgagtcc ggcctgatcg aactcctgcg caccgtaacg gaccaggtcg gcgacagtcg
     1381 gcgcctcggg tggcggcgag tggaacggcg gtgtcgcctt catgtcacgt gcgcccgttg
     1441 tgggataatc gcggccccgt ccggccggac gcccaccacg cacccagaca tgtccgctcg
     1501 cttcatgagg atcgccatcc tcgcgattgt agcgtttgcc ggcgggctgc tgatcgcccg
     1561 cgcggtgttc ccgccgcggc cggccgtgcc gccgaccgag cgcgcgaccg tgctgtccga
     1621 ggcgaagccg ctgcctgccc tggatctcct cgaccaggac ggacggcgac tcggcaccga
     1681 ctttttcaag ggccactgga cgatcgtgtt cttcggctac acgagctgcc cggacgtctg
     1741 tccgaccacg cttgcgacgc tggcacaggc gctcaagggg atggcggaca tgccgctggc
     1801 cgagcggccg cgcgtgctgc tcgtcaccgt cgatccggag cgcgacgatc ccgcgcggct
     1861 cgccgcctac gtgcggttct acgacccctc gttcctgggc gcgaccggca cgcccgccgc
     1921 cgttgcggcg gcggcagcgg ccttcggcgt tccatacgcg aagatcgcct tgcccgaagg
     1981 cggctatacg atggaccacg gttccgggct cacgatcgtc ggcccgtcgg gcgccgtcgt
     2041 ggcgtattca tcggctccgc acgaagcgcc ggcgatcgtc cgcgactacc ggaaggtcgt
     2101 cgcctacgcc gagggcctgc ggtgagttac gaggaagcag gcgcatcgga ccgggccttt
     2161 gcgctgttgc aggacctgct gccgcagcac ctgctgtcgc gcacgatgca ccggctcgcg
     2221 cgcagcaagc gtccgttcct gcgcaatgcg ctggtccgct ccgtactgcg cgcgtacccg
     2281 cagatccaga tgcgcgaggc cgcgcagccg gacccccttg cctacgagtc gttcaatgcg
     2341 ttcttcacac gcgcgctgcg cgtggacgca cgcgtcgtgg cgggcggcgc cggtgacgtc
     2401 gcctcgccgg tggacggcac ggtgagccag ctcggcacga ttcactccgg ccagatcctc
     2461 caggcgaagg gcgtccacta ctcgccggct gccctgctcg ccgacgaaac tgccacgtcc
     2521 cgctacgaag gcggcgcttt cgcgtgcctc tacctcgcgc cgtacaacta tcaccgcatc
     2581 cacatgccac tcgccggccg gctcacggcg acgcgnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2701 nnnnnnnnnn nnnnncgtac aactatcacc gcatccacat gccactcgcc ggccggctca
     2761 cggcgacgcg ttacgtgccc ggtgcgctct atagcgtcaa tgccgcgacc gcgcgggcga
     2821 tcccgggcct cttcgcccgc aatgagcgcg tggtgtgcga gttcgacacg gccctggggc
     2881 ccatggcgat ggtgctggtg ggcgcgctgt tcgtcggcag catcgagacg gtgtacgccg
     2941 gcgagatcaa tccaccccgc gcccgcggcg ggtcgatcac gaacatcgcc gcgggagccg
     3001 gcacggaatt cgcgaagggc gcggagctcg gccgcttcaa catgggctcc accgtggtcc
     3061 tgctgctggg caacgggacc gtccgccacg ccgacggcct gggccccggc acgccggtgc
     3121 gcatgggcca ggtgctcgcc cgcgccggat gaccgcgtcg ttgccccgcg actggatgcc
     3181 gacggcgtcg ctcgagacgc tgcgcctgcg ggcgcggatg ctcgagcgcg tgcgggcgca
     3241 cttcgccgcg accggcgcgc tcgaggtgga aacgccggtg ctggtgctgg ccccggtcac
     3301 ggacgtgcac ctcgagtccc ttgcggtgca ccgcgccgat ggcacgcggg cgggcttcct
     3361 gcacacgtca cccgagtacg ccatgaagcg cctgctcagc gccggcgcgc ccgacatcta
     3421 ccaggtgtgc cgcgtgtttc gcgaaggcga gcgcggtcga cgccacaatc ccgagttcac
     3481 gatggtcgag tggtaccgcc tcggcatcga ccaccatgcg ctcatggacg acgtcgaacg
     3541 cctgctgcgt gcgctgctgg aaccggcgcg cagcgtgggc gcgacgcggc ggatcaccta
     3601 cggcggagca ttccaggagg cgctggcact ggacccgctg gccgcatcgc ccacgcaaat
     3661 cgcggcaacg ctcacagctc gcgggctgga cgtgccggcg tcgatacggg acgatcgtga
     3721 tgcgctgctc gacctgggaa tgtccctgct cgtggcaccc ggattcccgg cggaccgcgt
     3781 cacgttcgtc cacgacttcc cggccacgca ggcggcgctc gcacgcatcc gcggcccggt
     3841 ggcctcgcga ttcgaggcat tctggggcgg gctcgagctc tgcaatggat ttcacgaact
     3901 cggtgacgcc gccgagcagt cgcgccgctt cgaagccgat cgcgcggagc gccggcggcg
     3961 cggccagccc gatcgcgacc cggaccaccg gttcctcgcg gggctcgcgg ccgggttgcc
     4021 gccgtgcgcc ggcgtcgcgc tcggtttcga ccgggtcgtg atgatcgcga cgggcgcatc
     4081 cagcatcgac gaggtcgtcg cgttcccggc ggagcgcgcc tgatgcgccg cgcccccacc
     4141 gcaggcgacg cgagtaacca ccatgcatga caccaccgtg attgcgctcg acgacaagcc
     4201 ccgcgcctac gcggagctcg agcagtcgct gcgcgcgctg ctctccggcg agaacgattt
     4261 catcgccggc gcagccaaca ccgcggcact cgtttactgg agccttccgg gcctcaactg
     4321 ggccggcttc tatttcgtgg aaccgcgctc cggcgacctg gtgctcgggc cgttccaggg
     4381 caagcccgcg tgcgtgcgca tcgccatcgg caagggcgtg tgcggcaccg cggccgcgcg
     4441 acgcgcgacg ctcgtggtcc ccgacgtgca cgcgtttccg ggacacatcg cctgcgatgc
     4501 ggcctccaat tccgaagtcg tggtgccggt cgtcgacggc gaccggttga tcggcgtgct
     4561 ggatctcgac agcccggtgc acgggcgatt cgacgacgac gacgcgcgcg ggctcgaggc
     4621 gctggttcgc gtgttcgtcg cggccacgcg gggccacgcc gggatgccct gacgctcagt
     4681 ggccgtagcc gactccgaag gcgtacagga cgacgaagcc ggacagcacc agcagcgtca
     4741 tcgacagcag gaacagcacg tcgatcaggg ccgtccacgt gcggatgttc ggcgtcttca
     4801 gtcgtcgcag cgcgaagaag accaggtagc agacgaccag gaatccgacc gacgagagga
     4861 ggaacaagtc gtcggcgacg gtctcggtgc tggtgttggc cgcgcggagg ttgatgatgc
     4921 cgacgagcgc catgctgaga cccgccatgg tgccgctcgt ctgcagcacc gacagcagta
     4981 tgtccttgag gtccttggga tccttgacgt tcttcagcat gcccgcaccc gcgggcgcat
     5041 cagcccggac ccgtgggact cgccgtttgg accgccgaag gacggaccgg ttccgagtct
     5101 cggcgcgctg cttgcgccgc cggatcagcc cttggcgcgg gacaggtacg cgccggagcg
     5161 cgtatcgacg cg
//
LOCUS       DAOMFD010000140         5129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6673_length_5129_cov_35.192747, whole genome shotgun sequence.
ACCESSION   DAOMFD010000140 DAOMFD010000000
VERSION     DAOMFD010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5129
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6673_length_5129_cov_35.192747"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            51..2375
                     /locus_tag="PL152_08180"
     CDS             51..2375
                     /locus_tag="PL152_08180"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HQR49300.1"
                     /translation="MSILSRLLGNSPPATEVSSTSAPAAPVEAPRPDPAVRAREEEAS
                     VSQAIAAGDMTAVGTWVLDGSTTRIRQMAARAVTDLEQLRELIRATRGGNDKNVHRIL
                     ADKRDELLAEMRHAQQLQADLETTAADIAQHSERPYDALYSPTLERLEAQWRALEPQS
                     AETLRDSVARQLERARAVVDEHRHAIEVEAERKRSAALAAEELRRQRELEARAAAEAA
                     AEAAAEQARARETERQAEITRQEAEAAEVRHLLGLLRQAQAALDQGGTARAARFRDAI
                     EERLPSAPALPPWFARKLQEVASRIEELKDWKTFRVVPKRAELVERMQSLVGADMSPE
                     ELARQIRRLRDEWRTLNRGAAEDPSPEWQAFDDAATRAYEPCREHFAQQAERRRENQA
                     HREAMLERLSAFAAQQAGEDVNWRAVQQAIFESRREWQQYAPVDQAAARPLQERFRAV
                     LQELQGRLDAEYARNVQAKRELIARTAALADVKDTRQAIEEAKSLQRKWKTVGLVPRQ
                     QDNQLWEEFRRHCDAVFERSAQEWAAHGASLDANQARAIALCEELEGLAGSADEAPPS
                     DRRPLGEVRAEFDSLELPRASARELRRRFADAVNRYGEAARRHRAEAERRAWSDLFGV
                     GSRLNEYALAVAQGRSPEDCAAFRESAASAVAGLAHAPKGTRATLEQQMARIAAGEIG
                     TDLAANESARRMLCVRAELIAGVPTPPEDLELRREYQMQRLVAAMGRGERPAPADLDA
                     LALEWIAAGPVEAVVHDALLARLERCRGTLGGAA"
     gene            2480..3889
                     /locus_tag="PL152_08185"
     CDS             2480..3889
                     /locus_tag="PL152_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HQR49301.1"
                     /translation="MPRTAKKAATRARSTARRKPAARAKPSIRSKRPARKRDGRVIAL
                     FPEAAFGPALNSVGIGQALEKLGHKVVFLSDPGFLSVYEGYGFEAHPVNLSEPLPPEE
                     MAKFWVDFINGHIPNFRKTPLEQVDNYVKDCWGMIVQTAKWAEKDLPGVLEKVRPDVI
                     CVDNVILFPAIKRYARKHRIPWVRIISCSENEIEDPDIPPHLSGAGATDRKAQAAYRR
                     HFRQVIKPIHEEFNAFLKSVGERRYPLGQFFEASPHMNLLLYPSAVKFKRRHRLPARQ
                     FQYLEGCVRREKPYQVPRFKANDDQPLLYVSFGSLGAGDTELLKRLIAAIGRMPVRAL
                     VNVGDYKDQYTEIPDNVIVDSWFPQPSVIPLVDAVIHHGGNNSFTECLYFGKPALILP
                     YVWDGHDNATRVQETGHGFKMDRYNWTDAELADKVTAMLTDKKMKARLKATSKSMRAR
                     HGPTKAAKVIDSLARRKIA"
     gene            3889..4254
                     /locus_tag="PL152_08190"
     CDS             3889..4254
                     /locus_tag="PL152_08190"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HQR49302.1"
                     /translation="MPANLASSAPHLVAPESHPGPFVDWGVIPTMIEGESRTSGLVLH
                     KGPGGESETGLWICTPGYWHCHVTRDEFCHFLLGRCTYVHESGEIIEVVPGTVAFFPR
                     DWKGTCRVHETVRKVYMIR"
     gene            complement(4251..4700)
                     /locus_tag="PL152_08195"
     CDS             complement(4251..4700)
                     /locus_tag="PL152_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49303.1"
                     /translation="MTAVDERARIREALAAVRHCANTMDDQAASLLDALPTVKMDADL
                     RTSTAELSSNLKDVAARVTFELALLAPQVGAHESDVATVTQRLIDLDASMMEALAPVA
                     DLADRLESAAENDDAHEPAFVLVIDAAGALLQSLKKANAATAALQRS"
     gene            complement(4746..>5129)
                     /locus_tag="PL152_08200"
     CDS             complement(4746..>5129)
                     /locus_tag="PL152_08200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49304.1"
                     /translation="FFGEVSSAEFREAYLEMTADLRYAGIRYILSDYLDAAPGEDLTH
                     TFVGRVEELSRLQYECGPDIVHATVVAGEKMLAHVGCFESLPLAPYPEATFTTVEAAR
                     HWIAGNPRPAWRADLAAKNDGGAAA"
BASE COUNT          874 a         1789 c         1761 g          705 t
ORIGIN      
        1 tgaattccgc ggcgcaatga cgcccgcacc gcaccagcct ggtcaacccg atgtcaatcc
       61 tgtcacgcct gctaggcaac tccccgcctg cgacggaggt ctcgagcacg agcgctccgg
      121 ctgcgccggt cgaggcgccg cgtcccgatc ccgccgtgcg tgcgcgggaa gaggaagcca
      181 gcgtgtcgca ggcgatcgcg gccggcgaca tgacggcggt cggcacctgg gtcctcgatg
      241 gcagcacgac ccggatccgc cagatggcgg cacgtgccgt caccgacctc gagcagttgc
      301 gcgagctgat ccgggccacc cggggcggca acgacaagaa cgtccatcgc atcctcgcgg
      361 acaagcgcga cgagctgctc gcggagatgc gacacgcgca gcagctccag gccgatctcg
      421 agacgacggc ggcggacatc gcccagcaca gcgagcggcc gtacgacgcg ctgtattcgc
      481 cgactctcga acgactcgag gcgcagtggc gcgccctcga accgcagtct gcggagaccc
      541 tgcgcgattc ggtcgcacgg caactggaac gggcccgcgc ggtcgtcgac gaacatcgcc
      601 acgcgatcga agtcgaggcc gagcggaagc gctccgccgc gctcgccgcc gaggagttgc
      661 ggcgccagcg tgaactcgag gcacgagccg ccgccgaggc cgcggccgag gctgccgccg
      721 agcaggcgcg cgcgcgcgaa accgagcgac aggccgagat cacccgacag gaggcggagg
      781 cggccgaggt gcgccacctg ctgggactgc tgcgacaagc gcaggcagcc ctcgaccagg
      841 gcgggacggc gcgcgccgcg cgctttcgcg acgcgatcga ggaacggctg ccgagcgcgc
      901 cggccctgcc gccctggttc gcgcgcaagc tgcaggaggt cgcttcccgc atcgaggaat
      961 tgaaggactg gaagaccttc cgcgtcgtcc cgaagcgtgc ggaactggtc gagcggatgc
     1021 agtcgctcgt cggcgcggac atgtcgcccg aggagctcgc gcggcagatc cgccgcctgc
     1081 gggacgagtg gcggaccctc aatcgtggcg cggccgagga cccgtctccg gagtggcagg
     1141 cgttcgacga cgcggcgact cgcgcctacg agccctgccg cgagcacttt gcgcagcagg
     1201 ccgagcgacg cagggaaaac caggcgcacc gcgaggcgat gctcgagcgg ctctcggcgt
     1261 tcgccgcgca gcaggccggc gaggacgtca actggcgcgc ggtccagcag gccatcttcg
     1321 agtcgcgacg cgagtggcag cagtacgccc cggtggacca ggccgcggcc aggcccctgc
     1381 aggagcgatt ccgtgccgtg ctgcaggaac tgcagggtcg cctcgacgcc gagtacgccc
     1441 gcaacgtgca ggccaagcgc gaattgatcg cccggactgc ggcgctcgcg gacgtcaagg
     1501 acacgcggca ggccatagag gaagcgaaga gcctgcagcg gaagtggaag acggtcgggc
     1561 tcgtgccgcg acagcaggac aaccagctct gggaggagtt ccgccgccac tgcgacgccg
     1621 tgttcgagcg cagcgcgcag gaatgggccg cccacggcgc gtcgctcgac gccaaccagg
     1681 cacgggcgat cgcactgtgc gaggaactcg aagggcttgc cggatcggcc gacgaggcgc
     1741 cgccgtccga ccggcgaccg ctgggcgagg tgcgcgccga gttcgactcc ctggagcttc
     1801 cccgggcttc ggcgcgcgaa ctgcgccgtc gtttcgccga cgcggtgaac cgctacggcg
     1861 aagcagcccg ccggcaccgc gcggaagccg agcgtcgcgc ctggtccgac ttgttcgggg
     1921 tcggctcgcg gctgaacgaa tacgcactcg cggtggccca ggggcgctca cccgaggatt
     1981 gcgcggcatt tcgggaatcc gccgcttccg ccgtggccgg gctcgcgcac gcgcccaagg
     2041 gtacgcgcgc caccctggag cagcagatgg ccaggatcgc tgcaggcgag atcggcaccg
     2101 acctcgcggc gaacgaatcg gctcgacgga tgctatgcgt ccgcgcggaa ctgatcgcgg
     2161 gcgtgccgac gccgccagaa gacctggagc tgcgccgcga gtaccagatg caacgactgg
     2221 tcgcagcgat ggggcgcggg gaacgtccgg cgccggccga cctcgacgcc ctggcgctcg
     2281 agtggatcgc ggccgggccc gtcgaagcgg tcgtccacga cgcgctgctg gcccgcctgg
     2341 agcgctgtcg cggcacgctg ggcggtgctg cgtagacgag tcgtccggcg cgtctcccct
     2401 gccgcgaccg ctggcggcta tgccatgatc cgccaccgat gatcgccctg ctgcagggcg
     2461 ccgtgatgga gtcgatcccg tgccacgaac agcgaaaaag gcggcgaccc gcgcgagatc
     2521 gaccgcccgc aggaaacccg cggctcgcgc gaagccgtcg atccgcagca agcggccggc
     2581 acgcaagcga gacgggaggg tcatcgcgct gtttccggag gccgcattcg gtccggcact
     2641 gaattccgtg ggcatcggcc aggcgctcga gaagctcggg cacaaggtcg tgttcctgtc
     2701 cgatccgggc ttcctctccg tctacgaggg ctacgggttc gaggcgcatc cagtgaatct
     2761 ctccgagcct ctgccgccgg aagagatggc gaagttctgg gtcgacttca tcaacggcca
     2821 catcccgaac ttccgcaaga cgccgctcga gcaggtcgac aactacgtga aggactgctg
     2881 gggcatgatc gtgcagacgg cgaagtgggc cgagaaggac ctgccgggcg tgctcgagaa
     2941 agtccggccc gacgtgatct gcgtcgacaa cgtgatcctg ttccccgcca tcaagcgcta
     3001 cgcccgcaag cacaggatcc cgtgggtgag gatcatctcg tgctccgaaa acgagattga
     3061 ggatcccgac atcccaccgc atttgtctgg cgccggcgcc acggacagga aggcgcaggc
     3121 cgcgtatcgc aggcacttcc ggcaggtcat caagccgatc cacgaggagt tcaacgcgtt
     3181 cctgaaatcc gtcggcgagc gcaggtatcc gctgggccag ttcttcgagg cgagcccgca
     3241 catgaacctg ctgctctacc cgtccgcggt gaagttcaag cggcgccacc ggctgccggc
     3301 gcggcagttc cagtacctcg aaggctgcgt gcgcagggag aagccgtacc aggtcccgcg
     3361 cttcaaggcc aacgacgacc agcccctgct ctacgtgagc ttcggctcgc tgggcgcggg
     3421 agacacggaa ctcctgaagc gcctgatcgc cgcgatcggc cggatgccgg tgcgggcgct
     3481 ggtcaacgtc ggcgactaca aggaccagta cacggagatc cccgacaacg tgatcgtcga
     3541 cagctggttc ccgcagccct cggtgatccc gctcgtcgac gccgtgatcc accacggcgg
     3601 caacaactcg ttcaccgaat gcctctattt cggcaagcca gcattgatcc tgccgtacgt
     3661 ctgggacggc cacgacaacg ccacccgcgt gcaggagacg gggcacggct tcaagatgga
     3721 tcgctacaac tggacggacg ccgagctcgc cgacaaggtg acggcgatgc tcaccgacaa
     3781 gaagatgaag gcgcgcctca aggcgacgtc gaagtccatg cgcgccaggc acggcccgac
     3841 caaggccgcg aaggtgatcg actcgctcgc gcgacggaag atcgcctgat gccggccaat
     3901 ctcgcctcct ccgcgccaca tctcgtcgca cccgagtcgc atccgggacc gttcgtggac
     3961 tggggcgtca tcccgacgat gatcgagggc gagtcgcgga cgagcggact cgtgctgcac
     4021 aagggcccgg gcggcgagag cgagaccggc ctctggatct gcacgccggg ctactggcac
     4081 tgccacgtca cgcgcgacga gttctgccac ttcctgctcg gccgctgcac ctacgtgcac
     4141 gagtccggcg aaatcatcga agtggtgcca ggcaccgttg cgttttttcc cagggactgg
     4201 aagggcacgt gccgcgtgca cgagaccgtg cgaaaggtct acatgatccg ctaggagcgt
     4261 tgcagcgccg ccgtcgcggc gttcgccttc ttgagactct gcagcagggc accggccgcg
     4321 tcgatcacca acacgaatgc gggctcgtgc gcatcgtcgt tctcggcggc gctctcgagg
     4381 cgatcggcga ggtcggccac gggagccagc gcttccatca tcgacgcatc caggtcgatg
     4441 agccgctgcg tcacggtcgc cacgtccgat tcgtgggctc ccacctgcgg cgccagcagg
     4501 gccagctcga acgtcacgcg ggcggcgacg tccttcaggt tcgacgaaag ttccgcggtg
     4561 ctcgtccgca ggtccgcatc catcttcacg gtcggcagcg catcgagcag gcttgcggcc
     4621 tggtcgtcca tggtgttcgc gcagtgccgg acggcagcca gggcctcccg gatccgcgct
     4681 cgttcgtcaa ccgccgtcat cgctccctcc acgcccaccg gacacgcacc gccaggccac
     4741 ggccctcagg ccgcggctcc cccgtcattc ttcgccgcca ggtcggcgcg ccaggcaggg
     4801 cgcggattgc ccgcgatcca gtgccgcgct gcctccaccg tcgtgaacgt ggcttccgga
     4861 tagggcgcca gcggcagcga ctcgaagcag ccgacgtgtg ccagcatctt ctcgcctgcc
     4921 accaccgtgg cgtgcacgat gtccggaccg cactcgtact gcagccgcga cagctcttcg
     4981 acgcggccga cgaacgtgtg cgtcaggtcc tcgcccggcg cagcatcgag gtagtccgag
     5041 aggatgtagc ggatgccggc gtagcgcagg tccgccgtca tctcgaggta ggcctcgcgg
     5101 aactcggccg acgacacctc cccgaagaa
//
LOCUS       DAOMFD010000141         5115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6696_length_5115_cov_34.721542, whole genome shotgun sequence.
ACCESSION   DAOMFD010000141 DAOMFD010000000
VERSION     DAOMFD010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5115
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6696_length_5115_cov_34.721542"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..2318)
                     /gene="parC"
                     /locus_tag="PL152_08205"
     CDS             complement(63..2318)
                     /gene="parC"
                     /locus_tag="PL152_08205"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HQR49305.1"
                     /translation="MTTQNELNFEGIERLPLRSFTEKAYLEYSMYVILDRALPHVGDG
                     LKPVQRRIIYAMSELGLAATAKHKKSARTVGDVIGKFHPHGDSACYEALVLMAQPFSY
                     RYPVVDGQGNFGSPDDPKSFAAMRYTESKLTRYAEVLLSELGDGTVDWAPNFDGTLEE
                     PTLLPARLPNVLLNGATGIAVGMATDIPPHNLREVAAACVRLLEEPKATVAQLCEHVR
                     GPDFPTGAEIISPREELLRLYESGNGTFRARARYEIEDGEIVITELPYQVSGAKVLEQ
                     VANQMRAKKLPMVDDLRDESDHEHPTRLVITPRSNRVDVEQVMAHLFATTDLERSYRV
                     NLNVIARDGRPRVMGLKPLLEEWLTFRIDTVTRRLTHRLEKVEARLHILDGLMVAYLN
                     LDEVIRIVRYEDEPKAALIARFKLSEVQAEAILETKLRHLARLEEMKIRGEQDKLAAE
                     RDELAKTLASKAKLKRLVREEIIADAKTYGDDRRSRIVERAAAQAIAETELVASEPVT
                     VVLSKSGWVRAAKGHEIDPRSLSYKTGDEFRAVARGRSTQLAVFLDSTGRAYSLPAHT
                     LPSARGQGEPLSGRLDPPDGATFPGVLIGEPDERWVLAGSAGYGFVVKLGEMHSRNRA
                     GKAVLRVPDGTGVLAPVPVPAAEGALLAAVNSDGRLLVFPVSDLPELPRGKGNKIFGI
                     PTRKALSGEETLVAIAVVAPGQALRILSGERHMGLSFRELEQYRGERGQRGAVLPRGW
                     RKVDDLEVEVG"
     gene            complement(2335..4227)
                     /gene="parE"
                     /locus_tag="PL152_08210"
     CDS             complement(2335..4227)
                     /gene="parE"
                     /locus_tag="PL152_08210"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HQR49306.1"
                     /translation="MTQTYTAQDIEVLSGLEPVRRRPGMYTDTSRPNHLVHEVVDNSV
                     DEALSGHCSRISVTVHKDGSVEVTDDGRGMPVDIHPKEKVSGVELILTRLHAGGKFSD
                     KTYKFSGGLHGVGVSVVNALSKNLECWVRRGGKEYNISFANGKVKSKLEVVGEVGKAN
                     TGTTVRFWPDASYFDSAEISLPRLKHVLKAKAVLCPGLEMRLAVEKTGEKESWVYTGG
                     LEQYLRESLEGGEWLPDDPFTGSVEGEMEGVDWAVAWRTDDGTRTEESYVNLIPTLQG
                     GTHVNGFRLGLTEAIREFCEFRNLLPRGLKLAPEDVWDDASYVLSTKMRDPQFAGQTK
                     ERLTSREPAAFVSGIVKDQFGLWLNQHPEAGERIAQLAIGNAQSRLKASQKVARKRVV
                     SGPALPGKLADCTSQDPKRTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEV
                     DAGEILSSQEVHDIAVAIGVDPASDDVSALRYHKVCILADADSDGAHIATLLCALFLR
                     HFRPLVLAGHVFVAMPPLFRVDYGKQVAYALDEAERNGILDRIRTENPRTRPVVTRFK
                     GLGEMSALQLRETTMAPETRRLVQLTVDAKDHADQLMDMMLAKKRASDRREWLETKGN
                     LAGVQP"
     gene            complement(4696..>5115)
                     /locus_tag="PL152_08215"
     CDS             complement(4696..>5115)
                     /locus_tag="PL152_08215"
                     /inference="COORDINATES: protein motif:HMM:NF023432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3501 family protein"
                     /protein_id="HQR49307.1"
                     /translation="QEMLRIERIFEPEGIQSELDAYNPLIPDGSNWKATLLIEFPDAE
                     ERREALARLKGIEGRCWMRVEGHARVPAIADEDLERENEDKTSAVHFMRFELTPEMVA
                     AAKAGAAVSLGMDHEQYRHAVEPLPEASRASLVRDLA"
BASE COUNT          706 a         1823 c         1723 g          863 t
ORIGIN      
        1 ttcgtgtcga gccccgagga agtcgtgctc gaagccaggt gagccgtgac caacggcacc
       61 cgttagccga cctccacttc caggtcgtcc accttccgcc atccccgcgg caatacggcg
      121 ccgcgctggc cgcgctcccc gcggtactgc tcgagttcgc ggaagctgag ccccatgtgg
      181 cgctcaccgg acaggatgcg cagcgcctgc cctggcgcga ccacggcgat cgcgacgagg
      241 gtctcctcgc cggacagcgc cttccgggtc gggatgccga agatcttgtt gcccttgccg
      301 cgtggcaact ccgggaggtc ggacacgggg aacacgagca ggcggccgtc cgagttcacg
      361 gccgcgagca gcgcaccctc ggccgccggc accggcaccg gcgccagcac gcccgtgccg
      421 tccggcaccc tgagcacggc cttgcccgcg cggttgcggc tgtgcatctc cccgagcttc
      481 accacgaatc cgtaacccgc gctgcccgcg agcacccagc gctcgtccgg ctcgccgatc
      541 aggactccgg gaaacgtcgc gccgtccggc gggtccaggc ggcccgatag cggctcgccc
      601 tgccctcgcg ccgacggcag cgtgtgcgca ggcaggctgt aggcgcgtcc ggtgctgtcg
      661 aggaacaccg cgagctgcgt gctgcggcca cgcgccaccg cacggaactc gtcgcccgtc
      721 ttgtacgaca gcgagcgcgg gtcgatctcg tgacccttgg ccgcgcgaac ccagccgctc
      781 ttcgacagca ccaccgtgac cggctcgctt gcgacgagct cggtctccgc gatcgcctgc
      841 gcggccgcgc gctcgacgat gcgactgcgg cggtcgtcgc cgtaggtctt cgcgtccgcg
      901 atgatctcct cgcgcaccag tcgcttcagc ttcgccttgc tggccagcgt cttcgcgagt
      961 tcgtcgcgct ccgccgcgag cttgtcctgc tcgccgcgga tcttcatctc ctcgagcctg
     1021 gcgaggtgtc gcagcttggt ctcgaggatc gcctcggcct gcacctccga cagcttgaag
     1081 cgcgcgatca acgccgcctt cggctcgtcc tcgtagcgga cgatgcggat cacctcgtcg
     1141 aggttcaggt acgcgaccat caggccatcg aggatgtgca gccgcgcctc gaccttctcg
     1201 agccggtgag tcagccggcg cgtgaccgtg tcgatgcgga acgtcagcca ctcctcgagc
     1261 agcggcttca gccccatgac gcgcgggcga ccgtcgcgcg cgatcacgtt gaggttcacg
     1321 cggtagctgc gctcgaggtc ggtggtggcg aagaggtgcg ccatcacctg ctcgacgtcc
     1381 acgcggttgg agcggggcgt gatcacgagc cgcgtcgggt gctcgtggtc ggactcgtcg
     1441 cgcaggtcgt cgaccatcgg cagcttcttg gcgcgcatct ggttcgcgac ctgctcgagg
     1501 accttggccc ccgacacctg gtacggcagc tcggtgatga cgatctcgcc gtcctcgatc
     1561 tcgtagcgtg cgcgggcccg gaacgtgccg ttgcccgact cgtacaggcg cagcagctct
     1621 tcgcgcgggg agatgatctc ggcgccggtc gggaagtccg gccctcgcac gtgctcgcag
     1681 agctgcgcca ccgtggcctt cggctcctcg agcagccgca cgcaggcggc ggccacttcc
     1741 cgcaggttgt gcggcgggat gtcggtcgcc atgccgaccg caatgccggt cgctccgttc
     1801 agcagcacgt tcggcaggcg cgccggcagc agcgtcggtt cctcgagcgt gccatcgaag
     1861 ttcggcgccc agtccaccgt gccgtccccg agttcggaga ggagcacttc ggcgtagcgc
     1921 gtgagcttgg actcggtgta gcgcatcgcc gcgaacgact tcgggtcgtc gggcgaaccg
     1981 aaattgccct gcccgtccac gaccggatag cggtacgaga acggctgcgc catcagcacc
     2041 agggcctcgt agcaggcgga gtcgccgtgc ggatggaact tgccgatcac gtcgcccacg
     2101 gtgcgggcgg acttcttgtg cttggccgtg gccgcgaggc cgagctcgct catcgcgtag
     2161 atgatgcgtc gctgcaccgg cttcaacccg tccccgacgt gcggcagcgc gcgatcgagg
     2221 atcacgtaca tcgagtactc gaggtacgcc ttctcggtga aggacctgag cggcagccgc
     2281 tcgatgccct cgaagttgag ttcgttctgc gtcgtcacgg gatctcctgc ccgctcaggg
     2341 ctgcacgccg gccaggttgc ccttggtctc gagccattcg cgtcggtcgg aggcgcgctt
     2401 cttcgcgagc atcatgtcca tcaactggtc cgcgtggtcc ttcgcgtcca cggtgagctg
     2461 caccagccgg cgcgtctcgg gcgccatggt cgtctcgcgc agctgcagcg cgctcatctc
     2521 gccgagcccc ttgaaccgcg tcacgacggg ccgggtccgc gggttctcgg tgcggatgcg
     2581 gtcgaggatg ccgttgcgct cggcctcgtc gagcgcgtag gcgacctgct tgccgtagtc
     2641 gacgcggaac agcggcggca tggcgacgaa cacgtggccg gcgagcacca gcggccggaa
     2701 gtgccgcagg aacagcgcgc agagcagcgt ggcgatgtgc gccccgtcgg aatccgcgtc
     2761 ggcgaggatg cagaccttgt ggtagcgcag cgccgacacg tcgtccgacg ccgggtccac
     2821 gccgatcgcg acggcgatgt cgtgcacttc ctgcgacgag aggatctcgc cggcgtccac
     2881 ttcccacgtg ttcaggatct tgccgcgcaa gggcatgacc gcctggaatt cgcggtcgcg
     2941 ggcctgcttc gccgagcctc ccgcggagtc tccctcgacc aggaacagct cggtgcgctt
     3001 cgggtcctgg ctcgtgcagt ccgcgagctt gcccggcagc gccggcccgc tcacgacgcg
     3061 cttgcgcgcg accttctggc tcgccttcag ccggctctgc gcgttgccga tggcgagctg
     3121 cgcgatgcgc tcgccggcct cggggtgctg attgagccac agcccgaact ggtccttgac
     3181 gatgccggag acgaacgccg cgggctcgcg cgaggtcagt cgctccttgg tctgcccggc
     3241 gaactgcggg tcgcgcatct tggtcgagag cacgtagctc gcgtcgtccc agacgtcctc
     3301 cggcgcgagc ttcaggccgc gcggcaggag gttccggaac tcgcagaatt cgcggatcgc
     3361 ctcggtgagc ccgaggcgga agccgttcac gtgcgtgccg ccctgcagcg tcggaatcag
     3421 gttgacgtag ctctcctcgg tgcgcgtgcc atcgtcggtg cgccacgcga ccgcccagtc
     3481 gacgccttcc atctcccctt cgacgctgcc cgtgaacgga tcgtccggca gccactcccc
     3541 gccctccagc gactcgcgca ggtattgctc gaggccgccc gtgtagaccc acgactcctt
     3601 ctcgcctgtc ttctcgaccg cgaggcgcat ctcgaggccc gggcacagca ccgccttggc
     3661 cttgagcacg tgcttcaacc tcggcagcga gatctcggcg ctgtcgaagt acgacgcgtc
     3721 gggccagaag cgcacggtcg tgccggtgtt ggccttgccg acctcgccca cgacctcgag
     3781 cttcgacttc acctttccgt tggcgaagga gatgttgtat tccttgccgc cgcggcgcac
     3841 ccagcactcg aggttcttcg acagcgcatt gacgaccgac acgccgacgc cgtgcagtcc
     3901 tccggagaac ttgtaggtct tgtcggagaa cttgccgccg gcatgcagcc gcgtgaggat
     3961 gagctcgacg ccgctcacct tctccttcgg gtggatgtcg accggcatgc cacggccgtc
     4021 gtcggtgact tcgacggacc cgtccttgtg cacggtgacc gagatgcggc tgcaatggcc
     4081 ggaaagcgcc tcgtccacgc tgttgtccac gacctcgtgg accaggtgat tcggccggct
     4141 cgtgtcggtg tacatgcccg gccggcgccg gacgggctcg agtccgctca ggacctcgat
     4201 gtcttgagcg gtgtaggtct gcgtcatcgt gtggcgttcc gcgggctcgc gtcgagaaag
     4261 gcgcgcggat tctagcagcg cggtcccgtc gtcgcacgcg acggcgcgtc gcgcgtcgca
     4321 gcctcgcgac acctgtcgaa gccgctggtc gctactcacg cattaacagc gccgggcagg
     4381 cgtcgccacg aggagcttgc ccgcgaaaac gccgtaaacc catccctggg ggctccgggc
     4441 aaggcccatg gagcagacgg tttcgcggcc aggaccgacg tgccgacgcc tcggaggcgc
     4501 ggcacagccg cgccagagat tgagctggcc agcgtcgcgt ttcgcgaggc ggcgcgcggc
     4561 gacgcgaacg ctcggcgggc gggcatccag accgcatccg gcgggcgtca cgcggcatgg
     4621 acgccgcgtg cgagccccca aggacgggtt tacggcgtcc cgccggatgc ggtctggatg
     4681 cccgcccgct gactgctacg cgagatcgcg gacgagcgac gcgcgcgaag cctccggcag
     4741 cggctcgacc gcgtgccggt actgctcatg gtccatcccg agcgagaccg ccgcgcccgc
     4801 cttcgcggct gcgaccatct ccggcgtgag ctcgaagcgc atgaagtgca cggcggacgt
     4861 cttgtcctcg ttctcccgct cgagatcctc gtcggcgatc gccggcacgc gcgcgtgccc
     4921 ctcgacccgc atccagcagc ggccctcgat gcccttgagc cgcgcgagtg cctcgcgccg
     4981 ctcctccgcg tcgggaaact cgatgaggag cgtcgctttc cagttcgagc cgtcgggaat
     5041 gagcgggttg taggcgtcga gctccgactg gatgccctcg ggctcgaaga tgcgctcgat
     5101 gcgcagcatc tcctg
//
LOCUS       DAOMFD010000142         5106 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6719_length_5106_cov_32.972481, whole genome shotgun sequence.
ACCESSION   DAOMFD010000142 DAOMFD010000000
VERSION     DAOMFD010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5106
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6719_length_5106_cov_32.972481"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            275..709
                     /gene="fur"
                     /locus_tag="PL152_08220"
     CDS             275..709
                     /gene="fur"
                     /locus_tag="PL152_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004753949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HQR49308.1"
                     /translation="MVESSQLRRAGLKITTPRLKILDILAGSPLRHMSAEDVYRRLIE
                     LNEDIGLATVYRVLTQFEGAGLVTRHHFEGGTAVFELNEGTHHDHIVCMDCGKVEEFT
                     DEGIEERQRAIAKRLGFNLADHALTLHGHCVRENCPNRARKR"
     gene            complement(651..>1914)
                     /locus_tag="PL152_08225"
     CDS             complement(651..>1914)
                     /locus_tag="PL152_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HQR49309.1"
                     /translation="AYQSWRALEQARAEFDERGRDRAQRLEMLSHYVAELDALDAKAG
                     EAASLNEERRRMASLGRLAEGTRQVCDLLAADGSGASDALARSQSILKQLVSLDATLS
                     PLSALLDEASISCREALAGLDRYVDSLEADPARQERVESRLAALEAAARKHRVEVDEL
                     PELRQRLADELAGLGEATAGVAELERRTAAALAAYESAARTLSERRRAAAAILDREVS
                     ALMQELGMRGGVFATRIETPSPLPRSEHGADEIEFFVSANPGQPPKPLAKTASGGELS
                     RISLALQVATLDASHLPCLVFDEVDAGVGGAVAEMVGRQLRALAESGQVLCVTHLPQV
                     AAQASHQVRVVKRTQGGVTRTALEALDGDGRIEELARMLGGARVTDRAREHAREMLES
                     NRLRARSRPASVSGRDSGSSRARSGRGA"
     assembly_gap    1915..2014
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2015..2172)
                     /locus_tag="PL152_08230"
     CDS             complement(<2015..2172)
                     /locus_tag="PL152_08230"
                     /inference="COORDINATES: protein motif:HMM:NF024867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR49310.1"
                     /translation="MLTHLQIRDFAIVEAVEFEPAPGFTALTGETGAGKSILVDALLL
                     AVGGRADSG"
     gene            complement(2181..3089)
                     /locus_tag="PL152_08235"
     CDS             complement(2181..3089)
                     /locus_tag="PL152_08235"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HQR49311.1"
                     /translation="MSTGTTAVRTVALAGRPEEPRVAEAMQVLASHLTGRGVRVRAAT
                     GGHVDYGPHEIELVAEPELVGGVDFVVAVGGDGTMLHAARAAAAHDVPVLGINRGRLG
                     FLADVGPARMLESVDDALEGRCVPERRMLLQATVSGAGAPRAAIALNDVAVAKHDTGR
                     MLDLQTWVDGAFVNTHAGDGFIVSTSTGSTAYALSCGGPIVHPALAAIVLVPICPHTL
                     SDRPIVVGADSVVELELAGRPETRAQVVCDGIPIYELEPGLRLRVERATVSATLLHPP
                     DHDYFRILRSKLHWGRGGRSGRPPAD"
     gene            3244..4311
                     /gene="hrcA"
                     /locus_tag="PL152_08240"
     CDS             3244..4311
                     /gene="hrcA"
                     /locus_tag="PL152_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HQR49312.1"
                     /translation="MAPETSEDIINERAQHLLRVLVESYIRDGQPVGSRALSRDSGLN
                     LSSATVRNVMADLEEYGFVTSPHTSAGRVPTDKGYRFFVDTLLRQRPAAAAEEVIDAL
                     RRRLDEEPERDPKTIVSVASQVLSSITHLAGVVTIPRQPHQALSQIEFLPLSGNRVLA
                     VMVVNGRDVQNRIVELERYYSADELRRAAAFLNQQFAGKELRQVREDLVGELAQAGEQ
                     LNQLMRDAIMLARQMVGPAAPEASEYIIAGETNLMEFAELSNVDKLRRLFEAFTAKRD
                     ILHLLDLSLRAEGVQIFIGQESGYQILDDCSLVTAPYMLDNETVGVLGVIGPTRMAYE
                     RVIPIVDVTAKLLGSALNSRR"
     gene            4390..4938
                     /gene="grpE"
                     /locus_tag="PL152_08245"
     CDS             4390..4938
                     /gene="grpE"
                     /locus_tag="PL152_08245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HQR49313.1"
                     /translation="MTNESVQDSQVEPAAGTTSEVERLTAELALAQGKAAEHYDAYVR
                     AVAEAENVRRRAARDVESASRYAIERFAAELLEVRDSLELGIAAGAGADPARLVEGME
                     ATLRLVNRAFEKSGISVVDPQGQPFNPEFHEAMVTQPTADQPPGTVLAVVQKGYVLNG
                     RLLRPARVVIARAPEPVAEQPG"
     gene            5009..>5106
                     /locus_tag="PL152_08250"
     CDS             5009..>5106
                     /locus_tag="PL152_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQR49314.1"
                     /translation="MARVIGIDLGTTNSCVAIMDGGKPRVIENSEG"
BASE COUNT          778 a         1792 c         1720 g          716 t
ORIGIN      
        1 ccgaggatgt agcgcacctg gctgcgggtc atgccgacct tgacctgctc gatctcgtcg
       61 agctgcagga ggttgccctg ctggatcgtc ggccggtaga cgcacccggc ggtggccatg
      121 ctgccaagta ccgcagcgag gaccgctacg cggcgcatgc gggaaaatac gtgggtcgac
      181 tgcatctgtg tggagttgcc ggcccgggcc ggtgataatg gccgcggatc atacaggaag
      241 ccccgccctc gcgcgggcgc ccgaggaaat gacggtggtt gaatcctcgc aattgcgccg
      301 ggccggcctc aagatcacta cgccgcgcct caagatcctc gacatcctcg cgggcagccc
      361 gctgcgtcac atgagcgccg aggacgtgta tcgccgcctg atcgagctga acgaggacat
      421 cggcctcgcc accgtgtacc gcgtcctgac gcagttcgaa ggcgccggcc tcgtgacgcg
      481 acaccacttc gagggcggca cggcggtctt cgaactcaac gaaggcacgc accacgacca
      541 catcgtctgc atggactgcg gcaaggtcga ggagttcacc gacgaaggca tcgaagagcg
      601 ccagcgtgcg atcgccaagc gcctcggctt caatctcgcg gaccacgcgc tcacgctcca
      661 cggccactgc gtgcgcgaga actgcccgaa tcgcgcccgg aaacgctagc cggacgcgac
      721 cgggctcgca gccggttgga ttccagcatt tcgcgggcgt gctcgcgcgc gcggtccgtg
      781 acccgtgcgc cgccgagcat gcgggccagt tcctcgatgc gaccatcacc atccaatgcc
      841 tcgagggcag tgcgcgtcac gccgccctgc gtgcgcttga cgacacgcac ctggtgactg
      901 gcctgggccg cgacctgcgg caggtgcgtc acgcacagga cctgtcctga ctccgccagc
      961 gcccgcagtt gccgtcccac catttccgcc accgccccgc cgacgccggc atcgacttcg
     1021 tcgaacacca ggcaggggag gtgcgacgcg tcgagggtcg cgacctgcaa tgccaggctg
     1081 atgcgggaca gctcgccccc cgaagcggtc ttcgcgaggg gcttgggggg ctgtccgggg
     1141 ttggcgctca cgaagaattc gatctcgtcg gcgccgtgct ccgaccgcgg cagcggcgac
     1201 ggggtctcga tgcgcgtcgc gaataccccg ccgcgcatgc cgagctcctg catcaacgcg
     1261 ctgacttcgc ggtcgaggat cgcagccgca gcgcgacgcc gctccgacaa cgtccgcgcc
     1321 gcggactcgt acgccgcaag tgcggcggcc gtgcgacgtt cgagttcggc cacaccggcc
     1381 gttgcctcgc cgagacccgc cagttcgtcg gcgagccgct ggcgcagctc cgggagttcg
     1441 tccacctcga cgcggtgctt gcgcgcggcc gcctcgagtg ccgccagcct cgattccacg
     1501 cgctcctgcc gggccgggtc agcctcgagg gagtcgacgt agcggtccag gcccgccagc
     1561 gcctcacggc acgagatgct ggcctcgtcg agcagggccg acaacggcga cagggtcgcg
     1621 tcgagcgaca cgagctgctt caggatcgac tgcgatcgtg caagcgcatc cgacgcgccg
     1681 ctgccgtcgg cggccagcag gtcgcagact tgccgcgttc cctcggccag gcgacccagg
     1741 ctcgccatgc ggcggcgttc ctcgttgagg cttgccgcct cgccagcctt cgcgtccagc
     1801 gcatcgagct cggccacgta gtgcgacagc atctccagcc gctgcgcgcg atcgcggccg
     1861 cgctcgtcga actccgcgcg cgcctgctcg agcgcgcgcc acgactgata cgccnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnccgctg tcggcccggc cacccacggc
     2041 gagcagcagc gcgtcgacga ggatggactt gccggcaccg gtttcgccgg tcagggccgt
     2101 gaagccgggc gcgggctcga actcgacggc ctcgacgatc gcgaagtcgc gaatctgcag
     2161 gtgcgtgagc atgggccggc tcagtcggcg ggcggccgac cgcttcttcc gccgcggccc
     2221 cagtgcagtt tggagcgcag gatgcggaag tagtcgtggt cgggcgggtg cagcagcgtc
     2281 gcactcacgg tggcgcgctc gaccctgagc cgcaggcccg gctcgagttc gtagatcggg
     2341 atgccgtcgc acaccacctg ggcgcgcgtc tccgggcggc ccgcgagctc gagctcgacg
     2401 acgctgtcgg cgccgaccac gatcggtcga tccgacaacg tgtgcgggca gatcggcacc
     2461 agcacgatgg ccgccagggc cgggtgcacg atcgggccgc cgcaggacag cgcgtaggcc
     2521 gtcgatcccg tggacgtgga gacgatgaac ccgtcgcccg catgcgtgtt gacgaatgcg
     2581 ccgtccaccc aggtctgcag gtcgagcatg cgcccggtat cgtgcttggc caccgcgacg
     2641 tcgttgagcg cgatcgccgc gcgtggcgcg cccgctccgc tgaccgtcgc ctggaggagc
     2701 atgcgccgct ccggcacgca ccggccctcg agcgcatcgt ccacggactc gagcatccgc
     2761 gccggtccga cgtcggcgag gaagccgagc cggccgcgat tgatgcccag caccgggaca
     2821 tcgtgcgcgg cggccgcgcg cgcggcatgc agcatggtgc cgtcgccgcc gaccgccacc
     2881 acgaagtcga cgccgcccac gagctcaggc tccgcgacga gttcgatctc gtgcgggccg
     2941 tagtcaacgt gcccgccggt ggcggcacgc acgcgcacgc cgcgcccggt caggtgggac
     3001 gcgaggactt gcatggcctc ggccacgcgt ggctcctcgg ggcgacccgc cagcgcgacg
     3061 gtccggacag ccgtggttcc ggtgctcatg ggtgcgcgtc ttttagcacg gccgccgtgc
     3121 gcgccgccat cgcgcggaat gtcgcaaatc ccgcgaatcg ctgtgcttga cttgggcaaa
     3181 accgcgttgc taggttggtt gccgcaagcc gggcggcgtt tcgcccggcg gccccgagca
     3241 acgatggcgc cggaaacttc agaagacatc atcaacgagc gtgcccagca cctgctgcgc
     3301 gtcctcgtcg agagctacat ccgcgacggc cagccggtcg ggtcgagggc gctgtcgcgg
     3361 gattcgggcc tgaacctgag ctcggcgacc gtgcgcaacg tcatggccga cctcgaggag
     3421 tacggcttcg tcacttcgcc ccacacctcc gcaggccgtg tgcccacgga caagggttac
     3481 cggttcttcg tcgacacgct gctccggcag cggcccgcgg cggcggcgga ggaggtcatc
     3541 gacgcgctgc gccggcggct cgacgaggaa ccggagcggg acccgaagac catcgtgtcg
     3601 gtcgcctccc aggtgctgtc gtcgatcacg cacctcgccg gcgtggtgac catcccccgc
     3661 cagccgcacc aggcgctcag ccagatcgag ttcctgccgc tgtcgggcaa tcgcgtgctg
     3721 gcggtgatgg tcgtcaacgg ccgcgacgtg cagaaccgca tcgtcgaact cgagcgctat
     3781 tactcggcgg acgagttgcg gcgggcggcc gcgttcctca accagcagtt cgccggcaag
     3841 gagctgcggc aggtccggga ggacctcgtc ggcgagctcg cgcaggccgg cgagcagctg
     3901 aaccagctga tgcgcgacgc gatcatgctg gcccggcaga tggtcgggcc cgcggcgccg
     3961 gaagcgtccg agtacatcat cgccggcgag accaacctga tggagttcgc cgagctctcc
     4021 aacgtggaca agctccgccg gctgttcgag gcgttcacgg cgaagcgcga catcctgcac
     4081 ctgctcgacc tgagcctgcg cgccgaaggc gtgcagatct tcatcgggca ggagtccggt
     4141 taccagatcc tcgacgactg cagcctcgtg accgcgccgt acatgctgga caacgagacc
     4201 gtcggggtcc tcggcgtcat cggcccgacc cgcatggcct acgagcgcgt catcccgatc
     4261 gtggacgtga cggcgaagct gctcggctcg gccttgaatt cccggcgcta gccccgatat
     4321 cgacggcacc cgtgcccggc ggccggctga cgccggaccg cgggtgcgcc cgacatgcag
     4381 gcaggtgaca tgacgaacga atccgtccag gacagccagg tcgagcccgc ggcgggcacg
     4441 acatccgaag tcgagcggct gaccgcggag ctggccctcg cccagggcaa ggccgccgag
     4501 cactacgatg cctatgtccg tgcggtcgca gaggccgaga acgtccgccg gcgcgccgcg
     4561 cgcgacgtgg agtccgccag ccgctacgcg atcgagcggt ttgccgcgga gctgctcgag
     4621 gtccgcgaca gcctggaact cggcatcgcc gctggggcag gcgccgatcc tgcccgtctc
     4681 gtcgagggca tggaagcgac gctgcggctg gtcaaccgcg cgttcgagaa gtccggaatc
     4741 tcggtggtcg atccgcaggg ccagccgttc aacccggaat tccacgaggc catggtgacc
     4801 cagccgaccg cggaccagcc gcccggcacg gtgctggccg tggtccagaa gggctatgtc
     4861 ctgaacgggc gcctgctccg gcccgcccgc gtcgtcatcg cccgggcgcc agaacccgtc
     4921 gcggagcagc ccggctgact tgaatcaccc gccgcggacc cccacctagg cagtcatcga
     4981 catccacaga accgacggag tcccttccat ggcaagagtc atcggcatcg acctgggcac
     5041 gaccaactcg tgcgtcgcca tcatggatgg cggcaagccc cgcgtgatcg agaacagcga
     5101 gggtga
//
LOCUS       DAOMFD010000143         5099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6731_length_5099_cov_46.587629, whole genome shotgun sequence.
ACCESSION   DAOMFD010000143 DAOMFD010000000
VERSION     DAOMFD010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6731_length_5099_cov_46.587629"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1679
                     /gene="traD"
                     /locus_tag="PL152_08255"
     CDS             <1..1679
                     /gene="traD"
                     /locus_tag="PL152_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type IV conjugative transfer system coupling
                     protein TraD"
                     /protein_id="HQR49315.1"
                     /translation="EARLPANRPLLRGGPLAGVLTGFVPAADSSSVGGDPAIHGVEPD
                     ESEIWMDLEDRVGHTLVLGTTRVGKTRLAELLIAQDIRRGEVVVVFDPKGDVDLLRRV
                     FAEAERAGRAGEFFMFHLGHPEISARYNPVGSFSRITEVATRIAGQLPSEGQSAAFKE
                     FVWRFVNVMARALVALGRKPDYQEINRYASDVEPLLIDYFEYWLDREPSATGWREELR
                     SLAIDKKNLDKGLQSRGARAVSLVEYARRKKLYDPIAHALASTLNYEKSHFDKLVASL
                     LPLMEKLTTGRTASLLSPELDDPTDWRPVFDWTSVIHLGGIVYVGLDALSDYEVAAAV
                     GNSMFADLTSVAGSLYKFGAGRGLPGEVTPRRIAIHADEFNELIGDEFIPLLNKAGGA
                     GFQVTAYTQTWSDVEARIGSPAKAGQIAGNFNTLIMLRVKEVATAELLTSQLPEVHVV
                     STVVSSSVSDTNDPIDFADFASRNEDRIAPETVRMLEPTDLVQLPKGEAFALIHGGQL
                     HKIRMLLPSPASDPLMPASLAAIGKTLRARLDGPWNQPEIGFEAEERRVV"
     gene            1669..2382
                     /locus_tag="PL152_08260"
     CDS             1669..2382
                     /locus_tag="PL152_08260"
                     /inference="COORDINATES: protein motif:HMM:NF025706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4400 domain-containing protein"
                     /protein_id="HQR49316.1"
                     /translation="MSSRNAAFHDGALAGVLLSPLKLAFSLALGLVALLLCAWIVDWV
                     FVFKVWPQGIERLRELLAHDLEHGVALAARQGAGAGAITAPANALYSFVFEATGIHDM
                     GTQFANGAALSIPDTVMRRSYVSHRESIEAAMVGTQLLGVRAAILARFAPLLLLLYAV
                     GAADGFTQRAIRRACGGRESAGLYHRAKYLQVAALGLGGVSLLLWPGPVRWELCVPLG
                     AVLLGGLASVQWAYYKKHM"
     gene            complement(2422..3612)
                     /locus_tag="PL152_08265"
     CDS             complement(2422..3612)
                     /locus_tag="PL152_08265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49317.1"
                     /translation="MRRQGTPVTREALYEEVWTDAVTVVAPRYGLSDVGLVKICKKLG
                     IPVPPRGYWAKVKAGRPTRKVPLPALPAGARDLAGPIPLSEQEAATHARVRDALQQTR
                     ESQPSVSVPDELVDPHPLLRAAAARLKRRDGWDHPAGVRSASKEVLDLQVTRNALDRA
                     LRLADTLLKALEPSGFTARVDEEKGETLLVGGGTTLTISIVEQVTRTSHTPTLAEVRA
                     RDRYYDSFRVGARGEYPHIPQFDWHPTGRLTITVGSWPSRRWNDTERSFIDSRLGGIV
                     AAIVGLAEAKCAKEEEEERRRRTYEEARARYEAQVRARNEERRQLRSLFRDASRLQRA
                     NRLREFIAAVEDRAHRDGELTQEKQQWIEWARAKADWIDPLVRRSDPILDAPEPEAPS
                     FWRF"
     gene            3759..4838
                     /locus_tag="PL152_08270"
     CDS             3759..4838
                     /locus_tag="PL152_08270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49318.1"
                     /translation="MLQAQASERELIDQFLAALRSLPEVRAELEGPGAPELDAQLALD
                     VAGKPIHALVELKRAVYPRDVRQLVWRIRDLARQQPAGESGGEPLAVLVAESISPGAK
                     ELLRAERVGYYDSGGSLYLPARGAYLYVDRPPPKSLSRSMRSLFTGRRAQVLHGLLVR
                     HQDWFGVKDLAEQTLVSSATASQVLTEMERFDWLESRGQGPSKQRHLRQPAALLDAWA
                     KQLGSIRAPALRRYFVPAMKPDGLMERLGQVCEARGVEYSVSFEAAAQRYAPFLSAVS
                     QVRCRLLAGSGADAAIADLGARVVSEGANLSVIEAKSTGDLLFRERVGGVWLASPIHV
                     YLDLLRGEGRAKEMADHLRKERIGF"
     gene            4838..>5099
                     /locus_tag="PL152_08275"
     CDS             4838..>5099
                     /locus_tag="PL152_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016448853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HQR49319.1"
                     /translation="MAKPATLDGYSDQYTLDCERVLVTLLRGLGPWKDSVYLVGGLTP
                     RYLVPARPPVVPAHAGTLDVDIVIDLQILADTEAYHTLEENLK"
BASE COUNT          741 a         1716 c         1779 g          863 t
ORIGIN      
        1 acgaagcacg cctgccggcg aaccggccgc tccttcgagg cggcccgctc gcgggtgtat
       61 tgaccggctt cgtgccggcg gcagattcaa gcagtgtcgg cggggatccc gccatccacg
      121 gggtcgaacc cgacgagagc gagatctgga tggatctgga agaccgcgtc ggacacacgc
      181 tggtgttggg aaccacgcgg gtcggcaaaa cgcgcctcgc cgagctgttg atcgcccagg
      241 acatccgccg cggcgaggtt gtggtcgtgt tcgaccccaa gggcgacgtc gaccttctgc
      301 gccgtgtgtt cgcggaagcc gagcgggccg gacgtgccgg ggagttcttc atgttccacc
      361 tcggccatcc ggagatctcg gcgcgctaca acccggtggg cagcttctcg cgcatcaccg
      421 aggtcgccac ccgcatcgcg ggccagttgc cgtcggaagg ccagtccgcc gcgttcaagg
      481 agttcgtctg gcgcttcgtg aacgtgatgg cccgtgcgct cgtggcgctc ggtcgcaagc
      541 ccgactacca ggagatcaac cgctacgcgt ccgacgtcga gccgctgctg atcgactact
      601 tcgagtactg gctcgaccgc gagccctccg ccacgggctg gcgcgaggag ctgcgatcgc
      661 tggccatcga caagaagaac ctcgacaagg gtctgcagtc gcgcggcgcg cgggcggtga
      721 gcctggtgga gtacgcgcgg cgcaagaagc tctacgaccc gatcgcccac gcgctcgcct
      781 cgacgctcaa ctacgagaag agccacttcg acaagctggt ggcgtcgctg ctgccgctga
      841 tggagaagct cacgacgggg cgcacggcct cgctgctctc gcccgagctc gacgatccga
      901 ccgactggcg cccggtgttc gactggacct cggtgatcca cctgggcggc atcgtctacg
      961 tcgggctcga tgcgctctcc gactacgagg tggccgcagc ggtggggaac tccatgttcg
     1021 ccgacctcac cagcgtggcc ggcagcctct acaagttcgg cgccggtcgc ggcctgccgg
     1081 gcgaggtgac gccccggcgg atcgcgatcc acgccgacga gttcaacgag ctgatcggcg
     1141 acgagttcat cccgctgctc aacaaggcgg gcggggcggg gtttcaggtg acggcctaca
     1201 cgcagacctg gtcggacgtg gaggcgcgca tcggctcgcc ggccaaggcc gggcagatcg
     1261 ccggcaactt caacacgctg atcatgctgc gggttaagga ggtggcgacg gccgagctgc
     1321 tgaccagcca gctgcccgaa gtgcacgtcg tctcgaccgt cgtgtcgtcg tcggtctccg
     1381 acaccaacga cccgatcgac ttcgcggact tcgccagccg caacgaggac cgcattgcgc
     1441 cggagacggt tcggatgctc gaaccgaccg acctggtgca actgcccaag ggcgaggcct
     1501 tcgcgctgat ccacggcggc cagctgcaca agatccgcat gctgctgccg tcgcctgcga
     1561 gcgatccgtt gatgccggcc agcctcgcgg cgatcgggaa gacgctgaga gcgcggctcg
     1621 acggaccgtg gaatcagccg gaaattggct tcgaggcgga ggagcggcgt gtcgtctagg
     1681 aacgcggcgt ttcacgacgg ggcgctggcg ggcgtgctgc tgagcccgct gaagctggcc
     1741 ttctcgctgg ccctgggtct ggtcgcgctc ctgctgtgcg cctggatcgt cgactgggtg
     1801 ttcgtgttca aggtctggcc gcaggggatc gagcgcctgc gtgaactgct cgcgcacgac
     1861 ctcgagcacg gcgttgccct tgccgcccga cagggtgccg gcgccggcgc catcaccgcg
     1921 ccggccaacg cgctgtattc gttcgtgttc gaagcgactg gcatccacga catgggcaca
     1981 cagttcgcca acggggcggc tctgtcgatc ccggacacgg tcatgcggcg cagctacgtg
     2041 tcgcaccggg agagcatcga ggcggcgatg gtcggcacgc agttgctcgg cgtgcgcgcg
     2101 gcgatcctgg cgcggttcgc gccgctgctc ctgctcctct acgccgtcgg cgccgccgac
     2161 ggcttcacgc agcgagcgat ccgccgggct tgcggggggc gcgagtcggc cggcctgtac
     2221 caccgggcga agtacctgca ggtggcggcg ctggggctgg gcggcgtctc cctgctgctc
     2281 tggcccgggc cggtgcggtg ggagctgtgc gtgccgctcg gcgcggtcct cttgggcggg
     2341 ctggccagcg tgcagtgggc gtactacaag aagcacatgt aggtccggtg tcgttcgcgt
     2401 tcgcgacgga acaggccgga ttcagaatcg ccagaagctc ggagcttctg gctccggggc
     2461 gtccaggatc ggatcgctgc gccgcacgag cggatcgatc cagtcggcct tggctcgggc
     2521 ccactcaatc cactgctgct tctcctgtgt gagttcgccg tctcggtggg ctcggtcctc
     2581 gaccgcggcg atgaattcgc gcaaccggtt ggcgcgctgc agccggctcg cgtcgcggaa
     2641 cagggagcgc agctgacgac gttcttcgtt gcgcgcgcgc acctgcgctt cgtatcgggc
     2701 gcgcgcttcc tcgtaggttc gccggcggcg ctcttcttct tcctctttcg cgcacttcgc
     2761 ttcggccagg ccgacgatgg cggcgacgat tccgcccagg cgcgagtcga tgaagctgcg
     2821 ttccgtgtcg ttccacctcc gtgacggcca cgatcccacc gtgatcgtca gccgtccggt
     2881 ggggtgccag tcgaactgcg ggatgtgcgg atactctccc cgggcaccca cgcggaagga
     2941 gtcgtagtac cggtcgcgtg cccgcacctc cgcgagtgtc ggcgtgtggc tggttcgcgt
     3001 gacctgctcg acgatcgaga tcgtcaacgt ggtgccgccg ccgacgagca gggtctcgcc
     3061 cttctcctcg tccacccgcg ccgtgaagcc gctgggctcg agtgccttga gcagcgtgtc
     3121 cgccaaccgc agggcgcgat cgagcgcgtt cctcgtcacc tgcagatcga gcacctcctt
     3181 cgacgcactt ctcacgcccg ccgggtgatc ccagccgtcg cgccgcttca ggcgcgccgc
     3241 cgccgcccgt agcagcgggt gcgggtcgac gagttcgtcg ggcacgctga cgctcggctg
     3301 gctttcccgg gtctgctgga gagcgtcacg gacgcgcgcg tgcgtggccg cctcctgttc
     3361 ggacagcggt atcggtccgg cgagatcgcg cgcgccggcg ggtagggcgg gcagcggcac
     3421 cttgcgggtg gggcgaccgg ccttgacctt cgcccagtag ccccgcggcg gcaccggaat
     3481 gccgagcttc ttgcagatct tgaccagacc cacgtcggac aggccatagc ggggcgcgac
     3541 gaccgtgacc gcgtccgtcc agacctcttc gtacagtgcc tcccgggtga ctggggtgcc
     3601 ttggcgtctc atcgcatctc tcctagctcg agccggaggc cgtcattggc ggcggctccc
     3661 cggtagattg cagatttgct gttcagcaaa tattgtgttt tcgtcgaata ttcggtatgg
     3721 tgtcgatatt tactgcacag tgaacctagg cgacgaccat gctccaggcg caggcgagcg
     3781 aacgggaact gatcgatcag ttcctggcgg cacttcggtc gctgcccgaa gtgcgggccg
     3841 aactcgaagg ccccggcgcg cccgagctcg acgcccagct cgcgctggac gtcgccggca
     3901 agcccatcca cgcgctcgtc gagttgaaga gggccgtcta cccgcgcgac gtccggcagc
     3961 tcgtctggcg gatcagagac cttgcccgcc aacaacccgc cggcgagagc ggcggcgagc
     4021 cgctggcggt cctggtcgcc gagtccatct cgccgggggc taaggagctg cttcgagcgg
     4081 agcgggtcgg gtactacgac agcgggggca gcctctacct gccggcccgg ggagcctacc
     4141 tctacgtcga tcggccgccg cccaagtcgt tgtcccgctc catgcgctcg ctgttcacgg
     4201 gacggcgcgc gcaggtcctg cacggcctgt tagttcgcca ccaggactgg ttcggcgtga
     4261 aggacctcgc cgagcagacg ctggtctcgt cagcgacggc gtcccaggtg ctcaccgaga
     4321 tggagcgctt cgactggctg gagtcgcgcg ggcagggacc cagcaagcag aggcatctta
     4381 ggcagcccgc cgcgctgctc gatgcgtggg cgaagcagct gggctcgatc cgtgcgccgg
     4441 ccctgcgacg ctacttcgtc cccgcgatga agccagacgg cctgatggag cgcctcggcc
     4501 aggtgtgcga ggcgcgcggt gtcgagtact ccgtcagctt cgaggcggcc gcgcagcgct
     4561 acgcaccgtt tctgtcggca gtctcgcagg ttcgatgccg gctgctggca gggtcagggg
     4621 ccgatgccgc gatcgcggac ctgggcgcac gcgtcgttag cgagggcgcg aatctctccg
     4681 tcatcgaggc gaagtcgacg ggcgacctgc tgttccgcga gcgggtcggc ggcgtttggc
     4741 tggccagtcc aattcacgtc tacctcgatc tcctgcgagg cgagggccgg gccaaggaga
     4801 tggccgatca cctgcggaaa gagaggatcg gcttctgatg gccaagcccg cgacattgga
     4861 cggttacagc gaccagtaca cgctcgactg cgagcgggtg ctggtgacgc tgctgcgggg
     4921 actaggccca tggaaggact cggtctacct ggtcggcggc ctgacgccgc ggtatctcgt
     4981 ccccgcgcgc ccgccggtcg tgcctgcgca cgccggcacc ctggacgtcg acatcgtcat
     5041 cgatctgcag atcctggccg acaccgaggc gtaccacacg ctcgaagaga acctgaagc
//
LOCUS       DAOMFD010000144         5039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_6891_length_5039_cov_29.056581, whole genome shotgun sequence.
ACCESSION   DAOMFD010000144 DAOMFD010000000
VERSION     DAOMFD010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5039
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6891_length_5039_cov_29.056581"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..355)
                     /locus_tag="PL152_08280"
     CDS             complement(128..355)
                     /locus_tag="PL152_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49320.1"
                     /translation="MAKATTQLVVCLDNSGYEVSLERRKIYVSVPDGRAEKRRQIRVI
                     DESGEDYLYPQEYFVAIALPQPIKKAVLEAA"
     gene            complement(367..597)
                     /locus_tag="PL152_08285"
     CDS             complement(367..597)
                     /locus_tag="PL152_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49321.1"
                     /translation="MHFEFAGDLTEIETIATGSGIREIARLRKRYGRGRWRKRKGIAD
                     IRLSDGEVARAEIHWYEATGIGRREFKIKRFI"
     gene            complement(703..1503)
                     /locus_tag="PL152_08290"
     CDS             complement(703..1503)
                     /locus_tag="PL152_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009390813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HQR49322.1"
                     /translation="MGFLAGRRALIVGLATERSIAYGIASAMHREGAQLAFTYQNDRL
                     KDRVVAMAAEFGSTLTLPMDVATDAEIDQAFAQLRQHWDGLDILVHSVAFAPREQLAG
                     GYLDSVTREGFQVAHDISSYSLAALAKAARPMMLGRPGAIVTMSYLGAVRSIPSYNVM
                     GVAKASLEANVRFLAADLGPLGIRVNAISAGPIKTLAAAGVAGFRKMLSHVASYTPLR
                     RNVTIDEVGNAAAFLCSDLASGITGEVLYVDAGYSHVGMAFPEDEKGG"
     gene            complement(1508..3118)
                     /locus_tag="PL152_08295"
     CDS             complement(1508..3118)
                     /locus_tag="PL152_08295"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HQR49323.1"
                     /translation="MVVLGLALGGCSSTPSQGDYSSLPPTARHPASQPAAEQPGEVPP
                     DLSHFAHLPPDQYADLLDRIRAGYAIPDVQSSGVDREVEGYRSKPDYLDRTFRRGSRY
                     LYFIVSELERRGMPLELALLPVVESAFNPVAYSRSRAAGLWQFIPSTGKHYGLTQNWW
                     IDDRRDIRHSTMAALDYLQYLNRYFNGDWYLAIAAYNGGEGMVSAAVQRNLATGRPTD
                     FFSLDLRPETRDYVPKLLAIRRIVGDPSAYGLQFAPIANQPYFGVVDPGRQIDLGEAA
                     DLAGISRDDMFALNPEFNRMTTPPDGPHDLLLPVDAVEPFRMALLTEEGAAKVAVAVA
                     APPPTTTHEVKRGDTLSSIARRYGVSMAELSDANDLRDTVIHPGDTLQVPGASGATAT
                     LAAIAPAREDITAQLPEHQPSSSGKSGTRVHVVKSGDSLWSVARRYGVTVPALAAANG
                     LNSKSQLTAGARLEIPGRSSGGSSRSGEATRMTYHVKHGDTLSEIADRFNVSVRQLST
                     WNGLRQSTPLQTGQRLVVYADPRHVDGG"
     gene            complement(3181..3957)
                     /gene="gloB"
                     /locus_tag="PL152_08300"
     CDS             complement(3181..3957)
                     /gene="gloB"
                     /locus_tag="PL152_08300"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004416 - hydroxyacylglutathione hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0019243 - methylglyoxal catabolic process
                     to D-lactate via S-lactoyl-glutathione [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HQR49324.1"
                     /translation="MLDVSPVRAFSDNYIWLIRGPADPAVAVVVDPGDARPVNAALEQ
                     HGLRLHAVLVTHHHPDHVGGVAELAASHGARVYGPARERLPCEATTLDDGDTVRLAEP
                     GLEFVVMNVPGHTLGHIAYYGHGALFCGDTLFSGGCGRLFEGTPAQMLGSLDRIAALP
                     DDTRIYCAHEYTLSNLRFAASVEPGNADVIGALEATRVLRERDGISLPSTLGRERRIN
                     PFLRSREPTVRSAAEAHAGRAMAEPVEVFATVRSWKDGFR"
     gene            4086..4808
                     /locus_tag="PL152_08305"
     CDS             4086..4808
                     /locus_tag="PL152_08305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49325.1"
                     /translation="MAESSISEWLASAPGNALLQFETRVLQEALSDIVGFEMLQVGRW
                     GDDARLASYARTQRQWLVAPDAQGPGTVRASYDALPIATGAVEGVLLPHTLEYAPNPH
                     ELLREVERVLMGEGRVAICGFAPLGPWGLRHKVSRGRFLPYASRLLGDGRIGDWLRLL
                     GFEIELSQRFLFVTPWRGRRRGDPDGWFERRGPELFAPLAGAYVLKARKRVRALTPIR
                     PAWQRVPLVKVGVAEPTTRNAA"
     gene            4805..>5039
                     /locus_tag="PL152_08310"
     CDS             4805..>5039
                     /locus_tag="PL152_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HQR49326.1"
                     /translation="MTEVEIYTDGACKGNPGPGGWGAWLVSGGHHRELWGGEPHTTNN
                     RMELTAAIEALAALKRHCRVQLYTDSQYVRNGIT"
BASE COUNT          801 a         1689 c         1721 g          828 t
ORIGIN      
        1 cacggccaag gtcaagctga acgagaacgt gttcagcaac gacgaagggc agtagtccgc
       61 acgcagtacc gagcccggca gctgcaaagg ccgtgacgag cgaagcgcct cagcgtgcat
      121 cgggtcgtta ggccgcttcg agaaccgctt tctttatcgg ctgcggcaat gcaatggcga
      181 cgaagtattc ctgggggtag aggtaatctt ccccggactc gtcaattacc cgaatctgac
      241 gccgcttctc cgctcgacca tcgggcaccg atacgtatat ttttcgacgc tcgagtgaga
      301 cttcatagcc agaattatcc agacaaacta ccagctgtgt tgtcgcctta gccatggctt
      361 ctcgcctcag atgaagcgct tgatcttgaa ctctcgacgt ccgatacccg ttgcctcgta
      421 ccagtgtatc tcggcgcggg cgacttcgcc gtcggaaagc cgaatatctg cgattccttt
      481 ccgcttccgc caacgtccgc ggccatagcg cttccgtagc cgagcaatct cacgaattcc
      541 cgagccggtg gcgatcgttt caatttcggt cagatcgcct gcaaactcaa agtgcatggc
      601 gcatcgttcc agaccactcg tgatgggtca atcgaactcc gtcaaagcag catgacgcgt
      661 ggttgaccgg accctctcct cgggcctcgc gactccaagt ggtcagccgc ccttctcgtc
      721 ctccgggaac gccatgccca cgtggctgta tccggcatcc acgtacagca cctcgccggt
      781 gatgcccgac gcgaggtccg agcagaggaa cgccgcggca ttccccacct cgtcgatcgt
      841 cacgttgcgc cggagcggcg tgtaggacgc gacgtgcgag agcatcttgc ggaagcccgc
      901 cacgccggcg gctgcgagcg tcttgatcgg gccagccgaa atcgcattca cccgaatccc
      961 cagcgggccg aggtccgccg cgaggaatcg cacgttcgct tcgaggctcg ccttcgccac
     1021 gcccatgacg ttgtagctcg ggatcgagcg cacggcgcct agatagctca tcgtgacgat
     1081 cgcgccaggc cggcccagca tcatcggccg tgcggccttc gccagcgcgg cgaggctgta
     1141 gctcgagatg tcgtgggcga cctggaagcc ctcgcgcgtc acggaatcga ggtacccgcc
     1201 ggcgagctgc tcgcggggtg caaacgcgac cgagtgcacg aggatgtcga gtccgtccca
     1261 gtgctgccgc agctgcgcga acgcctggtc gatctcggcg tcggtcgcga cgtccatggg
     1321 cagcgtcagg gtggagccga actccgccgc catggcgacc acgcggtcct tcagccggtc
     1381 gttctggtag gtgaacgcga gttgcgcccc ctcgcgatgc atcgccgatg cgatgccata
     1441 ggcgatcgaa cgctcggtcg cgagcccgac gatcagggcc cgcctgcctg caaggaatcc
     1501 caccgggtca acctccgtct acgtgtcgcg ggtcggcgta tacgaccagg cgctggcccg
     1561 tctgcagcgg tgtggactgt cgcaggccgt tccaggtcga gagctggcgg accgacacgt
     1621 tgaagcggtc ggcgatttca gagagcgtgt cgccgtgctt cacgtgatag gtcatgcgcg
     1681 tggcttctcc cgacctggac gatccaccgc tcgaacggcc ggggatctcg aggcgcgcgc
     1741 ccgcggtcag ctggctcttc gaattcaggc cgttcgccgc ggcgagcgcc ggcaccgtga
     1801 ccccgtagcg ccgcgccacg ctccacagcg agtcgccgga cttcacgacg tgcacgcgcg
     1861 tccccgactt gccggaggaa ctcggctggt gctccggcag ctgggccgtg atgtcctcgc
     1921 gcgcaggcgc aatggccgcc agcgtcgcgg tcgcgcccga ggcgcccggc acctgcaacg
     1981 tgtcgcccgg gtggatgacg gtgtcgcgca ggtcgtttgc gtcgctcagc tcggccatcg
     2041 atacgccgta gcggcgcgcg atcgagctca acgtgtcgcc gcgcttcacc tcgtgggtgg
     2101 tcgtgggcgg aggggccgcc accgcgaccg ccaccttggc cgcaccttcc tcggtgagca
     2161 gcgccatgcg gaacggctcg acggcgtcga cgggcagcag caggtcgtgc ggcccgtcgg
     2221 gaggcgtcgt catgcggttg aactcggggt tcagcgcgaa catgtcgtca cgcgagatgc
     2281 ccgcgaggtc ggcggcttcg cccaggtcga tctggcggcc cgggtccacg acgccgaagt
     2341 acggctggtt tgcgatgggc gcgaactgca ggccgtaggc cgacgggtcg cccacgatgc
     2401 gccggatcgc cagcagcttc ggtacgtagt cgcgcgtttc cggccgcagg tccaggctga
     2461 agaagtcggt ggggcgcccg gtggccaggt tgcgctgcac ggccgcgctc accatgcctt
     2521 caccgccgtt gtacgcggcg atcgcgaggt accagtcacc gttgaaatag cggttgaggt
     2581 actgcaggta atcgagcgcc gccatcgtcg agtgccggat gtcgcggcga tcgtcgatcc
     2641 accagttctg cgtgagcccg tagtgcttgc ccgtgctcgg gatgaactgc cacaggccgg
     2701 ccgcgcggct gcgcgaatac gcgaccgggt tgaacgcgct ctcgaccacc ggcagcagcg
     2761 cgagctccag cggcatgccg cggcgttcga gttcggagac gatgaagtag aggtaacgcg
     2821 agccgcggcg gaacgtgcgg tcgaggtagt cgggcttcga gcggtagccc tcgacttcgc
     2881 gatccacgcc gctggattgc acgtcgggaa tggcgtatcc agcccggatg cggtcgagca
     2941 agtcggcgta ttggtcgggc ggcaagtgcg cgaagtggct gaggtcgggt ggcacctcac
     3001 ccggttgctc ggctgccggc tgggacgccg ggtgtcgcgc ggtgggcggc aggctcgaat
     3061 agtcgccctg cgacggcgtc gaactgcagc cgccgagcgc aaggcccaat acgaccaccg
     3121 gcgaaaatac tgcaatcggt gccaggcacc ggcgcaggaa tcgtgccagg catcggcgca
     3181 tcagcggaac ccgtccttcc aggaacgcac ggtggcgaag acctcgaccg gttccgccat
     3241 ggcccgtccc gcgtgcgcct cggcggcgct gcgtaccgtc ggctcgcggg agcgcaggaa
     3301 cggattgatg cgccgctcgc ggccgagcgt ggagggcagg ctgatgccgt cccgctcccg
     3361 gagcacgcgc gtcgcttcga gcgcgccgat cacatccgca ttgcccggct cgacgctggc
     3421 ggcgaatcgc aggttgctca gggtgtactc gtgcgcgcag tagatgcgcg tgtcgtcggg
     3481 cagggccgcg atgcggtcga gcgagccgag catctgcgcc ggcgtgccct cgaacagccg
     3541 cccgcacccg ccgctgaaca gcgtgtcgcc gcagaacagc gcgccgtgac cgtaataggc
     3601 aatgtgtccg agcgtgtgtc cgggcacgtt catcaccacg aattcgaggc cgggttctgc
     3661 aaggcggact gtgtctccgt cgtcgagggt ggtcgcctcg cagggcaggc gctcccgggc
     3721 cggtccgtag acccgcgccc cgtgactcgc ggcgagttcc gcgacgcccc cgacgtggtc
     3781 ggggtgatgg tgagtgacga ggaccgcatg gagcctgagt ccatgctgct cgagcgctgc
     3841 attgaccgga cgggcgtcac ccggatccac caccaccgcg actgccgggt ccgcaggacc
     3901 ccgtatcagc cagatgtagt tgtccgaaaa cgcccgcacc gggctcacat cgagcatgta
     3961 cgacccccgc catctgggcc atgagtccgg gcgcgcgtat tacacccgtt cacggggcaa
     4021 atatccagaa tatgtctcac gaatttcgaa cgtgatgggc acctttgccg ctatccttgc
     4081 cgccgatggc cgaatcttcc ataagcgagt ggctggcgtc agcgccgggg aatgcgctgc
     4141 tccagttcga gacgcgcgtg ctgcaggaag cgctctccga catcgtcggc ttcgagatgc
     4201 tgcaggtcgg ccgctggggc gacgacgcgc gcctcgcgag ctacgcgcgc acgcagcgcc
     4261 agtggctggt cgcgccggat gcgcaggggc ccggcaccgt tcgcgccagc tatgacgcgc
     4321 tgccgatcgc cacgggcgcg gtcgaaggcg tcctgctccc gcacacgctg gaatacgcgc
     4381 cgaacccgca cgaactgctg cgcgaggtcg agcgcgtgct gatgggcgag ggccgcgtgg
     4441 ccatctgcgg tttcgcgccg ctcggcccgt gggggctgcg tcacaaggta tcgcgcggcc
     4501 ggttcctgcc gtacgcgagc cgcctgctcg gcgacggtcg catcggcgac tggctgcgac
     4561 tgctcggctt cgagatcgaa ctgtcacagc gcttcctgtt cgtcacgccg tggcgcgggc
     4621 gtcgtcgcgg cgaccccgac ggctggttcg agcggcgtgg tccggaactc ttcgcgccgc
     4681 tcgccggcgc ttacgtgctc aaggcacgca agcgcgtgcg tgcgctgacg ccgatccggc
     4741 ccgcctggca gcgcgtgccg ctggtcaagg tcggcgtggc ggagcccacg acgagaaacg
     4801 cggcgtgacc gaggtcgaga tctacacgga cggggcctgc aagggcaacc cgggaccggg
     4861 cggctggggc gcgtggctcg tgtccggcgg gcatcaccgc gagctgtggg gcggggagcc
     4921 gcacacgacc aacaaccgca tggaactcac tgccgcgatc gaagcgctgg cggcgctcaa
     4981 gcgtcactgc cgcgtgcagc tctataccga ctcccagtac gtgcgcaacg gcatcaccg
//
LOCUS       DAOMFD010000145         4992 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7002_length_4992_cov_31.851934, whole genome shotgun sequence.
ACCESSION   DAOMFD010000145 DAOMFD010000000
VERSION     DAOMFD010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4992)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4992
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7002_length_4992_cov_31.851934"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PL152_08315"
     CDS             complement(<1..350)
                     /locus_tag="PL152_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49327.1"
                     /translation="MKSAARPIVALVATTLVPLTLTALAGCASTADTEGVEQEQVYQT
                     QGGVAVVDTFTTVATVTAVDAAKRRVTLTTPDGRSDTFKAAKGVDLAGFQVGAQMGVQ
                     LTDAVALQIRSDGTP"
     gene            478..1068
                     /locus_tag="PL152_08320"
     CDS             478..1068
                     /locus_tag="PL152_08320"
                     /inference="COORDINATES: protein motif:HMM:NF013412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HQR49328.1"
                     /translation="MYHDGNRALQDRFDSRRIADRLVEVLHRERFTVEDREFIESRPM
                     FFLATADADGRPDCSYKGGRPGFVRVIADDTLAFPSYDGNGMFKSVGNLVSNPAVGML
                     FMDFEQPRRLRVNGVASVDFADPLLAAFAGAQLVIRVRAEHIFPNCPRYIHRMALQEV
                     SAYAPAVGHEPPVPAWKEMEAFRDYLPRPGPTSSRK"
     gene            complement(1116..1691)
                     /locus_tag="PL152_08325"
     CDS             complement(1116..1691)
                     /locus_tag="PL152_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49329.1"
                     /translation="MRRFPILASVTVVASLLTAGAANAGAVSTIYRTGAYGLPWSADK
                     GAIQAHFPGGKWDQDEKGHERYCVTSRQNLLKLPSQYDSRELCFLVGKDGTLASATAV
                     LNPSLQSLLAIVNRCRTTFGDFDAVVRDPQAIQSRYSGQLWTRDAPYVVMVKSENDTD
                     GAPVRVTYTVADEGNLFTDGAAKVSNVLERK"
     gene            complement(1822..2439)
                     /locus_tag="PL152_08330"
     CDS             complement(1822..2439)
                     /locus_tag="PL152_08330"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HQR49330.1"
                     /translation="MRDADSPGLHAAILAAGPSTRFGSPKQLIRLAGTPVLHRAVSNA
                     ARAAGRSVTVVLGAGATDIAPVLRRSGASLVLNRGWEEGLASSVRAAVNAAPPGCEGL
                     MLLLADQLAVTSDDLLRLRVAWRRHPVLIAAALYGGAPALPAIFPGWAFSDLLGLRGD
                     ADPRVVIRRHSERVVRVPMPGAAIDVNTPEDLLEVPAGEQGRAEL"
     gene            2591..>4992
                     /gene="thrA"
                     /locus_tag="PL152_08335"
     CDS             2591..>4992
                     /gene="thrA"
                     /locus_tag="PL152_08335"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF007003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009067 - aspartate family amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HQR49331.1"
                     /translation="MESWKVHKFGGSSVADAACMKRVAEIVEADPASRKAVVLSACRG
                     VTDSLLGLVSMAERQDPALEDRIQDLRMRHVGIARELIGGGESYDEFIVQLDQDCQDI
                     AGILQAVRLVRSASTTLRDLIAGYGEIWSTRMFERYLKARGTGAGRVQWLDARKVLLV
                     EWGPLGPGIRWDQSRDNLHAQVEPGFTGTLVVTGFIATDTQGLQTTLGRNGSDFSASI
                     FGSLLDAGEIVIWTDVDGVLSADPRLVPEAQVIDALSYNEAMELAYFGAKVIHPQTMA
                     PAVHKRIPIWIKNTFAPDKAGTVIHDRTAPSPLVKGITTVEKIALVNLEGAGMIGVPG
                     TAHRLFGALREEGISVILISQGSSEHSICFAVPEVEADRAERVVRRAFDPELREGQIQ
                     SVEVNRNCSILAVVGDGMAGAHGVAAQVFSALGKASVNVRAIAQGASERNISVVIDGR
                     GSARALRAVHSSFYLSAHTVSIGLIGPGLVGGALLDQIASQADRLRRDFKLDLRVRGI
                     AGTKSMRLAQTQVDLARWRDEYAAGEGPVDLERFADHVHADQIPHAVIIDCSASAEVA
                     TRYAHWLSEGIHVVTPNKKANSAAYGYYEQLREARRSAGAHYLYEATVGAGLPVIQTL
                     RDLRETGDEIRRIEGMFSGTLAYLFNTWDGSQSFSSIVRQAKALGYTEPDPRDDLSGM
                     DVARKLIILGREMGLRLELADVVVESLVPAALAGGGIEEFLERLPEFDEPMLARLRAA
                     HAHGHVLRYVGSLGADGQAKVGLVELPVSHPFANIALTDNIVRFQTARYDKNPLIVQG
                     PGA"
BASE COUNT          775 a         1685 c         1753 g          779 t
ORIGIN      
        1 ggcgtgccat cgctccggat ctggagcgcg acggcgtcgg tgagctgaac gcccatctgc
       61 gcgccgacct ggaaacccgc gaggtcgacg cctttcgcgg ccttgaacgt gtcggacctc
      121 ccgtcgggtg tcgtcagggt caccctgcgc ttggccgcgt cgactgccgt caccgtggcc
      181 acggtggtga acgtatcgac gaccgcgacc ccgccctggg tctggtacac ctgctcctgc
      241 tcgacgcctt ccgtgtccgc ggtgctcgcg cagcccgcca atgcggtgag cgtcagggga
      301 accagcgtgg tggcgacgag cgcgacgatc gggcgggcag cagacttcat gcttcgaacc
      361 tcaggttggg gtgtttggac ccggcaatga tagcggggtg gcgctggccg gcgatgatct
      421 gcacgacggg cgtacactgc cggccggcgc ccgtttcccg tgtcgcggag gcattccatg
      481 taccacgatg gcaatcgcgc cctgcaggac cgcttcgatt cccgccgcat cgccgaccgc
      541 ctcgtcgagg tgctgcaccg tgagcggttc acggtcgagg accgtgaatt catcgagagt
      601 cgcccgatgt tcttcctcgc gacggccgac gccgatggca ggccggactg ctcctacaag
      661 ggcggccggc ccgggttcgt ccgggtgatc gccgacgaca cgctggcgtt cccgagctac
      721 gacggcaacg ggatgttcaa gagcgttggt aacctggtca gtaaccccgc cgtcgggatg
      781 ctcttcatgg acttcgagca gcccaggcgc ctgcgggtga acggcgtggc gtcggtggac
      841 ttcgcggatc cgctgctcgc ggcgttcgcg ggcgcacagc tggtcatccg cgtgcgtgcc
      901 gagcacatat ttcccaattg cccgcgctac atccaccgga tggcgttgca ggaagtttcc
      961 gcatacgcgc ccgccgtcgg gcacgagccg cccgtgccgg cgtggaagga gatggaggcg
     1021 ttccgcgact acctgccgcg gccggggccg acgtccagcc ggaaatgaaa aagccggcga
     1081 ggctttgcgg cccgccggct gtgagttgga aagggctact tgcgctcgag gacgttcgag
     1141 accttggcgg cgccgtccgt gaacaggttg ccctcgtcgg cgacggtgta ggtcacgcgc
     1201 accggcgcac cgtccgtgtc gttctcgctc ttgaccatga cgacgtacgg ggcatcccgg
     1261 gtccagagct ggccgctgta gcgcgactgg atcgcctgcg ggtcgcgcac cacggcgtcg
     1321 aagtcgccga aggtcgtgcg gcagcggttg acgatggcga gcagcgactg cagcgacggg
     1381 ttgagcacgg ccgtggcgct ggccagggtg ccgtccttgc cgaccaggaa gcagagttcg
     1441 cggctgtcgt actgcgaggg aagcttcagc aggttctgcc ggctcgtgac gcaatagcgc
     1501 tcgtggccct tctcatcctg gtcccacttg ccgcccggga aatgggcctg gatggcgccc
     1561 ttgtcggcgc tccagggcag cccgtaggcg ccggtgcggt agatggtgct gaccgcccct
     1621 gcattggcag ccccggcggt caaaagggac gcgacgacag tgacgcttgc caggatcggg
     1681 aatcgacgca tacccatggt ctccagaggt cggtggtggc gcaagtgtag gcaaggaggg
     1741 cgctggcgca agattgcgcc ttgcggaatc acgacgcgct tctcaatcgg gcccgattcg
     1801 cgccggtaat tatgaggccg ttcagagttc tgcgcgaccc tgctcgccgg ccgggacctc
     1861 gaggaggtcc tccggcgtat tcacgtcgat cgcggcgccc ggcatcggga cccgcacgac
     1921 ccgctcggag tggcggcgaa tgacgaccct ggggtctgcg tcgccacgca ggccgagcaa
     1981 gtcggagaag gcccagcccg ggaagatggc gggcagcgct ggcgcgccgc cgtaaagggc
     2041 ggcggcgatc agcaccgggt ggcggcgcca tgcgacgcgc agccgcagca ggtcgtcgga
     2101 ggtcaccgca agctggtcgg caagcagcag catcaggccc tcgcatcccg gtggcgccgc
     2161 attgaccgcg gcccgcactg agctggcgag tccttcctcc cagcctcggt tcagcaccag
     2221 cgaagccccc gaccgacgca ggaccggagc aatgtcggtg gccccggccc ctagcaccac
     2281 ggtgaccgac cggcccgccg cccgggccgc attcgagacc gcccggtgca gaaccggcgt
     2341 cccggccagg cggatgagtt gtttcgggct cccgaagcgg gtcgaagggc ccgccgccag
     2401 gatggctgcg tgcagtccgg gggagtccgc gtctcgcatg gagtgtagtt tgcctgccgc
     2461 gctgcaagaa gatacagtcc cttgcaccga aaccgaggtg tcccccgtca gaccatgccc
     2521 ggcctgaccc gtcgcctgcc ggcgatcggt cggcgtgccc ccgcgggcgg ctgcaacgga
     2581 gccctgcatc gtggaatcct ggaaagtaca caagttcggc gggtcgagcg tcgcggacgc
     2641 cgcctgcatg aagcgcgttg ccgagatcgt cgaggccgat cctgcctcga ggaaggcggt
     2701 cgtcctctcg gcctgccgtg gcgtcacgga cagcctcctg ggcctggttt cgatggctga
     2761 gcgccaggac ccggcgctcg aggatcgcat ccaggacctg cgcatgcgcc acgtgggcat
     2821 cgcccgcgag ctcatcggcg gcggcgagtc ctacgatgaa ttcatcgtcc agctcgacca
     2881 ggactgccag gacatcgccg ggatcctcca ggcggtgcgg ctggtccgct cggcctcgac
     2941 gacgctcagg gacctgatcg ccggctacgg tgaaatctgg tccactcgca tgttcgagcg
     3001 ctacctgaag gctcggggca ccggcgctgg ccgcgtccag tggctcgacg cgcgcaaggt
     3061 gctgctggtc gagtggggac cgctcgggcc cggcatccgc tgggaccagt cgcgcgacaa
     3121 cctgcatgcg caggtcgagc cgggcttcac cggcaccctg gtggtcaccg gcttcattgc
     3181 caccgacacc cagggcctgc agacgaccct cggccgcaac ggcagcgact tttccgcctc
     3241 gatcttcggc tcgctgctcg acgccggcga gatcgtcatc tggaccgacg tggacggcgt
     3301 gctgagcgcc gacccgcgcc tcgtgcccga ggcccaggtg atcgatgcgc tctcctacaa
     3361 cgaagccatg gagctcgcgt atttcggagc caaggtcatc cacccgcaga ccatggctcc
     3421 ggcggtccac aagcgcatcc cgatctggat caagaacacg ttcgcgccgg acaaggcggg
     3481 aacggtcatc cacgaccgca ctgcgccgag cccgctggtc aagggcatca cgaccgtcga
     3541 gaagatcgcg ctcgtgaacc tcgaaggcgc cgggatgatc ggcgtcccgg ggacggcgca
     3601 ccgcctgttc ggcgcactgc gcgaggaagg catctccgtg atcctgatct cgcaaggcag
     3661 ttcggagcac tcgatctgct tcgccgtgcc ggaggtcgag gccgaccgcg ccgagcgtgt
     3721 cgtgcgccgg gctttcgatc cggagttgcg cgaagggcag atccagagcg tcgaggtcaa
     3781 tcgcaactgc agcatcctgg ccgtcgtggg cgacgggatg gccggtgcgc acggcgtggc
     3841 ggcgcaggtc ttcagcgcgc tcggcaaggc cagcgtcaac gtccgcgcga tcgcccaggg
     3901 cgcatcggaa cgcaacatct cggtcgtcat cgacgggcgc ggcagcgcgc gggcgctgcg
     3961 ggccgtgcat tcgagcttct acctgtccgc gcacacggtg tcgatcggcc tcatcgggcc
     4021 ggggctcgtg ggcggtgcgc tgctcgacca gatcgcctcg caggcggacc gcctgcggcg
     4081 cgacttcaag ctggacctgc gcgtgcgcgg catcgccggc acgaagagca tgcgcctggc
     4141 gcaaacgcag gtggatctcg cacgctggcg cgacgagtac gcggcgggcg aggggccggt
     4201 ggacctcgag cgcttcgccg atcacgtgca cgcggaccag attccgcacg ccgtcatcat
     4261 cgactgcagc gcgagcgccg aagtcgcgac gcgctacgcc cactggctct ccgaaggcat
     4321 ccacgtggtg acgccgaaca agaaggcgaa cagcgcggcc tacggctact acgagcagtt
     4381 gcgggaggcg cggcggtctg ccggtgccca ctatctgtac gaggcgacgg tgggcgccgg
     4441 gctgccggtc atccagacgc tacgcgacct gcgcgagacg ggcgacgaga tccggcgcat
     4501 cgagggcatg ttctcgggca cgctcgccta cctgttcaac acctgggacg gcagccagtc
     4561 gttctcgtcg atcgtcaggc aggccaaggc cctcgggtac accgagcccg atccgcggga
     4621 tgacctgtcg ggcatggacg tcgcgcgcaa gctcatcatc ctcgggcgcg agatggggct
     4681 gcgactcgaa ctcgcggacg tcgtcgtcga aagcctcgtg ccggcggcac ttgcgggcgg
     4741 cggcatcgag gaatttctcg agcggctgcc ggagttcgac gagccgatgc tggcacggtt
     4801 gcgcgcggca cacgcgcacg ggcacgtgct gcgctacgtc ggttccctcg gcgcggacgg
     4861 ccaggcgaag gtgggcctcg tggaactgcc ggtctcccat ccgttcgcga acatcgcgct
     4921 cacggacaac atcgtgcgct tccagacggc gcgctacgac aagaacccgc tgatcgtgca
     4981 gggcccgggc gc
//
LOCUS       DAOMFD010000146         4922 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7170_length_4922_cov_30.063900, whole genome shotgun sequence.
ACCESSION   DAOMFD010000146 DAOMFD010000000
VERSION     DAOMFD010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4922)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4922
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7170_length_4922_cov_30.063900"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..746)
                     /locus_tag="PL152_08340"
     CDS             complement(<1..746)
                     /locus_tag="PL152_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49332.1"
                     /translation="MDDRNAGLEAVVAELQQSVRLLQQRVEILEAGVASSPSAPRTRE
                     EGAPQAAATKAARRDPYDPIAILTLVGRLFLVLAGGFFLRAMTESGVLVPPLGLSVGF
                     AYAMVWLYFADRAGGRGEVPGAVFHALGAALIAYPLLVEAATKFKVLGGSSSAMAVTV
                     LTAALLFVAAHRRLRAVGWITVLGALPTSAVLLSQTGVIAPFALFLIVFGVATLWFGY
                     TLDWWGMRWPAALAADVVVIGVTLRVLAPE"
     gene            complement(914..1927)
                     /locus_tag="PL152_08345"
     CDS             complement(914..1927)
                     /locus_tag="PL152_08345"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HQR49333.1"
                     /translation="MSPQSLANAPVALMPVLIFLAALVYLDTFKLVSRRFVALAMAAG
                     ALAAGASYYLNGVLLDLLQVEFVTYSRYFSPLLEESLKAAILVFLIRTRRIGLLVDAA
                     IAGFAVGSGFALVENVYYLASRPDTHVAVEIIRGFGTAIMHGGATSIFAIVSVALAEK
                     RPDATVSAFLPGLLAAAALHSAFNHLLARPVLATFGILVALPPLMHYVFRRSEASLRD
                     WLEADLDSDIELLELINSGEFSSSHAGTYLHSLQENMRGEVVADMLCYMRLHVELALR
                     AKGVLIMRENGIDAPLDDETREKLAEMQYLERSIGRAGQLAVRPVLKMTSKDLWQIYM
                     LGQ"
     gene            complement(1955..>2464)
                     /locus_tag="PL152_08350"
     CDS             complement(1955..>2464)
                     /locus_tag="PL152_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49334.1"
                     /translation="LLGEFDLGFQLPIGHSSIWTRNYAGVSSGSSDNPLTNAYFGGFG
                     NNYVDHGDPKRYRDIFSMPGYEIDALGGKVFFKSLLEWDLPPLRFEHLGTPGFYVSWA
                     RPAIFATALLLDPQESRSRSDAYDAGVQVDFQLQVMQGLPMMLSFGYAVGFGDDRDKG
                     EFMVSLKIL"
     assembly_gap    2465..2564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2565..4765)
                     /locus_tag="PL152_08355"
     CDS             complement(<2565..4765)
                     /locus_tag="PL152_08355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49335.1"
                     /translation="MDRRDGETRPRHRLQGGRFLPAALVLLVVQSAVRADGINFLETE
                     HLRMVDFAPAEDYLVPYATQCFLNALASQKKIFGYVPDGKASVLLQDFSDRGNATTVL
                     AAPRDRVFVTIAPMVLNFETFSPGERMFTIANHEAVHLVASDMANATDMHWRRIFMGK
                     VAPVAEHPESILYYYLTVPRASSPRWYFEGSAVFMETWLGGGLGRAQGGYDEMVFRAM
                     VRDDALFYDPLALVSKGTEVDFQVGANAYLYGTRFMSYLGLVYGPEKLVEWWSRGPDS
                     ERYYADEFQRVYGMPLATAWQDWVRWERGFQQKNLESVRQHPITPYHDVAPEGLGAIS
                     RAYLSADGTKLFAGVRYPGRVPHLVEISIADGRVQELQEILGAVSVRVTSLAYDPATE
                     TLFYTTDNLNYRNLMAFDLKTRTSRLLLKGARIGDLVFNPVDRSIWGLRTNNGFVILV
                     RIPYPYKDWQSVHVFPFSEVPFDLDISPDGALLSMSVAGPDEKNPRMQVMQVRVMPTA
                     ALLAGDATPTRKFMLGSAVPEGFAFSRDGKYLLGSSFYTGVSNIYRYELATGQLDALS
                     NAEIGFFRPIELDDSRLVVFHYTARGFVPATIEPKVTEDLSAITFLGEQVATKWPVVQ
                     SWVAGSPDSIDYRAQVQREGEYSAVREIGLESLYPVIQGYKDSVSYGMNAQFSDPIGF
                     DTASLTAGYSPDDSLPGKQRGHAQATFHHSFWTATLKWNAADFYDLFGPTKV"
BASE COUNT          847 a         1624 c         1621 g          730 t
ORIGIN      
        1 tgctcgggtg cgaggacccg cagcgtcacg ccgatcacca ccacgtcggc cgccagcgcg
       61 gcaggccagc gcatgcccca ccagtcgagc gtgtaaccga accacagtgt cgccacgccg
      121 aacacgatca ggaacaatgc gaagggggcg atcacgccgg tctgcgacag gagcacggcg
      181 ctggttggca gcgcaccgag caccgtgatc cagcccacgg cgcgcagtcg ccggtgtgcg
      241 gccacgaaca gcaaggcggc cgtcaacacg gtgaccgcca tcgcgctcga gcttccgccc
      301 agcaccttga acttcgtcgc ggcttccacc agcagcggat aggcgatcag tgcggcgccc
      361 agcgcatgga agaccgcacc cgggacctcg ccgcgcccgc ccgcgcggtc ggcgaagtac
      421 aaccagacca tcgcgtaggc gaatccgacc gacagcccga gtggcggaac gagcacgccc
      481 gactcggtca tcgcgcgcag gaagaatccg ccggccagca cgaggaacag ccggcccacg
      541 agggtgagga tcgcgatcgg atcgtatgga tcccgccggg cggccttggt ggccgctgcc
      601 tgcggcgctc cttcctcacg cgttcgcggc gcggaagggc tgctggcgac gcccgcctcc
      661 aggatttcga cgcgctgctg cagcaaccgg acggactgct gaagctccgc cacgacggcc
      721 tcgagacctg cgttgcgatc gtccacggtg cccccctggt gccgcgtcgt tgtcggtctg
      781 cggtcacgcg ccgccgcgcg ggactgctgc cgcatcacta cagcctggcg cgcaaaaagc
      841 catgagggat tgccgaaact cgacgcagcg cggggaggcc gcagtacgtc acgctgcccg
      901 cgggcgcggc gagtcactgc ccgagcatgt agatctgcca gaggtccttg ctggtcatct
      961 tgagcacagg ccggacggcg agctgcccgg cccggccgat gctgcgctcc aggtactgca
     1021 tctcggcgag cttctcgcgc gtctcatcgt cgagcggcgc gtcgatgccg ttctcgcgca
     1081 tgatgagcac gcccttcgcg cgcaacgcca gctccacgtg caggcgcatg tagcagagca
     1141 tgtcggcgac cacttcgccg cgcatgttct cctgcagcga gtgcaggtac gtcccggcgt
     1201 gcgaactcga gaactcgccg gagttgatga gctcgagcaa ctcgatgtcg gagtccaggt
     1261 cggcctcgag ccagtcgcgg agcgacgctt cgctgcggcg gaacacgtag tgcatgagcg
     1321 gcggcagggc gaccaggatc ccgaaggtcg ccaggacggg ccgtgcgagc aggtgattga
     1381 atgccgagtg cagcgccgcg gcggcgagca gcccgggcag gaacgccgac accgtcgcat
     1441 ccggccgctt ctcggccagc gcgaccgaca cgatcgcgaa gatggacgtg gcgccgccat
     1501 gcatgatcgc cgtgccgaag ccgcggatga tctcgaccgc cacatgcgtg tccgggcgcg
     1561 atgccaggta atagacgttc tcgacgagcg cgaatcccga accgaccgcg aagcctgcga
     1621 tcgcggcgtc caccagcagg ccgatgcggc gcgtgcggat caggaacacg agtatggccg
     1681 ccttgagcga ttcctcgagc agcggcgaga agtaacgcga gtaggtgacg aactcgacct
     1741 gcagcaggtc gagcagcacc ccgttcaggt agtagctggc acccgctgcc agcgcccccg
     1801 cggccatcgc gagcgccacg aaacgcctgc tgaccagctt gaaggtgtcg agatagacga
     1861 gcgcggccag gaagatcagc accggcatca gcgcgaccgg cgcattggcc agggactgcg
     1921 ggctcatccg gtcgcgccct gcggatacgg cggcctacag gatcttgagg gagaccatga
     1981 actcgccctt gtcacgatcg tcaccgaagc ccaccgcgta cccgaaggac agcatcatcg
     2041 gcagcccttg catgacctgc aactggaaat cgacctgcac cccggcgtcg taggcgtccg
     2101 accgggagcg gctctcctgc gggtcgagca gcagcgcggt ggcaaagatc gccggacgcg
     2161 cccagctcac gtagaaaccc ggcgtgccca ggtgctcgaa tcgcagcggc ggaaggtccc
     2221 actcgagcag cgacttgaag aagaccttgc cgccgagcgc atcgatctcg tagcccggca
     2281 tgctgaagat gtcgcgataa cgctttggat caccgtggtc cacgtagttg ttgccgaagc
     2341 cgccgaagta cgcgttcgtc agcggattgt cgctgcttcc ggacgacacc ccggcgtagt
     2401 tgcgcgtcca gatcgacgag tggccgatcg gcagctggaa ccccaggtcg aactcgccga
     2461 gcagnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnaccttg gtcggcccga
     2581 acaggtcata gaagtccgcg gcgttccact tgagcgtcgc ggtccagaac gagtggtgga
     2641 acgtcgcctg cgcatgcccc cgttgcttgc ccggcaacga gtcgtccggg ctgtagcccg
     2701 cggtgagcga cgccgtgtcg aagccgatcg gatcgctgaa ctgcgcgttc atgccgtagg
     2761 agaccgagtc cttgtaaccc tggatcacgg gatacaggga ctcgaggccg atctcgcgga
     2821 cggccgagta ttcgccctcc cgctgcacct gtgcccggta atcgatgctg tccggcgagc
     2881 ccgcgaccca gctctgcacg accggccatt tcgtcgcgac ctgctcgccg aggaaggtga
     2941 ttgcgctcag gtcctccgta accttcggct cgatggtcgc cgggacgaag ccgcgagcgg
     3001 tgtagtggaa gacgaccagc cgcgagtcgt cgagttcgat gggccggaag aatccgatct
     3061 ccgcgttcga cagcgcgtcg agctgcccgg tcgccagctc gtagcgatag atgttcgata
     3121 ccccggtgta gaaggagctg ccgagcaggt acttcccgtc gcgggagaat gcgaacccct
     3181 cgggaaccgc gctgccgagc atgaacttgc gggtcggcgt cgcatcaccg gcgagcagtg
     3241 ccgccgtcgg catcacgcgg acctgcatga cctgcatgcg cgggttcttc tcgtccggcc
     3301 ccgccaccga catcgacagc agcgctccgt ccggcgaaat gtcgaggtcg aacggcacct
     3361 cgctgaaggg gaacacgtgg acgctctgcc aatccttgta cggatacgga atgcgcacca
     3421 ggatcacgaa tccgttgttc gtcctcaggc cccagatcga gcggtcgacg gggttgaaga
     3481 cgaggtcgcc gatgcgcgcg cccttgagca gcaggcgtga ggtgcgcgtc ttcaggtcga
     3541 acgccatcag gttgcggtag ttgagattgt cggtcgtgta gaagagcgtc tccgtcgccg
     3601 ggtcgtaggc gagcgacgtg acccgaaccg agacggcgcc gaggatctcc tgcagttcct
     3661 gcacgcggcc gtcggcgatc gagatctcga cgaggtgcgg gacgcgcccg ggatagcgca
     3721 ctccggcaaa gagcttggta ccgtcggcgc tcaggtaggc gcgcgagatc gctcccagcc
     3781 cttccggcgc gacgtcgtga tacggggtga tcgggtgctg gcgcaccgat tccaggttct
     3841 tctgctggaa gccgcgctcc caccgcaccc agtcctgcca cgcggtcgcg agcggcatgc
     3901 cgtacacgcg ctggaactcg tcggcgtagt agcgctccga gtccggtccg cggctccacc
     3961 actcgaccag cttctccgga ccgtacacga gcccgaggta gctcatgaaa cgcgtcccgt
     4021 agaggtacgc gttggcaccg acctgaaagt cgacctcggt gcctttcgag acgagcgcca
     4081 gggggtcgta gaacagcgcg tcgtcgcgga ccatcgcccg gaacaccatc tcgtcgtagc
     4141 caccctgcgc gcggccgagc ccgccgccga gccacgtttc catgaacacg gcgctgcctt
     4201 cgaagtacca gcgcggcgag gaggctcgcg gcacggtgag gtagtaatag aggatcgact
     4261 ccgggtgctc cgccaccggg gccaccttgc ccatgaagat gcgccgccag tgcatgtcgg
     4321 tggcattcgc catgtccgag gccacgaggt gcaccgcctc gtgattggcg atggtgaaca
     4381 tgcgctcgcc cgggctgaag gtctcgaagt tcagcaccat cggcgcgatg gtgacgaaga
     4441 cccggtcccg gggcgccgcc agcacggtgg tcgcattgcc gcggtcggaa aagtcctgca
     4501 ggaggaccga cgccttgccg tccgggacgt agccgaagat cttcttctgg gaggcgagcg
     4561 cgttcaggaa gcactgcgtc gcgtacggca cgaggtagtc ctcggccggc gcgaagtcga
     4621 ccatccgcag gtgctcggtt tccaggaagt tgatgccgtc cgcgcggacc gcggactgga
     4681 cgacgagcag cacgagcgcg gccggaagga accgccctcc ctgcaggcga tgacgcgggc
     4741 gggtctcccc gtcgcgacgg tccacgtgtc ttgctgccgg tctttatctg gcgccgggtg
     4801 caggagcggc ccgccccgcc gggacgagcc actccctccg caagcgcgac cccgcccaag
     4861 ggcggggcct catccggcta ctgatgcgcg tcagcagctt tggctgcgtc ggccgccttg
     4921 gc
//
LOCUS       DAOMFD010000147         4875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7293_length_4875_cov_34.776349, whole genome shotgun sequence.
ACCESSION   DAOMFD010000147 DAOMFD010000000
VERSION     DAOMFD010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7293_length_4875_cov_34.776349"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..1538)
                     /locus_tag="PL152_08360"
     CDS             complement(21..1538)
                     /locus_tag="PL152_08360"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HQR49336.1"
                     /translation="MDFDCAEKTKNKRAPALVLITLCGAVAAVVLGGCAKPGDAVTEA
                     AKTDAAAGVAAPGIEEIKAIAEEAYIYGFPMIAAYKAMYEFNVDTTSPQYKAPFNQIW
                     NDSKTFTPKDTAIVTPNSDTPYSMLQADLRAEPLVLCVPAVEKKRYYSVQLTDLYSFN
                     YGYIGSRATGNDPGCYMIAGPDWSGDTPAGIAKVFQSETQFSFIIYRTQLFNPADIDN
                     VKKIQAGYKVQPLSAFLGQPAPAAAPAIDFPKFVGEDPFKTEFPAFLDFLLQFAPAIG
                     PAAADAPLRAKFASIGIGPGKTFDFKDLSLEQKAAFGLAIKQAFEKIDKASSSIGKSI
                     NGWHVGSAFGDRAFYKGDWLLRAAAAKAGIYGNNAEEAMYPMTRVDSTGQTIDTSKHD
                     YTLTFAKGELPPVNAFWSVTMYDGKSQLLIENPINRYLINSPMLPGMKKNPDGSLTIY
                     IQKDSPGKDKEANWLPAPNDTVYLVMRLYWPKTEAPSILPAGEGTWSPPAVVQAR"
     gene            complement(1643..3100)
                     /locus_tag="PL152_08365"
     CDS             complement(1643..3100)
                     /locus_tag="PL152_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux transporter outer membrane subunit"
                     /protein_id="HQR49337.1"
                     /translation="MPGISRKSLAAALATLLALAACSQAPVLEVPSVPVGASYKEASG
                     GERAEAARPLQPDAWWTLYGDPELDALEQQLIANSPDLAAALARYKEANAAYQAIRSN
                     LFPTLSTALNAQRDRQSESKPLRVLGPNSPNEYDSVALGFGLGYEFDLWGRVSNEVAA
                     GRALDEAARADLDAARLSLQALLAESYFILRGLDRQIELLTETEAAFRRALELVQSRH
                     VGGIASGLDVSRAEAQLEAARSLLKQYVGQRAVVEHAIASQVGVPAPDFSIAPRTDAI
                     PLPDVPVDVPSTLLQRRPDILAAERRVEAANASVGVAKAAFFPQLRLDATYGYQSTEF
                     ANLVTAPNVFWAIGPTLLMDLFDGGRRQADVAHAEAVLDETGANYRSVVLGAFQEVED
                     SLALLDNYRQAAVSEAAAVAATQRSVDLSTNRYRAGAASYLEVTVSQASALQTRRDAQ
                     DLTTRQLRASVQLIRALGGGWASGSGCREQSANPC"
     gene            complement(3087..4292)
                     /locus_tag="PL152_08370"
     CDS             complement(3087..4292)
                     /locus_tag="PL152_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR49338.1"
                     /translation="MPADTPSPASPARTVRRIVTIAAFAAVVVVVYGAATRIVDAQRL
                     RDRTADAAVPTVTIVTPGPAAAGAPLELPGRVEAFAQAPIYARVSGYLKAWYADIGTK
                     VRAGQLLAEIETPDLDQQLLQAQAELANARANAALAETTAKRWQSLLASDSVSQQEVE
                     EKTADLAARQAEVRALQANVDRYQALKQFARIVAPFDGVVTRRETDVGALINVGSAQG
                     PELFVVADLGKLRVYVNVPQNYASQVHPGTEATLTVPERPGRQYAATVQSTSNAIDPA
                     SGSMLVQLTVANASSELLPGGFARVRFDIPSAAGALSIPASALIFDKDGLQVATLGTG
                     DKVVLKPVTLSRDMGATVELSAGITPTDKVIENPPDGVANGDAVRVAAPAEKPGAAGA
                     KGQDGHAGH"
     gene            complement(4282..>4875)
                     /locus_tag="PL152_08375"
     CDS             complement(4282..>4875)
                     /locus_tag="PL152_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49339.1"
                     /translation="PKGSSVELVGQVRTMDHAFTGLLFGLAGAIVLIYLLIVVNFQSW
                     TDPFVIITALPAALAGIVWMLFATHTTLSVPALTGAIMCMGIATANSVLVIAFARERL
                     DAGADPAQAALEAGFVRFRPVLMTALAMIIGMLPMALGLGEGGEQNAPLGRAVIGGLC
                     FATVATLVFVPVVFSLVHGRRRALAGQAAMPGELHAS"
BASE COUNT          717 a         1658 c         1715 g          785 t
ORIGIN      
        1 ccgtcgcgct ggcccgcaca ctacctggcc tggacgaccg ccggcggcga ccacgtgccc
       61 tcgccggccg gcaggatcga cggtgcctcg gtcttcggcc agtacagccg catcacgagg
      121 tacaccgtgt cgttcggcgc ggggagccag ttggcttcct tgtccttgcc gggcgagtcc
      181 ttctggatgt agatcgtcag cgagccgtcc ggattctttt tcattccggg cagcattggc
      241 gagttgatca ggtagcggtt gatcgggttc tcgatcagca gctggctctt gccgtcgtac
      301 atcgtcaccg accagaacgc gttgaccggc ggcagctcgc ccttggcgaa ggtcagcgtg
      361 tagtcgtgct tgctggtgtc gatcgtctgg ccggtcgagt cgacgcgcgt catcgggtac
      421 atggcctctt cggcgttgtt gccgtagatg ccggccttcg cggcggcggc gcgcagcagc
      481 cagtcgccct tgtagaacgc gcggtcaccg aacgccgagc cgacgtgcca gccgttgatg
      541 ctcttgccga tcgagctcga ggccttgtcg atcttctcga acgcctgctt gatcgcgagg
      601 ccgaacgcgg ccttctgctc gagcgagagg tccttgaagt cgaaggtctt gcccggaccg
      661 atgccgatgc tcgcgaactt cgcgcgcagc ggcgcgtcgg cggcggccgg gccgatggcc
      721 ggcgcgaact gcagcaggaa gtcgaggaaa gccgggaatt cggtcttgaa tggatcctcg
      781 ccgacgaact tcgggaaatc gatcgcaggc gcggccgccg gtgccggctg gccgaggaac
      841 gccgacagtg gctgcacctt gtagccggcc tggatcttct tgacgttgtc gatgtcggcc
      901 gggttgaaca gctgcgtccg gtagatgatg aagctgaact gggtctcgga ctggaacacc
      961 ttggcgatgc ccgcgggcgt gtcgcccgac cagtccggcc cggcgatcat gtagcagccc
     1021 gggtcgttgc cggtggcgcg gctgccgatg tacccgtagt tgaacgagta gaggtcggtg
     1081 agctgcaccg agtagtagcg cttcttctcg acggccggga cgcagagcac cagcggctcg
     1141 gcgcgcaggt ccgcctgcag catcgagtac ggggtgtccg agttcggcgt gacgatcgcg
     1201 gtgtccttcg gcgtgaaggt cttcgagtcg ttccagatct ggttgaacgg ggccttgtac
     1261 tgcggcgagg tcgtgtcgac gttgaattcg tacatcgcct tgtacgccgc gatcatcgga
     1321 aagccgtaga tgtaggcttc ctcagcgatc gccttgatct cctcgatgcc gggggcggcg
     1381 acgccggcgg cggcgtcggt ctttgccgcc tcggtgacgg cgtcgcccgg cttggcgcag
     1441 ccgcccagca cgactgcggc gacggccccg cacaatgtga tcaggaccag ggcaggtgcc
     1501 cgcttgttct tcgtcttctc ggcgcaatcg aaatccacgg gggttctcct acgcgttcac
     1561 gcggcacgaa gcgcgcattg taaagggaga cgacatctgc gccaccatcg gccatgcggc
     1621 gcggatgccg gcccggaccg actcagcacg ggttggccga ttgctcccgg cagccggagc
     1681 cagacgccca cccacccccg agcgctctga tcagctgcac acttgcgcgg agctggcggg
     1741 tcgtgaggtc ctgagcgtcg cggcgggtct gcagcgccga tgcctgggac acggtgacct
     1801 cgagatagct cgcggcaccg gcccggtacc ggttggtcga caggtccacg gaccgctggg
     1861 tcgcggcgac cgccgcggcc tccgacacgg ccgcctgccg gtagttgtcg agcagcgcga
     1921 ggctgtcctc cacttcctgg aacgcaccga gcaccacgct gcgatagttc gcgccggttt
     1981 cgtcgagcac ggcctccgcg tgcgcgacgt cggcctggcg tcggccgcca tcgaacaggt
     2041 ccatgagcag cgtcgggccg atcgcccaga acacgttggg ggcggtgacc aggttcgcga
     2101 actcggtgct ctggtaaccg taggtcgcgt cgagacggag ttgcgggaag aaggccgcct
     2161 tcgcgacacc gacgctcgca ttcgcggcct cgacgcgacg ctcggccgcc aggatgtcgg
     2221 gacgccgctg cagcagcgtg gaaggcacgt cgaccgggac gtcgggcaac gggatcgcgt
     2281 cggtccgcgg tgcgatcgag aagtccggcg cgggcacgcc cacctgcgag gcgatggcgt
     2341 gctcgacgac ggcgcgctgg ccgacgtact gcttcagcag cgagcgcgcc gcctcgagct
     2401 gggcctcggc ccgcgagacg tcgagtcccg aggcgatccc gcccacatga cgactctgga
     2461 ccagctcgag cgcgcggcgg aatgcggcct cggtctccgt cagcagctcg atctggcggt
     2521 ccagcccgcg caggatgaag tagctctcgg ccagcagggc ctgcaggctc aggcgcgccg
     2581 catcgaggtc cgcgcgggcg gcctcgtcga gcgcccggcc ggcggcgacc tcgttgctca
     2641 cgcggcccca caggtcgaac tcgtagccga gaccaaagcc tagcgccacc gagtcgtatt
     2701 cattcggcga gttgggcccg agcacccgca gcggcttcga ctccgactgc cggtcgcgct
     2761 gggcattgag cgcggtcgag agcgtcggga acaggttcga gcggatggcc tggtaagccg
     2821 cgttggcctc cttgtagcgg gccagcgccg cggcaagatc ggggctgttg gcgatcagct
     2881 gctgttcgag cgcgtcgagt tccggatcgc cgtacagcgt ccaccacgca tcgggctgca
     2941 gcggccgggc cgcctcggct cgttcaccgc cggacgcctc cttgtagctg gcgccgacgg
     3001 ggacactcgg cacctcgagc acgggcgcct gcgagcatgc cgcgagcgcc agcagcgtcg
     3061 cgagcgccgc cgcgagggac ttgcgactaa tgcccggcat gcccgtcctg gcccttcgca
     3121 ccggcagcgc ccggtttctc ggccggcgcc gcgacgcgca ccgcatcgcc attcgccacg
     3181 ccgtcgggag gattctcgat caccttgtcc gtcggggtga tgccggccga cagctcgacc
     3241 gtcgccccca tgtcgcgcga cagcgtgacc ggcttgagca cgaccttgtc gccggtgccc
     3301 agcgtcgcga cctgcagccc gtccttgtcg aagatcagtg cgctcgccgg gatgctcagg
     3361 gcgcccgcag cggagggaat gtcgaatcga acgcgcgcaa atccgccggg gagcagctcc
     3421 gagctcgcat tggcgaccgt cagctgcacg agcatgctgc cggaggccgg gtcgatggca
     3481 ttcgacgtgg actggaccgt cgccgcgtat tgccggcccg gccgttccgg gaccgtgagc
     3541 gtcgcctcgg tgccggggtg gacctgcgac gcgtagttct gcggcacgtt cacgtagaca
     3601 cggagcttgc ccaggtccgc gaccacgaac agttccgggc cctgcgcgct gccgacgttg
     3661 atcagtgcgc cgacgtcggt ctcacgacgg gtgaccacgc cgtcgaacgg cgcgacgatg
     3721 cgcgcgaact gcttcagcgc ctggtagcga tccacgttcg cctgcagcgc ccgcacctcg
     3781 gcctgcctgg ccgcgagatc cgcggtcttc tcctcgactt cctgctgcga gaccgagtcg
     3841 ctcgcgagca gcgactgcca acgcttcgcc gtggtctcgg ccagcgcagc attcgccctg
     3901 gcatttgcca gctccgcctg cgcctgcagc agctgctggt cgaggtccgg tgtctcgatc
     3961 tcggcgagca gctgtccggc gcggaccttg gtgccgatgt cggcgtacca ggccttgaga
     4021 tagccgctga cacgcgcgta gatcggcgcc tgtgcgaacg cctcgacccg gcccggcagc
     4081 tcgagtggtg cgccggccgc ggcaggcccg ggcgtgacga tcgtgacggt gggcaccgcg
     4141 gcatcggcgg tgcggtcgcg cagtcgctgc gcgtcgacga tccgcgtcgc cgccccgtac
     4201 acgacgacga cgaccgccgc gaaggcggcg atcgtgacga tccggcggac cgtgcgcgcc
     4261 ggcgacgcgg gcgacggggt atcagctggc atgaagttct cctggcatcg cggcctgccc
     4321 ggccagcgcc cgccggcggc cgtgtacgag gctgaagacg accggcacga agacgagcgt
     4381 ggcgaccgtc gcgaagcaca ggccgccgat cactgcgcgc ccgaggggtg cgttctgctc
     4441 gccgccttcg ccgaggccga gcgccatcgg cagcatgccg atgatcatcg cgagcgccgt
     4501 catgaggacc ggccggaagc gcacgaagcc ggcctcgagc gcggcctgcg cagggtcggc
     4561 gcccgcgtcg agccgctcgc gtgcgaaggc gatcaccagc acgctgttgg ccgtggcgat
     4621 gcccatgcac atgatggctc cggtgagcgc cggcaccgag agcgtcgtgt gcgtcgcgaa
     4681 cagcatccac acgatgccgg ccagcgcggc cggcagggcc gtgatgatga cgaacgggtc
     4741 cgtccacgac tggaaattca cgacgatcag caggtagatg agcacgatcg cgcccgccag
     4801 gccgaacagc aggcccgtga acgcgtgatc catggtgcgg acctgtccga ccagctcgac
     4861 cgacgagccc ttcgg
//
LOCUS       DAOMFD010000148         4862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7321_length_4862_cov_34.812357, whole genome shotgun sequence.
ACCESSION   DAOMFD010000148 DAOMFD010000000
VERSION     DAOMFD010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4862
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7321_length_4862_cov_34.812357"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /gene="dusB"
                     /locus_tag="PL152_08380"
     CDS             complement(<1..883)
                     /gene="dusB"
                     /locus_tag="PL152_08380"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014422716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HQR49340.1"
                     /translation="MDAIRIGPYDTGGRVLLAPMAGVTDLPFRRLCRRMGAALAAGEM
                     MSADPRLWSTAESIRRRDHSGEPEPRVVQIAGGDPAAMADAARRNVDAGAQVIDVNMG
                     CPAKKVCSKDAGSALLRDEQLVAAILDATVRAAGVPVTLKIRTGWSRERRNGVAIARI
                     AESAGVCALAVHGRSRACRFEGEAEYETIAAIKQAVRIPVIANGDIDSAVKAREVIRA
                     TGADGVMVGRAAQGRPWIFREIEAVLSGTAQPAAPAAAETRDIITAHLRDLYAFYGVE
                     AGVRIARKHIGWYCRERP"
     gene            complement(885..2168)
                     /locus_tag="PL152_08385"
     CDS             complement(885..2168)
                     /locus_tag="PL152_08385"
                     /inference="COORDINATES: protein motif:HMM:NF023334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon and DUF3426 domain-containing
                     protein"
                     /protein_id="HQR49341.1"
                     /translation="MYTQCPECNARFRVRAAALRAAGGTVRCGRCGSAFNALDRLDDA
                     VPPSDDDARRDPVAEAANDVAATPGPVTITEFHFTADDIEKVFVEGSAWQERSGAARA
                     DEAAGPTDAEDPLVLVSESQPVEDITLEGEQISIESILGVDDDLDSTAEFEILTDVPD
                     SAFPADSDAVAIEGAPPPAATEAALAPDSGPIPERTLDILLETAPRTARRAEPTPSAT
                     ATSATRPAEVASIAELRWRRSAEQSIPDFDADDERRGVSRSALAWSFGCLLLALGLLA
                     QVTHQFRQDLVRHPRIGPVLEDVYARLGVPLSPNWDLAAFELRRWSTASDADTGGRLT
                     VRASITNRAAFAQPHPILRLELDDRYGDSVAVRDFEPAEYLKNPSESARLLGPGASTE
                     AEVVIVKPGPDAVGYQLDACLRESAQLLRCAGNRT"
     gene            complement(2189..3067)
                     /gene="prmA"
                     /locus_tag="PL152_08390"
     CDS             complement(2189..3067)
                     /gene="prmA"
                     /locus_tag="PL152_08390"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HQR49342.1"
                     /translation="MPFLQVTLDIGSSDPAPFEDALAGLDAVAVTLEDAADDPVLEPA
                     PGATPLWPTVRLRALFDASADGRAVVSHIAARFPGLPPPQLVTIEDKAWEREWLKDFR
                     PMRFGRRLWVCPGGQPAGDADAVRIELDPGLAFGTGTHPTTALCLEWLDARDLHGCAV
                     VDYGCGSGILAIAALKLGAASVRAIDIDPQALIATRDNAFRNGVHAGLAVTSEPGLPP
                     GSVDILVANILAGPLAELAPRFGAAVRVGGELALSGVLTSQVDAVTAAYRPWFDIAFT
                     ATREGWGLIGGRRRAA"
     gene            3295..3699
                     /locus_tag="PL152_08395"
     CDS             3295..3699
                     /locus_tag="PL152_08395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49343.1"
                     /translation="MTDITNAGGRPAQVRVFSILLVASAIFMALLGWSAGAYYVPAMC
                     LVLLAALLWSGRAGGLFKWVLLVNQVAGLVLVLVLWLGDGLGAHKLDVSGVALLVNLL
                     CGGPLASILGVALLPGLRSGKRLATWFGARAA"
     gene            3714..>4862
                     /locus_tag="PL152_08400"
     CDS             3714..>4862
                     /locus_tag="PL152_08400"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HQR49344.1"
                     /translation="MNNIGRVILASCVLLSGAGSVAAASFTGKVTLPDGAPAYGAMLT
                     VFSADHAQRETVYTAADGTYAIVTPYSGTLTLRARLANYADATTSLDASQDAVGTADL
                     QLANFADDQAESDALSASAHNAKLQWQQAGDRRAFVSQCNYCHQIGNSTTRAPRSHDL
                     WMATITKMEGYFAMVTRAQKILFASVLEQGFDGKPVIAKHDYGATADLARAKIEEWNV
                     GDSFSFIHDMDVAEDGKFYGTDEGHDKLWILDPATGKVETADLPPSGLPRGGTFSGMH
                     LAIGIFTGSHGPHSMGQTSDGRIWITNSLSSTLMSFDPKTRQFKVYPVPGDALYPHTV
                     RVDRNDVVWFTIVASNQLGRFDPKTEQMTVMRLPHNGFFRWVTDMLFPT"
BASE COUNT          712 a         1716 c         1708 g          726 t
ORIGIN      
        1 gcggacgctc ccggcaatac cagccgatgt gcttgcgcgc gatcctgacc cccgcctcta
       61 cgccgtagaa tgcgtacaaa tcgcgcaagt gagccgtgat gatatcacgc gtttccgcgg
      121 cagcaggagc ggcgggttgt gccgtcccgg atagcacggc ctcgatctcg cggaagatcc
      181 acggccgccc ctgggctgcg cggccgacca tgacgccgtc ggcgccggtc gcgcgaatga
      241 cctcgcgggc cttgacagcc gagtcgatgt cgccgttggc gatcaccggg atgcgcaccg
      301 cctgcttgat cgcggcgatc gtctcgtatt ccgcctcgcc ctcgaagcgg cacgcgcggc
      361 tgcggccgtg cacggcgagc gcgcagacgc ccgcggactc cgcgattcgc gcgatcgcga
      421 cgccgttgcg ccgctcgcgg ctccagcccg tgcggatctt gagcgtgacc ggcacgcccg
      481 cggcgcgcac cgtggcgtcg aggatcgccg cgaccagttg ctcgtcgcgc aacagggccg
      541 acccggcgtc cttgctgcag accttcttcg ccgggcaacc catgttcacg tcgatcacct
      601 gcgcgccggc atccacgttg cggcgcgcgg cgtccgccat cgccgcgggg tcgcccccgg
      661 caatctgcac gacgcgcggc tcgggctccc cggagtgatc gcgccggcgg atcgactccg
      721 cggtggacca gagccgcggg tcggcggaca tcatctcgcc ggccgccagc gccgcgccca
      781 tccggcggca gagccggcga aacggcaggt cggtcacgcc cgccatcggg gcgagcagga
      841 cgcgcccgcc tgtgtcgtat gggccgatcc gaatggcgtc catctcaggt ccggttcccg
      901 gcgcagcgca gcaactgggc ggactcacgg aggcaggcgt cgagctggta accgaccgcg
      961 tcgggccccg gcttgacgat cacgacctcg gcctcggtgc tggcgcccgg gcccagcagg
     1021 cgcgcagatt ccgatggatt cttcagatac tccgccggct cgaagtcgcg cacggcgacg
     1081 gaatccccgt agcgatcgtc gagttccagg cgcaggatcg ggtgcggctg cgcgaatgcc
     1141 gctcgattcg tgatgctcgc gcgcactgtg agccggccgc ccgtgtcggc gtcgctcgcc
     1201 gtgctccagc ggcgcagctc gaatgccgcg aggtcccagt tgggcgacag gggcacgccg
     1261 agccgtgcgt acacgtcctc cagcacgggt ccgatgcggg ggtgtcgcac gaggtcctgc
     1321 ctgaactggt gcgtgacctg ggcgagcagg cccaacgcga gcaacaggca gccgaacgac
     1381 caggccagcg ccgaacggct caccccccgc cgctcgtcgt ccgcgtcgaa gtcggggatg
     1441 gattgctccg cggaacggcg ccagcgcagt tcggcgatgg acgccacctc cgccggcctg
     1501 gtcgcagacg tcgccgtcgc gcttggcgtc ggctcggccc gtcgcgccgt acgtggagca
     1561 gtctcgagca ggatgtcgag cgtgcgctcc ggaatcgggc ccgagtcggg cgcgagtgcc
     1621 gcctcggttg ccgccggcgg gggcgcgccc tcgatggcga cggcgtccga gtcggccgga
     1681 aacgcggagt ccggcacgtc cgtgaggatc tcgaattcgg ccgtggagtc caggtcgtcg
     1741 tccaccccga ggatggactc gatgctgatc tgctcgccct cgagcgtgat gtcctcgacc
     1801 ggctggctct cgctcaccag gacgagcgga tcctcggcgt ccgtgggtcc agctgcttca
     1861 tcggcgcggg cggcaccgct gcgctcctgc caggcggatc cttcgacgaa taccttctcg
     1921 atgtcgtccg cggtgaagtg gaattccgtg atggtcaccg ggccgggcgt cgctgcgaca
     1981 tcattggcgg cttccgcgac ggggtcgcgc cgtgcgtcgt cgtcggacgg gggtaccgcg
     2041 tcgtccagcc ggtcgagcgc gttgaacgcg ctgccgcagc gcccgcagcg caccgtgccg
     2101 ccggccgcgc gcaacgcggc cgcgcgcacc cggaagcggg cgttgcattc aggacattgc
     2161 gtgtacatca atcggctcgg ttgttccgtc aggccgcgcg tcgccgcccg ccgatcagcc
     2221 cccaaccttc gcgcgtcgcg gtgaaggcga tatcaaacca cggccggtaa gcggctgtca
     2281 cggcgtcgac ctgcgacgtg agcaccccgc tcaacgccag ttcgccgccc acccgcacgg
     2341 ccgcgccgaa tcgcggcgcg agctcggcga gcgggccggc caggatgttc gcgaccagga
     2401 tgtccaccga ccctggaggg agtcccggtt ccgaggtcac ggcgaggccc gcgtgcacgc
     2461 cgttgcgaaa cgcgttgtcg cgtgtcgcga tcagggcctg cggatcgatg tcgatcgcgc
     2521 gcacgctggc ggcgccgagc ttgagcgcgg caatggccag gatgccgctg ccgcatccgt
     2581 agtccacgac cgcgcagccg tggaggtccc gcgcgtcgag ccactcgagg cacagcgcgg
     2641 tcgtcgggtg agtcccggtg ccgaacgcga gtcccgggtc gagttcgatg cggacggcgt
     2701 cggcgtcacc ggcaggctgg ccgcccgggc agacccacag gcgacggccg aagcgcatcg
     2761 ggcggaagtc cttgagccat tcgcgttccc aggccttgtc ctcgatggtg accagctgcg
     2821 gaggcggcag gccgggaaac cgggcggcaa tatgggagac gacggcccgt ccgtcggcgg
     2881 acgcatcgaa gagtgctcgc agcctcaccg tcggccacaa cggcgtggcg cccggcgcgg
     2941 gctcgaggac cgggtcgtcc gcggcgtcct cgagcgtgac ggccaccgcg tcgagcccgg
     3001 ccagcgcgtc ctcgaacggc gccggatccg agctgccgat gtccagggtg acctgcagga
     3061 atggcatcgt cctaatcgtc cgctcgtcat ctcgcctgcg gcagggccgg cccgcttgtg
     3121 tccggggagg cgcaagggca tcctcgccac tccggacgcg cagcgtaaac gaagccggtg
     3181 cgcgcgggtc gcccggacga gcctcccccc cccctcatct ggaggaggta cgcccggaag
     3241 cccgcgcccg gaacatggcc gcccgagagc gtgactcaag aggggagacc tcaagtgact
     3301 gatatcacga atgccggcgg ccggccggca caggtccgcg tattcagcat cctgctggtc
     3361 gcgagcgcta tcttcatggc attgctcggc tggtcggccg gggcctacta cgtgccggcg
     3421 atgtgcctcg tgctgctggc ggcgctgctc tggtcggggc gcgcgggtgg cctgttcaag
     3481 tgggtcctcc tcgtcaacca ggtggccggc ctggtgctgg tcctcgtgct ctggctgggc
     3541 gacggactcg gcgcccacaa gctcgacgtg tcgggcgtcg cgctgctcgt gaacctgctc
     3601 tgcggaggcc cgctcgcgtc gatcctgggc gtcgcgctgc tgcccgggct gcgcagcggc
     3661 aagcgactgg cgacctggtt cggcgcgcgg gcggcgtgag gcgaggggcc agcatgaaca
     3721 acatcggtcg tgtgatcctg gcgtcgtgcg tcctcctctc cggcgctggc tcggtggcag
     3781 ccgcgtcgtt caccggcaag gtcacgctgc cggacggggc gcccgcctac ggcgcgatgc
     3841 tcacggtgtt cagcgccgac cacgcccagc gcgagaccgt ttacaccgcc gcggacggca
     3901 cgtacgcgat cgtgacgcct tactcaggca cgctcacgct gcgcgcaagg ctggccaact
     3961 acgcggacgc cacgaccagt ctggacgcgt cgcaggacgc agtggggacg gccgacctgc
     4021 agcttgccaa cttcgccgac gaccaggccg agtcggacgc gctgtcggca tcggcgcaca
     4081 acgcgaagct gcaatggcag caggccgggg accggcgcgc cttcgtgagc cagtgcaact
     4141 attgccacca gatcggcaat tccaccacgc gcgcgccgcg cagccacgac ctgtggatgg
     4201 ccaccatcac caagatggaa ggctattttg cgatggtcac cagggcgcag aagatcctct
     4261 tcgcgagcgt cctcgagcag gggttcgacg gcaagcccgt gatcgcgaag cacgactacg
     4321 gcgcgacggc cgacctggcg cgggcgaaga tcgaggagtg gaacgtcggc gactcgttct
     4381 cgttcatcca cgacatggac gtggccgagg acggcaagtt ctacggcacc gacgaggggc
     4441 acgacaagct gtggatcctc gatcccgcga cgggcaaggt cgagacggcc gacctgccgc
     4501 cgagcgggtt gccacgcggc ggcaccttct cgggcatgca cctggcgatc ggcatcttca
     4561 ccggcagcca cggcccgcac agcatgggac agacctccga cgggcgcatc tggatcacga
     4621 actcgctgtc gtcgacgctg atgtcgttcg atccaaagac ccggcagttc aaggtctatc
     4681 cggttccggg cgatgcgctg tacccgcaca cggtgcgcgt cgatcgcaac gacgtcgtct
     4741 ggttcacgat cgtggcctcc aaccagctcg ggcgcttcga cccgaagacc gagcagatga
     4801 ccgtcatgcg gctgccgcac aacggcttct tccgctgggt cacggacatg ctgttcccga
     4861 cg
//
LOCUS       DAOMFD010000149         4851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7351_length_4851_cov_39.242077, whole genome shotgun sequence.
ACCESSION   DAOMFD010000149 DAOMFD010000000
VERSION     DAOMFD010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4851
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7351_length_4851_cov_39.242077"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1161)
                     /locus_tag="PL152_08405"
     CDS             complement(1..1161)
                     /locus_tag="PL152_08405"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HQR49345.1"
                     /translation="MTSPAWTPPADHLAPVYAQWPVDIVGAEGVHLVTRDGRRILDLY
                     GGHAVAALGYSHPRWLAALEAQAKSLLFQSNAVPLEVRARAASRLSHFAGLGLNTVFF
                     GNTGAEANENALKLALRITGRRGVAAIEGSFHGRTAAAGAVTWGAAKKWYGFPQAPFD
                     VAFIPRGDTDAITRVVDDRTAAVIVEPVQGVAGAVDLGGPFLAALRTRCSEAGAMLVC
                     DEVQCGMGRTGFPFASSMHGVAPDMITTAKALGAGFPCSALLMADHVAERLATDDLGT
                     TFGGGPMACALIEAVVDIIESEGLLANVRARAQQLRETCVVGPVESVQGAGLLAGLRT
                     RRPARDVQRELLEHDILTGTSADPNVVRVLAPYVLEESHVATLRDVLASLPA"
     gene            complement(1190..2251)
                     /gene="argC"
                     /locus_tag="PL152_08410"
     CDS             complement(1190..2251)
                     /gene="argC"
                     /locus_tag="PL152_08410"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: protein motif:HMM:TIGR01850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HQR49346.1"
                     /translation="MTTRIPAVVLGGTGYVAGELLRLVASHPYLDLAAVASESQPDEP
                     VAQFFRHLQPLLPHLRFAGHDEVLEYVTANPVTAVFSAAPHGVSAGLVDRLLTASAQA
                     GTRTRVVDISADFRYPTAEAYEAVYKHPHGAPARLAEFTCAVPEHLNALHTPHVAHPG
                     CFATAILLASVPLLSLGLAEPRVFVTGITGSTGSGRQPTAGTHHPQRHSDLYAYNALS
                     HRHVPEVAALARVATGVDAEFNFVPHSGPFARGIHVTVQAVLREPLTTHELVGALRDY
                     YDQSVFVRVGGELPRIKDVVASNFAELSGVARGTSVAVMCAIDNLTKGAAGGAVQWMN
                     RMLGYEETAGLMTPAAGWT"
     gene            complement(2248..3339)
                     /locus_tag="PL152_08415"
     CDS             complement(2248..3339)
                     /locus_tag="PL152_08415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49347.1"
                     /translation="MVAQMRTVVVDKIASIAQACGLSHELRVATSDIPCEEGVVLVVE
                     ILTNKSTYNQLELTSGRMAKVARGDVIVGALGHRQALFGYSGHIPDKVEAGDVLQVLN
                     IGGVLGVCDSVNPDKGKPFDCRVIGCVLQFPFLGERIGVPARVGHRRLDQAAPLDTHG
                     VPVVALAGTCMEAGKTAAACAIVARMRHRGLAVHAFKATGVSLRRDILAMEDSGARRS
                     MIFTDLGIVTTTAASGPALTRTMLTEMTQGRPDVVVFELGDGILGAYGVESILAARDI
                     RDALSAVILSANDPVAAWGGVKLLRERFGIEPCAVTGPATDNAVGVNIIEQQMQVRAM
                     NAMSSGAALGDHIIESLGLASRAAGGTVE"
     gene            complement(3339..4565)
                     /gene="argG"
                     /locus_tag="PL152_08420"
     CDS             complement(3339..4565)
                     /gene="argG"
                     /locus_tag="PL152_08420"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HQR49348.1"
                     /translation="MTQTTAGAKPILLAFSGGLDTSFCVPWLAETYGRPVVTLTVDTG
                     GIDAGAASVLEERSRALGAVAHHLVDARRAYFDQVIRFLIMGNVRRGGLYPLCVGAER
                     VLQAQTVAQWARKLGTEVVAHGSTAAGNDQVRFEVALRTLAPGLEILAPVRDRAFKRP
                     EQLEYLESRKLPVPPFGAAYSVNRGLWGTTIGGKETLTSDGCIPDSAWVTTRDAFTNP
                     QPPERHTIGFDAGIPSSLDGAKLDPVALIEQVEAIGARYGVGRGIHLGDTIIGFKGRV
                     AFEAPAAEALLTAHRELEKLVLSARQARLKDTLAAPYGDLVHEGQHLDPVCRDIEAFL
                     LSSQQRVTGEVHLLYRPGSVFVEGVSSPWSLMKASKGAYGESAGEWTAAEAHGFSKML
                     ALPGIFYTRAGQQGAG"
     gene            complement(4573..>4851)
                     /locus_tag="PL152_08425"
     CDS             complement(4573..>4851)
                     /locus_tag="PL152_08425"
                     /inference="COORDINATES: protein motif:HMM:NF014864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49349.1"
                     /translation="DRYLLPAAEDALTPSHFEDVRAFVKGYRAAGAALTVLRTTAGAA
                     QSVALAIDKAHWPEIVGTIAGDDTIFIATGNARAQRRLHQHLRTSFGR"
BASE COUNT          704 a         1714 c         1748 g          685 t
ORIGIN      
        1 tcaggcgggg agcgaggcga gcacgtcacg caatgtcgcg acgtggcttt cctcgagcac
       61 gtacggggcg agcacgcgca cgacgttcgg gtccgcgctc gtgccggtca ggatgtcgtg
      121 ctccaggagt tcgcgctgca cgtcccgggc cggccggcgg gtcctcaggc cggcgagcag
      181 ccctgcgccc tgcaccgact cgacgggacc cacgacgcag gtctcgcgca gctgctgcgc
      241 gcgggcccgc acgttggcga gcaacccctc cgattcgatg atgtccacga ccgcctcgat
      301 gagcgcgcag gccatcggtc caccgccgaa ggtcgtgccg aggtcgtcgg tcgcgagccg
      361 ctccgccacg tggtcggcca tgagcagtgc cgagcaggga aacccggcgc cgagcgcctt
      421 ggcggtcgtg atcatgtccg gcgccacgcc gtgcatgctg gaggcgaacg ggaacccggt
      481 ccggcccatg ccgcactgca cctcgtcgca gaccagcatc gcgccggcct cgctgcacct
      541 cgtgcgcagc gcggcgagga acgggccgcc gagatccacg gcaccggcca cgccctgcac
      601 gggctcgacg atcaccgccg cggtgcggtc gtcaaccacg cgcgtgatcg cgtccgtgtc
      661 gccgcgcggg atgaatgcga cgtcgaaagg cgcctgcggg aagccgtacc acttcttcgc
      721 cgcgccccag gtcaccgcgc cggccgcggc cgtgcgcccg tggaagctgc cctcgattgc
      781 cgcgaccccg cgtcggccgg tgatccgcaa cgcgagcttg agcgcattct cgttggcttc
      841 cgcgccggtg ttcccgaaga acacggtgtt gagtcccagg cccgcgaagt gggagagcct
      901 ggatgcggcg cgggcacgca cctcgagcgg cacggcgttc gactggaaca gcagcgactt
      961 cgcctgcgcc tccagcgccg cgagccaccg tggatgcgag tagccgagcg cggcgaccgc
     1021 gtggccgccg tagaggtcga ggatccgccg gccatcgcga gtgacgaggt gcacgccctc
     1081 cgccccgacg atgtcgaccg gccactgtgc atagaccggc gccaggtgat cggcgggcgg
     1141 cgtccaggcc ggactggtca cgaatgattc cttcggcatg ggcgtggcgt caggtccagc
     1201 ccgcggcggg cgtcatcagc cccgcggtct cctcgtaccc gagcatgcgg ttcatccact
     1261 gcacggcgcc gcccgctgcg cccttggtga ggttgtcgat cgcgcacatg acggccacgc
     1321 tggttccacg ggcgacgccc gagagctccg cgaagttgct cgcgaccacg tccttgatcc
     1381 gcggcaactc gccgccgacg cggacgaata ccgactggtc gtagtagtcg cgcagcgcac
     1441 cgaccagctc gtgcgtggtg agcggctcgc gcaggacggc ctgcaccgtg acgtggatgc
     1501 cgcgtgcaaa cggaccggag tgcgggacga agttgaactc ggcgtccacg cccgttgcga
     1561 cgcgtgccag cgcggcgacc tccggcacgt ggcgatgcga cagcgcgttg tacgcgtaga
     1621 ggtcgctgtg gcgttgcgga tggtgcgtcc cggcggtcgg ctggcgaccc gagccggtgc
     1681 tgccggtgat gcccgtgacg aacacgcgcg gttcggcgag cccgagcgac agcagcggca
     1741 cgctcgcgag caggatggcc gtcgcgaagc acccggggtg cgcgacgtgc ggcgtgtgca
     1801 gcgcattgag gtgttccggc accgcgcagg tgaactccgc gagtcgcgcg ggcgcgccgt
     1861 gcggatgctt gtagaccgcc tcgtacgcct cggcggtcgg gtagcggaag tcagccgaga
     1921 tatcgacgac tcgggtccgg gtgccggcct gcgcggacgc ggtgagcaga cggtccacga
     1981 gcccggccga cactccgtgc ggcgccgccg agaacaccgc ggtcaccgga ttcgcggtca
     2041 cgtactcgag cacctcgtcg tggccggcga agcgcaggtg cggcagcagc ggctgcaggt
     2101 ggcggaagaa ctgcgccacg ggctcgtccg gctggctctc ggaggccacg gcggcgaggt
     2161 cgaggtaggg gtggctcgcg accaggcgca gcagctcgcc cgcgacgtaa ccggtgccgc
     2221 cgagcacgac ggccgggatg cgcgtggtca ttcgacggtc cctcccgcgg cacgcgaggc
     2281 gagccccagg ctctcgatga tgtggtcgcc gagcgcggcg cccgacgaca tcgcgttcat
     2341 cgcgcgcacc tgcatctgct gctcgatgat gttgacgccg acggcattgt cggtcgcggg
     2401 gccggtcacg gcgcacggtt cgatgccgaa gcgctcgcgc agcagcttga cgccgcccca
     2461 ggccgcgacg gggtcgttgg cggacaggat caccgcggac agcgcgtcgc ggatgtcgcg
     2521 cgcggcgagg atggactcga cgccgtatgc gccgaggatg ccgtcgccga gctcgaacac
     2581 cacgacgtcg gggcggccct gcgtcatctc cgtcagcatc gtgcgggtga gcgccgggcc
     2641 cgacgccgcg gtggtcgtca cgatcccgag gtcggtgaag atcatgctgc ggcgcgcgcc
     2701 cgagtcctcc atcgccagga tgtcgcggcg cagggacaca ccggtcgcct tgaacgcgtg
     2761 caccgcgagg ccgcggtgcc gcatgcgcgc gacgatggcg caggcggccg ccgtcttgcc
     2821 ggcttccatg caggtgccgg ccagcgccac gacgggtacg ccatgcgtgt cgagcggcgc
     2881 ggcctggtcc agccgtcgat gaccgaccct ggccggcacg ccgatgcgct cgccgaggaa
     2941 cgggaactgc agcacgcaac cgatcacgcg gcagtcgaac ggcttgccct tgtcgggatt
     3001 caccgagtcg cacacgccga gcacgccgcc gatgttgagc acctgcagga cgtcgccggc
     3061 ctcgaccttg tcggggatgt gcccggagta gccgaacagc gcctgccggt ggccgagcgc
     3121 gccgacgatg acgtcgcccc tcgccacctt ggccattcgg ccgctggtga gctcgagctg
     3181 gttgtacgtg gacttgttgg tgaggatctc caccaccagc accacgcctt cctcgcaggg
     3241 aatgtcgctc gtcgcgaccc ggagctcgtg cgacaggccg cacgcctgcg cgatcgaggc
     3301 gatcttgtcg acgacgacgg tgcgcatctg ggcgaccatc agcccgctcc ctgctgtccg
     3361 gcgcgcgtgt agaagatccc cggcagcgcc agcatcttcg agaacccgtg cgcctcggcc
     3421 gcggtccact cgccggcgga ctcgccgtac gcgcccttcg aggccttcat gagcgaccac
     3481 ggcgagctca cgccttcgac gaacaccgag cccggcctgt agagcaggtg cacctcgccc
     3541 gtcacgcgct gctgggacga gagcaggaac gcctcgatgt cgcggcacac cgggtcgagg
     3601 tgctggccct cgtgcacgag gtcgccgtac ggagccgcga gcgtgtcctt caggcgcgcc
     3661 tgccgcgccg acagcaccag cttctcgagc tcgcgatgcg ccgtgagcag tgcctccgcc
     3721 gccggcgcct cgaacgcgac ccggcccttg aagccgatga tggtgtcgcc gaggtggatg
     3781 ccgcggccca cgccgtacct ggcgccgatc gcctcgacct gctcgatcag cgcgacggga
     3841 tcgagctttg cgccgtccag cgaactcgga atgccggcgt cgaagccgat cgtgtgtcgc
     3901 tcgggaggct gcgggttcgt gaacgcgtcc cgggtcgtca cccacgccga atcgggaatg
     3961 cagccgtcgg acgtcagcgt ctccttgccg ccgatggtcg tgccccacag gccgcgattg
     4021 accgaatagg ccgcgccgaa tggcggcacc ggcagcttgc gcgactcgag atactcgagc
     4081 tgctccggcc gcttgaatgc gcggtcacgg accggagcga ggatctcgag tcccggggcg
     4141 agcgtgcgca gcgccacctc gaagcgcacc tggtcgttgc cggcggcggt ggaaccgtgc
     4201 gccacgacct ccgttccgag cttgcgcgcc cactgcgcca cggtctgcgc ctgcagcacg
     4261 cgttcggcgc cgacgcacag cggatacagt ccgccgcggc gcacgttccc catgatcagg
     4321 aagcggatca cctggtcgaa atacgcgcga cgcgcatcca cgaggtggtg ggcgacggcg
     4381 ccgagcgcgc gagagcgctc ctcgagcacg gaggccgcgc ccgcgtcgat gccgccggtg
     4441 tcgaccgtca gcgtgaccac cggccggccg taggtctccg cgagccacgg cacgcagaag
     4501 gaggtgtcga ggccgccgga aaatgcgagc agtatgggct tggcgcccgc ggtggtctgg
     4561 gtcatgggag cctcagcgtc cgaaggaggt gcgtaggtgc tggtgcagcc gacgctgcgc
     4621 ccgcgcattg cccgtcgcga tgaagatggt gtcgtcgccg gcgatcgtcc cgacgatctc
     4681 cggccagtgc gccttgtcga tggcgagcgc gacgctctgg gcggccccgg cggtggtgcg
     4741 cagcaccgtc aacgccgcgc ccgccgcccg gtagcccttc acgaaggcgc gcacgtcctc
     4801 gaaatggctc ggcgtgagcg cgtcctcggc ggcgggcagc aggtagcggt c
//
LOCUS       DAOMFD010000150         4845 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7365_length_4845_cov_36.202923, whole genome shotgun sequence.
ACCESSION   DAOMFD010000150 DAOMFD010000000
VERSION     DAOMFD010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4845)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4845
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7365_length_4845_cov_36.202923"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1072)
                     /locus_tag="PL152_08430"
     CDS             complement(<1..1072)
                     /locus_tag="PL152_08430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49350.1"
                     /translation="MTNRAILVFSSLLLFAYLLDIVGRRFRLPAVVLLIVTGIVARQV
                     LDRIGVVLGWVDPLVPVIGTIGLVLIVLEGALDLSLRRERAALVWRASVAAGIGFVAC
                     TAAFTYAFEAGFGLPRVAAVLVAMPFAVISSAVAIPSATGLAQDAREFVTYESSISDI
                     LGVLVFYAWLGAQGDLGAFAYDLFGGGAISLAAALAAGVGLLYLINRIEGHVRFLPLL
                     AGLLLLYSVGKALHLSPLVLVLVCGLLLNNPHLFERSRRLGRLFKDGYDDTLAEFKGL
                     VVELTFAVRSFFFLMLGYWTELGHMGEWRAWAAAALVVAVIYATRWAILRALGQSESG
                     RLLWLAPRGLITVLLFLAAAETG"
     gene            complement(1086..1550)
                     /gene="hybE"
                     /locus_tag="PL152_08435"
     CDS             complement(1086..1550)
                     /gene="hybE"
                     /locus_tag="PL152_08435"
                     /inference="COORDINATES: protein motif:HMM:NF023365.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[NiFe]-hydrogenase assembly chaperone HybE"
                     /protein_id="HQR49351.1"
                     /translation="MLGSADGASPLSHPVARVANAYRRVALTMKGLPIYNPTLAVEAI
                     GFREHEGRHVGVVVTPWFMNLTVLPSDADQGVWVSGGTVRLTFPSGQYDLLVGELPEF
                     GLVGTCALFSPMSEFTDHEAAQVAARAAADALFQPEEPPPAPTYSRRQLLGG"
     gene            complement(1545..1754)
                     /locus_tag="PL152_08440"
                     /pseudo
     CDS             complement(1545..1754)
                     /locus_tag="PL152_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647937.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rubredoxin"
     gene            complement(1751..2599)
                     /locus_tag="PL152_08445"
     CDS             complement(1751..2599)
                     /locus_tag="PL152_08445"
                     /inference="COORDINATES: protein motif:HMM:NF016682.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HQR49352.1"
                     /translation="MTDYPLVWHPKVPTESSATGDESLRLLDMPLGSARPRREARRVD
                     RLSAEARAVLEEALATLRRHAEGAHEASRISLRPLSPADRDAVLDLLGEGDVWGSVGG
                     NVAYAFVETVLTGLWRIEATGQDGAKTEWLEVAEIPQPVLHATELMPRDAVRIPDQLP
                     QGAMNSAALLTELQERSSGYRAGEENHVINFTLLPITEADAEVLTTVLGQIPLVIRGG
                     GYGSCRIFATGLRHVWAVQYLNSMDKVILDTLEVGGVPVAALAAREDFEDSAGRLAEM
                     LQAYVS"
     gene            complement(2596..2997)
                     /locus_tag="PL152_08450"
     CDS             complement(2596..2997)
                     /locus_tag="PL152_08450"
                     /inference="COORDINATES: protein motif:HMM:NF019092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49353.1"
                     /translation="MSEILESLAASDGCEVVRKADLDAFLASHPRAVIFMAGDTVQRQ
                     EGLDVAVVVRELASSYRGRIAVGLVDRRDESHVMSRFGVVVLPAVVFVRDGQAAELVA
                     RMRDWPVYAQACDRLLAPDGGAGSAGVGGNA"
     gene            complement(2994..3755)
                     /locus_tag="PL152_08455"
     CDS             complement(2994..3755)
                     /locus_tag="PL152_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HQR49354.1"
                     /translation="MAELRLGPAQWLTYAARGPGNDGRHSGRMTTGGSDPAGRRIKVL
                     GVGNLLWADEGFGVRCIEALGAAWEFPPEVELLDGGTLGLALIPLLQDATHVLLFDAV
                     DFGGEPATLIVARDRDVPRFMTRDKMSLHQAGMNDVLAMLDMLGHRPQAFTLVGVQPV
                     ELADYGGSLTPRVLALLPEALELGLEELAAWGVAPQPLAQAAAGTLTSEAIGLDRYET
                     ERPTEQAAFRGGDERFLGRRTGSTQRGPGDPEKTP"
     gene            complement(3844..4581)
                     /gene="cybH"
                     /locus_tag="PL152_08460"
     CDS             complement(3844..4581)
                     /gene="cybH"
                     /locus_tag="PL152_08460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase, b-type cytochrome subunit"
                     /protein_id="HQR49355.1"
                     /translation="MTYAYPSTPAKSRVREGKFSEAILPVGEQPGPVYVYDAATRIWH
                     WVTMLSIITLCVTGYFIGSPLPSIGGEAYDHFLFGNIRMVHFIAGQVLGVVFVMRLYR
                     VLVGGPHARQIFYIPFWNLHWWKDVIDEIGWYLFLKKPKEYVGHNPLAHLAMFLMFVL
                     PTIVLLLTGFALFAEGAGIQSLWYKAFGWVFLVIGNSFSVHTWHHVAMWIVILFSIVH
                     MYMAIREDMTHRQTTVSSMISGWRFFR"
     gene            complement(4592..>4845)
                     /locus_tag="PL152_08465"
     CDS             complement(4592..>4845)
                     /locus_tag="PL152_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HQR49356.1"
                     /translation="WIDIDDMKIKNYQCIVPTTWNASPRDGAGNIGAYEAALLNTPMA
                     DPEKPLEILRTIHSFDPCLACATHVMDMDGNEVSSVKVR"
BASE COUNT          792 a         1729 c         1638 g          686 t
ORIGIN      
        1 ttcccgtctc ggcggccgcc aggaacagca gcaccgtgat cagcccgcgc ggcgcaagcc
       61 acagcagccg gcccgactcg gactggccga gcgcccgcag gatcgcccag cgcgtcgcgt
      121 agatcaccgc gacgaccagt gccgcggcgg cccaggcgcg ccactcgccc atgtgcccga
      181 gctcggtcca gtagcccagc atcaggaaga agaagctgcg gaccgcgaac gtcagctcga
      241 cgaccaggcc cttgaactcg gcgagcgtgt cgtcgtaacc gtccttgaag agcctgccga
      301 gccgccggct gcgctcgaag aggtgcgggt tgttgagcag caggccgcac accagcacca
      361 gcaccagcgg ggacaggtgc agtgccttgc cgaccgagta cagcagcagc aggccggcga
      421 gcagcggcag gaagcgcacg tggccttcga tgcgattgat caggtacagc aggccaacgc
      481 ccgcggccag cgccgccgcg agggagatcg cgccgccgcc gaacaggtcg taggcgaagg
      541 cgcccagatc gccctgtgcc cccagccagg catagaacac cagcacgccg aggatgtcgg
      601 agatcgaact ctcgtaggtc acgaactcgc gggcgtcctg cgcgaggccc gtcgcgctcg
      661 ggatggccac ggcgctcgag attacggcga acggcatcgc caccagtaca gccgcgacac
      721 gcggcaggcc gaagcccgcc tcgaacgcgt aggtgaacgc cgcggtgcag gcgacgaagc
      781 cgatgcctgc tgcgaccgac gctcgccaca cgagcgcggc gcgctcgcgg cgcagcgaca
      841 ggtcgagcgc gccttcgagc acgatcagga ccaggccgat cgtcccgatg accgggacca
      901 gcgggtccac ccagcccagg accacgccga tccggtccag cacctgccgc gccacgatgc
      961 cggtcacgat gagcaggacg acggcgggca gcctgaagcg ccggccgacg atgtcgagca
     1021 ggtaggcgaa cagcagcagg gacgagaaga ccaggatcgc gcgattcgtc atgtcccggc
     1081 gagcctcacc cgccgagcag ctgccgtcgc gagtacgtcg gggccggggg cggctcctcc
     1141 ggctggaaca gcgcatcggc ggcggcacgc gccgcgacct gcgccgcctc gtggtcggtg
     1201 aactcgctca tcggcgagaa cagtgcgcag gtgcccacga gtccgaactc aggcagttcg
     1261 cccaccagca ggtcgtactg gccggacggg aacgtgagcc gcacggtccc gcccgagacc
     1321 cacacgccct ggtccgcgtc cgacgggagc accgtgaggt tcatgaacca cggcgtgacc
     1381 accacgccga cgtggcgtcc ctcgtgctcg cggaatccga tcgcctcgac cgcgagcgtc
     1441 gggttgtaga tcggcagtcc cttcatcgtg agcgcgaccc ggcggtaggc gttggcgacc
     1501 cgcgccaccg ggtgggagag gggtgaagcg ccgtccgcgc tgccgagcac caggaacttg
     1561 ctcttctccg cgtcgcagtt ggggcagcgc cagtgatccg gcagcagcgc gaacggcgtg
     1621 ccgggcgcga cctgcgtcac ctcgtcgccg agcgccgggt cgtacaccca ccagcagacg
     1681 ccgcactcga ggcgcgtgtc gtcgccgatc ttcgagttgt ccccgaggta gctgccggtc
     1741 gaggaactgc tcatgacacg taggcctgca gcatctccgc gagccggccg gcggagtcct
     1801 cgaagtcctc gcgcgccgcg agcgccgcga cgggcacgcc cccgacctcg agcgtgtcca
     1861 ggatcacctt gtccatcgag ttcaggtact gcacggccca cacgtgccgg agccccgtcg
     1921 cgaagatgcg gcagctgccg tagccgccgc cccggatcac cagcgggatc tggccgagca
     1981 cggtcgtcag cacctccgca tcggcctccg tgatcggcag caacgtgaag ttgatgacgt
     2041 ggttttcctc gcccgcgcgg tagcccgagg accgctcctg cagctccgtg agcagcgccg
     2101 cggaattcat ggcgccctgc ggcagctggt ccgggatgcg gacggcatcg cgcggcatga
     2161 gctcggtggc gtgcagcacc ggctggggga tctccgcgac ctcgagccac tcggtcttcg
     2221 cgccgtcctg cccggtggcc tcgatgcgcc acaggccggt cagcaccgtc tcgacgaatg
     2281 cgtaggcgac gttgccgccg accgatcccc agacgtcgcc ctcgccgagc aggtcgagca
     2341 cggcatcccg atccgcgggg gagagcggtc gcaggctgat cctgctcgcc tcgtgcgcgc
     2401 cttccgcatg cctgcgcagt gtcgccaacg cttcctcgag caccgcgcgc gcctcggcgg
     2461 acaggcggtc cacgcgccgt gcctcgcggc gcggtcgcgc ggatccgagc ggcatgtcga
     2521 gcagccggag cgactcgtcg ccggtcgcgg acgactcggt gggcaccttc gggtgccaga
     2581 ccagcggata gtccgtcatg cattgcctcc cacgccggct gatccggcgc caccgtcagg
     2641 cgcgagcaat cgatcgcagg cctgtgcgta cacgggccag tccctcatgc gggcgaccag
     2701 ctcggccgcc tgcccgtcgc gcacgaacac cacggccggc agcaccacga cgccgaagcg
     2761 tgacatcaca tgcgactcgt cccgccggtc gacgagcccc acggcgatgc ggccgcggta
     2821 gctcgaggcc agctctcgca ccacgaccgc cacgtccagg ccttcctggc gctgcaccgt
     2881 gtcgccagcc atgaagatca ccgcccgggg atgactcgcg aggaatgcgt cgaggtcggc
     2941 cttgcgcacg acctcgcagc cgtcgctggc cgccaggctt tccaggatct cgctcatggc
     3001 gtcttctccg ggtcgccggg cccacgttgc gtcgaaccgg tgcgccggcc caggaagcgc
     3061 tcgtcgcctc cacggaaggc cgcctgctcg gtcgggcgct cggtctcgta gcgatcgagg
     3121 ccgatagctt cggacgtgag ggtgccggcg gctgcctgcg cgagcggctg cggtgccacg
     3181 ccccaggccg cgagctcctc gagccccagt tcgagggcct ccggcagcaa tgcgagcacg
     3241 cgcggggtga ggctcccgcc gtagtcggcg agttccaccg gctgcacgcc gaccagcgtg
     3301 aatgcctgcg gccggtgacc gagcatgtcg agcatggcca gcacgtcgtt catgccggcc
     3361 tggtgcaggc tcatcttgtc ccgggtcatg aagcgaggga cgtcccggtc acgggccacg
     3421 atcagcgtgg cgggctcgcc cccgaaatcc accgcgtcga acagcaggac gtgcgtcgcg
     3481 tcctgcagca gcgggatgag cgcgagcccg agcgtgccgc cgtcgagcag ctcgacctcg
     3541 ggcgggaatt cccacgccgc gccgagcgcc tcgatgcagc gcacgccgaa gccttcgtcg
     3601 gcccacaaca ggttgccgac gccgagcacc ttgatgcggc gcccggccgg atccgatcct
     3661 cccgtcgtca tgcgtcccga gtgcctcccg tcgttgcccg gaccgcgagc ggcatatgtt
     3721 agccactgcg ccgggccaag tctcagttcc gccatggccg ggcggcggcc ttgccgcccc
     3781 ggacaagcaa cggggccggt ctctcgagcc ggccccggtt gcgcgaacgc ctgcgccgtc
     3841 aggtcagcgg aagaatctcc agccgctgat catcgagctc acggtcgtct ggcggtgcgt
     3901 catgtcctcg cggatcgcca tgtacatgtg gacgatcgag aacaggatca cgatccacat
     3961 cgcgacgtgg tgccaggtgt gcacggagaa ggagttcccg atcaccagga acacccagcc
     4021 gaacgccttg taccacaggc tctggatgcc ggcgccctcc gcgaacaggg cgaaccccgt
     4081 gagcagcagg acgatggtcg gcagcacgaa catcaggaac atcgcgaggt gggccagcgg
     4141 gttgtgcccc acgtactcct tgggcttctt gaggaacagg taccacccga tctcgtcgat
     4201 gacgtccttc caccagtgca ggttccagaa cgggatgtag aagatctgcc gcgcatgcgg
     4261 gccgccgacc agcacccggt agaggcgcat cacgaacacc acgcccagca cctgtccggc
     4321 gatgaagtgc accatgcgga tgttgccgaa caggaagtgg tcgtaggcct caccgccgat
     4381 gctgggcagc ggtgacccga tgaagtagcc cgtcacgcac agggtgatga tcgacagcat
     4441 cgtgacccag tgccagatgc gcgttgcggc gtcgtagacg tagaccggcc ccggctgctc
     4501 gccgacgggc aggatcgcct cgctgaactt gccttcgcgc acccggctct tggccggcgt
     4561 cgaggggtaa gcgtaggtca tggccacctc ctcagcgcac cttgacgctg ctgacttcgt
     4621 tgccgtccat gtccatcacg tgcgtcgcgc aggccaggca cggatcgaag ctgtggatcg
     4681 tgcgcaggat ctcgagcggc ttctcgggat ccgccatcgg cgtgttgagg agtgccgcct
     4741 cgtaggcgcc gatgttgccc gcgccgtcgc gggggctcgc attccaggtc gtcggcacga
     4801 tgcactggta gttcttgatc ttcatgtcgt cgatgtcgat ccagt
//
LOCUS       DAOMFD010000151         4838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7378_length_4838_cov_36.151579, whole genome shotgun sequence.
ACCESSION   DAOMFD010000151 DAOMFD010000000
VERSION     DAOMFD010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4838
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7378_length_4838_cov_36.151579"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1607
                     /gene="ettA"
                     /locus_tag="PL152_08470"
     CDS             <1..1607
                     /gene="ettA"
                     /locus_tag="PL152_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009392033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HQR49357.1"
                     /translation="LRDISLSFFPGAKIGVLGLNGSGKSTLLRIMAGVDKEFEGEARP
                     QPGIKIGFLSQEPQLDPAMDVRGNVEVAVAGIKEALAKLDAVYAAYAEPDADFDRIAA
                     EQARLEAIIQAADGHNLDRQLEIAADALRLPPWDADVTKISGGERRRVALCQLLLSKP
                     DMLLLDEPTNHLDAESVRWLEHYLEQYPGTVIAVTHDRYFLDNVAGWILELDRGFGIP
                     WQGNYSSWLEQKEKRLQVEEQQQEALKKMLARELEWVRQNPKARQAKSKARMQRFEEL
                     QSREFQERNETNEIYIPPGPRLGELVIEAKGLSKAYGEKILFENLNFNLPQGGIVGII
                     GPNGAGKTTLFRILTGQEQADGGEIRIGPTVQLAYVDQSRQSLDDKKTVWQEISNGLD
                     MLKVGNYETPSRAYVGRFNFRGSQQQQLIGELSGGERNRVHLAKVLKSGGNVVLLDEP
                     TNDLDVETLRALEEALLSFPGCAVVISHDRWFLDRIATHILAFEGNSEVVWFEGNYSE
                     YVEDLKRRRGEDAEQPHRMRYRSPHA"
     gene            1636..3156
                     /gene="hutH"
                     /locus_tag="PL152_08475"
     CDS             1636..3156
                     /gene="hutH"
                     /locus_tag="PL152_08475"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HQR49358.1"
                     /translation="MARSKTRSALEIDGKELTLDRLRAFESRRPRVTLAAAARKRMQG
                     SRDAVRRAIDRGDVSYGINTGFGAFANKVIPAANTRKLQLNLIRSHACGTGQPLPEPI
                     VRRMLLLKAHSLAAGHSGARPLAAEMLLALLNSDVLPIVPSQGSVGASGDLAPLAHVA
                     LALAGEGEAMHDGKRLSGREVLDAVGLEPLGLEAKEGLALLNGTQLSLALALEGLFQA
                     EHLLDASIVAGALTVEGLAGSHVPFDARIQEASRLPGQVKVGERFRRLLTESEIRRSH
                     ADCERVQDPYAVRCMPQVFGAVEHTLEHARAVLGAAVNGVSDNPLIFGDDILSGGNFH
                     AEPVGFVSDFLAIAVAELASIAERRIDLLDRRVNPNLNMFMAAEPGLESGFMMAHVTA
                     AALVSENKTYAHPASVDSLPTSAGQEDHVSMAPWAGRKLLAVCANTATVLGIELLAAA
                     QAIDAMRPLTTTPELQRVHALVRERVPYRAHDHRLDRDIEALAALVREGTLSSFIA"
     gene            3162..3806
                     /locus_tag="PL152_08480"
     CDS             3162..3806
                     /locus_tag="PL152_08480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49359.1"
                     /translation="MRGVAVTLAGFAFIALLLAWSRWLTRHRLAAVANLALATIAAIV
                     AAALWSVATGLEDYEPLRAETVVAEVRFDETGTDRYRATFMHLPEGGVQVFEMPGNRW
                     RLAARTLEWHGFAADAGLKPMFRFEALEAGTATTDGEGVRVLRTYQLAEPDSLDVWAW
                     VRASRAWSRCAVAGRADGAWLPVSRGEHFVVRIADGVLRIEPAAGPTPTLAANR"
     gene            3872..4588
                     /locus_tag="PL152_08485"
     CDS             3872..4588
                     /locus_tag="PL152_08485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49360.1"
                     /translation="MSVHVDSSGRLHHDQERSLALNCPHCQVFSHITAVSVPSYSELV
                     ANRPSQVGIVYRCDSCNAPIFLRFAVKMYAANRVELANNFVELERAREKFNFTYLPEE
                     SEPVFREALGCYSAGYFNAFASMCRRTAQVVFADLGENGRLRIFNQINEAREMAEIDG
                     DTFAVVKKVLFGTDADAPPSLPEIGAYEAGVLLELVKDMLYQAYVRKGKLQQAMMVRR
                     FFAEETLDKITPIGRAAGAD"
BASE COUNT          780 a         1698 c         1661 g          699 t
ORIGIN      
        1 tcctccgcga catctcgctc agcttcttcc ccggcgccaa gatcggcgtg ctcgggttga
       61 acggctccgg caagtccacc ctgctgcgca tcatggcggg cgtggacaag gagttcgagg
      121 gtgaggcgcg cccgcagccc ggcatcaaga tcggcttcct gtcgcaggag ccgcagctcg
      181 acccggccat ggacgtgcgc ggcaacgtcg aggtcgcggt cgccgggatc aaggaagcgc
      241 tcgcgaagct cgacgcggtc tatgccgcct acgccgagcc ggatgctgac ttcgacagga
      301 tcgccgccga gcaggcccgc ctcgaagcga tcatccaggc cgccgatggc cacaacctcg
      361 accgccagct cgagatcgcg gccgacgcgc tgcgcctgcc gccgtgggac gcggacgtca
      421 cgaagatctc gggtggcgag cggcgccggg tcgcgctctg ccagctgctg ctctcgaagc
      481 cggacatgct gctgctcgac gagcccacca accacctcga tgccgagtcg gtgcgctggc
      541 tcgagcacta cctggagcag tacccgggca cggtgatcgc cgtcacccac gaccgctact
      601 tcctcgacaa cgtcgccggc tggatcctcg agctcgaccg gggcttcggc atcccgtggc
      661 agggcaacta ttcctcgtgg ctcgagcaga aggagaagcg cctgcaggtg gaggagcagc
      721 agcaggaagc gctgaagaag atgctggccc gcgagctcga gtgggtgcgc cagaatccga
      781 aggcgcgcca ggccaagagc aaggcccgca tgcagcgctt cgaggaactg cagtcacgcg
      841 agttccagga gcgcaacgag accaacgaga tctacatccc gcccgggccg cgtctgggcg
      901 agctcgtgat cgaggccaag ggcctgtcga aggcgtacgg cgagaagatc ctgttcgaga
      961 acctgaactt caacctgccg cagggcggca tcgtcggcat catcggcccg aacggcgcag
     1021 gcaagacgac gctgttccgc atcctcacgg gccaggagca ggcggacggg ggcgagatcc
     1081 gcatcggccc caccgtgcag ctcgcctacg tggaccagtc gcgccagtcg ctcgacgaca
     1141 agaagaccgt ctggcaggag atctcgaacg gcctcgacat gctcaaggtc ggcaactacg
     1201 agacgccctc ccgcgcctac gtcggccgct tcaacttccg cggctcccag cagcagcagt
     1261 tgatcggcga gctgtccggc ggcgagcgca atcgcgtaca tctcgcgaag gtgctgaagt
     1321 ctggcggcaa cgtcgtgctg ctcgacgaac ccaccaacga cctcgacgtc gagacgctgc
     1381 gcgcgctcga agaggcgctg ctgagcttcc ccggctgtgc ggtcgtgatc tcgcacgacc
     1441 gctggttcct cgaccgcatc gcgacccaca tcctcgcctt cgagggcaac tccgaggtcg
     1501 tctggttcga aggcaactac tccgagtacg tcgaggacct gaaacgccgc cgtggcgagg
     1561 acgccgagca gccgcaccgg atgcgttacc gcagcccgca cgcctgaggc aggtaaactg
     1621 gcgcgggagg atgccatggc cagaagcaag acccggtccg cgctcgagat cgacggcaag
     1681 gagctgacgc tcgaccggtt gcgggcattc gagtcgcggc gcccccgcgt gacgctcgcc
     1741 gccgcggcgc gcaagcgcat gcagggatca cgcgacgccg tgaggcgcgc catcgatcgc
     1801 ggcgacgtgt cgtacggcat caacaccggc ttcggcgcct tcgcgaacaa ggtgatcccg
     1861 gcggccaaca cccgcaagtt gcagttgaac ctgatccgca gccacgcatg cggcaccgga
     1921 cagccgctgc ctgagccgat cgtgcgccgc atgctgctgc tcaaggcgca cagcctcgcc
     1981 gccggccatt ccggggcgcg cccgctggcg gccgagatgc tgctggcgct cctgaactcc
     2041 gacgtgctgc cgatcgtgcc gtcgcaaggc tcggtcggcg cctcgggcga cctcgcgccg
     2101 ctcgcgcacg tcgcgctcgc actcgccggc gagggcgagg ccatgcacga tggcaagcgg
     2161 ctttccggcc gggaggtcct cgacgccgtc ggcctcgagc cgctgggact ggaagcgaag
     2221 gaaggactgg cgctgctcaa cggcacgcag ctgtccctcg cgctcgcgct cgaagggctg
     2281 ttccaggccg agcacctgct ggacgcctcg atcgtcgccg gcgcgctcac ggtcgagggc
     2341 ctggcgggga gccacgtccc cttcgacgcc cgcatccagg aagccagccg gctgccgggc
     2401 caggtcaagg tcggcgagcg gttccgtcgc ctgctcaccg agagcgagat ccgccgcagt
     2461 cacgcggact gcgagcgcgt gcaggacccc tatgcggtgc gctgcatgcc gcaggtgttc
     2521 ggtgccgtcg agcacacgct cgagcatgcc cgcgcggtgc tcggcgcggc ggtgaacggc
     2581 gtgtcggaca atccgctcat cttcggcgac gacatcctgt cgggcggcaa tttccacgcc
     2641 gagccggtcg gattcgtcag cgacttcctc gccatcgcgg tcgccgagct ggcgagcatc
     2701 gcggagcgac gcatcgacct gctcgaccgc cgcgtgaacc cgaacctcaa catgttcatg
     2761 gccgccgagc cgggcctcga gtccggtttc atgatggcgc acgtgaccgc cgcggcgctc
     2821 gtctccgaga acaagacgta cgcgcacccc gcgtccgtgg acagcctgcc gacgtccgcg
     2881 ggccaggaag accacgtgag catggcgccg tgggccgggc gcaagctcct tgcggtctgc
     2941 gccaacacgg cgaccgtgct gggcatcgaa ctgctcgccg cggcgcaggc catcgacgcg
     3001 atgcggcccc tgaccacgac cccggaactg cagcgcgtgc acgcgctggt gcgcgagcgc
     3061 gtgccgtacc gcgcccacga ccaccggctc gaccgcgaca tcgaggccct ggccgcgctg
     3121 gtgcgcgagg gcaccctgtc gtcgttcatc gcgtaacgag cgtgcgcggc gtcgccgtta
     3181 ccctcgccgg attcgcgttc atcgcgctgc tcctcgcctg gagccgctgg ctcacgcggc
     3241 accggctggc agccgtggcg aacctcgcgc tggcgacgat cgccgcgatc gtggcggccg
     3301 ccttgtggtc ggttgccacc ggcctggagg actacgaacc gctgcgcgcg gaaacggtgg
     3361 tcgccgaagt gcgcttcgac gagaccggga cagaccggta ccgggcgacc ttcatgcacc
     3421 tgcccgaagg cggagtccag gtcttcgaga tgcccggcaa ccgctggcgg cttgcggcca
     3481 ggacgctcga gtggcacggg ttcgccgcgg acgccggcct gaagccaatg ttccgtttcg
     3541 aggccctcga ggcggggacg gcaacgaccg acggcgaggg cgtccgggtc ctgcggacct
     3601 accagctggc cgagccggat tcgctcgacg tttgggcgtg ggtccgggcc agccgggcgt
     3661 ggtcgcgctg cgcggtcgcc ggccgggcgg acggtgcgtg gctcccagtg tcccgggggg
     3721 agcacttcgt cgtgcggatt gccgacggcg tcctgcgcat cgagccagcg gcggggccga
     3781 caccgacgct cgcggcaaac cgctgagcgg gtgccggtcg acgcgggttc ggctacactc
     3841 gaagccggat tcccgagcgg gaaggggtga catgtcagtc cacgtcgact cgagcggccg
     3901 cctccaccac gaccaggagc gcagcctcgc gctcaactgc ccgcactgcc aggtcttttc
     3961 gcacatcacg gcagtgtcgg tgccctcgta ctccgagctc gtcgccaacc ggccgagcca
     4021 ggtgggcatc gtctatcgct gcgactcgtg caacgcgccg atcttcctgc gtttcgcggt
     4081 caagatgtac gcggcgaacc gcgtcgagct cgccaacaac ttcgtggagc tcgagcgcgc
     4141 ccgggagaag ttcaacttca cctacctgcc cgaggagagc gagccggtct tccgcgaggc
     4201 gctcggctgc tactcggcgg ggtatttcaa cgcgttcgcg tcgatgtgcc gacgcacggc
     4261 ccaggtggtg ttcgccgacc tcggcgagaa cgggcgcctc aggatcttca accagatcaa
     4321 cgaggcgcgc gagatggcgg agatcgacgg cgacacgttc gccgtcgtga agaaggtgct
     4381 gttcgggacg gatgccgacg cgccgccgtc gttgcccgag atcggcgcct acgaggcggg
     4441 cgtgctgctc gagctcgtga aggacatgct gtaccaggcg tacgtgcgca agggcaagct
     4501 gcagcaggcg atgatggtgc gccgattctt cgcggaagag acgctcgaca agatcacgcc
     4561 gattggccgc gccgccggcg cggactgacg cagctcgcgg agccaactgc gtcagcgcgt
     4621 gacggcctgt tgggtagggg tgcctatccg cggggcgtcg cgcggcatgg acgccgcgcg
     4681 cgagcctcca tggacggatt cacggcgtcc ccgcggatag gcacccctgc cccacaggcc
     4741 tggacgccga cgcgaaacga tccgcgtcgc tgccgctcag ccaacccagg cgcgggcgtt
     4801 gcggaacatc cgcatccacc cactgtcctc gccccact
//
LOCUS       DAOMFD010000152         4831 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7392_length_4831_cov_24.764657, whole genome shotgun sequence.
ACCESSION   DAOMFD010000152 DAOMFD010000000
VERSION     DAOMFD010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4831)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7392_length_4831_cov_24.764657"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PL152_08490"
     CDS             <1..903
                     /locus_tag="PL152_08490"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQR49361.1"
                     /translation="RTSAEHARRHKPLQEEIHVKVERLTCALGAELKGVKLADASRDD
                     GLFADIEHLLLEHKVLFARDQDMTDDEHASFARKFGPLEDHPLAPSAKGEPGIIHIWK
                     TPESPPERYENSWHNDATWRECPPMGAVLRCVECPPVGGDTMWANMELAYERLPQHIK
                     DTIADLRARHSMEASFMAAKPIEERLAMKARFPDPEHPVVRTHPVTGRKSLFTSSFIT
                     HFTNFHTAERVRFGQDLHPGAYNLMEYLVSQSAIPEYQVRWRWTPNAVAIWDNRCTQH
                     YAVMDYAPCHRKMNRATIKGDRPF"
     gene            933..1649
                     /locus_tag="PL152_08495"
     CDS             933..1649
                     /locus_tag="PL152_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HQR49362.1"
                     /translation="MITLYTAATPNGYKASIMLEELALPYHLRVLDLGQQEQKQDWYL
                     EINPNGRIPAIVDHDANDFAVFESGAILMYLAEKTGRLLPTEPKARSRVIQWLMFQMG
                     GVGPMMGQANVFYRYLPEKIPLAIDRYQGEVRRLFGVLDKRLAQHEYLADDYSIADVA
                     NWPWVYTHAWSGIALDEFTHLKRWLAQIRTRPAVIRGLQHPPLKMKLDVSSEENQQAA
                     DQFVAGARAILQTGRASQGA"
     gene            1652..2110
                     /locus_tag="PL152_08500"
     CDS             1652..2110
                     /locus_tag="PL152_08500"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQR49363.1"
                     /translation="MNVTGACHCGAIHFEAEVDESLVFTCHCTDCQIIASTAFRFGAI
                     VRRETFSMRGPYKEYAKIGTSGQRRQQVFCPECATGIYSYSPDSPAPYLSLRLGGVHQ
                     RAQLTPAHQLWYRSALPWAAKLNDIPRCKEQEILVSMLRLAQREPETATG"
     gene            2234..3073
                     /locus_tag="PL152_08505"
     CDS             2234..3073
                     /locus_tag="PL152_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR49364.1"
                     /translation="MKLETRYPQKRVLITGANSGFGKAMAMHFGRNGWRVAVTGRRPD
                     AVRAAAEDVRKAGAAEALELTIDVAKPEDFAQAVERIEAQWGGLDILVNNAGIIDSAR
                     LQDITLEQWHRIMDINLWGVIHGCHAFIPMFIRQQSGYVLNMASSAGLFSLPEMAHYS
                     VPKAAVIALSETMRAELARYGIGVTVSCPAAFASDILNKEGVDTGRSVTLRALDKDAQ
                     SGKHTSESVAAHAIRNMERGRLYDIAHDSMELLWFLKRLMPEAIYHLFAWMYANRKWK
                     FKH"
     gene            3092..4513
                     /gene="gorA"
                     /locus_tag="PL152_08510"
     CDS             3092..4513
                     /gene="gorA"
                     /locus_tag="PL152_08510"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HQR49365.1"
                     /translation="MFTFDADLLVIGGGSGGVRAARMAAARGARVVLAEAAGAAGLGG
                     TCVNVGCIPKKLYSYAAHYAEAFEESRGFGWQPTKPQFDWSSLKRNRRAEIGRLNSVY
                     QKLLDGSGVQTLFGRARFLDAHTVQVDCDRNGERTCRCISAPHILVATGGRPDVPDFP
                     GREHTVTSNEMFDLDPFPSRLVVVGGGYVACEFASIFNGLGSRVTQIYRGPQVLRGFD
                     DDIRAFLGAELVKKGVDLRLNARVSRIEKAIDGLHVTLQDGHHVTADVILCATGRVPN
                     VEGLGLDRAGVAQGPDGAVLVDDHYRTNVPSIYAIGDVTARVQLTPVALGEAMAVVDR
                     LFAPPDGKAPRTMDYELIPTAVFTHPSVGTVGMTEAEARKAHGAIEVYRSDFKALRHT
                     LSGSSERTLMKLVVDAATDRVVGLHMVGADAGEIVQGFAVAMRSGATKADFDRTVGIH
                     PTAAEEFVTMREPVRRDGPKSIS"
     gene            complement(4577..>4831)
                     /locus_tag="PL152_08515"
     CDS             complement(4577..>4831)
                     /locus_tag="PL152_08515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49366.1"
                     /translation="AIGEFLRSIGDDLHYELLPYHRYGESKYGFLGMEVPMTKVVPTA
                     EEQAIHAELARYRARERATVPLSPEQGAPLLERVRSGHAS"
BASE COUNT          878 a         1592 c         1613 g          748 t
ORIGIN      
        1 cgcaccagtg cagagcatgc gcgccgccac aaaccattgc aggaggagat acacgtgaag
       61 gtcgaacgac tgacgtgcgc cctgggcgcg gaactgaagg gcgtgaagct ggccgatgcg
      121 tcgcgggacg acggcctgtt tgccgacatc gagcatttgc tgctcgaaca caaggtgctc
      181 ttcgcgcgcg accaggacat gaccgacgac gagcatgcca gcttcgcacg caagttcggt
      241 ccgctggagg accacccgct cgcccccagc gcgaagggcg agccgggcat catccacatc
      301 tggaagacgc cggagagccc gccggagcgc tacgagaact cctggcacaa cgatgccacc
      361 tggcgcgaat gcccgccaat gggcgcggtg ctgcgctgcg tcgagtgccc gcccgtgggc
      421 ggcgacacga tgtgggccaa catggagctg gcctacgagc ggctgccgca gcacatcaag
      481 gacaccattg cggacctgcg tgcgcgccac agcatggaag cctcgttcat ggcggcgaag
      541 cctatcgagg agcggctggc gatgaaggcg cgcttccccg atcccgagca tccggtcgtg
      601 cgcacgcacc cggtgaccgg caggaagagc ctctttacga gctcgttcat cacgcacttc
      661 accaacttcc acacggccga gcgggtgcgt ttcggccagg acttgcatcc cggtgcgtac
      721 aacctgatgg agtacctggt cagccagtcc gcgattcccg agtaccaggt gcgctggcgc
      781 tggacgccga acgcggtggc gatctgggac aaccgctgca cgcagcacta cgcggtcatg
      841 gattacgcgc catgccaccg caagatgaat cgcgccacga tcaagggcga ccgcccgttc
      901 tgaccgatat cactacaggt gccctagacg ccatgattac gctctacacc gctgcgacgc
      961 cgaacggcta caaggcctcc atcatgctgg aggaactggc gctgccgtac cacctgcggg
     1021 tgctggacct cggccagcag gaacagaagc aggactggta cctcgagatc aacccgaacg
     1081 gccgcatccc ggccatcgtc gaccacgacg cgaatgattt cgcggtcttc gagtcgggtg
     1141 cgatcctgat gtacctggcc gagaagaccg ggcggctgct gccgacggag cccaaggccc
     1201 gctcgcgcgt catccagtgg ctgatgttcc agatgggcgg ggtcggcccg atgatgggac
     1261 aggccaacgt gttctaccgc tacctgccgg agaagatccc gctcgcgatc gaccgctacc
     1321 agggcgaggt gcgccgactc ttcggcgtgc tcgacaagcg actggcgcag cacgagtacc
     1381 tggcggacga ctattccatc gcggacgtcg ccaactggcc gtgggtctac acccatgcct
     1441 ggtccgggat cgcgctggat gagttcacgc acctcaagcg gtggctcgcg cagatccgga
     1501 cccgaccggc cgtgatccgg ggcctgcagc acccaccgct gaagatgaaa ctcgacgtat
     1561 ccagcgaaga gaaccagcag gctgccgacc agttcgtggc tggcgcccgc gccatccttc
     1621 agaccggccg cgccagccag ggagcctgac catgaacgtt accggcgctt gccattgcgg
     1681 cgcgattcac ttcgaggccg aggtcgacga gagcctggtg ttcacctgtc actgcaccga
     1741 ctgccagatc atcgcctcga cggccttccg tttcggggcg atcgtgcggc gcgagacgtt
     1801 ctcgatgcga ggcccataca aggagtacgc caagatcggc accagcggcc agcgtcgcca
     1861 gcaggtgttc tgcccggaat gcgccaccgg gatctactcg tactcgcccg acagtcccgc
     1921 gccgtattta agcctgcgcc tgggaggcgt ccaccagcgg gcgcagctga cgccggcgca
     1981 ccagctctgg taccgctcgg cgctgccgtg ggcagcgaag ctcaacgaca tcccccggtg
     2041 caaggagcag gagattcttg tgtccatgct ccggctggcg cagcgcgagc cggagacggc
     2101 aaccggctga cgtggcatct gaatgcgggc gtgcagcccg caatcgcgga tggtagcttg
     2161 gtaatgaaag gcccgcgtgc catgcacgcg agccgactgc gccgcgaaag tcggcgacga
     2221 gggggcgaac tcaatgaagc tcgagacgag atatccgcag aagcgggtgc tgatcacggg
     2281 tgccaacagc ggcttcggca aggcgatggc catgcacttc ggccgtaacg gctggcgcgt
     2341 cgcggtcacc ggacgccggc cggatgccgt cagggcggcg gccgaggacg tgcgcaaggc
     2401 gggcgccgcc gaggccctcg aactcaccat cgacgtcgcg aagccggaag attttgccca
     2461 agcggtcgag cgcatcgagg cgcagtgggg cggcctcgac atcctggtga acaacgccgg
     2521 catcatcgac tcggccaggc tccaggacat caccctcgag cagtggcacc gcatcatgga
     2581 catcaacctg tggggcgtca tccacggctg ccatgcgttc atcccgatgt tcatacggca
     2641 gcagagcggc tatgtgctga acatggcctc ctcggcgggc ctgttttctc tgccggaaat
     2701 ggcgcattac agcgttccca aggcggccgt gatcgcactg tcggaaacga tgagggcgga
     2761 gctggcccgc tacgggatcg gcgtgaccgt ctcctgtccc gcggcattcg cctccgacat
     2821 cctgaacaag gaaggcgtgg acaccggccg gagcgtgacg ctccgcgcgc tcgacaagga
     2881 tgcgcagtcg gggaagcaca cgtcggagag cgtggcggcc cacgccatcc gcaacatgga
     2941 gcgcggccgt ctctacgaca tcgcccacga ctccatggaa ctcctgtggt tcctgaagcg
     3001 actgatgccc gaggccatct accacctctt cgcgtggatg tacgcgaacc ggaaatggaa
     3061 gttcaagcat tgaccgcatc cgggacgcgc cgtgttcacg ttcgacgctg acctgctggt
     3121 catcggcgga ggcagcggcg gcgtccgggc ggcgcggatg gcggctgcgc gcggtgcgcg
     3181 agtcgtgctc gcggaagcgg ccggcgcggc cggactgggc ggaacctgcg tcaacgtcgg
     3241 ctgcatcccg aagaagctct acagctacgc ggcccactac gcggaggcgt tcgaggagtc
     3301 ccgcggcttc gggtggcagc cgaccaagcc gcaattcgac tggtccagcc tcaagcggaa
     3361 tcgccgcgcc gagatcgggc gcctgaattc cgtctatcag aagctgctcg acggcagcgg
     3421 cgtccagacg ctgttcggcc gcgcgcggtt cctggatgcg cacacagtcc aggtcgactg
     3481 tgaccgcaac ggcgaacgca cctgcaggtg catcagcgca ccgcacatcc tcgtcgcgac
     3541 cggcgggcgt ccggacgtcc cggactttcc gggtcgcgag cacaccgtta cgtcgaatga
     3601 gatgttcgac ctcgatccgt tcccgtcccg gctggtcgtg gtggggggcg gctacgtcgc
     3661 gtgcgagttc gcgtccatct tcaacgggct gggttccagg gtgacccaga tttatcgtgg
     3721 tccccaggtc ctgcgcgggt tcgacgacga catccgcgcc ttcctcggcg ccgagctggt
     3781 caagaaaggg gtcgacctgc gactgaacgc gcgggtatcg cgaatcgaga aggccatcga
     3841 tggactgcac gtgacactgc aggacggcca ccacgtgacg gccgacgtga tcttgtgcgc
     3901 aaccggtcgc gtgccgaatg tcgagggcct cggactggat cgcgccggtg tcgcacaggg
     3961 gccggacggc gcggtcctcg tcgacgacca ctaccggacc aacgtgccgt ccatctacgc
     4021 catcggtgac gtcactgcac gagtgcagct gaccccagtc gccttgggag aggccatggc
     4081 tgtcgtcgat cgattgttcg caccgccgga cggcaaggcg ccacgcacca tggactacga
     4141 actgatcccg acggcggtgt tcacgcaccc cagcgtcggc accgtgggca tgaccgaagc
     4201 cgaggcacgc aaggcgcatg gcgcaatcga ggtctaccgc agcgatttca aggccctgag
     4261 gcacaccctg agcggcagca gcgagcgtac gctgatgaag ctggtggtcg acgccgcgac
     4321 ggatcgagtc gtcgggctgc acatggtcgg tgccgacgcg ggggagatcg tgcagggctt
     4381 tgcggtggcg atgcggtccg gtgcaaccaa ggccgatttc gatcgaaccg tcggcatcca
     4441 ccccaccgcg gccgaggagt tcgtcaccat gcgcgagcct gtccggcgcg atggtccgaa
     4501 gtcgatctcg tgacaccgac ttcgcagggg ggcgctgggg actgaaggcg cggcggtctg
     4561 ggcgggaacg agcgcgtcag cttgcgtggc ccgaacgcac gcgttcgagc agcggcgcgc
     4621 cctgctccgg agacagcggc acggtcgccc gctcgcgcgc acggtagcgg gcaagttcgg
     4681 cgtggatggc ctgctcctcc gccgtcggca cgaccttcgt catgggcact tccatgccga
     4741 ggaaaccgta cttcgactcg ccgtagcggt gatacggcag caactcgtaa tgcaggtcgt
     4801 cgccgatcga gcgcaggaac tcaccgatcg c
//
LOCUS       DAOMFD010000153         4817 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7417_length_4817_cov_34.565939, whole genome shotgun sequence.
ACCESSION   DAOMFD010000153 DAOMFD010000000
VERSION     DAOMFD010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4817)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4817
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7417_length_4817_cov_34.565939"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1571)
                     /gene="leuS"
                     /locus_tag="PL152_08520"
     CDS             complement(<1..1571)
                     /gene="leuS"
                     /locus_tag="PL152_08520"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HQR49367.1"
                     /translation="MQERYEPAVVESEAQRHWDSARTFAVTEDASRPKYYCLCMFPYP
                     SGRLHMGHVRNYTIGDLLTRYMRMQGYNVLQPMGWDAFGLPAENAAMANGVPPAKWTY
                     DNIAYMKRQLKSLGFGIDWDREVATCRPEYYRWNQWLFLRMLEQGIAYRTTGTVNWDP
                     VDKTVLANEQVIDGRGWRTGAVVEKREIPMYYLRITQYAEELLGALESLPGWPERVKL
                     MQSNWIGRSEGLELEFEVSGVAEPLRIYTTRPDTLYGVTYMAVAAEHPLAQRAAAGNP
                     DLQAFIAECQRTGVTEAVLETMEKRGMPTGIDAIHPLTGERVPVWVANFVLMGYGTGA
                     VMAVPAHDQRDWEFARRYGLRIHQVIQPADGGLVDLEAGAFVEHGRLINSGRFDGLDF
                     DEAFDAIAKSFEAGGRGRRRVNWRLRDWGISRQRYWGCPIPLIHCATCGDVPVPDEQL
                     PVVLPEDCVPDGSGNPLNRNEAFLACTCPKCGKPARRETDTMDTFVDSSWYFFRYASP
                     GNDRAMVDDRAAYWMP"
     gene            complement(1630..2703)
                     /locus_tag="PL152_08525"
     CDS             complement(1630..2703)
                     /locus_tag="PL152_08525"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR49368.1"
                     /translation="MSEQEDAITPEGERYASPPSELDYLDVDPPQPGHAVAIADGLLW
                     ARIPLPMELAHINVWLLEDADGWTLVDTGMAAEPCREAWVTLEREVLGRRGLRRILVT
                     HDHPDHMGLSPWLQRKHGAEVWMSAIAHQSSAEFLATESSRIYARMATFLAMHGMPVD
                     AATAQRPMPGHDTWFDGVPELTRATAGGDALGTPGRTWDVVETSGHCRGHLCLHDAAG
                     RLLVSGDQVLPGISPNVSVLSSRPDADPLREFLASLTRLEQCAGDTLVLPSHGRPFRG
                     LHHRTASLRAHHEQQLGGLREACAEARTAFELLPAMFGRPLRGFHRVLAMGETVAHLN
                     YLWSAGLVERSVDTGGVIRFVRA"
     gene            complement(2700..4223)
                     /gene="lnt"
                     /locus_tag="PL152_08530"
     CDS             complement(2700..4223)
                     /gene="lnt"
                     /locus_tag="PL152_08530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HQR49369.1"
                     /translation="MLRPVASVVAFVDRWGHWIALPVGALLALAFAPTSAWPLAFACP
                     AVLMLLWQGAAPRSAAWRGFLFTGGTFLAGTYWLYHSIHLIGKAPIWLALFLMLGMSA
                     IMGSYTALLGYAAARWGPERGPLRWLVLVPAGWVLVEWLRGWFLSGFPWLALAYTQLD
                     TVLAAYAPVGGAYAVSLATVVLAGALVTFLLGKRADRMWAAATVIVVCVGGGVLSRIE
                     WTQPRGTSVTVALVQGAVPQSMKWVPGELERTMDLYVELTRPYLGADIIVWPESAIPA
                     LEGNIRDFLDGVTALARSRGSSLVTGLVRRDPATGAYYNGIAAWAPGEQWYYKRHLVP
                     FGEFFPVPAAVRDWMRLMSLPYSDFSAGADAQDPLVAGGERLAPTICYEDAYGVEQLP
                     YARRATLLVNVTNDAWFGDSSAPHQHLDISRMRSLETGRPMLRATNDGVTALIAGDGK
                     LIGILPQFRPGVLTGRIEPRTGLTPYMRSGNWPLLGLLWLGLVAGFGWRWLRSRPVR"
     gene            complement(4256..>4817)
                     /locus_tag="PL152_08535"
     CDS             complement(4256..>4817)
                     /locus_tag="PL152_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HQR49370.1"
                     /translation="EDRDEVAGILLAKDLLRYVAQGEPAQFDIRECLRPAVLIPESKR
                     LNVLLREFRVSHNHMAIVVDEYGGVSGLLTIEDVLEEIVGEIGDEYDVDEGEGIRKEG
                     ERTFTVPALTRIDEFNRVFGTRFADEEFDTIGGLVLHELGRLPRRGESIEIGGLDLQV
                     LRADRRRIEALRVRTQRDFELKPGPL"
BASE COUNT          729 a         1721 c         1666 g          701 t
ORIGIN      
        1 ggcatccagt acgcggcgcg gtcgtccacc atcgcccggt cgttgcccgg actcgcatag
       61 cggaagaaat accacgacga gtccacgaag gtgtccatcg tgtccgtttc gcggcgcgcc
      121 ggcttgccgc acttcgggca ggtgcaggcg aggaacgcct cgttgcggtt gagcggattg
      181 ccgctgccgt cgggcacgca gtcctcgggc agcacgaccg gcaactgctc gtccggcacg
      241 gggacgtcgc cgcacgtcgc gcagtggatg agcgggatcg ggcagcccca gtagcgctgg
      301 cgcgagatgc cccagtcgcg cagccgccag ttcacgcggc ggcggccgcg gccgcccgcc
      361 tcgaaggact tcgcgatcgc gtcgaaggcc tcgtcgaagt cgaggccgtc gaagcggccc
      421 gagttgatga gtcgtccgtg ctccacgaac gcgccggctt ccaggtcgac gaggccgccg
      481 tccgcgggct ggatcacctg gtggatgcgc aggccgtagc ggcgcgcgaa ttcccagtcg
      541 cgctggtcgt gcgccgggac tgccatcacg gcgccggtgc cgtagcccat cagcacgaag
      601 ttcgccaccc acaccgggac gcgctcgccg gtgagcggat ggatcgcgtc gatgcctgtc
      661 ggcatcccgc gcttctccat cgtctcgagc accgcctccg tcaccccggt cctctggcac
      721 tcggcgatga aggcctgcag gtcgggattg cccgcggccg cgcgctgcgc gagcgggtgc
      781 tcggcggcga ccgccatgta ggtgacgccg tagagcgtgt cgggccgggt cgtgtagatc
      841 cgcaggggct cggccacgcc gctgacttcg aactcgagct cgaggccctc tgagcggccg
      901 atccagttcg actgcatcag cttcacgcgc tccggccagc cgggcagcga ctcgagcgcg
      961 ccgagcagtt cctccgcgta ctgcgtgatg cggaggtagt acatcgggat ctcgcgcttc
     1021 tcgacgaccg cgccggtgcg ccagccgcgc ccgtcgatga cctgctcgtt cgcgaggacc
     1081 gtcttgtcca cggggtccca gttgaccgtg cccgtggtgc ggtaggcgat accctgctcc
     1141 agcatgcgca ggaacagcca ctggttccag cggtagtact ctggccggca ggtggcgacc
     1201 tcccggtccc agtcgatgcc gaagcccagc gacttcagct ggcgcttcat gtaggcgatg
     1261 ttgtcgtagg tccacttcgc gggcgggacg ccgttcgcca tcgccgcgtt ctccgccggc
     1321 aggccgaacg cgtcccagcc catgggctgc agcacgttgt agccctgcat gcgcatgtag
     1381 cgcgtgagca agtcgccgat ggtgtagttg cgaacgtgtc ccatgtgcag ccggccgctc
     1441 gggtacggga acatgcacag gcagtagtac ttcggacgcg aagcgtcttc cgtcaccgcg
     1501 aacgtgcgcg cggaatccca gtggcgctgg gcctcggact cgacgacggc gggttcgtag
     1561 cgttcttgca tgtcgcgcgg gcgggtcggg agcgggccga aaaggatacc agacggggcg
     1621 gcggccgatt caggcgcgga cgaaccggat caccccgccg gtgtcgacac tgcgttcgac
     1681 caggcccgcc gaccacaggt agttcaggtg cgccacggtc tcccccatcg ccagcacgcg
     1741 atggaagccg cggagcgggc gcccgaacat cgccggcagc aattcgaagg cggtccgcgc
     1801 ctccgcgcac gcctcgcgca gcccgccgag ctgctgctcg tggtgcgcgc gcaggctcgc
     1861 ggtgcggtgg tgcagcccgc ggaacgggcg cccgtgagac ggcagcacca gcgtgtcccc
     1921 ggcacactgc tcgagtcgcg tgagcgaggc gaggaactcc cggagcggat ccgcgtcggg
     1981 ccggctcgag agcacgctca cgttcggcga gatgcccggc aggacctggt cgccgctcac
     2041 gagcagccgc cccgcggcgt cgtgcaggca aaggtggcca cgacagtgcc cgctcgtctc
     2101 gaccacgtcc caggtgcgtc ccggcgtgcc cagcgcgtcg cctccggccg tcgcgcgcgt
     2161 gagttcgggg acaccgtcga accacgtgtc gtgacccggc atcggccgtt gcgccgttgc
     2221 agcgtcgacc ggcatgccgt gcatggcgag gaacgtggcc atgcgcgcgt agatgcggct
     2281 cgactcggtg gcgaggaatt ccgcggacga ctggtgcgcg atcgccgaca tccagacctc
     2341 ggcgccgtgc ttgcgctgca gccacggcga caggcccatg tggtcggggt ggtcgtgcgt
     2401 gaccaggatc cgccgcaggc cgcggcgtcc cagcacctcg cgctcgagcg tcacccaggc
     2461 ttcgcggcat ggttcggccg ccatcccggt gtccacgagc gtccagccat ccgcgtcctc
     2521 gagcaaccag acattgatgt gcgcgagttc catcggcagc gggatgcgcg cccagagcaa
     2581 gccgtcggcg atcgccaccg cgtgtcccgg ctgcggcggg tcgacgtcga gatagtcgag
     2641 ttcgctgggc ggcgacgcgt agcgctcgcc ttccggcgtg atcgcgtcct cctgctcgct
     2701 catcgcacgg gcctgctgcg cagccagcgc cagccgaagc ccgcgaccag cccgagccac
     2761 agaaggccga gcagcggcca gttgccgctg cgcatgtagg gcgtgaggcc ggtccgcggt
     2821 tcgatgcgac cggtgagcac tccgggccgg aattgcggca ggatcccgat cagcttgccg
     2881 tcgccggcga tgagcgccgt cacgccgtcg ttggtcgcgc gcagcatcgg ccgcccggtc
     2941 tcgagcgagc gcatgcggct gatgtcgagg tgctggtggg gcgccgagga gtcgccgaac
     3001 cacgcgtcgt tggtgacgtt gacgagcagc gtcgcgcggc gcgcgtacgg caactgctcg
     3061 accccgtagg cgtcctcgta gcagatggtg ggcgccagcc gctcgcctcc ggcgaccagc
     3121 gggtcctgcg cgtccgcgcc tgccgagaaa tccgagtacg gcaggctcat caggcgcatc
     3181 cagtcgcgca cggccgcggg caccgggaag aactccccga atggcacgag gtgtcgcttg
     3241 taataccact gttcccccgg cgcccacgcg gcgatgccgt tgtagtaggc gccggtcgcc
     3301 ggatcgcgcc ggacgagccc ggtgacgagc gacgacccgc gcgacctcgc gagcgccgtc
     3361 acgccatcga ggaagtcccg gatgttgccc tcgagcgcag ggatggccga ctccggccac
     3421 acgatgatgt cagccccgag atacggcctc gtcagttcaa catacaggtc catcgtgcgc
     3481 tcgagctctc ccggcaccca cttcatcgac tgcggcacgg cgccctgcac cagcgcgacc
     3541 gtcaccgacg tgccgcgcgg ctgcgtccac tcgattcgac tcaggacgcc gccgccgacg
     3601 cacacgacga tcaccgtcgc ggcggcccac atgcggtccg cacgcttccc gagcaggaag
     3661 gtcacgagcg cgccggcgag cacgacggtc gcgaggctca ccgcgtacgc gccgccgacg
     3721 ggcgcgtatg cggccagcac ggtgtcgagc tgggtgtatg cgagcgcgag ccaggggaat
     3781 ccgctgagga accagccacg tagccattcc accaggaccc agcctgccgg caccaggacg
     3841 agccagcgca gcggtccgcg ctccgggccc cagcgcgccg ctgcgtagcc gagcagcgcc
     3901 gtgtagctgc ccatgatcgc ggacatgccg agcatcagga agagcgcgag ccagatcggc
     3961 gctttcccga tcaggtggat gctgtgatag agccagtagg tgccggcaag gaacgtcccg
     4021 ccggtgaaca ggaaaccacg ccaggccgcg ctccgcggcg cggcgccctg ccacagcagc
     4081 atgagcaccg cgggacatgc gaacgccagc ggccaggcgg acgtcggcgc gaacgcgagc
     4141 gcgagcagcg cccccacagg cagtgcgatc cagtgccccc atcggtccac gaacgcgacg
     4201 acgctcgcca ccggacggag caccccggta tcccctcgga acgatccatg cgccactaca
     4261 gcggaccggg cttgagttcg aagtcgcgct gggtgcgcac gcgcagcgcc tcgatcctgc
     4321 ggcggtcggc gcgcagcacc tggaggtcca gcccgccgat ctcgatcgac tcgccgcgcc
     4381 gcggcaggcg cccgagctcg tgcagcacga gccctccgat ggtgtcgaac tcctcgtccg
     4441 cgaagcgcgt gccgaacacc cggttgaact cgtcgatgcg cgtcagcgcc ggcaccgtga
     4501 acgtgcgctc gccttccttg cggatgccct cgccttcgtc cacgtcgtat tcgtcgccga
     4561 tctcgccgac gatctcctcg agcacgtcct cgatcgtgag cagtcccgac acgccgccat
     4621 attcgtcgac gacgatcgcc atgtggttgt ggctcacgcg gaattcgcgc agcagcacgt
     4681 tcaggcgctt gctctccggg atcagcaccg ccgggcgcag gcactcacgg atgtcgaact
     4741 gcgccggctc cccctgggcg acgtagcgca gcaggtcctt ggcgagcagg atgccggcga
     4801 cctcgtcgcg gtcctcg
//
LOCUS       DAOMFD010000154         4816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7420_length_4816_cov_38.911783, whole genome shotgun sequence.
ACCESSION   DAOMFD010000154 DAOMFD010000000
VERSION     DAOMFD010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4816
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7420_length_4816_cov_38.911783"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            314..592
                     /gene="rpoZ"
                     /locus_tag="PL152_08540"
     CDS             314..592
                     /gene="rpoZ"
                     /locus_tag="PL152_08540"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004899123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HQR49371.1"
                     /translation="MARITVEDCLQNVPNLFQLVLVASKRARKLANGAEATLDWQNDK
                     PTVLALREVAAGHVGLEILEEPDHPPQEFVPEPEFMGELRAPHLGLGD"
     gene            614..2767
                     /locus_tag="PL152_08545"
     CDS             614..2767
                     /locus_tag="PL152_08545"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HQR49372.1"
                     /translation="MDVTSSLRSLLPYGRRSIGISQLIAKLEGYLPPAEVERVQDAYD
                     FAYSAHSGQRRRSGEPYITHPVAVADLLADLKLDSQTLIAAILHDVMEDTPTLKDEIK
                     ARFGAEVAELVDGVSKLDQIQFKSREEAQAESFRKMVFAMVRDIRVIMVKLADRTHNM
                     RTLGAMPPAKRRAIARETLDIYAPIANRLGMHAIKVELEDLGFRSLYPQRYRVIETAV
                     RKAKGNQKQFLSRIGEALRAALGKAGIEGRVEGREKDVHSIYQKMRRKKSSLAEIVDV
                     YGVRIVVDSVDMCYRALGVVHGVYKPMPGRFKDYIAIPRANGYQSLHTTLFGPSGTPI
                     EVQIRTREMHEVAESGIAAHWQYKDGDESAHTYSERAREWLQRLVEIQQGGNSEEFLE
                     SVKVDLFPDKVYVFTPKGDILRLPRGSTCVDFGYAVHTDVGNRCVAAKVDRRLVPLRT
                     PLRNGQTVEIITAKGATPNPSWTTFVVTAKARAAIRQYLKGLKRSEAVDLGKRLLNQA
                     LEDFSLSVRKLPPETLATVVREFGLRSEDDLYEKIGLGERVAPFVARRLLPADTDTTP
                     DTHAGPLAIAGTEGLVVTYARCCFPIPNDPILANLSTGRGVVIHRDVCGNLSTFRKQP
                     DKWIPVAWRPAVDQLFHVEIKVEVSNRMGVLAQVAAAIAGTQTNIDRVSLTERDSDSS
                     TLTFELMVQDRRHLARVIRAIRAMPEVLKVTRTLA"
     gene            2883..3263
                     /locus_tag="PL152_08550"
     CDS             2883..3263
                     /locus_tag="PL152_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003754044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HQR49373.1"
                     /translation="MPFTPVHTEHAPGAIGTYSQAVRAGSTVYLSGQIPLDPRTGELV
                     TGDMEAQVRRVFDNLQAVAVAAGGDLSHVVKLTVFLTDLANFALVNRVMAEYFQPPYP
                     ARAAVGVAALPRGAAVEMDAILAL"
     gene            3273..>4816
                     /locus_tag="PL152_08555"
     CDS             3273..>4816
                     /locus_tag="PL152_08555"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF016724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HQR49374.1"
                     /translation="MSSAPAQPPLDERPVTSLRGVGPALASTLARIGIHTVQDLIFHL
                     PLRYEDRTRVVPIGTLRPGDRAVIEGEVQLAEVVFRRRRALLCRLADGSGSVTLRFFH
                     FSAAQQEALARGTRLRCFGEARIGPGGLEIVHPEYRRLTVDAAPPEAEALTPIYPAAE
                     GMSQGRLRLLTSLALAQMADGALRDWVTPDVLVAARMPGLEAALRYVHRPPPNADIEL
                     LNAGRHPAQRRLAFEELLAHQISLKLLRQAADRDDAWALASRTDLVQRCLARLPFALT
                     GAQQRAWNEIAADLAQERPMMRLVQGDVGSGKTVVAALAALRAVENGAQAAVMAPTEL
                     LAEQHARVFLGWLEPLGVRVGLLTGRHGGKVRRSLEQDLASGAIQVAVGTHALFQEGV
                     GFARLGLVIVDEQHRFGVHQRVRLREKGLSDGRYPHQLVMTATPIPRTLAMTAYADLD
                     VSVIDELPPGRMPVRTVVLPESRRDEVVQRIEAACRSGRQAYWVCPLIDESEAVRHQA
                     AEETAAA"
BASE COUNT          750 a         1736 c         1628 g          702 t
ORIGIN      
        1 tgccagcctc aagcgaattt tttcatccat gtcagtgcct tagggttgtg gcgccaggct
       61 ccaggttgcg tctggcacaa cctaccaccg tcgtgcccgg tcgccatgca ctttgccaca
      121 attttgtgac tgtcatcacg cacgccgggc cgctttgccg cggtgccgtc cggcattttc
      181 gccactgtcc gtcagcgggg tcgcacgtga acacgccgcc tgctgcggca tttccggcca
      241 gagatgggct tcggcccggt tcatggtact ctttcttttc ttgtccccaa cctgaccgcc
      301 ggatccacgg accatggccc gcatcaccgt cgaagactgc ctgcagaacg tcccgaacct
      361 gttccagctc gtgctggtcg cctccaagcg cgcccgcaag ctcgcgaacg gcgccgaggc
      421 gaccctcgac tggcagaacg acaagccgac ggtgcttgcg ctccgcgagg tggccgccgg
      481 gcacgtcggc ctcgagatcc tcgaggagcc ggaccacccg ccgcaggagt tcgtccccga
      541 gcccgagttc atgggcgagt tgcgcgcgcc ccacctcggc ctgggagact gatcccgccc
      601 cggccccccg gccatggacg tcacgtcttc cctgcgcagc ctgctgccgt acggaagacg
      661 gtccataggc atcagccagc tgatcgccaa gctcgagggc tacctgcccc cggcggaagt
      721 cgagcgcgtc caggacgcct atgacttcgc gtacagcgcg cactccgggc agcgccgccg
      781 ttccggcgag ccgtacatca cgcatcccgt ggcagtcgcg gacctgctgg ctgacctcaa
      841 gctcgactcg cagacgctga tcgccgcgat cctccacgac gtcatggagg acacgccgac
      901 cctcaaggac gagatcaagg cgcgcttcgg cgccgaggtc gccgagctgg tcgatggcgt
      961 gagcaagctc gaccagatcc agttcaagag ccgcgaggaa gcccaggccg agagcttccg
     1021 caagatggtg ttcgccatgg tgcgcgacat ccgcgtcatc atggtcaagc tcgccgaccg
     1081 cacccacaac atgcgcaccc tgggcgcgat gccgcccgcc aagcgccgcg ccatcgcgcg
     1141 cgagacgctc gacatctacg cgccgatcgc caaccgcctc ggcatgcacg cgatcaaggt
     1201 cgagctcgag gacctcggct tccgctcgct gtacccgcag cgctaccggg tgatcgagac
     1261 ggcggtccgc aaggcgaagg gcaaccagaa gcagttcctg tcgcgcatcg gcgaggcgct
     1321 tcgcgcggcg ctcggcaagg ccgggatcga gggccgcgtc gagggccgcg agaaggacgt
     1381 ccacagcatc taccagaaga tgcgtcgcaa gaagtcgtcg ctcgccgaga tcgtcgacgt
     1441 gtacggcgtg cgcatcgtgg tcgacagcgt cgacatgtgc tatcgcgcgc tcggcgtcgt
     1501 gcacggcgtg tacaagccca tgccgggacg cttcaaggac tacatcgcga ttccgcgcgc
     1561 gaacggctac cagtcgctgc acacgacgct gttcggaccg agcggcacgc cgatcgaggt
     1621 gcagatccgc acccgggaga tgcacgaggt cgcggaatcc ggcatcgccg cgcactggca
     1681 gtacaaggac ggcgacgagt cggcgcacac ctacagcgag cgggcgcgcg agtggctgca
     1741 gcggctggtc gagatccagc agggaggcaa ttccgaggaa ttcctcgaga gcgtcaaggt
     1801 cgacctgttc cccgacaagg tctacgtgtt cacgcccaag ggcgacatcc tgcggctgcc
     1861 gcgcggctcg acctgcgtcg acttcggcta cgccgtgcac accgacgtcg gcaatcgctg
     1921 cgtggcggcc aaggtggacc ggcggctcgt gccgctgcgc acgccgctca ggaacggaca
     1981 gaccgtggag atcatcaccg ccaagggcgc cacgcccaac ccctcgtgga ccacgttcgt
     2041 ggtcacggcc aaggcgcgcg ccgccatccg ccagtacctg aagggactca agcgctcgga
     2101 agcggtggac ctcggcaagc ggctgctgaa ccaggcgctc gaggatttct cgctcagcgt
     2161 gcgcaagctg ccgccggaga cgctggcgac cgtggtccgc gagttcgggc tgcgcagcga
     2221 ggacgacctg tacgagaaga tcggtctcgg cgagcgcgtc gcgccgttcg tcgcgcggcg
     2281 cctgctgccg gcggacacgg acaccacgcc cgacacgcac gcgggcccgc tcgcgatcgc
     2341 cggcaccgag ggcctcgtcg tcacctacgc gcgctgctgc ttcccgatcc cgaacgaccc
     2401 gatcctcgcc aacctgtcca cgggccgcgg cgtggtgatc caccgcgatg tctgcggcaa
     2461 cctgtccacg ttccgcaagc agccggacaa gtggatcccg gtggcgtggc gtcccgccgt
     2521 cgaccagctc ttccacgtcg agatcaaggt cgaggtgtcg aaccgcatgg gcgtgcttgc
     2581 acaggtcgcc gcggcgatcg cgggcacgca gaccaacatc gaccgcgtgt cgctcaccga
     2641 gcgcgacagc gacagctcga cgctcacgtt cgagctgatg gtgcaggacc gcaggcacct
     2701 cgcgcgggtg atccgcgcga tccgcgccat gccggaagtc ctcaaggtga cgcgcaccct
     2761 cgcctgagcg cccggcgggc accccgggac ggggcggggc gcgggcccgc accggacttc
     2821 actaatcccc gatccgtggt catcatccgc catccgactc acccgacccc gcggagccca
     2881 tcgtgccatt cacccccgtc cacaccgaac acgcgccggg cgccatcggc acgtactcgc
     2941 aggccgtgcg cgccggcagc accgtctacc tgtccgggca gataccgctc gatccgcgga
     3001 cgggagagct ggtgacgggc gacatggagg cgcaggtgag gcgcgtgttc gacaacctgc
     3061 aggcggtcgc cgtggcggcc ggcggcgacc tgtcccacgt cgtgaagctc accgtgttcc
     3121 tgaccgacct cgcgaacttc gcgctggtca accgcgtcat ggcggaatac ttccagccgc
     3181 cgtatccggc ccgcgcggcg gtcggcgtgg ctgcgctccc gcgcggcgcc gcagtcgaga
     3241 tggacgcgat actcgcgctg tgacgtgccg ggatgtcgag cgcgccggct cagccaccgc
     3301 tggatgaacg gccggtcacc agcctgcgcg gcgtcggccc ggcgctggcc tccacgctcg
     3361 cgcgcatcgg catccacacg gtccaggacc tgatcttcca tttgccgctg cgctacgagg
     3421 accgcacgcg cgtggtcccg atcggcacgc tgcgtcccgg agaccgcgcg gtgatcgaag
     3481 gcgaggtgca gctcgcggag gtggtcttcc ggcgccggcg cgccctgctg tgccgccttg
     3541 ccgacggctc ggggtccgtg acgctgcgct tctttcattt ctcggcggcg cagcaggaag
     3601 cactcgcgcg cggcacgcga ctgcgctgtt tcggcgaggc gcgcatcggc ccgggcggcc
     3661 tggagatcgt gcacccggag taccgcaggc tcacggtaga cgcggcgccg ccggaggcgg
     3721 aggccctgac gccgatctat ccagcggcgg agggcatgtc gcagggccgg ttgcgcctgc
     3781 tcacgagcct cgcgctcgcg cagatggcgg acggggccct gcgcgactgg gtcacgccgg
     3841 acgtgctggt cgcggcgcgc atgccgggcc tcgaggcagc gctgcgctac gtgcatcgac
     3901 cgccgcccaa cgccgacatc gagttgctga acgctgggcg ccacccggcg cagcgacggc
     3961 tcgccttcga ggaactgctc gcccaccaga tcagcctgaa gctgctgcgc caggcggcgg
     4021 atcgcgacga tgcctgggca ctcgccagcc gcacggacct ggtgcagcgc tgcctcgccc
     4081 gcctgccctt cgcactcacc ggcgcgcagc aacgcgcctg gaacgaaatt gccgccgacc
     4141 tcgcgcagga gcgtccgatg atgcggctcg tgcagggtga cgtcggctcc ggcaagacgg
     4201 tcgtcgcggc gctcgcggcg ctgcgcgccg tcgagaacgg cgcacaggcc gcggtcatgg
     4261 cgccgacgga gctgctggca gaacagcacg cgcgggtgtt cctcggctgg ctcgagccgc
     4321 tgggggtacg cgtcggcctg ctgaccggtc gccacggcgg caaggtccgc aggtcgctcg
     4381 agcaggacct cgcgagcggc gcgatccagg tcgcggtcgg cacgcacgcg ctgttccagg
     4441 aaggcgtcgg tttcgctcga ctcgggctcg tcatcgtcga cgagcagcat cgcttcggcg
     4501 tgcaccagcg cgtgaggctg cgcgagaagg gcctctccga cggccgctac cctcaccagc
     4561 tcgtcatgac cgcgacaccg atcccgcgca cgctcgcgat gaccgcgtac gccgacctcg
     4621 acgtctcggt gatcgacgag ctgccgccgg gacgcatgcc ggtgcgcacg gtcgtgctgc
     4681 cggagtcgcg gcgcgacgag gtcgtgcaac gcatcgaggc tgcgtgccgc tcgggccggc
     4741 aggcgtactg ggtgtgtccg ctgatcgacg agtccgaggc cgtgcgtcac caggccgcgg
     4801 aggaaaccgc ggcggc
//
LOCUS       DAOMFD010000155         4800 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7469_length_4800_cov_35.318230, whole genome shotgun sequence.
ACCESSION   DAOMFD010000155 DAOMFD010000000
VERSION     DAOMFD010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4800)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4800
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7469_length_4800_cov_35.318230"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PL152_08560"
     CDS             <1..212
                     /locus_tag="PL152_08560"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HQR49375.1"
                     /translation="RTPEVRNRQIVVARLENDVTVKRYRQEGTVVWLLPENQDFEPLR
                     VDLKNESLIIEGVVVGVVRQETGRA"
     gene            241..909
                     /gene="imuA"
                     /locus_tag="PL152_08565"
     CDS             241..909
                     /gene="imuA"
                     /locus_tag="PL152_08565"
                     /inference="COORDINATES: protein motif:HMM:NF033429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0051782 - negative regulation of cell
                     division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translesion DNA synthesis-associated protein
                     ImuA"
                     /protein_id="HQR49376.1"
                     /translation="MSAFDDLLRHPALWRAREQPDAGTRPATLPTGYAALDRHLPGGG
                     WPRQGLVEILTDRCGIGELSLLMPALAVLCGDDAGSGRDDASPGGWLAWVSPPYQPYA
                     PAFAARGIDLGRLLVVRAQPAEWAMEQALRSGACSAVIGWAACRDRHGLRRLQLAAEQ
                     SRSLAVLFRGLADGNDPSPAVLRIALEVGRAGLEACILKSRGGRPVTVPLGWLDPAAQ
                     AVAH"
     gene            1077..2540
                     /locus_tag="PL152_08570"
     CDS             1077..2540
                     /locus_tag="PL152_08570"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HQR49377.1"
                     /translation="MPGYVLESLRFRAAPAGAGTGQCPDATAVVDLEDGGKVVCACDA
                     AAAAAGVGPGMALNSALALLPGLHVLARDVVAERTLLEAVAARAAAGFTPRVALDPPD
                     GVLLEVRGSLRLFGGVRRLVALLREQLQSMGVEPRLALAPTPLASLWFARSGEEVALR
                     DRDGLASRLAPLPLACARWPGKSVDALATMGVRAIGDCLRLPRDGFARRFGPELLRSL
                     DRALGRAPDPRAAFASQERFVARRDLEPEIADTERLGRAIVPLLDELCRCLQARARGV
                     AAIELRLMHRDTPATRVLLRFAEPVSRVERMAGLLRERLARIELPAPVRAVRVRSGPP
                     VEVSGEDGDLFARDRRVATGVPQLVERLRARLGADAVQGLHLVPEHRPEYAWESGDIL
                     HFRVPENGECPHFRRPLWLLAQPQAIEGDEWPRHEGRLEIEEGPERIESGWWDGRDVQ
                     RDYYVARGLTGVRLWVFRDRRRRGDGAGGWFLHGVFG"
     gene            2559..>4800
                     /locus_tag="PL152_08575"
     CDS             2559..>4800
                     /locus_tag="PL152_08575"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="error-prone DNA polymerase"
                     /protein_id="HQR49378.1"
                     /translation="MTTPYAELHCLSNFSFLRGASHPEELVARAKELGYAALAITDEC
                     SVAGAVRAHVAAREHGLKLIVGSEFRLADGLRCVLLATNRNGYGQLCRLITKSRRAAE
                     KGAYRLDRTDFTHGDVSTETHSADYLNISAETSPSGRAAIDLPDCLALWLPGAQPDTD
                     EARWLAAAFRGRLWIAVELLTSGQDRRRLAVLQGLGERLGLPLVAAGDVHMHVRSRRA
                     LQDVLTATRVGTRVDRAGYALHPNGERHLRPLSRLAEIYPPGLLAATLEIAARCTFSL
                     DELRYEYPREVVPEGETATSWLRRLTEEGERRRWPRGTPATARALIEHELALIEELRY
                     EAYFLTVHDIVSHARGRGILCQGRGSAANSAVCYCLGITEVDPARMSLLFERFISRER
                     NEPPDIDVDFEHERREEVIQYIYAKYGRHRAALTATVICYRPRSAIRDVAKAMGLDPL
                     QVNALARSMQWWDGSRIDEERVREAGLDPASPVLARVIALARMLLGFPRHLSQHVGGF
                     VIARDLLEELVPVENAAMADRTVIQWDKDDLDALGLLKVDVLGLGMLAAIRRAFALVE
                     GFRGTRLTLASVPAEDPAVYEMIGHGDTMGVFQIESRAQMAMLPRLKPANYYDLVIEV
                     AIIRPGPIQGDMVHPYLRRRSGIEPVTYPSPAVEGVLRRTLGVPIFQEQVMQLAVVAA
                     GFTPGEADQLRRAMAAWKRKGGLGPFEERLIRGMRERGYAEEFAKQIFRQILGFGEYG
                     FPESHAA"
BASE COUNT          634 a         1668 c         1776 g          722 t
ORIGIN      
        1 accgcacgcc cgaggtgcgc aaccggcaga tcgtcgtcgc gcgcctcgag aacgacgtga
       61 ccgtgaagcg ctaccggcag gagggcacgg tcgtgtggct gctgcccgag aaccaggact
      121 tcgagccgct gcgcgtcgac ctcaagaacg agtcgctgat catcgagggt gtggtggtcg
      181 gcgtggtgcg gcaggagacg ggcagggcgt gagactgctc gccatccacg gcttcgttcc
      241 atgtccgcct tcgatgacct gctgcgtcac cctgcgcttt ggcgcgcccg ggagcagccc
      301 gatgcgggaa cccggcctgc cacgctgccg acgggatacg ccgcgctcga tcgccatttg
      361 ccgggcggcg gttggccgcg gcagggcctg gtggaaatcc tcacggaccg ctgcggcatc
      421 ggcgagctga gcctgctgat gccggcgctc gccgtgttgt gcggcgacga cgcaggcagc
      481 ggccgcgacg acgcatctcc cggtggctgg ctcgcgtggg tgtcgccccc ctatcagcct
      541 tatgcgcccg cgtttgcggc gcgcggcatc gacctcggcc ggctgctcgt ggtgcgcgcg
      601 cagcccgcgg agtgggccat ggagcaggcg ctgcgctcgg gggcctgcag cgccgtgatc
      661 ggctgggccg cctgccgtga tcgccacggg ctgcgtcgac tgcagctcgc cgccgagcag
      721 tcgcgcagcc tggcggtgct gttccgcggg ctggccgacg gcaacgatcc gtctcccgcg
      781 gtattgcgca tcgcgctcga agtcggccgt gcgggtctcg aggcatgcat cctgaagagc
      841 cggggcggac ggccggtgac cgtgccgctc ggctggctag accctgcggc gcaagccgtc
      901 gcgcactgac catgtcgccg tcgcggaagc gtcgggccac gcccggagtc ccgccatccg
      961 ccgcgttcct gcgggaccgc ccgaggttgc gacccgcggc tggcggtggt gatcgcggtc
     1021 caggccagcc ggctcgtgct gccgcgccgg gtcgcgagtc gtggctcgcg atccacatgc
     1081 cggggtacgt gctcgagtcg ctgcggttcc gggccgcgcc cgcaggtgcc gggaccgggc
     1141 aatgccccga tgctaccgcc gtcgtggacc tggaggacgg cggcaaggtg gtctgcgcct
     1201 gtgacgcggc cgccgccgcg gccggcgtcg ggcccggcat ggcgctcaac tcggcgctcg
     1261 cgttgctgcc gggactccac gtccttgcgc gcgacgtcgt tgccgaacgc acgttgctcg
     1321 aggccgttgc cgcgcgcgcc gccgccggtt tcacgccgcg cgtcgcactc gacccgcccg
     1381 acggcgtgct gctcgaggtg cgcggcagtc tgcggctgtt cggcggcgtg cggcggctgg
     1441 tcgccctgct gcgcgagcag ttgcagtcga tgggagtcga gccccggctc gcgctcgcgc
     1501 ctacgccgct cgcatcgctc tggttcgcgc gctcgggcga agaggttgcc ctgcgcgaca
     1561 gggacggcct tgcgagccgc ctcgccccgc tgccgctcgc ctgcgcgcgc tggccgggga
     1621 agagcgtcga tgcgctggcc acgatgggcg tgcgcgccat cggcgactgc ctccggttgc
     1681 cgcgggacgg ctttgcccgg cgattcgggc cggaactgct gcgctcgctc gaccgcgcgc
     1741 tcgggcgtgc cccggatccg cgagccgcgt tcgcgtcgca ggagcggttc gtcgcgcgcc
     1801 gcgacctcga gcccgaaatc gcggacaccg agcgcctcgg acgggcgatc gtgccgctgc
     1861 tcgacgagtt gtgcaggtgc ctgcaggcca gggcgcgcgg cgtggcggcg atcgagttgc
     1921 ggctcatgca tcgcgacacg ccggcgacgc gcgtgctgct gcgtttcgcc gagcccgtct
     1981 cgagggtgga gcgcatggcc gggttgctgc gggagcggct tgcgcgcatc gagctgccgg
     2041 caccggtgcg cgccgtccgc gtgcgaagcg gtccgccggt ggaggtgagc ggggaagacg
     2101 gggacctgtt cgcgcgtgac cggcgcgtcg ccacgggcgt gccgcaactc gtcgagcggc
     2161 tgcgcgcacg cctcggcgcg gatgccgtgc agggactgca cctcgtgccc gagcaccgtc
     2221 ccgagtatgc gtgggaaagc ggggacattc tccattttcg agtgcccgaa aatggagaat
     2281 gtccccattt tcggcggccg ctgtggctgc tcgcgcagcc gcaggccatc gagggcgacg
     2341 agtggccccg ccacgaggga cgcctcgaga tcgaggaggg gccggagcgc atcgagtcgg
     2401 gctggtggga cgggcgcgac gtgcagcgcg actactacgt ggcgcgcggc ctcaccggcg
     2461 tgcggttgtg ggtgtttcgc gatcgtcgcc gccggggcga cggcgcgggt ggctggttcc
     2521 tgcacggcgt gttcggctga actgcgcgtg ccgccgccat gacgacgccg tacgcagaac
     2581 tgcactgtct ctccaacttc agtttcctgc gcggcgcctc gcacccggag gaactcgtcg
     2641 cacgcgcgaa ggaactcggc tacgcggcgc tcgcgatcac cgacgaatgt tccgtggcgg
     2701 gggccgtgcg cgcgcatgtc gcggcacgcg agcacggatt gaaactcatc gtcggcagcg
     2761 aattccgtct cgcggacggc ctgcgctgcg tgctgctcgc caccaatcgc aacggttatg
     2821 gccagctctg ccggctgatc actaagtcga ggcgggcggc ggagaaaggc gcgtaccgac
     2881 tcgaccggac agactttacg cacggggacg tttcaaccga aacgcatagc gcagattatc
     2941 tgaacatttc agctgaaacg tcgccctccg gtcgagcggc gatcgacctg ccggactgcc
     3001 tcgcgctctg gctgccgggc gcgcaacccg acacggatga ggcccgctgg ctcgctgcgg
     3061 ccttccgcgg ccgcctgtgg atcgccgtcg agctgctgac gagcgggcag gaccggcgca
     3121 ggctcgcggt gctgcagggc ctcggcgaac gcctcggcct gccgctcgtc gccgcgggcg
     3181 acgtgcacat gcacgtgcgc agccggcgcg cgctgcagga cgtgctcacg gccacgcgcg
     3241 tcggcacccg cgtggaccgt gcgggctacg cgctgcatcc gaacggcgag cgccacctcc
     3301 gtccgctgtc gaggctcgcg gagatctacc cacccgggct gctcgccgcg acgctcgaga
     3361 tcgccgcccg ctgcacgttc tcgctcgacg aactccgcta cgaatatccg cgcgaagtcg
     3421 tgccggaagg cgagaccgcc acgagctggc tcaggcgcct cacggaggag ggcgaacggc
     3481 ggcgctggcc gcgcggcacg cccgcgaccg cccgggcgct catcgagcac gagctcgcgt
     3541 tgatcgagga actgcgctac gaggcgtact ttctcaccgt gcacgacatc gtgagccacg
     3601 cgcgcggccg cggcattctc tgccagggac gcggttcggc cgccaactcc gcggtgtgct
     3661 actgcctcgg catcaccgag gtcgacccgg cgcgcatgtc gctattgttc gagcgcttca
     3721 tctcgcgcga gcgcaacgag ccgcccgaca tcgacgtgga cttcgagcac gagcggcgcg
     3781 aggaggtcat ccagtacatc tatgcgaagt acggccggca ccgcgcggcg ctcaccgcca
     3841 cggtgatctg ctaccggccg cgcagcgcga tccgcgacgt cgcgaaggcc atggggctcg
     3901 acccgctgca ggtcaacgcg ctcgcccggt ccatgcagtg gtgggacggg tcgcgcatcg
     3961 acgaggagcg cgtgcgcgag gccggcctgg atcccgcgag cccggtgctg gcccgggtga
     4021 tcgcgctggc ccgcatgctg ctcggattcc cgcgccacct gtcgcagcac gtgggcggct
     4081 tcgtcatcgc gcgcgacctg ctcgaggaac tggtgccggt ggagaacgcc gcgatggcgg
     4141 atcgcacggt catccagtgg gacaaggacg acctcgacgc gctcggcctg ctcaaggtgg
     4201 acgtgctggg cttaggcatg ctggcggcga tccgccgcgc gttcgccctg gtcgaagggt
     4261 tccgcggcac tcggctcacg ctcgcgagcg tgccggcgga ggacccggcc gtctacgaga
     4321 tgatcggcca cggcgacacg atgggcgtgt tccagatcga gtcgcgcgcg cagatggcga
     4381 tgctgccgcg gctgaaaccg gcgaactact acgaccttgt gatagaggtg gccatcatca
     4441 ggcctgggcc gatccaggga gacatggtcc acccctatct ccggaggcgg agcgggatcg
     4501 agccggtgac ctacccgagc cccgcggtcg agggcgtgct caggcgcacg ctcggcgtgc
     4561 cgatcttcca ggagcaggtg atgcagctcg cggtcgtggc cgcggggttc acgcccggcg
     4621 aggccgacca gctgcgccgc gcgatggccg cgtggaaacg caagggcggg ctcgggccct
     4681 tcgaggaacg cctcatccgc ggcatgcgcg agcgcggcta tgcggaggag ttcgcgaagc
     4741 agatattccg gcagatcctc ggcttcggcg agtacggctt cccggagtcg catgcggcga
//
LOCUS       DAOMFD010000156         4745 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7637_length_4745_cov_35.531130, whole genome shotgun sequence.
ACCESSION   DAOMFD010000156 DAOMFD010000000
VERSION     DAOMFD010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4745)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4745
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7637_length_4745_cov_35.531130"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(37..1584)
                     /locus_tag="PL152_08580"
     CDS             complement(37..1584)
                     /locus_tag="PL152_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HQR49379.1"
                     /translation="MTVTVWLPGDSGALALGAEATARRLAGAVRATSQDVRIRRNGSR
                     GMYWLEPLVEVDCDGVRIGYGPVHARDVEGLVAAGMLRGAPHPLCLGPIDQHEWLRSQ
                     QRLTFARVGVTEPCDLADYLAHGGFRGLRRALEIGPQATVKAVTDSGLRGRGGAAFPT
                     GVKWQTVLDQPAADKFIACNADEGDSGTYADRMLMEGDPYALIEGMTIAGMATGARTG
                     YVYLRVEYPHAYRAFAAAVERARAGGYLGEDVCGSGTSFHIELRLGAGAYICGEETSM
                     LESIEGKRGEVRVRPPLPAHKGLFGAPTVVNNVVTLATVPFILAEGAAAYRDYGEAGS
                     RGTLPLQLAGNIRQAGLVERAFGLTLRELLYDYGGGAASGRPLRAVQVGGPLGAFVPE
                     ARFDTPVEYESLAGIGAMLGHGGVVAFDDTTDMLAMARYALEFCAGESCGKCAPCRIG
                     SVRGAEALDRVARGVDAERNLGIVEGICDTMTHASLCGLGGMAPAPVLSAMKHFGEDF
                     RGRRTGG"
     gene            complement(1604..2107)
                     /locus_tag="PL152_08585"
     CDS             complement(1604..2107)
                     /locus_tag="PL152_08585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit gamma"
                     /protein_id="HQR49380.1"
                     /translation="MSAGPATSTLDPAQQDVVRAVVERHRNRRGPLLEILHEVQARIG
                     YVPPAAVPLIATELNVSRADVHGVLTFYHHFRTTPPGARVLRVCRAESCQAMNGRALE
                     AHARRRLGVEFGETTGDGAVTLEAVYCLGLCAMSPAVMIDGEVHGRVSPERLDELLSA
                     DQEGRPA"
     gene            complement(2104..2970)
                     /gene="fdhD"
                     /locus_tag="PL152_08590"
     CDS             complement(2104..2970)
                     /gene="fdhD"
                     /locus_tag="PL152_08590"
                     /inference="COORDINATES: protein motif:HMM:NF001943.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HQR49381.1"
                     /translation="MDRHVRPQVLQRERLDFASTQRVPVRSVQAGEPSVRDDTVAVES
                     PLEIRVAWQDSDGAGEPVSVTMRTPGHDAELAVGFLRGEGVLRTRRDVAWVREVGPDG
                     NVVCVGLATDRTIGAARVQRNFYATSSCGVCGKASLAALENLFELSAVDAGLRVDATL
                     LTSLDAKVRDAQRIYRETGGLHSASLFDAGGGLLAIFEDVGRHNALDKLVGSMLLDDR
                     LPLAHSVLHLSGRASFELVQKAAAAGVPVVAAVGAPSSLAIDLAERSGILLVGFLRGE
                     TFNVYAHPERLA"
     gene            complement(3112..>4745)
                     /locus_tag="PL152_08595"
     CDS             complement(3112..>4745)
                     /locus_tag="PL152_08595"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF010030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HQR49382.1"
                     /translation="PMKPITDIAASLGISPDEIEPYGHYKAKVSLAAFRDRPRRGKLV
                     LVSAITPTPAGEGKTTTTIGLVQGLAKIGVKSAAALREPSLGPVFGMKGGGAGGGRAE
                     VLPMMDINMHFNGDFHAVTSAHNLLTAMVDNSLHNGNATRLDPRRVTWKRVMDMNDRA
                     LRDIVIGLGGRTEGIPRETGFDITAASEVMAVLCLADGIPDLKARLGRIVVGYTPDGT
                     PVTAREIGAVGAMSALLKEAIKPNLVQTTEGVPAFVHGGPFANIAHGTNSITATKAAL
                     AFADVVVTEAGFAFELGAEKFFDINCRYGGFAPACTVLVATIRALKMHGGKPLSEVGQ
                     PDVEAMLRGLSNLEKHVENIRKFGQPCVVAINQFAADSADEIEALRTHCESLGVQAVP
                     ARPFTEGGAGCTELAAVVRDLASRNEPRYKPLYDWNASIEEKIATVAREMYGAQAVDY
                     TARGRRDREHLEKTGFGGLPVCIAKTQQSLSDNPALLGRPKDFLVTVREIQLAAGAGF
                     VIPITGEIMRMPGLPREPMALRFDLTDDGEIVFNA"
BASE COUNT          696 a         1674 c         1649 g          726 t
ORIGIN      
        1 ggtgttccga ggtcgcggtg gttgatggcg tgcacgtcag cctcccgtcc gccggccgcg
       61 gaagtcctcg ccgaagtgct tcatggcgct cagcaccggg gcaggggcca tgccgccgag
      121 cccgcacagc gaggcgtgcg tcatggtgtc gcagatgcct tcgacaatcc cgagattgcg
      181 ctcggcgtcc acgccgcgcg ccacgcggtc gagcgcctcg gcgccgcgca ccgacccgat
      241 ccggcagggc gcgcacttgc cgcaggactc gccggcgcag aattcgagcg cgtaacgcgc
      301 catcgccagc atgtccgtcg tgtcgtcgaa ggcgaccacg ccaccgtgcc cgagcatcgc
      361 gccaatcccc gccagcgact cgtattcgac cggcgtgtcg aagcgggcct ccggcacgaa
      421 cgctcccagg ggaccaccga cctgcacggc gcgcagcggg cggccgctcg cggcgccgcc
      481 gccgtagtcg tacagcagtt cgcgcagcgt gagaccgaac gcgcgctcga cgaggccagc
      541 ctggcgaatg ttgccggcga gctgcagcgg cagcgtcccg cgcgagccgg cctcgccgta
      601 gtcccggtac gcggccgcac cttcggccag gatgaacggg acggttgcga gcgtgacgac
      661 gttgttgacc accgtcggtg caccgaagag gcccttgtgg gcgggcaggg gcggacgcac
      721 gcgcacctcg ccacgcttgc cctcgatgct ctcgagcatc gaggtttcct cgccgcagat
      781 gtacgccccg gcgccgaggc gcagctcgat gtggaaagac gtgccactcc cgcacacgtc
      841 ctcgccgagg tagccgcccg cgcgggcgcg ttcgacggcg gccgcgaagg cccggtacgc
      901 atgcggatac tcgacgcgca ggtacacata gcccgtgcgt gcgccggtcg ccatgccggc
      961 gatcgtcatg ccctcgatga gtgcgtaggg atcgccttcc atcagcatgc ggtcggcata
     1021 cgtgccggag tcgccttcgt cggcattgca ggcaatgaac ttgtcggccg ccggctggtc
     1081 caggacggtc tgccacttca cgcccgtcgg gaacgccgcg ccgccccggc cacgcaggcc
     1141 ggagtccgtc acggccttga cggtcgcctg tggcccgatc tcgagcgcgc ggcgcaggcc
     1201 gcggaaaccg ccgtgcgcga ggtagtcggc gagatcgcac ggttcggtga cgccgacgcg
     1261 cgcgaaggtg agtcgctgct gggagcggag ccactcgtgc tggtcgattg gacccaggca
     1321 cagggggtga ggcgcgccgc gcagcatgcc ggcggcgacc aggccttcga cgtcgcgcgc
     1381 gtgcacgggc ccatagccga tgcgtacgcc atcgcagtcg acctcgacga gcggttcgag
     1441 ccagtacatc ccgcgtgaac cgttccgacg aatgcgtacg tcctggcttg tcgcccgcac
     1501 agccccggcg agccggcgcg ccgtcgcctc ggcgcccagt gcaagcgcgc cggagtcgcc
     1561 gggtagccag acggtcacgg tcatcgtatg tctcctgcag gagtcacgcg ggccgtccct
     1621 cctggtccgc ggacaggagt tcgtcgaggc gttccgggct gacgcgtccg tggacttcgc
     1681 cgtcgatcat caccgcaggc gacatcgcgc acaggccgag gcagtagacg gcttcgaggg
     1741 tgacagcgcc atcgccggtc gtttcgccga actcgacgcc gagccgccgc cgtgcgtgcg
     1801 cttcgagtgc acgaccgttc atcgcctggc acgactcggc gcgacacacg cgcaggaccc
     1861 gcgctcccgg cggagtcgtc cgaaagtgat gatagaacgt gagcacgccg tgcacgtcgg
     1921 cgcgcgatac gttgagttcg gtcgcaatga gcgggaccgc ggccggcggg acgtagccga
     1981 tccgcgcctg tacctcgtgc aggatctcga gcagcggtcc gcgccggttc cggtgacgct
     2041 cgacgaccgc gcgcaccacg tcctgctgcg ccggatcgag tgtcgaggtg gcgggtccgg
     2101 cactcatgcc aggcgctccg gatgcgcgta cacattgaag gtttcgccgc gcaggaagcc
     2161 gacgagcagg atgccggagc gctccgccag gtcgatcgcc aggctcgacg gtgcacccac
     2221 ggctgcgacg accgggacgc cggccgccgc ggccttctgc acgagttcga agctcgcacg
     2281 gccgctcagg tgcagcacgc tgtgcgcgag cggcaaccgg tcgtcgagca gcatggagcc
     2341 gaccagcttg tcgagcgcat tgtgccggcc cacgtcctcg aatatggcga ggagaccgcc
     2401 gccggcgtcg aacagcgacg ccgagtgcag tccgccggtt tcgcgataaa tgcgctgtgc
     2461 atcacgcact ttcgcatcga gcgaggtgag cagtgtcgcg tcgacccgaa ggccggcgtc
     2521 gacggcggac agttcgaaca ggttctcgag ggcggcgagg gacgccttgc cgcacacgcc
     2581 acagctcgac gtggcgtaga agttgcgctg cacgcgcgct gccccgatcg tccggtcggt
     2641 cgcaaggccg acacaaacga cgttgccgtc gggcccgacc tcgcgcaccc aggccacgtc
     2701 gcgccgggtc cgcagcacgc cctcgccgcg caggaagccg accgccaatt ccgcgtcgtg
     2761 ccccggcgtg cgcatcgtca ccgagacggg ctcaccggct ccgtcgctgt cctgccacgc
     2821 cacgcggatc tcgagaggcg attcgaccgc caccgtgtcg tcgcgcacgg aaggctcgcc
     2881 ggcttgcacg gaccgaaccg gaacgcgctg cgtggatgcg aaatccagcc gctctctctg
     2941 gaggacctgc gggcgcacgt gcctgtccat ctctccccgc catagcgccc gcggaccgca
     3001 gcgagacgcc tgtggcgcga tcgtactcct cgacggaggc tgacttgtac ccctgtgccc
     3061 tgatcgcagc gcagaccctg ccgcggcctg cacgcagcca cagtcgccgg ttcaggcgtt
     3121 gaacacgatc tcgccgtcgt ccgtgagatc gaatcgcagc gccatcggtt cgcgcgggag
     3181 gcccggcatg cgcatgattt cacccgtgat cgggatgacg aatcctgcgc cggcggcgag
     3241 ctggatctcg cgcacggtca ccaggaagtc cttgggccgg ccgagcagcg ccgggttgtc
     3301 cgacagggac tgctgggtct tcgcgatgca caccggcagg ccgccgaagc cggtcttctc
     3361 gaggtgctcg cggtcgcgcc ggccgcgcgc cgtgtagtcc accgcctggg cgccgtacat
     3421 ctcccgggcc acggtcgcga tcttctcctc gatgctcgcg ttccagtcgt aaagcggctt
     3481 gtaccggggt tcattgcggg acgccaggtc gcggaccacc gcagcgagct ccgtgcagcc
     3541 ggcgccgccc tcggtgaagg gacgggccgg cacggcctgt acgccgagcg actcgcagtg
     3601 tgtcctgaga gcctcgatct cgtcggccga gtcggcggcg aactggttga tcgcgaccac
     3661 gcagggctgg ccgaacttgc ggatgttctc gacgtgcttt tccaggttcg agaggccgcg
     3721 cagcatcgcc tcgacgtcgg gctgcccgac ttcggacagg ggcttgcccc cgtgcatctt
     3781 gagcgcgcgg atcgtggcga ccagcacggt gcacgccggc gcgaagccgc cgtagcgaca
     3841 attgatgtcg aagaacttct cggcgccgag ctcgaaggcg aacccggcct ccgtcaccac
     3901 gacgtcggcg aaagccaacg ccgctttcgt cgccgtgatg ctgttggtcc cgtgcgcgat
     3961 gttggcgaac ggcccgccgt ggacgaaagc gggcacgcct tcggtggtct gcacgaggtt
     4021 cggcttgatc gcctccttga gcagggcgga catggcgccg accgccccga tctcacgcgc
     4081 ggtgaccggc gtcccgtccg gcgtgtaacc cacgacgatg cgaccgaggc gcgccttgag
     4141 gtccgggatg ccgtccgcca ggcacagcac cgccatgacc tcgctcgcgg ccgtgatgtc
     4201 gaacccggtc tcgcgcggaa tgccctcggt gcgaccgccg agcccgatga cgatgtcgcg
     4261 cagcgcgcgg tcgttcatgt ccatgacccg cttccaggtc acgcggcgcg ggtcgagtcg
     4321 cgtcgcgttg ccgttgtgca ggctgttgtc gaccatcgcg gtgagcaggt tgtgcgcact
     4381 ggtcacggcg tggaagtcgc cgttgaagtg catgttgatg tccatcatcg gcaggacttc
     4441 cgcgcggccg ccgccggcgc cgccgccctt catgccgaag accgggccca ggctcggctc
     4501 gcgcagcgcc gccgcgctct tcacgccgat cttcgcgagg ccctgcacca ggccgatcgt
     4561 ggtggtcgtc ttgccttcgc ccgccggtgt cggcgtgatc gcggacacca gcacgagctt
     4621 gccgcgacgc gggcggtccc ggaatgcggc gagcgatacc ttggccttgt agtggccgta
     4681 aggctcgatc tcgtcggggc tgatgccgag ggaggcggcg atgtcggtga tgggcttcat
     4741 cggtc
//
LOCUS       DAOMFD010000157         4665 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7849_length_4665_cov_30.601085, whole genome shotgun sequence.
ACCESSION   DAOMFD010000157 DAOMFD010000000
VERSION     DAOMFD010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4665)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4665
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7849_length_4665_cov_30.601085"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1265)
                     /locus_tag="PL152_08600"
     CDS             complement(<1..>1265)
                     /locus_tag="PL152_08600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49383.1"
                     /translation="TLTIPSPAELQAQGAVIGRIIVIPDNIFDTSIPGESGWLYRTAN
                     KLHIKTRESVIRGQLLFKKGDPYDARLVQETERILRGNSFLYDARITPVAYDGHTVDL
                     EVRTRDVWTLNPGLNFGRAGGKNELNVHVQEQNLFGTGRKVEVAWDSNVDRNALTFSF
                     VDPHFLQAFTRLAVAYSDATDGSTKFLKLERPFYALDTRRAAGTTLADSEFTDYRWEL
                     GKKVGQFRDHDQYYDLGGGWSPGLVAGWANRYTFGATYHEQQFAPDDDKPLGGPLPPD
                     QKFVYPWIGYERVQDAFQERVNQDQIMRTEDVLVGFRGWARLGYASAALGSESSALIL
                     DSGVQDGADLTPRQSLFGSAWASGRYERGEIRNGILGAESHYYLQTSERSKFYARLSG
                     TVTRNLDENLQLTLGGDTGLRGYPYAYQAG"
     assembly_gap    1266..1365
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1729..2703)
                     /locus_tag="PL152_08605"
     CDS             complement(1729..2703)
                     /locus_tag="PL152_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HQR49384.1"
                     /translation="MSYAIRIHAFGGPEVMQWESYDPGEPARGQALIRQTAIGLNFID
                     VYERTGLYPNVLPLVLGREAAGAVEAVGPGVKGLAVGDRVAYVSGQPGAYAQHRVMPA
                     DRLVKIPEGVSDRLAAAAMLKGLTAQFLLRRTHRVKKGDTVVIHAATGGVGTIATQWA
                     RHLGATVVGVVGSEAKVAAALAHGCHHALVTGRDDLVARVREISGGQGADVVYDSVGK
                     DTFFPSLDCLRPLGLMVSYGNASGPVPAIQPLELSKRGSLFLTRPTLFHYIARRADLE
                     RSARELFDVIGKGVVRIEIGQTHALQDVARAHRDLEGRRTVGSTVLIP"
     gene            complement(2815..3174)
                     /locus_tag="PL152_08610"
     CDS             complement(2815..3174)
                     /locus_tag="PL152_08610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49385.1"
                     /translation="MPDPLDTALAKLRLGRQSRHVFLCVGGKCAPREQALQSWEHLKR
                     RLAELGLPDADGGVLRTKADCLRVCTAGPIALVYPDGTWYRDCTPANLDRIIDEHLLR
                     GRPVADLVIAEAPLGRG"
     gene            complement(3179..3517)
                     /locus_tag="PL152_08615"
     CDS             complement(3179..3517)
                     /locus_tag="PL152_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HQR49386.1"
                     /translation="MKLISAIIKPFKLDDVRAALSELGVSGMTVTEVKGFGRQRGHTE
                     LYRGAEYVVDFVPKTRIEVAVKDGLVDQVVEAIVGAARTGKVGDGKIFITDVERAIRI
                     RTGEADDQAL"
     gene            3825..>4665
                     /locus_tag="PL152_08620"
     CDS             3825..>4665
                     /locus_tag="PL152_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR49387.1"
                     /translation="MDLKERTLFISGASRGIGLAIALRAARDGANVVVAAKTAEPHRK
                     LPGTIHTAAREIEAAGGHALPVLLDVRDEAQVAAVVEQAAGHFGGIDVLVNNASAISL
                     TGTLQTPARIFDRMMNVNTRGTFLCSQACIPHLRKSANPHILNLSPPLNLDPKWFGGH
                     VAYTIAKYGMSLCVLGMARELARDGIAVNALWPRTAIATAAIEFAVGGAGMLRHCRKP
                     EILADAAHWILTRPSRELTGQFLVDDEVLGRAGVTDFARYAVDPAAPLALDLFLDDKG
                     PYIK"
BASE COUNT          681 a         1613 c         1557 g          714 t
ORIGIN      
        1 ccggcctggt aggcgtacgg atagccgcgc agcccggtgt cgccgccgag cgtcagctgc
       61 aggttctcgt cgaggttccg ggtcaccgtg cccgaaagac gcgcgtagaa cttgctgcgc
      121 tccgacgtct ggaggtagta gtggctctcg gctcccagga tcccgttgcg gatctcgccg
      181 cgctcatagc ggcccgaggc ccacgccgag ccgaacagcg actgtctcgg cgtgaggtcg
      241 gcaccgtcct ggacgccgga gtccaggatc agggcactcg actccgaacc gagcgcggcg
      301 ctggcatagc cgagccgcgc ccagccccgg aagccgacca gcacgtcctc ggtgcgcatg
      361 atctggtcct ggttgacgcg ctcctggaac gcgtcctgca cgcgctcgta cccgatccac
      421 ggatagacga atttctggtc gggcggcaac gggccgccga gcggcttgtc gtcgtccggc
      481 gcgaactgct gctcatggta ggtcgcgcca aacgtgtagc gattcgccca ccctgcgacg
      541 aggcccggcg accagccgcc gccgagatcg tagtactggt cgtggtcgcg gaactggccg
      601 accttcttgc cgagttccca gcgatagtcc gtgaattcgc tgtcggccag cgtcgtgccg
      661 gcggcgcggc gcgtgtcgag ggcgtagaac gggcgctcga gtttcaggaa cttcgtgctg
      721 ccgtcggtcg cgtcgctgta ggcaactgca agccgcgtga acgcctgcag gaaatgcgga
      781 tcgacgaacg agaaggtcag cgcattgcga tccacgttgc tgtcccaggc gacctccacc
      841 ttgcgcccgg tcccgaacag gttctgttcc tggacgtgca cgttcagctc gttcttgccc
      901 ccggcgcggc cgaagttcag gcccgggttg agcgtccaga cgtcgcgggt gcgcacctcc
      961 aggtccaccg tgtgcccgtc ataggcgacc ggcgtgatgc gcgcgtcgta gaggaagctg
     1021 ttgccgcgca ggatgcgctc ggtctcctgg accagccgcg cgtcgtacgg gtcgcccttc
     1081 ttgaacagca gctggccgcg gatcaccgac tcgcgggtct tgatgtgcag cttgttcgcg
     1141 gtgcgataga gccagccgct ctcgccggga atcgaggtgt cgaagatgtt gtccgggatc
     1201 acgatgatgc ggccgatcac ggcgccctgc gcctggagtt cggccggcga cggaatggtc
     1261 agggtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnggccg atcacggcgc
     1381 cctgcgcctg gagttcggcc ggcgacggaa tggtcagggt cgttccagcg gtcgcgcttg
     1441 cgggagcggc cgcagccgcc gtcggactgg caggcgccgc agttggcgct ggtgcagatc
     1501 cggcgggcgt cggcgattcc tccagcggtg cgggttcggt cagtgcgggc gactcgtgaa
     1561 gcgtcgaggg ttcctgcagc ggcgctggca cgtcgacctg cgtcgcctcc tccggcgtcg
     1621 ccggagtttc gtccgcatgg acgccgcccg ccgccatgaa gacgatcgcc aacgccacgc
     1681 agcggccggt gccacgcgtt gtcgatcgca atcgcactgc aactcgcgct acgggatgag
     1741 gacagtcgag ccgacggtgc gtcgaccctc gaggtcgcga tgtgcgcgcg ccacgtcctg
     1801 cagcgcgtgc gtctgtccga tctcgatgcg caccacgcct ttgccgatca cgtcgaacaa
     1861 ctcgcgtgcc gaccgctcta ggtccgcacg ccgtgcgatg tagtggaaca gcgtggggcg
     1921 cgtcaggaac agcgagccac gtttcgacag ttcgaggggc tggatggccg gcaccggccc
     1981 cgacgcgttg ccgtagctca ccatcagccc gagcgggcgc aggcagtcta gcgacgggaa
     2041 gaacgtgtcc ttgccgaccg agtcgtacac cacgtcggcg ccctggccgc cggaaatctc
     2101 gcgcacgcgg gccacgaggt cgtcgcggcc cgtcacgagc gcgtgatggc agccgtgtgc
     2161 gagcgcggcc gcgactttcg cttcgctgcc gaccacgccg accacggtcg cgccgaggtg
     2221 gcgcgcccac tgcgtcgcaa tcgtcccgac gccgccggtg gccgcgtgga tcaccacggt
     2281 gtcgcctttc ttcacgcggt gcgtgcggcg gagcaggaac tgcgccgtga gccccttcag
     2341 catggcggcc gcagcgagcc ggtcgctcac gccctcgggg atctttacca gccggtcggc
     2401 gggcatgacg cgatgctgcg cataggcgcc cggttgcccc gagacgtacg cgacgcgatc
     2461 accgaccgcc agccccttca cacctggacc cactgcttcc accgctcccg ccgcctcgcg
     2521 acccaggacc aggggcagca cgttcggata caggcccgtg cgctcgtaga cgtcgatgaa
     2581 gttcaggccg attgcggtct ggcggatcag cgcctggcca cgcgccggct cgcccgggtc
     2641 gtacgactcc cactgcatga cctcggggcc gccgaaggcg tggatgcgga tggcgtatga
     2701 catggcaggg agggaccagt gaacccaggt ccatggatag tgaacaagcg ggtgcccggc
     2761 ggcaaccgcg cctatccact atgccgcggc cgctatccgc aaggacgcgg ccgctcagcc
     2821 ccgtccgagc ggcgcctcgg caatcacgag gtccgccacc ggccggccgc gcagcaagtg
     2881 ctcgtcgatg atccggtcga gattcgcggg cgtgcagtcc cggtaccacg tgccgtccgg
     2941 gtacacgagg gcgatgggcc cggccgtgca gacccgcagg cagtcggcct tggtgcgcag
     3001 cacgccgccg tccgcatccg gcaggccaag ttccgccagg cgacgcttca ggtgctccca
     3061 gctctgcagg gcctgctcgc ggggcgcgca ctttcctccc acgcacagga agacgtgccg
     3121 cgactggcgg ccgagccgga gcttcgcgag cgcggtgtcg agcggatcgg gcaagccgtc
     3181 agagggcctg gtcgtccgcc tcgccggtcc ggatgcggat cgcgcgctcg acgtcggtga
     3241 tgaagatctt gccgtcgccg accttgccgg tgcgcgcggc acccacgatg gcctcgacga
     3301 cctggtccac gagcccgtcc ttgacggcga cctcgatgcg ggtcttgggc acgaagtcga
     3361 cgacgtactc ggcgccgcgg tacagctcgg tgtggccgcg ttgccggccg aagcccttca
     3421 cctcggtgac cgtcatcccg gacacgccga gctccgacag cgccgcacgc acgtcgtcca
     3481 gcttgaaggg tttgatgatt gcgctgatca gcttcatagg gctctggctc ctggaatcct
     3541 ggtccttctc cggcggagat ctctcccgcc actatgcaga aaccgtgccg ggtcgcggtc
     3601 cggccatttc cggccctccc ggcggccggt ccgcaccagc atgtggcagc ccccggaccg
     3661 ccctgcccga ccgtggtgcg cggaccccgc aagcatagcc atgcgcgggc ggcggcgcac
     3721 cctgttccac gaacccgggc aacgggaacc gccgcgggct gcgccggact ccgcggacgg
     3781 gcatcatgcc gcccttccga catcgacgag gacggccgac cgatatggac ctcaaggaac
     3841 ggaccctgtt cataagcggt gcgagccgcg gcatcgggct cgccatcgct ctgcgtgccg
     3901 cgcgcgatgg cgcaaacgtc gtggtcgcgg ccaagaccgc ggagccgcac cgcaagctgc
     3961 cgggcacgat tcacacggcg gcccgcgaga tcgaagccgc cggcggtcat gccctgcccg
     4021 tcttgctgga cgtccgcgac gaggcgcagg tcgcggccgt cgtcgagcag gctgccggtc
     4081 acttcggcgg catcgacgtg ctcgtgaaca acgccagtgc catcagcctc accggcaccc
     4141 tgcagacgcc tgcccgcatc ttcgaccgga tgatgaacgt gaacacccgc ggcacgttcc
     4201 tctgcagcca ggcctgcatc ccgcacctgc gcaagtccgc gaacccgcac atcctcaacc
     4261 tgtcgccgcc gctcaacctg gacccgaagt ggttcggcgg gcacgtcgcg tacacgatcg
     4321 ccaagtacgg catgagcctg tgcgtgctcg gcatggcccg cgaactcgcg cgtgacggga
     4381 tcgcggtgaa tgcgctgtgg ccgcgcaccg caattgccac ggcggccatc gagttcgccg
     4441 tcggcggcgc cgggatgctg cgccactgcc gcaagccgga gatcctggcc gacgccgcgc
     4501 actggatcct cactcgcccg agccgggaac tcaccgggca attcctcgtg gacgacgagg
     4561 tgctcggccg cgccggcgtg acggatttcg cccgctatgc cgtcgacccg gcggcgccgc
     4621 tcgcgctcga cctgttcctc gacgacaagg ggccgtatat aaagg
//
LOCUS       DAOMFD010000158         4632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_7938_length_4632_cov_32.857112, whole genome shotgun sequence.
ACCESSION   DAOMFD010000158 DAOMFD010000000
VERSION     DAOMFD010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4632
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7938_length_4632_cov_32.857112"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1243
                     /gene="maeA"
                     /locus_tag="PL152_08625"
     CDS             <1..1243
                     /gene="maeA"
                     /locus_tag="PL152_08625"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: protein motif:HMM:NF010052.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="oxaloacetate-decarboxylating malate
                     dehydrogenase"
                     /protein_id="HQR49388.1"
                     /translation="NESILGIGDQGAGGMAICVGKLALYTVAAGIPPAATLPVSLDVG
                     TDNPGLLGDSMYVGWREPRLRGAEYDDFVEEFVDAVAMVFPKALLQWEDLRKDIALRV
                     ADRYRTRLPSFNDDIQGTGAVALAGLLASGRVTGTGLSEQRVVVHGAGAAGLGIVRQI
                     RAAMRLEGLSETQCVERVAALDSRGLLVADREQRDAYKRDLAWPPSLVQALGIGAERG
                     LDAVVRAFRPTALVGTSGQKGCFTESIVRAMAQGCERPVVLPFSNPSDLSEATPADLL
                     RWTEGRALVATGSPFAPVDVGGRAVRVGQGNNVLVFPGLGLGVLLGGLRTVTDEMLTA
                     AAFTLAGKVTDAELASGMLFPAVTRLREVSAAVAAAVIATARGKDVREPPSPALVDAV
                     AAKMWVPRYEEYVAVASDAAE"
     gene            1249..1884
                     /locus_tag="PL152_08630"
     CDS             1249..1884
                     /locus_tag="PL152_08630"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HQR49389.1"
                     /translation="MGPVALCVVAVAALLAAERRRSRTGMVIAKPVASAAFLWAALQW
                     GALETHYGTLVLAGLALCMLGDVLLIPTDRPRVFRAGVFAFLLGHVAYASAFLTRPLS
                     PPGLLAGGVLLVLVLGMVLRWLGRSLPPDMVVPVRAYMIVIGVMATLACGVTAAGGPW
                     QVVVGALAFTASDVSVARDRFVRQAFVNRAWGLPLYYVSQLLIAATPALVR"
     gene            complement(1952..2266)
                     /locus_tag="PL152_08635"
     CDS             complement(1952..2266)
                     /locus_tag="PL152_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49390.1"
                     /translation="MSIRKIVTAVTAAVVLAPAAFAATTPAASSNPAAAPAPATTPAA
                     QCDSLAKEYQTAAAANSSNPNAAKAKKAAEEGAKLCKEGKPADGVKKYEHALKELGVK
                     AK"
     gene            2488..3204
                     /locus_tag="PL152_08640"
     CDS             2488..3204
                     /locus_tag="PL152_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR49391.1"
                     /translation="MDSSPHILIVDDDREIRDLLSRFLERNGLRVSVARDEREMQKVL
                     QDAAINLVVLDAMLPGKNGFTICRELRAAGNLPIIMLTAAGDETDRIVGLEMGADDYV
                     AKPFNPRELLARMRAVLRRAAEPGRAIEAPRGVLGFAGWTLDVARRQLLSPEGALVTL
                     STGEFDLLLAFAEHPQRVLSRDQLLDIARGRGPAPFDRSIDVQVSRLRRKIEVDPRAP
                     EIIATVRGGGYVFTPAVERK"
     gene            3201..4613
                     /locus_tag="PL152_08645"
     CDS             3201..4613
                     /locus_tag="PL152_08645"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR49392.1"
                     /translation="MKRLLPDTLAGWVLVVLIGGLALSQMATLAANYRTRTSTATVLE
                     HFRLAERIADIVRLVGSSPPEQRPRMLSSLAGRTLRASWGPAPAIADVPPGNWHAQLL
                     DELVQSALWDVPWRELRVSLAQAPGDASGRSSKLPDRDSAATKDVARSVQEIIAEHNR
                     ASVLRIALQLDDQTWLNFEAPFVEERNVAPAWSVPLLVVAGLVILFASAWAVRRLTAP
                     LTTLADAAEQLGRDVNAAPLPESGPSELRQAAHAFNLMQERIQRFVRDRTLMVAAMSH
                     DLRTPITRLRLRAEFIEDEEQRRRTLGDLADMEAMIDSTLAFAREESSTEADASADLV
                     SLLGSVCEDRPGVTLSFGPGIGSRLPYVCRPIALARCVANVVDNAVKYGGRATVQLFA
                     DAASVTIRVDDEGPGIAATDRERAFAPFTRLEPSRNQDTGGTGLGLTIARTIARAHGG
                     EVTLGDRPGGGLRVDITLPR"
BASE COUNT          585 a         1580 c         1724 g          743 t
ORIGIN      
        1 caacgagtcc atcctcggca tcggcgacca gggcgccggc ggcatggcga tctgcgtcgg
       61 caagctcgcg ttgtacaccg tcgcggccgg gatcccgcca gccgcgacgc tgcccgtcag
      121 cctggacgtc ggaaccgaca acccgggcct gcttggcgac agcatgtacg tgggctggcg
      181 cgagccgcga ctgcgcggcg ccgaatacga cgacttcgtc gaggagttcg tcgacgccgt
      241 cgcgatggtg tttcccaagg cgctgttgca atgggaggac ctgcgcaagg acatcgcgct
      301 gcgcgtggcg gaccgctacc gcacgcgtct gccttccttc aacgacgaca tccagggtac
      361 cggcgcggtc gcactcgccg ggctgctggc gtcgggtcgc gtcaccggta cgggactgtc
      421 ggaacagcgc gtggtcgtgc acggggcggg cgcggcggga ctcggcatcg tgcggcagat
      481 ccgcgccgcg atgcggctcg agggcttgag cgagacgcag tgcgtggaac gcgtcgccgc
      541 tctcgacagc cgcgggctgc tcgtcgccga ccgcgagcag cgcgacgcgt acaagcgcga
      601 cctcgcctgg ccgccgtcgc tcgtgcaggc acttgggatc ggcgcggagc gcggcctcga
      661 cgcggtggtg cgggcgttca ggccgacggc gctcgtgggc acgtccggcc agaaggggtg
      721 cttcacggag tccatcgtgc gggcaatggc tcagggctgc gagcggcccg tggtgctgcc
      781 gttctccaac ccctcggacc tgagcgaggc gactccggcg gacctgttgc gctggacgga
      841 aggtcgtgcg ctcgtcgcga ccggcagccc gttcgccccc gtggacgtcg gcggacgggc
      901 ggtgcgcgtg gggcagggca acaacgtgct ggtattcccc gggctcggcc tcggcgtcct
      961 gctcggaggg ttgcgtacgg tgacggacga gatgctcacc gccgcggcct tcacgctggc
     1021 cggcaaggtc acggacgcgg aactcgcgag tggcatgctg ttcccggccg tgacgcgact
     1081 gcgggaggtc tcggcggcgg tggcggctgc cgtgatcgcg acggcgcgcg gcaaggacgt
     1141 gcgggagccg ccgtcgcccg ccctcgtcga cgcggttgct gcgaagatgt gggtgcctcg
     1201 ctacgaggaa tacgtcgccg tggcgagcga cgccgccgaa tgatggcgat gggaccggtg
     1261 gcgctgtgcg tcgtggccgt cgccgccttg ctggcggccg agcgtcgtcg atcgcggact
     1321 ggaatggtga tcgcaaagcc ggttgcgtcg gcggcctttc tctgggcggc actgcagtgg
     1381 ggcgcgctcg agacacatta cggcaccctg gtgctggcgg ggcttgcgct gtgcatgctc
     1441 ggggacgtgc tgctgatccc gaccgaccgg ccccgggtct ttcgtgcggg ggtgttcgcg
     1501 ttcctgctcg ggcacgtcgc ctatgcgtcg gcattcctca cgcggccact gtcgccgccg
     1561 gggctcctgg cgggcggggt gctgctggtg ctcgtgctcg gcatggtgct ccgctggctc
     1621 ggtcgttcgc tcccgccgga catggtggtc ccggtgcggg cgtacatgat cgtgatcggg
     1681 gtgatggcga cgctcgcttg cggggtcacc gcggccggtg gcccatggca ggtcgtggtc
     1741 ggcgcactgg cgttcaccgc gtcggatgtc tcggtggcgc gcgaccggtt cgtgcggcag
     1801 gcgttcgtga accgggcgtg gggactgccg ctctactacg tgtcgcaatt gctgatcgcc
     1861 gcgacgccgg ccctcgtccg ctgagccagc cccgaaatgc aacggggccg gcccttgcgg
     1921 accggccccg gtcgtcacgc gatgcgcgtg attacttggc cttgacgccc agttccttga
     1981 gcgcgtgctc gtacttcttc actccgtcgg ccggcttgcc ttccttgcac agcttggcgc
     2041 cctcttcggc ggccttcttc gccttggcgg cgttcgggtt gctcgagttg gcggccgccg
     2101 cggtctggta ctccttggcc agcgagtcgc actgcgcggc cggggtcgtc gcgggcgccg
     2161 gggcggcggc cgggttgctg gacgcggcgg gcgtcgtggc agcgaacgcg gccggggcga
     2221 gcacgacggc ggcggtgacg gcggtgacga tcttgcggat gctcatggtc tcgatctcct
     2281 gtctcagtgg gtggttgact gtcccatccg gggtattccg gatgggtgtc atgagacgcc
     2341 cggcgtgtaa gcggagtgtt tcgggactgg ccgccgcccg taacggactg ttgcgacacc
     2401 gctggctgta acattccgtt acaaagtcgc ggcgcgagtg gctggccccc ggctatggtg
     2461 gggcccgagc gcccgccggt agtgaacatg gattcgtctc ctcacatcct catcgtcgac
     2521 gacgatcgcg agatccgcga cctcctgtcg cgtttcctcg agcgcaacgg gctgcgagtc
     2581 tcggtcgcgc gcgacgagcg cgagatgcag aaggtgctgc aggacgcggc catcaacctc
     2641 gtcgtgctgg acgccatgct gccgggcaag aacggcttca cgatctgccg cgaactgcgc
     2701 gccgcgggca acctgccgat catcatgctc acggccgccg gggacgagac cgaccgcatc
     2761 gtcggcctcg agatgggcgc agacgactac gttgccaagc cgttcaatcc gcgggagctg
     2821 ctcgcccgca tgcgggcggt cctgcggcgc gccgcggagc ccgggcgtgc gatcgaggcg
     2881 ccacgcggcg tgctcggctt cgcgggctgg acgctggacg tcgcgcgtcg gcagctgctc
     2941 tcgccggaag gcgccctggt gacgctgtcc accggcgagt tcgacctgct gctcgcgttc
     3001 gccgagcatc cccagcgggt gctgagccgc gaccagttgc tcgacatcgc gcgcggccgc
     3061 ggtccggcgc ccttcgatcg cagcatcgac gtgcaggtga gccggctgcg ccgcaagatc
     3121 gaggtcgacc cgcgcgcgcc cgaaatcatc gcgaccgtgc gcggcggcgg ctacgtcttc
     3181 acgccggccg tggagcgcaa gtgaagcggc tgctcccgga taccttggcc gggtgggtgc
     3241 tcgtcgtgct gatcggcgga ctcgcgctgt cgcagatggc gacgctcgcc gccaactacc
     3301 gcacgcgcac cagcacggcg accgtgctcg agcatttccg gctcgcggaa cgcatcgcgg
     3361 acatcgtgcg cctcgtgggc agctcgccgc cggagcagcg cccgcggatg ctctcgagcc
     3421 tcgccggccg gacgctccgc gcgagctggg gcccggcccc ggcgatcgcc gacgtgccgc
     3481 ccggtaactg gcacgcgcag ctgctcgacg aactggtgca gtcggcgctg tgggacgtgc
     3541 cgtggcgcga gctgcgcgtg tcgctggcgc aggccccggg cgacgcgtcg ggccgttcgt
     3601 cgaagctgcc cgaccgggac agcgcggcca ccaaggacgt ggcgcgcagc gtccaggaga
     3661 tcattgccga gcacaaccgc gcctcggtgc tgcgaatcgc gctgcagctc gacgaccaga
     3721 cgtggctcaa tttcgaggcg cccttcgtcg aggagcgcaa cgtcgcgccg gcgtggtcgg
     3781 tgccgctgct ggtcgtcgcc ggcctcgtga tcctcttcgc gtcggcgtgg gcggtgcgcc
     3841 gcctgacggc gccgctcacg acactggccg acgcggcgga acagctcggc cgcgacgtga
     3901 atgccgcacc gctgcccgag agcgggccgt cggagctgcg gcaggccgcg cacgccttca
     3961 acctcatgca ggagcgcatc cagcgcttcg tgcgggaccg caccctgatg gtcgccgcca
     4021 tgtcgcacga cctgcgcacg ccgatcacgc ggctcaggct gcgcgcggag ttcatcgagg
     4081 acgaggagca gcggcgccgg acgctcggcg acctcgcgga catggaagcg atgatcgact
     4141 cgacgctggc cttcgcgcgg gaggaatcca gcaccgaggc ggatgcgagt gccgatctcg
     4201 tgtcgctgct gggcagcgtt tgcgaggacc ggccgggtgt cacgctctcg ttcgggcccg
     4261 gcatcgggtc gcgtctgccc tacgtctgtc gacccatcgc gctggcacgt tgcgtggcca
     4321 acgtcgtgga caatgccgtc aagtacggcg gccgcgccac ggtgcagctg ttcgccgatg
     4381 ccgcgagcgt caccatccgg gtggacgacg agggaccggg catcgccgcg acggaccgtg
     4441 agcgcgcatt tgccccgttc acgcggctcg agccgtcgcg caaccaggac acgggcggca
     4501 cgggcctcgg gctcacgatc gcccgcacca tcgcgcgtgc gcacggcggc gaggtcaccc
     4561 tcggcgaccg ccccggcggc gggctgcggg tcgacatcac gctcccgcgc tgacgggaaa
     4621 atggggacat tc
//
LOCUS       DAOMFD010000159         4575 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8120_length_4575_cov_26.729867, whole genome shotgun sequence.
ACCESSION   DAOMFD010000159 DAOMFD010000000
VERSION     DAOMFD010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8120_length_4575_cov_26.729867"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            207..2861
                     /locus_tag="PL152_08650"
     CDS             207..2861
                     /locus_tag="PL152_08650"
                     /inference="COORDINATES: protein motif:HMM:NF023649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HQR49393.1"
                     /translation="MSVQRIYATAIGVIAFGLLAACGQKAPKEPEQAGTPAAAGQAAS
                     AGAAEDLKPNPDRNAYFGETHIHTSWSVDAWLMGNRVTGPDEAYKYAQGQTIKHPLGY
                     DIKIETPMDFMGVTDHSEYVGVTKEANTPGSALSKLPEAQPLILKDPNDQAEIQKTFT
                     YLVNMLSQPPVKAFMSPEVAGGIWKKNVDIADANNHPGRFTAFCSYEFTSQNNNINLH
                     RNVFFRDCAKVPLMPFSALDSWHPEDLWKWMDTQRKAGNELLAISHNANLSSGWMYPT
                     DVDSYGRPIDAAWAAARDRNERLIEIKQIKGASETHPLLSPNDEFASYELTSGLLGLP
                     ATAGRVDKIQGSYGRQALKDGLTLQDVSGFNPYKFGFGAASDSHNTGSPYRQDNFFGG
                     HAQLDGDVQRRMAGVLAFGTLDVRLENPAGLTGVWAEENTRASIWDAMYRKETFGVSG
                     PHIKVRFFGGWNYAKNAFDAKDWVHQSYANGVPMGGDLPPMPAGGKGTAPTFVVWAVK
                     DPTSGSLDRIQVIKGWSQNGQSFEKIYDVVWAGDRKPDPWTGRVPAIKSTVDLAKATY
                     TNDEGSTELKTVWTDPDFDASQHAFYYARVLEIPTPRWSLIQAVKAGIPPPDVVPLTG
                     QERAWSSPIWYTPSAEARKAAPAGMTVAALKAKGATQLNDAQLKSFIVGKAYWIRNNV
                     TGDQFSVSYTADGGSIVWHVGKYANTPSLTGELMRNGYFGQTTPYKIANGRVVTNLQE
                     APFSIAIYKLGDSYYAARSNEFGYANYEVIPDPQFAVNPLMSMFNQLSIELGLTAQQQ
                     REIKPILKSELTQLEALKKDTSMSAEQKLEKLKAASAAIDAQITPLLNAEQQPKFQAV
                     RDEMRRHLLETMAGKAGKALESKIESHM"
     assembly_gap    2897..2996
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2997..3328)
                     /locus_tag="PL152_08655"
     CDS             complement(<2997..3328)
                     /locus_tag="PL152_08655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49394.1"
                     /translation="MPNGQHKVTLTVDSSNRFQFTGGTDRHGKCENRVRDGAAPVKIT
                     LAAPEGYTITSVDLVGKGSAQMKADVIGDGKSAKIDNKCEDKADVEYTVNVTTPGGIN
                     IPCHPRIV"
     gene            complement(3501..>4575)
                     /locus_tag="PL152_08660"
     CDS             complement(3501..>4575)
                     /locus_tag="PL152_08660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR49395.1"
                     /translation="LGGAFESMQRYMDYSRELARRAPDNDAYLLELSYAYSNLGSVAL
                     AQGRPEVALEEFRRSVALAGLLATKSPDDYDLAFNLADTRSWIGTTLLELGRLADARD
                     EFAMAVQAMRPFHVKAQDQRASYNYCRLLILQADADINRGNVAEAKRALGESLAVYRT
                     LLENDPTNTTWRYNALTAETVLLGLIPPGEWTAQERASLQRIESTLGGASSADASDKD
                     YIRLEFRVRNLRDIVLLHQGEPQASLRAARQTHRDWQAARRGKTMIPEFTLIEARVEE
                     TLGSALAAAGDLTGARAVWQAEVERLDDKPTTNLSLAAVRRLLAIDLGDAARADAIAA
                     QLEAAGYRDPRSDPAYTRSGAYR"
BASE COUNT          814 a         1480 c         1479 g          702 t
ORIGIN      
        1 atttccgtgc acgccgcgct ggtgcaggcc gcgcagcacg cggattccgg ccagggtacg
       61 tccggggccg tccacccgca ctctccgttg acaaggggga cgtagaggca aaggattgca
      121 cggaaaagca gcagccgatg ctcgctcccc caccgccgac agtgctgggt cttcgaactt
      181 tttgcatgag ccacgaggaa tgtgccatga gcgtccaacg tatctacgcg acggcgatcg
      241 gagtcatcgc attcgggttg ctggctgcct gcggccagaa ggcgccgaaa gagcctgaac
      301 aggcgggcac tccggccgcc gcgggccagg cggcctccgc gggtgcggcc gaagacctga
      361 agcccaaccc cgaccgcaac gcctatttcg gcgagacgca catccacacc agctggtcgg
      421 tggacgcgtg gctcatgggc aaccgcgtca ccggtccgga cgaggcctac aagtacgcgc
      481 aggggcagac catcaagcac ccactcgggt acgacatcaa gatcgagacg ccgatggact
      541 tcatgggtgt cacggaccac tccgagtacg tcggcgtcac gaaggaagcg aacacgccgg
      601 gttcggcgct ctccaagctg ccggaggccc agccgctgat cctgaaggac ccgaacgacc
      661 aggccgagat ccagaagacc ttcacgtacc tcgtgaacat gctgagccag ccgccggtca
      721 aggccttcat gtcgcccgag gtcgcgggcg gcatctggaa gaagaacgtc gacatcgccg
      781 acgcgaacaa ccatcccggc aggttcacgg cgttctgttc gtacgagttc acgtcccaga
      841 acaacaacat caacctgcat cgcaacgtct tcttccggga ctgcgccaag gtgccgctga
      901 tgcccttcag cgcgctcgac tcctggcacc cggaagacct gtggaagtgg atggacaccc
      961 agcgcaaggc gggcaatgaa ctgctcgcaa tctcgcacaa cgccaacctg agcagcggat
     1021 ggatgtatcc gaccgacgtc gacagctacg ggcgcccgat cgacgccgcg tgggcggctg
     1081 cccgcgaccg caatgagcga ctgatcgaga tcaagcagat caagggggca tcggagacgc
     1141 acccgctgct gtcgcccaac gacgagttcg ccagctatga gctgacgtcg ggcctgctcg
     1201 gcctgccggc gaccgccggc cgcgtggaca agatccaggg cagctacggg cggcaggcgc
     1261 tcaaggacgg cctcacgctg caggacgtgt cgggattcaa cccgtacaag ttcggcttcg
     1321 gcgcggcgtc cgactcccac aacacgggct cgccgtaccg gcaggacaac ttcttcggcg
     1381 gccacgcgca gctcgacggc gacgtgcagc ggcgcatggc cggcgtcctc gcgttcggca
     1441 cgctggacgt acgcctggag aatcccgcgg gcctcaccgg cgtgtgggcc gaggaaaaca
     1501 cgcgcgcctc gatctgggat gcgatgtacc gcaaggagac cttcggggtc agcggcccgc
     1561 acatcaaggt ccgtttcttc ggcggctgga actacgcgaa gaacgccttc gacgcgaagg
     1621 actgggtgca ccagtcgtac gcgaacggcg taccgatggg cggcgacctg ccgccgatgc
     1681 cagccggcgg caagggcacg gcgccaacgt tcgtcgtctg ggcggtgaag gacccgacct
     1741 cgggcagcct cgaccgcatc caggtgatca agggctggag ccagaacggg cagagcttcg
     1801 agaagatcta cgacgtcgtt tgggcgggcg accgcaagcc cgatccgtgg accgggcgcg
     1861 tgccggccat caagagcacc gtggacctgg cgaaggcaac ctacaccaac gacgagggct
     1921 cgaccgagct caagacggtc tggacggatc ccgacttcga cgcgagccag cacgcgttct
     1981 actacgcgcg cgtgctggag atcccgacgc cgcgctggtc gctgatccag gcggtgaagg
     2041 cggggatccc gccgccggac gtggtgccgc tgacgggaca ggagcgggcc tggtcgtcgc
     2101 cgatctggta cacgccgagt gcggaggctc gcaaggctgc gcccgcaggc atgacggtgg
     2161 cggcactcaa ggcgaagggt gcgactcagc tgaacgacgc gcagttgaag tcgttcatcg
     2221 tgggcaaggc gtactggatc cgcaacaacg tcaccggcga ccagttcagc gtgagctaca
     2281 cggccgacgg cggcagcatc gtctggcacg tcggaaaata cgcgaacacg ccgagcctca
     2341 cgggtgagct gatgcgcaac ggctacttcg gccagaccac tccgtacaag atcgcgaatg
     2401 gcagggtcgt gaccaacttg caggaggcgc cgttctcgat cgccatctac aagctgggcg
     2461 acagctacta cgccgcgcgc agcaacgagt tcgggtatgc gaactacgaa gtcatccccg
     2521 acccgcagtt cgccgtgaac ccgctcatga gcatgttcaa ccagctctcg atcgagctcg
     2581 ggctgaccgc gcagcagcag cgtgagatca agccgatcct caagagcgag ctcacgcagc
     2641 tcgaggcgct caagaaagac acgtcgatga gcgccgagca gaagctcgag aagctgaagg
     2701 cggcgagcgc cgccatcgac gcgcagatca ccccactcct caacgccgag cagcagccga
     2761 agttccaggc ggtgcgcgac gagatgcggc ggcatctact ggaaacgatg gcgggcaagg
     2821 ccgggaaggc gctcgagagc aagatcgagt cgcacatgta gccgcggggc ctggccgggc
     2881 ctcgcgcccg gccaggnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnttca
     3001 cgatcctggg gtggcaggga atgttgattc cgcccggggt cgtgacgttg accgtgtact
     3061 cgacgtctgc cttgtcctcg cacttgttgt ctatcttcgc ggacttgccg tccccgatga
     3121 catcggcctt catctgtgcg ctgcccttcc cgacaaggtc gacgctcgtg atcgtgtatc
     3181 cctcgggcgc ggcgagcgtg atcttgaccg gagccgcacc gtctcgtacg cggttctcgc
     3241 atttcccgtg gcgatcggtg ccgccggtga actggaaccg gttcgacgaa tcgacggtga
     3301 gggtaacctt gtgctgaccg tttggcatga cgctctcctt ggttctgtcg ttgattccgt
     3361 atcgttcccc tggcccggcg ggcaagcgga ccgcgggctg caccaaggat ccgcgcgacg
     3421 ctggaccgcc ctgcagcgct gatcatagcc ggaccgcgcg cgcccggcgt agccgcatac
     3481 cggacggcgc aggtctcggg ttacctgtat gcgccggaac gtgtgtaggc cgggtcggac
     3541 cgcggatcgc ggtagcccgc cgcttccagc tgggccgcga tggcatccgc ccgtgccgca
     3601 tcgccgaggt cgatggccag caggcgccgt accgcggcaa gcgacaggtt ggtggtgggc
     3661 ttgtcatcca gccgctcgac ctccgcctgc cagacggcac gcgcgcccgt gaggtccccg
     3721 gccgcagcca gagcactgcc cagcgtctcc tcgactctgg cctcgatcag cgtgaactcc
     3781 gggatcatgg tcttgccacg ccgggccgcc tgccagtccc tgtgagtctg tcgtgcggct
     3841 cgcaggctcg cctgcggttc gccctggtgc agcagcacga tgtcacgaag gttgcgcacg
     3901 cggaactcga gccggatgta gtccttgtcc gatgcatcgg ccgaggaggc cccgcccagc
     3961 gtcgattcga tgcgctgcag gctggcgcgt tcctgcgccg tccactcgcc cggaggaatc
     4021 aggccgagta gaacagtctc ggcggtgagc gcgttgtagc gccaagtggt gttggtggga
     4081 tcgttctcga gcaatgtccg gtacacggcc agcgactccc cgagcgcgcg cttcgcctcg
     4141 gccacgttgc cccggttgat atccgcgtcc gcctgcagga tcagcaaccg gcagtagtta
     4201 tacgaggcgc gctggtcctg tgccttcacg tggaacggcc gcatggcctg gaccgccatc
     4261 gcgaattcgt cccgggcgtc ggcgaggcgc cccagctcga gcagggtcgt gccgatccac
     4321 gagcgagtgt cggccaggtt gaacgccagg tcgtaatcgt ccgggctttt cgtcgccagc
     4381 aatccggcca gggcgacgga cctcctgaat tcctccagcg caacctcggg ccggccttgc
     4441 gccagcgcca cgctgcctag attgctgtag gcatacgaca gttccagcag gtaagcgtcg
     4501 ttgtccggag cgcgccgggc gagttcgcgg ctgtaatcca tgtatcgctg catcgactcg
     4561 aaggcgccac cgagg
//
LOCUS       DAOMFD010000160         4538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8232_length_4538_cov_35.353781, whole genome shotgun sequence.
ACCESSION   DAOMFD010000160 DAOMFD010000000
VERSION     DAOMFD010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4538
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8232_length_4538_cov_35.353781"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(93..1643)
                     /locus_tag="PL152_08665"
     CDS             complement(93..1643)
                     /locus_tag="PL152_08665"
                     /inference="COORDINATES: protein motif:HMM:NF025479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4286 family protein"
                     /protein_id="HQR49396.1"
                     /translation="MSEILPSAGNVIYEVNLEADAAIEGPFDTWLRDHIADLLQLPGF
                     LSAEVLADAAPREGRVRRTVQYRLKDQAALDDYLANHAPRMRAAGVALFGDRYTAERR
                     VLAHREEFIRGKVSTENCLNCGEVLTGQHCAHCGQRARVRVLSLWGMTRDLLDDVLDW
                     DARIWRTLRPLAFKPGKLTQDYLRGRRASYTPPFRMYFLLSVAFFVVATAGENPGSSL
                     HFNLDEKGGASLTIEPDDAKGGAQAPPSASGSQPRAAPASPTLTLPAAPPLPPAPGTS
                     GSAKASGKAAAAAPTRTLDAETLRLVDSLVNRVDEKEREKVRQELRDRLATMPAADFA
                     KMKQMMADPCSSKDIHFDLGPYGKQYEPKLRQACSKIWADTPSYGKALFQNIPKMMFI
                     FLPLIAVVMYVLYIGSGRYYVEHLLFVVHFHAFFFLGGLAFLLIERLQWLAPGTAFAD
                     GVKAVAGFFMVAFLVYVPVYLFVAMRRVYGQGRFVTFLKYSTLGVAYLFCLVFTAVGL
                     LFYTALTL"
     gene            complement(1689..3674)
                     /locus_tag="PL152_08670"
     CDS             complement(1689..3674)
                     /locus_tag="PL152_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HQR49397.1"
                     /translation="MTGDKQRRIRDVVFGPPRDPLAPETRKHIALAAILAWIGIGADG
                     LSSSAYGPAEAFMALGRYSDLALYLAIATAFTVFLISLAYNQVIELFPNGGGGYKVAS
                     RLIGPYAGLVAGSALIVDYVLTIAISVASGVDALFSLLPPVHQAVKLEVEVAILVILI
                     YLNLRGARESIKVLAPIFLGFVVTHLALIVYGIANHAQEVHAEISQSLRETSEFAGQA
                     GWFVVIAVLLKAYSLGGGTYTGIEAVSNNVNMLKEPRVRTGKWTMFLMALSLSITAGG
                     IILLYLLWDVAPVQTHETLNAQVFREIIASLGYGGTTSHTLLVATLAFEGALLFVAAN
                     TGFLGGPAVLANMAVDRWAPNQFSALSSRLVTRNGVLLMGVAALAILLWTRGYVSLLV
                     VLYTVNVFITFTLSLAGLTRFWWKQRRDVEGARRRLGLSAFALLVTSGILVIIVVERF
                     LQGGIITLAITSCVIVTGLAIRRHYARVRRMIDAIGRGRRWQIKPLESPPPAIDPAAP
                     TAIFLVSANRNLGVQTISRVETLLPGHFRNHVFLSVGTVDTESYGSDQALRTLQYETR
                     STLDALVNYSNHHGKAACWYDGYASDRRAELERLTLELHATYPNSVFFSSRVVLETDH
                     WWNRWLHSQTPLAMQKVLNEHGLELVILPVRLRESAA"
     gene            complement(3730..>4538)
                     /locus_tag="PL152_08675"
     CDS             complement(3730..>4538)
                     /locus_tag="PL152_08675"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HQR49398.1"
                     /translation="NDCAFPTLNRIVATDDAKVAFKDCQVALLVGARPRGPGMERKDL
                     LLANAQIFSAQGKALDAVADRNVRVLVVGNPANTNSLIAQRNAPGLNARNFTAMVRLD
                     HNRALSQLAEKTGTHTTDLRKVIIWGNHSATQYPDLHHATVKGKPALSLVDDAWYKGE
                     FIPTVQQRGAAIIKARGASSAASAASAAIDHIHTWVKGTPDGDWVSMGVPSDGSYGIP
                     AGVIYGYPVTCKGGDYQIVQGLAVNEFSRGKMDATHKELLEERDGVKDLI"
BASE COUNT          739 a         1467 c         1622 g          710 t
ORIGIN      
        1 cgggtcgtcg cgctgctcca ccgacgcgta gaacgcgtcc atgtcgacgt gcaggatcca
       61 gcgctcggcg gccatgcgga cccgtgacac cttcagaggg tcagtgccgt gtagaacagc
      121 aggccgaccg cggtgaacac gaggcagaag agataggcga cgccgagtgt cgagtacttg
      181 aggaacgtca cgaacctgcc ctggccgtac acgcgacgca tcgcgacgaa caggtacacg
      241 ggcacgtaga ccaggaaagc gaccatgaag aatccggcca ccgccttgac gccgtcggcg
      301 aacgccgtgc ccggtgccag ccactgcaat cgctcgatga gcaggaaggc gaggccgccc
      361 aggaagaaga atgcgtggaa gtgcacgacg aacagcaggt gctccacgta atagcgtccc
      421 gatccgatgt agagcacgta catgaccacc gcgatcagcg gcaggaagat gaacatcatc
      481 ttcgggatgt tctggaacag cgccttgccg tagctcggcg tatcggccca gatcttcgag
      541 cacgcctggc gcaacttcgg ctcgtactgc ttgccgtacg ggccgaggtc gaagtggatg
      601 tccttgctgc tgcacgggtc ggccatcatc tgcttcatct tggcgaagtc cgccgccggc
      661 atcgtcgcca ggcggtcgcg cagctcctga cggacctttt cccgttcctt ctcgtccact
      721 cgattgacca gcgaatcgac gagccgcagc gtctcggcgt cgagtgtccg ggtcggggcc
      781 gcagccgcgg ccttgccgga tgctttcgcc gatccagagg tgccgggtgc tggtggcagt
      841 ggaggcgccg cgggcaaggt cagcgtcggc gacgcgggcg ccgcccgggg ctgcgaaccg
      901 ctggccgacg gcggggcttg cgcgccgccc tttgcatcgt cgggctcgat cgtcaggctg
      961 gcgccgccct tctcgtccag gttgaagtgc agggacgagc ccgggttctc gcccgccgtg
     1021 gcgacgacga agaacgcgac gctgagcagg aagtacatgc ggaacggcgg tgtatagctc
     1081 gcgcgcctgc cgcgcaggta gtcctgcgtg agcttgcccg gcttgaacgc cagcggtcgc
     1141 agcgtgcgcc agatgcgggc gtcccagtcg agcacgtcgt cgagcaggtc gcgggtcatg
     1201 ccccacagcg aaagcacccg cacgcgggcg cgctgcccgc agtgcgcgca gtgctggccg
     1261 gtgaggacct cgccgcagtt aaggcaattc tcggtcgaga ccttgccccg gatgaattcc
     1321 tcgcggtgcg cgagcacgcg tcgctccgcg gtgtagcggt cgccgaacag cgcgacgccg
     1381 gccgcccgca tgcgcggcgc gtggttcgca aggtagtcgt cgagcgcggc ctggtccttc
     1441 agccggtact ggaccgtgcg gcggacgcgt ccctcgcgcg gcgcggcatc ggcgagcact
     1501 tccgccgaga ggaagcccgg cagctgcagc aggtcggcga tgtgatcgcg cagccacgtg
     1561 tcgaaggggc cctcgatggc cgcatcggct tcgaggttca cctcgtagat gacgttgccg
     1621 gcactcggca ggatctcgct cacggcggct agggtaccaa aggccggcgg acgcgagtcg
     1681 ggacctggtc aggcggcgct ctcgcgcagc cgcaccggca ggatcaccag ttcgaggccg
     1741 tgctcgttca gcaccttctg catcgcgagc ggcgtctggc tgtgcagcca gcggttccac
     1801 cagtggtcgg tctcgagcac gacgcggctc gagaagaaca cgctgttcgg gtacgtcgcg
     1861 tgcagctcga gggtgaggcg ctcgagctcg gcgcgccggt cgctcgcgta gccgtcgtac
     1921 cagcaggcgg ccttgccgtg gtggttcgag tagttcacca gcgcgtcgag ggtcgagcgc
     1981 gtctcgtact gcagcgtgcg cagcgcctgg tcgctgccgt acgactcggt gtccaccgtg
     2041 ccgacgctga ggaagacgtg gttgcggaaa tgaccgggca gcagggtctc gacgcgggag
     2101 atggtctgca cgccgaggtt gcggttggca ctgacgagga agatcgccgt cggcgccgcc
     2161 gggtcgatcg cgggcggcgg cgactcgagc ggcttgatct gccagcgccg gccgcgcccg
     2221 atggcgtcga tcatgcggcg cacgcgcgcg tagtggcggc ggatggcaag cccggtgacg
     2281 atcacgcacg aggtgatcgc gagggtgatg atgccgccct gcaggaagcg ctcgacgacg
     2341 atgatcacca ggatcccgga ggtgacgagg agcgcgaagg ccgacaggcc gagccgacgg
     2401 cgagcgccct cgacgtcgcg gcgctgcttc caccagaacc gggtcagacc cgcgagcgag
     2461 agcgtgaagg tgatgaacac gttcacggtg tagagcacca ccagcaggct cacgtagccg
     2521 cgcgtccaca gcaggatggc gagcgcggcg acgcccatca gcagcacgcc gttgcgggtc
     2581 acgaggcgcg acgacagcgc gctgaactgg ttcggggccc accggtccac cgccatgttc
     2641 gcgagcacgg cggggccgcc gaggaagccc gtgttggcgg cgacgaacag cagcgcgccc
     2701 tcgaacgcca gcgtcgcaac cagcagcgtg tgcgacgtgg tcccgccgta gcccaggctg
     2761 gcaatgatct cgcggaacac ctgcgcgttg agggtctcgt gcgtctgcac cggtgcgacg
     2821 tcccacagca ggtagagcag gatgatgccc cccgcggtga tcgacagcga cagcgccatc
     2881 aggaacatcg tccacttgcc ggtgcggacg cgcggctcct tgagcatgtt gacgttgttc
     2941 gagacggcct cgatgccggt gtaggtgccg ccgcccagcg agtacgcctt gagcagcacc
     3001 gcgatcacca cgaaccagcc ggcctggccg gcgaactcgg aggtctctcg caacgactgc
     3061 gaaatctccg cgtgcacctc ctgggcgtgg ttcgcgatgc cgtagacgat gagcgcgagg
     3121 tgcgtgacga cgaagccgag gaagatcggc gcgaggacct tgatcgattc gcgcgcgccg
     3181 cgcaggttca ggtagatgag gatcaccagg atggcgacct cgacctcgag cttgaccgcc
     3241 tggtgcaccg gcggcagcag gctgaacaat gcgtccacgc cgctcgcgac cgagatcgcg
     3301 atcgtgagca cgtagtccac gatcagcgcc gaaccggcga cgagacctgc atatggaccg
     3361 atcaggcggg aggcgacctt gtagccgccg ccgccgttcg ggaacagctc gatgacctgg
     3421 ttgtaggcga gcgagatcag gaagacggta aatgcggtcg cgatggccag gtacagcgcc
     3481 aggtcgctgt agcggccgag ggccatgaac gcctcggccg gaccgtacgc ggacgacgac
     3541 agcccgtcgg cgccgatgcc gatccaggcc aggatcgcgg cgagcgcgat gtgcttgcgc
     3601 gtctccggtg ccagcgggtc gcgcggcggt ccgaagacca cgtcgcggat gcggcgctgt
     3661 ttgtcgccgg tcatggtgct gggtggcgac cgggcggcgc gtcccctgcg cggccgcccg
     3721 gctgatcgat cagatcaggt ccttgacccc gtcgcgctcc tcgaggagtt ccttgtgggt
     3781 cgcgtccatc ttgccgcggc tgaactcgtt caccgcgagc ccctgcacga tctggtagtc
     3841 gccgcctttg caggtgaccg ggtagccgta aatgacgcca gcgggtatgc catagctgcc
     3901 gtcggagggc acgcccatgc tgacccagtc gccgtccggg gttcccttga cccaggtgtg
     3961 gatgtggtcg atcgccgcgg aagccgccga tgccgccgac gaggcaccac gggccttgat
     4021 gatcgcggcg ccgcgctgct gcaccgtcgg gatgaactcg cccttgtacc aggcgtcgtc
     4081 cacgagcgag agagcgggct tgcccttcac cgtcgcgtgg tgcaggtccg ggtactgcgt
     4141 ggccgagtga ttgccccaga tgatgacctt gcgcaggtcg gtggtgtggg ttccggtctt
     4201 ctcggcgagc tgcgacagcg cgcgattgtg gtcgaggcgc accatcgcgg tgaagttgcg
     4261 ggcattgagt cccggtgcgt tgcgctgtgc gatgagcgag ttggtattgg ccgggttgcc
     4321 gaccacgagc actcgcacgt tgcggtccgc caccgcgtcg agagccttgc cctgcgcgga
     4381 gaagatctgc gcgttcgcga ggagcaggtc cttgcgctcc atgcccgggc cgcgcggccg
     4441 cgcgccgacg agcagcgcga cctggcagtc cttgaaggcc accttggcgt cgtccgtcgc
     4501 gacgatgcgg ttcagcgtcg ggaacgcgca gtcgttca
//
LOCUS       DAOMFD010000161         4465 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8454_length_4465_cov_29.874150, whole genome shotgun sequence.
ACCESSION   DAOMFD010000161 DAOMFD010000000
VERSION     DAOMFD010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4465)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4465
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8454_length_4465_cov_29.874150"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1500
                     /locus_tag="PL152_08680"
     CDS             43..1500
                     /locus_tag="PL152_08680"
                     /inference="COORDINATES: protein motif:HMM:NF005045.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HQR49399.1"
                     /translation="MEFLALLSVPLAGALVFGFYGARRWAAEANVGFSLATFVAACVL
                     TARVLREGNLVAAGEQFFIDPFNVFLVVLTAFVGFTTAVFSRPYMRIEAEHGRIGGGR
                     LRLYHSMYQLFMATMLIALTTNNLGLLWVAMEAATLSTVLLVTLYRTPASLEAGWKYF
                     ILCGVGIAQALFGTILLYFAAEKVLGAHGVDALLWTHLAAVKSELEPRVLSLAFVFLL
                     VGYGTKVGLAPLHNWLPDAHAEGPTPISAVLSGLLLNVAIYAVVRCKVLVEGSLQSNL
                     PSQMLMGFGLLSVVLAAFFLWRQRDIKRLFAYSSIEHMGIVTFAFGMGGAVANFAALL
                     HMTVHSLTKSSIFFAVGHASQKVGSQRIDDIRGLITLNPTIGWGLMIGSIAILGLPPF
                     GVFASEFLVLTTAMRQQPWATPILLMALGVAFAAIFSRVQSMVFGETKARRLPHQPAL
                     LPVFAHLAIVLLLGLYIPPYLADWYRAAARLIGGG"
     gene            1565..3046
                     /locus_tag="PL152_08685"
     CDS             1565..3046
                     /locus_tag="PL152_08685"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49400.1"
                     /translation="MSVDDQAWRGVALDVSASGGRLLALWADTTAGSPAAIDALYLLQ
                     HGALVVELPVVDTAAPYSGLHDVFPSAGRLQRAIRDMHGLCSTDADDRPWLRHAAWPA
                     DFHPLAGTSGAEPATTVPPLVDDYHFVRVEGDGVHEVPVGPVHAGIIEPGHFRFSIVG
                     EKVLRLEERLGYVHKGIERRFQSLKLREGHRLAARVSGDSGVAYSWAYCQALEGLSQA
                     VVPPRASWLRGIALELERIANHLGDLGALGNDAGFAVGLAQFMRLKELLLRTTERALG
                     QRYLLDFVVPGGTSRDVDREAAAALSARVRSLAVEVAELRDIYDEHAGLRDRFAGAGV
                     VQPELAARLGLIGLAGRASGQALDLRADLPCEPYSGLGVRKCVLSDGDVLARVAVRFE
                     ELQESCRLVGRLLATLPAGPHSGEVAEPSSDSAAIGLIEGWRGPVVVALTAGEGGSIR
                     RCHPQDPSWHNWPVLEHAIIGNIVPDFPLINKSFNLSYSGHDL"
     gene            3051..3584
                     /locus_tag="PL152_08690"
     CDS             3051..3584
                     /locus_tag="PL152_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49401.1"
                     /translation="MLKTLKHIATVGIVTEAPPAADHELQVRVALERRIREVLGRALC
                     IREVDAGSCNGCELEIHALNNPLYSIESLGIRFVASPRHADMLLVTGPVSRHMEVALR
                     RTYEATPDPKLVVAVGDCGACGGVFGESYASRGAVANVIPVDVVVPGCPPSPTRILEG
                     ILAALADGRAVPDSKGG"
     gene            complement(3581..3841)
                     /locus_tag="PL152_08695"
     CDS             complement(3581..3841)
                     /locus_tag="PL152_08695"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HQR49402.1"
                     /translation="MITASQLRAARAMLGIDQKQLADRARLSLPTIQRMEASDGVIRG
                     NVDSLTKVVQALEDLGIELIGDGAPSVGGGRGVRMRDRAVRG"
     gene            complement(3941..4378)
                     /locus_tag="PL152_08700"
     CDS             complement(3941..4378)
                     /locus_tag="PL152_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49403.1"
                     /translation="MRYRRVNKLSPEDAAYVAGLIDGEGTVTLSRRHATDRRQLVVSI
                     SSTEAEILKWVLATVGTGKITRKSATSPRHAPSLTYSISNRQALALLEQTARFLRSYK
                     RIRAAMVVDQYVRLTPRNGRYTKEVEAARAAFESQLLATKARP"
BASE COUNT          630 a         1460 c         1574 g          801 t
ORIGIN      
        1 ctcgacctgc accacctcga gtcgctgcgg gaggaccgtt aggtggagtt cctggccctc
       61 ctgtcggtac cgctcgccgg ggcactggtc ttcggcttct acggggcgcg ccgctgggcc
      121 gcggaagcca acgtgggctt cagcctggcc acgttcgtcg ccgcgtgcgt gctgactgcc
      181 cgggtgctgc gcgagggcaa cctggtcgcc gcgggcgagc agttcttcat cgatccgttc
      241 aacgtgttcc tggtggtact cacggcgttc gtcggattca cgacggccgt gttctcgcgg
      301 ccgtacatgc gcatcgaggc cgagcatggc cgcatcggcg gcggccgcct gcgcctgtac
      361 cacagcatgt accagctgtt catggcgacc atgctgatcg cgctcaccac caataacctc
      421 gggctgctgt gggtggcgat ggaagccgcg acgctgtcga cggtgctgct cgttacgctc
      481 tatcgcacgc cggccagcct cgaggccggg tggaagtact tcatcctgtg cggcgtgggc
      541 atcgcgcagg cgctgttcgg gacgatcctg ctgtacttcg ccgccgagaa ggtgctcggc
      601 gcacacggcg tcgacgcgct gctgtggacg cacctggccg cggtgaaatc ggagctcgag
      661 ccgcgcgtgc tgagccttgc gttcgtgttc ctgctggtcg gctacggaac caaggtgggc
      721 ctcgccccgc tgcataactg gctgcccgac gcccacgccg agggcccgac cccgatttcc
      781 gcggtgctgt ccgggttgct gctgaacgtc gcgatctacg cggtcgtgcg ctgcaaggtg
      841 ctcgtcgagg gatcgctgca gagcaatctc ccgtcgcaga tgctgatggg cttcgggctg
      901 ctgtcggtcg tgcttgcggc cttcttcctc tggcggcagc gcgacatcaa gcggttgttc
      961 gcatactcgt cgatcgagca catggggatc gtcacgttcg ctttcgggat gggcggcgcg
     1021 gtggccaatt tcgcggcact gctgcacatg acggtccatt cgctcaccaa gtcgtcgatc
     1081 ttcttcgccg tcggccacgc gtcgcagaag gtcgggtcgc agcgcatcga cgacatccgc
     1141 gggctcatca cgctcaaccc caccatcggg tggggactga tgatcggttc gatcgcgatc
     1201 ctggggttgc cgccgttcgg cgtcttcgcc agcgagttcc tggtcctcac gaccgcgatg
     1261 cggcagcagc cctgggcgac accgatcctg ctgatggcgc tcggagtcgc gttcgcggcg
     1321 atcttcagcc gggtgcagtc catggtgttc ggcgagacga aggcgcgtcg actgccgcac
     1381 cagccggcac tgttgccggt gttcgcgcac ctcgcgatcg tgctgctgct ggggctctac
     1441 atcccgccgt atctcgcgga ctggtaccgc gccgccgcgc ggctcatcgg gggcggctga
     1501 gatgccggtc gcacggtggt ggaattcggc gagtcccgtg gccggcgcgc ctggcagccg
     1561 ggcgatgtcc gtcgacgacc aggcctggcg tggcgtggcg ctggacgtat cggcgagcgg
     1621 cggccgcctg ctcgcgttgt gggcggacac gacggccggg agtccggccg cgatcgacgc
     1681 gttgtacctg ctgcagcacg gagcgctcgt cgtggaactg cccgtggtcg acaccgcggc
     1741 cccatattct ggcttgcacg acgtattccc gtcggcggga cggctgcagc gcgcgatccg
     1801 cgacatgcat ggcctgtgct ccacggacgc ggacgatcgg ccgtggctgc gtcacgccgc
     1861 atggccggcg gattttcatc ccctggctgg cacgtccggg gccgaacctg cgacaacagt
     1921 tccaccgctg gtcgacgact atcatttcgt gcgcgtcgag ggcgacgggg tccacgaggt
     1981 ccccgtcggg ccggtgcacg cgggcatcat cgagcccggg catttccgct tctccatcgt
     2041 cggcgagaag gtgctgcggc tggaggaacg tctcggctac gtgcacaagg gcatcgagcg
     2101 acgcttccag tcgctcaagc tgcgcgaggg gcaccggctc gcggcgcgcg tctcgggcga
     2161 ctcgggcgtg gcgtactcct gggcctactg ccaggccctg gaagggctgt cgcaggccgt
     2221 cgttccgcca cgcgcgagct ggctcagggg catcgcactc gaactcgaac gcatcgccaa
     2281 tcacctcggg gacctcggcg cgctcggcaa cgacgccgga ttcgccgtcg ggctggcgca
     2341 gttcatgcgg ctcaaggaac tcctgctcag gaccacggag cgggcgctgg ggcagcgcta
     2401 cctgctggac ttcgtcgtgc ccgggggtac gagccgcgac gtcgatcggg aagccgccgc
     2461 ggcgctctcg gcacgtgtcc gctccctggc cgtggaagtg gccgagctgc gcgacatcta
     2521 cgacgagcac gcgggactgc gcgatcgatt tgcgggcgcg ggcgtggtgc agccggagct
     2581 cgctgcgagg ctgggcctga tcgggctggc cgggcgggcg agcgggcagg ccctggacct
     2641 gcgcgcggac ctgccgtgcg agccgtattc cgggctcggc gtgcgcaagt gcgtcctctc
     2701 ggacggggac gtgctggctc gcgtcgccgt gcgattcgag gagttgcagg aatcctgccg
     2761 gctcgtcggt cggctgctgg cgacgctgcc cgcggggccg cattcaggtg aggtcgcgga
     2821 accatcgtcc gacagcgctg cgatcggact gatcgaaggc tggcgtggcc cggtggtcgt
     2881 ggcgctgacc gcgggcgagg gcggttccat ccgccgctgc catccgcagg atccctcgtg
     2941 gcacaactgg ccggtgctgg agcacgccat catcggcaac atcgtgccgg atttcccgct
     3001 catcaataag tcgttcaacc tgtcgtacag cgggcacgac ctgtagcctc atgctcaaga
     3061 cgctgaagca catcgccacc gtcggcatcg tgaccgaggc tccgcccgcg gccgaccacg
     3121 aactgcaggt acgcgtggcg ctcgagcgtc gcatccgcga ggtgctgggg cgggcgctct
     3181 gcatccggga agtcgacgcg ggttcgtgca acggttgcga actcgagatc cacgcgctca
     3241 acaacccgct gtacagcatc gagtcgctgg gcatcaggtt cgtggcgagc ccgcgtcacg
     3301 cggacatgct gctcgtgacc ggaccagtgt cgcggcacat ggaagtggcc ctgcgtcgca
     3361 cgtacgaggc gacacccgac ccgaagctcg tggtcgccgt cggggactgc ggtgcgtgcg
     3421 gcggagtgtt cggggagagc tacgcgagcc gcggtgcggt cgcgaacgtg ataccggtgg
     3481 acgtcgtggt gccgggctgt ccgccgtcac cgacgcgcat cctggagggc atcctcgccg
     3541 cgctcgcgga tggacgcgcg gtcccggatt cgaagggagg ctagccgcgc acggcgcgat
     3601 cgcgcatgcg cacgccgcgt ccgccgccca cgctcggcgc gccgtcgccg atcagttcga
     3661 tgccgaggtc ctcgagcgcc tgcacgacct tggtcagcga atccacgttg ccgcggatga
     3721 ctccgtcgct ggcttccatg cgctggatcg tcggcaacga cagccgtgca cgatcggcga
     3781 gctgcttctg gtcgattccg agcatggccc gggccgcgcg cagttggctg gcagtaatca
     3841 tgtttcagcc atgaacaacg aagcctggaa gagaagaatg caggcgggag atgttagtcc
     3901 cgtccgtggg ctgacgccac cggcagcagt ggcgtccgtt ctacgggcgc gccttggtgg
     3961 cgagcagctg tgattcgaaa gctgcccgcg ccgcttcgac ttccttggta taccggccgt
     4021 tgcgcggagt cagccggaca tattgatcga cgaccattgc agccctgatt cgcttgtacg
     4081 accgcaggaa ccgggcggtc tgctccagca gtgccagcgc ctgacggttc gaaatcgaat
     4141 atgtcaggct tggtgcatgc cgtggcgacg ttgctgactt acgggtgatc tttccagtcc
     4201 cgacagtcgc caggacccat ttgagtattt ccgcttcggt gctgctgatg gaaacgacga
     4261 gctggcgccg atccgtcgcg tgacgacggg acagcgtcac ggtgccttcc ccatcaatga
     4321 ggcccgcgac gtatgcggca tcttccgggc tgagtttgtt tacgcggcga tagcgcatcg
     4381 ggacgcacgt cgggcgcata aagaccctcc ggcccgaagg ccggagggtc tgggatggtg
     4441 gaggcggcgg gacagctggt ctggc
//
LOCUS       DAOMFD010000162         4426 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8576_length_4426_cov_24.733928, whole genome shotgun sequence.
ACCESSION   DAOMFD010000162 DAOMFD010000000
VERSION     DAOMFD010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4426)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4426
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8576_length_4426_cov_24.733928"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(168..1166)
                     /locus_tag="PL152_08705"
     CDS             complement(168..1166)
                     /locus_tag="PL152_08705"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF007989.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HQR49404.1"
                     /translation="MTVRNGFVDTVGHTPLIRLRSFSDETGCEILGKAEFLNPGGSVK
                     DRAARGILEDFERRRLLKAGGTVVEGTAGNTGIGFAHLCNARGYRCVIVMPDNQSAEK
                     AVLLRALGAEVQIVKTVPYADDNHYQKLAPRVAAAIDGAVCGNQFDNTANRDAHYGST
                     GPEIWHETGGRIDAFTCATGTGGTLGGVSRYLKEQNPRVRIVLADPLGSALYHWVKTG
                     TPRTEGSSSVTEGIGIGRVTANLADSPIDDALQVDDAEIVRTGYRLLHEEGLFLGGTS
                     GVNVAAAVRVARDLGPGHTVVTVLCDGGQKYLSRFWSREYLESKGLAKHAVARSIR"
     gene            1270..2244
                     /gene="cysB"
                     /locus_tag="PL152_08710"
     CDS             1270..2244
                     /gene="cysB"
                     /locus_tag="PL152_08710"
                     /inference="COORDINATES: protein motif:HMM:NF009326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HQR49405.1"
                     /translation="MKLHQLRYLAAVAQSGLNITAAAEKLHTSQPGVSKQIKLLEDEL
                     GFQIFVRDGRNLSRVTPAGQQVIDRAVRILREVQNIKRLSDDFKDEARGSLSIATTHT
                     QARYVLPAVVTQFRARYPDVRLHLHQGTSEQIGEMAELDRIDFAIATGSQSLFEGYTL
                     LPCYRWHRHVVVPRGHPLAKVKSPTLKQLAAHPIITYVFSFSGPSSLHEIFAREGLVP
                     NVVLTARDADVIKTYVRLGLGIGIVASMAIDAKEDADLVEIDTSHLFPAHVTWIGFRR
                     GGLLRKYQLDFMQLFAPHLPRRTIERVVSAGTQADVDELVAGIDLPVR"
     gene            complement(2312..3454)
                     /locus_tag="PL152_08715"
     CDS             complement(2312..3454)
                     /locus_tag="PL152_08715"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HQR49406.1"
                     /translation="MSGRRTSFGFYTVIAALTVAVPQIEARAATATVIATGLVNPKGI
                     DESPSGALYVAEAGNGGSDCSSIAGACFGLSGAVSRIDPTGVQPAVRIVTGLPSIYSG
                     SPRVAVGANDVSFSRYGAPTIIMGLGGDPGVREKLGAAGANLGKLLRILGDNRTMAVA
                     DISAFEQANNPDRTTIDSNPYGIEAQPSEWAVADAGANTLLRARPNGKTSLLAVFAPV
                     TVTNPLNGQTVTAQAVPTSVTRGPDGYWYVGTLTGFPFATGAASVWRVPPEGGKPELC
                     ASGFTTIADVAFDPYGRLYVLEISSGLRVPPVPPPPPLRTPGKLKRLDACGTAPVTVY
                     DNSDPTQGVVLEYPAGVVIGLDGAAYVTNRTVDSGGGQVIRIALTD"
     gene            3603..4250
                     /locus_tag="PL152_08720"
     CDS             3603..4250
                     /locus_tag="PL152_08720"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR49407.1"
                     /translation="MSTGKRPAGPIANLERGRTRQKLRTRRELLATAGRLIVEGKQPT
                     VAEVADAADVSRRTAYRYFPSQEKLLTEAALERVRPAIEAAVAAAPAGLTEADLDRRL
                     DAFVKSIQALTVENEHLLRTMVHLTVLEQPPPGTRPRGSRRIDWIELAVASLRPRIGR
                     AAYARLVSALSLIVGIEALLVLRDIRGLTETQAVHVSQWMARAVLRETMREARLE"
BASE COUNT          693 a         1558 c         1447 g          728 t
ORIGIN      
        1 ccggcagccc gctgccgacg tggacctcca cgcggaccag cggcgcgtcg aggccctgct
       61 gggtgcgact caggatggtg gctagcggca cggccgtgct ccgatgaacg ggcggccatg
      121 atcgcgacga cgggtgcgcc gccgtcaacg cgcaattcag tcgggagtca cctgatactc
      181 cgagcgaccg cgtgcttcgc gagtcccttg gactccaggt attcgcggct ccagaaccgc
      241 gagaggtatt tctgtccgcc gtcgcaaagg accgtcacga cggtgtgccc gggcccgaga
      301 tcgcgcgcca cgcgcaccgc ggccgcaacg tttacgccgc tcgttccacc gaggaacagg
      361 ccctcttcgt gcagcaggcg gtagcccgtg cgcacgatct ccgcgtcgtc gacctgcagc
      421 gcatcgtcga tcggcgagtc cgcgaggttg gccgtgacac gcccgatgcc gatgccctcg
      481 gtcacggaac tcgagccctc ggttcgcggc gtaccggtct tgacccagtg gtagagcgcg
      541 ctgcccagcg ggtcggcgag cacgatgcgc accctgggat tctgctcctt cagatagcgc
      601 gacacgccgc cgagcgttcc gcccgtcccg gtcgcgcagg tgaacgcatc gatgcgcccg
      661 ccggtctcgt gccagatctc gggcccggtc gagccatagt gcgcgtcgcg gttcgccgta
      721 ttgtcgaact ggttgccgca gacggccccg tcaatcgccg ccgcgaccct cggcgcaagc
      781 ttctggtagt ggttgtcgtc ggcgtacggt acggtcttca cgatctgcac ttcggcaccg
      841 agcgcgcgca gaagcaccgc cttctccgcc gactggttgt ccggcatcac gatcacgcag
      901 cgatagccgc gcgcattgca caggtgcgcg aacccgatgc cggtgttgcc cgccgtgccc
      961 tcgaccaccg tgccaccggc tttcagcagc ctccgtcgtt cgaaatcttc gaggatgccg
     1021 cgggctgcgc ggtccttgac cgagccaccg gggttgagga actcggcttt gccaagtatt
     1081 tcgcagcccg tctcgtcgct gaaactgcgc aagcggatca acggggtgtg gccgacggta
     1141 tcgacgaagc cgttgcggac ggtcatggtg gctccgcggc aagggtgtgc gcaccattct
     1201 atccgcggcg ggccgccggc gacccccagc cttggcatga ggcccgaact ccccggttag
     1261 aatgcgcgca tgaagctgca tcaactcagg tacctggcgg cggtcgcgca gtcgggcctc
     1321 aacatcaccg cggccgcgga gaagctgcac acgtcgcagc cgggggtgtc caagcagatc
     1381 aagctgctcg aggacgagct cggcttccag atcttcgtgc gcgacgggcg caacctgtcg
     1441 cgcgtgacgc cggccggcca gcaggtgatc gatcgcgcgg tgcgcatcct gcgcgaggtg
     1501 cagaacatca agcgcctgtc cgacgacttc aaggacgagg cgcgcggctc gctgtccatc
     1561 gccacgacgc acacacaggc gcgctacgtg ctgccagccg tggtcacgca gttccgggca
     1621 cgctacccgg acgtgcgcct gcatctgcac caggggacct ccgagcagat tggcgagatg
     1681 gccgagctcg accgcatcga cttcgcgatc gccacgggct cgcaatcgct gttcgagggc
     1741 tacacgctgc tgccgtgcta ccggtggcac cgtcacgtcg tggtgccgag gggccacccg
     1801 ctcgcgaagg tgaagtcgcc cacgctgaag cagctggcgg cgcacccgat catcacctat
     1861 gtcttcagtt tcagcggccc atcctcgctg cacgagatct tcgccaggga aggactggtg
     1921 ccgaacgtcg tgctcaccgc gcgcgatgcc gacgtgatca agacctacgt gcgcctcggg
     1981 ctcggcatcg gcatcgtcgc gagcatggcg atcgacgcaa aggaggacgc cgacctcgtc
     2041 gagatcgaca cctcgcacct gttcccggcg cacgtcacgt ggatcgggtt ccgccgcggc
     2101 ggcctgctgc gcaagtacca gctcgacttc atgcagctgt tcgcgccgca cctcccccgg
     2161 cgcacgatcg agcgcgtcgt ctcggccggg acccaggccg acgtggacga actcgtggcg
     2221 ggcatcgacc tgccggtgcg ctaggcggac gctgcggcac gtccctgtgc cgcgagcctt
     2281 tggcccggac ccgggtcttt cgaagctcgg gtcagtcggt caacgcgatg cgaatcacct
     2341 gcccgccgcc cgagtccacg gtccggttgg tgacgtaggc cgcgccgtcg agcccgatca
     2401 cgactccggc cgggtattcg agtaccacgc cctgcgtcgg gtcggagttg tcgtacaccg
     2461 tgacgggcgc cgtaccgcac gcgtccaggc gcttcagctt gcccggcgtc ctgagcggcg
     2521 gcggcggcgg gaccggcggc accctcaggc ccgaggagat ctccagtaca tagagccttc
     2581 cgtacggatc gaacgccacg tccgcgatcg tcgtgaaccc ggacgcgcag agttcgggct
     2641 tgccaccctc cggcggcacc ctccagacac tcgccgcgcc ggtggcgaac gggaagcccg
     2701 tcagcgtccc gacgtaccag tacccgtccg gcccgcgcgt gaccgacgtc ggaacggcct
     2761 gcgccgtcac cgtctggcca ttgagtggat tcgtcaccgt cactggcgcg aacaccgcga
     2821 gcagactggt cttgccgttg ggccgcgcgc gcaacagcgt attggcgccg gcgtcggcca
     2881 ccgcccactc cgaaggctgc gcctcgatcc cgtacgggtt gctgtcaatc gtcgtcctgt
     2941 cgggattatt ggcctgctcg aacgccgaga tatccgccac ggccatcgtg cggttgtcgc
     3001 cgaggatgcg cagcagcttg ccgaggttgg cgccggctgc gcccagcttt tcacgcacgc
     3061 ccgggtcgcc gccgaggccc atgatgatcg tgggtgcccc gtaccgggag aacgacacgt
     3121 cgttcgcacc gaccgctacg cgcggactgc cgctgtagat ggacggcagc ccggtgacga
     3181 ttcgcacggc gggctgcacg ccggtcgggt cgatccggct gacggctccc gacagaccga
     3241 agcatgcgcc cgcaatgctg ctgcagtcgc tgccaccgtt gccggcctcg gccacgtaca
     3301 gtgcgccact tggcgactcg tcgatgccct tcgggttgac gagacccgtg gcgatgaccg
     3361 tggccgtcgc ggcccgcgcc tcgatctgcg gcacggccac agtcagggcc gcgatcacgg
     3421 tgtagaaacc gaaagacgtt ctccgcccgc tcatcttcat tgcctccatt cgtgttggtg
     3481 tccgaccgac cacggcgtcc tggacgcatc gggcgaccgg cttcccacct cacccttgcg
     3541 gcatctatat gccgtaatgg cagtactgtg ccattacgac aagtattgct agaatcgctg
     3601 gcgtgtcaac aggcaagcgc cccgccggcc cgatcgcgaa cctcgagcgc ggcaggacga
     3661 gacagaaact tcgtacgcgc cgcgagctgc ttgcgacggc cggccgactg attgtggaag
     3721 gcaagcaacc gaccgtcgcc gaggttgccg acgcagccga tgtctcgcga cgaactgctt
     3781 accgctactt tccgtcgcag gagaagctgc tcaccgaggc cgcgctggag cgggtgcgcc
     3841 ctgccatcga ggcggccgtc gcggctgcgc cggcgggcct gaccgaggct gacctcgaca
     3901 ggcgcctcga tgcgttcgtg aaatcgatcc aggcgctcac ggtcgagaac gagcacctgc
     3961 ttcgaaccat ggtccacctg acggtgctcg agcagccgcc accgggcacc cgcccgcgcg
     4021 ggtcccgccg catcgactgg atcgagctcg ccgtcgcctc gctccggccg cgcattggca
     4081 gggccgccta tgcccggctc gtctccgcgc tgtccctcat cgtcggcatc gaggcgctgc
     4141 tcgtgctgcg cgacattcgc gggctcacgg agacgcaggc ggtccatgtg agccagtgga
     4201 tggcgcgcgc agtgcttcgc gagacgatgc gtgaggcgcg gctcgagtag gatccgtcac
     4261 tgtcggagtg cgattcgcca gactccggac ccgtcggccg gtatgcggtc aaatgcgagt
     4321 cattcttatt tacactcagg cccttcctgc ctagactccg tatcgacgac acccggagga
     4381 cgcatggaca ggaaggccgt acacgacacg ctcaaccgga tcatgg
//
LOCUS       DAOMFD010000163         4412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8632_length_4412_cov_30.599036, whole genome shotgun sequence.
ACCESSION   DAOMFD010000163 DAOMFD010000000
VERSION     DAOMFD010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4412
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8632_length_4412_cov_30.599036"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            260..730
                     /locus_tag="PL152_08725"
     CDS             260..730
                     /locus_tag="PL152_08725"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR49408.1"
                     /translation="MNFQPFDLAQQPDLMRWFPDAVSVRTWGGPGFRHPFDERTFRED
                     AKFDRLSSWALVATEGSLAAFGQYYLKLGRCHLARLAVSPSLRGRGVGRRLVHELGRV
                     GAAALGASAYSLFVYEGNATALRLYRSLGFAEAPYPERSPEHDGILFMVATTIR"
     gene            786..1178
                     /locus_tag="PL152_08730"
     CDS             786..1178
                     /locus_tag="PL152_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49409.1"
                     /translation="MTGYVKFGCPSCAAVHNGGLVLEYDDSEPQDMTRRSDAPATNAI
                     KGDGDAFPGRAGSGESDQYAGIERRVNPHRDDERADWETIALRRIARRVELMPAAGAT
                     SSSASVDHGWEEAAVNALRRRLGELKSG"
     gene            complement(1257..2420)
                     /locus_tag="PL152_08735"
     CDS             complement(1257..2420)
                     /locus_tag="PL152_08735"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HQR49410.1"
                     /translation="MKLLSDNSRLSGTVAALGAAVLGLGIAPNAASHASPSPAHGSAV
                     ATVYAGGLVNPRGLKFGPDGYLYVAEAGYPVDPTTAAPAGLGGACSTGTDIPGEYAGS
                     TTGSRISRIDSAGHVETVVDGLPSSEVGGLASGVADVAFVGHTLYAILAGAGCSHGVP
                     GIPNGVIRVNADHSWTMIANLTAFQQAHPVAHPTDPITGDFEPDGTWYSMIALRGDLY
                     AIEPNHGELDRITRSGHVSRVADISATQGHVVPTVVANHGVFYVSNLGGFDAEDLNMQ
                     SVYKITPSGQIKVVATGLSKVLGVAFDRRDRMYVLESSYSTSDPFPEPGTGRLIRILP
                     NGKQQVLIDGSSGMLVVPTGMTFGPDGALYISNFGFGAPPIGLGQVLRVEIVN"
     gene            complement(2574..3221)
                     /locus_tag="PL152_08740"
     CDS             complement(2574..3221)
                     /locus_tag="PL152_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR49411.1"
                     /translation="MTTPRQVLDVSIATPDATLSGEAWLPPGAPGIVLFAHGSGSSRM
                     SPRNRAVADAIVRGGIGTLLFDLLTADEESRDRVDGSLRFDIGLLTERLVAATRWLRN
                     QDWAEELTLGYFGASTGAAAALAASVVCPVRAVVSRGGRPDLAWTALPRVRAPTLLIV
                     GSHDPVVLELNQRAQAQLTCESKLEVVRGATHLFEESGALEQVAALATSWFQRWL"
     gene            complement(3228..4001)
                     /locus_tag="PL152_08745"
     CDS             complement(3228..4001)
                     /locus_tag="PL152_08745"
                     /inference="COORDINATES: protein motif:HMM:NF006070.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR49412.1"
                     /translation="MNVKSLFDLTGKVALITGGSRGLGLQMALALGGMGARVAITARK
                     ADELAEAKSRLEAEGIDVLTVTHDLSKLDTIPGLVDGVVKHFGTIDILVNNAGATWGA
                     PAEDYPADAWRKVMTLNVDAMFFLSQYVGKQVMIPKRSGKIINISSVAGLRGNPGGMS
                     TIAYNTSKGADINFTRALAAEWGRYNINVNAICPGFFPSKMSQGLLDALGEKVLAMTP
                     LRRIGGDEDLMGTAVFLASEASRHVSGQYIAVDGGASCC"
     gene            complement(4146..4316)
                     /locus_tag="PL152_08750"
     CDS             complement(4146..4316)
                     /locus_tag="PL152_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49413.1"
                     /translation="MKSSGHAPTHDSTDDHSFDNSHHPIWAIANAVATFFLIAVAISF
                     LVMLTDAFISHT"
BASE COUNT          696 a         1444 c         1544 g          728 t
ORIGIN      
        1 ccgcggtcat ggcgagccac agccggttcc cgatgcgggc ccggtactcg tgtcgcatgc
       61 gtacccccgt ccgttcatga agggtgtcgc cggcaaagtg tccgagtcgc gcgcgatgcc
      121 ggctatccgt agttgttcgc cacccgactg ggagacttcg caccgcttga cgtgtctgcg
      181 cccaggcaga agcattgctt ccttggtcga attccgcagc ggggcagggc ccagttcccg
      241 gccccggacg tgcaatgcga tgaacttcca gccgttcgat cttgcacagc agccggacct
      301 catgcgctgg tttccggacg cggtcagcgt gcggacctgg ggcgggcccg gattccgaca
      361 tccgttcgac gaacgcacgt ttcgcgagga cgcgaagttc gaccgcttgt cgtcgtgggc
      421 actcgtcgcg acggagggaa gtctcgcggc cttcggccag tactacctca aactcgggcg
      481 ctgtcacctc gcgcggctcg ccgtgtcgcc ttcgctacgc ggccgcgggg tcggccgacg
      541 cctggtgcac gagcttggcc gtgtcggcgc ggcggctctc ggagcgagtg cgtactcgct
      601 gttcgtgtac gagggcaatg cgaccgcgct tcggctctat cgatccctgg gtttcgccga
      661 ggcgccgtac ccggaacgct cacccgagca cgatggaatc ctgttcatgg tggcgacgac
      721 gatccgctag gtgcagtcga acggtcgccg gcgcgaagag acgtcgcgcg ggatcgtgct
      781 gttacgtgac aggttacgtc aaattcggct gtccctcttg cgccgcagtg cacaatggtg
      841 gcttagtgct cgaatatgac gactccgagc cgcaagacat gacgcgccga tcggacgctc
      901 ctgccacgaa tgcgattaag ggagatggtg acgcctttcc cggccgagcg ggctccggcg
      961 agtccgacca gtacgccggg atcgaacggc gcgtcaaccc gcaccgggac gatgagcgtg
     1021 ccgattggga gacgatcgcg ctcaggcgga tcgcgcgccg cgtcgagttg atgccggcag
     1081 ccggtgcgac gtcgagttcg gcttcggtgg atcacggctg ggaagaagcc gcggtcaacg
     1141 cactgcgccg gcgactcggc gaactcaagt ccggctagaa ccacgccggg tgtctggggc
     1201 cgccgtcgtc gaccccggcc cgcgacaggc gggccgaggt ccgctgcgac acaaggtcag
     1261 ttcacgatct cgacacgcag gacctggccg agaccgatcg gcggtgcacc gaagccgaag
     1321 ttcgaaatgt agagcgcgcc gtccggaccg aacgtcatgc cggtgggcac gacgagcatg
     1381 ccgctcgagc cgtcgatcag gacctgctgc ttgccgttcg gcagaatccg gatcaggcga
     1441 ccggtgccgg gctcgggaaa cggatcactc gtcgagtagg aactctcgag cacgtacatc
     1501 cgatcgcgcc ggtcgaaggc gacgccgagc accttgctaa gccccgtcgc caccaccttg
     1561 atctggccgc tcggcgtgat cttgtagacg ctctgcatgt tgaggtcctc ggcatcgaaa
     1621 ccaccgaggt tgctcacgta gaagacaccg tggttcgcaa ccacggtcgg caccacgtga
     1681 ccctgcgttg cggaaatgtc ggcgaccctc gacacgtggc cggacctcgt gatccggtcg
     1741 agctcgccgt ggttgggctc gatcgcgtac aggtcgccac gcagcgcgat catgctgtac
     1801 cacgtgccgt cgggttcgaa gtcgccggtg atcggatcgg tcgggtgggc gaccgggtgg
     1861 gcctgctgga atgcagtgag gttggcgatc atggtccagc tgtggtccgc gttcacgcgg
     1921 atgacgccgt tcgggatgcc cggcacgccg tgcgagcacc cggcgccagc gaggatcgcg
     1981 tagagcgtgt ggccaacgaa cgcgacgtcc gcgacgccgc tggccagccc gccgacctcg
     2041 ctcgacggca atccgtcgac gacggtttcg acgtgaccgg ccgaatcgat cctcgagatg
     2101 cgcgagccgg tcgtgctgcc ggcgtactca cccggtatgt cggtgccggt ggagcacgcc
     2161 ccgcccagtc ccgctggagc cgcggtcgtc gggtcgaccg gataaccggc ctcggcgacg
     2221 tagagatatc cgtccgggcc gaacttgagc ccgcgcgggt tgacgagccc gcccgcatac
     2281 accgtcgcca ccgccgagcc atgcgccggg gacggcgagg cgtggctcgc cgcgttcggc
     2341 gcgattccca gtccgagaac agccgcgccg agcgccgcga cggtgccgga aagccgtgaa
     2401 ttgtcgctta gcagtttcac ttggaccccc tgcgttcgat tgaacgggtt gaggacgacg
     2461 gccggggacc gcggtgaccg gtagcccggc cgggggacgc tagcggaatc ctaatcattc
     2521 gcattgtcaa gcccgccgct aacggcgggt gacggccggg ggtcggagcc gcgttacagc
     2581 cagcgctgga accacgaggt ggcgagcgcc gccacctgct cgagcgcgcc cgattcctcg
     2641 aacaggtgcg tcgcgccacg gacgacctcg agcttcgact cgcacgtgag ctgcgcctgc
     2701 gcgcgctggt tcagttccag caccaccgga tcgtgcgagc ccacgatgag cagcgtcggc
     2761 gcgcgcacgc ggggcagggc ggtccacgcc aggtccggcc ggccgccgcg tgatacgacc
     2821 gcacgtaccg ggcacacgac gcttgcggcg agcgcggccg cggcgccggt actggcgccg
     2881 aagtagccca gcgtcaattc ctcggcccag tcctgattgc gcagccagcg cgtcgcagcc
     2941 accagccgtt cggtcagcag tccgatgtcg aagcgcaggc tcccgtccac gcggtcgcgg
     3001 ctctcctcgt cggccgtgag caggtcgaac agcagggtgc cgattccgcc gcggacgatc
     3061 gcgtcggcca cggcccggtt gcgcgggctc atgcggctgc tgccgctgcc gtgcgcgaac
     3121 agcacgatgc cgggcgcgcc gggcggcagc cacgcttcac cggagagggt tgcgtccggt
     3181 gtcgcgatcg agacgtcgag gacttgccgg ggcgtcgtca tcgacgacta gcagcagctc
     3241 gcgccgccgt cgacggcgat gtactggccg ctgacgtggc gcgatgcttc cgaggcgagg
     3301 aacacggccg tgcccatcag gtcctcgtcg ccgccgatgc gtctcagcgg cgtcatggcc
     3361 agcaccttct cgcccagggc gtcgagcagg ccctgcgaca tcttcgacgg gaagaagccc
     3421 gggcagatgg cgttgacgtt gatgttgtag cggccccact cggcggcgag cgcacgggtg
     3481 aagttgatgt cggcgccctt gctcgtgttg taggcgatgg tggacatgcc gcccgggttg
     3541 ccgcgcagcc cggcgaccga cgagatgttg atgatcttgc ccgagcgctt cgggatcatc
     3601 acctgcttgc ccacgtactg gctcaggaag aacatcgcgt cgacgttcag cgtcatcacc
     3661 ttgcgccagg cgtcggcggg atagtcctcc gcgggcgcgc cccaggtggc gccggcgttg
     3721 ttcaccagga tgtcgatcgt gccgaagtgc ttgacgacac cgtccacgag cccggggatg
     3781 gtgtcgagct tcgacaggtc gtgggtgacg gtcagcacgt cgatgccttc cgcttcgagc
     3841 cgcgactttg cctcggccag ctcgtcggcc ttgcgtgcgg tgatcgcgac cctggcgccc
     3901 atgccgccga gcgcgagggc catctgcagg ccgagtccgc gggatccacc ggtgatcagg
     3961 gcgaccttgc cggtcaggtc gaagaggctc ttgacgttca cgttgctggc tccagggctg
     4021 gtcgatgtag cgaggccacc ggcagcctgg ggcggttgcc gggcaggtac cccaaagtca
     4081 cagcgtaccg ggcggctcga gacagtgcca tcgtccgctc gcgaggacga gcggacggcc
     4141 cggagtcagg tgtgcgagat gaacgcgtcg gtcagcatca cgagaaacga gattgcgacc
     4201 gcgatcagga agaacgtcgc gaccgcattg gcgatggccc agatggggtg gtgcgagttg
     4261 tcgaaggagt ggtcatcggt cgagtcgtgt gtcggtgcat ggcctgagga tttcatggcc
     4321 ggtgtccctc cacagcctgg cggacctgtc caagccaaga ctagtccgcc gggtggcggg
     4381 tcgaaagcga ggaatgtcga tgcctggcat cg
//
LOCUS       DAOMFD010000164         4342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8863_length_4342_cov_28.331467, whole genome shotgun sequence.
ACCESSION   DAOMFD010000164 DAOMFD010000000
VERSION     DAOMFD010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8863_length_4342_cov_28.331467"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="PL152_08755"
     CDS             complement(<1..278)
                     /locus_tag="PL152_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49414.1"
                     /translation="MKISIHTESWASARPFRITGREWLTFDPVVVELERDGAIGRGEA
                     YGVFYADETAASMAAELERVADRVAAGIDRAAAQSLLPPGGARNALDCA"
     gene            complement(275..1303)
                     /locus_tag="PL152_08760"
     CDS             complement(275..1303)
                     /locus_tag="PL152_08760"
                     /inference="COORDINATES: protein motif:HMM:NF019375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1611 domain-containing protein"
                     /protein_id="HQR49415.1"
                     /translation="MPELVIRFDSPFVVFLGDITDDTYAKTGLGLAQWRRDDCIGQVR
                     LPGCSVDAGVPDLTPAQAASAGARSLIVGSAAVGGGIPAAWVTTLCEAAAAGLDIVAG
                     LHIRLASLPGLADAAARGRARLIDVRVPPPGLPVGTGRKRTGRRVITVGTDCACGKKY
                     TALALDRELRRRGVASDFRATGQTGIMIAGRGMPIDAVVADFVTGAAEVLSPDNAPGH
                     WDVIEGQGAIFHPGYLQVTAGLLVGSQPDAFVVCHDPLRTHVSGWEDFALPTIAQVIE
                     RTVAIGRITNAAIRCVGISLNTSHVPAHERAALLARHASESGLPCVDPLVEGMGAIAE
                     RLLEEFRA"
     gene            1452..2840
                     /locus_tag="PL152_08765"
     CDS             1452..2840
                     /locus_tag="PL152_08765"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HQR49416.1"
                     /translation="MSRDSDAAFMGHPKGLLYLAFTEAWERFSYYGMTALLVLYMVNQ
                     LLLPGHVEHVAGFAAFRSAIESVFGPLSTQALASQVFGLYSGFVYFTPMLGGLVADRW
                     IGQRAAVVVGALAMSAGHLAMAFDRSFLLALALLVVGSGLLKGNISAQVGALYPVSEE
                     ARRTRGFAIFSTGINFGAVAGPLVCGVLAQAFGWHYGFGLAAVFMLGGLATYLRGYRH
                     LPARVGRRQHSEGRLTANDWRVIAALVAVMVITVFQSIAYYQLANVLPVWIQDHVALD
                     VGSFTVPIPWYQSIDPLASIVFVPVLFWIWKRQAAGRGEPGDLGKIATGAWVCVASNL
                     VLVGAIVAAQGRLVHPVWPFLYCVGQGIGFLYYWPTLLALVSRAAPARVNATMMGFAF
                     MSLFVANTLIGWVGTFYERLGPLRFWMLHAAIAATGGALALALGGAIKRVIDTGADLP
                     LRPSAMTLEVER"
     gene            complement(3081..3869)
                     /locus_tag="PL152_08770"
     CDS             complement(3081..3869)
                     /locus_tag="PL152_08770"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HQR49417.1"
                     /translation="MTLRMCAVALLLSGCSTLPDGSRWGENASFRVGWDRVGDAALEA
                     AKNPWVWAPLAGAAVMQIDHWDTRVSTWAIDNTPVFGSTSNASNASNVLEFSAGAAYV
                     ASVFATPGGAAGPQWWDAKLSGSLVGLGAIAAESVVTEGLKVTVNRTRPNGGSHSFPS
                     GHTSFAAVADTLAADNLEVTPMDDSLRTALVIGTDALTIATGWARVEAGAHYPSDVLV
                     GMSIGNFFGRMFDDAFLGDGLSQRLSVTFDPARGGGELVWNLRF"
BASE COUNT          654 a         1492 c         1477 g          719 t
ORIGIN      
        1 gcgcagtcga gcgcattgcg cgcgccgccc ggcggcagca gcgactgcgc cgcggcgcga
       61 tcgattccag cggcgacacg atcggcgacg cgctccaact ctgctgccat cgaggccgcg
      121 gtctcgtcgg cgtagaagac cccgtacgcc tcgccccggc cgatcgctcc gtcgcgctcg
      181 agctcgacca cgacggggtc gaacgtgagc cactcgcgcc cagtgatgcg aaacggccgc
      241 gcactggccc agctctcggt atggatgctg atcttcatgc ccggaattcc tcgagcagcc
      301 gctcggcgat cgcgcccatg ccctcgacga gcgggtccac gcagggcagt ccgctttccg
      361 aagcgtgacg cgcgagcagc gccgctcgct catgggcagg aacgtgcgac gtattgagcg
      421 aaatgccgac gcaacggatc gcggcgttcg tgattcgccc gatcgcgacc gttcgttcga
      481 tgacctgcgc gatcgtgggc agggcaaagt cctcccagcc ggacacgtgg gtgcgcaacg
      541 gatcgtggca gacgacgaac gcatcgggtt gcgatccgac cagcagtccc gccgtcacct
      601 gcaggtaacc gggatggaag atcgcgccct gcccttcgat cacgtcccag tgacccggtg
      661 cgttgtccgg cgacaggact tctgccgcgc ccgtcacgaa gtccgcgacg accgcgtcga
      721 tcggcatccc gcggccggcg atcatgatcc cggtctgtcc cgtcgcgcgg aaatcgctgg
      781 cgacgccgcg ccggcgcaat tcgcgatcga gggcgagcgc cgtgtacttc tttccacacg
      841 cgcagtcggt gcccacggtg atgacgcgac ggccagtccg cttgcgtccg gtgccgacgg
      901 gcaggcccgg tggcggcacg cgcacgtcga tcagccgcgc ccgccctcgg gccgcggcgt
      961 cggcgagccc cggcaagctt gcgaggcgga tgtgcagtcc tgcgacgatg tcgagcccgg
     1021 ccgcagcggc ctcgcacagc gtcgtcaccc aggccgccgg gatgccgccc ccgaccgccg
     1081 ctgaacccac gatcaggctg cgtgcacccg cgctcgccgc ctgcgcaggc gtgaggtccg
     1141 gcacccccgc gtccacgcta cagcccggca acctgacctg cccgatgcag tcgtcgcgac
     1201 gccattgcgc gagcccgagc ccggtcttgg cgtaggtgtc gtcggtgatg tcgccgagga
     1261 agacgacgaa cggtgagtcg aaccggatca cgagttctgg cacggtcggt ccttcaggtg
     1321 gcactgcatt gcggcaagag gggctcgagc gacagcctcg cagcatagcc gagttgcgat
     1381 tacgtcggcc cgggcctcca ggtgaattcc cgctatgatc cgttgccgac agccaccgcg
     1441 ggacgacacg gatgagccgg gattccgacg ccgcgttcat gggacacccg aagggcctgt
     1501 tgtacctcgc cttcacggag gcgtgggagc ggttctcgta ctacggcatg acggcgctgc
     1561 tggtcctgta catggtgaac cagctgctgc tgccgggaca cgtcgagcac gtcgccgggt
     1621 tcgcggcgtt ccgcagcgcg atcgaatccg tgttcgggcc gctgtccacc caggcgctcg
     1681 cctcccaggt cttcgggctc tacagcggct tcgtctactt cacgccgatg ctgggcggcc
     1741 tcgtcgccga ccgctggatc ggacagcgcg cggccgtcgt ggtcggcgca ctcgcgatga
     1801 gcgccggcca cctcgcgatg gcgttcgatc gctcgttcct gctcgcgctc gcgctgctcg
     1861 tcgtgggctc gggcctgctg aagggcaaca tctcggcgca ggtcggggcg ctgtatccgg
     1921 tgtcggagga ggcgcggcgg acgcgcggct tcgcgatctt cagcaccggc atcaatttcg
     1981 gcgcagtcgc cgggccgctc gtgtgcggcg tgctggcgca ggccttcggc tggcactacg
     2041 gcttcgggct ggccgcggtg ttcatgctcg gggggctcgc cacgtacctg cgcggctatc
     2101 gtcacctgcc ggcacgcgtg ggacgccgcc agcacagcga gggtcgcctg actgcgaacg
     2161 actggcgcgt gatcgccgca ctcgtcgcgg tcatggtcat caccgtgttc cagtcgatcg
     2221 cgtattacca gctcgcgaac gtgctgccgg tgtggatcca ggaccacgtg gcgctcgacg
     2281 tcggcagctt caccgtgccg atcccgtggt accagtcgat cgacccgctt gcgagcatcg
     2341 tcttcgtgcc ggtgctgttc tggatctgga agcgacaggc ggccggccgc ggcgagcccg
     2401 gcgacctcgg caagatcgcg accggcgcgt gggtctgcgt cgcgagcaac ctcgtcctgg
     2461 tcggagcgat cgttgcggca cagggccggc tggtacatcc ggtctggccg ttcctctact
     2521 gcgtgggcca gggcatcggc ttcctctact actggccgac cctgctggcg ctcgtgtcgc
     2581 gcgcggcacc ggcccgcgtc aacgcgacca tgatgggctt cgcgttcatg agcctgttcg
     2641 ttgccaacac gctgattggc tgggtgggaa ccttctacga gcgactcggc ccgctgcgtt
     2701 tctggatgct gcacgcggcg atcgcggcga cgggcggcgc gctggcgctc gcgctcggtg
     2761 gcgcaatcaa gcgggtgatc gacacgggcg ccgacctgcc gctcaggccg tcggcgatga
     2821 cgctcgaggt ggagcgctag cgagcggctg aatagccgtg tgtgagagca acggattccg
     2881 agtccggaac gtggggtgga cggatttcgc cgcggcggga cgtttcagct gaaacgtcaa
     2941 ctttttgtga acccagtggc cgcggcattt cggcactgcc cctgcagtcg caaccgttgt
     3001 cgctgcacca gggacaagtc ggcgagcgcc gcagtgacct cgagtcgatg ccgggcccgg
     3061 cgatcatcct gccggcagtc tcagaacctg aggttccaca ccagttcgcc gccgcctcgc
     3121 gccggatcga acgtcacgct gaggcgctgc gagagcccgt cgcccaggaa cgcgtcgtcg
     3181 aacatccggc cgaagaagtt gccgatgctc atgccgacga ggacgtcgga cgggtaatgc
     3241 gcgccggcct cgacgcgcgc ccagccggtg gcgatggtga gcgcgtccgt gccgatgacg
     3301 agggcggtgc gcagcgagtc gtccatcggc gtgacctcga ggttgtcggc ggccagggtg
     3361 tcggcaaccg cggcgaagga cgtatgaccg gacgggaagc tgtgcgagcc accgttgggt
     3421 cgcgtgcgat tcaccgtgac cttgagacct tcggtcacga ccgactcggc agcaattgcg
     3481 ccgaggccga ccaggctgcc gctcagcttc gcatcccacc actgcgggcc cgcggcgccg
     3541 cccggagtcg caaatacgct cgcgacgtag gccgcgcccg ccgagaactc caggacgttg
     3601 ctcgcattgc tggcgttgct ggtgctgccg aagaccgggg tattgtcgat ggcccacgtg
     3661 gagacgcgcg tgtcccagtg atcgatctgc atgacggccg cgccggcgag cggggcccag
     3721 acccacggat tctttgccgc ctccagcgcg gcgtcgccga cgcggtccca accgactcga
     3781 aagcttgcgt tctcgcccca gcggctgccg tccggcagcg tcgagcaccc gctcaggagc
     3841 aaggcgacgg cacacatccg cagagtcatt gcagcctccg tcccgagccg gggaatcgag
     3901 cctggtggac aggcgtgcag tgtggacggt gatcgcccgg tagcgtccgc tcctggatca
     3961 cgattggcaa ttcgcgtgat gcaaaaaggc ccagtgattc gcctcgggac agcccgagaa
     4021 tcctgcgggt aaatatttct tgttcacccg cgattggttt tccgcagacc cgacgtccaa
     4081 cgtctggggg ccggggaagt tccctcgggt cacggcgcat gtgcgcgcag aaattcgatg
     4141 gaaatttctt gaacttcccg tcgcaccgcg agtccatttt gaaccatggc cagcgacaac
     4201 cggatcacgg cactcacgct caagtcacgc cgcgccgcgg tggcgacacc gctccctgac
     4261 gccgatcacc tgaccgcgtg ggccgcgcac cgcgaagccc tcgacgtcgg tcgcatcatc
     4321 gagtcacatc acgcggcgct cg
//
LOCUS       DAOMFD010000165         4331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8912_length_4331_cov_36.020814, whole genome shotgun sequence.
ACCESSION   DAOMFD010000165 DAOMFD010000000
VERSION     DAOMFD010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4331
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8912_length_4331_cov_36.020814"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="PL152_08775"
     CDS             complement(<1..688)
                     /locus_tag="PL152_08775"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004365 - glyceraldehyde-3-phosphate
                     dehydrogenase (NAD+) (phosphorylating) activity [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQR49418.1"
                     /translation="MTIKVGINGFGRIGRMVFRAAVRDFKDIEIVGINDLLEPDYLAY
                     MLKYDSVHGRFKGDVAVEGSTLVVNGRRIRLTAVKDPAELKWKDVGADVVVESTGLFL
                     TKETAEKHLAAGAKKVIMSAPSKDDTPMFVYGVNDRTYAGQAIISNASCTTNCLAPVA
                     KVLNDTWGIKRGLMTTVHAATATQKTVDGPSNKDWRGGRGILENIIPSSTGAAKAVGK
                     VIPELNKKLTG"
     gene            complement(716..2719)
                     /gene="tkt"
                     /locus_tag="PL152_08780"
     CDS             complement(716..2719)
                     /gene="tkt"
                     /locus_tag="PL152_08780"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HQR49419.1"
                     /translation="MPTRRQLANAIRALSMDAVQKANSGHPGMPMGMADIAQVLWGDH
                     LKHNPANPTWADRDRFVLSNGHGSMLLYSLLYLTGYPLGIEDLKNFRQFGSHTAGHPE
                     VDPHLGIETTTGPLGQGIANAVGMALAEKILAATFNRPGHEVVDHRTWVFLGDGCLME
                     GVSHEACSLAGTLGLGKLTAFYDDNGISIDGEVHGWFTDDTPKRFEAYGWQVIRDVDG
                     HDAGAIERAIAAATAQTTRPTLICCKTIIGWGAPNKQGTEATHGAALGEAEVAAARER
                     LGWPYEPFVIPDPIKHAWDARERGAKAERDWQAKLASYRHAHPGLAAEFERRLAGRLP
                     EGWRKALDDFMRAAQEKPAAVATRSSSQQVLNVLGAVIPELLGGSADLTGSNNTNHKG
                     SKTVTHADIAGNYLHYGVREFGMTAIMNGVSLHGGFVPYGGTFLVFSDYARNAVRMAA
                     LMKQGVILVYTHDSIGLGEDGPTHQPVEHVASLRLIPNMRVWRPCDTVETAVAWADAV
                     DRRAAPTSLVLSRQALPAMPREASQVADIAKGGYVLADCAGKPDCVLIATGSEIALAM
                     DAAKALAEGGRKVRVVSMPCTDAYDAQPQPYRDSVLPPGVPRVAIEAGVRDGWWRYVS
                     CGGAVIGMDSFGASAPAKQLFEHYGFTRENVIRTVQGLLQSRS"
     gene            2946..4172
                     /gene="metK"
                     /locus_tag="PL152_08785"
     CDS             2946..4172
                     /gene="metK"
                     /locus_tag="PL152_08785"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HQR49420.1"
                     /translation="MSNSYLFTSESVSEGHPDKVADQISDAVLDAMLAQDKRARVACE
                     TLVKTGVAIVAGEVTTEAWVDLEELVRGVITDIGYTSSNVGFDGRTCGVLNIIGKQSP
                     DIAIGVDRAKPEEQGAGDQGLMFGYASNETESLMPAPICYAHRLVERQAEVRKSHLLP
                     WLRPDAKSQVSCRYENGKVVGLDAVVLSTQHDPDVKLEDLREAVMELIIKHVLPREWL
                     GKGTKFHINPTGNFVIGGPVGDCGLTGRKIIVDSYGGMARHGGGAFSGKDPSKVDRSA
                     AYAARYVAKNIVAARLADRCEIQISYAIGVAEPTSITVETFGTGKVEPARLEKLVRAH
                     FDLRPYGIIKMLDLVHPMYRPTAAYGHFGREPVHMTYKWKEYIGGKKVEKSEAFTAFT
                     WEKTDKAEALRKDAGL"
     regulatory      4247..4323
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01057"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="S-adenosyl-L-homocysteine riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosyl-L-homocysteine"
                     /db_xref="RFAM:RF01057"
BASE COUNT          698 a         1471 c         1430 g          732 t
ORIGIN      
        1 tgccggtgag cttcttgttg agctcgggga tgaccttgcc caccgccttc gcggcgccgg
       61 tcgacgacgg gatgatgttc tcgaggatgc cgcggccgcc gcgccagtcc ttgttcgacg
      121 ggccgtcgac ggtcttctgc gtcgcggtcg ccgcgtgcac ggtcgtcatc aggccgcgct
      181 tgatgcccca ggtgtcgttc agcaccttgg ccaccggcgc gaggcagttc gtggtgcagg
      241 acgcattgga gatgatcgcc tgcccggcgt aggtccggtc gttcactccg tagacgaaca
      301 tcggcgtgtc gtccttggac ggcgccgaca tgatgacctt cttcgcgccg gccgcgaggt
      361 gcttctccgc ggtctccttg gtgaggaaca gtcccgtcga ctcgaccacc acgtccgcgc
      421 cgacgtcctt ccacttgagc tcggccgggt ccttgaccgc cgtcaggcgg atcctgcggc
      481 cgttgaccac cagcgtgctt ccctcgacgg cgacgtcgcc cttgaagcga ccgtgcaccg
      541 agtcgtactt cagcatgtag gccaggtagt ccggctcgag gagatcgttg atgccgacga
      601 tctcgatgtc cttgaagtcg cgcaccgccg cgcggaacac catgcgcccg atccgaccga
      661 atccattgat gccaaccttg atcgtcattg ctcgacctcc cgggaagaaa ttccgtcagc
      721 tgcgggactg cagcagtccc tgtaccgtcc tgatcacgtt ctcgcgcgtg aagccgtagt
      781 gctcgaacag ctgcttcgcg ggcgccgagg caccgaagct gtccatgccg atcacggcac
      841 cgccgcagga tacgtatcgc caccagccgt cgcgcacgcc ggcttcgatc gcgacgcgtg
      901 gcacgcccgg cggcagcacg ctgtcacgat acggctgcgg ctgcgcgtcg taggcgtcgg
      961 tgcacggcat ggagacaacg cgcaccttgc gtccgccttc cgccagggcc ttcgctgcat
     1021 ccatcgccag cgcgatctcc gaacccgtcg cgatcagcac gcaatccggc ttgcccgcgc
     1081 agtccgccag gacgtatccg cccttggcga tgtcggcgac ctgactcgcc tcgcgcggca
     1141 tggctggaag cgcctgccgg ctcaaaacaa ggctcgtcgg cgcggcgcgg cggtcgaccg
     1201 cgtcggccca cgccaccgcc gtttccaccg tgtcgcaggg ccgccacacg cgcatattcg
     1261 ggatcagacg gagactcgcg acgtgttcca cgggctggtg cgtgggcccg tcctcgccca
     1321 gcccgatcga gtcgtgcgtg tagaccagga tcacgccctg cttcatcagg gccgccatcc
     1381 gcacggcgtt gcgcgcgtag tccgagaaga cgaggaacgt gccgccgtag ggcacgaagc
     1441 caccgtgcag cgacacgccg ttcatgatcg cggtcatgcc gaattcgcgc accccgtagt
     1501 gcaggtagtt gccggcgatg tccgcgtgcg tgacggtctt cgaccccttg tgattcgtgt
     1561 tattggagcc ggtgaggtca gccgacccac cgagcagttc aggaatgacg gcgccgagca
     1621 cgttcagcac ctgttgcgat gaagaccggg tcgccacggc cgcaggcttc tcctgcgcgg
     1681 cgcgcatgaa atcgtccagt gccttgcgcc agccctccgg caatcgcccg gcgagcctgc
     1741 gctcgaactc ggcggccagc cccgggtgcg catggcgata ggaagcgagc ttggcctgcc
     1801 agtcgcgctc ggcctttgcg cctcgctcgc gcgcgtccca ggcgtgcttg atcgggtcgg
     1861 gaatcacgaa cggctcgtac ggccagccga ggcgctcgcg cgccgcggcg acctcggctt
     1921 cgcccagcgc cgcgccgtgc gtggcctcgg tgccctgctt gttcggagcg ccccagccga
     1981 tgatcgtctt gcagcagatg agcgtcggcc gcgtggtctg cgccgtggcg gcggcgatgg
     2041 cgcgctcgat cgcgccagcg tcgtggccgt ccacgtcgcg gatcacctgc cagccgtagg
     2101 cctcgaaacg cttcggggtg tcgtccgtga accagccgtg cacctcgccg tcgatcgaga
     2161 tgccgttgtc gtcgtagaag gccgtgagct tcccgagtcc gagcgtcccc gccagcgagc
     2221 acgcctcgtg ggacacgcct tccatcaggc agccgtcgcc gaggaagacc caggtgcggt
     2281 ggtcgacgac ctcgtgcccg ggccggttga acgtcgccgc caggatcttc tccgccagcg
     2341 ccatgcccac ggcgttggcg atgccctggc ccagcggccc cgtggtcgtc tcgatgccga
     2401 ggtggggatc gacttccggg tgcccggccg tgtgggagcc gaactgccgg aaattcttca
     2461 ggtcctcgat gccgagcggg tagccggtca ggtatagcag cgagtacagc agcatcgagc
     2521 cgtggccgtt cgacagcacg aagcgatcac gatcggccca ggtcgggttg gccgggttgt
     2581 gcttgaggtg gtcgccccac agcacctgtg cgatgtccgc catgcccatc ggcattcccg
     2641 ggtgaccgga attggctttc tggaccgcat ccatgctcaa ggcacggatc gcgttggcga
     2701 gctgcctgcg tgtcggcata gtcggtgaac cctcgaggta gtcacgtatt ttcgcgcctc
     2761 gggtgccggg tttgcaaagt cgtggatgat cacgatggat gccggcgcgg gcgggctgcg
     2821 atcccctggg ttccacgccc ccgcgacgaa gcgtgccggc tatatcgata gtgccagccg
     2881 gtttgccaag cgggcgcccg gcggcagata cttcgcgccc tgttggtgcg accggaatgc
     2941 cattaatgtc aaacagttac ctgttcacgt cggagtccgt ctcggaaggc catcctgaca
     3001 aggtcgccga ccagatctcc gacgccgtgc tcgatgcgat gctcgcgcag gacaagcggg
     3061 cccgggtcgc gtgcgagacc ctggtcaaga ccggtgtcgc aatcgtcgcc ggcgaggtga
     3121 ccaccgaggc ctgggtcgac ctcgaggaac tggtgcgcgg cgtcatcacc gacatcggct
     3181 acacctcgtc gaacgtcggg ttcgacggcc gcacctgcgg cgtgctcaac atcatcggca
     3241 agcagtcgcc cgacatcgcg atcggcgtcg atcgcgcgaa gcccgaggag caaggggccg
     3301 gcgaccaggg cctgatgttc ggctacgcgt cgaacgagac cgagtcgctg atgccggcac
     3361 ccatctgcta tgcacatcgc ctggtcgagc gccaggccga ggtgcgcaag agccacctcc
     3421 tgccgtggct gcggccggac gcgaaatcgc aggtgagctg ccgctacgag aacggcaagg
     3481 tggtcgggct ggacgccgtc gtgctgtcga cccagcacga cccggacgtg aagctcgagg
     3541 acctgcgcga agcggtcatg gagctcatca tcaagcacgt gctgccgcgc gagtggctcg
     3601 gcaagggcac caagttccac atcaacccca cgggcaattt cgtgatcggc gggccggtcg
     3661 gggactgcgg gctcaccggc cgcaagatca tcgtggacag ctacggcggc atggcacgcc
     3721 acggcggcgg cgcgttctcg ggcaaggacc cgtccaaagt cgaccgctcc gctgcgtacg
     3781 cagcacggta cgtcgcgaag aacatcgtcg cggccagact cgcggatcgc tgcgaaatcc
     3841 agatctccta cgcgatcggc gtcgccgagc cgacctcgat caccgtcgag accttcggca
     3901 cgggcaaggt cgagccggcg cgcctggaga aactggtgcg cgcgcatttc gacctgcggc
     3961 cctacggcat catcaagatg ctcgacctcg tgcacccgat gtaccggccg acggccgcct
     4021 acggccactt cggccgggag ccggtccaca tgacctacaa gtggaaggaa tacatcggcg
     4081 gcaagaaggt cgagaagagc gaggcgttca cggccttcac ctgggagaag accgacaagg
     4141 ccgaggcgct gcggaaggac gccgggctgt aggaaaatgg ggacattctc ctatttcccg
     4201 aggcgggaaa taggagaatg tccccgcttt ccgttacaat tcgccatcgc ctgaggagcg
     4261 ctgcaacggg cccaagcccg ccaggctcag gcacctgggt tatcccaacc gcgctcgctg
     4321 acctccgtgc a
//
LOCUS       DAOMFD010000166         4317 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8967_length_4317_cov_35.027687, whole genome shotgun sequence.
ACCESSION   DAOMFD010000166 DAOMFD010000000
VERSION     DAOMFD010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4317)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4317
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8967_length_4317_cov_35.027687"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /gene="argS"
                     /locus_tag="PL152_08790"
     CDS             complement(<1..422)
                     /gene="argS"
                     /locus_tag="PL152_08790"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF015450.2"
                     /note="catalyzes a two-step reaction, first charging an
                     arginine molecule by linking its carboxyl group to the
                     alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; class-I aminoacyl-tRNA
                     synthetase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HQR49421.1"
                     /translation="MKYLIETLVRDALAALPADLKGAGATATSPVIERTRDTAHGDFA
                     TNAAMQLAKSAKRKPRDVAQAIVDALPASPLVARVEIAGPGFINFHLAPAAYHQELVR
                     VLELGTAYGRSQLGAGQRVIVEFVSANPTGPLHVGHGR"
     gene            complement(497..>1717)
                     /locus_tag="PL152_08795"
     CDS             complement(497..>1717)
                     /locus_tag="PL152_08795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49422.1"
                     /translation="TSSFSFRGRQLGIAAIAGELGVAHVLEGSVRRSGDRIRITAQLI
                     RASDSSHVWSQAFDRNLSDIFAVQDEIAAAVTRELEIKLLGRPAPKARQTDPEAYTHF
                     LRGRHFFELASKSGYEQAIVELEAALAIDPDFGPAHGLLGAVYWGMANNQLIDYAEGA
                     RKARAESEKALALDPDLAEPLSLLGYFDVIEARDVDFGLRRVERALQLEPNNQRILTR
                     AANLAIRRGRLDDALRYSRLAIAADPLGANPHAICGNVCYYAGRLEEAEAMRRKVLAL
                     SPGWLSGHFNLAKVLLARGDAPGAHAELQLEQSDFWRFTGLAIVQHALGQQAESDEAL
                     RNLMQMDLGGADYQLVQVHASRGEIDTAFEWLDKAIETHDSALVFIRFDPLIENLRAD
                     PRWPAILARLGQAA"
     assembly_gap    1718..1817
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1818..1999)
                     /locus_tag="PL152_08800"
     CDS             complement(<1818..1999)
                     /locus_tag="PL152_08800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49423.1"
                     /translation="MRFLAELKRRRVIRTALVYLASSWLLLQVAELLLGMLDVPAWGL
                     KLVFVLLAIGFPMALIL"
     gene            complement(2085..4286)
                     /locus_tag="PL152_08805"
     CDS             complement(2085..4286)
                     /locus_tag="PL152_08805"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HQR49424.1"
                     /translation="MSPVLAVAIDTPLRRVFDYRAPPHCTQEMLVPGVRLWVPFGKRR
                     VVGVLVELRDHSELPDAKLKAAVAIIDDEPAIDAPLLDLLRWAADYYRHPPGEVIAAA
                     LPAPLRTGDSMVETTERWSLSAAVRTGSLPALSGRANRLRDMAQFLADRDSADAAGLA
                     TLSVRWRDHARELEKRGWITRLRTAEPALPRSETHAGPAPEPTAEQRAAIDAIAAARG
                     RNASFLLHGVTGSGKTEVYLRAIEAVVARGEQALVLVPEIALTPQLVARFAARFAAPL
                     AVLHSSLTDQERLRAWRAARSGAAPVVIGTRSAVFAPLRRPGLIVVDEEHDPSYKQQE
                     GFRYSARDLALARAQRLAIPVVLGSATPSLESLERANSGKTTLLPLPNRTAGATQPRL
                     QVVDLRRHADTQGIATPTMLAIQRHLDAGGQVLLYLNRRGYAPTLFCPSCGWIAPCPR
                     CDARMTVHLRDRRLHCHHCGTEQQLPETCASCGEPVKPVGQGTERIEETLAQLFPGTP
                     LARIDRDAVRRRGALEAALDRVTRGEVRLLVGTQMLAKGHHFPDVSLVVVLNADQGLF
                     GTDFRASERLAQTIIQVAGRAGRAERPGEVLIQTQFPEHPLLAQLVGGGYDAFAVAAL
                     EERRQARWPPFARIAVLRAEAARREAPLEFLDRARSIAISLAGRDVEVLGPASAPMER
                     RAGHYRAQLLLHAASHAPLQRLFARWVPALEILPEARKARWSLDVDPLELF"
BASE COUNT          618 a         1425 c         1543 g          631 t
ORIGIN      
        1 tgccggccat ggccgacgtg cagcggtccc gtcgggttgg cggatacgaa ttccacgatg
       61 acgcgctggc cggccccgag ctggctgcga ccgtaggccg tgccgagctc gaggacgcgg
      121 acgagttcct ggtggtacgc ggccggcgcg aggtggaaat taatgaatcc ggggccggcg
      181 atctcgaccc tggcgacgag cgggctcgcc ggcagtgcgt ccacgatggc ctgggccacg
      241 tcccgcggct tgcgcttcgc cgactttgca agctgcatcg ctgcgttggt cgcgaagtcg
      301 ccgtgggcgg tgtcgcgcgt gcgctcgatc accggtgagg tcgcggtcgc gccggccccc
      361 ttgaggtcgg caggcagcgc cgcaagcgcg tcccgcacca gagtttcgat caggtatttc
      421 aaggtgttgc ccgggcaagc cggccggcga gggcggtcag tctagggaat tcccggcggg
      481 gatgcgagtc caaggctcag gccgcttgtc ccagccgggc gagaatcgcg ggccagcgcg
      541 gatccgcgcg caggttttcg atcagcgggt cgaagcgaat gaataccagc gcgctgtcgt
      601 gcgtctcgat ggccttgtcc agccattcga acgcggtgtc gatttcgccg cgggacgcgt
      661 ggacctggac cagctgatag tcggcgccgc cgaggtccat ctgcatgagg ttgcgtagcg
      721 cctcgtcgga ttccgcctgc tggcccaaag cgtgctggac gatcgcgagt cccgtaaacc
      781 gccagaagtc gctctgttcg agctgcagct ccgcgtgcgc ccccggtgcg tcaccgcgtg
      841 ccagcaggac cttcgcgagg ttgaagtggc cgctcagcca tccgggactc agcgccagca
      901 ccttccggcg catggcctcg gcttcctcca ggcgtccggc gtagtagcac acgttgccgc
      961 agatcgcgtg cgggttcgcg cccaggggat cggccgcgat ggccagccgg ctgtagcgaa
     1021 gtgcgtcgtc cagtcggccg cggcggatgg cgaggttcgc ggcccgcgtc aggatgcgct
     1081 ggttgttcgg ttcgagctgc agcgcccgct ccacgcgccg cagcccgaag tcgacgtcac
     1141 gcgcttcgat cacgtcgaag tatccgagca gcgacagggg ttcggccagg tccggatcca
     1201 gggccagcgc cttctccgac tcggctcgcg ccttgcgcgc cccttctgcg tagtcgatca
     1261 gctggttgtt cgccatgccc cagtagacgg cgccgagcag tccatgcgct ggcccgaagt
     1321 ccgggtcgat cgccagtgcg gcctcgagtt cgacgatggc ctgctcgtag ccggacttcg
     1381 acgcgagttc gaagaagtgc cggccgcgca ggaaatgcgt gtatgcctcg gggtcggtct
     1441 gccgcgcctt cggtgccggg cggccgagca gcttgatctc cagctcgcgc gtgaccgccg
     1501 cggcgatctc gtcctggacc gcgaagatgt cgctcaggtt gcgatcgaag gcctgcgacc
     1561 agacgtgcga gctgtcactg gcgcggatca gctgtgccgt gatgcggatg cgatcgccgg
     1621 accggcgcac gctgccctcg agcacgtgcg cgacgccgag ttccccggcg atcgccgcga
     1681 tgcccagctg ccgccccctg aacgagaacg acgaggtnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnnagg atcagcgcca tcgggaagcc gatcgcgagc agcacgaaga
     1861 cgagcttcag tccccaggcc ggcacgtcga gcatgccgag cagcagttca gcgacctgca
     1921 ggagcagcca ggacgacgcc aggtagacca gcgcggtccg gatgactctg cgacgcttga
     1981 gctccgcgag aaatcgcatt caggtcctgc cccccgatcc ccggtcggac gacccgggtc
     2041 tcgctggcac gagagtacgc ggacccacgg gcgcgtggta gcgcctagaa cagttccagc
     2101 gggtccacgt ccagcgacca gcgcgccttg cgcgcctcgg gcaggatctc gagcgccggg
     2161 acccagcggg cgaagagccg ctgcagcgga gcgtgcgacg cggcgtgcag caggagttgc
     2221 gcgcggtagt gcccggcccg ccgctccatc ggtgccgacg caggcccgag gacctcgacg
     2281 tcccggccgg cgagcgaaat ggcgatgctc cgtgctcgat ccaggaactc gagcggcgcc
     2341 tcgcggcggg cggcttccgc ccgcagtacc gcgatcctgg cgaacggcgg ccagcgcgcc
     2401 tgcctgcgtt cctcgagcgc ggccacggcg aatgcgtcgt agccgccgcc gacgagttgc
     2461 gcgagcaacg ggtgttccgg gaattgtgtc tggatcagca cctcgccggg acgctccgcg
     2521 cggccggcgc gcccagcgac ctggatgatg gtctgcgcga gccgctcgct ggcgcggaag
     2581 tccgtgccga acaggccctg gtcggcgttg agcacgacga cgaggctcac gtcggggaaa
     2641 tggtgtccct tcgccagcat ctgcgtgccg accagcaacc ggacctcgcc gcgcgtgacg
     2701 cgatcgagcg ccgcctccag cgcgccacgg cgacgcaccg cgtcgcgatc gatgcgggcg
     2761 agcggggtgc cgggaaacag ctgcgcgagg gtctcttcga tgcgctcggt gccttgcccc
     2821 acgggcttca ccggctcgcc gcagctcgcg catgtctcgg gcagctgctg ctcggtgccg
     2881 cagtgatggc agtgcaggcg ccgatcgcgc aggtgcacgg tcatgcgcgc gtcgcagcgc
     2941 gggcagggag cgatccagcc gcaggacggg cagaacagcg ttggcgcgta gccgcggcga
     3001 ttgaggtaca gcagcacctg cccgcccgcg tcgaggtgcc gctggatcgc gagcatggtc
     3061 ggagtggcga tgccctgcgt gtcggcgtgc cggcgcaggt cgacgacctg caagcgcggt
     3121 tgcgtggcgc ccgccgtgcg gttcggcagg ggcagcagcg tggtcttccc ggagttggcg
     3181 cgctcgagac tctcgagcga aggggtcgcc gagccgagga cgaccggaat ggccaggcgt
     3241 tgcgctcgag cgagagcgag gtcgcgcgcc gagtaacgga atccttcctg ctgcttgtag
     3301 gacgggtcgt gctcctcgtc cacgacgatg agtcccggtc gcctgagggg tgcgaaaacg
     3361 gcggatcgcg taccgatcac caccggggcg gcaccgctgc gggccgctcg ccaggcacga
     3421 aggcgttcct ggtcggtgag cgaggagtgc agcaccgcga gtggcgcggc gaagcgtgcc
     3481 gcgaagcggg ccacgagctg cggcgtcagg gcgatctccg gcaccagcac gagggcctgt
     3541 tcgccacgag ccacgaccgc ttcgatcgcg cgcaggtaca cctcggtctt gccgctgccc
     3601 gtgacgccgt gcagcaggaa cgaagcgttg cgcccgcggg ccgcggcgat ggcatcgatt
     3661 gcggcgcgct gctccgcggt cggctccggg gccggaccgg cgtgcgtctc gctgcggggc
     3721 agggcgggct cggccgtccg cagtcgagtg atccagccgc gcttctcgag ttcgcgggcg
     3781 tggtcgcgcc accggacgga aagtgtcgcg aggccggcag cgtcggcact gtcgcgatcc
     3841 gcgaggaact gcgccatgtc ccgcagccgg ttcgcacgac cggacagggc aggcaggctg
     3901 cccgtgcgca ccgccgcgct gagcgaccat cgttcggtgg tctcgaccat cgagtcgccg
     3961 gtgcgcagcg gcgccggcaa cgccgccgcg atcacttcgc cgggcggatg acggtagtag
     4021 tcggcggccc agcgcagcag gtccaggagc ggcgcgtcga tcgccggctc atcgtcgatg
     4081 atcgcgacgg ctgccttgag tttcgcgtcc ggcagctccg agtgatcgcg cagctcgacc
     4141 agcactccga ccacgcgacg cttcccgaac gggacccaca accgcacccc gggcacgagc
     4201 atttcctggg tgcaatgcgg cggtgcgcgg tagtcgaaga cgcggcgcag cggcgtgtcg
     4261 atggcgacgg cgaggaccgg gctcacggtc gagcaggctt atgtgaagtt gtccaca
//
LOCUS       DAOMFD010000167         4313 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_8983_length_4313_cov_31.250822, whole genome shotgun sequence.
ACCESSION   DAOMFD010000167 DAOMFD010000000
VERSION     DAOMFD010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4313)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4313
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8983_length_4313_cov_31.250822"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(78..1487)
                     /locus_tag="PL152_08810"
     CDS             complement(78..1487)
                     /locus_tag="PL152_08810"
                     /inference="COORDINATES: protein motif:HMM:NF006596.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQR49425.1"
                     /translation="MRLPIVATCAALCLLTATPAPADALRPDQVAYRELFKELVETNT
                     TLSSGNCTEAAEKMAARLRKAGMPESDIHLFTVPDHPKEGGLVAILPGTDATERPMLL
                     LAHIDVVEAKRADWTRDPFTLYEEDGYFYARGVADDKAMASIWVDTLVRFLQDGYKPR
                     RTVKMALTCGEETSGAFNGAEYLSTQQRDLIDAAFALNEGAWGTLDEQGRRVMLTVQA
                     GEKASQNYSLEVRNPGGHSSRPVKDNAIYRLAAGLIHLSAFEFPVRLNDATRVYFTRM
                     ADIKGGATGAAMRALVKNPSDAQAIATITQDPTWNAMLRTTCVATMMDAGHATNALPQ
                     RARANVNCRIYPDQSFEDVRQALERVVADPEIKVTTLETRGPMAKAPPLTPEIMGPIE
                     QVAAKVFPGVPVLPLFQAGATDGQFLGAAGIPTYGIGVLFFDPDLGGIHGLNERIRVQ
                     SLYDGRDFLYDLVRVYSAQ"
     assembly_gap    1717..1816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1817..3951)
                     /locus_tag="PL152_08815"
     CDS             complement(<1817..3951)
                     /locus_tag="PL152_08815"
                     /inference="COORDINATES: protein motif:HMM:NF024763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HQR49426.1"
                     /translation="MDSQLRSQVVFHLTGRLQESAAAAVPGLRPALLARYRDLDALRH
                     DFPVVLCDAGEDCIQPLSDVVDRALRKVAPAGATGEALRRRVLKVEKQIRRRVAKGAH
                     GKLTAVWGEATQVIAGADAAFARDGARAREAFDVDGEVADCDASLPGRLVRHAWSAVQ
                     GQKAQAARARIEQLSIRLGEILRADYMRSEQALLQPALKSSFGAAHHGLFDFAAMSRM
                     LQRVGPAGGLSDRRRARIERALAVLRSQRFFAPAVGADARFHQFAFEDAGAALAAYRE
                     RLPELAELVAALQVAELEVEGSYLEELHDPIFAALDGDAVTAHDLQFFPDYLVCLGRP
                     GDSGPARLAEALSSGVPIKVLVQVEDLVEDFAPGRGHPAFGLRSAQLASAALSFDDVF
                     VLQSAASNLVQLRSRLLHGLRHAGPALFSVYAGPADGALPTYLSSAAAMQSRAFPAFS
                     YDPAAGPDLASRFSLENNPQVERDWPVETLAYADQDLQSVTEQVAFTFLDFALCDARC
                     GSHFAAVPRAAWGDTMIPAHEWLEHPPQDAASGVPYVLAVDDGDLLCRLVVDDRLVRA
                     AVRCREGWHRLQELGGVHDSRVERLLAKERQAWEEQHQQTLQGAPATQGPALPEVTGK
                     DAGAAIAPVAAEEAAEVARNPDEPYIETIRCSTCNECTQVNPRMFAYNDNKQAYIADL
                     KAGTYAQLVEAAESCQLSIIHPGKPWNPD"
     gene            complement(3954..>4313)
                     /locus_tag="PL152_08820"
     CDS             complement(3954..>4313)
                     /locus_tag="PL152_08820"
                     /inference="COORDINATES: protein motif:HMM:NF023785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49427.1"
                     /translation="HRGFSFVRILQRCPEFVGNMFEPWVHDPQRMLMLTHADGIGLSP
                     ALQKIYHNVEQHDPSDMNRAREVASIDDPIPVGILYRNPEVPCYEDLRQAGALRTAER
                     IRGTLDAEFDKFTVWPQ"
BASE COUNT          652 a         1454 c         1422 g          685 t
ORIGIN      
        1 gcggcgcggc ccgcgagtgc gatcgctacc tgatgggcaa gaccgacctg ccataggaat
       61 aggggacatt cccctattta ttgcgcggag tagacgcgca ctagatcgta gaggaagtcg
      121 cgcccgtcgt agagcgactg cacgcgaatg cgctcgttca ggccgtggat cccgccgagg
      181 tccgggtcga agaacagcac gccgatgccg taggtcggga tgccagccgc cccgaggaac
      241 tgcccgtccg tcgcgcctgc ctggaacagc ggcagcacgg gcacgcccgg gaataccttc
      301 gcggccacct gctcgatcgg ccccatgatc tcgggcgtga gcggcggggc cttggccatc
      361 ggcccgcggg tctcgagggt cgtgaccttg atctccgggt cggccaccac gcgttcgagc
      421 gcctggcgga cgtcctcgaa cgactggtcc gggtagatcc ggcagttgac gttggcgcgc
      481 gcgcgttgcg gcagcgcgtt ggtcgcgtgg ccggcgtcca tcatcgtcgc gacacacgtc
      541 gtgcgcagca tcgcgttcca ggtcgggtcc tgcgtgatgg tcgcgatggc ctgcgcgtcg
      601 gacgggttct tcaccagggc gcgcatcgcc gcgcccgtcg ccccgccctt gatgtccgcc
      661 atgcgcgtga agtacacgcg cgtcgcgtcg ttgagccgaa ccgggaattc gaatgcactg
      721 aggtggatca ggcccgcggc cagccggtag atcgcgttgt ccttgaccgg ccgcgagctg
      781 tggccgcccg ggttgcgcac ctcgagcgag tagttctggc tcgccttctc gcccgcctgc
      841 accgtcagca tgacacgacg accctgctcg tcgagcgtgc cccacgcgcc ctcgttgagc
      901 gcgaaggcgg cgtcaatcag gtcgcgttgc tgggtcgaga ggtattccgc tccgttgaag
      961 gcgccgctcg tctcctcgcc gcaggtgagc gccatcttga cggtacggcg cggcttgtag
     1021 ccgtcctgca ggaaccgcac gagcgtgtcc acccagatcg aggccatggc cttgtcgtcg
     1081 gcgacgccgc gcgcgtagaa gtaaccgtcc tcctcgtaga gcgtgaaggg gtcgcgcgtc
     1141 cagtccgcgc gcttcgcctc cacgacgtcg atgtgggcga gcagcagcat cggtcgctcg
     1201 gtcgcgtccg tgcccggcag gatcgcgacg agcccgccct ccttcggatg atccggcacg
     1261 gtgaacaggt gaatgtcgct ctcgggcatg cctgccttgc gcagcctggc cgccatcttt
     1321 tccgcagcct cggtgcagtt gcccgaggac agcgtggtgt tggtctcgac gagctccttg
     1381 aagagctcgc gataggcgac ctggtcgggc cgcagcgcgt cggccggcgc aggtgtcgcg
     1441 gtcagcaggc acagcgcggc acaggtcgca acgatcggca gtcgcatgct cttcacgtga
     1501 ccagccctcg ggaaagcaga caagaagggg gtcgtcatcg tatcaggcgg cacgacgacg
     1561 accggggaca ttctcctatt tcccgccgcg ggaaatagga gaatgtcccc atctcctact
     1621 ggaacggctt cgcgcgctcg acgagttccg cgagaccggg ttcgtccggg ttccacggct
     1681 tgcccgggtg gatgatcgag agctggcagc tctccgnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnntcgt ccgggttcca cggcttgccc gggtggatga tcgagagctg
     1861 gcagctctcc gcggcctcca ccagctgcgc atatgtgccc gccttgagat cggcgatgta
     1921 ggcctgcttg ttgtcgttgt aggcgaacat gcgcgggttg acctgcgtgc actcgttgca
     1981 cgtcgagcac cggatggtct cgatgtaggg ttcgtcggga ttgcgcgcca cctcggcggc
     2041 ttcctcggcg gcgactggtg cgatcgcggc acccgcgtcc ttgcccgtaa cctccggcag
     2101 tgcgggaccc tgcgtcgcgg gcgcaccctg cagcgtctgc tggtgctgct cctcccacgc
     2161 ctgccgctcc ttcgcgagca gcctttccac gcgcgaatcg tgcacgccgc ccagttcctg
     2221 caggcggtgc cagccttcgc gacagcgcac ggccgcgcgc accagcctgt catccacgac
     2281 cagtcgacac aaaaggtcgc catcgtccac cgccagcacg tacggcacgc cgctcgccgc
     2341 gtcctgcggt ggatgctcga gccattcgtg cgccgggatc atcgtgtcgc cccacgctgc
     2401 ccgcggcacg gcggcgaagt ggctgccaca gcgggcgtcg cacaacgcga agtcgaggaa
     2461 cgtgaaggcg acctgctcgg tcacggactg caggtcctgg tcggcatagg cgagcgtctc
     2521 gaccggccag tcgcgctcga cctgcggatt gttctcgagc gagaaccgcg acgcgaggtc
     2581 cggtccagcg gctggatcgt agctgaacgc tggaaacgcc cgggactgca tcgccgccgc
     2641 ggatgacagg tacgtaggca gggccccatc tgctggtccg gcatacacgc tgaagagcgc
     2701 cggtcccgca tgacgcaacc cgtgcaggag tcgcgatcgc agctgcacca gattcgacgc
     2761 ggcggactgc agcacgaaca cgtcgtcgaa gctgagcgcc gcactcgcga gctgcgcgct
     2821 ccgcagcccg aaggccgggt gaccgcggcc cggcgcaaag tcctcgacga ggtcctcgac
     2881 ctgcaccagg accttgatcg gcacgccgga cgagagcgcc tcggcgagcc gcgcgggccc
     2941 gctgtctccg ggtcgaccca ggcagaccag gtagtccggg aagaactgca ggtcatgtgc
     3001 ggtgacggcg tcgccgtcca gcgcggcgaa gatcgggtcg tgcagttcct cgaggtagct
     3061 gccctcgact tccagctcgg ccacctgcag cgccgcgacc agctctgcaa gctccggcag
     3121 gcgctcacgg taggccgcaa gcgcggcccc ggcgtcctcg aaggcgaatt gatggaatcg
     3181 cgcgtccgca cccaccgctg gtgcgaagaa ccgttgcgac cgaagcaccg ccagcgctcg
     3241 ctcgatgcgg gcacggcgcc ggtcgctcag cccgcccgcc gggccgactc gctgcagcat
     3301 gcgcgacatt gcagcgaagt cgaacaaccc gtgatgggcg gcgccgaacg aggatttcaa
     3361 cgcaggctgc agcagcgcct gctcggacct catgtagtcc gcgcgcagga tctcgccgag
     3421 cctgatcgac agctgctcga tacgcgcgcg cgcggcctgg gccttctgcc cctgcacggc
     3481 ggaccacgcg tgccggacga ggcgccctgg caacgatgcg tcgcaatcgg caacttcacc
     3541 gtcgacgtcg aacgcttcgc gcgcacgggc accatcgcgg gcaaacgccg catcggctcc
     3601 cgcgatcacc tgcgtcgcct cgccccacac agccgtcagc ttcccgtgtg cacctttcgc
     3661 gacccgacgg cggatctgct tctcgacctt gagcacgcgc cggcgcagcg cctcgccggt
     3721 cgctcccgcg ggggcgacct tgcgcagtgc gcgatcgacc acgtcggaca gcggctgaat
     3781 gcagtcctcg ccggcgtcgc agaggacgac gggaaagtcg tggcgaagtg catccaggtc
     3841 gcgataccgc gcgagcagcg cgggacgcag gccggggact gccgcggcgg cactctcctg
     3901 aaggcgaccg gtgaggtgga agacgacctg tgacctgagc tggctgtcca tggttactgc
     3961 ggccacacgg tgaacttgtc gaattccgca tcgagcgtgc cgcgaatgcg ctccgcggtt
     4021 cgcagggcgc ccgcctgcct tagatcctcg tagcacggca cttcggggtt gcggtacagg
     4081 atgccgaccg ggatcggatc atcgatcgac gcgacctccc gcgcccggtt catgtccgac
     4141 ggatcgtgct gctcgacgtt gtgatagatc ttctgcaggg ccgggctcaa cccgatgcca
     4201 tccgcgtgcg tgagcatcag catcctctgt gggtcgtgca cccacggctc gaacatgttg
     4261 cccacgaact cggggcagcg ctgcaggatg cgcacgaacg agaagccgcg gtg
//
LOCUS       DAOMFD010000168         4269 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9152_length_4269_cov_42.363550, whole genome shotgun sequence.
ACCESSION   DAOMFD010000168 DAOMFD010000000
VERSION     DAOMFD010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9152_length_4269_cov_42.363550"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..148)
                     /gene="rpsE"
                     /locus_tag="PL152_08825"
     CDS             complement(<1..148)
                     /gene="rpsE"
                     /locus_tag="PL152_08825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_004996792.1"
                     /note="located at the back of the 30S subunit body where
                     it stabilizes the conformation of the head with respect to
                     the body; contacts S4 and S8; with S4 and S12 plays a role
                     in translational accuracy; mutations in this gene result
                     in spectinomycin resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HQR49428.1"
                     /translation="MARVEKAAAADEFIEKLVTVNRVAKVVKGGRQFGFTALTVVGDG
                     NGRVG"
     gene            complement(181..531)
                     /gene="rplR"
                     /locus_tag="PL152_08830"
     CDS             complement(181..531)
                     /gene="rplR"
                     /locus_tag="PL152_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HQR49429.1"
                     /translation="MEKTASRQKRARRGRAKIRELKVMRLSVHRTPRHIYAQVFDPQS
                     RVIAVASTVQKDVIAGLEGTGNVEAAKAVGKAIAERAKAKGVTQVAFDRSGFKYHGRV
                     KALAESAREHGLEF"
     gene            complement(542..1075)
                     /gene="rplF"
                     /locus_tag="PL152_08835"
     CDS             complement(542..1075)
                     /gene="rplF"
                     /locus_tag="PL152_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HQR49430.1"
                     /translation="MSRVARKPVTLPQGVTTTVDGDKVTVKGAKGTLALPLRRGLGIV
                     QEGQSVRVEVVEPAHDALMHAGSLRANLANVVEGVSKGYEKKLELVGVGYRAQAQGRN
                     LNLTLGFSHPVVYAVPEGITVETPTQTEILVKGADKQQVGQVAAEIRAYRAPEPYKGK
                     GVRYSGERIELKETKKK"
     gene            complement(1104..1499)
                     /gene="rpsH"
                     /locus_tag="PL152_08840"
     CDS             complement(1104..1499)
                     /gene="rpsH"
                     /locus_tag="PL152_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002215438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HQR49431.1"
                     /translation="MSMTDPIADFLTRIRNGQRSGKPEVGMPASRMKLALAKVLKEEG
                     YIEDFAVATDAGKSTLTLRLKYYQGRPVIDRLERVSRPGLRVYKGKDDLPRILGGMGV
                     AIVSTSRGVMTDREARAGGHGGEILCIVS"
     gene            complement(1521..1826)
                     /gene="rpsN"
                     /locus_tag="PL152_08845"
     CDS             complement(1521..1826)
                     /gene="rpsN"
                     /locus_tag="PL152_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003400428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HQR49432.1"
                     /translation="MAKTSMIEREKRRARLVKQQATKRAALKETIRSPKSTDAEREEA
                     TRKLQSLPRDGSASRIRKRCSITGRSRGVYRKFGLGRQKLREATMRGDVPGLRKASW"
     gene            complement(1838..2377)
                     /gene="rplE"
                     /locus_tag="PL152_08850"
     CDS             complement(1838..2377)
                     /gene="rplE"
                     /locus_tag="PL152_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HQR49433.1"
                     /translation="MARLQTYYREKVVPELMKSLELTNPMRVPKITRITVNMGVGEAV
                     ADKKAMDGAVGDLTALTGQKPLVTKSRKAIASFKLRAGIPVGAKVTLRGARMYEFLDR
                     LINVAMPRIRDFRGVSARSFDGRGNYSFGVKEQIIFPEIAYDKIDAIRGMDITITTTA
                     RDDREGRALLESFNFPFRK"
     gene            complement(2394..2711)
                     /gene="rplX"
                     /locus_tag="PL152_08855"
     CDS             complement(2394..2711)
                     /gene="rplX"
                     /locus_tag="PL152_08855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010486994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HQR49434.1"
                     /translation="MNKIRKGDEVVVIAGREKGRRGTVIRVLANDRVLVENVNMVKRH
                     TRPNPQKGTQGGVVEKEASLHLSNVLLWNPAAKKGDRVGIRTLADGRRVRYFKSNDEV
                     VDA"
     gene            complement(2732..3100)
                     /gene="rplN"
                     /locus_tag="PL152_08860"
     CDS             complement(2732..3100)
                     /gene="rplN"
                     /locus_tag="PL152_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012728338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HQR49435.1"
                     /translation="MIQMRTMLAAADNSGARSLMCIKVLGGSKRRYASVGDVIKVSVK
                     DAIPRGKVKKGEIYDAVVVRTAHGVRRPDGSLIRFDGNAAVLLTNKLEPIGTRIFGPV
                     TRELRTAQFMKIISLAPEVL"
     gene            complement(3123..3410)
                     /gene="rpsQ"
                     /locus_tag="PL152_08865"
     CDS             complement(3123..3410)
                     /gene="rpsQ"
                     /locus_tag="PL152_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HQR49436.1"
                     /translation="MSEPAVAETGAEGAKRTLTGRVVSSKMQKTITVAVERLVKHDKY
                     GKYVRRTTKLLAHDEHGEGREGDLVDIAECRRLSSRKSWRLVRVVRRADQA"
     gene            complement(3407..3622)
                     /gene="rpmC"
                     /locus_tag="PL152_08870"
     CDS             complement(3407..3622)
                     /gene="rpmC"
                     /locus_tag="PL152_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HQR49437.1"
                     /translation="MKAKDIRAKTPAELSEELLKLRREQFNLRMAQASGQATKPDQAG
                     KVRRNIARVKTVQGEQARAAANGSAKR"
     gene            complement(3622..4035)
                     /gene="rplP"
                     /locus_tag="PL152_08875"
     CDS             complement(3622..4035)
                     /gene="rplP"
                     /locus_tag="PL152_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HQR49438.1"
                     /translation="MMQPKRTKYRKQHKGKNGGLAQRGSSVAFGDYGLKAVSRARMTA
                     REIEAARRAMTRYVKRGGQVWIRVFPDVPITKKPLEVRMGSGKGNIEYYVARVQPGKV
                     LFEMEGVTEDIAREAFRLAASKLSVDTLFVKRQVR"
     gene            complement(4067..>4269)
                     /locus_tag="PL152_08880"
     CDS             complement(4067..>4269)
                     /locus_tag="PL152_08880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HQR49439.1"
                     /translation="RTEWSREGRIPLHTFRADIDYGLAEARTTYGVIGVKVWIFKGEV
                     FDQPAQVEAPEAAAAPAQAEPA"
BASE COUNT          524 a         1527 c         1413 g          805 t
ORIGIN      
        1 agccgacgcg gccgttgccg tcgcccacga ccgtgagcgc ggtgaagccg aactgccggc
       61 cgcccttgac gaccttcgcg acgcgattga ccgtgacgag cttctcgatg aactcgtcgg
      121 cggccgcggc tttctcaact ctcgccattg ctgctcctgc ggatcagtgc ggtgtggggc
      181 tcagaattcg agcccgtgct cgcgcgcgga ctcggcgagc gccttgaccc gcccgtggta
      241 cttgaagccg gaccggtcga acgccacctg cgtgacgccc ttggccttgg cgcgctcggc
      301 gatcgccttg ccgaccgcct tggccgcctc gacgttgccc gtgccctcga gccccgcgat
      361 cacgtccttc tgcacggtcg aggccacggc gatcacccgc gactgcgggt cgaacacctg
      421 ggcatagatg tgccggggcg tccggtgcac cgacaggcgc atgaccttca gttcccggat
      481 cttggcgcgt ccgcggcggg cgcgcttctg tcgacttgct gtcttttcca tggcgtcacc
      541 cttacttctt cttcgtttcc ttgagctcga tgcgctcacc ggaataacga acgcccttgc
      601 ccttgtaggg ctccggggca cggtacgcgc ggatctccgc ggcgacctgg cccacctgct
      661 gcttgtccgc gcccttcacc aggatctcgg tctgggtcgg ggtctcgacc gtgatgcctt
      721 ccggcaccgc gtagacgacc ggatgcgaaa agccgagcgt gaggttgagg ttgcggccct
      781 gcgcctgcgc gcggtagccg acgcccacga gctcgagctt cttctcgtag cccttggaca
      841 cgccctccac gacgttggcg aggttcgcgc gcagcgagcc cgcgtgcatc agcgcgtcgt
      901 gcgccggctc gacgacctcg acccgcacgg actgcccttc ctggacgatg ccgaggccgc
      961 ggcgcaacgg cagcgcgagc gtgcccttgg cgcccttcac ggtgaccttg tcaccgtcca
     1021 cggtggtggt cacgccctgt ggcagcgtga ccggctttct tgcgactcgt gacatggtgt
     1081 gaatcctgtt tccggcaccg cgatcaggac acgatgcaga ggatctcgcc gccgtggccg
     1141 ccggcccgcg cctcgcggtc cgtcatcacg ccgcgcgagg tggagacgat ggccacgccc
     1201 atgccgccga ggatgcgcgg caggtcgtcc ttgcccttgt acacgcgcag cccgggacgg
     1261 ctgacgcgct cgaggcggtc gatgaccggg cggccctggt agtacttgag gcgcagcgtc
     1321 agcgtggact tgcccgcgtc ggtggcgacc gcgaagtcct cgatgtagcc ctcttccttc
     1381 agcaccttgg cgagcgcgag cttcatgcgc gacgccggca tgccgacctc cggcttgccc
     1441 gagcgctgcc cgttgcggat gcgggtgagg aagtccgcga tgggatccgt catgctcatg
     1501 tcttgctccg ttacgcggcc ttaccagctg gccttgcgca ggcccgggac gtcgccgcgc
     1561 atggtcgcct cgcgcagctt ctggcggccg aggccgaact tgcggtagac gccacgcgag
     1621 cggcccgtga tcgagcagcg cttgcggatg cgcgacgccg acccgtcgcg cggcaggctc
     1681 tgcagcttgc gcgtcgcctc ctcgcgctcc gcgtccgtgg acttcgggct gcggatcgtc
     1741 tccttgagag cggcccgctt cgtggcctgc tgcttcacga gcctggcgcg gcgtttctcg
     1801 cgctcgatca tgctggtctt cgccattggg cctgccctta cttgcggaac gggaagttga
     1861 aggattcgag cagcgcccgc ccctcacggt cgtcccgcgc cgtcgtggtg atcgtgatgt
     1921 ccatgccgcg gatcgcgtcg atcttgtcgt aggcgatctc ggggaagatg atctgttcct
     1981 tgacgccgaa gctgtagttg ccgcggccgt cgaacgagcg ggccgacacg ccgcggaagt
     2041 cgcggatgcg cggcatcgcc acgttgatca ggcggtcgag gaactcgtac atgcgcgcgc
     2101 cgcgcagcgt caccttggcg ccgaccggga tgccggccct gagcttgaac gacgcgatcg
     2161 ccttgcgcga cttggtgacg aggggcttct ggccggtgag cgccgtgagg tcgcccaccg
     2221 cgccgtccat cgccttcttg tcggcgaccg cctcgcccac gcccatgttg accgtgatgc
     2281 gcgtgatctt cggcacgcgc atggggttgg tgagctcgag gctcttcatg agctcgggca
     2341 cgaccttctc ccggtaatac gtctgcagcc tggccatcag tgcgaacctc gcctcaggcg
     2401 tccacgactt cgtcgttgga cttgaagtag cgcacgcgac ggccgtcggc cagcgtgcgg
     2461 atgcccacgc ggtcgccctt cttcgcggcc gggttccaga gcaggacgtt cgagaggtgc
     2521 agcgaggcct ccttctcgac gacgccgccc tgcgtgccct tctgcgggtt ggggcgcgtg
     2581 tggcgcttga ccatgttcac gttctcgacg agcacgcggt cgttcgcgag cacccggatc
     2641 acggtgccgc gccggccctt ctcgcggccg gcgatcacga ccacctcgtc gcccttgcgg
     2701 atcttgttca tgcctgtttc cccgatcccg ctcagagcac ttccggcgcg agcgagatga
     2761 tcttcatgaa ctgcgcggtg cgcagctcgc gggtgaccgg cccgaagatg cgggtgccga
     2821 tcggctcgag cttgttggtg agcagcacgg cggcgttgcc gtcgaagcgg atcagcgagc
     2881 cgtccggccg gcgcacgccg tgcgccgtgc gcaccacgac cgcgtcgtag atctcgccct
     2941 tcttcacctt gccgcgcggg atcgcgtcct tgacgctcac cttgatgacg tcgcccaccg
     3001 aggcgtagcg gcgcttcgag ccgccgagca ccttgatgca catgaggctg cgcgcaccgc
     3061 tgttgtcggc cgccgcgagc atggttcgca tctggatcat ggtcggtctc ctcgcgcccg
     3121 gctcaggcct ggtcggcgcg tcgcacgacg cgcaccagcc gccacgactt gcggctcgac
     3181 aggcggcggc actccgcgat gtccacgagg tcgccctcgc ggccctcgcc gtgctcgtcg
     3241 tgcgcgagca gcttggtcgt gcggcgcacg tacttgccgt acttgtcgtg cttcacgagg
     3301 cgctcgacgg cgaccgtgat cgtcttctgc atcttgctgc tcacgacgcg tcccgtgagg
     3361 gtgcgcttgg cgccctcggc gcccgtctcc gcgacggctg gctcgctcat ctcttggcac
     3421 tcccgttggc tgccgcgcgg gcctgctcgc cctgcacggt cttcacgcgc gcgatgttgc
     3481 ggcgcacctt gcccgcctgg tcgggcttcg tggcctggcc cgacgcctgc gccatgcgca
     3541 ggttgaactg ctcgcggcgc agcttgagga gttcctcgct gagctccgcc ggagtcttcg
     3601 cccttatgtc cttggccttc atcagcgcac ctgccgcttg acgaacaacg tgtcgaccga
     3661 gagcttgctc gccgccaggc ggaacgcctc gcgcgcgatg tcctcggtga cgccttccat
     3721 ctcgaagagc accttgccgg gctgcacgcg cgcgacgtag tactcgatgt tgcccttgcc
     3781 cgagcccatg cggacctcga gcggcttctt ggtgatcggc acgtccggga acacgcggat
     3841 ccacacctgc ccgccgcgct tcacgtagcg cgtcatggcg cggcgcgccg cctcgatctc
     3901 gcgcgcggtc atgcgcgccc ggctcacggc cttgagcccg tagtcaccga aggcgaccga
     3961 cgagccgcgc tgcgcgaggc cgccgttctt gcccttgtgc tgcttgcggt acttggttcg
     4021 cttgggttgc atcatggtgc tgtctcctct cgcccaggcg cggccctcag gccggctccg
     4081 cctgcgcggg cgcggccgcg gcctcgggcg cctcgacctg cgccggctgg tcgaacacct
     4141 cgcccttgaa gatccacacc ttgacgccga tcacgccgta ggtcgtgcgc gcctcggcga
     4201 gcccgtagtc gatgtccgcg cggaacgtgt gcagcgggat gcggccctcg cgcgaccact
     4261 ccgtgcggg
//
LOCUS       DAOMFD010000169         4267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9159_length_4267_cov_33.855888, whole genome shotgun sequence.
ACCESSION   DAOMFD010000169 DAOMFD010000000
VERSION     DAOMFD010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4267
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9159_length_4267_cov_33.855888"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="PL152_08885"
     CDS             <1..816
                     /locus_tag="PL152_08885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HQR49440.1"
                     /translation="EAGRAQGSFPFQATSVMSPLPRSHHWVDGSAYVNHVELVRKARG
                     AVMPPSFWTDPLVYQGGSDDFLAPMADVPLPSEDHGIDFEGEVAVITDDVPMGTDAAR
                     AAGHIKLVMLVNDWSLRQLIPAELAKGFGFYQSKPATSFSPVAVTPDELGDAWDGGKV
                     LLPLVSHVNGQLFGRPDAGVDMTFDFPRLIEHVTRTRRLGAGAIVGSGTVSNYDRSRG
                     SSCIAERRMLEQLEQGRPLTPFLRFGDRVRIEMFDRSGASIFGAIDQGVVPAA"
     gene            840..2033
                     /locus_tag="PL152_08890"
     CDS             840..2033
                     /locus_tag="PL152_08890"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid aminotransferase"
                     /protein_id="HQR49441.1"
                     /translation="MIFANLEQLPPDPILGVTAAFRADPAADKIDLGVGVYRDERGET
                     PVPEAVREAERAVLAEQRSKTYVGPLGNLEFNERIVALALGPLAAGLRDRTATIQTVG
                     GCGALRLGAELIRASRPDAIVHVSDPTWANHEPLLGTSGLSLQRYPYYDPATRGVDFE
                     RMRDHIASLPSGSIVLLHACCHNPTGADLGQDQWRALGDVIESHGLVPFVDLAYQGFG
                     DDVESDVYGLRHLATRVPQLLLALSCSKNFGLYRERTGALAVVSASISGAAAIATHQA
                     RTARRMYSMPPDHGAAVAARVLGDDGLKAQWITELAAMVARMKGLRALLADKLAQRRP
                     GKDFTWVTCHRGMFSLTGVTASGIVELRDRFHVYVPPDGRINIAGVSEANVDRVADSL
                     SAVLH"
     gene            2077..3378
                     /locus_tag="PL152_08895"
     CDS             2077..3378
                     /locus_tag="PL152_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR49442.1"
                     /translation="MIRLTRYQWTVLLAAWLGWGFDVFDGLLFNYVAPNCVPTLLGLE
                     IGSPEAKQATLFWTGLLTSVLLLGWAAGGILFGMVADRIGRTRTLMLTMLLYALGTAA
                     CAAAPSIAFLVAFRLIASLGIGGEWAAGASMVAEVVPEEQRVEAGALLYTAAPLGLFL
                     ATFVNHQVAAVWMAESPETSWRVVFLFGLVPAAVAFLVRMFVREPERWKAVKQRAPAP
                     TLRDVFAPGVVGVTIRGTLMAIVALISWWSCNAFIPAVSSGLAQAAAQAAGLERGATL
                     ALVESWKTQAMAWFNLGGLIGTLLTIPIAKHLGRKPMFAIYFALSAAAIYAAFGLDWP
                     PETRLHLYFFIGLSVFGIFGSFTFYLPELFPTRLRGTGSGFCYNVGRIVAAAGPFAVG
                     AVAARGVDHALEAISLVALVPLAGLLFVPFIVETRGRVLAD"
     gene            3636..4079
                     /gene="rimP"
                     /locus_tag="PL152_08900"
     CDS             3636..4079
                     /gene="rimP"
                     /locus_tag="PL152_08900"
                     /inference="COORDINATES: protein motif:HMM:NF000927.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HQR49443.1"
                     /translation="MLRDTLTALLRPVVEELGYVLWEVECAAGRGSGFVRLYIDAEAG
                     VTLDDCERVSRAVSEVLDREDPVAGQYTLEVSSPGLERPLRTAAHFARFVGETVFVEL
                     AEPLDGRRRFKGPLLAASGGTIEVDIDGRRYVLPVAGIRKAHLAP"
     gene            4130..>4267
                     /locus_tag="PL152_08905"
     CDS             4130..>4267
                     /locus_tag="PL152_08905"
                     /inference="COORDINATES: protein motif:HMM:NF020118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0031554 - regulation of termination of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NusA N-terminal domain-containing protein"
                     /protein_id="HQR49444.1"
                     /translation="MVVDAVSNEKGVDKEIIFEALEAALASATRKKYGEDIDVRVAIN
                     RK"
BASE COUNT          606 a         1431 c         1509 g          721 t
ORIGIN      
        1 gaagcgggcc gcgcccaggg cagcttcccg ttccaggcca cgagcgtgat gtcgccgttg
       61 ccgcgctcgc accactgggt cgacggatcc gcgtacgtca atcacgtcga actggtgcgc
      121 aaggctcgcg gcgcggtcat gccgccgagt ttctggaccg atccgctcgt ctaccagggc
      181 gggtccgacg acttcctggc gccgatggcc gacgtgccac tcccgagcga ggatcacggc
      241 atcgatttcg aaggcgaagt cgccgtgatc accgacgacg tcccgatggg cacggacgcg
      301 gcacgggccg ccggccacat caagctcgtg atgctggtca acgactggtc gctgcgccag
      361 ctgattcccg cggagctggc gaagggtttc gggttctacc agtcgaagcc agccacgtcg
      421 ttctcgccgg ttgcggtgac tccggacgag ctcggcgacg cctgggatgg aggcaaggtg
      481 ctgctgccgc tcgtctcgca cgtgaacggg cagctgttcg ggcgcccgga cgccggtgtc
      541 gacatgacgt tcgacttccc gcgcctcatc gagcacgtga cgcgcacgcg tcggctcggc
      601 gccggggcca tcgtcggctc cggcacggtg tcgaactacg atcgttcgcg tggatcctcg
      661 tgcatcgcgg agcggcgcat gctcgagcaa ctcgagcagg gcaggccgct gactcccttc
      721 ctcaggttcg gcgaccgcgt gcgcatcgag atgttcgacc ggtccggcgc cagcatcttc
      781 ggcgcgatcg accagggggt cgtgcctgcg gcgtgatctc cgtgcgagaa tccgccgcca
      841 tgatcttcgc caacctcgag cagctgccac ccgacccgat cctcggcgtg acggccgcgt
      901 tccgcgccga ccccgccgct gacaagatcg acctcggcgt cggcgtgtac cgcgacgaac
      961 gcggcgaaac gcccgtgcct gaggccgtgc gcgaggccga gcgcgccgtg cttgcggagc
     1021 agcgcagcaa gacctacgtc ggccccctgg gcaacctcga attcaacgag cggatcgtgg
     1081 cgctcgccct cgggccgctc gcggccggct tgcgcgaccg caccgccacc atccagaccg
     1141 tcggcggctg cggtgcgctg cgtctcggag ccgaactcat ccgggcgtcg cggccggacg
     1201 cgatcgtgca cgtcagtgat ccgacctggg cgaatcacga gccgctgctg gggaccagcg
     1261 gcctgtcact gcagcgctat ccctattacg acccggccac gcgcggagtg gatttcgagc
     1321 ggatgcgcga tcacatcgcc agcctgcctt cgggctcgat cgtgctgctg cacgcgtgct
     1381 gccacaaccc gacgggggcg gacctcggcc aggaccaatg gcgggcgctc ggcgacgtga
     1441 tcgaatccca tggcctggtc ccgttcgtgg acctcgccta ccagggtttc ggcgacgacg
     1501 tcgagtcgga cgtctacggc ctgcggcacc tcgcgacccg agtcccgcaa ctgctgctcg
     1561 ccctgtcgtg ctccaagaat tttggcctct accgcgagcg gaccggggcg ctggcggtgg
     1621 tgtccgcctc gatctcgggc gcagccgcga tcgccaccca ccaggcgcgc acggcgagac
     1681 gcatgtattc catgccgccg gaccacggcg ctgccgtggc cgctcgtgtc ctcggcgatg
     1741 acgggctcaa ggcgcaatgg atcacggagc tcgccgccat ggtcgcacgc atgaaggggc
     1801 tgcgcgcgct gctggccgac aagctggcgc agcgccggcc gggcaaggat ttcacgtggg
     1861 tcacgtgcca ccgcggcatg ttctcgctga ccggcgtgac agcgtccggc atcgtggagc
     1921 tgcgcgaccg gtttcacgtg tacgtgccgc cggatgggcg catcaacatc gccggtgtca
     1981 gcgaggccaa cgtggaccgc gtcgccgaca gcttgtcggc ggtgctgcac tgaagcccgc
     2041 ggcggcgacg cgcgacgagg ccgcggcagg gggaacatga tcaggctgac acgttaccag
     2101 tggacggtgc tgctggccgc atggctcggc tggggcttcg acgtattcga cggcctgctg
     2161 ttcaactacg tcgcgccgaa ttgcgtgccc acgttgctcg gcctggagat cggcagcccc
     2221 gaggccaagc aggcgacgct gttctggacc gggctgctca cgtccgtgct gctgctcgga
     2281 tgggcggcag gcggcatcct gttcggcatg gtcgccgacc gcatcggccg cacgcgcacg
     2341 ctgatgctca cgatgctgct ctacgcgctc ggcacggccg cgtgtgccgc cgcgccgagc
     2401 atcgcgttcc tcgtggcctt ccgcctgatc gcgagcctcg gaatcggcgg cgagtgggct
     2461 gccggagcgt cgatggtcgc agaagtcgtc cccgaggagc agcgtgtgga ggcgggtgcg
     2521 ctgttgtaca cggccgcgcc gctcggcctg ttcctcgcga ccttcgtgaa tcaccaggtc
     2581 gcggccgtgt ggatggcgga gtcaccggag acctcgtggc gggtcgtatt cctgttcggg
     2641 ctggtccctg cggccgtcgc gttcctggtc cgcatgttcg tgcgcgagcc ggagcgatgg
     2701 aaggcggtca agcagcgggc gccggccccg acgctgcgcg acgtgttcgc gccgggagtg
     2761 gtcggcgtga ccatccgcgg cacgctcatg gccatcgtcg cgctgatctc gtggtggagc
     2821 tgcaatgcgt tcatcccggc ggtttcctcc gggctcgcac aggcggcggc gcaggcggcg
     2881 ggcctcgagc ggggcgcgac cctcgcgctc gtggagtcgt ggaagacgca ggccatggcg
     2941 tggttcaacc tcggcggcct gatcggcacg ctgctcacca tcccgattgc gaagcacctc
     3001 ggccgcaagc cgatgttcgc gatctacttc gcgctgtcgg cagccgcgat ctacgcggcg
     3061 tttggcctcg actggccgcc cgaaacgcgg ctccacctgt atttcttcat cggcctgtcg
     3121 gtgttcggca tcttcggcag cttcacgttc tacctgcccg agttgttccc gacgcggctg
     3181 cgcggcaccg gctccggttt ctgctacaac gtcgggcgaa tcgtcgcggc cgccggtccc
     3241 ttcgcggtcg gcgccgttgc ggcacggggg gtggaccacg cgctggaggc catttccctg
     3301 gtcgcgctgg tcccgttggc gggactgctg ttcgtgccgt tcatcgtcga gacgcgcggc
     3361 cgggtgctgg cggactagcc gcagggcgac ccttgctata gaatcccggc tccttgagtc
     3421 ggatccgtat ccaagtcatt gattggattc gattcatggc cgtgcaggca ggcccatgga
     3481 acggggctcg ccggtccggc gggagattcg cctgagaaat ccccctgagc ggggattttt
     3541 catttgcggt gggagcttac gggcgcccgg ccgcgaacca acgaatgggc cgccggccca
     3601 ttttttgttt ttgcgcgttg cgcagggacg ggtgaatgct cagggacacg ctgacggcgc
     3661 tcctgcgacc ggtggtcgag gaactcggct acgtgctctg ggaggtcgag tgcgcggccg
     3721 ggcgcggcag cggcttcgtc cgcctctaca tcgacgcgga ggcgggcgtg acgctcgatg
     3781 actgcgagcg cgtgagccgc gcggtgagcg aggtgctcga ccgcgaggac ccggtggccg
     3841 gccagtacac cctcgaggtg tcgtcgcccg gactcgagcg gccgctcagg accgcggcgc
     3901 acttcgcgcg gttcgtggga gagacggtgt tcgtggaact cgcggagccg ctggacgggc
     3961 ggaggcgctt caagggaccg ctgctcgcag ccagcggcgg gacgatcgag gtcgatatcg
     4021 acgggcggcg ttacgtgctg ccggtcgctg gaatacgcaa ggcgcacctg gcgccctagg
     4081 cttgaacccc gccgagcggg aggacgatgc gatgaacaag gaaatcctga tggtcgtgga
     4141 cgctgtctcc aacgagaagg gcgtcgacaa ggaaatcatc ttcgaggcac tcgaggcggc
     4201 gctcgcgtcg gccacgcgca agaagtacgg cgaggacatc gacgtgcgcg tcgccatcaa
     4261 ccgcaag
//
LOCUS       DAOMFD010000170         4152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9568_length_4152_cov_32.459849, whole genome shotgun sequence.
ACCESSION   DAOMFD010000170 DAOMFD010000000
VERSION     DAOMFD010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4152
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9568_length_4152_cov_32.459849"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..590)
                     /locus_tag="PL152_08910"
     CDS             complement(<1..590)
                     /locus_tag="PL152_08910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017391359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HQR49445.1"
                     /translation="MVNVHKKSFSYGPHTVTLETGRLARQADGAVLVTMGDTSVLVTA
                     VGRKDADPNKDFFPLTVNYVEKTYAAGRIPGGFFKREGRPSEKETLTSRLIDRPIRPL
                     FPEGFFNEVQVVATVVSLDPDIDSDIPAMLGASAALALSGIPFKGPIGAAKVGYRDGQ
                     YLLNPTAKDLQTSKLDLVVAGTADAVLMVESEAKMLP"
     gene            complement(667..936)
                     /gene="rpsO"
                     /locus_tag="PL152_08915"
     CDS             complement(667..936)
                     /gene="rpsO"
                     /locus_tag="PL152_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HQR49446.1"
                     /translation="MALSTERKGEIVGGYRRNTKDTGSPEVQIALLSARINELGEHFT
                     THKRDHSSRRGLLKLVTQRRKLLDYLKASDPARYQDVVSRLGLRR"
     gene            complement(1008..1931)
                     /gene="truB"
                     /locus_tag="PL152_08920"
     CDS             complement(1008..1931)
                     /gene="truB"
                     /locus_tag="PL152_08920"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HQR49447.1"
                     /translation="MDGIVLLDKPPGMSSNQALQRVRRLLAAAKGGHAGTLDPMATGM
                     LPLCFGQATKACGQLLGSRKAYRATIALGAATDTGDADGAITVEADVPTLDQSRVEAV
                     LARFTGTIEQIPPMYSALKRDGRPLYELARRGEDVPRETRHVVIHRLSLASLAARELS
                     IEVICAKGTYVRALGEDIARALGTCGHLSDLRRLWVEPFEGEPMVALEDVERWAAEGG
                     AEPGRPPWLMPVDRAFAGLPRLDLDPTGARHLCQGRVLHSAAGTTAVGTARAYGADGR
                     FLGLVSIDGSGVVRVARLFLAGEGSAGSLHA"
     gene            complement(1982..2383)
                     /gene="rbfA"
                     /locus_tag="PL152_08925"
     CDS             complement(1982..2383)
                     /gene="rbfA"
                     /locus_tag="PL152_08925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HQR49448.1"
                     /translation="MPREVPRARRVAEQVQRTLAEMLRREVRDPRLKPLTITHVRVSP
                     DLTHAWVHYALLSGDSHDPLQAEILEEASRQLRGPLGRALRLRVAPQLHFQPDEELER
                     GNRLDDLITQAVREDRTRHVDEGGAVEDEGE"
     gene            complement(2393..>4152)
                     /gene="infB"
                     /locus_tag="PL152_08930"
     CDS             complement(2393..>4152)
                     /gene="infB"
                     /locus_tag="PL152_08930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HQR49449.1"
                     /translation="TAPVKREVQIPETITVGELAGKMAVKANEVIKTMMKMGVMATIN
                     QPIDQDTAQLVVEEFGHSAVRLKESALEDQLQATAAEVEALPRPPVVTIMGHVDHGKT
                     SLLDYIRRTKVAAGEAGGITQHIGAYQVETPKGLITFIDTPGHAAFTAMRARGAHVTD
                     IVVLVVAADDGVMPQTVEAIQHARAANVPIVVAVNKIDKSDADPDRVRNDLAKQNVIP
                     EDWGGDTLFVHVSARTGEGIDQLLETILLQAEVLDLKAPRDGLAVGVVLESSIEKGRG
                     PVATVLVKRGTLKSGDPIIAGQEFGRVRALFDATGKQVQEAGPAVPVAVLGLSDAPNA
                     GDDLLVVDSERKAREVALYRQGKFRDVRLAGAAKKAEDVFSQMGEQAGAVVSLIVKAD
                     VQGSAEALRDAFTKLSTEEVVVKVIASGVGGITESDVTLAAASKARIIGFNVRADGAA
                     RNAVKDTGVDVHYYSIIYEAIDDVKQAMTGLLKPEIKEQIVGLAEVREVFRSSKFGTV
                     AGCLVVEGYIKRGNPVRVLRDNVVIHQGELDSLRRFKDDVGEVRAGTECGIGVRNYND
                     IRAGDQIECYSRVEVARSA"
BASE COUNT          585 a         1464 c         1409 g          694 t
ORIGIN      
        1 ggcagcatct tcgcctcgga ctcgaccatc agcaccgcgt cggccgtgcc ggcgacgacg
       61 aggtcgagct tggaggtttg caggtccttc gccgtgggat taagcaggta ctggccgtca
      121 cggtagccca ccttggcagc gccgatcggc cccttgaacg ggatgcccga gagtgcgagt
      181 gccgcggaag cgccgagcat cgccgggatg tccgaatcga tgtccgggtc gagcgacacg
      241 acggtcgcaa ccacctgcac ctcgttgaag aagccctcgg ggaacaacgg gcggatcggc
      301 cggtcgatga ggcgggaggt cagggtttcc ttttccgaag gacgtccttc gcgcttgaag
      361 aacccgcccg ggatgcggcc cgcggcatag gtcttctcga cgtagttgac ggtcagcggg
      421 aagaaatcct tgttggggtc ggcgtccttg cggcccaccg cggtcacgag gaccgaggtg
      481 tcccccatgg tgacgaggac cgcgccgtcc gcctggcggg cgaggcgccc ggtctcgagg
      541 gtgactgtgt gcggaccgta ggaaaaagac ttcttgtgga cgttcacgat ggtgtcccgt
      601 gctgcagagc gtgtgtgagg tgccgaaaac gaatccggcc cgctcagcgg gccgggattc
      661 ggggagtcag cggcgcaggc ccagccggct cacgacgtcc tggtagcggg ccgggtcgga
      721 cgccttcagg tagtcgagga gcttgcggcg ctgggtgacg agcttcagca ggccacgacg
      781 cgaggagtgg tcgcgcttgt gcgtggtgaa gtgctcgccg agttcgttga tgcgggctga
      841 cagcagggca atctggactt ccggcgagcc ggtgtccttc gtgttgcgac ggtagccgcc
      901 gacgatctcg cccttgcgct cggtggacag tgccattctc tgggatcctc gaaaaccttg
      961 tatgcctttg gtgagccgcg tattttacct gtcggcggac cagaatctca agcgtgcagc
     1021 gaacccgctg agccctcgcc ggcgaggaac agcctcgcga cccgaaccac gcccgacccg
     1081 tcgattgaga cgaggccgag gaaccggcca tcggccccgt acgcgcgcgc cgtgcccacg
     1141 gccgtggtcc ccgccgcact gtgcaggacc cgcccctggc acaagtgccg cgcaccggtg
     1201 ggatccaggt ccagtcgcgg cagtccggca aaggcccggt ccaccggcat gagccagggt
     1261 ggccggccgg gttcggcccc gccctccgcc gcccagcgct cgacgtcctc gagcgcgacc
     1321 atcggttcgc cctcgaacgg ctcgacccac agtcgccgca ggtcgctcaa gtgcccgcag
     1381 gtacccagcg cacgggcgat gtcctcgccg agggcgcgca cgtacgtgcc cttcgcgcag
     1441 atcacctcga tcgagagttc ccgggcagcc agcgacgcca gcgacaggcg atgaatcacg
     1501 acgtgccggg tctcgcgggg cacgtcctcg ccacggcgcg ccagctcata gagtggacgc
     1561 ccgtcccgct tgagcgcgga atacatgggc gggatttgct cgatcgtgcc ggtgaagcga
     1621 gccaggaccg cctcgacccg cgactggtcg agtgtcggga cgtccgcctc cacggtgatg
     1681 gcgccgtcgg catcgccggt gtcggtggcg gccccgagcg cgatcgtcgc gcggtacgcc
     1741 ttccgcgatc ccagcagctg cccgcaggcc ttggtcgcct gcccgaagca cagcggcagc
     1801 atcccggtcg ccatgggatc gagcgtgccg gcgtgcccgc ccttggcggc cgcgagcagc
     1861 cggcggacgc gctgcagcgc ctggttcgag ctcatgccgg gcggcttgtc gagcaggacg
     1921 attccgtcca cgtcgcgcca gcggcggcga tgaggaccgc ccaatgcact gccgggctcg
     1981 ctcactcgcc ctcgtcttcg accgccccgc cctcgtcgac gtgccgcgtg cggtcctcgc
     2041 gcacggcctg cgtgatcagg tcatcgagcc ggttgcctcg ctcgagctcc tcgtccggct
     2101 ggaagtggag ctgcggcgcg acccggagcc gcagcgcgcg gccgagcggt ccgcgcagct
     2161 gccgagaagc ttcttcgagg atctccgcct gcagcggatc gtgcgagtct ccagacagca
     2221 gcgcgtagtg cacccacgcg tgcgtcaggt ccggcgacac gcgcacgtgc gtgatcgtga
     2281 gcggtttcag ccgcgggtca cgcacctcgc ggcgcagcat ctccgccagg gtgcgctgca
     2341 cctgctcggc cacgcggcgc gcacgcggaa cttcacgcgg catgcgctgt gctcacgcgc
     2401 tgcgcgcgac ctcgacccgc gagtagcact cgatctggtc gcccgcgcgg atgtcgttgt
     2461 agttacgcac gccgatgccg cactcggtgc ccgcccgcac ctcgccgacg tcgtccttga
     2521 agcgtcgcag cgagtcgagt tcgccctggt ggatcacgac gttgtcgcgc agcacgcgca
     2581 ccgggttgcc gcgcttgatg tagccctcga ccacgaggca tcccgccacg gttccgaact
     2641 tgctggaccg gaacacctcg cgcacctccg cgaggccgac gatctgctcc ttgatctcgg
     2701 gcttgagcag gccggtcatc gcctgcttca cgtcgtcgat ggcctcgtag atgatgctgt
     2761 agtagtgcac gtcgacgccg gtgtccttca cggcattgcg ggcggcgccg tcggcgcgca
     2821 cgttgaagcc gatgatgcgc gccttcgagg ccgccgccag cgtgacgtcg gattcggtga
     2881 tcccgcccac gccgctcgcg atcaccttga ccacgacctc ctcggtcgag agcttggtga
     2941 acgcgtcgcg cagcgcctcg gcgctgccct gcacgtccgc cttcacgatc agcgacacga
     3001 ccgcgccggc ctgctctccc atctgcgaga acacgtcctc ggctttcttc gcagctccgg
     3061 cgagccgcac gtcgcggaac ttgccctgcc ggtacagcgc gacctcgcga gccttgcgct
     3121 cgctgtcgac gaccagcagg tcgtcgccgg cgttcggcgc gtcggacagg ccgagcaccg
     3181 cgaccggcac cgccgggccc gcctcctgga cctgcttgcc ggtcgcgtcg aacagggcgc
     3241 gcacgcggcc gaattcctgg cccgcgatga tcgggtcgcc cgacttgagc gtgccgcgct
     3301 tgacgagcac cgtcgcaacc ggtccgcggc ccttctcgat gctcgactcg agcaccacgc
     3361 cgaccgcgag gccgtcgcgc ggcgccttga ggtccagcac ctcggcctgc agcaggatcg
     3421 tctcgagcag ctggtcgatg ccttccccgg tgcgcgccga gacgtgcacg aacagcgtgt
     3481 caccgcccca gtcctcggga atcacgttct gcttcgcgag gtcgttgcgc acgcggtccg
     3541 ggtccgcgtc gctcttgtcg atcttgttga ccgcgaccac gatcggcacg ttggccgcac
     3601 gggcgtgctg gatcgcctcg accgtctgcg gcatgacacc gtcgtcggca gcgacgacca
     3661 gcaccacgat gtccgtgacg tgcgcgccgc gtgcgcgcat cgcggtgaac gcggcgtgac
     3721 ccggcgtgtc gatgaaggtg atcaggcctt tcggcgtctc gacctggtag gcaccgatgt
     3781 gctgggtgat gccgccggcc tctcccgccg ccaccttggt gcggcggatg tagtcgagca
     3841 gcgaggtctt gccgtggtcg acgtggccca tgatggtcac gaccggcggc cgtggcagcg
     3901 cctcgacctc ggcagccgtc gcctgcaact ggtcctcgag ggcgctctcc ttcaggcgca
     3961 cggccgagtg gccgaattcc tcgaccacca gctgcgcggt gtcctggtcg atgggctggt
     4021 tgatggtggc catcacgccc atcttcatca tggtcttgat gacctcgttg gccttcaccg
     4081 ccatcttgcc ggccagttcg ccgaccgtga tcgtctccgg gatctgcacc tcgcgcttga
     4141 ccggcgcggt gg
//
LOCUS       DAOMFD010000171         4133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9652_length_4133_cov_34.946052, whole genome shotgun sequence.
ACCESSION   DAOMFD010000171 DAOMFD010000000
VERSION     DAOMFD010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4133
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9652_length_4133_cov_34.946052"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /gene="pilM"
                     /locus_tag="PL152_08935"
     CDS             complement(<1..595)
                     /gene="pilM"
                     /locus_tag="PL152_08935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HQR49450.1"
                     /translation="MLFQGRSRPVIGLDITTSSIKLIELSRSGDGYRVESYAAEPAPP
                     NAINEKAIVDSQAVGEAVRRAVKRAGARSKECAVAISGDAAITKVIQMPRNLRDAELE
                     SQVEMQADQYIPFPMEEVSFDFEVIGPSEKDPDMLDVLLVATRSENVEQRQAAVHAAG
                     LATRIVDVEAFALENACRLLTHQMPDGGMDRNIAVIDF"
     regulatory      complement(879..962)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01051"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cyclic di-GMP riboswitch class I; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="cyclic di-GMP"
                     /db_xref="RFAM:RF01051"
     gene            1019..3523
                     /locus_tag="PL152_08940"
     CDS             1019..3523
                     /locus_tag="PL152_08940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020647835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HQR49451.1"
                     /translation="MQRPVGKLLATLLTAVLAIAGAVVLGIAGAFQFVEPDLPDAAEL
                     RDLRMQLPLTVYSRDGKLIAQIGEQRRIPVPRADMPPQLVDAFLAAEDDRFFEHPGVD
                     WQGLVRALAANVSAGGVREGGGTITMQLARNTLLTPERTLRRKLKEVFLALSIEREFT
                     KDEILTQYLNRIFLGQRAYGVGAAAEVYFGKKVGELTLGEAALIAGLPRSPSRDNPAA
                     SVARAHARRTYVLRRMAETGTIDEAAREAADSESISPRIHAPVVQVQAPYVAEMVRQE
                     MLRRYGPSALTDGYAVTTTVDSVLQAAADSALWRAMLEYDRRHGYRGPAGRVDPAALD
                     DAASVAAAFAQHPGQGSLHVAVVASVGLREARVRTRDGRAIDLDWDAVAWARPALPDA
                     AVGPAPKSAGDVLAIGDIVFVEARGDGTYRLAQVPAVGAAFTALDPHDGAILALVGGF
                     DFWASKYNRAVQAKRQPGSAFKPFLYSAALEHGFTPATLINDAPIVLPGGGGDDGTEE
                     WRPQNITKKFYGPTPMREALVRSRNLVSIRLLRGTGLGPAMRHIGAFGFGPEAMPPNL
                     TLALGTGQVTPLEMARGFSVFANGGWLVTPYFIASVDDASGTTVFQATPPLACPQCVD
                     EPQVDAAASTADASPAASPPTPIVDGSAAIAAGGDQGLVPPERRATRAISAANAYIMT
                     DIMTDVIQRGTAVRARSLGRRDIAGKTGTTSDHRDAWFAGFNADLAAVAWIGFDQERS
                     LGDNEEGARAALPMWIYFMEQALQGRPEHRLPEPPGVVRMWVSRETGAPASAGSPDAI
                     FEAFLERYVPQPGQSGYDVEGVDEESVQPSTDDSIF"
     gene            3535..>4133
                     /locus_tag="PL152_08945"
     CDS             3535..>4133
                     /locus_tag="PL152_08945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49452.1"
                     /translation="MHRRPNPRAELLRLAVAEEAARIMREQGVEDFLQAKRKAAERLG
                     VTDAAILPRNTEIEAAKIAHQRLFAADRHEADLASLRRAALEAMRLVSDFEPRLVGPV
                     LAGTASTHSEINLHVFSETPEAVSIRLEERGVPHEVVERRLRYERDRTVVYPAVRFVA
                     GRQTVDAVVFPVDGIRQSPASPVDGKPMRRASAAEVAALL"
BASE COUNT          615 a         1462 c         1460 g          596 t
ORIGIN      
        1 cgaagtcgat gacggcgatg ttgcggtcca tgccgccgtc gggcatctgg tgcgtcagca
       61 ggcggcacgc gttctcgagc gcgaaggcct cgacgtccac gatgcgggtc gccagcccgg
      121 cagcgtgcac cgcggcctgc cgctgctcga cgttctccga gcgcgtcgcg acgagcagga
      181 cgtcgagcat gtccgggtcc ttctccgaag ggccgatcac ctcgaaatcg aagctgacct
      241 cctccatcgg gaaggggatg tactggtccg cctgcatctc gacctgcgac tcgagttccg
      301 cgtcgcgcag gttgcgcggc atctggatga ccttggtgat cgccgcgtcg ccgctgatcg
      361 cgaccgcgca ttccttgctg cgcgcgccgg cgcgcttcac ggcgcggcgc accgcctcgc
      421 cgacggcctg cgaatcgacg atcgccttct cgttgatggc gttcggcggc gcgggctccg
      481 ccgcgtacga ctcgacgcga taaccgtcac cactccgcga aagctcgatg agcttgatcg
      541 acgacgtcgt gatgtcgagt cctatgactg gtcgtgaccg gccctgaaac agcacgagtg
      601 agtccttcta gttcaagagg ttgcgctgcg atcgtggagt gaattccatg cccccgccgc
      661 actatataac agggatccgc caaaaagaat tgtgagcggc ctagcagaat ccagcatctg
      721 gcagcggaac ccccctgtgc gatgtgtcga tggtcacacg ccacccccgg tcgagacacc
      781 agccggcgcc gcgggccagg cttccgggaa cggggtccag tcgctcgacg atcggtacgc
      841 tgctgcgccg tgaacacgag cgcggcggca tcgtccggac cagcaacccc gctgtcatag
      901 gcctcaccct tagaccggtg gctttgcgtc cccgcctttc ggcgggtttg cccttgcgtc
      961 gcgagctact atgcacaacc gccgcaggcg cggcaaggac tggttcccag aattcggcat
     1021 gcagcgacca gtgggaaaac ttctcgcgac actgctcacg gcagtgctcg ccatcgcagg
     1081 cgcggtcgtg ctcggcatcg cgggcgcatt ccagttcgtc gagcccgacc tgccggatgc
     1141 cgccgagctg cgcgacctgc gcatgcagct gccgctcacg gtgtactcgc gcgacggcaa
     1201 gctgatcgcc cagatcggcg agcagcggcg catcccggtg ccgcgcgcgg acatgccgcc
     1261 gcagctggtc gacgcattcc tcgccgccga ggacgatcgc ttcttcgagc atccgggcgt
     1321 ggactggcag ggactcgtgc gcgcgctcgc ggccaacgtc tccgcgggcg gcgtgcgcga
     1381 gggcggcggc accatcacca tgcagctcgc acgcaacacg ctgctcacgc cggagcgcac
     1441 gctcaggcgc aagctgaagg aagtgttcct cgcgctcagc atcgagcgcg agttcaccaa
     1501 ggacgagatc ctgacgcagt acctgaaccg catcttcctc ggccagcgcg cgtacggcgt
     1561 cggcgcggcc gccgaggtgt acttcggcaa gaaggtcggc gagctgacgc tcggcgaagc
     1621 ggcgctcatc gccggcctgc ctcgctcccc gtcgcgcgac aatcccgcgg ccagcgtcgc
     1681 gcgggcgcac gctcgccgca cctacgtgct gcgccgcatg gccgagaccg gcacgatcga
     1741 cgaggcggcg cgcgaggctg cggatagcga gtccatcagc ccgcgcatcc acgcaccggt
     1801 cgtgcaggtg caggcgccgt acgtcgccga gatggtgcgg caggagatgc tgcgccgcta
     1861 cggcccgagc gcgctgaccg atggatacgc cgtcacgacg acggtcgaca gtgtcctgca
     1921 agcagcggcg gactccgcgc tgtggcgtgc catgctggaa tacgaccggc ggcacgggta
     1981 tcgcggcccg gccggccggg tcgatcccgc cgcgctcgat gacgcggcgt cggtcgcggc
     2041 cgcgtttgcc cagcaccccg gccagggaag cctgcacgtc gcggtcgtcg cgtcggtcgg
     2101 gctgcgcgag gcgcgggtgc gcacgcgcga cggccgggcg atcgacctcg actgggatgc
     2161 ggtggcgtgg gcacgaccgg cgctgccgga tgcggccgtc ggcccggcgc cgaagagcgc
     2221 cggcgacgtg ctggccatcg gcgacatcgt gttcgtggag gcgcgcgggg acgggacgta
     2281 tcgcctcgca caggtgccgg ccgtgggcgc cgccttcacc gccctcgatc cgcacgacgg
     2341 tgcgatcctc gccctggtcg gcggcttcga cttctgggcg agcaagtaca accgcgccgt
     2401 gcaggcgaaa cgccagccgg gctcggcatt caagccgttc ctgtactccg ccgcgctcga
     2461 gcacgggttc accccggcga cgctgatcaa cgacgcgccg atcgtgctgc cgggcggcgg
     2521 cggtgacgat ggcacggagg aatggcggcc ccagaacatc accaagaagt tctacggccc
     2581 gacgccgatg cgcgaggcgc tggtgcgttc gcgcaacctc gtctccatcc gcctgctgcg
     2641 cgggaccggc ctcggcccgg cgatgcggca catcggcgcg tttggcttcg gccccgaggc
     2701 catgccgccc aacctgaccc tcgcgctcgg gaccgggcag gtgacgccgc tcgagatggc
     2761 gcgcggcttc tcggtgttcg cgaacggcgg ctggctcgtg acgccgtact tcatcgcgag
     2821 cgtggacgat gcgtcgggca caacggtttt ccaggccacg ccaccgctcg cctgcccgca
     2881 gtgcgtggac gaaccgcagg tcgatgccgc ggcgagcacg gctgacgcct cgcccgccgc
     2941 gtcgccgccg accccgatcg tcgacggatc ggcggccatc gctgccggcg gggaccaggg
     3001 cctggtcccg ccggaacgca gggcgacccg ggcgatctcg gccgccaacg cctacatcat
     3061 gaccgacatc atgaccgacg tgatccagcg cggcaccgcg gtgcgcgccc gttcgctcgg
     3121 ccggcgggac atcgccggga agaccggcac gaccagcgac caccgcgacg cgtggttcgc
     3181 gggattcaac gcggacctgg ccgcggtcgc gtggatcggc ttcgaccagg agcgctcgct
     3241 gggcgacaac gaggaaggcg cgcgcgccgc gctgccgatg tggatctact tcatggaaca
     3301 ggcgctgcag ggccgtccgg agcaccggtt gccggagccg ccgggcgtgg tgcgcatgtg
     3361 ggtgtcgcgc gagacgggcg cgccggcgag cgccggcagc cccgacgcga tcttcgaggc
     3421 gttcctcgag cggtacgtac cgcagccggg ccagtccggc tacgatgtcg agggcgtgga
     3481 cgaggagagc gtccagccgt cgaccgacga ttcgatcttc tgagcaggcc gccgatgcat
     3541 cgacgaccga acccccgcgc cgaactgctg cgccttgccg tcgccgagga agccgcacgc
     3601 atcatgcgcg agcagggcgt cgaggatttc ctgcaggcga agcgcaaggc cgccgagcgg
     3661 ctcggcgtga cggacgccgc catcctgccg cgcaacacgg agatcgaggc cgcgaagatc
     3721 gcgcaccagc ggctgttcgc ggcggaccgg cacgaggcgg acctagcgtc gctgcgccgg
     3781 gccgcgctcg aggcgatgcg gctcgtttcg gacttcgagc cgcgcctggt cgggcccgtg
     3841 ctcgccggga cggcgtccac ccactccgag atcaacctgc acgtcttctc ggagacgccg
     3901 gaggccgtct ccatccggct cgaggagcgc ggggtgccgc acgaggtggt cgagagaagg
     3961 ctgcgctatg aacgcgaccg cacggtggtc tacccggccg tgcgcttcgt cgcgggccgg
     4021 cagaccgtgg acgcggtggt gttcccggtg gatgggatcc gccagtcccc ggcgagcccg
     4081 gtggacggca agccgatgcg gcgcgccagc gccgcggaag tcgcggccct gct
//
LOCUS       DAOMFD010000172         4091 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9809_length_4091_cov_35.756690, whole genome shotgun sequence.
ACCESSION   DAOMFD010000172 DAOMFD010000000
VERSION     DAOMFD010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4091)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4091
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9809_length_4091_cov_35.756690"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="PL152_08950"
     CDS             <1..718
                     /locus_tag="PL152_08950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49453.1"
                     /translation="RLPHNGFFRWVTDMLFPTLLRISSWFPDKVLLLNFTHARFLGYE
                     VAAFPYGIDVNPKDGSIWYAKLYANKIGRVDPKTLEVKEYDTPMSGPRRPRFDKDGIF
                     WIPAFDEGGLMRFDPATGTFESYKIPGVAPGEYETPYALNVYRPTGDVWLAPNNSDRI
                     VKFTPATKTFQSYPSPTRVTVLRDFSFTKDGRVCSSQSNLPAYAIEGGVPSYLCIDPT
                     GGERDRAALAAVSTPPAVPR"
     gene            complement(811..2526)
                     /locus_tag="PL152_08955"
     CDS             complement(811..2526)
                     /locus_tag="PL152_08955"
                     /inference="COORDINATES: protein motif:HMM:NF002505.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClcB-like voltage-gated chloride channel
                     protein"
                     /protein_id="HQR49454.1"
                     /translation="MTRARLLFVGHEELIGLVFWAALTGFLGALASVLFREGIRLFEW
                     LLTGETSGLVQAASSLHGWHRVVTPVIGGVAAGAILHYGSRLIGKRRSIDYMEAVAVG
                     DGTIAARPTIVHSVSSLLTIGSGGSIGREGPMVQLAALIGSKLGFLSQAPVPRRRLLV
                     ACGAAAGISAAYNAPIAGALFVAEIVLGSIAMESFGPLLVASVTANATIHRFLGYGPV
                     FAVPHMQFGSNWELVFYGLLGVLLGHLAPLFLGLLEWARHMFGKLRLPLYVKLGLGGL
                     VVGVVSLALPEVWGNGYSVVSRILNGQLLGWMLLAVLLAKVVTTAATVGSGAVGGIFT
                     PTLFVGAAVGAASGALLHLVLPAASSSPGAYALVGMGGLLAATTHAPLMSILMIFEMT
                     LDYEVVLPLMLACVTAHFTAKAYRQGRSVYEESLSRDAVTGEPEWHLRTVEALVKPAA
                     AVVQEHATLRRMLDELPRRPLRTVYVVDEGQQLLSSIELPQLLDRIRKGEVDVDLAVG
                     TLATPVSFVLTPDMTLTTALDGFLRMQAKTLPVTAGHWHTTLLGEVSRHDLLLALQDR
                     IGAKG"
     gene            complement(2608..3951)
                     /gene="accC"
                     /locus_tag="PL152_08960"
     CDS             complement(2608..3951)
                     /gene="accC"
                     /locus_tag="PL152_08960"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HQR49455.1"
                     /translation="MLDKVVIANRGEIALRILRACRELGIKTVAVHSTADRNLKHVLL
                     ADETVCIGPPPSRESYLNMPAIISAAEVTDAVGIHPGYGFLSENADFAERVEKSGFTF
                     IGPRAETIRLMGDKVSAIQTMKKAGVPCVPGSDGPLGTEPDEIIRVARDIGFPVIIKA
                     SGGGGGRGMRVVHSEATLIHSVGVTRAEALAAFGNDQVYMEKFLERPRHIEFQVLADH
                     YGNVICLGERDCSMQRRHQKVIEEAPAPGLTAKQRKHMSDRVAAACADIGYRSAGTLE
                     FLYQDDEFYFIEMNTRIQVEHPVTEIITGIDLVKAQLRIAAGERLQWSQDDVHIRGHA
                     IECRINAEDPKKFTPSPGPVKLWHAPGGPGVRVDSHVYTGYNVPPHYDSLIAKVIAFG
                     EDRDTAIARMRNALGEIVVEGIKTNVPLHQEIFQHAAFRAGGTDIHYLERRLGLK"
     gene            complement(3951..>4091)
                     /locus_tag="PL152_08965"
     CDS             complement(3951..>4091)
                     /locus_tag="PL152_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HQR49456.1"
                     /translation="PGDVLCIIEAMKMMNQIECDKAGKVVSILVKNGDPVEFGQPLFV
                     IE"
BASE COUNT          679 a         1393 c         1380 g          639 t
ORIGIN      
        1 gcggctgccg cacaacggct tcttccgctg ggtcacggac atgctgttcc cgacgctgct
       61 caggatctcg tcgtggttcc ctgacaaggt gctgctgctc aacttcacgc acgcccgctt
      121 cctgggttac gaggtcgccg cgttcccata cggaatcgac gtgaatccga aggacggcag
      181 catctggtac gcgaagctgt acgcgaacaa gatcgggcga gtcgatccaa agaccctcga
      241 ggtcaaggag tacgacacgc cgatgagcgg tccgcgccgc ccgcgcttcg acaaggacgg
      301 catcttctgg atcccggcct tcgatgaagg cgggttgatg cggttcgatc cagcgacggg
      361 aacctttgag agctacaaga tccccggcgt cgccccgggc gagtacgaaa cgccctatgc
      421 actcaacgtc taccggccga cgggcgacgt ctggctggcg ccgaacaact cggatcgcat
      481 cgtgaagttc acgcccgcga cgaagacgtt ccagagctac ccgagcccga cccgcgtcac
      541 ggtgttgcgc gatttctcgt tcacgaagga cggccgggtc tgctcatcgc aatcgaacct
      601 gccggcctac gcgatcgagg gcggtgtacc gagttacctc tgcatcgacc cgacgggcgg
      661 ggagcgcgat cgcgcggcgc tggcggccgt ttcgacgccg ccggccgttc cgcggtagcc
      721 ggggccggac cgccgatttc tcctgcttcg ggcctcagcc gccgctcgac gtggtggatg
      781 tcggtaccgc cggcgcggat cgcgcaggct tcagcccttg gcgccgatcc tgtcctgcag
      841 cgcgagcagg aggtcgtggc gcgagacctc gccgagcagc gtcgtatgcc agtgcccggc
      901 ggtgaccggg agggtcttcg cctgcatgcg caggaacccg tccagcgccg tcgtcagcgt
      961 catgtccggc gtcaggacga acgacacagg cgtcgcgagc gtgccgacag ccaggtcgac
     1021 gtcgacctct cccttgcgaa tcctgtcgag cagctgcggc agttcgatcg acgacagcag
     1081 ttgctgtccc tcgtcgacca cgtagacggt acgcagcgga cgcctcggca gttcgtcgag
     1141 catccggcgc agcgtcgcgt gctcctgtac gacggcggcc gccggcttga ccagcgcctc
     1201 gaccgtgcgc aggtgccatt ccggttcccc ggtcacggcg tcgcgcgaca gcgactcctc
     1261 gtagaccgag cggccttggc gatacgcctt cgcggtgaag tgggccgtca cgcaggcgag
     1321 catgagcggc agcacgacct cgtagtccag cgtcatctcg aagatcatca ggatcgacat
     1381 gagcggcgcg tgcgtggttg ccgccagcag cccgcccatg ccgacgagcg cataggctcc
     1441 gggcgaagag gatgccgcgg gcaggacgag gtgcagcagc gcgcccgacg ccgcgccgac
     1501 cgcggcgccc acgaacagcg tcggcgtgaa gatgccgccg accgcgccgg agccgaccgt
     1561 ggccgccgtc gtgaccacct tggccagcag gacggccagc agcatccagc ccagcagctg
     1621 accgttcagg atcctcgaga ccacgctgta gccattgccc cagacttccg gcaaggcgag
     1681 tgagaccacg ccgacgacca ggccgccgag cccgagcttc acgtacaacg gcaggcgcag
     1741 cttgccgaac atgtgcctgg cccattcgag caggccgagg aacaacggcg cgagatgtcc
     1801 gagcagcacg ccgagcaggc cgtagaagac cagctcccag ttcgaaccaa actgcatgtg
     1861 cggcacggcg aacaccgggc cgtagccgag gaagcggtgg atcgtggcgt tggcggtgac
     1921 cgacgcgacc agcagcggac cgaaactctc catcgcgatc gagccgagga cgatctccgc
     1981 gacgaacagt gcgccggcga tcggcgcgtt gtaggcggcg gaaatgccgg ccgcggcgcc
     2041 gcaggcgacc agcagccggc gccgcggtac cggagcctgg gaaaggaagc cgagcttcga
     2101 accgatcagg gccgcgagct gcaccatcgg accttcgcgg ccgatcgagc cgccggaccc
     2161 gatcgtgagc agcgacgaca cgctgtggac gatcgtcggt cgggcagcga tggtgccgtc
     2221 gccgaccgcc acggcctcca tgtagtcgat cgaccgacgc ttcccgatga gcctggagcc
     2281 atagtgcagt atcgctcccg ccgccacgcc gccgatcacg ggcgtgacca cccggtgcca
     2341 gccgtggagc gaactggccg cctgtacgag ccccgacgtc tcgccggtca gcagccactc
     2401 gaagagacgg atgccttcgc ggaacagcac gctcgcgagc gcgccgagaa agccggtcaa
     2461 ggcggcccag aacaccaggc cgatgagctc ctcgtgcccg acgaacagga gtctcgcccg
     2521 ggtcaaccag cgaatcggcc gacgcgcgag ggattcaatg cggccacccg ccatcgtccc
     2581 tgctcctcag gccatggggg tgcggcacta cttcaggccg agccgccgct cgaggtagtg
     2641 gatgtcggtg ccgccggcgc ggaacgcggc gtgctggaag atctcctggt gcagcggcac
     2701 gttggtcttg atgccctcga cgacgatctc gccgagcgca ttgcgcatgc gggcgatcgc
     2761 cgtgtcgcgg tcctcgccga aggcgatgac cttggcgatg agcgaatcgt agtgcggcgg
     2821 cacgttgtag cccgtgtaca cgtggctgtc gacgcgcacg ccggggccgc ccggcgcgtg
     2881 ccagagcttc acgggacccg gtgacggcgt gaacttcttg gggtcctcgg cgttgatgcg
     2941 gcactcgatc gcgtgaccgc ggatgtgcac gtcgtcctgc gaccactgca gccgctcgcc
     3001 cgcggcgatc ctcagctgcg ccttcacgag gtcgatgccg gtgatgatct ccgtcaccgg
     3061 atgctcgacc tggatgcgcg tgttcatctc gatgaagtag aactcgtcgt cctggtacag
     3121 gaactcgagc gtgccggcgc tgcgataacc gatgtcggcg caggcggcag cgacgcgatc
     3181 gctcatgtgc ttgcgctgct tggccgtgag gccgggggcc ggcgcctcct cgatcacctt
     3241 ctggtgacgg cgctgcatgg agcagtcgcg ctcgccgagg cagatcacgt tgccgtagtg
     3301 gtcggcgagc acctggaact cgatgtgccg cggccgctcc aggaacttct ccatgtacac
     3361 ctggtcgttg ccgaacgccg caagggcctc ggcgcgcgtc acgccgacgg agtggatgag
     3421 cgtcgcctcg ctgtgcacga cgcgcatccc gcggcccccg ccgccgccgg acgccttgat
     3481 gatcaccggg aagccgatgt cgcgcgcgac gcggatgatc tcgtcgggct cggtgccgag
     3541 cgggccgtcc gagccgggca cgcagggcac gccggccttc ttcatcgtct ggatggccga
     3601 gaccttgtcg cccatcaggc ggatcgtctc ggcgcgcggg ccgatgaagg tgaagccgct
     3661 cttctccacg cgttccgcga agtcggcatt ttccgagagg aagccgtagc cggggtggat
     3721 cccgacggcg tccgtgacct cggcggcgct gatgatcgcc ggcatgttga ggtagctctc
     3781 gcgcgacggc ggcgggccga tgcacaccgt ctcgtccgcg agcagcacgt gcttcaggtt
     3841 gcgatcagcc gtcgaatgca ctgccacggt cttgatcccg agttcccggc acgcgcgcag
     3901 gatgcggagc gcgatctcgc cccggttggc aatgacgacc ttgtcgagca ttactcgatc
     3961 acgaacagcg gctggccgaa ctcgaccggg tcgccgttct tgaccaggat ggacacgacc
     4021 ttgccggcct tgtcgcactc gatctggttc atcatcttca tggcctcgat gatgcacagc
     4081 acgtcgcccg g
//
LOCUS       DAOMFD010000173         4076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9861_length_4076_cov_32.582691, whole genome shotgun sequence.
ACCESSION   DAOMFD010000173 DAOMFD010000000
VERSION     DAOMFD010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9861_length_4076_cov_32.582691"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1153
                     /locus_tag="PL152_08970"
     CDS             <1..1153
                     /locus_tag="PL152_08970"
                     /inference="COORDINATES: protein motif:HMM:NF023282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MtrB/PioB family outer membrane beta-barrel
                     protein"
                     /protein_id="HQR49457.1"
                     /translation="ALPMSNLHGDVDVTRLDLTLTSRPMDKLRLRGSLTYDESKDTSK
                     QAAFDSVVYTDMIPILGSETNPRYGFERFRALGSADWELNSQWTIGAGGEYRELKRTG
                     TPQEVKKDEVLDGWGKVSYRPSGYLGLVLRAGAQETSPDSYNKTIAADNGQNPYMRLY
                     NMAYRFRQYGELTANLALGNLPLTLSGTAYYADDGYNKSQIGLVSGLYQRYAVDLNWA
                     INDKYTAYINAGWDTNDTEQWGSSTYSYRDWKGTVNDDFTTIGLGLNAKLLDKLSLDL
                     SYTYASGNSDTTISGVNAGKFPTVSSDLNSVKADLSYAWNERTDVIFTWWYENLDSRD
                     WALNGIGPATLPNILALGADPYNYSVNYVTLSVRYAFGSEKAEEKAKEE"
     gene            complement(1235..1546)
                     /locus_tag="PL152_08975"
     CDS             complement(1235..1546)
                     /locus_tag="PL152_08975"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR49458.1"
                     /translation="MNKLTLSALLAALALAATPTFAADAAAGKKIAEADCADCHGDDG
                     KGDDETPALAGMATDKFTKAMTEYQNGTRTKDAKMAKAAKKLTAAQVADVAAYYGSLK
                     K"
     gene            complement(1628..3052)
                     /locus_tag="PL152_08980"
     CDS             complement(1628..3052)
                     /locus_tag="PL152_08980"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HQR49459.1"
                     /translation="MRLVFGASGYIGSNLVPALLAEGHAVRATARNVEVLEARGWRGA
                     ELAAADALDPATLPGALRGVDTAYYLVHSMAAGRDFAAIDRRAAENFARAAATAGVRR
                     IVYLGGLIPPNPRSQHLRSRAETGEVLRRFPVPVTEIRAGMIIGPGSAAYEVIRDLVN
                     HLPLMVTPSWVRSRSTPIALADLLAYLVAVPGLAEAAGRIYDVGGPDAVTYEDIMRCY
                     GRLVGRKVRILAVPVLTPRLSSYWLRFVTSVPTPVARALVEGLEHDFVADAAEIRRLV
                     PRRLMGLEGSIRAAIDADMRHAVLIRWVEGAIACRNFRPEYGYYAMRVGAEASGPASP
                     DAVFRTVCTIGGERGYFFANTLWAIRRAADWLVGGPSFRRRRRHPTELRVGDVIDSWR
                     VIALEPGRRLTLLMEMKGPGAGVLEFTVGPGPDGAGSAVSATAYWHPAGVPGLVYWYG
                     LGPAHAFLFQGLTRTIQRLASKSA"
     gene            complement(3056..3679)
                     /locus_tag="PL152_08985"
     CDS             complement(3056..3679)
                     /locus_tag="PL152_08985"
                     /inference="COORDINATES: protein motif:HMM:NF013935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HQR49460.1"
                     /translation="MPDAGGEVLATRVAFLELQDERRLVQEGYELLDEKRMLLATEIM
                     RQLRKHRELKEILAVHEREAREALEAAVDACGFDDLTVHPKRSLEDSLLRTNAQHFLG
                     ITLQDAALDAPVQGADPPSPTPFPEARACGWAFRGLVADHAAVAACAGNLRRLVREYT
                     RTERRARALENVVIPEIDVSLRYIEEQLDALDQEEAVRVRNAAGARS"
     gene            complement(3672..>4076)
                     /locus_tag="PL152_08990"
     CDS             complement(3672..>4076)
                     /locus_tag="PL152_08990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49461.1"
                     /translation="GQIVLDRELDRRGVYPPIRVLPSLSRLMDAGTGEGYTHADHPAL
                     AHQLFAAYARAVRVRMLASVMGEQGLPDADKSFLAFGNRFENDLVRQAGPRTLEESME
                     AGWKALRGLPRTELTRLSNDQIKRHLEDGGDA"
BASE COUNT          649 a         1415 c         1427 g          585 t
ORIGIN      
        1 cgcgctgccg atgagcaacc tgcacggcga cgtggacgtc acgcgcctcg acctcacgct
       61 cacctcgcgt cccatggaca agctgcgcct gcgcggctcg ctcacctacg acgagagcaa
      121 ggacacgagc aagcaggctg cgttcgactc cgtggtgtac acggacatga tcccgatcct
      181 cggcagcgag accaacccgc gctacggatt cgagcgcttc cgcgcgctcg gcagcgcgga
      241 ctgggaattg aacagccagt ggacgatcgg cgccggcggc gagtaccgcg agctcaagcg
      301 cacgggcacg ccccaggaag tgaagaagga cgaggtgctc gacggctggg gcaaggtctc
      361 gtaccggccg agcggctacc tgggtctggt gctgcgcgcc ggcgcgcagg agacgagccc
      421 cgacagctac aacaagacga tcgccgcgga caacggccag aacccgtaca tgcgcctgta
      481 caacatggcc taccggttcc ggcagtacgg cgaactcacg gccaacctcg cgctcggcaa
      541 cctgccgctg acgttgagcg gcacggccta ctacgcggac gacggctaca acaagtcgca
      601 gatcgggctc gtatcgggtc tctaccagcg ctacgcggtg gacctgaact gggcgatcaa
      661 cgacaagtac accgcctaca tcaatgccgg ttgggacacg aacgacacgg agcagtgggg
      721 cagcagcacg tactcctacc gcgactggaa gggcacggtg aacgacgact tcacgacgat
      781 cggtctcggc ttgaacgcga agctgctcga caagctgtcg ctcgacctgt cgtacaccta
      841 cgcgtcgggc aactccgaca cgaccatctc gggtgtcaat gccgggaagt tcccgaccgt
      901 gagcagcgac ctgaacagcg tcaaggcgga cctaagctac gcgtggaacg agcgcaccga
      961 cgtgatcttc acgtggtggt acgaaaacct ggactcgcgc gactgggcgc tgaacggcat
     1021 cggcccggcg acgctgccga acatcctggc gctgggcgcc gacccgtaca actattccgt
     1081 gaactacgtg acgctgtcgg tccgttacgc gttcgggtcg gagaaggccg aagagaaggc
     1141 caaggaagaa tgaggtgggg gaaaggaccg gcgggctgcg gcatggccgc agccgccggg
     1201 tccagggccg gcgcgtgccg gccccggcag cgaattactt cttgaggctg ccgtagtacg
     1261 cggcgacgtc cgcgacctgg gccgccgtga gcttcttggc cgccttggcc atcttggcgt
     1321 ccttggtgcg cgtgccgttc tggtactccg tcatggcctt ggtgaacttg tccgtcgcca
     1381 tgccggccag cgccggcgtc tcgtcgtcac ccttgccgtc gtcgccgtgg cagtcggcgc
     1441 agtcggcctc ggcaatcttc ttgcccgcag ccgcgtccgc cgcgaaggtc ggcgtggcgg
     1501 ccagcgcgag ggcggcgagc agggcggaca aggtcagctt gttcatctcg agatctcctg
     1561 gaagtgattc ggtcccgacg gtgttagacc gcggagcgcg gattttgctc ccaagccgcg
     1621 tctcccgcta ggcgctcttg ctcgcgagac gctggatcgt gcgggtcagc ccctggaaca
     1681 ggaacgcgtg cgctggaccg agtccatacc agtacacgag cccgggcacg ccggcggggt
     1741 gccagtaggc agtggcgctg acggccgacc cggcaccgtc cgggcctggc ccgacggtga
     1801 actcgagcac ccccgcgccg gggcccttca tctccatcag cagtgtcagg cgccgcccgg
     1861 gctcgagcgc gatcacgcgc cacgagtcga tgacgtcgcc gaccctgagc tcggtcgggt
     1921 gccggcgacg gcggcggaag ctcggtccgc cgacgagcca gtctgcggcg cgccggatcg
     1981 cccacagcgt gttggcgaag aagtaccccc gctcgccgcc gatcgtgcac accgtcctga
     2041 acacggcgtc cgggctcgcc gggcccgagg cctccgcccc gacccgcatc gcgtagtagc
     2101 cgtactcggg acgaaagttg cggcacgcga tggcgccctc gacccagcgt atgaggaccg
     2161 cgtgccgcat gtcggcgtcg atggcggcgc ggatggaccc ctcgagtccc atgagccggc
     2221 gggggaccag ccggcggatc tccgcggcgt cggcgacgaa atcgtgctcg agcccctcga
     2281 cgagcgcacg cgcgacgggg gtcgggaccg aggtgacgaa tcgcagccag tacgacgaga
     2341 ggcggggcgt cagcacgggg acggccagga tgcgcacctt gcggccgacg aggcgaccgt
     2401 agcagcgcat gatgtcctcg taggtcaccg catcgggacc gcccacgtcg tagatgcggc
     2461 ccgcggcctc cgcgagtccg gggacggcga ccaggtacgc caggaggtcg gccagcgcga
     2521 tcggggtcga gcgcgaccgc acccacgacg gcgtgaccat cagcgggagg tggttgacta
     2581 ggtcccggat gacttcgtag gccgccgatc ccgggccgat gatcatgccc gcccgaatct
     2641 cggtgaccgg caccgggaac cgccggagca cctcgccggt ctcggcccgc gagcgcaggt
     2701 gttgcgagcg cgggttcggc gggatgagcc ccccgagata cacgatgcgg cgcacgccgg
     2761 ccgtggcggc ggcccgggcg aagttctcgg ccgcccggcg gtcgatcgcg gcgaagtcgc
     2821 ggccggccgc catcgagtgc acgaggtagt aggccgtgtc cacgccccgg agcgcgccgg
     2881 gcagcgtggc gggatcgagg gcgtccgcgg cggcgagttc cgcgccgcgc cagccacggg
     2941 cctcgagcac ttcgacgttg cgcgcggtcg cgcgtacggc gtgcccctcg gcgagcagtg
     3001 ccgggaccag gttgctgccg atgtagccgc tggctccgaa gacgaggcgc aaggctcagg
     3061 acctcgcacc ggccgcattg cggacccgca ccgcctcttc ctggtccagg gcgtccagct
     3121 gctcctcgat gtagcgcagc gacacgtcga tctccgggat cacgacgttt tccagcgccc
     3181 gcgcccggcg ctcggtacgg gtgtattcgc gcaccaggcg gcgcaggttg cccgcacagg
     3241 ccgcgaccgc agcatggtcg gccaccaggc cccggaacgc ccagccgcac gccctggcct
     3301 cgggaaaggg cgtcggcgag ggtgggtccg ccccctggac gggcgcgtcc agcgcggcgt
     3361 cctgcagcgt gatgccgagg aagtgctggg cgttcgtccg cagcagcgag tcctccaggc
     3421 tgcgcttggg atgcaccgtc aggtcgtcga agccgcaggc gtcgaccgcg gcctcgagtg
     3481 cctcgcgggc ctcccgctcg tggacggcca ggatttcctt caattcacgg tgctttcgca
     3541 gctgccgcat gatctcggtc gcgagcagca tgcgcttctc gtcgaggagc tcgtagcctt
     3601 cctggacgag gcgccgctcg tcctgcagct cgaggaacgc gacgcgggtc gcgaggacct
     3661 ctccgcccgc gtcaggcatc gccgccgtcc tcgaggtggc gcttgatctg gtcgttcgac
     3721 aggcgcgtga gttccgtgcg cggcaggccg cgcagcgcct tccacccggc ctccatgctt
     3781 tcctccagcg tgcgcgggcc ggcctggcgc accaggtcgt tctcgaagcg attgccgaac
     3841 gcgaggaagc tcttgtcggc atccggcaat ccctgctcac ccatcacgct cgccagcatc
     3901 cgcacgcgca ccgcgcgcgc ataggccgcg aacagctggt gggcgagggc cggatggtcc
     3961 gcgtgcgtgt aaccctcgcc ggtgcccgca tccatcagcc gcgacaggct cggcagcacg
     4021 cggatcggcg gatagacgcc gcggcggtcg agctcgcggt cgagcacgat ctggcc
//
LOCUS       DAOMFD010000174         4075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9868_length_4075_cov_26.564179, whole genome shotgun sequence.
ACCESSION   DAOMFD010000174 DAOMFD010000000
VERSION     DAOMFD010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9868_length_4075_cov_26.564179"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(148..1524)
                     /locus_tag="PL152_08995"
     CDS             complement(148..1524)
                     /locus_tag="PL152_08995"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR49462.1"
                     /translation="MSVSQVAATVSRLRHTFDSGKTRPIEWRLAQLREIERALHEHEA
                     DFTEALRKDLAKPSFESGMAEMGWAKAEAKYARKHLGNWMKPTRVRTPLMAQPGRSYI
                     EPEPKGVVLIIAPWNYPLSMIMCPLVGAVAAGNCAVMKPSEITSHTSAALASILPRYL
                     DQEAFAVVEGGIPETTELLEQKFDHILYTGNERVATVVMTAAAKHLTPVTLELGGKSP
                     CLIDKSADIEVAASRVAWGKWVNAGQTCVAPDHVLVHRDVAQPFIDAITRRLKEFYGE
                     DPAQSPDFGRIASERHTARFAELLKGHKIHVGGRVDVARRYVEPTIVLDPPADSALMT
                     EEIFGPVLPVITVDEMHHAIKFVADRPKPLALYVFTKSKALEEAVLGRISAGSVCIND
                     AVIFMVSPELPFGGVGNSGMGRYTGWYGFETFSHMRPVMKRSYRFDMPLRYPPYTEQK
                     AKLIRKVM"
     gene            1690..2208
                     /locus_tag="PL152_09000"
     CDS             1690..2208
                     /locus_tag="PL152_09000"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HQR49463.1"
                     /translation="MADEVRNVFKGRVLTLNLEKVTLPNGRVAELEIAHHPGGAAVVA
                     LDDAGRVCLLRQYRHAAGGWVREIPAGKLDGGEPPLRCAQRELAEEAGVRAARWDKLG
                     EYLSSPGVLTEVIHVYLARELSPVGAAPEDHEVLEVEWLPLRDALQLAASGELRDGKS
                     LVGLFWTGAAVS"
     assembly_gap    2281..2380
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2381..2831
                     /locus_tag="PL152_09005"
     CDS             <2381..2831
                     /locus_tag="PL152_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49464.1"
                     /translation="GGGFCNTARVDPPRQRSSTMAEKPGSKAPPTPPEQSRDSSDAGR
                     LGKDDRGNVTWEWKEEGDLLADDVVGDAERVRALVDPKLEIVEDEEGPDNDPTSVRLE
                     KLKKGYNPYSSGPLGKQSWKKKKDLRELSEWIELRKKMAEKKKDDGG"
     gene            2873..3379
                     /gene="mobB"
                     /locus_tag="PL152_09010"
     CDS             2873..3379
                     /gene="mobB"
                     /locus_tag="PL152_09010"
                     /inference="COORDINATES: protein motif:HMM:NF015181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HQR49465.1"
                     /translation="MRVYGIVGRKNAGKTHLVVRLVTAAVARGLVVSTVKHAHHAFDV
                     DQPGKDSHRHREAGAREVIVSSALRFALVHEHRGGAEPGLAELLARLSPCDLVLVEGF
                     KASDHPKLEVYRAACGQPPLAAEGAAVEAIALDAGSRVETSLPRLPLDDTEAVLDFIL
                     GRATTRAP"
     gene            complement(3345..4034)
                     /locus_tag="PL152_09015"
     CDS             complement(3345..4034)
                     /locus_tag="PL152_09015"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HQR49466.1"
                     /translation="MRDVTAVIFDLDDTLWDVGPVILRAEQAMARFLAERYPRVMELN
                     SLESMRDLRARMALEHPAMRHDFTWLRTRALLEHAREAGYPEAMAREAFDVFFRARNE
                     VVLYDDVVPTLERLRGRFRLFAISNGNADLAAIGLAGHFERSLAAREAGVLKPDPRIF
                     EMLLDAASIAPAHTVHVGDDPEADVEGARRAGIRPIWVNRHGSRWPAHLPAPAHSVAT
                     LTELAWLLDPG"
BASE COUNT          642 a         1350 c         1389 g          594 t
ORIGIN      
        1 cgatccttgg cctcgtgctc ggcctcggca tgtcgtgggc gttgatccgc cagaaagcct
       61 cgggacaggc gtccgtggac cgcgtcgaca cgtagggagg ggacattctc ctatttcggg
      121 acgaaatagg agaatgtccc caattcccta catcaccttc cggatcagct tggccttctg
      181 ttcggtgtag ggcggatagc gcagcggcat gtcgaagcgg tagctgcgct tcatcaccgg
      241 ccgcatgtgg ctgaaggtct cgaagccgta ccagccggtg tagcgaccca tgccgctgtt
      301 gccgacgcca ccgaacggca gctccggcga caccatgaag atcaccgcgt cgttgatgca
      361 gacgctgccg gcactgatgc gcccgagcac cgcctcctcg agggccttgc tcttcgtgaa
      421 cacgtagagc gccagcggct tcgggcgatc cgccacgaac ttgatggcgt ggtgcatctc
      481 gtcgaccgtg atcaccggca ggaccggccc gaagatctcc tcggtcatca acgccgagtc
      541 cgccggcggg tccagcacga tggtcggctc gacgtagcgc cgggccacgt ccacccgccc
      601 gccgacgtgg atcttgtgtc ccttgagcag ctcggcgaag cgcgccgtgt gccgttcact
      661 cgcgatccgc ccgaagtccg ggctctgcgc cgggtcctcg ccgtagaact ccttgagccg
      721 gcgcgtgatc gcgtcgatga acggctgcgc cacgtcgcga tggaccagca cgtggtcggg
      781 cgcgacgcag gtctggccgg cgttcaccca cttgccccag gcgacacggg aggccgccac
      841 ctcgatgtcg gcgctcttgt cgatcaggca cgggctcttg ccgcccagct ccagcgtcac
      901 gggcgtcagg tgtttcgccg cggccgtcat gacgaccgtc gccacgcgct cgttccccgt
      961 gtagaggatg tggtcgaact tctgctcgag cagttccgtc gtctcgggga taccaccttc
     1021 gacgacggcg aaggcttcct ggtcgaggta gcgagggagg atcgacgcca gcgccgcgga
     1081 ggtgtggctc gtgatctccg aaggcttcat gaccgcgcaa ttcccggccg cgacggcgcc
     1141 gaccagcggg cacatgatca tcgacagcgg gtaattccac ggcgcgatga tcaggacgac
     1201 gcccttcggc tccggctcga tgtagctgcg gcccggttgc gccatgaggg gcgtgcgcac
     1261 ccgtgtcggc ttcatccagt tgccaaggtg cttgcgggcg tacttcgcct ccgccttggc
     1321 ccagcccatc tccgccatgc ccgactcgaa gctgggcttg gcgaggtcct tgcgcagcgc
     1381 ctcggtgaag tcggcctcgt gctcgtgcaa ggcgcgctcg atctcgcgga gctgcgcgag
     1441 ccgccactcg atcgggcggg tcttaccgct gtcgaacgtg tggcgaagcc tggacacggt
     1501 ggccgcaacc tgcgaaacgc tcatggaatg ctccgtggcc ccgcatccgg cggggcgagg
     1561 ctcgcgacgt tagcacagct ggccgcaggg tggtgcggcg caacatgcgg ggcccaggcg
     1621 gggcccaggc cgggcccacg gcaagcgcct gtatcatcgg cgaccggcac ggcagtgact
     1681 ggaacgtaca tggccgacga agtccgcaac gtattcaagg gccgcgtgct gacgttgaat
     1741 ctcgagaagg tgacgctccc caacggccgc gtcgccgagc tcgagatcgc gcatcacccg
     1801 ggcggtgccg ccgtggtggc gctcgacgac gcgggccggg tctgcctgct gcgccagtac
     1861 cgccacgcgg cgggcggctg ggtgcgggag attcctgccg gaaagctcga cggcggcgag
     1921 ccgccactgc gatgcgccca gcgcgaactg gctgaggaag ccggcgtccg ggcggcgcgg
     1981 tgggacaagc tcggcgaata cctgagttcg ccgggcgtcc tgacggaagt catccacgtg
     2041 tacctggcgc gcgagctgtc accggtgggc gcggcaccgg aggaccacga ggtcctcgag
     2101 gtcgagtggt tgcccttgcg cgacgccctg cagcttgcag ccagcggtga gctgcgggac
     2161 ggcaagagcc tggtgggcct gttctggacc ggcgcggcag tttcgtgacg gagcggtatt
     2221 tcgcggggtg ggggattctg caacactgcg cgcgtcgatc ccccgcgcca gcggagcagc
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn gggtggggga ttctgcaaca
     2401 ctgcgcgcgt cgatcccccg cgccagcgga gcagcaccat ggccgagaag cccggctcga
     2461 aagcgccccc cacccccccg gagcagtcca gggacagtag cgatgcaggt cgcctcggca
     2521 aggacgaccg gggcaacgtg acgtgggaat ggaaggagga aggcgacctg ctcgcggatg
     2581 acgtggtcgg cgacgccgag cgcgtgcgcg ccctggtcga ccccaagcta gagatcgtgg
     2641 aagacgaaga ggggccggac aacgacccga cgtcggtccg gctcgagaag ctgaagaagg
     2701 gctacaaccc gtacagcagc ggcccgctcg gcaagcagag ctggaagaag aagaaggacc
     2761 tccgcgagct gtccgagtgg atcgagttgc gcaagaagat ggccgaaaag aagaaggacg
     2821 acggcggcta gcccacggcg tcccggcgca ccgcagcagc aggacacccg ccatgcgcgt
     2881 ctacggcatc gtcggccgca agaacgcggg caagacccac ctcgtcgtgc gtctcgtcac
     2941 cgcagctgtg gcccgggggc tcgtcgtctc gaccgtgaag cacgcgcacc acgccttcga
     3001 cgtcgaccag ccgggcaagg acagccacag gcatcgcgag gccggcgcgc gcgaggtcat
     3061 cgtgagctcc gcgctgcgct tcgcgctcgt gcacgagcat cgcggcggcg cggaacccgg
     3121 cctcgcggag ctgctcgcgc ggctgtcgcc ctgcgacctc gtgctggtcg agggattcaa
     3181 ggccagcgac catcccaagc tcgaggtcta ccgggccgcc tgcggtcaac cgccgttggc
     3241 ggccgaagga gccgcggtag aggccatcgc gctcgacgcc ggaagccgcg tggagacgag
     3301 cctgccgcga ctgccgctgg acgacaccga ggccgtgctc gacttcatcc tgggtcgagc
     3361 aaccacgcga gctccgtaag cgtcgcgacc gagtgcgccg gggccggcag gtgcgccggc
     3421 caccgcgatc cgtgcctgtt cacccagatc ggccggatgc ccgcgcgacg ggcgccctcg
     3481 acgtcggctt cgggatcgtc cccgacgtgc acggtgtgtg cgggcgcaat actggctgcg
     3541 tcgagcagca tctcgaagat ccgcgggtcc ggcttcagca cgcccgcctc gcgcgcggcg
     3601 aggctgcgct cgaaatgccc tgcaagcccg atcgcggcga ggtccgcgtt gccgttgctg
     3661 atcgcgaaca gccggaacct gccgcgcagc cgctcgaggg tgggcaccac gtcgtcgtac
     3721 agcacgacct cgttgcgcgc acgaaagaag acgtcgaacg cctcgcgggc catcgcctcc
     3781 gggtatcccg cctcccgcgc gtgctccagc agcgcgcgag tgcgcagcca cgtgaagtcg
     3841 tgccgcatcg ccgggtgctc cagcgccatg cgcgcgcgca ggtcgcgcat cgactccagc
     3901 gagttaagtt ccatgacgcg cgggtagcgc tccgcgagga acctcgccat cgcctgctcg
     3961 gcgcgcagta tcacgggacc gacgtcccac agcgtgtcgt cgaggtcgaa gatgacggca
     4021 gtgacgtcgc gcatccgtgc tcagcgccgc gtgcgctcgg cgaggaagga cttga
//
LOCUS       DAOMFD010000175         4047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_9978_length_4047_cov_29.926353, whole genome shotgun sequence.
ACCESSION   DAOMFD010000175 DAOMFD010000000
VERSION     DAOMFD010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4047
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9978_length_4047_cov_29.926353"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="PL152_09020"
     CDS             complement(<1..317)
                     /locus_tag="PL152_09020"
                     /inference="COORDINATES: protein motif:HMM:NF015019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HQR49467.1"
                     /translation="MDTNSALMLAIGAGIAAILYGVFSVRWILAQPAGNARMQEIAAA
                     VQQGASAYLNRQYTTIAMVGVVLFVILGVALGWHTAGGFAVGAILSGAAGYIGMNVSV
                     RSNV"
     gene            complement(408..1682)
                     /locus_tag="PL152_09025"
     CDS             complement(408..1682)
                     /locus_tag="PL152_09025"
                     /inference="COORDINATES: protein motif:HMM:NF010675.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HQR49468.1"
                     /translation="MKNKSPARNAFYAQSGGVTAVINASACGVIETSRVHKTRIRNVY
                     AGRHGIIGALTEDLIDTSQESAAAIRALRHTPSGAFGSARYKLKKYEDNPAQYERLIE
                     VFKAHDIGYFFYNGGGDSADTCLKVSMLAEKLGYPLTAIHIPKTVDNDLPITDCSPGF
                     GSVAKYVAVSIREAGFDVASMARTSTKVFVLEVMGRHAGWIAAAGGLAAEKAGDAPHI
                     ILFPEITLDEDRFLRRVKECVDKYGYCAIVVSEGVKNAEGKFLADAGTRDAFGHAQLG
                     GVGPVVAQLVKDKLGYKYHWAVADYLQRAARHIASKVDVEQAYAVGKAAVELAVKGHH
                     AVMPAIVRTSNKPYRWKIGVAPLDRVANVEKMLPRDYITPDGFHITTKGRRYLAPLIR
                     GEDYPPYKDGLPQYVVLKNRPVRRKLKTTFVV"
     gene            1898..2554
                     /locus_tag="PL152_09030"
     CDS             1898..2554
                     /locus_tag="PL152_09030"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HQR49469.1"
                     /translation="MRLVLLGAPGSGKGTQAQRLRDDRGIPQVSSGDLLRDAVARGTE
                     LGKRAKAAMDAGELVSDEIVLGLIRERLGRPDAAKGFILDGYPRNVAQAGALEKLLAE
                     IGQPIDAVVLMDVDPKALFKRLTGRRSCPKCSKVFNVYSSPSANGDRCDNHAGEEAPL
                     LVQRADDKEDVIANRLKVYESQTQPLVEHYKALRLLRVVNADQPVEKVYAEFLRAAGG
                     "
     gene            complement(2606..3709)
                     /gene="rnd"
                     /locus_tag="PL152_09035"
     CDS             complement(2606..3709)
                     /gene="rnd"
                     /locus_tag="PL152_09035"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HQR49470.1"
                     /translation="MRDATLVTTQSQLDAAVFRLQPASRLAIDTEFMRERTYFPQLCL
                     LQVATDADCFLVDPLAGLDLDALHALLADRSKLKILHAARQDLEVLQLGGRAVPAPVF
                     DTQVAAAMLGFPPQAGYAELVARQLGHSIDKGQTRTDWSRRPLTPEQLAYAADDVHHL
                     LTLHAELAAALAARGRAAWVAEDATALEDPALYRTEPVDAWRRLKGLGRLKPREQAAA
                     RALAEWRETRAIEADKPRGWILADEALYAIAMGLPRTASELGSIPAMPPATVRKRGEE
                     LLALIAAALEAPVAPGGPAGSGKPTPQENALVARLQQLVRDQATALGVSPEVIATRRD
                     VEALVFKDRGESAVLRGWRREVIGDKLLAALEK"
     gene            3767..>4047
                     /locus_tag="PL152_09040"
     CDS             3767..>4047
                     /locus_tag="PL152_09040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49471.1"
                     /translation="MQQFLRIFLDIVLWQRGPQDLPASPLLVWITLAAYVVVGAVQLA
                     LLDESGPVWFVFLVMDPALLLATVWLMLRLYGRTGRFQQTAAAVLGTGAL"
BASE COUNT          618 a         1348 c         1389 g          692 t
ORIGIN      
        1 acgttggagc gcaccgacac gttcatgccg atgtagcccg cggcgcccga gaggattgcg
       61 ccgaccgcga aaccgcccgc cgtgtgccag ccgagcgcca cgccaaggat cacgaacagc
      121 acgaccccga ccatggcgat cgtcgtgtac tgccggttga gataggcgct cgcgccctgc
      181 tggaccgccg cggcgatctc ctgcatgcgg gcattgccgg ccggctgggc caggatccag
      241 cgcacggaga acactccgta caggatcgcg gcgatgcctg ccccgatcgc gagcatgagc
      301 gcggaattgg tatccatctg caaacctccc aacgagggtt tccgggttcg attcagcggc
      361 ccgccgggct ctggcgtccc ttgacgcgcc gcaaaaaagg cgcgcgacta taccacaaag
      421 gtggtcttga gcttcctccg caccgggcgg ttcttgagga cgacgtactg cggcaggccg
      481 tccttgtagg gcggatagtc ctcgccccgg atgaggggag ccaggtagcg ccggcctttc
      541 gtggtgatgt ggaatccgtc gggggtgatg tagtcccggg gcagcatctt ctcgacgttg
      601 gcgacccggt ccaggggggc gacgccgatc ttccagcgat acggcttgtt ggacgtgcgg
      661 acgatcgccg gcatcactgc gtggtgaccc ttgaccgcca gttccaccgc ggccttgccg
      721 acggcgtagg cctgttcgac gtcgaccttc gaggcgatgt gccgggccgc cctctgcagg
      781 tagtcggcca cggcccagtg gtacttgtag ccgagcttgt ccttgacgag ttgcgcgacc
      841 accgggccga cgccgccgag ctgggcgtgc ccgaatgcat cacgcgttcc ggcatcggcg
      901 aggaacttgc cctcggcgtt cttcacgcct tcggagacga cgatggcgca gtagccgtac
      961 ttgtccacgc attccttgac gcggcggagg aaccggtcct cgtcgagggt gatctccggg
     1021 aacaggatga tgtgtggtgc gtcgccggct ttctccgcgg cgagcccgcc cgccgcggca
     1081 atccagcccg cgtggcggcc catgacctcg agcacgaaga ccttcgtgct cgtgcgcgcc
     1141 atcgaggcga cgtcgaagcc cgcctcgcgg atcgacacgg ccacgtactt ggccaccgaa
     1201 ccgaagcccg gcgagcagtc ggtgatcggc aggtcgttgt ccaccgtctt ggggatgtgg
     1261 atcgccgtga gcgggtagcc gagcttctcc gccagcatcg acaccttgag gcaggtgtcc
     1321 gccgagtcgc cgccgccgtt gtagaagaag tagccgatgt cgtgcgcctt gaagacctcg
     1381 atcaggcgct cgtactgcgc cgggttgtcc tcgtatttct tgagcttgta tcgggccgac
     1441 ccgaatgcac ccgacggggt atgtcgcagt gcccggatgg cggcggcact ttcctggctc
     1501 gtgtcgatca ggtcctcggt cagtgcgccg atgatgccgt gccgtcccgc gtagacgttt
     1561 ctaatccggg tcttgtgcac ccggctggtc tcgatcacgc cgcaggccga ggcgttgatg
     1621 acggcggtga ccccgcccga ctgtgcgtag aaggcattgc gcgcggggct cttgttcttc
     1681 atgtcggctc caaaaaaacg tatggatatc gtggacttat ggtccgtgga gtaccgtcta
     1741 gcatacccga agccgcgtcc gtcccgcgtc agggaaaatg gggacattca ccaattcgca
     1801 gtcgcgaatt ggtgtatgtc cccattttcc caattggcgc tggcgacgct tttcggtagg
     1861 gtgccgcctc ccgactcggc gcgtggacga ccataagatg agactcgtac tgctcggagc
     1921 acctggatca ggaaagggca cgcaggctca gcgcctgcgc gacgaccgcg gcattcccca
     1981 agtgtcgtcc ggggacctgc tgcgcgacgc ggtggcccgc ggcaccgagc tgggcaagcg
     2041 cgccaaggcc gcaatggacg ccggcgagct cgtgtccgac gagatcgtgc tgggactcat
     2101 acgcgagcgg ctcggccgac cggacgcggc gaaaggtttc atcctcgacg ggtacccgag
     2161 gaacgtcgcg caggcgggcg cgctcgagaa gctgctggcc gagatcgggc agccgatcga
     2221 cgccgtcgta ctgatggacg tcgacccgaa ggccctgttc aagcgcctga cgggccggcg
     2281 cagctgcccg aagtgcagca aggtgttcaa tgtctacagc tcgccgtccg ccaacggtga
     2341 tcgctgcgac aaccacgccg gcgaggaagc gccgctgctg gtgcagcgcg ccgacgacaa
     2401 ggaggacgtc atcgccaacc ggctcaaggt gtacgagagc cagacgcaac cgctggtgga
     2461 acactacaag gcgctgcggc tgctccgcgt cgtgaacgcc gaccagccgg tcgagaaggt
     2521 gtacgcggag ttcctgcgcg ccgcgggcgg ctgaagaata ggggacattc tcctatttcg
     2581 gtcgaccgaa ataggagaat gtcccctatt tctcgagggc ggcgagcagc ttgtcgccga
     2641 tcacctcgcg ccgccagccg cgcagcaccg ccgactcgcc gcggtccttg aacaccagcg
     2701 cttcgacatc gcgccgcgtg gcgatcacct ccgggctgac cccgagcgcg gtcgcttggt
     2761 cgcggacgag ttgctgcagt cttgcgacga gcgcgttctc ctgcggggtc ggtttcccgc
     2821 tccctgccgg gccgcccggc gcgaccgggg cctccagcgc tgcggcgatg agcgccagca
     2881 gttcctcgcc gcgcttgcgc accgtcgcgg gcggcatcgc cggaatcgat cccagctcgg
     2941 acgcggtccg cggcaggccc atggcgattg cgtagagcgc ttcgtccgcc agtatccaac
     3001 cccgcggctt gtccgcctcg atcgcgcggg tctcacgcca ctcggccagc gcgcgcgcgg
     3061 cggcctgttc ccgcggcttc aggcgcccaa gtcccttgag gcggcgccac gcgtcgacag
     3121 gctccgtgcg atagagcgcg gggtcctcga gcgccgtggc gtcctcggcg acccatgccg
     3181 cgcgaccgcg cgccgccaac gccgccgcaa gttccgcatg cagcgtcagc aggtggtgca
     3241 cgtcgtcggc cgcatatgcg agttgctcgg gggtgagcgg gcgccgtgac cagtccgtgc
     3301 gggtctggcc cttgtcgatg gagtggccga gctgccgcgc gacgagctcc gcgtatccgg
     3361 cctgcggcgg gaatccgagc atggccgccg cgacctgcgt gtcgaagacc ggggccggga
     3421 cggcgcgacc gccgagttgc agcacctcga ggtcctgccg cgcggcgtgc aggatcttga
     3481 gcttcgagcg gtcggcgagc agcgcatgca gcgcgtcgag gtcgaggccg gccagcggat
     3541 cgacgaggaa gcaatcggcg tcggtcgcca cctgcagcag gcacagctgc gggaaatagg
     3601 tgcgctcgcg catgaattcc gtgtcgatcg cgaggcgcga cgccggctgg agcctgaaca
     3661 cggctgcgtc cagctgtgat tgggtcgtga ccagcgtggc gtcgcgcatt tttcggcggt
     3721 acctccgtgt agaatcgcgg ctccccgggc ccgccgcgac ggcaacgtgc agcagttcct
     3781 caggatcttc ctcgacatcg tactctggca gcgcggcccg caggaccttc ccgcgtctcc
     3841 gctgctcgtg tggatcacgc tcgccgccta cgtcgtggtt ggcgccgtgc agctcgcgct
     3901 gctcgacgag agcgggcccg tgtggttcgt gttcctggtc atggatccgg cgctgctcct
     3961 cgcgaccgta tggttgatgc tgcggctcta tggacggacc ggccggttcc agcagaccgc
     4021 ggcggcggtg ctcggtacgg gcgcact
//
LOCUS       DAOMFD010000176         4024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10076_length_4024_cov_29.260267, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000176 DAOMFD010000000
VERSION     DAOMFD010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4024
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10076_length_4024_cov_29.260267"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /gene="ilvC"
                     /locus_tag="PL152_09045"
     CDS             complement(<1..946)
                     /gene="ilvC"
                     /locus_tag="PL152_09045"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: protein motif:HMM:NF004017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HQR49472.1"
                     /translation="MRAYSKKDVDRKALKGARIAIVGYGSQGRAHALNLKDSGYDVVV
                     GVRKGDSWKKAKKDGLKVMTPVEAVKGATLVALLTPDTTQAENYKEIRANLEPGATLL
                     FAHGFNIHFKQIKPRKDLDVVLIAPKGPGDLVRRQYQQGRGVPCLIAVAQDASGHARA
                     KALAYADGIGGTRAGVLETTFKEETETDLFGEQAVLCGGATELVLKGYETLVEAGYQP
                     EVAYYECLHELKLIVDLLHEGGLAKMHQYISHTAKWGDLTRGPRVVNRDTKKEMKKIL
                     KEVQDGSFARVWIRENRTGRRKYDALMKKDLAHPIEKVG"
     gene            1277..>2208
                     /locus_tag="PL152_09050"
     CDS             1277..>2208
                     /locus_tag="PL152_09050"
                     /inference="COORDINATES: protein motif:HMM:NF013079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49473.1"
                     /translation="MFEEHDTGAARPLWLVDEQGLASWLESQDPPTRQWLTAMRFRAE
                     RHQVACMPADGGGVRMAVLGLGRLPGLESLEPWHLAGAPDRLPAGAWRIATALPAAAA
                     TAAALGWAYGAYRFERYRARSSAAAGAAALVHPQLADMTHVRRMAGALAMARDLINTP
                     ASDLTPASLADQALELAVRCGGHGHVVSGDALREGFPAIHAVGRAADTAPRLVDFSWG
                     DPAAPRVTLVGKGVCFDSGGLDIKGSAGMLLMKKDMGGAACVLALARVIMESALPVRL
                     RVLIPAVENSIAGNAYRPGDVIRTRKGLAVEVGNT"
     assembly_gap    2209..2308
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2309..2517
                     /locus_tag="PL152_09055"
     CDS             <2309..2517
                     /locus_tag="PL152_09055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49474.1"
                     /translation="GDLNNVSASPFAGAIIGGLFLRRFVTQAKDWLHVDLYAWNGKDR
                     PGRPVGAEPQAVRALYALLVDRYG"
     gene            2527..3786
                     /locus_tag="PL152_09060"
     CDS             2527..3786
                     /locus_tag="PL152_09060"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR49475.1"
                     /translation="MNLRYERRAWTLIYLCLGMIEGGTAAVAVRSLFGATVPDLAVDL
                     VVALVSSAPAWSNLASLAYARRAQGRPKVAFLQPLLFAVAGCVAVLSLVPRGGAGLVV
                     FLVAYAASRLLWAGVDTVRSVIWSVNYPRHLRASVAGRIMVASSLALAGTGLLLGWLL
                     GRGGPWFRFAIAGAACCGLAGAAAFSRFRVRREVSLLAAERARLDEGARFDLAGIRDV
                     LARDAGFRHYMYAMSLFGAGVLSLPPLMIICLGDVLHLSAFVQVAVTSSLPILVMPVA
                     LQPWARFLDRHHVVVYRSVHGLVGTAATLLLVSAVLFRAPLLLWPGAVLFGTSLAAGS
                     LGWNLGHNDFAPRGEETRYMALHVTLTGVRGLLMPPLAVALYHALDAWRPGTGSWALL
                     LPLLLVAAGAREFDAMRRLGDIRRPPA"
     gene            3840..>4024
                     /locus_tag="PL152_09065"
     CDS             3840..>4024
                     /locus_tag="PL152_09065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49476.1"
                     /translation="MTRRKADPRAYSRLVYDGVKQTPSRAMLRAVGYTDRDFRRPQVG
                     IASTWSNLTPCNMHIDKL"
BASE COUNT          531 a         1334 c         1362 g          697 t
ORIGIN      
        1 ggccgacctt ctcgatcggg tgcgcgaggt ccttcttcat cagcgcgtcg tacttgcgac
       61 ggccggtccg gttctcgcgg atccagaccc gcgcgaacga gccgtcctgc acttccttga
      121 ggatcttctt catctccttc ttggtgtcgc ggttcacgac ccgcgggccg cgcgtgaggt
      181 caccccactt ggcggtgtgg gagatgtact ggtgcatctt ggcgagcccg ccctcgtgca
      241 gcaggtccac gatcagcttc agctcgtgca ggcactcgta ataggcgacc tcgggctggt
      301 agccggcctc gaccagggtc tcgtagccct tgagcacgag ttcggtggcg ccgccacaga
      361 gcaccgcctg ctcgccgaac aggtcggtct cggtttcttc cttgaaggtc gtctcgagca
      421 cgccggcgcg ggtgccgccg atgccgtccg cataggcgag cgccttggcc ctggcgtggc
      481 cggaggcatc ctgggcgacc gcgatcaggc acggcacgcc gcggccctgc tggtactggc
      541 gacgcacgag gtcaccgggg cccttgggcg cgatcagcac gacgtcgagg tccttgcgcg
      601 gcttgatctg cttgaaatgg atgttgaacc cgtgggcgaa gagcagggtg gcgcccggct
      661 cgaggttcgc gcggatctcc ttgtagttct ccgcctgggt cgtgtccggc gtgagcagcg
      721 caaccagggt ggcgcccttg accgcctcga ccggtgtcat gaccttgagc ccgtccttct
      781 tcgccttctt ccagctgtcg cccttgcgga cgccgaccac gacgtcgtag ccggagtcct
      841 tgaggttcag cgcgtgcgcg cgaccctggc tgccgtagcc cacgatggcg atacgggcgc
      901 ccttcaaggc cttcctgtcc acgtccttct tcgagtacgc gcgcatgttc aactcctgag
      961 cgatgatcaa tggatgatgg tgggtgtcca aacgaagaac cccgcccggc tcgagtccgg
     1021 tgcggggttc ctggtatccg tctgcgcctt ggcgccttca gcccggggcc ccgcacctcc
     1081 cgaggggaag caggacgagc aggggaagca gcaggcccgg cagtcgggca gccgttgcga
     1141 ggtcgtgggc ggtcgtggcc tcaaactcca tcactggcct gctcacaagg gtcgatgcga
     1201 actgtggata gcagcatagg cccctatata ttgtcaaccg cactgcaaaa agaacttccg
     1261 ctaagctgtc accaccatgt tcgaagaaca cgacaccggg gccgcccggc cgctttggct
     1321 ggtcgatgag cagggactcg ccagctggct cgaatcgcag gatccgccga cgcggcagtg
     1381 gctgaccgcg atgcgattcc gcgccgagcg ccaccaggtg gcctgcatgc cggcggacgg
     1441 tggcggcgtg cgcatggcgg tcctcggtct cggcaggctg ccgggcctcg aatcactgga
     1501 gccatggcac ctggccggcg caccggaccg cctgccggcg ggagcctggc gcattgcaac
     1561 ggcgctgccc gcggccgcgg caacggcggc cgccctgggc tgggcctacg gcgcatacag
     1621 gttcgaacgc tatcgcgcga ggtccagtgc cgccgctggc gcggcggcct tggttcatcc
     1681 gcagctggcg gacatgaccc acgtgcggcg catggccggc gcactcgcga tggcccgcga
     1741 cctgatcaac acgcccgcgt cggacctcac gccggcgagc ctcgcggacc aggcgctgga
     1801 attggcggtt cgctgcggcg gccacgggca cgtcgtctcg ggagacgcgc tgcgggaggg
     1861 atttccggcg atccacgcgg tcggcagggc tgccgacacg gcgccacgcc tcgtcgactt
     1921 cagctggggc gatccggcgg cacctcgcgt gacgctggtc ggcaagggcg tctgcttcga
     1981 ctcgggcgga ctcgacatca agggttcggc cggaatgctg ctcatgaaga aggacatggg
     2041 tggcgccgcc tgcgtattgg cgctcgcccg cgtgatcatg gagagcgcgc tgcccgtgcg
     2101 actgcgagtc ctcattccgg cagtcgagaa ttccatcgcg ggcaatgcct accggccggg
     2161 cgacgtgatc cgcacgcgca aggggctggc cgtcgaggtc ggcaacacnn nnnnnnnnnn
     2221 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnntc ggcgacctca ataatgtgtc ggcgtcgcca
     2341 tttgccggcg cgatcatcgg cggcctgttc ctgcgccgct tcgtcacgca ggcgaaggac
     2401 tggctgcacg tcgatctcta cgcctggaac ggcaaggacc gtccgggccg gccggtcggc
     2461 gccgaaccgc aggccgtgcg tgcgctctac gcgctgctgg tcgaccgtta cggctgaccg
     2521 ccgaacgtga acctgcgcta cgagcgtcgc gcctggacgc tgatctacct gtgcctcggc
     2581 atgatcgagg gcggcaccgc cgccgtcgca gtgcgatcgt tgttcggcgc aaccgtgccg
     2641 gacctcgcgg tcgacctggt cgtcgcgctg gtgagttccg cgcccgcatg gtccaatctc
     2701 gcgagtcttg cgtacgcgcg cagggcgcag ggccgaccga aggtggcatt cctgcagccg
     2761 ctgctcttcg cggtcgccgg atgcgtcgcg gtgctgtcgc tcgttccccg gggtggcgcc
     2821 ggcctggtcg tgttcctggt ggcgtacgcc gcaagccggc tgctgtgggc gggtgtcgac
     2881 acggtgcgct cggtcatctg gagcgtgaac tatccgcggc acctgcgcgc gagcgtcgct
     2941 ggccggatca tggtcgcgag ctcgctggca ctggcgggca cgggcctgct gctgggctgg
     3001 ttgctgggtc gcggcggacc atggttccgg tttgccattg cgggcgccgc gtgttgcgga
     3061 cttgcgggcg ccgcagcctt cagtcgattc cgcgtgcgac gcgaggtcag cctgctcgcg
     3121 gccgaacggg cgcgcctcga tgaaggcgcg aggttcgacc ttgccgggat ccgcgacgtg
     3181 ctggcgcgcg acgccggctt tcgccactac atgtatgcga tgtcactgtt cggcgccggc
     3241 gtgctgtcgc tgccgccgct catgatcatc tgcctcggcg acgtactgca cctgtcggcg
     3301 ttcgtgcagg tggccgtgac gtcgtcgctg ccgatcctcg tcatgcccgt cgcgctgcag
     3361 ccctgggccc ggtttctcga tcgccaccac gtcgtcgtct accgctcggt gcacggtctg
     3421 gtcgggaccg ccgcgacgct gctgctcgtg tcagcggtcc tgtttcgtgc gccgctgttg
     3481 ctgtggcccg gagccgtgct gttcggcacg tcgctcgctg cgggaagcct cggctggaat
     3541 ctaggccaca acgacttcgc gccgcgaggc gaggagactc gctacatggc gctgcacgtg
     3601 acgctgacgg gcgtgcgcgg cctcctgatg ccgccgctcg cggtcgcgct ctatcacgcg
     3661 ctcgacgcgt ggcgcccagg cacgggtagc tgggcactgc tgctgccgct gctgctggtc
     3721 gccgcgggag cccgggagtt cgacgcgatg cggcggctag gtgatatccg caggccgccg
     3781 gcgtgaccag acgacgcttc cgacctcaga ataggcattg atctccagga gtcgcccgca
     3841 tgacccgacg caaggccgat ccgcgcgcct attcccgact cgtgtacgac ggcgtcaagc
     3901 agacgccgag ccgcgccatg ctgcgcgccg tcggctacac cgaccgcgac ttccgcaggc
     3961 cgcaggtcgg gatcgcatcg acgtggagca acctcacgcc gtgcaacatg cacatcgaca
     4021 agct
//
LOCUS       DAOMFD010000177         4020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10092_length_4020_cov_33.271375, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000177 DAOMFD010000000
VERSION     DAOMFD010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10092_length_4020_cov_33.271375"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="PL152_09070"
     CDS             complement(<1..407)
                     /locus_tag="PL152_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49477.1"
                     /translation="MTEKLGLSGRLARVFLDSELTPLLGLTALLLGLFAVFVTPREEE
                     PQINVTMANVIVPSPGASAQQVETLVATPMEKVLAEISGVKHVYSVSRPGVAVVTVQF
                     EVGQERTAAIVRLYNAIYSNADWRPPGLGVLQPL"
     gene            complement(400..1476)
                     /locus_tag="PL152_09075"
     CDS             complement(400..1476)
                     /locus_tag="PL152_09075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR49478.1"
                     /translation="MSQAVKAAQASVFLIALATLVPWMVAPAAGAPAGAPAGLATLRI
                     TAAAMPAERLLDGTVEAVNQTTVSAQTSGRIVAINFDVNDFVPAGTVIIRIRSTEQVA
                     SLTQAEAALKEATSREAEAQTHYERIHDMYERRVVAKATLDEASAARDAAVARLIAAK
                     AGLEAAREGVSYTEIRAPYSGVVTQKLVQVGQSVAPGTPLMAGASLDALRVIAEVPQS
                     VAEQVRKVRRAVVYVDGRRIESTGLTLYPAAQPETNTFRVRIELPKDVQGLAPGMFVK
                     VGLVTGEADLLLVPRSAVVERSEMRAVYVVAPDGRVTLRQVRLGHARGDQVEILAGLV
                     AGETIAVDPAAAALRARQQATADD"
     gene            complement(1496..1696)
                     /locus_tag="PL152_09080"
     CDS             complement(1496..1696)
                     /locus_tag="PL152_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HQR49479.1"
                     /translation="MSIDRIVLAFAGLMVLASLLLGLYVSHYWFWLTAFVGANLFQSA
                     FTGFCPLAAILRKAGVKPGAAF"
     gene            complement(1718..2083)
                     /locus_tag="PL152_09085"
     CDS             complement(1718..2083)
                     /locus_tag="PL152_09085"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HQR49480.1"
                     /translation="MTHHDSHITQATPEQVHAWLQTGEILLVDVREANEYAFERIHGA
                     LLYPLSTFDPKVLPRGSRIVLQCGSGKRSMMAAHRLEVTDHESIVNLAGGIQAWKAAG
                     LPVIRIDVQTGKVVDDGRR"
     gene            complement(2118..3008)
                     /locus_tag="PL152_09090"
     CDS             complement(2118..3008)
                     /locus_tag="PL152_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR49481.1"
                     /translation="MNRPSSVQPSVAPFHHPASGTWTYVVADPGTRAAAVIDPVLDYD
                     WRSGRTGTRAADAVIRHCREAGLEVRWILETHAHADHLSSAPYVQSRLGGVIAIGQGI
                     RKVQSTFKRVFGLGDDFTADGSQFDRLLADGEVVPVGDIAGKVIASPGHTNDSVAYLI
                     GDALFVGDTLFMPDSGSARCDFPGGDAVELYRTVQRFFALPGGTRVFVCHDYAPGGRE
                     PRCETTIAEQRSANIHVRDGVSEQDYVEMRTRRDATLDVPNLILPSIQVNIRGGRLPP
                     PGPDGVSYLRIPLNVVGRPI"
     gene            3087..3488
                     /locus_tag="PL152_09095"
     CDS             3087..3488
                     /locus_tag="PL152_09095"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR49482.1"
                     /translation="MTPVARSRTAARRRPPALDAMKDHAADAAQLLKTLGNEQRLLIL
                     CNLLERPMTVGELNQSVELSQSALSQHLALLREAGLVETRREAQSIHYSLPSGPVTKV
                     MALLQEIYCAPAGGRSAPSTPDRTRHGACRA"
     gene            3502..>4020
                     /locus_tag="PL152_09100"
     CDS             3502..>4020
                     /locus_tag="PL152_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HQR49483.1"
                     /translation="MLLPLLGALAIGLSLGLLGSGGSILTVPVLHYLVGQPDKAAIGG
                     SLLVVGLIAAAGCVPYALHRQVDWRNVAWFGLPGMGGAWVGATLARWVPGAAQLTLFA
                     AVMLVAAWRMLRGAIVTRTDHEPQLPAVIGGGIGVGLLSGLVGVGGGFLIVPALVLLA
                     GVPMASAVGTSLA"
BASE COUNT          560 a         1387 c         1462 g          611 t
ORIGIN      
        1 agcggctgca ggaccccgag gccgggaggg cgccagtcgg cgttcgagta gatggcgttg
       61 tacaggcgca cgatcgcggc ggtccgctcc tggccgacct cgaactggac cgtgacgacg
      121 gccaccccgg ggcgcgagac ggagtagacg tgcttcacgc cggagatctc cgcgagcacc
      181 ttctccatcg gcgtggcgac cagcgtctcg acctgctgcg cgctggcgcc gggcgaaggc
      241 acgatcacgt tggccatcgt gacgttgatc tgcggctctt cctcgcgggg cgtgacgaac
      301 accgcgaaca gcccgagcag cagcgcggtg agcccgagca gcggcgtgag ttcgctgtcg
      361 aggaacaccc gggcgaggcg cccgctcagg ccgagcttct cagtcatcgg cggtcgcctg
      421 ctgccgcgcg cgcagcgcgg cggcggccgg atcgaccgcg atcgtctcgc ccgccaccag
      481 gcctgcgagg atctcgacct ggtcgccgcg cgcatggccc aggcgcacct gcctgagcgt
      541 cacgcgcccg tcgggagcga cgacgtagac ggcgcgcatc tcgctgcgct cgacgacggc
      601 gctgcgcggc accagcagca ggtccgcctc gccggtcacg agcccgacct tgacgaacat
      661 gcccggggcg agtccctgga cgtctttcgg cagttcgatg cggacgcgga acgtgttcgt
      721 ctccggctgc gccgccgggt acagcgtgag gccggtcgat tcgatgcggc gaccgtcgac
      781 gtagacgacg gccctgcgca ccttgcgcac ctgctcggcg acgctctgcg ggacctccgc
      841 gatgaccctg agcgcatcga gcgacgcgcc cgccatgagc ggcgtcccgg gcgcgacgga
      901 ttggccgacc tgcaccagct tctgcgtcac gacgccggaa tacggcgcgc ggatctccgt
      961 gtaggagacc ccttcgcgag cggcctcgag gccggccttc gccgcgatca ggcgcgccac
     1021 ggccgcgtca cgcgcggcgc tcgcctcgtc cagcgtggct ttcgcgacca cgcggcgctc
     1081 gtacatgtcg tggatgcgct cgtagtgcgt ctgcgcctcc gcctcgcgcg acgtcgcctc
     1141 cttgagcgcc gcctcggcct gggtgaggct cgcgacctgc tccgtgctgc ggatgcggat
     1201 gatcacggta cccgccggga cgaagtcgtt cacgtcgaag ttgatggcga cgatgcgtcc
     1261 cgaggtctgc gccgaaaccg tcgtctggtt gaccgcctcg acggtgccgt cgagcaggcg
     1321 ctccgccggc atcgccgccg cggtgatcct gagcgtggcc agtccggccg gggcgccggc
     1381 cggtgcgccg gccgccgggg cgaccatcca ggggaccagc gtcgcgagcg cgatcaggaa
     1441 tacggacgcc tgagccgctt tgacagcttg tgacatggga ccgccgctgg ttggttcaga
     1501 atgcggcgcc cggcttcact ccggccttgc ggaggatcgc ggcgagcgga cagaagcccg
     1561 tgaaggccga ctggaagagg ttggccccga cgaacgcggt gagccagaac cagtagtggc
     1621 tcacgtacag gcccagcagc aggctcgcga gcaccatcag tcccgcgaag gccaatacga
     1681 tccggtcgat gctcatggtc gttctcctcc ggaggattca gcggcggccg tcgtcgacga
     1741 ccttgccggt ctggacgtcg atgcggatga ccggcagccc ggcggccttc caggcctgga
     1801 tgccgccggc gaggttcacg atggactcgt ggtcagtcac ctcgagcctg tgcgcggcca
     1861 tcatcgagcg cttgcccgag ccgcactgca gcacgattcg cgagccgcgg ggcaacactt
     1921 tcggatcaaa tgtcgacagc gggtacagca gcgcgccgtg gatgcgctcg aaggcgtatt
     1981 cgttggcctc gcgcacgtcg acgagcagga tctcgccggt ctgcagccag gcgtgcacct
     2041 gttcgggcgt cgcctgggtg atgtgggaat cgtggtgcgt catggttgac tcagcggcgg
     2101 gagggatgaa gcgggattca gatcggccgg cccacgacgt tgagcgggat ccggaggtag
     2161 ctcacgccgt ccggccccgg cggcggcagg cgcccgccgc ggatgttcac ctggatcgac
     2221 ggcaggatga ggttcggcac gtcgagcgtc gcgtcgcgcc gggtgcgcat ctcgacgtag
     2281 tcctgctcgc tgacgccgtc gcgcacgtgg atgttggccg agcgctgctc ggcgatggtc
     2341 gtctcgcagc gcggctcgcg gccgccgggc gcatagtcgt ggcagacgaa gacacgcgtc
     2401 ccgccgggca gcgcgaagaa gcgctgcacg gtccggtaca gctcgacggc gtcgccgccc
     2461 ggaaagtcgc agcgcgccga gccgctgtcc ggcatgaaca gcgtgtcgcc cacgaacagc
     2521 gcgtccccga tgaggtaggc gacgctgtcg ttggtgtggc ccggcgacgc gatgaccttg
     2581 cccgcgatgt cgccgaccgg gaccacctcc ccgtcggcga gcagccgatc gaactggctg
     2641 ccgtcggcgg tgaaatcgtc gcccagcccg aagacccgct tgaacgtcga ctgcaccttg
     2701 cggatgccct ggccgatggc gatgaccccg ccgagtcgcg actgcacgta cggcgccgag
     2761 gagaggtggt cggcgtgggc gtgcgtctcg aggatccagc gcacctcgag tcccgcctcg
     2821 cggcagtgac ggatgacggc gtcggcggcc ctggtgccgg tgcgtcccga ccgccagtcg
     2881 tagtcgagca ccgggtcgat caccgcggcc gcacgcgtgc cgggatcggc gaccacgtag
     2941 gtccacgtgc ccgacgccgg gtgatggaac ggtgcgaccg agggctgcac ggacgagggc
     3001 ctgttcatgg gcgggacgtc accatattag gctttgctaa tttagtcaac ggtgaactat
     3061 actgcgtggc agccgggatg agtcgcatga cgccagtcgc aagaagccgt accgccgcgc
     3121 gccgcaggcc gccggcgctc gacgccatga aggatcacgc ggcggacgcg gcgcagctgc
     3181 tcaagaccct cggcaacgag cagcggctgc tgatcctctg caacctcctc gagcgcccga
     3241 tgacggtggg cgagttgaac cagtcggtcg agttgagcca gtccgcgctg tcgcagcacc
     3301 tcgcgctgct gcgcgaggcg ggcctggtgg agacccggcg cgaggcccag tccatccatt
     3361 actcgctgcc ctccggaccg gtcacgaagg tgatggcgct gctgcaggaa atctattgtg
     3421 cgcctgccgg cgggcgatcg gcgccgtcca cgcccgaccg cacccgtcac ggcgcctgcc
     3481 gggcgtgatc cgggccggcg cttgctgctc ccactcctcg gcgcgctcgc catcggcctg
     3541 agcctgggac tgctgggctc gggcggctcg atcctgacgg tgccggtcct gcactacctc
     3601 gtcggccagc cggacaaggc ggccatcggc ggttcgctgc tggtcgtcgg cctgatcgcg
     3661 gccgcgggct gcgtcccgta cgcgctgcac cggcaggtgg actggcgcaa cgtcgcgtgg
     3721 ttcggcctgc cggggatggg cggcgcctgg gtaggcgcca cgctggcgcg ctgggtgccg
     3781 ggtgcggcgc agctcacgct gttcgcggcc gtgatgctgg tcgcggcgtg gcgcatgctg
     3841 cgcggcgcca tcgtcacgcg gacggaccac gagccgcaac tgccggccgt catcggcggt
     3901 ggcatcggcg tcgggctcct gagcggcctg gtcggcgtcg gcggcggttt cctgatcgtg
     3961 cccgcgctgg tgctgctcgc cggcgtgccg atggcgagcg cggtcgggac cagcctcgcc
//
LOCUS       DAOMFD010000178         4008 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10152_length_4008_cov_39.883380, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000178 DAOMFD010000000
VERSION     DAOMFD010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4008)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4008
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10152_length_4008_cov_39.883380"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="PL152_09105"
     CDS             <1..667
                     /locus_tag="PL152_09105"
                     /inference="COORDINATES: protein motif:HMM:NF012424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HQR49484.1"
                     /translation="ERSSMRDAIAAAMSKSKREIPHYYLSLAADVTVATQWLAARNAT
                     VPAGERLLFAALLVKAVALTCGEIEGFSGFYRDGSYAASPVAHVGVAVALRGGGLVAP
                     AIMDTAQKPLPALMEDFRQLVMRARAGRLRASELAAPTIILTSLGESTVDAVFPIIQP
                     PQVAIVGAGAVTQRAWVVEGRVEPRQVITLSLGADHRVTDGRRGAQFLARIAERLAAP
                     DAL"
     gene            678..923
                     /locus_tag="PL152_09110"
     CDS             678..923
                     /locus_tag="PL152_09110"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HQR49485.1"
                     /translation="MKREELVAKLLGMVTEIAPDVDAATIIPGVDFRDQFDFDSMDTL
                     NFAVAIHRELGVDVPEAEYSRLASLDKCVAYLTAKGI"
     gene            complement(968..1819)
                     /locus_tag="PL152_09115"
     CDS             complement(968..1819)
                     /locus_tag="PL152_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HQR49486.1"
                     /translation="MDIEGRRQSSNIEDQRGRRPVRTGIGLSIGGVLLLLALSYMGVD
                     VRPFLGMAVQQQASEPQSSQPGQPYQESPQEAKLRELTAVVLADTEDAWHAILPQYGV
                     QYVEPKLVLFTGAVQSACGGAEAAMGPFYCPSDQKVYIDLSFYDELSSRFGAPGDFAE
                     AYVVAHEVGHHVQNLLGKSEEVHAAQQRTPGKANELSVKLELQADCYAGVWAKNAAQS
                     RHLIEAGDIEEGLRAASAIGDDTLQRQAQGYVVPDAFTHGSSEQRVRWFKRGIENGTL
                     EACDTFQ"
     gene            complement(1830..2405)
                     /locus_tag="PL152_09120"
     CDS             complement(1830..2405)
                     /locus_tag="PL152_09120"
                     /inference="COORDINATES: protein motif:HMM:NF017819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF924 family protein"
                     /protein_id="HQR49487.1"
                     /translation="MNDSGQSPEPRLDSWVGQVLSFWFSDLDEADWWKKDPALDEKIR
                     SRFLGVHERLVESGAIEVTRPRPILAAVIVLDQFSRNMFRDTPRAYATDPIARRLARE
                     AITQGYDQGMRAEERMFLYLPFEHSEDRADQELSVELFSRLGRDDWTRFAIAHKVIID
                     RFGRFPHRNAVLGRVSSPEEIEVMRQPMGSF"
     gene            complement(2446..>4008)
                     /locus_tag="PL152_09125"
     CDS             complement(2446..>4008)
                     /locus_tag="PL152_09125"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HQR49488.1"
                     /translation="LEGQGCTVWWDPAITPGQEFDRLIAEQLACAAAVVVVWTQDSVV
                     SRWVRGEARDGADRGILVPVRFGKTALPIDFRAFHTIDLDEGADVSRSPAFREVLNAI
                     RVLVSKSRDASAEATAAAPMAIPASAGPARVAICVLPFANLGGDPEQEFFSDGITADI
                     ITELSRWRMLSVRPRSASFKYRGGAVDPMRVARDLNVNYVVEGSVRRIGGRIRISAQL
                     VDGETGSQVWGEKFDRAQAEIFDVQDQLVQTIVSTLVGRVQMSDADRARRKPPSSLAA
                     YECVQRGNALAWDDPMQAAEAVRLFGRAIELDPGYAMAYALLGTMRVGQWRNDAPGAD
                     AALDEAYELTKRAVELDDGDSTCHSLLAHACLYRRSYELALQHMRRSVELNPHNPWNR
                     ADLGLVLTYAGPAEEAVDWLRRAREIDPYFDPPWYWRQAGQALMVLHRFEEALAMFAH
                     VPLRTYRASAYIAACHARLGNAELARQSVAECLASRPEFTIRHFMSKEPFRNQDDAEY
                     LGESLRLAGLPE"
BASE COUNT          622 a         1390 c         1343 g          653 t
ORIGIN      
        1 cgagcgcagc agcatgcgcg acgcgatcgc cgcggcgatg tcgaagtcca agcgcgagat
       61 tccccactat tacctttcgc tcgccgcgga cgtgacggtc gcgacacagt ggctcgcagc
      121 gcgcaacgcc acggtgccgg ccggcgagcg gctgctgttc gcggcgctgc tcgtcaaggc
      181 cgtcgcgctc acttgtggcg agatcgaggg gttcagcggc ttctatcgcg acgggtccta
      241 cgcagcgagt ccggtcgcac acgtgggggt ggcggtcgcc ttgaggggcg gcgggctcgt
      301 cgcgccggcg atcatggaca cggcgcagaa gccactcccc gcgctgatgg aggacttccg
      361 gcagctggtg atgcgggcgc gcgccgggcg cctgcgggcg agcgagctcg cggcacccac
      421 gatcatcctc acgagtctcg gcgagtcgac ggtggacgcg gtgtttccga tcatccagcc
      481 gccgcaggtg gcgatcgtcg gcgcgggcgc ggtcacgcag cgtgcgtggg tcgtggaggg
      541 ccgggtcgag ccgcgacagg tgatcacgct gtcgctcggt gccgatcacc gggtgacgga
      601 cgggcgtcgt ggcgcgcagt tcctggcgcg gatcgccgag cggctggccg cacccgacgc
      661 gctgtgaggt gccgacgatg aaacgcgagg agctcgtcgc gaaactgctc ggaatggtga
      721 cggagatcgc gcccgacgtg gatgccgcga cgatcatccc gggcgtggat ttccgcgacc
      781 agttcgactt cgactcgatg gacaccctga atttcgccgt cgcgattcat cgcgaactcg
      841 gggtggacgt gcccgaggcc gagtactcgc gactcgccag cctcgacaag tgcgtcgcgt
      901 acctgaccgc caagggaatt taggggacat tcacctattt ctgatgacag aaattggtga
      961 atgtccccta ttggaacgtg tcgcaggcct cgagcgtgcc gttctcgatg ccgcgcttga
     1021 accagcggac gcgctgctcg gaggagccgt gcgtgaacgc gtcgggcacg acgtagccct
     1081 gcgcctgccg ctgcagcgtg tcgtcgccga tggcgctggc cgccctgagg ccttcctcga
     1141 tgtctcccgc ctcgatcagg tgccgtgact gggcggcatt cttagcccac acgccggcat
     1201 agcagtcggc ctgcagttcc agcttcaccg acagctcgtt ggccttgccg ggcgtgcgct
     1261 gctgggccgc gtgtacctcc tcggacttgc cgagcaggtt ctgcacgtgg tgcccgacct
     1321 cgtgtgcgac gacgtacgcc tcggcgaagt cgccgggcgc gccgaagcgt gacgacagct
     1381 cgtcgtagaa cgacaggtcg atgtacacct tctggtccga cgggcagtag aacggtccca
     1441 tcgccgcctc cgccccgccg cacgccgatt gcaccgcccc ggtgaacagc accagcttcg
     1501 gctcgacgta ctgcacgccg tattgcggca ggatcgcgtg ccacgcgtcc tcggtgtccg
     1561 ccagcacgac cgccgtcagt tcacgcagct tcgcttcctg cgggctctcc tggtacggct
     1621 ggccgggttg cgaagactgc ggctcggacg cttgctgctg caccgccatg ccgaggaacg
     1681 gccggacgtc cacgcccatg tacgacagcg cgagcagcag gagcacgccc ccgatgctga
     1741 gcccgatccc agtccgaacc ggacggcgac cccgctggtc ctcgatgttg ctgctctgcc
     1801 tgcgaccttc gatgtccatg gaaccatcct cagaacgagc ccatcggttg ccgcatcacc
     1861 tcgatctcct ccggcgacga tacgcgcccg agcacggcat tgcgatgcgg aaaccggccg
     1921 aagcgatcga tgatcacctt gtgcgcaatc gcaaagcgcg tccagtcgtc ccgtcccagc
     1981 ctcgagaaca attcgacgga aagttcctgg tccgcgcgat cctcgctgtg ctcgaacggc
     2041 aggtacagga acattcgctc ctcggcccgc atcccctggt cgtagccctg ggtgatcgcc
     2101 tcgcgtgcca gccggcgtgc gatcggatcg gtcgcgtacg cgcgcggagt gtcgcggaac
     2161 atgttgcgag agaactggtc gagcacgatg acggccgcga gtatcggtcg gggccgcgtg
     2221 acctcgatgg cgccgctctc caccagccgc tcgtgcacgc ccaggaagcg cgaccgaatc
     2281 ttctcgtcga gagcagggtc cttcttccac cagtccgcct cgtcgagatc ggagaaccag
     2341 aaggagagta cctgcccgac ccagctgtcg agacgcggct cgggcgattg accgctgtcg
     2401 ttcatgggat ccccttcgtc ggcaatgcgc acaacgtcag cctgatcact ccggcagccc
     2461 cgccagccgc agcgattccc cgaggtactc ggcgtcgtcc tggttgcgga acggctcctt
     2521 cgacatgaag tgccggatcg tgaattcggg ccgggatgcc aggcattccg cgactgattg
     2581 ccgcgcaagt tccgcgtttc cgagccgagc atggcaggcc gcgatgtacg ccgacgcccg
     2641 gtaggtgcgc aacgggacgt gcgcgaacat cgccagcgct tcctcgaagc ggtgcagcac
     2701 catcagcgcc tgtcccgcct gccgccagta ccacggtggg tcgaagtacg ggtcgatctc
     2761 ccgcgcgcgc cgcagccagt cgacggcttc ctccgcagga ccggcatagg tcagcacgag
     2821 gcccaggtcc gcgcggttcc aggggttgtg cggattgagc tccaccgagc ggcgcatgtg
     2881 ctgcagcgcg agctcgtagg accgccggta caggcacgcg tgcgccaaca gggagtggca
     2941 cgtactgtcc ccgtcgtcga gctcgacggc ccgcttggtc agctcgtacg cttcatcgag
     3001 cgccgcgtcc gcgcccggcg cgtcgttgcg ccactgcccg acacgcatcg tgccgagcag
     3061 cgcgtacgcc atggcgtagc ccggatcgag ctcgatggcg cgcccgaaca gtcggactgc
     3121 ctccgcggcc tgcatcggat cgtcccaggc gagcgcgttg ccgcgctgca cgcactcgta
     3181 cgcggcgagg ctggacggcg gcttgcggcg cgcgcgatcc gcatcggaca tctgcacccg
     3241 tccgaccagc gtgctcacga tggtctgcac cagctggtcc tgcacgtcga agatctccgc
     3301 ctgcgcgcga tcgaacttct cgccccacac ctggctgccg gtctcgccgt cgacgagctg
     3361 ggcgctgatg cggatgcggc cgccgatccg acgcacgctg ccttcgacca cgtagttcac
     3421 gttgagatcg cgcgcgacgc gcatcgggtc gacggcgccg ccgcggtact tgaacgacgc
     3481 cgagcgcggc cgcaccgaca gcatccgcca gcgcgacagc tcggtgatga tgtcggcggt
     3541 gatgccgtcg ctgaagaatt cctgctcggg gtcgccgccg aggttggcga acgggagcac
     3601 gcagatggcg acgcgggccg ggccagccga agcggggatc gccatcggtg ccgcggcagt
     3661 cgcctctgca ctcgcgtcgc gcgatttcga cacgagaacg cgaatggcgt tcagcacttc
     3721 ccggaaagcc gggctgcgcg atacgtcggc gccttcgtcc aggtcgatgg tgtggaacgc
     3781 gcggaagtcg atcggcaggg cggtctttcc gaaccggacc ggcaccagga tgccgcggtc
     3841 cgccccgtcg cgcgcttcgc cgcgcaccca gcgcgacacg acggagtcct gggtccacac
     3901 caccacgacc gcggcggcgc atgccagctg ttcggcaatc agccgatcga attcctgtcc
     3961 cggcgtgatg gccggatccc accacaccgt gcagccctgg ccctcgag
//
LOCUS       DAOMFD010000179         4002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10178_length_4002_cov_35.087155, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000179 DAOMFD010000000
VERSION     DAOMFD010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10178_length_4002_cov_35.087155"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1011)
                     /locus_tag="PL152_09130"
     CDS             complement(<1..1011)
                     /locus_tag="PL152_09130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49489.1"
                     /translation="MAALRLRESGPASGRLPIAMDRSRHAALFLAALACGVAAGCGGS
                     DAPASVALTPGPSPSPSPAPTPAPLPTPSPSPTPAPPLAPSPTPAPPTVDICPTTSGR
                     TLQVGPGKTYATPSAAAAAAQSGDRILIDPVDYRGDVATWSASNLTICGNGGRARLYA
                     DGKHAQGKGTWVIAVPNSATTTIVNVQFHDSKVPDKNGAGIRHEGGNLVLRNTGFFDN
                     ENGILGGGGPVTVTIERSEFARNGYGDGYSHNIYIGNVDRLRVIASWFHEAKIGHNLK
                     SRAKENIVENSYFMDGPTGTSSYLLDFPDGGVVFLRGNLLHKGPMADNTTAVAYGAER
                     NMW"
     gene            complement(1235..2374)
                     /gene="dnaJ"
                     /locus_tag="PL152_09135"
     CDS             complement(1235..2374)
                     /gene="dnaJ"
                     /locus_tag="PL152_09135"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HQR49490.1"
                     /translation="MSKRDYYEVLGVAKDASEQDITKAYRKLAMKYHPDRNTEGDAKS
                     SEEKFKEAKEAYEVLSDASKRAAYDRFGHAGLGQGAGGFGGGGPEGFGGFADAFGDIF
                     SDIFGGGARGGRNAVYRGADLRYSMEITLEQAAEGFATEIRVPSWDTCETCSGSGAKP
                     GTSAKTCGTCGGAGNVRMSQGFFSIQQTCPTCRGSGKVIPEPCTTCSGAGRVKKNKVL
                     EVAIPQGIDEGQRIRLAGKGEPGMNGGPHGDLYVEIHLKQHAVFQRDGDDLHCEVPIG
                     FTTAALGGEVEVPTLNGKASLEVGEGTQSGKTYRLRGKGIKGVRSSYPGDLYCHVSVE
                     TPVRLTEKQKKLLREFDSSLKAGGQKHSPQEKSFVDRMKGFFTAE"
     gene            complement(2480..3445)
                     /locus_tag="PL152_09140"
     CDS             complement(2480..3445)
                     /locus_tag="PL152_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HQR49491.1"
                     /translation="MSKRDFYEILGVPKGASDEDIKKAYRKLAMKYHPDRNKGRKDAE
                     TRFKEAKEAYEMLSDKDKRAAYDRFGHAGVDPSMAGAAGGAGGFDFNEVFRQSRGGQS
                     PGGGFEGFHFEGNPEDLFEGLFGAARGGRRGTAPRSRQGADIAYRMSVSLEQAARGFE
                     TMITVPGEGGSGERTLEVKIPAGIRDGQKVRVAGRGHPGHEGGPAGDLLVQIHVEPHA
                     RFEREGDDLVTRVTISQPTAALGGEIDVPTLDGHVTMTIPAGTQPGRRLRLRGKGMPT
                     MKSGSHGDLYVQVQVETPVHLTAEQKELYRRLDASLQGGGPARGT"
     gene            complement(3551..>4002)
                     /locus_tag="PL152_09145"
     CDS             complement(3551..>4002)
                     /locus_tag="PL152_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HQR49492.1"
                     /translation="KDKATGKENKITIKANSGLTEDEIQRMVKDAEANKAEDHKRFEL
                     AQARNMADAAVHEARKAATEAGDKAGAATKERVESAAKELEEAIKGDDKSVIEAKLKS
                     LTEAHQQLMAAVQAQGAQSAGAGAAPGGAKAEDEGVVDAEFKEVKRG"
BASE COUNT          543 a         1391 c         1306 g          762 t
ORIGIN      
        1 ccacatgttg cgctcggctc cgtaggccac cgcggtcgtg ttgtcggcca tcgggccctt
       61 gtgcagcagg ttgccgcgta ggaaaacgac accgccgtcg ggaaagtcga gcaggtaact
      121 ggaggtgccg gtcgggccat ccatgaagta actgttctcg acaatgttct ctttcgcacg
      181 gctcttcagg ttgtggccga tcttcgcctc gtggaaccag ctcgcgatga cgcgcaggcg
      241 atccacgttg ccgatgtaga tgttgtgcga atagccgtcg ccatagccgt tgcgcgcgaa
      301 ctcggagcgc tcgatggtga cggtgaccgg gccaccgccg ccgaggatgc cgttctcgtt
      361 gtcgaagaag ccggtgttgc gcaatacgag attgccgccc tcgtgccgga tgccggcgcc
      421 gttcttgtcc ggcaccttgg agtcgtgaaa ctgcacattg acgatcgtcg tcgtcgcgct
      481 gttgggcacg gcgatgaccc aggtgccctt gccctgcgca tgcttgccgt ccgcgtaaag
      541 ccgcgcgcgg ccgccgttgc cgcagatcgt caggttcgat gcactccagg tcgcgacatc
      601 gccgcggtag tccaccggat caatcaggat gcggtcaccg ctttgggcgg ccgctgccgc
      661 cgcgctcggc gtggcgtagg tcttgccagg tcccacctgc agcgtgcggc ccgaggtcgt
      721 cgggcagatg tcgacggtcg gcggcgcagg cgtcggtgat ggcgcgagcg gaggcgccgg
      781 ggtcgggctc ggcgatggcg tcggcaacgg cgctggcgtg ggtgcgggcg acggtgacgg
      841 gctcggcccg ggtgtcagcg ccacagaggc aggggcgtcc gatccgccgc agccggctgc
      901 aactccacat gccaatgccg cgagaaacag cgctgcgtgt cgtgatctgt ccattgcgat
      961 gggcaacctt cctgatgcag gcccgctctc acgcaggcga agtgccgcca ttttcgcagc
     1021 gcaggccggc gctaccggag cgttgcgtcc cgcactcaag cgatcaccga tggacgtcgc
     1081 gtgttcacgc gtcgcgcacg aggtatccga tgcctaatgc gccgtggcac gccgcccgcc
     1141 gcggccccgc acttgaatgc ccgtgaaggc gaccgtcgcg gcccgccact ccgaccaccg
     1201 acggcggagc gcggcacgcg agcgcccccg ggtgctactc ggcggtgaag aaccccttca
     1261 tgcggtcgac gaagctcttc tcctgcggcg aatgcttctg gccgccggcc ttcagcgacg
     1321 agtcgaactc gcgcagcagc ttcttctgct tttcggtcag gcgcaccggc gtctcgacgc
     1381 tgacatggca gtagagatca ccggggtagc tcgagcgcac gcccttgatg cccttgccgc
     1441 gcaggcggta ggtcttgccg gactgcgtac cttcgccgac ctcgaggctg gccttgccgt
     1501 tgagcgtcgg cacttcgacc tcgccgccga gcgcggccgt cgtgaagcca atcgggactt
     1561 cgcagtgcag gtcgtcgcca tcgcgctgga acacggcgtg ctgcttgagg tggatctcga
     1621 cgtagaggtc accgtgcggg ccgccgttca tgccgggctc gcccttgccg gccaggcgga
     1681 tgcgctggcc ctcgtcgatg ccctgcggga tcgcgacttc gagcaccttg ttcttcttga
     1741 cgcggcccgc gccgctgcag gtcgtgcacg gctccgggat caccttgccg ctgccgcggc
     1801 aggtcgggca ggtctgctgg atcgagaaga agccctgcga catgcgcacg ttgccggcgc
     1861 cgccgcaggt gccgcaggtc ttggcactgg tgcccggttt cgcgccggag cctgaacagg
     1921 tctcgcaggt gtcccagctg ggcacgcgga tctcggtcgc gaagccctcg gccgcctgct
     1981 cgagcgtgat ctccatgctg tagcgcaggt cagcaccgcg ataaacggcg ttgcgaccgc
     2041 cgcgcgcgcc gccgccgaag atgtcgctga agatgtcgcc gaaggcatcg gcgaagccgc
     2101 cgaagccctc cggcccgccg ccgccgaacc caccggcgcc ctgcccgagc cctgcatggc
     2161 cgaagcggtc gtaggccgcg cgcttgctgg cgtcggacag gacctcgtag gcttccttcg
     2221 cctccttgaa cttttcttcg gaagacttgg cgtcaccctc cgtgttgcgg tcggggtggt
     2281 acttcatcgc cagcttgcga taggccttgg tgatgtcctg ttcggacgcg tcctttgcta
     2341 caccgaggac ttcgtagtaa tcgcgcttag acatcgactc cagcaccctt tcgcttcaac
     2401 ccgtacgcgc cacgccacct ccacctgcgg tcttcgacgg ccgcattggc gaggaacgcg
     2461 tggcgcaaag caacgcagtt tacgtaccgc gcgccggtcc gccgccctgc aacgaggcgt
     2521 cgagccggcg atacagctcc ttctgttccg ccgtcaggtg cacgggcgtt tcgacctgca
     2581 cctgcacata gaggtcgccg tgcgacccgg acttcatcgt tggcatgccc ttgccgcgca
     2641 accgcaggcg gcgaccgggc tgcgtgccgg cggggatcgt catcgtcacg tggccatcga
     2701 gggtcggcac gtcgatctca ccacccagcg cggcggtcgg ctggctgatc gtgacgcgcg
     2761 tgacgaggtc gtcgccctcg cgctcgaagc gcgcgtgcgg ttcgacatgg atctgcacca
     2821 gcaggtcgcc ggccgggccg ccttcgtggc cgggatggcc gcgaccggcc acgcgcacct
     2881 tctggccatc gcggatgccg gcgggaatct tcacttcgag cgtgcgttcg ccgctgccgc
     2941 cttcgcccgg caccgtgatc atcgtctcga acccacgcgc agcctgctcg agcgaaacac
     3001 tcatgcggta ggcgatgtca gccccctggc gtgagcgcgg tgcggtgccg cgccggccac
     3061 cgcgggccgc accaaaaagg ccctcgaaca gatcttccgg gttgccctcg aagtgaaagc
     3121 cctcgaagcc gccgccgggg ctctggccgc cacgcgactg gcggaacact tcgttgaagt
     3181 cgaagccgcc cgctccgccc gcagcgcccg ccatcgacgg gtcgacaccg gcgtggccga
     3241 agcggtcata ggccgcgcgc ttgtccttgt cggacagcat ctcgtacgcc tccttggctt
     3301 ccttgaagcg cgtttcggcg tccttccggc ccttgttgcg gtccgggtgg tacttcatcg
     3361 cgagcttgcg atacgccttc ttgatgtcct cgtccgacgc acccttgggc acgccgagga
     3421 tctcgtagaa gtcgcgtttg ctcattgcgg tttcgtactc gtcatcaacg caaaagccgg
     3481 gtgcggcgtt acggcctgca cccggcggtt ccgggcacag gcccggcctt tgcggcgaac
     3541 gtccttgcgt ttagccgcgt ttcacttcct tgaactcggc atcgacgacg ccttcatctt
     3601 cggccttcgc tccgcccggt gccgcgcccg ctcccgccga ctgggcaccc tgcgcctgca
     3661 ccgcagccat cagttgctga tgcgcctcgg tcagcgactt gagcttggct tcgatcaccg
     3721 acttgtcgtc gcccttgatc gcctcttcca gttccttcgc ggccgattcg acgcgctcct
     3781 tggtcgcggc accagccttg tcgccggcct cggtcgccgc cttgcgcgcc tcgtgcaccg
     3841 cggcgtcagc catgttgcgg gcctgcgcca gttcgaagcg cttgtggtcc tcggccttgt
     3901 tggcttcggc gtccttgacc atgcgctgga tctcgtcttc ggtgagaccc gagttcgcct
     3961 tgatcgtgat cttgttttcc ttgccggtgg ccttgtcctt cg
//
LOCUS       DAOMFD010000180         3990 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10230_length_3990_cov_34.276747, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000180 DAOMFD010000000
VERSION     DAOMFD010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3990)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3990
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10230_length_3990_cov_34.276747"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="PL152_09150"
     CDS             complement(<1..342)
                     /locus_tag="PL152_09150"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR49493.1"
                     /translation="MNAPVVLVSGAAGGIGSAVVRRFAAAGWRVVATDRDAAGLERLQ
                     DGAVVARISGDVRVVADCRAVVEQAVAAAGRLDALVNAAGVWREGPVEDCSEADFDLV
                     VDVNLKGTYFLC"
     gene            complement(339..1079)
                     /locus_tag="PL152_09155"
     CDS             complement(339..1079)
                     /locus_tag="PL152_09155"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR49494.1"
                     /translation="MVTGAGSASGIGFAIARALGHAGYRLVMASTTDRIEQRATELRL
                     EGIDTRAVACDLTTDEGVAALAGAVGDSTVDVLVNNAGMAVLGEMDALTGLGDVSLAD
                     WHRSLERNLTTTFRVMRAFLPAMKRRGYGRIVNISSTTGAVAGVAGDSAYAAAKAGVV
                     GLTRSVALEAARYGVTVNAVAPGWIATGSQTAREAAAGARTPVGRSGTPEEVASVVAF
                     LASPGASYVTGQLIVVDGGNSVVEDLNA"
     gene            complement(1106..2161)
                     /locus_tag="PL152_09160"
     CDS             complement(1106..2161)
                     /locus_tag="PL152_09160"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: protein motif:HMM:NF009897.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HQR49495.1"
                     /translation="MHTLESDRQMQKPATTATSAQARPAFGTVLTDQMAVATFRDGRW
                     SAEEFRRTGPIEIHPAAHVLHYASTCFEGFKAYRWADGSVRVFRMDKHIERLRQSARL
                     LALPVPEAAQLGRMILSVIDRVRDQVPEPPGALYMRPLLYGTTPNIGAASIPANEAML
                     IVLASPVWDYFAGGMKPLRIFVEDAKHRTADHLGMVKTGGNYAAAMGPTLAARDQYKV
                     DQVLFCPGGSVQETGAANFLLLRKGHILTRSLDSTFLHGVTRDSLLTMAREMGFKVEE
                     RVFDVDEMLEWVKDGEAALSGTAAVLAGVGTLVHRGREHRVGNGDVGPVTKQLRAALV
                     AIQTGEAPDTHGWLTRV"
     gene            complement(2166..3458)
                     /locus_tag="PL152_09165"
     CDS             complement(2166..3458)
                     /locus_tag="PL152_09165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HQR49496.1"
                     /translation="MTRYVFKMPDLGEGTVEAEVVAWHVKVGDLVQEDQVMAEVMTEK
                     AAVEVPSPVTGRVVSLTGGPGDMVRVGAELIVFETESAEEHLPEAANGNGAQAPVAAP
                     PPKPVVVEPASAGRAEPVRPASVAVVAEPPRHGRVMASPATRRRAREAGIDLAQVNGS
                     GPSGRITRQDFEAALGGATAPKAPAGPTGTALMLERRAGTEEIKVIGVRRVIANRMTE
                     AKRNIPHFAYVEEVDVTELESLRQHLNERQPQGAPSLTFLPFIGAALVRVLRDFPQCN
                     ALYDAERNVLVRHHGVHLGVATQTPDGLKVPVVRHAEARTLWDLAAEIRRVSEAARSN
                     KAAREELAGSTITITSLGKLGGIVSTPIINAPEMAIIGVNKAVDRPVVVGGAIAVRRM
                     MNLSSSFDHRFVDGYDAAAMIQALKDLLEHPATIFIPG"
     gene            complement(3455..>3990)
                     /locus_tag="PL152_09170"
     CDS             complement(3455..>3990)
                     /locus_tag="PL152_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HQR49497.1"
                     /translation="IEDDDPVVFFEPKRIYNGPFDGEPNKPAVPWSTHPKGEVPDGYY
                     TVPIGKAEVVRKGTQVTIVTYGTMVHVCETAARGLGLDAEIIDVRTMVPLDIDTLCTS
                     VRKTGRCLIAHEATRFGGYGGELSAVIQEECFWSLEAPILRVAGWDTPYPHAFEWEYF
                     PGPQRVAAGLKAVMETP"
BASE COUNT          578 a         1423 c         1368 g          621 t
ORIGIN      
        1 gcacagaaaa taggtgccct tgaggttgac gtcgacgacc aggtcgaagt cggcctcgct
       61 gcagtcttcg acgggccctt cgcgccacac gccggccgcg ttgaccaggg cgtcgaggcg
      121 acccgcggcg gctaccgcct gctcgacgac ggcacgacag tcggcgacca cgcgcacgtc
      181 cccggaaatt cgcgcgacca ccgcgccgtc ctgcaggcgc tcgaggcccg ccgcgtcccg
      241 gtccgttgcg acgacccgcc agccagcggc tgcgaatctc cgtacgaccg ccgagccgat
      301 gccgccggcg gcaccgctca cgagcacgac gggagcgttc acgcgttcag gtcctcgacc
      361 accgagttgc cgccgtcgac cacgatcagc tgcccggtca cgtaggacgc gccgggcgac
      421 gccaggaaag cgacgacgct cgcgacctcc tccggcgttc ccgaccgacc cacgggtgtc
      481 cgcgccccgg ccgcggcctc gcgcgcggtc tgcgatccgg tcgcgatcca gcccggggcg
      541 accgcattga cggtgacacc gtagcgcgcc gcttcgagcg caaccgatcg cgtgagaccc
      601 acgacgccgg ccttcgcggc ggcataggcc gaatcgcctg caacaccggc gacggcaccc
      661 gtcgtggacg agatgttgac gatgcggccg taaccgcggc gcttcatggc gggcaggaag
      721 gcgcgcatga cgcggaaggt cgtggtgaga ttgcgctcca gcgagcgatg ccagtcggcg
      781 agcgacacgt cgccgaggcc ggtcagtgca tccatctcgc ccagcacggc catccccgcg
      841 ttattgacca gcacgtcgac cgtcgaatcg cccacggcac ccgccagggc ggcaacaccc
      901 tcgtcggtcg tgagatcgca ggcgacggcg cgggtatcga tcccttccag ccgcagctcc
      961 gtcgcgcgct gctcgatccg gtccgtcgtg gatgccatga cgagccggta gcccgcgtgc
     1021 cccagcgccc gcgcgatggc gaagccgatg ccggaggcgc tgcctgcccc ggtgacgatc
     1081 gcgagcgatg gctgacgttg cgtgctcaga cgcgggtcag ccagccgtgc gtgtccggcg
     1141 cctcgccggt ctggatggcg acgagcgcag cacgcagctg cttggtcacc gggccgacgt
     1201 cgccattgcc gacgcgatgc tcacgaccgc ggtgcaccag cgtgccgacg ccggcgagca
     1261 ccgcagcggt gccggagagc gccgcctcgc cgtccttgac ccactccagc atctcgtcga
     1321 cgtcgaagac gcgctcctcg accttgaatc ccatctcgcg ggccatggtg agcagcgagt
     1381 cgcgcgtgac gccgtgcagg aaggtcgagt ccaggctgcg cgtgaggatg tgtcccttgc
     1441 gcagcagcag gaagttcgcg gcgcccgttt cctgcaccga tccgcccggg cagaacagca
     1501 cctggtccac cttgtactgg tcgcgcgcgg cgagcgtcgg gcccattgcc gctgcatagt
     1561 tgccgccggt cttcaccatg ccgaggtgat ccgccgtgcg gtgcttcgcg tcctcgacga
     1621 agatcctgag cggcttcatg ccgccggcga agtagtccca cacgggactc gcgagcacga
     1681 tcagcatcgc ctcgttcgcg ggaatcgacg cggcgccgat gttgggcgtc gttccgtaca
     1741 ggagcggccg catgtagagc gcgcccggtg gctccggcac ctggtcacgc acgcggtcga
     1801 tcaccgagag gatcatgcgc ccaagctgcg ccgcctccgg caccggcagc gcgagcaggc
     1861 gcgcgctctg ccgcaggcgc tcgatgtgct tgtccatgcg gaacacgcgc acgctgccgt
     1921 ccgcccagcg ataggccttg aagccctcga agcacgtgct cgcgtaatgc aggacatggg
     1981 ccgccgggtg gatctcgatc gggccggtgc gtcggaattc ctcggcgctc caccggccgt
     2041 cgcggaacgt ggcgaccgcc atctggtcgg taagcaccgt gccgaaggcc ggccgggcct
     2101 gggcgctggt cgccgtggtt gcgggcttct gcatctgtcg gtcggattcg agggtgtgca
     2161 tctggctaac cgggaatgaa gatggttgcg gggtgttcga gcaggtcctt gagcgcctgg
     2221 atcatcgcgg cggcatcgta tccgtccacg aagcggtgat cgaatgaaga cgacagattc
     2281 atcatccgcc gcacggcaat cgcaccgccg acgacgaccg ggcggtcgac ggccttgttg
     2341 acgccgatga tcgccatctc cggcgcgttg atgatcggcg tggagacgat gccaccgagc
     2401 ttgccgaggc tcgtgatggt gatggtcgaa ccggcgagtt cctcgcgcgc tgccttgttg
     2461 ctgcgcgccg cctcggacac gcggcggatt tccgcggcaa ggtcccacag tgtgcgcgcc
     2521 tcggcgtggc gcacgaccgg gaccttgagg ccgtcgggcg tctgcgtggc gacgccgagg
     2581 tgcacgccgt ggtggcgcac cagcacgttg cgttcggcgt cgtacagggc attgcactgc
     2641 gggaagtcgc gcagcacgcg caccagcgcc gcgccgatga acggcaggaa cgtgagggac
     2701 ggcgcaccct gcggctggcg ctcgttcagg tgctggcgca gcgactcgag ttccgtgacg
     2761 tcgacttcct cgacgtacgc gaagtgcgga atgttgcgct tggcctccgt catgcggttc
     2821 gcgatgacgc gccgcacgcc gatgaccttg atctcctccg tgccggcgcg ccgctccagc
     2881 atcaacgccg tgcccgtcgg cccggcgggt gccttcggcg cggtcgcgcc accgagcgcg
     2941 gcctcgaagt cctggcgcgt gatgcggccg ctcgggcccg agccgttcac ctgcgcgagg
     3001 tcgatgcccg cttctcgcgc gcggcggcgc gtcgcaggcg acgccatcac tcgaccatgc
     3061 ctcggcggct cggccaccac ggccacgctc gcaggccgca cgggttcggc tcgaccggcg
     3121 ctcgccggtt ccacgaccac gggcttcggc ggcggcgcgg cgacgggcgc ctgcgcaccg
     3181 ttgccgttcg ccgcttcagg cagatgctct tccgctgact ccgtctcgaa gacgatgagt
     3241 tcggcgccga cgcgcaccat gtcgccgggc ccgccggtca gcgacaccac gcgaccggtc
     3301 accggcgacg gcacctcgac cgcggccttc tcggtcatga cctcggccat cacctggtct
     3361 tcctgcacga ggtcgccgac cttgacgtgc caggccacca cttcggcttc gaccgtgccc
     3421 tcgccgaggt ccggcatctt gaacacgtag cgggtcatgg cgtctccatc acggccttga
     3481 gtccagcggc gactcgttgc ggtccgggga agtattccca ctcgaaggcg tgaggatacg
     3541 gcgtgtccca gccggcgaca cgcaggatcg gggcctcgag gctccagaag cactcttcct
     3601 ggatgacggc cgacagctcg ccgccatagc cgccgaagcg cgtcgcctcg tgcgcgatga
     3661 ggcagcggcc ggtcttgcgg acggaggtgc agagcgtgtc gatgtcgagc ggcaccatcg
     3721 tgcgcacgtc gatgatctcg gcgtcgagcc cgagcccgcg cgccgcggtc tcgcagacgt
     3781 ggaccatcgt gccataggtg acgatcgtga cctgcgtgcc cttgcgcacg acctcggcct
     3841 tgccgatcgg cacggtgtag tagccgtcgg gcacctcgcc cttcgggtgc gtgctccacg
     3901 gcaccgcggg cttgttcggc tcgccgtcga acgggccgtt gtagatgcgc ttcggctcga
     3961 agaacaccac cgggtcgtcg tcctcgatcg
//
LOCUS       DAOMFD010000181         3984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10259_length_3984_cov_29.115806, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000181 DAOMFD010000000
VERSION     DAOMFD010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3984
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10259_length_3984_cov_29.115806"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..526)
                     /locus_tag="PL152_09175"
     CDS             complement(<1..526)
                     /locus_tag="PL152_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR49498.1"
                     /translation="MRRKDTQPRIAIIGAGPAGLSAAWYLKRNGYRSVRVFEKTDRVG
                     GKCLTFDYEGHQFDLAAHEMLAGYGDVMEIAKALGVPTVGYQNVLVYDTLGRRYMSML
                     EASQSGGYGRLQVVWASLRYTWMLLTRYREFARPGSGYADAPPELLQPLSSWIQEKRL
                     GALLETVLYVMKVQG"
     gene            complement(523..1890)
                     /locus_tag="PL152_09180"
     CDS             complement(523..1890)
                     /locus_tag="PL152_09180"
                     /inference="COORDINATES: protein motif:HMM:NF013739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR49499.1"
                     /translation="MRSDGIARDARILIVGAGAAGICTAWYLKLAGFRHVKVFEKSPR
                     LGGKCRSLTVDGQSFDLGANYVTSAYARVRELAAHVGATMYTERAGHVIDVADDWKIR
                     SILSEVLRRTSFPTLAWQSLRYLWKRWRIRKLLAPYKPGFGHVKDCPELQGSFEQWLR
                     RHRLDALIEIFEIPLTLMGYGKLDGIAAAYALTYMSPLTFKDLSMFAANLPLRCWPKR
                     FTQGYGRMFERLAAEVDVLTGATITKITRDDAIRVEYTLLEQDMEGDDTPGERATFDY
                     LVLACPQVPGVLREFLTLTPAEERLFGQVVLNPFFVTTYEAPGTERVAAVTFSLDEPT
                     VGQPFVVTRQYPESDFISVYTRGDHRQKISHEEVLAHNRRFLAGIGARDPDVEASTMD
                     DWAYFPHVPVEAMDAGFFAELEALQGTNRTFYCGGLLAFELVETIAEYSHDLVRRHFV
                     GKGTA"
     assembly_gap    2080..2179
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2180..>3984
                     /locus_tag="PL152_09185"
     CDS             <2180..>3984
                     /locus_tag="PL152_09185"
                     /inference="COORDINATES: protein motif:HMM:NF025056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HQR49500.1"
                     /translation="SADRQLIAPLVEFLAAQGWDVWWDRRIEAGEVFDRRIGQALAAA
                     DCVVVVWSRASIESNWVLSEAMAGFESKRLVPVALDARLQVPLPFNRLHAASLVDWSG
                     AQDHPGLAELAAGIRATVETQGMRGTHTVDAPAVRKPVVAVLPFDDLDTASSPRPACS
                     VIATRLIAALGHFSGLETISRRASFDAGLQSQEIRAVARSLRADFVVTGNVSGTGTAM
                     VIGAELIEGSSGRQCWSTSLVPDAGGALDPEAAAGAIARELSGEFLRLGRDRARDGAA
                     RGDALAAVEASRDTLLKSSRAAIAATRADARRALALSPDSGQAHALLASAIAEEIVNG
                     YADDLDAACAEARRAAEQAITLCPDDHAVLKYAGHVLATCGDHEQGERVLRRALELNR
                     YDDGTRGYLGWVLAPSTSTGHLDEIQATLDKLLAGTGKHPGRPFWLLHRSVALTCAGD
                     FEGALTAARTAVNFSPNLALAWLHAVNALGQLGRESEARALVERCPLDLARAGVSWEA
                     VIRLISRDEAAAERRIAGLRTAGLVGAHRGDSRVDTEERLSEDFTSLWRRVSCRDELA
                     AGSVRRAVFDRYREPARRYHTLAHIRHCLEQADLAA"
BASE COUNT          588 a         1368 c         1319 g          609 t
ORIGIN      
        1 agccctggac cttcatcacg tacagcacgg tctcgagcag cgcgccgagc cgcttttcct
       61 ggatccagct cgacagcggc tgcaggagct cgggcggcgc atcggcatac cccgacccgg
      121 gtcgcgcgaa ctcccggtag cgcgtgagca gcatccacgt gtagcgcagc gaggcccaca
      181 cgacctgcag gcgaccgtac ccgccggact gcgatgcctc gagcatgctc atgtagcgac
      241 ggccgagggt gtcgtagacc agcacgttct ggtagcccac ggtcgggaca ccgagggcct
      301 tggcgatctc catgacgtcg ccgtatccgg cgagcatctc gtgcgcggcg aggtcgaact
      361 gatggccttc gtagtcgaag gtcaggcact taccgccgac ccggtcggtc ttctcgaaca
      421 cccgcacgct gcgatagccg ttgcgcttca ggtaccaggc cgcgctcagc ccggcgggac
      481 ccgccccgat gatggcgatg cgtggctgcg tgtctttcct tctcatgccg tccccttgcc
      541 gacgaaatgc ctgcgcacga ggtcgtgcga atactcggcg atcgtctcca ccagctcgaa
      601 cgcgagcagc ccgccgcagt agaacgtgcg gttggtgccc tgcagggcct cgagctcggc
      661 gaagaagccc gcatccatcg cctcgaccgg cacgtgcggg aaatacgccc agtcgtccat
      721 cgtggaggcc tctacatccg ggtcgcgtgc gccgatcccg gcgaggaacc tgcggttgtg
      781 cgccaggact tcctcgtggc tgatcttctg ccggtgatcg ccgcgagtgt acacggagat
      841 gaagtcgctc tccgggtact ggcgcgtgac gacgaatggc tggcccacgg tcggctcgtc
      901 cagcgagaag gtcacggcgg cgacccgctc ggtgccgggc gcttcgtagg tggtcacgaa
      961 gaacgggttc agcacgacct ggccgaacag ccgttcctcc gctggcgtca atgtgaggaa
     1021 ttcgcgcagc acgccgggca cctgcgggca cgcgaggacg aggtaatcga acgtcgcacg
     1081 ctcgcccggt gtgtcgtcgc cctccatgtc ctgctcgagc agcgtgtact cgacgcggat
     1141 cgcgtcgtcg cgtgtgatct tcgtgatcgt cgcgccggtg agcacgtcca cctcggccgc
     1201 caggcgctcg aacatgcgcc cgtagccctg cgtgaagcgc ttcggccagc agcgcagcgg
     1261 caggttcgcg gcgaacatcg agaggtcctt gaacgtgagc gggctcatgt aggtgagcgc
     1321 gtaggcggcg gcgatgccgt cgagcttgcc gtagcccatc agcgtgagcg gtatctcgaa
     1381 gatctcgatc agggcatcga gccggtgcct gcgcagccat tgctcgaagc tgccctgcag
     1441 ctccgggcag tccttgacgt ggccgaagcc cggcttgtaa ggcgcgagca gcttgcggat
     1501 gcgccagcgc ttccacaggt accgcagcga ctgccacgcc agcgtcggaa acgacgtgcg
     1561 gcgcaggacc tcgctcagga tggagcggat cttccagtcg tcggcgacgt cgatcacgtg
     1621 gccggccctt tccgtgtaca tcgttgcgcc cacgtgcgcc gcgagctccc gcacgcgcgc
     1681 gtacgccgag gtgacgtagt tcgcgccgag atcgaaggac tggccgtcca ccgtaagcga
     1741 gcggcacttg ccgccgagcc gcggcgactt ctcgaatacc ttgacgtggc ggaagcccgc
     1801 gagcttgaga taccacgcgg tgcagatccc cgccgcaccc gcacccacga tcaggattcg
     1861 cgcatcgcgc gcgataccgt ccgaacgcat tcccgccccc ctgcccgaca ttctgcgtgc
     1921 cgttgccgcg ccggattcaa tacactgcgc gcctgcacaa cgcaacgggg atcggtttgg
     1981 ccagcagccc gcccaaggtc acgcggctct tcctcagcta cgcctcggcg gaccgtcagc
     2041 tgatcgcgcc gctggtggaa ttcctcgccg cacagggctn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2161 nnnnnnnnnn nnnnnnnnnt cggcggaccg tcagctgatc gcgccgctgg tggaattcct
     2221 cgccgcacag ggctgggacg tgtggtggga ccggcgcatc gaggcgggcg aggtcttcga
     2281 ccggcgcatc gggcaggcgc tggccgccgc cgactgtgtc gtcgtcgtgt ggtcgcgtgc
     2341 atcgatcgaa tcgaactggg tcctgagcga ggccatggcc ggtttcgagt cgaagcggct
     2401 ggttcccgtc gcgctcgacg cacgactgca ggttcccctg cccttcaacc gcctccacgc
     2461 cgcgtcgctc gtcgactgga gcggcgccca ggaccacccg ggcctggcgg aactcgccgc
     2521 cgggatccgc gccaccgtcg agacgcaggg catgcgcggc acccacaccg tggatgcgcc
     2581 cgcggttcgc aagccggtcg tcgccgtcct gccattcgac gacctcgaca cggcctcctc
     2641 gccacgcccg gcgtgctcgg tcatcgcaac gcgcctcatc gccgcgctcg ggcacttctc
     2701 gggcctcgag acgatttccc gccgcgcgag cttcgatgcc ggcctgcagt cgcaggagat
     2761 ccgcgcggtg gcccggtcgc tgcgcgcgga cttcgtcgtc accggcaacg tgtcgggcac
     2821 cggcaccgcc atggtgatcg gcgcggaact catcgaaggc agcagcgggc ggcagtgctg
     2881 gagcacgagc ctcgtgccgg acgcgggcgg cgcgctcgat ccggaggccg cagcgggggc
     2941 gatcgcgcgg gaattgagcg gcgagttcct gcggctgggc cgcgatcggg ctcgcgatgg
     3001 agcagcacgc ggcgacgcgc tcgccgccgt cgaggcgagc cgcgacacgc tgctcaagag
     3061 cagccgggca gcgatcgcag cgaccagggc ggatgcacgg cgcgcgctcg cactctcccc
     3121 ggacagcggg caggcgcacg cgctgctggc ctccgcaatc gctgaggaga tcgtcaatgg
     3181 ctacgcggac gatctcgatg ccgcgtgcgc cgaggcacgc cgcgcggccg aacaggcgat
     3241 cacgctgtgt ccggacgatc acgcggtcct caagtacgcc gggcacgtgc ttgcgacctg
     3301 tggcgatcac gagcagggtg aacgcgtcct gcgtcgcgcg ctggaactca atcgctacga
     3361 cgacggcaca cgcggctacc tcggctgggt gctcgcgccg tcgacgtcga ccgggcacct
     3421 cgacgagatc caggccacgc tcgacaaact gctggccgga accggcaagc atccgggacg
     3481 gccgttctgg ctgctgcacc gttccgtggc gctcacctgc gccggcgact tcgaaggagc
     3541 cctgaccgcg gcacgcaccg ccgtcaattt cagtcccaac ctggcgctcg cctggctgca
     3601 cgccgtgaac gcgctcggtc agctcgggcg tgagtccgag gctcgcgccc tggtcgagcg
     3661 ctgtccgctc gacctcgcgc gcgccggcgt gtcgtgggag gccgtgatcc ggctgatttc
     3721 gcgggacgag gcagcggccg aacgccgcat tgcggggctg cgaaccgcgg gactcgtcgg
     3781 agcgcaccgt ggcgactcgc gcgtcgacac ggaagagcgc ctgtcggagg acttcacgtc
     3841 attgtggcgc agggtgtcct gccgtgacga actcgccgcc gggagcgttc gccgcgccgt
     3901 cttcgaccgc tatcgcgagc cggcgcgtcg ctatcacacc ctcgcccaca tccggcactg
     3961 cctcgagcag gccgatcttg ccgc
//
LOCUS       DAOMFD010000182         3983 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10264_length_3983_cov_31.313900, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000182 DAOMFD010000000
VERSION     DAOMFD010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3983)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3983
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10264_length_3983_cov_31.313900"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="PL152_09190"
     CDS             complement(<1..625)
                     /locus_tag="PL152_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrpF domain-containing protein"
                     /protein_id="HQR49501.1"
                     /translation="MSPISLPQIRIPATYMRGGTSKGVFFRLQDLPEAAQVPGEARDR
                     LFMRVIGSPDPYGAHIDGMGGATSSTSKVVILSRSATADHDVDYLYGQVSIDKAFVDW
                     SGNCGNLSTAAGAFAIHAGLVDPSRIPANGTCVVRIWQANIGKTIIAHVPVTNGQVQE
                     TGDFELDGVTFPAAEIVLEFIDPAADDDGGGGAMFPTGNLVDDLEVPG"
     gene            complement(630..3248)
                     /gene="acnD"
                     /locus_tag="PL152_09195"
     CDS             complement(630..3248)
                     /gene="acnD"
                     /locus_tag="PL152_09195"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047456 - 2-methylisocitrate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0019629 - propionate catabolic process,
                     2-methylcitrate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe/S-dependent 2-methylisocitrate dehydratase
                     AcnD"
                     /protein_id="HQR49502.1"
                     /translation="MNSAHRKPLPGTALDYYDAREAVEAIRPGAWETLPYTARVHAEN
                     VVRRADPAIVTECLRQIVERKRERDFPWFPARVVCHDILGQTALVDLAGLRDAIAARG
                     GDPAKVNPVVPVQLIVDHSLAVECGGYDPDAFAKNRAIEDRRNEDRFHFIDWTKLAFD
                     NVDVIPAGNGIMHQINLEKMSPVIHAQDGVAFPDTCVGTDSHTPHVDALGVIAVGVGG
                     LEAENVMLGRASWMRLPEIVGVELKGRRQPGITATDIVLALTEFLRKSKVVGAYLEFR
                     GEGAASLTLGDRATISNMAPEYGATAAMFAIDSQTLQYLRLTGRSDEQVKLVETYAKT
                     AGLWADSLQQAEYERTLEFDLSSVVRNMAGPSNPHARVATADLAAKGIAGSWEEVPGQ
                     MPDGAVIIAAITSCTNTSNPRNVIAAGLLARNANRLGLKRKPWVKSSLAPGSKTVALY
                     LEEAGLKSELEQLGFGIVAFACTTCNGMSGALDPKIQQEIVDRDLYATAVLSGNRNFD
                     GRIHPYAKQAFLASPPLVVAYAIAGTIRFDIERDALGKDKDGKPVTLKDIWPSDEEID
                     AIVASSVKPEQFRKVYVPMFERKARTTAPVSPLYDWRPQSTYIRRPPYWEGALAGERT
                     LAGMRPLAVLGDNITTDHLSPSNAILRDSAAGEYLEKMGLPEEDFNSYATHRGDHLTA
                     QRATFANPTLVNEMAVVDGKVKKGSLARVEPDGRVMRMWEAIETYMDRKQPLIIIAGA
                     DYGQGSSRDWAAKGVRLAGVEAIVAEGFERIHRTNLIGMGVLPLEFKQGVNRKTLGLD
                     GTETYDVIGARKPRADLGLVIHRRSGETLQVPVTCRLDTAEEVSIYEAGGVLQRFAND
                     FLEGNRGHSPISAIRK"
     gene            complement(3250..>3983)
                     /locus_tag="PL152_09200"
     CDS             complement(3250..>3983)
                     /locus_tag="PL152_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HQR49503.1"
                     /translation="LCYWYHYSHSGQRINVETEDDSVGGHFLHLLHRRQPPESWVKAM
                     HVSLNLYAEHEFNASTFAARVIAGTGSDMYSAVTGAIGALRGPKHGGANEVAFEVQKR
                     YENPDEAEADIRRRVEAKEVIIGFGHPVYTIGDPRNKVIKEVARGLSKEAGDMKMFDI
                     AERLESVMWDIKKMFPNLDWFSAVSYHMMGVPTAMFTPLFVISRTTGWAAHVIEQRID
                     GKIIRPSANYTGPEDRKFVPIAKRK"
BASE COUNT          597 a         1343 c         1324 g          719 t
ORIGIN      
        1 cgcccggcac ctcgaggtcg tccacgaggt tgccggtcgg gaacatggcc ccgccaccac
       61 cgtcgtcgtc tgcggctgga tcgatgaact cgagcacgat ctccgcggcc gggaaggtca
      121 ccccgtcgag ctcgaagtca ccggtttcct gcacctggcc gttcgtgacc ggcacgtgcg
      181 caatgatggt cttgccgatg ttcgcctgcc agatgcgcac cacgcaggtc ccgttcgcag
      241 ggatgcgcga gggatcgacg aggcccgcgt ggatggcgaa tgcgccggcg gccgtcgaga
      301 gattgccgca gttgccggac cagtcgacga aggccttgtc gatcgacacc tggccgtaga
      361 ggtagtccac gtcatggtca gcggtggcgc tcctgctgag gatcaccacc ttgctggtgc
      421 tggacgtggc gccccccatg ccgtcgatgt gcgcaccgta ggggtccggg ctgccgatga
      481 cgcgcatgaa caaccggtcg cgcgcctcgc ccggcacctg cgccgcctcg ggcaggtcct
      541 gcaggcggaa gaacacgccc ttgctcgtgc cgccacgcat gtaggtggcg ggtatgcgga
      601 tctgcggcag ggaaataggg gacattctcc tatttccgaa ttgcggaaat aggagaatgt
      661 cccctatttc cctcaaggaa atcgttcgcg aagcgctgca gcacgccgcc cgcctcgtag
      721 atcgaaactt cctcggccgt gtcgagtcga cacgtcaccg gcacctgcag cgtctcgccg
      781 ctccgacggt ggatgacgag gcccaggtcc gcgcgcggct tgcgcgcgcc gatcacgtcg
      841 taggtctcgg tgccgtccag ccccagggtc ttgcgattca cgccttgctt gaactcgagc
      901 ggcagcacgc ccatcccgat caggttggtg cggtggatgc gctcgaagcc ctcggccacg
      961 atcgcttcca cgcccgcgag ccgcacgccc ttcgccgccc agtcgcgcga cgagccctgc
     1021 ccatagtccg cgccggcgat gatgatgagc ggctgcttgc ggtccatgta cgtctcgatt
     1081 gcttcccaca tgcgcatgac ccgaccgtcc ggctcgacgc gggcgagcga gcccttcttc
     1141 accttgccgt cgaccaccgc catctcgttc acgagcgtcg gattggcgaa cgtcgcgcgc
     1201 tgcgccgtga ggtggtcgcc gcggtgcgtc gcataggaat tgaagtcctc ttccggcagg
     1261 cccatcttct ccaggtactc gcccgccgcg ctgtcgcgca ggatcgcgtt cgacggggac
     1321 aggtgatcgg tcgtgatgtt gtcgccgagc accgcgagcg ggcgcatccc ggcgagcgtg
     1381 cgttcgccgg ccagcgcgcc ttcccagtac ggcggacggc ggatgtaggt gctctgcgga
     1441 cgccagtcgt acagcgggct gaccggagcc gtggtcctcg ccttgcgctc gaacatgggc
     1501 acgtagacct tgcggaactg ctccggcttg acgctcgagg cgacgatcgc gtcgatctcc
     1561 tcgtcggacg gccagatgtc cttcagcgtc accggcttgc cgtccttgtc cttgccgagt
     1621 gcatcgcgct cgatgtcgaa gcggatcgtc ccggcgatcg cgtacgcgac gaccagcggt
     1681 ggcgaggcga ggaacgcctg cttcgcgtac gggtggatgc ggccgtcgaa gttgcgattg
     1741 cccgagagga cggcggtcgc gtacaggtcg cggtcgacga tctcctgctg gatcttcggg
     1801 tcgagcgcgc cggacatgcc gttgcaggtc gtgcaggcga acgcgacgat gccgaagccg
     1861 agttgctcga gctcggactt gaggcccgcc tcctcgagat acagcgccac ggtcttcgat
     1921 cccggcgcga gcgacgactt cacccagggc ttgcgcttga gcccgaggcg gttcgcgttc
     1981 ctcgcaagca ggcccgcggc gatcacgttg cgcgggttcg aggtgttggt gcagctcgtg
     2041 atcgcggcaa tgatcaccgc gccgtccggc atctgaccgg gcacctcttc ccacgagccc
     2101 gcgatgccct tcgcggcgag atcggccgtt gcgacacggg cgtgtgggtt cgacgggccg
     2161 gccatgttgc gcacgaccga cgacaggtcg aactcgagcg tgcgctcgta ctcggcctgc
     2221 tgcaggctgt cggcccacag tccggcggtc ttcgcatagg tctcgacgag cttgacctgc
     2281 tcgtccgatc gccccgtgag cctgaggtac tggagcgtct gcgagtcgat cgcaaacatc
     2341 gccgcggtcg cgccgtactc cggcgccatg ttggagatgg ttgcgcggtc gccgagcgtg
     2401 agcgatgcgg cgccctcgcc gcggaattcc aggtacgcgc cgaccacctt cgacttgcgc
     2461 aggaactcgg tgagcgcgag gacgatatcg gtggcggtga tgccgggctg ccgccggccc
     2521 ttgagctcga cgccgacgat ctccggcagg cgcatccacg aggcgcggcc gagcatcacg
     2581 ttctcggcct cgaggccacc gacgccgacg gcgatcacgc cgagcgcgtc gacgtggggc
     2641 gtgtggctgt ccgtaccgac gcacgtgtcc ggaaacgcca cgccgtcctg cgcgtggatg
     2701 accggcgaca tcttctccag gttgatctgg tgcatgatgc cgttgccggc cgggatcacg
     2761 tcgacgttgt cgaacgcgag cttcgtccag tcgatgaagt ggaagcggtc ctcgttgcgc
     2821 cggtcctcga tggcgcgatt cttcgcaaac gcgtccgggt cgtaaccgcc gcactccacc
     2881 gcgagcgagt ggtccacgat cagctgcacc ggcaccaccg gattcacctt ggcgggatca
     2941 cccccacgtg ccgcgattgc gtcacgcagg ccggcgagat ccaccagtgc cgtctgaccg
     3001 aggatgtcgt ggcacaccac gcgcgcggga aaccacggaa aatcgcgctc gcgcttgcgt
     3061 tcgacgatct gccggaggca ctccgtcacg atcgccgggt ctgcgcggcg gacgacgttc
     3121 tccgcgtgca cgcgcgcggt gtacggcaac gtctcccagg cgccgggccg gatcgcctcg
     3181 accgcttccc gcgcgtcgta gtagtcgagc gcagtgccgg gcaatggctt gcggtgggcg
     3241 ctgttcatct cacttccgct tcgcgatcgg cacgaacttc cgatcttcgg ggccggtgta
     3301 gttcgcgctc ggacggatga tcttgccgtc gatgcgctgc tcgatcacgt gcgcggccca
     3361 gcccgtggtg cgcgagatga cgaacaacgg cgtgaacatc gcggtcggca cccccatcat
     3421 gtggtaggag accgcgctga accagtcgag gttcgggaac atcttcttga tgtcccacat
     3481 cacggactcg agtcgctcgg cgatgtcgaa catcttcatg tcgcccgctt ccttcgacag
     3541 cccgcgcgcg acttccttga tcaccttgtt gcgcgggtcg ccgatcgtat agaccgggtg
     3601 gccgaacccg atgatgactt ccttcgcctc gacgcgacgg cggatgtcgg cttccgcctc
     3661 gtcgggattc tcgtagcgct tctgcacctc gaaggcgacc tcgttcgcac cgccgtgctt
     3721 cggtccgcgc agcgcgccga tcgcgccggt gaccgctgag tacatgtcgg agccggtgcc
     3781 cgcgatcacg cgtgcggcga acgtggacgc attgaactcg tgctcggcgt agaggttcag
     3841 cgacacgtgc atcgccttga cccacgactc aggcggctgg cgacggtgca gcaggtgcag
     3901 gaagtgcccg cccaccgagt cgtcctctgt ctcgacgttg atcctctggc cggaatggct
     3961 gtagtggtac cagtagcaga gca
//
LOCUS       DAOMFD010000183         3969 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10335_length_3969_cov_35.346960, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000183 DAOMFD010000000
VERSION     DAOMFD010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3969)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3969
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10335_length_3969_cov_35.346960"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..492)
                     /locus_tag="PL152_09205"
     CDS             complement(55..492)
                     /locus_tag="PL152_09205"
                     /inference="COORDINATES: protein motif:HMM:NF022479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2721 domain-containing protein"
                     /protein_id="HQR49504.1"
                     /translation="MNGIQVVDIAHAIQLALAPVFLLSGIGVMLGVLTNRLARIVDRA
                     RKVEDSLRQATAPDIPEARNQLRVASRRARFINVSITLCTIAALLIALVVALVFSSTF
                     APIELGVYVATLFVLAMIALVGALLSFLVEVRIATAALRIGVK"
     gene            568..1308
                     /locus_tag="PL152_09210"
     CDS             568..1308
                     /locus_tag="PL152_09210"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HQR49505.1"
                     /translation="MSFKDHFSRQAAAYSRYRPGYPAELVEWVSSQAPDRRLALDCAT
                     GSGQAAVTLAVHFEAVVGIDGSLNQLRNAVAHPKVAYVAATAERMPVRDRTVSLVAAA
                     QAVHWFDFERFHAECRRVLVPGGAVAVWTYEKFRLDSLVDAVVDDFYDGTVGPFWPPE
                     RRYVEQAYRTLPFPWTEAAVPAFELVTEWDLDQVLGYLASWSAVQRYKDRNGVDPLPS
                     LRPQLEAAWGGTGRRRLHWPIHLRLGRA"
     gene            1380..3491
                     /locus_tag="PL152_09215"
     CDS             1380..3491
                     /locus_tag="PL152_09215"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF017464.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family metallopeptidase"
                     /protein_id="HQR49506.1"
                     /translation="MRWTMPVACLLTLTACAGNPPAQAPAASTPPVATPAAAPAKPQL
                     ASGVILANFDRNVRPQDDFYRYVNGGWLERTQIPPDRSNYGTFTMLADEVEKNLKTIV
                     EQAAATPGTSGSDSQMIGDLYASYMDEATAERNGPAALQAELAAIDAIKDRAGLVEYF
                     GRTQLRYVNAPPGIENFGAAAPLAATVFADAKHPDVNALYVDQFGLGMPDRDYYLKKE
                     GRFAEVRGKYHDYARELLELGGRKDAARSAARVVALETRLATAQWAKVELRDPNKVYN
                     PMDLAAAAKLTPGFDWSRWMAGAGIQGTGKLIVGEPSYFKAMAAAVREVPLETWKDYL
                     RVHVTDDYAALMGGKYEQARFAFRETTLTGVQEMRPRWKRGIQQVEFVMGQMLGRVYV
                     GQYFPPESKAQMQMLVSNLLTTFGESIDQLAWMSGGTKLAAHQKLAKFTVKIGYPDKW
                     PPKPEATIRRDDLVGNVMRTREGDRRIEFAKIGQPVDRTEWTMTPQTVNAYYNPLANE
                     IVFPAAILQPPFFDPKADNAVNYGAIVGVIGHEVSHGFDDEGRKFDGDGVLRDWWTAD
                     DNAKFQEKAHRLSGQYSAFSPIPGMNVNGDLTLGENIGDLSGLAMAYKAYHRSLAGSE
                     APVLDGYTGDQRFYIGWAQVWARKYREDELRKRLLTDPHSPSEYRVNGIVRNMEQFDR
                     AFDVKPGDKLYLPADEMVRIW"
     gene            3576..>3969
                     /locus_tag="PL152_09220"
     CDS             3576..>3969
                     /locus_tag="PL152_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005795319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HQR49507.1"
                     /translation="MRVLKIAAIAAASLLLSACGYNTFQTQDEQVKSAWSEVINQYQR
                     RADLIPNLVNTVKGFAAQEQAVLIGVTEARAKATQIKVDANDAGSLQQFQAAQGELSS
                     ALSRLMVVVEKYPELKSDQNFRDLQAQLE"
BASE COUNT          676 a         1363 c         1343 g          587 t
ORIGIN      
        1 agtccgctac tcgaccccgg gccccaaacc cggggtcttt tttttcgccg gcgctcactt
       61 gaccccgatc ctgagcgccg cggtcgcgat gcgcacctcg accaggaagc tgagcagcgc
      121 cccgaccagc gcgatcatgg ccagcacgaa cagcgtcgcg acgtacacgc ccagctcgat
      181 cggcgcgaag gtgctcgaga acaccagtgc gaccaccagc gctatgagca gcgcggcgat
      241 cgtgcagagc gtgatcgaga cgttgatgaa gcgcgcgcgt cgcgacgcca cccgcagctg
      301 gttccgtgcc tcggggatgt ccggcgcggt cgcctgccgc aggctgtcct cgaccttgcg
      361 ggcgcggtcc acgatgcgag cgaggcgatt ggtgagcacg ccgagcatca cgccgatgcc
      421 ggacagcagg aacacgggtg cgagcgcgag ctggatcgcg tgcgcgatgt cgacgacctg
      481 gatcccgttc atgcggcgat tctagtccag gcgaccgggc cggttgcggc tccgcgacgg
      541 gtcccgcacc atgcggagcg caccgccatg tccttcaagg atcatttctc caggcaggct
      601 gccgcgtaca gccgctaccg gccgggctat ccggccgaac tggtcgagtg ggtgtcgtcc
      661 caggcacccg accgccggct cgccctcgac tgcgcgaccg gcagcggaca ggccgcggtc
      721 acgctcgccg tgcacttcga ggcggtcgtc gggatcgacg ggagcctgaa tcagttgcgc
      781 aacgccgtcg cgcacccgaa ggtcgcgtac gtggctgcca cggcggagcg catgccggtc
      841 cgggaccgga cggtttcgct cgtcgccgcg gcacaggccg tccactggtt cgacttcgag
      901 cgctttcacg cggaatgccg ccgcgtgctg gtgccgggcg gcgcggtggc cgtgtggacc
      961 tacgagaagt tccggctgga ttcgctcgtc gatgcggtcg tggatgactt ctacgatggg
     1021 accgtgggcc cgttctggcc gccggagcga cggtacgtgg agcaagcgta ccgaacgctg
     1081 ccttttccct ggacggaggc cgcggtcccg gccttcgaac tcgtgacgga gtgggacctc
     1141 gaccaggtgc tcggctacct ggcgtcgtgg tcggcagtgc agcgctacaa ggaccgcaac
     1201 ggggtcgatc ccctgccttc gctgcgtccg cagctcgaag cggcgtgggg cgggaccggc
     1261 cggcgccgcc tgcactggcc gatacacctg cggctcggcc gggcctgatt cctctatact
     1321 tcgcgccctt ccgtcggcgc ccgatggtgg cgcccggtcc cgacccggag acgacaacca
     1381 tgcgctggac gatgcccgtg gcctgcctgc tgaccctgac tgcctgcgcc ggcaatccgc
     1441 cggcgcaagc gcctgctgcg tcgacgccgc cggtcgcgac gcccgctgcc gctcccgcga
     1501 agccccagct cgcgtcgggc gtcatcctcg cgaacttcga ccggaacgtg cgtccgcagg
     1561 acgacttcta ccggtacgtg aacggcggct ggctcgagcg gacccagatc ccgcccgatc
     1621 gctcgaacta cggcacgttc accatgctgg ccgacgaggt cgagaagaac ctgaagacca
     1681 tcgtcgagca ggcggcggcg acgcccggca cttcgggcag cgactcgcag atgatcggcg
     1741 acctctacgc gagctacatg gacgaggcga ccgccgagcg caatggaccc gcggcgctgc
     1801 aggccgagct ggccgcgatc gacgcgatca aggatcgcgc ggggctcgtc gagtacttcg
     1861 gccgtaccca gctgcgctac gtcaacgcgc ctccgggcat cgagaacttc ggcgccgccg
     1921 cgccgctggc cgcgaccgtc tttgccgacg ccaagcaccc cgacgtcaat gctttgtacg
     1981 tcgaccagtt cggcctcggc atgccggacc gcgactacta cctgaagaag gagggtcgct
     2041 tcgcggaagt ccgcggcaag taccacgact acgcgcgcga gttgctcgag ctcggcggac
     2101 gcaaggacgc tgcgcggtcg gcggcacggg tcgtggcgct cgagacgaga ctcgccacgg
     2161 cacagtgggc gaaagtagag ctgcgcgacc cgaacaaggt ctacaacccc atggacctcg
     2221 ccgccgcggc gaagctcaca ccgggcttcg actggtcgcg ctggatggcg ggggccggca
     2281 tccagggcac gggcaagctc atcgtcggcg agccgagcta cttcaaggcc atggcggctg
     2341 ccgtccgcga ggtgccgctc gagacgtgga aggactacct gcgggtgcac gtcaccgacg
     2401 actacgccgc gctgatgggc ggcaagtacg agcaggcgcg gttcgccttc cgggagacga
     2461 cgctcaccgg tgtgcaggag atgcggccac ggtggaagcg cggcatccag caggtcgagt
     2521 tcgtgatggg gcagatgctg ggccgcgtct acgtcgggca gtacttcccg ccggaatcga
     2581 aggcgcagat gcagatgctg gtgtcgaatc tcctcacgac gttcggcgag tcgatcgacc
     2641 agctggcctg gatgagcggc ggaaccaagc ttgcagccca ccagaagctg gcgaaattca
     2701 cggtcaagat cggctatccc gacaagtggc cgccgaagcc ggaggcgacg atccgccgcg
     2761 acgacctcgt cggcaacgtc atgcgcaccc gcgaaggcga ccgccgcatc gagttcgcga
     2821 agatcggcca gccggtcgac cggaccgagt ggaccatgac gccgcagacc gtcaacgctt
     2881 actacaaccc gctggccaac gagatcgtgt tccccgcggc gatcctgcag ccgccgttct
     2941 tcgaccccaa ggcggacaac gccgtgaact acggcgccat cgtcggcgtg atcggccacg
     3001 aggtgagcca cggcttcgac gacgagggcc gcaagttcga cggcgacggc gtgctgcgcg
     3061 actggtggac tgccgacgac aacgccaagt tccaggagaa ggcccaccgg ctcagcgggc
     3121 agtactccgc gttcagcccg atccccggca tgaacgtcaa tggcgacctg acgctgggcg
     3181 agaacatcgg tgacctgtcc gggctggcga tggcgtacaa ggcctaccac cgttcgctgg
     3241 ccggctcgga ggccccggtg ctggacggtt acaccggcga ccagcgcttc tacatcggct
     3301 gggcccaggt ctgggcccgc aagtaccgcg aggacgagct gcgcaagcgc ctgctcaccg
     3361 acccgcacag cccgtcggag taccgggtca acgggatcgt gcgcaacatg gagcagttcg
     3421 accgcgcctt cgacgtcaag ccgggcgaca agctctacct gccggccgac gaaatggtcc
     3481 ggatctggtg aacgaccggt catggatatt tcgccgctgc tccccataat tgactgcact
     3541 cggctggagc ccccggcctg tgaggaggaa cagtcatgcg cgtcttgaag atcgccgcga
     3601 ttgccgccgc gagcctgctg ctctcggcct gcggctacaa cacgttccag actcaggacg
     3661 agcaggtgaa gtcggcctgg tccgaggtga tcaaccagta ccagcgccgc gccgacctga
     3721 tccccaacct cgtcaacacc gtgaagggct tcgccgccca ggagcaggcc gtgctgatcg
     3781 gcgtcaccga ggcgcgtgcc aaggccaccc agatcaaggt ggatgcgaac gacgccggct
     3841 cgctccagca gttccaggcg gcgcaaggcg agctgtcgag cgcgctgtcc cggctcatgg
     3901 tcgtggtcga gaagtatccc gagctcaagt cggaccagaa cttccgcgac ctgcaggcgc
     3961 agctcgagg
//
LOCUS       DAOMFD010000184         3948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10439_length_3948_cov_32.741331, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000184 DAOMFD010000000
VERSION     DAOMFD010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3948
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10439_length_3948_cov_32.741331"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..432
                     /locus_tag="PL152_09225"
     CDS             <1..432
                     /locus_tag="PL152_09225"
                     /inference="COORDINATES: protein motif:HMM:NF015436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HQR49508.1"
                     /translation="LNFQRQRRRVAFVVDEYGDIQGLVTIEDLLEEIVGDFTTDTSAL
                     HKDVHKERGGTFVVNASASVRTLNRKMGWTLPTTGPRTLNGLIIEYLETIPDAGTSLR
                     IDNYSIDVLQTGDNAVKTVRLKPIVSPASAAAADGSRPGHA"
     gene            complement(465..1817)
                     /gene="radA"
                     /locus_tag="PL152_09230"
     CDS             complement(465..1817)
                     /gene="radA"
                     /locus_tag="PL152_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HQR49509.1"
                     /translation="MARSRTSYVCGSCGAATPQWQGQCPACQAWNTLELAASSIASPA
                     ARRGTAAAATSLAVSEVGDDSELRAATGIGELDRVLGGGLVAGSVVLLGGDPGIGKST
                     LLLQAADALSRRQPVLYASGEESVRQVALRARRLALESPSLRLAAETAVERIITEAAA
                     AQARVLVIDSIQTMHTELSESSAGSAVQVRESAARLVRYAKESATAVLIIGHVTKEGL
                     IAGPRILEHMVDTVLYFESDPGSRFRVVRAVKNRYGAANEIGVFAMVDQGLKEVRNPS
                     AIFLSRHDTPVAGSVITVAREGSRPLLVEVQALLDDAQGASPRRVAVGFESSRLALLL
                     AVLHRHGGISTSGQDVFVNVVGGVRIGETAADLPAVLAVHSSLRNRAVASDLVCFGEL
                     GLTGEIRPVPFGEERLREAAKQGFRRALVPAANVPRRAPEGLVVQGAARLDEALAAAD
                     "
     gene            1948..2859
                     /locus_tag="PL152_09235"
     CDS             1948..2859
                     /locus_tag="PL152_09235"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQR49510.1"
                     /translation="MRDPADETQIVSSTGGRPRPQATRRAVPERLGRYEIIDELGKGA
                     MGVVYRARDPRINREVALKAIPLAAEFEGDELEEARTKFFREAEMAGRLSHPHIVTIH
                     DAGEDHGTAYIAMELLRGRHLVDYTASSQLLPVNVAIELLARLADALHYAHQQHVVHR
                     DIKPANVMYDPASGELKITDFGIARLTDSNRTRTGVVLGTPSFMSPEQLQGRAVTGRS
                     DQFSLAITACQLLTGQLPFRADSMPGLMVKIAHEPHPRIRALRPDLPEGIDAVFDRAL
                     AKDPADRYDSCGAFATALRDLVAGGEA"
     gene            2862..3686
                     /locus_tag="PL152_09240"
     CDS             2862..3686
                     /locus_tag="PL152_09240"
                     /inference="COORDINATES: protein motif:HMM:NF033484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HQR49511.1"
                     /translation="MHLKGKFRCVGVSDTGRIREHNEDTIGTDADIGLVVLADGMGGY
                     KSGEVASGIAVRTVMTLLKDAVDREDLSIRDKESGLSRPGILLRDAIHRANKIIYQTA
                     KTQPNCEGMGTTVVAGLFYDDNLTIAHVGDSRLYCHRGGELRQVTQDHSLLQELVSRG
                     FYTREEAQRAAAKNYVTRALGVEPSVDVDVTEFPVIKDDLFLLCSDGLSDMVEDDDIH
                     LTISTFGGNLETLAKQLVQLSNDNGGRDNVSIVVVRVLDSFPARRGIFDRLLARFG"
     gene            3708..>3948
                     /locus_tag="PL152_09245"
     CDS             3708..>3948
                     /locus_tag="PL152_09245"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HQR49512.1"
                     /translation="MARLVLSLDGQSLAEYNMSKERYTIGRLPDNDIRIDNPAVSGHH
                     ALIINILNDSFLEDLNSTNGTYVNGKIIKKHALQHG"
BASE COUNT          671 a         1407 c         1308 g          562 t
ORIGIN      
        1 ctgaacttcc agcgccagcg gcgtcgcgtg gcgttcgtcg tcgacgagta cggcgacatc
       61 cagggcctcg tcaccatcga ggacctgctc gaggagatcg tcggcgattt cacgaccgac
      121 acgagcgcgt tgcacaagga cgtccacaag gaacggggcg gcacgttcgt ggtgaacgcg
      181 tccgcgagcg tccggaccct gaatcgcaag atggggtgga ctctgccgac gaccggcccg
      241 cgcacgctga acggcctgat catcgagtac ctcgagacca tcccggacgc gggcacgagc
      301 ctgcgcatcg acaactactc gatcgacgtg ctgcagaccg gcgacaacgc ggtgaagacg
      361 gtgcggttga aacccatcgt ctcgcctgcg tcggctgccg cagccgacgg cagccggccc
      421 ggacacgcct gaaccggccg gcggcgtggc cgattgccgg gcgctcagtc ggcggccgcg
      481 agggcctcgt ccagtctcgc cgcaccctgc acgaccagcc cttccggcgc acgccgcggt
      541 acgttcgccg cgggcacgag ggcgcgacgg aacccctgct tggccgcctc gcgcaggcgc
      601 tcctcgccga acggcaccgg ccggatctcg cccgtgagtc cgagctcacc gaagcagacc
      661 aggtcgctcg caaccgcacg gttgcgcagg ctcgagtgca ccgcgagcac cgccgggagg
      721 tccgcggcgg tctcgccgat gcgcacgccg ccgacgacgt tcacgaagac gtcctggccg
      781 gacgtcgaaa tgcccccgtg ccggtgcagg accgcgagca gcagcgcaag gcgactcgac
      841 tcgaacccga cggccacccg gcgcggcgag gcgccctgcg cgtcgtcgag cagcgcctgc
      901 acttcgacga gcagcggccg gctgccctcg cgcgcaacgg tgatcacgct gccggccacg
      961 ggcgtgtcgt gccgtgacag gaagatggcg gacgggttgc ggacctcctt caggccctgg
     1021 tccaccatgg cgaacacgcc gatctcgttg gcggcgccgt agcggttctt gaccgcccgg
     1081 acgacccgga accggctccc ggggtcgctc tcgaagtaca gcaccgtgtc caccatgtgc
     1141 tcgaggatgc gcggtccggc gatcagccct tccttggtga cgtggccgat gatgagcacg
     1201 gcggttgccg attccttcgc gtagcgcacc aggcgcgccg cggactcgcg cacctgcacc
     1261 gccgatccag cggaggattc cgacaattcg gtatgcatgg tctggatcga gtctatgacc
     1321 agcacccggg cctgcgccgc ggcggcctcg gtgatgatgc gctcgacggc ggtctcggcg
     1381 gcgagcctga gggatggcga ctcgagggcc agccgccgcg ccctcagcgc cacctggcgc
     1441 acggattcct cgcccgacgc gtagagcacc ggctggcggc gcgagagcgc gtccgctgcc
     1501 tgcagcagca aagtggactt gccgatgccc gggtcaccgc cgagcaggac gaccgagccc
     1561 gccacgagcc ctcctcccag cacgcggtcc agttcaccga tgccggtggc ggcgcgcagt
     1621 tccgaatcgt cgcccacttc ggatactgca aggctggtcg cggccgcggc ggtcccgcgc
     1681 ctcgcggccg gggaggcgat cgacgaagcc gcgagttcca gggtgttcca ggcctggcac
     1741 gcgggacact gcccctgcca ctgcggcgtc gccgcgccgc aggagccgca cacgtagctc
     1801 gtccgggatc gtgccatgat gcaaccctgc ccccgggaca ccccgcccgg agggtccgcg
     1861 atgctagcac gctcggccgg gcgccccgga tgtgtgacgg cgctggcaaa tcgcggcgga
     1921 ccccctacgt atacttggcc cacaaccatg cgcgacccgg ccgacgagac ccagatcgtg
     1981 tccagcaccg gcggccgccc caggccgcag gcgacccgtc gcgccgtgcc cgaacggctc
     2041 ggccgctacg agatcatcga cgagctcggc aagggcgcga tgggcgtcgt ctatcgcgcc
     2101 cgcgacccgc gcatcaatcg cgaggtggcc ctcaaggcga ttccgctggc tgcggaattc
     2161 gaaggcgacg agctcgagga agcccgcacc aagttctttc gcgaagcgga aatggccggc
     2221 cggctgagcc acccccacat cgtcacgatc cacgacgccg gcgaggacca cggcaccgcc
     2281 tacatcgcga tggagttgct ccgcggacgg cacctcgtcg actacaccgc atcgtcgcag
     2341 ctgctgccgg tgaacgtcgc gatcgaactg ttggcgcggc tggccgacgc gctgcattat
     2401 gcgcaccagc agcacgtggt gcaccgcgac atcaagccgg ccaacgtcat gtacgacccc
     2461 gcgagcggcg agctcaagat caccgacttc ggcatcgccc gcctcaccga cagcaaccgg
     2521 acccgcaccg gcgtcgtcct ggggactcct tccttcatgt ccccggagca actgcaggga
     2581 cgtgccgtga ccgggcgatc agaccagttc tccctggcca tcaccgcctg ccagctgctg
     2641 accgggcagc tgccgttccg cgcggactcg atgcccgggc tgatggtgaa gatcgcccac
     2701 gagccgcacc cgcggatccg ggccctgcgc cccgacctgc ccgaggggat cgacgcggtg
     2761 ttcgatcgcg cgcttgcgaa ggacccggcg gaccgctacg acagctgcgg cgcgtttgcc
     2821 acggcgctgc gcgacctggt cgccggcggc gaggcctgac cgtgcacctc aagggcaagt
     2881 tccgctgcgt cggggtgagc gacaccggtc gcattcgcga gcacaacgag gacacgatcg
     2941 gcaccgacgc ggacatcggg ctcgtcgtcc tcgccgacgg catgggtggc tacaagtccg
     3001 gcgaagtcgc cagcggcatc gccgtgcgca ccgtgatgac gctgctcaag gacgccgtcg
     3061 accgcgagga cctgtcgatc agggacaagg agtccgggct gtcccggccg ggcatcctgc
     3121 tgcgcgacgc gatccaccgc gccaacaaga tcatctacca gaccgcgaag acacagccca
     3181 attgcgaggg catgggcacg accgtcgttg cggggctctt ctacgacgac aatctcacca
     3241 tcgcgcacgt cggcgactcg cggctgtact gccaccgcgg gggtgaactg cggcaggtca
     3301 cgcaggacca ctcgctgctg caggaactgg tctcgcgggg tttctacacc cgcgaggagg
     3361 cccagcgcgc ggccgccaag aactatgtca cgcgggcgct gggggtcgag ccgtcggtcg
     3421 acgtggatgt gaccgagttc ccggttatca aggacgacct gttcctgctc tgctcggacg
     3481 gtctttccga catggtcgag gacgacgaca ttcatttaac gatcagtacc tttggtggta
     3541 atcttgagac gctcgccaag caactcgtgc agttgagcaa cgacaatggc gggcgggaca
     3601 acgtgtcgat cgtggtggtc cgggtgctcg acagcttccc ggcccggcgc gggatcttcg
     3661 acaggctgct cgcccgcttc ggttagttgg gaggccctgg gacacacatg gcaaggcttg
     3721 ttctcagcct cgacgggcag tcgctcgccg agtacaacat gagcaaagag cgctacacga
     3781 tcgggcgcct tccggacaac gacatccgca tcgacaatcc ggcggtgagc ggccaccacg
     3841 cgctgatcat caacatcctc aacgactcgt tcctcgagga cctgaacagc accaacggca
     3901 cgtacgtcaa cggcaagatc atcaagaagc acgcgctgca gcacggcg
//
LOCUS       DAOMFD010000185         3918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10571_length_3918_cov_25.207870, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000185 DAOMFD010000000
VERSION     DAOMFD010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3918
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10571_length_3918_cov_25.207870"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1787)
                     /locus_tag="PL152_09250"
     CDS             complement(<1..1787)
                     /locus_tag="PL152_09250"
                     /inference="COORDINATES: protein motif:HMM:NF013714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR49513.1"
                     /translation="MANSVINDVTGLNPIGVAGVVTPRTVDDVVQALRSTRGPVSIGG
                     GRFSMGGQTASTGAMQIDMRGMNRVLTFYPADRRIKVEAGIRWCDIQRFVDPHGLAVK
                     IMQTYANFTVGGSLSVNVHGRYMGLGPLILSVRWLQLVLASGETVECSPAMNPDLFYG
                     AIGGYGAVGVIVAAELDLADNVRVKRDSRRMPVAQYLEYFKNHVRADRQALFHNADLY
                     PPHYRRVNAVTWRETLDRPTSARLQSPGGLYPLHKYLLWAITETPFGRWRREHLVDPI
                     VFAGRPVHWRNFEAGYDVAELEPLSRRHSTYVLQEYFVPLDRFEAFTLRLGEILARHK
                     VNVINVSVRHALADPGSVMAWARTECFAYVLYYKQRRRPSAIERVGVWTRELIEAVLE
                     CGGTYYLPYQLHATPEQFRRAYPAAERLFDIKRRIDPDARLRNALWDKYCPAATQPSA
                     APAPANDFQAVYGHTKWRDDFYRFLQNVFNVYPEDRFHWLIAEAVAEGGDEEAIYRRI
                     LTRRSQAAAFLNPLRYALPSLFRQKAEMKRQTLGLLNGARAFSGYAEVGSFGRYASVL
                     RSDLSLAQITLIDDKPPDNSPVQILERGG"
     gene            complement(1790..2263)
                     /locus_tag="PL152_09255"
     CDS             complement(1790..2263)
                     /locus_tag="PL152_09255"
                     /inference="COORDINATES: protein motif:HMM:NF017470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HQR49514.1"
                     /translation="MQSVAAVAQGLTMAGKVNRLRPGLHRRESSIAIDRNAVVQAALS
                     WALPRGRIARGRFVVSFAILLALGYVLVQGLDATLGEMAGTVVVALLLWLAAMLSARR
                     LHDTGRSAWWLMVFAVPVLGALWLAWVLLLQRGTQGDNAFGADPTQRAADYLTVA"
     gene            2300..3853
                     /locus_tag="PL152_09260"
     CDS             2300..3853
                     /locus_tag="PL152_09260"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D family protein"
                     /protein_id="HQR49515.1"
                     /translation="MTPLVSSQLDMHSLPITGLKVRLRIVVLACALALIGPSGAVGAT
                     ADMAFVDAEVAVRAGQSGVYVLDSGEEALLARAWLADQARESIEVQYFIWSTDNIGIL
                     AAEALLRAADRGVRVRVIVDDLLIDAPDKSLLALAKHPRIDIRIYNPKTSVGVPLLTR
                     LLNVVLGFRDVNQRMHDKTVVVDGRVAITGGRNMAAEYFDYNHSYNFRDRDVLLLGAV
                     VADVRASFERFWSSGLAVPVEALYDGLGLMQKHVVVQDEDVQQVYTDLRDYARLPSNF
                     APEVRAAIAATPTAFPRIARHVAWGRVEFISDRPGKNDSRVSLAGGGETTRRLAALVD
                     AAQESIIIQSPYLVLSDAAIELFKRARARGVRVAVSTNSMASTDNLPAFSGYRSQRQR
                     LLAMGIEIFEYRPDAAVQRASMRPYLAAATKPPIFSMHAKSLVVDSIVAFVGTFNLDP
                     RSENLNTEVGVVIFDPLVAKAVEASILADMAPGSSWSAASDNPDQYAPLSKRAKVRLL
                     ELLPLRQLL"
BASE COUNT          684 a         1284 c         1290 g          660 t
ORIGIN      
        1 aacccgccgc gttcgaggat ctgcaccggg gagttgtccg gcggcttgtc atcgatcagc
       61 gtgatctgcg cgagcgagag gtcactgcgc agcactgacg cgtagcggcc gaaggagccg
      121 acttcggcgt agccactgaa tgccctggct ccgtttagca gcccgagcgt ctgccgcttc
      181 atctccgcct tctggcggaa cagggacggc agcgcatacc gcagtggatt caggaaggcg
      241 gcggcctggc tacggcgcgt caggatgcgg cggtagatcg cctcctcgtc gccgccctcg
      301 gcaacagcct cggcgatgag ccagtggaac cggtcctcgg ggtagacatt gaagacgttc
      361 tgcaggaagc ggtagaagtc atccctccac ttcgtgtgcc catagaccgc ctggaagtcg
      421 ttcgccggcg cgggcgcagc ggagggctgg gtggctgccg ggcagtattt gtcccacagc
      481 gcgttgcgca gacgggcgtc cggatcgatt cgacgcttaa tgtcgaacag ccgctcggcc
      541 gcggggtacg cgcggcggaa ctgctcgggc gtcgcatgca actggtacgg aaggtaataa
      601 gttcccccgc actcgaggac cgcctcgata agttcgcgtg tccagacgcc gacgcgctcg
      661 atcgcgctcg gacggcggcg ctgcttgtaa tagagcacgt aggcaaagca ctcggtgcgc
      721 gcccaagcca tgaccgagcc cgggtcagcc aaggcgtggc gcaccgagac attgatgacg
      781 ttgaccttgt gtcgcgcgag gatctccccg aggcgcagcg tgaaggcctc gaagcgatcc
      841 agggggacga agtactcctg cagcacatag gtcgaatgcc gtcgcgagag cggttcgagc
      901 tctgccacgt cgtaaccggc ctcgaagttg cgccagtgca ccggtcggcc ggcgaagacg
      961 atcggatcca ccaggtgctc gcggcgccac cggccgaacg gcgtctcggt gatggcccac
     1021 agcaggtact tgtgcagcgg atacaggccc ccaggcgact gcaggcgcgc gctcgtcggc
     1081 cggtccaagg tctcccgcca ggtgacggca ttgacgcgcc gatagtgcgg cggatagagg
     1141 tcggcgttgt ggaacagcgc ctggcggtcg gcgcggacgt ggtttttgaa gtactccagg
     1201 tactgcgcca ccggcatacg gcgggagtcg cgcttgacgc ggacgttatc ggcgaggtcg
     1261 agttccgcgg ccacgatgac gccgacggca ccgtagcctc cgatcgcgcc gtagaagaga
     1321 tcagggttca tcgccggtga gcactcgacc gtttccccgg aagccagtac gagctgtagc
     1381 cagcgcaccg agagaatgag cggtcccagg cccatgtagc ggccgtggac gttgacgctg
     1441 agggacccgc ccacagtgaa gttggcgtag gtctgcatga tcttcacggc caggccgtgg
     1501 ggatcgacaa aacgctggat gtcgcaccag cggatgcccg cctcgacctt gatccggcga
     1561 tccgccgggt agaacgtcag cacgcgattc atgccccgca tgtcgatctg catcgcgccc
     1621 gtgctggcgg tctggccacc catgctgaag cgcccgccgc cgatcgatac gggtccgcgt
     1681 gtcgagcgca gcgcctggac gacatcgtcg accgtacgcg gcgtgacgac gccggccacg
     1741 ccgatcgggt tcagtccagt gacgtcgttg atgaccgagt tcgccatggt cacgccaccg
     1801 tcagatagtc ggccgcgcgc tgtgtcgggt ccgcaccaaa cgcgttgtca ccctgggtgc
     1861 ctcgctgcag aagcagcacc caagctagcc atagggctcc gagcaccggc accgcaaaga
     1921 ccatcagcca ccacgccgag cggcccgtat cgtgcagcct ccgggccgag agcatcgcgg
     1981 ccagccagag cagaagcgcg acgacgaccg tgcccgccat ttcccccagc gtggcgtcga
     2041 gcccctggac gagcacgtag cccagtgcga gcaggatcgc aaacgaaacc acgaaccgtc
     2101 cccgtgcgat gcgcccgcgc ggcagggccc agctaagcgc cgcttggacg actgcgtttc
     2161 ggtcgatggc gatggaagac tcccggcgat gcaggccggg ccgaagtcta tttactttgc
     2221 ccgccatcgt cagcccttgc gcaacagcgg cgacggactg catttggtgc gctgcactgg
     2281 aggggatgcg cggacacggg tgactccgtt ggtatcgtcg caactcgata tgcactcact
     2341 gccaatcacc ggtcttaagg tgcgccttcg catcgtcgtc ctagcgtgcg cgctcgcgct
     2401 catcgggcca tcgggcgccg tcggcgcgac ggccgatatg gcgttcgtcg acgcggaggt
     2461 cgccgtccgt gcagggcaaa gcggcgtcta cgtgctcgac agcggcgaag aagcgctgct
     2521 cgcccgcgcc tggctcgccg atcaggcgag agagtcgatc gaggtccagt acttcatctg
     2581 gagcaccgac aacatcggca tcctcgccgc tgaggcgctg ctgcgcgccg ccgaccgcgg
     2641 cgtgcgcgtt cgggtgatcg tcgacgattt gctgatcgac gctcccgaca agtcgctgct
     2701 ggcactggcc aagcacccgc gcatcgacat ccgcatctac aaccccaaga cctcggtcgg
     2761 cgtgccgctg cttacgcggc tgctgaacgt ggtcctgggt ttccgcgatg tcaatcagcg
     2821 aatgcacgac aagacggtgg tcgtcgacgg ccgtgtcgcc attaccggcg gccgcaacat
     2881 ggcggccgag tatttcgact acaaccactc ctacaacttc cgagaccgcg acgtcctgct
     2941 gctcggtgcc gtcgtcgccg acgtgcgggc cagcttcgaa cgcttctggt cgagcggact
     3001 cgcggtgccg gtcgaagcgc tgtacgacgg ccttggcctg atgcagaagc acgtggtggt
     3061 gcaggacgag gacgtccagc aggtctacac ggatctgcgc gactatgcgc gcttgccgtc
     3121 gaacttcgca cccgaggtcc gtgcggcgat cgccgccacc ccgaccgctt ttccgaggat
     3181 cgctcgccac gtggcctggg gtcgcgtcga gttcatcagc gaccggccgg gcaagaacga
     3241 cagtcgcgtc agtttggctg gcggtggtga gaccacccgg cgactcgccg cgctcgtgga
     3301 cgccgcgcag gagagcatca tcatccagtc gccatacctg gtcctgagcg acgccgcgat
     3361 cgaactcttc aagcgtgccc gcgcgcgcgg cgtacgggtg gccgtcagca ccaactcgat
     3421 ggcgtccaca gacaatctgc ctgcattcag cggttaccgc agccaacgcc agcgtctgct
     3481 ggcgatgggc attgagatct tcgagtaccg accggacgcg gccgttcagc gcgcgtcgat
     3541 gcgtccgtac ctcgcggcgg ccacgaaacc gccgatcttc tcgatgcacg cgaagtcctt
     3601 agtcgtggac tcgatcgtcg ccttcgtcgg cacgttcaat ctggacccgc gatccgagaa
     3661 cctgaacact gaagtcggcg tggtcatctt cgacccactt gtcgccaagg cggtggaggc
     3721 gtcaatcctg gctgacatgg cgccggggag cagttggagc gcggcatccg acaacccgga
     3781 tcaatatgcg ccactatcga agcgcgccaa ggtgaggttg ctggagctcc ttcccttgcg
     3841 gcaattgttg tagcgtgacc ctttgcccct tgtaaccgga ggagatcacg gctttcattc
     3901 aggggcacag gtcggcca
//
LOCUS       DAOMFD010000186         3891 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10712_length_3891_cov_33.266423, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000186 DAOMFD010000000
VERSION     DAOMFD010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3891)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3891
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10712_length_3891_cov_33.266423"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..646)
                     /locus_tag="PL152_09265"
     CDS             complement(<1..646)
                     /locus_tag="PL152_09265"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HQR49516.1"
                     /translation="MPYLYLVRHAQPDFAGHYDSVTSLGIRQSTWLGEHLALRGIEFE
                     RVISGSLARQAGTLDAILPALSHAPERSVDPRFNEYDAASVLSTFHLGDEQALRASGD
                     RRRYFLAVRDALTAWSRRDEAIAGGETWREFGERVHGAVATACEGLDTDDAVLVVTSG
                     GVIGRIVADTLAAGPDAAIQLNLQTRNTGITELACGRSARRVVAFNAVPHLERAD"
     gene            738..1553
                     /locus_tag="PL152_09270"
     CDS             738..1553
                     /locus_tag="PL152_09270"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HQR49517.1"
                     /translation="MTEPARQFRACGIVTLTTDFGLKDPFVGVMKGQLLTRLPGVRIV
                     DLTHDVLAHWPAEAGFWLSRSFRYFPAGTVHVAVVDPGVGSDREIAAVESEGHLFLAP
                     DNGLLGAVVDASRASTLRHVEAGVLAKLGIVARSATFHGRDIFAPLAAELASGRLQPA
                     ALGPAVPELIPGWIDEPTVSPGQVAGVVVTIDHFGNLLTNIDVALVRTLSHPCIRVAG
                     RELPLRRTYSDVRPGEYLGLVNSFGVLEIARAEMSAAEGLGLDRGAPVTVVQK"
     gene            1720..1926
                     /locus_tag="PL152_09275"
     CDS             1720..1926
                     /locus_tag="PL152_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49518.1"
                     /translation="MERDEEHFDLDEEFVANAEEEDYDRFIDRADRRKQAAVKRGKAA
                     WSKLEDVLAEKRLQKELKDVYDDV"
     gene            1985..>3891
                     /locus_tag="PL152_09280"
     CDS             1985..>3891
                     /locus_tag="PL152_09280"
                     /EC_number="2.7.2.4"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF006515.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/diaminopimelate
                     decarboxylase"
                     /protein_id="HQR49519.1"
                     /translation="MTSNTESHWVVLKFGGTSVSSVSNWRNVAAVVRARLDEGLRPIV
                     VHSALSGITDRLEQLLAKALTGDWEPVMEQIERRHVDLSRDLGIPLSAELEHHFNDLR
                     RMASGIHLIGEVSDRLRARVMATGELMATRIGAAYLASEKLDVQWLDARTVLHAEERA
                     GATVRANYLSATCSFEPDASLQRRLASGGSVLLTQGFIASDANGETVLLGRGGSDTSG
                     SYFAAKLHARRLEIWTDVPGMFTANPRAVPNARLLKSLDYDEAQEIASSGAKVLHPRC
                     ILPAKQYGIPLSVHATQQPDLEGTVITSTGGDGGARVKAVCTKKGITLISMETLGMWH
                     QVGFLADAFAVFKDHGLSVDLVSTSETTVTVSLDPAANALDTRALETLVNSLSKLCRV
                     EVIGPCSAVSLVGRSIRATLHRLGDALELFEEQRIYLVTQAANDLNITFVVDEEQGDR
                     LVSRLHDVIVRNAPSDHVLGPTWEQIHGIGTAHPVGGPPWWQGRRAELEKIARAQGSA
                     YVYDRDTMVRAAKQLLAIDGVERIFYAVKANPHPEVLRQFADLGLAFECVSQGECERV
                     IETIPGIARDRILFTPNFAPRSEYEWAIEAGVHLTIDNLHALRHWPELFRGRDVLVRV
                     DTGFGRGHHDHV"
BASE COUNT          627 a         1324 c         1343 g          597 t
ORIGIN      
        1 ggtccgcgcg ctcgaggtgc ggcacggcgt tgaacgcgac cacgcgccgc gccgagcgac
       61 cgcaggccag ttcggtgatg cccgtgttgc gcgtctgcag gttgagctgg atcgccgcgt
      121 cgggcccggc cgccagcgtg tcggcgacga tgcggccgat gacgcccccc gacgtcacca
      181 cgagcaccgc atcatcggtg tcgagtccct cgcacgccgt ggcgaccgcg ccgtgcacgc
      241 gttcgccgaa ttcgcgccac gtctcgccgc ccgcgatcgc ctcgtcgcgg cgcgaccagg
      301 ccgtcaaggc gtcacgaacc gcgaggaaat agcggcgccg gtcgcccgag gcacgcagtg
      361 cctgctcgtc gccgaggtga aatgtcgaca gcacgctcgc ggcgtcgtac tcgttgaagc
      421 gggggtcgac gctgcgctcc ggcgcatgtg acagggccgg caggatcgcg tcgagcgtgc
      481 ccgcctgtcg cgccaggctg cccgagatca cgcgctcgaa ctcgatgccg cgcaacgcca
      541 ggtgctcgcc cagccacgtg gactggcgga tgccgagcga cgtgaccgag tcgtagtgcc
      601 cggcgaagtc cggttgcgca tggcgcacga ggtacaggta gggcatgccg cggaatcggg
      661 acgcgtgact gggaggaggg acgcagtcta acctgtcgcc acgatcaggc atcggtgccg
      721 gaggcgcgac gagacgcatg accgaacccg ccaggcaatt tcgggcttgt ggcatcgtca
      781 cgctgacgac ggattttggg ctcaaggatc cgttcgtcgg cgtgatgaag gggcagttgc
      841 tcacgcgact tcccggcgtg cgcatcgtcg acctgacgca cgacgtgctc gcgcactggc
      901 ctgccgaggc cggcttctgg ctgtcgcggt cgtttcgtta cttccccgcc ggcacggtgc
      961 acgtggccgt ggtcgacccc ggcgtcggga gcgaccgcga gattgccgcg gtcgagtcgg
     1021 aagggcacct gttcctggcg ccggacaacg gcttgctggg cgcggtggtc gatgcgtcgc
     1081 gcgcgtccac gctgcgtcac gtggaagcgg gagtcctcgc gaagcttggc atcgttgccc
     1141 gaagcgcgac atttcacgga cgcgacatct tcgcgccgct cgcggccgag ctggcctccg
     1201 ggcggctgca acccgcagcc ctggggccag cggtgcctga actcattcca ggttggattg
     1261 acgagccgac cgtctcgccc gggcaggtgg caggcgtcgt cgtgaccatc gaccacttcg
     1321 gcaacctgct caccaacatc gatgtcgccc tggtgaggac gctgtctcac ccctgcatcc
     1381 gggtggccgg gcgggagctg ccgctgcggc gcacctactc cgacgtccgt ccgggcgaat
     1441 acctgggttt ggtcaactcc ttcggcgtcc tggagatcgc ccgggccgag atgagcgccg
     1501 ccgagggcct gggcctggac cgcggtgctc cggtcaccgt cgtacagaag tgaccgcaac
     1561 ctgctgatcg aaaaggactt gacggctcct ggccgcggaa cccgctgcgc cgcagcaggc
     1621 ggctcttgtc gcgccccgcg attcgtatat agttcgcagc cctgtgcccg gaatttcggg
     1681 cagcgcgttg aaatctcagg attttctcag ggccgtgaca tggaacgaga cgaggaacac
     1741 ttcgacctcg acgaggaatt cgtggccaac gcggaggaag aggactacga ccgcttcatc
     1801 gaccgcgccg accgacgcaa gcaggccgcg gtgaaacgtg gcaaggccgc ctggagcaag
     1861 ctcgaagacg tgctggccga gaagcgcctg cagaaggaac tgaaggacgt ctacgacgac
     1921 gtgtgagatt cattgatcgc ggccccggct cccttggcac cgggagccgc ccggggaccg
     1981 ctcgatgacc agcaataccg aatcccactg ggtcgtcctc aagtttggcg gcaccagcgt
     2041 ctcgagcgtc agcaactgga ggaacgtcgc cgccgtggtt cgcgcgcgac tcgacgaggg
     2101 cctgcgcccg atcgtcgtcc actcggcgct gtccgggatc accgatcgcc tcgagcagct
     2161 gctcgcgaag gcgctgactg gcgactggga gcccgtcatg gagcagatcg agcgccggca
     2221 tgtcgacctg tcgcgcgacc tcggcatccc gctcagcgcg gagctcgagc accacttcaa
     2281 cgacctgcgc cgcatggcct ccggcatcca cctgatcggc gaggtgagcg accggttgcg
     2341 cgcgcgcgtc atggcgaccg gcgagctgat ggcgacgcga atcggcgccg cttacctcgc
     2401 atcggagaag ctggacgtgc agtggctcga cgcacgcacg gtcctgcacg ccgaggaacg
     2461 ggccggcgca acggtgcggg cgaactacct ctccgccacc tgcagcttcg agccagacgc
     2521 ttcactccag cgtcgattgg cttccggcgg cagcgtgctg ctgacccagg gattcatcgc
     2581 gagcgacgcg aacggcgaga ccgtgctcct gggccgcggc ggctcagaca cgtcgggcag
     2641 ctatttcgcc gccaagctgc acgcgcgccg gctcgagatc tggacggacg tgcccggcat
     2701 gttcacggcg aatccgcgcg ccgtgccgaa cgcacggctg ctcaagtccc tcgactacga
     2761 cgaggcgcag gagatcgcga gcagcggggc caaggtgctc cacccgcgct gcatcctgcc
     2821 ggccaagcag tacggcatcc cgctgtcggt gcacgcgacg cagcaacccg acctcgaggg
     2881 aacggtcatc acgtcgacgg gcggcgatgg aggcgcacgc gtcaaggcgg tgtgcaccaa
     2941 gaagggcatc acgctcatct cgatggagac gctcgggatg tggcaccagg tcgggttcct
     3001 cgccgacgcc ttcgcggtgt tcaaggacca cggcctgtcc gtggacctcg tctcgacgtc
     3061 ggagacgacc gtcacggtgt cactggaccc cgcggcgaac gcgctcgaca cccgggcgct
     3121 cgagaccctc gtgaactcgc tgtcgaagct gtgccgtgtc gaggtcatcg gtccctgttc
     3181 cgccgtgagc ctcgtcgggc gcagcattcg tgccacgctc catcggctcg gcgacgcgct
     3241 ggagcttttc gaggagcagc ggatctacct ggtgacccag gccgcgaacg acctgaacat
     3301 caccttcgtc gtcgacgagg agcagggtga ccggctggtc agccgcctgc acgacgtgat
     3361 cgtgcgcaac gcgccgtcgg accacgtgct cggcccgacg tgggagcaga tccacggcat
     3421 cggaaccgcg caccctgttg gcgggccgcc gtggtggcag gggcgccgcg ccgaactcga
     3481 gaagatcgcg agggcgcagg gcagcgccta cgtctacgat cgagacacga tggtgcgcgc
     3541 ggcgaagcag ctcctcgcca tcgacggggt cgaacggatc ttctatgccg tgaaggcgaa
     3601 tccgcacccg gaggtgctgc gccagttcgc ggacctcggc ctcgcgttcg agtgcgtgtc
     3661 gcagggcgag tgcgagcggg tgatcgagac catcccgggc atcgcgcgcg accggatcct
     3721 gttcacgccg aacttcgcgc cgcgctccga atacgagtgg gcgatcgagg ccggcgtgca
     3781 cctcacgatc gacaacctgc acgccttgcg ccactggccg gaactgttcc gcggccgcga
     3841 cgtcctggtc cgcgtggaca cgggcttcgg gcgcggtcac cacgaccacg t
//
LOCUS       DAOMFD010000187         3867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_10818_length_3867_cov_25.466946, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000187 DAOMFD010000000
VERSION     DAOMFD010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10818_length_3867_cov_25.466946"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="PL152_09285"
     CDS             <1..574
                     /locus_tag="PL152_09285"
                     /EC_number="5.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HQR49520.1"
                     /translation="RPRRRPHAAPLLVPLIFVLAAGAGIFWLGTYASTTVVIVLRHAE
                     ATQDPQGDPELSPAGEERVSGLGEYVAGVLAGGKVDYLYAGDTRRAQETAAPIANQFG
                     LPINLLAPSDWDGFASRLKRDHRGKTIVVVGYATTIPGVVSRLAGTNVALSDSDYDSI
                     FVVVLPSPGSAKLFRLHYGPGTAGAKASAK"
     gene            complement(623..1081)
                     /locus_tag="PL152_09290"
     CDS             complement(623..1081)
                     /locus_tag="PL152_09290"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR49521.1"
                     /translation="MLKNGTRAPEFDLPDQDGRRHTLKQLLADGPLILYFYPADFTPG
                     CTAEACSFRDLHQDLMRAKLRVVGVSPQDVASHKRFAAKHDLNFPLLADPDKQAVKAY
                     DLDGPLGFGVRRGTYLIGTDGKIQDSVLADLRIGAHEAFVRKAVEAGARK"
     gene            complement(1084..1566)
                     /locus_tag="PL152_09295"
     CDS             complement(1084..1566)
                     /locus_tag="PL152_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR49522.1"
                     /translation="MNIHVRPAQPGDIDAIAARNIAMALEAEHKHLDPPTVRRGVQAV
                     FDTPAHGRYFIAEIDGRIVGQAMYTYEWSDWRNGVFWWFQSVYVTPDARRRGVFRALY
                     RHIEGLAKADPNVCGLRLYVERDNTRAQQTYARCGMQDAGYLVMEVDMSGAVRSARGG
                     "
     gene            1638..1713
                     /locus_tag="PL152_09300"
     tRNA            1638..1713
                     /locus_tag="PL152_09300"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1671..1673,aa:Ala,seq:cgc)
     gene            complement(1777..3060)
                     /locus_tag="PL152_09305"
     CDS             complement(1777..3060)
                     /locus_tag="PL152_09305"
                     /inference="COORDINATES: protein motif:HMM:NF023330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3422 domain-containing protein"
                     /protein_id="HQR49523.1"
                     /translation="MRLPPDHPLRVELNDEAHARPSEALRAPLRLTFLALSSGRGSGD
                     REWDHFCALLRRYEQQPPSSRVSHCSADCGPFRVKWERHSEFTRYKFIVAGPCDRPFD
                     SPALLEVPQDWLGELPGELVVATHAALLPAPPEPLDYDALAARHFHGNTLIGAGIAGG
                     AGLALTDFRIRDGFSRLLVLDRGMTPRQAGRMIQRLLEIDTYRLMALLALPVAQGLTP
                     WLNEAEAELAAISARLVESNEATEPLLLDRLTRLEAEIESRESAHHFRFTASEAYYAL
                     VERRITELREARIQGLQTFQEFIERRLAPAMNTCRSVSARVESLSQRVARATQLLSTR
                     VDVSRERQNQKVLESMNRRAEAQLRLQQTVEGLSTAAITYYVVGLVGYAAKGLKALGW
                     PLDTELVMALSIPFVALAALLGIRHLRKVVTRGAA"
     gene            3185..3260
                     /locus_tag="PL152_09310"
     tRNA            3185..3260
                     /locus_tag="PL152_09310"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3218..3220,aa:Arg,seq:cct)
     gene            3356..>3867
                     /locus_tag="PL152_09315"
     CDS             3356..>3867
                     /locus_tag="PL152_09315"
                     /inference="COORDINATES: protein motif:HMM:NF027404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49524.1"
                     /translation="MSDLKALLATTPLAAGNAPYVEEMYERFLLDPQSVDARWRDYFA
                     GMGGQGKDIAHGPLVEELARRAVAPRAGRPAKLAASAADVDRAAKQGAVSRLIQIYAN
                     RGHLVADIDPLGLMVRPVPKVLGLDYLGLTEADLDTEFLTGSRNDAIKPRLTLREIVA
                     QLKHIYCGTIG"
BASE COUNT          613 a         1314 c         1305 g          635 t
ORIGIN      
        1 ccgtccgcga cgacgaccgc acgcggcacc gctgctcgtg ccgctgatct tcgtgctcgc
       61 ggcaggcgcc ggaatcttct ggctcgggac atacgcgagc accaccgtgg tcatcgtgct
      121 gcgccatgcc gaggcgacgc aggatcctca aggcgatccg gaactcagtc cggcgggcga
      181 ggagcgcgtc agcggactcg gtgagtatgt cgccggtgtg ctggccggcg ggaaggtgga
      241 ctacctgtac gccggggaca cccgtcgtgc ccaggagacg gcggcgccca ttgccaatca
      301 gttcgggttg ccgatcaacc tgcttgcgcc gagcgactgg gacggcttcg cgagccggct
      361 caagcgcgat caccggggca agacgatcgt cgtcgtcggg tacgcgacca cgattcccgg
      421 cgtcgtgagc cggctggccg gcacgaacgt cgccctgagc gacagcgact acgactcgat
      481 cttcgtcgtc gtactgccga gccccggttc ggccaagctc ttcaggctgc actacgggcc
      541 cggcacagcc ggggccaagg cgtcggccaa gtagtcagcc gtttccgagg aatgccgcct
      601 aacccgctaa tttataaaca ggttattttc gagcgccggc ctcgacggct ttgcgcacga
      661 acgcttcgtg cgccccgatc cgcaggtcgg cgagcaccga gtcctggatc ttgccgtcgg
      721 tgccgatcag gtaggtcccg cgtcgcacgc cgaaacctag cgggccgtcg aggtcgtagg
      781 ccttcaccgc ctgcttgtcg gggtccgcga gcagcggaaa gttcaggtcg tgcttggcgg
      841 cgaagcgctt gtgtgacgcc acgtcctgcg gactcacgcc gaccacgcgc agcttcgcgc
      901 gcatcaggtc ctggtgcagg tcgcggaagc tgcaggcctc cgcggtgcag cccggcgtaa
      961 agtcggccgg gtagaagtag aggatcagcg gcccgtccgc gagcagctgc ttcagcgtgt
     1021 gcctgcgccc gtcctggtca ggaaggtcga actcgggtgc cctggtgccg ttcttcagca
     1081 tgttcatcct cccctggcgc tgcgtaccgc gccggacatg tccacttcca tcacgaggta
     1141 tcccgcgtcc tgcatgccgc aacgcgcgta ggtctgctgc gcgcgcgtgt tgtcgcgctc
     1201 cacgtacagc cggaggccac agacgtttgg gtcagccttc gccaggccct cgatgtgccg
     1261 gtacagggcg cgaaacacgc cgcgacgccg cgcgtcgggt gtcacataga cactttggaa
     1321 ccaccagaac acgccgttgc gccagtcgct ccactcgtat gtgtacatgg cctggccgac
     1381 gatccggccg tcgatctcgg cgatgaagta gcggccgtga gcgggcgtgt cgaatacggc
     1441 ctgcactcca cggcgcaccg tcggcgggtc gaggtgcttg tgctcggcct cgagcgccat
     1501 cgcgatgttg cgggccgcga tggcgtcgat atcgccgggc tgcgcagggc gaacgtggat
     1561 gttcatggcg cgatcataca ggcgacccac cgtggttgcg ggtgcagggg ctggtgctac
     1621 aatgcgcgcc ctttcgtggg gcggtagctc agctgggaga gcgtcgcgtt cgcaatgcga
     1681 aggtcgaggg ttcgatcccc ttccgctcca ccacgacacg acggccgggt cactcgaccc
     1741 ggccgttttt ccattcgggc cgcgctgacg gccccctcag gccgcgccgc gcgtcacgac
     1801 ttttcgcagg tgtcggatgc cgagcagggc cgccagcgca acaaacggga tgctgagcgc
     1861 catgaccagc tccgtatcca acggccagcc gagcgccttg aggcccttgg ccgcataccc
     1921 caccaggccc accacgtagt aggtgattgc ggccgtcgac aggccctcga cggtctgctg
     1981 gagcctgagt tgagcctcgg cccgacggtt catcgattcg agcaccttct ggttctggcg
     2041 ctcgcgcgac acgtcaacgc gcgtcgagag caactgagtg gcccgtgcga cgcgctggga
     2101 caatgattcg acgcgcgcag acaccgatcg gcacgtgttc atggctggag ccagccggcg
     2161 ctcgatgaat tcctggaagg tctgcagccc ctggatgcgg gcctcgcgca gttcggtgat
     2221 ccgacgttca acgagcgcgt agtaagcctc ggacgccgtg aagcggaagt ggtgcgcgga
     2281 ctcgcgactc tcgatctcgg cctcgaggcg cgtgagccgg tcgagcagca gcggctcggt
     2341 ggcctcgttc gactcgacca gccgggcgct gatggccgcc aattcggcct ccgcctcgtt
     2401 gagccagggc gtcaggccct gggcgaccgg cagcgcgagc agcgccatca gccggtaggt
     2461 gtcgatttcg agcagacgct ggatcatgcg accggcctgg cgcggtgtca tgccgcggtc
     2521 cagcacgagc agccgactga agccgtcgcg gatgcggaag tccgtcaacg cgagtccggc
     2581 tccgccggcg atgcccgcgc cgatcagagt atttccgtgg aaatgccgag cagcgagcgc
     2641 atcgtagtcg agcggctcgg gtggtgcggg cagcagcgcg gcatgggttg ccaccaccag
     2701 ttcgcccgga agctctccga gccagtcctg cggcacctcc aggagtgccg gcgagtcgaa
     2761 cggtcggtcg cacggtcccg ccacgatgaa cttgtagcgc gtgaactccg aatgtcgttc
     2821 ccacttcacc cggaatgggc cgcagtccgc gctgcagtgg ctcaccctgg acgacggggg
     2881 ctgctgttcg tagcgccgca gcaacgcgca gaagtggtcc cattcgcggt cccccgagcc
     2941 gcgtccggac gacagcgcca ggaaggtgag ccgcaacggg gctcgcaggg cctcggacgg
     3001 ccgcgcgtga gcctcgtcgt tcagttcgac gcggaggggg tggtcggggg gcagtcgcac
     3061 aggtcgggcc tcctcaccca gtctagacgt cggcccgtcc tgcccgcctc gcggcaagcc
     3121 acaatgccgg cgggcgcttc gctgtaccgc tcgccgtcgc cgtgccgtta cacttcgccc
     3181 gcgcgccccg gtagctcagt ggatagagca accgcctcct aagcggtagg tcgtcggttc
     3241 aactccggcc cggggcgcca tccgccccaa ggaatcgacg gacttcgcta gaatgtccgg
     3301 cctttcgcca ggccagcacc acgcgccggg tgacgccggg tatcgatcca aaaaaatgag
     3361 cgatctcaag gcattgcttg cgaccacccc gctcgccgcg ggcaacgcgc cgtacgtcga
     3421 ggagatgtac gagcgtttcc tcctcgaccc gcagtcggtc gacgcgcgct ggcgggacta
     3481 cttcgccggc atgggagggc aggggaagga catcgcgcac ggcccgctgg tcgaggaact
     3541 cgcgcgtcgc gccgtcgcgc cgcgtgcagg ccgccccgcg aagttggcag cgagtgcggc
     3601 agatgtcgac cgcgcggcga agcagggcgc cgtctcacgc ctcatccaga tctacgcgaa
     3661 tcgcggccac ctcgtcgccg acatcgaccc gctgggtctc atggtccgtc cggtgcccaa
     3721 ggtgcttgga ctcgactacc tgggtctgac cgaggccgac ctcgacaccg agttcctcac
     3781 cggcagccgc aacgacgcca tcaagccccg actcacgctg cgcgagatcg tcgcgcagtt
     3841 gaagcacatc tactgcggca cgattgg
//
LOCUS       DAOMFD010000188         3821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11070_length_3821_cov_24.783324, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000188 DAOMFD010000000
VERSION     DAOMFD010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3821
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11070_length_3821_cov_24.783324"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="PL152_09320"
     CDS             <1..742
                     /locus_tag="PL152_09320"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQR49525.1"
                     /translation="SFELTTHNPGGHSSQPRADNAIYELADALETLKDYRFPVMWNDW
                     TLGSFRASAKVTPGPLGAAMAKFAANPRDGSAADVLSANPLIVGKLRTTCVATMLRGG
                     HAENALPQSATATVNCRVFPGVPVSDVQSTLQRLAGAKVEVKLIGHPHPSDPSPVRPD
                     VVDAVTRAVHADYPGVPIVPEMAAYATDGSIFRAAGIPTYGVSSLFIKGSDEFSHGLN
                     ERISTDAFYAGLEHWYVLATTFGGKPAP"
     gene            complement(756..1472)
                     /locus_tag="PL152_09325"
     CDS             complement(756..1472)
                     /locus_tag="PL152_09325"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HQR49526.1"
                     /translation="MKLDPAHIVAARPYPQWRFLQLTLAIIAWMLVAPLLETRWTGHL
                     AMQVLLLDLMLVTMWANPHWARVRQVVLVLWLLSLATSVLSVTGLSQMLSKFDETLDV
                     SFAIPVACACIVGVLAFAFRAERPTVDGIFAMVVAYLLIALVFAELHYLLLIWNPEAL
                     HLTVPLPQQSVRELRGDLIYFSLVTLSTVGYGDILPVSPTARVLAMLEALIGQFYVAV
                     VVATFVAKYVALRDRQARKD"
     gene            complement(1605..2072)
                     /locus_tag="PL152_09330"
     CDS             complement(1605..2072)
                     /locus_tag="PL152_09330"
                     /inference="COORDINATES: protein motif:HMM:NF016060.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HQR49527.1"
                     /translation="MADDAIDRSAARARHWRTRFLRLPEKARLAIVAVVGALIGLATY
                     EIIYFLNPFEPRAPTSWLTAFIIAVPRQYSLHRSLTFASSVPYVPRLGRAYVLYGCVA
                     LVTTSLDWLLVERLAVPHQFAWLACISTTGIINLFALKPLVFGDARRGGRSGA"
     gene            complement(2079..2813)
                     /locus_tag="PL152_09335"
     CDS             complement(2079..2813)
                     /locus_tag="PL152_09335"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HQR49528.1"
                     /translation="MIADIVAVTLPGFAIGAIFMAIANRRVTLEVARARWLKIGVYFL
                     IVHAVLGVAAAGRPWVTLLVAAILAAGCVELRGAWHRIPEPRPGRIWLVYAIVAIAAA
                     GASWLLAPEAFAFLFLVTATFDGFSQVVGQLVGRRRIAPTVSPAKTVGGVAGGLFAAA
                     AVALLVRDLLAGALKPSLFATAVATAAICAAGLAGDLAASWVKRRAGLKDYSAALPGQ
                     GGFLDRFDSLLGALAVVGAALVAYGG"
     gene            complement(2810..3688)
                     /locus_tag="PL152_09340"
     CDS             complement(2810..3688)
                     /locus_tag="PL152_09340"
                     /inference="COORDINATES: protein motif:HMM:NF012981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HQR49529.1"
                     /translation="MRWLAIANPAAGRTHEAERMVSRLGTIPAVHVEVARTCAPGDAS
                     RLAREAEGFDGVIAIGGDGTIAEVLQGMDLARQRLAVLPAGHGNCLARDLGVCSADRA
                     LAALTSGSSRRLDLMDVHVRFATGHEDQRLCASTLAVGYVTDVVMFGRQRLHALGRAA
                     YAAAAVVVVPRSFEASVAGSQERDRPQRYTGLVVNNTMHLANFRGFPDASVDDGMLDV
                     MEQRYGWPRQLLHNGAVLARSRAFGPARLRQAAAEEFELHAARTLMADGELLQNVVHV
                     AVRCRPRAVTCVAGPS"
BASE COUNT          677 a         1297 c         1328 g          519 t
ORIGIN      
        1 cagtttcgag ctgacgacgc acaacccggg tggccacagc tcgcagccgc gtgccgataa
       61 cgcgatctac gagctggccg atgcgctcga gacgctcaag gactaccgtt tcccagtcat
      121 gtggaacgac tggacgctcg gcagcttccg cgcctctgcg aaagttacgc ccggccccct
      181 cggagcggcg atggcgaaat tcgctgccaa cccgcgtgac gggtcggcgg ccgacgtgct
      241 ctcggccaac ccgctgatcg tgggcaagct gcgcaccacc tgcgtcgcca cgatgctcag
      301 gggtggacat gcggagaacg cgctgccgca gtccgcgacc gcgaccgtca attgccgcgt
      361 atttccgggc gtccccgtgt ccgacgtgca atcgacgttg cagcggctgg ccggcgcaaa
      421 ggtggaagtg aagctcatcg gccacccgca tccgagcgat ccgtcaccgg tgcgcccgga
      481 cgtggtcgac gccgtgacca gggccgtgca cgcggactat cccggcgtgc cgatcgtgcc
      541 ggagatggcc gcgtacgcca cggacggctc gatcttccgc gccgccggta tcccgacgta
      601 tggcgtgagc agcctgttca tcaagggcag cgacgagttc tcgcacggcc tgaacgagcg
      661 catctcgacg gacgcgttct acgcggggct cgagcactgg tacgtgctcg cgacgacgtt
      721 cgggggcaag cccgcgccct gagctccgct ggcgttcagt ccttgcgcgc ctgccggtcg
      781 cgcagcgcca cgtacttcgc gacgaaggtc gccacgacga cggcgacgta gaactggccg
      841 atgagcgcct ccagcatcgc aagcacgcgt gccgtcgggg acaccgggag gatgtcgccg
      901 tagcccacgg tggacagcgt caccaggctg aaatagatga ggtcgccgcg cagctcgcgc
      961 acgctctgtt gcggcagagg cacggtcagg tgcaacgcct cgggattcca gatcagcaac
     1021 aggtagtgga gctcggcgaa taccagggcg atcagcaggt aagccacgac catcgcgaag
     1081 atcccgtcga cggtcgggcg ctcggcgcgg aatgcgaacg cgagaacgcc gacgatgcag
     1141 gcgcaggcca ccggtatggc gaaggacacg tcgagcgtct cgtcgaactt cgacagcatc
     1201 tgggaaaggc ccgtgacgga caggacggac gtggccagcg acagcagcca gaggaccagg
     1261 actacctgac ggacccgcgc ccagtgcggg ttcgcccaca tcgtgaccag catcaggtcc
     1321 agcagcagga cctgcatggc caggtgtccc gtccatcgcg tctccagcag gggcgcgacc
     1381 agcatccagg cgatgatcgc gagcgtgagc tgcaggaacc gccattgcgg gtacggccgt
     1441 gcggcgacga tgtgtgctgg gtcgagcttc atggcgtagc ctggcgtggg cgaaaagcgc
     1501 gttcggcgac gacggtattc ccgggcgtgt cgctccgcaa gggtcggtgc gacggacatt
     1561 ccgggggagg cgcggactgc acctcgggca agcgagtctc gccgctatgc acccgatcgt
     1621 ccaccgcgcc gtgcatcacc gaagaccagc ggcttcagcg cgaagagatt gatgatcccg
     1681 gtcgtcgaga tgcaggcaag ccacgcaaac tggtgcggca ccgccagccg ctccaccagg
     1741 agccagtcga gcgatgtcgt gaccagcgcg acgcacccgt acaggacata ggcacggccg
     1801 aggcgcggca catacggaac ggaggaggcg aacgtgagcg agcggtgcag cgagtactgt
     1861 cgcggcaccg caatgatgaa ggcggtgagc cagctggtcg gcgccctggg ctcgaatgga
     1921 ttcaggaagt agatgatctc gtaggtggcg agcccgatga gcgcgccgac gaccgcgacg
     1981 atcgccagtc gggccttctc cggcagccgc aggaatctcg tccgccagtg gcgggcgcgc
     2041 gctgcgctgc gatcgatggc gtcgtctgcc acggctagtc agcccccgta ggccaccagc
     2101 gcagcgccca cgacggcaag cgcgccgagc aggctgtcga agcgatccag gaatcctccc
     2161 tgtcccggca gcgccgcgga atagtccttg agacctgcgc gtcgcttgac ccaggaagcc
     2221 gcgaggtcgc cggccaggcc cgccgcgcaa attgcggcgg tcgcgactgc ggtcgcgaaa
     2281 agggaaggtt tcaatgcgcc ggccaacaga tcgcgcacca gcagcgcgac cgcagccgcg
     2341 gcgaagagtc cgcccgcgac cccgccaacc gtcttggcag ggctgaccgt gggtgcgatg
     2401 cgtcgccgac cgaccaactg gcccacgacc tggctgaagc cgtcgaatgt cgccgtgacg
     2461 aggaacagga atgcgaacgc ctccggtgcg agcagccagc tcgcgccggc cgccgcgatt
     2521 gcgacgattg catacacgag ccagatgcgc ccgggccggg gctcgggaat tcggtgccag
     2581 gcaccgcgca gttcgacgca gcctgcggcg aggatcgccg cgacgagcag cgtcacccat
     2641 gggcggcctg cagcggcgac gcccagcacg gcgtgcacga tcaggaagta gacgccgatc
     2701 ttgagccagc gtgcacgagc cacctccaag gtcacccggc gattcgcgat cgccatgaag
     2761 atcgcgccga tggcgaagcc cggcaatgtg acggcgacga tgtcggcgat cacgatgggc
     2821 ctgcgacgca ggtcaccgcg cgtgggcggc agcggacggc gacgtgaacg acgttctgca
     2881 gcaattcacc gtctgccatc agggtccgcg cggcgtgcag ctcgaattcc tcagccgccg
     2941 cctggcgcag acgggcaggg ccgaacgcac gactgcgtgc gaggacagcg ccgttgtgca
     3001 gcagctgtcg cggccagccg tagcgctgct ccatcacgtc gagcatgccg tcgtcgacgc
     3061 tggcgtccgg gaaaccgcgg aagttcgcga ggtgcatcgt gttgttcacc accagcccgg
     3121 tgtagcgctg tggccgatcg cgctcctgcg atccggcgac gcttgcctcg aacgagcggg
     3181 ggacgacgac aacggccgcc gcggcatagg cggcccgacc cagtgcgtgc agtcgctggc
     3241 gtccgaacat gacgacatcg gtgacgtaac cgacggcaag cgtactcgcg cagagccgtt
     3301 ggtcctcgtg gccggtggcg aaccggacgt ggacatccat caggtccaaa cggcgggaag
     3361 atccactggt cagggccgcc agtgcgcggt cggcgctgca gacgccgagg tcgcgcgcga
     3421 ggcagttgcc gtgcccggcg ggcagtaccg cgaggcgctg ccgggcgaga tccatcccct
     3481 gcagcacctc ggcgatcgtc ccgtcgccgc cgatcgcaat cacgccgtcg aatccttcgg
     3541 cctcgcgcgc cagtcggctc gcgtcacccg gtgcgcaggt gcgggcgacc tcgacgtgca
     3601 cggcggggat cgtgcccagc cggctcacca tgcgctccgc ctcgtgggtg cggccggccg
     3661 cgggattggc aatggccagc cacctcacgc agccgccccg gcgcgacgtt cccgcagcac
     3721 ccagtagagg ccgcgcacgt cggaccgcca ttccggcagc accgcgagca gcagcagttc
     3781 ctcgaggttg ccgagcacgc tcgcggcgac ggccgcgtag a
//
LOCUS       DAOMFD010000189         3723 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11587_length_3723_cov_36.669575, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000189 DAOMFD010000000
VERSION     DAOMFD010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11587_length_3723_cov_36.669575"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..400)
                     /locus_tag="PL152_09345"
     CDS             complement(<1..400)
                     /locus_tag="PL152_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HQR49530.1"
                     /translation="MRAMVLAAGRGERMRPLTLERPKPLLEVGGLPLIVHHLHALAMA
                     GFVDVVVNVSWLGDQIRAALGDGSRYNVRLRYSDEGPEPLETGGGVFRALPLLGPAPF
                     LVLNGDIWTDYPYAKLRESLRRDDLAHLVLV"
     gene            complement(447..1481)
                     /locus_tag="PL152_09350"
     CDS             complement(447..1481)
                     /locus_tag="PL152_09350"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HQR49531.1"
                     /translation="MTEPRRDDPRLALLRHWLEHGLGWKDAELAPASADASFRRYFRI
                     TRPGGTCIAMDAPPDKENVEPYLAVAAMLADIGVHAPRVLDRNAADGFLLLTDLGSVT
                     YLMELEDQTRSDALYLDAVAALVRMQARGGVHAGRLPPYDEKLLRFEMSLFTDWLLGR
                     HLGIALDAAESRHLAAAFDRLVANALEQPRVFVHRDYHSRNLMVCAGANPGILDFQDA
                     VHGPLTYDLVSLLRDCYIAWPQDRVVAWALEYRRQATAAGLACGTDQQQFLRWFDLMG
                     VQRHLKASGIFARLWHRDGKPGYLPDVPRTLEYVGQACARHPDLAPLGQLVRERVLPA
                     MQASLEARRA"
     gene            1535..>3723
                     /gene="lptD"
                     /locus_tag="PL152_09355"
     CDS             1535..>3723
                     /gene="lptD"
                     /locus_tag="PL152_09355"
                     /inference="COORDINATES: protein motif:HMM:NF016348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0010033 - response to organic substance
                     [Evidence IEA];
                     GO_process: GO:0061024 - membrane organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HQR49532.1"
                     /translation="MKPSARTTTYRRRRLATVALALMPLPAYTAGTVEQACRIPVTED
                     PRLEMLRKADPDDPHIDVTSDHGDLSQQGDAELSGNVSIRMGQRLLTSDEATIDSTNR
                     SITVKGKVEYLDPTIHVTGREGEFEKGAGDFEGAKFDLVDGSGRGSAKSARLHDQTML
                     DLDTVRYTVCPPGNDDWILKAQKISLDQEAKTGTARDVRLDFLGVPILYTPWISFPLS
                     DERKSGLLFPAIGSGGQSGTQVVIPWYWNIAPNYDATFTNRWYSSRGYRIDPEFRFLT
                     ERSHGVFNAQVMPHDRELDEYRGLYDLKETTLFDSKTRALIDAAYVTDRSYFEDFGAG
                     FEGTSVIFLNQLGELRRDTEHWTLVGRAQNYQVLDPALAYDEYPYAMLPQLVVGGRWD
                     GLGRGFSAALDAEAVNFYRATGPQGFRLDAEPALTWGIGGRGGYVDATAAWRYTTYAL
                     RDTAPGTDNSLERSLPIISVDSGFVLERPVGSKDQRLQTLEPRIQYVYVPYRNQDDIP
                     IFDTDQPTLNMVELFRTNRYVGADRVGDANLVSTGLTTRLLDASSGQQYLSATIGEAF
                     YFQQPRVTLPGETPTNRSSSNVIAEIDLEAFKHWSSRLGYEWDPDANQTERMDFGVQY
                     RPASDRVLNAVYRYQRDQLEQFDVSAAWPVLENWHGFARWVYSLSENKTLDQFVGVEY
                     SSCCWAIRLITRHFVSNRSGNSDNSIGFQLELKGLSSVGVDNEAFLR"
BASE COUNT          650 a         1290 c         1237 g          546 t
ORIGIN      
        1 cgaccagcac gaggtgcgcc aggtcgtccc gtcgcagcga ctcgcgcagc ttcgcgtagg
       61 gatagtcggt ccagatgtcg ccgttcagca ccaggaacgg cgccgggccc agcagcggca
      121 gcgcgcggaa gacgccaccg cccgtctcga gcggctcggg accttcgtcg ctgtaccgga
      181 gcctcacgtt gtaccgcgag ccgtcgccga gggccgcccg gatctggtcg ccgagccatg
      241 agacattgac gaccacgtcg acgaatcccg ccatcgcgag cgcgtgcagg tgatgcacga
      301 tgagcggcag tccgccgacc tcgagcagcg gcttgggccg ctccagcgtg agcgggcgca
      361 tgcgttcgcc gcgtccagct gccaatacca tggctctcat ggacgccgcg cctcgagccc
      421 tgccttcacc gccagcacca tggctctcat gcgcgccgcg cctcgaggct ggcctgcatc
      481 gcgggcagga cgcgctcccg caccagctgc ccgagcggcg caaggtccgg atgccgcgcg
      541 caggcctgcc cgacgtactc gagcgtgcgc ggtacgtccg gcaggtagcc gggcttgccg
      601 tcgcggtgcc acaggcgcgc gaagatgccg ctcgccttca ggtgccgctg cacccccatc
      661 aggtcgaacc agcgtaggaa ctgctgttgg tccgtgccgc aagcaagccc ggcggccgtc
      721 gcctggcgcc tgtactcgag cgcccacgcg acgacgcggt cctgcggcca cgcgatgtag
      781 caatcccgga gcagcgacac caggtcgtag gtgagcgggc cgtggacggc atcctggaag
      841 tcgaggattc cgggattcgc accggcgcag accatgaggt tgcgcgaatg gtagtcgcgg
      901 tgcacgaaca cccgtggctg ctcgagcgcg tttgcgacca gccggtcgaa cgcggccgcg
      961 aggtgccgcg actcagcggc atcgagtgca atgccgaggt gccgcccgag cagccagtcg
     1021 gtgaacaggc tcatctcgaa ccggagcagc ttctcgtcgt acggcggcag ccggccggca
     1081 tggacgccgc cgcgcgcctg catgcgcacg agcgcggcga ccgcgtccag atagagcgca
     1141 tcggagcgcg tctggtcctc gagttccatc aggtaggtga cggatcccag gtccgtgagc
     1201 agcaggaagc cgtcggccgc gttccggtcc aggacgcgcg gcgcatggac gccgatgtcg
     1261 gccagcatcg ccgcgacggc gaggtacggc tcgacgttct ccttgtccgg aggggcatcc
     1321 atcgcgatgc atgtcccgcc cggccgcgta atccggaaat accggcggaa gctggcatcc
     1381 gccgaggcgg gcgccagctc tgcatccttc catccgagcc cgtgctcgag ccaatggcgc
     1441 agcagcgcga gccgcgggtc gtctcggcgg ggttcggtca aggcaggcct cggaaagtcg
     1501 ggtgcattcg ggggcggcaa ttataatcgc aacgatgaag ccgtctgcgc gcacaacgac
     1561 ctaccggcga cggcggctcg cgacagtggc actggcgctg atgccactgc ccgcatacac
     1621 cgccggcacc gtcgaacagg cctgccggat accggtgacc gaggacccac ggctcgagat
     1681 gctgcgcaag gcggaccccg acgatccgca catcgacgtc accagcgacc acggcgacct
     1741 ctcgcagcag ggcgacgcgg aactctcggg aaacgtcagc atccgcatgg gacagcgcct
     1801 gctcacgtcg gacgaagcca cgatcgactc cacgaatcgc agcatcacgg tcaagggcaa
     1861 ggtcgagtac ctcgacccga ccatccacgt gacgggccgc gaaggcgagt tcgagaaagg
     1921 cgccggcgac ttcgagggcg ccaagttcga cctggtggac gggtccggac gcggatccgc
     1981 gaagtcggcg cggctgcacg accagacgat gctggacctc gacaccgtcc gctacaccgt
     2041 gtgcccgccg ggcaacgacg actggatcct caaggcgcag aaaatctcgc tcgaccagga
     2101 agcgaagact ggcacggcgc gggacgtccg gctggatttc ctgggtgtcc cgatcctcta
     2161 cacgccgtgg atctccttcc ccttgagcga cgagcgcaag tcgggcctgt tgttcccggc
     2221 gatcggcagc ggcggccagt ccggcacgca ggtcgtgatc ccgtggtact ggaacatcgc
     2281 gccgaactac gacgccacgt tcaccaatcg ctggtactcc tcgcgcggct accgcatcga
     2341 cccggagttc cgcttcctca ccgagcgcag ccacggcgtg ttcaacgcac aggtcatgcc
     2401 gcacgaccgg gaactcgacg aataccgggg actctacgac ctcaaggaaa ccacgctgtt
     2461 cgattcgaag acgcgcgccc tgatcgacgc ggcttacgtc accgatcgct cctacttcga
     2521 ggacttcggc gcgggcttcg agggtaccag cgtcatcttc ctgaaccagc tcggcgaact
     2581 gcggcgggac accgagcact ggacactcgt cggccgcgcc cagaactacc aggtgctcga
     2641 tccggcgctt gcgtacgacg agtacccgta cgcgatgctg ccgcagctcg tggtcggcgg
     2701 ccgctgggac gggctcggtc gcggattcag cgcggcgctc gacgccgagg cggtcaactt
     2761 ctaccgcgcg acgggcccgc agggattccg gctggatgcc gagcccgcgc tcacctgggg
     2821 catcggcggc cgcggcggct acgtcgacgc aacggcggcc tggcgctaca cgacttacgc
     2881 cctccgcgac accgcgccgg gcaccgacaa cagcctggaa cgttcgctcc cgatcatcag
     2941 cgtcgattcc gggttcgtgc tcgagcgccc cgtcgggtcg aaggaccagc gcctgcagac
     3001 cctggaaccg cggatccagt acgtgtacgt gccgtaccgg aaccaggacg acatcccgat
     3061 cttcgacacg gaccagccga cgctcaacat ggtcgagctg ttccgcacca accgctacgt
     3121 cggcgccgat cgcgtcggcg acgcgaacct ggtcagcacg ggactcacca cccggctgct
     3181 ggatgcgtcg agcggccagc agtacctgag cgcgacgatc ggcgaggcct tctatttcca
     3241 gcaaccgcgc gtgaccctgc cgggggagac gccgaccaat cgctcgagct ccaacgtgat
     3301 cgcggagatc gacctcgagg cgttcaagca ctggagcagc cggctgggct acgagtggga
     3361 tcccgacgcg aaccagacgg agcgcatgga cttcggcgtg cagtaccgcc cggcgagcga
     3421 ccgcgtgctg aacgcggtct atcgctacca gcgcgaccag ctcgagcagt tcgacgtctc
     3481 ggccgcgtgg ccagtcctcg agaactggca tggtttcgcc cggtgggtgt attcgctgtc
     3541 ggaaaacaag accctcgacc agttcgtggg cgtcgaatac tcgtcgtgct gctgggcgat
     3601 ccgcctgatc acgcggcact tcgtgagcaa tcgctcgggg aactcggaca actcgatcgg
     3661 cttccagctg gaactgaagg gcctgtcgag tgtcggagtg gacaacgaag ctttcctgcg
     3721 gga
//
LOCUS       DAOMFD010000190         3703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11693_length_3703_cov_34.549342, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000190 DAOMFD010000000
VERSION     DAOMFD010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3703
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11693_length_3703_cov_34.549342"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..456
                     /locus_tag="PL152_09360"
     CDS             <1..456
                     /locus_tag="PL152_09360"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186037.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HQR49533.1"
                     /translation="AASSSSISDGAAAVVVASGAFAASRGLRPLARVAAYASHAQAPE
                     WFTTAPAPAIHKALERAGWKVSDVDLFEINEAFACVTMAAMRDLGIDHSRVNVNGGAC
                     ALGHPIGATGGRLITTLVHALRKRGLRRGIASQCIGGGEATAIAIEITG"
     gene            496..1257
                     /locus_tag="PL152_09365"
     CDS             496..1257
                     /locus_tag="PL152_09365"
                     /inference="COORDINATES: protein motif:HMM:NF006072.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR49534.1"
                     /translation="MQLKDKVVVVTGGARGIGRAIASAFAEQGARVAIVDLNQTDLEK
                     AAAEFAASGVNVRTYTANVARENEVIAALDAVVRDHGRLDVMVNNAGIIKDGLLVKAK
                     DGAVTGKLGLEQWQAVIDVNLTGVFLCGREAAERMVRLGHGGVIINISSISKCGNAGQ
                     TNYSAAKAGVAAMAVVWAKELARYGIRAASIAPGFTRTDLLAGMPPEMLEKVTAPVPL
                     KRLALPEEIGHTAVYIAENDYFTGRSVEIDGGLRL"
     gene            1286..2053
                     /locus_tag="PL152_09370"
     CDS             1286..2053
                     /locus_tag="PL152_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HQR49535.1"
                     /translation="MQLQDVRAIVTGGASGLGLAVVESVIAAGGRATILDVNEPAGAQ
                     AVARLGDRAAFIKTDVTSEGTVDQAVQVATASMGGLSLAVSCAGVGWPKRMVGKEGPM
                     PGEFFRKVIEINLVGTLLVSKAAAAAMQKNAPNAGGERGAIVMTASVAAFDGQIGQVA
                     YSASKGGVVGMTLPMARELAAFGIRVVTIAPGLFLTPMMAALPQEAQDSLGKQVPFPP
                     RLGNPAEFASLVRQIVENPMLNGETIRLDGAIRMAPR"
     gene            2145..>3703
                     /locus_tag="PL152_09375"
     CDS             2145..>3703
                     /locus_tag="PL152_09375"
                     /inference="COORDINATES: protein motif:HMM:NF017038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron-transfer flavoprotein:ubiquinone
                     oxidoreductase"
                     /protein_id="HQR49536.1"
                     /translation="MSDMNQAAVERDQMEYDVVVVGAGPAGLACAIRLKQLKPDLNVC
                     VLEKGASIGAHMLSGAVLEPGPLDQLLPGWRDEHKGLRVPAGRDEFSFLTKTKRFRLP
                     TPPQMNNHGNFIVSEGGLVEYLGTKAEGLGCDVFAGFAAASPLFDDQGAVVGARIGDM
                     GLEKDGEPGPNFAPGPEIRARTTILAEGCRGSISKILIKRYGLDQGKSPQTYGLGFKE
                     LWQLPKGRVEAGLIQHTVGWPMDAATYGGSFMYHLDQDRLYVGFVVGLDYEDPRFQPF
                     EAYQQFKNHPSLRPLFEGGEVLAYGARTIIEGGWQSMPTLEMPGALLIGDAGGTLNVT
                     KIKGIHQAIRSAVLAAEHIVEHGHTAGYDQRWRASEGGRELHKVRNIRPGFRHGMWVG
                     LANGALETLTAGHSPWTLQNHADHSALKRLDQYTSPDRGWVERSLPPRDRLAAVYFAG
                     NTHEEAQPIHLKVADTSVCATRCVTEYGNPCQNFCPAHVYEMVDDGAGGKKLQINASN
                     CVHCKACDIKDP"
BASE COUNT          637 a         1249 c         1281 g          536 t
ORIGIN      
        1 gcggcatcct cgtcgtccat ctccgacgga gccgcggccg tcgtcgtggc gagtggcgca
       61 tttgccgctt cccgcgggct caggccgctc gcgcgcgtcg cggcctacgc gagccacgct
      121 caggcacccg aatggttcac gaccgccccg gcccctgcga tccacaaggc gctcgaacgc
      181 gccgggtgga aggtgtcgga cgtcgatctg ttcgagatca acgaggcctt cgcgtgcgtg
      241 acgatggctg cgatgcgcga cctcgggatc gaccattccc gcgtcaacgt gaatggcggc
      301 gcatgcgcgc tcggccaccc gatcggcgcc accggcggcc ggctgatcac cacgctcgtg
      361 cacgcgctca ggaagcgcgg cctgcgtcgc gggatcgcct cacagtgcat cggcggcggc
      421 gaggccacgg ccatcgccat cgagatcacc ggctgaacca gcgcgcgtgt cgtcagttaa
      481 cgcaagggtc gaagcatgca gttgaaggac aaggtcgtcg tcgtcaccgg cggcgcgcgc
      541 ggcatcggca gggccatcgc atcggcattc gccgagcagg gcgcgcgggt cgcgatcgtg
      601 gacctgaacc agaccgatct cgagaaggcg gctgccgaat tcgcggccag cggcgtgaac
      661 gtccgcacct acaccgccaa cgtcgcgcgc gagaacgagg tgattgcggc gctcgatgcg
      721 gtggtccgtg accacggcag gctcgacgtg atggtgaaca acgccggcat catcaaggac
      781 ggcctgctcg tcaaggcgaa ggacggcgcg gtaaccggca agctgggcct cgagcagtgg
      841 caggcggtca tcgacgtgaa cctgaccggc gtgttcctgt gcggccgcga ggccgcggag
      901 cgcatggtcc ggctcggcca cggcggcgtc atcatcaaca tctcgagcat ctcgaagtgc
      961 ggcaacgccg ggcagacgaa ctactcggcc gcaaaggccg gcgtcgccgc gatggccgtg
     1021 gtctgggcga aggaactggc ccgctacggg attcgcgccg cctcgatcgc gccggggttc
     1081 acgcgcaccg acctgctggc cggcatgccc cccgagatgc tggagaaggt caccgctccg
     1141 gtaccgctga agcgcctcgc cctgcccgag gagatcgggc acacggcggt gtacattgcc
     1201 gagaacgact acttcaccgg gcggtccgtg gagatcgacg gcggactgcg gctctgaccc
     1261 cgtaagaacg cggagagacc aggttatgca gttacaggac gttcgagcca tcgtcaccgg
     1321 cggcgcctcg gggctcggcc tcgcggtcgt cgagagcgtc atcgccgctg gcggccgggc
     1381 caccatcctc gacgtgaacg agccggcggg cgcgcaggcc gtggcgcgac tcggcgatcg
     1441 cgccgcgttc atcaagaccg acgtgacgtc cgaggggact gtggatcaag cggttcaggt
     1501 cgcgacggcg tcgatgggcg ggctcagcct cgccgtcagc tgcgcaggtg tcggctggcc
     1561 gaagcgcatg gtcggcaagg aaggcccgat gcctggcgag ttcttccgca aggtgatcga
     1621 gatcaacctc gtcggcacgc tgctcgtcag caaggccgcg gcggccgcga tgcagaagaa
     1681 tgcgccgaat gccgggggcg agcgcggcgc catcgtcatg acggcgtcgg tggccgcatt
     1741 cgacggccag atcggccagg tcgcctactc ggcctccaag ggcggcgtgg taggcatgac
     1801 gctgccgatg gcacgcgagc tcgccgcatt cggcatccgc gtggtcacga tcgcgcccgg
     1861 cctgttcctg acgccgatga tggcggccct gccgcaggaa gcgcaggact cgctcggcaa
     1921 gcaggtgccc ttccccccgc ggctgggcaa tccggccgag tttgcctcgc tcgtacgcca
     1981 gatcgtggag aacccgatgc tgaacggcga gacgatccgc ctcgacgggg caatacgcat
     2041 ggcgccgcgc tgacgagccc ggttagagtt cgtgggtcgg cgcgcccggg agcgctgcgg
     2101 cgacgggtga ttctgagccg gcatcgagac ttgggggtca tagcatgagc gacatgaacc
     2161 aggcggccgt cgaacgcgat cagatggagt acgacgtggt cgtcgtgggc gccgggcccg
     2221 ccggcctcgc ctgcgcgatc cgcctgaagc agctcaagcc cgacctcaac gtctgcgtcc
     2281 tcgagaaagg cgcgagcatc ggcgcccaca tgctgtccgg ggccgtgctc gaaccgggcc
     2341 cgctcgacca gttgctgccc ggatggcggg acgagcacaa gggactgcgc gtgccggctg
     2401 gccgtgacga attcagcttc ctcacgaaga ccaagcgctt ccgcctgccc acgccgccgc
     2461 agatgaacaa ccacggcaac ttcatcgtgt ccgagggcgg cctcgtcgag tacctgggca
     2521 ccaaggcgga aggcctcggg tgcgacgtgt tcgccggctt cgccgccgcg tcgcccctct
     2581 tcgacgacca gggcgcggtc gtcggggctc gcatcggcga catgggactc gagaaggacg
     2641 gcgaacccgg gccgaatttc gcaccaggac ctgaaatacg cgccaggacc acgatcctcg
     2701 cggaaggctg ccgcggcagc atctccaaga tcctgatcaa gcgttacgga ctcgaccagg
     2761 gcaagtcgcc gcagacctac ggcctcggct tcaaggagct ttggcagctg ccaaaggggc
     2821 gcgttgaggc tggcctgatc cagcacacgg tcggctggcc gatggacgca gccacctacg
     2881 gcggcagctt catgtatcac ctcgaccagg accggctcta cgtcgggttc gtcgtgggac
     2941 tcgactacga ggacccgcgc ttccagccgt tcgaagcgta ccagcagttc aagaatcacc
     3001 cgagcctgcg cccgttattc gagggcggcg aggtcctggc ctacggcgca cgcaccatca
     3061 tcgagggagg atggcagtcg atgccgacgc tcgagatgcc gggcgcgctg ctgatcgggg
     3121 atgcgggagg cacgcttaac gtcacgaaga tcaagggcat tcaccaggcg atccgctcgg
     3181 cagtgctggc ggccgagcac atcgtcgagc atggccacac tgccggctac gaccagcgct
     3241 ggcgcgcgtc cgagggtggt cgcgaactgc acaaggtgcg caacatccgt cccgggttcc
     3301 gccacggcat gtgggtcggc ttggcgaatg gtgcactcga gacgctgacc gccggccact
     3361 cgccctggac gctgcagaac cacgccgacc acagcgcgtt gaagcgcctc gaccagtaca
     3421 cgtcgccgga tcgcggctgg gtcgagcgca gcctcccgcc gcgcgaccgg ctcgcagccg
     3481 tctatttcgc cggcaacacg cacgaggagg cgcaacccat ccacctcaag gtggccgaca
     3541 cgtcggtctg cgccacgcgc tgtgtcaccg agtacggcaa cccgtgccag aacttctgcc
     3601 cggcgcacgt ctacgagatg gtggacgacg gcgccggcgg aaagaaactg cagatcaacg
     3661 cgtcgaactg cgtgcactgc aaggcctgcg acatcaagga tcc
//
LOCUS       DAOMFD010000191         3693 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11763_length_3693_cov_31.281473, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000191 DAOMFD010000000
VERSION     DAOMFD010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3693)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3693
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11763_length_3693_cov_31.281473"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2271)
                     /locus_tag="PL152_09380"
     CDS             complement(<1..2271)
                     /locus_tag="PL152_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49537.1"
                     /translation="MSAAPELAAGQPAIDPEHPWLGLSSYTEETRGYFHGRDEEAAEL
                     ARRVQRKNLTVLFGQSGLGKTSLLRAGLVPRLRGEGYCPVYVRVDYSPESPPPAEQIK
                     QAIFKATAEAGHWTRPGTAVEGESLWEFLHHRGDLLRDASGRTVLPLLIFDQFEEIFT
                     LAQADDLGRQRAKQFIEDLADLVENRPPAALEARLDQDEKAAEDFDFARADYRILIAL
                     REDYLAHLESVKGIMPSITQNRMRLARMTGAQALSAVTGPGGRLVTQEVAESIVRFIA
                     GGSELANAEVEPSLLSLVCRELNNVRVAHGRREITADLLAGSRDTILSEFYERALADQ
                     PAGVRRVIEDELLTESGYRESLAEERVKKALAAAGAAPDALAKLVDRRLLRIEERLDV
                     RRVELTHDVLCSVVAASRDLRHEREARDEAERQLAAQREREAATRRALMRARTIAAVC
                     AVLMVIAAASAVFGWVSFKRARVADAQAQQARGDAEKLVGFLIEDFYAELAPTGRLDT
                     MGKLAHQAVSYYDGLPAGLMTPQTQVYRGMALIRQGQAQLGGKDIKGGTAKIAEARAL
                     FDKLYVADANSVAVAYGYALSMLAPFYSWGPGGGPGSKPHDLQVAADLLRPLAYAPDA
                     TRRVKITYADTLNFLSHSRDKKEEGIADCEEARKLLTGLGALDLTDLDATSAYADVTD
                     SEARHAMSLGRLDQAARLEQDVYELAEKALARRPGDLREMNNRYFAADMIGRLASSRQ
                     DYPETVRQYQTAPGTGA"
     gene            complement(2268..>3693)
                     /locus_tag="PL152_09385"
     CDS             complement(2268..>3693)
                     /locus_tag="PL152_09385"
                     /inference="COORDINATES: protein motif:HMM:NF024685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HQR49538.1"
                     /translation="HERDADRRLQDGCAMTAAMPLPENFDEDAWDDLLNYIEEKRVIP
                     IIGPDLLRVQTDRGLRPLYEWLAEKLAAKLQVDASVLPQPLTLNDVVCAYLGQRGRRE
                     EAYTRLRSIMREVEFEPPLALRQLAQITDFDLFVTTTFDPLLERAVNIERYGGQPTAE
                     VVAYAPNRVADLPAERGQLSRTVVYHLLGRLSASPTYVISDEDMLEFICALQSEHLTP
                     EKLFHELEHNHLLLIGSDFSNWLARLFLRMAKRKRLSDPRDVGEVLADDHTMKDERLV
                     AFLQQVSVRTRVYGGAESFVADLHARWIKRHGGAASPSASAAAPQRFLPPSREMPENA
                     IFISYAREDLPAVQKMKAAMDAAGLTTWFDLDRLEGGDDYDHKIRGNIGRCSFFMPVI
                     SATTQRRMEGYFRREWSYAVDRSRNIAEGAVFILPVCIDDTPEADALVPDRFKALHIT
                     RVPGGEPSLEFLGRLRDLFSGRRA"
BASE COUNT          534 a         1285 c         1262 g          612 t
ORIGIN      
        1 cgccccggta cccggtgctg tctggtactg gcggaccgtt tcggggtagt cctgacgcga
       61 gctcgcgagc cgcccgatca tgtctgcggc gaagtagcgg ttgttcatct ctcgcaggtc
      121 acccgggcgc ctggccagcg ccttctccgc cagctcgtag acatcctgct cgagccttgc
      181 agcctgatcg agtcgcccga gcgacatggc atgccgggct tccgaatcgg tcacgtccgc
      241 atacgccgac gtcgcgtcga ggtccgtgag atcgagtgcc cccagcccgg tgagcagctt
      301 ccgcgcctct tcgcagtcgg cgatgccctc ctccttcttg tccctcgagt ggctgaggaa
      361 attgagggta tcggcgtacg tgatcttgac gcgtcgcgtc gcgtcaggcg cgtaggcgag
      421 cggccgcaac agatcggccg ccacctgcag gtcgtgcggc ttcgatcccg gcccgccgcc
      481 cgggccccag gagtagaacg gcgccagcat cgacagcgcg tatccatagg ccacggccac
      541 gctgttggcg tcggcgacgt acaatttgtc gaacagcgcg cgtgcctcgg cgatcttcgc
      601 cgtaccgccc ttgatgtcct tgccgcccag ctgcgcctgg ccttggcgga tgagcgccat
      661 gccacggtag acctgggtct gcggcgtcat gagtcccgcg ggcaggccgt cgtagtacga
      721 cacggcctgg tgggcgagct tgcccatggt gtccagccgg cccgtggggg cgagctcggc
      781 gtagaagtcc tcgatcagga acccgaccag cttttccgca tcgccgcgtg cctgctgcgc
      841 ctgtgcatcc gccacccgcg cccgcttgaa gctgacccac ccgaacaccg cgcttgcggc
      901 cgcgatcacc atgagcacgg cgcagacagc ggcgatcgtc cgtgcgcgca tcagtgcacg
      961 gcgcgtcgca gcctcgcgct cacgctgggc cgcgagttgc cgctccgcct cgtcgcgcgc
     1021 ttcgcgctcg tggcgcaggt cgcggctcgc ggccaccacg ctgcagagca cgtcgtgcgt
     1081 gagctcgacg cgacgcacgt ccaggcgctc ctcgatgcgc agcagccggc ggtccaccag
     1141 cttcgcgagc gcgtcgggcg ccgcgccggc cgcggccagc gccttcttca cgcgctcctc
     1201 ggcgaggctc tcgcggtagc ccgactcggt gagcaactcg tcctcgatca cccgccgcac
     1261 tcccgcgggt tgatcggcca gtgcgcgctc gtagaactcg ctcaggatcg tgtcgcgcga
     1321 gcccgcgagc aggtcggccg tgatttcccg ccggccgtgc gccacgcgca cgttgttcag
     1381 ctcccgacac accaggctca gcagcgaggg ctcgacctcg gcattcgcga gctcgctgcc
     1441 accggcgatg aagcgcacga tcgactcggc cacctcctgg gtcaccaggc ggccaccggg
     1501 cccggtgacg gccgacaacg cctgcgcccc ggtcatgcgc gccaggcgca tgcggttctg
     1561 cgtgatcgag ggcatgatgc ccttcacgct ctcgaggtgt gcgagatagt cctcgcgcaa
     1621 ggcgatcagg atccggtagt cggcgcgcgc gaagtcgaag tcctcggcgg ctttctcgtc
     1681 ctgatcgagc cgcgcctcga gcgcggcggg cggccggttc tcgacgaggt cggcaaggtc
     1741 ctcgatgaac tgcttcgccc gctgcctgcc caggtcatcg gcctgtgcga gcgtgaagat
     1801 ctcctcgaac tggtcgaaga tgagcagcgg cagcaccgtc cgcccgctcg cgtcgcgcag
     1861 caggtcaccg cggtggtgca ggaattccca gagggattca ccttctaccg cggtcccggg
     1921 tctggtccag tgcccggcct cggccgtcgc cttgaagatc gcctgcttga tctgctcggc
     1981 cggtggcggg gactccgggg aatagtccac gcgcacgtag accgggcaat agccctcgcc
     2041 gcgcagccgg ggcacgagcc cggcccgcag cagcgacgtc ttgccgaggc cggattgccc
     2101 gaacagcacc gtgaggttct tgcgctggac gcggcgcgcg agttccgcgg cttcttcgtc
     2161 gcggccgtgg aaatagccgc gggtctcctc ggtatacgat gacaggccga gccacggatg
     2221 ctcgggatcg atcgcgggtt gcccggcggc gagctccggt gccgcgctca tgcgcgccgc
     2281 ccgctgaaca ggtcgcggag tcggccgagg aactcgaggc tgggctcgcc gcccggcacg
     2341 cgcgtgatgt gcagcgcctt gaagcgatcg ggcacgagtg cgtcggcctc gggcgtgtcg
     2401 tcgatgcaca cgggcaggat gaagaccgcg ccttcggcga tgttgcgcga acgatcgacc
     2461 gcatagctcc actcgcggcg gaaatagcct tccatccggc gctgggtcgt cgcggagatc
     2521 accggcatga agaagctgca gcgaccgatg ttgccgcgga tcttgtggtc gtagtcgtcg
     2581 ccgccctcga ggcggtcgag gtcgaaccac gtcgtcagtc ccgcggcatc catggctgcc
     2641 ttcatcttct ggaccgccgg caggtcctcg cgcgcgtagc tgatgaagat cgcgttctcg
     2701 ggcatctcgc gggacggcgg caggaaccgc tgtggcgccg cggccgatgc cgagggcgac
     2761 gccgcgccgc catgacgctt gatccagcgc gcgtgcaggt ccgccacgaa ggactcggcg
     2821 ccgccgtaca cgcgggtgcg cacgctcacc tgctgcagga acgccaccag ccgctcgtcc
     2881 ttcatggtgt gatcgtcggc aagcacttcg cccacgtcac gcggatcgga caggcgcttg
     2941 cgcttggcca tgcgcaggaa caggcgcgcc agccagttcg agaagtcgct gccgatcagc
     3001 agcaggtggt tgtgctcgag ctcgtggaac agcttctcgg gcgtcaggtg ctccgactgc
     3061 aaggcgcaga tgaactcgag catgtcctcg tcggagatca cgtaggtcgg tgatgccgac
     3121 agcctgccga gcaggtgata gaccacggtg cgcgagagct ggccgcgctc ggcgggcagg
     3181 tcggccaccc ggttcggcgc ataggcgacc acttcggccg tcggctgccc accgtagcgc
     3241 tcgatgttca ccgcgcgctc cagcagcggg tcgaacgtgg tggtgacgaa gaggtcgaag
     3301 tcggtgatct gcgccagctg cctgagcgcg agcggcggct cgaactcgac ctcgcgcatg
     3361 atcgagcgca ggcgggtgta cgcctcctcg cgcctcccgc gctggccgag gtaggcgcag
     3421 acgacgtcgt tgagcgtgag cggctgcggc aggaccgagg cgtcgacctg cagcttcgcg
     3481 gccagcttct cggccagcca ctcgtacagg ggacgaagcc cccgatccgt ctgcacgcgc
     3541 agcaggtcgg gaccgatgat cgggatgacc cgcttctcct cgatgtaatt gaggagatcg
     3601 tcccaggcat cctcgtcgaa attctcgggg aggggcattg cggccgtcat ggcgcacccg
     3661 tcctgcaggc gtcggtcggc gtcccgctca tgt
//
LOCUS       DAOMFD010000192         3692 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11769_length_3692_cov_37.457795, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000192 DAOMFD010000000
VERSION     DAOMFD010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3692)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3692
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11769_length_3692_cov_37.457795"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /gene="ribB"
                     /locus_tag="PL152_09390"
     CDS             complement(<1..538)
                     /gene="ribB"
                     /locus_tag="PL152_09390"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HQR49539.1"
                     /translation="MGSLNTTDEILEDIGRGRMVILMDDEDRENEGDLIMAASKVRPE
                     DVNFMARYGRGLICLTLTRDRCRQLRLPLMVSETDADQTTNFTVSIEAAEGVTTGISA
                     YDRAHTIRTAVAPDARPEHLRQPGHIFPLMAQPGGVLTRAGHTEAGCDLVRIAGLEPA
                     AVIVEILNEDGTMARRPQL"
     gene            complement(638..1234)
                     /locus_tag="PL152_09395"
     CDS             complement(638..1234)
                     /locus_tag="PL152_09395"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HQR49540.1"
                     /translation="MFTGIVQGVGRIRSVEPRGGDVTLWVETGPVALDDVAAGGSIAV
                     SGACLTATRLEGHAFAADVSRETLMLTTLGEAAAGRRVNIEKALAAGQPLGGHYVTGH
                     VDGIGRVVSRHDDARSVRMEFEVPAELARYVARKGSVCVDGVSLTVNGVAGRRFDVNL
                     VPHTLELTTLGDLRAGAAVNIEVDIIARYLERMLEAGR"
     gene            complement(1234..2352)
                     /gene="ribD"
                     /locus_tag="PL152_09400"
     CDS             complement(1234..2352)
                     /gene="ribD"
                     /locus_tag="PL152_09400"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HQR49541.1"
                     /translation="MTQFSSDDRRYMERALELAQNGLYSTPPNPAVGCVLVRWGQVVG
                     EGFHARAGGAHAEVAALADAGPAAAGATAYVTLEPCSHHGRTPPCVDALVAAQVSRVV
                     AAMRDPNPAVDGGGLARLESAGIATAVGLMADEAAEINRGFVSRATRGRPWITLKLGA
                     SLDGRTALANGASKWITGEAARADVQRLRARASAVVTGSGTVLADDPMLTVRDPRFEM
                     RGRRPLRVVLDTHLRTPPLSHVLSVPGPTLIFTQDADAAAGAALREAGATVERVAAGE
                     GGLDLQDVLSRLIARECNEVLVEAGPTVAGAFLRAGLVDEIVVYMAPVVLGSSARSLF
                     NLPALERMCDRCEFQWRDVERVGDDLRLTLRPRHREPD"
     gene            complement(2349..2870)
                     /gene="nrdR"
                     /locus_tag="PL152_09405"
     CDS             complement(2349..2870)
                     /gene="nrdR"
                     /locus_tag="PL152_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HQR49542.1"
                     /translation="MYCPFCAHEETKVIDSRLAGEGQQVRRRRECLKCGERFTTFESA
                     ELVMPRVVKSDATREPFDEAKLRSGMLKALEKRPVSSEALDAAVSHVCHNLRTLGERE
                     VPARLIGELVMDELHKLDEVAYVRFASVYRSFQDVDAFREEIERLRNRRPRTAEDVQL
                     GLWSGVEAGGDAK"
     gene            complement(2892..>3692)
                     /gene="glyA"
                     /locus_tag="PL152_09410"
     CDS             complement(2892..>3692)
                     /gene="glyA"
                     /locus_tag="PL152_09410"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HQR49543.1"
                     /translation="GEIDYAQVERLAHEHKPKLIIAGFSAYSRVIDWARFRGIADAVG
                     AWFLVDMAHVAGLVAAGVYPSPVPHAHVVTSTTHKTLRGPRGGLILARGNEEIAKKLN
                     ALIFPGTQGGPLMHVIAAKAVAFLEALQPEFAGYQKQVVSNARAMVAALQARGHKIVS
                     GGTDNHLFLVDLVSRSYTGKDADAALGRANITVNKNTVPNDPRSPFVTSGLRLGTPAV
                     TTRGFREPEVTQLAGWIADVLEDVGNESVIARTQAAVVDLCRRFPVYG"
BASE COUNT          537 a         1322 c         1281 g          552 t
ORIGIN      
        1 cgagctgcgg ccggcgcgcc atcgtgccgt cctcgttcag gatctcgacg atcaccgccg
       61 ccggttcgag gccggcgatg cgcacgaggt cgcaaccggc ctcggtgtgt cccgcgcgcg
      121 tgagcacccc gcccggctgc gccatgagcg gaaagatgtg tcccggctgg cgcaggtgct
      181 ccggacgcgc gtccggcgcc accgcggtgc ggatcgtgtg cgcccggtcg taagcggaga
      241 tcccggtcgt gacgccttcc gcggcctcga tcgacacggt gaagttcgtg gtctggtccg
      301 cgtccgtttc gctcaccatg agcggcaggc gcagctggcg gcaacggtcg cgggtgagcg
      361 tcaggcagat gagcccccgg ccgtagcggg ccatgaagtt cacgtcctcc ggccgcacct
      421 tggaggccgc catgatgagg tcgccctcgt tctcgcgatc ctcgtcgtcc atgaggatga
      481 ccatccggcc gcggccgatg tcctcgagga tctcgtccgt agtattgaga gagcccatgc
      541 gaaacagtct gtaatcgatt gttttactta agaacgaaag cccgcatccg tggcatgcct
      601 gggctgggga cgccagttta acaggcaggc gggtcgttca gcggccggcc tcgagcatcc
      661 tctcgaggta gcgcgcgatg atgtcgacct cgatgttcac cgccgccccg gcccgcaggt
      721 cacccagcgt ggtgagttcc agggtatgcg ggaccagatt cacgtcgaac cgtcggcctg
      781 ccacgccgtt cacggtcagg ctcacgccgt ccacgcagac ggagcccttg cgtgcgacgt
      841 agcgggcgag ttccgccggg acctcgaact ccatgcgcac cgaccgtgcg tcgtcatgcc
      901 gcgagaccac gcgaccgatg ccgtccacgt ggccggtcac gtaatggccg ccgagcggct
      961 gccccgcggc gagggccttc tcgatgttga cgcgccgacc ggctgcggct tccccgagcg
     1021 tggtgagcat gagcgtctcg cgcgacacgt cggcggcgaa tgcgtgcccc tcgagccggg
     1081 tcgcggtgag gcaggccccg ctcacggcga tgctgccgcc agccgctacg tcgtccagtg
     1141 ctacggggcc cgtctcgacc caaagggtca cgtcgccgcc gcgcggctcg acggagcgga
     1201 tgcgacccac gccctggacg atacccgtga acatcaatcc ggctccctgt gccgggggcg
     1261 cagcgtgagg cgcaggtcgt cgccgacacg ctcgacgtcc cgccactgga actcgcaccg
     1321 gtcgcacatc cgctcgagtg ccggcaggtt gaagaggctc cgcgcggaac tgccgagcac
     1381 gacgggcgcc atgtacacga cgatctcgtc gaccagcccg gcgcgcagga acgcgcccgc
     1441 gacggtcggc cccgcctcca ccagcacttc gttgcactcg cgcgcaatca ggcgcgacag
     1501 gacgtcctgc aggtccaggc cgccctcgcc ggccgccacg cgctcgacgg tcgcgccggc
     1561 ttcccgcaat gccgccccag ccgcggcatc ggcatcctgc gtgaagatca gcgtcgggcc
     1621 cgggaccgac aggacgtgcg agagcggcgg cgtgcgcagg tgagtgtcga gcacgacccg
     1681 gagcggtcgc cggccccgca tttcgaagcg cgggtcgcgc acggtcagca tcgggtcgtc
     1741 ggcgagcacc gtgccgctgc cggtgacgac cgcggacgcg cgcgcgcgca atcgctgcac
     1801 gtcggcacgc gcagcctctc ccgtgatcca cttgctcgcg ccgttggcaa gcgcggtgcg
     1861 gccgtcgagg ctcgcgccca gcttgagggt gatccacggc cggccacgcg tcgcgcgact
     1921 cacgaagccg cgattgatct ccgccgcctc gtcggccatg aggccgaccg ccgtggcgat
     1981 gccggcagac tcgagtcgcg cgagcccgcc gccgtccacg gcgggattcg gatcccgcat
     2041 ggcggcaacc actcgcgaca cctgcgccgc gacgagcgcg tccacgcaag gcggcgtgcg
     2101 gccatggtgc gaacacggct cgagggtgac gtatgcagtc gcgcccgccg cggccggacc
     2161 ggcatccgcg agcgcggcga cctccgcgtg cgcgccaccg gcacgcgcgt ggaagccttc
     2221 gccgacgacc tgcccccaac gcacgaggac gcaccccacg gcggggttgg gcggtgtcga
     2281 gtacagccca ttctgcgcga gttccagcgc gcgctccatg tagcgccggt cgtcgctgga
     2341 aaactgggtc acttggcgtc gcccccggcc tcgacccccg accagaggcc gagctgcacg
     2401 tcctcggccg tccgcggccg ccggttgcgc aggcgctcga tctcctcgcg gaaggcgtcc
     2461 acgtcctgga agctgcggta caccgaggcg aagcggacgt aggcgacctc gtcgagcttg
     2521 tgcagctcgt ccatcaccag ttcgccgatc aggcgcgccg gcacctcgcg ttcgcccagc
     2581 gtgcgcaggt tgtggcacac gtggctcacc gccgcgtcca gggcttcgct cgagaccggc
     2641 cgcttctcga gcgccttcag catgccggag cgcagcttgg cctcgtcgaa gggttcgcgc
     2701 gtggcgtcgc tcttgaccac ccgaggcatc accagctccg ctgactcgaa ggtggtgaag
     2761 cgctcgccgc acttcaggca ctcgcgacga cgacggacct gctggccctc gcccgcgagt
     2821 cgcgagtcga tgaccttggt ctcctcgtgc gcgcagaacg ggcagtacat gagcggtccg
     2881 cccgggcccc gtcagccgta gaccgggaag cgtcggcaga gatccaccac tgccgcctgc
     2941 gtgcgtgcaa tgacggattc gttgccgacg tcctcgagca cgtcggcgat ccagccggcc
     3001 agctgggtga cctcgggctc gcggaaaccg cgcgtcgtca cggcgggcgt gccgagtcgc
     3061 agcccgctcg tcacgaacgg cgagcgggga tcgttcggca cggtgttctt gttgaccgtg
     3121 atgttggcgc ggccgagggc cgcgtcggcg tccttgccgg tataactgcg cgagaccagg
     3181 tcgacgagga acaggtggtt gtcggtgccg cccgagacga tcttgtggcc tcgcgcctgc
     3241 agggcagcga ccatcgcccg cgcattggac accacctgct tctggtaccc ggcgaactcg
     3301 ggctgcagcg cctcgaggaa cgcgaccgcc ttggcggcga tcacgtgcat gagcggcccg
     3361 ccctgcgtgc cggggaagat gagcgcattc agcttcttgg cgatctcctc gttgccccgc
     3421 gcgaggatga ggccgccacg cggaccgcgc agcgtcttgt gcgtggtgga cgtgacgacg
     3481 tgggcgtgcg ggacggggct cggatacacg cctgccgcga cgaggcccgc gacgtgcgcc
     3541 atgtcgacca ggaaccaggc gccgaccgca tcggcgatcc cgcggaagcg tgcccagtcg
     3601 atgacgcgcg aataggccga gaagccggcg atgatgagct tcggcttgtg ctcgtgcgcg
     3661 aggcgctcga cctgcgcgta gtcgatctcg cc
//
LOCUS       DAOMFD010000193         3689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11790_length_3689_cov_37.953770, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000193 DAOMFD010000000
VERSION     DAOMFD010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11790_length_3689_cov_37.953770"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(11..778)
                     /locus_tag="PL152_09415"
     CDS             complement(11..778)
                     /locus_tag="PL152_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HQR49544.1"
                     /translation="MPHSEFLATALEAARAAADVIRHYYQSNLAITIKADKSPVTVAD
                     VETEQAIRAILGAKFPQHGFYGEETGTSALDAEYVWIVDPIDGTKAFVREYPMFSTQI
                     ALMHRGRLVVGVSSAPAYGELAYGEVGVGAWLNDRPIRVSEVSEVEDAALSTGNLKSL
                     ATGPQWPAFGRLVGRLGRIRGYGDFLQYHLLAAGRIDAVVESDVNVLDVGACAVIVEA
                     AGGRFTDLDGGPFGLASRTVLATNGRLHAPVLAALRA"
     regulatory      923..1135
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1238..3148
                     /locus_tag="PL152_09420"
     CDS             1238..3148
                     /locus_tag="PL152_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HQR49545.1"
                     /translation="MSIRNLACAAALVGVSLSAASRASEPGLTDEEVVVTATRLPMPQ
                     DEVLATTLVIDRDAIEQSGAGDAGDILRFHAGLDLARNGGPGQTTAIFIRGADSNHTL
                     VMVDGVRINPGTIGLAPLQSIAPDSIERIEVVKGPRSSLWGTDAIGGVINVITRRGTG
                     DFWTAEAGYGAYDTQQASLNGGLSLGLAQLGLGVNWIDSQGFPTRTDDDVDRGFHNLS
                     GTASLRGDVGPADVELSYWRASGTTQYSDFYFAPVDQDFATSSLALNAAVPLGDALQA
                     SFTASHFDDSIDQNQSTDFLRTRRDTVDAQIDWRRATQTWSAGAMATREDASSRSYGD
                     EFDSVTHAVNLYVQDQLEIGPHRALAAVGYTDHETAGNAWTWNLEYGYTLREATLLYA
                     LGGTGYRVPDATDRYGYGGNPGLAPERSLNLEAGIRQRFSATQSASLAWFRNDIHDLI
                     EFVVQSWDPFVGQNENVDEARIEGAEASYRYESGPWQLLVEAIHQDPRNLTTGARLLR
                     RADDSLTVGVTRRIGQFDLGLDMLATSDRKDYGDTGLVTLASYVLANLTARWQPTPAL
                     TVSARVENLLNEHYELAYDYNTPARGIYVSVSYAMKAVGPPAAVTASGTGDSAPPRGA
                     YSAAAGSTRTTR"
     gene            3148..>3689
                     /locus_tag="PL152_09425"
     CDS             3148..>3689
                     /locus_tag="PL152_09425"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HQR49546.1"
                     /translation="MGHRLTRIYTRTGDDGTTGLGDGERVPKEHLRVEAYGTVDEANS
                     AVGMVLAVPGLPVRVTDCLTSVQHQLFELGGELCMPGHRAITGDDVERLERELDDFNA
                     GLPPLKEFILPGGGPASAACHLARTVTRRAERRVWSLSRVDTVAREVPMYLNRLSDLL
                     FVIARVLARHESGSEVLWSKR"
BASE COUNT          583 a         1325 c         1275 g          506 t
ORIGIN      
        1 ggctgaccgg tcaggcgcgc agcgccgcca gcaccggcgc gtgcaggcgg ccgttcgtcg
       61 ccagcaccgt gcgactggcg agcccgaacg ggccgccgtc caggtcggtg aaccggccgc
      121 ccgccgcctc cacgatgacc gcgcaggcac ccacgtcgag cacgttcacg tccgactcga
      181 ccaccgcgtc gatgcggcca gccgcgagaa gatggtactg gaggaaatca ccgtagcccc
      241 ggatgcggcc gagccggccg acgagccggc cgaaggccgg ccactgcggc ccggtggcca
      301 gggacttcag gttgcccgtc gacaatgcgg cgtcctcgac ctcgctcacc tcgctcacgc
      361 ggatgggccg gtcgttgagc caggccccga ccccgacctc gccataggcc agttccccgt
      421 acgccggcgc cgacgacacc ccgaccacca gccgaccgcg gtgcatgagc gcgatctgcg
      481 tcgagaacat cgggtattcc cgtacgaagg ccttggtgcc gtcgatcggg tcgacgatcc
      541 acacgtactc cgcgtcgagc gcgctcgtgc cggtctcttc gccgtagaac ccgtgctgcg
      601 ggaatttcgc gcccaggatc gcgcggatcg cctgctcggt ctcgacgtcc gccaccgtga
      661 ccggcgactt gtcggccttg atcgtgatcg cgaggttgga ctggtagtag tgacggatga
      721 cgtcggcggc ggctcgcgcg gcctcgaggg cggtggcgag gaattctgag tggggcatcg
      781 tgctcgggcg gcagtggaac ggcgcgacat tatcgccgtt tgcgtcgcgg gccgccgact
      841 gccggctgcg gcaagccggg cgacgaccac ggacggcgga ctggcggagt tttcctcctt
      901 gacgccatcg gccgcgctcc ctatcgtgcc cgcaccgcaa ggtgatcccg cgcgatgcgc
      961 gggagtacgg gaagccggtc ccattccggc gctgcccccg caacggtagt cgagtcgagc
     1021 ccgcatccgc cggatggtcc ggcacccact gcgcaaggcg cgggaagggg atgcaggcgg
     1081 gatcccaggg atccactcga gagcccggag accggcctcg cggacgagcc cagctacgcg
     1141 gagggcgagc ggccgggatt gcgccgcgcg cgctcacccg acccaccgat ctccccggct
     1201 ggaccacgga gccctgcggg cgtgcatggg agtggcggtg agcattcgaa acctggcctg
     1261 cgcggccgcc ctggtcgggg tgtcgctgtc ggcggcgtca cgcgcaagcg aacccggcct
     1321 gaccgacgag gaagtggtcg tcaccgcgac gcggctgccg atgccgcagg acgaggtgct
     1381 cgcgacgacc ctggtgatcg accgtgacgc gatcgagcag agcggcgccg gcgacgccgg
     1441 cgacatcctg cgcttccacg ccgggctcga cctcgcacgc aacggcggtc ccggccagac
     1501 caccgcgatc ttcatccgcg gcgcggacag caaccacacg ctcgtcatgg tggacggcgt
     1561 gcgcatcaat cccggcacga tcgggctcgc accgctgcag agcattgctc cggactcgat
     1621 cgagcgcatc gaggtggtca agggcccgcg ctcctcgctc tggggcacgg atgcgatcgg
     1681 cggcgtgatc aacgtcatca cacgccgcgg cacgggcgat ttctggaccg ccgaggcagg
     1741 ctacggcgcc tacgacacgc agcaggccag tctgaatgga gggctgagtc ttgggctcgc
     1801 gcagctgggg ctcggcgtca actggatcga cagccagggc ttcccgacgc gcaccgacga
     1861 cgacgtcgat cgcggcttcc acaacctgag cggcaccgcg tcgctgcgcg gcgacgtcgg
     1921 gccggccgac gtcgagctga gttactggcg cgcgtcgggc accacgcagt actccgactt
     1981 ctacttcgcg ccggtggacc aggacttcgc gacgtcttcg ctcgcgctca acgcggccgt
     2041 gccgctcggc gatgcgctgc aggcgagctt caccgccagc catttcgacg acagcatcga
     2101 ccagaaccag tcgacggact tcctgcggac gcgccgcgac accgtcgatg cgcagatcga
     2161 ctggcgacgc gcgacgcaga cgtggagcgc cggcgcgatg gcgacgcggg aggacgcgag
     2221 cagccgttcg tacggggacg agttcgattc cgtcacccac gccgtgaacc tgtacgtgca
     2281 ggaccagctc gagatcggcc cgcaccgggc gctcgcggcc gtcggttaca ccgaccacga
     2341 gacggccggc aatgcctgga cgtggaatct cgagtacggg tacacgctgc gcgaggcgac
     2401 gctgttgtac gcactcggcg gcaccggcta ccgcgtgccc gacgccacgg accgctacgg
     2461 ctacggcggc aacccgggcc ttgcgcccga gcgctcactc aatctcgagg ccgggatccg
     2521 ccagcgattt tccgccacgc agtccgcgtc gctcgcctgg ttccgcaacg acatccacga
     2581 cctgatcgag ttcgtcgtgc agtcgtggga cccgttcgtc ggccagaacg agaacgtcga
     2641 cgaggcccgc atcgagggag cggaggcttc gtatcgctac gagtctggtc cgtggcagct
     2701 gctggtcgag gcgatccacc aggacccgcg caacctcacc accggcgcgc gactcctgcg
     2761 ccgggccgac gacagcctca cggtcggcgt cacgcgccgg atcggacagt tcgacctcgg
     2821 gctggacatg ctcgcgacca gcgaccgcaa ggactacggc gataccggcc tggtgacgct
     2881 cgcgagctac gtgctcgcca acctcaccgc gcgctggcag ccgacaccgg cgctcacggt
     2941 ctcggcccga gtggagaacc tgctgaacga gcactacgaa ctcgcctacg actacaacac
     3001 cccggcccgc ggcatctacg tgtcggtcag ctacgccatg aaggccgtcg gccctcccgc
     3061 agcggtgacc gcctccggca cgggcgacag cgcgccgccg cgcggggcat actccgcagc
     3121 cgctggctcc acccggacga cacgctgatg ggacaccgac tgaccaggat ctacacgcgc
     3181 accggcgacg acggcacgac gggcctcggc gacggcgagc gcgtgccgaa ggaacacctg
     3241 cgcgtcgagg cctacggcac cgtggacgag gcgaacagcg ccgtgggcat ggtactcgcc
     3301 gtgccgggcc tgccggtgcg agtcacggac tgcctgacgt ccgtgcagca ccagctgttc
     3361 gaactcggcg gcgagctgtg catgcccggc caccgggcga tcaccggcga cgacgtggag
     3421 cgcctcgagc gcgaactcga cgacttcaat gccggactgc cgccgctcaa ggaattcatc
     3481 ctgccgggcg gcggtcctgc gtcggcggcg tgccacctcg cgcggaccgt gacccgtcgc
     3541 gcggagcggc gcgtgtggtc gctgtcgcgg gtcgataccg tggcccgcga agtgcccatg
     3601 tacctgaacc ggctgtcgga cctgctgttc gtgatcgcgc gcgtgctcgc gcgccacgag
     3661 tccggcagcg aggtgctctg gagcaagcg
//
LOCUS       DAOMFD010000194         3677 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11856_length_3677_cov_34.112645, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000194 DAOMFD010000000
VERSION     DAOMFD010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3677)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3677
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11856_length_3677_cov_34.112645"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="PL152_09430"
     CDS             <1..1152
                     /locus_tag="PL152_09430"
                     /inference="COORDINATES: protein motif:HMM:NF017727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HQR49547.1"
                     /translation="LALASLWWVGHLVARYTGFPLFALQVAVAPIWAHSFLTLFTMLP
                     AFMFGFLFTTFPRWMNGPQVPRAAYVPTALLLVAATVTWLAGVHTGRALQLAGVLLAV
                     AGLATGLTALLRVLIDAEQVVSHAVVAIIALAVGIVAAAGFAYGTWNSDDFAAHFAVR
                     TALWGFLLPIFFAVCHRMVPFFSQNAIPGYVAWRPRWVLVAVVALAYARLLLGTVGAF
                     EALVVVDLATTALTAACAWRWSSRGARGNPLLWVLYAAIAWLPIAMALQTVRDASFVL
                     TGTWALGRAPIHALGIGYFGGLLVAMVTRVTLGHSGRPLRMDRTALACFAGVQFAALA
                     RVCSEVVTTPGMVRDLLLGSAVLWLAAFGTWVVRSLGIYLSPRIDGKPG"
     gene            1149..1541
                     /locus_tag="PL152_09435"
     CDS             1149..1541
                     /locus_tag="PL152_09435"
                     /inference="COORDINATES: protein motif:HMM:NF016161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB2 family protein"
                     /protein_id="HQR49548.1"
                     /translation="MIEYYLPLRAVHMTCAALTISLFFARGVLMLLGSPAVLHPVLRW
                     TSVAIDTVLLTSALMLTTIIQQYPGTAPWLTTKVVLLVVYIVLGSVALKRGRTRAIRG
                     VAFVAALATVAFLVTVALTHQPLGFLAR"
     gene            1610..2305
                     /locus_tag="PL152_09440"
     CDS             1610..2305
                     /locus_tag="PL152_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HQR49549.1"
                     /translation="MPNAENLVPLLRGHHLFAGMSPEQLQRVLAAAHVDDYEAGHLLF
                     DRGQPAQHFYIVVDGQVNLVLYSKTGEEKIVDILGPGSSFAEAVMFMEGSAYPVSAVA
                     AARSTVVRFQNREYLSILRESPATCLRMLGHLSQRLHMRIREIEYLTLESATHRLVRL
                     LESRLPADATGPCEINLVESRQDLASRLAMKPETLSRILRHLSDSGAIVVRGRQLQVP
                     DRKRLLAVLDSVP"
     gene            2425..>3677
                     /locus_tag="PL152_09445"
     CDS             2425..>3677
                     /locus_tag="PL152_09445"
                     /inference="COORDINATES: protein motif:HMM:NF014312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome D1 domain-containing protein"
                     /protein_id="HQR49550.1"
                     /translation="MSNSIVRSPITLALAVSGCLAFGAAAWAQQPSPASSTPQVHQAE
                     VAGAASLEAQMASGKKVYDTICYACHQPDGKGLPGAFPPLAGSDYLLGNPDRAAKVVI
                     NGLTGEVEVNGVKYNSVMPAMTQLSDQEIADALTYAMNSWGNKGGQVSVTNVAAARAK
                     AEATPKKAESPTQHPNVQAELKYQGAPSPVGAAGAEVLVKPGAPPMTEAEFTQARQIY
                     FERCAGCHGVLRKGATGKPLTPDITQKKGTEYLKVFINQGSPAGMPSWGKSGELTAEQ
                     VDLMARYVQNEPPQPPEFGMKDMQASWKVLVPVKDRPKTQQNKYNLQNIFAVTLRDAG
                     EVALIDGDTKQIINTIKTGYAVHISRLSSTGRYVYTIGRDAKIDLIDLYMPTPAVVAE
                     IKVGLEARSVETSKYKGYEDKYAIAG"
BASE COUNT          553 a         1273 c         1233 g          618 t
ORIGIN      
        1 ctcgcgctcg ccagcctctg gtgggtcggg catctcgtcg cccgctacac cggcttcccg
       61 ctcttcgcgc tgcaggtcgc ggtcgcgccg atctgggcgc actcgttcct gacgctgttc
      121 acgatgctgc ctgcgttcat gttcggcttc ctgttcacga cgtttccgcg ctggatgaac
      181 gggccgcagg ttccgcgtgc cgcgtacgtc cccacggcgc tgctgctggt cgccgcgacc
      241 gtgacgtggt tggccggcgt gcacaccggt cgtgcgctcc agctggccgg ggtgctgctc
      301 gcggtggcag ggctggcgac cgggctgact gccttattgc gggtgctgat cgacgccgag
      361 caggtcgtat cgcacgcggt ggtcgcgatc atcgcgctcg cggtggggat cgtcgcggct
      421 gcgggcttcg cctacggcac ctggaacagc gacgatttcg ccgcgcattt cgccgtgcgc
      481 accgcgctgt ggggattcct gctgccgatc ttcttcgcgg tctgccaccg gatggtcccg
      541 ttcttctcgc agaacgcgat ccccgggtac gtcgcgtggc gcccgcgctg ggtgctggtc
      601 gccgtcgtcg ccctcgcgta tgcccgcctg ctgctcggca ccgtgggcgc attcgaggcg
      661 ctcgtcgtgg tcgacctggc gacgacggcg ctgacggcag cctgtgcctg gcgctggagc
      721 tcacgaggcg ctcgcggcaa cccactgctg tgggtgctgt atgccgcaat cgcgtggctg
      781 ccgattgcca tggcgctgca gacggtgcgg gatgccagct tcgtgctcac cgggacgtgg
      841 gcgctcggcc gcgcgccgat ccacgcgctg ggtatcggct acttcggcgg gctgctggtc
      901 gccatggtga cgcgggtcac cctgggtcac tcgggccggc cgttgcgcat ggaccgcacg
      961 gcgctcgcgt gctttgccgg cgtgcagttc gccgcgctgg cccgggtttg cagcgaggtc
     1021 gttacgacgc ccgggatggt gcgcgacctg ctgctcggca gcgccgtgct gtggctggcc
     1081 gcgttcggca cgtgggtcgt gcgcagcctc ggcatctacc tgtcgccgcg catcgacgga
     1141 aagcccgggt gatcgagtac tacctgccgc tgcgggcggt gcacatgacc tgcgccgcgc
     1201 tgacgatctc gctgttcttc gcacgcgggg tgttgatgct gctgggttcg ccggcggtgc
     1261 tgcatccggt cctgcgctgg accagcgtcg cgatcgacac ggtcctgctc acctcggccc
     1321 tcatgctgac gacgatcatc cagcagtatc ccggcaccgc tccctggctc acgaccaagg
     1381 tggtgctcct cgtggtgtac atcgtcctcg gcagtgttgc gctcaagcga ggccgaaccc
     1441 gggcgatccg gggcgtcgcg ttcgtcgccg ccctcgccac cgtcgcgttc ctggtgaccg
     1501 tggcgctgac ccaccagccg ctcggcttcc ttgcccgctg agacaggggg gcgttcggcc
     1561 ggcagcgatg ccctttccac cagcgcgtgc ggctgctagc gtcccgtgca tgcccaacgc
     1621 cgaaaacctc gtacccctcc tccgcggcca tcacctgttc gcggggatgt cccccgaaca
     1681 gctgcagcgc gtgctcgccg cggctcacgt ggatgactac gaggcagggc acctgctgtt
     1741 cgaccgcggc cagcccgccc agcacttcta catcgtcgtg gacggacagg tgaacctggt
     1801 cctgtattcc aagaccggcg aggagaagat cgtggacatc ctgggcccgg gctcgagctt
     1861 cgccgaggca gtgatgttca tggagggctc ggcctatccc gtctccgccg tcgcagccgc
     1921 gcgctcgacg gtggtccggt tccagaaccg ggaatacctg tcgatcctgc gcgagagccc
     1981 cgcgacgtgc ctgcgcatgc tggggcacct cagccagcgc ctgcacatgc gcatccggga
     2041 gatcgagtac ctgacgctcg agagcgcgac ccaccgcctg gtgcggctgc tcgagtcgcg
     2101 gctgcctgcc gacgccacgg gaccgtgcga gatcaacctc gtcgagtcgc gccaggatct
     2161 cgcctcgcgc ctcgcgatga agcccgagac cctgtcgcgg atcctccgcc acctgtcgga
     2221 ctccggcgcg atcgtcgtgc gcggacgcca gctgcaggtg cctgaccgga aaagacttct
     2281 ggcggtgctc gacagcgttc cctgagcgcc ggtcccgact tgatcgggat caatgcgaat
     2341 atcgccgcag gcccctatcg tccgagactg ttgcggtggt tgcgactgcg gccatcccgg
     2401 ttacagacca tgggagcact ccacatgtcg aattcgatcg tccgatcgcc gatcacgctg
     2461 gccctggcgg tgtcgggatg cctggccttt ggcgcggcgg cctgggcaca acagcccagc
     2521 cctgcatctt ccacgccgca ggtgcaccag gcggaagtcg ccggcgccgc gtcgctcgag
     2581 gcgcagatgg ccagcggcaa gaaggtatac gacacgatct gttacgcctg ccaccagccg
     2641 gacggcaagg gcctgccggg cgcgttcccg ccgctggccg gctcggacta cctgctcggc
     2701 aatccggatc gcgcggccaa ggtcgtgatc aacggcctca cgggcgaagt cgaggtcaat
     2761 ggcgtcaagt acaactcggt catgcccgcg atgacacagt tgtccgacca ggaaattgcc
     2821 gacgccctga cctatgcgat gaactcgtgg ggcaacaagg gcggacaggt gtcggtgacc
     2881 aacgtcgccg cggcacgcgc caaggcggag gcgacgccga agaaagcgga gtccccgacc
     2941 cagcacccca acgtccaggc cgaactgaag taccagggag cgccgtcccc cgttggcgcg
     3001 gcgggcgccg aagtgctcgt caagccgggc gccccgccga tgaccgaggc ggagttcacc
     3061 caggcgcggc agatctactt cgagcgctgc gcaggctgcc acggcgtact gcgcaaaggc
     3121 gcgacgggca aaccgctcac gccggacatc acccagaaaa aaggcaccga gtacctcaag
     3181 gtcttcatca accagggctc cccggccggc atgccgagct ggggcaagtc cggcgaactc
     3241 acggccgagc aggtggacct gatggcgcgc tacgtgcaga acgagccgcc gcagccgccc
     3301 gagttcggca tgaaggacat gcaggcgagc tggaaggtgc tggtgccggt caaggaccgc
     3361 ccgaagaccc agcagaacaa gtacaacctg cagaacatct tcgcggtcac cctgcgtgat
     3421 gcaggcgaag tcgcgttgat cgacggcgac accaagcaga tcatcaacac gatcaagacc
     3481 ggctacgcgg tgcacatctc gcgcctgtcg agcaccggcc gctacgtgta cacgatcggc
     3541 cgcgacgcca agatcgacct gatcgacctc tacatgccga cacccgcggt ggtcgccgag
     3601 atcaaggtgg gcctcgaggc gcgctcggtc gagacctcga agtacaaggg ctacgaggac
     3661 aagtacgcga tcgccgg
//
LOCUS       DAOMFD010000195         3661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_11954_length_3661_cov_36.133666, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000195 DAOMFD010000000
VERSION     DAOMFD010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3661
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11954_length_3661_cov_36.133666"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..767)
                     /gene="tdh"
                     /locus_tag="PL152_09450"
     CDS             complement(<1..767)
                     /gene="tdh"
                     /locus_tag="PL152_09450"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013343673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008743 - L-threonine 3-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HQR49551.1"
                     /translation="MKALVKSQRAPGIWLDDVPEPKVGHNDVLIRIRKTAICGTDIHI
                     YNWDHWAQKTIPVPMHVGHEYCGEIVAIGSEVQGFAVGDRVSGEGHITCGYCRNCRAG
                     RRHLCRNTVGVGVNRPGCFAEYLAIPAFNAFKLPDAITDEIASILDPFGNATHTALSF
                     NLVGEDVLITGAGPIGIMATAIARHVGARHVVVTDVNDYRLGLARAMGASRAINVTRE
                     SLDDTMKSLGMVEGFDVGLEMSGNPKAFRDLLRTMHHG"
     gene            complement(792..1982)
                     /locus_tag="PL152_09455"
     CDS             complement(792..1982)
                     /locus_tag="PL152_09455"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF005394.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HQR49552.1"
                     /translation="MDPNFLARLAGQCDELKASGLYKPERVLGSPQQPVVRLADGREL
                     INLCANNYLALANHPSIREAAHRALDRYGYGMASVRFICGTQTVHKELEARISAFLGT
                     DDTILYSSCFDANGGLFETLLDEQDAVISDALNHASIIDGVRLCKAQRLRYANDDLDE
                     LETRLKEAAGARVRLIATDGVFSMDGVIARLKDICDLADRYGALVMVDDSHATGFVGK
                     GGRGTPEYREVMGRVDILTGTLGKALGGASGGYTSARKEIVAWLRQRSRPYLFSNSIP
                     PVIAAATLRVLDLVEQGDELRAQLRRNTDHFRSRMAAAGFRIAAGEHPIAPVMLGDAA
                     LAARFAEQLLDRGVYVIGFSYPVVPMGKARIRTQMCAGHTLEQLERAIEAFVAVGREL
                     RVIA"
     gene            2031..2459
                     /locus_tag="PL152_09460"
     CDS             2031..2459
                     /locus_tag="PL152_09460"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HQR49553.1"
                     /translation="MSRKSAHDEDASPGMDAGAGASLRIDKWLWCARFYRSRGLAQEA
                     VEGGHVQVNGDRVKASRQVKVGDSLRIVRGQERFDVVVTGIPGRRGPATEARGHYAET
                     AESEAARAVAREFNRLTAPAHSGRPDKRERRDLLKHFKNR"
     gene            2456..3226
                     /locus_tag="PL152_09465"
     CDS             2456..3226
                     /locus_tag="PL152_09465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HQR49554.1"
                     /translation="MTASLSSRSTRLFIGLTAFFVGNAIVAEFVGVKIFALETTFGLQ
                     PFGWNLFGQSGSLSFTAGVLIWPIVFIMTDVINEYFGRRGVRLISFAAAGMITYAFFF
                     AYAAMHLAPAAWWVTVNSARGVPDMQAAYDTIFGQGLWTIGGSLAAFLVGQLVDVAIF
                     HRIRESTGERWIWLRATASTAVSQLVDSFLVLYVAFVLGPQQWPVSLFLAVGTVNYCY
                     KMVMAVLLSPLIYVARNGIERYLGHAEAARLKAEAAAE"
     gene            3223..>3661
                     /locus_tag="PL152_09470"
     CDS             3223..>3661
                     /locus_tag="PL152_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HQR49555.1"
                     /translation="MTGQGCGPLLTLREHERLLAGRERGSAVVECSLDLGRSTDLVKA
                     GPDAWTWRGVQFPYLPTCRERAVYYWEDGQFRLAARFGRSLVKLVPTEWGAPTFEIDG
                     IKMLPTDASSPYEDARRKIDLVRVRGRAVLDTCGGLGYYAAWCL"
BASE COUNT          536 a         1195 c         1312 g          618 t
ORIGIN      
        1 ccgtggtgca tggtgcgcag caggtcgcgg aaggccttcg gattgcccga catctcgagc
       61 ccgacgtcga agccctccac catgccgagc gacttcatgg tgtcatcgag cgattcgcgc
      121 gtgacgttga tggcgcgcga ggcgcccatc gcgcgcgcca gtcccaggcg gtagtcgttc
      181 acgtccgtga ccacgacgtg ccgcgctccc acgtggcggg caatggccgt cgccatgatg
      241 ccgatcgggc cggcgccggt gatgagcacg tcctcgccca cgaggttgaa cgacagggcc
      301 gtgtgcgtcg cgttgccgaa cggatcgagg atcgacgcga tctcgtcggt gatcgcatcc
      361 gggagcttga aggcgttgaa cgcggggatc gcgaggtatt cggcgaagca gcccgggcga
      421 ttgacgccga cgccgacggt gttgcggcac aggtgccgcc gcccggcgcg gcagttgcgg
      481 cagtagccgc aggtgatgtg tccttcgccg gacacgcgat cgccgacggc gaagccctgc
      541 acctcgctgc cgatcgccac gatctcgccg cagtactcgt ggccgacgtg catgggcacc
      601 gggatcgtct tctgcgccca gtggtcccag ttgtagatgt ggatgtctgt gccgcagatc
      661 gccgtcttgc ggatccggat cagcacgtcg ttgtggccga ccttcggttc cgggacgtcg
      721 tcgagccaga tcccgggggc gcgttgtgac ttgaccagtg ccttcatgtg ccggtgtctc
      781 cgttgttctt gttacgcgat cacgcgcagt tcccgcccga cggcgacgaa cgcctcgatc
      841 gcgcgttcga gctgctcgag cgtgtgaccc gcgcacatct gcgtgcggat gcgagccttg
      901 cccatcggca cgaccgggta ggagaagccg atgacgtaca ccccgcggtc gagcagttgc
      961 tcggcgaagc gggcggcgag ggcggcgtca ccgagcatca cgggcgcgat cggatgctcg
     1021 ccggccgcga tgcggaatcc ggcggcggcc atgcgactgc ggaagtggtc ggtgttgcga
     1081 cgcagttgag cgcgcaactc gtcgccctgc tcgacgaggt ccagcacgcg cagggtcgcc
     1141 gcggcaatga ccggcgggat gctgttcgag aacaggtagg gtcgcgagcg ctgccgcagc
     1201 cacgcgacga tctccttgcg cgccgacgtg tacccgcccg aggcgccgcc gagggccttg
     1261 cccagcgtgc cggtcaggat gtcgacgcga cccatgactt cgcgatactc gggcgtgccc
     1321 cggccgccct tgccgacgaa gcccgtggcg tgggagtcgt ccaccatcac gagcgcgccg
     1381 tagcggtcgg cgagatcgca gatgtccttg agccgggcga tgacgccgtc catcgagaac
     1441 acgccgtcgg tggcgatcag ccggacccgt gcgccggccg cctccttcag gcgcgtctcg
     1501 agttcgtcga ggtcgtcgtt cgcgtaccgc agtcgctgcg ccttgcagag gcgcaccccg
     1561 tcgatgatgc tcgcgtggtt caacgcgtcg ctgatcacgg cgtcctgctc gtcgagcagc
     1621 gtctcgaaca ggccgccgtt cgcgtcgaag caggacgagt agagaatcgt gtcgtcggtg
     1681 ccgaggaagg cggaaatgcg tgcctcgagt tccttgtgca ccgtctgcgt cccgcagatg
     1741 aagcgcacgg acgccatgcc gtagccatag cggtcgagcg cgcggtgggc cgcctcgcgg
     1801 atggacgggt gattggcgag cgcgaggtag ttgttggcgc acagattgat cagctcgcgg
     1861 ccgtcggcga ggcgcacgac cggttgctgc ggcgagccga gcacgcgctc gggcttgtag
     1921 aggccggatg ccttcagctc gtcgcattgg ccggccaacc gggcaaggaa gttcgggtcc
     1981 acaggtctct ccgggcaagc gcggattata ggggacccta taatcgcgcc gtgagccgga
     2041 aatccgcgca cgatgaagat gcaagccccg ggatggacgc aggggctggc gcgagcctgc
     2101 gcatcgacaa gtggctctgg tgcgcgcggt tctaccggag caggggcctt gcccaggagg
     2161 cggtggaggg cggccacgtg caggtgaacg gcgaccgggt caaggcgtcc cggcaggtga
     2221 aggtcggcga cagcctgcga atcgtgcgcg gccaggagcg cttcgacgtg gtggtcaccg
     2281 gcattcccgg gcgtcgcggc ccggcgaccg aagcccgtgg gcactatgcc gagacggcgg
     2341 agagcgaagc agcgcgagcg gtcgcccgcg agttcaaccg cctgaccgcg ccggcgcact
     2401 cggggcgccc ggacaagcgc gagcgccgcg acctgctgaa gcacttcaag aaccgatgac
     2461 cgcctcgctc tcctcgcgct cgacgcggct gttcatcggg ctcacggcgt tcttcgtcgg
     2521 caacgcgatc gtcgccgagt tcgtcggcgt gaagatcttc gcgctggaga cgacgttcgg
     2581 cctgcagccg ttcggctgga acctgttcgg ccagtccggg tcgctcagct tcacggccgg
     2641 cgtcctgatc tggccgatcg tgttcatcat gaccgacgtc atcaacgaat acttcggccg
     2701 ccggggcgtg cggctgatct cgtttgcggc ggcgggcatg atcacgtatg ccttcttctt
     2761 cgcctacgcg gccatgcacc tcgcgcccgc ggcctggtgg gtgaccgtca attccgcgcg
     2821 cggcgtgccg gacatgcagg ccgcctacga cacgatcttc ggccagggac tgtggacgat
     2881 cggcgggtcg ctcgccgcgt tcctggtcgg ccagctggtg gacgtggcga tctttcaccg
     2941 catccgggag tcgaccggcg agcgctggat ctggctgcgc gcgacggcgt ccaccgcggt
     3001 ctcgcaactc gtcgacagct ttctcgtgct ctacgtcgct ttcgtcctcg ggccgcagca
     3061 gtggccggtg tcgctgttcc tcgccgtggg aacggtcaac tactgctaca agatggtcat
     3121 ggccgtactg ctgagcccgc tgatctacgt cgcccgcaac ggcatcgaac gctatctggg
     3181 ccatgccgag gccgcacggc tcaaggcgga ggcggcggcc gagtgaccgg ccagggttgc
     3241 ggaccgctgc tgacgctgcg ggagcacgag aggctgctcg cgggccgcga gcgaggctcg
     3301 gccgtcgtcg agtgctcgct ggacctcggc cgttccaccg acctcgtgaa ggctgggccg
     3361 gacgcctgga cctggcgtgg cgtgcagttt ccctatctgc cgacctgcag ggaacgagcg
     3421 gtttactact gggaggacgg gcagtttcgg ctggccgcgc gcttcggcag gtcgctggtc
     3481 aagctggtgc ccacggagtg gggcgcgccg acgttcgaga tcgacggcat caagatgctg
     3541 ccgacggatg cgtcatcgcc ctacgaggac gcgcgtcgga agatcgacct cgtccgggtg
     3601 cgcggccgcg ccgtgctcga cacctgcggc gggctcgggt actacgcggc ctggtgcctc
     3661 g
//
LOCUS       DAOMFD010000196         3627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_12123_length_3627_cov_37.091545, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000196 DAOMFD010000000
VERSION     DAOMFD010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3627
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12123_length_3627_cov_37.091545"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..350)
                     /locus_tag="PL152_09475"
     CDS             complement(<1..350)
                     /locus_tag="PL152_09475"
                     /inference="COORDINATES: protein motif:HMM:NF013052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein-lysine
                     N-methyltransferase"
                     /protein_id="HQR49556.1"
                     /translation="MRHASRSAVAAESPAFEVRRSGIHGRGVYATRRIPKHTRIVEYL
                     GEVITHEEADARYAAKGQDDGHTFLFVVSKRLVIDAGVAGNDARFINHSCDPNCDTVI
                     EDDRVFIESLRDIEP"
     gene            complement(410..1204)
                     /gene="thyA"
                     /locus_tag="PL152_09480"
     CDS             complement(410..1204)
                     /gene="thyA"
                     /locus_tag="PL152_09480"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HQR49557.1"
                     /translation="MRQYLDLMRHVLERGSRKTDRTGTGTLATFGWQMRFDLADGFPL
                     VTTKKLHVKSIIHELLWFLRGETNVRYLRENGVTIWDEWADEQGELGPVYGVQWRSWR
                     AADGRTIDQVQAAVELLRRDPHSRRILVNAWNVGELDRMALTPCHALYQFYVADGRLS
                     CQLYQRSADIFLGVPFNIASYALLTHMLAQQCDLAPGEFIWTGGDCHLYVNHLEQARL
                     QLSREPLPLPRLEIKRRPESVFEYRYEDFEVAGYQSHPAIKAPIAV"
     gene            complement(1208..1999)
                     /gene="lgt"
                     /locus_tag="PL152_09485"
     CDS             complement(1208..1999)
                     /gene="lgt"
                     /locus_tag="PL152_09485"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009393202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HQR49558.1"
                     /translation="MFVYSNPDPVAISIGPLRVRWYGLMYLVGFLGAWWLARRRAARP
                     GSTWTPVDVDDLIFYCAVGVILGGRLGWMLFYGTERIMTDPLSVLRIWEGGMSFHGGL
                     LGVVVALAMFASRRGRAIVDVFDFTAPLPAIGFAAGRIGNFINGELWGKPTDAPWGFV
                     VDGVARHPSQLYEAFLEGLVLFSVLWWFTSRPRPRWAPSGLFLVCYGAFRFAVEFVRV
                     PDENRGYLLLGWVTMGQILSLPMIVAGAWMLAYAYARRQPSGNYA"
     gene            complement(2095..>2412)
                     /locus_tag="PL152_09490"
     CDS             complement(2095..>2412)
                     /locus_tag="PL152_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HQR49559.1"
                     /translation="FDIVIVDPPAFIKRRRDIPKGQAAYRKLNQLAMQLLPRDGILVS
                     CSCSHHLAQEELVAAIQQSARHLNRFAQVVEVGGHAPDHPIHPAIPETRYLKSLTCRV
                     VIE"
     assembly_gap    2413..2512
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2513..3186)
                     /locus_tag="PL152_09495"
     CDS             complement(<2513..3186)
                     /locus_tag="PL152_09495"
                     /inference="COORDINATES: protein motif:HMM:NF036808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmI/RlmK family 23S rRNA methyltransferase"
                     /protein_id="HQR49560.1"
                     /translation="MTHAPDAAREGQDYPELRLRPREDRRIAAGHLWIFSNEVDIAKT
                     PLTGFTAGSICRVFGDRDRFLGYAYVNPHALICARILSRDGRHPPGKSLFVHRLQVAL
                     ALRERLYDKPFCRLAYGESDGLPGLVLDRYGETVVGQVGTAGMEACKGEIVDAVAKVV
                     APRAFIWKNDSGARELEGLPSYVETVFGDPVEHVWVEENGVRFKVPLASGQKTGWFYD
                     QATNRRA"
     gene            3246..>3627
                     /locus_tag="PL152_09500"
     CDS             3246..>3627
                     /locus_tag="PL152_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HQR49561.1"
                     /translation="MQPSGSGTHRNRLARETSPYLLQHAANPVDWYPWGPEALEKARA
                     EDKPILLSIGYSACHWCHVMAHESFEDAATAETMNRLFVNVKVDREERPDLDRIYQTA
                     HQMLTQRGGGWPLTMFLSPRDQRPF"
BASE COUNT          588 a         1181 c         1217 g          541 t
ORIGIN      
        1 ggctcgatgt cgcgcagcga ctcgatgaag acccggtcgt cctcgatgac cgtgtcgcag
       61 ttcgggtcgc aggagtggtt gatgaagcgc gcgtcgttgc ccgccacccc ggcgtcgatg
      121 accaggcgct tgctcacgac gaacaggaag gtgtggccgt cgtcctggcc cttggccgcg
      181 taacgcgcgt cggcctcttc gtgggtgatg acttcgccca ggtattcgac gatgcgcgtg
      241 tgcttcggga tgcgacgcgt cgcgtacacc ccccgcccgt ggatgccgga gcggcggacc
      301 tcgaacgccg gcgactcggc ggcgaccgcc gagcggctcg cgtggcgcat gtgcgggtgg
      361 tgggacgccc cggcggggtc gctcggggcg cgtgatgcgg caggcttctt catacggcga
      421 tgggggcctt gatggctggg tgcgactggt agccggcgac ctcgaagtcc tcgtagcgat
      481 actcgaagac cgactcgggc cgtcgcttga tctcgagtcg cggcaggggc agcggctcgc
      541 gtgacagctg cagtcgcgcc tgttcgaggt gattcacgta caggtggcag tcccccccgg
      601 tccagatgaa ctcgccgggc gcgaggtcgc actgctgcgc aagcatgtgg gtgagcagcg
      661 cgtaggacgc gatgttgaac gggacgccga ggaagatgtc cgcactgcgc tggtagagct
      721 ggcacgacag ccggccgtcc gcaacgtaga actggtagag cgcgtggcac ggcgtgagcg
      781 ccatgcggtc gagctcgccc acgttccagg cgttcaccag gatgcggcgc gagtgcgggt
      841 cgcgcctcag cagctcgacg gccgcctgga cctggtcgat ggtgcgcccg tcggctgcgc
      901 gccacgatcg ccactggacg ccatagaccg ggccgagttc gccctgctcg tcggcccact
      961 cgtcccagat cgtcacgcca ttctcgcgca ggtagcggac gttggtctcg ccgcgcagga
     1021 accacagcag ctcgtggatg atcgatttca cgtgcagctt cttggtggtg acgagcggga
     1081 acccgtcggc gaggtcgaaa cgcatctgcc agccgaacgt ggcgagcgtc cccgtgcccg
     1141 tgcggtcggt cttgcgcgag ccgcgctcga gcacgtggcg catcaggtcg aggtactggc
     1201 gcatggctca ggcgtagttg ccggaaggct gccgtcgcgc gtaggcgtat gcgagcatcc
     1261 acgcgccggc gacgatcatc ggcaacgaca ggatctgccc catcgtgacc cagcccagca
     1321 gcaggtagcc ccggttctcg tccggcacgc gcacgaactc gactgcgaag cggaacgccc
     1381 cgtaacagac caggaacagc ccggacggcg cccagcgagg cctgggccgg ctcgtgaacc
     1441 accacaggac cgagaacagc acgagcccct cgaggaacgc ctcgtacagc tgcgacggat
     1501 gccgcgccac gccgtcgacg acgaaacccc agggcgcgtc ggtcggcttg ccccagagct
     1561 caccgttgat gaagttgccg atgcgtccag ccgcaaaacc tatggccggg agtggcgcag
     1621 tgaagtcgaa gacgtccacg atcgcgcgtc cgcggcggct cgcgaacatc gccagggcga
     1681 ccaccacgcc gagcagcccg ccgtggaacg acatcccgcc ttcccagatg cgcagcacgc
     1741 tcaacgggtc ggtcatgatc cgttcggtgc cgtagaacag catccagccg agccgcccgc
     1801 cgaggatcac gccgacggcg cagtagaaga tcaggtcgtc gacgtccacc ggcgtccacg
     1861 tcgatcccgg tcgtgcggcc ctccggcgcg ccagccacca tgccccgagg aagcccacga
     1921 ggtacatgag gccgtaccag cgcacgcgca gcggcccgat cgagatcgcg accgggtctg
     1981 gattcgaata gacgaacatg aggcgggtag tttagccgct gcggcgggca cgtgcccggt
     2041 cggatggtgt cgttgcccgc gcgggaccgc acttggcgac ggcagacgcc acggtcactc
     2101 gatcacgacg cggcaggtca gggacttgag ataacgtgtt tccggaatcg cgggatggat
     2161 gggatggtcg ggcgcgtgcc cgccgacctc gacgacctgg gcgaagcgat tcaggtgccg
     2221 cgccgactgc tggatcgccg cgacgagttc ctcctgcgcc aggtggtggg agcaggaaca
     2281 cgacacgagg atgccgtcgc ggggcaggag ctgcatggcg agctgattca gcttgcggta
     2341 ggcggcctgt cccttcggga tgtcgcggcg acgcttgatg aacgcgggcg gatcgacgat
     2401 cacgatgtcg aannnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcgcggcg
     2521 gttcgtcgcc tggtcgtaga accagccggt cttctgcccg gaagccagcg gcaccttgaa
     2581 tcggacgccg ttctcctcga cccagacgtg ctcgacgggg tcaccgaaca cggtctccac
     2641 gtacgacggc aggccttcga gttcgcgcgc gccggagtcg ttcttccaga tgaaggcacg
     2701 gggcgcgacc accttggcga cggcgtccac gatctccccc ttgcaggctt ccatcccggc
     2761 ggtgccgacc tggccaacca cggtctcccc gtagcgatcg agcacgagtc cgggcaggcc
     2821 gtcggactcg ccgtatgcga gccggcagaa cggcttgtcg tagagcctct cgcgcaaggc
     2881 cagcgcgacc tgcagccgat gcacgaacag cgacttaccc ggcggatggc ggccgtcgcg
     2941 gctcaggatg cgggcgcaga tgagcgcgtg cggattgacg taggcgtagc ccaggaagcg
     3001 gtcgcggtcg ccgaagacgc ggcagatcga gccagcggtg aagccagtca acggggtctt
     3061 cgcaatgtcc acctcgttgc tgaagatcca caggtggccg gccgcgatgc ggcggtcttc
     3121 gcgcggcctg agcctgagct ccgggtaatc ctgcccttcg cgcgccgcgt ccggagcgtg
     3181 cgtcatggat cggtcactcg ttccgatata gtgggtgcat tctatccgac ccgccgcgtc
     3241 accccatgca gccatccggc tcaggcaccc accgcaatcg cctcgcccgc gagacgagcc
     3301 cctacctgtt gcagcacgca gccaacccgg tggactggta cccctggggc ccggaggcgc
     3361 tcgaaaaggc gcgtgccgaa gacaagccga tcctgctgtc gatcggctat tccgcgtgcc
     3421 attggtgcca cgtcatggcg cacgagtcgt tcgaggacgc cgcgaccgcc gagaccatga
     3481 accggctgtt cgtgaacgtg aaggtggacc gcgaggagcg ccccgacctc gaccgcatct
     3541 accagaccgc gcaccagatg ctgacccagc gtggcggcgg ctggccgctc acgatgttcc
     3601 tctcgccacg cgaccagcgg ccgttct
//
LOCUS       DAOMFD010000197         3617 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_12182_length_3617_cov_34.249017, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000197 DAOMFD010000000
VERSION     DAOMFD010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3617)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3617
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12182_length_3617_cov_34.249017"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..318)
                     /locus_tag="PL152_09505"
     CDS             complement(<1..318)
                     /locus_tag="PL152_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49562.1"
                     /translation="MHALDRRDLVERFELALEAGDGATGAHCIHELWMRNEPPMNVER
                     MLGLLWRAASESVPEWLPMRYVGWLPLAYEVAARFEREGRGRSNIYLVLLDYSDSRSE
                     PYGV"
     gene            complement(547..>1980)
                     /locus_tag="PL152_09510"
     CDS             complement(547..>1980)
                     /locus_tag="PL152_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HQR49563.1"
                     /translation="ATSLAILVGFALPPLLQLKRVPPARVLRRDLEPPPLRFVAVYGI
                     AIASVIALLVWLVRDLRLVGWVAAGLAGTFAALALAGWLLVKALRPLRRGAGVAWRYG
                     LANISRRGRDSVVQVVAFGLGIMVLLLLALVRNDLLREWRASLPADAPNYFMINIRPA
                     DGAAVREFFSERGLPPTELVPLVRARLAAVNGVPVDKLHFESDRAQGFVEREANLTWS
                     RALRDDNRIVAGRWWSDGDGGDARVSVEKEYAERLGLKLGDTLTYDVAGEPVSARITS
                     LRDVRWDSFQPNFFVVFSPGVLEGVTGTLITSVHVDRSQRPLLVDLVRRFPEVTIIDI
                     DALLTQVREVMDKAALAVQYVFAFTLAAGVMVLLAAVQATRDERHFESAILRTLGASR
                     RVVFAGVAAEFVVLGLLAGVLAAAGASIAGYFLAREIFDLKYSADPAVWIAGVAGGAL
                     LVGVAGVLAARSAVTQPPVATLRQVQG"
     assembly_gap    1981..2080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2081..2487)
                     /locus_tag="PL152_09515"
     CDS             complement(<2081..2487)
                     /locus_tag="PL152_09515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49564.1"
                     /translation="MRSLRFALRNLARDLRSGELVVLLVALTLAVAALTAVSFFTSRV
                     SRAVSQQAGEVLAADLRLESARPIDEAYDHEARRRGLAVARILSMPSVVFEGQESSLI
                     TLRAASDGYPLRGRLKIADHPFGEARPATGIPGR"
     gene            complement(2484..3176)
                     /locus_tag="PL152_09520"
     CDS             complement(2484..3176)
                     /locus_tag="PL152_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HQR49565.1"
                     /translation="MQADRNDTVLQAERLGKQVSSPEGTLAILSDVTLTISRGETVAV
                     MGASGAGKSTLLALLAGLDAPTSGTVHLAGNELTALDEDGRAAVRARHVGFVFQSFHL
                     VPSLTALENVMLPLELAGRADARAAALEVLGRVGLAARVGHYPKQLSGGEQQRVAIAR
                     GFVTRPDVLFADEPTGNLDTHTGERIIDLLFELNRATGTTLVLVTHERGLATRCARVI
                     TLDAGRIVADRA"
     gene            3277..>3617
                     /locus_tag="PL152_09525"
     CDS             3277..>3617
                     /locus_tag="PL152_09525"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HQR49566.1"
                     /translation="MRVEQGWVALLQQRLGTAGYGYRVVNASESGETTGGALARLPRA
                     LGKQRPAVVIVELGGNDGLRGLPVADIRANLTGLVRLSRKSGAKVLLIGMRIPPNYGP
                     AYTTAFHQVFG"
BASE COUNT          545 a         1252 c         1237 g          483 t
ORIGIN      
        1 cacgccgtaa ggctcgctgc ggctgtcgct gtagtcgagg agcacgaggt agatgttcga
       61 tcggccgcgg ccctcgcgct cgaatctcgc cgcgacctcg taggcgagcg gcagccagcc
      121 gacgtaacgc attggcagcc actccgggac ggactcggag gccgcacgcc agagcagccc
      181 cagcatcctc tctacgttca tcggcggctc gttgcgcatc cacagctcat ggatgcagtg
      241 cgcgccggtc gcgccgtcgc ccgcctcgag cgccagctcg aatcgctcga cgaggtcgcg
      301 tcggtcgagc gcatgcaaag gccgcgacgc cggagtcgcg gaccgcggtc gtgatcccgg
      361 gcgtcgaccg cgcgtggtgg gccgcggttt cttacctttg cggtcgctcg gggtcgccac
      421 gcaacctcct cggactgtcc ggccaggccg gaggcaatga tgcgccagtg ctggcggtga
      481 tgcccacacc cgcggcgagc tggctcgcag gtcgcccatc gggcaccgat cgacggccga
      541 tggatgtcag ccctggacct gcctgagcgt cgccaccggc ggctgcgtca cggccgaccg
      601 cgcagcgagc actccggcca cgcccaccag caatgcgccg ccggcaacac cggcgatcca
      661 gaccgccggg tcggcggagt acttgaggtc gaaaatctcg cgcgccagga agtatcccgc
      721 gatcgacgcg cctgcggcgg ccaggacgcc cgccagcagc ccgagcacga cgaactccgc
      781 tgcgacacct gcgaacacga cgcggcggct cgcgcccagc gtgcgcagga tggcgctctc
      841 gaaatgccgt tcgtcgcgcg tcgcctgcac cgccgccagc agcaccatca cgcccgcggc
      901 cagcgtgaag gcgaacacgt actgcaccgc gagcgccgcc ttgtccatca cctcgcgcac
      961 ctgcgtcagg agcgcgtcga tgtcgatgat ggtgacttcg gggaaccggc gcacgaggtc
     1021 cacgagcagc ggccgctgcg accgatcgac gtgcacactc gtaatcagcg tcccggtgac
     1081 accttcgagc acgccgggcg agaatacgac gaagaagttc ggctggaacg agtcccagcg
     1141 cacgtcgcgc aggctggtga tgcgcgcgct gaccggttcg cccgcgacgt cgtaggtgag
     1201 cgtgtcgccg agcttgagcc ccagccgttc ggcgtattcc ttttcgaccg agacgcgggc
     1261 gtcgccaccg tccccgtcgc tccaccagcg gccggccacg atccggttgt cgtcgcgcag
     1321 cgcccgcgac cacgtcaggt tcgcctcgcg ctcgacgaac ccctgcgcac gatccgactc
     1381 gaagtgcagc ttgtcgaccg gcacgccatt caccgcggcg agtcgcgcac ggaccagggg
     1441 cacgagttcc gtcggcggca ggccgcgctc cgagaagaat tcccgtaccg cggcaccatc
     1501 ggccgggcgg atgttgatca tgaagtaatt cggggcgtcc gcgggcaggc tcgcgcgcca
     1561 ttcgcgcagc aggtcgttgc gcaccagcgc gaggagcagc agcaccatga tcccgagccc
     1621 gaaagcgacg acctgcacga cgctgtcgcg gccgcgacgc gagatgttcg ccagtccata
     1681 gcgccacgcc acgcctgcgc cgcgccgcag cggccggagt gccttgacca gcagccaccc
     1741 cgccagagcg agcgcggcga acgtcccggc cagtccggcg gcgacccagc ccacgagtcg
     1801 caggtcccgc accagccaga cgagcagtgc gatcacggag gcgatggcga tcccgtagac
     1861 ggccacgaat cgcagtggcg gcggctcgag gtcgcgtcgc agcacgcgag ccggcggcac
     1921 gcgtttgagc tgcagcagtg gcggcaacgc gaatccgacc aggatcgcga gcgacgttgc
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn cgccccggga ttcccgtcgc
     2101 gggccgtgcc tcgccgaacg gatgatccgc gatcttgagg cgaccgcgca gcgggtaccc
     2161 gtccgatgcc gctcgcagcg tgatgagcga ggattcctgg ccctcgaaca cgacactcgg
     2221 catcgacagg atccgcgcga ccgcgaggcc gcgtcgccgc gcctcgtgat cgtaggcctc
     2281 gtcgatcggc cgcgccgact ccagcctcag gtcagcggcg agaacttcgc ccgcctgctg
     2341 gctcacggcg cgcgagacgc ggctcgtgaa gaagctcacg gcggtcagcg ctgcaacggc
     2401 cagggtgagc gcgacgagca gcacgacgag ctcgccggaa cgcaggtccc gggcgagatt
     2461 gcgcagcgcg aatcgcagcg atctcatgcg cggtccgcga cgatccgccc ggcatcgagc
     2521 gtgatcacgc gcgcgcaacg cgtggcgagc ccgcgctcgt gagtgacgag cacgagcgtc
     2581 gtgccggtcg cccggttgag ttcgaacagc aggtcgatga tgcgctcgcc ggtgtgcgtg
     2641 tcgagattgc cggtcggctc gtccgcgaac agcacgtccg gccgggtcac gaagccgcgg
     2701 gcgatcgcca cgcgctgctg ctcgccgccg gagagctgct tcgggtagtg gccgacgcgc
     2761 gctgcgaggc cgacgcggcc gagtacctcg agtgccgcag cccgtgcgtc ggcgcgaccg
     2821 gccagctcga gcggcagcat cacgttctcg agcgccgtga gcgacggcac gaggtggaag
     2881 gactggaaca cgaaaccgac gtggcgggcg cgcacggcgg cgcggccgtc ctcgtcgagc
     2941 gcggtgagct cgttgcccgc gaggtgcacg gtgccgctcg tcggcgcgtc gaggcccgcg
     3001 agcagggcga gcagcgtgga cttgccggcg cccgatgccc ccatgactgc gacggtctcg
     3061 ccgcgggaga tcgtgagggt cacgtccgac agtatggcga gcgttccctc ggggctgcta
     3121 acctgcttgc ccagccgttc cgcctgcagc accgtgtcgt tgcgatccgc ctgcatccgc
     3181 ctcgccttgt tgtgcctggt cctcgtcgga ccggttgcgc gcgccgatgt tcccgtcgtc
     3241 ctcgtcctgg gcgacagcct cagcgcaggt tacgggatgc gcgtcgaaca gggctgggtt
     3301 gccctgctcc agcagcgcct cggcacggca gggtacggtt accgcgtggt gaacgccagc
     3361 gagagcggcg agacgacggg tggcgcgctg gcccggctgc cgcgcgccct cgggaagcag
     3421 cgaccggcgg tggtgatcgt cgagctgggg ggtaacgacg gcctgcgcgg gctgccggtc
     3481 gcggacatac gcgcgaatct caccgggctg gtgcggctga gccggaagtc gggagcgaag
     3541 gtgctgctca tcggcatgcg catcccgccg aactacgggc ccgcgtacac gaccgcgttt
     3601 caccaggtgt tcggcga
//
LOCUS       DAOMFD010000198         3602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_12261_length_3602_cov_28.351847, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000198 DAOMFD010000000
VERSION     DAOMFD010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3602
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12261_length_3602_cov_28.351847"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..356)
                     /locus_tag="PL152_09530"
     CDS             complement(<1..356)
                     /locus_tag="PL152_09530"
                     /inference="COORDINATES: protein motif:HMM:NF013069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49567.1"
                     /translation="MISDFCIRRPIFASVLSIVVTLAGAAALVGLPIEQYPPIAPPQV
                     TVSASFPGADAQTVAQAVAAPIETQVNGADNMLYMQSTSSSTGQMTLTVYFQIGTDPD
                     TAQVQVQNRVNIALPQL"
     gene            complement(373..1461)
                     /locus_tag="PL152_09535"
     CDS             complement(373..1461)
                     /locus_tag="PL152_09535"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR49568.1"
                     /translation="MNPRAQAVPAAVITVAVADVPYEAEFVGETESSQEVEIRARVEG
                     FLESIDYAEGSIVNQGDVLFQMDRKPFEAALDAAKAQLAAQQARLATASANLKRVGPL
                     AAEDALSQKDLDDAQGSRDAAVAAVEGARASVLQAELNLSYTTIRSPLHGATSFARRQ
                     PGSFIAPGPDSLLTYVSALDPMRVNFSISENEQLRFNKLVGAKKLRVAADGGYTVKVV
                     LADGSVVPAPGRVTFGDASYNKETGTFLVRAEVPNKDAMLRPGQFVRVRLGGATWVGA
                     IELPQRAVMQGPQGNFVWVVDGESKAQVRPVTVGPLTGNRWIIAEGLKAGDRVVVDGG
                     TKLAPGVTVKPTEPSAAAQTAAPAPAQG"
     gene            complement(1889..2362)
                     /locus_tag="PL152_09540"
     CDS             complement(1889..2362)
                     /locus_tag="PL152_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49569.1"
                     /translation="MLDKNTMRQHHLEVTYVLGRLFTEHFIRVYKAFDGDLTAAIVLG
                     TIGQYNYRRYYAEVGRSAPEGFHRLAERGDHEPFARPCNALSISQSTGIPRETVRRKI
                     RKLVAKGWVRAAAPDQLVVTRSPARHFADFDLETLEHFVEAAREILRFVEHRSRR"
     gene            complement(2411..>3602)
                     /gene="mutS"
                     /locus_tag="PL152_09545"
     CDS             complement(2411..>3602)
                     /gene="mutS"
                     /locus_tag="PL152_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HQR49570.1"
                     /translation="NLRFGYNRVQGYYIEVSRSQADQVPADWVRRQTVKNAERYITNE
                     LKTFEDRVLGARDRSLARERELYEGLLDRLIERLPALQATAGALASLDVLANLAERAL
                     ALRFVQPQLSDDSVIDIRGGRHPVVEQSIDGPFVPNDLSLHEGRRMLVVTGPNMGGKS
                     TYMRQSALIVILAGIGSYVPADSATIGAVDRIFTRIGAADDLAGGRSTFMVEMTEAAN
                     ILNNATRDSLVLMDEIGRGTSTYDGLSLAWACARHIGRDIGAYTLFATHYFELTALAD
                     ELPACANVHLDATEHGDSLIFLHAVKEGPANQSFGLQVARLAGVPKSIVADARRYLHE
                     LERRDHAARPAMPQRELELPLPVSPAEEDALQELRNMDPDSLTPRVALEMLFKLKEML
                     GRGR"
BASE COUNT          545 a         1211 c         1228 g          618 t
ORIGIN      
        1 agctgcggca gcgcgatgtt gacgcggttc tgcacctgca cctgcgcggt gtcggggtcg
       61 gtgccgatct ggaagtacac cgtcagcgtc atctgcccgg tcgacgagct cgtcgactgc
      121 atgtagagca tgttgtcggc gccgttcacc tgcgtctcga tcggcgcggc gacggcctgc
      181 gcgaccgtct gcgcgtcggc gcccgggaag ctcgcggaaa cggtcacctg cggcggggcg
      241 atgggcgggt actgctctat cgggagcccg accagcgccg cggcgcccgc gagagtcacg
      301 acgatcgaga ggacgctcgc gaagatcggg cgccggatgc agaaatccga gatcactccg
      361 ggatgctccg tctcaaccct gcgccggtgc gggtgcagca gtctgcgcgg ccgccgacgg
      421 ttcggtcggc ttgaccgtca cgccgggcgc gagcttcgtg ccaccgtcca cgacgacccg
      481 atcgcccgcc ttcagtccct cggcgatgat ccagcggttg ccggtcagcg gcccgacggt
      541 caccgggcgg acctgcgcct tggactcgcc gtcgacgacc cagacgaagt tgccctgagg
      601 tccctgcatg acagcgcgct gcggcagctc gatcgcgccg acccaggtcg ccccgcctag
      661 ccgcacacgg acgaactgtc cgggtcgcag catggcgtcc ttgttcggta cctcggcgcg
      721 caccaggaac gtgcccgttt ccttgttgta ggaggcgtcg ccgaatgtga cgcgcccggg
      781 ggccgggacc acgctcccgt cggccagcac caccttgacc gtgtaccctc cgtcagccgc
      841 aactctgagc ttcttggcgc cgaccagctt gttgaatcgc agttgctcgt tctcggagat
      901 gctgaagttc acgcgcatcg ggtcgagagc cgagacgtaa gtaaggaggc tgtcagggcc
      961 cggcgcgatg aaggagcctg gctgccggcg cgcgaaactc gtcgcgccgt gcaggggcga
     1021 gcggatggtc gtgtagctca ggttgagctc ggcctgcagc acgctcgccc gtgcgccttc
     1081 gacggcggcg accgctgcat cgcgcgatcc ctgggcgtcg tcgaggtctt tctggctcag
     1141 tgcgtcctcg gcggccagcg ggcccacgcg cttgaggttc gcgctcgcgg tcgcgaggcg
     1201 cgcctgctgt gccgcgagtt gcgccttcgc agcgtccagg gcggcttcga acggcttgcg
     1261 atccatctgg aagaggacgt cgccctggtt gacgatgctg ccttccgcgt agtcgatcga
     1321 ctcgaggaat ccctcgactc gagcgcggat ctcgacctcc tgcgagctct cggtctcgcc
     1381 gacgaactct gcctcgtacg gcacgtccgc caccgcgacc gtgatgacgg ccgccgggac
     1441 cgcctgtgcg cgcggattca cgggtccatt gccgcgttcg cagcctgtcg ccaggaacgc
     1501 aagcaaaacg atcgggagcg atccgaggat ggttcttcgc atcaaggggt cacgcgtctg
     1561 aggggggggg cgaaattgca gaacgcggga ttttgctcca tctccgctgc aattgcgtgc
     1621 gcccgcaacg ccccggccgc gccgaccggg tggcgccgcc cgaggcgacg gcgcggccac
     1681 gaggtcgtcc cacggggggg caatttgcca ttccattcag agacttgcag agcccgagca
     1741 acgtcggcgc acgccagtcg tccgcatctt ccggaaccgc cagtgcgcca tgcccgtggt
     1801 acccaaaatg ggcagacgcg ttcgggcaag cactggtgcg gtcgcaaccg acgcatgcgc
     1861 agcgaagacc tgacggtctc cccgccgcct agcgtcggct cctgtgttcc acgaatcgca
     1921 gaatctcgcg cgcagcctcg acgaagtgtt cgagcgtctc cagatcaaag tccgcgaaat
     1981 ggcgcgccgg tgacctcgtc acgaccagct ggtcgggtgc tgccgctctt acccaaccct
     2041 ttgccacgag cttccgaatc ttgcgccgca ccgtctcccg gggaatcccg gtcgactgac
     2101 tgatcgacag tgcgttgcac ggacgcgcga agggctcgtg gtcgcctcgt tccgccaggc
     2161 gatgaaagcc ctccggggcg ctccgaccca cctcggcgta gtaacgtcgg tagttgtact
     2221 gcccgatcgt gccaagcacg atggcggccg tcaggtcacc gtcgaaggct ttgtagacac
     2281 ggatgaagtg ctcggtaaac agtcgcccga gcacgtacgt gacctccagg tgatgctgtc
     2341 gcatcgtgtt cttgtccagc atggccgctc ctcgggaatg ccgtgagtaa caagctcgcc
     2401 gctgccagga tcaacgtccc ctgccgagca tctccttcag cttgaagagc atttcgagcg
     2461 ccactcgcgg ggtgaggctg tcgggatcca tgttgcgcag ctcctgcagt gcgtcttcct
     2521 cggccggact gacgggtagc ggcagttcga gctcgcgctg cggcatggcc ggacgcgccg
     2581 catgatcgcg ccgctcgagc tcgtgcaggt aacggcgcgc gtcggcgacg atggacttcg
     2641 gcacgccggc gagcctcgcc acctgcagac cgaagctctg gttggccggg ccttccttga
     2701 cggcgtgcag gaagatcaac gaatccccgt gctcggtggc atccaggtgc acgttggcgc
     2761 aggcgggcag ctcgtccgcc agcgcggtca gctcgaagta gtgcgtggca aacagggtgt
     2821 aggcgccgat gtcgcggccg atgtgccggg cacaggccca ggcgagcgac aggccgtcgt
     2881 acgtgctggt gccgcggccg atctcgtcca tcagcaccag gctgtcgcgc gtggcgttgt
     2941 tgaggatgtt ggccgcctcg gtcatctcca ccatgaaggt cgagcggccg cccgcgaggt
     3001 cgtcggcggc gccgatgcgg gtgaagatgc gatccaccgc accaatcgtc gccgagtcgg
     3061 cgggcacgta gctgccgatt ccggcgagga tcacgatcag cgccgactgg cgcatgtacg
     3121 tggacttgcc gcccatgttg gggccggtca cgaccagcat gcgtcgtccc tcgtgcagcg
     3181 acaggtcgtt cggcacgaat ggcccgtcga tggactgctc gacgacggga tgacgtccgc
     3241 cgcgaatgtc gatgaccgag tcgtcgctga gctgcggctg cacgaagcgc agcgcgagcg
     3301 cgcgctcggc caggttcgcg agcacgtcca gcgacgccag tgcgccggcc gtggcctgca
     3361 gcgcggggag tcgctcgatc agccggtcga gcagcccctc gtacagttcc cgctcgcggg
     3421 ccagcgagcg gtcgcgcgcg ccgagcacgc ggtcctcgaa ggtcttcaat tcgttggtga
     3481 tgtagcgttc ggcgttcttg accgtctggc ggcgcaccca gtcggccggc acctggtcgg
     3541 cctggctgcg gctcacctcg atgtagtagc cctgcacgcg gttgtagccg aagcgcaggt
     3601 tc
//
LOCUS       DAOMFD010000199         3564 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_12498_length_3564_cov_37.053861, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000199 DAOMFD010000000
VERSION     DAOMFD010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3564)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3564
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12498_length_3564_cov_37.053861"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1568)
                     /locus_tag="PL152_09550"
     CDS             complement(<1..1568)
                     /locus_tag="PL152_09550"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF002730.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HQR49571.1"
                     /translation="MARTPRSQSRLPFDSIYRHGFLRAAVATPRVEVASPGSNVAQTI
                     ALVRRAAADDALLVVFPELGLSAYSNEDLFQQDALQEASLAALGQLVEASRDLAPLLA
                     VGLPLRADDRLFNCGVLVHRGRILGAAPKTYLPNYREFYEKRQFAPAAQALSTSLRLL
                     GQDVPFGNQLLFAARNVPGLVLHLEICEDLWVPLPPSTLAALAGATVIANLSASDITV
                     GKADYRRLLCASQSAKCVSAYVYSAAGPGESTTDLAWDGHALIYENGERLAESQRFPL
                     EPGFVTADLDLDHLRQERARLTSFGDSVQVHAALLAGFRRIEFELDVPRGPVPLRRRV
                     ERFPYVPSDPARRDERCAEVYDIQVEGLMKRLSACGLEKVVIGISGGLDSTQAALVAV
                     RAFDRLGLPRTNILGFTLPGFGTSRHTFDSAHSLMKVLGIAAAEIDIRPSAELMLKDI
                     RHPYVRGKPVYDVTFENVQAGERTSHLFRLANHNGALVLGTGDLSELALGFTTYGVGD
                     HMSHYNVNASVPKTL"
     gene            1784..2137
                     /locus_tag="PL152_09555"
     CDS             1784..2137
                     /locus_tag="PL152_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49572.1"
                     /translation="MSDVFKRAKALFGRGEPEENAPPPVKKPVNPWHAVSIVPGARAC
                     DAAKALRGQRFLSRGAPALPLKNCSIGSCTCRYEHYEDRRKGPRRASEIGVAIDGYDE
                     KERRSSGKRGRRIKD"
     gene            complement(2127..2789)
                     /locus_tag="PL152_09560"
     CDS             complement(2127..2789)
                     /locus_tag="PL152_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR49573.1"
                     /translation="MRVAVVPVTPFVQNCSIVWSEQTGVGAVIDPGGDVDRILATVRE
                     QGVTLEKILLTHAHIDHAGGTAELAQALKLPIEGPHRGDQFWIDGLAEQARMFGVASA
                     GGFVPDRWLVDGDLVRVGDLEFEVRHCPGHTPGHVVFFSRSERLAFVGDVLFAGSIGR
                     TDFPGGDYGTLIRAIRTNLFPLGDDVQFVPGHGPMSTFGEERRSNPFVGDAAVRSGNL
                     SP"
     gene            complement(2791..3402)
                     /locus_tag="PL152_09565"
     CDS             complement(2791..3402)
                     /locus_tag="PL152_09565"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HQR49574.1"
                     /translation="MRKIWNTMLRGLVAILPIGLTLYLIYWLAGMAERVFSGVIKLVI
                     PESAYWPGLGLLTGLVVLFAAGLAVNAYLVRRALRFGDRLFARIPVVKTVYVAIRDFT
                     RFFPSSGQGGDLKRVVLVPFGPGKAIGFVTSESAAALGLGSRDDDKVAVYLPMSYMIG
                     GHTVFLPRELLEPTSLSVEAAMRVALMGGVTVAGKDAGHGESH"
BASE COUNT          575 a         1224 c         1269 g          496 t
ORIGIN      
        1 agcgtcttcg gcaccgacgc gttcacgttg tagtgcgaca tgtggtcgcc gacgccgtag
       61 gtcgtgaagc cgagcgcgag ttcggacagg tcgccggtcc cgagcaccag cgcaccgttg
      121 tggttcgcca ggcggaacag gtgcgaggtg cgctcgccgg cctgcacgtt ctcgaacgtg
      181 acgtcgtaca cgggcttgcc gcgcacgtac ggatggcgga tgtccttcag catcagctcc
      241 gccgaggggc ggatgtcgat ctcggcggca gcgattccga gcaccttcat gagggagtgc
      301 gcgctgtcga acgtgtgccg gctggtgccg aagccgggca gcgtgaatcc gaggatgttc
      361 gtgcggggca gcccgaggcg gtcgaacgcc cgcaccgcca ccagcgcagc ctgcgtcgaa
      421 tcgagcccgc ccgagatgcc gatgacgacc ttctcgaggc cgcaggcgct caggcgcttc
      481 atgagaccct cgacctggat gtcgtagacc tcggcgcatc gctcgtcgcg gcgcgcaggg
      541 tcggacggca cgtacgggaa tcgctcgacg cgacgcctga gcggcaccgg gccgcgcggc
      601 acatccagct cgaactcgat gcggcggaag cctgcgagca gggcggcgtg cacctgcacg
      661 ctgtcgccga agctcgtgag ccgcgcgcgt tcctgcctca ggtggtcgag gtccaggtcg
      721 gcggtgacga accccggctc cagcgggaag cgctgcgact cggcgagccg ctcgccgttc
      781 tcgtagatga gcgcatggcc gtcccaggcg aggtcggtgg tcgactcgcc gggtcccgcc
      841 gcggaataga cgtaggccga gacgcacttg gccgactgcg acgcgcagag cagccggcgg
      901 taatcggcct tgccgaccgt gatgtcgctg gccgagaggt tggcgatcac ggtcgcgccg
      961 gccagcgcgg cgagcgtgct cggcggcagc ggcacccaca ggtcctcgca gatctcgagg
     1021 tgcagcacca ggcccggcac gttgcgggcg gcaaacagca gctggttgcc gaaaggcacg
     1081 tcctgcccga gcaagcgcag gctcgtggac aacgcctgcg ccgccggtgc gaactgtcgc
     1141 ttctcgtaga actcgcggta gttcggcagg taggtcttgg gcgccgcacc gaggatgcgt
     1201 ccgcggtgca cgagcacacc gcagttgaag agccggtcgt cggcgcgcaa cggcaggccc
     1261 acggcgagca ggggcgcgag gtcgcggctg gcctcgacca gttgcccgag cgccgcgagc
     1321 gaggcttcct gcagggcgtc ctgctggaag aggtcctcgt tcgagtacgc ggacagcccg
     1381 agttccggga acacgacgag cagcgcgtcg tccgcggcgg cacggcgcac gagggcgatc
     1441 gtctgggcga cgttgctgcc gggagacgcc acctcgaccc gcggcgtcgc cacggcagcg
     1501 cgcaggaatc cgtggcgata gatggaatcg aagggcagcc gcgactgcga ccggggcgtc
     1561 ctggccatgc gggcctctgg atggggcact ccagccagtt tcggcctcac ggacgggtcc
     1621 ggcaagggcc cccggccacg gcgcggaggt gacttaatcc gcggtaccct acgcgcatcc
     1681 tgggcatggc gtccccgggg tgcgacgtga gtcactctcc acggccgctc cgtagggcta
     1741 gcctagggca gcactgcaac cgacatccag gggcgacatc caggtgagcg acgtattcaa
     1801 gcgtgcgaag gccttgttcg ggcgcggcga gcccgaggag aatgcgccgc cgcccgtgaa
     1861 gaagccggtc aacccctggc acgccgtttc gatcgttccc ggggcacgag cctgcgacgc
     1921 cgcgaaggcg ctgcgcggcc agaggttcct gtcgcgcggg gcgccggctt taccactgaa
     1981 gaactgctcg atcgggagtt gcacctgccg ttacgagcac tacgaggatc gccgcaaggg
     2041 gccgcgccgc gccagcgaga tcggcgtggc gatcgacggc tacgacgaga aggagcggcg
     2101 gtcgtcgggc aagcggggcc ggcgcatcaa ggactgaggt tcccggaccg caccgcggcg
     2161 tcgccgacga agggattgct gcgccgctcc tcaccgaacg tggacatcgg cccgtgcccg
     2221 ggcacgaact gcacgtcgtc tccgagcggg aacaggttgg tccggatcgc gcgtatcagg
     2281 gtgccgtagt cgccaccggg aaaatcggtg cggccgatcg aacccgcgaa cagcacgtcg
     2341 ccgacgaacg ccagccgctc cgaccgcgag aagaacacga cgtgacccgg cgtgtgcccg
     2401 gggcagtgcc tcacctcgaa ctcgaggtcg ccgacccgca cgaggtcgcc gtcgacgagc
     2461 cagcggtccg gcacgaagcc gccggcgctc gcgacgccga acatgcgcgc ctgctccgcg
     2521 aggccgtcga tccagaactg gtcgccgcgg tgcggcccct cgatcggcag cttgagcgcc
     2581 tgcgcgagtt ccgcggttcc gccggcgtga tcgatgtggg cgtgggtgag caggatcttc
     2641 tccagggtca cgccttgctc gcgcacggtc gcgaggatgc ggtccacgtc gccgcccgga
     2701 tcgatgaccg cgccgacgcc ggtctgctcg gaccacacga tcgagcagtt ctgcacgaac
     2761 ggcgtgacgg gcacgacggc cactcgcatg tcagtggctc tcgccgtgcc ccgcgtcctt
     2821 gccggcgacc gtcacgccgc ccatcaatgc cacgcgcatc gcggcttcga cggagagcga
     2881 cgtgggctcg agcaactcgc ggggcaggaa caccgtgtgc ccgccgatca tgtagctcat
     2941 gggcaggtac acggcgacct tgtcgtcgtc gcggctgccg aggccgagcg cggctgccga
     3001 ttccgacgtc acgaagccga tcgccttgcc gggcccgaac ggcaccagca cgacgcgctt
     3061 caggtcgccg ccctggccgg acgacgggaa gaaccgcgtg aagtcccgga tcgcgacgta
     3121 caccgtcttc accacgggta tgcgcgcgaa caggcggtcg ccgaaccgga gcgcgcggcg
     3181 cacgaggtag gcgttcacgg ccagcccggc cgcgaacagc accacgagcc cggtcagcag
     3241 cccgaggccg ggccagtagg ccgactccgg gatcacgagc ttgatcacgc ccgagaagac
     3301 gcgctccgcc atgcccgcga gccagtagat gaggtacagc gtgagcccga tgggcaggat
     3361 cgccaccagg cccctgagca tcgtgttcca gatcttgcgc atggtcttca ctcgacgcgg
     3421 gtggcgcagt atgcagagac cgtgcacgcg ccgccagcgc gatcctttat catcgcagcg
     3481 tctccccgga tcgcgcccga tgtcccagcg aacccgcgct ttcgcctgcc tgctcgtgct
     3541 ggcgtgcggc acccacgccg ctgc
//
LOCUS       DAOMFD010000200         3542 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_12630_length_3542_cov_28.447089, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000200 DAOMFD010000000
VERSION     DAOMFD010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3542)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3542
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12630_length_3542_cov_28.447089"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            45..326
                     /gene="ssb"
                     /locus_tag="PL152_09570"
     CDS             45..326
                     /gene="ssb"
                     /locus_tag="PL152_09570"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQR49575.1"
                     /translation="MYDRLAEIAAEYLRKGSQVYVEGRIRTRKWQDKEGRDRYTTEII
                     ANEMQMLGSRAGGGAGTESRAEPRSPAAERPAAAPAASQGSEFDDDIPF"
     gene            369..1709
                     /gene="miaB"
                     /locus_tag="PL152_09575"
     CDS             369..1709
                     /gene="miaB"
                     /locus_tag="PL152_09575"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HQR49576.1"
                     /translation="MPRVHVRTFGCQMNEYDSARMADVLRECGGYEPTDDPSEADLLL
                     LNTCSVREKAQEKVFSLLGQWRTLKQRRPGVIIGVGGCVASQEGEGITDRAPFVDLVF
                     GPQTLHRLPEMIASVQGDGRAVVDVSFPEIEKFDHLPQPRASGATAFVSVMEGCSKYC
                     TFCVVPYTRGEEVSRPLEQVLDEVRGLAEQGVREVTLLGQNVNAYRGPMAGGGHADLA
                     ALIYFVAEQPRIERIRFTTSHPLEFRDSLIEAYRDVPKLAGYLHLPVQSGSDRVLALM
                     KRGHTTLEYKQKIRRLREARPGLSLSSDFIVGFPGETERDFAATMALIEEVGFDQSFS
                     FIYSRRPGTPAASLPDDVTHEEKQRRLARLQQRIDAQALAISRSMVGSVQRVLVERPS
                     RRDARQLAGRTENNRWVNFDGPPSLIDSFVDVVVTEALPNSLRGRLAAPVRSIA"
     gene            1706..2686
                     /locus_tag="PL152_09580"
     CDS             1706..2686
                     /locus_tag="PL152_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HQR49577.1"
                     /translation="MNPQVEVREIALDEADNARLANFAGPMEQNLRHVEERLGVEVRR
                     RGHQLQVYGAAGTVDNAAAVLRRIYELAAGEVMSPERVHLCVQELGFPAADEGRAPVE
                     DVVVQTQRGGVRGRGANQRQYLDNIRLNDLTFGIGPAGTGKTYLAVASAVEALQSGAV
                     RRIVLVRPAVEAGERLGFLPGDLAQKVDPYLRPMYDALYEMMGFDRVARNVERQVIEV
                     APLAFMRGRSLNDSFIILDEAQNTTVEQMKMFLTRLGFGSRAVVTGDITQIDLPRQQR
                     SGLRHAIEVLRGVEGVAFTFFNSRDVVRHPLVQRIVQAYERATGEPGPDA"
     gene            2683..3192
                     /gene="ybeY"
                     /locus_tag="PL152_09585"
     CDS             2683..3192
                     /gene="ybeY"
                     /locus_tag="PL152_09585"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HQR49578.1"
                     /translation="MSGRPGLTVEAQYAARRPWVPARAALERWAGAARRAVLRGRRRK
                     REDAVVHLCVRVVGHAASRRLNRTYRGKDKATNVLSFPASRDERRFGGSLGDLVICAP
                     VVAAEARGQGKALPAHWAHMVVHGVLHLHGYDHEHPRAGRAMERLEVEILRGFGYQNP
                     YLPVTDPGP"
     gene            3189..>3542
                     /locus_tag="PL152_09590"
     CDS             3189..>3542
                     /locus_tag="PL152_09590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HQR49579.1"
                     /translation="MTEDRPRPAGSWLRRIVESLSGEPQDLDDLGEVLADARDRGLIE
                     ADVLAMLEGVLQVSETQVRDVMVPRSQMVVVQRDETLDRILPIVVESGHSRFPVVGED
                     RDEVAGILLAKDLLRY"
BASE COUNT          536 a         1233 c         1270 g          503 t
ORIGIN      
        1 agaccggcga gcagcgcgag cagaccgagt ggcacaacat cgtcatgtac gaccggctcg
       61 ccgagatcgc cgccgagtac ctgcgcaagg gttcccaggt gtacgtcgag ggccgcatcc
      121 gcacgcgcaa gtggcaggac aaggagggcc gcgaccgcta caccaccgag atcatcgcca
      181 acgagatgca gatgctcggc agccgcgcgg gcggcggggc gggcaccgag tcgcgcgccg
      241 agccccggtc gcccgcagcc gagcggccgg ccgcggcccc ggccgcgtcg cagggctcag
      301 agttcgacga cgacatcccg ttctgaatcc gatctgatcg gttcggccac cccgctggtt
      361 tcgcgaactt gcctcgagta cacgtcagga ccttcggctg ccagatgaac gagtacgact
      421 ccgcccgcat ggcggacgtg cttcgcgagt gcggtggcta tgagccgacc gatgatccgt
      481 ccgaggcgga tctcctcctg ctcaacacct gttccgtgcg cgagaaggcg caggagaagg
      541 tgttctcgct gctcggccag tggcgcacgc tgaagcagcg ccggcccggc gtgatcatcg
      601 gcgtgggcgg ttgcgtggcg agccaggaag gcgagggcat cacggatcgc gcgccgttcg
      661 tcgacctcgt gttcgggccg cagacgctgc accggctgcc ggagatgatc gcgtccgtgc
      721 agggcgacgg gcgggccgtc gtcgacgtga gcttcccgga gatcgagaag ttcgaccacc
      781 tgccgcaacc ccgtgcctcg ggcgcgacgg cgttcgtctc ggtcatggag ggctgcagca
      841 agtactgcac gttctgcgtc gtgccctaca cgcgcggcga ggaagtgagc cgtccgctcg
      901 agcaggtgct cgatgaggtg cgcggcctcg cggagcaggg cgtgcgcgag gtgaccctgc
      961 tcgggcagaa cgtcaacgca taccgcgggc cgatggccgg cggaggccac gcggacctcg
     1021 ccgcgctgat ttatttcgtc gcggagcaac cgcggatcga gcgcatccgc ttcacgacct
     1081 cgcacccgct cgaattccgc gacagcctca tcgaggcgta ccgcgacgtg ccgaagctcg
     1141 cgggctacct ccacctgccg gtgcagagcg ggtccgaccg cgtgctcgcg ctgatgaagc
     1201 gcggtcacac cacgctcgag tacaagcaga agatccgtcg cctccgcgaa gcgcgcccgg
     1261 ggctcagcct gtcgtcggac ttcatcgtcg gctttccggg cgagacggag cgcgatttcg
     1321 cggcgacgat ggcgttgatc gaggaggtcg gcttcgacca gagcttcagc ttcatctaca
     1381 gccgccgccc cggcacgccg gccgcctcgc tgcccgacga cgtcacgcac gaggagaagc
     1441 agcggcgcct cgcgcgcctg cagcagcgca tcgacgcgca ggcgctcgcc atcagtcgct
     1501 cgatggtggg ctccgtgcag cgggtgctgg tcgagcgacc ctcgcgccgg gatgcgaggc
     1561 aactggcagg ccgcaccgag aacaaccgct gggtgaattt cgacggcccg ccgtcgctca
     1621 tcgacagctt cgtggacgtg gtcgtcaccg aggcgctgcc gaactcgctg cgcggccgcc
     1681 tcgccgcgcc ggtgcggagc atcgcgtgaa cccgcaggtc gaggtgcgcg agatcgcgct
     1741 cgacgaggcc gacaacgcgc ggctggctaa tttcgcggga ccgatggagc agaacctgcg
     1801 ccacgtcgag gagcgcctcg gcgtggaggt gcggcgccgc ggccaccagc tgcaggtgta
     1861 cggcgcggcc ggcaccgtgg acaacgcggc ggccgtgctc cggcgcatct acgaactggc
     1921 ggccggcgag gtcatgtcgc cggagcgggt gcacctctgc gtgcaggaac tcgggtttcc
     1981 agccgccgac gagggccgtg ctcccgtcga ggacgtcgtc gtgcagacgc agcgcggcgg
     2041 ggtccgcggg cggggcgcca accagcggca gtacctcgac aacatccgcc tgaacgacct
     2101 caccttcggc atcggcccgg ccggcacggg caagacgtac ctcgcggtcg cgagcgcggt
     2161 cgaggcgctg cagtccggcg cggtgcgacg gatcgtgctc gtgcgacctg cggtcgaagc
     2221 gggcgagcgg ctgggtttcc tgcccggcga cctcgcgcag aaagtcgatc cgtacctgcg
     2281 gcccatgtac gacgcgctct acgagatgat gggcttcgac cgcgtcgcgc ggaacgtcga
     2341 gcgccaggtg atcgaggtcg cgccgctcgc gttcatgcgc ggccggtcgt tgaacgactc
     2401 gttcatcatc ctcgacgagg cgcagaacac caccgtcgag cagatgaaga tgttcctgac
     2461 gcggctcggg ttcggttcgc gcgcggtcgt caccggcgac atcacgcaga tcgacctgcc
     2521 gcggcagcag cgctccgggc tgcgccacgc catcgaggtg ctgcgcggcg tcgagggcgt
     2581 cgccttcacg ttcttcaatt cgcgcgacgt cgtgcgccac ccgctcgtgc agcgcatcgt
     2641 gcaggcctac gagcgcgcga cgggcgagcc ggggcccgac gcgtgagcgg gcggccgggg
     2701 ctcacggtcg aagcacagta cgctgcgcgg cgcccatggg tcccggcgcg ggccgcgctc
     2761 gagcgctggg ccggcgctgc gcgccgggcg gtgctccggg gccgccggcg aaaacgcgag
     2821 gacgcggtcg tgcacctgtg cgtccgggtc gtcggacatg ccgccagccg gcgtctcaat
     2881 cgcacctacc gcggcaagga caaggcgacc aacgtgctgt cgttcccggc ctcgcgcgac
     2941 gaacgccggt tcggcggctc gctcggcgac ctcgtcatct gcgcgccggt cgtcgccgcg
     3001 gaggcgcgcg ggcaggggaa ggcgctgccg gcccactggg cgcacatggt ggtgcacggg
     3061 gtcctgcacc tgcacggtta cgaccacgag catccgcgcg ctggccgcgc gatggagcgc
     3121 ctcgaggtgg aaatcctgcg cggattcggg tatcagaatc cgtaccttcc cgtcaccgac
     3181 ccgggaccat gaccgaagac cgccccaggc ccgcgggcag ctggctgcgc cgcatcgtcg
     3241 aatccctctc cggcgagccc caggacctcg acgacctcgg cgaagtgctg gcggacgcca
     3301 gggaccgcgg cctgatcgaa gccgacgtgc tcgcgatgct cgagggcgtg ctgcaggtct
     3361 ccgagaccca ggtgcgcgac gtcatggtgc cccgctcgca gatggtcgtc gtccagcgcg
     3421 acgagacgct ggaccgcatc ctgccgatcg tcgtcgagtc gggacattcc cgcttcccgg
     3481 tcgtgggcga ggaccgcgac gaggtcgccg gcatcctgct cgccaaggac ctgctgcgct
     3541 ac
//
LOCUS       DAOMFD010000201         3502 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_12876_length_3502_cov_39.674790, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000201 DAOMFD010000000
VERSION     DAOMFD010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12876_length_3502_cov_39.674790"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1192)
                     /gene="eno"
                     /locus_tag="PL152_09595"
     CDS             complement(<1..1192)
                     /gene="eno"
                     /locus_tag="PL152_09595"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011001079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQR49580.1"
                     /translation="MTKIVDVVAREIMDSRGNPTIEADVRLDGGIVGRAAVPSGASTG
                     TREAVELRDGDKARYFGKGVRKAVANVNGEIRSAVLGRDARDQAGLDQRLISLDGTET
                     KSRLGANAILAVSLAAGHAAARAAGQGLWRSLGGPGPKVMPVPMMNIVNGGAHADNSV
                     DIQEFMVLPVGAPSFAEALRYGAEIFHTLKKVLHERGLATSVGDEGGFAPNLPSNEAA
                     LETILEAIERAGFKAGRDVCLGLDVASSELFRDGVYELESEGRKFTPAQFVDYLAGLA
                     DRYPIITIEDGMAEGDWDGWSILTQRLGRRLQIVGDDLFVTNTKILREGIARGIANSI
                     LIKPNQIGTLTETLAAIEMAAQAGYSSVVSHRSGETEDSTIADIAVATKATQIKTGSL
                     CRSDR"
     gene            complement(1189..2022)
                     /gene="kdsA"
                     /locus_tag="PL152_09600"
     CDS             complement(1189..2022)
                     /gene="kdsA"
                     /locus_tag="PL152_09600"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012364209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HQR49581.1"
                     /translation="MRLCGFEVGPDRPLFLIAGPCVIESEALVMEVAGRMREIALELG
                     IPYIFKASFDKANRSSRSSFRGPGLDAGLRILAKVRAELGLPVLTDVHEDTPLAEVAA
                     VVDVLQTPAFLCRQTNFVVNVASQGKPVNVKKGQFLSPWEMANVVEKARSTGNQQILV
                     CERGYSFGYNNLVSDMRSLAIMRATGAPVVFDATHSVQLPGGQGSASGGQREFVPVLA
                     RAAVAAGVSGVFMETHPDPARALSDGPNAWPLDRMRPLLGLLKEIDAAVKSRPYEESC
                     L"
     gene            complement(2019..>3502)
                     /locus_tag="PL152_09605"
     CDS             complement(2019..>3502)
                     /locus_tag="PL152_09605"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HQR49582.1"
                     /translation="HGEVFVTDDGTETDLDLGHYERFVRTTTGRNSNFTTGRIYERVI
                     AKERRGDYLGATVQVIPHITDEIKHCIRQGAGDADVCMVEIGGTVGDIESLPFLEAIR
                     QLGVELGHDNVLYMHLTLIPYIATSGEIKTKPTQHSVKELRSIGIQPDVLLCRCRDPL
                     PPEQRRKIALFTNVEERAVFSAVDADDIYKIPSLLHEQELDAIVCHKLRIEAPPADLH
                     EWQQVVAAKVNPDLTVNIAMVGKYVNLKDAYISLTEALMHAGLRTRTRVKLNFVEATD
                     IEKHGTAVLEGMDAVLVPGGFGERGIEGKIEAVRYARENGVPYLGICLGMQLAIIEYA
                     RHVAGLEGAHSTEFNRENPYPVVALITEWQDQKGTVEARSEQSNLGGTMRLGAQEVRI
                     SHGSLAHSVYGADVIRERHRHRYEFNNNYLQRMMNAGLRFSGFSRDGLVEIVELPHHP
                     FFIASQYHPEFRSTPRDGHPLFTGFIRAARDYRVAQLPAAAGA"
BASE COUNT          504 a         1188 c         1212 g          598 t
ORIGIN      
        1 tgcggtcgga gcggcacagc gatcccgtct tgatctgcgt ggccttggtc gcgaccgcga
       61 tgtcggcgat cgtgctgtcc tcggtctcgc ccgagcgatg cgacacgacc gagctgtagc
      121 ccgcctgcgc ggccatctcg atcgcggcca gggtctcggt gagcgtgccg atctggttgg
      181 gcttgatcag gatcgagttg gcgatgccgc gtgcgatgcc ctcgcgcagg atcttcgtgt
      241 tggtgacgaa caggtcgtcg cccacgatct gcagccggcg tcccaggcgc tgcgtgagga
      301 tcgaccagcc gtcccagtcg ccctccgcca tgccgtcctc gatcgtgatg atcgggtagc
      361 ggtcggcgag gcccgcgagg taatccacga actgcgcggg cgtgaacttg cggccttccg
      421 actcgagctc gtagacgccg tcacggaaca gttcggagct ggcgacgtcg aggcccaggc
      481 acacgtcgcg gccagccttg aagccagccc tttcgatcgc ctcgaggatc gtctccagcg
      541 ctgcctcgtt cgatggcagg ttcggcgcga agccgccttc gtcacccacc gacgtcgcga
      601 ggccccgctc gtgcaggacc ttcttcagcg tgtggaagat ctcagcgccg tagcgcagcg
      661 cctcggcgaa gctcggcgcg ccgaccggca gcaccatgaa ctcctggatg tcgacgctgt
      721 tgtcggcgtg ggccccgccg ttgacgatgt tcatcatcgg caccggcatg accttcggcc
      781 cgggcccgcc gagcgaacgc cacaggccct gccccgcggc gcgtgcggct gcgtgcccgg
      841 cggcgagcga gacggccagg atggcgttcg ctccgaggcg tgacttggtc tccgtcccgt
      901 cgagactgat caaccgctgg tcgagtcccg cctggtcgcg cgcatcgcgc ccgagcacgg
      961 ccgagcggat ctcgccattg acgttcgcca cggccttgcg cacgcccttg ccgaagtagc
     1021 gcgccttgtc gccgtcgcgc agttccactg cctcgcgcgt gccggtcgac gctccggaag
     1081 gcaccgcggc ccggccgacg atgccgccgt ccagccgcac gtccgcctcg atggtgggat
     1141 tgccgcgcga atccatgatc tcgcgggcga cgacgtcgac gatcttggtc ataggcatga
     1201 ttcctcgtag ggccgcgact tcaccgcggc atcgatttcc ttcaacaacc cgagcagcgg
     1261 gcgcatgcga tcgagcggcc acgcattggg accgtccgag agcgccctgg ccggatcggg
     1321 atgcgtttcc atgaacacgc cggacacgcc ggcggcgacc gccgcgcggg ccagcaccgg
     1381 cacgaactcg cgctggccgc cggacgcgct gccctgcccg cccgggagtt gcacggagtg
     1441 cgtggcatcg aacacgaccg gtgccccggt ggcgcgcatg atggcgaggc tgcgcatgtc
     1501 cgacacgagg ttgttgtagc cgaagctgta gccccgttcg cagaccagga tctgctggtt
     1561 gccggtcgag cgcgccttct cgacgacgtt ggccatctcc cagggcgaga ggaactgccc
     1621 cttcttgacg ttgaccggct tgccctgcga ggcgacgttc acgacgaagt tcgtctggcg
     1681 gcagaggaag gccggcgtct gcaggacatc gacgaccgcg gccacttccg ccagcggcgt
     1741 gtcctcgtgc acgtcggtca gcaccggcag gccgagttcc gcgcgcacct tcgccaggat
     1801 tcgcagtccg gcgtccagcc ccggcccgcg gaagctcgag cgcgacgacc ggttggcctt
     1861 gtcgaacgac gccttgaaga tgtacgggat gccgagctcg agcgcgatct cgcgcatgcg
     1921 gcccgcgacc tccatcacca gcgcctcgct ctcgatgacg cagggcccgg cgatcaggaa
     1981 cagcggccgg tcgggaccga cttcgaaccc gcagagcctc atgcgccggc cgcggcgggc
     2041 agctgcgcga cccggtagtc gcgcgccgca cggatgaagc ccgtgaacag cgggtgcccg
     2101 tcacggggcg tggagcggaa ttccgggtgg tactggctcg cgatgaagaa cgggtggtgc
     2161 ggcagctcga cgatctccac cagcccgtcg cgcgagaagc ccgagaagcg caggccggcg
     2221 ttcatcatcc gctgcaggta gttgttgttg aactcgtagc gatgccggtg gcgctcgcgg
     2281 atcacgtcgg cgccgtacac cgagtgcgcg agcgagccgt gggagatgcg cacttcctgc
     2341 gccccgaggc gcatcgtgcc gccgaggttc gactgctcgc tgcgggcctc gacggtgccc
     2401 ttctggtcct gccactcggt gatgagcgcg acgacggggt acgggttctc ccggttgaac
     2461 tcggtgctgt gcgcgccctc gagcccggcg acgtgacgcg cgtactcgat gatcgcgagc
     2521 tgcatgccga ggcagatgcc gaggtacggc acgccgttct cgcgcgcgta gcgcacggcc
     2581 tcgatcttgc cctcgatgcc gcgctcgccg aagccgccgg gcacgaggac cgcgtccatt
     2641 ccctcgagga cggccgtgcc gtgcttctcg atgtcggtcg cctcgacgaa gttgagcttc
     2701 acgcgcgtgc gcgtgcgcag cccggcatgc atcagcgcct cggtgagcga gatgtacgcg
     2761 tccttgaggt tcacgtactt gccgaccatg gcgatgttga ccgtgaggtc cggattgacc
     2821 ttggcggcca cgacctgctg ccactcgtgg aggtccgccg gcggcgcctc gatcctgagc
     2881 ttgtggcaga cgatggcgtc gagttcctgc tcgtgcagca gcgacgggat cttgtagatg
     2941 tcgtcggcgt ccaccgcgga gaacacggcg cgctcctcga cgttggtgaa cagcgcgatc
     3001 ttgcgccgct gctcgggcgg cagcgggtcg cggcagcggc acagcagcac gtcgggctgg
     3061 atgccgatcg atcgcagctc cttgaccgag tgctgggtcg gcttggtctt gatctcgccg
     3121 gaggtcgcga tgtacggaat cagcgtgagg tgcatgtaca gcacgttgtc gtggccgagc
     3181 tcgaccccga gctggcggat ggcctcgagg aacggcagcg actcgatgtc gccgaccgtg
     3241 ccgccgatct cgaccatgca gacgtcggcg tccccggcgc cctggcggat gcagtgcttg
     3301 atctcgtcgg tgatgtgcgg aatcacctgg acggtcgcgc cgaggtagtc gccgcgacgt
     3361 tccttggcga tcacccgctc gtagatgcgc ccggtcgtga aattgctgtt gcggcccgtc
     3421 gtggtgcgga cgaagcgctc atagtgcccg aggtcgaggt cggtctcggt gccgtcgtcg
     3481 gtgacgaaca cctcgccgtg ct
//
LOCUS       DAOMFD010000202         3461 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13155_length_3461_cov_23.271873, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000202 DAOMFD010000000
VERSION     DAOMFD010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13155_length_3461_cov_23.271873"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PL152_09610"
     CDS             <1..377
                     /locus_tag="PL152_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49583.1"
                     /translation="LGDSSGKSKTLNEFKVNAQYAYDQTHVFTGGLFDTWGPRNAAMY
                     APDPVDGSANGSPNSKGYVLEFDWVPWGKLTSVGQPWMNARFGLQYTGYTEFNGGDNN
                     YDGFGRSAGDNNTLYMFIWLAI"
     gene            complement(450..1994)
                     /locus_tag="PL152_09615"
     CDS             complement(450..1994)
                     /locus_tag="PL152_09615"
                     /inference="COORDINATES: protein motif:HMM:NF015213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HQR49584.1"
                     /translation="MLGSAVIVFREVLEAALVIGIVLAATRGLVSSRAWVAGGVIAGT
                     LASLAVAGGAESIASAFEGVGQELLNASILGLAVVMLAWHSVWMTRHGAEIARDMKAV
                     GGEVRSGSRPLHLLALVVGLAVLREGSEVVLFLYGIAAGGADARSLFAGSMLGLAAGV
                     AAGALMYFGLLRIPTHRLFAVTGWLIVLLAAGMAGQAAAFLVQAGVLPGLVEPIWDSS
                     AWLPEPGVVGQVLHALAGYDDRPSATQVLAFLATFTGITVLSKFVNRPRARLRGAAAV
                     PVLLVGIAAAIHSPPTRADHVVYSPLVEAGEKAVEIRGHYDFDSSRDLDGGQAYKVEY
                     EWAPYSRWLTELLVEYEREPGENLDATEIASENIFQLTEQGQYWADFGLLAEFAYNLE
                     RGEPNALELGLLAQKDWGRNEFRVNVLFEQELESGADVETEYRWQYRYRLAEHFEPGL
                     EMYGGAGEWGDFGSFDDHEQQLGPAVFGKFRTADGAVKYEFGVLFGLTSETPDTTVRF
                     MLEYEF"
     gene            complement(1998..2336)
                     /locus_tag="PL152_09620"
     CDS             complement(1998..2336)
                     /locus_tag="PL152_09620"
                     /inference="COORDINATES: protein motif:HMM:NF024864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HQR49585.1"
                     /translation="MILTRRHLAAAAGLALLAAQGALAALPEFTLVIQDHRFSPAELV
                     VPAGQKIRLVVENKDPTPEEFESYTLNREKVVPGNGKIVLYVGPLDAGRYEFFGEFNM
                     ATARGWLIAR"
     gene            2490..3305
                     /locus_tag="PL152_09625"
     CDS             2490..3305
                     /locus_tag="PL152_09625"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HQR49586.1"
                     /translation="MEIPGCRDVGLYGQAWLPDGPSRAVVVASHGLAEHGGRYAGLAS
                     SLTERGLAVYALDHRGHGRSGGPRANIENFDYLVSDLAAFIGRAQRNHPGANVILLGH
                     SMGGAVALACALKYEDALHALVLSAPALAPPEPPSALKRLVVKTLSALRPDAGVLTLP
                     ATAVSRDPQVVGAYVADPLVFHGAIPARTLAELFDAMQRLQQSAHLLRLPVLIQHGAA
                     DRLVPLASVQPVYQQVGLPQRRTLRVYEGLYHEVYNEPERDTVIRDVVNWLGV"
BASE COUNT          575 a         1176 c         1191 g          519 t
ORIGIN      
        1 agctcggcga ctcctcgggc aagagcaaga ccctgaacga gttcaaggtg aatgcccagt
       61 acgcgtacga ccagacccac gtgttcacgg gtggactgtt cgacacctgg ggaccgcgga
      121 acgcggcgat gtacgcgccg gatccggtcg acggcagcgc caacggcagc ccgaacagca
      181 agggctacgt gctcgagttc gactgggtgc cgtggggcaa gctgacgtcg gtcgggcagc
      241 cgtggatgaa tgcacgcttc ggcctgcagt acacgggata cacggagttc aacggcggcg
      301 acaacaatta cgacggcttc gggcgctcgg ccggggacaa caacacgctg tacatgttca
      361 tctggctggc gatctgagcg cggcctgcga ccgcctgaca cgcgggcgac cggcgggaat
      421 cacgtcccgc cggtcgcgcc cggtgcggct cagaattcgt actcgagcat gaagcgcacg
      481 gtcgtgtcgg gcgtttcgct ggtgagcccg aagagcacgc cgaactcgta cttgactgcg
      541 ccgtctgcgg tgcggaactt gccgaacacg gccgggccga gttgctgctc gtggtcgtcg
      601 aacgagccga agtcgcccca ttcgcccgca ccgccgtaca tctcgaggcc gggctcgaag
      661 tgctcggcca ggcgatagcg gtactgccac cggtactcgg tctcgacgtc cgcgccgctc
      721 tcgagttcct gctcgaagag cacgttgacg cggaactcgt tgcggcccca gtccttctgc
      781 gcgagcagcc ccagctcgag ggcgttcggt tcgccgcgct ccaggttgta ggcgaattcg
      841 gcgagcagcc cgaagtccgc ccagtactgc ccctgctcgg tgagctggaa gatgttttcc
      901 gatgcgatct cggtcgcgtc gaggttctca cccggctcgc gctcgtactc gacgagcagt
      961 tccgtgagcc atcgggaata tggcgcccac tcgtattcga ccttgtaggc ctggccaccg
     1021 tccagatcgc gactcgaatc gaaatcgtag tggccgcgga tctcgacggc cttctccccg
     1081 gcttccacca gcggggagta gacgacgtga tcagcccgtg tcggaggaga gtggatcgcg
     1141 gccgcaatgc cgacgagcag cacgggcacg gccgcggcgc cgcgcaaccg cgcccggggt
     1201 cgattcacga acttcgacag caccgtgatg cccgtgaacg tggcgaggaa cgccaggacc
     1261 tgcgtcgcgc tcggtcgatc gtcgtagcca gccagtgcgt gcagcacttg gccgacgacg
     1321 cccggctcgg gcaaccaggc cgagctgtcc cagatgggct cgaccaggcc cgggagcaca
     1381 ccggcctgga cgagaaatgc cgcggcctgt cccgccatcc cggccgcgag gagcacgatc
     1441 aaccagccgg tgacggcgaa caggcgatgc gtggggatgc gcagcaggcc gaagtacatg
     1501 agcgcgcccg cggcaacgcc cgcggccagg cccagcatgg agccggcgaa caggctgcgg
     1561 gcgtcggcgc ctccggccgc gatgccgtac aggaacagca cgacctccga gccctcacgc
     1621 aggacggcga ggcccacgac gagcgcgagc aggtgcagcg gccggctgcc cgagcgcacc
     1681 tcgccgccga ccgccttcat gtcgcgcgcg atctccgcgc cgtgccgggt catccacacg
     1741 ctgtgccagg cgagcatgac caccgcgagg ccgaggatcg aggcgttcag cagttcctgg
     1801 ccgacgccct cgaatgccga cgcgatggac tccgcgccgc cggccacggc cagggacgcg
     1861 agcgtgccag cgatcacgcc tccggccacc catgcgcggc tggagacgag gccacgggtc
     1921 gcagcgagga cgatgccgat gacgagcgcg gcctcgagga cttcacggaa gacgatgacg
     1981 gcggagccga gcatggctca cctcgcgatc agccagccgc gcgcggtggc catgttgaat
     2041 tcgccgaaga actcgtaccg gcccgcgtcc agcggcccga cgtaaagcac gatcttcccg
     2101 ttgccgggga ccaccttctc gcggttcagt gtgtagctct cgaattcctc gggcgtcgga
     2161 tccttgttct cgacgacgag gcggatcttc tggccggcgg gcaccaccag ctccgccggg
     2221 ctgaagcggt ggtcctggat caccagggtg aattcgggga gagcagccaa tgcgccctgg
     2281 gccgcgagga gggcgaggcc cgcggcggct gcgaggtgtc gtcgagtgag gatcaagtgg
     2341 gtcgtcctgg ggcgctggag gtgggagtac tatatacgaa taagaatcat tcgcaactaa
     2401 gcacatccac ctacggtctg gaaatgcggt aaaactcacg cagcggtcca cctccagagc
     2461 aaagggactt gggcaccagg cgtctcgaaa tggaaatccc gggctgtcgc gacgtcggcc
     2521 tgtacggcca ggcgtggctg ccggacgggc cgtcccgtgc ggtggtggtc gcctcccacg
     2581 ggttggccga acacggcggc cgctacgccg gcctggcgtc cagcctcacg gagcgtgggc
     2641 tcgccgttta cgcgctcgat catcgcggcc acggcaggtc gggcgggccg cgcgcgaaca
     2701 tcgagaattt cgactacctg gtgtccgacc ttgcggcgtt catcggacgc gcgcagcgca
     2761 accacccggg tgcgaacgtg atcctgctgg gccacagcat ggggggcgcc gtcgcgctcg
     2821 cctgcgcgct caagtacgag gatgcgctgc acgcactggt gctgtccgca ccggcactcg
     2881 caccgcccga gccgccatcc gcactgaagc ggctggtcgt gaagacgctc tccgcactgc
     2941 ggccagatgc gggtgtcctg acgctgcccg ccacggccgt gagccgggac cctcaggtcg
     3001 tcggcgcgta cgtggccgat ccgctggtgt tccacggcgc gattcctgcg cgcacgctcg
     3061 cggagctgtt cgacgccatg cagaggctgc aacagtccgc gcacctgctg cgcctgccgg
     3121 tcctgatcca gcatggcgcg gcggaccgtc tcgtgccgct ggcttccgtg caacccgtct
     3181 accagcaggt cgggctgcca cagcgccgca cgttgcgggt ctacgaaggc ctctaccacg
     3241 aggtgtacaa cgagcccgag cgcgacaccg tcatccgcga cgtggtgaac tggctcggcg
     3301 tctgaggggc tgccgtcgat gcgcagggcg cggcgtgccg cgccggtttc agccccggtc
     3361 gcggcgagcg gcgagcagcg cgcgcgtctc cttctcgacc ttgcgtgcgc cttcgtcgag
     3421 caggtactca cggaacgcgg ccgctgcagg aggcagccag c
//
LOCUS       DAOMFD010000203         3460 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13159_length_3460_cov_37.654919, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000203 DAOMFD010000000
VERSION     DAOMFD010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13159_length_3460_cov_37.654919"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1313
                     /locus_tag="PL152_09630"
     CDS             <1..1313
                     /locus_tag="PL152_09630"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004015 -
                     adenosylmethionine-8-amino-7-oxononanoate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009102 - biotin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HQR49587.1"
                     /translation="VWHPCTQMKDHEWLPMIPIASGAGVWLYGHDGRRYLDAISSWWV
                     NLFGHANPRINAAVTGQLARLEHVMLAGFTHEPAVRLAERLTEIAPPGLTRCFYADNG
                     SSAIEVALKMSFHYWRNAGHASKRRFVTLANSYHGETLGALAVGHVELYREIYQPLLM
                     DVITVPTPDAYGREAGESEADHARRMFAAMEQALARHADEVCAVIVEPLVQCAGGMRM
                     YDPVYLALLRDACDRHGVHLIADEIAVGFGRTGTMFACEQAGIAPDFLCLSKGLTGGY
                     LPLSVVLTGERVFDAFYDEYASLRAFLHSHSYTGNPLACSAALATLEIFDEDGVIERN
                     RALARHLGERCASLAELPHVAEVRQRGMIVAVELARQGDRAAPFDWRERRGLRVYRHA
                     LEHEALLRPIGNVVYFMPPYVITPDEIDGLVATARAGIEAATCD"
     gene            1304..2038
                     /locus_tag="PL152_09635"
     CDS             1304..2038
                     /locus_tag="PL152_09635"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HQR49588.1"
                     /translation="MRLNRVHCDQSLATGTEIDLPEGAARHVARVLRLRPGATIVAFD
                     GSGHDFTCEITSVAGDAVRVRIGERSAGLRDSPLGITLVQAVSRGERMDWTLQKATEL
                     GVRAIVPVLSARCVVRLDEQQAERRLRHWKAVVAGACEQCGRSTVPEVRAPVELDRYL
                     AQAPREGRRLVLSPDGPASLAGLASTAPRVELLIGPEGGLDGQELDAAGRAGFSPVRL
                     GPRVLRTETAGIVALAVLQALWGDLQ"
     gene            complement(2046..2729)
                     /locus_tag="PL152_09640"
     CDS             complement(2046..2729)
                     /locus_tag="PL152_09640"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR49589.1"
                     /translation="MGAIDLSGKRVLLTQSGEFMGPVLAEVLSELGATVIGDPRALVD
                     PSVPPLVVAAAGHVDVLIVHLALPAPRTAAVDATDDEWRDVFAHLVDPVPRLVRAVLP
                     QMIARRSGKILLMGSAAALRGQKRSSTYSAARGAQLAYVQTVGIEVAPQGVQVNAIAQ
                     NFVDNPTYFPAELQANPAFQERLKRDVPLGRLVSAREDALFAAYLCSDAADCFVGQVF
                     PVCGGWVTR"
     gene            complement(2733..3203)
                     /locus_tag="PL152_09645"
     CDS             complement(2733..3203)
                     /locus_tag="PL152_09645"
                     /inference="COORDINATES: protein motif:HMM:NF013731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HQR49590.1"
                     /translation="MNPVADETYCLLVPLADERLLVPRACVSEVINYQAPTPMEGAPP
                     WYLGTVAWGGRRVPLVSFEAACGRNPPRAGGRTRIVVMQGIAGELPSGHFALPTQGFP
                     QLVRLSPDVIHADDSRTFGERSPVICQVRMVNEYPLIPDFERIERMIAEETRAA"
     gene            complement(3214..>3460)
                     /locus_tag="PL152_09650"
     CDS             complement(3214..>3460)
                     /locus_tag="PL152_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR49591.1"
                     /translation="DVILLDIEMPRMDGYEVASHVRNDPRLADIPIIMITSRAGDKHR
                     SRAIELGVDDYLGKPYQESQLLDAIEPLVLARRRQLE"
BASE COUNT          497 a         1223 c         1243 g          497 t
ORIGIN      
        1 tcgtctggca cccgtgcacc cagatgaagg accacgagtg gctgccgatg atccccatcg
       61 ccagcggggc aggggtctgg ctctacgggc acgacggccg ccgttacctc gacgccatca
      121 gctcgtggtg ggtcaacctg ttcggccacg ccaacccgcg catcaatgcg gccgtgaccg
      181 ggcagctcgc ccgcctcgag cacgtcatgc tggccggctt cacgcacgaa ccggcggtcc
      241 ggctcgccga gcgcctgacg gagatcgccc ctcccggcct cacgcgctgc ttttacgccg
      301 acaatggctc gagcgcgatc gaagtcgcgc tcaagatgag cttccattac tggcgcaacg
      361 ccggccacgc gtccaagcgg cgcttcgtca cgctcgcgaa cagctaccac ggcgagacgc
      421 tgggggcgct ggcggtcggg cacgtcgagc tgtaccgcga gatctaccag ccgctgctca
      481 tggacgtcat cacggtgccc acgccggatg cgtatggcag ggaggctggc gagagcgagg
      541 cggaccatgc gcggcgcatg ttcgcagcga tggagcaggc gctcgcgcgt cacgccgacg
      601 aagtctgtgc cgtgatcgtg gagccgctgg tgcagtgcgc cggcgggatg cgcatgtacg
      661 accccgtgta cctcgcgctg ctgcgcgacg cgtgcgaccg gcacggcgtg cacctgatcg
      721 ccgacgagat cgcggtgggc ttcggccgga ccggcacgat gttcgcgtgc gaacaggccg
      781 gcatcgcgcc ggacttcctg tgcctgtcga agggattgac cggcggttac ctgccgctct
      841 cggtggtgct gaccggcgag cgggtcttcg atgccttcta cgacgaatac gcgagcctgc
      901 gcgcgttcct gcactcgcac agctacaccg gcaatcccct cgcctgctcg gccgcgcttg
      961 cgaccctcga gatcttcgac gaggacggcg tcatcgagcg caatcgcgcg ctggcgcgcc
     1021 acctcggcga gcgctgcgcc tcgctcgcgg agctgccgca cgtcgccgag gtgcgccagc
     1081 gcggcatgat cgtcgccgtg gaactcgcgc gccaaggcga tcgcgcggcg ccattcgact
     1141 ggcgtgagcg gcgcgggctg cgcgtctacc ggcacgcgct cgagcacgag gcgctgctgc
     1201 ggccgatcgg caacgtcgtg tacttcatgc cgccgtacgt gatcacgccc gacgagatcg
     1261 acgggctggt cgcgaccgcg cgcgccggga tcgaggccgc cacgtgcgac tgaaccgcgt
     1321 gcactgtgac cagtccctgg cgaccgggac cgagatcgac ctgcccgagg gtgcggcccg
     1381 tcacgtcgcc cgcgtgctgc gactgcgtcc gggcgcgacc atcgtggcgt tcgacggcag
     1441 tggccacgac ttcacctgcg agatcacgtc ggtcgcgggc gacgcggtgc gggtgcggat
     1501 cggggagcgt tccgcgggcc tgcgcgactc gccgctcggg atcacgctcg tccaggccgt
     1561 ctcgcgtggc gagcgcatgg actggacact gcagaaggcc acggaactcg gggtgcgcgc
     1621 gatcgtcccc gtgctgtcgg cccgctgcgt cgtgcggctc gacgagcagc aggccgagcg
     1681 caggctgcgc cactggaagg ccgtcgtcgc aggcgcctgc gagcaatgcg gccgcagcac
     1741 ggtgcccgag gtgcgcgcgc cggtcgagct cgaccgttac ctggcgcagg cgccgcgcga
     1801 gggccggcgt ctcgtgctga gcccggacgg cccggcgtcg ctcgccggac tcgcgagcac
     1861 ggcaccgcgg gtggaactgc tgatcgggcc ggaagggggt ctcgacgggc aggaactgga
     1921 cgcggctgga cgtgcgggct tcagcccggt gcgattgggc ccacgcgtcc tgcggaccga
     1981 gaccgcgggg atcgtggcgc tcgcggtgct gcaggcgctg tggggcgacc tgcagtagcc
     2041 ttcggtcagc gcgtcaccca gccgccgcag accgggaaca cctgcccgac gaagcagtcc
     2101 gccgcgtcgc tgcacaggta cgcggcgaac aatgcgtcct cgcgcgcgga caccagccgc
     2161 ccgagcggga cgtcgcgctt cagccgctcc tggaacgccg ggttcgcctg cagctccgcg
     2221 ggaaaatacg tcggattgtc gacgaagttc tgcgcgatgg cgttgacctg cacgccttgc
     2281 ggcgcgacct cgatgccgac cgtctgcacg taggcgagct gggcaccgcg cgcggcactg
     2341 taggtcgagg accgcttctg cccgcgcagc gccgccgcgc tgcccatgag caggatcttg
     2401 cccgagcggc gcgcgatcat ctgcgggagc accgcgcgga cgagtcgcgg caccgggtcc
     2461 accaggtgtg cgaacacgtc gcgccattcg tcgtcggtgg cgtcgaccgc cgcggtgcgc
     2521 ggcgcaggca gggcgaggtg gacgatgagc acgtccacgt gcccggcggc cgcgacgacc
     2581 agcggcggta ccgacggatc cacgagggcg cgcgggtcgc cgatgaccgt cgcgccgagc
     2641 tccgacagca cctcggccag cacgggcccc atgaactcgc ccgactgcgt cagcagcacg
     2701 cgcttgccgg aaaggtcgat cgcgcccacg gctcaggcgg cccgcgtttc ttccgcgatc
     2761 atccgctcga tgcgctcgaa gtcgggaatc agcgggtact cgttcaccat gcgcacctgg
     2821 cagatgaccg ggctgcgttc gccgaaggtg cgcgagtcgt cggcgtggat cacgtccgga
     2881 ctcaggcgca cgagctgcgg gaagccctgc gtcggcagcg cgaaatggcc cgacggcagt
     2941 tccccggcga tgccctgcat caccacgatg cgggtccggc cgccggctcg cggcgggttg
     3001 cgcccgcagg cggcctcgaa cgacacgagc ggcacgcgcc ggccgcccca ggcgaccgtg
     3061 cccaggtacc acggcggcgc gccttccatc ggcgtcggcg cctggtagtt gatgacctcg
     3121 gagacgcacg cgcgcggcac gagcagccgc tcgtcagcga gcggcaccag caggcagtag
     3181 gtctcgtcgg ccacgggatt catcggcacc tcgtcactcg agctggcgcc tgcgcgccag
     3241 caccagcggc tcgatcgcgt cgagcaactg gctctcctgg tagggcttgc cgaggtaatc
     3301 gtccacgccg agctcgatcg cgcgcgaccg gtgtttgtcg ccggcccgcg aggtgatcat
     3361 gatgatcggg atgtccgcga gccgcgggtc gttgcgcacg tgcgaggcga cctcgtagcc
     3421 gtccatgcgc ggcatctcga tgtcgagcag gatcacgtcc
//
LOCUS       DAOMFD010000204         3440 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13307_length_3440_cov_34.816248, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000204 DAOMFD010000000
VERSION     DAOMFD010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3440)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3440
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13307_length_3440_cov_34.816248"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="PL152_09655"
     CDS             <1..377
                     /locus_tag="PL152_09655"
                     /inference="COORDINATES: protein motif:HMM:NF025109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4162 domain-containing protein"
                     /protein_id="HQR49592.1"
                     /translation="HYLEEAENLCRNIAIIDEGRIIEHDRMATVLRKLHNEVFVLNLR
                     QPAAGPVELEDYDCMVTADGSLEVEVSKEQNLNDLFAALSARGIEVVSMRNKANRLEE
                     LFMRLVEGRDASTGQPRPAAGP"
     gene            374..1192
                     /locus_tag="PL152_09660"
     CDS             374..1192
                     /locus_tag="PL152_09660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020082510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR49593.1"
                     /translation="MSTTTHPIAGHAAAATTGGRRWVGFRTIVIREFARIIRIWGQTL
                     VPPAITATLYFLIFGSLVGRRIGEMGGFNYSQYIAPGLIMMSVITNSYGNVVSSFFGA
                     KFGRHIEELLVSPLPNWLIVLGYVCGGLLRGLLVGVVVTLISLFFAHLHVEHVLLVCA
                     AVLLTSTVFALCGLINAVFAKNFDQITFIPTFILTPLTYLGGVFYSIAMLPEWAQHAS
                     RANPILYMVNAFRFGFLGVSDVDVGLAFAIMVLSVAALFGASVWLMNRGTGIRE"
     gene            1196..1885
                     /locus_tag="PL152_09665"
     CDS             1196..1885
                     /locus_tag="PL152_09665"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HQR49594.1"
                     /translation="MCGRFAFYSPHEAVTRLFGVEGAPDVEPRYNIAPTQFIATVRPA
                     DGGGRELAMLYWGLVPSWAKEKSIGARMINARSETLAEKPSFRAAYRRRRCLVLADGW
                     YEWQRSGAVKQPYYIGFASGEPFGMAGLWESWHDPASGEPLESCCIVTAPAAPELEFV
                     HDRMPVIIPAAAHQAWLSGGAEVTDGLDALLAPWSRGGLEARPVSRRVNDARNEGADL
                     VTPLGSPGPGA"
     gene            1911..2591
                     /locus_tag="PL152_09670"
     CDS             1911..2591
                     /locus_tag="PL152_09670"
                     /inference="COORDINATES: protein motif:HMM:NF036807.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HQR49595.1"
                     /translation="MAQYDGKIFGIGFYKTGTTTLFEALRMLGYDAVNGDTPGSYPGA
                     DDGATLIRRIDAGDYRLPTFEMFDAFTDNPYFRIWREIYAMFPDARYILTVRDEGPWI
                     ASCTRFFHNRRVRPMRLWMFGPHADPSRNEESRQAWLDAYRQHNRAVREFFATRPHQF
                     LELDPTAGPAWESLCAFLGAPVPSLAWPHANPSRRNLPWRGLWRRLRRALKLEADLPP
                     DDASGDSA"
     gene            2588..3430
                     /locus_tag="PL152_09675"
     CDS             2588..3430
                     /locus_tag="PL152_09675"
                     /inference="COORDINATES: protein motif:HMM:NF018669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase family 2 domain-containing
                     protein"
                     /protein_id="HQR49596.1"
                     /translation="MSGRCDLVAYAHVMKTGGQTMSDILRQSFPGRHCDIQANGVATA
                     DDLRFARRFYPGLRSVAGHGVVPLGNFAEACDSIRYFMFLRDPVERCVSHYQYRRNKD
                     ERSDFEPWLARYANYQSRFLCRAHGMGEAWPEVADGDRAIEVLERHVGFVGLQERFDE
                     SLVLLARWVGDPDFDVAYRSRNVASSSAIRRQLLGDPRTVEMIRAHHVEDEKVYRHVR
                     DHLYPRQVAQYGATLADDVAALKARLPAPQRRVVARSLAAVRRGVFYKPFARTLRAPR
                     RRRR"
BASE COUNT          509 a         1212 c         1161 g          558 t
ORIGIN      
        1 cgcactacct cgaggaagcc gagaacctct gccgcaacat cgcgatcatc gacgaagggc
       61 gcatcatcga gcacgaccgc atggccacgg tgctgcgcaa gctgcacaac gaggtatttg
      121 tcctcaatct gcggcagccg gcggctggcc cggtggagct tgaggactat gactgcatgg
      181 tgaccgccga tggctccctc gaggtcgagg tgagcaagga acagaacctg aacgacctgt
      241 tcgcggcgtt gtcggcgcga ggcatcgagg tcgtcagcat gcgcaacaag gccaatcgcc
      301 tcgaggaact gttcatgcgc ctcgtggagg gccgcgatgc gtcgaccggg cagccgagac
      361 cggcggcggg accatgagta cgacgacaca cccgatcgcc ggccatgctg cggccgccac
      421 tacgggcggg cgccgctggg tcgggtttcg caccatcgtg atccgcgaat ttgcccgcat
      481 catccgcatc tggggccaga cgctggtgcc cccggcgatc accgcgacgc tttacttcct
      541 gatcttcggc agcctggtcg gccgccgcat cggcgagatg ggcgggttca attactcgca
      601 gtacatcgcg cccggcctga tcatgatgtc ggtcatcacc aacagctacg gcaacgtggt
      661 gtcctcgttc ttcggcgcga agttcggccg gcacatcgag gaactgctcg tctcccctct
      721 gccgaactgg ctgatcgtgc tcggctacgt ctgcggcggg ctgctgcgtg gcctgctggt
      781 cggcgtcgtc gtgacgctga tctcgctgtt cttcgcgcac ctgcacgtcg agcacgtgct
      841 gctcgtgtgc gcggcggtcc tgctcacctc gaccgtgttc gcgctgtgcg ggctgatcaa
      901 cgccgtgttc gccaagaact tcgaccagat cacgttcata ccgacgttca tcctgacgcc
      961 gctcacctac ctcggcggcg tgttctactc gatcgcgatg ctgccggagt gggcgcagca
     1021 cgcctcgcgc gccaacccga tcctgtacat ggtgaacgcg ttccgcttcg gcttcctcgg
     1081 cgtgtcggac gtggacgtgg gactcgcgtt cgcgatcatg gtcctgtcgg tcgcggcgct
     1141 gttcggcgcg agcgtgtggc tgatgaaccg gggcaccgga atccgcgagt aggccatgtg
     1201 cggccgcttc gcgttctatt cgccccatga agccgtgacg cggctgttcg gcgtcgaggg
     1261 cgcgccggac gtcgagcccc gctacaacat cgcgccgacg cagttcatcg cgaccgtgcg
     1321 ccccgccgac ggcggcgggc gcgaactcgc catgctgtac tggggcctcg tgccgtcgtg
     1381 ggccaaggag aaatcgatcg gcgcgcgcat gatcaacgcc cgctccgaga ccctggccga
     1441 aaaaccctcg tttcgcgcgg cataccggcg ccgtcgctgc ctcgtgctcg ccgacggctg
     1501 gtacgaatgg cagcgcagcg gcgcggtgaa gcagccctac tacatcggct tcgcgagcgg
     1561 cgagcctttc ggcatggccg gcctctggga gtcatggcac gaccctgcga gtggcgagcc
     1621 gctcgagtcg tgctgcatcg tgaccgcgcc ggcggccccc gagctcgagt tcgtgcacga
     1681 ccgcatgccg gtcatcattc cagcggcggc gcaccaggcg tggctcagcg gcggcgcaga
     1741 ggtgaccgac ggactcgacg cgctgctcgc gccttggtcg cgcggcgggc tcgaagcgcg
     1801 gccggtcagc cgtcgcgtga acgacgcgcg caacgagggc gccgatctcg tcacgccgct
     1861 tggcagcccc ggccccggcg catagagtcg aaccggcgca gggaccggca atggcgcaat
     1921 acgacggcaa gatcttcggc atcggcttct acaagaccgg cacgaccacg ctcttcgagg
     1981 ccctgcggat gctgggctac gacgccgtga acggcgacac gcccggcagc tatcccgggg
     2041 ccgacgacgg ggcgaccctg atccgccgca tcgacgccgg cgactaccgc ctgcccacct
     2101 tcgagatgtt cgacgcgttc accgacaacc cgtatttccg catctggcgc gagatctacg
     2161 cgatgtttcc cgacgcgcgc tacatcctga ccgtgcgcga cgaagggccg tggatcgcga
     2221 gctgcacccg cttcttccac aaccgccgcg tccgcccgat gcgactgtgg atgttcgggc
     2281 cgcacgcgga tccatcgcgc aacgaggaga gccggcaggc ctggctcgat gcctatcgcc
     2341 agcacaaccg cgccgtgcgc gagttctttg cgacccgccc gcaccagttc ctcgagctcg
     2401 acccgacggc gggtcccgcg tgggagtcgc tgtgcgcgtt cctcggcgcg cccgtcccgt
     2461 cactggcgtg gccgcacgcg aacccgagcc gccgcaacct gccgtggcgc gggctctggc
     2521 ggcgcctgcg gcgcgcgctc aagctcgagg cggacttgcc gccggacgac gccagtgggg
     2581 actcggcatg agcggccgct gcgacctcgt cgcctacgcg cacgtcatga agaccggcgg
     2641 acagacgatg agcgacatcc tgcgccagtc gtttccggga cggcactgcg acatccaggc
     2701 caacggcgtc gcgaccgccg acgacctgcg gttcgcgcgc cggttctatc cagggctgcg
     2761 cagcgtggct ggccacgggg tcgtgccgct cggcaatttc gccgaggcct gcgactcgat
     2821 ccgctacttc atgttcctgc gcgacccggt cgagcgctgc gtgtcgcact accagtaccg
     2881 gcgcaacaag gacgagcgct cggacttcga gccgtggctc gcgcgctacg ccaactacca
     2941 gtcgcgcttc ctctgccgcg cgcacggcat gggcgaggcc tggccggagg tggccgacgg
     3001 tgaccgcgcc atcgaggtgc tcgagcgcca cgtcggcttc gtcgggctgc aggagcggtt
     3061 cgacgagtcc ctcgtgctgc tcgcgcgctg ggtcggcgat ccggacttcg acgtcgccta
     3121 ccggtcgcgc aacgtggcgt cctcgagcgc gatccgccgt cagctgctcg gtgatccgcg
     3181 caccgtggag atgatccgcg cgcatcacgt cgaggacgag aaggtctacc gccacgtgcg
     3241 cgaccacctc tacccgcggc aagtggcgca gtacggggcg acgctcgccg acgacgtggc
     3301 ggcgctcaag gcccggctgc cggcgccgca gcgcagggtc gtcgcgcgct cgctggccgc
     3361 ggtgcggcgc ggcgtgttct acaagccgtt cgcgcgcacg ctgcgcgcgc cgcgcaggcg
     3421 tcgacgatga gcgggccagt
//
LOCUS       DAOMFD010000205         3433 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13348_length_3433_cov_33.413262, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000205 DAOMFD010000000
VERSION     DAOMFD010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13348_length_3433_cov_33.413262"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1296)
                     /gene="hemG"
                     /locus_tag="PL152_09680"
     CDS             complement(<1..1296)
                     /gene="hemG"
                     /locus_tag="PL152_09680"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004729 - oxygen-dependent
                     protoporphyrinogen oxidase activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HQR49597.1"
                     /translation="MRIGIVGAGLSGLATAFYLQRLLPQAELAIFESDAQGGGALCTV
                     DVQGFRFEAGGNGFLTNKPDCLQLVNDAGASDLLLPSSDLARKRYIYTNGLYRMPESA
                     QLFATSKLMTLPQKLRMLGEFFVPPRRDGADESLREFGNRRLGPGFTSVFLDALSAGI
                     YGSTPDRISVKAAFPLIVALERDHGGLFRGMLAKRKKEAGPGGVLTSTKGGISTLIRH
                     LESAIRAEWHYGEPVRAVERRGNAYEIHSSGGVTAVDRVVICATSFAAAEMLRPLDAD
                     ISRRLADIQYSPIAVVGFGYRSLKDPLDGFGLLTTTSSKQPILGVLWDSSIFPDRAPA
                     GAKSIRAMIGGQRNPELVDQDDAGLVATARRGIKATMGLDQDPDVTFVKRWDRGIPSY
                     APGHVASIDALFARVGQVPGLYLNCNAYRGIAMNDCARNS"
     gene            1470..2075
                     /locus_tag="PL152_09685"
     CDS             1470..2075
                     /locus_tag="PL152_09685"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HQR49598.1"
                     /translation="MNPVPPVTRALLIANIVIFGLQAVTGDLLVIPFALWPAATAQYP
                     NAPSFEIWQLLTYGFLHGSLTHLFFNMFALWMFGGEVERLLGRAHFLEYYLVCVIGAA
                     VAQLVVMGNMNMPPMPTVGASGGVFGLLLAFGMAWPHRRIMLLFPPIPMPAWLFVTLY
                     GALELYLGVTGSAVGVAHFAHLGGMAAGYALLTYWRSRARR"
     gene            complement(2072..3094)
                     /locus_tag="PL152_09690"
     CDS             complement(2072..3094)
                     /locus_tag="PL152_09690"
                     /inference="COORDINATES: protein motif:HMM:NF002558.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HQR49599.1"
                     /translation="MRPIFQADLVNGPFGDPGLLVDLKFQRRALLFDLGDLGALPTRK
                     MLRVTDVFVSHAHMDHFAGFDRLLRVCLGRDTGVHLYGPPGFIEQVGHKLSAYTWNLV
                     DNYESEFGILVSEIAPEGRVDHARFGSRDRFRRKDLEPTRADAGLLLDDPFFRVRCRF
                     LDHGIPSLAFRFEEGMHINVWKSRLEELGLPTGPWLTELRRLVRENAPAETPIVVRWR
                     DRGGARERSYTVGELRARVLELVPGQTVCYVTDVAWTDANARGIVEFASGADTLYIEA
                     VFLDADGDHAARKLHLTARQAGELARAAGVRVAIPFHFSPRYIEREQDLRREFEDGWR
                     GAASPG"
     gene            3168..>3433
                     /locus_tag="PL152_09695"
     CDS             3168..>3433
                     /locus_tag="PL152_09695"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HQR49600.1"
                     /translation="MASKRIRVSKTAAEDAIRTLLRWAGEDPSREGLRDTPGRVVEAY
                     RDWFSGYAVDPRAYLRRTFEEMAGYDEMVVLRDIEFESHCEHHMA"
BASE COUNT          525 a         1202 c         1165 g          541 t
ORIGIN      
        1 gctgttgcgt gcgcagtcgt tcattgcgat gccgcgatac gcattgcagt tgagatacag
       61 gccgggcacc tggccgaccc gcgcgaacaa cgcgtcgatg ctcgcgacgt gccccggcgc
      121 atagctcggg atgccgcggt cccagcgctt cacgaaggtc acgtcgggat cctggtcgag
      181 gcccatcgtc gccttgatgc cgcgccgcgc ggtcgcgacg agcccggcgt cgtcctggtc
      241 gacgagttcc ggattgcgct gcccgccgat catggcgcgg atgctcttgg cgccggccgg
      301 ggcgcgatcc gggaagatgc tgctgtccca cagcacgccg aggatcggtt gcttcgacga
      361 ggtcgtggtg agcaacccga acccgtcgag cgggtccttg agcgagcgat aaccgaatcc
      421 cacgaccgcg atcggcgaat actgtatgtc cgcgaggcgg cgcgagatgt cggcatcgag
      481 cggccgcagc atctccgcgg cggcaaacga cgtggcgcag atcacgacgc ggtcaaccgc
      541 agtcacgccg ccggatgaat ggatctcgta agcgttgcct cgacgctcca ccgcgcgcac
      601 gggttcgccg tagtgccact cggcccggat ggcgctctcg aggtggcgga tcagcgtgct
      661 gatgccaccc ttggtgctgg tgagcacgcc ccccggcccg gcctccttct tgcgcttggc
      721 gagcatgccc cggaacaggc ccccgtggtc gcgctcgagc gccacgatca gcgggaatgc
      781 agccttgacc gagattcggt cgggcgtcga accatagatg cccgccgaca gcgcatccag
      841 gaacacgctg gtgaagcccg ggccgaggcg ccggttgccg aactcccgca gcgactcgtc
      901 cgcgccgtcg cggcgcggcg gcacgaagaa ctcgcccagc atcctgagct tctgcggcag
      961 cgtcatcagc ttcgacgtgg cgaagagctg cgcggactcc ggcatgcgat acaggccgtt
     1021 ggtgtagatg tagcgcttgc gcgcgaggtc ggaactcggc agcagcaggt ccgacgcgcc
     1081 ggcgtcgttc acgagctgca ggcagtcggg cttgttggtc aggaagccgt tgccgcccgc
     1141 ctcgaagcgg aatccctgca cgtcgacggt gcatagtgcc ccgccaccct gcgcatcgga
     1201 ctcgaagatg gcgagctccg cctgcggcag cagccgctgc aggtaaaagg ccgtcgccag
     1261 cccgctcaat cccgctccaa cgatgccgat gcgcatgatg tgtcctttgc cttcgatcca
     1321 ccccgccccg ggggaccgca ttgtcccgcc cgacgccggg cgccggcagt ccgtgtagac
     1381 cgaggcaagc ttacggaatg ccggaggaac tccgccacgc ctgggcccgc cggcccgccg
     1441 ccggcgaatt cggctaggat tgcgcggcca tgaatcccgt cccgccggtc acccgcgccc
     1501 tgctcatcgc caacatcgtc atcttcggcc tgcaggccgt gaccggcgac ctgctggtca
     1561 tcccgttcgc gctgtggccg gcggcgaccg cgcagtaccc gaacgcgccg tcgttcgaga
     1621 tctggcagct gctcacctac ggcttcctgc acggcagcct gacgcacctg ttcttcaaca
     1681 tgttcgcgct gtggatgttc ggcggcgagg tggaacggct gctcgggcgc gcgcatttcc
     1741 tcgagtacta cctggtgtgc gtgatcggcg cggccgtcgc acagctcgtc gtcatgggaa
     1801 acatgaacat gccgccgatg ccgaccgtgg gagcgtcggg cggcgtgttc gggctgctgc
     1861 tcgcgttcgg catggcgtgg ccgcaccggc gcatcatgct gctgttcccg ccgatcccga
     1921 tgccggcgtg gctgttcgtc acgctctacg gcgcgctcga actctacctc ggtgtgacgg
     1981 gatccgccgt cggcgtcgcg cacttcgcgc acctcggcgg catggccgcg ggctacgcgc
     2041 tgctcaccta ctggagaagc cgggcccgcc gctagcccgg gctcgcggca ccacgccagc
     2101 cgtcctcgaa ctcgcgccgc aggtcctgct cgcgctcgat gtagcgcggc gagaagtgga
     2161 acgggatcgc cacgcgcacc cccgcagcgc gcgccagttc tccggcctgc cgcgccgtca
     2221 ggtgcagctt gcgggcggcg tgatcgccgt ccgcgtccag gaagaccgcc tcgatgtaca
     2281 gcgtgtccgc gccggacgcg aattcgacga tgccccgcgc attggcgtcg gtccaggcga
     2341 cgtcggtcac gtagcagacc gtctgcccgg gcaccagttc gagcaccctg gcgcgcaact
     2401 cgcccaccgt gtagctgcgt tcccgcgcgc caccgcggtc ccgccagcgc acgacgatcg
     2461 gcgtctcggc gggcgcgttc tcgcgcacca gcctgcgcag ttcggtgagc cacgggcccg
     2521 tcgggaggcc gagttcctcg aggcggctct tccagacgtt gatgtgcatg ccctcctcga
     2581 accggaacgc gaggctcgga atcccgtggt cgaggaaccg gcagcgcacc cggaagaacg
     2641 ggtcgtcgag cagcagcccc gcatcggccc gggtcggctc gaggtccttg cggcggaatc
     2701 ggtcgcggct gccgaaccgt gcatggtcga cccgaccctc gggcgcgatc tcgctcacca
     2761 ggatcccgaa ctccgactcg tagttgtcca ccaggttcca ggtgtaggcg gacagcttgt
     2821 gcccgacctg ctcgatgaag cccggcggcc cgtacaggtg cacgcccgtg tcgcggccga
     2881 ggcagacgcg cagcagccgg tcgaaaccgg cgaagtggtc catgtgtgcg tgcgagacga
     2941 acacgtcggt cacgcgcagc atcttgcgcg tcggcagcgc cccgaggtcc ccgaggtcga
     3001 acagcagggc acggcgctgg aatttgaggt ctacgagcag gcccgggtcg ccgaaaggcc
     3061 cgttgacgag gtcggcctgg aagatgggac gcatgctgga ccgtgcgcaa ccgccgccgg
     3121 gcagttagga ttcattctcg tctcgagacc agcccgggga caagcctatg gcaagcaagc
     3181 gcatccgtgt cagcaagacc gcagccgaag acgccattcg cacgctgctg cgctgggccg
     3241 gcgaggaccc ctcgcgcgaa gggctgcggg acacgccggg ccgggtcgtc gaagcctacc
     3301 gggactggtt ctccggctat gccgtggatc cccgcgcgta cctgcggcgc acattcgagg
     3361 aaatggccgg ctacgacgag atggtcgtgc tgcgcgacat cgagttcgag tcgcactgcg
     3421 agcaccacat ggc
//
LOCUS       DAOMFD010000206         3428 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13376_length_3428_cov_34.290839, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000206 DAOMFD010000000
VERSION     DAOMFD010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3428)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3428
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13376_length_3428_cov_34.290839"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="PL152_09700"
     CDS             <1..552
                     /locus_tag="PL152_09700"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR49601.1"
                     /translation="FVEKGVQGTAIEDICTASGASVGSLYHLFDGKNSVAAAVYLDAL
                     EDFQAAVAGSVAADMGAREGVLAIVAAHIRWVEENAARARFLQQARHGEVIAARAADI
                     AEANRRFGRVLAAWMAPQVAAGRLRALPLDLFIALLLGPAQEYVRGRMSGRDSTASDD
                     AIHELGAAAWRSVGRDIPESVSR"
     gene            560..1489
                     /locus_tag="PL152_09705"
     CDS             560..1489
                     /locus_tag="PL152_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49602.1"
                     /translation="MNALHRTYTSAPSALAYMLQAFRPSPGWRSERGCPDMRLTWRNY
                     RVDPAAAAQLAAICGLDGASASTRGAILFPQVTGFRLIMAMLTHASWPLPIWNALQVR
                     NRITLHGPLRADEQYALEVRPSGWRVLAKGLEIDLHTILMHEGQLRWESVVTFYYRGR
                     YGDATESGDSRSAPANSPAVDMENPSAEIVRWKVGDGHRRAFAKLTGDYNGLHQWDAY
                     ARRMGFPAAFPHPQRVVGQALVHLPEADHAPLTLDLWIKGPVFFGCGLLLRHWPATGG
                     AARTFAVWLQGESRPALVGTLGGPTPAQVHGAG"
     gene            1583..2488
                     /locus_tag="PL152_09710"
     CDS             1583..2488
                     /locus_tag="PL152_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HQR49603.1"
                     /translation="MATKRPSLGRGLEALLGKPATSHEPDGSAPAAPRDEELARIPVD
                     LLQRGRYQPRLDMRPESLQELADSIRAQGVVQPIVIRPLAVQRAGEEQRYEIIAGERR
                     WRAAQMAGLHDVPAVIRRVPDEAAVAMSLIENIQRENLNPLEEARALDRLIREFEMTH
                     QTAADAVGRSRAAVSNLLRLLELAEEVKSLVEQRQLEMGHARALLGLTAPRQQAEVAA
                     LVARKGLSVRETEALVRRMLTRKDDEAEHEAPRRDPNIDRLERELTGKLGAKVHLQHG
                     ARGRGKLVINYNSLDELDGILEHIQ"
     gene            2932..3345
                     /locus_tag="PL152_09715"
     CDS             2932..3345
                     /locus_tag="PL152_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HQR49604.1"
                     /translation="MSNQDVQKGRRQALAVVMGQVALGAIVAIACLAGWGTRAALSAL
                     AGAGIGIAATALMAFAMLRHGAGASVGRVALSFFSGWLVKVGFTVAALVVAFRSPGVQ
                     AAPLIAAYAATFAGYWFGAARWGGRQEERTVGVTE"
BASE COUNT          568 a         1161 c         1174 g          525 t
ORIGIN      
        1 ttcgtcgaga agggcgttca gggtacggcc atcgaggaca tctgcaccgc atccggcgcg
       61 agcgtcggca gcctctatca cctgttcgac ggcaagaaca gcgtcgctgc cgccgtgtat
      121 ctcgacgcgc tggaggattt ccaggcagca gtcgcgggga gcgttgccgc cgacatgggc
      181 gcacgtgaag gcgtcctcgc gatcgtcgcg gcacacatcc gctgggtcga ggagaacgcg
      241 gctcgcgcgc gattcctgca gcaggcgcga cacggagagg tcatcgcagc acgcgcggcg
      301 gacattgccg aggcgaatcg cagatttggc cgggtgctcg cggcctggat ggcgccgcag
      361 gtggccgcag gacgactgcg cgcgctgccg ctggacctgt tcatcgcgct gctgcttgga
      421 cccgcgcagg aatacgtgcg cggccggatg tcgggccgcg acagcaccgc atccgacgat
      481 gcaatacacg agctcggcgc tgcagcgtgg cgctccgtcg gtcgcgacat accggaatcc
      541 gtatcgaggt gaggcggcca tgaacgcgct gcaccgcacg tacacgagcg caccgtctgc
      601 gctggcgtac atgctgcagg cattccggcc gtccccgggg tggcgcagcg aacgcggctg
      661 cccggacatg cggctcacct ggcgcaacta ccgcgtcgac cccgccgccg ccgctcagct
      721 ggccgccatc tgcgggctcg acggcgcgag cgcttccacg cgcggcgcga tcctgttccc
      781 gcaggtgacc ggcttccgcc tcatcatggc catgctgaca cacgcatcct ggccgcttcc
      841 catctggaac gcactgcagg tccgaaaccg catcacgctg cacggtccgc tgcgagcgga
      901 cgagcagtac gcactcgaag tgcgtccctc cggctggcgc gtccttgcca aaggcctgga
      961 aatcgacctg cacacgatcc tcatgcacga aggccagctg cgctgggaga gcgtggtgac
     1021 cttctactat cgcggccgct acggcgatgc caccgaaagc ggtgacagca ggagtgcgcc
     1081 ggcgaattcg cctgccgtcg acatggaaaa cccgagtgcc gagatcgtgc gctggaaggt
     1141 cggcgatggt caccgtcgcg ccttcgcaaa gctcacgggt gactacaacg ggctgcacca
     1201 gtgggacgca tatgcgcgtc gcatgggatt cccggcagcg tttccacacc cgcagcgcgt
     1261 ggtcgggcag gccctcgtgc acctgcccga agcggaccac gcgccactga cgctcgacct
     1321 ctggatcaag ggaccggtgt tcttcggctg cggtctcctg ctgcgccact ggcccgcgac
     1381 cggcggggcc gcccgcacct ttgcggtgtg gctgcaaggc gagtcccgcc cggcgctggt
     1441 cggcacgctg gggggaccga cccctgcaca ggtgcacggc gccggctgat ccagggccca
     1501 actcgcagcg agggccgcgg gtcgttccgc taaacttcag ctcctcgcga gcgccgcgga
     1561 tccatcctct aaactgcgac ctatggcaac caagcgaccc tcgctgggtc ggggcctcga
     1621 ggccctgctc ggcaaaccgg cgacgagtca tgaacccgat ggctcggctc ccgccgcccc
     1681 ccgcgacgag gaactggcgc gcatcccggt ggacctcctg cagcgtggcc gataccagcc
     1741 gcgactcgac atgcggcccg agagcctgca ggaactcgcc gactcgatcc gcgcccaggg
     1801 cgtcgtgcag ccgatcgtga tccgtccgct cgccgtgcag cgcgcgggcg aggagcagcg
     1861 ctacgagatc atcgccggcg agcggcgctg gcgggcggcg cagatggccg gcctgcacga
     1921 cgtgcccgcc gtcatccgcc gcgtgcccga cgaggcggcg gtcgcgatgt cgctcatcga
     1981 gaacatccag cgtgagaacc tcaacccact ggaggaggcc cgggcactcg atcggctgat
     2041 ccgcgaattc gagatgacgc accagacggc cgccgacgcg gtcggccgtt cgcgagccgc
     2101 cgtgagcaat ctcctgcgcc tgctcgagct cgccgaggaa gtgaagtcgc tggtcgagca
     2161 gcggcagctc gagatgggtc acgcgcgcgc cctgctcggc ctcaccgcgc cgcggcagca
     2221 ggcggaggtc gcggcactgg tggcccgcaa gggcctgtcg gtgcgcgaga ccgaggccct
     2281 cgtccgccgg atgctgacgc gcaaggacga cgaggccgag cacgaggcgc cgcgccgcga
     2341 cccgaacatc gatcgcctcg agcgcgagct cacgggcaag cttggcgcga aggtccacct
     2401 ccagcacggc gcgcgcggcc gcggcaagct ggtcatcaac tacaacagcc tcgacgagct
     2461 cgacggcatc ctcgagcaca tccagtagcg cagcacccgg cgcagccagg gttgctggcg
     2521 ataattaaag gctcatttat agagcagcgt aagcacgccg tcaggaagct tcaacagtct
     2581 gtctttgtta gtgaatattt gaatatctgg aatcgtgtcg tgccggagtc ttcaccgggt
     2641 gcgaccggtc aatgacaccg ctgctaacga agggggaggc acgtcgatcg cgcccgtttc
     2701 gagtcgatca tcgcggcgta gaacgagact ccgcaagaac gcaatcctcg gtattccgca
     2761 gccctgtgtt tcattgaagc actgcaaaaa gaccagatat aatttcgggc cttccggagg
     2821 ccacgtcatg cggcgtggcc accccgggta acggtaagaa cgccacccgt ggccggagcg
     2881 cccggcacat gacgtggctt ttttgttgcc cggtgatttg tgagtgaggc cttgagcaac
     2941 caggacgtcc agaagggtcg gcgccaggcg ctggccgtcg tgatgggcca ggtcgcgctc
     3001 ggggcgatcg ttgcgatcgc ctgcctcgcc ggctggggca cccgtgcagc gctgtccgcg
     3061 ctcgcaggtg cggggatcgg catcgcggcg accgccttga tggcgttcgc gatgctgcgg
     3121 cacggcgcgg gggcaagcgt ggggcgggtc gccttgagtt tcttctcggg ctggctcgtg
     3181 aaggtgggat tcaccgtcgc cgcgctggtg gtcgctttcc ggtcgccggg ggtccaggcg
     3241 gcgccgctga tcgcggccta cgccgcgacc ttcgccgggt actggttcgg ggccgcccgc
     3301 tggggcgggc ggcaagaaga acggacggtc ggagtgacgg aataaatggc cggcgaagct
     3361 tccttcagca gcacggaata catcacccac cacctgaccc acgagacggt cggcagcggc
     3421 ttctgggc
//
LOCUS       DAOMFD010000207         3410 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13519_length_3410_cov_25.104918, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000207 DAOMFD010000000
VERSION     DAOMFD010000207.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3410)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3410
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13519_length_3410_cov_25.104918"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..182
                     /locus_tag="PL152_09720"
     CDS             <1..182
                     /locus_tag="PL152_09720"
                     /inference="COORDINATES: protein motif:HMM:NF012329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HQR49605.1"
                     /translation="DCRKAHPECYIKLNAFDSTRGFETVRMSFMVHRPKVEARYRLRR
                     EEGAGRLVHYTLERA"
     gene            222..1136
                     /gene="cbbX"
                     /locus_tag="PL152_09725"
     CDS             222..1136
                     /gene="cbbX"
                     /locus_tag="PL152_09725"
                     /inference="COORDINATES: protein motif:HMM:TIGR02880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbbX protein"
                     /protein_id="HQR49606.1"
                     /translation="MSTDATGRTFDLRALLAESGVGAVLDELDRELVGLAPVKLRIRE
                     IASLLVVDKARAELGVAGAKPSLHMCFTGNPGTGKTTVATRMATILHRLGHVRKGHLV
                     AVTRDDLVGQYIGHTAPKTKEVLKRAMGGVLFIDEAYYLYRPENERDYGQEAIEILMQ
                     VMENQREDLVVILAGYKGRMDTFFQSNPGLASRVAHHIDFPDYSVEELEAIAEKMVTA
                     LGLRLSPAALQALSEYLALRRNQPNFANARSLRNALDRARLRQARRLLEAGSVDAPML
                     TTIEAEDIRASRVFDAARSGTKAEEAAC"
     gene            1130..2191
                     /locus_tag="PL152_09730"
     CDS             1130..2191
                     /locus_tag="PL152_09730"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HQR49607.1"
                     /translation="MLNPGYTTISKFLIQQVPDGPHGRELAALLVDVAAVVKAISAAV
                     GKGPLVGLASLAGSENAQGESQKKLDLLADELFARGVEWSGLVGAIASEERDDVHFVE
                     GNGRLALVFDPLDGSANLDINLPVGSIFSVLALPEGTRDPAAVLQPGARQLAAGYAIY
                     GPSTQVVLTVGRGTHGFTLDREIGNFVLTHPDIRIPEEGSDYLINASNRRFWEPPVAR
                     YVDECKAGREGPRGRDFNTRWVASMVADVHRVLLRGGIYLYPRDQKEANREGRLRLLY
                     EANPMAMIVEQAGGMASTGRGRILDVTPQRIHQRVPVILGSRAEVTRVERYHAEFDRG
                     EDKPYSSPLFGERSLFRSG"
     gene            2214..3089
                     /locus_tag="PL152_09735"
     CDS             2214..3089
                     /locus_tag="PL152_09735"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002212300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008974 - phosphoribulokinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HQR49608.1"
                     /translation="MSARHPIIAVTGSSGAGTTTVKTTFEWIFRREKIDAAIVEGDAF
                     HRYDRAEMRQKMKDAEAQGNKNFSHFGPEANVFGELEALFRSYSLSGKGRSRTYIHDA
                     DEAAKYGGQPGTFTPWQDVGEGSDLLFYEGLHGAVKTDENDVARYPDVLIGVAPSINL
                     EWIQKLHRDRRTRGYSTEAVVDTILRRMPDYVNYIVPQFSRTHINFQRVPTVDTSNPF
                     IAQDIPTADESFLIIRFANPRGIDFPYLLSMIHDSFMSRPNTIVVPGGKLPLAMQTIL
                     TPLILRLMDTKKRAY"
     gene            3185..>3410
                     /locus_tag="PL152_09740"
     CDS             3185..>3410
                     /locus_tag="PL152_09740"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HQR49609.1"
                     /translation="MDTTPRGLTTAEARARLARHGPNVLPSAPPPSRFAIALRQFKSP
                     LIYVLLAAAVVAMALGDWGDAAFIGIVLAVN"
BASE COUNT          579 a         1191 c         1133 g          507 t
ORIGIN      
        1 gcgactgccg caaggcgcac ccggagtgct acatcaagtt gaacgcgttc gactcgacgc
       61 gtggcttcga gacggtacgc atgtcgttca tggtccaccg gcccaaggtc gaggcccgct
      121 accggctgcg tcgcgaagag ggtgccggcc gcctcgttca ctacacgctc gagcgggcgt
      181 gaggagcacg gggcagccgc ctgcttcccg gagtccatga catgagcacg gacgccaccg
      241 gccggacctt cgatctccgg gcgctgctcg ccgagtccgg cgtcggcgcc gtgctcgacg
      301 agctcgatcg cgaactcgtg gggctcgcgc cggtgaagct gcgcatccgc gagatcgcct
      361 cgctgctggt ggtggacaag gcgcgcgccg aactcggtgt ggcgggcgcg aagccctcgc
      421 tgcacatgtg cttcaccggc aaccccggga ccggcaagac gaccgtggcc acccgcatgg
      481 cgacgatcct gcatcgcctc ggccacgtgc gtaaggggca cctcgtggcg gtcacccgcg
      541 acgacctcgt cggccagtac atcggacaca cggcgccgaa gaccaaggaa gtgctgaagc
      601 gcgccatggg cggcgtgctg ttcatcgacg aggcgtacta cctctaccgg cccgagaacg
      661 agcgcgacta cggccaggag gcgatcgaga tcctgatgca ggtgatggag aaccagcgcg
      721 aggacctggt cgtgatcctc gccggctaca agggccgcat ggacacgttc ttccagtcga
      781 acccgggcct cgcgtcgcgc gtcgcgcacc acatcgactt tccggactac agcgtcgagg
      841 agctggaggc gatcgcggaa aagatggtga ccgcactcgg actgcgcctc tcgccggccg
      901 cgctccaggc gctgtcggaa tacctcgcgc tgcgccgcaa tcagccgaat ttcgcgaatg
      961 cccgttccct gcgcaatgca ctggaccgcg cgcggctgcg ccaggcgcgg cgcctgctcg
     1021 aggccggcag cgtggatgcg ccgatgctca ccacgatcga ggcggaagac atccgcgcga
     1081 gccgagtttt cgacgcggcc cgcagcggga cgaaagccga ggaggccgca tgctgaaccc
     1141 gggctatacg acgatctcga aattcctgat ccagcaggtg ccggacggcc cgcacgggcg
     1201 cgaactggcg gcattgctcg tggacgtcgc tgccgtggtg aaggcgatct ccgcggcggt
     1261 cggcaagggc ccgctcgtgg ggctcgccag cctcgccgga tccgagaacg cgcagggcga
     1321 gtcgcagaag aagctcgacc tgctcgccga cgagctgttc gcgcgcggcg tcgagtggag
     1381 cggcctggtc ggtgcgatcg cctccgagga acgggacgac gtgcacttcg tcgagggcaa
     1441 cggcaggctt gcactcgtgt tcgacccgct cgacggttcg gccaacctcg acatcaacct
     1501 gccggtcggg tcgatcttct cggtgctcgc gctgcccgag ggcacgcgcg atccagcggc
     1561 agtcctgcaa ccgggagcga ggcagctggc tgctggctac gccatctacg ggccgtccac
     1621 gcaggtcgtg ctcaccgtcg gccgcggcac gcacggattc acgctcgacc gggagatcgg
     1681 caacttcgtg ctgacgcacc ccgacatccg catcccggag gaggggtcgg actacctcat
     1741 caacgcgtcg aaccgccgct tctgggagcc cccggtcgcg cgctacgtgg acgagtgcaa
     1801 ggccggccgc gagggcccgc gcgggcgcga cttcaacacg cgctgggtcg cctcgatggt
     1861 cgctgacgtg caccgcgtgc tgctgcgcgg cggcatctac ctctatccgc gtgaccagaa
     1921 ggaggcgaac cgcgagggac gcctgcgcct gctctacgag gcgaacccca tggccatgat
     1981 cgtcgagcag gcgggcggca tggcgtccac cgggcgaggc cggatcctcg acgtgacacc
     2041 gcagcgcatc caccagcgag tgccggtgat cctcggctcg cgcgcggagg tcacgcgcgt
     2101 cgagcgctac cacgccgagt tcgaccgggg cgaggacaag ccctacagct cgccgttgtt
     2161 cggcgagcgt tccctgttca ggagcggctg agcgccgcgc ccggagtatc gccatgtccg
     2221 caaggcaccc catcatcgcc gtgaccggtt cctccggagc gggaaccacc accgtcaaga
     2281 cgaccttcga gtggatattc cgccgcgaga agatcgatgc cgcgatcgtc gagggcgacg
     2341 cgttccaccg ctacgaccgc gccgagatgc gccagaagat gaaggacgcg gaggcccagg
     2401 gcaacaagaa cttcagccac ttcggcccgg aggcgaacgt cttcggcgag ctcgaggcgc
     2461 tgttccgcag ctactcgctc tccggcaagg gccgctcgcg cacctacatc cacgacgccg
     2521 acgaggccgc gaaatacggc ggccagcccg gcaccttcac gccctggcag gacgtcggcg
     2581 aaggctcgga cctgctgttc tacgaagggc tgcacggcgc ggtgaagacc gacgagaacg
     2641 acgtcgcgag gtaccccgac gtgctgatcg gcgtggcgcc gagcatcaac ctcgagtgga
     2701 tccagaagct gcaccgggac cgtcgcacgc gcggctactc gaccgaggcg gtggtcgaca
     2761 cgatcctgcg ccgcatgccc gactacgtga actacatcgt cccgcagttc tcgcgcacgc
     2821 acatcaattt ccagcgcgtg ccgaccgtcg acacctccaa cccgttcatc gcgcaggaca
     2881 tcccgacggc ggacgagagc ttcctgatca tccgcttcgc gaatccgcgc ggcatcgact
     2941 ttccgtacct gctgtcgatg atccacgact cgttcatgtc gcgcccgaac accatcgtcg
     3001 tgcccggcgg caagctgccg ctcgcgatgc agaccatcct gacgccgctg atcctgcggc
     3061 tgatggacac caagaagcgc gcgtattgaa ataggggaca ttcccctttt tccggacgga
     3121 aaaacgggga atgtccccta tttcccctat tcccttccgc ggggcctcgg cctgtaatgg
     3181 cccggtggac acgacaccac gcggactgac cacggcagag gcgcgcgccc ggctcgcacg
     3241 tcacgggccg aacgtgctgc cgtcggcgcc gccaccgagc cggttcgcga tcgcgctccg
     3301 gcagttcaag agcccgctca tctacgtgct gctcgccgcc gccgtcgtgg cgatggcgct
     3361 gggcgactgg ggcgacgccg cgttcatcgg catcgtgctc gccgtgaact
//
LOCUS       DAOMFD010000208         3392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13652_length_3392_cov_30.816901, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000208 DAOMFD010000000
VERSION     DAOMFD010000208.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3392
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13652_length_3392_cov_30.816901"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..257
                     /locus_tag="PL152_09745"
     CDS             <1..257
                     /locus_tag="PL152_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HQR49610.1"
                     /translation="EIMGPIEQVAAKVFPGVPVLPLFQAGATDGQFLGAAGIPTYGIG
                     VLFFDPDLGGIHGLNERIRVQSLYDGRDFLYELVRVYSAQ"
     gene            complement(339..1823)
                     /locus_tag="PL152_09750"
     CDS             complement(339..1823)
                     /locus_tag="PL152_09750"
                     /inference="COORDINATES: protein motif:HMM:TIGR01317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HQR49611.1"
                     /translation="MGKPTGFIEYLRELPVDRAPLTRVADWNEFHPHLEERRLRNQGA
                     RCMDCGVPFCHTGKLVSGMASGCPINNVIPEWNDLVYRGLWREALDRLHMTNNFPEFT
                     GRVCPAPCEGSCVLGISDPPVTIKSIEAEIIDRGWDEGWVLPEVPKLRTGKKVAVIGS
                     GPAGLAAAAQLNRAGHEVTVLEREDRPGGLLMYGIPNMKLDKREVVERRIALLEQEGV
                     RFLCNASVGENVEAQLLLRDFDATVICTGATQPRDLPVEGRGLAGVHYAMEYLTASTK
                     SLLGRGPDSSPIHARGKDVVVIGGGDTGTDCVGTALRQGCRSLVQIEILPRPPLERAA
                     DNPWPEWPKLYKLDYGQEEAEAKFGDDPRVYLTTVKKFVGDAKGQLRELVTVQVKWER
                     NDQGQFVPAEVPGTEKRLPAQLALIAMGFLGPEQSLLKDLDVECDARSNIKAEHGRYA
                     TSLKGVFAAGDCRRGQSLVVWAINEGRGAARECDRYLMGKTDLP"
     gene            complement(1827..>3392)
                     /locus_tag="PL152_09755"
     CDS             complement(1827..>3392)
                     /locus_tag="PL152_09755"
                     /inference="COORDINATES: protein motif:HMM:NF013645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HQR49612.1"
                     /translation="LIHDLKNANRRARVSVKLVAEVGVGTVAAGVAKAHADVVLVSGY
                     DGGTGASPQTSITHAGLPWELGLAETHQTLVLNNLRSRIAVETDGQLKTGRDVAIAAL
                     LGAEEFGFATAPLVSTGCIMMRVCHLNTCPVGVATQDPKLREKFKGKPEHVVNFMRFI
                     AEELREIMAQLGFRTLEDMVGRVDKLEPRRAIAHWKAKGLDFTNLLYSPEVDPDWGRY
                     CQDEQDHGLDKSLDLTVLLDLCRPAIERGEDVVADLPIRNVNRVVGTITGSEITLRHG
                     AKGLPEDTVRLHFRGSAGQSFGAFIPPGMSLSLEGDANDYVGKGLSGGKISVFPPRES
                     TFAAEDNIIVGNVALYGATSGEAYIRGMAGERFCVRNSGVNAVVEAVGDHGCEYMTGG
                     RVVVLGSTGRNFAAGMSGGIAYVLDERGDFPGRVNGQMVDLEKLEQAAEATEVRRLVE
                     RHLQHTSSERARQVLDAWDDFLPKFVKVIPRDYKRMLGAIARAEDQGLAGEEAIMVAF
                     EENARDLSRVGGN"
BASE COUNT          478 a         1209 c         1126 g          579 t
ORIGIN      
        1 ccgagatcat ggggccgatc gagcaggtgg ccgcgaaggt attcccgggc gtgccggtgc
       61 tgccgctgtt ccaggccggc gcgacggacg ggcagttcct cggcgcggcc ggcatcccga
      121 cgtacggcat cggcgtgctg ttcttcgatc cggacctcgg tgggatccac ggcttgaacg
      181 agcgcatccg cgtgcagtcg ctctacgacg ggcgcgactt cctctacgag ctggtgcggg
      241 tctactccgc gcagtaggaa tcggggacat tcccctgatt ttccccgggg acgcggccgg
      301 tgcccgtccc gcggaaaaca ggagaatgtc cccaattctt atggcaggtc ggtcttgccc
      361 atcaggtagc gatcgcactc gcgggccgcg ccgcggccct cgttgatcgc ccacacgacc
      421 aggctctggc cgcgccggca atcaccggcg gcgaagacgc ccttgagact cgtcgcgtac
      481 ctgccgtgct cggccttgat gttgctgcgg gcgtcgcact cgacgtccag gtccttgagc
      541 agcgactgct cggggccgag gaaccccatc gcgatcaagg cgagctgcgc ggggagcctc
      601 ttttcagttc cggggacctc cgctgggacg aactgcccct ggtcgttgcg ctcccacttc
      661 acctgcaccg tgacgagttc cctgagttgg cccttcgcat ccccgacgaa cttcttcacc
      721 gtggtgaggt acacgcgcgg gtcgtcgccg aacttcgcct cggcctcttc ctgcccgtag
      781 tccagcttgt acagcttcgg ccactccggc cacggattgt ccgcggcccg ctcgagcggc
      841 ggacgcggca ggatctcgat ctggacgagg ctgcggcagc cctgccgcag cgcggtgccg
      901 acgcagtcgg tgccggtgtc gccgccgccg atcaccacga cgtccttgcc gcgcgcgtgg
      961 atcgggctcg agtccggacc gcgacccagc agcgacttgg tgctggcggt gaggtactcc
     1021 atcgcgtaat gcacgcccgc gagaccgcgc ccttcgacgg gcaggtcgcg cggctgcgtg
     1081 gcccccgtgc agatcaccgt cgcgtcgaaa tcgcggagca gcagctgcgc ctcgacgttt
     1141 tcaccgacac tcgcattgca caggaagcgg acgccttcct gctcgagcag cgcgatgcgg
     1201 cgctcgacga cctcgcgctt gtcgagcttc atgttcggga tgccgtacat gagcaaaccg
     1261 cccggccggt cctcgcgctc gagcacggtt acctcgtggc cggcgcgatt cagctgcgcg
     1321 gccgccgcga gccccgccgg ccccgacccg atgaccgcca ccttcttgcc cgtgcgcagc
     1381 ttcggcactt cgggcaggac ccagccctcg tcccagccgc gatcgatgat ctccgcctcg
     1441 atgctcttga tcgtgacggg cggatcgctg atgccgagca cgcacgagcc ctcgcagggc
     1501 gcagggcaca cgcgtccagt gaattccggg aagttgttcg tcatgtgcag gcggtcgagc
     1561 gcctcgcgcc acaggcctcg atagacgagg tcgttccact ccgggatcac gttgttgatg
     1621 gggcagccgg aggccatgcc gctcacgagc ttgcccgtgt ggcagaacgg cacgccgcag
     1681 tccatgcatc gcgcgccctg gttgcgcagc cgccgctcct cgaggtgggg gtggaactcg
     1741 ttccagtccg cgacgcgcgt cagcggcgcg cgatcgaccg gcagctcgcg gaggtactcg
     1801 atgaatcccg tgggcttgcc catggctcag ttaccgccga cccgcgacag gtcgcgggcg
     1861 ttttcttcga aggccaccat gatcgcttcc tcacctgcga gtccctggtc ctcggcccgg
     1921 gcgatcgcgc cgagcatgcg cttgtagtcg cgcggaatga ccttgacgaa tttcggcagg
     1981 aaatcgtccc aggcgtcgag cacctggcgc gcccgctcgc tggaagtgtg ctgcaggtga
     2041 cgttcgacca ggcggcgcac ctcggtcgcc tccgccgctt gctccagctt ctcgagatcc
     2101 accatctggc cgttgacccg gcccgggaag tcgccgcgct cgtcgagcac gtaggcgatg
     2161 ccgcccgaca tgccggccgc gaagttgcgg cccgtggacc cgagcaccac gacgcgtcca
     2221 cccgtcatgt attcgcaacc gtggtcgccc acggcctcga cgaccgcgtt cacgccgctg
     2281 ttgcgcacgc agaagcgctc gcccgccatg ccgcggatgt acgcctcgcc gctcgtggcg
     2341 ccgtagagcg cgacattgcc gacgatgatg ttgtcctcgg ccgcgaacgt ggactcacgc
     2401 ggcgggaaca ccgagatctt gccgcccgac aggcccttgc cgacgtagtc gttggcatcc
     2461 ccctcgagcg agagcgacat gcccggcggg atgaacgcgc cgaagctctg cccggccgac
     2521 ccgcggaagt gcaggcgcac cgtgtcctcc ggcaggccct tcgcgccgtg cctgagcgtg
     2581 atctcgctgc cggtgatcgt gccgaccacg cgattgacgt tgcggatcgg aaggtccgcg
     2641 accacgtcct cgccgcgctc gatcgcggga cggcacaggt cgagcaggac ggtgaggtcc
     2701 agcgacttgt cgaggccgtg atcctgctcg tcctggcagt accggcccca gtccgggtcc
     2761 acctcgggcg agtagaggag gttggtgaag tcgaggccct tcgccttcca gtgcgcgatc
     2821 gcgcggcgcg gctcgagctt gtcgacgcgc ccgaccatgt cctcgagcgt gcggaagccg
     2881 agctgcgcca tgatctcgcg cagttcctcg gcgatgaagc gcatgaagtt gacgacgtgc
     2941 tcgggcttgc ccttgaactt ctcgcgcagc ttcgggtcct gcgtcgccac gcccaccggg
     3001 caggtgttca ggtggcagac gcgcatcatg atgcagccgg tgctgacgag cggcgccgtg
     3061 gcgaagccga attcctcggc gccgagcagc gcggcgatcg ccacgtcgcg acccgtcttg
     3121 agctggccgt ccgtctccac ggcgatgcgg ctgcgcaggt tgttgagcac cagcgtctgg
     3181 tgcgtctcgg cgagccccag ctcccagggc aggcccgcgt gcgtgatcga cgtctgcggc
     3241 gaggcgccgg tgccgccgtc gtagccgctc accagcacca cgtccgcgtg cgccttggcc
     3301 acgccggccg cgacggtgcc cacgcccact tcggccacga gcttcacgct cacgcgtgcg
     3361 cgccggttcg cgttcttcag gtcgtggatc ag
//
LOCUS       DAOMFD010000209         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13676_length_3389_cov_33.184763, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000209 DAOMFD010000000
VERSION     DAOMFD010000209.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13676_length_3389_cov_33.184763"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..636)
                     /locus_tag="PL152_09760"
     CDS             complement(<1..636)
                     /locus_tag="PL152_09760"
                     /inference="COORDINATES: protein motif:HMM:NF018561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HQR49613.1"
                     /translation="MKPSIHRVIGLALGAAGMIVGAAGAAHAAGGVSVQEATSIATDA
                     YVYGYSLITTDVTRIQMSNVATAGMLQAPMNQFANVPRYPPGDYRGVSAPNADTLYSL
                     AWLDLKEPQVFSHPDMGNRFHLFPMVDLWMTIFASPGSRTGDGKAANYLITGPGWKGE
                     VPKGMTHIAAATRYMVILGRTYADGTEADYKAVNALQAQYKITPLSAWGKPY"
     gene            complement(850..1482)
                     /locus_tag="PL152_09765"
     CDS             complement(850..1482)
                     /locus_tag="PL152_09765"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HQR49614.1"
                     /translation="MSKRPRVLVADDHRIVIAGLEALLADRYEITARASDGRALLELA
                     RGADADFIVADISMPGLNGIEALEAMRREGIETPVIFLTMHAEPAYARRALEAGAAGY
                     VLKHEATQELLQALEVVAQGGVFVSAELAAEVLGGARPERDPASGLTPRQREILQLLV
                     DGRSAKEIARLLDISHRTVEYHKYQAMQSLGVESNAELIQLAVRLGIVGH"
     gene            complement(1479..3278)
                     /locus_tag="PL152_09770"
     CDS             complement(1479..3278)
                     /locus_tag="PL152_09770"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HQR49615.1"
                     /translation="MILPWRIVTTALLGLCAGLAMAGTDGPPDEPPAGPVAQRVLIIH
                     SFGRNVAPYDVIAATIRRNVASRAESPVVFLEATLDAGRPVSAHEEQAFVDYLRVRYS
                     EPPIALVVTTGAAAAKFVMQHRDELFPDVPVVFAGIDYRVMPPVSQLRPGDAAIPSYL
                     DPARIYDNILRLQPDTGTIALVAGDSPVEHYWRKVFEEASAPYADRVRFDWYDGLSVE
                     EMKRRIKALPANSAVIYGIVMVDGAGIPFERLAALDELLEQSPVPIYSVFGSEIGRGA
                     VGGPYLPEADSGAISADIALERLESRERPPPVMHKIAMAAPIYDWRALERWRIPESRL
                     PPGSEVRYRAPSLWAEHGTGIVTALAVMLAQALLIAGLLFQRARRRSAEREARMLGGR
                     LISAYEDEGRRLARELHDDVTQRLAGLSMEAATLSRHGDMQDRTAAELAISRELSGLS
                     REVHAMSYRLHPSVIDDLGLVEALRVECARLAGRGEVKVEFGAEPGIEAIRGPAALCL
                     FRVAQEALSNAARHAGARRIEVRVWPEGGGAGLGIVDDGVGFEPTAPRERASLGLASM
                     RERVMLLGGRYKLESRRGKGTRVTAWVPSGPAT"
BASE COUNT          488 a         1205 c         1184 g          512 t
ORIGIN      
        1 gtagggcttg ccccaggccg acaacggcgt gatcttgtac tgcgcctgca gcgcgttgac
       61 cgccttgtag tcggcttcgg tgccgtcggc ataggtacgg ccgaggatca ccatgtagcg
      121 ggtcgccgcc gcgatgtgcg tcatgccctt cggcacttcg cccttccagc ccgggccggt
      181 gatcaggtag ttcgccgcct tgccgtcgcc cgtgcggctg ccggggctgg cgaagatcgt
      241 catccacagg tcgaccatcg ggaacaggtg gaagcggttg cccatgtccg gatgactgaa
      301 gacctgcggt tccttcaggt cgagccaggc gagcgagtac agcgtatccg cgttcggcgc
      361 ggaaacgccg cgatagtccc ccggcggata gcgcgggacg ttcgcgaact ggttcatcgg
      421 cgcctgcagc atgccggccg tggccacgtt cgacatctgg atgcgcgtga cgtcggtcgt
      481 gatgagcgag tagccgtaga cgtaggcatc ggtggcgatg ctggtcgctt cctgaaccga
      541 gaccccgccg gcggcatggg ccgcgccggc ggcgccgacg atcatgccgg ccgcgccgag
      601 cgccaggccg atcacacggt gaatgcttgg tttcatgtcc ggttacgctc caaggtgttg
      661 gggatgactg cgcggggccc gcggccgccg caccgacggt gcgggctcac ggtcgccgca
      721 cgcttcggta cccggattgt catcgaatgg ctgcgggagt cacctcgtag gactacgagg
      781 tcggggggcg caactcggga tcaagatcgg ggctggggtc gggggctgga gccgggccct
      841 ggccgccggt cagtgcccga cgatgcccaa ccgcacggcc agctggatca gctcggcgtt
      901 gctctcgacg ccgagcgact gcatggcctg gtacttgtgg tactcgaccg tccggtgcga
      961 gatgtcgagc agccgcgcga tctccttggc cgaccgcccg tcgaccagca gttgcaggat
     1021 ctcgcgctgg cgcggggtca gccccgacgc cgggtcgcgc tcgggccgcg caccgccgag
     1081 cacctcggcc gcgagctcgg cgctcacgaa gaccccgccc tgcgcgacga cctcgagtgc
     1141 ctgcagcagt tcctgcgtcg cctcgtgctt cagcacgtaa ccggcggcgc ccgcttcgag
     1201 cgcgcgacgg gcgtacgcgg gttccgcgtg catggtcagg aagatcaccg gcgtctcgat
     1261 accttcgcgc cgcatcgcct cgagcgcctc gatgccgttc aaccccggca tcgagatgtc
     1321 ggcgacgatg aaatccgcgt cggccccgcg cgccagctcg agcagcgcac gaccgtcgga
     1381 cgcacgagcg gtgatctcgt agcggtccgc gagcagagcc tcgagtccgg caatcacgat
     1441 ccggtggtcg tcggccacca gcacgcgcgg tcgcttgctc atgtcgccgg ccccgagggc
     1501 acccaggcgg tcacccgcgt gcccttgccg cgccggctct ccagcttata gcggccgccg
     1561 agcagcatca cgcgctcgcg catgctggcg agcccgagcg acgcgcgctc ccgcggtgcc
     1621 gtgggctcga agccgacacc gtcatcgacg atgccgagcc cggcgccgcc gccttctggc
     1681 cagacccgca cctcgatccg cctggccccg gcgtggcgcg ccgcattgct gagcgcctcc
     1741 tgggctacgc ggaacaggca cagcgccgcc gggccgcgta tcgcctcgat gcctggttcg
     1801 gcaccgaatt ccaccttgac ctcgccgcgg cccgcgagcc gcgcgcactc gacgcgcagc
     1861 gcctccacga gcccgaggtc gtcgatcacc gacgggtgca ggcgatacga catcgcgtgc
     1921 acttcacggc tgagccccga gagctcgcgg ctgatcgcga gctcggcggc ggtgcggtcc
     1981 tgcatgtccc catggcgcga gagcgtcgcc gcctccatcg agagcccggc gaggcgctgc
     2041 gtgacgtcgt cgtgcagttc gcgggcgagt cgccggccct cgtcctcgta cgccgaaatg
     2101 agccggccgc cgagcatccg cgcctcgcgc tcggcgctgc gccggcgtgc gcgctggaac
     2161 agcagccccg cgatcaggag cgcctgggcc agcatcactg cgagcgccgt cacgatcccg
     2221 gtgccgtgct cggcccacag cgagggggcg cggtagcgga cctcgctgcc cggcggcagg
     2281 cgcgactcgg ggatccgcca gcgctcgagc gcacgccagt cgtagatcgg cgccgccatc
     2341 gcgatcttgt gcatgaccgg cggtggccgc tcacggctct cgagtcgctc gagcgcgata
     2401 tcggcggaaa tcgcgcccga gtcggcctcc ggcaggtacg gcccgccgac ggcgccgcgg
     2461 ccgatttcgc tgccgaatac cgagtagatc ggcacgggcg attgctccag cagttcgtcg
     2521 agcgcggcca gtcgttcgaa tggaatgccg gcgccgtcca ccatcacgat cccgtagatc
     2581 accgcgctgt tcgcgggcag cgccttgatc ctgcgcttca tctcctcgac cgagaggccg
     2641 tcgtaccagt cgaagcgcac ccggtcggcg tacggggcac tcgcttcctc gaagaccttg
     2701 cgccagtagt gctcgaccgg cgagtcgccg gccaccaggg cgatcgtgcc ggtgtcgggc
     2761 tgcaggcgca ggatgttgtc gtagatgcgc gcaggatcga ggtacgacgg gatcgccgcg
     2821 tcgcccggcc gcagctggct caccggcggc atcacccgat agtcgatgcc ggcgaacacg
     2881 accggtacgt ccgggaacag ctcatcgcga tgctgcatca cgaacttcgc cgccgcggcg
     2941 cccgtcgtca cgaccagggc gatcggtggc tcggagtagc gcacgcgcag gtagtcgacg
     3001 aaggcctgct cctcatgggc gctgacgggg cggccggcgt cgagcgtcgc ttcgaggaac
     3061 accacgggac tctcggctct cgatgcgacg ttgcgccgga tggtcgcggc gattacgtcg
     3121 tagggagcga cgttgcgtcc aaacgagtgg atgatcagca cgcgctgcgc gacgggtccc
     3181 gcgggcggct cgtccggcgg cccgtccgtt ccggccatcg ccagccccgc acacagcccg
     3241 agcagggccg tcgtgacgat ccgccaggga aggatcaagg ttcggcgcga acccgattga
     3301 agtgggcgac gcgacggctg cgccgattgc acagccgtcg cgctggcccg cacactacct
     3361 ggcctggacg accgccggcg gcgaccacg
//
LOCUS       DAOMFD010000210         3389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13677_length_3389_cov_30.416917, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000210 DAOMFD010000000
VERSION     DAOMFD010000210.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3389
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13677_length_3389_cov_30.416917"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="PL152_09775"
     CDS             complement(<1..311)
                     /locus_tag="PL152_09775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49616.1"
                     /translation="MSAQHENQDRVPESVLAGHTPVMQQYLRIKAQHPDVLLFYRMGD
                     FYELFFDDARRAARVLDITLTARGQSGGEPIPMAGVPVQTVDTYLARAVRRGESVAIC
                     E"
     gene            complement(503..1102)
                     /locus_tag="PL152_09780"
     CDS             complement(503..1102)
                     /locus_tag="PL152_09780"
                     /inference="COORDINATES: protein motif:HMM:NF024978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4136 domain-containing protein"
                     /protein_id="HQR49617.1"
                     /translation="MSYPRPRIAAAALVACAMLAGCTSTPSVRSDYDKAADFGKYRTF
                     GFVDQTDQAKSLVMQELEAAAAREMETRGYRLAEQPDLVVNFTGKLEDRTDVQSVPGP
                     YYGPTWGYGGWYGAPYGAWGYGGSQVTTRHYKVGELVMDIVDREKRMVVFQAGLERTV
                     TKEMQQHRSQALTQAVKLLFGTYPFVAGQSGPVAPVEKK"
     gene            complement(1118..1594)
                     /locus_tag="PL152_09785"
     CDS             complement(1118..1594)
                     /locus_tag="PL152_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49618.1"
                     /translation="MKKTLLALSAMAALCAAGGAIAQTSGNMTDQEQLLVSQIQTDKR
                     AVVLKAMNLTDQEVAVFTPIYDEYQAAMKQHYSRGSELLNKYASNYDSMTDAAAKDLL
                     KEVFEQREERTDTLKKYAKKMSGKLPATKVLRFVQIENKLNTLLDWQAAQVIPLVR"
     gene            complement(1651..2124)
                     /locus_tag="PL152_09790"
     CDS             complement(1651..2124)
                     /locus_tag="PL152_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49619.1"
                     /translation="MKLVTLALAFVVTAFPLASVAQDTSGTAADEQVLLKQIQTNKRG
                     VFADNLKLTESESKAFWPVYDQYEADLKKLNDRFLQLINDYAAKYDTLTDEQAAAMLK
                     ERLGIDRKRTDLRVKYCERIGKVLPGRKALRFAQLESRIDNLAMRNLYTVIPLAR"
     gene            2209..2709
                     /locus_tag="PL152_09795"
     CDS             2209..2709
                     /locus_tag="PL152_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HQR49620.1"
                     /translation="MASPNDAEIRLVAGRVAGHLLGRRERLSVAESCTGGWVAKACTD
                     LPGSSNWFLGGAVAYANELKAGVLGVASGTLERDGAVSEAAVREMAAGALERFGSDVA
                     VAVSGIAGPDGGLPGKPVGTVWFAWAGRRDGRVHVRAAVEHFAGGRDDVRRQAVARAL
                     EGVLDT"
     gene            2706..3260
                     /gene="thpR"
                     /locus_tag="PL152_09800"
     CDS             2706..3260
                     /gene="thpR"
                     /locus_tag="PL152_09800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HQR49621.1"
                     /translation="MTDASVPRAPARRLFFAVWPDEPARLSLAEATKRHVDAGQGRAQ
                     RADQWHLTVEFLGNVPGARLPDVLDAGAAASAPARAFDVAFDRIEYWKRPQVLCLSAS
                     SIPGPLASLVQSLRSELARRGFVPEARPYKAHVTLARKVRQASPAELVDPLRWAVGEL
                     ALVESVTDRSGSRYESLATWPLGG"
BASE COUNT          478 a         1094 c         1194 g          623 t
ORIGIN      
        1 tgctcgcaga tcgcgaccga ctcgccgcgg cgcacggcgc gggcgaggta ggtgtccacc
       61 gtctgcaccg gcacgcccgc catcgggatc ggctcgcctc cggactggcc gcgcgccgtg
      121 agcgtaatgt cgaggacgcg tgccgcccgc cgcgcgtcgt cgaagaacag ttcgtagaag
      181 tcgcccatcc ggtagaacag cagcacgtcg gggtgctgtg ccttgatccg caggtactgc
      241 tgcatcaccg gggtgtgtcc ggcgagcacc gactccggta cccgatcctg attttcgtgc
      301 tgggcgctca aggggtcgct gccggtcgtc tgctcacgca atggagcacg agacggcgct
      361 gctggcggcc ggtccgtgcg aaggtcgtgc atcgtagcgg agcggggccg cacgcggcca
      421 cccgatggtg gtgcacggga ggacgcgtat cagccgcagc gggacagccg gcagcggcac
      481 cgggccgccg ccggccgttg cttcatttct tctcgacggg cgcgaccgga cccgactggc
      541 cggccacgaa cgggtatgtg ccgaacagca gcttgaccgc ctgggtgagc gcctggctgc
      601 ggtgctgctg catctccttg gtcaccgtcc gctcgaggcc ggcctggaag acgaccatgc
      661 gcttctcacg gtcgacgatg tccatcacga gctcgcccac cttgtagtgg cgggtcgtca
      721 cctgtgagcc gccgtacccc catgctccgt acggggcgcc gtaccagccg ccgtagcccc
      781 aggtcggtcc gtagtacggg ccgggcaccg actgcacgtc cgtccggtcc tcgagcttgc
      841 ccgtgaaatt gacgaccagg tcgggttgct cggcaagcct gtaaccgcgg gtctccatct
      901 cgcgagccgc cgcggcctcc agttcctgca tcaccagcga tttggcctgg tcggtctggt
      961 ccacgaaacc gaatgtgcgg tacttgccga agtccgcggc cttgtcgtaa tcggagcgga
     1021 cggagggtgt cgaagtacag ccagcgagca tcgcgcacgc gacgagcgcc gcggctgcaa
     1081 tccgtggtct cgggtatgac atggtcctgt gactccgtta ccggacgagg gggatcacct
     1141 gcgccgcctg ccagtcgagc agggtattca gcttgttctc gatctgcacg aaacgcagca
     1201 ccttggtcgc cggcagcttc cccgacatct tcttggcgta cttcttcagg gtgtccgtcc
     1261 gctcctcgcg ctgctcgaag acttccttga ggaggtcctt tgccgccgcg tcggtcatgc
     1321 tgtcgtagtt cgaggcgtac ttgttcagca gctcgctgcc gcggctgtag tgctgcttca
     1381 tcgccgcctg gtattcgtcg tagatcggcg tgaagacggc gacctcctgg tcggtgaggt
     1441 tcatcgcctt gagcacgaca gcccgcttgt ccgtctggat ctggctgacg agcagctgct
     1501 cctggtcggt catgttgccg ctggtctgcg cgattgcgcc gccggccgcg caaagggctg
     1561 ccatcgccga cagcgccagc agggtcttct tcatggttca gggctccctt aggatgaatg
     1621 taccttcgac cgccgcggac acgagctggt tcagcgcgcg agcgggatga ccgtgtacag
     1681 gttgcgcatc gcgaggttgt cgatccgcga ctcgagctgt gcgaagcgca gcgccttcct
     1741 gcccggcagc accttgccga tgcgttcgca gtacttgact cgcaggtcgg tgcgcttgcg
     1801 atctatgccg agacgctcct tgagcatggc ggccgcctgc tcgtcggtca gggtgtcgta
     1861 cttggccgcg taatcgttga tgagctgcag gaaacggtcg tttagtttct tgaggtccgc
     1921 ctcgtactgg tcgtacacgg gccagaatgc cttgctctcc gattccgtga gcttgaggtt
     1981 gtcggcaaac acgccgcgct tgttggtctg gatctgcttg aggaggacct gctcgtcggc
     2041 ggcggtaccg gaggtgtcct gcgcgacgga ggcgagcggg aacgcggtga cgacgaatgc
     2101 gagcgccagg gtgaccagtt tcatgcgcga cctgtacggt tgtggtaagg aacgggcacg
     2161 gtagggcagg cgcccccatg ctgtccagag gccgctagaa tcctgcgaat ggcttcgcca
     2221 aatgatgccg aaatccgcct ggtcgccggc cgcgtggccg gtcacctgct gggccgccgt
     2281 gaacgactgt cggttgccga atcctgcacc gggggctggg tggccaaggc ctgcaccgac
     2341 ctcccgggca gcagcaactg gttcctcggt ggcgcggtcg catatgccaa cgaactcaag
     2401 gccggcgtgc tgggggttgc cagtggcacc ctggagcgcg acggtgccgt cagcgaggcc
     2461 gcagtgcgcg aaatggccgc cggggcgctc gagcgcttcg gcagcgacgt ggcggtggcg
     2521 gtgagcggga tcgccggacc ggacggtggc ctgcccggca agcccgtcgg caccgtgtgg
     2581 ttcgcctggg cggggcgccg cgacggtcgc gtgcacgtcc gggcggccgt cgagcacttt
     2641 gccggcggtc gtgacgacgt gaggcgacag gccgtggcac gggcgctcga gggagtgctc
     2701 gacacgtgac cgacgcttcg gtcccgcgcg cgccggctcg gcgtctgttt ttcgctgtct
     2761 ggcccgacga gccggcgcga ctgagcctgg ccgaggccac gaagcgccac gtggatgcag
     2821 gccagggccg ggcgcagcga gccgaccaat ggcacctgac cgtggagttc ctcggcaacg
     2881 tgcccggggc gcgactgccc gacgtgctcg atgccggggc tgcggcgagc gctcccgcac
     2941 gtgcgttcga cgttgctttt gaccggatcg agtactggaa gcggccgcag gtgctgtgcc
     3001 tgtcggcctc atcgatcccc gggccgttgg cgtcactggt gcagtcattg cgctcggagc
     3061 tggcccggcg tggcttcgtg ccggaggcgc ggccgtacaa ggcgcacgtg accctggcgc
     3121 gaaaggtgcg tcaggcgtcg ccggcggaac tcgtcgatcc attgcgctgg gcggtcggcg
     3181 aactggccct cgtcgagtcg gtcaccgatc ggtcgggatc gcgctacgag tcgcttgcga
     3241 cctggcctct cggcggatga ccggtccgcg ccgggagcgg gctcacgcca tgagcctgcc
     3301 cggtgagaaa ttgctgattc gcatgaattt ttcgcccggc gggggcggtg acgctgtgga
     3361 ataatgcgcc ccttcccgga tccccacga
//
LOCUS       DAOMFD010000211         3387 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13687_length_3387_cov_36.320228, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000211 DAOMFD010000000
VERSION     DAOMFD010000211.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13687_length_3387_cov_36.320228"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..397)
                     /locus_tag="PL152_09805"
     CDS             complement(<1..397)
                     /locus_tag="PL152_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HQR49622.1"
                     /translation="MPQMNMIQALNSAMDIMLARDPNVVVLGEDIGYFGGVFRVTDGL
                     QRKYGEHRVLDTPIAEGAIVGAAIGMGVNGLRPVAEVQFADYIYPAYDQIVSELARLR
                     YRSAGEFFAPVTIRTPCGGGIRGGQTHSQS"
     gene            complement(401..1639)
                     /locus_tag="PL152_09810"
     CDS             complement(401..1639)
                     /locus_tag="PL152_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQR49623.1"
                     /translation="MPRRSRLHIPHAHSRPGEAPDFSYLETSPAGAVARPDANARVRD
                     IGNLSVELVRVLDDRHRAVGPWHPHLDPSELQVGLRHMLLTRIFDDRMQRAQRQGKTS
                     FYMRSLGEEAVSVAQAMALRPGDMLFPSYRNQGLFIARGMRLVDMMCQLLSNTRDMCK
                     GRQLPVMYHWVAGRIFSISGNLTTQFPQAVGWAMASAIKGEDDIASTWIGEGSSAEAD
                     FHHAMLFASVYQAPVILNIVNNQWAISTFQGFAGGEQRSFAARGPGYGMAGVRVDGND
                     FLAVHSVTQWAAERARTGAGPTLIEHVTYRGAAHSTSDDPSRYRPKDDYEKWPLGDPV
                     ERLKGHLIALGEWSDVRHAALAKELEQHVTECWKEAVQYGTLNDGPRLNRDLMFEDVF
                     KEMPAHLRKQREQLRAEQGG"
     gene            1766..2245
                     /locus_tag="PL152_09815"
     CDS             1766..2245
                     /locus_tag="PL152_09815"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HQR49624.1"
                     /translation="MEETSRPQLDRMDRRILDLLQREGALPVAEVATRTGISTTTCWR
                     RIQQLEQAGVIRGRVALLDRAALGLDVTIFAHVKLSTQGRDAIAAFAEAIRERPEVLD
                     CYTTMGEWDFMLRVVTKDIKAFEAFYLDHLSKLPNVQSINSSVAVTVIKESTVLPIT"
     gene            2289..2861
                     /locus_tag="PL152_09820"
     CDS             2289..2861
                     /locus_tag="PL152_09820"
                     /inference="COORDINATES: protein motif:HMM:NF013981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-archaeol synthase"
                     /protein_id="HQR49625.1"
                     /translation="MTLILQLLWLAVPVIVSGLVHLAVMKLDLLPGLRRLPIDGGLTF
                     RGKRVCGDNKTWRGAIVTIATVTASAWALAQLDACCLHLPTLVPFAEQHPLQWGLLLG
                     SGYIIGELPNSFLKRQLDIGPGASGSGLMGRVFWVVDQVDSLAGMLLFVWPVWQPPAQ
                     LVLLLFAIMLVAHPIAAWVMVLFGLKKRVG"
     gene            2929..3114
                     /locus_tag="PL152_09825"
     CDS             2929..3114
                     /locus_tag="PL152_09825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49626.1"
                     /translation="MQIVLFTLVAAVLYLVSDRLLLMLEARAGRRFENRSLYFFGILL
                     VLALVTFALIRRYVPGG"
BASE COUNT          500 a         1160 c         1135 g          592 t
ORIGIN      
        1 ggctctgcga gtgcgtctgc ccgccgcgga tgccgccgcc gcagggcgtg cggatggtca
       61 ccggcgcgaa gaactcgccg gcgctgcgat accgcaaccg cgccagttcg ctcacgatct
      121 ggtcgtaggc cgggtagatg tagtccgcaa attgcacctc tgccaccggt ctgagcccgt
      181 tcacgcccat cccgatcgcg gcgccgacga tcgcaccctc ggcgatcggc gtgtcgagca
      241 cgcgatgctc gccgtacttg cgctgcaggc cgtcggtgac gcggaacacg ccgccgaagt
      301 agccgatgtc ctcgccgagg accacgacgt tcgggtcgcg cgccagcatg atgtccatcg
      361 cggagttgag cgcctggatc atgttcatct gcggcatggc tcagcctccc tgctcggcgc
      421 gcagctgctc gcgctgcttg cggaggtgcg ccggcatctc cttgaacacg tcctcgaaca
      481 tgaggtcacg gttcaggcgc ggtccgtcgt tcagcgtccc gtactgcacg gcttccttcc
      541 agcactccgt cacgtgctgc tcgagttcct tcgcgagcgc ggcatggcgc acgtccgacc
      601 actcgccgag cgcgatcagg tgccctttca ggcgttcgac cgggtcgccg agcggccact
      661 tctcgtagtc gtccttgggc cggtagcgcg acgggtcgtc gctggtcgag tgggccgcgc
      721 cgcgataggt gacgtgctcg atgagcgtcg ggccggcgcc ggtgcgtgcg cgctcggccg
      781 cccactgggt caccgagtgc acggcgagga agtcgttgcc gtccacgcgc acgcccgcca
      841 tcccgtagcc ggggccgcgc gccgcgaagg agcgctgctc gccgcccgca aatccctgga
      901 aggtcgagat cgcccactgg ttgttgacga tgttgaggat caccggggcc tggtacaccg
      961 acgcgaacag catcgcgtgg tggaaatccg cctcggcgct cgaaccctcg ccgatccacg
     1021 tcgaggcgat gtcgtcctcg cccttgatgg ccgacgccat ggcccagccg accgcctgcg
     1081 ggaactgggt cgtgagattg ccggagatcg agaagatgcg gcccgcgacc cagtggtaca
     1141 tcaccggcag ctgtcggccc ttgcacatgt cgcgcgtgtt cgacagcagc tggcacatca
     1201 tgtcgacgag ccgcatgccg cgcgctatga acagcccctg gttgcgatag ctcgggaaca
     1261 gcatgtcgcc ggggcgcagc gccatggcct gcgcgaccga gaccgcctcc tcgcccaggg
     1321 aacgcatgta gaacgaggtc ttgccctgcc gctgcgcgcg ctgcatgcgg tcgtcgaaga
     1381 tgcgcgtgag cagcatgtgg cgcagtccga cctgcagctc cgacgggtcg aggtgcggat
     1441 gccagggccc gaccgcccgg tgccggtcgt cgagcacgcg caccagttcg accgacaggt
     1501 tgccgatgtc gcgcacgcgg gcgttcgcgt cggggcgcgc gacggcaccg gccggcgagg
     1561 tctccaggta gctgaaatcg ggcgcttcgc ccggacgtga gtgtgcgtgc gggatgtgca
     1621 ggcgtgatcg tctgggcatg gcgcgctcga tgcttggggc gtggaacggg atcgaccgta
     1681 ggttaaccgg gcaacgccca agttgttgaa ttcattgcga cgcgctgcaa caatgcggaa
     1741 tgcctgttcc gctttgagtc attgcatgga agagacatcc cgacctcagc tcgaccgcat
     1801 ggaccggcgc atcctcgacc tcctgcagcg cgaaggcgcc ctgccggtcg cagaggtcgc
     1861 aacccgcacc ggcatctcca ccacgacctg ctggcgccgc atccagcagc tcgagcaggc
     1921 cggcgtgatc cgcggccgtg tcgcgctcct cgaccgcgcg gccctcgggc tcgacgtcac
     1981 gatcttcgca cacgtcaaac tctcgacgca gggccgcgac gcgatcgccg cgttcgccga
     2041 ggcgatccgc gagcgtcccg aggtgctcga ctgctacacc accatgggcg agtgggactt
     2101 catgctccgc gtggtcacga aggacatcaa ggccttcgag gcgttctacc tcgaccacct
     2161 ctcgaagctg ccgaatgtgc agtcgatcaa ctcgtcggtc gcggtgacgg tgatcaagga
     2221 atcgacggta ctgcccatca catgacgcgc ggcctctggt aacgtcgtgc gcccacgggg
     2281 agggtgcgat gacgttgatc ctgcaactgc tgtggctcgc ggtcccggtg atcgtgtccg
     2341 gcctcgtgca cctcgcggta atgaagctcg acctcctccc cgggctgcgc cggctgccga
     2401 tcgacggcgg gctcacgttc cgcggcaagc gggtctgcgg ggacaacaag acgtggcgcg
     2461 gcgcgatcgt caccatcgcc accgtgacgg cgagcgcctg ggcgctcgcg cagctcgacg
     2521 cctgctgcct gcacttgccc acgctggtgc cgttcgccga gcagcacccg cttcagtggg
     2581 gcctgttgct ggggtctggc tatatcatcg gcgaactgcc caacagcttc ctgaaacgcc
     2641 agctcgacat cgggcccggc gcgagtggca gcggcctcat gggccgcgtc ttctgggtcg
     2701 tggaccaggt cgacagcctc gccggcatgc tgctgttcgt gtggccggtg tggcagccgc
     2761 cggcgcagct cgtgttgctg ctcttcgcca tcatgctggt cgcgcacccg atcgcggcct
     2821 gggtgatggt cctgttcggg ctcaagaagc gcgtgggttg agggcgcggc gaacgcgagc
     2881 gcaagcgtcc ggtgctacag tcgccaccgg ccggtccgga actcacgcat gcagatcgtc
     2941 ctgttcacgc tcgtcgccgc cgtcctctac ctggtgtccg accggctcct gctcatgctg
     3001 gaagcgcggg ccggacgccg gttcgagaat cgcagcctct acttcttcgg catcctgctc
     3061 gtgctcgcgc tggtgacgtt cgccctgatc cggcgctacg tgccgggcgg ttgaagtcgc
     3121 gaaatctttt tgcagtgcac ttgatccagg tcaagcgcag cagagtccgt tggccgtagt
     3181 cttgcgctcg tacgacgctt cggcgtgaat cggggcgggc ggaccacggc ggtccgtccg
     3241 gctgacaaca aacgcaatcc aggggggcca accgatggcc ggatccgacc cgcagcgcgg
     3301 cggcgatcag cccgagcgca ttccgttctt ccagcgcgtg ctcgacaacc cgttcctgct
     3361 gctgttcctc ggcgtgacca tgccgac
//
LOCUS       DAOMFD010000212         3381 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13729_length_3381_cov_37.034275, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000212 DAOMFD010000000
VERSION     DAOMFD010000212.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3381)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3381
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13729_length_3381_cov_37.034275"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..314)
                     /locus_tag="PL152_09830"
     CDS             complement(<1..314)
                     /locus_tag="PL152_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004879284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HQR49627.1"
                     /translation="MMAVNAAVTLARPLLVKGEPGTGKTQLAEEVAAALGKPLYRWHI
                     KSTTKAQHGLYEYDAVSRLRDSQLGEAKVHDIANYIVKGRLWEAFDSERQAVLLIDEI
                     DKA"
     gene            complement(362..1792)
                     /gene="pyk"
                     /locus_tag="PL152_09835"
     CDS             complement(362..1792)
                     /gene="pyk"
                     /locus_tag="PL152_09835"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HQR49628.1"
                     /translation="MLRRTKIVATLGPATDTPGVLEDVIRHGADVVRVNFSHGEARDH
                     RARVDAVRRAAAAVGKHVAVLGDLQGPKIRIEGFAEGRAILAEGEPFALDPAIGPEDG
                     TDRAVGITYPELARDVQPGDELILGDGQVELRVTGIENERVECRVTIGGEVSDHKGIN
                     RRGGGLSARALTDKDREDVRLAAALDVDYLAISFVRQASDVEEARALLRAAGSDARIV
                     AKIERSEAIPRLEEIIRASDAVMVARGDLGVEVGYAQLTGLQKRILHLARGLERVTIT
                     ATQMMESMIHSPVPTRAEVSDVANAVLDGSDAVMLSGESAVGKYPARAVDAMAEVIEG
                     AEMYQLARNRGGERFEGRLHSVDKAIAHAVMYTANHMDVEAIVALTESGSTPLWMSRI
                     RSDIPIFAFTRHERTQRRVALYRGVYPIPFDVTHTEPAGLYRAISKELLDRGHVAPGD
                     DLILTLGELSGVSGGTNTMKLLKVTT"
     gene            complement(1906..3084)
                     /locus_tag="PL152_09840"
     CDS             complement(1906..3084)
                     /locus_tag="PL152_09840"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004973295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HQR49629.1"
                     /translation="MRVLRMTDLDLKGKRVLIREDLNVPVKNGVVTSDARIRASLPTI
                     KAAHAAGARVILMSHLGRPEEGVPAEEFSLAPVAARISELLGLKVRFERNWLDGVACE
                     PGEIVLVENVRFNKGEKKNKDDLAQKMAGLCDVYVMDAFATAHRAEASTHGVAKFAPV
                     ACAGPLLTGELEALEKALGSPKRPLVAIVGGSKVSTKLTVLENLLGKVDRLIVGGGIA
                     NTFLAAAGQPVGKSLHEAEMVDIARGLLERSKSSGVDIPLPTDVVVAKEFSATAEADV
                     KPASAVGPDEMILDIGPDTAERFAGALKGAGTIVWNGPVGVFEFDQFGEGTRVLAQAV
                     ARSPAFSIAGGGDTIAAIEKYGVGEDISYISTAGGAFLEFLEGKKLPAVEVLEQRAGK
                     "
     gene            complement(3109..>3381)
                     /locus_tag="PL152_09845"
     CDS             complement(3109..>3381)
                     /locus_tag="PL152_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HQR49630.1"
                     /translation="DLTVELSKPAKYDEICAAMKAASEGAMKGVLGYTTEKVVATDFR
                     GESCTSVFDAEAGIALDDTFVKVVAWYDNEWGYSCKVLEMVRVIAK"
BASE COUNT          480 a         1220 c         1121 g          560 t
ORIGIN      
        1 gccttgtcga tctcgtcgat gagcagcacg gcctgccgct cgctgtcgaa cgcctcccac
       61 aggcggccct tgacgatgta gttggcgatg tcgtggacct tcgcctcgcc gagctgggag
      121 tcgcggagcc gcgacacggc gtcgtattca tagagcccgt gctgggcctt ggtcgtggac
      181 ttgatgtgcc agcggtacag cggcttgccg agcgcggcgg ccacctcctc cgcgagctgc
      241 gtcttgccgg tcccgggctc gcccttcacc agcagcgggc gcgccagggt cacggcggca
      301 ttgaccgcca tcatcaggtc ctcggtcgcg acgtagcgct cggtcccctc gaatttcctg
      361 gtcacgtcgt caccttcagc agcttcatgg tgttggttcc gcctgagacg cccgagagct
      421 cgccgagcgt gagtatcagg tcgtcgccgg gggcgacgtg cccacggtcg agcagctcct
      481 tggagattgc gcggtacagc ccggcaggct cggtgtgcgt gacgtcgaac gggatcgggt
      541 acacgccccg gtacagcgcc acgcggcgct gcgtgcgctc gtgccgcgtg aacgcgaaga
      601 tcgggatgtc cgagcggatg cgggacatcc acagcggcgt cgagccggac tcggtcagcg
      661 cgacgatcgc ctccacgtcc atgtggttcg ccgtgtacat cacggcgtgc gcgatcgcct
      721 tgtcgacgct gtgcaggcgc ccctcgaagc gctcgccgcc gcgattgcgc gcgagctggt
      781 acatctcggc gccctcgatg acctcggcca tcgcgtcgac cgcgcgggcg gggtacttgc
      841 cgaccgccga ttcccccgag agcatcaccg cgtcgctgcc atccagcacg gcgttcgcca
      901 cgtccgagac ttccgcgcga gtgggcaccg ggctgtggat catcgactcc atcatctgcg
      961 tcgcggtgat cgtcacgcgc tcgaggccgc gcgcgaggtg caggatgcgc ttctgcagcc
     1021 cggtgagctg cgcatagccg acctcgacgc cgaggtcacc gcgcgcgacc atcaccgcgt
     1081 cgctggcccg gatgatctcc tccaggcgcg gtatcgcctc gctgcgctcg atcttggcga
     1141 cgatgcgcgc atcgctgccg gccgcccgga gcagcgcgcg cgcctcctcg acgtccgacg
     1201 cctggcgcac gaacgagatc gcgaggtagt ccacgtcgag cgcggcggcg agccgcacgt
     1261 cctcgcgatc cttgtcggtc agggccctgg ccgagaggcc gccgccacga cgattgatcc
     1321 ccttgtggtc cgacacctcg ccgccgatcg tcacgcggca ctcgacgcgc tcgttctcga
     1381 tgcccgtgac gcgcagttcg acctggccgt cgccgaggat cagttcgtcc cccggctgca
     1441 cgtcccgcgc gagttccggg tacgtgatcc cgacggcacg gtccgtcccg tcctccggcc
     1501 cgatcgcggg atcgagggcg aagggctcgc cctcggcgag gatggcgcga ccctcggcga
     1561 agccctcgat gcggatcttc gggccctgca ggtccccgag cacggcgacg tgcttgccga
     1621 ccgccgcggc ggcgcggcgc accgcatcca cgcgcgcccg gtgatcccga gcctcgccgt
     1681 gcgagaaatt gacgcggact acgtccgcgc cgtggcggat gacgtcctcg agcacgccgg
     1741 gcgtgtcggt ggcaggtccc agggtggcga cgatcttggt gcggcgaagc atgtggacac
     1801 gagcagcgac gagtggtcag cgcatagtgc gcaatggatc gcgtcgtggc taggggggcg
     1861 gggccaaggc gcgcgggcgg gccgcggcaa atcgtctccg ccgccctact tccccgccct
     1921 ctgctccagc acctcgaccg ccggaagctt cttgccctcg aggaattcga ggaatgcgcc
     1981 gcctgccgtc gagatgtagg aaatgtcctc gccgacgccg tacttctcga tggccgcgat
     2041 ggtgtcgcca ccgcccgcga tcgaaaatgc cgggctgcgc gccacggcct gcgccaggac
     2101 ccgggtgcct tccccgaact ggtcgaactc gaacacgccc accgggccgt tccagacgat
     2161 cgtgccggca cccttcaggg cgcctgcgaa ccgctcggcc gtgtccggac cgatgtcgag
     2221 gatcatctcg tccggcccga cggccgacgc cggcttcacg tccgcctcgg ccgtggccga
     2281 aaactccttc gccacgacga cgtccgtcgg cagcgggatg tcgacgccgc tggacttcga
     2341 tcgctcgagc aggccgcgcg cgatgtcgac catctccgcc tcgtgcagcg acttgccgac
     2401 cggctggccg gccgccgcga gaaacgtatt ggcgatgccg ccgccgacga tgagacggtc
     2461 taccttgccg agcaggttct cgagcacggt gagcttggtg gagaccttcg acccgcccac
     2521 gatcgcgacc agcggtcgct tcgggctgcc gagcgccttc tccagcgcct cgagttcacc
     2581 cgtgagcagc gggccagcac aggccaccgg cgcaaatttt gcaacgccgt gggtgctggc
     2641 ctcggcgcgg tgcgcggtcg cgaaggcatc catgacgtac acgtcgcaga ggcccgccat
     2701 cttctgcgcg aggtcgtcct tgttcttctt ctcgcccttg ttgaagcgca cgttctcgac
     2761 cagcacgatc tcgccgggct cgcacgcgac gccgtcgagc cagttgcgct cgaatcgcac
     2821 cttgaggccg agcagctcgg agatgcgcgc cgcgaccggc gcgagcgaga actcctccgc
     2881 ggggacgccc tcctccggcc ggccgaggtg cgacatgagg atcacccggg cacccgcggc
     2941 gtgcgccgcc ttgatggtcg gcaacgaggc gcggatgcgc gcgtcgctgg tcaccacgcc
     3001 gttcttgacc gggacgttca ggtcctcgcg gatcagcacc cgcttgccct tgaggtcgag
     3061 gtcggtcatg cgaaggactc gcagcatgct cagctccacc attgcgcact acttcgcgat
     3121 cacgcggacc atttccagca ccttgcagga atagccccac tcgttgtcgt accaggcgac
     3181 gaccttgacg aaggtgtcgt cgagcgcgat gcccgcctcc gcgtcgaaca ccgacgtgca
     3241 gctctcgccg cggaagtcgg tcgcgacgac cttctccgtg gtgtagccga gcacgccctt
     3301 catcgcaccc tccgaggcgg ccttcatcgc cgcgcagatc tcgtcgtact tcgcgggctt
     3361 cgacagctcg accgtgaggt c
//
LOCUS       DAOMFD010000213         3344 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_13993_length_3344_cov_36.769231, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000213 DAOMFD010000000
VERSION     DAOMFD010000213.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13993_length_3344_cov_36.769231"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..736)
                     /locus_tag="PL152_09850"
     CDS             complement(<1..736)
                     /locus_tag="PL152_09850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HQR49631.1"
                     /translation="MANIVVLGAGLGGVACAYEMKKKVGKGHQVTVVGSSPYFEFTPS
                     NPWMMVGWRKAEQTRVDLAKPLAAKDIAFVPKMVQAIDAPGSTLKFEDGSTLAYDYLV
                     ITTGPKLAFEEVPGLGPKGFTQSVCTQGHSEQAWQRYQQFAQSPGPVVVGAAAGASCF
                     GPAYESAMILDTDLRRRKIRDRVPMVYVTPEPYIGHMGLGGVGDSKGLMESELRQRHI
                     TWITNAKITGVEDGVMTVAEHAEDGSV"
     gene            complement(756..>3344)
                     /locus_tag="PL152_09855"
     CDS             complement(756..>3344)
                     /locus_tag="PL152_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49632.1"
                     /translation="GYGLTVTEIAASLQAANVVQHAGSLVADGRDVPVQAGEFLADRD
                     QVAQLVVTVHDGRPVYLQDVATVREGPDQPEQYVTFGTGPAAAQKGIQPAGTSPAVTI
                     AISKKPGENAIDVTRAVLKRVEMLRGTYIPDGVEITVTRDYGVTANDKAQKLIQKLAF
                     ATTSVVLLVWLTVGWREAVVVGAAVVITLAATLFASWAWGFTINRVSLFALIFAIGIL
                     VDDAIVVVENIHRHMGLGHAGMGTIIPRAVDEVGGPTILATFTVIAALLPMAFVSGLM
                     GPYMSPIPINASMGMLISLAIALVITPWLSKRMLSHAHAAHGGGARLHQFFVRTMTPF
                     LRGDDARRNRHKLYLGTLVAILAAVSLVAVKWVVMKMLPFDNKSEFQVVVDMPEGTAL
                     EETSRVLDLLAAEIARVPEVTDYQVYAGTSAPINFNGLVRQYYLRSGPELGDIQVNLV
                     DKHHRDRKSHEIAVAVRPALAEIGRRHGASVKVVEVPPGPPVQAPLVAEIYGPFVGMQ
                     REITRLVRKAFDAQPDIVDVDDSLESPRSRLVVAVDRQKAALLGVDQAQAVQVLAAAL
                     GGLDATYIRAGRETYPIPVRLELDVPEKARIADVLSLQVLSRSGARVPLSELTTVERR
                     PWDNAIYHKDLLPLTWVSGDAVGKVDSPLYGMFEVVGALKHAVPAGVELEQRFFSPPE
                     DPYRPTLKWDGEWQITFETFRDMGLAYSVGLVLIYLLVVAQFRSYAVPLIIMAPIPLT
                     IIGVMPGHALLGAQFTATSMIGMIALAGIIVRNSILLVDFINVEIAQGRSLADAVISA
                     AAVRARPIALTAVAAMTGAFFILDDPIFNGLAVSLIFGILVSTALTLVLIPILYFSYR
                     SRQQAA"
BASE COUNT          522 a         1165 c         1127 g          530 t
ORIGIN      
        1 tcacgctgcc gtcctcggca tgttccgcca cggtcatcac gccgtcctcg acgcccgtga
       61 tcttggcgtt ggtgatccag gtgatgtggc gctggcgcag ctccgactcc atcagcccct
      121 tgctgtcgcc gacgccgccg aggcccatgt ggccgatgta gggctcgggc gtcacgtaca
      181 ccatcggcac ccggtcgcgg atcttccggc ggcgcaggtc ggtatccagg atcatggccg
      241 actcgtacgc gggaccgaag cagctggcgc ccgcggcagc ccccacaacc acgggaccgg
      301 ggctctgcgc gaactgctgg taacgctgcc aggcctgctc cgaatgcccc tgcgtgcaga
      361 ccgactgcgt gaaccccttc ggcccgagtc ccggcacttc ctcgaaggcg agcttcggtc
      421 cggtcgtgat cacgaggtag tcataggcga gcgtcgagcc gtcctcgaac ttcagcgtcg
      481 atcccggcgc gtcgatcgcc tgcaccatct tcggcacgaa tgcgatgtcc tttgccgcga
      541 gcggcttcgc caggtcgacg cgcgtctgct cggccttgcg ccagccgacc atcatccacg
      601 ggttggacgg cgtgaactcg aagtacgggc tgctgccgac caccgtgacc tggtggccct
      661 tgccgacctt cttcttcatc tcgtaggcgc acgctacgcc gccgagacct gcacccaata
      721 ccacgatgtt cgccatggtc gcccccttgg ccgcgtcacg cggcctgctg tctgctgcgg
      781 tagctgaaat acaggatcgg gatcaggacc agcgtcagcg ccgtcgagac caggatcccg
      841 aagatcaggg aaactgcgag cccgttgaag atcgggtcgt cgaggatgaa gaacgcaccg
      901 gtcatggccg ccacggcggt gagcgcgatc ggacgcgcgc gcaccgccgc ggcgctgatc
      961 acggcatccg cgaggctgcg gccctgcgcg atctccacgt tgatgaagtc gacgagcagg
     1021 atcgagttgc gcacgatgat gccggcgagc gcgatcatgc cgatcatgga cgtcgccgtg
     1081 aactgggcgc cgagcagcgc gtggcccggc atcacgccga tgatggtgag cgggatcggc
     1141 gccatgatga tcagcgggac cgcgtaggag cggaactgcg cgaccaccag caggtagatg
     1201 aggaccaggc cgaccgagta ggcaagaccc atgtcgcgga acgtctcgaa cgtgatctgc
     1261 cactcgccgt cccacttgag cgtcgggcgg tagggatcct cgggcgggct gaagaaccgc
     1321 tgctccagtt ccacgccggc ggggaccgcg tgcttcagcg cccccaccac ctcgaacatc
     1381 ccgtacagcg ggctgtcgac cttgccgacg gcgtcgccgc tcacccacgt gagcggcagc
     1441 aggtccttgt ggtagatcgc gttgtcccac ggcctgcgct ccaccgtcgt gagttccgac
     1501 agcggcacgc gcgcgccgct gcgcgacagg acctgcagcg agagcacgtc ggcgatgcgg
     1561 gccttctccg ggacgtcgag ttccagccgg acgggtatcg gataggtctc gcggcccgcc
     1621 cggatgtagg tcgcgtcgag accgccgagg gccgccgcaa gcacctgcac cgcctgtgcc
     1681 tggtccacgc cgagcagggc cgccttctgc cggtcgaccg cgacgaccag gcgcgagcgc
     1741 ggcgactcga ggctgtcgtc cacgtcgacg atgtcgggct gggcgtcgaa cgccttgcgc
     1801 accagccgcg tgatctcgcg ctgcatgccg acgaacgggc cgtagatctc ggcgaccagc
     1861 ggcgcctgta cgggcggccc gggcgggacc tcgacgacct tcaccgatgc cccgtgacgg
     1921 cgccctatct ccgccagcgc gggacgcacc gcgacggcga tctcgtggct cttgcggtcg
     1981 cgatggtgct tgtccaccag gttgacctgg atgtcgccga gctccggccc gctgcgcagg
     2041 tagtactggc gcacgaggcc gttgaagttg atcggcgcgg acgtgcctgc gtagacctgg
     2101 taatcggtga cttccggcac cctggcgatc tcggcagcca acaggtcgag cacgcggctg
     2161 gtttcctcga gggccgtgcc ctcgggcatg tccaccacga cctggaactc cgacttgttg
     2221 tcgaagggca gcatcttcat gacgacccac ttcaccgcga ccagcgacac cgcggcgagg
     2281 atcgcgacca gcgtccccag gtacagcttg tggcggttgc gccgcgcgtc gtcgccgcgc
     2341 aggaatggcg tcatcgtccg cacgaagaac tggtgcagtc gcgcaccgcc gccgtgcgcg
     2401 gcgtgggcat gcgacagcat ccgcttcgag agccacgggg tgatgacgag cgcgatcgcg
     2461 agcgagatca gcatccccat gctcgcgttg atcgggatcg ggctcatgta gggacccatg
     2521 aggcccgaca cgaaggccat cggcagcagc gccgcgatca cggtgaaggt ggcgaggatg
     2581 gtcggtccgc cgacctcgtc cacggcccgc gggatgatcg tgcccatgcc cgcgtggccg
     2641 agtcccatgt gacggtggat gttctcgacg acgacgatcg cgtcgtcgac caggatgccg
     2701 atcgcgaaga tgagcgcgaa cagcgagacc cggttgatgg tgaatcccca cgcccaggac
     2761 gcgaacagcg tcgccgccag cgtgatcacg accgcggcgc cgacgaccac ggcctcgcgc
     2821 cagccgacgg tgagccagac gagcagcacc accgaggtgg tggcgaatgc cagcttctgg
     2881 atcagcttct gcgccttgtc gttggccgtg acgccgtagt cgcgcgtcac ggtgatctcg
     2941 acgccgtccg gtatgtacgt gccgcgcagc atctcgacgc gcttcagcac cgcgcgcgtc
     3001 acgtcgatgg cgttctcccc cggcttcttc gagatggcga tggtcacggc gggcgacgtg
     3061 ccggccggct ggatgccctt ctgcgcggcg gccggtccgg tgccgaacgt cacgtactgc
     3121 tccggctggt cgggcccttc ccgcaccgtc gccacgtcct gcaggtagac cgggcggccg
     3181 tcgtggacgg tgaccacgag ctgcgcgacc tggtcgcggt cggccaggaa ttcgccggcc
     3241 tgcaccggca cgtcccgccc gtccgccacg agcgaccccg cgtgctgcac gacgttcgcc
     3301 gcctgcagcg acgccgcgat ctcggtgacc gtgaggccgt agcc
//
LOCUS       DAOMFD010000214         3342 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_14006_length_3342_cov_31.340736, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000214 DAOMFD010000000
VERSION     DAOMFD010000214.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3342)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3342
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14006_length_3342_cov_31.340736"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2326
                     /locus_tag="PL152_09860"
     CDS             <1..2326
                     /locus_tag="PL152_09860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49633.1"
                     /translation="SLLHRLRERGVLRVAASYAVIAWLLLQVASVVLDPLGIPKWVMT
                     ALIIAAAVGFPVAVGLAWFLEIGEHGIEVDRAAEGVPRPTARGLRHYADVIVIGVLVI
                     AVVVLLVRQSDIGKPKPPSNPAIAVLPFENLSGDPAQEYFSDGLAEETLDRLGRVPGL
                     KVIARSSSFTFKGSKLDAKAIAEKLGVTTILEGSVRRDGRRLKLSARLIDGATGQQAW
                     SGSFDREMTDVFDVQAELAAAIVNAIVPAARGETVTTSAPPTTDLNAYDLYLLARAQL
                     VLRTPEGIQKSLDLSRQAVALDPSFARAHAHLATSILFYTMFFDPPQVERAANLRAAE
                     SAVHRALAIDPDLSEAHQAYAMLLRDTGQPGAEEEYRKALDLNPNNAAAWHDYAVFLG
                     NFPKREAESARATARSLELDPRQPSTWANYLGYVQRKEPARLPAELERAIRTVGDMPG
                     ALMRFPLVDWSSDREGIRRQFAHWWPRLKDGPGGADGIMLPDGALRGFPVEVLRSGLA
                     KENAPPVLGNMAPWLNRYRAWLTVDPVRALEALPGPGDRPRSGEHDAGLAEAPTIQKY
                     LEIDALGRTGNWKDLDRALAQLVGIRGADDRDALRIAAFWYAVQGRYEEAAGSLDKSL
                     PFPAQMFVPPVLGGDARQGMIEAAQVRILRGTGKSAEADRLARNLLDDLRRKRTSART
                     RCWAPDEDDTMRLAALAAGEGLKDEAVAALSDAMRCGDLPFGFEPELPWFKALEGYPP
                     YDELVRERTRRLAQARADMLALEAGAKRQAAILQ"
     gene            2379..3341
                     /locus_tag="PL152_09865"
     CDS             2379..3341
                     /locus_tag="PL152_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49634.1"
                     /translation="MRSITLAAAAALLATGSLAATAQDANFALPVPELEQMLANAPFT
                     IVAAEISRPKAKGDITLKADVSFDGKPPIRVKLRKAEPGADTFNNVPRYDLAAYELQK
                     LYLDPAEYVVPPTALRFVPLGDFRKYEPDTKRTFSGADEVLSVMQYWLQDIKVIADVY
                     DPAMFESDPVYARHIGQLNILTDLIDHGDSNVGNFLISKTTPGARVFSVDNGVAFASS
                     ESDRGRAWAELRVKRLPADAIDRLRKLTLDSMQQSLGVLVQWEMKDGAFHPVPPTANL
                     APSRGVRRSDAVLQMGLTQSEINKVWRNLQRLLAQVDSGNVTTF"
BASE COUNT          553 a         1160 c         1123 g          506 t
ORIGIN      
        1 cagcctgctg catcgcctgc gcgagcgcgg cgtgctgcgc gtggccgcga gctacgcggt
       61 gatcgcctgg ctcctgctgc aggttgcgtc ggtggtgctc gacccgctcg gaatcccgaa
      121 gtgggtcatg acggcgctga tcatcgccgc cgcggtcggc ttcccggtcg cggtcgggct
      181 cgcctggttt ctcgagatcg gcgagcacgg catcgaggtc gaccgcgccg ccgagggcgt
      241 gccccgcccg acggcgcgtg gcctgcgaca ctacgccgac gtcatcgtga tcggcgtgct
      301 cgtgatcgcg gtcgtcgtgc tgctggtgcg tcagtccgac atcggcaagc cgaagccgcc
      361 gtcgaacccg gcgatcgccg tgctgccgtt cgagaatctt tccggcgacc ccgcacagga
      421 atacttttcc gacggcctcg ccgaggaaac gctcgaccgc ctcggccgtg tcccgggtct
      481 caaggtcatc gcccgctcct cctcgttcac cttcaagggc agcaagctcg acgccaaggc
      541 catcgcggag aaactcggcg tgaccacgat cctggagggc agcgtgcgcc gcgacgggcg
      601 gcgcctcaag ttgagcgccc gcctcatcga cggcgccacc ggccagcaag cctggtccgg
      661 cagcttcgac cgcgagatga ccgacgtgtt cgacgtgcag gcagaactcg ccgcagcgat
      721 cgtcaatgca atcgtccccg ccgcgcgggg cgagaccgtg acgacgagtg cgccccccac
      781 gaccgacctc aatgcctacg acctctacct tcttgcacgc gcgcagctgg tgctgcggac
      841 gccggaggga atccagaagt ccctggacct gagccggcag gctgtcgcgc tcgatccctc
      901 attcgcacgc gcccatgcac accttgcgac gtccatcctg ttctacacga tgttcttcga
      961 cccgccgcag gtggaacgcg ctgcaaacct gcgcgcggcc gagtcagccg tgcaccgcgc
     1021 cctcgccatc gatcctgatc tctccgaggc tcaccaggcc tacgcgatgc tgctgcgcga
     1081 caccggccag ccgggcgccg aggaggaata ccgcaaggcg ctcgacctca atccgaacaa
     1141 cgctgcagcc tggcacgact atgccgtctt cctggggaac ttcccgaagc gcgaagcgga
     1201 atcggccagg gcaacggcgc gcagcctgga actcgatccg cgccagccgt cgacatgggc
     1261 caattatctc ggctacgtgc agcgcaagga gccggcgcgg ttgccggcgg aactcgagcg
     1321 tgcaatccgg acggtcggcg acatgcccgg tgcgctcatg cgctttccgc tcgtcgactg
     1381 gtccagcgac cgcgagggaa ttcgccggca attcgcacac tggtggccgc gcctcaagga
     1441 cgggccgggc ggggccgacg gaatcatgtt gccggacggc gccctgcgcg gctttccggt
     1501 cgaggtgctt cgatccggtc tcgcgaagga gaatgcaccg cccgtccttg gcaacatggc
     1561 accgtggctc aatcgctacc gggcgtggct gaccgtggac ccggttcgcg ccctcgaggc
     1621 gctgcccggc ccgggtgatc ggccgcggag cggtgagcac gacgctggtc ttgccgaggc
     1681 cccgacgata caaaagtacc tggagatcga cgctctgggc cgcaccggga actggaagga
     1741 cctcgatcgc gccctcgcgc aactggtcgg gatccgggga gccgacgatc gcgatgcgct
     1801 tcggatagcg gctttctggt acgccgtgca gggccgctat gaggaagcgg ccgggtccct
     1861 cgacaagtcg ctgccgtttc ccgcacagat gttcgtgccc ccggtcctgg gcggcgacgc
     1921 gcgacagggc atgatcgagg ccgcacaggt ccgcatcctg cgcgggaccg gcaagtcggc
     1981 ggaggccgac aggctcgccc ggaacctcct cgacgacttg cgcaggaaac gcacgtccgc
     2041 aaggacacgc tgctgggcgc ccgacgagga cgacacgatg cggctggctg ccctcgcggc
     2101 cggtgagggc ctgaaggacg aagccgtcgc ggcgctgagc gacgccatgc gctgcggcga
     2161 tcttccgttc gggttcgagc cggagctgcc gtggttcaag gccctggaag gctatccgcc
     2221 gtacgacgag ctggtccgcg aacggacgag gcgcctcgcg caggcccggg cggacatgct
     2281 ggcgctcgag gccggagcca agcggcaggc ggcgatactg cagtaagctt gaacgacggg
     2341 cgccgcatgg gagtcgctcg cggtcacgga ggtgcgcaat gcgatcgatc accctagccg
     2401 ctgccgcggc cctgctggcg accggcagcc tcgcggcgac tgcgcaggat gcgaacttcg
     2461 cactgccggt tcccgagctc gagcagatgc tggcgaacgc cccgttcacc atcgtggccg
     2521 ccgagatcag ccggccgaag gccaagggcg acatcacgct caaggccgac gtctcgttcg
     2581 acggcaagcc gccgatccgc gtcaagctgc gcaaggccga gcccggcgcc gacacgttca
     2641 acaacgtgcc gcgctacgac ctggccgcct acgaactgca gaagctgtac ctggatccgg
     2701 cggagtatgt ggtcccccct accgcgctgc gcttcgtgcc cctgggcgac ttccgcaagt
     2761 acgagcccga cacgaagcgg acgttctccg gcgccgacga ggtcctgagc gtcatgcagt
     2821 actggctgca ggacatcaag gtgatcgccg acgtgtacga cccggccatg ttcgagtcgg
     2881 atcccgtcta tgcgcgacac atcggccagc tcaacatcct caccgacctg atcgaccacg
     2941 gcgactcgaa cgtggggaac ttcctgattt cgaagacgac gccgggggca cgcgtatttt
     3001 cagtggacaa cggggtggct ttcgcatcca gcgagagtga ccgcggccgc gcatgggccg
     3061 aactgcgcgt caagcgcctg ccggccgacg cgatcgaccg gctgcgcaag ctgaccttgg
     3121 actccatgca gcaatcgctc ggcgtcctgg tgcaatggga gatgaaggac ggcgcgttcc
     3181 accccgtccc gccgacggcc aacctcgcgc cgtcgcgcgg tgtgcggcgc agcgatgcgg
     3241 tcctgcagat gggactgacg cagtcggaga tcaacaaggt ctggcgcaac ctgcaacgcc
     3301 tgctggcaca ggtggacagc gggaacgtga cgacgttctg ag
//
LOCUS       DAOMFD010000215         3303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_14268_length_3303_cov_31.090825, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000215 DAOMFD010000000
VERSION     DAOMFD010000215.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14268_length_3303_cov_31.090825"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1410
                     /gene="coaBC"
                     /locus_tag="PL152_09870"
     CDS             94..1410
                     /gene="coaBC"
                     /locus_tag="PL152_09870"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HQR49635.1"
                     /translation="MKPSQPRRILLGVTGGIAAYKSADLVRRLRERGADVQVVMTAGA
                     RQFVTPLTFQALSGRPVRSDLWDEAAEAAMGHIELARWADQILVAPATADFIARLANG
                     LADDLLSTLCLATDAPIAIAPAMNRLMWANAATRTNVDTLRARGIGILGPAEGDQACG
                     ETGPGRMLEPADLADAVLRDAPTAERQSATPVIAGSPAAPSALGLLAGRKVVITAGPT
                     RERIDPVRFITNRSSGKMGYAVAEAARDAGAEVVLVSGPVNLPAPAGIRRVCVETAEQ
                     MLGAVQAEIAGTDVFIAAAAVSDYRPLQVSAEKIKKTSDSLLLALSRTPDILATVAAG
                     SPRPFVVGFAAETQNVERNALAKLEGKRLDMIAANQVGDGLAFDCDDNALTVYWPDGK
                     RELPRSGKPELAARLIAVVAERFAAKRGQGVAIAAEPRAPGSAAAR"
     gene            1407..1880
                     /gene="dut"
                     /locus_tag="PL152_09875"
     CDS             1407..1880
                     /gene="dut"
                     /locus_tag="PL152_09875"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HQR49636.1"
                     /translation="MSGTTGARRLQVRILDPRIGREIPLPHYATSGSAGLDLRACIDA
                     PLVLDPAAATLVPTGIAIHLDDPGLAAVLLPRSGLGHKHGIVLGNLVGLIDSDYQGQV
                     MVSCWNRGLTAYTIQPGERIAQMVVVPVVQVEFDVVENFTESHRGAGGFGSSGTT"
     gene            complement(1873..2547)
                     /locus_tag="PL152_09880"
     CDS             complement(1873..2547)
                     /locus_tag="PL152_09880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49637.1"
                     /translation="MHRHTRLTTILAGPALLLLAGLAALPGESLAAKPTRTAKPTGHE
                     SVYRCKDPEGHTYVGQNIPEQCMNVDVEVLDDTGRVVRIIPGPASQQEVEDKKAAEAA
                     RIAAANRDKTLLATYLTVADIERLRDQRLELLVQQANVTEQYVENLKEREARLAQDVR
                     RFRPYSDKPGAPALPDHIAEDIVNTVNGLQVYQQELAKNTAEQQRLRTEFASDIARFK
                     ELKGLK"
     gene            complement(2630..3262)
                     /gene="pyrE"
                     /locus_tag="PL152_09885"
     CDS             complement(2630..3262)
                     /gene="pyrE"
                     /locus_tag="PL152_09885"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HQR49638.1"
                     /translation="MHEPDFIRLCLDLGVLRFGEFTLKSGRVSPYFFNAGLFNNGRAI
                     AALGRHYARAAVLSGVPFDMLFGPAYKGIPLVTTTAAALAEHEGTNLPFAFNRKEAKD
                     HGEGGTIVGAPLAGRVLIVDDVITAGTAIRESVEIIRAHGATCAGVLIALDREERGQG
                     ERSAVQEVQVEFGLPVVSVLRLRDLMVHLEETGNAAVLASVRAYRDRYGV"
BASE COUNT          473 a         1153 c         1145 g          532 t
ORIGIN      
        1 tttcctaacg gccggttatg acctgagacc gaagcgtcgc agcgtggcgc ggtcctttgc
       61 gggccggctg ccgcccgtcc cataatgcgt cccatgaagc cgtcacagcc gcggcgcatc
      121 ctgctcgggg tcaccggcgg aatcgccgcg tacaagagcg ccgatctcgt gcgccgcctg
      181 cgcgagcgcg gcgcggacgt gcaggtcgtg atgacggccg gcgcgcgcca gttcgtcacg
      241 ccgctcacgt tccaggcgtt gtccgggcgg cccgtgcgct ccgacctgtg ggacgaagcg
      301 gccgaggcgg cgatgggtca catcgagctc gcgcgctggg cggaccagat cctcgtggct
      361 cctgccacgg cggatttcat cgcgcggctg gcgaacggcc tcgccgatga tctgctcagc
      421 accctgtgcc tcgccaccga cgcgccgatc gcgatcgccc cggcgatgaa tcgcctcatg
      481 tgggcgaacg ccgcgacgcg caccaacgtc gacacgctgc gcgcccgcgg catcgggatc
      541 ctcgggccgg cggagggtga ccaggcctgc ggagagaccg ggccgggacg catgctcgag
      601 cccgccgacc tcgccgatgc agtgctgcgc gatgccccga ctgctgaacg gcaatcggcg
      661 accccggtga ttgccgggtc tccagcggcg ccgtctgcac ttggcctgct cgcgggccgc
      721 aaggtcgtca tcaccgcggg cccgacgcgt gaacgcatcg accccgtccg gttcatcacc
      781 aatcgcagct ccgggaaaat gggttacgcg gtcgcggagg cagcacgtga tgccggtgcc
      841 gaggtcgtgc tcgtcagcgg gccggtcaat ctcccggcgc ccgcgggcat tcgtcgcgta
      901 tgcgtcgaga cagcggagca gatgctcggc gcggtgcagg ccgaaatcgc cggcaccgac
      961 gtcttcatcg ccgcggccgc ggtgtccgat taccggcctc tgcaggtgtc ggccgagaag
     1021 atcaagaaga ccagcgactc gctgctgctc gccttgtcgc gcacgccgga catcctcgcg
     1081 accgtggccg cgggcagccc gcgcccgttc gtcgtggggt tcgcggccga gacccagaac
     1141 gtcgagcgca atgcgctcgc caagctcgag ggcaagcgcc tcgacatgat cgccgcgaac
     1201 caggtcggcg atggcctcgc gttcgactgc gacgacaatg cgctcaccgt gtactggccc
     1261 gacggcaagc gcgaattgcc gcgctcgggc aagccggagc tcgccgcgcg gctgatcgcg
     1321 gtggtggccg agcgcttcgc ggcgaagcgg ggccagggcg tcgccatcgc cgcggagccg
     1381 cgcgcgccag gcagcgcggc cgcacgatga gtggcacgac cggggcgcgc cggctgcagg
     1441 tacggatcct cgatccacgc atcggcaggg aaatcccgct accgcattac gcgacgtctg
     1501 ggtcggctgg cctcgacctg cgcgcctgca tcgacgcgcc gctggtgctc gatccagccg
     1561 ctgccaccct ggtacccacg ggaatagcca tccacctcga cgacccggga ctcgccgccg
     1621 tgctgctgcc gcgatcgggg ctggggcaca agcacggcat cgtgctcggc aacctcgtcg
     1681 gcctgatcga ctcggactac caggggcagg tcatggtgtc ctgctggaac cgcggactga
     1741 cggcgtacac gatccagcct ggcgagcgca tcgcccagat ggtggtcgtg ccggtcgtgc
     1801 aggtggaatt cgacgtcgtc gagaatttca ccgagagcca ccgcggcgcc gggggcttcg
     1861 ggagttcggg gactacttga gccccttcag ttccttgaag cgcgcgatgt cggacgcgaa
     1921 ttccgtgcgc agccgctgct gctccgccgt gttcttggcg agttcctgct ggtagacctg
     1981 caggccgttc accgtgttca cgatgtcttc cgcgatgtgg tcgggcagcg cgggcgcgcc
     2041 cggcttgtcg ctgtagggcc ggaagcgccg tacgtcctgc gccagccggg cctcgcgctc
     2101 cttcaggttc tcgacgtact gctcggtgac attggcctgc tggaccagca gttcgagccg
     2161 ctggtcacgc agtcgttcga tgtcggcgac cgtcaggtag gtcgcgagca gcgtcttgtc
     2221 gcgatttgcg gccgcgatgc gcgcagcctc cgcggccttc ttgtcctcga cttcctgctg
     2281 ggacgcgggg ccgggaatga tgcgcacgac gcggcccgta tcgtcgagga cctcgacgtc
     2341 cacgttcatg cactgctcgg ggatgttctg gccgacgtag gtgtggccct ccgggtcctt
     2401 gcagcggtac acgctctcgt gcccggtcgg cttggcggtc ctggtcggct tggcagccag
     2461 ggattcgccc ggcaaggcag cgagccctgc gagcaggagc agggccggtc cggccaggat
     2521 cgtcgtgagt ctcgtgtgcc gatgcatggg agcggattct gcccctcggt cgctgccggg
     2581 cctatcttgg cccatccgta ccgtgaacta tgtcaaacgc cgcggcgaat tacacgccat
     2641 accggtcccg gtaggcgcgc acggacgcga ggaccgcggc gttgccggtc tcctcgaggt
     2701 gcaccatcag gtcccggagt cgcagcacgc tgacgaccgg caggccgaac tcaacctgga
     2761 cctcctgcac ggccgaacgc tccccctgcc cccgctcctc acggtcgagg gcgatcagca
     2821 cgcccgcgca ggtcgcgccg tgcgcgcgga tgatctcgac cgactcgcgg atggccgtgc
     2881 ccgcggtgat cacgtcgtcc acgatgagga cgcggccggc gagcggcgcg ccgacgatcg
     2941 tcccgccttc cccatggtcc ttcgcctcct tgcggttgaa ggcgaacggc aggttcgtcc
     3001 cttcgtgctc cgcgagcgcc gcggcggtcg tcgtcacgag cgggatcccc ttgtaggccg
     3061 gcccgaacag catgtcgaac ggaacgcccg acagcactgc cgcgcgggcg tagtggcggc
     3121 cgagcgccgc gatcgcgcgg ccgttgttga acagccctgc gttgaagaag tacgggctca
     3181 cgcgcccgga cttgagcgtg aactcgccga agcgcagcac gccgaggtcg aggcacaggc
     3241 gaatgaagtc gggctcgtgc atggcgctac tttatactgc gcggccatgc gcatcgtcac
     3301 cct
//
LOCUS       DAOMFD010000216         3221 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_14916_length_3221_cov_40.550537, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000216 DAOMFD010000000
VERSION     DAOMFD010000216.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3221)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14916_length_3221_cov_40.550537"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            142..2022
                     /locus_tag="PL152_09890"
     CDS             142..2022
                     /locus_tag="PL152_09890"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HQR49639.1"
                     /translation="MSQAAQQAPDVDALVRASVARWRHEPTRLLQVLREVQEACNYIP
                     PPALTLIAHLLRLPLSRVKGVAGFYSFLSTEPWGRYRVLFSDNVTDRMHGSERLLAEM
                     CHRLWVEAGKVSEDGLVSVATTSCTGLCDQGPGMLVNGRAIGRLTSERVEQICGLILR
                     GVPLDEWPAALFHVDDNVRRADIMLGHRLQPGDAVRAALRRGVQGHVEHAANARSWRE
                     GFTGSANGPVAVLEEVKRSNLRGRGGAGFTTGIKWEACRHATGNARYVICNADEGEPG
                     TFKDRVLLTSHADLLFDGMTVAAYAVGASQGFVYLRGEYRFLLDRLNETLERRRRDHL
                     LGNAICGQPGFDFDIEIHVGAGSYVCGEESALIESLEGKRGVPRNRPPFPVTSGYLQQ
                     PTIVNNVETLCAAALIAVRGGEWYRSIGTLKSSGTKILSVAGDCDRPGLYEYPFGVTL
                     RQVLEECGATRTQAVQVSGPSGTCVDAGEFDRRIAFEDLPTAGAFTVFDTSRDMFEIA
                     RAYAQFFAHESCGFCTPCRAGTSMLMHSMDKIRDGNGTLHDLREIARLNKVLLMSAHC
                     GLGHTACNPILDTMKKFRPVYERRLRSRDFAPAFDLDEALARARQMTGRDDPGAHLEN
                     LP"
     gene            2019..2756
                     /locus_tag="PL152_09895"
     CDS             2019..2756
                     /locus_tag="PL152_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQR49640.1"
                     /translation="MNDRPTFLLDGEEVPFTPGQTVLQAALAAGRYIPHLCYHPEFTP
                     HGSCKVCTAKVNGRTVASCTTPAESAIEVESNTDELNDLRRTLVQMLFAEGNHFCPSC
                     EKSGNCVLQALGYDLGVMTAGFRHLYPDRPVDASHPELLLDFNRCVLCELCVRASRDV
                     DGKSVFALSGRGLTKHLIVNSESGLLRDTDVAVTDKAVQVCPVGVILRKRVGFAVPVG
                     QRRYDQRPISAQALDDAPRPPSGVSHD"
     gene            2749..>3221
                     /locus_tag="PL152_09900"
     CDS             2749..>3221
                     /locus_tag="PL152_09900"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP oxidoreductase"
                     /protein_id="HQR49641.1"
                     /translation="MTEPLPMATATAGKPKLRVATTSLAGCFGCHMSFLDIDERLFEL
                     LEHVEFDRSPLTDIKHCGPCDIGIVEGGLCNAENVQVLREFRANCKVLVAIGACAVTG
                     GLPAQRNHLDLGQCLQEVYVTEPSVGLGRVPNDPELPLPLDKVHPLHEVVKVDYF"
BASE COUNT          510 a         1128 c         1099 g          484 t
ORIGIN      
        1 aatccctggt tcacgcaaga ggtcattcct caacttcgtg aacggattca cgcgcttacg
       61 ggagggtttt ccgagattgc ccgcctgccg cagccggcgc agtctgcacg gcgggaacag
      121 gatcggtagg cggcaaggac catgtcacaa gcggcgcagc aggcaccgga cgtcgacgcg
      181 ttggtgcgcg cctcggtcgc gcgctggcgg cacgagccca cgcggctgct gcaggtgctg
      241 cgcgaggtgc aggaagcctg caactacatc ccgccgcccg cgctgacgct catcgcgcac
      301 ctgctgcgcc tgccgctctc ccgcgtgaag ggcgtcgccg gcttctattc cttcctctcc
      361 accgagccct ggggccggta ccgcgtgctc ttttcggaca acgtcaccga ccgcatgcac
      421 ggcagcgaac ggctgctggc cgaaatgtgc catcgcctgt gggtcgaggc cggcaaggtg
      481 tccgaggacg ggctggtgag cgtggcgacg acctcgtgca cgggcctctg cgaccaggga
      541 ccgggcatgc tggtgaatgg ccgtgcgatc gggcggctca ccagcgagcg cgtcgagcag
      601 atctgcggcc tcatcctgcg cggagtgcca ctcgacgagt ggccggctgc gctgttccac
      661 gtcgatgaca acgtgcgccg tgccgacatc atgctcggcc accgacttca accgggcgat
      721 gcggtccgcg ccgcgctgcg acgcggcgtg caggggcacg tcgagcacgc ggccaacgcc
      781 cgctcgtggc gcgagggatt caccggctcg gcgaacgggc cggtcgccgt gctcgaggag
      841 gtgaagcgct cgaacctgcg cggccgcggc ggcgcgggat tcacgaccgg catcaagtgg
      901 gaagcgtgcc ggcacgccac cggcaacgcg cgctacgtga tctgcaatgc cgacgaaggc
      961 gagcccggca cgttcaagga ccgcgtgctg ctcacgagcc acgcggacct gctcttcgac
     1021 ggcatgacgg tggccgccta cgcggtgggc gcgagccagg ggttcgtgta cttgagaggg
     1081 gaataccgct tcctcctgga ccgactcaac gagaccctcg agcgccgtcg ccgggatcac
     1141 ctgctcggca acgcgatctg cggccagccg ggattcgact tcgacatcga gatccacgtc
     1201 ggcgcgggtt cgtacgtctg tggcgaggag tccgcgctca tcgagtcgct ggaaggcaag
     1261 cgcggcgtgc cgcgcaaccg gccgccgttc cccgtgacca gcggctacct gcagcagccg
     1321 accatcgtca acaacgtcga gaccctgtgc gcggcggcgc tcatcgcggt gcgcggcggc
     1381 gagtggtatc gctcgatcgg cacactcaag tcgtcgggca ccaagatcct gtccgtggcg
     1441 ggcgactgcg accgccccgg cctctacgaa tacccgttcg gcgtgacgct gcgacaggtg
     1501 ctcgaggagt gcggcgccac gcgcacacag gcggtgcagg tgagcggccc gtccggcacg
     1561 tgcgtggacg ccggggagtt cgaccggcgc atcgcgttcg aggacctgcc gaccgcgggc
     1621 gcgttcacgg tgttcgacac gagccgcgac atgttcgaga tcgcgcgcgc ctacgcgcag
     1681 ttcttcgccc acgagagctg cggattctgc acgccgtgcc gcgccggcac ctccatgctg
     1741 atgcactcga tggacaagat ccgcgacggc aacggcacgc tgcacgacct gcgcgagatc
     1801 gcgcgactga acaaggtgct gctcatgtcg gcgcactgcg gcctcgggca cacggcctgc
     1861 aacccgatcc tcgacaccat gaagaaattc cgcccggtgt acgagcggcg gctgcgctcg
     1921 cgcgacttcg cgccggcctt cgacctcgac gaggcgctcg ctcgcgcccg ccagatgact
     1981 ggtcgcgacg atcccggcgc gcacctggag aacctgccgt gaacgaccgg ccgacgttcc
     2041 tgctcgacgg cgaggaggtc ccattcacgc cggggcagac ggtcctgcag gcggcgctcg
     2101 ctgccggccg ctacatcccg cacctgtgct atcacccgga gttcaccccg cacggcagct
     2161 gcaaggtctg cacggccaag gtgaacggcc gcacggtcgc ctcgtgcacc acacccgcgg
     2221 aatcggcgat cgaggtcgag agcaacaccg acgagctgaa cgacctgcgc cgcacgctgg
     2281 tgcagatgct gttcgcggaa ggcaaccact tctgcccctc ctgcgagaag agcggcaact
     2341 gcgtgctgca ggcgctcggc tacgacctcg gcgtcatgac cgccggattc cggcacctgt
     2401 accccgaccg gcccgtggat gcctcgcacc cggagctgct gctcgacttc aaccgttgcg
     2461 tcctctgcga gctgtgcgtg cgggcctcgc gcgacgtgga cggcaagagc gtgttcgcgc
     2521 tgtccggccg tgggctcacc aagcacctca tcgtcaactc ggaaagcggc ctgctgcggg
     2581 acaccgacgt cgcggtcacc gacaaggcgg tgcaggtctg cccggtgggc gtgatcctgc
     2641 gcaagcgggt tggcttcgcc gtgcccgtcg gccagcgccg ctacgaccag cggccgatca
     2701 gcgcgcaggc gctggacgat gcgccgcggc cgccgagcgg ggtcagccat gactgagccg
     2761 ctgcccatgg cgaccgcgac cgccgggaag cccaagctgc gcgtggcgac cacctcgctc
     2821 gccggctgct tcggctgcca catgtcgttc ctcgacatcg acgagcgtct cttcgaactg
     2881 ctcgagcacg tggagttcga ccggtcgccg ttgaccgaca tcaaacactg cggaccctgc
     2941 gacatcggca tcgtcgaggg cgggctgtgc aacgccgaga acgtgcaggt gctgcgcgag
     3001 ttccgcgcga actgcaaggt gctcgtcgcg atcggtgcct gcgcagtgac gggcgggctg
     3061 ccggcgcaac gcaatcacct ggacctcggc cagtgcctgc aggaggtgta cgtcaccgaa
     3121 cccagcgtcg ggctcggtcg cgtcccgaac gacccggagc tgccgctgcc gctcgacaag
     3181 gtgcacccgc tgcacgaggt cgtgaaggtg gactacttca t
//
LOCUS       DAOMFD010000217         3220 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_14922_length_3220_cov_28.872986, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000217 DAOMFD010000000
VERSION     DAOMFD010000217.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3220
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14922_length_3220_cov_28.872986"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1158)
                     /gene="icd"
                     /locus_tag="PL152_09905"
     CDS             complement(<1..1158)
                     /gene="icd"
                     /locus_tag="PL152_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005158038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004450 - isocitrate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HQR49642.1"
                     /translation="MSSQLKLNVPAEGQKITISNGRLQVPDQPIVPFIEGDGTGPDIW
                     RASVRVLDAAVAKSYGGKRRIHWMEVYAGQKSNDLYNTWLPDETVQACRDYLVSIKGP
                     LTTPIGGGIRSLNVALRQMLDLYVCLRPVRWFKGVPSPVKAPEKVDMVIFRENTEDIY
                     AGIEFEAGSADGEKFKQLFKDAFPKLYGKIRFPDSSGIGIKAVSREGTERLVRAAIQY
                     AIDNKRKSMTIVHKGNIMKFTEGAFRNWGYALAEREFAQHTYTWDQWERTRADKGEKA
                     ANAEQDAAKKAGKVIVKDAIADITLQQVLTRPDEFDVIATMNLNGDYLSDALAAQVGG
                     IGIAPGGNINYMTGHAVFEATHGTAPKYANLDKVNPGSVILSGEMMLRYMGW"
     gene            complement(1249..2508)
                     /locus_tag="PL152_09910"
     CDS             complement(1249..2508)
                     /locus_tag="PL152_09910"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HQR49643.1"
                     /translation="MRRHPFGLLPAALAVAATVSCGTGDRSHSEWLGPPRSLVTPAGP
                     GSQFPRLTSSRGQNPVMSWLEPAAGEMSLRYSQWSGRGWGAARVVAQGADWFVNWADF
                     PSVVAADAGHRGAHWLQQVPGNVYSYVVRIATSGDGGRTWSAPITPHDDGTPTEHGFV
                     TLLPYEGQLLAVWLDGRRTVGNHDHDHGDGHDHPEAASEGAMTLRSAMVGRDGVQLGP
                     DVELDSRTCDCCQTGAAVAASGPIVVYRDRGEDGTRDISLVRFDGNRWSTPAPVARDG
                     WNIDACPVNGPAVDALGDTVVVAWFTGAGGPRVRLAFSTDGGRSFGTPMDVDGGRVSG
                     RVDVVLLDDGRAVVSWLRESAGRDALAARPYTQDGPVGRTVEIAHADRARAAGFPQVA
                     RAGDALLFAWTEDGATPQVRTVVAPLR"
     gene            2613..3023
                     /locus_tag="PL152_09915"
     CDS             2613..3023
                     /locus_tag="PL152_09915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49644.1"
                     /translation="MGTTRLDRGIDWMRGRQRSLVNAVLVLFCLAWLQAAALPCAMAT
                     PTTAAPDGGQHCPYCPQTGSGPAATDHSGSCAYPHQPQADARDAGIVFIALPATRSAD
                     TLQPVLTEGAAPFATERDPAPPVPLPVQYCRFLK"
BASE COUNT          459 a         1139 c         1084 g          538 t
ORIGIN      
        1 ccagcccatg tagcgcagca tcatctcgcc ggagaggatc accgagcccg ggttgacctt
       61 gtcgaggttc gcgtacttgg gggccgtgcc gtgcgtggct tcgaacaccg cgtgaccggt
      121 catgtagttg atgttgccgc ccggggcgat gccgatgccg ccaacctgtg ccgccaatgc
      181 gtcggagagg taatcgccgt tcaggttcat cgtggcgatc acgtcgaact cgtcggggcg
      241 ggtcagcacc tgctgcagcg tgatgtccgc gatcgcgtcc ttgacgatga ccttgccggc
      301 cttcttcgcg gcgtcctgct cggcattggc ggccttctcg cccttgtcgg cgcgggtgcg
      361 ctcccactgg tcccaggtgt acgtgtgctg cgcgaactcg cgctccgcga gcgcatagcc
      421 ccagttgcgg aacgcaccct ccgtgaactt catgatgttg cccttgtgca cgatcgtcat
      481 gctcttgcgc ttgttgtcga tcgcgtactg gatggcggcg cgcaccaggc gctcggtgcc
      541 ctcgcgggac acggccttga tgccgatgcc cgagctgtcc gggaagcgga tcttgccgta
      601 gagcttcggg aacgcgtcct tgaagagctg cttgaacttc tcgccgtcgg cgctgccggc
      661 ctcgaactcg atgcctgcgt agatgtcctc ggtgttctcg cggaagatca ccatgtccac
      721 tttttccggc gccttgacgg gcgagggcac gcccttgaac cagcgcaccg ggcgcaggca
      781 cacgtacagg tccagcatct gccgcaacgc cacgttgagc gagcggatgc cgccgccgat
      841 gggggtcgtg agcggaccct tgatggacac gaggtagtct cggcaagcct gcacggtttc
      901 gtccggcagc caggtgttgt agaggtcgtt ggacttctgc ccggcgtaga cctccatcca
      961 gtggatcctg cgcttgccgc cgtaggactt ggcgaccgcg gcgtcgagca cgcggaccga
     1021 cgcgcgccag atgtctgggc cggtgccgtc accttcgatg aacggcacga tcggctggtc
     1081 gggaacctgc agccggccgt tcgaaatcgt gatcttctgg ccctccgcgg gcacgttcag
     1141 cttgagctgg ctggacatct gtcgagctcc ggaaatgcgc ggtgtgacgg aaattcgagg
     1201 ggtgaaatct tagcacggca gccgccggcg cgggccgcgc cggccgtgtc agcgcagcgg
     1261 tgccacgacc gttcgcacct gcggtgtcgc gccgtcttcg gtccaggcga acaggagagc
     1321 gtcaccggcg cgcgcgacct gcgggaagcc cgcggctcgt gcgcggtccg cgtgcgcgat
     1381 ctcgaccgtg cgcccgaccg gaccgtcctg cgtatacggc cgggccgcga gggcgtcgcg
     1441 acccgccgac tcgcgcagcc agctcaccac cgcgcgcccg tcgtcaagga gcacgacgtc
     1501 gacgcggccg ctgacccgcc cgccgtccac gtccatcggc gtgccgaagc tgcgcccgcc
     1561 gtccgtcgag aaggccaggc gcacgcgcgg cccgcccgcg cccgtgaacc aggcgaccac
     1621 gaccgtgtcg ccgagcgcgt ccacggccgg tccgttcacc gggcaggcat cgatgttcca
     1681 gccgtcgcgc gccacgggtg cgggcgtcga ccatcgattg ccgtcgaacc gcacgagcga
     1741 gatgtcgcgc gtcccgtctt cgccgcgatc gcggtaaacg acgatcggcc ccgaagcagc
     1801 gacggcggcc ccggtctggc agcaatcgca ggtgcggctg tcgagttcca cgtccggccc
     1861 gagctgcacg ccgtcacggc cgaccatcgc actgcgcagg gtcatcgcgc cctcgctcgc
     1921 ggcctcggga tggtcatgac catcgccatg gtcgtgatca tggttgccga ccgtgcgtcg
     1981 cccgtccagc cagacggcca gcaactggcc ttcgtacggc agcagggtca cgaaaccgtg
     2041 ttcggtgggc gtgccgtcgt cgtgcggcgt gatcggcgcg gaccacgttc ggccgccgtc
     2101 gccactggtc gcaatgcgca cgacatagga gtagacattg ccaggcacct gttgcagcca
     2161 gtgcgcgccc cggtggccgg cgtcggccgc gacgaccgac ggaaagtcgg cccagttcac
     2221 gaaccagtcg gcgccttgcg cgacgactcg cgccgcaccc catccccggc cgctccactg
     2281 ggaatagcgc agcgacatct ctcccgccgc tggttcgagc cagctcatga cggggttttg
     2341 accgcgggac gatgtcaggc gagggaactg gctgcccggg cccgccggcg tcaccagcga
     2401 gcgcggcggc ccgagccact ccgagtggct gcgatcgccg gtgccgcacg acacagtcgc
     2461 ggcgaccgcc agcgctgccg gcaacaagcc gaacgggtgc cgtcgcatgc cgccgattgt
     2521 cgtcgattcc tagtggtttg ccgcaggttt cggcactgcg gacgcatgat tccttgccag
     2581 ccgccgtcga tcgcgctagc ttcgggcccg ccatgggcac gacgagactc gatcgcggga
     2641 tcgactggat gagggggcgg cagcgctcgc tggtaaacgc cgtgctcgtg ctgttctgcc
     2701 ttgcgtggct gcaggcagcg gcattgccgt gcgccatggc tacgccgacg acggccgccc
     2761 cggacggcgg ccagcactgc ccgtactgcc cgcagaccgg ctccggaccg gccgcgacgg
     2821 atcactcggg ctcgtgcgcc tacccccacc aaccgcaggc cgacgcgcgc gatgccggca
     2881 tcgtgttcat cgcgcttcct gcgacgcgca gcgccgacac gctgcagcct gtgctgaccg
     2941 agggcgcagc gcccttcgcc acggaacggg atcctgcgcc gcccgtcccg cttccagtcc
     3001 aatactgcag gtttctcaag tagccttgct cccggctccc gtccgtccag gacgcgttcc
     3061 ccagtgttct tgcccgggag gctctcatgg cacaggtacg cccggcgcct gcacaaggcc
     3121 ggttgatcag gaatcgaagg tccgcgccga ccgtcacggc gctcgtagcc ttgctgtcgg
     3181 cggggccgac ggtgcacgcg gatccgacgc tgtcgctggc
//
LOCUS       DAOMFD010000218         3184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_15219_length_3184_cov_39.449025, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000218 DAOMFD010000000
VERSION     DAOMFD010000218.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15219_length_3184_cov_39.449025"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PL152_09920"
     CDS             <1..362
                     /locus_tag="PL152_09920"
                     /inference="COORDINATES: protein motif:HMM:NF036806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HQR49645.1"
                     /translation="SIHSPLSRGCHRLIRQGAKLVETADDIYAELGALVAGLGSLPAA
                     EATEPQQDSGLSLDKDYEILLDALGFEPAGVDTLVARTGLEAGAVASMLLILELEGRI
                     APQPGGLYCRRQPGKKA"
     gene            359..838
                     /locus_tag="PL152_09925"
     CDS             359..838
                     /locus_tag="PL152_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HQR49646.1"
                     /translation="MKEGVLDILIYLFENYFDGELDEGEEPDRETLKQELEHAGFPTG
                     EVERALCWLEDLAADPSRTGPAPTARAIRVFDALEQARLDTDCRGFLVHLEQVGILSP
                     TQRELVIERLMALDGDDIDIERLKWVVLMVLFSQPGQETAFSRMEDLVFEEHSGAVH"
     gene            924..>3184
                     /locus_tag="PL152_09930"
     CDS             924..>3184
                     /locus_tag="PL152_09930"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase I"
                     /protein_id="HQR49647.1"
                     /translation="MSRNLVIVESPAKAKTIKKYLGKDFEVMASYGHVRDLVPKEGAV
                     DPARRFAMRWELIDKNRKHVDAIARAVKKADALYLSTDPDREGEAISWHLYELLKDAG
                     VLDGKAVHRSVFYEITQGAVQDAIGRPREIAEELVKAYLARRALDYLVGFNISPLLWK
                     KAGLSGASAGRVQSPALRMICEREEEIRLFKPREYWTVDAEAEKSSQVFNARLTEYGG
                     RKVEQFTITSAAQADEVRRTLEQSAGGALAVTAVERKQRKRNPSPPFTTSTLQQEAAR
                     KLGFGAQRTMRTAQKLYEGVDFGEGPVGLITYMRTDSVNLGVEAVREIRGVIGRLYGA
                     EALPESPRVYRTKSKNAQEAHEAIRPTAAARTPEQLAGRLDPDQHKLYTLVWRRAVAS
                     QMEAAVFDTVAVDLTAGSGHTLRANGSTLVTPGFLAVYRESFDDATLDEDDERRLPPL
                     ETGDRVMLNAISPAQHFTEPPPRYSEASLVKALEEYGIGRPSTYASIIQTLLYKKYCE
                     LTNRRFVPTDLGKIVNRFLTGNFEHYVDYGFTASMEDELDSISRGEEDWVPVLDRFWG
                     GLSKQIADVEENVTRSDVAMAREIGTDPVSGRPVSVRYGRYGAFAQIGTRDDEEKPRF
                     ASLRPGQHIDDVTLEQALELFKLPRTLGSMPDGQPIKVAVGRFGPYAQYGTKKYVSLK
                     KEDDPYTITLERALQLIHEKQQFEASRTILDFAEAGIQVLNGRYGPYITDGKKNGKVP
                     KDRDPKSLTLEECT"
BASE COUNT          584 a         1051 c         1094 g          455 t
ORIGIN      
        1 gctcgatcca cagtccgctc tcgcggggct gtcaccgcct catccgccag ggcgcgaagc
       61 tcgtcgagac cgccgacgac atctacgccg aactcggcgc gctcgtcgcc ggcctcgggt
      121 ccttgcccgc ggccgaagcg actgagccgc aacaggattc cggcctctcg ttggacaagg
      181 actacgaaat cctgttagat gcgctcggct tcgagccggc cggcgtggac acgctcgtcg
      241 cgcgcaccgg gctcgaggca ggcgcggtgg cgtcgatgct gctcatcctc gagctcgagg
      301 ggcgcatcgc accgcagccc ggcggactct actgccggcg ccagccgggg aaaaaggcat
      361 gaaggaaggc gtcctcgaca tcctcatcta cctcttcgag aactatttcg acggcgagct
      421 ggatgagggc gaggagcccg accgcgaaac gctgaagcag gaactcgagc acgcgggatt
      481 cccgaccggg gaagtcgagc gggcgctgtg ctggctcgag gacctcgcgg ccgacccgtc
      541 gcgcacgggc ccggcaccga cggcgcgcgc gatccgcgtc ttcgacgcgc tggagcaggc
      601 gcggctcgac accgactgcc gcggcttcct cgtgcacctc gagcaggtcg gcatcctctc
      661 gcccacgcag cgcgagctcg tgatcgagag gctgatggcg ctcgacggcg acgacatcga
      721 catcgagcgt ctcaagtggg tggtgctgat ggtgctgttc agccagcccg gacaggaaac
      781 ggcgttctcc cgcatggagg acctggtgtt cgaggagcac tccggggcgg tccactgagg
      841 gccgcccggg gcggggtcag gacggatgcc gcggcccccc gcggcatgat cgtttctggg
      901 gctcgggaaa ttttgcaggg aaaatgagca ggaatctcgt catcgtcgag tcgccggcca
      961 aggccaagac catcaagaag tacctgggca aggacttcga ggtgatggcc tcctacggcc
     1021 acgtgcgcga cctcgtgccg aaggaaggag cggtggatcc ggcacggcgc ttcgccatgc
     1081 gctgggaatt gatcgacaag aaccgcaagc acgtcgacgc gatcgcgcgt gccgtgaaga
     1141 aggccgacgc cctgtacctc tcgacggacc cggaccgcga gggagaagcg atctcctggc
     1201 atctctacga actgctgaag gacgccggcg tgctcgacgg caaggccgtg catcgcagcg
     1261 tcttctacga gatcacgcag ggcgcggtcc aggacgcgat cgggcggccg cgcgagatcg
     1321 ccgaggagct ggtcaaggct tacctcgcaa ggcgggcatt ggactacctc gttggcttca
     1381 acatctcgcc gttgctgtgg aagaaggcgg gactgtcggg ggcatcggcc ggacgcgtgc
     1441 agagtccggc gctgcggatg atctgcgagc gcgaggaaga aatcaggctc ttcaagccac
     1501 gcgagtactg gaccgtcgac gccgaggccg agaagtcctc gcaggtgttc aacgcgcggt
     1561 tgacggagta tggcggccgg aaagtcgagc agttcacgat cacgagtgcc gcccaggcgg
     1621 acgaagtgcg tcgcacgctc gagcagtccg ccggcggtgc gctcgccgtc acggcggtcg
     1681 agcgcaagca gcgcaagcgc aatccatcgc cgccgttcac cacctccacg ctgcagcagg
     1741 aggccgcgcg caagctcggg ttcggcgcgc agcgcaccat gcgcacggcg cagaagctgt
     1801 acgagggcgt ggatttcggc gaaggcccgg tcggcctcat cacctacatg cgcaccgact
     1861 cggtcaacct cggcgtcgaa gccgtgcgcg agatccgcgg cgtgatcgga cggctctacg
     1921 gggcggaagc gctgcccgag tcgccacgcg tgtaccggac gaagtccaag aacgcccagg
     1981 aggcgcacga ggcgatccgc ccgaccgccg ccgcgcggac gccggagcag ctcgcgggcc
     2041 ggctcgatcc ggaccagcac aagctctaca cgctcgtctg gcgacgtgcg gtcgcgagcc
     2101 agatggaagc cgcggtcttc gataccgtgg ccgtggatct caccgccggc agcggccaca
     2161 cgctccgggc gaacggctcg accctggtca cgcccgggtt cctggccgta tatcgcgaga
     2221 gcttcgacga cgccaccctg gacgaggatg acgagaggcg tctcccgccg ctcgagacgg
     2281 gtgaccgtgt gatgctcaac gcgatcagcc cggcgcagca tttcaccgag ccgcctcccc
     2341 gctattcgga ggcgagtctc gtcaaggcgc tcgaggaata cggcatcggc cggccctcga
     2401 cctacgcgag catcatccag acgctgctct acaagaagta ctgcgagctg accaatcgcc
     2461 gcttcgtgcc gaccgacctc ggcaagatcg tcaaccgctt cctcaccggc aacttcgagc
     2521 actacgtgga ctacgggttc acggcgtcga tggaggacga gctcgacagc atctcgcgcg
     2581 gcgaggagga ctgggtgccg gtgctggatc gcttctgggg cgggctcagc aagcagatcg
     2641 ccgacgtcga ggagaacgtg actcgctccg acgtcgcgat ggcgcgcgag atcgggaccg
     2701 acccggtgtc gggccggccg gtgagcgtgc gctacggtcg ctacggtgcg ttcgcgcaga
     2761 tcggcacgcg cgacgacgag gagaagccaa ggttcgcgag cctgcggccc ggccagcaca
     2821 tcgacgacgt gacgctcgag caggcgctgg aactgttcaa gctgccgcgc acgctggggt
     2881 ccatgcccga cggccagccg atcaaggtcg ccgtcggccg cttcggtccc tacgcgcagt
     2941 acggcacgaa gaagtacgtc tcgctcaaga aggaagacga cccctacacg atcacgctgg
     3001 agcgcgcgct gcagctgatc cacgagaagc agcagttcga ggccagccgg acgatcctcg
     3061 acttcgcgga ggccggcatc caggtgctga acgggcgtta tggcccgtac atcaccgacg
     3121 gcaagaagaa cggcaaggtc ccgaaggatc gcgacccgaa gtcgctcaca ctcgaggagt
     3181 gcac
//
LOCUS       DAOMFD010000219         3172 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_15307_length_3172_cov_33.044594, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000219 DAOMFD010000000
VERSION     DAOMFD010000219.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3172)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3172
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15307_length_3172_cov_33.044594"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="PL152_09935"
     CDS             complement(<1..374)
                     /locus_tag="PL152_09935"
                     /inference="COORDINATES: protein motif:HMM:NF039479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6524 family protein"
                     /protein_id="HQR49648.1"
                     /translation="MGEFSFKGGLWRVAFALALVFATYNPSGYSYVHWLADGFPTVTP
                     VEAIVGLGLLGLWIFFVRSAHAALGVIGVAVLVALFAAIVWWMSTRGWVSVGTGSTLA
                     WVVLTILGLVLGLGMSWALIRQ"
     gene            482..2563
                     /locus_tag="PL152_09940"
     CDS             482..2563
                     /locus_tag="PL152_09940"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HQR49649.1"
                     /translation="MAHADAFARLNPAQREAATYGDTLPGGGVTAAPLLVVAGAGTGK
                     TSTLAHRVAHLLLHGAAPERLLLLTFTRRAALEMTRRAQRVVAETLARPGSAAAAPAA
                     FRLPWSGTFHAIGNRLVRQHAQRLGLDPSFNVLDRGDAADLMDVVRHRLGYSKAEKRF
                     PRKDTCLGIYSHRVNTQRSLRETLESAYPWCLDWEEELAALFRAYVEAKLAQQVLDYD
                     DLLLYWQLMVRDPALAADIGGQFDHVLVDEYQDTNALQAGILLRMKPDGRGVTVVGDD
                     AQAIYSFRGATVENILDFPEQFSPPAHVVTLEENYRSTQPVLDAANALMAEGGRQYRK
                     FLRSARSGGVKPRFVTVQDDQAQAGYVVERVLECRERGIDLRRQAVLFRNADHSDVLE
                     LELMRRNIPYVKYGGLKFLEAAHVKDLLAVLRWADNPRNRIAAFRVMQLLPGMGPANS
                     ERACDAFEAAGHRWPALGGHVVPAGAREHWRALVDLMVSLAGDAQWQGQVSRVRSWYE
                     PHLDRLYEAAPVRRGDLEQLERLSAQYPSRESFVTELTLDPPQASGDLSGDPLLDEDY
                     LVLSTVHSAKGQEWEATYVLNVTDGSFPSEFSTGRPELIDEERRLLYVAMTRARQELH
                     LVAPVRFYITQQAPGGDRYVHGARSRFLTDAVLQRLERATWPAEPEGVREGAAPSAAR
                     IDVATQLRNMW"
     gene            2584..3171
                     /locus_tag="PL152_09945"
     CDS             2584..3171
                     /locus_tag="PL152_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1134 domain-containing protein"
                     /protein_id="HQR49650.1"
                     /translation="MHRRRILRGIALMILALAGNVVVAAESASTSSSHEETYSRNEVL
                     AEAERFFGKGAKGLAEVIQKAFDKYGEPNAYIKGEEGAGAIGVGLRYGKGTLVWKGGG
                     SQPVWWQGPSVGFDIGGNAAKCFTLVYNLPSAERLFQRFPGVEGSLYFVGGFSLNYNR
                     TDDITLAPVRFGVGWRQGANIGYLHFTPKFSPIPI"
BASE COUNT          535 a         1072 c         1104 g          461 t
ORIGIN      
        1 ttctggcgga tcaacgccca cgacatgccg aggccgagca cgaggccaag gatcgtcagc
       61 acgacccacg ccagcgtgga accggtgccg acgctgaccc acccgcgggt cgacatccac
      121 catacgatgg ccgcgaacaa cgccaccagc acggcgacac cgatgacgcc gagggccgcg
      181 tgggcggagc gcacgaagaa tatccagagg cccagcagcc cgaggccgac gatggcttcg
      241 actggcgtga cggtcggaaa tccgtcggcc agccagtgca cgtaggagta gccggacggg
      301 ttgtaggtgg cgaacaccag cgcgagcgcg aacgccacgc gccacagccc gcccttgaag
      361 ctgaattccc ccatcgctgc tcccgatgcc tgccagcccg accgggcgcc atcttacagg
      421 ggcgaccggt cgccgccggt tactggatat ccatacagcc gcgaactata gtcccgatcg
      481 catggcccac gcggacgcat tcgcccgatt gaatcccgct cagcgcgagg cggcgaccta
      541 cggtgacacg ctgccgggcg gtggcgtgac tgccgcaccg ctgctcgtgg tcgccggtgc
      601 gggcacgggc aagaccagca ctctggcgca ccgggtcgca cacctgctgt tgcacggcgc
      661 agcgcccgaa cgcctgctgc tgctcacctt cacccggcgc gccgcgctcg agatgacccg
      721 acgcgcccag cgcgtggtcg ccgagaccct ggcccgcccg ggcagcgcgg ccgccgcacc
      781 ggcggccttc cggctgccgt ggtccggcac gtttcacgcg atcggcaatc gcctggtccg
      841 ccagcacgcg caacggctcg ggctcgaccc gtcgttcaac gtgctcgacc gcggcgacgc
      901 cgcggacctg atggacgtcg tgcgccatcg cctcgggtat tccaaggctg agaagcgctt
      961 tccgcgcaag gacacctgcc tcgggatcta ctcgcaccgg gtcaacacgc agcggtcgct
     1021 gcgggagacc ctcgagagcg cgtatccgtg gtgcctcgac tgggaggagg aacttgcggc
     1081 gctttttcgt gcatacgtcg aggcgaagct ggcgcagcag gtgctcgact acgacgacct
     1141 gctgctctac tggcagctca tggtgcgcga cccggcgctg gccgcggaca tcggcggcca
     1201 gttcgaccac gtgctggtcg acgagtacca ggacaccaat gcgctgcagg cgggcatcct
     1261 gctcaggatg aagccggacg ggcgcggcgt gacggtggtc ggcgacgacg cgcaggcgat
     1321 ctattcgttc cgtggcgcga cggtggagaa catcctcgac ttccccgagc agttctcgcc
     1381 gcccgcgcac gtcgtcacgc tcgaggagaa ttaccgctcg acgcagccgg tgctggatgc
     1441 ggcgaatgcg ctgatggcgg aaggcgggcg tcagtaccgg aaattcctgc gcagcgcgcg
     1501 cagcggcggc gtgaagccgc gcttcgtgac cgtgcaggac gaccaggccc aggccggcta
     1561 cgtggtcgag cgcgtgctcg agtgccggga gcgcggcatc gacctgaggc gccaggccgt
     1621 gctgttccga aacgcggacc acagcgacgt gctcgaactc gagctgatga ggcgcaacat
     1681 cccttacgtg aagtacggcg gcctcaagtt cctcgaagcc gcgcacgtga aggacctgct
     1741 ggccgtgctg cgctgggcgg acaacccgcg caatcgcatc gcggcgttcc gtgtcatgca
     1801 gctgctgccc ggcatgggac ccgcgaattc cgaacgcgcg tgcgacgcgt tcgaagccgc
     1861 aggccatcgc tggccggcgc tgggcgggca cgtcgtcccg gccggggcgc gggagcactg
     1921 gcgagcgctc gtggatctca tggtgtcgct ggcgggcgac gcgcaatggc aagggcaggt
     1981 gtcccgcgtg cgatcctggt acgagccaca cctggaccgg ctgtacgagg ctgcgccggt
     2041 gcgtcgcggc gacctcgagc agctcgagcg gctgtccgcg cagtacccgt cgcgcgagtc
     2101 gttcgtcacc gagctcacgc tggatccacc gcaggcgagc ggcgacctct ccggtgaccc
     2161 gttgctcgac gaggactacc tggtgctgtc caccgtgcac tcggcgaagg gccaggagtg
     2221 ggaggcaacc tacgtgctca acgtcacgga cggcagcttc ccgtcggaat tttcgaccgg
     2281 gcgcccggaa ctcatcgacg aggaacggcg tctactctac gtggcgatga cgcgcgcacg
     2341 gcaggaactg cacctggtcg cgcctgtgcg cttctacatc acgcagcagg cgccgggcgg
     2401 cgatcgctac gtgcacggtg cgcgcagccg cttcctgacg gacgcggtgc tgcagcgcct
     2461 cgagcgcgcg acctggccgg cggagccgga aggcgtgcgc gagggagcgg cgccctctgc
     2521 agccaggatt gacgtcgcaa cgcagttgcg gaacatgtgg tgaacggacg ggaggaactt
     2581 cagatgcacc gcaggcggat actccggggc attgccctga tgatcctggc gctcgccggg
     2641 aacgtggtgg tcgcggcaga gtccgcgagc accagttcgt cgcacgagga gacgtactcg
     2701 cgcaacgagg tgctcgccga ggccgagaga ttcttcggca agggcgcgaa gggcctcgcc
     2761 gaggtgatac agaaggcctt cgacaagtac ggcgagccca atgcgtacat caagggagaa
     2821 gaaggcgccg gcgcaatcgg agtgggcctg cgctacggca agggcacgct cgtctggaag
     2881 ggcggcggat cgcagccggt gtggtggcag ggcccgtcgg tcgggttcga catcggcggc
     2941 aatgccgcca agtgcttcac gctcgtctac aacctgccga gcgccgagcg actgttccag
     3001 cgattcccgg gcgtcgaggg cagcctgtac ttcgtcggtg gcttcagcct gaactacaac
     3061 cgcaccgacg acatcacgct cgccccggtg cgcttcggcg tcggctggcg gcagggcgcg
     3121 aacatcgggt acctgcactt cacgccgaag ttcagtccga tcccgatcta gc
//
LOCUS       DAOMFD010000220         3152 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_15490_length_3152_cov_31.679690, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000220 DAOMFD010000000
VERSION     DAOMFD010000220.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3152)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3152
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15490_length_3152_cov_31.679690"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2061
                     /locus_tag="PL152_09950"
     CDS             <1..2061
                     /locus_tag="PL152_09950"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HQR49651.1"
                     /translation="CVEKVASDPAIRGAVIGSAKSSFMAGADIKDMAGAFSRGLTAAE
                     AAKFSGELNRLLRRMETCGKPFAAAINGVALGGGFEVALACHYRVMSEDPKAGVGFPE
                     VTIGLLPGAGGTQRTPRLIGVTEALRLISEGKPLKAQDALKKGLVNEVAPTGELVERA
                     RQWILKGGEGVQPWDKKGFRVPGGAGMMSPAAAQTFMVGTALAAKTTMRNYPAVAAIL
                     SCVYEGTILPIDAALAVESKYFGTLLAGPVARNLMRTMFINKGLADKLARRPAGPAKS
                     QVKKLGMLGAGMMGAGIAHVSAVAGMEVVLLDSTQELAEKGKDYSRQLLAKDVSRGKR
                     TQADAEAVLARIKPTTSYDELAGCDLVIEAVFENRDIKADVTRKTEAVIPKGSVFASN
                     TSTLPITGLAQASKRPAQFIGIHFFSPVEKMPLVEVILGKKTNDETLARALDYVAQIR
                     KTPIVVNDSRGFYTSRCFGTFVNEGIAMLQEGVAPALIENCAKLVGMPVGPLAVADEV
                     SIDLSWKIMRQTEQDLGRKFQKPVTYDTVRKFVEELKRPGKRFGAGFYDYPKDAKKHL
                     WPGLTGVFPRAAVQPPVNDIEKRFLCIQALEAARCMEEGVVTTASEADLGSILGWGFP
                     AWTGGTLSYIDTVGIREFVAECDRLAKRYGKRFKPSKWLRERAARGESFHGAVGATGA
                     DKAA"
     gene            2278..>3152
                     /locus_tag="PL152_09955"
     CDS             2278..>3152
                     /locus_tag="PL152_09955"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: protein motif:HMM:NF015047.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HQR49652.1"
                     /translation="MPIPAAFRDRLTLPVIGAPMFIASTPDLVIEQCKAGIVGAFPAL
                     NARPAPVLDEWLTRIQRELGDYASANPGAKVAPFAVNQIVHASNDRLMQDLETCVRHQ
                     VPLMITSLRPPREIVEATHSYGGLVFHDVINLKHARKAAEQGVDGIIVVCAGAGGHAG
                     MGSPFALVREIRQFFDGTIILSGAMSSGADVFAAIALGADFAYIGTRFLATTEANVPD
                     AYKQSIVDSNSDEVVYTSLFTGVKGNYLSRSVAAAGFDPANLPEADKSKMNFGSGGNT
                     EKKAWRDIWSAGQGVG"
BASE COUNT          542 a         1026 c         1109 g          475 t
ORIGIN      
        1 tgcgtggaga aggtcgcttc cgacccggcc atccgtggcg cggtgatcgg ctcggcgaag
       61 tcgagcttca tggcgggcgc cgacatcaag gacatggccg gtgccttctc gcgcgggctc
      121 acggctgccg aggccgcgaa gttcagcggc gagctcaatc gcctcctgcg gcgcatggag
      181 acgtgcggca agccgttcgc cgcggcgatc aatggcgtgg cgctcggcgg cggcttcgaa
      241 gtggcgctcg cctgtcacta ccgcgtgatg tcggaggacc cgaaggccgg cgtcggcttc
      301 ccggaggtca cgatcgggtt gctgcccgga gcgggcggca cgcagcgcac gccccgcctg
      361 atcggcgtga ccgaggcact gcggctcatc agcgaaggca agccgctcaa agcccaggac
      421 gcgctcaaga agggcctcgt gaacgaggtc gcgcccaccg gcgagctggt cgagcgcgcc
      481 cggcagtgga tactgaaagg cggagaaggc gtccagccgt gggataagaa aggtttcagg
      541 gtcccgggcg gtgccgggat gatgtcgccg gcagccgcgc agaccttcat ggtcggcacg
      601 gcgctcgcgg ccaagaccac gatgcgcaac tacccggcgg tggccgcgat cctgtcgtgc
      661 gtgtacgaag gcacgatcct gccgatcgac gcggcactcg ccgtggagtc gaagtacttc
      721 ggtacgctgc tggccggtcc cgtcgcgcgc aacctgatgc gcacgatgtt catcaacaag
      781 gggctcgccg acaagctcgc gcgccggccc gccggtcctg caaagtcaca ggtgaagaag
      841 ctcggcatgc tcggtgcagg gatgatgggc gcaggcattg cccacgtgtc cgcggtcgcc
      901 ggcatggagg ttgtgctgct cgacagcacg caggagctcg cggagaaagg caaggattac
      961 tcgcgacagc tgctcgcgaa ggacgtgagc cgcggcaaga ggactcaggc cgacgccgag
     1021 gcggtgcttg cgcggatcaa gcccacgacg agctacgacg aactggcggg ctgcgacctc
     1081 gtgatcgagg cggtgttcga gaaccgcgac atcaaggcag acgtgacgcg caagaccgag
     1141 gccgtgatcc cgaagggctc ggtgttcgcg agcaacactt ccacgctgcc gatcaccgga
     1201 ctcgcgcagg cgtcgaagcg cccggcgcag ttcatcggca tccacttctt ctcgccggtc
     1261 gagaagatgc cgctggtcga ggtcatcctc ggcaagaaga ccaatgacga gacgctggcg
     1321 cgcgccctcg actacgtcgc acagatccgc aagaccccga tcgtcgtcaa cgacagccgc
     1381 ggcttctaca ccagccgctg cttcggcacc ttcgtcaacg aaggcatcgc gatgctgcag
     1441 gagggcgtgg cgcccgcgct gatcgagaac tgcgccaagc tggtcggcat gccggtggga
     1501 ccgctcgcgg tcgccgacga ggtgtcgatc gacctgagct ggaagatcat gcggcagacc
     1561 gagcaggacc tcgggcgcaa gttccagaag ccggtcacct acgacacggt gcgcaagttc
     1621 gtcgaggagc tcaagcggcc tggcaaacgc tttggggctg gcttctacga ctacccgaag
     1681 gatgcgaaga agcacctgtg gccgggcctg acgggcgtgt tcccgcgcgc ggccgtgcag
     1741 ccgccggtga acgacatcga gaagcgattc ctgtgcatcc aggcgctcga ggcggcgcgc
     1801 tgcatggagg agggcgtcgt gaccacggcc tccgaagcgg acctgggctc gatcctcggc
     1861 tggggattcc cggcgtggac gggcggcacg ctgtcgtaca tcgacacggt gggcatccgc
     1921 gagttcgtgg cggagtgcga ccggcttgcc aagcgctacg ggaagcgctt caagccgtcg
     1981 aagtggctgc gggagcgcgc cgcgcgcggc gagtcgttcc acggtgcggt tggcgccacg
     2041 ggcgctgaca aggccgcata gaacagcgtc gctcggtcgc ggcgtgccag gtcattcttc
     2101 gcagggacgc cgtgaaccca tccgtggggg ctccgggcgc ggcgtccatg ccgcgcacgg
     2161 ccctgcgaag aacgacctga cccgccacgc ccgagtgcgg ctcgttgcgg ctgattgtga
     2221 tgccggcagg cgtcggccat cgcgcgttgt tctggcgacg tgaccgacgg agttttcatg
     2281 ccgatacctg cagcctttcg cgatcgcctg accctgccgg tcatcggcgc gccgatgttc
     2341 atcgcctcca cgcccgacct cgtgatcgaa cagtgcaagg cgggcatcgt cggggccttt
     2401 cccgccctca atgcgcggcc cgcaccagtc ctcgacgaat ggctgacgcg catccagcgc
     2461 gagctgggcg attacgcctc ggccaatccg ggcgccaagg tggcgccgtt cgccgtgaac
     2521 cagatcgtgc acgccagcaa cgaccggctg atgcaggacc tcgagacctg cgtgcggcac
     2581 caggtgccgc tgatgatcac gagcctgcga ccgccgcgcg agatcgtcga ggcgacgcat
     2641 tcctacggcg ggctggtgtt tcacgacgtc atcaacctca agcacgcgcg caaggcggcg
     2701 gagcagggcg tcgacggcat catcgtcgtc tgcgcaggcg cgggtggcca cgcgggcatg
     2761 ggcagcccgt tcgcactggt gcgggagatc cgccagttct tcgacggtac gatcatcctc
     2821 tccggcgcga tgagcagcgg tgccgacgtg ttcgccgcga tcgcactcgg cgcggatttc
     2881 gcctacatcg ggacccgatt cctcgcgacg accgaggcga acgtcccgga cgcctacaag
     2941 cagtcgatcg tggactcgaa ttcagacgag gtcgtctaca cgtcgctgtt cacgggcgtg
     3001 aaggggaact acctctcgcg cagcgtggcc gcggccgggt tcgacccggc gaacctgccc
     3061 gaggccgaca agtcgaagat gaacttcggt tccggcggca acaccgagaa gaaggcgtgg
     3121 cgcgacatct ggagcgcggg ccagggcgtc gg
//
LOCUS       DAOMFD010000221         3142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_15586_length_3142_cov_34.176547, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000221 DAOMFD010000000
VERSION     DAOMFD010000221.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3142
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15586_length_3142_cov_34.176547"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..880
                     /locus_tag="PL152_09960"
     CDS             <1..880
                     /locus_tag="PL152_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49653.1"
                     /translation="WLADNPGGAVFVTGLLGLLPLFGLGFAFFLPGAVPALVVLARGP
                     RLGFAIATGAAALLTVAMWMFGRPVAVGVIYSAWVLGPPLLLAVLYARTGSLSLCLQV
                     AVLAGVALLVLLHQTLGDPEQFWAPFVRELAQEMQRRGLPMDLEKDGLVEALARTLWG
                     WVTALTMVLAMSALFLARWWQTLPDRRGQFGREFQDLRLGLVLGTAGAILIGWSMWTD
                     QALVDDLERIFLGALMLVGLAAAHRFKAMGRLGGAWLWAIYILLVLVAPLMVAALAGW
                     GFVDNWLRSRARAQNA"
     gene            915..1364
                     /gene="rplI"
                     /locus_tag="PL152_09965"
     CDS             915..1364
                     /gene="rplI"
                     /locus_tag="PL152_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HQR49654.1"
                     /translation="MEVILLQKVANLGKIGDKVKVKPGYGRNFLLPQGKAALATAANL
                     KKFEERRAELEKVAADELSSATLRAGQLEGFRLKLTAKAGGEGKIFGSIGTADIAEAL
                     SAAGIRVERSEVRLHSGPIRVVGEHHVKLHLHSDVIVDLPVDVVGEE"
     gene            1439..2797
                     /gene="dnaB"
                     /locus_tag="PL152_09970"
     CDS             1439..2797
                     /gene="dnaB"
                     /locus_tag="PL152_09970"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HQR49655.1"
                     /translation="MTDTDALRVPPHSVEAEQAVLGGLLLDNSTWDSIADRLGADDFY
                     RRDHQQIFAAIAELAARSEPADAVTLAEYLAAKGLAEETGGLAYLAGLARDTPTAANI
                     RAYADIVRERALLRHLIRVSGEIAASAYASEGRTAKELVDDAERRVFEIAEAGRRTGS
                     GFVPLKDILGATIDRLDMLHENQGQLTGVATGYADLDRMTAGLQPGDLIIVAGRPSMG
                     KTTLALNIAENAAISANVPVAVFSMEMSREQLSMRMISSLGRVDQSHLRTGMFGDEDW
                     ARINSAIAQMKTAPVYIDDSGALTPTEVRARARRLSRERGLGLIVVDYLQLMQVSGTK
                     ENRATEISEISRSLKALAKELKVPVIALSQLNRGVEQRTDKKPVMSDLRESGAIEQDS
                     DLILLIYREEVYEPETPRKGIADIIVAKQRNGPTGEVHLTFLGKYTRFENLATGDYDY
                     GG"
     gene            2823..>3142
                     /locus_tag="PL152_09975"
     CDS             2823..>3142
                     /locus_tag="PL152_09975"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HQR49656.1"
                     /translation="MQTLIRAIIDTAALRHNLGRVREIAPEAHVMAVIKANGYGHSLH
                     IAARALADADGLAVARLEEALALRAAGFAGRILLLEGVFDAGQLAAAAQEKFAIVVHS
                     FEQLR"
BASE COUNT          488 a         1043 c         1136 g          475 t
ORIGIN      
        1 ctggctcgcc gacaatcccg gcggggcggt attcgtcacc ggcctgctgg ggctgctgcc
       61 gctgttcggc ctcggcttcg cgttcttcct gccgggggcc gtgcctgcgc tcgtcgtcct
      121 ggcgcgcggg ccgcggctcg gattcgcgat tgcgaccggc gcggcggcct tgctgaccgt
      181 cgcgatgtgg atgttcggtc ggccggttgc agtcggcgtg atctactcgg cctgggtgct
      241 cgggccgccg ctgctgctgg cggtgctgta cgcaaggacc ggctcgttga gcctgtgcct
      301 gcaggttgcc gtgctggcgg gagtggcctt gctggtgctg ttgcaccaga cgctcggcga
      361 cccggaacag ttctgggcgc ctttcgtgcg cgaactcgcg caggagatgc agcgccgcgg
      421 cctgccgatg gacctcgaga aggacgggct ggtcgaggcg ctggcgcgca cgctgtgggg
      481 ctgggtcacg gcgctgacga tggtgctcgc gatgagcgcg ctgttcctgg cgcggtggtg
      541 gcagacgctg ccggaccgga gaggccagtt cggacgcgag ttccaggacc tgcgtctcgg
      601 gctggtgctg gggaccgcgg gcgcgatcct gatcggctgg tcgatgtgga cggaccaggc
      661 gctggtggac gatctcgaga ggatctttct cggcgcgctg atgctggtgg gcctcgcggc
      721 cgcgcatcgc ttcaaggcca tgggacggct gggtggcgcg tggctgtggg cgatctacat
      781 cctgctggtg ctcgtcgcgc cgctgatggt cgcggcgctc gcgggctggg gattcgtgga
      841 caactggctg cgctcgcggg cgcgggcgca gaatgcgtga aattcgggac tgaagacaga
      901 tcgaggaacc tacgatggaa gtgatcctgc tgcagaaggt cgccaacctc gggaagatcg
      961 gcgacaaggt caaggtgaag cctggctacg gccgcaattt cctgctgccc cagggcaagg
     1021 ccgcgctcgc gacggccgcg aacctgaaga aattcgagga gcgccgcgcc gaactcgaga
     1081 aggtcgccgc cgacgagctg tcgagcgcca cgctgcgcgc gggccagctc gagggcttcc
     1141 gcctgaagct gaccgcgaag gcgggcggcg aaggcaagat cttcggctcg atcggcacgg
     1201 cggacatcgc cgaggcgctc tcggccgccg gcatccgcgt cgagcgcagc gaagtgcgcc
     1261 tgcactccgg cccgatccgc gtcgtgggtg agcaccacgt caagctgcac ctgcacagcg
     1321 acgtgatcgt cgacctgccg gtcgacgtgg tcggcgagga ataggtcctc gtccgccgcg
     1381 gtccggcccg atggaacagg acgcgggtga cgccaggggc cgcaggcgac tgcggcccgt
     1441 gaccgacacg gacgccctca gggtcccgcc tcactcggtc gaggcggagc aggccgtgct
     1501 cggcggcctg ctgctcgaca acagcacctg ggattccatc gcggaccggt tgggcgccga
     1561 cgacttctac cggcgcgacc accagcagat cttcgccgca atcgcggaac tcgccgcgcg
     1621 cagcgagccc gcggatgcgg tcacgctggc cgagtacctc gccgccaagg ggctcgccga
     1681 ggagacgggg gggctcgcct acctggccgg cctggcgcgc gataccccga cggctgccaa
     1741 catccgtgcg tacgccgaca tcgtccgcga gcgggcgctg ctccggcacc tgatccgcgt
     1801 gagcggcgag atcgcggcga gcgcctacgc gagcgaaggc cgcaccgcca aggaactcgt
     1861 cgacgacgcc gagcggcggg tgttcgagat cgccgaggcc ggacgccgca ccggttcagg
     1921 attcgtgccg ctgaaggaca tcctgggcgc gacgatcgac cggctcgaca tgctccacga
     1981 gaaccagggc cagctcacgg gcgtggcgac cggctacgcg gacctcgacc ggatgaccgc
     2041 cggcctgcag cccggcgacc tcatcatcgt cgcgggccgc ccctcgatgg gcaagaccac
     2101 gctggcgctc aacatcgccg agaatgccgc catctccgcc aacgtgccgg tggcggtctt
     2161 ctcgatggag atgtcgcgcg agcagctctc gatgcgcatg atctcgtcgc tcggccgcgt
     2221 ggaccagagc cacctgcgca ccggcatgtt cggcgacgag gactgggcgc gcatcaacag
     2281 cgccatcgcg cagatgaaga ccgcgcccgt ctacatcgac gacagcggcg cgctcacgcc
     2341 gaccgaggtc cgcgcgcggg cacggcggct gtcgcgcgag cgcggcctcg ggctcatcgt
     2401 cgtcgactac ctgcagctca tgcaggtgtc cggcaccaag gagaaccgcg cgaccgagat
     2461 ctcggagatc tcgcgctcgc tcaaggcgct cgcgaaggaa ctcaaggtgc cggtgatcgc
     2521 gctgtcgcag ctcaatcgcg gtgtggagca gcgcaccgac aagaagccgg tgatgtcgga
     2581 cctgagggaa tcgggagcga ttgaacagga tagcgacctg atcctgctga tctatcgcga
     2641 ggaagtgtac gagcccgaga ctccgcgcaa gggcatcgcc gacatcatcg tcgcgaagca
     2701 gcgcaacggc ccgacgggcg aagtgcacct gacgttcctc ggcaagtaca cgcgcttcga
     2761 aaatctcgcg accggcgact acgactacgg cggctgagac cgccgccggc gcaggcaatc
     2821 ccgtgcagac cctgatccgg gccatcatcg acacggcggc gctccggcac aacctcggcc
     2881 gggtgcgcga gatcgcgccc gaagcgcacg tcatggcggt gatcaaggcg aacggctacg
     2941 gccattcgtt gcacatcgcg gcacgcgcgc tcgccgatgc cgacgggctt gcggtggcgc
     3001 gtctcgaaga agcgctcgca ttgcgagccg cgggctttgc tggccgcatc ctgctgctcg
     3061 aaggtgtgtt cgacgctggc cagctggctg cagccgcgca ggagaagttc gcgatcgtgg
     3121 tccacagctt cgagcagctg cg
//
LOCUS       DAOMFD010000222         3130 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_15695_length_3130_cov_36.287154, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000222 DAOMFD010000000
VERSION     DAOMFD010000222.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3130)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15695_length_3130_cov_36.287154"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /gene="lipA"
                     /locus_tag="PL152_09980"
     CDS             <1..822
                     /gene="lipA"
                     /locus_tag="PL152_09980"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HQR49657.1"
                     /translation="VREHRLATVCEEARCPNIGECWNAGTATLMLMGEVCTRACRFCA
                     VDTGNPRGWLDEHEPENAARSVELMGLKYVVLTSVNRDDLEDGGAGHFAACVRAIKRV
                     NPQTAVEALTPDFEGVLADVETVVDSGVDVFAQNVETVRRLTHPVRDPRASYEQTLSV
                     LAHAKKRRPSLLTKTSLMLGLGETDSEVLQTMDDLRRVGVDILTLGQYLRPTVHHLAV
                     ERFVAPAEFESYRAAALERGFLECVAGPLVRSSYRAEQALARNNAGLGEGGLRRP"
     gene            822..1487
                     /gene="lipB"
                     /locus_tag="PL152_09985"
     CDS             822..1487
                     /gene="lipB"
                     /locus_tag="PL152_09985"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:NF010922.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033819 - lipoyl(octanoyl) transferase
                     activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HQR49658.1"
                     /translation="MPPPILRWLGLVEYEPTWRAMQRHVDERQAQDADEFWFLEHPPV
                     FTLGMNSRPEHLLSTGEIPVVQIDRGGQVTYHGPGQLVVYPLVDLARAGLGVRALVEG
                     IERAIVGTLAQWGIEAIGRRDAPGVYVQGRKIASVGLRIRHARSYHGLALNVAMDLSP
                     FRRINPCGYAGLEMTQVSELGGPADLEVVAAALAPRLLAALNLPLPGQSATSTWRQDV
                     SST"
     gene            complement(1442..1705)
                     /locus_tag="PL152_09990"
     CDS             complement(1442..1705)
                     /locus_tag="PL152_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006760320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HQR49659.1"
                     /translation="MTEETLLEFPCDFPIKVMGAATDEFRSLALGIITRHFGSPAAGS
                     IEERPSSGGKYLGLTITVRAESKAQLDAAYVELTSCRQVLVAL"
     gene            complement(1709..2581)
                     /locus_tag="PL152_09995"
     CDS             complement(1709..2581)
                     /locus_tag="PL152_09995"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HQR49660.1"
                     /translation="MAAPYPTCYLDGAWLPLAEARISPLDRGFLFADGVYEVVPVHRG
                     RPFRLREHLQRLDDSLRAIRITNPHDPAGWAAVLGRLATEAGDRELLLYLQVTRGAEA
                     GRNHLLPKDVTQTVFAFASPYPAPSPTVMQQGLRAVTLEDIRWDRCDIKSVALLGNIL
                     LRQEAADRGADEALLVRDGRLLEGSSSSVFLCAGGTLVTPPNSHRILPGTSRDAVLEL
                     AEGWLPRQVADIDARDIAGCDELWISSAGRGVLPVTTVDGQPVGTGRPGPLWEEMYSR
                     LQRHLDDIASTPAL"
     gene            complement(2583..>3130)
                     /locus_tag="PL152_10000"
     CDS             complement(2583..>3130)
                     /locus_tag="PL152_10000"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF019555.2"
                     /note="penicillin-binding protein 5; removes C-terminal
                     D-alanyl residues from sugar-peptide cell wall precursors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HQR49661.1"
                     /translation="FTWNGITQQNRNGLLWRDPSVDGVKTGHTETAGYCLIASAKRDG
                     MRLVSVVMGTDSMKAREDASASLLGYGFNFYETKRVYGAGQALTTARVWKGAESEVGL
                     VLHHDLYVTGQRGQIGSVQAQFELPARLVAPLAAGTAVGKVKVVVDGKPVATYDLYPA
                     EDVTAGGIFRRAIDTVRLWFK"
BASE COUNT          457 a         1079 c         1118 g          476 t
ORIGIN      
        1 gtgcgcgagc accggctcgc gaccgtttgc gaggaggcca ggtgcccgaa catcggcgaa
       61 tgctggaatg ccggcacggc cacgctgatg ctgatgggcg aggtctgcac gcgggcctgc
      121 cgattctgcg ccgtggacac cggcaatccg cgcggctggc tggacgagca cgagccggag
      181 aacgcggcgc gcagcgtcga gctgatgggc ctgaagtacg tcgtgctgac ctcggtcaac
      241 cgcgacgatc tcgaggacgg cggtgcgggc cacttcgccg cctgcgtgcg cgccatcaag
      301 cgtgtgaacc ctcagacggc cgtggaagcg ctgacaccgg acttcgaggg cgtgctcgcc
      361 gacgtggaga cggtcgtcga cagcggtgtc gacgtattcg cacagaacgt cgagaccgtg
      421 cgccggttga cgcaccccgt gcgcgacccg cgcgcgtcct acgagcagac gttgtcggtg
      481 ctcgcgcacg cgaagaagcg ccgcccgtcg ctgctcacca agaccagcct gatgctcggc
      541 ctgggagaga ccgactcgga ggtcctccag acgatggacg acctccgccg ggtcggcgtg
      601 gacatcctca ctctcgggca gtacctgcgc ccgaccgtgc accacctcgc cgtggagcgg
      661 ttcgtcgctc ccgcggagtt cgagtcgtat cgagcggcgg cgctcgagcg cgggttcctc
      721 gagtgcgtcg ccgggccgct ggtgcgctcg agctatcgcg ccgagcaggc gctcgcgcgg
      781 aacaacgcag gactcggcga gggcgggctc cgccggccgt gattccgcct ccgatcctcc
      841 gctggctcgg cctcgtcgag tacgagccga cgtggcgcgc gatgcagcgt cacgtggacg
      901 agcggcaggc gcaggacgcc gatgaattct ggttcctcga gcatccgccg gtgttcacgc
      961 tcgggatgaa ctcgagaccc gagcacctgc tctcgaccgg tgagatcccc gtggtgcaga
     1021 tcgaccgcgg cgggcaggtg acctaccacg gtccgggaca gctggtcgtg tacccgctgg
     1081 tggacctggc gcgcgccggc cttggcgtgc gggccctcgt ggagggcatc gagcgcgcga
     1141 tcgtcggcac gctcgcgcaa tgggggatcg aggcgatcgg ccgacgcgac gcgcccggag
     1201 tgtacgtcca gggccgcaag atcgcgagcg tgggcctgcg catccgtcac gcgcggtcgt
     1261 accacggcct cgcgctcaac gtggcgatgg acctgtcgcc gttccgccgc atcaatccct
     1321 gtggctacgc gggcctcgag atgacgcagg tgagcgagct gggtggtccc gcggacctcg
     1381 aggtggtggc cgccgcgctc gcgccccggc tcctggcagc gctgaacctg ccgctgccgg
     1441 gtcagagtgc gaccagtacc tggcgacagg acgtgagttc gacgtaggcc gcgtcgagct
     1501 gcgccttgct ctcggcgcgc acggtgatcg tgaggccgag gtacttgccg ccgctgctcg
     1561 gccgttcctc gatgctgcca gcggcgggac tcccgaagtg tcttgtgatg atgccgagtg
     1621 ccaggctgcg gaactcgtcg gtcgccgccc ccatcacctt gatcgggaag tcgcagggaa
     1681 actcgagcag cgtctcctcg gtcatcgctc agagcgccgg cgtggacgcg atgtcgtcga
     1741 ggtggcgctg cagtcgcgag tacatttcct cccacagggg accgggtcgg ccggtgccga
     1801 caggctggcc atccaccgtg gtgaccggga gcacgccgcg gccggccgac gagatccaga
     1861 gttcgtcgca gcccgcgatg tcgcgggcat cgatgtccgc gacctgacga ggcaaccatc
     1921 cctccgccag ctccagcacc gcgtcccgcg acgtccccgg caggatgcgg tggctgttcg
     1981 gcggcgtcac cagcgtcccg ccggcgcaca gaaacacgct gctcgacgag ccctcgagca
     2041 ggcgcccatc gcgcacgagc agcgcttcgt cggcgccgcg atcggcggcc tcctgcctga
     2101 gcaggatgtt cccgagcagc gcgaccgact tgatgtcgca gcggtcccag cggatgtcct
     2161 cgagcgtcac ggcccgcagg ccttgctgca tgacggtcgg cgacggcgcc gggtaggggc
     2221 tcgcaaacgc aaacaccgtc tgcgtcacgt ccttcggcag gaggtggttg cgacctgctt
     2281 ccgcgccccg cgtcacctgc aggtacagca gcaattcgcg gtcgccggcc tcggtggcga
     2341 ggcgaccgag cacggccgcc cagcccgccg ggtcgtgggg attggtgatg cggatcgcgc
     2401 gcaggctgtc gtccagtctc tgcaggtgct cgcgcaggcg gaacgggcgc ccgcggtgca
     2461 ccgggaccac ctcgtacacg ccgtcggcga acaggaagcc acggtcgagc ggcgagatgc
     2521 gcgcctcggc cagcggcagc cacgccccgt ccaggtagca ggtcgggtag ggggcagcca
     2581 tgtcacttga accagaggcg caccgtgtcg atggcgcggc ggaagatgcc gccagcggtg
     2641 acgtcctccg ccgggtacaa gtcgtaggtc gcgacgggct tgccgtcgac gacgaccttg
     2701 accttgccga cggccgtgcc ggccgcgagt ggcgcgacga ggcgtgccgg cagctcgaac
     2761 tgcgcctgca cgctgccgat ttggccgcgc tggccggtca cgtacaggtc gtggtgcagc
     2821 acgaggccca cctccgactc ggcgcccttc cagacccgcg ccgtggtgag cgcctggccg
     2881 gcgccgtaga cgcgcttcgt ctcgtagaag ttgaatccgt agccgagcag cgacgcgctg
     2941 gcatcctcgc gggccttcat cgagtcggtg cccatcacca ccgacacgag gcgcatgccg
     3001 tcacgcttgg cggaggcgat caggcaatag cccgcagtct cggtgtgtcc ggtcttcacg
     3061 ccgtcgacgc tcgggtcgcg ccacagcagg ccgttgcggt tctgctgcgt gatgccgttc
     3121 caggtgaatt
//
LOCUS       DAOMFD010000223         3105 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_15918_length_3105_cov_30.897705, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000223 DAOMFD010000000
VERSION     DAOMFD010000223.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3105)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3105
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15918_length_3105_cov_30.897705"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1623
                     /locus_tag="PL152_10005"
     CDS             136..1623
                     /locus_tag="PL152_10005"
                     /inference="COORDINATES: protein motif:HMM:NF015692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HQR49662.1"
                     /translation="MTATAQETVEQDPPRPIPECYWVVPGRLLVGEYPGSASRASAMD
                     RLRRFTDAGVTCFIDLTELAELPGYEPLLPMQTQDGRRIEYLREPIPDHGVPANPEDM
                     HQAVATLDGALQAGHVVYLHCRAGIGRSALVAGCWLAGQLGPQGDPIAILRERWRQCA
                     KSAAWPVVPETDEQEAYVRRWQAQAQQPSRPQAAASSAEERVRGALLGLAAGDDAGQA
                     REAGRAPNGLWTQHTALTLCLAESLLECGGPDPRDQIERYLRCHRDGHLSPDGRPMAV
                     TPDVAKALANYQWRQKALAGPHDPRDRSTASLPRVLAAVAFARTDAPSAVRLAGESSR
                     TTHQSPFVIDACRYFGALLVGALRGAASDAFGAGLYEPEPGLWVERPLKPAVAAAFRS
                     RSVEISQAVPDPPRADAIQAVMRARDAVISGRGFDETVRAAAAHAAEPALEAALTGAL
                     YGAVHGERAVTGEAVSRLARRELIESFASRLSQSGSVARGWEPKG"
     gene            1620..2693
                     /locus_tag="PL152_10010"
     CDS             1620..2693
                     /locus_tag="PL152_10010"
                     /inference="COORDINATES: protein motif:HMM:NF027219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative zinc-binding metallopeptidase"
                     /protein_id="HQR49663.1"
                     /translation="MTRAAAGTRKRPGGKVARRRREPAWARLDDEALLDKRLCDLRLG
                     LAGTRLEDGVRRLYGELAAKGIRFRPHFWLSEEWFSPDGVPGIAIPFYLAHPRLMRLE
                     RRYMHEVEGGNDKWLMRILRHETGHAIDTAFTLRRRKAWRRTFGKASRRYPSRYSPRP
                     ASQNFVLHLGHWYAQSHPTEDFAETFAVWLPRRSRWRSQYQGWPALAKLEFVDATMRG
                     LEGRRAVHLNRDTIEPLASSTRTLREHYRRKQARYEVNLARSYDRRLLRVFGRAEDHP
                     HRPVASRFLRTVQPQLKRFLVRRARMHPYLVSHVLRTATQRARSLDLRLKASQRRTKR
                     EVLGMVERIMIDMLLRDRENFAL"
     gene            2690..>3105
                     /locus_tag="PL152_10015"
     CDS             2690..>3105
                     /locus_tag="PL152_10015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49664.1"
                     /translation="MKMLRVLVLVHETLVPPEDLKGYSEQEIESWRTEYDVMTSLRAM
                     GHEVRVLGLGDNLADLRNAITDWKPDIAFNLLEEFQGIVTYDQYVVAFLELMRLPYTG
                     CNPRGMMISRDKVLSKQILSYHRIPTARFALLPRNRR"
BASE COUNT          467 a         1080 c         1113 g          445 t
ORIGIN      
        1 tgatcctggc gaccggcttg cgggccacga gttgcttcag cacctggagc gccccctgta
       61 ttgctcttga tccaagtctc tcacagccct gccgccgtcc gcagcaccgg ttgcggctgc
      121 cgtatcattc gctccatgac cgcgactgcc caggagaccg tcgagcagga tccgccccgg
      181 ccgatcccgg agtgctattg ggtggtgccg ggccggctgc tggtgggcga gtatccgggc
      241 tcggcatcgc gcgcatctgc gatggaccgc ctgcgccgct tcaccgacgc cggcgtgacc
      301 tgcttcatcg acctcaccga gctggccgag ttgcccggct acgagcccct gctgcccatg
      361 cagacgcagg acgggcgccg gatcgagtac ctgcgcgagc cgattccgga ccacggcgtg
      421 ccggcgaacc cggaggacat gcaccaggcg gtcgccacgc tcgacggcgc gttgcaggcg
      481 gggcacgtcg tgtacctgca ttgccgcgcc ggcatcgggc gcagcgcgct ggtcgccggg
      541 tgctggctcg cgggccagct cggtccgcag ggcgatccaa tcgcgatact tcgtgaacgc
      601 tggcgacaat gcgccaagtc agcggcgtgg ccggtggtcc cggagacgga cgagcaggag
      661 gcctacgtcc gccggtggca ggcgcaggct cagcaaccgt cacgcccgca ggctgcggcg
      721 tcgagcgccg aggagcgagt gcgcggtgcg ctgctcggac tcgccgcggg cgacgacgca
      781 ggtcaggcac gggaggcggg tcgagcgccg aacgggctgt ggacccagca tacggcgctc
      841 acgctgtgcc ttgcggagag cctgctcgag tgcggcggcc ctgacccgcg cgaccagatc
      901 gagcgctacc tgcgctgcca ccgcgacggg cacctgtcgc cggacggtcg tcccatggcg
      961 gtcacgcccg acgtcgccaa ggccctcgcc aactaccagt ggcggcagaa ggcgctggcg
     1021 gggccacacg acccgcgcga ccgcagcacg gcgagtctgc cgcgagtgct cgccgccgtc
     1081 gcattcgcgc ggacggatgc gcccagcgcg gtccgcctgg ccggtgaatc ctcgcgcacg
     1141 acgcaccagt cgccattcgt gatcgatgcg tgccgatact tcggcgcgtt gctggtcggg
     1201 gcgctgcgcg gagcggcgtc cgacgcattc ggcgctggct tgtacgagcc ggagccggga
     1261 ctctgggtcg agcggccgtt gaagccagca gtggccgccg catttcggtc acgcagcgtg
     1321 gagatctcgc aggccgtgcc ggacccgccg agagccgatg cgatccaggc cgtcatgcgc
     1381 gcgcgggacg ccgtaatttc cggccgcggg ttcgatgaga ccgtgcgcgc ggccgcggcg
     1441 cacgcggccg agccggcgct cgaggcggca ttgacgggag cactgtacgg cgccgtgcac
     1501 ggcgagcgtg ccgtcacggg cgaagcggtg tcgcgactgg cgcggcgcga actgatcgag
     1561 tcgttcgcct cgcggctgtc ccagtcgggc tccgtcgcac gcgggtggga accgaaggga
     1621 tgacgcgggc cgccgcaggg acgcgcaagc gaccgggcgg gaaggtcgcc cgccgtcgac
     1681 gggagcccgc gtgggcccgc ctcgacgacg aggcactgct cgacaagcga ctctgcgacc
     1741 tgcgcctcgg cctcgccggc acccggctag aagatggcgt gcggcggctg tacggcgaac
     1801 ttgccgcgaa gggcatccgc ttcaggccgc acttctggct gtccgaggag tggttctcgc
     1861 cggacggcgt gccgggcatc gcgattccgt tctacctcgc ccatccccgg ctgatgcggc
     1921 tcgagcgccg ctacatgcac gaggtcgagg gcggcaacga caagtggctg atgcgcatcc
     1981 tgcgccacga gaccggacac gcgatcgaca cggcgttcac gctgcgccgg cgcaaggcgt
     2041 ggaggcgcac gttcggcaag gcgtcgaggc gttatccgtc ccgctactcg ccgcgcccgg
     2101 cgagccagaa tttcgtcctg cacctcggcc actggtacgc gcagagccat ccgacggagg
     2161 acttcgccga gacgttcgca gtctggctgc cgcggcgctc gcgctggcgc agccagtacc
     2221 agggctggcc tgcgctggcc aagctcgagt tcgtcgacgc gacgatgcgc gggctcgagg
     2281 ggcggcgcgc ggtgcacctc aaccgcgaca cgatcgagcc gctcgcgtcg agcacccgca
     2341 cgctgcgcga gcattatcgc cgcaagcagg cgcgctacga agtgaatctc gcgcgctcct
     2401 acgaccggcg actgctgcgc gtattcggcc gggcggagga tcacccgcac cgcccggtgg
     2461 cgagccggtt cctgcgcacg gtccagccgc agctgaagcg gttcctggtg cggcgggcgc
     2521 gcatgcaccc gtacctggtg agccacgtgc tgcggacggc cacgcagcgg gcgcgctcgc
     2581 tcgacctgag gctgaaggcg agccagcgcc ggaccaagcg cgaggtgctc ggcatggtcg
     2641 agcgaatcat gatcgacatg ctgctgcgcg atcgggagaa cttcgcacta tgaagatgct
     2701 gcgcgtgctg gtgctggtgc acgagaccct ggtgccgccc gaggacctca agggctattc
     2761 ggagcaggag atcgaaagct ggcgcacgga atacgacgtg atgacgtcgc tcagggccat
     2821 ggggcacgag gtcagggtgc tcgggctggg cgacaacctc gccgacctgc gcaacgcgat
     2881 caccgactgg aagcccgaca tcgcgttcaa cctgctcgag gaattccagg ggatcgtcac
     2941 ctacgaccag tacgtggtgg ccttcctcga gctgatgagg ctgccctaca ccggctgcaa
     3001 cccgcgcggc atgatgatct cgcgcgacaa ggtgctctcc aagcagatcc tgtcctacca
     3061 ccgcatcccg acggcgcgct tcgcgctgct gccgcgcaac cggcg
//
LOCUS       DAOMFD010000224         3102 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_15946_length_3102_cov_31.536265, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000224 DAOMFD010000000
VERSION     DAOMFD010000224.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3102)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3102
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15946_length_3102_cov_31.536265"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="PL152_10020"
     CDS             <1..536
                     /locus_tag="PL152_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HQR49665.1"
                     /translation="GQLGEVMQESIQAAMTVVRSRAAVLGLEPEFYQKADVHIHVPEG
                     ATPKDGPSAGIGMCTALVSALTRSAVRSDVAMTGEITLRGEVLPIGGLKEKLLAAHRG
                     GIRTVLIPDENVKDLAEIPDNIKGNLDIKPVKWIDEVLQLALRNMPQPAVVTSAAEDA
                     KPAKRPGRRQAKPVHAH"
     gene            554..841
                     /locus_tag="PL152_10025"
     CDS             554..841
                     /locus_tag="PL152_10025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQR49666.1"
                     /translation="MQEQAMNKNELVEAVSDRTGLARSDAAQAVEAVLAAVTEALRAG
                     DTVALSGFGTFVAKARAARTGRNPRTGEAIAIPASRAPAFKAGKALKDALN"
     gene            861..936
                     /locus_tag="PL152_10030"
     tRNA            861..936
                     /locus_tag="PL152_10030"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:894..896,aa:Val,seq:tac)
     gene            946..1022
                     /locus_tag="PL152_10035"
     tRNA            946..1022
                     /locus_tag="PL152_10035"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:980..982,aa:Asp,seq:gtc)
     gene            1096..3006
                     /locus_tag="PL152_10040"
     CDS             1096..3006
                     /locus_tag="PL152_10040"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HQR49667.1"
                     /translation="MLQQIRDKITGWFASLFLGAIAVVFIFWGIRFESGPRAAAAKVN
                     GETISVEVVRRAWQDRLSQLQQAARNELPEAVIKTEQQKLLEEFVNREVLQQHVQAMG
                     YRVSDADIAQEIQAIPALQVDGKFSRDRYAALLRQQGESEAQFEQQFRRDLQIGQLRN
                     GIGISAFTTVGELRRRVALEGETREVDWYTVAAAPYASQVAVTPAEVQAYYDQHKATY
                     VTPETVSLQYVELKAADVASTVSVTDEALRQYYDQVAPERYVDPERRRARHILVETGK
                     DDAAARKRADELYARAKAGEDFARLASENSDDAGSKAQGGELGWATKESYVPAFADAL
                     FSMQKGEIRGPVKTEFGYHIIELEDVQPAHQRGFDEVRADLEKDYRNEQAQSLFYEKS
                     QQLADDAFASLSELESVAKKQGLQLMTVEGFTRKGGGPFGDNKKVIDAVFSDEVLQER
                     QNSPAIDVANDEVVVLRVTDYKPSVQRPLEEVRPEIEAALREQGARKAAEAAAKASAD
                     RITGATPFADVAAAAGAVPVAPAKVDRTAEGVPPELLKAIFAAPRPAPGKSTGGTAVL
                     ANGDVAVFVVNAVHAGVAPTGDTAAIELSQVARKGQNSAAIAEFRAYVKELERTAKVK
                     LNENVFSNDEGQ"
BASE COUNT          557 a         1035 c         1102 g          408 t
ORIGIN      
        1 ccggccagct cggcgaggtg atgcaggagt ccatccaggc cgcgatgacc gtggtccgca
       61 gtcgcgccgc tgtgctgggc ctcgagcccg agttctacca gaaggccgac gtccacattc
      121 acgtgccgga gggcgccacg cccaaggacg gcccgagcgc cggcatcggc atgtgcacgg
      181 cgctggtctc cgcgctgacc cgcagcgcgg tgcgcagcga cgtcgcgatg accggcgaga
      241 tcacgctgcg cggcgaggtg ctcccgatcg gcgggctcaa ggagaaactg ctggccgcgc
      301 atcgcggcgg catccgcacg gtcctgatcc cggacgagaa cgtgaaggac ctcgccgaga
      361 tcccggacaa catcaagggc aacctcgaca tcaagccggt gaaatggatc gacgaagtgc
      421 tgcagctggc gctcaggaac atgccgcaac ccgcggtcgt gacatccgct gcagaagacg
      481 ccaagccggc caagcgaccc ggccgccggc aggccaagcc ggttcacgcg cactgacgtc
      541 caggcgcgaa cacattcagg agcaagcgat gaacaagaac gaactcgtgg aagcggtgag
      601 tgatcgtacg ggactcgcgc ggtccgatgc cgcgcaggcg gtggaagcgg tgctcgccgc
      661 ggtcacggag gcgctgcgcg ccggcgacac cgtggcgctc tcgggcttcg gcaccttcgt
      721 tgcgaaggcc cgcgcggccc gcaccggccg caacccgcgc accggcgagg cgatcgcgat
      781 ccccgcaagc cgcgcgcccg cgttcaaggc tggcaaggca ctgaaggatg ccctaaacta
      841 gcgcgctccc cagggggcgc gggtgcttag ctcagctggg agagcgtcgc ccttacaagg
      901 cgaaggtcgg gggttcgatc ccctcagcac ccaccagtcg cacgcggagc ggtagttcag
      961 ttggttagaa taccggcctg tcacgccggg ggtcgcgggt tcgagtcccg tccgctccgc
     1021 cagtttacgt agcgcaagcc cgcccacgcg ggctttcttg tataagaacg caccaacagt
     1081 caccacgcac atcccatgct gcagcagatc cgcgacaaga tcaccggctg gttcgcctcg
     1141 ctgttcctcg gcgcgatcgc cgtggtgttc atcttctggg gcatccgctt cgagtccggc
     1201 ccgcgcgccg ccgccgcgaa ggtgaacggc gagaccatct ccgtcgaggt ggtgcgccgc
     1261 gcctggcagg accggctctc gcagctgcag caggctgcgc gcaacgagtt gccggaggcc
     1321 gtgatcaaga cggagcagca gaagctgctc gaggaattcg tcaatcggga ggtgctgcag
     1381 cagcacgtcc aggcgatggg ctatcgcgtg agcgacgccg atatcgcgca ggagatccag
     1441 gcgatcccgg ccctgcaggt ggacggcaag ttctcgcgcg accgctatgc cgccctgctg
     1501 cgccagcagg gcgagagcga ggcccagttc gagcagcagt tccgccgtga cctgcagatc
     1561 gggcaactcc gcaacggcat cggcatctcg gcattcacca cggtcggcga gctccgcagg
     1621 cgtgtcgcgc tggagggcga gacccgcgag gtcgactggt acacggttgc tgcggccccg
     1681 tatgcgtcgc aggtcgcggt caccccggcc gaggtccagg cgtactacga ccagcacaag
     1741 gccacttacg tgacgcccga gaccgtgtcg ctgcagtacg tggagctcaa ggccgccgac
     1801 gtcgcctcca cggtctccgt caccgacgag gccctgcgcc agtactacga ccaggtggcg
     1861 ccggagcgct acgtggatcc ggagcggcgc cgcgcgcggc acatcctcgt cgagaccggc
     1921 aaggacgacg cggcggcgcg caagagggcc gacgagttgt acgcgcgcgc caaggccggc
     1981 gaggatttcg ccaggctcgc gagcgagaac tccgacgacg cggggtcgaa ggcgcagggc
     2041 ggcgagctcg gctgggcgac caaggaaagc tacgtcccgg cgttcgcgga cgcgctgttc
     2101 tcgatgcaga agggcgagat ccgcggtccg gtcaagaccg agttcggcta ccacatcatc
     2161 gagctcgagg acgtgcagcc ggcgcaccag cgcggcttcg acgaggtgcg tgccgacctg
     2221 gagaaggact accgcaacga gcaggcgcag tcgctgttct acgagaagtc gcagcagctg
     2281 gccgacgacg cgttcgcctc gctgagcgaa ctcgagagcg tggcgaagaa gcagggcctg
     2341 cagctcatga ccgtggaggg attcacgcgc aagggcggcg gtccgttcgg cgacaacaag
     2401 aaggtcatcg acgcggtgtt cagcgacgag gtgctgcagg agcggcagaa cagcccggcg
     2461 atcgacgtcg ccaacgacga agtggtggtg ctccgggtga cggactacaa gccgtcggtg
     2521 cagcgaccgc tcgaggaagt gcgccctgag atcgaggcgg ccctgcgcga gcagggcgcc
     2581 cgcaaggctg ccgaggccgc cgccaaggcg agcgccgaca ggatcaccgg cgcgacgccg
     2641 ttcgccgatg tcgcggcggc agccggtgcc gtgccggtcg cgcctgccaa ggtggaccgg
     2701 acggcggagg gcgtgccacc ggagctgctc aaggcgatct tcgccgcgcc gcggcctgcg
     2761 ccgggcaagt ccacgggcgg gacggcggtg ctggcgaacg gcgacgtcgc agtgttcgtc
     2821 gtgaacgccg tgcacgccgg ggttgcgccg accggggaca ccgccgcgat cgagctctcg
     2881 caggtcgcgc gcaaggggca gaactcggcc gcgatcgccg agttcagggc ctacgtgaag
     2941 gaactggagc gcacggccaa ggtcaagctg aacgagaacg tgttcagcaa cgacgaaggg
     3001 cagtaggcag cgaccggacc ctccatcgag ggccatggtc gcgcgaatca gccgcccttc
     3061 tcgtcctccg ggaacgccat gcccacgtgg ctgtatccgg ca
//
LOCUS       DAOMFD010000225         3076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16169_length_3076_cov_32.102284, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000225 DAOMFD010000000
VERSION     DAOMFD010000225.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3076
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16169_length_3076_cov_32.102284"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1666)
                     /locus_tag="PL152_10045"
     CDS             complement(<1..1666)
                     /locus_tag="PL152_10045"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HQR49668.1"
                     /translation="MSRGRRSATALAAVVVAACTLLLGTARAPSASTGANGADLAPTE
                     REAKVSRLVSSMFERSHYRQAPVNDPVSSLVLDRYLESLDGNRSYFLASDVQEFERYR
                     YQLDDAITQGKLEPAFAIFNRFQQRSRERMAYALASLKTEPDFTLQESFEFDREHAPW
                     ATSQAELDEIWRKRVKNDALSLMLTGKTWAEASDILQKRYERAAKRSEQVTSDDVFES
                     FMNSFASVFDPHSNYFSPRNSEEYRIQMSLSYEGIGASLQVVDDYVTIMEILPGGSAQ
                     QSGELKATDRILAVGQGKTGEMVDVVGWRLDDVVDLIRGPGGSFVRLQIQSGNAAPGA
                     AERVVTLTRKKITLEAQAAKKELRTIKRGDQELRIGVINVPSFYQDFDARTAGDKNYR
                     STTRDVRRLIEEFQKEGGIDGLVLDLRENGGGHLTEAIGLVNLFVPKGPVVQLREAGG
                     RVEVLESEDQGVAYSGPLTILVDRFSASASEIFAAAMQDYGRGIVIGQETYGKGSVQN
                     LYPLDRYALGQDPGYGQLTVTIGMYYRVTGDSTQNRGVQPDIRLPSA"
     gene            1803..2096
                     /locus_tag="PL152_10050"
     CDS             1803..2096
                     /locus_tag="PL152_10050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49669.1"
                     /translation="MRPLTVLTGIVLGSSAATTFGLGATLVVFLVLSGDYPQFRAELP
                     LLTLYVALFAGLTALAAVSFIGQARERPWRRRAQFAMWGALVALAALYWSTRS"
     gene            complement(2141..2398)
                     /locus_tag="PL152_10055"
     CDS             complement(2141..2398)
                     /locus_tag="PL152_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49670.1"
                     /translation="MKSRHTLRWILAALIFGTAVLPCLVYLTGVRILGPYGPGGVGAF
                     YGMFLRDLAQLRPAAWTLALGPAVLVLAWRVVTAFAWADDA"
     gene            2460..>3076
                     /locus_tag="PL152_10060"
     CDS             2460..>3076
                     /locus_tag="PL152_10060"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HQR49671.1"
                     /translation="MQAVRTAVIGVGYLGRFHAQKYASLPESRLVAVVDADPATRDRV
                     AAEVGCRAVADFRELLGEVDAVSIATPTPAHFALASECLERGIHVLVEKPVTETLDEA
                     RRLVEIAARRGCVLQVGHLERFNPAITALEGTLKTPRFVESHRLAPFKERGTDVNVVL
                     DLMIHDIDLIQSLVRAPIVSIDAVGTSVFSGELDIANARIRYANG"
BASE COUNT          439 a         1069 c         1057 g          511 t
ORIGIN      
        1 tcgcggaggg caggcggatg tccggctgga cgccgcgatt ctgcgtgctg tcgccggtga
       61 cccggtagta catgccgatc gtgaccgtga gctggccgta gcccgggtcc tggccgagcg
      121 catagcggtc gagcggatac aggttctgca cggagccctt gccgtaggtc tcctgcccga
      181 tcacgatgcc gcgcccgtag tcctgcattg ccgcggcgaa gatctccgag gccgacgcgc
      241 tgaagcggtc gaccaggatg gtgagcgggc ccgagtaggc gacgccctgg tcctcggatt
      301 ccagcacttc gacgcggcct ccggcctcgc gcagctgcac gaccgggccc ttgggcacga
      361 acaggttgac gaggccgatg gcttcggtga ggtgcccgcc gccgttctcg cggaggtcga
      421 gcaccagccc gtcgatgccg ccttccttct ggaattcctc gatcaggcgt cgcacgtcgc
      481 gcgtggtgct gcggtagttc ttgtcgcccg ccgtgcgcgc gtcgaagtcc tggtagaagg
      541 acgggacgtt gatcacgccg atcctgagct cctggtcgcc gcgcttgatc gtgcgcagtt
      601 ccttcttcgc ggcctgcgcc tcgagcgtga tcttcttgcg cgtgagcgtg acgacgcgct
      661 cggccgcgcc cggcgcggcg ttgcccgact ggatctgcag gcgcacgaac gagccgcccg
      721 ggccgcggat caggtcgacg acgtcgtcga gccgccaccc cacgacgtcg accatctcac
      781 ccgtcttgcc ctggcccacg gcgaggatgc ggtcggtcgc cttcaactcg ccgctctgct
      841 gcgcggaccc gcccggcagg atctccatga tcgtcacgta gtcgtcgacg acctgcagcg
      901 atgcgccgat gccctcgtag gagaggctca tctggatgcg gtattcctcg gaattgcgcg
      961 gcgagaaata gttcgagtgc gggtcgaaga cgctcgcgaa cgagttcatg aagctctcga
     1021 acacgtcgtc cgaggttacc tgctcgctgc gcttggccgc gcgctcgtag cgcttctgca
     1081 ggatgtcgct cgcctcggcc caggtcttcc cggtcagcat cagggacagg gcatcgttct
     1141 tgacgcgctt gcgccagatc tcgtcgagct cggcctggga cgtcgcccac ggggcgtgct
     1201 cccggtcgaa ttcgaacgat tcctgcagcg tgaagtcggg ctccgtcttg agcgaggcga
     1261 gcgcgtacgc catgcgctcg cggctgcgct gctggaagcg gttgaagatc gcgaatgcgg
     1321 gctcgagctt gccctgcgtg atggcgtcgt cgagctggta gcggtagcgc tcgaactcct
     1381 gcacgtcgct cgcgaggaaa tagctgcggt tgccgtcgag cgactcgaga taacggtcga
     1441 gcacgaggga cgacacgggg tcgttgaccg gcgcctggcg gtaatgggag cgctcgaaca
     1501 tactgctcac gagccgggag accttggcct cgcgctcggt cggcgcgagg tcggctccgt
     1561 tcgccccggt cgacgccgac ggcgcacggg cggtcccgag cagcagcgtg caggcggcca
     1621 cgaccacggc cgccagggcc gtcgcgctgc gccggccgcg gctcaccggc cgcgacttcg
     1681 attcgtcccc atgacctgcc tgatacactt tcccggaccc tcggctcgtt tttgccacca
     1741 gctaggctag agacaccccg gccgcgactc aagcgtcgtc gtgtcgggca cccgcgacaa
     1801 ccgtgcgacc gctcaccgtt ttgaccggaa tcgtcctcgg atcctcggcc gccaccacgt
     1861 tcggcctcgg ggcgaccctg gtcgtgttcc tggtgctctc cggcgactac ccgcagtttc
     1921 gcgccgagct gccgctgctg accctgtacg tcgcactctt cgcaggtttg accgcgctcg
     1981 cagcggtcag tttcatcggc caggcgcgcg agcgcccgtg gcggcgccgg gcccagttcg
     2041 ccatgtgggg cgcgctcgtc gcgctcgccg cgctgtactg gtccacgcgc agttgacccc
     2101 cccgggggca gggccggggc tcgatggccg gcggcgacct tcaggcgtcg tcggcccagg
     2161 cgaatgcggt gaccacccgc caggcgagga ccaggaccgc cgggccgagc gccagcgtcc
     2221 aggcggccgg ccgcagctga gccaggtcgc gcaggaacat gccgtagaag gccccgacgc
     2281 cgccgggacc gtatggaccg aggatgcgga ctccggtcag gtagaccagg cacggcagca
     2341 cggcggtgcc gaagatcagc gccgcgagga tccagcgcag ggtgtggcgc gacttcatcg
     2401 acggcatcct gagtccggcc ggcgcgaacc gcaatagccc attcggtatg atcgccggca
     2461 tgcaggctgt tcgaaccgcc gtcatcggcg tgggctacct cggccggttc cacgcgcaga
     2521 agtacgcgtc gctgccggag tcgcggctcg tggccgtggt cgacgccgac cccgcgacgc
     2581 gggatcgcgt tgccgcggag gtcgggtgcc gcgcggtcgc tgatttccgc gagctgctcg
     2641 gcgaggtcga cgcggtcagc atcgccacgc cgacgccggc acacttcgcc ctggcgagcg
     2701 aatgcctcga gcgcggcatc cacgtgctgg tcgagaagcc cgtcaccgag acgctcgacg
     2761 aagccaggcg cctggtcgag atcgcggcgc gccgcggctg cgtgctgcag gtcggtcacc
     2821 tcgagcggtt caatcccgcg atcaccgctc tcgagggcac gctcaagacg ccgcgcttcg
     2881 tggagtcgca ccgcctcgcg ccattcaagg agcgcggcac cgacgtcaac gtcgtgctgg
     2941 acctcatgat ccacgacatc gacctgatcc agagcctggt tcgcgcgccg atcgtgtcca
     3001 tcgacgcggt cgggacctcg gtgttctcgg gcgaactcga catcgccaac gcgcgcatcc
     3061 gctacgcgaa cggctg
//
LOCUS       DAOMFD010000226         3047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16467_length_3047_cov_31.723596, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000226 DAOMFD010000000
VERSION     DAOMFD010000226.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3047
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16467_length_3047_cov_31.723596"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..288
                     /locus_tag="PL152_10065"
     CDS             <1..288
                     /locus_tag="PL152_10065"
                     /inference="COORDINATES: protein motif:HMM:NF016269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSC84-related protein"
                     /protein_id="HQR49672.1"
                     /translation="YMTLVTVSAGLGMGVSKSHLIWVFETPDAYANFVNTGIEIGADA
                     NLTVNPGAGGGMYDGAVEASPGAWLYQLSDAGLAAELTVQGSKYFKDSGLN"
     gene            334..762
                     /locus_tag="PL152_10070"
     CDS             334..762
                     /locus_tag="PL152_10070"
                     /inference="COORDINATES: protein motif:HMM:NF015009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dirigent protein"
                     /protein_id="HQR49673.1"
                     /translation="MNRYGLLVLMLMLGTARASAGEVIAVVEHADTDATTDTGAKGDS
                     VGDILTFANPVFDKANKVKVGTDQGFCVRTVAGKAWECMWTLTLKDGQLTVEGPFLDA
                     GDSVMAVTGGTGKYAGSRGQMKLHARDAKGSAYDFVYELQ"
     gene            796..1146
                     /locus_tag="PL152_10075"
     CDS             796..1146
                     /locus_tag="PL152_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49674.1"
                     /translation="MSNSPRERGAVERALMERARMQAERDREPAARRWLQHAIALAAA
                     LVLVGIVMLGFDAFLTAMQKYMEAEVVDPAAAPATAPMPAYVVPGAVNPPPPEGPDPR
                     PSQAPAQGTSPATP"
     gene            complement(1067..1579)
                     /locus_tag="PL152_10080"
     CDS             complement(1067..1579)
                     /locus_tag="PL152_10080"
                     /inference="COORDINATES: protein motif:HMM:NF014912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HQR49675.1"
                     /translation="MKGKVPRTVDEFMAWAWAMEVEAVERYEELAEQMLTHHNAEVAT
                     LFARLARIEGKHRDQIAQQMGWTRPPDTGSFHWRTPEGPETTDYGDLHYLMQPYHALK
                     LAEHNEERAAQFFEHFAAAKLPADVRAAAAAMAAEEREHVRLIREWLAKYPEPEPGWD
                     EDPDPPAVAD"
     gene            complement(1576..2397)
                     /locus_tag="PL152_10085"
     CDS             complement(1576..2397)
                     /locus_tag="PL152_10085"
                     /inference="COORDINATES: protein motif:HMM:TIGR02177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HQR49676.1"
                     /translation="MTGMLTHADFKSDIKPVWCPGCGDFGVVNALTKALAKMELRPEN
                     VAVVSGIGCSSRIPAYTSCYGFHGVHGRALAAGTGLKVARPDLTVIVTGGDGDGYSIG
                     GNHFLHACRRNVDLTYIVMDNRVYGMTKGQPSPTTEPDWDSKLSPEGTGLREFHPMMM
                     ALTAGANFIARAFTGDPNGLADVIVQAVRHPGFSFVEVLSPCVTFRPEQRDWKKLVKH
                     DALKPATDIARAARRMLRDTGFDLGVLYAGDRRPYEPRGRREQRAVAALEARFTP"
     gene            complement(2394..>3047)
                     /locus_tag="PL152_10090"
     CDS             complement(2394..>3047)
                     /locus_tag="PL152_10090"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49677.1"
                     /translation="SPAIVLSDQFLGQARAVVDRPPDGDAPGTGPRVLAAAHEPGFLR
                     YKITANGISPMAIPGTAGVTYTADGLEHGERGAPSSQGADHLAQLEKRRRKLDLHDYG
                     PDWADVEGAGDLAVLTFGSCTGVVREGLSRARADGVAARLVSIRLLSPAQPERLQAAL
                     AGVRRVLVVEQNHSGQFYRHLRSEYDLPCPVTAWHRPGALQFSPGEIHRRLVDWSRS"
BASE COUNT          471 a         1024 c         1072 g          480 t
ORIGIN      
        1 tacatgacgt tggtgacggt atcggcgggt ctcggcatgg gcgtcagcaa gagccacctc
       61 atctgggtct tcgagacgcc ggatgcgtac gcgaacttcg tcaataccgg catcgagatc
      121 ggcgccgacg ccaatctcac ggtgaatccc ggcgcaggcg gcggcatgta cgacggtgcg
      181 gtcgaggcgt ctccgggagc gtggctgtac cagctttccg acgctggcct cgccgcggaa
      241 ctgaccgtcc agggcagcaa gtacttcaag gattcaggcc tgaactgacg ggacgcgttc
      301 atcacgttgg tacgacacgg ggggagacga atcatgaacc gctacggatt gctggtgctg
      361 atgctgatgt tgggaactgc acgggcctcg gccggcgaag tcatcgccgt cgtcgagcac
      421 gccgacacgg atgcgaccac ggacacgggc gcgaagggcg actcggtcgg ggacattctt
      481 acgttcgcga atccggtgtt cgacaaggcc aacaaggtca aggtcggcac ggaccagggc
      541 ttctgcgtgc gcaccgtcgc cggcaaggcg tgggaatgca tgtggacgct gacgctgaag
      601 gacggacagc tgacggtcga aggtccgttc ctcgacgcgg gcgattccgt catggccgtc
      661 accggcggga ccggcaagta cgcgggctca cgcggccaga tgaagctgca cgcgcgcgac
      721 gcgaagggct cggcgtacga cttcgtctac gaactgcagt aatcgcgagc gcgggctcgc
      781 gcgtcgcgaa gtgccgtgag caattcgcca cgggaacgcg gcgcggtcga gcgcgcgctc
      841 atggagcgcg cccgcatgca ggcggagcgc gaccgggagc cggcggcccg ccgctggctg
      901 cagcacgcga ttgcgctcgc agccgccctc gtgctcgtcg gcatcgtcat gctcgggttc
      961 gacgccttcc tgaccgcgat gcagaagtac atggaggcgg aggtcgtcga tccagcggcg
     1021 gcaccggcaa cggccccgat gccggcctac gtcgtgccgg gtgcggtcaa tccgccaccg
     1081 ccggagggtc cggatcctcg tcccagccag gctccggctc agggtacttc gccagccact
     1141 ccctgatcag ccggacgtgc tcgcgctcct cggccgccat cgcggctgca gcggcccgca
     1201 cgtcggcggg aagcttcgcc gcggcgaagt gctcgaagaa ctgcgcggct cgttcctcgt
     1261 tgtgctcggc gagcttgagc gcgtgatacg gttgcatcag gtagtgcagg tcgccgtaat
     1321 cggtggtctc cgggccctcg ggcgtccgcc agtggaacga tcccgtatcg ggcggccggg
     1381 tccaccccat ctgctgcgcg atctggtcgc ggtgcttgcc ctcgatgcgc gccagcctcg
     1441 cgaacagcgt cgcgacctcg gcgttgtggt gagtgagcat ctgctcggcg agttcctcgt
     1501 agcgctcgac agcctcgacc tccatggccc acgcccacgc catgaactcg tccacggtcc
     1561 gcggcacctt gcctttcacg gtgtgaacct cgcctcgagc gccgcgacgg cgcgctgttc
     1621 ccggcggccg cgcggctcat agggcctgcg atcacccgcg tagagcacgc ccaggtcgaa
     1681 gccggtgtcg cgcagcatcc gcctcgccgc ccgcgcgatg tccgtcgccg gcttgagcgc
     1741 gtcgtgcttc acgagcttct tccagtcgcg ctgctccggg cggaacgtca cgcagggcga
     1801 caacacctcg acgaacgaga acccggggtg ccgcaccgcc tgcacgatca cgtcggcgag
     1861 gccgttcgga tcgccggtga aggcgcgcgc gatgaagttc gcgccggccg tcagggccat
     1921 catcatggga tgaaactccc gcagccccgt tccctcgggc gacagcttcg agtcccagtc
     1981 gggttcggtc gtgggcgagg gctggccctt ggtcatgccg tacacgcggt tgtccatgac
     2041 gatgtaggtg aggtcgacgt tgcggcgaca ggcgtgcagg aagtggttgc cgccgatcga
     2101 gtagccgtcg ccgtctccgc cggtgacgat cacggtgagg tccggccgcg cgaccttgag
     2161 accggtgccg gcggcgagcg cgcgaccgtg cacgccgtga aagccatagc agctcgtgta
     2221 ggcagggatg cgcgacgagc agccgatgcc ggagacgacc gcgacgtttt ccggacgaag
     2281 ctccatcttc gcgagcgcct tggtgagcgc gttgaccacg ccgaagtcgc cgcagcccgg
     2341 gcaccagacc ggcttgatgt cggacttgaa gtcggcgtgc gtgagcatcc cggtcatgat
     2401 cgactccagt cgacgagcct gcggtggatc tctccggggc tgaactgcag cgcacccggc
     2461 cggtgccacg ccgtgaccgg gcacggcagg tcgtactccg aacgcaggtg gcggtagaac
     2521 tgccccgagt gattctgttc gacgaccagg acccttcgca ccccggccag ggccgcctgc
     2581 aggcgctccg gctgcgcggg cgacagcagc ctgatcgaga cgaggcgggc cgcgacgccg
     2641 tcggcgcgtg cgcgggacag tccttcccgg accacgccgg tgcacgatcc gaacgtcagc
     2701 acggccaggt cgcctgcgcc ctcgacgtcg gcccagtcgg gcccgtagtc gtggagatcg
     2761 agcttgcgac gacgcttctc gagctgcgcc aggtggtcgg cgccctgcga ggacggcgcg
     2821 ccgcgctcgc cgtgctcgag accgtcggcc gtgtaggtca cacccgcggt gcccggaatc
     2881 gccatcggtg agattccgtt cgcggtgatc ttgtagcgca ggaaacccgg ctcatgggcc
     2941 gcggcgagca ccctcggacc ggtgcctggt gcgtcgccat ccggcggccg gtcgacgacg
     3001 gcacgcgcct gcccgaggaa ctggtcggac agcacgatgg cgggcga
//
LOCUS       DAOMFD010000227         3041 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16517_length_3041_cov_32.350636, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000227 DAOMFD010000000
VERSION     DAOMFD010000227.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3041)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3041
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16517_length_3041_cov_32.350636"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..320)
                     /locus_tag="PL152_10095"
     CDS             complement(<1..320)
                     /locus_tag="PL152_10095"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HQR49678.1"
                     /translation="MNELKDLIVDVADFPKPGILFRDITPLLRRRFPETVGAVESLFT
                     AAEWDQVDAVAGIESRGFILAAALAERRRKGFVPIRKKGKLPPPVVDVAYDLEYGSGV
                     LEMQT"
     gene            426..1073
                     /locus_tag="PL152_10100"
     CDS             426..1073
                     /locus_tag="PL152_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016657 - oxidoreductase activity, acting
                     on NAD(P)H, nitrogenous group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HQR49679.1"
                     /translation="MSCRAFLPTPVPEATVREILDAARRAPSGGNLQPWRVYALAGEP
                     LAQLLAQVRGKLFSHPRGEGAEYSIYPENLGEPYRSRRFKCGEDMYATIGVPREDKPA
                     RLRQFAQNYAFFGAPVGLFFYIDRQMGPPQWADLGMFMQTVMLLARERGLHTCAQEAW
                     SVWYPTIGAFLQVPPELMLFSGMALGHRDESAPINRLRTDRADLDEFAGLRGFTS"
     gene            1381..1572
                     /locus_tag="PL152_10105"
     CDS             1381..1572
                     /locus_tag="PL152_10105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49680.1"
                     /translation="MSLSWIANRSLKFSPRVRLVDDDAAAALRRKLESLEWKPSNGFL
                     RDEIVTRPPSPAERPRSAA"
     gene            complement(1579..1929)
                     /locus_tag="PL152_10110"
     CDS             complement(1579..1929)
                     /locus_tag="PL152_10110"
                     /inference="COORDINATES: protein motif:HMM:NF023184.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HQR49681.1"
                     /translation="MTGRLDREFRTMQRMVEIHCADKHGAERGTLCADCVEFLGYVQR
                     RLEKCPYGEDKPTCARCPIHCYKPAQREMAREVMRYAGPRMPLSHPWLSLLHVLDKLR
                     RVEHPMETRRRERG"
     gene            complement(1938..2414)
                     /locus_tag="PL152_10115"
     CDS             complement(1938..2414)
                     /locus_tag="PL152_10115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012607273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HQR49682.1"
                     /translation="MALEIRSTAFESGGAIPSRFTCEGRDLSPPLEWQGLPRGTKSLV
                     LIVDDPDAPDPAAPKMTWVHWVLADLPPETKGLDEGVAPRALPAGTVAGVNDWGRTGY
                     GGPCPPVGRHRYFHKLYALDAVLALPQRPTKAQVEAAMRGHVLAQAELVGTYQKSR"
     gene            2716..>3041
                     /locus_tag="PL152_10120"
     CDS             2716..>3041
                     /locus_tag="PL152_10120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HQR49683.1"
                     /translation="MSAFAMWGLFPLYLKPLAAVPTLQVMGHRIVWCCILVFAWLAAR
                     GELGSVRSALASAGTRHRLAASAVCISLNWLIYVWAVANGHVVDASLGYFINPLFSVV
                     LGVFVLG"
BASE COUNT          464 a         1075 c         1004 g          498 t
ORIGIN      
        1 gtctgcatct cgagcacccc cgacccgtac tcgaggtcgt aggccacgtc gacgaccggc
       61 ggcggcagct tgcccttctt gcggatcggc acgaatccct tgcgccgccg ctcggccagc
      121 gccgcggcca ggatgaagcc gcgcgactcg atgcccgcga ccgcgtccac ctggtcccac
      181 tccgccgcgg tgaagagcga ctcgacggcg ccgacggtct cggggaaacg ccgccgcagc
      241 aacggcgtga tgtcgcggaa caggatgccc ggcttgggga aatccgcgac gtcgacgatc
      301 aggtccttca gttcgttcat gcgcacctcg gcccgcgagc ctagcgaaat tccccctcgc
      361 ccgcgagaat cccgcgccag cctggaggaa tcgcagtgaa cgtcaccgac gccgtcgaat
      421 cgcgtatgag ctgccgcgcc ttcctgccga cgcccgtgcc cgaggcgacg gtgcgcgaaa
      481 tcctcgatgc ggcgcggcgc gccccttcgg gcggcaacct gcaaccttgg cgggtgtacg
      541 cgctggcggg cgagcccctc gcccagctgc tcgcgcaggt gcgcggcaag ctcttctcgc
      601 acccgcgcgg ggaaggagcg gagtattcga tctatccgga gaacctcggc gagccatacc
      661 gctcgcgccg cttcaagtgc ggcgaggaca tgtacgccac catcggcgtg ccgcgcgagg
      721 acaagccggc gcgcctgcgc cagttcgcgc agaactacgc gttcttcggc gcaccggtcg
      781 gcctcttctt ctacatcgac cggcagatgg gcccgccgca gtgggcggac ctcggcatgt
      841 tcatgcagac cgtgatgctg ctggcacgcg agcgcggcct gcacacgtgc gcgcaggaag
      901 cgtggtccgt gtggtacccg acgatcggtg cattcctgca ggtgccgccg gagctcatgc
      961 ttttctcggg catggcgctc ggccaccgcg acgagtcggc cccgatcaac cgcctgcgca
     1021 ccgatcgcgc ggacctcgat gagttcgcgg ggctgcgcgg attcacgtcc tgacccggag
     1081 ggtgaccacc gcccgtcgga attcctgaca cataaatcca acaccctggc ggcgagttat
     1141 gtcgcaagga gcgcgcatcc agccccagaa cgtgaaccac gtcacgcgtt atggcgagtt
     1201 gttggtgtca cgcaacgacg attcgctgtc gtcccggttt actcccggca ggtgccggtg
     1261 gtcgccgccg gcccgtcaca cccgcgtaat cattggcatt caggctgtgc gggcacaact
     1321 ggcacacaac ctgcatcgat acacacgggc gcagcttcgc gcgcacgatg gagtgacggg
     1381 atgagtctca gctggatcgc caatcgcagc ctcaagttct cgccgagggt ccgcctggtc
     1441 gacgatgacg cggccgcggc cctgcgccgc aagctcgagt cgctggagtg gaagccgtcg
     1501 aatggtttcc tgcgcgacga gatcgtcacg aggccgccgt cgcccgcaga acgcccgcgc
     1561 agcgccgcct gagaggcgtc agccgcgttc ccgccggcgc gtctccatcg ggtgttcgac
     1621 gcgccgcagc ttgtccagga catggagtag gctgagccac ggatggctca agggcatgcg
     1681 cgggcccgcg tagcgcatca cttcgcgggc catctcgcgc tgcgccggct tgtagcagtg
     1741 gatcgggcaa cgcgcgcagg tcggcttgtc ctcgccgtac gggcacttct ccagccggcg
     1801 ctgcacgtag ccgaggaatt caacgcagtc tgcgcagaga gtcccgcgct cagcaccgtg
     1861 cttgtccgcg cagtgaatct ccaccatccg ctgcatggtg cggaactcac ggtccaatcg
     1921 tccggtcacg tgtctcgtca tcgcgacttc tggtaggtcc cgacgagctc cgcctgcgcg
     1981 agcacgtggc cgcgcatggc ggcttcgacc tgcgccttgg tcggccgctg cggcagggcg
     2041 agcaccgcat ccagcgcata gagcttgtgg aaataacgat ggcgaccgac gggaggacac
     2101 ggaccgccat acccggttcg cccccagtcg ttcacccccg cgaccgtgcc tgcgggcagc
     2161 gcacgcggcg ccacgccctc gtcgagtcct ttcgtctccg gcgggaggtc ggcgagcacc
     2221 cagtgcaccc acgtcatctt cggtgccgcc gggtccggtg catcggggtc gtccacgatc
     2281 agcacgagcg acttcgtgcc cctgggcagg ccctgccact cgaggggcgg cgagaggtcg
     2341 cggccctcgc aggtaaagcg cgacgggatg gcgccaccag actcgaatgc cgtggatcgg
     2401 atttcgaggg ccatgacgga acctccgtaa aggatcgtcg ccgcacatgc ggccgtcaga
     2461 cagcgattca tgcgcgcagg atgccgccgg agggctcgct caaccctcgt ggattgccgc
     2521 aggccaggcc ggtcgtcgcc agcaagcgtc agtgatgtcg acctgatcac cgagccgctc
     2581 cagggatccg gatctgcgag aatgccggcc ctcgtcccac tgccccgccg ggccgcgatg
     2641 gccgcctcga attcgaacga tacgacagtc gccgcagctg gacacgcccc ccgccgttcc
     2701 gggctcgcgg cggccgtgag cgcgtttgcg atgtggggcc tgttcccgct gtacctcaag
     2761 ccgctcgccg cggtgccgac actgcaggtc atgggtcacc gcatcgtctg gtgctgcatc
     2821 ctcgtgttcg cctggcttgc ggcgcgcggg gaactcggct ccgtccggtc ggcgctcgcc
     2881 agcgccggga cccgccatcg cctcgccgcg tcggccgtgt gcatcagcct gaactggctg
     2941 atctacgtct gggccgtggc gaacggccac gtggtcgatg cgagcctcgg ctatttcatc
     3001 aatccgctgt tcagcgtcgt gctcggcgtg ttcgtgctgg g
//
LOCUS       DAOMFD010000228         3026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16661_length_3026_cov_30.707842, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000228 DAOMFD010000000
VERSION     DAOMFD010000228.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16661_length_3026_cov_30.707842"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="PL152_10125"
     CDS             <1..699
                     /locus_tag="PL152_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HQR49684.1"
                     /translation="HAARAAQASTMSGPVRTGAPAVALSDRGQRRRLEILDAALRIMA
                     RDGLRAVSHRAVAAEAGVPLAATTYYFSDLEDLITEAFLHWSGSQLRVVGEFHAAALD
                     VVRRAGGERAGQAGLVDALADAASDYVVDQVLNHRPDRVLEFAFLHEAARLPRLRSAV
                     RERQMQDRRFLEQFHAAAGSPDPVLDAQISFSLLLGLEKAALLADPDALDREWVREVL
                     VHYLCNALSGGHRG"
     gene            793..>1238
                     /locus_tag="PL152_10130"
     CDS             793..>1238
                     /locus_tag="PL152_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR49685.1"
                     /translation="MNAGHVARLLLCVTMLSAAPSAGAASGKDIFAATCVACHGANAQ
                     GNPAVGAPALAGQAAAYLERQLGDFRTGLRGADPKDTYGSQMRAIAATLTDPAAVKAV
                     AAYLASLPPPIVKPAAGANLRNGNNVYQGKCGACHGGRAEGIAAMST"
     assembly_gap    1239..1338
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1339..2526
                     /locus_tag="PL152_10135"
     CDS             <1339..2526
                     /locus_tag="PL152_10135"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HQR49686.1"
                     /translation="GMVMNQDAAASLITLSERCPPSFEKTAQGTCELRDLYQFYDSLE
                     GAGLGGTQTGLPPNRDGFTPAEIDLGRYLFFDPLLSGDGTLSCASCHRPEQGLSDGRP
                     RSIGVHGQDVGRAAPTLWNVAFLKSFFWDARAGSLEAQAVGPLFSSKEMGNTPERLLA
                     GLNGNADYRRLFADAFPALRGKPIALPQVERALAAFQSSLISLNSRYDRYAQGYHAAL
                     SEHEIEGLNVFRSFVARCAECHTPPLFTNQQVAVIGAPEPKGRPFDVGAEATFHAAKL
                     KGGFKVPTLRNIARTAPYMHSGAFGTLRDTVVFYNKGRGNAVPKGMTLYLHWHIGSPH
                     LTDYEVDRLVDFMQALTDETFMPEIPARVPSGLRPIDNPPQGAAATRTAMAQEKNTRG
                     EFR"
     gene            2523..>3026
                     /locus_tag="PL152_10140"
     CDS             2523..>3026
                     /locus_tag="PL152_10140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49687.1"
                     /translation="MKKGLVLAVVAAIAAFAGGAWYGRTSKSAMVVTSGSGGASYTGG
                     FKADSAAAAAEKAGTIRSVAGPAPQKEAIVKDGESIQAAVKAASAGTIIRVMPGTYHE
                     TVYVDKDDIRIIGVIDAGRRPVLDGEGKLNDAVLYSGNNFVVENLTIRNYKGNGIMGQ
                     AGNNFEIR"
BASE COUNT          478 a         1022 c         1011 g          415 t
ORIGIN      
        1 cacgctgcgc gcgccgcgca ggcgtcgacg atgagcgggc cagtgcgcac gggcgcgccg
       61 gcggtcgcgc tcagcgaccg cgggcagcgt cgtcgcctcg agatcctcga cgcggcgctg
      121 cgcatcatgg cgcgcgacgg gctgcgggcg gtcagtcatc gtgccgtcgc cgccgaggcc
      181 ggcgtgccgc tcgcggccac cacctactac ttcagcgacc tcgaggacct catcacggag
      241 gcgttcctgc actggtccgg gtcgcagctc agggtcgtgg gcgaattcca cgccgccgcg
      301 ctcgacgtcg tcaggcgggc cggcggcgag cgcgccgggc aggcgggcct ggtcgatgcg
      361 ctggcggacg ccgcgtccga ctacgtcgtg gaccaggtgc tgaaccaccg gccggatcgc
      421 gtgctcgagt tcgcgttcct gcacgaggca gcgcggctgc cgcgactgcg ttcggcggtg
      481 cgcgagcgcc agatgcagga ccgccgcttc ctcgagcagt tccacgcggc ggccggctcg
      541 cccgacccgg tcctcgatgc ccagatttcc ttcagcctgc tgctcgggct ggagaaggcg
      601 gcgctgctgg cggacccgga cgcgctcgac cgtgagtggg tgcgcgaggt gctggtgcac
      661 tacctgtgca atgcgctgag cggcggtcac cggggctgaa cgaagcgttg acggggggaa
      721 ggccgaaatg gtacaaacgt accagttaga aagcgcccgc aaggcgctct tttcgtttca
      781 cggggtccct cgatgaatgc aggccacgtc gcgcggctgc tgctgtgcgt gaccatgttg
      841 tccgcggcgc catcggcggg tgcggccagc ggcaaggaca tcttcgccgc gacctgcgtc
      901 gcgtgccacg gcgcgaatgc gcagggcaat ccggcggttg gcgcgccggc actcgcggga
      961 caggcagcgg cctacctcga gcggcaactc ggcgactttc gcacggggct gcgcggcgcc
     1021 gatccgaagg acacgtacgg cagccagatg cgcgccatcg ccgcgacact cacggacccg
     1081 gccgcggtga aagccgtggc cgcgtatctc gcatcgctgc ctccgccgat cgtcaagccg
     1141 gcggctggcg ccaacctgcg caacggcaac aacgtctacc agggcaagtg cggtgcctgc
     1201 cacggcgggc gcgcggaagg catcgcggcg atgtccacnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnngg catggtgatg aaccaggatg cagcggcgag tctcatcacg
     1381 ctgagcgaac gctgcccgcc gagtttcgag aagaccgcgc agggcacctg cgagctgcgc
     1441 gatctctacc agttctacga ttcgctggaa ggcgcgggcc tcggtggaac ccagaccggc
     1501 ctgccgccga atcgcgacgg cttcaccccg gccgagatcg acctcggccg ctatctgttc
     1561 ttcgacccgc tgctctcagg cgacggcacg ctctcgtgcg cgagctgcca ccggccagaa
     1621 cagggcttga gcgacgggcg cccgcgctcg atcggcgtgc acgggcagga cgtcggacgc
     1681 gcggcaccga cgctgtggaa tgtcgcgttc ctgaagagct tcttctggga cgcgagggcc
     1741 ggctcgctcg aagcgcaggc cgtgggcccg ctcttctcgt cgaaggagat gggcaacacg
     1801 cccgagcggc tgctcgccgg gctgaacggc aatgccgact accggcgcct gttcgccgac
     1861 gcattcccgg cactgcgtgg caagccgatc gcactcccgc aggtcgagcg cgcgctcgcg
     1921 gcattccagt cgtcgctcat ctcgctcaac agccgctacg accgctatgc gcagggctac
     1981 cacgccgcgc tcagcgagca cgagatcgag ggtctgaacg tattccgctc gttcgtcgcc
     2041 cgctgcgccg agtgccacac gccgccgctg ttcacgaacc agcaggtcgc ggtgatcggg
     2101 gcgccggaac cgaagggccg cccgttcgac gtcggtgccg aggccacgtt ccacgccgcg
     2161 aaattgaagg gcggattcaa ggtgccgacg ctacgcaaca tcgcgaggac cgcgccctac
     2221 atgcactcgg gagcgttcgg cacgctgcgc gacaccgttg tcttctacaa caagggccgc
     2281 ggcaacgccg tgccgaaggg gatgacgctg tacctgcact ggcacatcgg gtccccgcac
     2341 ctcaccgact acgaggtgga ccgcctggtg gacttcatgc aggcactcac cgacgagacc
     2401 ttcatgccgg agatcccggc acgcgtgcct tcgggcctgc gtccgatcga caacccgccg
     2461 caaggcgccg cggccacccg cactgcaatg gcgcaagaaa agaacacacg aggagaattc
     2521 cgatgaagaa gggtctggta ctggccgtcg tcgccgccat cgccgcgttc gcgggcggcg
     2581 cgtggtacgg ccgcacgtcg aagtccgcga tggtcgtcac ctcgggcagc ggcggcgcga
     2641 gctacaccgg tggcttcaag gccgacagcg ccgcggcagc ggcggagaag gccggcacga
     2701 tccgctcggt cgccggtccc gcgccgcaga aggaagccat cgtcaaggac ggcgagtcca
     2761 tccaggccgc cgtgaaggcc gcgtccgcgg gcaccatcat ccgcgtgatg cccggcacgt
     2821 atcacgagac cgtctacgtg gacaaggacg acatccgcat catcggcgtg atcgacgccg
     2881 gccggcgccc ggtgctcgac ggcgagggca agctgaacga cgccgtcctg tactccggca
     2941 acaacttcgt ggtcgagaac ctgaccatcc gcaactacaa gggcaacggc atcatgggcc
     3001 aggccggcaa caacttcgag atccgc
//
LOCUS       DAOMFD010000229         3021 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16722_length_3021_cov_36.899528, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000229 DAOMFD010000000
VERSION     DAOMFD010000229.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3021)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3021
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16722_length_3021_cov_36.899528"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1079)
                     /locus_tag="PL152_10145"
     CDS             complement(<1..1079)
                     /locus_tag="PL152_10145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HQR49688.1"
                     /translation="MPTPDRPEVVTAAGESLESRLPARRLTADGKAHVDYGAIGALAW
                     PLMLNSSLQAAINLTDTWFVGHISTAAMAGMAAIFWLVFFFVMLLGGIGLAVQTFAAQ
                     YEGGRRRWRAGHATWVALWGAVATLPVFAVIGWGGDLLLAPFGLDPEVERNALAFWQP
                     RMLGAPLGVGLWALLGFFNGVSRPRITVLVTLIVGVVNAILNEVFIFRLGWGIAGSAW
                     ATNVSMGIGLGVALAIFLRADLRRAYRTHLTWRPDLASLWRDLRLGLPMGAMYAADLF
                     GMALFSLMQVRLGQVDGATTQIVVMLTSIAYLPGVGLALAGTTLVGQAIGAGDRDWAR
                     RLGNTVIAITMGYMGSVGVLLALLGP"
     gene            complement(1066..1707)
                     /locus_tag="PL152_10150"
     CDS             complement(1066..1707)
                     /locus_tag="PL152_10150"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HQR49689.1"
                     /translation="MRDFIATLMHWYLGALDQGGYLLIALLMAAESSILPVPSELVIP
                     PAAYLAHAHGTFSYPGIVMAGTIGSWVGATVMYWVARLLGRPLLMRYGRYVMITPEKI
                     EHAENWSAHYGGMGVFVARILPVIRHLIGIPAGIVRLNYWKFSLATVAGSALWCSVLV
                     WLGVTAGQDKLLLEGSLHRISLWGGGVLVFVWGLYYILVHRQMRRRGERRADP"
     gene            1871..>3021
                     /locus_tag="PL152_10155"
     CDS             1871..>3021
                     /locus_tag="PL152_10155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49690.1"
                     /translation="MHRLYASFACLIVLLLPVQYAAGAGAAATPSKDTGGAAKPAAPE
                     VIGAAEIPLRSDIDERYVQEVIARARQPDPSMKLAGRLDEMATGIKKLSETFKRDELL
                     SLPAMRLESLERHWNFYDQQLADWRAELQRGTARYSEDAAELAKRRAAWEATRTAAEG
                     SGLTPALAERVDSMLAEIGDAEQVLSVPLDNQFRLARRGNSVQQAVDAGQKAVDAAIK
                     NFDRRLGVIDAPPIWEAWRTGGASGRELSTALVGLGIETQFLDEYNARHAKSLKLLTA
                     AALVLLPLLIWLSRRSRKIVANDPELESSARVLLRPISSWLVLVLVGILFLEPNAPII
                     RHEAALLFALIPVLRLLPSKVYQVLGSWPYVVTGLYLLDQLAFLFVGVPL"
BASE COUNT          498 a         1062 c         1046 g          415 t
ORIGIN      
        1 ggcccgagca gcgcgagcag cacgccgacc gagcccatgt aacccatcgt gatcgcgatc
       61 accgtgttgc cgagccggcg cgcccagtcg cggtcgcccg cgccgattgc ctggccgacc
      121 agcgtcgtcc cggccagcgc aaggccaacg cccggcaggt atgcgatcga ggtgagcatc
      181 acgacgatct gcgtcgtcgc gccgtcgacc tgcccgagcc gcacctgcat cagcgagaac
      241 agcgccatgc cgaacaggtc cgccgcgtac atcgcgccca tcggcaggcc gaggcgcagg
      301 tcccgccaga ggctcgcgag atcgggccgc cacgtgaggt gcgtgcggta ggcccgcctt
      361 aggtcggcgc gcaggaagat cgcgagcgcg acgccgaggc cgatccccat gctgacgttc
      421 gtcgcccagg ccgagccagc gatgccccaa cccaggcgga agatgaagac ctcgttcagg
      481 atcgcattga cgacgccgac gatcagcgtg accagcacgg ttatgcgggg ccgcgacacg
      541 ccattgaaaa atccgagcaa cgcccagagt cccacgccga gcggggcgcc cagcatgcgg
      601 ggctgccaga acgcgagcgc gttgcgctcg acctccgggt cgaggccgaa cggtgcgagc
      661 agcaggtcac cgccccaacc gatcaccgcg aagaccggca gcgtggccac cgcgccccac
      721 agtgcgaccc aggtcgcgtg cccggcgcgc cagcggcgcc gacctccctc gtactgcgcg
      781 gcgaaggtct gcaccgcgag cccgatgccg ccgagcagca tcacgaagaa gaacacgagc
      841 cagaagatgg cggccatgcc cgccatcgcc gcggtcgaga tgtgccccac gaaccatgtg
      901 tcggtgaggt tgatggccgc ctgcaggctg ctgttcagca tgagcggcca ggcgagcgca
      961 ccgatcgccc cgtagtccac atgggccttg ccgtcggccg tcaggcgccg tgccggcagc
     1021 cgcgactcga ggctctcgcc cgctgcggtg accacttcgg gccggtcagg ggtcggcacg
     1081 tcgctcgccc cggcgccgca tctgccgatg cacgaggatg tagtacaggc cccagacgaa
     1141 caccagcacg ccgccgcccc acagcgagat gcggtgcagg ctgccctcga gcagcagctt
     1201 gtcctggccg gcggtcacgc cgagccacac cagcacgctg caccacagcg ccgatcccgc
     1261 gacggtcgcg agcgagaact tccagtagtt caggcgcacg atgccggcgg ggatgccgat
     1321 caggtgccgg atcaccggca ggatgcgcgc cacgaacacg cccatgccgc cgtagtgcgc
     1381 ggaccagttc tcggcgtgct cgatcttctc gggcgtgatc atgacgtagc gcccgtagcg
     1441 catgagcagc ggccggccga gcaggcgcgc gacccagtac atcacggtgg cgcccaccca
     1501 cgaaccgatc gtgcccgcca tgacgatgcc tgggtacgaa aacgtgccgt gcgcgtgcgc
     1561 gaggtaggcc gccggcggga tgaccagctc gctcggcacg ggcaggatcg agctttccgc
     1621 cgccatgagc agcgcgatga gcaggtaccc cccctggtcc agggcaccga ggtaccagtg
     1681 catcaacgtt gcgatgaaat cacgcatgct gggggcgggt ccgacctggc gcgcggcgga
     1741 cgggcctgac cggcgcggca gggcattctg ccagatcgcg gggcacaacc cgcggaatgg
     1801 tcgcggtgcc gaacgcgcgc cgctgatgcg acaatggctg ctgccccgta cctgggctgg
     1861 aacggtccct atgcatcgcc tgtacgcgtc cttcgcctgc ctgatcgtcc tgctgcttcc
     1921 cgtccagtac gcggctggcg caggggccgc ggcaaccccg tccaaggaca cggggggagc
     1981 cgcgaagcct gcggccccgg aggtcatcgg cgccgcggag atcccgctgc gatcggacat
     2041 cgacgagcgc tacgtgcagg aggtcatcgc gcgggcgagg cagcccgacc cttcgatgaa
     2101 gctcgccggg cggctggatg agatggccac cggcatcaag aagctctcgg agaccttcaa
     2161 gcgcgacgag ctgttgtcgc tgccggccat gcggctcgag agtctcgagc ggcactggaa
     2221 tttctacgac cagcagctcg ccgactggcg cgccgaactg caacgcggca cggcccgtta
     2281 ctccgaggac gccgccgaac tcgcaaagcg gcgggctgct tgggaggcga cccgcacggc
     2341 ggccgagggc agcggcctca cgccggcgct ggcggagcgc gtcgactcga tgctggccga
     2401 gatcggcgac gcggagcagg tcctgtcggt cccgctcgac aaccagttcc gcctcgcgcg
     2461 acgcgggaac tcggtgcagc aggccgtcga cgccggccag aaggcggtgg acgctgcgat
     2521 caagaacttc gatcgccggt tgggcgtgat cgacgcgccg ccgatctggg aggcgtggcg
     2581 cactggtggc gcctccgggc gcgagttgtc gaccgcactc gtagggctgg ggatcgaaac
     2641 ccagttcctg gacgagtaca acgcccgcca cgcgaagagc ctgaagctgc tgactgcggc
     2701 ggcgctggtc ttgctgccgc tgctgatctg gctgagccgg cgcagccgga agatcgtggc
     2761 gaacgacccc gaactcgaat cgtccgcgcg ggtgctcctg cggccgattt cgtcgtggtt
     2821 agtgctggtc ctggtcggga tcctgttcct cgagcccaat gcgccgatca tccggcacga
     2881 ggccgcgctg ctgttcgcgc tcatcccggt gctgcggctg ctgccgagca aggtctacca
     2941 ggtgctgggc tcgtggccgt acgtcgtcac ggggctctac ctgctcgacc agctggcatt
     3001 cctgttcgtg ggcgtgccgc t
//
LOCUS       DAOMFD010000230         3020 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16733_length_3020_cov_25.861046, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000230 DAOMFD010000000
VERSION     DAOMFD010000230.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3020)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3020
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16733_length_3020_cov_25.861046"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="PL152_10160"
     CDS             <1..389
                     /locus_tag="PL152_10160"
                     /inference="COORDINATES: protein motif:HMM:TIGR02481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HQR49691.1"
                     /translation="IVSIDDDHHRMVAALNGIHDLIAGSAGAAAIAEALGEFHTAAAA
                     HFALEERILQEERHPDFPARRQIHYRLLDQVRDVMDAYEAGAYGAGEQLPTTLRQWLT
                     EAIGMDARLFAQLNEAGLQRWGLKRG"
     gene            complement(448..521)
                     /locus_tag="PL152_10165"
     tRNA            complement(448..521)
                     /locus_tag="PL152_10165"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(487..489),aa:Cys,seq:gca)
     gene            complement(589..664)
                     /locus_tag="PL152_10170"
     tRNA            complement(589..664)
                     /locus_tag="PL152_10170"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(629..631),aa:Gly,seq:gcc)
     gene            complement(728..1297)
                     /gene="pgsA"
                     /locus_tag="PL152_10175"
     CDS             complement(728..1297)
                     /gene="pgsA"
                     /locus_tag="PL152_10175"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HQR49692.1"
                     /translation="MKIEDLPNVLTVGRIVAVPLVVVLFYWANPWSDPLAALVFIAAA
                     ITDSLDGWLARKMGITTPLGEFLDPVADKLMVATALVLLVGHDTRPLIVITAVVIIGR
                     EITVSALREWMAHLGARAKVAVSGMGKLKTIVQMTGLSMMMFRRDVLGLPVYEIGVVL
                     LVLAAALTLWSMGSYLRAAWPELRREHVA"
     gene            complement(1294..>3020)
                     /gene="uvrC"
                     /locus_tag="PL152_10180"
     CDS             complement(1294..>3020)
                     /gene="uvrC"
                     /locus_tag="PL152_10180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HQR49693.1"
                     /translation="KARHLRNRVSSYFKGHAHGERTQAMLGQVARVEVTVTASEIEAL
                     LLEDNLIKRHRPRYNVLLKDDKSFPFIRITAHEFPRLEFYRGSRKEAGRYFGPYPSTV
                     ATRETLLLLQKLFRLRPCEDTFFANRSRPCLQYQIQRCTAPCVGLVSQDTYSRDVADA
                     ARVLEGRNDEVVTDLRRRMDLASGELRFEEAARLRDQVAMLKQVQASQSATRARGTDV
                     DAVAVASAGQDCCVSVVFVRGGRNLGSSNFHTKGGLGGAAEALRAFVAQHYLAREAPP
                     EILLGGAVDDAELLETALAERSGHAVRIRTAVRGTRARWVAMARANAELGLTMKAASR
                     AGLEEQFESLGTELGLGRAPTRIECFDVSHTMGESAVASCVVMGAEGPMKSSYRRFNL
                     RDLTPGDDYGGLRQAVERHYTRIAKGEMPAPDVLLIDGGPGQLAAAMEALDVTGVAPE
                     IVVGVAKGADRRPGQERLFLAGQEAPLILQPGSPALRLIQRVRDEAHRFAITGHRKAR
                     DLARRESFLEEIPGLGPARRRELLKAFGGLQGVKQASVEDLAKVQGISRKLAQRIYER
                     MNPGPERA"
BASE COUNT          488 a         1060 c         1006 g          466 t
ORIGIN      
        1 gcatcgtcag catcgacgac gaccaccacc ggatggtggc ggcgctgaat ggcattcatg
       61 acttgatcgc gggcagcgcc ggggccgcgg caatcgccga agccctcggg gaattccata
      121 ccgcggcggc agcgcatttt gcgctcgagg aacggatcct tcaggaggaa cgtcacccgg
      181 acttccctgc gcgacgacag atccactacc gcctgctcga ccaggtgcgc gatgtcatgg
      241 acgcatacga agcgggcgcc tacggcgccg gcgagcaact gccgacgacg cttcggcagt
      301 ggctcaccga ggccatcggt atggatgcca ggctgttcgc acagttgaac gaggcgggat
      361 tgcagcgctg gggcctcaag cggggctgag ccagcgcgcg aggtccgccg gcacccgttg
      421 aacggggaac tggtgcggac ctgtcgatgg aggctagggt cggaatcgaa ccggcgtaca
      481 cggctttgca ggccgctgca tgaccactct gccacctagc cgcgatggcg acactgcgcc
      541 cgccagaaac gcgtgacccc ggggctggcc cggggtcacg tgagaaactg gagcgggaaa
      601 cgagactcga actcgcgacc ccgaccttgg caaggtcgtg ctctaccaac tgagctattc
      661 ccgcccgggc atcccggcga ccctggtcgc cgggggacgc gaattttcaa agggttaggt
      721 ccaactgtca agcgacgtgc tcacgccgca gttccggcca ggccgccctc aggtagctgc
      781 ccatggacca caacgtgagc gcggccgcca gcaccagcag caccacgccg atctcgtaga
      841 caggcaggcc gaggacgtcg cgccggaaca tcatcatgct gaggcccgtc atctgcacga
      901 tcgtcttgag cttgcccatg ccggatacgg ccaccttggc gcgcgcgccg aggtgcgcca
      961 tccactcgcg cagcgccgag acggtgatct cgcgaccgat gatcacgacg gccgtgatca
     1021 cgatgagcgg ccgggtgtcg tgacccacca gcagcacgag cgcggtggca accatcagct
     1081 tgtccgccac gggatccagg aactcgccca ggggcgtcgt gatgcccatc ttcctcgcga
     1141 gccagccgtc cagcgagtcg gtgatcgccg cggcgatgaa gaccagcgcg gccagcgggt
     1201 cggaccacgg gttggcccag tagaacagca cgacgaccag gggcaccgcg acgatgcggc
     1261 cgacggtgag gacgttgggc aggtcttcga ttttcacgcg cgctccggcc ccgggttcat
     1321 ccgttcgtag atcctctggg cgagcttcct gctgatgccc tgcaccttgg ccaggtcctc
     1381 gacggacgcc tgcttcacgc cctgcagccc gccgaaggcc ttgagcaact cccggcgccg
     1441 ggccgggccg agaccgggaa tctcctcgag gaacgactcg cgccgcgcga ggtcgcgcgc
     1501 cttgcggtgc ccggtgatcg cgaagcggtg cgcctcgtca cgcacccgct ggatcaaccg
     1561 cagcgccggt gaacccggct gaagtataag cggggcctcc tgaccggcca agaacaggcg
     1621 ttcctggcct gggcgtcggt cggcgccttt tgcgaccccg accacgattt caggcgcgac
     1681 tccagtgacg tcgagcgcct ccatcgccgc ggcgagttgt ccgggtccgc catcgatcag
     1741 cagcacgtcc ggcgccggca tctcgccctt cgcgattcgc gtgtagtgtc gctccacggc
     1801 ctggcgcagc ccaccgtagt cgtcacccgg agtcaggtcg cgaagattga agcggcggta
     1861 cgaactcttc atgggtccct ccgctcccat caccacgcac gacgcgaccg cggactcccc
     1921 catcgtgtgg ctgacgtcga agcactcgat gcgtgtcggc gcgcggccga gtccgagttc
     1981 cgttcccagc gactcgaact gctcctcgag gccggcgcgg ctcgcggcct tcatcgtgag
     2041 ccccagttcc gcattggccc gggccatcgc cacccagcgt gcgcgcgtgc cgcgcaccgc
     2101 ggtccggatc ctcaccgcgt ggccactgcg ctccgcgagc gcggtctcga gcagttcggc
     2161 atcgtccacc gccccgccga gcaggatctc gggcggggcc tcgcgggcca ggtaatgctg
     2221 cgcgacgaat gcgcggaggg cctcggctgc gccgcccagt ccgcccttgg tgtggaagtt
     2281 gctgctgccg aggttgcgcc cgcccctcac gaagacgacc gacacgcaac agtcctgccc
     2341 ggcgctcgcg accgcgacgg catcgacatc cgtaccgcgc gctcgcgtgg ccgactggct
     2401 ggcctggacc tgcttcagca tcgcgacctg gtcccgcagc ctcgcggcct cctcgaaccg
     2461 gagttcgccc gaggccaggt ccatgcgccg gcgcaggtcg gtcaccactt cgtcattgcg
     2521 accctccagc acgcgggcgg cgtcggcgac gtcgcgcgag taagtgtcct gcgacacgag
     2581 ccccacgcac ggtgccgtgc agcgctggat ctggtactgg aggcacggcc gggaacgatt
     2641 ggcgaagaac gtgtcctcgc agggccgcag ccggaagagt ttctgcagca gcagcagcgt
     2701 ctcgcgcgtg gcgaccgtgc ttggatacgg gccgaagtac cggccggcct ccttgcgcga
     2761 cccgcggtag aactcgagcc gcgggaactc gtgggccgtg atgcggatga acgggaagct
     2821 cttgtcgtcc ttgagcagga cgttgtaacg cggccggtgc cgcttgatca ggttgtcctc
     2881 gagcagcagt gcctcgatct ccgaggccgt caccgtcacc tcgacgcgag ccacctggcc
     2941 gagcatcgcc tgggtgcgct cgccatgcgc gtgccccttg aaatagctgg agacgcgatt
     3001 gcggaggtgc cgcgccttgc
//
LOCUS       DAOMFD010000231         3016 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16768_length_3016_cov_33.527187, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000231 DAOMFD010000000
VERSION     DAOMFD010000231.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3016)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3016
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16768_length_3016_cov_33.527187"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /gene="cysG"
                     /locus_tag="PL152_10185"
     CDS             complement(<1..1070)
                     /gene="cysG"
                     /locus_tag="PL152_10185"
                     /EC_number="1.3.1.76"
                     /EC_number="2.1.1.107"
                     /EC_number="4.99.1.4"
                     /inference="COORDINATES: protein motif:HMM:NF007922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0043115 - precorrin-2 dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051266 - sirohydrochlorin ferrochelatase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="siroheme synthase CysG"
                     /protein_id="HQR49694.1"
                     /translation="MKYFPMFADLQERRVLVVGGGEVAERKVRLLLEAGAQVEVVAPA
                     LGDSLQGRLAESPGRVAWIAPRFDEAQLQGVALVIAATSDAAVNSQVAAAARARGLFV
                     NVVDDAGRSGFIVPAIVDRSPLVIAVSSGGAAPVLARLVRERIEVVVDGSVGALARLL
                     ERWRARIKAAIPDVTRRRRFYEATLRGDVAAALRGAQPEAAERALAQALARGVTAAGS
                     VALVGAGPGDPGLLTLNALRALQDADVVLYDRLVSSEVLGLARRDATCIEVGKRGRGR
                     SVPQDRIHELMLEHARQGRRVVRLKGGDPFIFGRGGEELEFLAAHRVPYEVVPGITAA
                     VACGAYAGVPLTHREHAQSVRFV"
     gene            complement(1075..1806)
                     /locus_tag="PL152_10190"
     CDS             complement(1075..1806)
                     /locus_tag="PL152_10190"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: protein motif:HMM:NF002537.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HQR49695.1"
                     /translation="MGLRDFEAEAARVEPKLLDAANDALGRMHASQRVEWALEHLPGH
                     HALSSSFGAQAAVSLHLVTRAAPQIPVILVDTGYLFAETYRFVDEIVMRLGLNLKVYQ
                     PRVSPAWLEARHGKLWEQGVDGIERYNDLRKGEPMLRALDELGVASWFAGLRRYQSRT
                     RSSVRPLEYRSGRWKVHPIVDWTDRQVFQYLTRHDLPYHPLWHQGYVSIGDWHTTRTL
                     AEAGDAEATRFFGLKRECGIHGLEG"
     gene            complement(1793..>3016)
                     /locus_tag="PL152_10195"
     CDS             complement(1793..>3016)
                     /locus_tag="PL152_10195"
                     /inference="COORDINATES: protein motif:HMM:NF010029.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004783 - sulfite reductase (NADPH)
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     hemoprotein subunit"
                     /protein_id="HQR49696.1"
                     /translation="RAHAAAHEWAVKLSEHLLPRTRAYHEIWLDGEKVAGTPEVEPIY
                     GPTYLPRKFKAAIAVPPVNDVDVFAQDLGFVAIVEDGELAGFNVTVGGGLGATHGEAA
                     TYPRLADVVGFVKPEQLLAVAEAVVTTQRDFGDRANRKHARLKYTIDERGLDWFVAEI
                     TRRQGFALEAARPFEFTSNGDRFGWTEGHDGRWHLTLRIEAGRVADTAAGKHLTGLRE
                     IARIHRGDLRLTPNQNLIVANVAPSERRFIDALVVRYGLDAHARRTPLRRDALACVAL
                     PTCPLAMAEAERYLPDFGRKVERLLATHGLRDEPVTLRITGCPNGCARPYLAEIALIG
                     KAPGRYNLHLGGDGRGMRLNALRLENVDEAAILGDLDGAFARYAAERQPGERFGDYAW
                     RAGLVGVAGDTRGPA"
BASE COUNT          443 a         1111 c         1030 g          432 t
ORIGIN      
        1 acgaaccgca cggactgcgc gtgctcgcgg tgcgtgagcg gcacacccgc atacgcgccg
       61 caggccactg ccgccgtgat gcccggcacg acttcgtacg gcacgcggtg ggccgcaagg
      121 aattccagct cctcgccgcc gcgcccgaag atgaacgggt ccccgccctt cagccgcacc
      181 acgcgccgtc cttgccgggc atgctcgagc atgagttcgt ggatgcggtc ctgcggcacg
      241 gaccggccgc gtccccgctt accgacctcg atgcaggtcg cgtcgcgccg ggcgaggcca
      301 agcacctcgc tcgagacgag ccggtcgtac aggacgacgt ccgcgtcctg cagcgcccgg
      361 agcgcattga gcgtgagcag gccgggatcg cccgggccgg cgccgaccag ggccaccgag
      421 cccgccgcgg tcacgcctcg cgccagcgcc tgcgcaagag cgcgctcggc ggcttccggc
      481 tgcgcgccgc ggagggcggc ggccacgtcg ccgcgcaagg tggcctcgta gaagcgccgg
      541 cgacgcgtca cgtcgggaat cgccgccttg atccgcgcgc gccagcgttc cagcagtcgc
      601 gcgagggcgc cgaccgaacc gtccacgacg acttcgatgc gctcgcgcac gaggcgtgca
      661 aggaccggcg ccgcgccgcc gctcgacacg gcgatcacta gtggcgaccg gtccacgatc
      721 gcgggcacga tgaaccctga gcgccccgcg tcgtccacca cgttgacgaa taggccgcgg
      781 gcacgtgccg cagccgcaac ctgcgaattc accgcggcat cgctggtcgc tgcgatgacg
      841 agcgcgacac cctgcagctg cgcctcgtcg aaccgcggcg cgatccacgc cacccgaccc
      901 ggagattcgg ccagcctgcc ttgcagcgaa tcgccgaggg ccggagcgac cacctcgacc
      961 tgggcgccgg cctcgagcaa caggcggacc ttgcgctcgg ccacctcgcc gccgccgacc
     1021 acgaggacgc ggcgctcctg caggtcggcg aacatgggga agtacttcac ccggtcagcc
     1081 ctcgagcccg tggatgccgc actcgcgctt gaggccgaag aagcgcgtcg cttcggcgtc
     1141 cccggcttcg gcgagcgtgc gcgtcgtgtg ccagtcgccg atggacacgt agccctggtg
     1201 ccacagcggg tggtacggga gatcgtggcg ggtcaggtac tggaacacct gccggtcggt
     1261 ccagtccacg atcgggtgga ccttccaccg tccactgcgg tactcgagcg gacggacgga
     1321 actacgggtg cgcgactggt atcgacgcaa tcccgcgaac cagctcgcga caccgagttc
     1381 gtcgagcgcg cgcagcatgg gctcgccctt gcgcaggtcg ttgtagcgtt cgatgccgtc
     1441 gacgccctgc tcccatagct tgccgtgtcg cgcctcgagc caggccggcg agacgcgggg
     1501 ctggtacacc ttgaggttca gcccgaggcg catgacgatt tcgtcgacga accgataggt
     1561 ctcggcgaac aggtagcccg tgtcgaccag gatgaccggg atctgcggcg ccgcgcgagt
     1621 gacgagatgc aacgacacgg ccgcctgcgc gccgaaactc gacgagagcg cgtgatggcc
     1681 cggcaggtgc tccagcgccc actcgacgcg ctggctcgcg tgcatgcgtc cgagcgcgtc
     1741 gttcgccgca tcgagtaact tcggctcgac gcgcgcggcc tcggcttcga agtcacgcag
     1801 gcccacgggt gtctcctgcc acgccgacga gccctgcccg ccacgcatag tcgccgaagc
     1861 gctcgcccgg ctggcgttcc gccgcgtagc gcgcgaaggc cccgtccagg tcacccagga
     1921 tcgctgcctc gtcgacgttc tcgaggcgca acgcgttcag ccgcatgccg cgaccgtcgc
     1981 cgccgaggtg caggttgtag cgtcccggcg ccttgccgat cagcgcgatc tccgcgaggt
     2041 acggccgtgc gcagccgttc gggcaccccg tgatgcgcag cgtgaccggc tcgtcgcgca
     2101 gtccgtgcgt ggccagcagc cgctccacct tgcgcccgaa gtccggcagg tagcgctccg
     2161 cctctgccat cgccagcgga caggtcggca gcgcgacgca ggcgagcgca tcgcggcgca
     2221 gcggggtgcg ccgtgcatgc gcgtccagcc cgtaacgcac gaccagcgcg tcgatgaagc
     2281 ggcgctccga tggcgcgacg ttagcgacga tcaggttctg gttcggcgtc aggcggagat
     2341 ccccgcgatg gatgcgcgca atctcgcgca gccccgtcag gtgctttccg gccgccgtgt
     2401 ccgcgacgcg cccggcctcg atacgcagcg tcaggtgcca gcgtccgtcg tgtccctccg
     2461 tccatccgaa gcggtcgcca ttcgacgtga attcgaacgg ccgcgcagct tcgagcgcga
     2521 agccctgccg ccgggtgatc tccgccacga accagtcgag tccgcgctcg tcgatcgtgt
     2581 acttgaggcg cgcgtgcttg cggttcgccc ggtcgccgaa gtcgcgctgc gtcgtcacca
     2641 cggcttcggc cacggcgagc aactgctccg gcttcacgaa gcccacgacg tctgcaagcc
     2701 gcgggtacgt cgccgcttcg ccgtgcgtgg cgccgagacc cccgccgacg gtgacgttga
     2761 agccagcgag ttccccgtcc tcgacgatcg cgacgaatcc gaggtcctgc gcgaacacgt
     2821 ccacgtcgtt gacgggcggc acggcgatcg cggccttgaa cttgcgtggc aggtacgtcg
     2881 gaccgtagat cggctcgact tccggcgtgc ccgcgacttt ctcgccgtcc agccagatct
     2941 cgtggtaggc gcgcgtgcgc ggcagcaggt gctccgacag cttgacggcc cattcatgcg
     3001 ccgcggcatg ggcgcg
//
LOCUS       DAOMFD010000232         3005 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16881_length_3005_cov_30.234237, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000232 DAOMFD010000000
VERSION     DAOMFD010000232.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3005)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3005
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16881_length_3005_cov_30.234237"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /gene="pheS"
                     /locus_tag="PL152_10200"
     CDS             <1..399
                     /gene="pheS"
                     /locus_tag="PL152_10200"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004979194.1"
                     /note="catalyzes a two-step reaction, first charging a
                     phenylalanine molecule by linking its carboxyl group to
                     the alpha-phosphate of ATP, followed by transfer of the
                     aminoacyl-adenylate to its tRNA; forms a heterotetramer of
                     alpha(2)beta(2); binds two magnesium ions per tetramer;
                     type 1 subfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HQR49697.1"
                     /translation="HQVEGLVVDEGVSLAHLKSVLHHFVERFFERRLGIRFRPSYFPF
                     TEPSAEVDIECVFCSGQGCRVCKTTGWLEVAGCGMVHPNVLSNVGIDPERYTGYAFGM
                     GIERLTMLRYNVNDIRMYFDNDLRFLRQFG"
     gene            418..2796
                     /gene="pheT"
                     /locus_tag="PL152_10205"
     CDS             418..2796
                     /gene="pheT"
                     /locus_tag="PL152_10205"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HQR49698.1"
                     /translation="MRVSIDWLSEWLGTKPEPHELAAGLTMAGLEVEAVEPAAPPLPG
                     VIVGEILERAKHPDADSLSVCKVSTGAEVVQVVCGAPNARAGMKAPLATIGAKLPGGM
                     EIRKARLRGVESFGMLCSARELGLSEDAAGLMELPAEAPTGAALSDALSLADTILEVN
                     LTPNRGDCMGMLGIAREAALLTGATLGGPAAVAVPPQSQDRFPVELEAGAGCARFVAR
                     VVRGVRADAQSPLWMRERLRRAGLRPISAIVDVTNYVMLELGQPMHAYDLRELEGGIV
                     VRRARAGETLRLLDGRDVTLDESVLVIADRAKPLGLAGIMGGEHSGISATTTDVLLEV
                     AFFLPDAVAGRGRRYGLVTDASQRFERGVDPTLQERAIERATQLLLGCAGGVPGPTAV
                     AERTEDLPPRRSISFRPDRARRVIGAPVSDHAMAGIFDKLGMAVSRTAAAWQVTVPPW
                     RFDLGIEEDLIEEIARVHGFDEIPETDASVRESMPSLTERRVASEAAADMLVQRGYHE
                     AITYSFVDPAVQTLFCPDEAALALANPIAADLAVMRVSLWPGLAKALVENQRRQQSRV
                     RLFEIGRRFNQPGGRLTEVSVIAGIAAGTALPEQWGTRAEPVDFFDVKADVEALLSAT
                     GARGDFRFVPAQHVALHPGQAARILRDDRPVGWLGRLHPEIESRLDLTYSALVFEIET
                     ESGLAAVVPQHQEISRFPAVRRDIAVIVEESVPVDALLDRIRASAGALLTDLVVFDIY
                     RGAGITSGRKSIAIGLNLQDISRTLTDVDTDAVVAQVVRDLEREHGATIRDK"
     gene            2797..>3005
                     /locus_tag="PL152_10210"
     CDS             2797..>3005
                     /locus_tag="PL152_10210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012613949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HQR49699.1"
                     /translation="MALTKAEIAEALFDQLGLNKREARELVDLFFEELRASLATGEQV
                     KLSGFGNFDLRSKNQRPGRNPKTGE"
BASE COUNT          477 a          970 c         1076 g          482 t
ORIGIN      
        1 caccaggtcg aaggcctggt ggtggacgag ggcgtgagcc tcgcgcacct gaagtccgtg
       61 ctgcaccact tcgtcgagcg gttcttcgag cgccggctcg ggatccgctt ccggccctcg
      121 tacttcccgt tcacggagcc gtcggccgag gtggacatcg agtgcgtgtt ctgcagcggc
      181 cagggctgcc gcgtgtgcaa gacgaccggc tggctcgagg tcgcgggctg cgggatggtt
      241 cacccgaacg tgctcagcaa cgtcggcatc gacccggagc gctacacggg ctacgcgttc
      301 ggcatgggca tcgagcgcct cacgatgctg cgctacaacg tcaacgacat ccgcatgtac
      361 ttcgacaacg acctgcgctt cctgcgccag ttcggttgaa cgggcaggag cacggacatg
      421 cgcgtcagca tcgactggtt gagtgaatgg ctgggcacga agccggagcc ccatgaactc
      481 gcggcagggt tgaccatggc cggcctcgaa gtcgaggcgg tggaaccggc cgcgccgccg
      541 ctgccgggcg tcatcgtcgg cgaaatcctc gagcgtgcga agcacccgga tgcggacagc
      601 ctgagcgtct gcaaggtgag caccggggcg gaggtggtcc aggtcgtctg cggtgcgccc
      661 aacgcccgtg cgggaatgaa ggcgcccctc gcgacgatcg gcgcgaaact ccccggcggg
      721 atggaaatcc gcaaggccag gctgcgcggc gtcgagtcgt tcgggatgct gtgttcggcg
      781 cgcgaactcg gattgtccga ggacgccgcg ggactcatgg agctgccggc ggaggcgccg
      841 accggcgccg cgctgtccga tgcactgtcg ctggccgaca cgatcctcga ggtcaacctg
      901 acgcccaacc gcggcgattg catgggcatg ctcggcatcg cgcgcgaggc ggccctgctc
      961 acgggcgcca cgttgggcgg cccggcggcc gtcgcagtcc cgccgcagtc gcaggaccgc
     1021 ttcccggtgg aactcgaggc aggggcgggt tgcgcacgct tcgtcgcccg ggtcgtgcgg
     1081 ggcgtgcgcg ccgatgccca gtcgccgctg tggatgcgcg agcggctgcg gcgcgccggg
     1141 ctgaggccga tcagcgcgat cgtggacgtc acgaattacg tgatgctgga acttggccag
     1201 ccgatgcacg cgtacgacct gcgcgaactc gagggcggca tcgtcgtcag gcgcgcgcgg
     1261 gccggcgaga cgctgcgact gctcgacggg cgggacgtca cgctggatga atcggtcctt
     1321 gtcatcgccg accgcgccaa gccgctcgga ctcgcgggga tcatgggtgg cgaacattcg
     1381 ggcatctccg ccaccacgac ggacgtgctg ctcgaagtcg ccttcttcct gccggatgcc
     1441 gtcgcggggc gagggcgccg ctacggactc gtcacggacg cgtcgcagcg cttcgagcgc
     1501 ggcgttgacc cgacgctgca ggagcgcgcc atcgagcgtg ccacgcagtt gctgctcgga
     1561 tgcgccggtg gcgtacccgg tcccacggct gtcgccgagc ggaccgagga cttgccgccc
     1621 cggcgctcga tttcattccg tccggatcgg gcgcgccgcg tgattggcgc gccggtttcg
     1681 gaccacgcaa tggcaggcat cttcgacaag ctgggcatgg cggtgagtcg aacagcggcg
     1741 gcgtggcagg tcacggtgcc accgtggcgc ttcgacttgg gcatcgaaga ggacctcatc
     1801 gaggaaatcg cgcgcgtgca cggcttcgac gagattcccg agacggatgc ctccgtgcgc
     1861 gagtccatgc cgtcactgac cgagagacgt gtcgcgagcg aagcggcggc ggacatgctc
     1921 gtgcagcgcg gttatcacga ggcgatcacc tacagcttcg tcgatccagc ggtgcagaca
     1981 ctgttttgtc cggacgaagc ggcgctcgcg ctcgccaatc cgatcgccgc ggacctcgcc
     2041 gtcatgcgcg tgtcgctgtg gcccgggctc gcgaaggcat tggtcgaaaa ccagcggcgc
     2101 cagcagtcgc gcgtgcggtt gttcgagatc gggcgccgct tcaaccagcc gggcggccga
     2161 ctgaccgagg tcagcgtcat cgccggcatc gcggcgggca cggcattgcc ggagcagtgg
     2221 ggcacgcgcg cggagccggt cgatttcttc gacgtgaagg ccgacgtgga agccttgctc
     2281 agcgccacgg gcgctcgcgg ggacttccgg ttcgtgccgg cgcagcacgt cgccctgcat
     2341 ccggggcagg ccgcacggat ccttcgcgat gacaggccgg tcggctggct gggacgtctc
     2401 catccggaga tcgagagccg gctcgatctt acatattctg ccctcgtgtt cgagatcgag
     2461 accgagtccg gattggcggc cgtcgtgccc cagcaccagg agatttcgcg gttcccggcc
     2521 gtacggcgcg acatcgcggt gatcgtggag gagtcggtcc cggtcgacgc gctattggac
     2581 agaatccggg cgagtgccgg ggcgctgctc acggacctgg tcgtgttcga catctaccgc
     2641 ggcgcgggca tcacgtcggg tcgtaaaagc atcgcgatag gcttgaattt acaggatatt
     2701 tcccgcaccc ttaccgacgt tgacacggat gccgtcgtgg cacaggtcgt tcgcgacctg
     2761 gagcgggagc atggcgccac gatcagagat aaataaatgg cattgaccaa ggcggaaatt
     2821 gccgaagcac tgttcgacca gctcgggctc aacaagcgcg aggcgcggga gctcgtggac
     2881 ctgttcttcg aggaactgcg cgcgtcgctc gcgaccggcg agcaggtgaa gctctccggt
     2941 ttcggcaact tcgacctgcg cagcaagaac cagcgcccgg gccgcaatcc gaagaccggc
     3001 gagga
//
LOCUS       DAOMFD010000233         3003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16901_length_3003_cov_24.223541, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000233 DAOMFD010000000
VERSION     DAOMFD010000233.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3003
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16901_length_3003_cov_24.223541"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="PL152_10215"
     CDS             <1..212
                     /locus_tag="PL152_10215"
                     /inference="COORDINATES: protein motif:HMM:NF013440.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GreA/GreB family elongation factor"
                     /protein_id="HQR49700.1"
                     /translation="EDDEGHEIELRIVGPDEIDPARRYVSMDSPLARALMRRQRDEEF
                     TVEVPGGRKTYVVTEIRYEAGDDGS"
     gene            307..1437
                     /locus_tag="PL152_10220"
     CDS             307..1437
                     /locus_tag="PL152_10220"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HQR49701.1"
                     /translation="MRSIAHAGLVAALLASLLSSVASCGSGHDKAATAAEEKVLHVYN
                     WNDYIAPTTIADFETRTGIKVVYDVYDSFEVLETKLLTGHSGYDVVVTSGGPTRKLID
                     AGAIKTLDRSKLRNLANLDPRIVQLQTYYDPGNAHTVPYLLGTTGIGYNPDQVEKALG
                     TRTIDSFAAVFDPAIASKLAKCGITWLDSPADLFQVAFIYLGLDANSERPQDRAAAEA
                     LLTRVRPYVRYFSSSQYLNDLASGDVCVSIGWSGAIQQARARGAEAAQPVQVVYVIPK
                     EGAPLWSDLVQIPVDAPHPELAHVFIDYLMEPKVMAAITNTVGEANGNTASTPYVDEA
                     IRNNPAIYPTDETYQRLTVDRSWSPAQMREVDRAWTRIQTAK"
     gene            1497..1739
                     /locus_tag="PL152_10225"
     CDS             1497..1739
                     /locus_tag="PL152_10225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49702.1"
                     /translation="MRTTLDIEDDVLAAAKEIARRQGRTAGDVISSLARRALTGPGPE
                     TSVRETRATYGFKPFAADGKIVTNETIDDLRDREGV"
     gene            1739..2170
                     /locus_tag="PL152_10230"
     CDS             1739..2170
                     /locus_tag="PL152_10230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HQR49703.1"
                     /translation="MRGLLDVNVLIALLDEAHVHHRSATDWLTGNIRHGWASCPLTQI
                     GCIRIMSQPAYPNPQPAALVAERLRMAAQDRHHEFWSAGVDLLSGRSIAWEGVLGSRF
                     VTDAYLLSLAVAHRGRFVTFDRRVPVAAVPGAGERHLVVIE"
     gene            complement(2212..2385)
                     /locus_tag="PL152_10235"
     CDS             complement(2212..2385)
                     /locus_tag="PL152_10235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49704.1"
                     /translation="MTIRSYIESAAETEKRDAEYMEWKKKTIAQMDKQSGDRAEFREI
                     LEKSLLHELKFRK"
     gene            complement(2416..2571)
                     /locus_tag="PL152_10240"
     CDS             complement(2416..2571)
                     /locus_tag="PL152_10240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49705.1"
                     /translation="MPGGCWEVTGVKLKDGGTLAYADYLAGEWRANVESAKSKGWIKG
                     YLLFQNS"
     gene            complement(2660..>3003)
                     /locus_tag="PL152_10245"
     CDS             complement(2660..>3003)
                     /locus_tag="PL152_10245"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQR49706.1"
                     /translation="CHCSMCRRAHGAGFVTWVGFPRGAVKIEKGDDLLVRFRSSAKAR
                     RSFCSRCGSTLLFESENWPDETHVTLANLDSAEGLEPQAHAYWSSRAPWSDWSGKELP
                     TVDPPDHGEDA"
BASE COUNT          527 a         1000 c          963 g          513 t
ORIGIN      
        1 tcgaggacga cgaaggccac gagatcgagc tgcgcatcgt cggcccggac gagattgatc
       61 ccgcgaggcg ctatgtgagc atggactcgc cgctggcgcg cgcgctgatg cgccggcagc
      121 gcgacgagga attcacggtc gaagtgccgg ggggccggaa gacttacgtc gtgacggaga
      181 tccgctacga ggcgggggat gacgggtctt agccgcgtgc ttcgtcgcac ccgaacgggt
      241 gacaacgacg gatcgcggtc gtgatgtaat caggccagcc aagaacatcc gccggggagg
      301 cgactcgtgc gatccattgc ccacgctggc ctggtcgcgg cgctgctcgc gtcactgctg
      361 tcgtcggtgg cttcgtgcgg cagcggccac gacaaagctg ccacggcggc tgaagagaag
      421 gtcctgcacg tctacaactg gaacgactac atcgcgccga cgacgatcgc ggatttcgag
      481 acgcggaccg ggatcaaggt cgtctacgac gtgtacgact cgttcgaggt cctcgaaacc
      541 aagctcctga cgggtcactc gggctacgat gtcgtggtga cctccggcgg gcccacccgc
      601 aagctcatcg acgcgggcgc catcaagacg ctcgatcgct cgaagctccg gaacctggcg
      661 aacctggacc cgcggatcgt gcagctccag acgtactacg atccgggcaa tgcacacacc
      721 gtgccgtatc tcctcgggac gaccggcatc ggctacaacc ccgaccaggt cgagaaggct
      781 ctgggcacgc ggacgatcga cagcttcgcc gcggtgttcg atccggccat tgcgtccaag
      841 ctcgcgaagt gcggaatcac ctggctcgat tcgccggccg acctgttcca ggtggcgttt
      901 atctacctcg ggctcgacgc caacagcgag cgtccgcagg accgggcggc agccgaggcg
      961 ctgctcactc gagttcgccc gtacgtgcgt tacttcagtt catcgcagta tttgaacgac
     1021 ctcgccagcg gcgacgtgtg tgtctcgatc gggtggagcg gcgcaatcca gcaggcacgc
     1081 gcgcgcggcg cggaagctgc gcaaccggtc caagtggtct acgtcattcc aaaggagggc
     1141 gcgcccctct ggagcgacct ggtgcagatt cccgtcgatg cgccgcatcc cgaactggcc
     1201 cacgtgttca tcgattactt gatggagccc aaggtgatgg cggcgatcac gaacacggtc
     1261 ggcgaggcga acggcaacac cgcgtccacg ccgtacgtcg acgaggctat ccgcaataac
     1321 cccgcgatct atccgaccga cgagacgtac cagcggctga ccgtcgaccg ctcctggtcg
     1381 ccggcgcaga tgcgcgaggt ggaccgggcc tggacacgga tccagaccgc gaagtgagtt
     1441 ggcaaccagc cacgttgcgg gttcgcatct gatgccttat catcaccgca tcacatatgc
     1501 gcaccaccct cgacatcgaa gacgacgtcc tcgccgccgc gaaagaaatc gcgagacggc
     1561 agggacgcac ggccggcgac gtaatctcct cgctcgcgcg tcgcgcattg acggggccgg
     1621 gccctgaaac gagcgtgcgg gaaacgcgcg cgacctacgg attcaaaccg ttcgcggccg
     1681 acggcaagat cgtcacgaat gagaccatcg acgacttgcg cgaccgcgaa ggcgtctgat
     1741 gcgcggattg ctcgacgtca acgtgctcat cgcactgctc gatgaggcgc acgtccacca
     1801 tcggtccgca acggactggc tgaccgggaa catccgccac ggctgggcgt cgtgcccgct
     1861 cacgcagatc ggctgcatca ggatcatgtc ccaaccggcc tatcccaacc cgcagcccgc
     1921 cgcactcgtg gctgagcggc tccgcatggc cgctcaagac cgtcatcacg agttctggag
     1981 cgcgggcgtc gacctgctgt ccgggcgatc gatcgcttgg gagggcgtgc tcggatcgcg
     2041 gttcgtcacc gatgcgtacc tgctctcgct cgccgtcgcg catcgcgggc gattcgtcac
     2101 gttcgacagg cgcgtgccgg tcgcggcggt cccgggtgca ggggaacgcc acctcgtcgt
     2161 gatcgagtag gcacggcatg gttcgtctgg gcctccgccg gcccgccctt gtcactttcg
     2221 aaacttcagt tcgtgcagca gcgacttctc gaggatctcg cgaaactccg cgcgatcacc
     2281 tgattgcttg tccatctgcg cgatggtctt cttcttccat tccatgtact cggcgtcgcg
     2341 cttctctgtc tccgccgcgg actcgatgta gctgcggatc gtcaccagat acagatcggg
     2401 ctccccggct cgtggctacg agttctggaa cagaaggtat cccttgatcc agcccttcga
     2461 tttcgcagat tccacgttgg cgcgccattc gccggcaagg taatcggcat acgcgagcgt
     2521 gccgccgtcc ttgagcttca cgccggtcac ttcccagcag ccgccgggca ccaccgggta
     2581 ttggtccgtg acggcagtca ggtgggcccg agcgcaaggt ggacgacgct gcgacggcga
     2641 cctcggcccc agcgccatgt caggcgtctt ccccgtggtc cggcggatcg acggtcggca
     2701 gttccttgcc gctccagtcc gaccacggcg cgcggctcga ccagtaggcg tgtgcctgcg
     2761 gctcgagtcc ctcggcagag tcgaggttgg cgagcgtcac gtgcgtttcg tccggccaat
     2821 tctcggattc gaagagcagc gtcgacccgc agcggctgca gaaggagcgc ctagccttgg
     2881 cggacgatcg gaagcggacg agcaggtcgt cgcccttttc gattttcacc gcaccgcgtg
     2941 gaaatcccac ccaggtcacg aaccccgcgc cgtgcgcgcg gcggcacatc gagcagtgac
     3001 agt
//
LOCUS       DAOMFD010000234         3002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_16907_length_3002_cov_37.124533, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000234 DAOMFD010000000
VERSION     DAOMFD010000234.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16907_length_3002_cov_37.124533"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..463
                     /locus_tag="PL152_10250"
     CDS             137..463
                     /locus_tag="PL152_10250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49707.1"
                     /translation="MTRVRAVKSFGRPLAMLALVALLVAQVAAGMHAVKHLRSGDSTG
                     LPGGTHVQFCLECVSFAPLTSAHGGGVTALAVAPAGIEAFVRLLDTGAVVSRPHLAFR
                     SRAPPR"
     gene            533..1939
                     /locus_tag="PL152_10255"
     CDS             533..1939
                     /locus_tag="PL152_10255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49708.1"
                     /translation="MGKVPFHLAVAAYCAAMLPAVASAATDADVESLRKEIDALKAEY
                     AQRIAALESQVQQLQANPDVGATAAVDQQVAAAPAAPPAAANAATAFNPAISLVLTGN
                     YADLSRNPDDYAIQGFIPAGDGVGPGNRSFNLGESELTLAASIDPYFMGNLTASITSD
                     NSIELEEAWFRTTALPFGLNVKGGQFLSSIGYLNEVHSHNWDFVDLPLVYQAFFDGQM
                     HVQGVQAKWVAPLDEVLLEFGTESGNGNYFPGTSLTRNGLNGYNAYVHLGGDLGDTVA
                     WRTGLSYVDLHADDRSFDEPNPAGGDSTSAFTGASTTWIADAELKWTPIGDPRNQYLK
                     LQGEYMQRREDGHLSYAYDATDLDGSYRSDQSGWYLQAVYRFLPRWRIGARYDSMDSG
                     TMKIGLVQQGLLSPADFPLLLSGSPTRTSVMVDWSPTEFSRLRAQYAWDDASAGPTDD
                     QFFLQYIYALGAHGAHKF"
     gene            1949..2854
                     /locus_tag="PL152_10260"
     CDS             1949..2854
                     /locus_tag="PL152_10260"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HQR49709.1"
                     /translation="MIRKFIGASLAMALLVTQPAQAALKVLATTADWGSLATELGGDK
                     VNVYTATSALEDVHFVNAKPSLVARARTADLVVANGADLEIGWLPVLLQESGNTKIQP
                     GTPGYFEATAFVRLLDVPTAVDRSQGDIHPLGNPHIQLDPRNVAAVAKGLTARLAAID
                     PANAAYYQARGSDFQGRWQQAIARWTTEGAKLKGVPVVVIHTDQRYLLNWLGMVQLAT
                     IEPKPGVPPSAGYLAELVTRLSASKPTMILRNAYNDPKAVDWLSQKIGTPVVLLPFSV
                     GGTPEAKDLFGLFDDTLNRLLAAGK"
BASE COUNT          479 a         1082 c          970 g          471 t
ORIGIN      
        1 cagctgcgat aaccgctgct cgggcgatgc cgcccgtacg aggcgatggc ggcgacacgc
       61 cagaccgcgc gacgagcccc gacccactcg tcgaggatgg gcgaacacgc tgcgacttcg
      121 gctaacatcg cgcgccatga cgagggtgcg cgccgtcaag tcgttcggac gaccgctggc
      181 catgctggcc ctggtggcgc tgctcgtcgc gcaggtcgcc gcgggcatgc acgcggtcaa
      241 gcacctgcga agcggcgact ccaccggact cccgggtggt acccacgtcc agttctgcct
      301 cgagtgcgtg tcgttcgcgc cactcacctc ggcgcatggt ggcggtgtca ccgcgctcgc
      361 ggtcgcgccg gccggaatcg aggcattcgt gcgcctgctc gacacgggcg ccgtcgtttc
      421 ccgcccccat ctcgccttcc gctcccgcgc gcctcctcgc tgatcgatcg ccactgcccg
      481 tgcaccggcc cgacccgggc cggccgatcg tgttcatcga ggagattccc ccatgggaaa
      541 ggtcccattt catttggccg tcgcggcgta ttgcgcggcc atgctgccgg ccgtcgcgag
      601 tgccgcgacc gatgcggacg tcgagtcgct ccgcaaggag atcgacgcac tcaaggccga
      661 gtatgcgcag cgcatcgcgg cgctcgaatc ccaggtgcag caactgcagg cgaatcccga
      721 tgtcggcgcc accgccgctg tcgaccagca ggtggcggcg gcgcccgcgg ctccgccggc
      781 cgccgcgaac gctgcgacgg ccttcaatcc cgcgatctcg ctggtcctga ccggcaacta
      841 cgccgacctg tcgcggaacc cggacgacta cgcgatccag ggattcatcc cggcgggtga
      901 cggggtcggg cccggcaatc gcagcttcaa cctcggtgag tcggagctca cgctcgcggc
      961 cagcatcgac ccgtacttca tgggcaacct caccgcctcg atcacttcgg acaactcgat
     1021 cgagctcgag gaggcctggt tccgcacgac ggcgctgccg ttcgggctca acgtcaaggg
     1081 cggccagttc ctctccagca tcggctacct gaacgaggtc cactcgcaca actgggactt
     1141 cgtcgacctg ccgctcgtct accaggcatt cttcgacggc cagatgcacg tgcagggcgt
     1201 gcaggcgaag tgggtggcgc cgctcgacga ggtgctgctc gagttcggca cggagtccgg
     1261 caatggcaac tacttcccgg gcacgtcgct gacccgcaac gggctgaacg gctacaacgc
     1321 ctacgtgcac ctcggcggag acctgggcga cacggtcgcg tggcgcacgg gcctctcgta
     1381 cgtcgacctc catgccgacg atcgcagttt cgacgagccc aacccggccg gcggtgactc
     1441 gacgagcgcc ttcacgggcg catcgaccac ctggattgcc gatgccgaac tcaagtggac
     1501 gccgatcggc gatccgcgca accagtacct caagctgcag ggcgagtaca tgcagcgtcg
     1561 cgaggatggc cacctctcct acgcctacga cgcgaccgac ctcgacgggt cctaccgcag
     1621 cgatcagtcc ggctggtacc tgcaggcggt ctaccgcttc ctgccacggt ggaggatcgg
     1681 tgcccgctat gactcgatgg actccggcac gatgaagatc gggctggtgc agcagggcct
     1741 gctgagcccc gcggacttcc cgctgctgct gtccggctcg ccgacccgca cgtcggtgat
     1801 ggtggactgg agcccgacgg agttctcgcg gctgcgcgcg cagtacgcgt gggacgacgc
     1861 atcggccggg cccaccgacg accagttctt cctgcagtac atctacgcac tcggcgcgca
     1921 cggcgcccac aagttctagg agccaaccat gatccgcaaa ttcatcggcg ccagcctcgc
     1981 aatggcgctg ctggtcacgc aaccggcgca ggccgccttg aaagtgctcg ccaccaccgc
     2041 cgactggggc tcgctcgcca ccgagctcgg cggcgacaag gtgaatgtct acacggcaac
     2101 cagcgcgctg gaggacgtgc acttcgtgaa cgccaagccg agtctcgtcg cgcgtgcgcg
     2161 cacggctgac ctcgtcgtgg cgaacggcgc cgacctcgag atcggctggc tgccggtgct
     2221 gctccaggaa tccggcaaca cgaagatcca gcctggcacc ccaggctact tcgaggccac
     2281 ggcattcgtc cgtctgctcg acgtgccgac ggccgtcgat cgctcccagg gcgacatcca
     2341 cccgctcggc aatccccaca tccagctcga cccgcggaac gtcgctgccg tggccaaggg
     2401 cttgacggcg agactcgctg cgatcgatcc ggcgaatgcg gcgtactacc aggcacgcgg
     2461 gtccgatttc caggggcgct ggcagcaggc gatcgcgcga tggaccacgg aaggcgcgaa
     2521 gctgaagggc gtgccggtgg tcgtgatcca caccgaccag cgttacctgc tcaactggct
     2581 cggcatggtc cagctggcca cgatcgagcc caagcccggc gtgccgccga gcgccggcta
     2641 tctcgccgaa ctggtcacgc gcctgtcggc gagcaagccc acgatgatcc tgcgcaacgc
     2701 ctacaacgat ccgaaggccg tcgactggct gtcgcagaag atcggcacgc ccgtcgtgct
     2761 gctgccgttc tcggtcggcg gcacgccgga ggcgaaggac ctgttcggcc tgttcgacga
     2821 caccctcaac cggctgctgg cggcgggcaa atgagcgact tcacctcgac cctgggcatc
     2881 ctctggccgg cgctcgtcgc cggcttgctc gtcgccatct cgcacgttcc gctcggtcag
     2941 caggtgctgc ggcggggcat cgtgttcatc gacctcgcga tcgcgcaggt cgcggcgctc
     3001 gg
//
LOCUS       DAOMFD010000235         2988 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_17055_length_2988_cov_35.294579, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000235 DAOMFD010000000
VERSION     DAOMFD010000235.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2988)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2988
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17055_length_2988_cov_35.294579"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..608
                     /locus_tag="PL152_10265"
     CDS             <1..608
                     /locus_tag="PL152_10265"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: protein motif:HMM:NF014951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HQR49710.1"
                     /translation="RPQGLRDMNEKVYTAWFPECCAPTLITRDMGDMHAFLREHGRAV
                     CKPLYGMGGRSIFVLEAGDKNANVVFETLTEYGSRYAIVQRYIPDIVATGDARVILVD
                     GEPVPYALARIPSPADNRGNLAAGAKGVGRPLDERDRWLAGRIGPALRDRGMLFVGLD
                     VIGGYVTEINVTSPTGARELDRQFGLDVTGLLMDAIAKRRA"
     gene            612..1496
                     /locus_tag="PL152_10270"
     CDS             612..1496
                     /locus_tag="PL152_10270"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR49711.1"
                     /translation="MDLRSRIDRPEDRLMTAIFVAALVHGLVILGVRFSAPPPREQPL
                     PTLEVLLVPDGPAEKDNPAAGYIAQRSQHGSGTGRDRKRTSLPESSASLIEHQGEPGG
                     DSLDEESSTATSGGIQLLATTSPQAGRRVPGTDTSRASTAIPLDSRPLAQIGVNAYAA
                     DESLHLRGDPSQDDRLLADTRESLIAGYLDGWKRRVERVGTLNFPDAARRQALSGNPV
                     LEVAIRADGDLVQVLVRRSSGHRELDNAALRIVKLAAPFDPFPRAMRERYPVLRFAYE
                     WQFLKGRLDDGAVYTDAP"
     gene            1548..2108
                     /locus_tag="PL152_10275"
     CDS             1548..2108
                     /locus_tag="PL152_10275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HQR49712.1"
                     /translation="MPASYLTNQFLIAMPGMDDPNFAQTVTLVCDHSERGALGIVINR
                     PLSMDLGDVFAQLGLDASQSRVTSQHVLQGGPVQTDRGFVLHSPGPGWESTLPVSDRL
                     HLTTSRDILDALAGGGGPDRAVIALGYAGWDAGQLEDEVARNAWLTAPADETLLFDVP
                     AEERWQAAGRLLGINLLHLSSQAGHA"
     gene            2117..2554
                     /gene="ruvX"
                     /locus_tag="PL152_10280"
     CDS             2117..2554
                     /gene="ruvX"
                     /locus_tag="PL152_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HQR49713.1"
                     /translation="MAGPAEVLLAFDYGARRIGVAVGQSLTGSASPAGTVAVAAAPDW
                     VALEQLIRRWSPTRLLVGLPYNMDGSETVLTGVCRAFARELARRSGLPVDFVDERLTS
                     VAAQADLREARRSGARTRRVRREDIDANAARLILETWLREHRT"
     gene            2568..>2988
                     /gene="pyrB"
                     /locus_tag="PL152_10285"
     CDS             2568..>2988
                     /gene="pyrB"
                     /locus_tag="PL152_10285"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF014749.2"
                     /note="catalyzes the transfer of the carbamoyl moiety from
                     carbamoyl phosphate to L- aspartate in pyrimidine
                     biosynthesis; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HQR49714.1"
                     /translation="MTTDTGAELQRDAAGRLRHLLTLDGLSRDELEQLLDLAQFYVRT
                     PGDMPPRDQSLAGRTVANLFFEPSTRTRVSFELAARRLGADVVNLDLHSSSRVKGETV
                     LDTIYTLQSMLVDVFVMRDAEPGLPAYVAAHVAPHVSV"
BASE COUNT          443 a         1111 c         1018 g          416 t
ORIGIN      
        1 accggccgca gggcctgcgc gacatgaacg agaaggtcta cacggcctgg ttccccgaat
       61 gctgcgcgcc aacgctcatc acgcgcgaca tgggggacat gcacgcgttc ctcagggagc
      121 acggccgcgc ggtgtgcaag ccgctctacg ggatgggcgg gcggtcgata ttcgtcctgg
      181 aagctggcga caagaatgcc aacgtggtgt tcgagaccct caccgagtac ggcagccgtt
      241 acgcgatcgt gcagcgctac atccccgaca tcgtcgcgac cggcgatgcc cgcgtgatcc
      301 tcgtggacgg cgagccggtc ccctacgcgc tggcccgcat cccctcgccc gccgacaacc
      361 gcggcaacct cgccgccggc gccaagggcg tcggccggcc gctcgacgag cgcgaccgct
      421 ggctcgccgg ccgcatcggg cccgcactgc gcgaccgggg catgctgttc gtcgggctcg
      481 acgtcatcgg cggctacgtc acggagatca acgtgacgag tccgaccggc gcgcgcgaac
      541 tcgatcgcca gttcgggctc gacgtgacgg gcctgctcat ggacgcgatc gcgaagcggc
      601 gcgcctgacc gatggacctc cgctcacgca tcgaccggcc cgaagaccgc ctcatgacgg
      661 cgatcttcgt cgccgccctc gtgcacgggc tcgtgatcct cggcgtgcgc ttcagcgcgc
      721 cgccgccgcg cgagcagccg ctgccgaccc tcgaggtgct gctggtgccc gatggccccg
      781 cggagaagga caatcctgcc gctggctaca tcgcgcagcg cagccagcac ggctccggca
      841 cgggtcgcga ccgcaagcgc acctcgctgc cggaatcctc ggccagcctc atcgagcacc
      901 agggagagcc cggcggcgac tcgctggacg aggaaagctc gacggcgacg tccggaggca
      961 tccagttgct ggccaccacc tcgccgcagg cggggcgcag ggtgcctggc accgatacct
     1021 cccgggcttc gaccgcaatc ccgctcgact cgcgtcccct ggcccagatc ggcgtcaacg
     1081 cctacgcggc cgacgaatcc ctgcacctca ggggcgaccc gtcccaggac gaccggttgc
     1141 ttgccgacac gcgcgagtcg ctgatcgccg gttacctgga cggctggaag cggcgggtcg
     1201 agcgcgtcgg cacgctcaac ttcccggacg cggcgcggcg ccaggccctg tcgggcaacc
     1261 ccgtgctcga ggtggccatc cgcgcggacg gcgacctggt gcaggtactg gtgcgccgca
     1321 gcagcggcca ccgcgaactc gacaacgccg cgctgcgcat cgtcaagctg gccgcgccgt
     1381 tcgacccctt cccgcgggcc atgcgcgagc gctaccccgt gctgcgcttc gcctacgaat
     1441 ggcagttcct gaagggccgc ctcgacgatg gagccgtgta caccgacgcc ccctgacgtc
     1501 acgcccggcg cttgtcccgc gccgccggcc ggcaggatac ttcgcacatg ccggcgagct
     1561 acctgacgaa ccagttcctg atcgcgatgc cgggcatgga cgatccgaac ttcgcccaga
     1621 cggtgacgct cgtctgcgat cacagcgagc gtggcgcgct cggcatcgtg atcaaccgcc
     1681 ccctcagcat ggacctggga gacgtgttcg ctcagctcgg actcgacgct tcgcaatcgc
     1741 gagtgacgag ccagcacgtg ctgcagggag gcccggtgca gaccgaccgc ggattcgtgc
     1801 tgcacagccc ggggcctgga tgggagtcga cgctgccggt ctccgatcgg ctgcacctca
     1861 cgacctcgcg cgacatcctc gatgcgctgg ccggcggggg cggcccggac cgagccgtga
     1921 tcgcgctcgg gtacgcgggc tgggacgccg ggcagctcga ggacgaagtc gcgcgcaatg
     1981 catggctcac cgcgcctgcg gacgagacct tgctgttcga cgtcccggcc gaggagcgct
     2041 ggcaggcggc cggtcgcctc ctcggcatca acctgctgca cctgagcagc caggccgggc
     2101 acgcctgacc cggcccgtgg caggcccagc ggaagtcctc ctcgccttcg actacggagc
     2161 ccgccgcatc ggcgtggcgg tcgggcagtc gctgaccggt tcggccagcc ccgccgggac
     2221 ggtggcggtt gccgcggctc cggactgggt tgcactggag cagttgatcc gccgctggtc
     2281 gccgacgcgg ctgctggtgg ggctccctta taatatggac ggttccgaga ccgtcctcac
     2341 cggagtctgc cgcgccttcg cacgcgaact cgcgcggcgc tccggcctgc cggtggactt
     2401 cgtggacgag cgactgacct cggtcgcggc gcaggccgac ctgcgcgagg cgaggcggtc
     2461 gggcgcgcga acccggcggg tgaggcgcga ggacatcgac gcgaacgccg cacgcctgat
     2521 cctcgagacc tggctgcgcg aacaccgtac ctgagcagga gcacgacttg accaccgaca
     2581 ccggagccga actgcagcgc gacgccgcgg gccggctgcg ccacctgctc accctcgacg
     2641 gcctgtcccg cgacgaactc gagcagctgc tcgacctcgc gcagttctac gtgcgcacgc
     2701 ccggcgacat gccgccgcgc gaccagagcc tcgcgggacg cacggtcgcc aacctgttct
     2761 tcgagccgag cacgcgcacc cgagtctcct tcgagctggc cgcgcggcgc ctcggcgccg
     2821 acgtcgtgaa cctcgacctg cactcgtcct cccgcgtgaa aggtgagacg gtgctcgaca
     2881 cgatctacac gctgcagtcg atgctggtgg acgtgttcgt gatgcgcgac gcggagcccg
     2941 ggctgcccgc ctacgtggcc gcgcacgtcg cgccccacgt gagcgtgc
//
LOCUS       DAOMFD010000236         2986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_17073_length_2986_cov_26.955647, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000236 DAOMFD010000000
VERSION     DAOMFD010000236.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2986
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17073_length_2986_cov_26.955647"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1093)
                     /locus_tag="PL152_10290"
     CDS             complement(<1..1093)
                     /locus_tag="PL152_10290"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HQR49715.1"
                     /translation="MNTIGEVSLDAALNAGEHTLPRLLQHWAQTTPDALALREKDLGV
                     WSRVTWRQYHDTARRFALGLLHLGFRRGERLAIASEDTPEWMYADLATQAIGGVTVGI
                     YPTNPWPELQYIVHHSNSRFVVCGDQEQVDKVLDAMGKHEVGLPEVAKIIAVDMKGMR
                     NYPRDKLLSFDEVLEFGDRHAAASPEQVRAFDDGIAATQPDDTCMLVYTSGTTGPPKG
                     AMLSHRNLLVTTDKLRRHFGLDGTNMQVVCYLPLCHVAERAFSTVMHMMTGGVVNYAE
                     SIDTVQINLREIAPTVFLGVPRIWEKLQQGIVMRMQDATRFQRWAFERCFASGKALFE
                     RVEQRGGERSWADRLQFFVLWLLMFRNLQK"
     gene            complement(1090..1872)
                     /locus_tag="PL152_10295"
     CDS             complement(1090..1872)
                     /locus_tag="PL152_10295"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR49716.1"
                     /translation="MHHDDALLKVENLSKRFGGVEAVAGVGFHVRRGEIYSLIGPNGA
                     GKTTTFNMISGVVPPSGGTVHFDGRPITGTPAHRMAALGIGRTFQNLAVFKHATVVQN
                     LLVGRHCHMRSNVFDAAWFFGRARREELEHRRKVEEIIDFLEIEDIRDMPVGALSYGM
                     QKRVELGRALATEPKLLLLDEMVSGMNTEETEDIARFVLDIRDELGIAVLMVEHDMGI
                     VMDISDRICVLNFGRKIAEGTPGEVSADPAVIDAYLGGAKAA"
     gene            complement(1881..2672)
                     /locus_tag="PL152_10300"
     CDS             complement(1881..2672)
                     /locus_tag="PL152_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012203085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HQR49717.1"
                     /translation="MLKLTNVQIVYDNTIEAVREASLALTGARIVALLGANGAGKSTT
                     LKAISGVLYPEDGEIKGGDISFEGRTLRGVPPDRIVRAGIVMVPEGRRLFDQLTVEEN
                     LLMGGYTRSRADSRRGLERVYEMFPRLTAKRTTVSGYLSGGEQQMVAIGRALMSSPKL
                     LMLDEPTLGLAPQICEEIFGIVTRLCRQDGVSILLVEQNAQMALTIADDGYIMENGRI
                     VMDGPAEKLLRNEDVREFYLGFGEGGSRKNMRDVKHYKRRKRWLS"
BASE COUNT          461 a          989 c         1017 g          519 t
ORIGIN      
        1 acttctgcag gttgcggaac atcagcagcc acagcacgaa gaactgcagc cggtcggccc
       61 agctgcgctc gccgccgcgc tgctcgacgc gctcgaacag ggccttgccc gaggcgaagc
      121 agcgctcgaa agcccagcgc tggaagcggg tggcgtcctg catgcgcatc acgatgccct
      181 gctgcagctt ctcccagatg cggggcacgc ccagaaacac ggtcggcgca atctcgcgca
      241 ggttgatctg caccgtgtcg atcgactcgg cgtagttgac gacgccgccg gtcatcatgt
      301 gcatcacggt ggagaaggcg cgctcggcca cgtggcacag cggcaggtag cagaccacct
      361 gcatgttcgt gccgtcgagc ccgaagtggc ggcgcagctt gtcggtggtg acgagcaggt
      421 tgcggtgcga cagcatcgcg cccttgggcg gcccggtggt gccagaggtg tacaccagca
      481 tgcaggtgtc gtcgggttgc gtcgccgcga tgccgtcgtc gaaggcgcgc acctgctcgg
      541 gcgaggcggc ggcgtggcgg tcgccgaact cgagcacctc gtcgaacgag agcagcttgt
      601 cgcgcgggta gttgcgcatg cccttcatgt cgaccgcgat gatcttggcg acctcgggca
      661 gcccgacctc gtgcttgccc atcgcgtcaa gcaccttgtc gacctgttcc tggtcaccgc
      721 acacgacgaa gcggctgttc gagtggtgga cgatgtactg cagctccggc caggggttgg
      781 tcgggtagat gcccaccgtg accccgccga tcgcctgcgt ggccaggtcg gcatacatcc
      841 actcgggcgt gtcctcgctg gcgatggcca gccgctcgcc gcgccggaag cccaggtgca
      901 gcaggccgag cgcgaagcgc cgcgccgtgt cgtggtactg ccgccaggtc acgcggctcc
      961 agacgccgag atccttctcg cgcagcgcca gcgcatcggg cgtggtctgc gcccagtgct
     1021 gcagaagccg gggtagggtg tgttcgccgg cgttcagcgc ggcgtcgagc gagacctctc
     1081 cgatggtgtt catgcggctt tcgccccgcc caaataggca tcgatcacgg ccgggtcggc
     1141 cgatacctcg cccggcgtgc cctcagcgat cttgcggccg aagttgagca cgcagatgcg
     1201 gtcggagatg tccatcacga tgcccatgtc gtgctcgacc atcagcacgg cgatgccgag
     1261 ctcgtcgcgg atgtcgagca cgaagcgcgc gatgtcctcg gtctcctcgg tgttcatgcc
     1321 cgagaccatc tcgtcgagca gcagcagctt gggctcggtg gccagcgccc ggccgagctc
     1381 gacgcgcttc tgcatcccgt aggacagcgc gcccaccggc atgtcgcgga tgtcctcgat
     1441 ctcgaggaag tcgatgatct cctcgacctt gcgccggtgt tccagttcct cgcgccgcgc
     1501 gcggccgaag aaccaggcgg catcgaacac gttgctgcgc atgtggcagt ggcgcccgac
     1561 aagcaggttc tgcaccaccg tggcgtgctt gaacacggcc aggttctgga acgtgcggcc
     1621 gatgcccagt gccgccatgc ggtgggcggg cgtgccggtg atcgggcggc cgtcgaagtg
     1681 caccgtgccg ccgctcggcg gcacgacccc gctgatcatg ttgaaggtgg tggtcttgcc
     1741 cgcgccgttg ggcccgatca acgagtagat ctcgccgcgc cgcacatgga agccgacgcc
     1801 ggcgacggcc tcgacgccgc cgaagcgctt ggacaaattc tcgaccttca gcagggcgtc
     1861 gtcgtggtgc atcgcgccgc tcacgacagc cagcgcttgc ggcgcttgta gtgcttgacg
     1921 tcacgcatgt tcttgcgcga gccgccttcg ccgaagccga ggtagaactc gcgcacgtcc
     1981 tcgttgcgca gcagcttttc cgcggggccg tccatcacga tgcggccgtt ctccatgatg
     2041 tagccgtcgt cggcgatggt cagtgccatc tgcgcgttct gttcgacgag caggatgctg
     2101 accccatcct ggcggcacag gcgagtgacg atgccgaaga tctcctcgca gatctgcggc
     2161 gccaggccca gcgtgggctc gtcgagcatc agcagcttcg ggctcgacat cagcgcgcgg
     2221 ccgatggcca ccatctgctg ctcgccgccc gagagatagc ccgataccgt cgtgcgcttg
     2281 gccgtcaggc gcgggaacat ctcgtagacc cgctcgagac cgcgccgcga atcggcccgg
     2341 ctgcgcgtat agccgcccat caacaggttt tcctcgacgg tgagctggtc gaacaggcgc
     2401 cgaccttcgg gcaccatgac gatgccggcg cggacgatgc ggtcgggggg gacgccccgc
     2461 agcgtgcggc cttcgaagct gatgtcgccg cccttgatct cgccgtcttc ggggtagagc
     2521 acgccgctga tcgccttcag ggtggtcgac ttcccggccc cgttggcgcc gagcagggcg
     2581 acgatgcgcg ccccagtcaa cgccagcgag gcttcgcgca cggcttcgat cgtgttgtcg
     2641 tagacgatct ggacgttggt cagcttgagc atttcctcga cgcggacgac ggtgccgggg
     2701 cagggccggc gaatggcgtc aatctaacgg ctgttagatc aaaaacaatc gagtgcttac
     2761 cctagcgttc gccggcggga gcaataggca ggccgccggc ggaccccgag ggtgccgaac
     2821 ctcgctgggg gaactcttca gccagtgaac ttctggcgga agtcgggctt gcgcttctcg
     2881 aggaaggcgc gcacgccttc cttcgactcg gcagtttcgt agtagaggct gagcgcctgc
     2941 atgcccaggc tgccgatgcc gcggatgtgc tcggtcgagg cgttga
//
LOCUS       DAOMFD010000237         2942 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_17495_length_2942_cov_37.649809, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000237 DAOMFD010000000
VERSION     DAOMFD010000237.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2942)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2942
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17495_length_2942_cov_37.649809"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(8..577)
                     /gene="efp"
                     /locus_tag="PL152_10305"
     CDS             complement(8..577)
                     /gene="efp"
                     /locus_tag="PL152_10305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016655583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HQR49718.1"
                     /translation="MSSYNTNEFRSGLKIILDGDPYTIVENEFVKPGKGQAFNRVRIR
                     NLKTGRTLERNFKSGETVEAADVVETDMQYLYNDGDFWHFMVPDTFEQHTAGKEALGE
                     ASIWLKDGTMCKVMLWNGVPLAVDPPAHVELKIVETDPGLRGDTATGGQKPAKLETGA
                     VVRVPLFLNEGEVIRVDTRSGAYLSRAKG"
     gene            872..2929
                     /locus_tag="PL152_10310"
     CDS             872..2929
                     /locus_tag="PL152_10310"
                     /inference="COORDINATES: protein motif:HMM:NF012772.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HQR49719.1"
                     /translation="MADNGGIPLLVLSRQQDPVEIINSTLRNAGHPVHCAWARDLTAL
                     GEALAEAKPQLMFLCVGDEDEARTALDVRSRFATHVPAILVRESVGESDLARAIEMGA
                     QDVVTLAARPRLEAVAVRELRSARLDGALAGTLASARQYRDQMKAFMTGSADAIAHVQ
                     EGIVVDVNPAWVELFGHEDAGAILGQPLMDFFHQRSHAALKGALVAAAHGRWTDHSLA
                     SVAVLPGGSELAVELELERFEFDGEPAVRLRVATQKRDLKSLTSQLEEALRFDTATGL
                     LKRQAFREQATTQSAQVLKAGLRAVVYFEPDKLASLDADIGPLAIEDLMDELGSQLRA
                     LLQPGDIAGRVTRRGFAVLVERGNTRDLDAWVARVLQRMAEHVFQPGDHHSATITCSA
                     GATVLNSQGEVLAGPLESAVKACRAAAAGGGNRLLRPDASGRTPDVVEADQGWATQIK
                     AALMANRFRLVQQPIASLVGEGDAMFDLVVRMLDDKGNEVLPSEFLAAAERTDLMKNI
                     DRWVIGAAMSFCAARKPSRVFVRVSRDSLRDQTLGTWLHQQIKASGVEPSRVVIEIGE
                     ELATKHLKEARELRALVTHLGFGFAIENFGTGRDSSQLLKHLPSTFVKIDGSLMQGLA
                     NDRKLQEQVRGLVELATGRKAATIAERVEDANTMAVLWQLGVEFVQGYFVSSPEEVVL
                     EAR"
BASE COUNT          460 a         1008 c         1019 g          455 t
ORIGIN      
        1 cgccggatca gcccttggcg cgggacaggt acgcgccgga gcgcgtatcg acgcggatga
       61 cctctccttc attcaggaag agcggtacgc gcacgaccgc gccggtctcg agcttcgccg
      121 gtttctgccc gccggtggcg gtatcgccgc gcaggcccgg atccgtctcg acgatcttga
      181 gttccacgtg cgcgggcgga tccacggcaa gcggtacgcc gttccacagc atgaccttgc
      241 acatggtgcc gtccttgagc cagatcgacg cctcgccgag cgcctccttg ccggccgtgt
      301 gctgctcgaa ggtgtccggc accatgaagt gccagaagtc cccgtcgttg tagaggtact
      361 gcatgtcggt ctcgacgacg tccgccgcct cgaccgtctc gcccgacttg aagttgcgct
      421 cgagcgtccg ccccgtcttg aggttgcgga tgcggacacg gttgaaggcc tggcccttgc
      481 ccggcttcac gaactcgttc tcgacgatgg tgtacgggtc gccgtcgagg atgatcttga
      541 gacccgagcg gaattcgttc gtgttgtagc tggacatggt gctggaaccg ggggttggtg
      601 gggccgaact gcccggcccg gccctcgatc ggacccggct gtggcaagcc gggcattgta
      661 cccgggcccg gtggatgccg tcagccccgg ggacccgtcg aggtcggagg cagcggcccc
      721 cagacaggcg tcaggaggca cgtctggccc cggcggtcgg ggtcagggtg ccacctgcgt
      781 cacgttagcc gccaaaaacc cggtgctata gtcccgccgc attgcgcagc agcaaccggg
      841 ccggcccgcc ggccccgaag agggttccag attggccgac aacggtggca tccccctcct
      901 ggtcctgtcc cgccagcagg acccggtcga gatcatcaac agcacactcc gcaacgccgg
      961 ccacccggtg cactgcgcgt gggcccgcga cctgacggcc ctcggtgagg ccctggccga
     1021 ggccaagccg caactcatgt tcctgtgtgt cggggacgag gacgaggcca ggacggcact
     1081 cgacgtccgc agccgatttg ccacccacgt gccggccatc ctggtccgcg agtcggtcgg
     1141 ggagtccgac ctggcgcgag ccatcgagat gggcgcccag gacgtcgtga ccctggccgc
     1201 gaggccgcgt ctcgaggccg tcgcggtccg cgagctgcgc tccgcccgcc tcgacggtgc
     1261 gctcgcgggc acgctggctt cggcacgcca gtaccgcgac cagatgaagg cgttcatgac
     1321 cggctcggcg gacgccatcg cccacgtcca ggaaggcatc gtggtcgacg tcaatccggc
     1381 gtgggtcgag ctcttcggcc atgaagatgc gggggccata ctcggtcagc cgctcatgga
     1441 cttcttccac cagcgcagcc acgcggcgct caagggagcc ctcgtcgcgg cggcgcatgg
     1501 acgctggacg gaccactcgc tcgcgagcgt tgccgtgctg cccggcggct cggagctcgc
     1561 cgtggaactc gagctcgagc gcttcgagtt cgacggcgaa ccggcggtgc gcctgcgcgt
     1621 cgcgacgcag aagcgcgacc tgaaatcgct caccagccag ctcgaggagg ccctgcgctt
     1681 cgatacggcg actgggctgc tgaagcgaca ggcatttcgc gaacaggcca cgacccagtc
     1741 cgcgcaggtg ctgaaggccg gactgcgcgc cgtcgtctac ttcgagccgg acaagctggc
     1801 gtcgctcgac gcggacatcg gtccccttgc gatcgaggac ctcatggacg aactcggctc
     1861 gcagctgcgc gcgctgctgc agcccggaga catcgcagga cgcgtcacca ggcggggatt
     1921 cgcggtgctg gtcgagcgcg gcaacacgcg cgacctcgac gcgtgggtcg ccagggtcct
     1981 gcagcgcatg gccgaacacg tcttccagcc gggcgaccat cactcggcga cgatcacctg
     2041 cagcgccggt gccacggtgc tgaattccca gggcgaagtg ctcgcgggcc cgctcgagtc
     2101 ggcggtcaag gcctgtcgcg ccgccgcggc cggaggcggc aatcggttgc tgcgcccgga
     2161 cgcaagcggt cgcacgccgg acgtcgtgga ggccgaccag ggctgggcga ctcagatcaa
     2221 ggccgcgctc atggccaacc ggttccggct ggtgcagcag ccgatcgcga gcctggtcgg
     2281 cgaaggcgac gcgatgttcg acctcgtcgt gcgcatgctc gacgacaagg gcaacgaggt
     2341 gctgccgtcg gagttcctcg ccgccgccga gcgcaccgac ctcatgaaga acatcgaccg
     2401 ctgggtgatc ggcgcggcga tgtcgttctg cgccgcgcgc aagccgagcc gggtcttcgt
     2461 gcgcgtctcg cgcgactcgc tgcgcgacca gacgctgggc acctggctgc accagcagat
     2521 caaggcctcg ggcgtcgagc cctcgcgcgt ggtgatcgaa atcggcgaag aactcgcgac
     2581 caagcacctc aaggaggcgc gcgagttgcg cgcgctcgtg acgcacctgg gcttcgggtt
     2641 cgcgatcgaa aatttcggca ccggccgcga ctcgtcgcag ctgttgaagc acctgccatc
     2701 gaccttcgtg aagatcgacg gctcgctcat gcagggcctc gccaatgacc gcaaactcca
     2761 ggagcaggtc cgcgggctgg tcgagctggc cactggccgg aaagccgcga ccatcgccga
     2821 gcgcgtcgag gatgccaaca cgatggccgt gctgtggcag ctcggcgtgg agttcgtgca
     2881 gggctatttc gtgtcgagcc ccgaggaagt cgtgctcgaa gccaggtgag ccgtgaccaa
     2941 cg
//
LOCUS       DAOMFD010000238         2929 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_17631_length_2929_cov_32.142658, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000238 DAOMFD010000000
VERSION     DAOMFD010000238.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17631_length_2929_cov_32.142658"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="PL152_10315"
     CDS             complement(<1..542)
                     /locus_tag="PL152_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HQR49720.1"
                     /translation="MFEPQPPLSSPELYINRELSFLEFDRRVLAQAEDPGVPLLERLR
                     FLAISCSNLDEFFEIRVAGLKQRRELGSLGGGPDGMPVDDQLAAIRAESLRLVEAQYQ
                     VFNRELQPQLRAHGIHILRASEWTPAQIDWLSAYFDREVEPVLTPLGLDPARPFPRIL
                     NKSQNFIVELSGSDAFGRDAA"
     gene            complement(642..1976)
                     /locus_tag="PL152_10320"
     CDS             complement(642..1976)
                     /locus_tag="PL152_10320"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HQR49721.1"
                     /translation="MRSIDLNDQRPAAPAADTQDYRRWLVAGMSFPLLAAAVAFALVR
                     APAQDAPPAIDLTPLVATSVAPPVATVEDEGPDSVEVTVQRDDTLDQIFRSVGIDLGT
                     LAELRSRPEVRKALDVLRPGDIITLTHVNGALQSLNRQISDTLTLQVSRAGDGYAVNY
                     IENPLETEVVGHRAVIDSSLFEAGQKAGISSQTIMTLANQIFGFDIDFALDIRQGDEF
                     SVLYEQKYQDGHYVSDGKVLAAEFVNQGKTHRAVWFQSKDGEVKGYFTPDGKGMRTAF
                     LRAPLDFTRVSSAFNSRRLHPVLGIIRAHKGTDYAAPTGTPIWAAGNGRVVFAGRKGG
                     YGNVVVIDHGHGVSTVYGHMSRFARSLRSGRQVSQGETIGYVGMTGLATGPHLHYEYK
                     VNGVQRNPAKLAMPRTEVPAQYMGEFRAGAEVALAKLDLTSGTGPQQRLAAR"
     gene            2169..>2929
                     /gene="tyrS"
                     /locus_tag="PL152_10325"
     CDS             2169..>2929
                     /gene="tyrS"
                     /locus_tag="PL152_10325"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018054613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HQR49722.1"
                     /translation="MSDSEPMLPAQEQLAELRRGTAEILLEPDLEKKLARGRPLRIKA
                     GFDPTAPDLHLGHTVLINKMALFQRLGHEVTFLIGDFTGLIGDPTGRNATRPPLTRED
                     IQANARTYEAQIFKILDPKLTKVDFNSRWMGELGAAGMIQLAAKHTVARMLERDDFAK
                     RYRSGQPIAIHEFLYPLVQGYDSVAMRTDVELGGTDQKFNLLVGRQLQEAYGQEPQVV
                     ITMPLLEGLDGVNKMSKSMGNYIGITEPPDDMFGKL"
BASE COUNT          459 a          976 c         1035 g          459 t
ORIGIN      
        1 gccgcgtcac ggccgaaggc gtcgctgccg gacagctcga cgatgaaatt ctggctcttg
       61 ttcaggatgc gcgggaacgg gcgcgccggg tcgaggccga gcggcgtgag cacgggctcg
      121 acctcccggt cgaagtaggc gctcagccag tcgatctggg cgggcgtcca ttccgatgcc
      181 cgcaggatgt ggatgccgtg cgcgcgcaac tggggctgca gctcgcgatt gaagacctgg
      241 tactgggctt cgaccaggcg cagggattcc gcgcggatcg ccgccagctg gtcgtccacg
      301 ggcatcccgt cggggccgcc gccgagcgat ccgagttccc gccgctgctt gaggcccgcc
      361 acgcggatct cgaagaactc gtcgaggttg ctgcaggaaa tggccaggaa gcgcagccgc
      421 tcgagcaggg gcacgcccgg gtcctcggcc tgcgcgagca cgcgacggtc gaactcgaga
      481 aacgacagtt cccggttgat gtagagctcg ggtgagctga gcggcggctg tggttcgaac
      541 atggtccggt gccggtggca gcgcccagtc tgccacggga tcgctgcgga tcgatgacgg
      601 cggacccgcg gctgggcggg ccgccccgcc gctggctggg atcagcgggc ggcgagcctc
      661 tgctgcggcc cggtgccgct cgtcaggtcc agtttggcga gcgcgacttc ggcgccggcc
      721 cggaactcgc ccatgtactg cgccggcacc tcggtgcgcg gcatcgcgag cttggcggga
      781 ttgcgctgca cgccgttcac cttgtactcg tagtgcaggt gcgggcccgt ggccaggccg
      841 gtcatgccga cgtagccgat ggtctcgccc tggctcacct ggcgcccgga gcgcagcgag
      901 cgggcgaagc gcgacatgtg gccgtacacg gtcgacacgc cgtggccgtg gtcgatcacc
      961 accacgttgc cgtagccgcc cttgcggccg gcgaacacca cgcggccgtt gccggcggcc
     1021 cagatcggcg tgcccgtggg tgcggcgtag tccgtgccct tgtgggcgcg gatgatgccg
     1081 aggaccgggt gcaggcggcg gctgttgaac gccgaactca cgcgcgtgaa gtcgagcggt
     1141 gcgcgcagga aggccgtgcg catgcccttg ccgtcgggcg tgaagtagcc cttgacctcg
     1201 ccgtccttcg actggaacca cacggcgcga tgcgtcttgc cctggttcac gaactcggcg
     1261 gcgagcacct tgccgtcgct cacgtagtgg ccgtcctggt acttctgctc gtagagcacg
     1321 ctgaactcgt cgccctggcg gatgtcgagc gcgaagtcga tgtcgaaccc gaagatctgg
     1381 ttggcgagcg tcatgatcgt ctgcgacgag atgccggcct tctgcccggc ctcgaacagc
     1441 gacgagtcga tgaccgcgcg atggccgacg acctcggtct cgagcggatt ctcgatgtaa
     1501 ttgaccgcgt agccgtcgcc ggcgcgcgag acctgcagcg tcagcgtgtc gctgatctgc
     1561 cggttgagcg actgcagcgc gccgttcacg tgggtcagcg tgatgatgtc gccgggacgc
     1621 agcacgtcga gtgccttgcg cacctcggga cgcgaacgca gctccgcgag ggtgccgagg
     1681 tcgatgccga cggaccggaa gatctggtcg agcgtgtcgt cgcgctgcac cgtgacttcg
     1741 acgctgtcgg gaccttcgtc ctcgaccgtc gccacgggcg gtgccacgct ggtcgccacg
     1801 agcggggtga ggtcaatcgc gggcggggcg tcctgcgcag gcgcgcgcac cagcgcgaat
     1861 gccaccgcag ccgcgagcag cgggaagctc atgccggcga ccagccagcg gcggtaatcc
     1921 tgggtatcgg cggccggggc tgccgggcgt tggtcgttga ggtcgatcga tcgcaagtgg
     1981 cagtccccgc gcaagagtgg gcgtaagtta gttgcgctgg cgcgacgagt caacgcctgg
     2041 ttcacagttt ttttcgagcc gggacagcca gttgcatcgc acttgacaag actgtgacag
     2101 ctgtctcggg cagggccgcg attgcctgcc cggggcgaac cccctacagt ccgccggccc
     2161 tcatcacctt gagcgactcc gagccgatgc tgcccgccca ggaacaactc gcggaactgc
     2221 gccgcggcac cgcggaaatc ctgctggagc ccgatctcga gaaaaagctc gcgcgcggcc
     2281 ggccgctgcg catcaaggcg ggcttcgacc ccaccgcgcc cgacctgcac ctcgggcaca
     2341 ccgtgctcat caacaagatg gcgctgttcc agcgcctggg ccacgaggtc acgttcctga
     2401 tcggcgactt caccgggctg atcggggacc cgaccggccg caacgcgacc cgcccgccgc
     2461 tcacgcgcga ggacatccag gccaacgcgc ggacctacga ggcgcagatc ttcaagatcc
     2521 tcgacccgaa gctgacgaag gtggacttca attcccgctg gatgggcgag ctcggcgcgg
     2581 cgggcatgat ccagctggcc gcgaagcaca ccgtcgcgcg catgctcgag cgcgacgact
     2641 tcgccaagcg ctaccggtcg gggcagccga tcgcgatcca cgagttcctc tatccgctgg
     2701 tgcagggcta cgactccgtc gcgatgcgca ccgacgtcga gctcggcggg accgaccaga
     2761 agttcaacct gctggtcggc cggcagctgc aggaggccta cgggcaggaa ccgcaggtgg
     2821 tgatcacgat gccgctgctc gaggggctcg acggcgtcaa caagatgtcg aagtccatgg
     2881 gcaactacat cggcatcacc gagccgccgg acgacatgtt cggcaagct
//
LOCUS       DAOMFD010000239         2918 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_17754_length_2918_cov_30.040866, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000239 DAOMFD010000000
VERSION     DAOMFD010000239.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2918)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17754_length_2918_cov_30.040866"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /gene="rfbA"
                     /locus_tag="PL152_10330"
     CDS             <1..867
                     /gene="rfbA"
                     /locus_tag="PL152_10330"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HQR49723.1"
                     /translation="RLYPITLAVSKQLLPVFDKPMIYYPLSLLMLAGIRDVLVIHVPN
                     EGERFRNLLGDGSHLGMTFTYAAQARPEGIAQAYVIGRDFVADQPSCLVLGDNILHGY
                     GLSDMLARAASRRHGATVFGYWVRDPQRYGVAEFDSSGRVIDIVEKPAKPASNFAVVG
                     LYFYDGRAPEFAASLRPSARGELEITDLNRCYLADGSLRIEQLGRGYAWLDAGTHESL
                     LQASGFVETLEMRQGLRICCPEEIAWRNGWISSDELAKLAGTMAGNSYGRYLLDLIEQ
                     DPGLWRGAKSPA"
     gene            complement(939..>2918)
                     /locus_tag="PL152_10335"
     CDS             complement(939..>2918)
                     /locus_tag="PL152_10335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HQR49724.1"
                     /translation="LLYHADFAGFRGGFVGVDIFFVISGFLITGLILPEVVQGTFSVR
                     SFYERRIRRIFPALYTVLAFTAVVAVLLLLPTDLKSFARSLVATTYFASNFFFNSQAG
                     YFDTDAHSKPLLHTWSLAVEEQYYILFPLCLVAVQRYLGGRHKLFVWLVVLGSLYLSV
                     TQTPTHPDRSFYLAHTRAWELGLGALLALGAFPRAHRRVTRNAAALVGAALVVFAVGW
                     FSAAIQFPGIAATVPCVGAALIIWSGTGGPNVVGRILQTKPFVAIGLISYSLYLWHWP
                     LLVFAEYWTIRDLTRPEAAGVLVIAAVAAALSWRYVERPFRGKSGVLTRRALFAVAAA
                     AMLVTAGLGRFVVSNAGFPGRLDPTTASLMAGIDDRRPRNWKCGNRNIRDVRSGDLCR
                     VGASGDVLPSFVIWGDSHARAMADVVGEAAARGGRAGLLATRNGCGPLLGLDREPDPQ
                     ARRCPEFAAAVLQFIADDPALTDVILIGRWAGYAEGTRYKNERGSGSSIYLRDAETRE
                     LSMTENQRVFARTLETTVDSLRAHGKTVWLVSAVPEVGWNVPSVLARMHRQDRAFSIA
                     PTLEEYRLRQQHVTPVLQRLQAAGKLRILSPDEVLCQAGHCAIVESGRPLYSDDDHLS
                     LTGAAKLSGMFTQIFPPAADAGASEAATTDVTGGH"
BASE COUNT          529 a          963 c          979 g          447 t
ORIGIN      
        1 cgcctgtacc cgatcacgct cgcggtcagc aagcagctgc tgccggtgtt cgacaagccg
       61 atgatctact acccgctgag cctgctgatg ctcgcgggaa tccgggacgt gctggtcatt
      121 cacgtcccga acgagggcga acggtttcgc aacctgctcg gcgacggcag tcacctgggc
      181 atgacattca cgtacgccgc acaggcgcgt ccggaaggga tcgcgcaggc gtacgtgatc
      241 ggcagggatt tcgtcgcgga ccagccgagt tgcctcgtcc tgggcgacaa catcctccac
      301 ggctatggac tcagcgacat gctggcgcgt gccgcgtcgc gcaggcacgg cgcgacggtc
      361 ttcggctatt gggtgcgtga cccgcagcgc tacggggtgg cggaattcga ttcatccggg
      421 cgcgtgatcg acatcgtcga gaagcccgca aagcccgcct ccaacttcgc ggtcgtgggc
      481 ctgtacttct acgatggacg cgctccggag ttcgctgcgt cattgcggcc ctcggctcgc
      541 ggcgaactcg aaatcaccga cctgaaccgc tgttacctcg cggacggctc actgcggatc
      601 gagcaactgg gtcgcggcta cgcgtggctg gacgccggaa ctcacgagtc actgctccag
      661 gcatccggat tcgtcgaaac gctggagatg cgacagggcc tcaggatctg ctgtcccgaa
      721 gaaattgcct ggcgcaacgg ctggatatcg tccgacgaac tggcgaagct cgcgggcacc
      781 atggccggca acagctacgg tcgatacttg ctcgacctga tcgagcagga tccgggcctg
      841 tggcgcggcg caaagagccc ggcctgacgg attgctcgcc tgcgtgtcgg cgccgagcgc
      901 acggtgtttg accgcggcgc actctggcca cgaggagttc agtgcccgcc ggtcacgtca
      961 gttgtcgcag cctcgctcgc cccggcatcg gctgcgggcg gaaagatctg cgtgaacatc
     1021 cctgacagct tcgccgcacc ggtgagcgaa aggtgatcgt catcggagta cagcggtcga
     1081 ccggattcca cgatcgcaca atggcccgcc tggcagagga cttcatccgg cgacaggatt
     1141 ctcagcttcc cggccgcctg cagccgctgg agcaccggcg tcacgtgctg ctgccgcagc
     1201 cggtactcct cgagcgtagg agcgatgctg aatgcccggt cctggcggtg catgcgcgcc
     1261 agcacggacg gcacgttcca gccgacctcg ggtaccgcgg acaccagcca caccgtcttg
     1321 ccgtgcgcac gcagcgaatc caccgtcgtc tcgagcgttc gagcgaagac tctttgattc
     1381 tcggtcatcg acagctcccg ggtctctgcg tcccggaggt agatgctcga tccggagccg
     1441 cgttcgttct tgtagcgggt tccctcggcg taacctgccc atcggccgat gagtatcacg
     1501 tcggtgagcg ccgggtcgtc cgcgatgaat tgcagcaccg cagcggcaaa ttcggggcaa
     1561 cgacgggcct gggggtcagg ctcccggtcg agaccgagca gcgggccgca cccgtttcga
     1621 gtcgcgagca ggcccgctct gcccccgcgc gctgcagcct cgccgacgac gtcggccatc
     1681 gcccgcgcgt gcgagtcgcc ccagatcacg aaactcggaa ggacgtcgcc ggacgcaccg
     1741 acgcggcaca ggtcgcccga cctgacgtcg cggatgttgc ggtttccgca tttccagttg
     1801 cgcggtcgcc ggtcgtcgat gccggccatg agtgacgccg tagtcgggtc cagtcgaccg
     1861 gggaacccgg cgttcgacac cacgaatcgt ccgagaccgg ccgtgacgag catggccgca
     1921 gccgccaccg cgaacagcgc cctgcgggtc agcacgccgc tcttgccccg gaagggccgc
     1981 tcgacatatc gccatgaaag tgctgccgcg acggcagcga tgaccaggac tccggcggcc
     2041 tccggtcgcg tcaggtcacg gatcgtccag tactccgcga ataccagcag cggccagtgc
     2101 cacaggtaca gcgagtacga gatgagtccg atcgccacga agggcttggt ctgcaggatc
     2161 cgccccacga cgttggggcc gcccgtaccg gaccagatga tcagcgccgc gcccacgcag
     2221 ggcacggtgg cggcgatgcc cgggaattga atcgccgcgg aaaaccagcc gactgcgaag
     2281 acgaccaggg ccgcgccgac cagggccgcg gcgttgcgcg tgacacgccg atgcgccctc
     2341 ggaaaggcgc cgagtgccag cagggcaccg agcccgagtt cccacgcccg cgtgtgggcg
     2401 agatagaacg atcggtccgg gtgcgtgggc gtctgggtca cgctgaggta cagcgagccg
     2461 agcacgacca gccagacgaa cagcttgtgc cggccaccca ggtaacgctg gaccgccacc
     2521 aggcacagcg ggaacaggat gtagtactgc tcctcgacgg cgagcgacca ggtgtgcagg
     2581 agtggcttgc tgtgcgcgtc cgtgtcgaaa tagcccgcct gggagttgaa gaagaagttc
     2641 gacgcgaagt acgtggtcgc cacgaggctg cgggcgaacg acttcaggtc ggtcggcagc
     2701 aggagcagca ccgcgacgac ggcagtgaac gccaataccg tgtacagggc cggaaagatc
     2761 cggcggatgc ggcgctcgta gaagctccgt acggagaacg tgccctgcac gacctccggc
     2821 aggatcaggc cggtgatcag gaacccggaa atgacgaaga agatgtcgac accgacgaaa
     2881 ccgccgcgaa atccggcgaa gtcggcgtgg tacagcag
//
LOCUS       DAOMFD010000240         2915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_17779_length_2915_cov_38.823077, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000240 DAOMFD010000000
VERSION     DAOMFD010000240.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2915
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17779_length_2915_cov_38.823077"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="PL152_10340"
     CDS             complement(<1..700)
                     /locus_tag="PL152_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR49725.1"
                     /translation="MGKRALIVDDSRSARVILSRMLEQHGMAVDTAESAEQALEFLQQ
                     HRPDVIFMDHLMPGMDGFQAVQAIKSDPQTATIPLMMYTSQEGEVYVSQARALGAVGV
                     LPKTVRPVDVSRVLYQLHLLPDRRQDRSTLFRRPEPSAAFASPEGAEPPHQGPPPARV
                     ESTASADVAMMSMPVSPGALNEMSAGLRQSVQQLVKDQLAEQRRFMLATFEAFARRLG
                     TEIREQAQKPATPTV"
     gene            862..2910
                     /gene="fusA"
                     /locus_tag="PL152_10345"
     CDS             862..2910
                     /gene="fusA"
                     /locus_tag="PL152_10345"
                     /inference="COORDINATES: protein motif:HMM:NF009381.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HQR49726.1"
                     /translation="MPYSTPDIRNIALVGQAGAGKTLLAEALLAQSGAIRSKGSLARG
                     TTVCDFDPQEKALLHSLDAAICGFETQGKRVNMIDTPGYPDFLGRSLCALEAVETAVI
                     VVSAVNGVEPMTHRMMEFAKARGLCRMIVVNKIDSREARTEQVLEEIRETFGKACLPL
                     NLPAEGGKAVVDCFFQPHGKPTDFSSVEAAHTEIIDQVVEVDEKLMALYLEQGEELSP
                     EQLHDPFEQALREDHLVPICFCSAETGAGVQEFMNVLLRLGPNPAEGNPPPFLKGEGE
                     AAVRVQVTPDPSKHVVAHVFKVTIDPFVGKLGIFRVHQGTVKAGAQLLVGDARKPIKI
                     AHLFQLQGKEHVEVAQAIPGDICAVPKIDELHFDAVLHDSHDEDHHHLKSVTFPPPML
                     GLAIRAEKRGEEQKLSESLHRLVAEDPCVRVEHHTAQNETVLYGLGDLHLRVMLQRMT
                     ERYGVAVKTSPPSVPYRETITRPAEGHHRHKKQTGGAGQFGEVYLRVEPLPRGSGFEF
                     VDDVVGGAIPGQFIPAVEKGVRQVLVDGAIAGFELQDVRVSVYDGKHHAVDSKEVAFV
                     AAGKKAFVAAIKEAGPIVLEPIVKVDINTPAASIGDVTGDLSTRRARISGQDMLPGGR
                     AQITATVPLAELQDYLSRLKALTGGEGTYTMDLSHYDPVPARRQQELVAAFKPQEEAD
                     "
BASE COUNT          492 a          996 c         1006 g          421 t
ORIGIN      
        1 cgaccgtcgg cgtcgccggc ttctgcgcct gctcgcggat ctccgtgccg aggcggcgcg
       61 cgaacgcctc gaacgtcgcc agcatgaagc ggcgctgctc ggccagctgg tccttcacga
      121 gctgctgcac cgattgccgc agcccggcgg acatctcgtt cagggcaccg ggcgacaccg
      181 gcatggacat catggcgacg tccgccgacg cggtgctttc gacgcgggcc ggcggtggac
      241 cctgatgcgg cggctcggca ccctccggcg aggcgaatgc tgccgagggc tcgggacggc
      301 ggaacagcgt cgagcggtcc tggcggcggt ccggcagcag gtgcagctgg tagagcacgc
      361 gggacacgtc cacgggccgc accgtcttcg gcagcacgcc cacggcgccg agcgcccggg
      421 cctggctcac gtagacctcg ccttcctgcg acgtgtacat catgagcggg atggtggcgg
      481 tctgcgggtc gctcttgatg gcctgcacgg cctggaaccc gtccatgccc ggcatcaggt
      541 ggtccatgaa gatcacgtcc gggcggtgct gctgcaggaa ctcgagggcc tgctcggcgg
      601 actcggcggt gtccacggcc atgccatgct gctcgagcat gcggctcagg atcaccctgg
      661 ccgagcgcga gtcgtccacg atcagggcgc gcttgcccat cgtcatgctt catacccccg
      721 gaggacggcc cgaacaccgc cgtgcgtacc ggctttctac cttgaaccgg ggcgtaagcc
      781 aatcccggca aacgcctctg gcctcgggcg gctccggagt tatgcttcgg tcacgcaata
      841 caggaacaac ggaggcagca aatgccctat tcgacacccg acatccgcaa catcgcgctc
      901 gtaggccaag ccggcgcggg caagacgctg ctggccgaag ccttgctcgc gcagtccggt
      961 gcgatccggt ccaagggttc tctcgcgcgc gggacgaccg tgtgcgactt cgacccgcag
     1021 gaaaaggccc tgctgcactc gctcgacgcc gccatctgcg gcttcgagac ccagggcaag
     1081 cgcgtcaaca tgatcgacac gccggggtac ccggacttcc tcggccgcag cctgtgcgcg
     1141 ctggaggcgg tcgagaccgc cgtgatcgtc gtgagcgcgg tgaacggcgt cgagccgatg
     1201 acccaccgca tgatggagtt cgcgaaggcg cgcgggctgt gccgcatgat cgtcgtgaac
     1261 aagatcgaca gccgcgaggc acgcaccgag caggtcctcg aggagatccg cgagaccttc
     1321 ggcaaggcgt gcctgccgct caacctgcca gcggagggag gaaaggcggt agtcgactgc
     1381 ttcttccagc cgcacggcaa gccgacggat ttctcgtcgg tcgaggcggc ccacaccgag
     1441 atcatcgacc aggtggtcga ggtcgacgag aagctcatgg cgctctacct cgagcagggc
     1501 gaggaactct cgcccgagca actgcacgac ccgttcgagc aggcgctgcg cgaggaccac
     1561 ctcgtgccga tctgcttctg ctcggccgag accggtgccg gcgtccagga attcatgaac
     1621 gtcctgctgc ggctgggacc caaccccgcg gagggcaacc cgccgccgtt cctcaagggc
     1681 gaaggcgagg ctgcggtgcg ggtgcaggtc acgccggacc cgtcgaagca cgtcgtggcg
     1741 cacgtgttca aggtcacgat cgacccgttc gtgggcaagc tcggcatctt ccgcgtccac
     1801 cagggcacgg tgaaggccgg cgcccagctg ctcgtcggcg acgcgcgcaa gcccatcaag
     1861 atcgcgcacc tgttccagct gcagggcaag gaacacgtgg aggtggcgca ggccattccg
     1921 ggcgacatct gcgccgtgcc caagatcgac gagctgcact tcgacgcggt gctgcacgat
     1981 tcgcacgacg aggaccacca tcacctgaag tcggtgacgt tcccgccgcc gatgctgggc
     2041 ctggcgatcc gcgccgagaa gcgcggcgag gagcagaagc tctcggagtc cctgcaccgc
     2101 ctcgtcgccg aggatccgtg cgtgcgcgtc gagcaccaca ccgcccagaa cgagaccgtg
     2161 ctctacgggc tcggcgacct gcacctgcgc gtgatgctgc agcggatgac cgagcgctac
     2221 ggcgtggccg tgaagaccag cccgccgagc gtgccgtacc gcgagaccat cacccggccg
     2281 gccgagggtc accaccgcca caagaagcag accggcggcg ccgggcagtt cggcgaggtg
     2341 tacctgcggg tcgagccgct gccgcgcggc tccggcttcg agttcgtgga cgacgtggtg
     2401 ggcggggcga tcccgggcca gttcatccca gccgtggaga aaggcgtccg gcaggtgctc
     2461 gtggacggcg cgatcgccgg cttcgagctg caggacgtgc gcgtgagcgt gtacgacggc
     2521 aagcaccacg cggtcgattc aaaggaagtc gcgttcgtcg ccgccggcaa gaaggcattc
     2581 gtcgcggcga tcaaggaggc aggcccgatc gtgctcgagc cgatcgtcaa ggtcgacatc
     2641 aacacgccgg ccgcctcgat cggcgatgtg acgggcgacc tgtcgacgcg ccgtgcccgc
     2701 atcagcgggc aggacatgct gccgggcggc cgcgcgcaga tcacggcgac cgtgccgctc
     2761 gccgagctgc aggactacct gtctcgcctg aaggcgctca ccgggggtga aggcacctac
     2821 acgatggacc tgagccacta cgacccggtt cccgcccgca ggcagcagga actggtcgca
     2881 gcgttcaagc cgcaggagga agccgactag ttctt
//
LOCUS       DAOMFD010000241         2885 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_18145_length_2885_cov_34.523322, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000241 DAOMFD010000000
VERSION     DAOMFD010000241.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2885)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2885
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18145_length_2885_cov_34.523322"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..792
                     /locus_tag="PL152_10350"
     CDS             <1..792
                     /locus_tag="PL152_10350"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HQR49727.1"
                     /translation="FHNFVLSRYFSAEPVEFERVLEDLVAQGERVRPLVDDITERLRV
                     EMQRGGNLLFEGAQGALLDIDVGTYPFVTSSNTIAGAAAVGTGLGPRDIHRVLGIVKA
                     YTTRVGAGPFPTELFDVTGEHLSRVGHEFGAVTGRPRRCGWFDAVALRRAVLHNSLSS
                     LGVTKLDVLDGLDTLQIATGYRIDGQVREQPPLLSHRYSECEPVYESLPGWSEPTSGI
                     TDFDKLPANARKYLARIEQLAGVPIDIVSTGPDRAETIVLRHPFE"
     gene            815..1960
                     /locus_tag="PL152_10355"
     CDS             815..1960
                     /locus_tag="PL152_10355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR49728.1"
                     /translation="MNAQESPWMDDDLRMLREAVSRYVETEMLPNEPRWRAQHHVDRE
                     TWRAVGEAGFLLMDVPAEYGGGGGDFRHEAVLYEELARRGISGFGQGVHSIAAHYVLN
                     YGTEEQKQRWLPRMARGELIGAIAMTEPGAGSDLKGIRTRAVREGGDYVINGSKIFIT
                     NGSIATILMLVVRTDPTDRSRGLSILMVETENLPGYRVGRVLDKMGMTAQDTSELYFE
                     DVRVPADCLLGGVEGEGMHQLMGDLPYERLTIAVGGVGMMEGAVAETIRYVNERKAFG
                     QSISAFQNTRFKLAEIVTKTRVARTFVDRCIAQLVEGKLDTETAAMAKWWITDTQQEV
                     IDECVQLHGGYGYMNEYLVCRMFADSRVQRIYGGTNEIMKELISRAI"
     gene            complement(2034..2118)
                     /locus_tag="PL152_10360"
     tRNA            complement(2034..2118)
                     /locus_tag="PL152_10360"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2082..2084),aa:Leu,seq:cag)
     gene            2323..>2885
                     /locus_tag="PL152_10365"
     CDS             2323..>2885
                     /locus_tag="PL152_10365"
                     /inference="COORDINATES: protein motif:HMM:NF019813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HQR49729.1"
                     /translation="MLEALRRAGRPLAQDEIVAALGPVAGASLETQLAGLVKRGEAVQ
                     NRRGQYCLREHIPGLTVGTVQGHRNGDGWLMPDDATAPVYLPPQQMREVMHGDRVAVR
                     VEGPRFRGKPQGTIVEVLERRTKEVVGRLQVESGVAYLTPDNTRIAHRVLVPPSHMGA
                     AQSGQVVIVELTQQPGRGAQPVGRVARV"
BASE COUNT          491 a          959 c         1023 g          412 t
ORIGIN      
        1 ttccacaact tcgtgctgag ccgctatttc agcgcggagc ccgtcgagtt cgagcgcgtg
       61 ctcgaggacc tcgtcgcgca gggcgagcgc gtccggccgc tcgtggacga catcaccgag
      121 cgcctgcgtg tcgagatgca gcgcggcggc aacctgctgt tcgagggtgc gcagggtgcg
      181 ctgctcgaca tcgacgtcgg cacgtacccg ttcgtgacgt cgtcgaacac gatcgcgggc
      241 gcagcggccg tcggtaccgg cctcgggccg cgcgacatcc accgggtgtt gggcatcgtc
      301 aaggcctaca cgacgcgcgt cggcgcgggg ccattcccga cggagctctt cgacgtgacc
      361 ggcgagcacc tgtcgcgggt gggccacgag ttcggtgccg tcaccgggcg tccgcgccgc
      421 tgcggctggt tcgacgcggt cgccctgcgc cgggccgtgc tgcacaacag cctctcgagc
      481 ctcggcgtca ccaagctcga cgtgctcgac ggcctcgaca cgctgcagat cgcgaccggc
      541 taccggatcg acgggcaggt gcgcgagcag ccgcccctgc tgtcgcatcg gtattccgag
      601 tgcgagccgg tgtacgaatc gctgcccggc tggtcggaac cgacgagcgg catcacggac
      661 ttcgacaagc tgccggcgaa cgcgcgcaag tacctcgcgc gcatcgagca gctggccggc
      721 gtgccgatcg acatcgtgtc gaccggcccc gaccgggccg agaccatcgt gctcaggcac
      781 cccttcgaat gaccgcgcgg agagcaggac agtgatgaac gcccaggaat cgccttggat
      841 ggacgacgat ctgcgcatgc tgcgcgaggc cgtgtcgcgc tacgtggaga cggagatgct
      901 gccgaacgag ccgcgctggc gggcgcagca ccacgtggat cgcgagacgt ggcgcgcggt
      961 gggcgaggcg ggattcctgc tgatggacgt gccggccgaa tacggcggcg gtggcggtga
     1021 cttccgccac gaggcagtgc tgtacgaaga actcgcgcga cgcggcatct cgggtttcgg
     1081 ccagggcgtg cacagcatcg cggcccacta cgtcctcaac tacggcaccg aggagcagaa
     1141 gcagcgctgg ctgccgcgca tggcgcgcgg cgaactgatc ggcgcgatcg ccatgaccga
     1201 gccgggcgcg ggctccgacc tgaagggaat ccgcacccgg gccgtgcgcg aaggcggcga
     1261 ctacgtcatc aacggctcca agatcttcat caccaacggc tcgatcgcga cgatcctgat
     1321 gctggtcgtg cgcaccgatc ccacggaccg gtcgcgcggc ctctcgatcc tgatggtcga
     1381 gacggagaac ctgcccggtt accgcgtcgg tcgcgtgctc gacaagatgg gcatgaccgc
     1441 gcaggacacc tcggagctct acttcgagga cgtgcgcgtt cccgccgact gcctgctcgg
     1501 cggtgtcgag ggcgagggca tgcaccagct gatgggcgac cttccgtacg agcggctgac
     1561 gatcgcggtc ggcggcgtcg gcatgatgga aggcgcggtc gcggagacca tccgctacgt
     1621 gaacgagcgc aaggcgttcg ggcagtcgat ctcggcgttc cagaacacgc ggttcaagct
     1681 cgccgagatc gtcaccaaga cgcgcgtcgc ccgcaccttc gtggaccggt gcatcgcgca
     1741 gctggtggag gggaagctcg acaccgagac cgctgcgatg gcgaagtggt ggatcaccga
     1801 cacgcaacag gaggtgatcg acgagtgcgt ccagctgcac ggcggctacg gctacatgaa
     1861 cgagtacctc gtgtgccgca tgttcgccga ctcgcgcgtg cagcggatct acggcggcac
     1921 caacgagatc atgaaggaac tgatctcgcg cgcgatctga gcgagggcga caaaagccca
     1981 ggggaaacag acagttgcgg cgcgagatgc tagagtcgca acgccgccgc agatggtgcc
     2041 caggagagga ctcgaacctc cacggtgtta cccgctagta cctgaaacta gtgcgtctac
     2101 caattccgcc acctgggcat cggttgcggc tcggcacggg ggccggctgg aaggtggcgc
     2161 aatttactgg cggccctcgg ggctgtcaac gcatgggctg gccgtaagcc gcaatcccgg
     2221 acactgatgc agagaaatcg aaaacgtcgc tcgagccaat cgccacgcgg cccggcaagg
     2281 ccgcgtccac cccaacgaac gtcgccggcg cctggcgcgg ccgtgctcga agccttgagg
     2341 cgcgcaggcc ggccgctcgc gcaggacgaa atcgtcgccg cactcgggcc ggtggccggg
     2401 gccagcctcg agacacaact cgccggactg gtgaagcgcg gcgaggccgt gcagaaccgg
     2461 cgcggccagt attgcctgcg cgagcacata cccggcctca cggtgggcac cgtgcagggg
     2521 caccgcaatg gcgacgggtg gctgatgccc gacgacgcga cggcacccgt gtacctgccg
     2581 ccgcagcaga tgcgggaggt catgcacggc gaccgcgtgg ccgtgcgggt cgaggggccg
     2641 cgattccgtg gcaagcccca gggtacgatc gtcgaagtcc ttgagcggcg cacgaaggag
     2701 gtcgtggggc gcctgcaggt cgaatccggc gtggcgtacc tcactcccga caacacgcgg
     2761 atcgcccatc gcgtcctcgt gccgccgtcg cacatggggg cggcgcaatc cgggcaggtg
     2821 gtcatcgtcg aactcaccca gcagcccggt cgcggcgcgc agcccgtcgg ccgggtcgcg
     2881 cgtgt
//
LOCUS       DAOMFD010000242         2878 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_18219_length_2878_cov_33.552249, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000242 DAOMFD010000000
VERSION     DAOMFD010000242.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18219_length_2878_cov_33.552249"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..953)
                     /locus_tag="PL152_10370"
     CDS             complement(<1..953)
                     /locus_tag="PL152_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQR49730.1"
                     /translation="MSSLRDEVARRRTFAIISHPDAGKTTLTEKLLLFGGAIQMAGTV
                     KGRKAARHATSDWMALEQQRGISVTSSVMQFPYQGRMVNLLDTPGHEDFSEDTYRTLT
                     AVDSALMVIDCAKGVEERTIKLMEVCRLRDTPIMTFINKLDREGREPLELLDEIEKVL
                     RITCAPITWPIGMGRELKGVYHLLEDRIYVYHPGEKGRAGTHATIHGLTSAAATEFLG
                     DRAAAFRDEVELVHGASHPFSRDEYLAARQTPVFFGSAINNFGVTELLSAFVEQAPSP
                     RPRATTSRQVDAFEQPLTGFVFKIQANMDPGHRDRIAFMRI"
     gene            complement(994..1506)
                     /gene="rimI"
                     /locus_tag="PL152_10375"
     CDS             complement(994..1506)
                     /gene="rimI"
                     /locus_tag="PL152_10375"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HQR49731.1"
                     /translation="MSAASADTGGHRHRLVKFRAMTPADVSAVAALEHAAYPFPWSEG
                     IFRDCLRVGYLCRVAEVEGEVVAYGIVAMGAGEAHILNLCVRGDLRGRSIGRQMLLLL
                     LERARQAGMADAFLEVRPSNRLAITLYQSVGFAQVGLRKGYYQAEDGREDALVLKLAL
                     GDPDGGALGP"
     gene            complement(1503..2150)
                     /locus_tag="PL152_10380"
     CDS             complement(1503..2150)
                     /locus_tag="PL152_10380"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HQR49732.1"
                     /translation="MPAPAETQTVAAPLATAPCDVARLGWDALRETVAACTRCELHAT
                     RTQTVFGVGSTAARWMFIGEAPGADEDRQGEPFVGRAGQLLTSMIRALGLERGDVYIA
                     NVLKCRPPGNRDPRPDEARACRDYLGRQVELVDPSIIVAVGRIAAQNLLETDTPLAKL
                     RGRVHALGPARRPLVVIYHPAYLLRSPGEKRKAWLDLLFARQVFQRLERGESVLP"
     gene            complement(2264..>2878)
                     /locus_tag="PL152_10385"
     CDS             complement(2264..>2878)
                     /locus_tag="PL152_10385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine/phosphatidylserine synthase"
                     /protein_id="HQR49733.1"
                     /translation="GRIARWTNTQSEFGKEYDSLSDMVAFGLAPAVVAYQWGVARIAE
                     YGAVWGRLGWLATFFYAACAAIRLARFNARSGIADKRYFEGLPSPSAAALLAGFVWML
                     NDLQREGLRALVLAFVVATLAGSLMVSRFPYVSGKDFNLNQRVPFALLVLVPLGFIVV
                     GSSPPEMLFGLFGLYALSGPLLWAWHRAARGRRGATHGGAGHRK"
BASE COUNT          440 a         1018 c         1002 g          418 t
ORIGIN      
        1 cagatgcgca tgaacgcgat gcggtcgcga tggccgggat ccatgttggc ctggatcttg
       61 aatacgaagc cggtcagcgg ctgctcgaat gcatccacct gccgcgaggt cgttgcacgg
      121 ggccgcggcg acggcgcctg ctcgacgaac gcggacagca gctccgtcac gccgaagttg
      181 ttgatcgccg agccgaagaa caccggcgtc tggcgcgcgg cgaggtactc gtcgcgcgag
      241 aacgggtgtg acgccccgtg caccagctcg acctcgtcgc ggaaggccgc cgcgcggtct
      301 cccaggaatt ccgtcgccgc cgcgctcgtg agcccgtgga tggtcgcatg cgtaccggcg
      361 cggcccttct cgccggggtg gtacacgtag atgcggtcct cgagcaggtg gtagacgccc
      421 ttcagttccc gccccatgcc gatcggccac gtgatcggcg cgcaggtgat ccggagcacc
      481 ttctcgatct cgtcgagcag ctcgagcggc tcgcggccct cgcggtcgag cttgttgatg
      541 aaggtcatga tcggcgtgtc gcgcagccgg cagacctcca tgagcttgat ggtgcgttcc
      601 tcgacgccct tcgcgcagtc gatgaccatc agcgcggagt ccacggcggt cagcgtgcgg
      661 taggtgtcct cggagaaatc ctcgtggccc ggcgtgtcga gcaggttgac catccggccc
      721 tggtacggga actgcatgac gctcgaggtg accgagatgc cgcgctgctg ctccagcgcc
      781 atccagtccg aggtcgcgtg tcgcgcggcc ttgcgtccct tgacggtgcc cgccatctgg
      841 atcgcgccgc cgaacagcag cagcttctcg gtgagcgtgg tcttgccggc gtcggggtgg
      901 gagatgatgg cgaaggtgcg gcggcgcgcc acttcgtcgc gcagggaact catcggtcgg
      961 ggcgcgtccg tcggaatcgg ggccgcgatt ctatcaggga ccaagtgcac cgccgtccgg
     1021 atcgcccagc gcgagcttga ggaccagcgc gtcctcgcgg ccgtcctccg cctggtagta
     1081 gcccttgcgc agaccgacct gcgcgaatcc caccgactgg tacagcgtga tcgccaggcg
     1141 gttcgagggc cgcacctcga ggaacgcatc ggccatgccc gcctggcgcg cgcgctcgag
     1201 cagcagcagc agcatctggc ggccgatgct gcgcccgcgc aggtcgccgc gcacgcacag
     1261 gttgaggatg tgggcttcgc ccgcgcccat cgcaacgatc ccgtaggcga cgacctcccc
     1321 ctcgacttcg gcgacccggc acaggtagcc gacgcgcagg cagtcgcgga agatgccttc
     1381 actccacgga aacgggtagg ccgcgtgctc gagcgcagcg accgcggaca cgtccgccgg
     1441 ggtcatcgcg cggaatttca ccaggcgatg gcggtggccg cccgtgtcgg cgctcgcggc
     1501 gctcacggca gcaccgattc tccccgctcg aggcgctgga acacctgccg cgcgaacagc
     1561 aggtcgagcc acgccttgcg cttctcgccg gggctgcgca acaggtacgc gggatggtag
     1621 atcacgacca gcggtcgccg ggccggcccg agcgcgtgca cgcgtccccg cagtttcgcg
     1681 aggggcgtgt ccgtttcgag caggttctgc gcggcgatgc gcccgacggc gacgatgatg
     1741 gacgggtcga cgagttcgac ctgccgcccg aggtagtccc ggcaggcgcg ggcctcgtcg
     1801 ggacgcgggt cgcgattgcc gggcggccgg cacttgagta cgttcgcgat gtacacgtcg
     1861 cctcgttcga ggccgagcgc ccggatcatc gaggtcagga gctggccggc gcgaccgacg
     1921 aagggctccc cttgccggtc ctcgtcggca cccggtgcct cgccgatgaa catccatcgg
     1981 gcggcggtgc ttccgacacc gaacaccgtc tgcgtgcgcg tcgcgtgcag ctcgcaccgc
     2041 gtgcaggcag ccaccgtctc acgcagcgcg tcccacccga ggcgggcaac gtcgcaaggt
     2101 gccgtcgcca gcggggcagc caccgtctga gtctcggccg gcgcgggcat tgcgacctcg
     2161 gccggacgga ccggccgaag cgcaggctgg ccgcgccgga cgtacacgtc cacccccaac
     2221 gcctcgagat acgccgcacg ccgtggatcc accgcggcgc cctttatttc cggtggcctg
     2281 cgccgccgtg ggtcgcgccg cgacgtccgc gggcggcgcg gtgccacgcc cagagcagag
     2341 gcccggacag cgcgtacagc ccgaacagcc cgaacagcat ctcgggcggg ctcgacccca
     2401 ccacgatgaa gccgagcggc acgagcacca gcagcgcgaa cgggacgcgc tggttcaggt
     2461 tgaagtcctt gccgctcacg tacgggaatc ggctgaccat caggctgccg gccaacgtcg
     2521 cgacgacgaa ggcgagcacc agtgcccgca gcccttcgcg ctgcaggtcg ttcagcatcc
     2581 acacgaaccc ggcgagcagg gccgcggccg acgggctcgg caagccctcg aaatagcgct
     2641 tgtcggctat gcccgagcgc gcattgaagc gcgccaggcg gatcgcggcg cacgcggcat
     2701 agaagaacgt ggcgagccag ccgagccgac cccacacggc gccgtactcc gcgatgcgcg
     2761 ccacgcccca ctggtaggcg accacggccg gcgcgagccc gaatgcgacc atgtccgaca
     2821 ggctgtcgta ctccttgccg aattcgctct gcgtattggt ccagcgcgcg atgcggcc
//
LOCUS       DAOMFD010000243         2877 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_18229_length_2877_cov_40.408575, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000243 DAOMFD010000000
VERSION     DAOMFD010000243.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18229_length_2877_cov_40.408575"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="PL152_10390"
     CDS             complement(<1..545)
                     /locus_tag="PL152_10390"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HQR49734.1"
                     /translation="MTHVAPVRLSFLFAALLALAVPALADAPAVGSPAPAFKLQDQDG
                     KWHSLSDYRGKWVVLYFYPKDDTPGCTTEACAFRDNIFAFDKEGAVVLGISVDEVGSH
                     KAFAEKHSLPFTLLADPTKAVTKEYGVLRTYMAVMEMAHRETFIIDPQGRIAKHYDTV
                     DPDSHSKVVLDDLKALKAAYKP"
     gene            629..1786
                     /locus_tag="PL152_10395"
     CDS             629..1786
                     /locus_tag="PL152_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HQR49735.1"
                     /translation="MQDSDTETNYVFVENREALVLAGGGARAAYQVGVLRAMADWLPE
                     GAACPFEVLVGTSAGALNAATIVSRAARFRDGVATLERVWSGFHVGQVVRVDPATMLR
                     SSLHWILSVVFGGWLLPPPRSLLDTTPLRDLLMRTIPLDGITDAVRAGLVRALAVATT
                     SYSTGKAVAFFDAEPTVAQWSRVRRNGVRRRIDIEVLMASAAIPYIFPAARVDGDYYG
                     DGAMRQLAPLSPAVHLGANRVLVVGTRTDAAASPADGALDRPPSPGHLLGFVLDSLFT
                     DGLSIDLERLRQINALLQSEGLRRDHERRPIEALVIQPSDDPTAIARRHVRAMPRTLR
                     TLLRTIGALEARGGLLASYLLFESDYTTELMRLGRADAEARRSEIETYLMP"
     gene            complement(1803..2618)
                     /locus_tag="PL152_10400"
     CDS             complement(1803..2618)
                     /locus_tag="PL152_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR49736.1"
                     /translation="MSFSSRRLTVDGVELSVVVEGEGPDVLLVHGFPDDHGVWRGQIP
                     ALVAAGYRVIAPDTRGCGESGVPPRVADYSVDRLVADLVGVLDALGVARARVVGHDWG
                     ALQGWYLAMRHPERVERYVALSVGHPAAYAHAGIAQKLKAYYAVLIQFRHVVEFVVTR
                     FDWLLFRLFSGHSAEFPRWRLQLSRPGRLTAGFNYYRANLRRVLSQKDGSVTIPVVGI
                     WSSRDRFLTERQMVQSARYCDAGWTYVRVDGLGHWMQLDDPDRVNALLLQHLE"
     gene            complement(2647..>2877)
                     /locus_tag="PL152_10405"
     CDS             complement(2647..>2877)
                     /locus_tag="PL152_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HQR49737.1"
                     /translation="VAVVALAWFRNWKIAAVIFAAMVINLLAAALAGVFVPLTMKRLG
                     VDPAVAGGVVVTTVTDVVGFASLLGLGTLVLL"
BASE COUNT          450 a         1001 c          986 g          440 t
ORIGIN      
        1 ggcttgtacg cagccttgag cgccttgagg tcgtcgagca cgaccttgga gtggctgtcc
       61 ggatccaccg tgtcgtagtg cttcgcgatg cgcccctgcg ggtcgatgat gaacgtctcg
      121 cggtgagcca tctccatgac ggccatgtag gtcctgagca cgccgtactc cttggtcacg
      181 gccttggtcg gatcggccag cagcgtgaac ggcaggctgt gcttttccgc gaacgccttg
      241 tgcgagccca cttcgtcgac gctgatgccg agcaccaccg cgccttcctt gtcgaacgcg
      301 aagatgttgt cgcggaacgc gcaggcctcc gtcgtgcaac cgggcgtgtc gtccttggga
      361 tagaagtaca gcaccaccca cttgccgcgg tagtcggaca ggctgtgcca cttgccgtcc
      421 tggtcctgca gcttgaaggc cggcgccggg ctgcccacgg cgggcgcgtc cgcgagtgcc
      481 gggactgcca gcgcgaggag ggcggcgaac agaaatgaga gtcggacagg cgcgacgtgc
      541 gtcataggat tctcccgtgt gggcggaccg tcggcccgcg cgtgccatac taatcccggg
      601 ccccgaccct gtcagcgagg atcgcccctt gcaggactcg gacaccgaaa cgaactacgt
      661 gttcgtcgag aatcgcgagg cgctggtgct cgcgggcggc ggcgcccgtg ccgcttacca
      721 ggtgggcgtg ctccgtgcga tggccgactg gctgccggaa ggcgcggcct gccctttcga
      781 ggtcctggtc ggcacgtcgg ccggcgccct gaacgcggcg accatcgtgt cgcgggccgc
      841 gcgattccgc gacggcgtgg ccacgctgga acgcgtctgg tcgggattcc acgtcgggca
      901 ggtcgtgcgg gtcgatcccg ccacgatgct gcggtcgagc ctgcactgga tcctctcggt
      961 ggtcttcggc ggatggctgc tgccgccacc gcgatcgctg ctcgacacga cgccgttgcg
     1021 cgacctgctc atgcgcacga tcccgctgga tgggatcacc gacgcggttc gggcgggcct
     1081 ggtccgggcg ctcgccgtcg cgacgaccag ttactcgacg ggcaaggcgg tcgcattctt
     1141 cgacgccgag cctaccgtcg cacagtggtc gcgggtccgc cgcaacggtg tgcgtcgtcg
     1201 catcgacatc gaggtgctga tggccagcgc cgcgattccc tacatctttc cggcggcccg
     1261 cgtcgatggc gactactacg gcgacggcgc catgcgccag ctcgctcccc tgagccccgc
     1321 ggtccacctc ggtgcgaatc gcgtgctggt cgtcgggacg cgcaccgatg cggctgcgtc
     1381 gcccgcggac ggcgcgctgg atcggccacc gagcccgggc cacctgctcg gcttcgtgct
     1441 cgattcgctg ttcaccgacg gcctgtcgat cgatctcgag cggctgcggc agatcaacgc
     1501 gctcctgcag tcggagggct tgcgccgcga ccatgaacgc cggccgatcg aggcgctggt
     1561 gatccagccg agcgacgacc cgacggcgat cgcccgcagg cacgtgcgcg cgatgccgcg
     1621 gaccctgcgc acgctgctac ggacgatcgg agcgctcgag gcgcgcggcg gcctgctcgc
     1681 gagctacctg ctgttcgaat ccgactacac gacggaactc atgcggctcg gtcgcgcgga
     1741 cgccgaagcg cgccgctccg agatcgagac ctacctgatg ccctagaacg gggcggcacg
     1801 catcactcca ggtgctggag cagcagcgca ttgacgcggt ccgggtcatc gagctgcatc
     1861 cagtgcccga gcccgtcgac tcggacgtac gtccagcccg cgtcgcagta tcgcgcggac
     1921 tgcaccatct gccgctccgt caggaaccgg tcgcgactgc tccagatgcc gacgaccgga
     1981 atcgtcacgc tcccgtcttt ctgcgacagg actcgccgca ggttcgcccg gtagtagttg
     2041 aatccggcgg tcaggcgccc aggccgcgac aactgcagac gccaccgcgg gaactcggca
     2101 gaatgtcccg agaatagcct gaagagcagc cagtcgaatc tcgtgacgac gaactcgacc
     2161 acgtggcgga actggatcag gactgcatag taagccttga gcttctgcgc gatgccggcg
     2221 tgggcgtagg cggccggatg gccgaccgac agggcgacgt agcgctcgac gcgctcggga
     2281 tggcgcatgg cgaggtacca gccctgcagc gcaccccagt cgtgcccgac cacgcgagcc
     2341 ctggcgacgc cgagcgcgtc gagcacgccg acgagatctg cgacgagccg atcgaccgag
     2401 tagtcggcca cgcgcggcgg caccccgctc tcgccgcagc cccgggtgtc gggcgcgatc
     2461 acgcgatagc cggcggcgac gagggcggga atctgtccgc gccacacgcc gtggtcgtcc
     2521 ggaaacccgt gcacgagcag gacgtcgggt ccctcgccct cgacgacgac gctgagttcc
     2581 actccgtcga cggtcaggcg tcgcgacgag aacgacatgc tggggatcct cggtggcggc
     2641 ggacgctcac agcagcacga gcgtccccag gccgagcaac gacgcgaaac cgaccacgtc
     2701 ggtgaccgtc gtgacgacca cgccgccggc gaccgcagga tccacgccga gccgtttcat
     2761 cgtcagcggc acgaagacgc cggcgagcgc cgccgccagc aggttgatga ccatcgcggc
     2821 gaagatcacg gctgcgatct tccagttgcg gaaccaggcc agcgcgacga ccgcgac
//
LOCUS       DAOMFD010000244         2872 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_18288_length_2872_cov_32.050763, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000244 DAOMFD010000000
VERSION     DAOMFD010000244.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18288_length_2872_cov_32.050763"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1094
                     /locus_tag="PL152_10410"
     CDS             <1..1094
                     /locus_tag="PL152_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49738.1"
                     /translation="DLVMIDPVGTGLSRVVGKGETKDFWGVDQDIASVSDFIVRYLGD
                     YQRWSSPKFLLGESYGGMRSGGVAYRLLDHHSVSLNGVILVSPYLDLGSGWAGLQLDI
                     PYANFLPTYAATAWFHNALPNRPAELQPFLREVEAFADGPYLSTLVKGSRATTGERQA
                     VLASLERYTGVSASYWDDANLRLDEPHFLQELLRKQGIAVGRIDTRYSGGDLSRLYAV
                     TKYDPYDAAIGPAIVATFNDYYRKELEVASDREYVLSADIEKDWDNRHKQPDIDYTVP
                     FPNTAVDLAHAMTMNPRMKVLVQQGYFDLACPYRTVEYAIDHLDVPPSVRSNLSIQYY
                     DAGHMMYVNPSSMQKFKRTLAEFIDANGR"
     gene            complement(1115..2497)
                     /gene="mnmE"
                     /locus_tag="PL152_10415"
     CDS             complement(1115..2497)
                     /gene="mnmE"
                     /locus_tag="PL152_10415"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006461364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HQR49739.1"
                     /translation="MSPDSDTIAAIATPAGRGGIGIVRVSGPGCRAIAESLTGGIPAP
                     RIATLREFVGADGEPIDAGLALYFPAPHSLTGEDVLELQGHGGPVVLDVLLGRVLALG
                     ARPAEPGEFTQRAFLNEKMDLAQAEAVADLVDSGSAAAARAAVRSLRGEFSMKVHELA
                     EAMLELRMWVEAAIDFPEEEIDFLANRALGERMDGIRRRFTELAERARQGSILRDGLT
                     LVIAGRPNAGKSSLLNRLAGYDAAIVTATPGTTRDVLRERIEIDGLPLHVLDTAGLRE
                     SADEVEAEGIRRAHREIQQADRVLFVVDASDATAIAALDADLSALPTHAPRTVVFNKI
                     DRISGGARIETGAEAPGRNGLAGQERIYLSATTGAGLDLLRGRLKEYVGFHPEASGAL
                     SARARHLDALRRARAHVEEAHRLLTARHAGELVAQELTDAQRDLGEITGEVTSDDLLG
                     RIFGSFCIGK"
     gene            complement(2579..>2872)
                     /locus_tag="PL152_10420"
     CDS             complement(2579..>2872)
                     /locus_tag="PL152_10420"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HQR49740.1"
                     /translation="MRQAPFMLWIQDLSSKDPYFILPAIMGAAMYGQFKLNPAPPDPM
                     QAKVFAFMPLVMTVMMAWFPSGLVLYWITNTVLSIAQQWNINRLVAAEAKKPG"
BASE COUNT          440 a         1012 c          951 g          469 t
ORIGIN      
        1 ccgacctcgt catgatcgac ccggtcggca ccggcctgtc gcgagtcgtc ggcaagggcg
       61 agacgaagga tttctggggc gtcgaccagg acatcgcctc ggtgtcggat ttcatcgtgc
      121 gttacctggg tgactaccag cgctggtcgt cgcccaagtt cctgctcggc gagagttacg
      181 gcggcatgcg ctcgggcggc gtcgcctacc gtctgctcga ccaccacagc gtgtcgctca
      241 atggcgtcat tctcgtctcg ccctacctcg acctggggtc cggctgggcc ggcctgcagc
      301 tcgacattcc gtacgccaac ttccttccca cctatgccgc cacggcctgg ttccacaacg
      361 cattgccgaa ccggccggcc gagctgcagc cgttcctgcg cgaggtcgag gccttcgccg
      421 acgggccgta cctgtcgacg ctggtcaagg gctcgcgtgc gaccaccggc gagcgccagg
      481 cggtgctcgc ctcgctcgag cggtacacgg gagtcagcgc gtcgtactgg gacgatgcca
      541 acctgcggct cgacgagccg catttcctgc aggaactgct gcgcaagcag ggcattgcgg
      601 tcgggcgcat cgacacccgc tacagcggcg gcgacctgag ccggctgtac gcggtgacca
      661 agtacgaccc ttacgatgcg gcgatcgggc cagcgatcgt cgcgaccttc aacgactact
      721 accgcaagga gctcgaggtc gcctccgacc gcgagtacgt gctgtcggcc gacatcgaga
      781 aggactggga caaccgccac aagcagcccg acatcgacta cacggtgccg ttccccaaca
      841 ccgccgtcga cctcgcgcac gccatgacga tgaacccgcg catgaaggtg ctggtgcagc
      901 agggctactt cgacctcgcc tgcccctacc gcaccgtcga gtacgccatc gaccacctcg
      961 acgtgccgcc gtcggtgcgg tccaacctca gcatccagta ctacgatgcc gggcacatga
     1021 tgtacgtgaa cccgtcgtcg atgcagaagt tcaagcgcac gcttgcggag ttcatcgacg
     1081 cgaacggccg ctgaccgccc ggtcggagcg tcggctactt gccgatgcag aagctgccga
     1141 agatccgccc gagcaggtcg tcgctggtga cctcgccggt gatctcgccc aggtcccgct
     1201 gcgcgtcggt gagttcctgc gccaccagtt ccccggcgtg cctggccgtg agcagccggt
     1261 gcgcttcctc gacgtgtgcc ctcgcccgcc tcagcgcatc gaggtgccgc gcgcgggccg
     1321 agagcgcgcc ggacgcctcc ggatggaagc cgacgtactc tttcaggcgg ccgcgcagca
     1381 ggtccagccc ggcgccggtc gtggcgctca ggtagatccg ttcctggcct gcgaggccgt
     1441 tgcgaccggg cgcctcggcc ccggtctcga tccgtgcccc gccgctgatc cgatcgatct
     1501 tgttgaacac cacggtgcga ggtgcgtgcg tcggcagtgc cgaaaggtcc gcgtccaggg
     1561 ctgcgatcgc cgtggcatcg gaggcgtcga cgacgaacag cacccggtcc gcctgctgga
     1621 tctcgcggtg ggcgcgccgg atgccctcgg cctcgacctc gtcggccgat tcgcgcaggc
     1681 ccgcggtgtc gaggacgtgc aggggcaggc cgtcgatctc gatgcgctcg cgcagcacgt
     1741 cgcgggtcgt cccgggcgtc gccgtgacga tcgcggcgtc gtagccggcg aggcgattga
     1801 gcaggctcga cttgccggcg ttcggccgtc cggcgatcac gagcgtgagg ccgtcgcgca
     1861 ggatcgagcc ttgcctcgct cgttccgcca gctccgtgaa ccgccgccgg atgccgtcca
     1921 tgcgctcgcc gagtgcgcgg tttgcgagaa agtcgatttc ctcctccggg aagtcgatgg
     1981 ccgcctcgac ccacatgcgc agctcgagca tcgcctcggc gagttcgtgc accttcatcg
     2041 agaactcgcc gcgcagcgac cgcaccgcgg ctcgcgcggc cgcggccgag ccgctgtcga
     2101 cgaggtcggc gactgcctcg gcctgggcga ggtccatctt ttcgttgagg aacgcgcgtt
     2161 gcgtgaactc gccgggctcg gccggccgcg cgccgagcgc cagtactcgc ccgagcagca
     2221 cgtcgagcac gaccggaccg ccgtgcccct gcagctcgag cacgtcctcg ccggtcagcg
     2281 agtgcggtgc cgggaaatag agcgccaggc cggcgtcgat gggctcgccg tcggcaccga
     2341 cgaattcgcg gagcgttgcg atacgcgggg cgggaatgcc gcccgtcagc gattcggcga
     2401 tcgcacggca cccaggtccc gacacccgca cgatcccgat gccgccgcgt ccggccggtg
     2461 tggcgatcgc ggcgatcgtg tccgaatcag gggacattca cctatttcgg caggtcacgg
     2521 gtagcaggtg acgaagctgc tggcggagaa attcgggtga atgtccccag tttcctgctc
     2581 accccggctt cttcgcctcg gccgccacca ggcgattgat gttccactgc tgcgcgatcg
     2641 acagcaccgt gttggtgatc cagtagagca cgaggcccga cgggaaccac gccatcatga
     2701 ccgtcatgac gagcggcatg aacgcgaaca ccttcgcctg catcgggtcc ggcggcgccg
     2761 ggttcagctt gaactggccg tacatcgccg cgcccatgat ggccggcagg atgaagtacg
     2821 gatccttcga cgacaggtcc tggatccaca gcatgaacgg cgcctggcgc at
//
LOCUS       DAOMFD010000245         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_18748_length_2829_cov_30.676640, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000245 DAOMFD010000000
VERSION     DAOMFD010000245.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18748_length_2829_cov_30.676640"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            66..548
                     /gene="folK"
                     /locus_tag="PL152_10425"
     CDS             66..548
                     /gene="folK"
                     /locus_tag="PL152_10425"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HQR49741.1"
                     /translation="MPAYVALGSNLDDPSAQVLRAFDALAGLPGSRLVAKSSLYRSPP
                     LGPVAQPDFVNAVAGLLTTLSPADLLAELKRLEERLGRAQPVVRWGPRRIDLDLLVHG
                     IHRITEPGLTVPHPGLAERAFVLGPLAEIAPDLDVPDMGRVRALAARLTSSALQRIAA
                     "
     gene            545..1234
                     /locus_tag="PL152_10430"
     CDS             545..1234
                     /locus_tag="PL152_10430"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010158266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HQR49742.1"
                     /translation="MSIAPRNPKDEPQVVHTPHRFVVVEGPIGVGKTSLARRLATSFG
                     SDLVLEQGEENPFLERFYRNPRAAAFQTQLYFLFQRARQMQDLRQQDMFQRVRVSDYL
                     LEKDRLFARLTLDDEEYGLYEQVYARLAIDAPVPDLVVYLQAPVDVLLERIGRRGIAY
                     EQGIERRYLERLADAYARFFLEYEAAPVLTVNASEIDPVGSERDYEALLAEVVRVRRG
                     RHYFNPMKSLL"
     gene            1384..2211
                     /gene="panB"
                     /locus_tag="PL152_10435"
     CDS             1384..2211
                     /gene="panB"
                     /locus_tag="PL152_10435"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HQR49743.1"
                     /translation="MYTQLQLRGSARPPVNVSTLRRMKDEGEKIASVTCYDASYAALV
                     DEADADVVLVGDSLGMVIQGYDTTVPVTLEQVIYHCRAVTRGLARPFLMADMPFMTYT
                     SREQALQNSVRLMQEGGAKMVKLEGGAGQVDIVEFLASHDIPVCAHLGLKPQSVHKTG
                     GFRVQGREEAAADQMRRDAKALQDAGADIVLLECIPSQLGKEITAELHVPVIGIGAGP
                     DTDGQILVLYDLLDITPGRKPRFVQNFMAGHDSPLEAVKAYVQAVKSRAYPAPEHCF"
     gene            2223..>2829
                     /gene="panC"
                     /locus_tag="PL152_10440"
     CDS             2223..>2829
                     /gene="panC"
                     /locus_tag="PL152_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HQR49744.1"
                     /translation="MLTVTRIAELREIVREWRLARESIAFVPTMGNLHAGHASLVGAA
                     HTHGRRVVASVFVNPLQFGPNEDYNAYPRTPADDSNLLSELGADVLFLPTVEEMYPKG
                     TTGSTIVDVPDIANILCGAFRPGHFQGVATVVVKLLNLVQPDVAIFGEKDYQQLTVIR
                     TSVEDLCLPVKIVGAPTVRAEDGLALSSRNRYLTPEERERAP"
BASE COUNT          427 a          988 c          969 g          445 t
ORIGIN      
        1 cgaccccgtc gccgtcgtcg gcgtcgtgcg gcgtgacgac cgtgccggcg aatgcgacgc
       61 gctggatgcc ggcctacgtc gcgctcggca gcaacctcga tgatccgtcg gcacaggtgc
      121 tgcgggcgtt cgatgcactc gccggactgc ctggctcgag gctggtcgcg aagtcgtcgc
      181 tgtatcgatc gccgccgctg ggcccggtcg cccagcccga tttcgtcaat gcggtggcgg
      241 gactcctgac cacgctctcg ccggcggacc tgctcgccga attgaagcgc ctcgaggagc
      301 gactcggccg cgcccagccg gtcgtgcgct gggggccgcg gcgcatcgac ctcgacctgc
      361 tggtgcacgg catccatcgc atcacggagc ccggcctcac ggtgccgcat cccggcctcg
      421 ccgagcgcgc gttcgtgctg ggtccgctcg cggagattgc gccggacctc gatgtcccgg
      481 acatggggcg cgtgcgtgcg ttggccgccc gactcacgtc ttcggctctg cagcggatcg
      541 cggcatgagc atcgcgcctc gcaacccgaa ggacgagccg caggtcgtcc acacgccgca
      601 ccggttcgtc gtcgtcgaag gcccgatcgg cgtcggcaag acgagcctcg cgcgccggct
      661 ggcgacgagt ttcggcagcg acctcgtgct cgagcagggc gaggagaatc cgttcctcga
      721 gcgcttctac cgcaatcccc gggccgccgc gttccagacg cagctctact tcctgttcca
      781 gcgcgcgcgc cagatgcagg acctgcggca gcaggacatg ttccagcggg tccgcgtgtc
      841 cgactacctg ctcgagaagg accgcctgtt cgcgcggctc acgctcgacg acgaggagta
      901 cgggctctac gagcaggtct atgcgcgact cgcgatcgac gcgccggtgc cggacctcgt
      961 cgtgtacctg caggcaccgg tggacgtgct gctcgaacgc atcggtcgcc gcggcatcgc
     1021 ctacgagcag ggcatcgagc gccggtatct cgagcggctc gccgatgcat acgcgcggtt
     1081 ctttctcgaa tacgaggccg cgccggtcct gaccgtgaac gcctcggaaa tcgacccggt
     1141 cggcagcgag cgggactacg aggcgctgct ggccgaggtc gtgcgagtac gccgcgggcg
     1201 gcactacttc aacccgatga agagcctcct gtagtaattt ggggtctgac cccgactgtg
     1261 gggtctgacc ccgattccct ggcgccgggc actcgaccgc cagcgtcggg aagtccggat
     1321 ccgccgctca ttgacggttt ccccggcact cggtcatcct tgccgtccct tcgaggccgt
     1381 cccatgtaca cacaactcca gctgcgcggt tcggcccgcc ccccggtcaa tgtctcgacg
     1441 ctgcgtcgca tgaaggacga gggcgagaag atcgccagcg tcacctgcta cgacgcgagc
     1501 tacgccgcgc tcgtcgacga ggcggacgcg gacgtcgtgc tggtcggcga ctcgctcggc
     1561 atggtgatcc aggggtacga cacgaccgtg ccggtgacgc tcgagcaggt gatctatcac
     1621 tgccgcgcgg tgacccgcgg cctggcgcgg ccgttcctca tggccgacat gccgttcatg
     1681 acctacacct cgcgtgagca ggctttgcag aactccgtgc ggctgatgca ggagggcggg
     1741 gccaagatgg tcaagctcga gggcggcgcc ggccaggtcg acatcgtgga attcctcgcc
     1801 agccacgaca tcccggtctg cgcgcacctt gggctcaagc cgcagtcggt gcacaagacc
     1861 ggcggcttcc gcgtgcaggg gcgcgaggag gcggccgccg accagatgcg tcgcgacgcg
     1921 aaggcgctgc aggacgccgg cgcggacatc gtgctgctcg agtgcatccc gtcgcagctc
     1981 ggcaaggaga tcacggccga gctgcacgtg ccggtgatcg gcatcggcgc cggaccggac
     2041 acggacgggc agatcctcgt gctctacgac ctgctcgaca tcacgccggg ccgcaagccc
     2101 cgcttcgtgc agaacttcat ggccggccac gacagcccgc tcgaggccgt caaggcctac
     2161 gtccaggccg tcaagtcgcg cgcctacccg gcgccggagc actgcttcta gcagtggctt
     2221 ccatgctgac cgtcactcgc atcgccgagt tgcgcgagat cgtgcgtgag tggcggctcg
     2281 cgcgcgagag catcgcgttc gtgccgacca tgggcaacct ccacgccggg cacgcgagcc
     2341 tggtcggtgc cgcgcacacg cacggccggc gcgtggtggc gagcgtgttc gtgaacccgc
     2401 tgcagttcgg gcccaacgag gactacaacg cgtacccgcg cacgccagcg gacgattcga
     2461 atttgctgtc ggagctgggc gcggacgtgc tgttcctgcc caccgtggag gagatgtatc
     2521 cgaagggcac gaccggcagc accatcgtcg acgtgccgga catcgccaac atcctgtgcg
     2581 gggcgttccg gcccgggcac ttccagggcg tggcgaccgt ggtggtgaag ctcctgaacc
     2641 tcgtgcagcc ggacgtcgcg atcttcggcg agaaggacta ccagcagctc acggtgatcc
     2701 gcacgtcggt cgaggacctg tgcctgccgg tcaagatcgt gggcgcgccg acggtgcgcg
     2761 cggaagacgg cctggccttg agctcgcgca atcgctacct cacgcccgag gagcgcgagc
     2821 gcgcgccgc
//
LOCUS       DAOMFD010000246         2824 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_18801_length_2824_cov_30.947996, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000246 DAOMFD010000000
VERSION     DAOMFD010000246.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2824)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2824
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18801_length_2824_cov_30.947996"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="PL152_10445"
     CDS             complement(<1..246)
                     /locus_tag="PL152_10445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR49745.1"
                     /translation="MSPASKALVAQAQSQRKKGDLPGATASLERALHIEPSNPLLWIE
                     MGRLRMDQRNYAQAEGMGRKALAMSVGDDRTQSLAWQL"
     gene            complement(524..>2824)
                     /gene="mrcB"
                     /locus_tag="PL152_10450"
     CDS             complement(524..>2824)
                     /gene="mrcB"
                     /locus_tag="PL152_10450"
                     /inference="COORDINATES: protein motif:HMM:TIGR02071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1B"
                     /protein_id="HQR49746.1"
                     /translation="KRLRRVVAVLAALAFVVAVVIVAVLDHRVTRQFEGRRWTLPARV
                     YAQPIELYVGQSLSAARAAEELDRLGYIEQARIERPGMYRRRGDRIDVYVRAFRFAEE
                     MQPARALTIGFAGDAIVSLREAGGADVPVFRLDPMLIGSIFPIHGEDRIVVSPAQVPP
                     LLPEALKAVEDRKFDTHHGVNPLAILRAFAVNVRAGQVEQGGSTLTQQLVKSYFLDSR
                     RTLGRKVEEAIMAVILESRFSKADIMNAYINEIYLGQDGQRAIHGFGLASQYYFGKPL
                     AELDLSQISLMVAIVRGPSYYDPRRHPERALARRNLVLQVLADRGVVTADAAARAAAK
                     PLGVGEAGRKGAIFFPAFLDLVRRQLRADYKEEDLTEAGLVVFTTLDPLLQEKAEDAL
                     ASELDRQDASSRRVSHGLEGVVVVTTPQTGEVVAIVGGRHASFDGFNRALDARRPIGS
                     LAKPLVYLTAFETGRYNPASIVHDQPVSLKLPNGQTWTPANFDKEVRGDMPAIRALTQ
                     SLNLPTVNLGMEVGLPKVARTFVQLGLAEAPAAYPSMLLGATNLSPYDVAQMYNTLAN
                     GGFRSPLRAVRSVVAETGKQLKAPELEVAEAAPADAVYMLDRMLIEVMNLGTGRPARR
                     SLPAGLVVAGKTGTSNDYRDSWFAGFSGAHLVVVWMGHDDNSQTGLTGTTGALAAWSK
                     LMSSITTTSFEPLMPESIDDRWIDYYSGLETSPSCDSAALSLPFRIGTALPADLACPP
                     GLADGAATQPGDFGTATGIPDTPLAP"
BASE COUNT          415 a          984 c         1006 g          419 t
ORIGIN      
        1 gagttgccac gccagggact gcgtccggtc gtcgcccacc gacattgcga gcgccttgcg
       61 ccccattccc tcggcctggg cgtagttccg ctggtccatc cgcaggcgcc ccatctcgat
      121 ccacagcagg ggattgctgg gctcgatgtg cagcgctcgc tccagcgacg ccgtggcacc
      181 cgggaggtcg cccttcttgc gctgggactg cgcctgggcg accagcgcct tgctggctgg
      241 gctcaaggtg gctgcgggcg ccttgggccg ctccttgggc agcggagtgg ggctcggcgc
      301 ggcgatgacg ggctcctggc cgggcgacgc gatgaccggt ggtgaactca gtaccggtcc
      361 ggacccgggt gacctggtcg cgctcccacg ggacgacgac ggggctggct cgctcttctg
      421 gaatgtatcc agggcgcagc ctgcggcggc caccgacagt ccaatcagcg cgacgatagt
      481 gcggagcatg gcggccgatt gtccggcccg cgaccgacgc agatcaaggc gccagcggcg
      541 tgtccggtat acccgtggcg gttccgaagt cccccggttg cgtcgcggcg ccgtccgcga
      601 gccccggcgg gcacgcgaga tcggccggca gcgcggtgcc gatccggaac ggcaggctca
      661 gggccgcgct gtcgcaagag ggggacgttt cgaggcccga gtagtaatcg atccagcggt
      721 cgtcgatcga ctcgggcatg agcggctcga acgaggtggt cgtgatcgac gacatcagct
      781 tcgaccatgc ggccagcgcg cccgtggtgc ccgtgagacc ggtctgcgag ttgtcgtcgt
      841 gtcccatcca gacgacgacg aggtgcgcgc cggaaaaacc ggcgaaccag ctgtcgcgat
      901 agtcgttgga cgtgccggtc ttgcccgcca ccacgaggcc cgccggcagc gaccggcgcg
      961 cggggcggcc ggtgccgagg ttcatcacct cgatcagcat ccggtcgagc atgtagacgg
     1021 cgtccgccgg ggccgcctcg gccacctcga gttccggcgc cttgagctgc ttgccggtct
     1081 ccgccacgac cgaacgcacg gcacgcaggg gcgagcggaa gccgccgttc gccagcgtgt
     1141 tgtacatctg ggcgacgtcg tacggactca ggttggtcgc gccgagcagc atggacgggt
     1201 acgccgcggg cgcctcggcg agaccgagct ggacgaatgt ccgcgcgacc ttgggcagcc
     1261 cgacctccat cccgaggttc accgtcggca ggttgaggga ctgggtgagc gcccggatcg
     1321 ccggcatgtc gccgcgcact tccttgtcga agttcgccgg cgtccaggtc tggccgttcg
     1381 gcagcttgag cgacacgggc tggtcgtgaa cgatcgacgc cgggttgtac cggccggtct
     1441 cgaaggccgt caggtacacc agcggcttcg cgagcgaacc gatcgggcgc ctggcgtcga
     1501 gtgcgcggtt gaagccgtcg aaggacgcat gccgcccgcc gacgatcgcc accacctcgc
     1561 ccgtctgcgg cgtcgtgacg accacgacgc cctcgagacc gtgcgacacc ctgcgcgacg
     1621 aggcgtcctg ccgatcgagt tccgaggcga gggcatcctc ggccttctcc tgcagcagcg
     1681 ggtcgagggt ggtgaagacg acgagcccgg cctccgtgag atcctcctcc ttgtagtccg
     1741 cgcgcagctg gcgacgcacg aggtcgagga aggccgggaa gaagatcgcg cccttgcggc
     1801 cggcctcgcc gacgccgagc ggcttcgcgg cggcccgggc cgccgcatcc gccgtgacga
     1861 cgccgcgatc cgcgagcacc tggagcacca ggttgcgccg tgcaagcgcg cgctccgggt
     1921 ggcggcgcgg gtcgtagtac gaaggcccgc gcacgatcgc gaccatgagg gagatctgcg
     1981 agaggtcgag ttccgccagc ggcttgccga agtagtactg gctcgcgagc ccgaagccgt
     2041 ggatcgcgcg ctggccgtcc tgcccgaggt agatctcgtt gatgtaggcg ttcatgatgt
     2101 ccgccttcga gaaccgcgac tcgaggatga cggccatgat cgcctcctcg accttccgcc
     2161 cgagcgtgcg ccggctgtcg aggaaatagc tcttcacgag ctgctgcgtg agcgtgctgc
     2221 cgccctgctc cacctgtccc gcccgcacgt tgacggcgaa cgcgcgcagg atcgcgagtg
     2281 gattgacgcc gtggtgcgtg tcgaacttgc ggtcctccac cgccttgagc gcttccggca
     2341 gcagtggcgg cacctgcgcc ggcgacacca cgatgcggtc ctcgccgtgg atcgggaaga
     2401 tgctgccgat cagcatcgga tcgaggcgga acaccgggac gtcggcgcca ccggcctcgc
     2461 gcagcgacac gatcgcgtcc ccggcgaacc cgatcgtgag cgcccgcgca ggctgcatct
     2521 cctccgcgaa gcggaaggcc cgcacgtaaa cgtcgatgcg atcgccgcgg cgccggtaca
     2581 tgcccggccg ctcgatccgc gcctgctcga tgtacccgag gcggtcgagt tcctcggcgg
     2641 cgcgcgccgc cgacagcgac tgcccgacgt agagctcgat cggctgggcg tacacgcgcg
     2701 ccggcagcgt ccagcgccgc ccctcgaact ggcgggtgac gcggtggtcg agcaccgcca
     2761 cgatcaccac cgcgacgacg aacgcgagcg ccgccagcac cgcgacgacg cgacgcagcc
     2821 gttt
//
LOCUS       DAOMFD010000247         2812 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_18964_length_2812_cov_31.841494, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000247 DAOMFD010000000
VERSION     DAOMFD010000247.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18964_length_2812_cov_31.841494"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1021
                     /locus_tag="PL152_10455"
     CDS             <1..1021
                     /locus_tag="PL152_10455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HQR49747.1"
                     /translation="ELNEFIVQHLRESILVVDAEYDVRLINETAARLLTGGPVASGTP
                     LSEVSPRLLYLLETWRRQSSDRRDSTGEVVSSDGGTVIRPHFVPLSETGPGPVLVFLE
                     DTSLVAERVQQSKLAALGRLSASIAHEIRNPVGAMSHAGQLLRESPAIGDDDRHLTDI
                     IEKNAARVSRIIDNVLQLSRRETTRQERIELRHWLQGFVQEFAATVQVPTSRFDLRGD
                     APSIEVHFDPMQLHQVLWNLCDNALKHAADARPELILLRAGRIASTGRPFLEIADRGT
                     GIGPANAERIFEPFFTGKTGGTGLGLFISRELCQTNGAVLAYEARSGGGSIFRIIFAD
                     PKRWE"
     gene            1120..1371
                     /locus_tag="PL152_10460"
     CDS             1120..1371
                     /locus_tag="PL152_10460"
                     /inference="COORDINATES: protein motif:HMM:NF013561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HQR49748.1"
                     /translation="MGMKTAVSLPDLLFQSAERLAARMGVSRSELYRRALEDLLARHD
                     ESQVTAQLNDVYADPADNAGLEPGLAELQFRSVATGRPR"
     gene            1368..1709
                     /locus_tag="PL152_10465"
     CDS             1368..1709
                     /locus_tag="PL152_10465"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HQR49749.1"
                     /translation="MNRGEIWWAPLPVPKGSGPGFRRPVLIVSADSFNRSRLATVIVA
                     TITTNERLAAAPGNVRIGGRRSGLSQPSVVNVSQLLTVDRKFLESRAGRLDASQLETV
                     DRGLRLALGLV"
     gene            1764..>2812
                     /locus_tag="PL152_10470"
     CDS             1764..>2812
                     /locus_tag="PL152_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HQR49750.1"
                     /translation="MRQPRALVVDDEPDIRDLLSITLGRMDIAVDTVGDRATAVKRLG
                     SERYDLVLTDMRLPDGDGLDLVGWIQDHRPGVPVAVITAHGNVEAAVRALKLGAFDFI
                     SKPLDLAALRKLIAATLRLGVDIEETGRRSAMRLHGLSKPMQQLRDLVARVARSQAPV
                     HISGDSGTGKELVARLIHESGPREGGPFVPVNCGAIPTELMESELFGHRKGSFTGAVA
                     DKEGLVRSAEGGTLFLDEVADLPLHMQVKLLRVIQEKTVRPVGDTREVPVDVRILSAT
                     HRRLDELVKAGKFREDLYYRINVIELHVPALRERLDDVPQLVDVLLDKVSRQIGVPCP
                     AISDDAMDKLLSYSYP"
BASE COUNT          460 a          975 c          948 g          429 t
ORIGIN      
        1 cgagctgaac gagttcatcg tgcagcacct gcgcgagagc atcctggtgg tggacgccga
       61 gtacgacgtg cgcctcatca acgagaccgc ggcgcgcctg ctgacgggcg gcccggtcgc
      121 aagcggcacg ccgctgtcgg aggtctcgcc gcgattgctc tacctgctcg agacgtggcg
      181 tcgccagtcc tccgaccgac gcgattccac cggcgaggtc gtgtcgtcgg acggcggcac
      241 cgtgatccgc ccgcacttcg tgccgctgtc ggaaaccggg ccggggccgg tgctggtgtt
      301 cctcgaggac accagcctcg tcgccgagcg cgtccagcag tcgaagctcg cggcgctcgg
      361 ccgcctgagc gccagcatcg cgcacgagat ccgcaacccc gtcggcgcga tgagccacgc
      421 cggacagctg ctgcgcgagt cacccgcgat cggcgacgac gaccgccacc tcaccgacat
      481 catcgagaag aacgcggcac gcgtgagccg catcatcgac aacgtgctgc agctgtcgcg
      541 gcgcgaaacg acgcggcagg agcgcatcga gctgcgccat tggctgcagg gcttcgtgca
      601 ggagttcgcg gcgacggtgc aggtgccgac ctcgcggttc gacctgcgcg gcgacgcgcc
      661 gtcgatcgag gtgcacttcg acccgatgca actgcaccag gtgctgtgga acctgtgcga
      721 caacgccctg aagcacgccg cggacgcccg gccggaactg atactgttga gggctggccg
      781 catcgcctcc accggccgcc cgttcctcga gatcgccgat cgcggcaccg gtatcggccc
      841 cgccaacgcc gagcgcatct tcgagccgtt cttcaccggc aagaccggag gaactggact
      901 ggggctgttc atctcgcgcg agctgtgcca gaccaacggg gccgtgctgg cctacgaggc
      961 acgatccggc ggtggcagca tcttccgcat catcttcgcc gacccgaaac gctgggagta
     1021 aggcgccacc gctgcggcgt cgcagcctga ttcgatgcga atgcggtcga ttaccgtcgg
     1081 gacggacggg cgccacggcg gtataaagaa agttatacta tgggcatgaa gaccgccgtg
     1141 tcactgccgg atctcctgtt ccagtccgcc gaacgactcg cggcgcggat gggcgtgtcc
     1201 cgtagcgaac tgtatcggcg ggcactcgaa gacctgcttg cgcgccacga cgagagccag
     1261 gtcaccgcgc agctcaatga tgtgtatgcc gatccggcgg acaatgccgg actggagccc
     1321 ggccttgcgg aactgcagtt caggtcggtt gcgacaggaa ggccccggtg aaccgcggcg
     1381 agatctggtg ggcaccgctg ccggtaccga aaggttcggg acccggcttc aggaggccgg
     1441 tgctcatcgt ttccgcggac agcttcaatc gcagccgcct ggcgacggtc atcgtcgcga
     1501 cgatcaccac gaacgagcgc ctggccgcgg cgccgggtaa cgttcgcatc ggcggcaggc
     1561 gcagcggtct ttcgcagccc tcggttgtca atgtgtctca gctcctgacc gttgaccgga
     1621 aattccttga atcacgcgcc ggcaggctgg atgcctcgca gctcgaaacg gtcgaccgcg
     1681 gactcaggct ggcgctcggc ctggtgtgat tcggtatagt ctcgcccttg acgcaaggac
     1741 acccgacccg gacggaccga gcgatgcgac agccccgcgc actcgtcgtc gacgacgagc
     1801 ccgacatccg ggacctgctg tcgatcacgc tcggccgcat ggacatcgcc gtcgacaccg
     1861 tcggcgaccg cgcgacggcg gtgaagcgtc tcggctccga gcgctacgac ctcgtgctca
     1921 ccgacatgcg cctgccggac ggcgacggac tcgacctcgt cggctggatc caggaccacc
     1981 gcccgggcgt gccggtcgcg gtgatcacgg cgcacggcaa tgtcgaggcc gcggtccgcg
     2041 cgctcaagct cggcgcattc gatttcatct ccaagccgct cgacctcgcc gcgctgcgca
     2101 agctgatcgc ggcgacgctc cggctcggtg tcgacatcga ggagaccggc cggcgctcgg
     2161 cgatgcgcct gcacggcttg tcgaagccga tgcagcagtt gcgcgacctg gtcgcccgcg
     2221 tcgcacgctc gcaggcgccg gtgcacattt ccggcgactc cggcaccggc aaggaactgg
     2281 tggcgcgcct catccacgag tcgggcccgc gcgaaggcgg tcccttcgtg cccgtgaact
     2341 gcggtgcgat ccccaccgag ctgatggaaa gcgagctctt cggccaccgc aagggctcgt
     2401 tcacgggcgc ggtcgccgac aaggaaggcc tggtgcgcag cgccgagggc ggcacgctgt
     2461 tcctcgacga ggtcgccgac ctgccgctgc acatgcaggt gaagctgctg cgcgtgatcc
     2521 aggagaagac ggtgcgcccg gtcggcgaca cgcgcgaagt gccggtggac gtgcggatcc
     2581 tgtcggccac ccatcgccgc ctcgacgagc tggtcaaggc cggcaagttc cgcgaggacc
     2641 tctactaccg catcaacgtc atcgagctgc acgtgccggc gctgcgcgag cggctcgacg
     2701 acgtgccgca gctcgtcgac gtgctgctcg acaaggtgtc gaggcagatc ggcgtgccgt
     2761 gcccggcgat cagcgacgac gccatggaca agctcctgtc ctactcgtac cc
//
LOCUS       DAOMFD010000248         2744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_19813_length_2744_cov_36.425809, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000248 DAOMFD010000000
VERSION     DAOMFD010000248.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2744
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19813_length_2744_cov_36.425809"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2114)
                     /gene="ppsA"
                     /locus_tag="PL152_10475"
     CDS             complement(<1..2114)
                     /gene="ppsA"
                     /locus_tag="PL152_10475"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008986 - pyruvate, water dikinase
                     activity [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HQR49751.1"
                     /translation="MSRYTIPFAELGRHDVATVGGKNSSLGEMIANLAALGVSVPGGY
                     ATTADAYREFLAHGGLAGRIKAELDALDVDDVAQLAESGARIRDWVLSTPFPAALERE
                     ITEGYARMCDGRGDLSVAVRSSATAEDLPEASFAGQQETLLNVRGRANVVKAVHEVFA
                     SLYNDRAIAYRVHQGFDHSQVALSAGIQHMVRSDLGSSGVMFTLDTDSGFRDVVFITA
                     SWGLGETVVQGAVNPDEFYVYKPALRAGHEPVLRRTLGGKAIKMTYAQAGDGGQRVVT
                     VDVPEADRLRFCLDDADLRELARQALVIEDHYGCPMDIEWGKDGETGRIYILQARPET
                     VQSRAGRTLQRYTLESRGKVLASGRSIGHRIGAGPARVIRDVREMARVESGDVLVADM
                     TDPDWEPVMKRASAIVTNRGGRTCHAAIIARELGIPAVVGCGNATTSIAEGQPVTVSC
                     AEGDTGYVYDGMLRFDQSQVELDAMPQLPVKIMMNVGNPDRAFDFAAIPHRGVGLARL
                     EFIINRMIGVHPKALLGFDGLDPELKDTIRRQMAGYSDPVGFYIAKLCEGIGTIAAAF
                     APEPVIVRLSDFKSNEYANLIGGRQYEPVEENPMLGFRGAARYVDEGFRPCFELECRA
                     LKRVRSEMGLKNVWVMVPFVRTLREAEQVVELLAANGLKRGEDGLKVIMMCEVPTNAL
                     LADRYLEFFDGMSIGSNDMTQLT"
     gene            2189..>2744
                     /locus_tag="PL152_10480"
     CDS             2189..>2744
                     /locus_tag="PL152_10480"
                     /inference="COORDINATES: protein motif:HMM:NF015575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HQR49752.1"
                     /translation="MNRRTVFFVSDQTGVTAETMGHSLLTQFEGLEFRQVTLPFIASV
                     DKAEEAVRKIDATGAADGIRPIVFSTLVKAELRSVVKRSNGLFLDFFDAFMSPLEGEL
                     EVKSSERAGRAHGIADQVVYGARIDATNFALSADDGGVVADYDRADVILIGVSRSGKT
                     PTCIYMAMQYGVFAANYPLTEDDFE"
BASE COUNT          449 a          919 c          924 g          452 t
ORIGIN      
        1 gtgagttgcg tcatgtcgtt cgagccgatg gacatgccgt cgaagaactc gaggtaacgg
       61 tccgcaagca gtgcgttggt cggcacctcg cacatcatga tgaccttgag cccgtcctcg
      121 ccgcgcttga ggccgttggc cgccagcagt tcgacgacct gctcggcttc acgcagcgtg
      181 cgcacgaagg gcaccatcac ccagacgttc ttcagtccca tctccgagcg cacgcgcttc
      241 aacgcgcggc attcgagctc gaagcacggc cggaagccct cgtcgacata acgcgcggca
      301 ccgcgaaacc cgagcatcgg gttctcctcg acgggctcgt actgccggcc gccgatcagg
      361 ttcgcgtact cgttggactt gaagtccgac aggcgcacga tcaccggctc cggcgcgaat
      421 gccgccgcga tcgtgccgat gccctcgcac agcttcgcaa tgtagaaccc gaccgggtcg
      481 gagtagccgg ccatctggcg ccggatcgtg tccttgagtt ccgggtcgag gccgtcgaag
      541 ccgagcagcg ccttcgggtg gacgccgatc atgcggttga tgatgaactc gagccgcgcg
      601 agcccgacgc cgcggtgcgg aatcgcggcg aagtcgaatg cgcggtcggg attgccgacg
      661 ttcatcatga tcttgacggg gagctgcggc atcgcgtcga gctcgacctg cgactggtcg
      721 aagcgcagca tgccgtcgta cacgtacccg gtgtcgccct cggcgcacga caccgtgacc
      781 ggctggccct ccgcgatcga ggtggtcgcg ttgccgcagc cgacgacggc cgggatgccg
      841 agttcgcgcg cgatgatcgc ggcgtggcag gtgcgcccgc cgcgattggt gacgatggcc
      901 gaggcgcgtt tcatgacggg ttcccagtcg ggatcggtca tgtcggcgac cagcacgtcg
      961 ccgctctcga cgcgcgccat ttcgcgcacg tcgcggatca cgcgggcggg gcccgcgccg
     1021 atgcggtgac cgatgctgcg gccgctcgcc agcaccttgc cgcgagactc gagcgtatag
     1081 cgctgcagcg tccgtccagc gcggctctgc accgtctccg gccgcgcctg caggatgtag
     1141 atccggccgg tctcgccgtc ctttccccac tcgatgtcca tcgggcagcc gtaatggtcc
     1201 tcgatgacca gcgcctgccg tgccagttcg cgcaggtcgg cgtcgtcgag gcagaagcgc
     1261 aggcggtccg cctccggcac gtcgaccgtc accacgcgct ggccaccgtc accggcctgt
     1321 gcgtaggtca tcttgatcgc cttgccaccc agcgtccggc gcaggaccgg ctcgtggccc
     1381 gcgcgcaaag ctggcttgta gacgtagaac tcgtccggat tcaccgcacc ctgcacgacg
     1441 gtctcgccca ggccccacga cgccgtgatg aacacgacgt cgcggaatcc ggagtcggtg
     1501 tcgagcgtga acatcacgcc gctcgagccg aggtcgctgc gcaccatgtg ctggatgccg
     1561 gcggacaagg cgacctggga gtggtcgaat ccctggtgca cccggtaggc gatcgcgcgg
     1621 tcgttgtaga gcgacgcgaa cacctcgtgc acggccttca cgacgttcgc gcgaccgcgc
     1681 acgttgagca gcgtttcctg ctggccggcg aacgaggcct cgggcaggtc ttccgccgtc
     1741 gctgacgatc gcacggcgac cgacaggtcg ccgcggccgt cgcacatccg cgcataacct
     1801 tcggtgatct cacgctccag cgcggcgggg aacggcgtcg acagcaccca gtcgcggatg
     1861 cgggcgccgc tttcggcaag ctgcgcgacg tcgtcgacgt cgagcgcgtc gagttccgcc
     1921 ttgatccggc cggcaaggcc cccgtgggcg aggaattcgc gataggcgtc cgccgtcgtg
     1981 gcgtagccgc ccgggaccga gacacccagc gcggcgaggt tggcgatcat ctcgccgagc
     2041 gaggaattct tgccgccgac ggtcgcgacg tcgtgccggc ccagctctgc aaacggaatc
     2101 gtgtagcggc tcaagggtcg cctcttgcta ctggcggtgg acaggtgaaa gactgcgctg
     2161 cgatggcgcc aaggagtgca acgcgcgcat gaaccggaga accgtgtttt tcgtctcgga
     2221 ccagacaggc gtgacggccg aaacgatggg acacagtctg ctcacgcaat tcgaaggcct
     2281 cgaattccgg caggtgactc tgccatttat agccagcgtt gacaaggcag aggaagccgt
     2341 aaggaagatc gacgcgacgg gtgccgcgga cggcatccgg ccgatcgtgt tcagcacgct
     2401 cgtcaaggcg gaactgcgct ccgtcgtcaa gcgcagcaac gggctgttcc tggacttctt
     2461 cgacgcgttc atgtcgccgc tcgaggggga actcgaggtc aagtcgtcgg agcgggctgg
     2521 ccgcgcgcac ggcatcgcgg accaggtcgt ctacggcgcc cgcatcgacg cgaccaactt
     2581 cgcgctgtcg gcggacgacg ggggcgtggt ggccgactac gatcgcgccg acgtgatact
     2641 catcggcgta tcacgttcgg gcaagacgcc gacgtgcatc tacatggcga tgcagtacgg
     2701 cgtgttcgcc gcgaactatc cgctcaccga ggacgacttc gaga
//
LOCUS       DAOMFD010000249         2742 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_19833_length_2742_cov_35.191291, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000249 DAOMFD010000000
VERSION     DAOMFD010000249.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2742
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19833_length_2742_cov_35.191291"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PL152_10485"
     CDS             <1..362
                     /locus_tag="PL152_10485"
                     /inference="COORDINATES: protein motif:HMM:NF022161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2505 domain-containing protein"
                     /protein_id="HQR49753.1"
                     /translation="IKRGGRAPNVSLEIFRREPANPPAAIRRLVPGVNDVSHTEKWAA
                     DGDGYSADIVVEIKGVPVKITGTKSLQPAKGGCVVEWKFDVTSGIPLLGGVIASFAGS
                     EIEQKLKKESGILSAMA"
     gene            complement(363..1148)
                     /locus_tag="PL152_10490"
     CDS             complement(363..1148)
                     /locus_tag="PL152_10490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49754.1"
                     /translation="MIKWRLMVTTLPYVAAVLVVKLVLQFQVGFQGLVEFGDVGMVFT
                     AGVFLLGFLLAGTMADYKEAEKIPAELASTLETIEEIFVLSATNHPRLDLASLRRSLL
                     GLVDTIKDWLLKRRPVADVYAAMTGFNGQIEELERHGAGPYASRAVPQMLAVRRSVSR
                     IDVIARTGFLPPAYALLEVLIAMILALVVIARFKNIVAECLLVPFIALINIYMLRLIR
                     DIDDPFDYEADGRQHGSGEVPLFPLDEYRERLAARIDGPRPGR"
     gene            1309..>2742
                     /locus_tag="PL152_10495"
     CDS             1309..>2742
                     /locus_tag="PL152_10495"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR49755.1"
                     /translation="MPSMSATDQGFLLFETAERPMNIGALLVLAPPTGTRGRFADQVV
                     AQMLRCRVGAPFSYRVKPGPVQPLLAFEDDPDMDPRKQVRRHALRRGSNLQDLFEKVC
                     ELHAPLLPHDQPLWQAHVFTGLPDGRVALYFKTHHALIDGMGFLRVLRGVVSTSARGS
                     KPHSIWEGMRAAPPAEVRGPPTSESASPALADKLLGAVTGLANLFFRQGQHELGLDRG
                     LLLPFVHVPPALKAPPSQGRVLAHCRLPLALARELARRGDAKINDVMLAVLDGALHRY
                     LHDRGTPSGSPLVVDIPIALQEDGGTGNRIVGLQVPMGLPAATPRERLRQIVQETRGM
                     KQAVRALSAASLETYSIASHVAASLIESLGLGALPMLANLVVSNPAGFEHRVYFNGAD
                     VELALPVSVVMPHQALNVTVTTYMDELHITFIAVREVMPDLAKLATYTVDAVDQLRRS
                     LARGSRAKAGPRAAARTRGRRAATARTR"
BASE COUNT          439 a          978 c          929 g          396 t
ORIGIN      
        1 ggatcaagcg cggcgggcgt gcgccgaacg tgagcctcga gatcttccgc cgcgagccgg
       61 cgaatccgcc cgccgcgatc cgcaggctgg tgccgggcgt gaacgacgtg tcgcacaccg
      121 agaaatgggc cgcggacggc gacggctaca gcgccgacat cgtcgtggag atcaagggcg
      181 tgcccgtgaa gatcaccggc accaagtccc ttcagccagc caagggcggc tgcgtggtcg
      241 agtggaagtt cgacgtgacg agcggcattc cgctcctcgg cggagtcatc gcgtcgttcg
      301 cggggtccga gatcgagcag aagctgaaga aggaatccgg gatcctctcg gcgatggcct
      361 gactagcggc ccggccgggg cccgtcgatg cgcgcggcca gccgctcgcg gtactcgtcc
      421 agcgggaaca gcggcacctc gccggacccg tgctggcgcc cgtccgcctc gtagtcgaac
      481 gggtcgtcga tgtcgcggat cagccgcagc atgtagatgt tgatgagcgc gatgaacggc
      541 actagcaggc actcggccac gatgttcttg aagcgcgcga tcacgaccag tgcgaggatc
      601 atcgcgatca gcacctcgag cagcgcgtac gccggcggca ggaaaccggt ccgtgcaatc
      661 acgtcgatgc gggacacact gcggcgcacg gccagcatct gcggcacggc ccgcgacgca
      721 tacggcccgg ccccgtgccg ttccagttcc tcgatctggc cgttgaaccc ggtcattgcg
      781 gcatagacgt cggccaccgg ccgccgcttc agcagccagt ccttgatcgt gtcgaccagc
      841 ccgagcaggc tgcggcgcag cgacgcgaga tcgagccgcg ggtggttggt ggccgagagg
      901 acgaagatct cctcgatcgt ctcgagcgtc gacgccagct ccgccgggat cttctccgcc
      961 tccttgtagt ccgccatggt gccggccagc aggaagccga gcaggaagac gccggcggtg
     1021 aacaccatgc cgacgtcccc gaattccacg aggccctgga acccgacctg gaactgcagc
     1081 acgagcttca cgacgaggac cgccgccacg tagggcagcg tcgtcaccat cagtcgccac
     1141 ttgatcactg ggtctcgtcg cctcccgcgc cggcgtccgg tgccgcagcc gcatgatggc
     1201 tggcgcgagc cgcggtgcac aagacgagtc gcgttcaacg tgaattgctg cagcgcatct
     1261 agccgaatgc gaagcgcggc cggatccgcg ccggggtagc attggctcat gccgagcatg
     1321 tccgccaccg accagggatt cctgctgttc gaaacggccg agcggccgat gaacatcggg
     1381 gccctgctcg tgctcgcgcc accgacgggc acccgcggca gatttgcgga ccaggtcgtg
     1441 gcgcagatgc tccggtgccg cgtcggcgcg cccttcagct accgggtcaa gccgggcccc
     1501 gtccagccgc tgctcgcgtt cgaggacgac ccggacatgg acccgcgcaa gcaggtgcgc
     1561 cgccatgccc tgcgccgggg cagcaacctg caggacctgt tcgagaaggt gtgcgaactg
     1621 cacgcgccgc tgctgccgca cgaccagccg ctgtggcagg cacacgtgtt cacgggcctg
     1681 cccgacggcc gcgtggcgct ctatttcaag acgcaccacg cgctcatcga cggcatggga
     1741 ttcctgcgcg tcctgcgcgg cgtcgtgtcg acgtcggcgc gcggcagcaa gccgcattca
     1801 atctgggagg ggatgcgagc cgcgccgcca gcggaggtcc gcggaccacc gacgagtgaa
     1861 agcgcatcgc ccgcgctcgc ggacaagctc ctcggcgcgg tgaccggcct ggcgaacctg
     1921 ttcttccgcc agggccagca cgaactcggc ctcgaccgcg gcctgctgct gccgttcgtc
     1981 cacgtgccgc cggccctcaa ggctccgccg agccagggtc gcgtgcttgc gcactgccgg
     2041 ctgccactgg ccctggcccg cgagctcgcg cgtcgcggcg acgcaaagat caacgacgtg
     2101 atgctcgccg tcctcgacgg tgcgctccac cgatacctgc acgatcgcgg cacgccgtcc
     2161 ggcagtccac tcgtcgtcga catcccgatc gcgctccagg aggacggcgg cacgggcaac
     2221 cgcatcgtgg gcctgcaggt gcccatgggc ctccccgcgg ccactcctcg cgaacggttg
     2281 cggcagatcg tgcaggaaac ccgcggcatg aaacaggcgg ttcgcgcgct gtcggccgcc
     2341 agcctcgaga cctactcgat cgcgtcccac gtggccgcga gcctcatcga gtcgctggga
     2401 ctcggcgcgc tgccgatgct ggcgaacctc gtcgtatcga acccggccgg gttcgagcat
     2461 cgcgtgtact tcaacggcgc ggacgtcgag ctcgcgctgc cggtgtcggt cgtgatgccg
     2521 caccaggcgc tcaacgtcac ggtcacgacc tacatggacg agctgcacat caccttcatc
     2581 gccgtgcggg aggtgatgcc ggatctcgcg aagctcgcga cctacacggt cgacgcggtg
     2641 gaccagctcc gccgctcgct ggcacgtggc tcccgtgcaa aggccggacc ccgggcggcg
     2701 gctcgcacca ggggccgccg cgccgcgacc gcgcgcacgc gc
//
LOCUS       DAOMFD010000250         2732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_19982_length_2732_cov_30.071722, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000250 DAOMFD010000000
VERSION     DAOMFD010000250.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19982_length_2732_cov_30.071722"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1104
                     /gene="dsbD"
                     /locus_tag="PL152_10500"
     CDS             <1..1104
                     /gene="dsbD"
                     /locus_tag="PL152_10500"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF001419.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047134 - protein-disulfide reductase
                     (NAD(P)) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HQR49756.1"
                     /translation="FTYTVAGAAFAAAGRQAQAFFQQPWIIVAFAGLFVLLALGMFGV
                     YNLQVPAAFQSRVADLSNRQKQGTLAGTAVMGALSSLIVTACVAPPLVAALTMIGQSG
                     NVFRGGAALFALSLGMGAPLLVVGASAGQLLPKAGAWMDGVKIAFGVMMLGVAIWMLS
                     RILPGALTLALWAGLAFVSGYCLLTLGATDGRTGWLAVRRGLGWLAILYGIAMLVGAL
                     AGRSDPLRPLEGLARSSAPAASGTRLALTRVKSVADFERELAAATAAGRPLMLDFYAD
                     WCVSCKEMEHQTFTDAGVQAVLASAVLLQADVTANDDEDQALLARFGILGPPTIVFFG
                     ADGAERKDFRVVGFQPAAEFRAHVAQAFGRNGA"
     gene            1101..1730
                     /locus_tag="PL152_10505"
     CDS             1101..1730
                     /locus_tag="PL152_10505"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR49757.1"
                     /translation="MKRVAYVALSVGAAGTGAAGYLAYQQYARNAAPPSPPAAPPMQL
                     AQRVPDFKLADREGKMRSLADWQGKSLIVNFWATWCAPCRREIPLLGRLQQHYGPDGF
                     QVIGIAADYRDKVVAYANDAHIEYPLLIGEQEALDAATAFGIQVVGFPFTVFSDRQGR
                     VVVCHVGELTQPIAEAILGIVERVNRGTLTLEDARGEIQTSLARIKGAS"
     gene            1872..2330
                     /gene="aroQ"
                     /locus_tag="PL152_10510"
     CDS             1872..2330
                     /gene="aroQ"
                     /locus_tag="PL152_10510"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HQR49758.1"
                     /translation="MAHILLLNGPNLNLLGAREPGIYGKVTLGQVHERMTKLAAEAGH
                     HLTAFQSNSESELIARIHDAPGSHVAFILFNPAGFTHTSVALRDALLAVKIPFIEVHL
                     SNVFAREAFRHHSYFSDIALGCITGLGAGGYELALRAASEYLAARSRGQA"
     gene            2357..>2732
                     /gene="accB"
                     /locus_tag="PL152_10515"
     CDS             2357..>2732
                     /gene="accB"
                     /locus_tag="PL152_10515"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HQR49759.1"
                     /translation="MDIRKVKKLIELLEESGISEIEIKEGEEAVRISRHPTGVVHAAH
                     APIAYAPHPAPAAAPGATPAAAAIATKPAVAPDQAVTAPMVGTFYASPAPGAKPFVEL
                     GSEVKPGDVLCIIEAMKMMNQIE"
BASE COUNT          404 a          975 c          915 g          438 t
ORIGIN      
        1 ttcacctaca cggtcgccgg cgcggccttc gcggctgccg ggcgccaggc gcaggcattc
       61 ttccagcagc cctggatcat cgtcgcgttc gccggcctgt tcgtgctgct ggcactcggc
      121 atgttcggcg tctacaacct gcaggtgccg gcggcgttcc agtcgcgcgt cgccgacctg
      181 agcaaccgcc agaagcaggg cacgctcgcg ggcaccgccg tcatgggcgc gctgtcgtcg
      241 ctcatcgtca cggcgtgcgt ggcacccccg ctggtcgcgg cgctcacgat gatcggccag
      301 agcgggaacg tcttccgcgg cggcgcggcg ctgttcgcgc tgagcctcgg catgggcgcg
      361 cctctcctcg tcgtcggcgc ctcggcgggg cagttgctgc ccaaagccgg cgcatggatg
      421 gacggcgtca agatcgcgtt cggcgtaatg atgctgggcg tcgcgatctg gatgctctcg
      481 cgcatcctgc ccggcgcgct cacgctcgcg ctgtgggcgg gtctcgcgtt cgtttccggc
      541 tactgcctgc tgaccctggg cgccacggac gggcggacag gctggctcgc ggtgcgccgc
      601 ggcctcggtt ggctcgcgat cttgtacggc atcgcgatgc tggtcggggc gctggccggt
      661 cgcagtgatc cgctccggcc actcgagggg ctggcccgtt cttccgcacc cgcggccagc
      721 gggaccaggc tggcgttgac gcgcgtgaaa tcggtcgccg acttcgaacg cgaactcgcc
      781 gcggcgacgg ccgcaggccg cccgctgatg ctcgacttct atgcggactg gtgcgtgtcc
      841 tgcaaggaga tggagcacca gaccttcacc gatgcgggcg tccaggccgt gctggcgagc
      901 gccgtgctgc tccaggcgga cgtcacggcc aatgacgatg aggaccaggc cctgctcgcg
      961 cgcttcggga tcctcggccc gccgaccatc gtgttcttcg gggccgacgg agccgagcgc
     1021 aaggacttcc gcgtcgtggg cttccagccg gcggccgagt tccgggcgca cgtcgcgcag
     1081 gcattcggta ggaacggcgc gtgaagcgcg tcgcatacgt cgcgctttcc gtcggcgcgg
     1141 ccggcactgg agcggccggg tatctcgcgt accagcagta cgcgcgcaat gctgcgccgc
     1201 cctcgccgcc cgcagcgccg ccgatgcagc tcgcccagcg cgttcccgac ttcaagctcg
     1261 ccgaccgcga gggcaagatg cggtccctgg ccgactggca gggcaagtcg ctgatcgtga
     1321 acttctgggc gacgtggtgc gcgccctgcc ggcgcgagat cccgttgctc ggccggctgc
     1381 agcagcacta tgggcccgac ggcttccagg tgatcggcat cgccgcggac taccgcgaca
     1441 aggtcgtcgc ctacgcgaac gacgcgcaca tcgagtaccc gctgctcatc ggcgagcagg
     1501 aggcgctcga tgccgccacc gcgttcggca tccaggtcgt cggctttcca ttcacggtct
     1561 tctccgaccg gcagggccgg gtcgtcgtct gccacgtggg agagttgacc cagcccatcg
     1621 cagaggccat cctcggcatc gtcgaacggg tgaaccgggg caccttgacc ctggaggacg
     1681 cccgagggga gatccagacg tcgctcgctc gcatcaaggg cgcctcatag gcctgcaccc
     1741 ggctggccgg gaggttggcc ggaccctggc cgccccgcca ctgccccacc ccccggatcg
     1801 aggggtatag tatcaatcgt gcagcgatcg gcgttaacct tgccgccttc tccgggctat
     1861 tcaccccagt catggcacat atcctgctgc tgaacggtcc gaacctgaac ctcctcggcg
     1921 cccgggaacc ggggatctac ggcaaggtga cccttggcca ggtccacgag cgcatgacca
     1981 agctcgccgc cgaggccggg caccacctca cggcattcca gagcaactcg gagtcggaac
     2041 tgatcgcccg catccacgac gcaccgggca gccacgtcgc cttcattctg ttcaatcccg
     2101 cgggcttcac gcacaccagc gtggcgctgc gcgacgcact gctcgcggtg aagatcccgt
     2161 tcatcgaggt gcatctttcg aacgtgttcg cccgggaggc cttccggcat cactcctact
     2221 tctccgacat cgccctcggc tgcatcaccg gcctgggcgc tggcggctac gaactcgccc
     2281 tgcgcgcggc gtccgagtac ctcgccgccc ggtcgcgcgg gcaagcctga caaacgacgg
     2341 atccagggag tcccgaatgg acatccgcaa ggtcaagaag ctcatcgaat tgctcgagga
     2401 atcgggaatc tcggaaatcg agatcaagga aggcgaggaa gcggtgcgca tcagccggca
     2461 tccgacgggc gtggtgcacg cagcgcacgc accgatcgcc tatgcgccgc accccgcccc
     2521 cgccgctgcg cccggcgcaa caccggccgc cgctgcgatt gcgaccaagc cagcggtggc
     2581 gcccgaccag gccgtgaccg cgcccatggt cggcaccttc tatgcgtcgc ccgcgccggg
     2641 cgccaagccg ttcgtggaac tcggcagcga ggtcaagccg ggcgacgtgc tgtgcatcat
     2701 cgaggccatg aagatgatga accagatcga gt
//
LOCUS       DAOMFD010000251         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_20401_length_2701_cov_38.628874, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000251 DAOMFD010000000
VERSION     DAOMFD010000251.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20401_length_2701_cov_38.628874"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="PL152_10520"
     CDS             complement(<1..443)
                     /locus_tag="PL152_10520"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HQR49760.1"
                     /translation="MRKILVFQHVASEPLGHLDRLLRTSGFRIRYVNFGREPHAEPDV
                     RRYDGLVVLGGPMNVDQADHLPHLRTEMAAIRACVEADKPMLGICLGAQLLAAAMGGI
                     VHANHVPEIGWYRLHTLQSAHEDPLFRHFERRPHYVFQWHAYTFAP"
     gene            552..1445
                     /locus_tag="PL152_10525"
     CDS             552..1445
                     /locus_tag="PL152_10525"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HQR49761.1"
                     /translation="MTGTYQPKRSPVHEQLVVRGLRHHVTRWPGAGETIVLLHGWADT
                     GDTFQFLVDAMSDQYSFVAPDWRGFGRTEWPTEGYWFPDYLGDLDAMLDAWSPDAAVT
                     MVGHSMGGNVATLYAGIRPERVRRLVCIEGFGLPRTQPDQAPGRYRQWLRELRETQAF
                     ASFPSLDAFAHLLMRRNPRLTPERARFIAQTWTRPAADGAVQVLGDPAHKRTNAVLYR
                     REEAEACWREIVAPVLYVMAAQSEYLPRLGADGRPEHMARLIRRLEPCVIEDASHMVH
                     HERPEALAHAIESFLAATPSL"
     gene            1470..>2701
                     /locus_tag="PL152_10530"
     CDS             1470..>2701
                     /locus_tag="PL152_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49762.1"
                     /translation="MSDGANDPRGWFAELKRRKVFRVAAVYLVAAWLVVQVAGTTFEP
                     MGLPAWTLKLVITLVVLGFPVACALAWAFDVTPRGIERTPVAAPPTGLALAHGSGSLA
                     PSHAAPAGDTTPTATPVTAPIGGPAASESADSVAILPFVDMSPERDQEYFCDGIAEEI
                     INSLCCIRDLRIASRTSSFQFKGRAADVREIGRTLGVGAVLEGSVRKAGSRVRITAQL
                     VSSTDGYHLWSESYDRELADVFAIQTEIAQKLVAALRVTLSREERELIQRRGTSNAEA
                     YDLYLRGQAHLRDGTDVAMGPASDCFRQAIRRDERFAQAHAGLANALALKGLWRIGMT
                     PADFEEAFAAGRRALELEPRMPEAYLAQAMLLSMQGRAAEADRSFEESIRLNPASFDA
                     HYMYGRHCFQSGAFDRAVP"
BASE COUNT          397 a          923 c          982 g          399 t
ORIGIN      
        1 ggcgcgaagg tgtaggcgtg ccactggaag acgtagtgcg gccggcgctc gaagtgacgg
       61 aacagcgggt cctcgtgcgc ggactgcagc gtgtgcaggc ggtaccagcc gatctccggg
      121 acgtggtttg cgtgcacgat gccacccatc gcggcggcca gcagctgcgc accgaggcag
      181 attccgagca tcggcttgtc cgcctcgacg caggcgcgga tcgctgccat ctccgtgcgc
      241 aggtgcggca ggtggtccgc ctggtcgacg ttcatcggtc cgcccagaac caccaggccg
      301 tcgtagcggc gcacgtcggg ctcggcgtgc ggctcgcgcc cgaaattcac gtagcggatg
      361 cggaacccgg atgtgcgcag cagtcgatcg aggtggccga ggggctcgga cgcgacgtgc
      421 tggaagacga ggatcttgcg catgggtcgg ggcggtacgc ggcgcgcaac tatatcggca
      481 gtgccggttc gcgcgcgagc ggacgcggcg ggtcgggtat gctcgcggcc tgtccgggcc
      541 aacgaggcga catgaccggg acctaccagc cgaaacgatc gccggtccac gagcagctcg
      601 tggtccgcgg gctgcgccac cacgtcacgc gctggcccgg cgccggcgag accatcgtcc
      661 tgctgcacgg ctgggccgat acgggcgaca cgttccagtt cctcgtcgac gcgatgtccg
      721 accagtactc gttcgtggcg cccgactggc gcggcttcgg tcgcacggag tggcccaccg
      781 agggctactg gttcccggat tacctcggcg acctcgacgc gatgctcgac gcgtggtcgc
      841 ccgacgcggc ggtgacgatg gtgggccaca gcatgggcgg caacgtcgcg acgctgtatg
      901 cggggatccg cccggagcgc gtccgacgac tcgtctgcat cgaggggttc ggcctgcccc
      961 ggacccagcc ggaccaggcg ccgggtcgct accggcagtg gctgcgcgag ctgcgcgaga
     1021 cgcaggcgtt cgcgagcttt ccgtcgctcg atgcgttcgc ccacctgctc atgcgccgca
     1081 atccgcggct gacgccggaa cgggcgcgat tcatcgcgca gacgtggacg agacccgcgg
     1141 cggatggagc ggtgcaggtt ctcggcgatc cggcgcacaa gcgcaccaat gcggtgctgt
     1201 accggcgcga ggaggccgaa gcctgctggc gcgagatcgt cgcgcccgtc ctgtacgtca
     1261 tggcggcgca gtcggaatac ctgccgcgac tcggcgcgga cgggcgaccg gagcacatgg
     1321 cgcgcctcat ccgccgcctc gagccctgcg tgatcgagga cgcgagccac atggtccacc
     1381 acgagcggcc cgaggcgctg gcgcacgcga tcgagtcctt cctcgctgcg acgccatccc
     1441 tataatcggt cgcaccgggg gccgatgcga tgtccgacgg cgcgaatgat ccacgaggct
     1501 ggttcgccga gctgaaacgc cgcaaggtgt ttcgcgtcgc cgcggtgtac ctcgtcgccg
     1561 cgtggctggt ggtgcaggtg gccggcacga ccttcgagcc catgggactg cccgcctgga
     1621 cgctgaagct ggtgatcacg ctggtggtgc tcggattccc cgtggcctgc gcgctcgcct
     1681 gggccttcga cgtgacgccg cgcggcatcg agcgcacgcc agtcgccgcg ccgccgacgg
     1741 ggcttgcgct tgcccacggc tccggcagcc ttgcgccgtc gcacgcagcg ccggccggcg
     1801 acactactcc gaccgcgact ccggtcacgg ctccgatcgg tgggccagcc gcaagtgaat
     1861 ccgccgactc ggtcgccatc ctgccgttcg tggacatgag cccggaacgc gaccaggagt
     1921 acttctgcga cggcatcgcg gaggagatca tcaactcgct gtgctgcatc cgcgacctgc
     1981 gcatcgcgtc gcggacgtcg tcgttccagt tcaaggggcg ggccgcggac gtgcgcgaga
     2041 tcggccggac gctcggcgtc ggcgcggtgc tggaaggcag cgtgcgcaag gcagggtcgc
     2101 gggtgcgcat cacggcgcag ctggtgagct ccacggacgg ctatcacctc tggtcggaga
     2161 gctacgaccg cgagctcgcg gacgtcttcg caatccagac cgagatcgcg cagaagctgg
     2221 ttgccgcgct gcgcgtcacg ctgagccgcg aggagcgcga gctgatccag cgccgcggga
     2281 cgagcaatgc cgaggcctac gacctgtacc tgcgcgggca ggcccacctg agggacggca
     2341 cggacgtcgc gatgggaccg gcgagcgact gcttccgcca ggccatccgg cgggacgagc
     2401 ggttcgctca ggcgcacgcc ggcctcgcca atgcactggc actcaagggc ctgtggcgca
     2461 tcgggatgac gcccgcggat ttcgaggaag catttgcggc cggccggcgc gccctggagc
     2521 tcgagccgcg catgccggag gcgtaccttg cgcaggcgat gctgctctcg atgcaaggcc
     2581 gggccgccga ggccgaccgg tccttcgagg agtcgatccg cctcaacccc gcgtcgttcg
     2641 acgcgcacta catgtacggg aggcactgtt tccagagtgg cgcgttcgac cgcgccgtgc
     2701 c
//
LOCUS       DAOMFD010000252         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_20402_length_2701_cov_34.543084, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000252 DAOMFD010000000
VERSION     DAOMFD010000252.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20402_length_2701_cov_34.543084"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1163)
                     /locus_tag="PL152_10535"
     CDS             complement(<1..1163)
                     /locus_tag="PL152_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQR49763.1"
                     /translation="MDFIRIRGARTHNLQGLDLDLPRDRLIVITGLSGSGKSSLAFDT
                     IYAEGQRRYVESLSAYARQFLSVMEKPDVDHIEGLSPAIAIEQKAASHNPRSTVGTVT
                     EIYDYLRVLFARAGVPRCPEHGQDLAAQTVSQMVDQVLALREGTAVLLLAPVVQDRKG
                     EHAEVIEHLRGQGYVRAYVDGRLVELDAAPALDPRRRHSIDAVVDRLRVRPDAGQRLA
                     ESFETALALSQGVARLAFQDDAKRDPLVFSNRYACTACGYSLAELEPRVFSFNNPSGA
                     CGTCGGLGVQEFFDPARVVVNPSLSLAGGAVRGWDRRNAYYFQLIQSLARHYRFDIET
                     SWNDLPEAVRHVLLWGSGEERVEFRYFDARGGSARRSHAWEGILPNLERRYRET"
     gene            1128..2450
                     /locus_tag="PL152_10540"
     CDS             1128..2450
                     /locus_tag="PL152_10540"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR49764.1"
                     /translation="MGARASDADEVHAGVRNSAADGQPANIGRAREATPISTPVETAR
                     PALTPHERRSVGALAAIYATRMLGLFLLLPVLALYARQLPDYTPELLGLTMGAYGLTQ
                     ALLQIQFGRWSDRYGRKPVITVGLVLYAAGSLIGAFAHSVAILFLARCVQGAGAVSAA
                     VTALLADLTRPEVRTRAMAFVGISIGLSFVLALVLAPVLDAVVGVPGIFVIMLAMALT
                     GLVLLHVAVPVEPVRTGGAEARQLAEMKDILTRPELRPLYAGVFALHFIMTATFLSVP
                     QVLATDLGIAIATHWKVYLGVFVASLVGTVPLILATERGGRGGLVFVAAVLLVGCAQG
                     LLGLDHRHLWRVLAALTLFFAVFNYLEARLPALLTQAAPGTDRGAALGIFATAQFLGA
                     FCGGALGGILLGQFGLSGVFWGSAVVAVAWAAVAAAGGTGRPIRHGHA"
     gene            2517..>2701
                     /gene="ssb"
                     /locus_tag="PL152_10545"
     CDS             2517..>2701
                     /gene="ssb"
                     /locus_tag="PL152_10545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HQR49765.1"
                     /translation="MARGINKVILVGNLGKDPETRYMPNGKAVTNFSIATSESWKDKQ
                     TGEQREQTEWHNIVMYDR"
BASE COUNT          382 a          935 c          949 g          435 t
ORIGIN      
        1 gtctcgcggt agcggcgctc gaggttgggc aggatgcctt cccaggcgtg gctgcgccgc
       61 gcgctgccac cgcgcgcgtc gaagtaccgg aactcgacgc gctcctcgcc gctgccccag
      121 agcagcacgt ggcgcacagc ctccggaagg tcgttccacg acgtctcgat gtcgaagcga
      181 tagtggcgcg ccagcgactg gatcagctgg aagtagtacg cgttgcggcg atcccagccg
      241 cgcaccgcgc cacccgccag cgacagcgac ggattgacca ccacgcgcgc gggatcgaag
      301 aactcctgca cgccgagccc gccacaggtg ccgcacgctc cgctcgggtt gttgaacgag
      361 aacacgcgcg gctcgagttc ggcgaggctg tagccgcacg ccgtgcacgc atagcgattg
      421 gagaaaacga gcgggtcgcg cttcgcgtcg tcctggaacg cgagtcgcgc gacgccctgc
      481 gacagcgcga gcgccgtctc gaacgattcc gccagacgct gcccggcgtc cggccggacc
      541 cgcagccggt cgacgacggc gtcgatggag tggcggcgtc gtgggtcgag cgccggcgcg
      601 gcgtcgagtt cgaccagccg gccgtcgacg taggcgcgta cgtagccctg tccacgcagg
      661 tgctcgatca cctcggcgtg ctcacccttg cggtcctgca cgacgggcgc gagcagcagc
      721 accgcagtgc cttcccgcag ggcgagcacc tggtccacca tctggctcac cgtctgtgcg
      781 gccaggtcct ggccgtgctc cggacagcgc ggcacgccgg cgcgggcgaa cagcacccgc
      841 aggtagtcgt agatctcggt gacggtcccg accgtcgagc gcggattgtg cgaggcggcc
      901 ttctgctcga tcgcgatcgc gggcgagagg ccctcgatgt ggtccacgtc gggcttttcc
      961 atgaccgaca ggaactggcg ggcgtaggcc gacagcgatt ccacgtagcg gcgctggccc
     1021 tccgcgtaga tggtgtcgaa ggcgagcgag gactttcccg agcccgacag gccggtgatc
     1081 acgatcaggc ggtcgcgcgg caggtcgagg tcgagcccct ggaggttgtg ggtgcgcgcg
     1141 cctcggatgc ggatgaagtc catgcgggtg tccggaattc ggcggccgac gggcagcctg
     1201 ctaatatagg ccgagcccga gaggcaacgc caatcagtac cccagtcgaa actgccaggc
     1261 cggcgctcac gccgcacgag cgccggtccg taggcgcgct tgcggccatc tacgccacgc
     1321 ggatgctggg gctgttcctg ctgctcccgg tgctggcgct ctatgcccgg caactgcccg
     1381 actacacgcc ggaactgctc ggtctcacca tgggagccta cggactgacg caagcgctgc
     1441 tgcagatcca gttcggccga tggtcggacc gctatggtcg caagcccgtg atcaccgtcg
     1501 gactcgtgct ctacgctgct ggctcgctga tcggcgcctt cgcccacagc gtcgcgattc
     1561 tcttcctcgc gcgctgcgtc cagggggccg gcgcggtctc cgcggccgtg accgccctgc
     1621 tcgcggacct gacgcggccg gaggtgcgca cgcgggcgat ggcgttcgtc ggcatcagca
     1681 tcggcctgtc gttcgtgctg gcgctggtgc tggctcccgt gctcgacgcc gtggtcgggg
     1741 tgccggggat cttcgtgatc atgctggcca tggcgctgac cggcctcgtc ctgctgcacg
     1801 ttgcagtgcc ggtcgagccg gtgcgcacgg gcggcgcgga ggcgcggcaa ctcgcggaaa
     1861 tgaaggacat cctgacgcgc ccggagctga ggccgctgta cgcgggtgtc ttcgcgctgc
     1921 acttcatcat gacggccacc ttcctgtcgg tgccgcaggt gctcgcgacc gacctcggca
     1981 tcgcgatcgc cacgcactgg aaggtctacc tcggcgtgtt cgtggcgtcg ctcgtcggga
     2041 ccgtgcctct catccttgcg accgagcgcg gcggccgggg cgggctggtg ttcgtggcgg
     2101 ccgtgctgct ggtcggctgc gcccagggac tgctcgggct cgaccaccgc cacctgtggc
     2161 gcgtgctcgc cgcgctgacc ttgttcttcg ccgtgttcaa ctacctcgag gcgcgcctgc
     2221 ccgcgctgct cacgcaggcg gccccgggga cggaccgcgg ggccgcgctc gggatcttcg
     2281 ccacggcgca gttcctcggg gccttctgcg gtggtgccct gggcgggatc ctgctcggac
     2341 agttcggcct gtccggggtc ttctggggca gcgccgtcgt ggcggtggcc tgggcggcag
     2401 tcgccgccgc gggtggcacc ggccggccga ttcggcacgg acacgcctga ttcgcggtta
     2461 tcgccccggc accccggtgc cgtaaaatcc cattcctttt cacgcaggta ggcgccatgg
     2521 cacgcggtat caacaaagtc atcctcgtcg gcaatctcgg caaggacccg gagacgcgct
     2581 acatgccgaa cggcaaggcg gtcaccaact tcagcatcgc cacctccgag agctggaagg
     2641 acaagcagac cggcgagcag cgcgagcaga ccgagtggca caacatcgtc atgtacgacc
     2701 g
//
LOCUS       DAOMFD010000253         2689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_20558_length_2689_cov_37.582384, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000253 DAOMFD010000000
VERSION     DAOMFD010000253.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20558_length_2689_cov_37.582384"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..554)
                     /locus_tag="PL152_10550"
     CDS             complement(<1..554)
                     /locus_tag="PL152_10550"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: protein motif:HMM:NF012699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HQR49766.1"
                     /translation="MQNETMTRAAEHAPVNWVTTILFTILPLAALVLVPWYGFAHGYS
                     VASVVLFVVLLWAGGISITAGYHRLWSHRTYSAHWTVRLFFMLFGAMSLQNSILIWSS
                     QHRTHHRFVDDWDKDPYSARRGFWFSHMGWILRDYPSGVNDFSNARDLEREPMVMFQH
                     RYYLPLTLFMNIAFPLLLGWAVGDV"
     gene            complement(653..1105)
                     /locus_tag="PL152_10555"
     CDS             complement(653..1105)
                     /locus_tag="PL152_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation protease"
                     /protein_id="HQR49767.1"
                     /translation="MSDVAPVLVLAVGNPSRGDDAIGPVLAARIEAAGWPGVEVITEF
                     QLQVENALDLVGRERTLFIDAGTGTPGPLELRKITPLVPGAAYTSHALSPEAVLGTYA
                     RVQAESPPESWILCVRGERFELGEGLSPDAARNVEAAWEATRAWLAAE"
     gene            complement(1102..2568)
                     /locus_tag="PL152_10560"
     CDS             complement(1102..2568)
                     /locus_tag="PL152_10560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011516268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HQR49768.1"
                     /translation="MSLAELETARNPDDLRRVVIEPLSRVEGHGKVTLLMDEDGRVQQ
                     ARLHIVEFRAFEKFIQGRPYWEVPVMVQRLCGICPVSHHLAASKALDQVVGAVHLTPT
                     AEKIRRLMHYGQILQSHALHFFHLSSPDLLFGFDSEVARRNIVGVAAAYPDVARQGVL
                     LRKYGQEVIRHTAGKRIHGTGSVPGGVNKSLTVAERDDLKRDIYQVVAWSRDAVRLVR
                     QLFEASLEEYRVFGTFRAHSLCLVRADGAMDLYHGGLRARDADGRRIFDHVDYTRYWD
                     QISEEVKAWSYMKFPFLRALGPEDGWYRVGPLTRVTRCDFIPTPLADRERREFLAFDD
                     GRAALATLGFHWARMIEMLHAAESIKDLLHDDDLLGHELMAGGERQARGVGVIEAPRG
                     TLIHHYRVDENDQVARANLIVSTTHNNQAMNRAIREVARRYLDGRELTEGLLNHIEVA
                     VRAFDPCLSCATHAMGKMPLAVELVDADGAVVDRLVRG"
BASE COUNT          441 a          917 c          907 g          424 t
ORIGIN      
        1 acgtcgccca cggcccagcc gagcagcagc gggaaggcga tgttcatgaa cagcgtgagc
       61 ggcaggtagt accggtgctg gaacatcacc atcggctcgc gctcgaggtc gcgcgcgttg
      121 gagaaatcgt tcacaccgct cgggtagtcg cgcaggatcc agcccatgtg cgagaaccag
      181 aagccgcgcc tggcgctgta cgggtccttg tcccagtcgt ccacgaagcg gtggtgggtg
      241 cggtgctgcg acgaccagat caggatgctg ttctgcaagg acatcgcccc gaacagcatg
      301 aagaacagcc gaaccgtcca gtgcgcgctg taggtgcggt gcgaccacag ccggtggtag
      361 cccgcggtga tcgagatgcc gcccgcccag agcaggacga cgaacagcac gaccgacgcc
      421 acgctgtagc cgtgcgcgaa gccgtaccac ggcacgagca ccagggccgc gagcggcagg
      481 atcgtgaaga gaatggtcgt gacccagttg accggcgcgt gttccgcggc gcgagtcata
      541 gtttcgtttt gcatcagtgg gttaccggcg agccgtcagt aaaaaaggcg taccagttta
      601 ccctcggggc ctggcacccg ctggacaatc gcaacctttt gatatgaaag acttattcgg
      661 ctgcgagcca ggcgcgagtc gcctcccatg cggcctcgac gttccgggcc gcatccggcg
      721 acaggccctc ccccagctcg aagcgctctc cccggacgca caggatccac gattcaggtg
      781 gcgattcggc ctgcacgcgg gcgtaggtcc cgagtaccgc ctccggcgac aacgcgtggg
      841 aggtgtacgc ggcgcctggc accaacggcg tgatttttct caattcaagc ggcccgggcg
      901 tgccggtgcc ggcatcgatg aacagggtcc tctcgcgacc tacgaggtct agcgcgttct
      961 cgacctggag ctggaactcg gtgatgacct cgacgccggg ccagccggcc gcctcgatgc
     1021 gcgccgccag cacggggccg atcgcatcgt caccgcggct cggattgccc acggcgagca
     1081 cgagcacggg ggcgacatcg ctcatccgcg caccagccgg tcgacgaccg cgccgtcggc
     1141 atcgacgagc tcgacggcga gcggcatctt gcccatcgcg tgcgtggcgc acgacaggca
     1201 cggatcgaat gcccgcacgg cgacctcgat gtggttcagc agcccttcgg tgagctcgcg
     1261 gccgtcgaga tagcgtcgcg cgacctcgcg gatcgcgcgg ttcatcgcct ggttgttgtg
     1321 ggtcgtcgag acgatcaggt tggcgcgcgc tacctggtcg ttctcgtcca cgcgatagtg
     1381 gtggatgagc gtaccgcgcg gtgcctcgat cacgccgacg ccgcgggcct gccgctcgcc
     1441 gccggccatc agctcgtggc cgagcaggtc gtcgtcgtgc agcaggtcct tgatcgactc
     1501 cgcggcgtgc agcatctcga tcatgcgcgc ccagtggaag ccgagcgtgg caagcgcggc
     1561 gcggccgtcg tcgaaggcga ggaattcccg ccgctcgcga tcggcgagcg gcgtcgggat
     1621 gaagtcgcac cgggtgactc gcgtgagcgg accgacgcga taccagccgt cctccgggcc
     1681 gagtgcgcgc aggaaaggga acttcatgta cgaccaggcc ttcacttcct cggagatctg
     1741 gtcccagtag cgcgtgtaat ccacgtggtc gaagatccga cgaccgtccg cgtcgcgcgc
     1801 gcgcaggccg ccgtggtaga ggtccatcgc cccgtccgcg cgcaccaggc agagcgagtg
     1861 ggcccggaac gtgccgaaca cgcggtactc ctcgaggctg gcctcgaaca gctggcgcac
     1921 cagccgcacc gcatcgcggc tccacgccac gacctggtag atgtcgcgct tcaggtcgtc
     1981 gcgctcggcg accgtcaggc tcttgttcac gccgcccggc accgagccgg tgccgtggat
     2041 gcgcttgccc gccgtgtgcc ggatgacctc ctgcccgtac ttgcgcagca gcacgccctg
     2101 ccgcgcgacg tccggatacg ccgccgcgac gccgacgatg ttgcggcgcg cgacctccga
     2161 gtcgaacccg aacagcaggt ccggtgacga gaggtggaag aagtgcagcg cgtgcgactg
     2221 caggatctgc ccgtagtgca tcagccgccg gatcttttct gccgtcggcg tcagatgcac
     2281 cgctcccacc acctggtcga gcgccttcga cgccgcgagg tggtggctca ccgggcagat
     2341 gccgcagagc cgctgcacca tcaccggcac ttcccagtac ggccggccct ggatgaactt
     2401 ctcgaacgcg cggaactcga cgatgtgcag ccgcgcctgc tgcacgcggc cgtcctcgtc
     2461 catgagcagc gtgaccttgc cgtggccctc gacgcgcgac agcggctcga tcacgacgcg
     2521 tcgcaggtcg tcgggattgc gtgcggtttc cagttcagcg aggctcatgc gtgtccaccg
     2581 ctccccgtca gtcgtagctg atgaggccgt ggccgagctt cggcgtgcgg ccctcgacca
     2641 ggtccatgag gaatttccag atcgcgtcgc ccgacggcgg gcagcccgg
//
LOCUS       DAOMFD010000254         2670 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_20820_length_2670_cov_32.519694, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000254 DAOMFD010000000
VERSION     DAOMFD010000254.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2670)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20820_length_2670_cov_32.519694"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(17..1060)
                     /gene="gmd"
                     /locus_tag="PL152_10565"
     CDS             complement(17..1060)
                     /gene="gmd"
                     /locus_tag="PL152_10565"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HQR49769.1"
                     /translation="MNTRKSAVVTGITGQDGAYLAQLLLDRNYKVYGTYRRTSSVNFW
                     RIEELGIQDHPNLEVVEYDLTDIGTSIALVQRTQPDEIYNLAAQSFVGVSFDQPTTTA
                     HITGLGALNLLEAVRLVNPKTRFYQASTSEMFGKVQAVPQDESTPFYPRSPYGVAKLF
                     AHWMTVNYRESYGIFGASGILFNHESPLRGREFVTRKITDGAARIKLGKLDVLELGNM
                     DAKRDWGYAKEYVEGMYRMLQAPEPETFVLATNRTETVRDFVRMAFKAVDIELDFRGS
                     GVDEQGIDAKSGKTLVRVNPKFYRPAEVDLLIGNPAKAKAKLGWEPRTTLEELCRMMV
                     EADLRRNIRGVSF"
     gene            1292..>2670
                     /locus_tag="PL152_10570"
     CDS             1292..>2670
                     /locus_tag="PL152_10570"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HQR49770.1"
                     /translation="MLAIILCGGSGTRLWPVSREAMPKPFMRIGDGPSLLQRTALRSR
                     RLGADSCLVVTNGEYTFKTIEEFTELGEKAPADLQLLLEPVGRNTAPAIAAACCVLEK
                     QGRTHEPLIILPADHLIQDEARFAEVAGHAARLAGEGRIVLFGIHPTLPETGFGYIEC
                     AAVPVGDAAQPVRRFVEKPSPEKATEYVAAGNYLWNSGMFCFTAGTMLAALERHAPTV
                     LTAARAAVAQGTTPSLQPQRLQLDRVAFQSAPDISIDYAVMERESNVVVLAGDFGWSD
                     IGSWKAVAEEFVRDAGGNTTSGEVVLADCRNVHAQTEDRLVAAVGLKDVVIVDTPDAV
                     LVCDRHASQKVKDVVNELKRRGHDTHKLHRTVARPWGTYTTLQEGPNFKIKRIEVKPG
                     ASLSLQMHHHRSEHWVVVSGTGKVTVDDKVVLLAPNESTYVPVGAKHRLENPGRVPLT
                     LIEVQCGDYV"
BASE COUNT          461 a          874 c          876 g          459 t
ORIGIN      
        1 cagcacacgg ccggcgtcag aacgagacgc cacggatgtt ccgccgaaga tccgcctcga
       61 ccatcatccg gcacagctct tccagcgtcg tgcggggctc ccagcccagc ttggccttgg
      121 ccttcgccgg gttcccgatc agcagatcca cctccgccgg ccggtaaaac ttgggattga
      181 cccgaaccag cgtcttgccg ctctttgcat cgatcccctg ctcgtcgact ccgctgccgc
      241 ggaagtcgag ttcgatgtcc accgccttga atgccatgcg gacgaagtcg cggaccgtct
      301 cggttcgatt cgtggccagc acgaacgtct ccggctcggg cgcctgcagc atgcggtaca
      361 tgccttcgac gtactccttc gcgtagcccc agtcgcgctt ggcgtccatg ttgccgagct
      421 cgaggacgtc cagtttcccg agcttgatcc tggcggcccc gtccgtgatc ttccgtgtca
      481 cgaattcgcg accgcgcaac ggcgactcat ggttgaagag gatcccgctg gcgccgaaga
      541 tgccgtagct ctcgcggtaa ttcactgtca tccagtgcgc gaagagcttg gcgacgccat
      601 acggactgcg cggatagaac ggcgtggact cgtcctgggg cacggcctgc accttgccga
      661 acatttccga cgtcgacgcc tggtagaagc gcgtcttcgg attgaccagc cgaaccgcct
      721 ccagcaggtt caacgcgccc agaccggtga tgtgggctgt tgtcgtcggc tggtcgaatg
      781 aaacgccaac gaagctctgc gcggcgaggt tgtagatctc gtccggctgc gtgcgctgga
      841 ccagggcgat gctggtgccg atgtcggtca ggtcgtactc gaccacctcg aggttggggt
      901 ggtcctggat gcccaattcc tcgatgcgcc agaagttcac cgagctggtc ctgcggtagg
      961 tgccgtagac cttgtagttg cgatccagca ggagctgagc caggtaggct ccgtcctgcc
     1021 ccgtgatccc ggtgacgacc gctgacttgc gcgtgttcat gggtgaggtc tggtgaggta
     1081 gttcattgcc tggcgcccgg gctcaagcac aaccctgcag ctagtctcga aacggcatcg
     1141 aatccgagtc ccagctgggg aagtcagcct ggcctggcta agcaccagcg gtcgatagaa
     1201 tgccggctgg tgcagattgt aacgagcgcc ggagccacgg cagcctgagc gtgggggcct
     1261 gcgctcatgg caatggggag cataccgaag catgttggcg atcattctgt gcggcggatc
     1321 cggaacgcga ctatggccag tgtcgcgcga ggccatgccc aagccgttca tgcggattgg
     1381 cgacggccct tcgctgctcc agcgaaccgc cctgcgttcc cgccgcctgg gtgccgactc
     1441 ctgcctggtc gtgaccaacg gcgagtacac cttcaagacg atcgaggaat tcaccgagct
     1501 cggagagaag gcaccggctg acctgcagct gctgctcgag ccggtcggtc gcaatacggc
     1561 gcccgccatc gccgccgcct gctgcgtgct ggaaaagcag ggtcgcaccc atgagccgct
     1621 gatcatcctg ccggccgacc acctgatcca ggacgaggcc cgcttcgccg aagtggcggg
     1681 ccacgcggcg cgcctggccg gcgagggccg cattgtgctg ttcggcatcc accccactct
     1741 gcccgagact ggcttcggct acatcgagtg cgcggcggtg cccgtcggcg atgccgcgca
     1801 gcccgtgcgt cgcttcgtcg agaaaccgtc gccggaaaag gcgaccgagt atgtcgccgc
     1861 cgggaactac ctgtggaaca gcggcatgtt ctgcttcacg gccggcacga tgctggccgc
     1921 gctggagcgg cacgccccga ccgtgctgac cgccgcacgg gcagccgtcg cgcagggcac
     1981 gacgccgtcg ctgcaaccac agcgcctgca actcgaccgc gtcgcgttcc agtcggcgcc
     2041 ggacatctcg atcgactatg cggtgatgga acgcgagtcg aacgtcgtcg tgctggcggg
     2101 agacttcggc tggagcgaca tcggctcctg gaaggccgtg gcggaggagt tcgtgcgcga
     2161 cgcgggcggc aacaccacca gcggggaagt cgtgctcgcc gactgccgca atgtgcacgc
     2221 ccagaccgaa gaccggctgg tggcggcagt ggggctcaag gatgtcgtta tcgtcgacac
     2281 gccggacgcc gtgctggtct gcgaccgaca tgcctcgcag aaggtcaagg acgtcgtcaa
     2341 cgagctcaag cggcgcggcc acgacactca caagctgcac cgcaccgtgg cgcggccctg
     2401 gggcacctat acgacgctgc aggaaggccc gaacttcaag atcaagcgga tcgaggtaaa
     2461 gccgggcgcc tcgctgtcgc tgcagatgca ccaccatcgc agtgaacact gggtggtggt
     2521 cagcggaacc ggcaaggtca cggtcgacga caaggtcgtg ctgctggcgc ccaacgaatc
     2581 gacgtacgtt ccggtgggcg ccaagcatcg cctggaaaac cccgggcgcg tgccgctgac
     2641 gctgatcgaa gtgcaatgcg gcgactacgt
//
LOCUS       DAOMFD010000255         2651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_21098_length_2651_cov_40.947227, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000255 DAOMFD010000000
VERSION     DAOMFD010000255.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21098_length_2651_cov_40.947227"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PL152_10575"
     CDS             complement(<1..617)
                     /locus_tag="PL152_10575"
                     /inference="COORDINATES: protein motif:HMM:NF024912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49771.1"
                     /translation="MCGIFGAVALGGGVLAHEGLFPEMAASLHHRGPDGSRLLRSARC
                     ALGASRLRIVDPTEQADQPFHTPDGNSWLGCNGEIYNAGALRRRYASYPYRSRTDIET
                     ILPAYRELGVEVFGELDGMFAIAIWDAATGTLVLARDRAGEKPLFYARIGDELWFASE
                     MQALLLHPGLDRAIDETAVSQYLRLGYPLEPRTLFAGIRRVESGTA"
     gene            complement(622..2373)
                     /locus_tag="PL152_10580"
     CDS             complement(622..2373)
                     /locus_tag="PL152_10580"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HQR49772.1"
                     /translation="MSAPSLATATRALPESRARLLVIACWFLLILASMFAWLGSYPLF
                     EPDEGRNAEVAREMVATGDFTVPHLNGLPYVDKPALYFSVVATSLRAFGLDEFAARLP
                     SLIFTIITALMIGWFGRRLWNSRVGAAAAAMTLSAPLAFGMSRVVILDSLFSMLVVGS
                     LVGLYLAIEARAAASAAQHASGGSRRWSSFAWACIGLGILTKGPVALMIPLAAGIPYA
                     VRRRALPAVLFGPGVILASIVVAPWVVQMSQALPGYLHYVLVTETWARVSSDQLHRSE
                     PFWFFVPVLVVGTMPWIGAAVAGVWSAIRGRSRTRLDHRLVFLALWVVVPFVLFSLSR
                     SKLPHYMLPLVPAVALLAAAFMFDDTKPVPTGRPSGSVMLVTFGLAAIGAALFLRAGV
                     RLDATALAAARTFFSIYGAAALAAGVAGLVAKPRGAALVAVFSLPMIVAGFASGPLLK
                     EAADDDSAREFARIITQRVPDHPIVIGVRAFPTSLPFYLRGPVQLSSTSGIESTSNYV
                     HAKFDELLAAPGSTLHDADWWRHELEDCRQQTFFVVQLINSTAADRLTKAGLPLAATS
                     RRYALYGPCQPVARTAE"
     gene            complement(2430..>2651)
                     /locus_tag="PL152_10585"
     CDS             complement(2430..>2651)
                     /locus_tag="PL152_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49773.1"
                     /translation="HREALDVGRIIESHHAALAAIAAPGRNGATASVTQEEDLVRDYR
                     LELEKARGMLEALDAALSKLLTSLVRHGI"
BASE COUNT          473 a          900 c          923 g          355 t
ORIGIN      
        1 gccgtgccgc tctccacgcg tcggattccg gcgaacaggg tgcgcggctc gagcggatag
       61 ccgaggcgca ggtactgcga cacggccgtc tcgtcgatcg cgcggtccag gccaggatgc
      121 aggagcagcg cctgcatctc cgaggcgaac cagagctcgt cgccgatgcg tgcgtagaac
      181 aacggcttct cgccggcgcg gtcgcgggcc agcacgagcg tgccggtcgc ggcgtcccag
      241 atggcgatcg cgaacatccc gtccagttcg ccgaacacct cgacgcccag ctcgcggtac
      301 gcgggcagga tcgtctcgat gtcggtgcgc gaccggtacg gatagctggc gtagcgccgc
      361 ctcagggccc ccgcgttgta gatctcgcca ttgcagccca gccagctgtt gccatccggc
      421 gtgtggaagg gctggtcggc ctgctctgtg ggatcgacga tgcgcagccg gcttgcgccc
      481 agcgcgcagc gggccgagcg cagcaggcgc gatccgtcgg gcccccggtg atgcagcgac
      541 gcagccatct ccgggaacag gccctcatgg gccagcacgc caccgccgag cgccaccgcg
      601 ccgaaaatgc cacacatggg tctactccgc cgtgcgggcc accggctggc acggcccgta
      661 cagcgcatat cggcgactgg tcgctgcgag cggcagcccc gccttggtca gccgatccgc
      721 cgcggtcgag ttgatcagct gcaccacgaa gaacgtctgt tgccggcagt cctcgagctc
      781 gtgccgccac cagtcggcgt cgtgcaatgt cgagccggga gcggcgagca actcgtcgaa
      841 cttcgcgtgc acgtaattgc tggtgctctc gatgccgctc gtgctcgaca gctggaccgg
      901 cccgcgcagg taaaacggca gtgacgtggg gaacgcgcgc actccgatga cgatcggatg
      961 gtccggtacg cgctgcgtga tgatcctggc gaactcccgc gcggagtcgt cgtcggctgc
     1021 ttccttgagc aacggtcccg aggcgaaacc ggcgacgatc atgggcagcg agaacacggc
     1081 gaccagcgcc gcgccgcgcg gtttcgcgac gagcccggct acgcctgcgg cgagcgcagc
     1141 cgctccgtag atggagaaga acgtgcgcgc ggcagccagg gcggtggcgt ccagtcgcac
     1201 gccggcgcgc aggaacagcg cggcgccgat cgccgcgaga ccgaacgtga ccagcatcac
     1261 cgatccggac ggcctgccgg tgggcaccgg cttcgtgtcg tcgaacatga acgcggcggc
     1321 gaggagcgca accgccggaa ccagcggcag catgtagtgc ggcagcttcg agcgggagag
     1381 cgagaacagc acgaacggga cgacgaccca tagcgcgaga aacacgagac gatggtcgag
     1441 cctagttcgc gatcgcccgc ggatcgcact ccagacgccc gcaactgccg cgccgatcca
     1501 tggcatcgtc cccacgacca gcaccggaac gaagaaccag aacggctcgc tgcggtgcag
     1561 ctggtcgctg gagacgcgcg cccatgtctc cgtgaccagc acgtagtgca ggtagcctgg
     1621 caatgcctgc gacatctgca cgacccaggg cgcgacgacg atgctcgcga gaatcacgcc
     1681 aggcccgaac aacaccgcgg gcagagcgcg gcgtcgcacg gcgtacggga ttccggccgc
     1741 cagcggaatc atcagtgcga cggggccctt ggtcagtatg ccgaggccga tgcaggccca
     1801 tgcgaacgac gaccagcgac gcgagccgcc ggatgcgtgc tgcgccgccg acgctgcagc
     1861 acgtgcttcg attgcgaggt agaggccaac cagggatccc acgaccagca tcgagaacag
     1921 cgagtcgagg atgacgacgc gcgacatgcc gaaggccagc ggcgcggaca acgtcatggc
     1981 tgctgccgcc gcgccgaccc gggaattcca cagtcgccgg ccgaaccagc cgatcatcag
     2041 cgccgtgatg atcgtgaaga tcagcgatgg caaccgcgcg gcgaactcgt ccagcccgaa
     2101 agcgcgcagc gatgtagcga ccacggagaa gtacagggcc ggcttgtcga cgtatggcaa
     2161 cccgttcaga tgcggaacgg tgaaatcgcc ggtcgcaacc atttcccgcg cgacctcggc
     2221 gttgcggcct tcgtccggct cgaacagcgg gtagctgccg agccacgcga acatcgaggc
     2281 caggatcagc aggaaccagc acgcgatgac gagcaggcgc gcgcgactct cgggcagcgc
     2341 gcgagtggcg gtagcgaggc tcggcgcaga cattggatgc gttccgggct gggcggtggg
     2401 tggagccgcg ccggcgccgc cgctaaggtt cagattccgt gccgcaccag gctcgtgagc
     2461 agcttggaga gcgcggcgtc cagcgcctcg agcattccgc gggccttctc cagctccagc
     2521 cggtagtcgc ggaccaggtc ttcctcttgc gtcacgctcg ccgtcgcacc gttgcgcccg
     2581 ggagccgcga tcgcagcgag cgccgcgtga tgtgactcga tgatgcgacc gacgtcgagg
     2641 gcttcgcggt g
//
LOCUS       DAOMFD010000256         2649 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_21130_length_2649_cov_32.115652, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000256 DAOMFD010000000
VERSION     DAOMFD010000256.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2649)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2649
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21130_length_2649_cov_32.115652"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /locus_tag="PL152_10590"
     CDS             complement(<1..284)
                     /locus_tag="PL152_10590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HQR49774.1"
                     /translation="MDRSTVGEYLRARGVSRRDFLKFCTTAATVMALPASMAPRIAAA
                     LDKARRPSVIWLPFQECTGCTESITRSHGATIEGMIFDAISLDYQHTLQAA"
     gene            complement(338..700)
                     /locus_tag="PL152_10595"
     CDS             complement(338..700)
                     /locus_tag="PL152_10595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HQR49775.1"
                     /translation="MSGSSAARAAAPAFVVDRATIKRLEKNAARACDFLGAMANPSRL
                     LILCQLAEGEKAVGELQPLIGLSQSALSQHLALLREKKLVRTRRDGQQIFYSLASHEA
                     QALMHTLHEEFCAPKRKA"
     gene            complement(697..1137)
                     /locus_tag="PL152_10600"
     CDS             complement(697..1137)
                     /locus_tag="PL152_10600"
                     /inference="COORDINATES: protein motif:HMM:NF013743.2"
                     /note="part of multienzyme complex composed of H, L, P,
                     and T proteins which catalyzes oxidation of glycine to
                     yield carbon dioxide, ammonia, 5,10-CH2-H4folate and a
                     reduced pyridine nucleotide; protein H is involved in
                     transfer of methylamine group from the P to T protein;
                     covalently bound to a lipoyl cofactor; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HQR49776.1"
                     /translation="MPTVKGCNLPDDLLYDVENQIWFRELGDGTVKLGMTAVATAMAG
                     KLVAYTPKSVGKDVKAGKSCATVESGKWVGPAKTAAAGTVVAVNDALSANPSLANSDP
                     YGEGWLLVLKPEEWDSVKGTLVPGAQVAGPYEAKMNADGFAGCG"
     gene            complement(1150..1611)
                     /locus_tag="PL152_10605"
     CDS             complement(1150..1611)
                     /locus_tag="PL152_10605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49777.1"
                     /translation="MRPLVDIERRLDQLTGNWEMGTFSNFESKLENVPAELLELGEEV
                     LEHWVAAKGLEPTQELREGFRLLALHAQGTKGDPSFNACRETCRELVYHYNLLTMDPQ
                     HEESPRRLRMMALVANHLRLFVVGKLQVAGLGDFCCSSRPAREQAARVEPA"
     gene            complement(1608..2543)
                     /locus_tag="PL152_10610"
     CDS             complement(1608..2543)
                     /locus_tag="PL152_10610"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoB--CoM heterodisulfide reductase iron-sulfur
                     subunit B family protein"
                     /protein_id="HQR49778.1"
                     /translation="MSAAKGKYQKVAYYPGCALEGTAHSYNRSTKEVGKALGLDLVEV
                     ENWNCCGAMEVKNIDPKLQTYLSSRVMSIAATEMKMDVVMAPCNGCYHNLKKAEYDLA
                     HDPKSVEVVGQLSKKAGHDAYKAGSVETIHALDWIKHGIGEDGLKQRVRGGLKGLKVA
                     NYYGCMYVRPRHIFPEKDQGPGSESTSKPHFMDDLLAAAGAVNVEFPLKTACCGGAHT
                     LSDSDTSTKLVLNILRAAEAAGAEVIATECPTCHSGLEMHQVRAEKVLGQATSVKILY
                     FTQLIGLALGLSPRQVGVHENISDSLGFLKERGIA"
BASE COUNT          378 a          909 c          888 g          474 t
ORIGIN      
        1 gcggcctgca gcgtgtgctg gtagtcgagg gagatcgcgt cgaagatcat tccctcgatc
       61 gtcgcgccgt gcgagcgcgt gatcgactcg gtgcagccgg tgcactcctg gaacggcagc
      121 cagatgacgg acggacgccg cgccttgtcg agcgccgccg cgatccgcgg agccatcgac
      181 gcaggcagcg ccatgacggt ggcggccgtg gtgcagaact tgaggaagtc gcgacgtgag
      241 acgccgcgcg cgcgcaggta ctcgccgacg gtgcttcgat ccatagcgcc ctccgctcga
      301 ggcggtaccg cgcccagggc acgcggctcg ccgcaggtca ggccttgcgt ttcggcgcgc
      361 agaactcctc gtgcagcgtg tgcatgagcg cctgcgcctc gtggctcgcg aggctgtaga
      421 agatctgctg gccgtcgcgg cgcgtgcgca cgagcttctt ctcccgcagc agcgcgaggt
      481 gctgcgacag cgccgactgg ctgagcccga tcagcggctg cagttcgccc acggccttct
      541 cgccctcggc gagctggcag aggatgagga gtcgcgacgg gttcgccatc gcaccgagga
      601 agtcgcaggc gcgcgcagca ttcttctcga gccgcttgat cgtggcgcgg tcgacgacga
      661 acgccggggc cgcggccctc gcggcgctgg agccgctcat ccgcaacccg cgaagccgtc
      721 ggcgttcatc ttcgcctcgt acggcccggc gacctgcgcg cccggcacca gcgtgccctt
      781 gacgctgtcc cattcctcgg gcttcagcac cagcagccag ccctcgccgt acgggtcgga
      841 gttcgcgagg ctcgggttcg cgctgagcgc gtcgttcacg gcgaccacgg ttccggcagc
      901 ggcggtcttg gcgggtccga cccacttgcc ggactcgacg gtggcgcagg acttgccggc
      961 cttgacgtcc ttgccgacgc tcttcggcgt gtaggcgacg agcttgccgg ccatggcggt
     1021 cgcgaccgcg gtcatgccga gcttcaccgt gccgtcaccg agttcccgga accagatctg
     1081 gttctcgacg tcgtagagca ggtcgtcagg aaggttgcag cctttcaccg tgggcatggt
     1141 cgggtctcct caggccggtt cgacgcgggc ggcctgctcg cgcgccgggc gcgaggagca
     1201 gcagaagtcg ccgagcccgg cgacctgcag cttgccgacc acgaaaagtc gcagatggtt
     1261 ggccacgagc gccatcatcc gcaggcggcg aggggattcc tcgtgctgcg gatccatcgt
     1321 cagcaggttg taatggtaga cgagttcgcg gcaggtttcc cggcacgcgt tgaagctcgg
     1381 gtcgcccttc gtgccttggg cgtgcagggc gagcaggcgg aagccttcgc gcagctcctg
     1441 cgtcggctcg aggcccttgg ccgcgaccca atgctcgagg acctcttcgc caagctcgag
     1501 caactcggcg gggacattct ccaatttcga ctcgaaattg gagaatgtcc ccatttccca
     1561 atttccggtc agctggtcga gccgacgctc gatgtcgacg agcggtctca cgcgatgcca
     1621 cgctccttga ggaacccgag cgagtcggag atgttctcgt gcacgccgac ctggcgcggc
     1681 gagagcccta gcgcgaggcc gatgagctgg gtgaaataga ggatcttcac gctcgtcgcc
     1741 tggcccagca ccttctccgc gcgcacctgg tgcatctcga ggcccgagtg gcaggtcggg
     1801 cactcggtcg cgatgacctc cgcgccggcg gcttcggcag cgcgcaggat gttcagcacc
     1861 agcttcgtcg acgtgtcgct gtcggacagc gtgtgggcgc cgccgcagca ggcggtcttg
     1921 agcgggaact cgacgttcac ggccccggcc gccgcgagca ggtcgtccat gaagtgcggc
     1981 ttggaggtgg actcgctgcc ggggccctgg tccttctccg ggaaaatgtg gcgcggccgc
     2041 acgtacatgc agccgtagta gttggcgacc ttgagcccct tgagcccgcc gcgcacgcgc
     2101 tgcttcagcc cgtcctcgcc gatcccgtgc ttgatccagt cgagcgcgtg gatggtctcc
     2161 accgagcccg ccttgtacgc gtcgtgtccg gccttcttcg acaactgccc gacgacctcg
     2221 acggacttcg gatcgtgggc gaggtcgtac tccgccttct tgaggttgtg gtagcagccg
     2281 ttgcacggtg ccatcaccac gtccatcttc atttccgtgg ccgcgatcga catcacgcgc
     2341 gaagacaggt acgtctgcag cttcgggtcg atgttcttca cttccatcgc cccgcagcag
     2401 ttccagttct cgacctcgac gagatcgagc ccgagcgcct tgccgacctc cttcgtcgag
     2461 cggttgtacg aatgcgccgt gccctcgagc gcgcagcccg ggtagtaggc caccttctgg
     2521 tacttgccct tcgcggcgct catgactgca ctccatgacc ggcagcggcc ggccctgcat
     2581 cgcgctttgc ggcatcgatc aggccgtcga gcccgagcgc gcgccgccgc ctgccttcct
     2641 gctcctcga
//
LOCUS       DAOMFD010000257         2642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_21222_length_2642_cov_32.490143, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000257 DAOMFD010000000
VERSION     DAOMFD010000257.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2642
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21222_length_2642_cov_32.490143"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            254..931
                     /locus_tag="PL152_10615"
     CDS             254..931
                     /locus_tag="PL152_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HQR49779.1"
                     /translation="MAIELYWGSGSPYSWRVQLALEVKRLPYASHLLQFSKQEHKSPQ
                     MLRMNPRGRVPVLKDGDYIVFESLACLLYLDRKYPDPPIFGRTPEEAGTIMRVICEYQ
                     AYAEAHLMEIVSAVFLGQMDGRLEEVTKASHLVEGEARTIEGRLSKSDWLVGEHVSAA
                     DMVVFPGIMLLLRAMEKREAGDLRSRFLPMGATYPAIARWIGRMESLPGFERTVPPHW
                     REGSPRG"
     gene            1034..2458
                     /locus_tag="PL152_10620"
     CDS             1034..2458
                     /locus_tag="PL152_10620"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HQR49780.1"
                     /translation="MSNYPVHVNFPGRLVFVGFGSIGQGVLPLILRHVGIKADQVTVV
                     TADEAGEKVAAEYGVRFIKHPLTRENFRNVLDPLVGRGDFLLNLSVDVSSVALIQFCW
                     EKGALYLDTCIEPWVGGYTDPTVAPSRRSNYALRETALALRDTVKRAPTAVLTHGANP
                     GLVSHFVKQALLNIATDTGVDHGDPNTRDEWARLAHKLGVKVIHIAERDTQVASHAKE
                     PGEFVNTWSVDGFVGEGCQPAELGWGSHEKNWPRDGKKHDFGRGSAIYLMQPGAATRV
                     RTWTPLAGRFHGFLITHGESISISDYLTVKKGDHVTYRPTVHYAYHPSDDAVLSVHEL
                     AGRNWQIQDRKRIMMDEIVKGVDELGVLLAGHKKNAYWYGSQLSVQEARQLCPHNNAT
                     SLQVTVSVLGGLVWAMENPNAGIVEPDELDFRRILEVCRPYLGLVVGEYTEWHPLVDR
                     GRLFPEDLDETDPWQFKNVRVDWK"
BASE COUNT          432 a          876 c          893 g          441 t
ORIGIN      
        1 cgttcaggtc cgcgtacatc gcaccgggga ggtggcccgc ggcgtacagc gcccggccgg
       61 catccgggcg cgacagttcg aagcggcagt cgaagatgcg gacagtcgca tcctgcagtc
      121 gttgcgccag ttgctccgcc tggatcaagg ttcccatggg tcattcccct gcagtgatac
      181 cgtcgtccgg tcgccgtcag tacaatccgg acgcctcgcc cgctgcgtcc gggcggcggc
      241 acggaggcgc gcgatggcga tcgagctcta ctggggcagc ggcagcccgt attcgtggcg
      301 cgtgcagctc gcgctcgagg tcaagcgcct gccgtacgcg tcccacctgc tgcagttttc
      361 caagcaggag cacaagtcgc cgcagatgct gcgcatgaat ccgcgcggcc gtgtgccggt
      421 cctcaaggac ggcgactaca tcgtcttcga atcgctcgct tgcctgctct acctggatcg
      481 caagtacccc gacccgccga tcttcggccg cacgccggag gaagccggaa cgatcatgcg
      541 cgtgatctgc gagtaccagg cgtatgccga ggcgcacctc atggagatcg tgtcggcggt
      601 gttcctcggc cagatggacg ggcgtctcga ggaagtcacg aaggcgagcc acctggtcga
      661 gggcgaggcc cggaccatcg agggcaggtt gagcaaatcg gactggctgg tgggcgagca
      721 cgtctcggcc gcggacatgg tggtgttccc ggggatcatg ctactgctcc gggcaatgga
      781 aaagcgcgag gccggcgacc tgcgctcgcg attcctgccg atgggtgcga cctacccggc
      841 catcgcgcgc tggatcggcc gcatggagtc gctgcccggc ttcgaacgca ccgtgccgcc
      901 gcactggcgc gaaggatctc cgcgcggctg aggccgggcg ggtcgacccg ctcggaccgg
      961 cggggtgacc cgggaataat tacgggtaca cttatcgtcc ttccgtcgtt ccatcccgtt
     1021 cctcggtgtc gaaatgagca attacccggt tcacgtcaat tttcccgggc ggctggtgtt
     1081 tgtcggcttc ggcagtatcg ggcaaggcgt cctgccgctg atcctgcgtc acgtcggaat
     1141 caaggccgac caggtcacgg tcgtcacggc cgacgaggcg ggtgagaagg tggcggcgga
     1201 gtacggggtg cgcttcatca agcatccgct cacccgcgag aatttcagga acgtcctcga
     1261 cccgctggtc ggtcgcggtg atttcctgct gaacctctcg gtggacgtct cgagcgtcgc
     1321 gctgatccag ttctgctggg agaagggcgc gctgtacctc gatacctgca tcgagccgtg
     1381 ggtcggcggc tacacggacc cgacggtcgc gccgtcccgc cgctccaact acgcgctgcg
     1441 cgagacggcg ctggcgttgc gcgacaccgt caagcgcgcg ccgacggcgg tgctcacgca
     1501 cggcgccaac ccggggctcg tgtcgcactt cgtcaagcag gcgttgctca acatcgcgac
     1561 cgacaccggc gtcgatcacg gcgacccgaa cacgcgcgac gagtgggcgc gcctcgcgca
     1621 caagctcggc gtcaaggtga tccacatcgc cgagcgcgac acgcaggtcg catcccatgc
     1681 gaaggagccg ggcgagttcg tcaacacctg gtcggtggac ggcttcgtgg gcgaggggtg
     1741 ccagccggcc gaactcggct ggggctcgca cgagaagaac tggccgcgcg acggcaagaa
     1801 gcacgacttc ggccgcggca gcgcgatcta cctgatgcag ccgggcgcgg cgacgcgcgt
     1861 gcgcacgtgg acacctctcg cgggccggtt ccacggcttc ctgatcaccc acggcgagtc
     1921 catctcgatc tcggactacc tcaccgtgaa gaagggcgac cacgtgacct atcgcccgac
     1981 ggtgcactac gcctaccacc cgagcgacga tgccgtcctg tccgtgcacg agctggccgg
     2041 gcgcaactgg cagatccagg accgcaagcg gatcatgatg gacgagatcg tgaagggcgt
     2101 ggacgaactc ggcgtcctgc tcgcgggcca caagaagaac gcctactggt acggctcgca
     2161 gctgtccgtg caggaagccc ggcagctgtg cccgcacaac aacgccacgt cgctgcaggt
     2221 caccgtctcg gtgctcggcg gcctcgtctg ggcgatggag aacccgaatg ccggcatcgt
     2281 cgagcccgac gagctcgact tccgccgcat cctcgaggtc tgccgtccgt acctcgggct
     2341 cgtcgtgggc gaatacacgg aatggcaccc gctcgtcgat cgcggccgcc tgttccccga
     2401 ggacctcgac gagaccgatc cctggcagtt caagaacgtc cgggtggact ggaagtagga
     2461 aacaggggac attctcctat ttccgctttc ggaaatagga gaatgtcccc taattcttcg
     2521 gtgggtggtg gctgcctctg cggcgcggtg cgtctgcgcg ccgtgctccc gccaaactgg
     2581 gtcgcgcact gtcactgctc gatgtgccgc cgcgcgcacg gcgcggggtt cgtgacctgg
     2641 gt
//
LOCUS       DAOMFD010000258         2624 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_21478_length_2624_cov_34.668743, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000258 DAOMFD010000000
VERSION     DAOMFD010000258.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21478_length_2624_cov_34.668743"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..882)
                     /locus_tag="PL152_10625"
     CDS             complement(1..882)
                     /locus_tag="PL152_10625"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HQR49781.1"
                     /translation="MHDKWTGPELDSLLGKSEFTRRAMIVTSLAAGFAAAVRPVRADT
                     VITTDTQGLTAGEIKIPVADGEIPAYRARPAAGAKLPVVLVVQEIFGVHEHIRDLCRR
                     FAKEGYLAIAPELYARQGDPSQYTDWKQLYADIVMKVPDAQVMSDLDAAVAWAGKSGE
                     GDPGRVGITGFCWGGRIVWLYAAHSVALKAGVAWYGRLTGEATPLQPKYPLDLAAELK
                     APVLGLYGGKDQGIPLTDVDKMRAALAAARSPSEIVVFPDAEHGFNADYRPSYRAEDA
                     KEGWRRCLAWFRKYGVG"
     gene            complement(906..1388)
                     /locus_tag="PL152_10630"
     CDS             complement(906..1388)
                     /locus_tag="PL152_10630"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HQR49782.1"
                     /translation="MNWDLPGPFIHERVAHHAEIDGYGHVNNVCYVSWMDDCAWAHST
                     ARGIPPEMCRRLNRGMVVWRTQINYLGAAYEGDHIQVATWPVMNDGKLRIERRFQIRR
                     ESDGQTLVRALIHYVCMDLTTGRAKRMPDEFTKYTVAPEVAAAIAAETEPFQPGVEPR
                     "
     gene            complement(1385..2287)
                     /locus_tag="PL152_10635"
     CDS             complement(1385..2287)
                     /locus_tag="PL152_10635"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF010621.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HQR49783.1"
                     /translation="MQERKGSTLKGVLTISLMTLNVIAWCIVLFTVAIAKFLVPIPAW
                     RRVSSRAMTGLAEGWIGTNNALFRALGSLPLAATGLDDLSTREWYLVVSNHRSWVDIL
                     VLQAVFNRRIPFLKFFLKQQLIWVPFLGIAWWALDFPFMRRHSPAYLEKHPEKRGEDL
                     AVTRRACEKFRLIPTSVMNFVEGTRYTKAKHEAFKSPYRHLLPPRPGGTSFVLSAMGG
                     MLHSMLDVTIAYSSESPSLWDLCCGRLGTVTVDVRRRPIDEWMAAGNYADDPAFRGRF
                     KDWLGGLWAEKDALLDGMLAGADP"
     gene            2362..>2624
                     /locus_tag="PL152_10640"
     CDS             2362..>2624
                     /locus_tag="PL152_10640"
                     /inference="COORDINATES: protein motif:HMM:NF016240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF480 domain-containing protein"
                     /protein_id="HQR49784.1"
                     /translation="MSPELTALEARVIGCLIEKQIATPDQYPLSLNALTAACNQKSNR
                     HPVLDLEERDVQETLDGLARRQLVMEKSGFGSRVPKYQHLFCNT"
BASE COUNT          433 a          915 c          858 g          418 t
ORIGIN      
        1 ctagccgacg ccgtacttgc ggaaccacgc gaggcaacgc cgccagcctt ccttcgcatc
       61 ctcggcgcga tagctcgggc ggtaatccgc gttgaagcca tgctccgcgt ccgggaacac
      121 gacgatttcc gacgggctcc tggccgcggc gagcgccgcg cgcatcttgt cgacgtccgt
      181 gagcggaatc ccctggtcct tgccgccgta cagcccgagc accggggcct tgagttcggc
      241 ggcgaggtcg agcggatatt tcggttgcag aggtgtcgcc tcgccggtca atcggccata
      301 ccaggccacg cccgccttca aggccacgct gtgggccgcg tagagccaca cgatgcggcc
      361 tccccagcag aagcccgtga tcccgaccct gcccgggtca ccctcgccgg acttcccggc
      421 ccatgcgacg gccgcatcca ggtcggacat cacctgggca tccggcacct tcatgacgat
      481 gtcggcgtac agctgcttcc agtccgtgta ctgcgacggg tcgccctgcc tcgcgtagag
      541 ttccggcgcg atggcgagat aaccctcctt ggcgaaacga cggcacaggt cgcggatgtg
      601 ctcgtgcaca ccgaagatct cctgcacgac gagcacgacc ggaagcttcg cccccgccgc
      661 tggtcgcgcg cgatacgcgg gaatctcgcc gtcggcgacc gggatcttga tttcgccggc
      721 cgtgaggccc tgggtgtcgg tcgtgatcac cgtgtccgcg cgcaccgggc gcaccgccgc
      781 cgcaaacccg gcggcgagcg acgtcacgat catggcgcgg cgcgtgaact cgctcttgcc
      841 gagcaatgaa tcgagctccg ggcctgtcca cttgtcgtgc atggcgtctc ctcctggtgg
      901 tcgtctcagc gcggctccac gccgggctgg aagggttcgg tctcggctgc gatcgccgcg
      961 gccacctcgg gcgcgacggt gtatttcgtg aactcgtctg gcatgcgctt ggccctgccg
     1021 gtggtgaggt ccatgcagac gtagtgaatg agcgcgcgca ccagcgtctg cccgtccgac
     1081 tcacggcgga tctggaagcg gcgctcgatg cgcagcttgc cgtcgttcat cacgggccag
     1141 gtggccacct ggatgtgatc cccctcgtag gcggcgccga ggtagttgat ctgcgtgcgc
     1201 cacaccacca tgccgcgatt caggcgccgg cacatttccg gcggaatccc gcgcgcagtc
     1261 gagtgagccc acgcacagtc gtccatccag gagacgtagc agacattgtt gacgtggccg
     1321 tagccgtcga tctccgcgtg gtgcgccacg cgctcgtgga tgaacggccc gggcaggtcc
     1381 cagttcatgg atccgcaccg gccagcatgc cgtcgagcaa tgcgtccttc tccgcccaca
     1441 ggccgccgag ccagtccttg aaccggccgc gaaacgccgg gtcgtcggca tagttgcccg
     1501 ccgccatcca ctcgtcgatc ggccgccggc gtacgtcgac cgtcaccgtc ccgaggcggc
     1561 cgcagcacag gtcccacaga ctcgggctct cgctcgagta ggcgatggtg acgtcgagca
     1621 tcgagtgcag cattccgccc atggcggaca gcacgaacga ggtcccgccc gggcgcggcg
     1681 gcagcaggtg ccggtaaggc gacttgaacg cctcgtgctt ggccttcgtg tagcgcgtgc
     1741 cctccacgaa gttcatcacc gaggtcggga tcagccggaa tttctcgcac gcgcgacggg
     1801 tgaccgcgag gtcctcgccc cgcttctccg gatgcttctc gaggtaggcc ggggaatggc
     1861 ggcgcatgaa cggaaagtcg agtgcccacc acgcgatgcc gaggaacggc acccagatca
     1921 gctgctgctt gaggaagaac ttgaggaacg ggatgcgccg gttgaacacc gcctgcagca
     1981 cgaggatgtc gacccacgag cggtggttgg acaccacgag gtaccactcg cgggtggaca
     2041 ggtcatcgag cccggtcgcc gcgagcggca gcgaacccag cgcgcggaac aaggcgttgt
     2101 tggtgccgat ccagccctcg gccaggcccg tcatcgcgcg gctcgatacc cgccgccagg
     2161 cggggatcgg caccaggaat ttcgcgatcg cgacggtgaa cagcacgatg caccacgcga
     2221 tgacgttcag cgtcatcagc gagatcgtga gcacgccctt cagggtcgag cctttgcgct
     2281 cctgcacgag gtgacatccg ctggccgggt gggcgattat gccctgccgg ggcctgtgaa
     2341 ccccagccca cggagcgcgc gatgtccccg gaactcaccg ccctcgaagc ccgcgtgatc
     2401 ggctgcctca tcgagaagca gatcgcgacc ccggaccagt accccctgtc gctgaacgca
     2461 ctcacggccg cgtgcaacca gaagagcaac cgccacccgg tgctcgacct cgaggaacgc
     2521 gacgtgcagg agacgctcga cgggctcgcg cgccggcagc tcgtcatgga gaagtcgggg
     2581 ttcggcagcc gcgtgcccaa gtaccagcac ctgttctgca acac
//
LOCUS       DAOMFD010000259         2618 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_21568_length_2618_cov_29.271947, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000259 DAOMFD010000000
VERSION     DAOMFD010000259.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21568_length_2618_cov_29.271947"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..397
                     /locus_tag="PL152_10645"
     CDS             <1..397
                     /locus_tag="PL152_10645"
                     /inference="COORDINATES: protein motif:HMM:NF016854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HQR49785.1"
                     /translation="RVEPAPRSRQMAVSEDFLTYVQEQLAGIDGLSSRRMFGGVGLYS
                     GDRFFGLLADNTLYLKADDLDRGDFLARGMQPFRPYRNRPELSMSYYAVPADVLEDAD
                     TLLAWARRAVRSAVASPGRGKPRRGRAKF"
     gene            438..1277
                     /locus_tag="PL152_10650"
     CDS             438..1277
                     /locus_tag="PL152_10650"
                     /EC_number="3.4.15.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0044265 - cellular macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanophycinase"
                     /protein_id="HQR49786.1"
                     /translation="MPSKVPEGASRGWIVPIGGAENKENDRHILERFVHVAGGADADI
                     VVIPTASRLAETGPRYERLFRDIGAGQVTSMDFDTRRDCQEPRRLQRLEHATGIFFTG
                     GNQLRLTTLLGGTPVAKLIRVRNANGVAVGGTSAGASILSEHMIAFGDEGSSVISGSV
                     RLAPGLGLTNRFIIDQHFRQRDRLGRLLTALAYNPFAVGIGLDEDTAAFIGPDETVEV
                     EGSGGVTVVDASDVSYSSMDAVSEGQPVCMLGLKLHILVAGATFNLHTRLAAAGTVVQ
                     PKE"
     gene            1403..1846
                     /locus_tag="PL152_10655"
     CDS             1403..1846
                     /locus_tag="PL152_10655"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HQR49787.1"
                     /translation="MGLSAATIWWVAAGVTVAAELATGTFYLLMIALGLAAGAITAHL
                     GFSESAQVACAAIVGGGATALWHWRRARHPRSAPAAENRDVNLDIGERVKVTEWAPDG
                     TSRVSYRGSFWPARIAHGATAAPGEHEIVAVEGNRLVLAPRIQRN"
     gene            1871..>2618
                     /locus_tag="PL152_10660"
     CDS             1871..>2618
                     /locus_tag="PL152_10660"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045121 - membrane raft [Evidence IEA];
                     GO_process: GO:0034982 - mitochondrial protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="stomatin-like protein"
                     /protein_id="HQR49788.1"
                     /translation="MSIALILAVIAAIFIIQTFKIVPQQHAWVVERLGRYDRTLTPGL
                     KFVVPFVERVAYKHSLKEVPLDVPSQVCITKDNTQLQVDGIIFYQVTDPMRASYGSSN
                     YVVAITQLAQTTLRSVIGKMELDKTFEERDAINASVVAALDEAAANWGVKVLRYEIKD
                     LTPPNEILRAMQAQITAEREKRAVIATSEGKMQEQINLATGERQAFIARSEGERQAAI
                     NKAQGEAAAITTVANATADAIRKIAEAIRSP"
BASE COUNT          449 a          891 c          900 g          378 t
ORIGIN      
        1 gcgagtcgag cccgcgccgc ggagccggca gatggccgtc agtgaagact tcctgaccta
       61 cgtccaggag caactggccg gcatcgacgg gctgagctcc aggcgcatgt tcggcggcgt
      121 cggactgtac tcgggtgaca ggttcttcgg cttgcttgcc gacaacacgc tctatctcaa
      181 ggcggacgat ctcgatcgag gtgattttct cgcgcggggc atgcagccgt tccgcccgta
      241 tcgcaaccga cccgagctct cgatgagtta ttacgcagtc cccgccgacg tgctggagga
      301 cgcggatacg ctgctggcat gggcgcggcg ggccgtgcgc tcagctgtcg cctcgcccgg
      361 ccgcggcaag ccgcgccgcg gccgggcgaa attctgagac aatcccgacc cggtacccga
      421 caaaggccgc accaggcatg cccagcaagg ttcccgaagg cgcatcgcgc ggctggatcg
      481 tcccgatcgg cggcgcggag aacaaggaga acgaccggca catcctcgag cgcttcgtgc
      541 atgtcgcagg cggcgcggac gccgacatcg tcgtcattcc cacggcgagt cgccttgccg
      601 agaccggccc gcgttacgaa cggctgttcc gcgacatcgg cgccggacag gtcacgtcga
      661 tggacttcga cacgcggcgc gactgccagg agccgcggcg cctgcagcgt ctcgagcacg
      721 ccaccggcat attcttcacg ggaggcaacc agctcaggct cacgacgctg ctcggcggga
      781 cacccgtggc gaagctgatc cgggtgcgca atgccaacgg cgtcgccgtg ggcggcacca
      841 gcgccggtgc gagcatcctg agcgagcaca tgatcgcctt cggcgacgag ggatcgtcgg
      901 tcatctccgg cagcgtgcgg ctcgcgcccg gcctcggcct caccaaccgg ttcatcatcg
      961 accagcactt tcgccagcgc gaccggctcg gacgactgct caccgcactc gcctacaatc
     1021 ccttcgccgt cggcatcggc ctcgacgagg acacggccgc gttcatcggc ccagacgaga
     1081 ccgtcgaggt cgaaggcagc ggaggcgtga cggtcgtgga cgcttccgac gtcagctact
     1141 cgtcgatgga tgccgtgagc gaggggcagc cggtctgcat gctgggcctg aagctgcaca
     1201 tcctggtggc tggcgcgacg ttcaacctgc acacgcgcct ggccgccgca gggaccgtgg
     1261 tccagccgaa ggagtgacgc ctgctccggg gtggggtgac gagacgcgac accggcgagg
     1321 cggtaggccc caccgtccgg tcgttctgct atcgttgatc ccgacgggcg ccgccggcgc
     1381 ccatgcgacg ggtgactgga cgatgggact gtccgcagcg acgatctggt gggtggccgc
     1441 aggcgttacg gtcgctgccg aactggcgac gggcacgttc tacctgctga tgatcgcgct
     1501 cgggctggcg gccggcgcga tcacggcgca cctcggattc tccgagtcgg cgcaggtcgc
     1561 ctgcgcggca atcgtcggcg gcggcgccac ggcgctgtgg cactggcgtc gcgcgcgtca
     1621 tcctcgctcg gcgccggccg cggagaatcg cgacgtcaac ctcgacatcg gcgagcgcgt
     1681 caaggtgacc gagtgggcgc ccgacgggac gtcgcgcgtc agctatcgcg gcagcttctg
     1741 gcccgcccgc atcgcccacg gggcgacggc ggccccgggc gagcacgaaa tcgtcgcggt
     1801 ggagggcaac cgcctggtgc tggcgccgcg catccagcgg aactgagcgc gaggcaaggg
     1861 agacttcgag atgtccatcg cactgatcct cgccgtcatc gcggcgatct tcatcatcca
     1921 gaccttcaag atcgtcccgc agcagcatgc ctgggtggtc gagaggctcg ggcgctacga
     1981 ccgcacgctg acaccgggcc tcaagttcgt cgtgccgttc gtcgagcgag tcgcctacaa
     2041 gcattcgctc aaggaggtgc cgctcgacgt gccgagccag gtgtgcatca ccaaggacaa
     2101 tacgcagctg caggtcgacg gcatcatctt ctaccaggtg accgacccga tgcgcgcgag
     2161 ctacggctcg agcaactacg tggtggcgat cacgcagctc gcgcagacga cgctgcgctc
     2221 ggtcatcggc aagatggaac tcgacaagac cttcgaggag cgcgacgcca tcaatgcgtc
     2281 cgtcgtggca gcgctcgacg aggcggccgc aaactggggc gtcaaggtcc tgcggtacga
     2341 gatcaaggac ctgacgccgc ccaacgagat cctgcgcgcg atgcaggcgc agatcacggc
     2401 ggaacgcgag aaacgcgcgg tgatcgcgac ctcggaaggc aagatgcagg agcagatcaa
     2461 cctcgccacc ggcgagcgcc aggccttcat cgcgcgctcc gagggcgagc gacaggcggc
     2521 gatcaacaag gcgcagggcg aggccgcggc gatcacgacc gtggcgaacg cgacggccga
     2581 cgcaatccgc aagatcgccg aggcgatccg ctctcccg
//
LOCUS       DAOMFD010000260         2616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_21584_length_2616_cov_39.169856, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000260 DAOMFD010000000
VERSION     DAOMFD010000260.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2616
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21584_length_2616_cov_39.169856"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="PL152_10665"
     CDS             <1..180
                     /locus_tag="PL152_10665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49789.1"
                     /translation="MNNGGGFIEVQGTAEGHAFRRHELDRLLDLASAACGKLFAVQLE
                     ALQQPLPGAAPRRGA"
     gene            253..873
                     /gene="rdgB"
                     /locus_tag="PL152_10670"
     CDS             253..873
                     /gene="rdgB"
                     /locus_tag="PL152_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HQR49790.1"
                     /translation="MTTRLPPRLVLATGNPGKLRELRAILAPWKVEVLPLSDFTPSAA
                     DETGTTFVDNALLKARFAARMAGLPAIADDSGLEVDALGGGPGVRSARYAGEGADDAA
                     NNALLLRELGQVPDERRTARYRCAMAYVRSADDPAPIVREASWDGRIGHAPRGNGGFG
                     YDPLFVVASGGRTAAEYSADEKNRVSHRGQALRALVAALGAGERQP"
     gene            870..2063
                     /gene="hemW"
                     /locus_tag="PL152_10675"
     CDS             870..2063
                     /gene="hemW"
                     /locus_tag="PL152_10675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HQR49791.1"
                     /translation="MTSLTAPPLSLYVHLPWCVRKCPYCDFNSHPVPSAGIPERAYLA
                     ALLADLDDSAPAVAGREVVSVFFGGGTPSLFADHAIGAILARLRDLLPVADDVEVTLE
                     ANPGTVERGAFAAYRAVGVNRVSLGAQSFDDRRLAALGRIHVADEVRVAVGELRTAGI
                     DNFNLDLMYGLPAQSPAEAIRDVEQAIELGPAHISHYQLTLEPGTAFERRPPPLPDEE
                     AIFAMQASCQARLADAGYVQYEVSAYARPARRCRHNLNYWTFGDYLGIGAGAHGKVTD
                     ASGGRVIRTERVRQPARYIAAADAGQRSAGERGVDSSELAFEFLLNALRLVEGFDERT
                     FESRTGLDWETVSATVDRARASGLLEQTGAGRWAASHRGRLFLNDLQAMFLPERPRGG
                     QRRGA"
     gene            2429..>2616
                     /gene="rpmE"
                     /locus_tag="PL152_10680"
     CDS             2429..>2616
                     /gene="rpmE"
                     /locus_tag="PL152_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006686216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HQR49792.1"
                     /translation="MKSGIHPEYKTITVTCSCGNTFETRSTLGEDLQIEVCSSCHPFY
                     TGKQKIVDTGGRVDRFRK"
BASE COUNT          401 a          897 c          890 g          428 t
ORIGIN      
        1 atgaacaacg gcggcggttt catcgaggtc cagggcacgg cggaaggcca cgccttccgc
       61 cgccacgagc tcgaccgcct gctcgacctc gcctcggctg cctgcgggaa gctctttgcc
      121 gtgcagctcg aggccctgca gcagccgttg ccgggcgccg cgccgaggcg tggcgcatag
      181 tcgagcgccc gcccgccgtg cggcggcatc gacggtgatc cgatcgctcc ctgcatgccc
      241 gtggttccgc acatgacgac gcgccttccg ccgcgactcg tgcttgcgac cggcaacccc
      301 ggcaagctgc gggaattgcg tgccatcctc gcgccgtgga aggtcgaggt cctgcccttg
      361 tccgacttca cgccttccgc agccgacgag acgggcacta cgttcgtcga caatgcgctc
      421 ctcaaggcac gtttcgccgc gcgaatggcg ggcctcccag cgatcgcgga cgattccggt
      481 ctcgaggttg acgcgctcgg cggcgggccg ggcgtgcgct ccgcgcgcta tgcgggcgag
      541 ggcgccgacg atgccgcgaa caacgccttg ctgctgcgcg agctcgggca ggtgcccgac
      601 gagcgccgga ccgcgcgtta tcgctgcgcg atggcctacg tgcgctccgc ggacgatccc
      661 gcgcccatcg ttcgcgaagc cagctgggac gggcggattg gccacgcgcc gcgcggcaac
      721 ggtgggttcg gctacgaccc gctcttcgtc gtcgccagcg gcggtcgcac tgccgcagaa
      781 tactctgccg acgagaagaa ccgcgtgagc catcgaggcc aggcgctgcg ggcgctcgtc
      841 gcggcactcg gcgcaggcga gcgccagccg tgacgtcgct gaccgcgccg ccgctgtcgc
      901 tctacgtgca cctgccgtgg tgcgtgcgca agtgccccta ttgcgatttc aactcccatc
      961 ccgtcccgtc ggcgggcatt ccggaacgcg cctacctcgc cgcgctgctc gcggacctgg
     1021 acgactctgc accggccgtc gcgggccgcg aggtcgtgtc ggtgttcttc ggtggcggca
     1081 cgccgagcct gttcgcggat catgcgatcg gcgcgatact cgcgcgcctg cgcgacctgc
     1141 tgcccgtcgc ggacgacgtc gaagtgaccc tcgaggccaa tccgggcact gtcgagcggg
     1201 gcgcatttgc tgcgtaccgt gcggtcggcg tcaatcgcgt gtcgctcggc gcccagagtt
     1261 tcgacgatcg gagactcgcg gcgctcggcc gcatccacgt ggccgacgaa gtccgcgtcg
     1321 cggtcggcga gctgcgcacg gcggggatcg acaacttcaa cctcgacctc atgtacgggt
     1381 tgcctgcgca gtccccggcg gaagccatcc gcgacgtcga gcaggcgatc gagctcgggc
     1441 ccgcgcacat ctcgcactat cagctgaccc tggagccggg caccgcgttc gagcgccggc
     1501 cgccgccgct gccggacgag gaggcgatct tcgcgatgca ggcgtcgtgc caggcgcggc
     1561 tcgcagacgc aggttacgtc cagtacgagg tgtcggccta cgcgcggcca gcgcggcgct
     1621 gccgccataa tctcaactac tggacgttcg gggattacct cggtatcggc gccggagcgc
     1681 acggcaaggt gaccgatgcc agcggcggtc gcgtgatccg gaccgagcgc gtgcgccagc
     1741 ccgcccgcta cattgctgca gcggatgcgg ggcagcgcag cgccggggag cgcggcgtcg
     1801 attcgagcga actggccttc gagttcctcc tgaacgcgtt gcggctggtc gagggattcg
     1861 acgagcgcac gttcgaatca cggacgggtc tcgactggga gaccgtctcg gcgacggttg
     1921 accgggcccg cgcgagcggg ttgctggagc agacaggcgc gggacgctgg gccgccagcc
     1981 accggggccg cttgttcctc aatgacttgc aggcgatgtt cctgccggag cgccctcgcg
     2041 ggggccagcg gcgtggtgcc tgagaccggc tggaccgtgg ttttgccgtg gcgcgggtcc
     2101 tgcgggtcgc cagttattca cacccctggg ccgtcatcca agattcgacg ggttacggcc
     2161 tgtagtcgaa gtgacatctt aggcagcgat cgtaaaatgt tgatccgtaa tattaaatta
     2221 ccctgatcaa aatttaacca aagcagaatt gccctcgtct cgcgccacgc gcagccgggg
     2281 cggaggtgat tcgtccacaa agttacccac aggttttgtg gataaccagc ggtggtgcca
     2341 aaaccacagt gagggacgcc ttgtcagtgg ggtgccgcgc tcctatactt cgcgcccttt
     2401 ttccgatctg cctccaagga ccgcggccat gaagagcgga atccaccccg agtacaagac
     2461 gatcaccgtg acctgcagct gcgggaacac gttcgagacc cgctccacgc tgggcgagga
     2521 cctgcagatc gaagtctgct cgagctgcca cccgttctac acgggcaagc agaagatcgt
     2581 ggacaccggc ggccgcgtcg accgcttccg caagaa
//
LOCUS       DAOMFD010000261         2587 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_22018_length_2587_cov_33.275671, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000261 DAOMFD010000000
VERSION     DAOMFD010000261.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22018_length_2587_cov_33.275671"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..905)
                     /locus_tag="PL152_10685"
     CDS             complement(<1..905)
                     /locus_tag="PL152_10685"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HQR49793.1"
                     /translation="MTKQNATAATRTLPILQPLPSVPERIGKYHVIHEVGRGSTGTVY
                     LSHDPFYGRDVAIKVYHATTTDDAEARNARRMFLGEAHMVGKLQHPNIVPIYDAGEEE
                     GRRYVVTEHVHGARTLSAYCRPGSLLPVDQVIGIVYKCAKALHYAHSRGVVHRDIKPS
                     NILLTQDGDVRIVDFGIALVSDSDVSRLEGVAGSPAYMSPEQVQGYELDPRSDLYSLG
                     AVMYEMLCGHRPFRAGGLGKLLRQVVQASPEPLRQMRPELAPELEAVVHRALEKEPGN
                     RYRSGGEFAADLTRMHQQLRSTQVE"
     gene            complement(1022..1975)
                     /gene="thiL"
                     /locus_tag="PL152_10690"
     CDS             complement(1022..1975)
                     /gene="thiL"
                     /locus_tag="PL152_10690"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HQR49794.1"
                     /translation="MALGEFDLIERYFTRRPRRSDVLLGVGDDAALLEVPPGRALVAA
                     TDTLVEGRHFLADAPARSVGHQALAVNLSDLAAMGADPAWALLSLSIPEVREDWLAEF
                     AAGLYALADAHGVELVGGDTVRGPLVVTVQALGFVDPAVALKRSGAQPGDLVYVSGTP
                     GEAAAGLAALRRGARLDEQHPLQHRYLYAEPRLALGRALRGRATAAMDVSDGLLGDLG
                     KLCAASRVGATVELDALPVSAALRAAHGREECEQFVLSGGDDYELLFTLPAAEAGRYE
                     VALRKTLAITRIGRIVAGHAVRCERAGVAVDVASQGYDHFA"
     gene            complement(1987..2448)
                     /gene="nusB"
                     /locus_tag="PL152_10695"
     CDS             complement(1987..2448)
                     /gene="nusB"
                     /locus_tag="PL152_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HQR49795.1"
                     /translation="MARRPDRAARARSLARKFAMQALYQWQLTGQTVAELRNQYATEE
                     GFEGADRDYFAALLQGATAARESLDEQLGGMLDRPVAQLDPVEHAVLLIGLYELREQL
                     DVPYRVVINEAVDLTKRFGATDGHKFVNAVLDRAARAMRATEQAAAARERA"
     gene            complement(2451..>2587)
                     /locus_tag="PL152_10700"
     CDS             complement(2451..>2587)
                     /locus_tag="PL152_10700"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HQR49796.1"
                     /translation="AFGVLTTDTIEQAIERAGTKAGNKGADAAMTALELANLLRRLDV
                     "
BASE COUNT          373 a          924 c          903 g          387 t
ORIGIN      
        1 atctccacct gcgtcgagcg cagctgctgg tgcatgcgcg tcaggtccgc cgcgaattcg
       61 ccgccgctgc gatagcggtt gccgggctcc ttctcgaggg cccggtgcac gacggcctcg
      121 agttcgggcg ccagctccgg tcgcatctgg cgcaacggct ccggcgacgc ctgcacgacc
      181 tgccgcagca gcttgccgag cccgccggcg cggaacggcc ggtgaccgca cagcatctcg
      241 tacatcacgg cgccgagcga atagaggtcg gagcgcggat cgagctcgta gccttgcacc
      301 tgctcgggcg acatgtaggc ggggctgccc gcgacgcctt ccagccgcga gacgtcggaa
      361 tccgacacga gcgcgatgcc gaagtccacg atgcgcacgt cgccgtcctg cgtgagcagg
      421 atgttcgacg gcttgatgtc gcgatggacg accccgcgcg agtgcgcgta gtgcagcgcc
      481 ttggcgcact tgtagacgat gccgatcacc tggtcgaccg gcagcaggct gcccggcctg
      541 cagtaggccg acagcgtgcg cgcgccgtgc acgtgctccg tcacgacgta gcgccggcct
      601 tcctcctcgc cggcgtcgta gatcggcacg atgttcgggt gctgcagctt cccgaccatg
      661 tgcgcctcgc cgaggaacat gcgtcgcgcg ttgcgcgcct cggcatcgtc cgtggtcgtg
      721 gcgtgataga ccttgatcgc gacgtcgcga ccgtagaagg gatcgtgcga caggtacacc
      781 gtaccggtgc tgccgcgccc gacctcgtgg atgacgtggt acttgccgat gcgctccggc
      841 accgacggga gcggctggag gatcggcagg gtccgggtcg ctgcggtcgc gttctgcttt
      901 gtcatcagta ttcgcggacg gccatccaga ggtggccgga ccaccggcca cgatgcagca
      961 gaggatagtg gggaggggtg cggccgttcc gtgaccggag tcccggatcg gtggcattaa
     1021 gtcacgcgaa gtgatcgtat ccctggctgg ccacgtccac ggccacgccc gcgcgttcgc
     1081 agcgtacggc gtgtccggcc acgatccggc cgatccgggt gatcgcgagg gtcttgcgca
     1141 acgcgacttc gtaccgaccg gcctccgccg ccggcagcgt gaacagcaac tcgtaatcgt
     1201 caccgcccga gagcacgaac tgctcgcact cctcgcggcc gtgcgcggcg cgcaacgccg
     1261 ccgaaacggg caaggcatcg agctcaaccg ttgcaccgac cctgctggcc gcgcagagct
     1321 tgccgaggtc gccgagcagg ccgtcggaca cgtccatcgc agcggtcgcg cggccccgca
     1381 gggcgcgacc gagcgcaagt cgcggctccg cgtagagata gcgatgctgc agcgggtgct
     1441 gctcgtcgag tcgcgcacca cggcgcagcg cggcgagccc cgcggctgcc tcgcccggcg
     1501 tgcctgacac gtagaccagg tcgcccggct gtgctccgga acgcttcaga gccaccgctg
     1561 gatcgacgaa cccgagcgcc tgcaccgtga cgacgagcgg cccgcgcacc gtgtcgccac
     1621 cgaccagttc cactccgtgc gcatcggcca gcgcgtagag gccggccgcg aactccgcca
     1681 gccagtcctc gcgcacctcg ggaatcgaca gcgacagcag cgcccaggcc gggtccgcac
     1741 ccatcgcggc caggtcgctc aggttcaccg cgagggcctg gtgaccgacg gaccttgccg
     1801 gcgcgtcggc gagaaagtgc cgtccctcga ccagggtgtc ggtcgcggcg acgagcgccc
     1861 ggcccggcgg cacctcgagc agcgcggcgt cgtcacccac cccgagcagc acgtcggatc
     1921 gccgcggcct gcgcgtgaag taccgctcga tcaggtcgaa ttcgccgagg gccacggcgc
     1981 ccccgctcag gcgcgctcgc gggccgccgc agcctgctcg gtcgcgcgca tcgcgcgggc
     2041 cgcacggtcg agcacggcgt tgacgaactt gtgcccgtcc gtcgcgccga agcgcttggt
     2101 gaggtcgacg gcctcgttga tcacgacgcg gtacggtacg tcgagctgct cgcgcagctc
     2161 gtacaggccg atgagcagga ccgcgtgctc gaccggatcg agctgcgcga ctggccggtc
     2221 gagcatgccg cccagctgct cgtccaggga ttcgcgcgca gcggttgcgc cctgcagcag
     2281 cgcggcgaag tagtcgcgat ccgcgccttc gaagccttcc tcggtcgcgt actggttgcg
     2341 cagctccgcg acggtctggc cggtgagctg ccactggtac aacgcctgca tcgcgaactt
     2401 gcgcgcgagg cttcgcgctc gcgcggcccg gtcggggcgc ctggccatgg tcagacgtcc
     2461 agccggcgca gcaggttggc gagttccagc gcggtcatcg ccgcatcggc acccttgttg
     2521 ccggccttgg tcccggcgcg ctcgatcgcc tgctcgatcg tgtcggtggt gagcacgccg
     2581 aatgcga
//
LOCUS       DAOMFD010000262         2583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_22065_length_2583_cov_34.261867, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000262 DAOMFD010000000
VERSION     DAOMFD010000262.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2583
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22065_length_2583_cov_34.261867"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..268
                     /locus_tag="PL152_10705"
     CDS             <1..268
                     /locus_tag="PL152_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HQR49797.1"
                     /translation="PIPGLPGEAGGNGEEGAGDPEQDPLYDEAVRIVTESRKASISGV
                     QRRLKIGYNRAARMIEAMEAAGLVGPLQSNGSREVLAPPPPEAD"
     gene            276..914
                     /gene="lolA"
                     /locus_tag="PL152_10710"
     CDS             276..914
                     /gene="lolA"
                     /locus_tag="PL152_10710"
                     /inference="COORDINATES: protein motif:HMM:TIGR00547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008320 - protein transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HQR49798.1"
                     /translation="MSRRLLGGLCAGVLACVVLPAAADDSIERVEAWMRSVKTLSADF
                     VQVVRGRGGEITNRASGTLALSRPDRFRWDYQTPNVQVIVADGRKLWLYDADLEQVTV
                     RPLQAGLGSTPAMLLSGAGAVGDSFTAGPVERDGEWTWCRLVPKDRSSDFEGVGLGFD
                     THGVLVAMQLKDKLGQSTELVFSRVKVNSKLDGGLFVFVPPKGADVIGDASK"
     gene            918..1301
                     /locus_tag="PL152_10715"
     CDS             918..1301
                     /locus_tag="PL152_10715"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HQR49799.1"
                     /translation="MLPLRYRGWWIALSAALIAYLIYGSLVPIPPLPMPGNFDKLEHF
                     VVYAGLAIWFTGLFPRSRYGGVVLVLLLIAGSLEIAQGLMHMGRQADVLDMAANATGV
                     GIGLVCALVLTGNWARRVESWLGPR"
     gene            1298..>2583
                     /locus_tag="PL152_10720"
     CDS             1298..>2583
                     /locus_tag="PL152_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016486902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HQR49800.1"
                     /translation="MTDAAVGGWRPLADRMRPASLDEFVGQSHLLAPGKPLRRMLEGR
                     SLHSMILWGPPGTGKTTLARLVARSCDAEFVALSAVMAGVKDVRAAVEQAQGLKRASG
                     RQTVLFLDEVHRFNKSQQDAFLPYVEDGTVCFIGATTENPSFEINSALLSRARVYVLR
                     SLTAEDVRVVLERALQRAGDEDGRSVTIDSDALDTLCRAADGDARRGLNLLEVALDLA
                     RAGDGVITEAIAAEVSAGGYRRFDKKGEQFYDQISALHKSVRGSDPDAALYWLCRMLD
                     GGCDPRYVARRVLRMASEDIGNADPRALTLALEACEAYERMGSPEGELAIAQAVVFMA
                     CAAKSNAVYVAFGAAMEDAKQHGTLDVPLRLRNAPTGLMKGLGYGKGYRYAHDEPDAY
                     AAGEKYFPDEMAPARYYEPVDRGLEIRIREALARLRG"
BASE COUNT          373 a          838 c          971 g          401 t
ORIGIN      
        1 tccgataccc ggattgccgg gcgaggcggg aggcaacggc gaggagggcg ccggcgaccc
       61 ggaacaggac ccgctctacg acgaggccgt gcgcatcgtc acggagagcc gcaaggcctc
      121 catctcgggg gtccagcgcc gcctcaagat cggctacaac cgcgcggccc gcatgatcga
      181 ggccatggag gcggccggcc tcgtgggccc gctgcagtcg aatggcagcc gtgaagtgct
      241 cgccccgccg ccgccggaag cggattgacg cgaacatgag ccgacgcctg ctcggtggcc
      301 tgtgcgcggg cgtgctcgcc tgcgtggtgc tgcccgcggc agccgacgat tcgatcgagc
      361 gtgtcgaggc ctggatgcga tccgtcaaga cgctgtcggc ggatttcgtg caggtcgtgc
      421 gcggacgcgg cggcgagatc acgaatcgcg cgagcggcac gctggcgttg tcgcgaccgg
      481 accgctttcg ctgggactat cagaccccga acgtgcaggt gatcgtcgcc gacgggcgca
      541 agctctggct gtacgacgcg gacctcgagc aggtcacggt gcggcccctg caggcgggac
      601 tgggatccac gccggccatg ttgctgtctg gcgccggcgc ggttggcgac agcttcaccg
      661 ccggaccggt cgagcgcgac ggcgagtgga cctggtgccg gctggtgccg aaggaccgca
      721 gctccgactt cgagggcgtc ggcctcggct tcgacacgca tggcgtgctc gtcgccatgc
      781 agctcaagga caagctcggg cagagcacgg agctcgtgtt ctcccgcgtg aaggtcaatt
      841 cgaagctgga cggcggactg ttcgtcttcg taccgcccaa gggcgccgac gtgatcggcg
      901 acgcgtcgaa gtagcggatg ctgcccttgc gctaccgggg ctggtggatc gcgctgagcg
      961 cggccctcat cgcgtacctc atctatggca gcctcgtgcc gattcctccg ctgccgatgc
     1021 cgggcaattt cgacaagctc gagcatttcg tcgtgtatgc gggcctcgcg atctggttca
     1081 ccggtctgtt cccgcgctca cgctacggtg gggtcgtgct ggtgctgctg ctgatcgccg
     1141 ggagcctcga gatcgcgcag ggactgatgc acatgggccg ccaggccgac gtgctcgaca
     1201 tggcagccaa cgcgacgggt gtcggaatcg ggctcgtgtg cgcgctcgtg ctgaccggga
     1261 actgggcgcg ccgcgtcgag tcgtggctgg gcccgcgatg accgacgcgg cggtgggcgg
     1321 ctggcgcccg ctcgcggacc gcatgcggcc ggcgagcctc gacgagttcg tcgggcagtc
     1381 gcacctgctc gcgcccggca agccgctgcg tcgcatgctc gaggggcgca gcctgcactc
     1441 gatgatcctg tggggtccgc ccggcaccgg caagaccacg ctggcgcggc tcgtcgcgcg
     1501 cagttgcgat gcggagttcg tggcgctgtc cgcggtgatg gcgggcgtga aggacgtgcg
     1561 cgcggcggtc gagcaggcgc aggggctgaa gcgcgcgtcg ggccgccaga cggtgctgtt
     1621 cctcgacgag gtgcaccggt tcaacaagtc gcagcaggac gcgttcctgc cttacgtcga
     1681 ggacggcacg gtctgcttca tcggcgccac caccgagaac ccgtcgttcg agatcaacag
     1741 tgcgttgctg tcgcgtgcgc gcgtgtacgt gctgcggtcg ctcacggccg aggacgtgcg
     1801 ggtagtgctc gagcgggcgc tgcagcgcgc tggcgacgag gacggccgct ccgtcacgat
     1861 cgattcggac gcgctcgaca cgctgtgccg ggcggccgac ggtgacgcgc gacgcggctt
     1921 gaacctgctc gaggtcgccc tcgacctcgc gcgcgcgggc gacggcgtga tcaccgaggc
     1981 cattgccgcg gaagtgtcgg cgggtggtta ccgtcggttc gacaagaagg gcgagcagtt
     2041 ctacgaccag atctcggcgc tgcacaagtc ggtgcgggga tcggatcccg atgctgcgct
     2101 gtactggctg tgccggatgc tggacggcgg ctgcgacccg cgatacgtgg cgcggcgcgt
     2161 gctgaggatg gcgtccgagg acatcggcaa cgcggacccg cgcgcgctga cgctggcgct
     2221 cgaggcgtgc gaggcgtacg agcgcatggg cagtccggaa ggtgagctcg cgatcgccca
     2281 ggcggtcgtg ttcatggcgt gcgccgcgaa gagcaatgcg gtctacgtcg cattcggcgc
     2341 ggcgatggag gatgcgaagc agcacggcac gctggatgtg ccgctgcgcc tgcgcaacgc
     2401 tccgaccggg ctcatgaagg ggctcggcta cggcaagggc taccgttacg cgcacgacga
     2461 gccggatgcc tacgccgccg gggagaagta cttcccggac gagatggcac cggcccgtta
     2521 ctacgagccc gtcgaccgcg ggctcgagat ccgcatccgc gaggcgctcg cgcgactccg
     2581 cgg
//
LOCUS       DAOMFD010000263         2582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_22082_length_2582_cov_37.901464, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000263 DAOMFD010000000
VERSION     DAOMFD010000263.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22082_length_2582_cov_37.901464"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2562
                     /gene="uvrA"
                     /locus_tag="PL152_10725"
     CDS             <1..2562
                     /gene="uvrA"
                     /locus_tag="PL152_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HQR49801.1"
                     /translation="ARDSIVVHKAREHNLRDVDVAIPRDGMTVVTGVSGSGKSTLAFD
                     IVFAEGQRRYLESLNAYARQFVQPAGRPDVDAIFGIPPTVAIEQRTSRGGRKSTVGTL
                     TEVHHFLRLLYVKLGTQHCPDCDVPIEPQSADAIVARLLREARGRRIGLLAPLVVARK
                     GYYTDLARWAAGKGHEHLRVDGEYVPTRPWPRLDRFREHTIELPVGEVVVSSRTERQV
                     RDAVQRALDFGKGVIHVLDRGRVRVFSTRRACPSCGRSFAEPDPRLLSYNSKHGWCTD
                     CYGTGLAIKGFDEEQSGEEIWWNEWFEGEAAPCPACDGRRLNPEALAVRFRDRSIADL
                     STLPVDSSLQFIVRLKLAGREREIARDLLAEIRSRLAFLQDVGLGYIALDRAAPTLSG
                     GEAQRIRLAAQLGSNLRGVCYVLDEPTIGLHPRDNAVLLNALGQLGSKGNTLLVVEHD
                     EDTIRRADHVIDLGPGAGARGGRVVGQGRVADLVAAPESLTGRFLASPLRHPLGERRA
                     VKRGDPKLVIERAALHNLRKVNAGIPLGRLTVVTGVSGSGKSTLARDVLYANLRARSG
                     AGRRGVRRWIGCDGISGIDAVERVLEVDQTPIGRTPRSCPATYVGFWDDIRRLMAGTT
                     DARVRGFGPSRFSFNTAGGRCDACEGQGVQTIEMSFLPNVKVLCDACGGRRFNAETLA
                     IQYRGRSVGDLLAMCVDDAVEFFTAHRRIHQALKLLQDVGLGYLTLGQQSPTLSGGEA
                     QRIKLVTELARLRSGQRTLYVLDEPTVGLHMADVEKLVHVLHRLVDAGNTVVVIEHNL
                     DVIAEADWIVDLGPEGGSGGGRIVAQGAPEQVAKATAKSHTARVLKSFLAERTRR"
BASE COUNT          384 a          884 c          934 g          380 t
ORIGIN      
        1 gcgcgggatt cgatcgtcgt gcacaaggca cgcgagcaca acctgcgcga cgtcgacgtc
       61 gcgataccgc gcgacggcat gaccgtcgtg acgggcgtgt cgggctcggg caagtcgacg
      121 ctcgccttcg acatcgtgtt cgcggagggg cagcgccgct acctggagtc gctcaacgcc
      181 tacgcgcgac agttcgtgca gcctgcgggc cggcccgacg tggacgcgat cttcggcata
      241 ccgcccacgg tggccatcga gcagcgcacc agtcgcggcg gtcgcaagag cacggtcggc
      301 acgctgaccg aggtgcatca cttcctgcgc ctgctctacg tgaagctcgg tacccagcac
      361 tgtccggact gcgacgtgcc gatcgagccg cagagcgcag atgcgatcgt ggcgcgactt
      421 ctgcgtgaag ctcgagggcg gcgcatcggg ctgctcgcgc cgctggtcgt cgcgcgcaag
      481 ggctactaca ccgatctcgc caggtgggcg gcggggaaag gccacgagca cctgcgcgtc
      541 gacggcgagt acgtgccgac gcggccgtgg ccccggctcg accggttccg cgagcacacc
      601 atcgagctgc ctgtcggcga agtcgtcgtc agctcccgga ccgagcgcca ggttcgcgat
      661 gcggtgcagc gggcgctcga cttcggcaag ggcgtgatcc acgtgctcga tcgcggacgg
      721 gtccgcgtgt tctcgacccg gcgggcctgt ccgtcgtgcg ggcgcagctt cgccgagccg
      781 gatccacggc tcctgtccta caactcgaag cacggctggt gcacggactg ctacggcacc
      841 gggctcgcca tcaagggttt cgacgaggag cagagcggcg aggagatctg gtggaacgaa
      901 tggttcgaag gcgaagcggc gccgtgcccg gcctgcgacg gccggcgatt gaatccggag
      961 gcgctcgccg tgcggttccg cgaccgctcg atcgcggacc tgtcgacgct ccctgtcgat
     1021 tcctcgctgc agttcatcgt caggctcaag ctcgccggcc gcgaacgcga gatcgcgcgc
     1081 gacctgctgg ccgagatccg ctcgcgcctc gccttccttc aggacgtcgg ccttggctac
     1141 attgcgctcg atcgcgccgc acccacgctg tcgggtggtg aggcgcagcg catccgcctc
     1201 gcagcgcagc tcggctcgaa cctgcgcggc gtgtgctacg tgctcgacga gccgaccatc
     1261 ggcctgcacc cgcgcgacaa cgccgtgctg ctgaacgcgc tcgggcagct cgggtcgaag
     1321 ggcaacaccc tgctcgtcgt cgagcacgac gaggacacga tccgccgtgc cgatcacgtg
     1381 atcgacctcg ggcccggggc cggggcgcgc ggcgggcgcg tcgtggggca gggccgcgtc
     1441 gcggacctcg tggctgcgcc ggaatcgctg accggcaggt tcctcgcatc gccgctgcgg
     1501 catccgctcg gcgagcgccg cgccgtcaag cgcggcgacc cgaagctcgt gatcgaacgc
     1561 gcggcattgc acaacctgcg caaggtgaac gccggcattc cgctcgggcg gctgaccgtc
     1621 gtgaccggtg tctccggatc gggcaagtcc acgctggcgc gggacgtcct gtatgcaaac
     1681 ctgcgcgcgc gatcgggcgc cggtcgtcgc ggcgtcaggc gatggattgg atgcgacggc
     1741 atctcgggca tcgacgcggt ggagcgcgtg ctcgaagtgg accagacgcc gatcggccgc
     1801 acgccgcgct cctgccccgc gacctacgtc ggattctggg acgacatccg ccgcctgatg
     1861 gcggggacga cggacgcacg ggtgcgcggc ttcgggccgt caaggttctc gttcaatacg
     1921 gccggcggcc gctgcgacgc ctgcgagggg cagggcgtgc agacgatcga gatgagcttc
     1981 ctgccgaacg tgaaggtcct gtgcgacgcc tgtggcgggc gcaggttcaa cgccgagacc
     2041 ctggccatcc agtaccgggg caggagcgtc ggcgacctgc tggcgatgtg cgtcgacgat
     2101 gccgtggaat tctttaccgc ccatcgccgc atccaccagg cgctgaagct gctgcaggac
     2161 gtcgggctcg gctacctgac gctcggccag cagagcccga cgctctcggg aggggaggcg
     2221 cagcgcatca agctggtgac ggagctggcg cggctgcggt ccgggcagcg cacgctgtac
     2281 gtgctcgacg agccgacggt cggcctgcac atggcggacg tcgagaagct cgtgcacgtg
     2341 ctgcatcgcc tcgtcgacgc aggcaacacc gtcgtcgtga tcgagcacaa cctcgacgtg
     2401 atcgcggagg ccgactggat cgtcgacctc gggcccgaag gcggctcggg cggcgggcgc
     2461 atcgtggcgc agggcgcgcc ggagcaggtc gcgaaggcca cggcgaagtc ccacacggcg
     2521 cgggtgctca agtccttcct cgccgagcgc acgcggcgct gagcacggat gcgcgacgtc
     2581 ac
//
LOCUS       DAOMFD010000264         2550 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_22552_length_2550_cov_34.838477, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000264 DAOMFD010000000
VERSION     DAOMFD010000264.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2550)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2550
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22552_length_2550_cov_34.838477"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..>2550)
                     /gene="ppdK"
                     /locus_tag="PL152_10730"
     CDS             complement(101..>2550)
                     /gene="ppdK"
                     /locus_tag="PL152_10730"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HQR49802.1"
                     /translation="ILNLGLNETTLAALIRRTGNERFGYDAWRRFVQLFGKVALGVPD
                     ALFDEQFEAVKSRAGVKHDVMLGPDELRDVGDRFLAVVQRHTGRPFPSDPYEQLEIAI
                     KAVFNSWMGKRAIDYRREFRITPAMANGTAVNVVTMVFGNMGNDSATGVGFTRNPATG
                     ENQLYGEYLTNAQGEDVVAGIRTPRPIAEMAGEMPAIHAQLVELRNRLERHYREVQDF
                     EFTVERGTLYCLQTRNGKMNAAAMVRTSVEMHEEGLLTREDAITRVNPAHLEQMLFPR
                     LDPRHRGQALATGLPASPGAASGQAVFDADRAEMLGRRGARVILVREETKPEDIHGFF
                     ASQGILTSRGGKTSHAAVVARGMGKPCVAGAEGIQVDVAMRVAFVGDQVIKEGDVITI
                     DGSSGQVFRGEVPTVEADFSPSLVKLLGWADAKARLGVRANADTPDDAARALRYGAQG
                     IGLCRTERMFNAADRLPIVVDMIVADTTDERRAALDRLLPIQRADFIGIFKAMTPHPV
                     TIRLLDPPIHEFLPTERQLEDDIARLRDLRAVTRGMEVLGEATHDEADARLVSLLRDL
                     GDTRIVDAVLTRKEAVLRKARALREVNPMLGHRGVRLGITYPEIYTMQVRAVLEAAAD
                     CIKAGIEVHPEIMVPQVCTAQELKLVREYMTAVQTQVEVTYGVKVPCRFGTMIEVVRA
                     CMRAEHLAEVAEFFSFGTNDLTQATFSFSREDAENKFLPMYNERGILQDNPFEVLDVK
                     GVGRLMALAVDWGRATRAGLKVGICGEHGGHPASIGFCNSIGLDYVSCSAPRVPIARL
                     AAAHAAISGAAPLDKAA"
BASE COUNT          367 a          877 c          889 g          417 t
ORIGIN      
        1 ggcgggctgc gggtcgacat cacgctcccg cgctgacggg aaaatgggga cattctccta
       61 tttcgcgacc gcgaaatagg agaatgtccc cactttcccg tcaggctgcc ttgtcgagcg
      121 gtgcggcacc gctgatcgcg gcgtgggcgg cggccagccg cgcgatcggc acgcgcggcg
      181 ccgagcacga cacgtagtcg aggccgatcg agttgcagaa gccgatcgac gccgggtgac
      241 cgccgtgctc gccgcagatg ccgaccttca gcccggcgcg ggtcgcccga ccccagtcca
      301 cggcgagggc catcagacgg ccgacaccct tcacgtccag cacctcgaac gggttgtcct
      361 gcaggatccc acgctcgttg tacatgggca ggaacttgtt ctcggcgtcc tcgcgggaga
      421 acgagaacgt ggcctgcgtc aggtcgttgg tgccgaacga gaagaattcc gccacctcgg
      481 cgagatgctc ggcgcgcatg caggcgcgca ccacctcgat catggtgccg aaccggcacg
      541 ggaccttgac gccgtaggtc acttcgacct gggtctggac ggctgtcatg tactcgcgca
      601 cgagcttgag ctcctgtgcg gtgcagacct gcggcaccat gatttcgggg tgcacctcga
      661 tccctgcctt gatgcagtcg gccgcggcct ccagcacggc ccggacctgc atcgtgtaga
      721 tctcggggta ggtgatgccg agccgcacgc cacgatggcc gagcatcggg ttcacctcgc
      781 gcagcgcgcg ggccttgcgc agcaccgctt ccttgcgcgt cagcacggcg tcgacgatcc
      841 gtgtgtcgcc gaggtcacgc agcagcgaca cgaggcgcgc gtcggcctcg tcgtgggtcg
      901 cctcgccgag cacctccatg ccgcgtgtca cggcgcgcag gtcgcgcagc cgcgcgatgt
      961 cgtcctcgag ctgtcgctcg gtcgggagga actcgtggat cggcgggtcg agcaggcgga
     1021 tcgtcaccgg gtgcggcgtc atggccttga agatgccgat gaagtcggcg cgttgtatgg
     1081 gcagcaaccg gtccagggcg gcacgccgct cgtcggtcgt gtcggccacg atcatgtcga
     1141 cgacgatggg cagccggtcg gccgcattga acatccgctc ggtgcggcac agcccaatgc
     1201 cctgcgcgcc gtagcgcagc gcgcgagcgg cgtcgtccgg cgtgtcggcg ttcgcgcgca
     1261 cgccgagccg cgccttggcg tcggcccagc ccagcagctt cacgagcgac ggcgagaagt
     1321 cggcctccac ggtcggcacc tcgccgcgga agacctggcc gctgctgccg tcgatcgtga
     1381 tcacgtcacc ttccttgatg acctggtcgc cgacgaacgc gacccgcatc gcgacgtcca
     1441 cttggatgcc ttcggctccg gcgacgcaag gcttgcccat gccacgggcg acgacagccg
     1501 cgtgcgaagt cttgccgccg cggctggtga ggatgccctg cgaggcaaag aagccgtgga
     1561 tgtcctccgg cttggtctcc tcgcggacca ggatgacccg ggccccgcgc cggccgagca
     1621 tctccgcgcg gtcggcgtcg aacacggcct gaccgctcgc cgcgcccggg gaggcgggca
     1681 gcccggtcgc gagcgcctgt cccctgtgcc gcgggtcgag gcgcgggaag agcatctgct
     1741 cgaggtgcgc cgggttgacc cgcgtgatgg cgtcctcgcg ggtgagcagt ccttcctcgt
     1801 gcatctcgac cgacgtgcgc accatcgccg ccgcgttcat cttgccattg cgggtctgca
     1861 ggcagtagag cgtgccgcgc tcgacggtga actcgaagtc ctgaacttcg cgatagtgcc
     1921 tctcgagccg gttgcgcagc tcgacgagct gcgcgtggat ggccggcatc tcgccggcca
     1981 tctcggcgat cggccggggc gtgcggatcc cggccaccac gtcctcgccc tgcgcgttcg
     2041 tgaggtactc gccgtagagc tggttctcgc cggtcgcagg attgcgcgtg aagccgacgc
     2101 cggtcgcgct gtcgttgccc atgttgccga acaccatggt gacgacgttc acggccgtgc
     2161 cgttcgccat cgcgggcgtg atgcggaact cgcgccggta gtcgatggcg cgcttgccca
     2221 tccacgaatt gaagacggcc ttgatcgcga tctcgagctg ctcgtacgga tccgacggga
     2281 acgggcgccc cgtgtgccgc tgtacgaccg cgaggaagcg gtcgccgacg tcgcgcagct
     2341 cgtcggggcc cagcatgacg tcgtgcttca cgccggcccg gctcttcacg gcctcgaact
     2401 gctcgtcgaa cagcgcgtcc gggacgccga gcgccacctt gccgaacagc tgcacgaagc
     2461 ggcgccaggc gtcgtagccg aagcgctcgt tgccggtgcg gcggatgagc gcggcgagcg
     2521 tggtctcgtt gagcccgagg ttgaggatcg
//
LOCUS       DAOMFD010000265         2538 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_22726_length_2538_cov_33.726138, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000265 DAOMFD010000000
VERSION     DAOMFD010000265.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2538)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2538
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22726_length_2538_cov_33.726138"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1137)
                     /gene="eno"
                     /locus_tag="PL152_10735"
     CDS             complement(<1..1137)
                     /gene="eno"
                     /locus_tag="PL152_10735"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     2-phospho-D-glycerate in glycolysis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HQR49803.1"
                     /translation="MSATIRSIHARRVWDSRGRPTVEAEVTLNDGAIGRAIVPAGASK
                     GTREALELRDGSARFGGLDVLRAVGNVNGEIAQALVGHAADDQAALDARLIALDGTET
                     KARLGANATLAVSMAAAQAAAAAQGVPLYEYLGGADATLLPMPQIQIFGGGAHAGRRV
                     DVQDFMIVCPGATSFAGALEMTAEVYRCAGELLQSRGARYGVADEGGWWPAFSTNAEA
                     LETLVAAIERAGFVPGEEVAIALDIAASDFGRAGRYRLGLEGRELDSDGLIELLLGWI
                     ERYPIVSIEDPLGEDDPDGFARFTQAAGHRLQIVGDDFLVSHAPLIERAAAIGAANTV
                     LLKPNQRGTLTETLQAWRAARTAGYAGIVSARSGETEDTTIVHLA"
     gene            complement(1134..2150)
                     /locus_tag="PL152_10740"
     CDS             complement(1134..2150)
                     /locus_tag="PL152_10740"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HQR49804.1"
                     /translation="MGAATEVEVPAQFAQALRELGLAGKDAIFGTPLTGGVSSDIWLI
                     QTARGPVCAKRALAKLRVAADWRAPIERNRYEARWMRVACAAHEGCAPRVLGQHPALG
                     VLVMDYLPPADFALWKQRLRDGQADVSTAQEVGRVLGAIHAYSARHPELAAEFDTDTI
                     FFDIRLEPYLLATARKHPDLAARLEQLVDVTRSQRRALVHGDVSPKNILIGANGPVFL
                     DAECAWWGDPAFDIAFCLNHLLLKCLWTPAATPAFLASFDAMADAYLSAVEWEPSGAL
                     EQRAAALLPGLFLARVDGKSPVEYITDEGQREQVRLVARELLAHPVGSLRSVRARWAE
                     ELGQ"
     gene            complement(2134..>2538)
                     /locus_tag="PL152_10745"
     CDS             complement(2134..>2538)
                     /locus_tag="PL152_10745"
                     /inference="COORDINATES: protein motif:HMM:NF020135.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketopantoate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HQR49805.1"
                     /translation="SNDVMAELWSKLMVNCAYNAISALAQSPYASLAAQPEIRDIQHA
                     IVREVVQLAQAEGIGLQLEPSLQAMERIAIAMPAQFSSTAQDMARRKPSEIDHLNGFI
                     VRRGRELGVAVPVNQTLTALVKLVEAGYGRGD"
BASE COUNT          388 a          893 c          862 g          395 t
ORIGIN      
        1 ggcgagatgc acgatggtcg tgtcctccgt ctcgcccgag cgcgccgaga cgatgcccgc
       61 gtagcctgca gtacgcgcgg ccctccacgc ctgcagcgtt tccgtcagcg tgccgcgctg
      121 gttgggcttg agcaggaccg tgttggcggc cccgattgcg gccgcccgct cgatcagcgg
      181 tgcgtgcgag accaggaagt cgtcgccgac gatctgcagg cgatgtcccg ctgcctgcgt
      241 gaagcgcgca aacccgtcgg gatcgtcttc gcccagcggg tcctcgatcg agacgatggg
      301 atagcgctcg atccacccca gcagcagttc gatcagtccg tccgaatcca gctcgcgtcc
      361 ttccagtccg agccggtagc gcccggcccg gccgaagtcc gaggcggcga tgtccagtgc
      421 gatggccact tcctcacccg gcacgaagcc ggcgcgctcg atggcggcga cgagcgtctc
      481 gagcgcctct gcattcgtcg agaacgccgg ccaccatccg ccttcgtcag caacgccgta
      541 gcgcgcacca cgtgactgca gcagctcgcc ggcgcagcga tacacctctg cggtcatctc
      601 aagcgccccg gcaaagctgg tggcaccggg gcagacgatc atgaagtcct gcacgtcgac
      661 acggcggccc gcgtgcgcgc cgccgccaaa gatctggatc tgcggcatcg gcagcagcgt
      721 ggcatcggct cctccgaggt attcgtagag gggcaccccc tgcgctgctg ccgccgcctg
      781 cgccgcggcc atcgaaaccg ccagcgtcgc gttcgccccg aggcgagcct tcgtttcggt
      841 gccgtcgagc gcgatcagcc gggcgtcgag cgcggcctgg tcgtcggcgg cgtggccgac
      901 gagcgcttgc gcgatctcgc cgttgacatt gccgaccgcc cgcagcacgt caagtccgcc
      961 gaaccgcgcc gacccatcgc gcagctcgag cgcttcgcgc gtgcctttcg aggcgccggc
     1021 cggaacgatc gctcggccga tcgctccgtc gttcagcgtc acctccgcct cgaccgtggg
     1081 ccggccgcgg ctgtcccaga cgcgccgcgc gtggatcgag cggatcgtcg cgctcactgt
     1141 ccgagttcct ccgcccaacg cgcgcgaacc gagcgcaggc tgccgaccgg gtgcgccagc
     1201 agttcacgtg ccactagccg cacctgctca cgctgccctt cgtccgtgat gtattcgacc
     1261 ggggacttgc cgtcgacccg tgcaagaaag agccccggca acagggccgc ggcgcgctgt
     1321 tcgagtgcgc ccgatggctc ccattccacg gcacttaggt aggcgtccgc catcgcgtcg
     1381 aacgaagcga ggaaagccgg cgtcgcagcg ggcgtccaca ggcacttgag cagcaggtgg
     1441 ttcaggcaga aggcgatgtc gaacgcggga tcgccccacc aggcgcactc ggcgtcgagg
     1501 aaaacaggtc cgttcgcccc gatgaggatg ttcttcgggc tgacgtcgcc gtggaccagc
     1561 gcccggcgct gggagcgcgt gacgtcgacg agctgctcga gacgggccgc gaggtcagga
     1621 tgcttccgcg cggtggcgag caggtacggc tcgaggcgga tgtcgaagaa gatcgtgtcg
     1681 gtgtcgaact ccgcggcgag ctccggatgc cgcgccgagt aggcgtggat tgctccgagc
     1741 accctcccca cttcctgcgc ggtagacacg tcggcctgcc cgtcgcgcag tctctgcttc
     1801 cagagggcga agtcggcggg cggcaggtag tccatgacca ggacgccgag cgccggatgc
     1861 tggcccagga cacgcggcgc gcacccctcg tgcgcggcgc aggccacacg catccagcgt
     1921 gcttcgtaac ggttgcgctc gatcggggca cgccagtcgg ccgccacacg cagtttcgcc
     1981 agcgcgcgct tggcgcacac aggcccccgg gcggtctgga tgagccagat gtcggaggag
     2041 acccctccgg tcagcggtgt gccgaagatc gcgtccttgc cggcgaggcc gagctcacgg
     2101 agggcctgcg caaactgggc gggaacctcg acttcagtcg ccgcgcccat atccggcttc
     2161 gacgagcttg accagcgcgg tcagtgtctg gttgacggga acggccacgc ccagttcgcg
     2221 gccgcggcgg acgatgaacc cgttcagatg atcgatctcc gaaggcttcc tgcgcgccat
     2281 gtcctgcgcg gtcgaggaaa actgcgcggg catcgcgatc gcgatccgtt ccatcgcctg
     2341 cagcgacggc tccagttgca ggccgatgcc ctcggcctgc gcgagctgaa cgacttcgcg
     2401 cacgatggcg tgctggatgt cgcggatttc cggctgcgcc gcgaggctgg catacggcga
     2461 ctgagcgagt gccgagattg cgttgtaggc gcaattcacc atcagcttcg accagagctc
     2521 ggccatgacg tcgttcga
//
LOCUS       DAOMFD010000266         2498 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_23345_length_2498_cov_28.657798, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000266 DAOMFD010000000
VERSION     DAOMFD010000266.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23345_length_2498_cov_28.657798"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..444)
                     /locus_tag="PL152_10750"
     CDS             complement(<1..444)
                     /locus_tag="PL152_10750"
                     /inference="COORDINATES: protein motif:HMM:NF014898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HQR49806.1"
                     /translation="MSTAVKTSALARPQRDSRASHGPGPIDRFLDAVWMERGLAPNTL
                     AAYRADLTALDRWLENHETELTSAKRGDLLEFMAARAQAGARPRSTARQLSSFRRFYR
                     YLVREGSLREDPTAQIAMPKVGRSLPRSLTEEEVEALLAAPTVSDP"
     gene            605..1381
                     /locus_tag="PL152_10755"
     CDS             605..1381
                     /locus_tag="PL152_10755"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HQR49807.1"
                     /translation="MLRVMGRALGSLYGLCALAIFCLMFALAALLAVIVPRLRWRRTL
                     THQLARVAVVLFGLRLSTTGLERLPSGSCVVVANHESYLDGVLLKAVLPPRFSFVIKR
                     EAASLPVAGFLLRRIGSVFVDRNTRGGRQRDARRVLEHAELGHSLVFFPEGTFDETAG
                     LKRFHVGAFTAAVRGGMPLVPVVIRGARRAMPPGAYFPRAGRIELEVLDPLPADGPDV
                     SADRLRDEARRRMLARLDLPDLAAGPPSPHADVDAGRNPA"
     gene            complement(1390..>2498)
                     /locus_tag="PL152_10760"
     CDS             complement(1390..>2498)
                     /locus_tag="PL152_10760"
                     /inference="COORDINATES: protein motif:HMM:NF018452.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HQR49808.1"
                     /translation="YSIAWLDVTREPIVLSVPAIEDRYYSFCLTGYDADNFAYVGTRA
                     TGTGAGDYAIVGPHWKGTLPKGVRALTKAPTPVVFVLGRTMVKGPDDLEAVHRLQSRY
                     RLTPLSQWGRPDAKRSDDRNVWAPYSAKDDPLADWKTINRAMTEIPPPPGNETLLELF
                     ARIGIGPGQDVDRTDEATQRGLVRALETGKRVVTGAATFMAGSTVVDGWRYTPDYYGR
                     DGLHKDFLGRAGPVSLAGIVANDPVEAMYPSTRVDSSGEPLSGAHKYTLHFGKGQLPP
                     VTAFWSLTAYGMDFNLIANPIDRYSLGDRSALKTDADGGVTVYLQADSPGKELESNWL
                     PVGGQGFYLVARLYLPKPEATAGQWHLPPVRRTD"
BASE COUNT          349 a          849 c          866 g          434 t
ORIGIN      
        1 cgggtcgctc acggtcggag cggccagcag ggcctcgact tcctcctccg tgagcgaccg
       61 cggtagcgag cggccgacct tgggcatggc gatctgggcg gtcgggtcct cgcgcagcga
      121 gccctcgcgc accaggtaac ggtagaaacg gcggaagctc gacagctggc gcgccgtcga
      181 acgcggccgc gcgccagcct gggcgcgcgc tgccatgaat tcgaggaggt ccccgcgctt
      241 ggcggaggtg agttccgtct cgtggttctc gagccagcgg tcgagcgccg tgaggtcggc
      301 gcggtaggcc gcgagcgtgt tcggcgcaag cccccgctcc atccagaccg cgtcgaggaa
      361 gcggtcgatg ggaccgggcc cgtgcgaggc gcggctgtcg cgctggggcc gggcgagagc
      421 gctggtcttg actgctgtgc tcatgggatc tcggaaccgt tcgtggcgcg gagtatgcga
      481 gccgcccgtg cccgatcgtc gtgacacccc tcacagattg gtcccaccat taacataact
      541 ggatgcagtt cacagaatgt gtccgggcgt gcagactgcc cgcgccgcaa gagggtgcca
      601 cgcaatgttg cgagtgatgg gccgggcgct ggggagtctg tacgggctct gcgcgctggc
      661 cattttctgc ctgatgttcg cgctggccgc cctcctcgcc gtgatcgttc cgcgactccg
      721 ttggcggcgg accctgacac accagctcgc gcgcgtcgcc gtcgtactgt tcggcctgcg
      781 gctctcgacg acggggctgg aacgactgcc gtccgggtcc tgtgtcgtcg tcgcgaacca
      841 cgagagctat ctcgacggtg tcctgctcaa ggcggtgctg ccgccccgct tctcgttcgt
      901 gatcaagcgc gaggccgcga gcctgccggt ggcaggattc ctgctccggc gcatcggctc
      961 cgtcttcgtc gaccgcaata cccgcggcgg ccggcaacgc gatgcgcggc gcgtgctcga
     1021 gcatgccgag ctgggtcatt cgctggtgtt cttcccggaa ggcacgttcg acgagacggc
     1081 cggactcaag cgcttccacg tcggggcatt caccgcggcc gtgcgtggcg ggatgccgct
     1141 ggttccggtc gtcattcgcg gcgcgcggcg cgcgatgcca ccgggggcgt actttccaag
     1201 ggccggacgc atcgaactgg aggtcctcga cccgctgcca gccgatgggc cggacgtgtc
     1261 ggccgaccgg ctgcgcgacg aggcgcggcg acgcatgctg gctcgcctcg acctgccgga
     1321 tcttgccgcc ggcccgccgt cgccgcatgc cgacgtcgac gccggtcgga accctgcctg
     1381 aacgagcgtt cagtctgtcc ggcgcaccgg cggcaggtgc cactggcctg ccgttgcttc
     1441 cggtttaggc aggtacaacc gcgcgaccag gtagaaacct tggccgccga cgggcagcca
     1501 attggactcc aattccttgc ccggcgaatc cgcctgcagg tagacggtca caccgccgtc
     1561 cgcgtccgtc ttcagggcgc tgcgatcgcc aagcgagtag cggtcgatgg ggttcgcgat
     1621 caggttgaag tccatgccgt acgcggtgag cgaccagaac gcagtcaccg gcggcaactg
     1681 gcccttcccg aaatgcagcg tgtacttgtg cgcgccgctg agcggttctc ccgacgaatc
     1741 gacgcgcgtg ctcgggtaca tggcctccac cgggtcgttc gccacgatgc ccgcgagact
     1801 gacgggaccg gcgcgcccca ggaaatcctt gtgcagcccg tcgcggccgt agtagtcggg
     1861 cgtatagcgc cagccgtcca cgaccgtgga acccgccatg aaggtggccg caccggtgac
     1921 gacgcgcttg ccggtctcca gcgcccggac cagtccgcgc tgcgtcgctt cgtccgtcct
     1981 gtcgacgtcc tggccgggac cgatgccgat gcgtgcgaac agttccagca gtgtctcgtt
     2041 gccgggcggt ggcgggatct cggtcattgc ccggttgatg gtcttccagt ccgcgagtgg
     2101 atcgtccttc gcgctgtagg gtgcccagac attgcgatca tcggaccgct tggcgtccgg
     2161 ccggccccat tggctgagcg gcgtgagcct gtaccgggac tgcaggcgat gcacggcctc
     2221 gaggtcgtcc ggccccttga ccatcgtgcg cccgagcacg aatacaaccg gcgttggggc
     2281 tttcgtcagc gctcgcacgc ccttgggcag ggttcccttc cagtgcggcc cgacgatcgc
     2341 gtagtcgccg gcccccgtac cggtcgcgcg cgtgccgacg tatgcgaagt tgtcggcgtc
     2401 gtagcccgtc aggcagaacg agtaataccg gtcctcgatt gccggcaccg agagaacgat
     2461 cggttccctg gtcacgtcca gccacgcgat cgaataca
//
LOCUS       DAOMFD010000267         2488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_23504_length_2488_cov_26.157419, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000267 DAOMFD010000000
VERSION     DAOMFD010000267.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23504_length_2488_cov_26.157419"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="PL152_10765"
     CDS             complement(<1..642)
                     /locus_tag="PL152_10765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003090278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose 5-phosphate 3-epimerase"
                     /protein_id="HQR49809.1"
                     /translation="MNHELPWVAERVRLFRESDADFARWARGYGPIVHNEVTQLRVHE
                     LAQVLVAGGKASDTASVFRTLWAADRLAAAGMWLTVHMTYADRVHPDGREMAAEDFKH
                     TPEGHTGGSLNIVPAYVGYLAANALSGLTRGWLMGQGHCVAGIDACNLLVGNMTPRHA
                     ERYDRSESGLTRFARDFYSYAIDATGRPASPLGSHVGPNTAGGLSEGGYLGFAE"
     gene            complement(639..1466)
                     /locus_tag="PL152_10770"
     CDS             complement(639..1466)
                     /locus_tag="PL152_10770"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HQR49810.1"
                     /translation="MSALLVARRTLLRTLAVGGMSTAVAPLGRSQVSEGREMLDKVEK
                     PLWGSTVQGEYETTITTPRRQPTLGLRLSMDRPRRSFVRILSSAKEAGIGSLRIGTEM
                     WNYLPNVERIVKIPPSMMLQPWMGSDVTNDDLVKESSILDDYTHKVLATVQLDGEAVV
                     QVEAVPKPDAAVVWGRIVYWVRRTDTMPLKQEFFSERGERVRVLAFSDVRKVGGRVLP
                     TRWDIRLDAKPGNSTTVVFKDAVFDRPVDDEIFSQRHLQRRWAPTPRAVFGATRCRR"
     gene            complement(1463..>2488)
                     /locus_tag="PL152_10775"
     CDS             complement(1463..>2488)
                     /locus_tag="PL152_10775"
                     /inference="COORDINATES: protein motif:HMM:NF013593.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR49811.1"
                     /translation="EQTLNQLLAEMDGFDTNRGVIIMAATNRPEILDPALLRPGRFDR
                     HVAIDRPDLNGRRQILEVHVKHVRLAANVDLAVVAARTPGFAGADLANLVNEATLRAA
                     KHDKPAVEMSDFEEALDRIVAGLEKRNRVMNPMERRFVAFHEAGHAVVTEMRKNTDRV
                     SKVSIIPRGIAALGYTQQSPTEDRYLMRKSELLDRIDVLLGGRVAEKLVFDDVSTGAE
                     NDLQRATDMARHMITHYGMSESLGLVTFDTRPVPLYLNAPTIPQPRTFSERTAEAIDG
                     EVRRILDEARERVTETLTAHRATLDTLAALLLEKEVVDRSMLDRLMSEQAAPAQLRVA
                     AVPVRPE"
BASE COUNT          357 a          870 c          845 g          416 t
ORIGIN      
        1 ctccgcgaag ccgaggtaac cgccctcgga caggccaccg gccgtgttcg gcccgacgtg
       61 gctgcccagc ggcgaggccg gccgccccgt cgcgtcgatc gcgtagctgt agaagtcgcg
      121 ggcaaagcgc gtcaacccgc tctcgctgcg gtcgtagcgc tcggcgtggc gaggcgtcat
      181 gttgcccacc agcaggttgc aggcgtcgat gcccgccacg caatgcccct gccccatcag
      241 ccagccgcgc gtcaggccgc tgagcgcgtt cgcggcaaga tagccgacgt atgccggcac
      301 gatgttcagc gagccgccgg tatggccctc gggggtgtgc ttaaagtcct cggccgccat
      361 ctcgcgcccg tcgggatgaa ccctgtcggc gtaggtcatg tgcacggtca accacatgcc
      421 ggccgcggcg aggcggtctg cggcccacag cgtgcggaag acgctcgccg tgtcgcttgc
      481 cttgcccccg gcgaccagca cctgcgcgag ctcgtgcacg cgcagctgag tcacttcgtt
      541 gtgcacgatg ggcccgtagc cgcgcgccca gcgcgcgaag tcggcatcgg actcgcggaa
      601 cagccgcacg cgctcggcga cccacggaag ctcatggttc atcgtctgca ccttgttgcg
      661 ccgaacacag cgcgcggggt tggggcccag cgcctctgca ggtggcgctg gctgaagatc
      721 tcgtcgtcca ccggccggtc gaacacggcg tctttaaaca ccaccgtggt cgagttgccg
      781 ggcttggcgt cgagccgtat gtcccagcgg gtcggcagga cccgcccgcc gaccttgcgc
      841 acatcggaga acgccagcac gcgcacgcgc tcgccgcgct cgctgaagaa ctcctgcttc
      901 aacggcatcg tgtcggtgcg ccgcacccag tagacgatgc gcccccacac caccgccgcg
      961 tcgggtttcg gcacggcctc gacctggacg accgcctcgc cgtcgagctg cacggtggcg
     1021 agcaccttgt gcgtgtagtc gtcgaggatg ctgctctcct tgacgaggtc gtcgttcgtg
     1081 acgtccgagc ccatccacgg ctgcagcatc atcgacggcg gaatcttgac gatgcgctcg
     1141 acgttgggca ggtaattcca catctcggtg ccgatgcgca gcgagccgat gccggcctcc
     1201 ttggctgacg acaggatgcg cacgaaggag cgccgcggcc ggtccattga caggcgcagg
     1261 ccgagcgtgg gctgccggcg cggcgtggtg atcgtcgtct cgtactcgcc ctgcaccgtg
     1321 ctgccccaca gcggcttctc gaccttgtcg agcatctcgc ggccctcgga cacctgtgac
     1381 cgacccagcg gcgcgacggc cgtggacatg ccgcccacgg ccagcgtgcg cagaagagtg
     1441 cggcgcgcga cgagcaacgc actcattcgg gccgcaccgg cacggcggcc acgcgcagct
     1501 gcgcgggcgc cgcctgctcg ctcatcaggc ggtcgagcat cgaccggtcg acgacttcct
     1561 tctccagcag cagcgccgcc aacgtgtcga gggtggcccg gtgcgctgtc agcgtctcgg
     1621 tcacgcgctc gcgcgcctcg tcgaggattc gtcgcacctc gccgtcgatc gcctccgcgg
     1681 ttcgctcgct gaaggtgcgc ggctggggga tcgtcggggc gttcaggtac agcggcacgg
     1741 ggcgtgtgtc gaaggtgacc agcccgagcg attcgctcat gccgtagtgc gtgatcatgt
     1801 gccgcgccat gtcggtggcg cgctgcaggt cgttctccgc gccggtcgac acatcgtcga
     1861 agacgagctt ctcggccacg cggccgccga gcagcacgtc aatgcggtcg agcagctcgc
     1921 tcttgcgcat caggtagcga tcctcggtcg gcgactgctg cgtgtacccg agcgccgcta
     1981 tgccacgcgg gatgatcgag accttggaaa cgcggtcggt gttcttgcgc atctcggtca
     2041 ccaccgcgtg cccggcctca tggaaggcga cgaacctgcg ctccatcggg ttcatcaccc
     2101 ggttcctctt ttcgaggccg gcgacgatcc ggtcgagcgc ctcctcgaag tcgctcatct
     2161 cgacggccgg tttgtcgtgc ttcgccgcgc gcagcgtcgc ttcgttgacc aggttggcga
     2221 ggtcggcgcc ggcgaagccg ggagtgcggg ccgccaccac cgccaggtcg acattggcgg
     2281 ccagcctgac gtgtttgaca tgcacttcga ggatctggcg gcgaccattc aggtcgggac
     2341 ggtcgatcgc gacgtgacga tcgaagcggc cagggcgcag cagcgccggg tcgaggatct
     2401 ccggccggtt cgtcgcagcc atgatgatca cgccgcggtt ggtgtcgaag ccgtccatct
     2461 cggcgagcag ctggttcagc gtctgctc
//
LOCUS       DAOMFD010000268         2481 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_23610_length_2481_cov_31.950536, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000268 DAOMFD010000000
VERSION     DAOMFD010000268.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2481)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23610_length_2481_cov_31.950536"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /locus_tag="PL152_10780"
     CDS             complement(<1..402)
                     /locus_tag="PL152_10780"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Members of this family of hydrolases with an active
                     site Cys residue belong to MEROPS family C26; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HQR49812.1"
                     /translation="MAERKPLIGIPADRRILGPHPFHCVGEKYIRAVAEAADAVPVLI
                     PALGDEVDFDAVLGAVDGILLTGSPSNVEPTRYAGPVSEPGTMHDPHRDATTLPMIPR
                     VVESGMPLFAICRGFQEMNVAYGGTLWQKVQE"
     gene            641..2011
                     /locus_tag="PL152_10785"
     CDS             641..2011
                     /locus_tag="PL152_10785"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HQR49813.1"
                     /translation="MPQFADFEQARAWLAGHRSVRAVDLLLPDLMGIPRGKRVTVAEL
                     ESVHRNGLLLPASMFALDVLGGTVQETGLGFDEGDADRVCLPVPGSLATVPWLGEHVA
                     QMQVAMYEHDRAPFYGDPRHALERVVGRLAELGLRPVMAIELEFYLVDRERTAAGCAQ
                     PPRQPLTGRREHKTQINSMADLDEYSAIIAAFDEAARAQELPTGTVLAEYGPGQFEVN
                     LHHVDDALLACDHAIRLKRLVKGVALQHGMEATFMPKPYRDHAGSGTHVHVSLVDAQG
                     RNIFGAEDPAGSPQLRHAIGGLAATINDMMAVCAPTANSYRRFQPEAYVPLNPSWSVN
                     NRGVAFRVPHGPPASRRVEHRVAGADANPYLLAAMVLAGMHLGLTQKLDPGPVLAGNA
                     YRDTTPTIPLTWPEALGAFERSAFARDYLGERFARLYAQTRRGEMQDFGSYVSPLEYS
                     WYLTTS"
     gene            2015..>2481
                     /locus_tag="PL152_10790"
     CDS             2015..>2481
                     /locus_tag="PL152_10790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HQR49814.1"
                     /translation="MEHVRSYYAATAHASPLREPLRGEVTADVCVVGGGIAGCSTALH
                     LAERGYKVVLLEGQRIGWGASGRSGGQAIFGFAAGQDKLVRQVGQETARRMFDVSVDA
                     LDLLKERVARHAIDCDLHWGQAHVAIKPRHETELKAWREELERDYGYTSLRWL"
BASE COUNT          406 a          877 c          838 g          360 t
ORIGIN      
        1 ctcctgcacc ttctgccaga gcgtgccgcc gtaggcgacg ttcatctcct ggaagccgcg
       61 gcagatcgcg aagagcggca tgcccgactc gacgacccgc gggatcatcg gcaacgtggt
      121 cgcgtcgcgg tgcgggtcgt gcatcgtgcc cggctcactg acagggccgg cgtagcgcgt
      181 cggttccacg ttcgacgggc tgccggtcag cagtatcccg tccaccgcac cgagcactgc
      241 atcgaagtcc acctcgtcgc cgagggccgg aatcagcacc ggcaccgcgt ccgcggcctc
      301 ggccacggcg cggatgtatt tctccccgac gcaatggaag gggtgcggcc cgaggatccg
      361 gcgatccgcg ggaatgccga tcagtggttt gcgctcagcc acgtttaaga tcctagacgg
      421 cctatgagcg gccgccggac gagtgcagac cggattaaac cacagggacg gggcaggcgt
      481 cgccgagcag tgcaaaaaaa cggccaaaaa tcagcccgaa cagcgagttg cgcgcccttc
      541 ccagccgcgc ccggcgcttg ccgccgcggg ccccgtgcag ctatgctcga cgatcaagcg
      601 cggcccgtcc gtcgctaaat cctaaacaac cgggggtccc atgccgcagt tcgcggattt
      661 cgagcaggcg cgtgcgtggc tcgccgggca ccgcagtgtc cgggccgtgg acctgctgct
      721 gccggatctc atgggcattc cgcgcggcaa gcgggtgacg gtcgcggagc tcgagagcgt
      781 ccaccgcaac ggcctgctgc tgcccgcatc catgttcgca ctcgacgtgc ttggagggac
      841 cgtccaggaa acggggctgg gcttcgacga gggcgacgcc gaccgggtct gcctgcccgt
      901 gcccggatcg ctcgccacgg taccctggct gggcgaacac gtggcgcaga tgcaggtcgc
      961 gatgtatgaa cacgaccgcg cgccgtttta cggcgacccg cggcatgcac tggaacgcgt
     1021 cgtgggcagg ctggcggaac tcggcctgcg accggtcatg gccatcgagc tggagtttta
     1081 tctcgtcgac agggaaagga ctgcggctgg ttgcgcgcag ccgccgcgcc agccgttgac
     1141 cggccgtcgc gagcacaaga cgcagatcaa ctcgatggcg gacctcgacg agtactccgc
     1201 gatcatcgcg gcattcgacg aggccgcgcg ggcacaggaa ctccccacgg gcaccgtgct
     1261 cgccgaatac ggcccgggcc agttcgaggt gaacctgcac cacgtcgacg acgccctgct
     1321 cgcctgcgac cacgccattc gcctcaagcg cctcgtgaag ggcgtcgcgc tccagcacgg
     1381 catggaggcg acgttcatgc ccaagcccta tcgcgaccat gcgggcagcg gcacgcacgt
     1441 gcacgtgagc ctggtggacg cacagggacg caacatcttc ggggccgagg accctgccgg
     1501 cagcccgcag cttcgacacg cgatcggcgg attggcggcc acgatcaacg acatgatggc
     1561 ggtgtgcgct ccgaccgcga actcgtatcg acgcttccag cccgaggcct acgtgccgct
     1621 caacccgagc tggtccgtga acaaccgcgg cgtcgcgttc cgcgtgccgc acgggccgcc
     1681 ggcgagccgc cgggtcgagc accgcgtcgc cggcgcggac gcgaatccct acctgctcgc
     1741 ggcgatggtc ctggccggaa tgcacctcgg cctcacgcag aaactcgacc ccggccccgt
     1801 gctcgccggc aacgcgtatc gcgacaccac gcccaccatc ccgctcacct ggcccgaggc
     1861 cctgggcgcc ttcgagcgca gtgccttcgc gcgcgactac cttggggagc gcttcgcgcg
     1921 gctctacgcc cagacgcgcc gtggcgagat gcaggacttc ggctcctacg tttcgccact
     1981 cgaatattcc tggtacctga cgaccagctg aaccatggaa cacgtccgct cgtactacgc
     2041 cgccacggcc cacgcctcgc cactgcgcga gcctttgcgc ggcgaggtca cggcggacgt
     2101 gtgcgtggtc ggcggcggca tcgccggttg ctcgaccgca ctgcacctcg cagagcgcgg
     2161 ctacaaggtc gtgctgctgg agggccagcg gatcggctgg ggcgcgtcgg ggcgcagcgg
     2221 cgggcaggcg atcttcgggt tcgcggccgg gcaggacaag ctcgtccggc aggtcgggca
     2281 ggagaccgcg cgccgcatgt tcgacgtttc ggtcgatgcg ctcgacctgc tgaaagagcg
     2341 cgtcgcgcgc cacgcgatcg actgcgacct ccactgggga caggcgcacg tcgcgatcaa
     2401 gccgcggcac gagaccgaac tcaaggcctg gcgcgaggaa ctcgagcgcg actacggcta
     2461 cacctcgctg cgctggctcg a
//
LOCUS       DAOMFD010000269         2479 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_23641_length_2479_cov_28.442244, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000269 DAOMFD010000000
VERSION     DAOMFD010000269.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2479)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2479
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23641_length_2479_cov_28.442244"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(38..733)
                     /locus_tag="PL152_10795"
     CDS             complement(38..733)
                     /locus_tag="PL152_10795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49815.1"
                     /translation="MLQPRHARLTRTAQRSVTRRRSVVHTWAFALGLVVLAGSAMQTH
                     EAAAAGASLPFARVLVVGVSPNEDRRCQFEQFLASQITGKETQAYASCDVLDQINPLT
                     RESIDKAVAAVQADGVLATVLVSKQQQVEQGDDRDTRGGAQYKEAGLGFAPDYWGVWG
                     VPVVYTDFEQWPPVNLLEGEVHVATRVFDASSATLLDTVDTTVKHIEETDVGMDKVAE
                     SIAKDLHRHGYIH"
     gene            997..1082
                     /locus_tag="PL152_10800"
     tRNA            997..1082
                     /locus_tag="PL152_10800"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Leu,seq:tag)
     gene            1184..2479
                     /gene="tig"
                     /locus_tag="PL152_10805"
     CDS             1184..2479
                     /gene="tig"
                     /locus_tag="PL152_10805"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HQR49816.1"
                     /translation="MQVSVEQTGALERRMEVSVPKERVEQAVHKRLQQVGRTAKLKGF
                     RPGKAPLKVIQQQFGAQVRQEVVGDLMQSSFAEAITQHNLSPAAGPRIEPITMTPGED
                     LKYRAIFEVYPEVALASVEGLDVIRPVAEVTEGDIDAMVENLRSQRPKFETVERESRE
                     GDRVTMDFVGLLDGAEFEGSKGTDVAVLLGSGRMIKEFEAGITGMTAGEQRKIDVRYP
                     DDYHNKSLAGRTANFDVHMKKVEEKRLPDLDDEFCREYGVQEGGMQQLRSEVAENMRR
                     ELATNVRARLRQQLLDKLLEANPVEVPQGLVDAQVREMQLDTARRIGAKDASQVPPAD
                     PFVAPARRRVALGILVGELIKTRGIALDRARVEDRLADIAGGYPDAESVLKAYRQNAE
                     AMRQVENLVLEDQVVDYLLERAKVTDQAATFKDVMNFGA"
BASE COUNT          411 a          810 c          868 g          390 t
ORIGIN      
        1 tgccggcgac acacgcgccg ccgccgagtg cgccgcgcta gtggatgtac ccgtgtcgat
       61 gcaggtcctt tgcgatcgat tcggcgactt tgtccatgcc gacgtcggtc tcctcgatgt
      121 gcttcaccgt cgtgtcgacg gtgtcgagga gcgtcgcact gctggcgtcg aacacgcgcg
      181 tcgcgacgtg gacctcgcct tcgagcaggt tcaccggcgg ccactgttcg aagtccgtgt
      241 agacgaccgg cacgccccac acgccccagt agtccggcgc aaagccgagt ccggcctcct
      301 tgtactgcgc gccgccacgc gtgtcgcggt cgtcaccctg ctcgacctgc tgttgcttcg
      361 aaacgagcac cgtcgccagc acgccgtcgg cctgaaccgc tgcgaccgcc ttgtcgatgc
      421 tctcacgggt gagggggttg atctggtcga ggacgtcgca gctcgcgtag gcctgggttt
      481 ccttgccggt gatctgtgag gcgaggaact gctcgaactg gcagcgtcgg tcctcgttcg
      541 ggctgacgcc aacgaccagt acgcgcgcaa aggggaggct ggcgcctgcg gctgccgctt
      601 catgggtctg catcgcggaa ccggccagca cgaccagtcc gagcgcgaaa gcccaggtgt
      661 ggacaaccga ccgcctgcgg gtcaccgagc gctgggcagt gcgggtcagg cgagcgtgtc
      721 ggggctgcag catgttgggc tttcctgggc cgttggcgag cgtgagtcga tggggacggg
      781 atcttatcaa tcggatgggc gcttccaacg actgtttcag gccggccgtg gtgcgcggca
      841 cgcagtcgta ccccggccgc ggtgcccgac acgcagtcgt gggccgtctc gggcgcggca
      901 gggtgccccg ggggatgtcg ggcgatcgcc aggtccggct tacggcttga tttacctata
      961 gaatccgccc cgttcgcccg cccgtgcggg cggtcggcga aagtggcgga actggtagac
     1021 gcgctggatt taggttccag tgggtaaccc ccgtgagggt tcgagtccct cctttcgcac
     1081 cagccctccc gacgcgcact ggagggcgtg attccgtggc aaaatacccg gctttcccgg
     1141 atggccgcag gccgtcctga ccatcttccg aggaccgacg agcatgcagg tttccgtgga
     1201 gcagacgggc gcgctggagc gccgcatgga agtgagcgtt ccgaaggagc gcgtcgagca
     1261 ggcggtgcac aagcgcctgc agcaggtggg ccgcaccgcg aaactcaagg gctttcgccc
     1321 cggcaaggcc ccgctcaagg tcatccagca gcagttcggt gcgcaggtgc gccaggaggt
     1381 cgtcggcgac ctgatgcagt cgagcttcgc cgaggccatc acgcagcaca acctgtcccc
     1441 ggccgcgggt ccgcgcatcg agccgatcac catgacgccc ggcgaggacc tcaagtaccg
     1501 cgcgatcttc gaggtgtacc ccgaggttgc gctggcaagc gtggagggcc tcgacgtcat
     1561 ccgcccggtg gccgaggtga cggaaggcga catcgacgcc atggtcgaga acctgcgctc
     1621 gcagcgcccg aagtttgaga ccgtcgagcg cgagagccgc gagggcgatc gcgtcacgat
     1681 ggatttcgtg ggcctgctcg acggcgccga gttcgagggc agcaagggca ccgacgtggc
     1741 cgtgctgctc ggcagcgggc gcatgatcaa ggagttcgag gcgggtatca ccggaatgac
     1801 ggccggcgag cagcgcaaga tcgacgtccg ctaccccgac gactaccaca acaagtcgct
     1861 ggcgggacga accgcgaact tcgacgtgca catgaagaag gtcgaggaga agcgcctgcc
     1921 ggacctcgac gacgagttct gccgcgagta cggcgtgcag gaaggcggga tgcagcagct
     1981 gcgcagcgaa gtcgccgaga acatgcggcg cgaactcgcg accaacgtgc gcgcgcggct
     2041 gcgccagcaa ctgctcgaca agctgctcga ggccaatccg gtcgaggttc cgcagggcct
     2101 cgtcgacgcg caggtgcgtg agatgcagct cgacacggcg cgtcgcatcg gcgccaagga
     2161 cgcctcgcag gttccgccgg cggatccgtt cgtcgcgccg gcacgccgcc gcgtcgcact
     2221 cggcatcctg gtcggcgagc tgatcaagac gcgcggcatc gccctggacc gcgcacgggt
     2281 cgaggaccgc ctcgccgaca tcgcgggtgg ctacccggat gccgagtccg tgctcaaggc
     2341 ataccggcag aacgccgagg ccatgcgcca ggtcgagaac ctcgtgctcg aggaccaggt
     2401 cgtcgattac ctcctcgagc gcgccaaggt caccgaccag gcggcgacgt tcaaggacgt
     2461 gatgaatttc ggcgcctga
//
LOCUS       DAOMFD010000270         2439 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_24286_length_2439_cov_34.723154, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000270 DAOMFD010000000
VERSION     DAOMFD010000270.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24286_length_2439_cov_34.723154"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1053
                     /locus_tag="PL152_10810"
     CDS             <1..1053
                     /locus_tag="PL152_10810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HQR49817.1"
                     /translation="VPEPAADEVVVRIEATPINPSDLGLLLGAADMTTAQQSGTAERP
                     VVTARIPEGLMKAMASRVDQSMPVGNEGAGTVVTAGSSPAAQALLGKVVGTFGGAMYA
                     QYRCIKAAQCMALPAGTSAAEGASCFVNPMTALGMVETMRREGHKALVHTAAASNLGQ
                     MLNRLCIEDGIGLVNVVRSEAQEKILRDLGAKHVCNSGAPTFLRDLTDAIAATGATLA
                     FDAVGGGPLAGQILGCMEAAITRSAGEYLRYGSTTHKQVYIYGALDLGPTTLKRNFGA
                     AWGVGGWLLMPFLQKVGPEVTRKLQARVASGLKTTFASHYTQEVSLAGALSLDAIAAY
                     ARRSTGEKFLINPAAG"
     gene            1082..1918
                     /locus_tag="PL152_10815"
     CDS             1082..1918
                     /locus_tag="PL152_10815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR49818.1"
                     /translation="MKLKNKVAVVTGAAGGLGKAIAVAMAKEGASVVACDVNEQGLAA
                     AAKEAGAECAAHGATCMAVRCDVSSSADVARMFRDAAERFGTVHVLVNNAALVPSRPE
                     DEVRRRRHYDYLTTPVPRRSLELTSSLTDEDWHRYWGVNVHGVFYCTREALRLMEPRR
                     EGKIVNIASVAGISAYSAHSPHYTATKGAVVAFTRAVALDVAGANIFVNAIAPGGVLT
                     PDFAAYMERSSEAERNRFNQLVPLGRLGTVEEYASLAVYLASDGHYLVGQIISPNGGL
                     VI"
     gene            2081..>2439
                     /locus_tag="PL152_10820"
     CDS             2081..>2439
                     /locus_tag="PL152_10820"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HQR49819.1"
                     /translation="MYFDDDALLPENQDELIICAAPYGPEWMPSDFPEDIAVTLDEQV
                     QKAVDCYNAGATVLHVHVREADGKGSKRLSMFNELLARLRDAVPKMVLQVGGSISFSP
                     EGEGDQAKWLGYDTRHLL"
BASE COUNT          430 a          795 c          841 g          373 t
ORIGIN      
        1 gtgccggagc cggccgccga cgaggtcgtg gtgcgcatcg aggcgacgcc gatcaacccc
       61 tcggacctcg ggctgctgct cggtgcggcc gacatgacca ccgcgcagca gtcgggcact
      121 gcagagcgtc cggtcgtcac tgcgcgcatc ccggaaggac tcatgaaggc catggcgtcg
      181 cgcgtcgacc agtcgatgcc cgtcggcaac gagggtgcgg gcaccgtcgt gacggccggc
      241 agttcaccgg ccgcacaggc gctgctcggc aaggtcgtcg ggacgttcgg cggtgcgatg
      301 tatgcacagt atcgctgcat caaggctgcc caatgcatgg cgctgccggc gggcaccagc
      361 gccgccgaag gcgcatcgtg cttcgtcaat ccgatgaccg cactcggcat ggtggagacc
      421 atgcggcgcg aggggcacaa ggcgctggtc cacacggccg cggcatcgaa tctcggacag
      481 atgctgaatc gcctgtgcat cgaggacggc atcgggctcg tgaacgtcgt gcgcagcgag
      541 gcgcaggaga agatcctgcg tgacctgggc gcgaagcacg tgtgcaactc cggcgccccg
      601 accttcctgc gcgacctgac cgacgccatc gccgcgaccg gtgcgacgct ggccttcgac
      661 gccgtcggcg gcggtccgct ggcggggcag atcctcggct gcatggaggc ggccatcacg
      721 cgctcggccg gcgaatacct gcgctacggg tccacgaccc acaagcaggt ctatatctac
      781 ggcgcgctcg acctcggccc gacgacactc aagcgtaact tcggtgccgc ctggggcgtc
      841 ggcggctggc tgctgatgcc gttcctgcag aaagtcgggc cggaagtcac ccgcaagctg
      901 caggcccgcg tcgcgagcgg actgaagacg accttcgcga gccactacac gcaggaagtc
      961 tcgctggccg gcgcactctc gctcgacgcg attgcggcgt atgcgcggcg ctcgaccggc
     1021 gagaaattcc tgatcaatcc agcggcggga tgaatgccgc gactgtgaca ggggtccggc
     1081 gatgaaactg aagaacaagg tggccgtcgt gacgggcgcg gccggtggcc tcggcaaggc
     1141 catcgccgtt gccatggcga aagagggggc gagcgtcgtc gcctgcgacg tcaacgagca
     1201 ggggctggcc gcggcggcga aagaggccgg tgccgagtgc gctgctcacg gcgcgacatg
     1261 catggccgtg cgctgcgacg tgtcctcgag tgccgacgtc gcgcgcatgt tccgcgatgc
     1321 cgccgagcgc ttcggcacgg tccacgtcct ggtcaacaac gcggcgctcg tgccctcgcg
     1381 tcccgaggac gaagtgcgcc gccgccgtca ctacgattac ctgacgacgc cggtgccgcg
     1441 tcggtcactc gagctcacga gcagcctcac cgacgaggac tggcatcgtt actggggcgt
     1501 caacgtgcac ggtgtcttct actgcacgcg agaggccttg cggctcatgg agccgaggcg
     1561 cgagggcaag atcgtcaata tcgcgtcggt ggccggtatc tcggcgtaca gcgcgcacag
     1621 cccgcattac accgcgacca agggcgcggt cgtggcattc acgcgggccg ttgccctcga
     1681 cgtcgccggc gccaacatct tcgtcaatgc gatcgcgccc ggtggcgtgc tgacgccgga
     1741 tttcgccgcg tacatggaac gttcgagcga ggcggagcgc aatcgcttca accagctggt
     1801 gccgctcggc cggctcggca cggtcgaaga gtacgcgtcg ctggccgtct atctggcgag
     1861 tgacggtcac tacctggtcg ggcagatcat cagcccgaac ggcgggctcg tcatctgagc
     1921 ccggtcggca gtccatgagt cggttcgggg cgctcgccac cggttgcgca gcgcaagaaa
     1981 aagatacgaa atgataatat aaagcaacgg taaggtgatt tataacatgg tcctgaggta
     2041 atactgagaa aacctcgcaa caaccacagg atccacgcca atgtacttcg acgacgacgc
     2101 gctgctcccg gagaaccagg acgaactgat catctgcgcc gcgccgtacg ggccggagtg
     2161 gatgccgagc gactttcctg aagacatcgc cgtgaccctg gacgagcagg tccagaaggc
     2221 ggtcgactgc tacaacgccg gcgcgacggt gctgcacgtt cacgtgcgcg aggcggacgg
     2281 caagggcagc aagcgcctgt ccatgttcaa cgagctgctc gctcgcctgc gcgacgccgt
     2341 gccgaagatg gtgctgcagg tcggcgggtc gatctccttc tcgccggagg gcgagggcga
     2401 ccaggccaag tggctcggct acgacacgcg gcacctgct
//
LOCUS       DAOMFD010000271         2423 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_24589_length_2423_cov_28.903716, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000271 DAOMFD010000000
VERSION     DAOMFD010000271.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24589_length_2423_cov_28.903716"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="PL152_10825"
     CDS             complement(<1..362)
                     /locus_tag="PL152_10825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HQR49820.1"
                     /translation="MDQFAKETLPVSLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLFAMHEANNHWNRSYVKCARVVGEVMGKYHPHGDTAIYDTLVRMAQDFSLRYML
                     IDGQGNFGSIDGDNAAAMR"
     gene            597..1946
                     /locus_tag="PL152_10830"
     CDS             597..1946
                     /locus_tag="PL152_10830"
                     /inference="COORDINATES: protein motif:HMM:NF006549.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family hydrolase"
                     /protein_id="HQR49821.1"
                     /translation="MKPVDLVIEARWVVPIVPRAAVLEDHAVVVAGGRIVDLLPTTEA
                     RVRFAPAERVALPTHALIPGLVNLHTHAAMALFRGIGDDLPLMQWLQKRIWPLEKALA
                     SPEFVHDGSRLAALEMVRAGTTCCSDMYFYPESAVRGLREVGMRAVVGIIAIEFPTAY
                     AADAEDYLRKGLATRDLFREDPLVAFTLAPHAPYTVSDDTLRRIAVLAEELDLPVHMH
                     LHETAQEIEESLRLHGERPLARLERLRLVTERLIAVHAVHLESDEIALLAQRGASVAH
                     CPASNLKLGSGIAPVAELLAAGVNVGIGTDGAASNDRIDMVSELRLAALLAKGVRRDP
                     SALPAPAALEAATLGGARALGLERRIGSIEPGKEADLAAFDLSSLETQPVFDAMSQLV
                     YAAGREHATDVWVAGRPVVRMRQVLAEAQVREPGQTAALVHAWQNRCRQALQTAGLD"
     gene            2041..>2423
                     /locus_tag="PL152_10835"
     CDS             2041..>2423
                     /locus_tag="PL152_10835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49822.1"
                     /translation="MKQSIKLGLVASALMAITGTVSAQMKDITVVDTKESPYVIDVRG
                     VIARDPFGLCWRTGSWSAERAAKTVIKDAKYPNWTAICECEDEALWKTLPPACLPPPP
                     PPPPPPPPPPPPPPAPAPTSEKVTFA"
BASE COUNT          391 a          825 c          800 g          407 t
ORIGIN      
        1 cgcatcgccg cggcgttgtc gccgtcgatc gagccgaagt tgccctggcc gtcgatcagc
       61 atgtagcgca gcgagaagtc ctgcgccatc cgcaccagcg tgtcataaat ggcagtgtcg
      121 ccgtgcgggt ggtacttgcc catcacctcg ccgacgacgc gggcgcactt cacataggag
      181 cggttccagt gattgttcgc ctcgtgcatc gcgaagagca cccggcgatg caccggcttc
      241 aggccgtcgc gcacgtcggg cagcgcgcgc ccgacgatga cgctcatcgc gtagtccaga
      301 tacgagcgcc gcatctcctc ttcgagcgag actggcaggg tttccttcgc gaattgatcc
      361 atcgaggcgg gcggcttgag caggccaggg acattccttc cctggctgct ctctactctg
      421 gaaagcaatc gaaaattcta ccagcccgag cgcgctcgcc cggacgggga agcacttgcc
      481 gtggtgtccg cgcgagcggc caaccacgcg gtcggacgga gcactcgcca accctggcgc
      541 gttgtgctct catgcgccgc ccgcgttcca acccatcacg tgacccattc aacgacatga
      601 aacccgtcga tctggtgatc gaggcccgct gggtcgttcc gatcgtgccg cgcgccgccg
      661 tgctggaaga ccacgcggtg gttgtcgccg gcggccgtat cgtcgatctg ctgcccacga
      721 ccgaggcgcg cgtacgcttc gcccctgccg aacgggttgc gttgccgacc catgctttga
      781 tccccggtct ggtgaacctg catacccatg cagcgatggc gcttttccgg ggcatcggtg
      841 acgacctgcc cctgatgcag tggctgcaga agcgcatctg gccgctggaa aaggcactgg
      901 cgtcgccgga gttcgtgcac gacggctccc ggcttgccgc cctggaaatg gtgcgggctg
      961 gcacgacctg ctgctcggac atgtacttct atcccgagtc cgccgtgcgt gggctgcgcg
     1021 aagtcggcat gcgggctgtc gtcggcatca tcgcgatcga gtttccgacg gcgtatgcgg
     1081 cggacgccga ggactatctg cgcaagggcc tcgcgacgcg cgacctgttc cgcgaagacc
     1141 ccctggtggc cttcaccctg gcgccgcacg cgccctacac ggtgagcgac gacaccctga
     1201 ggcgcattgc agtgctcgcc gaagaactcg acctgccggt gcacatgcac ctgcacgaaa
     1261 ccgcgcagga gatcgaggag tcgctgcggc tgcatggcga gcgtcccctg gcgcgcctgg
     1321 agcggctgcg cctggtcacg gagcgcctga tcgccgtgca cgcagttcac ctcgagtcgg
     1381 acgagattgc cctgcttgcc cagcgcggcg cctcggtggc gcactgcccg gcgtccaacc
     1441 tgaagctcgg cagcggcatc gcgccggtcg cagagctgct cgcggccggc gtaaacgtgg
     1501 gcatcggcac cgacggcgcc gccagcaacg accgcatcga catggtgtcg gaactcaggc
     1561 tcgccgccct actcgcgaaa ggcgtccgcc gcgacccgag tgccttgccg gcgcccgcgg
     1621 cactcgaggc cgccacgctc gggggagccc gggcgctggg gctggagcgg cgcatcggct
     1681 cgatcgagcc gggcaaggag gccgatctgg ccgccttcga tctctcaagt ctcgaaaccc
     1741 agcccgtctt cgatgccatg tcgcaactcg tgtacgccgc gggtcgcgag cacgcgaccg
     1801 atgtgtgggt ggccggtcga cctgtggtca gaatgagaca ggtgcttgcg gaagctcaag
     1861 tcagggagcc cggccagacg gcagcccttg tgcatgcgtg gcaaaatcgc tgtcggcaag
     1921 cgcttcagac ggcggggttg gattgacttt cgggtcgcca tcatcggccg atgcggtgcg
     1981 agtcataccc ggtcgagttg gtaccccggc cggtcttcct tatctcgagt gaggagcaat
     2041 atgaagcaat ccatcaagct gggcctcgtg gcatcggcac tgatggccat cactgggacg
     2101 gtttctgccc agatgaagga catcaccgtc gtcgatacca aggagagccc gtatgtaatc
     2161 gatgtgcggg gcgtcatcgc ccgcgatccg ttcggcctgt gctggcgtac gggatcgtgg
     2221 agcgcggaac gcgctgcgaa gacggtcatc aaggacgcca agtatcccaa ctggaccgcg
     2281 atctgcgagt gcgaggacga agcgctgtgg aagacgttgc cgccggcctg cttgccgccg
     2341 ccgcccccac cgccgccccc gccgccgccc ccgccgcccc cgccgccagc accggcgccg
     2401 acgagcgaga aggtgacgtt cgc
//
LOCUS       DAOMFD010000272         2415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_24718_length_2415_cov_34.140254, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000272 DAOMFD010000000
VERSION     DAOMFD010000272.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2415
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24718_length_2415_cov_34.140254"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="PL152_10840"
     CDS             <1..338
                     /locus_tag="PL152_10840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49823.1"
                     /translation="LDELEWVQENERLTLRQKERAVDDLIELAGAVDGILQAQADADA
                     AYFLAIAGRRFNGEEEAGIRAMVLRAYRWQYILSGVQHPHFGRLLTGMTTPQQLARIQ
                     SALAPIAAA"
     gene            370..495
                     /locus_tag="PL152_10845"
     CDS             370..495
                     /locus_tag="PL152_10845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49824.1"
                     /translation="MTTGTTLERYEVIVCAALAVAVGLFTTLLFAHVLFSAPMVA"
     gene            689..1552
                     /locus_tag="PL152_10850"
     CDS             689..1552
                     /locus_tag="PL152_10850"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HQR49825.1"
                     /translation="MNRSKVIQSVGFAAGVLAAFMCVSAAAATYGGPQSGANPNLRSS
                     AFYIVDTADSKVLAARNEQVAAPIASITKLMTALVVLDAGQPMDEMISITREDVRETA
                     KSGSRLSQGVRLSRADLLHLALMSSENRAAHALCRSYSGGVPACVAAMNAKARSLGMS
                     SAHFVEPTGLSSNNVSSAVDLAKLVQAAAANATIREYSTDPEHTFYVRGHAMAFRSTN
                     ALVKKSDWRVTVQKTGYIAEAGRCLVMQTIIDGRDVVIVLLNSWGRLTRIGDANRVRD
                     WMEAYHPSTSG"
     gene            1632..>2415
                     /locus_tag="PL152_10855"
     CDS             1632..>2415
                     /locus_tag="PL152_10855"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane dipeptidase"
                     /protein_id="HQR49826.1"
                     /translation="MPINRREFVSLTVATASWLLTRSSNAGIPPVTAMYTRSIVIDGL
                     GSPGGLGTDKGAALTAAQVEDVRASGMTGFNVTVGAVGTMPSLEAFEKIVRDVARWEG
                     EIDRSPTVLARVRTAGDIQKAKMGGKSGLIYGLQDGVSFEDDLDRLTALHQLGLRVIQ
                     PTYNRRNLLGDGCMEPADAGLSTAGRAAIERMNSLGILVDLSHCGRRTASDAIALSRK
                     PVAFTHTGCFAVAEHPRNRTDAELKAVAEKGGVCGIYVMPYLS"
BASE COUNT          387 a          820 c          828 g          380 t
ORIGIN      
        1 tgctcgacga gctcgagtgg gtgcaggaga acgagcgcct gacgctgcgc cagaaggagc
       61 gggcggtcga cgacctgatc gagctggccg gtgcggtcga cgggatcctg caggcccagg
      121 cggacgccga cgccgcctat ttcctcgcga tcgcgggtcg ccgcttcaac ggcgaggaag
      181 aggcgggcat ccgcgcaatg gtccttcggg cttaccgctg gcagtacatc ctgtcgggcg
      241 tgcagcaccc gcacttcggc cgcctgctga cgggcatgac cacgccgcag caactggccc
      301 gcatccagtc ggcgctggca cccatcgccg ccgcgtgatt cacgtcgatt caaccgggag
      361 gaaccgatca tgaccactgg aaccactctc gaacgctatg aagtcatcgt ctgtgccgcg
      421 cttgcggtgg cggtcgggtt gttcacgacc ctgctgtttg cccacgtgct gttcagcgca
      481 ccgatggtgg cgtgacgcga ccccgggccg gccttccgtg gcggcgactc cgcggcgggc
      541 cggcccgatt ttcgaggatt tgcgcacact tgcgacaaat tcctacgtga cgacggacct
      601 gaaactgggt acccttcgcg ccggtcgacg tccggcgcgg tggtcgcgag cgggtcgggg
      661 acccgtggcg gacgaggggg tacctctctt gaatcggtcg aaagtgatcc agtcggtggg
      721 gttcgcggca ggcgtcctgg ccgcgttcat gtgcgtctct gcggcggcgg ccacctacgg
      781 cgggccgcag tcgggcgcga acccgaacct ccggtccagc gcgttctaca tcgtagacac
      841 cgccgattcg aaggtgctcg ccgcgcgcaa tgagcaggtc gcggcaccca ttgcctccat
      901 caccaagctc atgaccgcgc tcgtggtgct cgacgccggg cagccgatgg acgagatgat
      961 ctccatcacg cgcgaggacg tgcgcgagac cgccaagagc ggatcgcggc tgtcgcaggg
     1021 cgtgcgcctg agccgcgccg atctcctcca cctggcattg atgtcgtccg agaaccgggc
     1081 cgcgcatgcg ctgtgccgca gttactcggg cggcgtgccg gcgtgcgtcg ccgcaatgaa
     1141 tgccaaggcg cggtcgctcg gcatgagctc tgcgcacttc gtcgagccga cgggactgtc
     1201 gagcaacaac gtgtcgagcg ctgtcgacct cgccaagctg gtgcaggccg ccgccgcgaa
     1261 cgcgaccatc cgggaatact cgaccgatcc ggagcacacc ttctacgtgc ggggacacgc
     1321 catggcgttc cgcagcacga atgcgctggt caagaaatcc gactggcggg tgacggtgca
     1381 gaagaccggc tacatcgccg aggccggtcg ctgcctggtt atgcagacca tcatcgacgg
     1441 gcgcgacgtg gtcatcgtgc tgctcaactc gtggggccgg ctcacgcgca tcggcgacgc
     1501 caatcgtgtc cgcgactgga tggaagcgta ccacccgtcg acgtcgggct aggcgcatcc
     1561 cggcggcgga ttcccgccgc ggccagtcct tggggtaagc tcgggcgccg agacgcccag
     1621 ctgctccgca catgcccatc aaccgccgcg agttcgtatc cttgaccgtc gcgacggcct
     1681 cgtggctcct gacgcgctcc agcaacgcgg ggatcccgcc ggtcaccgcc atgtacacac
     1741 gcagcatcgt catcgacggg ctcggctccc cgggcggtct cggcaccgac aagggcgcag
     1801 ccctgactgc ggcccaggtc gaggacgtgc gcgcttcggg catgaccgga ttcaacgtca
     1861 cggtcggcgc ggtcggcacg atgccgtcgc tcgaggcgtt cgagaagatc gtgcgcgacg
     1921 tcgcgcgctg ggaaggcgag atcgaccgca gcccgacggt gctcgcgcgc gtccgcacgg
     1981 ccggggacat ccagaaggcg aagatgggcg ggaagtccgg attgatctac ggactgcagg
     2041 acggcgtgtc gttcgaggac gacctcgatc ggctgacggc gctgcaccag ctcggtctca
     2101 gggtcatcca gccgacgtac aaccgccgca acctcctcgg cgacgggtgc atggagccgg
     2161 ccgacgcggg cctgagcacc gcgggacggg ccgcgatcga gcgcatgaat tcgctcggca
     2221 tcctcgtgga cctgagccac tgcggccgcc gcacggcctc cgatgcgatc gcactctcgc
     2281 gcaagccggt tgcgttcacc cacacgggct gcttcgcagt cgccgagcat ccgcgtaatc
     2341 gcaccgatgc cgagctgaag gccgtggcgg agaagggcgg cgtctgcggc atctacgtca
     2401 tgccgtacct gtcga
//
LOCUS       DAOMFD010000273         2399 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_25017_length_2399_cov_36.439420, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000273 DAOMFD010000000
VERSION     DAOMFD010000273.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2399)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2399
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25017_length_2399_cov_36.439420"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="PL152_10860"
     CDS             complement(<1..368)
                     /locus_tag="PL152_10860"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HQR49827.1"
                     /translation="MDASTTAGGWSPTSWTTKPASQQAPYRDPAALARAVEALGRLPP
                     IVVSWEVEALKKEIAEAQAGRRFLLQGGDCAESFDECESSHIAAKLKILLQMSLVLLH
                     GLKKPVIRVGRFAGQYAKPRS"
     gene            521..1690
                     /locus_tag="PL152_10865"
     CDS             521..1690
                     /locus_tag="PL152_10865"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HQR49828.1"
                     /translation="MHKVLVLGAGKIGSLISGLLAASGDYDVHVADVNETVARAVVDA
                     HGLDNLEAHGFDAGDAKALSAHLRAHPVEAIISSLPYYCNVAVAEAARAAGAHYFDLT
                     EDVAVTRGVRRVADGADTAFVPQCGLAPGFISIAANALIGHFDELRSVKLRVGALPQH
                     PNNVLKYSLTWSTEGLINEYGNPCECIANGRSVESAPLEGLEEIEIDGTLYEAFNTSG
                     GLGSLGDTYGQRAETMDYKTIRYPGHCAQMRLLMNDLKLNQDRATLKRILENAVPQTL
                     QDVVIVYVAVTGRQAGELREENYVSKIYPQLIAGRLWSAIQVTTAAGVTAVVDLVLRT
                     PGRYRGFVRQEDFRLVDVLDNRFGRHYADVGSKEVSGRMVVAGHTGQQRAANGGK"
     gene            1690..>2399
                     /locus_tag="PL152_10870"
     CDS             1690..>2399
                     /locus_tag="PL152_10870"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR49829.1"
                     /translation="MDKVLANLGLESLNPGTWCANGGWLADPAAPRVESVNPANGKVI
                     ATVLATTAAQYEQVMASAREVAAAWRTVPAPKRGEAVRLVGDELRRHKSDLGSLVSLE
                     MGKIKAEGDGEVQEMIDIADFAVGQSRMLYGLSMHSERPSHRMFEQWHPLGVVGVISA
                     FNFPVAVWSWNAFLAAICGNVTVWKPSLKTALCAVAVQHLANRVLERHGYPGIFQMFV
                     DATHELATRFVDDRRVDLV"
BASE COUNT          396 a          853 c          797 g          353 t
ORIGIN      
        1 gagcgcggct tggcgtactg gccggcgaag cgcccgaccc ggatgaccgg cttcttcagc
       61 ccgtgcagca gcaccaggct catctggagc aggatcttga gcttggcggc gatgtgcgag
      121 gactcgcact cgtcgaacga ctccgcgcag tccccgccct gcaggaggaa ccgccggcct
      181 gcctgggcct cggcgatctc cttcttgagg gcctcgacct cccacgacac cacgatcggc
      241 ggcaaccggc cgagggcctc gaccgcgcgc gccagcgccg ccggatcccg gtacggcgcc
      301 tgctgggatg cgggcttggt ggtccagctg gtgggcgacc agccgccggc cgtcgtcgaa
      361 gcgtccatcg tgcaatccta accgttaacg ctgcagtgca gtaggacccc cgggggtgac
      421 gatgcgtcgc gatttcgtca gaatcgcgcc cccgaaacgt aatttccgct cgccaggccg
      481 tcccggcgcg accggaccaa gaaaaccctg gagtcctcgc atgcacaagg tcctcgtcct
      541 cggggccggc aagatcggtt cgctgatctc cggcctgctc gccgccagcg gcgactacga
      601 cgtgcacgtc gccgacgtga acgagaccgt cgcgcgcgcc gtcgtggacg cgcacggcct
      661 ggacaacctc gaggcacatg gcttcgacgc cggagacgcc aaggcgttgt ccgcgcacct
      721 gcgcgcgcac ccggtcgagg cgatcatctc gagcctcccc tattactgca acgtggcggt
      781 cgccgaagcg gcgcgcgccg ccggcgccca ctacttcgac ctgaccgagg acgtcgcggt
      841 tacgcgcggc gttcgccgcg tcgccgacgg cgcggacacg gccttcgtgc cgcagtgcgg
      901 cctggccccc ggcttcatca gcattgcggc caatgcgctc atcggccact tcgacgagct
      961 gcgcagcgtg aagctgcgcg tgggtgcgct gccgcagcac ccgaacaacg tcctcaagta
     1021 ttcgctcacc tggtccaccg agggactcat caacgaatac ggcaacccct gcgagtgcat
     1081 cgcgaacggc cgctcggtcg agtccgcgcc cctcgagggg ctcgaggaga tcgagatcga
     1141 cggcacgctg tacgaggcgt tcaacacctc gggcggactc ggctcgctgg gcgacaccta
     1201 cgggcagcgc gccgagacga tggactacaa gaccatccgc taccccgggc actgcgcgca
     1261 gatgcggctg ctcatgaacg acctcaagct caaccaggac cgcgccacgc tcaagcgcat
     1321 cctcgagaac gccgtgccac agacgctgca ggacgtcgtg atcgtctacg tcgcggtcac
     1381 cgggcggcag gcgggcgaac tgcgcgagga gaactacgtg agcaagatct acccgcagct
     1441 catcgccggg cgcctgtggt ccgcgatcca ggtgaccacc gccgccgggg tcacggccgt
     1501 cgtggacctc gtgctgcgca cgccgggacg ctatcgggga ttcgttcgcc aggaggactt
     1561 ccgcctcgtg gacgtgctcg acaaccgctt cgggcggcat tacgcggacg tcggcagcaa
     1621 ggaagtctcg ggacgcatgg tggtcgccgg ccatacgggc cagcagcgcg ccgcaaacgg
     1681 aggcaagtga tggacaaggt actcgccaac ctcgggctcg agtcgctcaa tcccggaacg
     1741 tggtgcgcga acggcgggtg gctcgccgat cccgccgccc cgcgcgtcga atccgtgaat
     1801 cccgcgaacg gcaaggtgat cgcgacggtg ctcgccacga ccgccgcgca gtacgagcag
     1861 gtcatggcgt ccgcacgcga ggtcgcagcg gcctggcgaa ccgtgccggc gcccaagcgc
     1921 ggcgaggcgg tgcgcctcgt cggtgacgaa ctgcgccgcc acaagtccga cctcggcagc
     1981 ctggtgtccc tcgagatggg caagatcaag gccgagggcg acggcgaggt gcaggagatg
     2041 atcgacatcg ccgacttcgc cgtcggccag tcgcgcatgc tgtacggcct gtccatgcac
     2101 tcggagcgcc cgagccaccg catgttcgag cagtggcacc cgctcggcgt ggtcggcgtc
     2161 atcagcgcgt tcaacttccc cgtggcggtg tggtcgtgga acgcattcct cgcagccatc
     2221 tgcggcaatg tcaccgtctg gaagccgtcg ctcaagaccg cgctctgcgc ggtcgccgtg
     2281 cagcacctcg ccaaccgggt gctcgagcgc cacggttacc cgggcatctt ccagatgttc
     2341 gtggacgcga cccacgaact ggcaacgcgc ttcgtcgacg accggcgcgt cgacctcgt
//
LOCUS       DAOMFD010000274         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_25191_length_2390_cov_33.126767, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000274 DAOMFD010000000
VERSION     DAOMFD010000274.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25191_length_2390_cov_33.126767"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..701)
                     /locus_tag="PL152_10875"
     CDS             complement(<1..701)
                     /locus_tag="PL152_10875"
                     /inference="COORDINATES: protein motif:HMM:NF038402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HQR49830.1"
                     /translation="MRRLAAALALGALALAADAAQPLRIVSLAPNLTELAYAAGAGDR
                     LVGTVDYSDYPDAARALPRVGDAWRVDPERVLELRPDLVLAWSSGTPGDTVERLRSLG
                     LRVVSVPTFRIADVAAALRTIGRIAGTEPVANGAAEAFEGEMRRLRETHVGARPLGVF
                     IEIDDEPLYTVNGRHIISEIVELCGGRNVFADLPQLAPPVALEAVLAKDPQVILTTDD
                     TIADPRSPWRQWPRMA"
     gene            complement(698..1603)
                     /gene="ampE"
                     /locus_tag="PL152_10880"
     CDS             complement(698..1603)
                     /gene="ampE"
                     /locus_tag="PL152_10880"
                     /inference="COORDINATES: protein motif:HMM:NF028423.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory signaling modulator protein AmpE"
                     /protein_id="HQR49831.1"
                     /translation="MSLIALLLAFLIEHGLTDALRLREPRWLDHYCDWAGRRLAGLGG
                     PTLIILVTLFVLLPVVPVAVAYDLIGSHLLGLLQLAFAVLVLLVSYGPRDLQDEVDDY
                     LDALSRGDRAEAERLAKELLESDAAGRGAGLREAVEQAIFVQANNRIFGVVFWFMVAG
                     PAGAWLFRVSDLMRRRAVFEAVRQGTPAAERRFGAALDFVHGLLAWIPARLAAVGYAL
                     AGSFEDAVGNWRAAVGEVEHGLLGRSEELLARVGKGSLQPSLADAAPDALESAAAGGA
                     WRIVSRTIWIWLAVIALLVLAGSVA"
     gene            complement(1600..2211)
                     /gene="ampD"
                     /locus_tag="PL152_10885"
     CDS             complement(1600..2211)
                     /gene="ampD"
                     /locus_tag="PL152_10885"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HQR49832.1"
                     /translation="MRTSLARAAGRSELDVDAGTGWLSGARRVPSPNCDDRPEGVAVD
                     LIVVHGISLPPGEFGGPWIDALFTNSLPPDAHPYFEQVAGLRVSAHALVRRDGEVVQY
                     VPFQRRAWHAGASSWQGRERCNDYSVGIELEGTDASAYEPAQYEVLARLVALLCRAYK
                     GLSTERVVGHSDVAPGRKSDPGIAFDWPLLRARARYELEVAPA"
BASE COUNT          375 a          918 c          811 g          286 t
ORIGIN      
        1 gccatccgcg gccactgccg ccacggcgac cgcgggtccg cgatcgtgtc gtcggtcgtc
       61 aggatcacct gcgggtcctt cgcgagcacg gcttcgagcg ccaccggcgg ggccagctgc
      121 ggcaggtccg cgaacacgtt gcgcccgccg cacaattcca cgatctcgct gatgatgtgc
      181 cggccgttga ccgtgtacag cggctcgtcg tcgatctcga tgaacacgcc gagcggccgc
      241 gcgccgacgt gcgtctcgcg caggcggcgc atctccccct cgaacgcctc ggcggcgccg
      301 ttcgcgacag gctcggtgcc cgcgatgcgc ccgatcgtgc gcagcgcggc cgccacgtcg
      361 gcgatgcgga acgtcgggac ggacacgacc cgcagcccca gcgaccggag gcgctcgacc
      421 gtgtcgccgg gcgttccgga cgaccaggcc agcaccaggt ccggcctcag ctccaggacc
      481 cgctcgggat ccacgcgcca tgcatccccg acccgcggca gggcgcgcgc cgcatccggg
      541 tagtcgctgt aatccacggt gccgacgagc cggtcgcccg caccagcggc gtatgcaagc
      601 tcggtgagat tgggtgcgag gctgacgatc ctgagcggct gggctgcgtc cgcagcaagc
      661 gcgagcgcgc ccagcgcgag cgcggccgcg agccgcctca cgccacgctg cccgcgagca
      721 ccagcagcgc gatcaccgcc agccagatcc agatggtgcg cgacacgatg cgccacgcgc
      781 cgccggcggc agccgattcg agcgcgtccg gcgccgcgtc ggcaagactc ggctgcagcg
      841 atcccttgcc gacgcgcgcc agcagctcct ctgagcggcc gagcaggccg tgctcgacct
      901 ccccgaccgc ggcgcgccag ttgccgacgg cgtcctcgaa gctgcccgcg agcgcgtagc
      961 ccaccgctgc aagtcgcgcc gggatccacg ccagcagtcc gtggacgaaa tcgagcgcgg
     1021 cgccgaatcg gcgctcggcc gccggcgtcc cctggcgcac cgcctcgaac acggcacgac
     1081 gccgcatcag gtcgctcacc cggaacagcc acgcgcccgc gggcccggcg accatgaacc
     1141 agaacacgac gccgaagatg cggttgttgg cctgcacgaa gatcgcctgc tcgactgcct
     1201 cgcgcagtcc cgccccccgc cccgccgcgt ccgactcgag caactccttg gcgagccgct
     1261 cggcctccgc gcggtcgccg cgcgagagcg cgtcgaggta atcgtcgact tcgtcctgca
     1321 ggtcgcgcgg cccgtacgac accagcagca cgagcacggc gaaggccagc tgcagcagcc
     1381 cgagcaggtg cgagccgatc aggtcgtacg caacggcgac cgggacgacc ggcagcaaga
     1441 cgaacagcgt caccaggatg atgagcgtcg gaccgccgag acccgccagc ctgcgcccgg
     1501 cccaatcgca gtagtggtcg agccagcgcg gctcgcgcag ccgcagcgca tcggtgagcc
     1561 cgtgctcgat caggaacgcg agcagcaggg cgatcaggct catgcgggcg ccacctccag
     1621 ctcgtaacgc gccctcgccc gcagcagcgg ccagtcgaac gcgatgccgg ggtcggactt
     1681 ccgcccgggc gcgacgtcgc tgtggccaac gacgcgctca gtcgagagtc ccttgtatgc
     1741 gcggcacagc aacgccacca gccgggccag cacctcgtac tgcgcaggtt cgtatgcgct
     1801 ggcgtcggtg ccctcgagct cgatgccgac cgagtagtcg ttgcaccgct cccgcccctg
     1861 ccagctcgac gcgcccgcat gccacgcgcg ccgctggaac ggcacgtatt gcacgacctc
     1921 gccgtcgcgc ctgacgagcg cgtgcgccga cacccgcagc ccggccacct gttcgaaata
     1981 ggggtgcgcg tcgggcggca gcgagttggt gaacagcgcg tcgatccatg gcccgccgaa
     2041 ctcgccgggt ggcaggctga tgccgtggac gacgatcagg tcgacggcga ccccctccgg
     2101 acggtcgtcg caattgggcg acgggacgcg ccgcgcgccg gaaagccacc cagtcccggc
     2161 gtcgacgtcg agttcggatc ggccggcagc ccgcgccagt gacgtgcgca tgcccccatg
     2221 atgcacgggg cccggcgtgc ccgccaccgc tccgggccga ttagaatggg tcgatccgca
     2281 gcagccggga ccaggatgga cacagcgcgc gacctcgccc aacgcgacct gagcgtcgtc
     2341 tggcacccgt gcacccagat gaaggaccac gagtggctgc cgatgatccc
//
LOCUS       DAOMFD010000275         2376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_25456_length_2376_cov_32.489444, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000275 DAOMFD010000000
VERSION     DAOMFD010000275.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2376
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25456_length_2376_cov_32.489444"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="PL152_10890"
     CDS             <1..213
                     /locus_tag="PL152_10890"
                     /inference="COORDINATES: protein motif:HMM:NF024687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49833.1"
                     /translation="LVRDISSVLADEHISIEAMNTVTNAAENIATLDLTVKVHGLEEL
                     SRLLARLAALPNVIRATRHRSPPERR"
     gene            238..2298
                     /locus_tag="PL152_10895"
     CDS             238..2298
                     /locus_tag="PL152_10895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49834.1"
                     /translation="MSATGEPIAGSTWKKLRRRKVVQWGLGYVAVAWGIVQAVAFAVG
                     TFHWPELLTRVAAVMAVAGLPLTVVLAWFHGDRGQQRVTRGELAAIVVILGLGTFAVM
                     REAREVKVANGSSERTAADHASAKLDRHRVAILPFDNLGSLAANAAFVGGVHDTLITQ
                     VAKIPGLSVISRSSVLQFAGKHPTIRDVANALGVGTVLEGSVEREGNRLRIQAQLIDA
                     GTDTHVWAETYDRTSDDLFAVQSEIAKAVAEQLRIRLTGDQGRRLLTPLSSNPEAYEH
                     YVIAREHASRFEWAAAIPELITATTLDPHFAAAYAYLSMARTWQKFVEPKSHKDNLPL
                     ALDAANKALELDPTLPEGHLAMAVYLYRGAPDIDRAAAEFERAIAGLPNDATAFQNFG
                     FLRRWQGRWEDASALFARAAQLDPKGTNPFNYPMTLVILGHRDDAAKAFAAAIAAQPH
                     DVNLALTRGWLPWVFSCDLATTERAFRDVASRFPESPDLLQAQTYLALATGDTAGAIG
                     DLARLPSAPDTSNFEASFVRALAYRDAGRRKDSDAVLRAFLQAASAEIRGMPDADVHS
                     DTLAWMAAYHAMLGDRSAAQDYVRRAIATLPPVGDASNRTDTHYFASIALAWAGDPKG
                     ALAQLEQVLGSPSWQKPAYLWCDPYLATLRKYPPFRQALAQRGADTSIDPLRRETWPK
                     PSGR"
BASE COUNT          402 a          827 c          782 g          365 t
ORIGIN      
        1 ctcgtccgcg acatctcctc cgtgctggcc gacgagcaca tcagcatcga agccatgaat
       61 acggtgacga acgccgccga gaacatcgcc accctggacc tgaccgtgaa ggtgcacggg
      121 ctcgaggaac tgagccgcct gctcgcccgg ctggcggcgc tgccgaatgt gatcagggcg
      181 acgcgccacc gctcgccgcc cgaacggcgc tagactcggc cccacgaggg ggaagcggtg
      241 tccgcgacgg gcgagccgat tgccggaagc acctggaaga agctgcggcg acgcaaggtc
      301 gtgcagtggg gcctgggcta cgtcgcggtt gcctggggca tcgtccaggc cgtcgcgttc
      361 gcggtcggca cgttccactg gccggaatta ctgacccgag tcgctgccgt catggcggta
      421 gccggcctgc cactcacggt cgtcctggca tggttccacg gcgaccgcgg ccagcagcgg
      481 gtcacgcgtg gcgagctggc ggccatcgtc gtcatcctcg ggctaggcac gttcgcggta
      541 atgcgtgaag cccgtgaggt caaggttgct aacggcagca gcgagcgcac cgccgcggac
      601 cacgcgtccg cgaaactgga ccgacaccgg gtcgccatcc tccccttcga caacctcggc
      661 agcctcgccg ccaacgccgc gttcgtgggt ggcgttcacg acaccctgat cacccaggtc
      721 gccaagatcc cgggactgtc ggtgatctcc cgcagttccg tgctgcagtt cgccggcaag
      781 cacccgacga tccgcgacgt ggcaaacgcg ctcggcgtcg gcacggtgct cgagggaagt
      841 gtcgagcgcg aagggaaccg gttgcgcatc caggcccagc tcatcgacgc cgggaccgat
      901 acccacgtat gggcggaaac ctacgaccgg acctccgacg acctgtttgc cgtgcagagc
      961 gagatcgcaa aggccgtcgc ggagcagctg cggatccgcc tgacggggga ccagggacgg
     1021 cgcctcctga cgccgctcag ctcgaacccg gaggcgtacg agcattacgt gatcgcacgc
     1081 gagcatgcca gtcgcttcga gtgggccgca gcgattcccg aactcatcac agccactacc
     1141 ctggacccgc attttgcggc ggcctacgcg tacttgagca tggcacggac ctggcagaag
     1201 ttcgtggagc cgaagtcgca caaggacaac cttccgctcg cgctggacgc cgcgaacaag
     1261 gcgctggaac tcgaccccac gctgcccgaa ggtcacctcg cgatggccgt gtacctgtac
     1321 cggggagcac ccgacatcga tcgtgcggcg gccgagttcg aacgggccat cgccggcctg
     1381 cccaacgacg cgactgcctt ccagaatttc ggcttcctga ggcgctggca agggcgttgg
     1441 gaagacgcca gcgcgctgtt tgcacgcgct gcccagctcg atccgaaggg tacgaacccg
     1501 ttcaattacc cgatgacgct ggtcatcctg ggtcacagag acgacgcggc caaggcgttc
     1561 gcggccgcga tcgcggcgca accgcacgat gtcaacctcg cgctgacgcg cgggtggctc
     1621 ccgtgggtct tctcctgcga cctggcgacg accgagcgag cgttccgcga cgtcgcgtca
     1681 cgatttccag agtcgccgga cttgctgcag gcacaaacgt atcttgcgct tgcgacggga
     1741 gacacggccg gagcgatcgg cgaccttgcc cgactcccga gcgcaccgga cacctcgaac
     1801 ttcgaggcgt cgttcgtgag ggcgctcgcg tatcgcgacg cgggtcggcg caaggactcc
     1861 gatgccgtgc ttcgggcttt cctgcaggcc gcctcggcgg agatccgcgg aatgccagac
     1921 gccgatgttc actcggacac gcttgcttgg atggctgcgt accacgcgat gctcggcgac
     1981 aggagcgccg cgcaggacta cgttcgacgc gcgatcgcga ccctgccgcc ggtcggagac
     2041 gcgtccaatc ggaccgacac gcactacttc gcctccattg cgctcgcgtg ggccggcgat
     2101 ccgaagggcg cgctggcaca gctcgagcag gtgctcggct ccccgagctg gcaaaagcct
     2161 gcatacctct ggtgcgatcc ctatcttgca acgctgcgca agtatccgcc tttcaggcag
     2221 gcgctcgccc aacgcggcgc ggacacgagc atcgacccgc tgcgccgcga gacgtggccg
     2281 aagccgtcgg gccgctgacg ttcgccagcc gccgtccacc cgtctccgga ccggcgggag
     2341 ccgctacttg ttcggcatgt cgatcgagcg cttgtc
//
LOCUS       DAOMFD010000276         2374 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_25505_length_2374_cov_32.085813, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000276 DAOMFD010000000
VERSION     DAOMFD010000276.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2374
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25505_length_2374_cov_32.085813"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..174
                     /locus_tag="PL152_10900"
     CDS             <1..174
                     /locus_tag="PL152_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004244587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HQR49835.1"
                     /translation="GFGSFSLHFRPPRQGRNPKTGESVALAGKYVPHFKPGKDLRERV
                     NEPTEVAEPATAQ"
     gene            234..524
                     /locus_tag="PL152_10905"
     CDS             234..524
                     /locus_tag="PL152_10905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HQR49836.1"
                     /translation="MLRRIATISAIVLLLAAAVLFTALNQQRFSVDFAMIRFEVSSGL
                     ALIIAFAAGLLGGALWRSHWIARLLSERGRLREALRLAESRRAESPPPQGAG"
     gene            529..1713
                     /locus_tag="PL152_10910"
     CDS             529..1713
                     /locus_tag="PL152_10910"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR49837.1"
                     /translation="MNTFWMVFTGTLATGWGATAAYAEYRRRRRARRRARYLEGVRYL
                     LDDKPERALEVFLGLAANDDETIDTHFALGSLYRRRGEVERAIRVHQHIVERTGLDPR
                     HRDAALTELARDYFRAGLYDRAEKLFLELADSGREPAVALAHLVRIHELQHDWEQAAA
                     MHERLRAVGIPDQPSAIAHYWCELAEAAIARRDLDEARAHLRTARAEHKDSGRVSILR
                     GDVARMQGDPALAVQLYRKVVRRDFHLLPLVLPRLADAARLAGDAGVFDESLRELLRN
                     GIGNRSEIAYAAIVSGYYDDPVILECVRELLSTDSDLRDLTSALLPPGTEPTMAQLRA
                     VSGALRNVVQRHARYRCSVCGIDSSTFLWQCPGCKSWDTQRAIAALEFLPRAARPGAP
                     AD"
     gene            1832..2146
                     /locus_tag="PL152_10915"
     CDS             1832..2146
                     /locus_tag="PL152_10915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HQR49838.1"
                     /translation="MKEGYRTLVRALIVSLLPAVAWGGPVNINTADAGTLARELEGIG
                     PAKAQAIVEYRQKNGPFKTPDDLLKVQGIGERVLEQNRGNIRLSSAPAAQPPKQKDTT
                     QQ"
BASE COUNT          352 a          841 c          842 g          339 t
ORIGIN      
        1 ggcttcggca gcttcagcct gcatttccgc ccgccgcgcc agggccgcaa cccgaagacc
       61 ggggagtcgg tcgcgctcgc gggcaagtac gtgccgcact tcaagcccgg caaggacctg
      121 cgcgagcggg tcaatgaacc gacggaagtg gccgagcccg cgacggccca gtgaccgggg
      181 cgcgcccggc cgggtgctag acttcccgca gcccctcacc gcccgggttg cccatgctgc
      241 ggcgcatagc cacgatttcc gccatcgtcc tgctcctcgc agcggccgtg ctgttcacgg
      301 ccctgaatca gcagcgcttc agcgtggact tcgcgatgat ccgcttcgag gtgtcctccg
      361 gcttggcgct gatcatcgca tttgcggctg gcctgctcgg cggcgccctg tggcgttcgc
      421 attggatcgc ccggctgctg tccgagcgtg gccgcctgcg cgaggccctg cggctcgcgg
      481 agtcgcgccg agcggagtcg ccgccgcccc agggcgccgg ctgattccgt gaacacgttc
      541 tggatggtgt tcaccggcac gctcgccacg gggtggggtg ccaccgcggc ctacgcggag
      601 taccggcggc ggcggcgcgc gcggcggcgc gcgcggtacc tcgagggcgt gcgctacctg
      661 ctcgacgaca agcccgagcg ggcgctcgag gtgttcctcg ggctcgccgc caacgacgac
      721 gagacgatcg acacgcactt cgcgctcggt agcctctacc gccgacgcgg cgaggtcgag
      781 cgcgcgatcc gcgtgcacca gcacatcgtg gagcggacgg ggctggatcc gcgccaccga
      841 gacgcggcgt tgaccgaact cgcgcgcgac tacttccggg ctgggctcta cgatcgcgcc
      901 gagaagctgt tcctcgagct ggccgatagc ggtcgggagc cggccgtcgc gctggcgcac
      961 ctcgtccgca tccacgagct gcagcacgac tgggaacagg ccgcggcgat gcacgagcgc
     1021 ctcagggccg tcggcatccc ggaccagccg agcgcgatcg cacactactg gtgcgaactg
     1081 gcggaggcgg cgatcgcccg tcgcgatctc gacgaagcac gcgcgcacct tcgcacggcg
     1141 cgcgccgagc acaaggactc cgggcgcgtg tccatcctgc ggggcgacgt cgcccgcatg
     1201 cagggcgatc cggcgctcgc ggtccagctc tatcgcaagg tcgtgcgccg cgacttccac
     1261 ctgctgcccc tggtgctgcc ccggcttgcg gacgccgcca ggctcgcggg cgacgccggc
     1321 gtgttcgacg agtcgctgcg cgaactgctg cgcaacggca tcgggaaccg ctcggagatc
     1381 gcctacgccg cgatcgtgag cgggtactac gacgacccgg tcatcctcga gtgcgtgcgc
     1441 gaactgctgt ccaccgacag cgacctgcgc gacctgacgt ccgcgttgct gccacccggg
     1501 accgagccca cgatggcgca gcttcgcgcg gtgtcggggg cgctgcgcaa cgtcgtccag
     1561 cgacacgcgc gttatcgctg cagcgtgtgc ggcatcgaca gctcgacctt cctctggcag
     1621 tgcccgggct gcaagtcctg ggatacgcag cgtgcgatcg ccgcgctcga attcctgccg
     1681 cgcgcggcgc ggccaggagc gcccgcggac tgagcgcatt tctgcgcgaa cacggcagga
     1741 tctgcacgcg ccggcgcgtt ctccttcggc tacggtaatc cgcgccgcac ccattccggg
     1801 cctgcggtcg acacaagcca aggaggcagt catgaaagag ggataccgga cgttggtgcg
     1861 ggcgctgatc gtcagcctgc tgccggccgt cgcgtggggc gggccggtca acatcaacac
     1921 ggcggatgcc ggcacgctgg ctcgcgaact cgagggcatt gggcccgcga aggcccaggc
     1981 gatcgtcgaa taccggcaga agaacgggcc gttcaagaca ccggatgacc tgctgaaggt
     2041 gcagggcatc ggcgagcggg tgctcgagca gaaccggggc aacatccggc tctcgtcggc
     2101 gcccgcggcc cagccgccga aacagaagga caccacccag cagtgagtgc ccgccggggc
     2161 gcggtgggcc ggtcggcgcc ggtccatcgc gccgccgtgc gtggaggcgg caaggcgtga
     2221 tccgctatga ttcgcgggct gaggggcgaa tcggcagggt tcacgcatgg cacgcatcag
     2281 gacggcggtc tttccagtcg cgggacgcgg cactcgattc cttccggcca ccaaggccag
     2341 cccgaaggag atgctgccga tcgtcgacaa gccg
//
LOCUS       DAOMFD010000277         2360 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_25781_length_2360_cov_31.087636, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000277 DAOMFD010000000
VERSION     DAOMFD010000277.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2360
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25781_length_2360_cov_31.087636"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(57..713)
                     /gene="yihA"
                     /locus_tag="PL152_10920"
     CDS             complement(57..713)
                     /gene="yihA"
                     /locus_tag="PL152_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014038472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HQR49839.1"
                     /translation="MSSYPLVKFLTSADAPRQFPPDAGGEVCIAGRSNAGKSSAINAI
                     TQRKGLARTSKSPGATRLINFFELEPGRRLVDLPGYGFAAVPGEMRQHWGQLISSYFR
                     SRGSLRGAVVVMDVRHPLTEIDCDMLELATSRALPVHVLLTKADKLGRGAAKQALAGV
                     RREVGAAVTVQLFSALSGEGLDEARRSIGRMLGSVGPKKKTPVGPADPTGEGNPALGD
                     "
     gene            793..1404
                     /locus_tag="PL152_10925"
     CDS             793..1404
                     /locus_tag="PL152_10925"
                     /inference="COORDINATES: protein motif:HMM:NF024834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HQR49840.1"
                     /translation="MTAKLAAALVVAVTVGWTTPSLAAGSKEAGQAKAATCSACHGMD
                     GTSLGPEWPNLAGQHETYIVRQLTAFHGGQRQNPLMSPMAMILTEQDMADLAAYFSSQ
                     PIRGGETDASKAKLGQRVYRSGNIDAKVMACAGCHGPTGLGNAPAAYPSLQGQKAAYV
                     ALQLRAYKAGTRTTDPNQMMRNMAAALSEEEIDAVASYVQGLR"
     gene            1437..2081
                     /locus_tag="PL152_10930"
     CDS             1437..2081
                     /locus_tag="PL152_10930"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA/DsbL"
                     /protein_id="HQR49841.1"
                     /translation="MNNKLTRFIHPLVAAGLLVLAAGTASAATVGDTQYKLLKPAQPT
                     EVAPGKVEVVEVFWYACPHCYAVQPKVEAWIKKGMPANVQYVWVPATWNELLKTHARI
                     FYTVELLGKPQLHDDVFREINVKGNRLETPEKIEAFFVTHGVSKADFQKAFSSFAVES
                     KIRHAEDLNRRYKITGTPTWVVAGKYVTEVSMAGSEDALFQVLNGLVAMEKPAG"
BASE COUNT          392 a          838 c          775 g          355 t
ORIGIN      
        1 atgagcgcat tgcgctcggc gccccgccta cctccctggg cgggccgcct gcccggtcaa
       61 tcccccaacg ccgggttgcc ttccccggtt ggatccgctg gtccaaccgg ggttttcttt
      121 ttggggccca cgctgcccag catccgcccg atcgagcgcc tcgcttcgtc caggccttcg
      181 ccgctcagcg ccgagaagag ctgaacggtc acggcggcgc caacctcccg gcgcaccccg
      241 gccagcgcct gcttcgctgc gccccggccg agcttgtcgg ccttggtgag caacacgtgc
      301 acgggcaacg cccgcgacgt ggcgagctcg agcatgtcgc agtcgatctc ggtcagtgga
      361 tgacggacgt ccatcaccac gacggcgccg cgcagcgagc cacggcttct gaaatagctg
      421 ctgatgagct gcccccagtg ctggcgcatc tcgcccggca ccgcggcgaa cccgtagcca
      481 ggcaggtcga ccagccgccg gccgggctcg agctcgaaga agttgatgag gcgggtcgca
      541 cccggcgact tgctggtgcg ggccagcccc ttgcgctgcg tgatcgcgtt gatcgcgctg
      601 gacttgcctg cattggagcg tcccgcgatg cacacctcgc ccccggcgtc cggcgggaat
      661 tgccgcggcg cgtccgcgct ggtgaggaac ttgacgagcg gatacgagga catgcgcctg
      721 tggcggtgcg gtgcgcggaa ggggtcgtag tgtacaattt acggctcaca tgcccccgtc
      781 cggagtggtg atatgaccgc gaaactcgct gccgccctcg tcgtggcggt caccgtcggc
      841 tggaccacgc cctcgctcgc cgcagggtcg aaggaggccg gccaggccaa ggccgcgacc
      901 tgcagcgcct gtcacggcat ggacggcacc agcttgggcc cggagtggcc gaacctcgcc
      961 gggcagcacg agacctacat cgtccgccag ctcacggcgt tccacggcgg ccagcgccag
     1021 aacccgctca tgtcaccgat ggccatgatc ctgaccgagc aggacatggc cgacctcgcc
     1081 gcgtacttct cgtcgcagcc gatccgcggc ggggagaccg atgcctccaa ggccaagctc
     1141 gggcagcgcg tctaccggtc cggcaacatc gacgccaagg tcatggcgtg cgccggttgc
     1201 cacgggccca ccgggctcgg caacgccccc gcagcctacc cgtcgctgca gggacagaaa
     1261 gccgcgtacg tcgcgctgca gctgcgcgcc tacaaggccg gcacgcgcac caccgacccg
     1321 aaccagatga tgcgcaacat ggccgccgcg ctctccgagg aggaaatcga cgccgtggcg
     1381 tcttatgtcc agggcctgcg ttgaaccagc ggcgtgacct gcagtcaaag ccatccatga
     1441 acaacaaact cactcgcttc atccaccctc tcgtggccgc cggcctgctc gtcctggccg
     1501 ccggcaccgc ctcggctgcc acggtcggcg acacccaata caagctgctc aagcccgcgc
     1561 agcccacgga agtcgcaccg ggcaaggtgg aggtcgtcga ggtcttctgg tacgcgtgcc
     1621 cgcactgcta cgcggtgcag cccaaggtcg aggcctggat caagaaggga atgcccgcca
     1681 acgtgcagta cgtgtgggtg cccgcgacgt ggaacgagct gctcaagacc cacgcccgca
     1741 tcttctacac ggtcgagctg ctcgggaagc cgcagctgca cgacgacgtg ttccgcgaga
     1801 tcaacgtcaa gggaaaccgg ctggagactc cggagaagat cgaagccttc ttcgtgacgc
     1861 acggcgtgtc gaaggcggat ttccagaagg cgttcagttc gttcgccgtg gaatcgaaga
     1921 tccgccacgc cgaggacctg aatcgccgct acaagatcac cggcacgccg acgtgggtgg
     1981 tcgccggcaa gtacgtcacc gaggtgtcga tggccggatc ggaagacgcg ctgttccagg
     2041 tgctgaacgg cctcgtggcg atggagaagc cggcgggctg acctccggtt cctgacacga
     2101 ccgccgtacg cagggccgcc cggcatcgcc gcgcggccct gctgttttcc cgggcactgc
     2161 gtgccggcgc tcgcgacagt gtgctaaaag ggcgcgcacc ctgggagtga ccgaccgatg
     2221 ctaaggctcc tgataccgac cggcagcggc tacgcgtggg tggaacacgc gccccccgcg
     2281 ccgttcccgg acgagttcct ctgggccgac ctgttcgagc cgacgccggc cgaggagaag
     2341 gccgtcgagt cgctgctcac
//
LOCUS       DAOMFD010000278         2323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_26551_length_2323_cov_34.345238, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000278 DAOMFD010000000
VERSION     DAOMFD010000278.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2323
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26551_length_2323_cov_34.345238"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1492)
                     /gene="speA"
                     /locus_tag="PL152_10935"
     CDS             complement(<1..1492)
                     /gene="speA"
                     /locus_tag="PL152_10935"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF003763.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008792 - arginine decarboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006527 - arginine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HQR49842.1"
                     /translation="MENTARKVAPGSHWKIEDSVELYGVDAWGNGYFAVNEAGHVVVR
                     PDQTAAHEIDLLEVIRGLEARDLTTPVVVRFSDILRHRLKRLHDAFATAITENDYRNR
                     YAAVFPIKVNQQRLVVEEVYGYGKDFGFGLEVGSKPELLAVMAMTEGESERMIICNGF
                     KDDSYIEAVILATKLGRTIIPVVENFGELQLILKHSRRYEVRPRIGVRVKLASEGAGR
                     WRESSGEKSKFGLFVTEILDLFRVLKEHGMEDCLQLVHCHPGSQLQDIRRVKDAVNEL
                     AHVYAELKLMGAGLRYIDIGGGLGVDYDGSRTNYPSSMNYTVAEYASDVVYRIASVCN
                     ARGIDHPVIVSESGRAIAAHHSLLVFNTLGSSSPEQLRLGEQAAEQLPRDASLPQPVR
                     DLLDAYRSITERRLVECYHDALQAREQALQMFSVGYLNLEARGLVERLYWATCAKIRD
                     VCRRLPSVPEELDGIESILSDIYFCNVSVFQSLPDSWAIDQLFPIMP"
     gene            complement(1577..>2323)
                     /locus_tag="PL152_10940"
     CDS             complement(1577..>2323)
                     /locus_tag="PL152_10940"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HQR49843.1"
                     /translation="RALYAAGHLPGAMYADLNADLSAPPTAASGRHPLPSPATFEAWL
                     RTRGVNRGSVVVAYDDGNAAYASRLWWMLRWVGHDEAFVLDGGLRRWTALGLPTDTRV
                     PAPGPGDFVARLRPTMVVDAAQVLASIGDEATRMLDARGPERYRGEVEPIDPVAGHVP
                     GARNHPFTTNLGDDGRFLPAAQLKAAFSEQLGQVAPRDAITMCGSGVTACQLLLAMEH
                     AGLPGARLYAGSWSEWCRDPARPVARGAAP"
BASE COUNT          354 a          790 c          823 g          356 t
ORIGIN      
        1 cgggcatgat cgggaagagc tggtcgatgg cccagctgtc gggcagcgac tggaaaaccg
       61 acacgttgca gaagtagatg tcgctcagga tcgactcgat tccgtcgagt tcctcgggga
      121 ccgacggcag gcgtcggcag acgtcgcgga tcttcgcgca ggtcgcccag tacaggcgct
      181 cgaccaggcc gcgcgcctcg aggttcaggt agccgacgct gaacatctgc agcgcctgct
      241 cgcgggcctg cagggcgtcg tggtagcact cgacgagccg ccgctcggtg atggaccggt
      301 aggcgtcgag caggtcgcga accggctgcg gcaggctggc gtcgcgcggc agctgctcgg
      361 ccgcctgttc gccgaggcgc agctgctcgg ggctcgagct gccgagcgtg ttgaacacga
      421 gcaggctgtg gtgcgccgcg atcgctcgcc ccgactcgct gacgatcacc gggtggtcga
      481 tgccacgggc gttgcacacg ctcgcaatcc ggtacacgac gtcgctcgcg tactcggcga
      541 ccgtgtagtt catggacgac gggtagttgg tgcggctgcc gtcgtagtcc acgccgaggc
      601 cgccgccgat gtcgatgtag cgcagcccgg cgcccatgag cttgagttcg gcgtacacgt
      661 gcgcgagctc gttcaccgcg tccttcacgc ggcggatgtc ctgcagctgg ctcccggggt
      721 ggcagtgcac gagctgcagg cagtcctcca tgccgtgctc cttgagcacg cggaacaggt
      781 cgaggatctc ggtgacgaac agcccgaact tggacttctc gcccgacgac tcacgccagc
      841 ggccggcgcc ctcgctggca agcttcacgc ggacgccgat gcgcggccgc acctcgtacc
      901 tgcgcgaatg cttgaggatg agctgcagct cgccgaaatt ctcgaccacc gggatgatgg
      961 tgcggccgag cttggtcgcg aggatcacgg cctcgatgta gctgtcgtcc ttgaagccgt
     1021 tgcagatgat catccgctcc gactcgccct cggtcatcgc catcaccgcg agcagctcgg
     1081 gcttggaacc tacttccagg ccgaacccaa aatccttgcc gtagccgtac acctcctcga
     1141 cgacgaggcg ctgctggttc accttgatcg ggaacaccgc ggcgtagcgg ttgcggtagt
     1201 cgttctcggt gatggccgtg gcgaacgcgt cgtgcaggcg cttcaggcgg tgacgcagga
     1261 tgtcggagaa gcgcaccacg acgggcgtcg tgaggtcgcg cgcctcgagg ccgcggatca
     1321 cttcgagcag gtcgatctcg tgggcggcgg tctggtccgg gcgcacgacc acgtggcccg
     1381 cctcattgac ggcgaagtag ccgttgcccc aggcgtcgac gccgtagagc tcgacggaat
     1441 cctcgatctt ccagtggctg ccgggagcca ccttgcgtgc cgtattttcc actcgcggct
     1501 ccgggccagg cggaaaaaag tggcgcgaac gatagcagaa gcccgccgcg gcgcaatgcg
     1561 tccgtgcgcg gcgcgatcag ggcgcggcgc cgcgggccac cggccgcgcc ggatcccggc
     1621 accactcgct ccaggagccg gcgtacaggc gtgcgcccgg aaggcccgcg tgctccatcg
     1681 cgagcagcag ctggcacgcg gtgacgccgg agccgcacat cgtgatcgca tcgcgcggtg
     1741 caacctggcc gagctgctcc gagaacgcgg ccttgagttg cgccgccggc aggaatcgtc
     1801 cgtcgtcgcc gaggttcgtc gtgaagggat ggttgcgggc gcccggcacg tgcccggcca
     1861 ccgggtcgat gggctcgacc tcgccacggt agcgctccgg accgcgggca tcgagcatcc
     1921 gcgtcgcctc gtcaccgatg ctggccagca cctgcgcggc atcgacgacc atcgtcgggc
     1981 gcagccgcgc gacgaaatcg ccgggtccgg gtgcgggcac gcgggtgtcg gtgggcagtc
     2041 cgagcgcagt ccagcggcgc agaccgccgt cgagcacgaa tgcctcgtcg tggcccaccc
     2101 agcgcagcat ccaccacagg cgtgacgcat aggccgcgtt cccgtcgtcg taggcgacca
     2161 ccaccgagcc gcggttcacg ccacgggtgc gcagccacgc ctcgaacgtg gccggcgacg
     2221 ggagcgggtg gcgcccggat gccgcggtcg gcggcgccga gagatccgcg ttcaggtccg
     2281 cgtacatcgc accggggagg tggcccgcgg cgtacagcgc ccg
//
LOCUS       DAOMFD010000279         2303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_26976_length_2303_cov_32.832295, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000279 DAOMFD010000000
VERSION     DAOMFD010000279.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26976_length_2303_cov_32.832295"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="PL152_10945"
     CDS             <1..681
                     /locus_tag="PL152_10945"
                     /inference="COORDINATES: protein motif:HMM:NF017652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HQR49844.1"
                     /translation="DQVTIAINSNQMNVVEQMCEADVAGTSLAEPANYRAYREMTIPA
                     GPEWVEEHIRRLSANGIQTHFQLASINQLETVERMMRRGVCNVPLIMTWVAIGGGFDA
                     PNIYNLANFARATPDGAVLTLETSMLNVLPLNMMAIAMGLHVRCGIEDNIWQQDRKAK
                     MSSVRQVEQLVRISREFGREVADAKEARRIYKIGTFYKNADETLAANGFVPNRKPAAA
                     RPSLRGAA"
     gene            709..1689
                     /locus_tag="PL152_10950"
     CDS             709..1689
                     /locus_tag="PL152_10950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HQR49845.1"
                     /translation="MAKAIRMHQTGGPEVLRLEDVTVGDPGPGQVRLRHEAVGLNFAD
                     TYFRTGMYPVPLPAGMGVEAAGVVEAVGDGVTNVKPGDRVTYTGFVNTLGAYSTERLI
                     PAGPLIKLPQGISCETAAAMTMRGLTSAYLMRRIWPLQAGDTILVHAAAGGVGLILTQ
                     WAKILGLTVIGTVSSKAKALVALAHGCDHVIQYGSEDVAKRVREITGGAGVAVVYDSV
                     GKDTFAGSLDSLRKRGLLVCVGTASGPIPPIDAMQLAVKGSLYVTRPALADYIGDPAE
                     KAALAGEVFGLVAAGRIRIEINQRYRLEDAVQAHRDLESRKTTGSSIFVM"
     gene            1761..>2303
                     /locus_tag="PL152_10955"
     CDS             1761..>2303
                     /locus_tag="PL152_10955"
                     /inference="COORDINATES: protein motif:HMM:NF014698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HQR49846.1"
                     /translation="MNTATTAPAVVRSTSLGSVKADAMTCAIGAELSNVNLGVASRDP
                     GLVAEIRALLLKHKVLFFRDQDITRAEHVAFARHFGELEDHPVAGSDPENPGLVRIYK
                     SPDTPIDRYENAWHTDATWREKPPMGCVLRCVECPPVGGDTMWANMAIAYERLPEHVK
                     TQIAGLRARHSIEASFGAAMP"
BASE COUNT          404 a          821 c          765 g          313 t
ORIGIN      
        1 gaccaggtga ccatcgcgat caactcgaac cagatgaacg tggtcgagca gatgtgcgag
       61 gccgacgtcg ccggcacgtc gctggccgag cccgcgaact accgcgccta tcgcgagatg
      121 accatcccgg ccggtcccga gtgggtcgag gagcacattc gccgcctgag cgccaacggc
      181 atccagacgc acttccagct cgcgagcatc aaccagctcg agaccgtcga gcgcatgatg
      241 cggcgcggcg tgtgcaacgt cccgctcatc atgacctggg tcgcgatcgg cggcggcttc
      301 gacgcgccga acatctacaa cctggcgaac ttcgcgcgcg cgacgcccga cggcgcggtg
      361 ctcacgctcg agacctcgat gctgaacgtg ctgccgctca acatgatggc gatcgcgatg
      421 ggcctgcacg tccggtgcgg catcgaggac aacatctggc agcaggaccg caaggccaag
      481 atgagcagcg tgcgccaggt cgagcagctc gtgcgcatct cgcgcgagtt cggccgcgaa
      541 gtggccgacg cgaaggaagc gcgccgcatc tacaagatcg gcacgttcta caagaacgcg
      601 gacgagaccc tggccgcgaa cggcttcgtg ccgaatcgca agccggcggc ggcgcggccc
      661 tcgctgcgcg gcgcagcctg atcgcgacca gccgcgggag ccacgcacat ggcaaaggcg
      721 atacgcatgc accagaccgg cggacccgag gtcctgcgcc tcgaggacgt cacggtcggg
      781 gacccgggtc cggggcaggt acgcctgcgc catgaggcag tgggactcaa tttcgcagac
      841 acgtacttcc gcacggggat gtacccggtc ccgctgcccg ccgggatggg cgtcgaggcg
      901 gcgggcgtgg tcgaggccgt cggcgacggc gtgacgaacg tcaagcccgg cgaccgcgtc
      961 acctacaccg gcttcgtcaa cacgctcggt gcctacagca ccgagcggct cataccggcc
     1021 ggcccgctca tcaagctgcc gcaaggcatc tcctgcgaga cggcagccgc catgacgatg
     1081 cgcggactca cctccgctta cctcatgcgc cggatctggc cgctgcaggc aggcgacacg
     1141 atcctggtgc acgccgcggc cggcggcgtc gggctgatcc tgacccagtg ggccaagatc
     1201 ctcgggctca cggtcatcgg gaccgtatcc tcgaaggcga aggcgctggt cgccctcgcg
     1261 cacggctgcg accacgtcat ccagtatggc agcgaggacg tggcgaaacg ggtccgcgag
     1321 attaccggtg gagccggcgt cgccgtcgtc tacgacagcg tcggcaagga caccttcgcg
     1381 ggctcgctcg actcgctgcg caagcgcggg ctgctggtct gcgtcggcac ggcctccggc
     1441 ccgattccgc cgatcgacgc gatgcagctg gccgtcaagg gatcgctcta cgtgacgcgc
     1501 cccgccctgg ccgactacat cggcgatcca gcggagaagg ccgcccttgc gggtgaggtg
     1561 ttcgggctcg tcgccgccgg ccggatccgc atcgagatca accagcgcta ccggctcgag
     1621 gacgcggtgc aggcgcatcg cgacctcgag tcgcgcaaga ccaccggctc ttcgatcttc
     1681 gtgatgtaac gaaccagaac acgccacatt cgcgcggccg gcccgacacg gacaccggca
     1741 acgcaccagg aggacccgac atgaacactg ccaccaccgc ccccgctgtc gtccgctcga
     1801 cgtcgctcgg gtcagtcaag gccgacgcga tgacctgcgc gatcggcgcc gaactctcca
     1861 acgtaaacct cggggtcgcg tcacgcgatc ccgggctcgt ggccgagatc cgcgccctgc
     1921 tgctcaagca caaggtcctg ttcttccgcg accaggacat cacgcgcgcc gagcacgtgg
     1981 cgttcgcccg gcatttcggc gagctggagg atcatccggt cgcaggcagc gacccggaga
     2041 atccgggtct cgtgcgcatc tacaagtcgc cggacacgcc gatcgaccgc tacgagaacg
     2101 cctggcacac cgacgccacc tggcgcgaga agccccccat gggctgcgtg ctgcgctgcg
     2161 tcgagtgccc gcccgtcggc ggcgacacga tgtgggccaa catggccatc gcctacgagc
     2221 ggctgccgga gcacgtgaag acgcagatcg ccggcctgcg cgcccgccac agcatcgagg
     2281 ccagcttcgg cgcggcgatg ccg
//
LOCUS       DAOMFD010000280         2297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_27081_length_2297_cov_34.777431, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000280 DAOMFD010000000
VERSION     DAOMFD010000280.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2297
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27081_length_2297_cov_34.777431"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(117..842)
                     /gene="yrfG"
                     /locus_tag="PL152_10960"
     CDS             complement(117..842)
                     /gene="yrfG"
                     /locus_tag="PL152_10960"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF011564.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HQR49847.1"
                     /translation="MISAPPAWSLSRNVHAEVRNPGTVRPLWAEVETVCLDMDGTVLD
                     LSFDNLFWLEALPRRWGEARGLGESESYAQLKARFDAKRGTLDWYCIDHWSEELGFDV
                     AALKSEMSGHIRYLPGATRFLDRLRELGKRMLLTTNAHPVSLGIKNRQTGLERHFHEI
                     ISSHAFGVPKESPEFWVRLALDHGVDPRTTLFVDDSVAVLTAARSAGVRWIYQVLQPD
                     STQPPHAEIAGFTGIRALTDLGP"
     gene            873..1670
                     /gene="cysQ"
                     /locus_tag="PL152_10965"
     CDS             873..1670
                     /gene="cysQ"
                     /locus_tag="PL152_10965"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HQR49848.1"
                     /translation="MASNPAELLPGVLDAVAAASTAILEVYASAHLVEYKADDSPITR
                     ADRAAHDILAARLALLAPAIPMLSEEAEAAHAHAVRGGWRELWLVDPLDGTKEFIGRN
                     GEFTVNVALIRDHEPVLGVVAAPALGVTYFAARGHGAWCTQAGGEPKPIHVRPAADPL
                     VVVGSRSHRGDSLDGLLARLGPHEMRPMGSALKFCLVAEGAADFYPRLGPTSEWDTAA
                     AQAVLEVAGGAVTALDGAPLRYNARDTLLNPHFIAYGDRTRNWPALF"
     gene            1674..1976
                     /locus_tag="PL152_10970"
     CDS             1674..1976
                     /locus_tag="PL152_10970"
                     /inference="COORDINATES: protein motif:HMM:NF023171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3301 domain-containing protein"
                     /protein_id="HQR49849.1"
                     /translation="MDLSWTLFGLLVALLAGAWYWHDSLGARERANDAALETCRGTGA
                     ALLDGTVAFRALRLVRIAGGPLQFQRTYVFDYTRDGYTREQGFVVLTGHSVDSVGL"
     gene            2074..>2297
                     /locus_tag="PL152_10975"
     CDS             2074..>2297
                     /locus_tag="PL152_10975"
                     /inference="COORDINATES: protein motif:HMM:NF016231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HQR49850.1"
                     /translation="MAEASDNVDRETFRHIERLRQLRIEHRDLDDVINRLQLDLYVDQ
                     VQMRRLKKRKLLIKDQIARLESQLIPDLNA"
BASE COUNT          350 a          815 c          799 g          333 t
ORIGIN      
        1 cggccagcga cggcggggcg ccgcgcaagc gccggcgacg aggccgtggc ggcgggcagc
       61 cgtccggcga ccggggtccc gccggcgcgc ctccggcagg gcctgcgcag gactgatcag
      121 ggcccgaggt ccgtcagcgc tcgaataccc gtgaatccgg cgatctcggc gtgcggcggc
      181 tgcgtcgagt ccggctgcag cacctggtag atccacctca cgcccgcgga gcgggccgcg
      241 gtgagcacgg cgacgctgtc gtcgacgaac agcgtggtgc gcgggtccac cccgtggtcg
      301 agcgcgagcc gcacccagaa ttccggcgat tccttcggca cgccgaacgc gtgcgacgag
      361 atgatctcgt ggaaatgacg ctccaggccg gtctgccgat tcttgatgcc gaggctgacc
      421 gggtgggcgt tggtggtgag cagcatgcgc ttgccgagtt cgcgcaatcg gtcgaggaac
      481 cgggttgcgc cgggcaggta acggatgtgg ccgctcatct cggacttgag cgccgcgacg
      541 tcgaagccga gttcctcgct ccagtggtcg atgcagtacc agtcgagcgt gccgcgcttg
      601 gcatcgaagc gcgccttcag ttgcgcgtac gactcgctct cgccgagccc gcgcgcttcg
      661 ccccagcgcc tcggcaatgc ctccagccag aacaggttgt cgaagctgag gtcgagcacc
      721 gtgccgtcca tgtcgaggca cacggtctcg acttcggccc acaggggtcg gacggtgcct
      781 ggattgcgga cttcagcgtg cacgttgcgg gagagcgacc aggccggagg ggcggatatc
      841 ataaggggtc gccatcgacc cggagccacc tcatggcctc caatcctgcc gaactgctgc
      901 ccggcgtcct cgacgccgtg gcggccgcgt ccaccgcgat cctcgaggtc tatgcgtcgg
      961 cgcacctcgt cgagtacaag gcggacgatt cgccgatcac gcgcgcggac cgcgccgcgc
     1021 acgacatcct ggcggcgcgg ctcgcactgc tcgcacccgc gatacccatg ctgtcggagg
     1081 aagccgaggc ggcccacgcg cacgccgtgc gcggcggctg gcgcgaattg tggctggtcg
     1141 acccgctcga cggcaccaag gagttcatcg gccgcaatgg cgaattcacg gtcaacgttg
     1201 cgctcatccg cgaccacgaa ccggtgctgg gagtggtggc cgcccccgcc ctcggcgtga
     1261 cctactttgc cgcgcgcggt cacggggcct ggtgcacgca ggccggcggc gaacccaagc
     1321 ccatccacgt ccgtccagcg gcggatccgc tcgtcgtcgt gggcagccgc tcgcaccggg
     1381 gcgattcgct cgacgggctg ctggctcgcc tcggaccgca cgaaatgcgc ccgatgggca
     1441 gcgcgctcaa gttctgcctc gtcgccgaag gcgccgcgga cttctacccg aggctcggcc
     1501 cgacctcgga gtgggacacg gccgcggcac aggcggtcct cgaagtcgcg ggaggagcgg
     1561 tcacggcctt ggacggtgcg ccgctcaggt acaacgcgcg cgacaccctg ctcaacccgc
     1621 acttcatcgc gtacggggac cgcacccgga actggccggc cctgttctga cgcgtggacc
     1681 tctcctggac cctgttcggc ctgctcgtcg cgctgctggc cggcgcctgg tactggcacg
     1741 acagcctggg cgcgcgcgag cgggcgaacg acgcagccct agagacctgc cgcggcacgg
     1801 gcgccgccct gctcgacggc acggtggcat tccgggcgct ccggctggtc cggatcgccg
     1861 gcggcccgct gcagttccag cggacctacg tcttcgacta cacgcgcgac ggctacacgc
     1921 gcgagcaggg cttcgtcgtg ctcaccggcc acagcgtgga cagcgtcggg ctgtgatccc
     1981 gggagatcgg ttccgccgct gggcctaccc ttttgtccgc gggcagggct aagctacccg
     2041 ccccgaccga ggccggagcg acggacacca gcgatggcgg aagcgagcga caacgtcgac
     2101 cgggagacgt tcaggcacat cgagcgcctg cgccagctca ggatcgagca ccgcgacctc
     2161 gacgacgtga tcaatcgtct gcagctcgac ctctacgtcg accaggtgca gatgcgacgc
     2221 ctgaagaagc gcaagctgct gatcaaggac cagatcgccc ggctcgagag ccagctgatc
     2281 cccgacctca acgcctg
//
LOCUS       DAOMFD010000281         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_27661_length_2270_cov_32.409932, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000281 DAOMFD010000000
VERSION     DAOMFD010000281.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27661_length_2270_cov_32.409932"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..832
                     /gene="galU"
                     /locus_tag="PL152_10980"
     CDS             <1..832
                     /gene="galU"
                     /locus_tag="PL152_10980"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014481080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HQR49851.1"
                     /translation="LPATKASPKEMLPIVDKPLIQYAAEEALQAGAERLVFVTGASKR
                     AIEDHFDTDRELERELEAQGKRDLLQAVHSIVPSYASCIYIRQPAPLGLGHAVLCSRP
                     AVGDEPFFVHLADDLIDAPEACLKQMARVFEEHEASVLGVETVKPQDTDKYGIVASDP
                     ITDRLSRVTRMVEKPKPERAPSTLAVVGRYLLTPGVYDKLERTERGAGGEIQLTDGIA
                     ALLADETVYAYAFEGRRFDCGSKLGYLQATVEFALKHTNLGPKFGAYLDDFVAHRMRR
                     "
     gene            859..2166
                     /gene="gabT"
                     /locus_tag="PL152_10985"
     CDS             859..2166
                     /gene="gabT"
                     /locus_tag="PL152_10985"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003867 - 4-aminobutyrate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009450 - gamma-aminobutyric acid catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HQR49852.1"
                     /translation="MASNQDLQKRRDAVFARGLGNQLPVYIERARNAEMWDVEGRRYI
                     DFAGGIAVLNTGHLHPKVQAAVAKQLDAFSHTCLMITPYAVAVELAEKLTRLAPGPTP
                     KKAIFVTTGAEAVENCIKIARAHTGRSGVIAFGGGFHGRTFMAMALTGKVAPYKAGFG
                     PFPAEVYHAPFPIAYHGVSVRESLEALEHLFKYDIEPARVAAIIVEPVMGEGGFYIAP
                     PEFLQALRKLCDQHGIVLVIDEIQTGFARTGRMFAIEHAGIEPDLMTMAKSLAGGFPL
                     AAVVGKAEIMDAPGPGGLGGTYAGSPIACAAGLAVIDLIQEENLCARAAAIGELMVGA
                     LRELQRELPCIGEVRNLGAMVAMELVKNRDPHAPDADLTKALTKRAADKGLVLLSCGL
                     YGNVIRFLAPLTASDAILREGLAIVADSLRELTGVPAKAAVNA"
BASE COUNT          383 a          756 c          791 g          340 t
ORIGIN      
        1 ccttccggcc accaaggcca gcccgaagga gatgctgccg atcgtcgaca agccgttgat
       61 ccagtacgcg gccgaggagg cgctccaggc aggtgcggag cgactcgtat tcgtcaccgg
      121 cgcctccaag cgagcgatcg aagaccactt cgacacggac cgggaactcg agcgggagct
      181 cgaggcccag ggcaagcgcg acctgctgca ggccgtgcac agcatcgtgc cgtcgtacgc
      241 gagctgcatc tacatccggc agccggcgcc cctgggactc ggccacgcgg tgttgtgctc
      301 gcgtccggcc gtgggcgacg agccgttctt cgtccacctg gcggacgacc tcatcgacgc
      361 ccccgaggcc tgcctcaagc agatggcccg ggtcttcgag gagcacgagg cgagcgtgct
      421 cggggtcgaa accgtcaagc cccaggacac cgacaagtac ggcatcgtgg cctcggatcc
      481 gatcacggat cgcctgtcgc gcgtgacccg gatggtcgag aagccgaagc cggagcgggc
      541 gccgtccacc ctggcggtgg tcggccggta cctgctcacc ccgggggtgt acgacaagct
      601 cgagaggacg gaacgcggcg ccggcggcga aatccagctc accgacggca tcgcggcgct
      661 gctcgccgac gagaccgtgt acgcctatgc tttcgagggt cgccgattcg attgtggatc
      721 gaagctgggg tacctgcagg cgaccgtcga gttcgcgctc aagcacacca acctcggccc
      781 gaagttcggg gcctacctcg acgatttcgt cgctcaccga atgcgccgct gacggcgcgc
      841 caacgcggag acacaacgat ggccagcaac caggacctgc agaagcgtcg tgatgcggta
      901 ttcgcccgcg gcctcggcaa ccagctgccg gtctacatcg agcgcgcgcg caatgccgag
      961 atgtgggacg tcgagggccg gcgctacatc gatttcgcgg gcggcatcgc ggtgctcaat
     1021 accggtcacc tgcacccgaa ggtgcaggcg gcggtggcga agcagctcga cgccttcagt
     1081 cacacgtgcc tgatgatcac gccttacgcg gtcgccgtgg agctggcgga gaaactgacg
     1141 cgcctcgcgc cggggccgac gcccaagaag gcgatcttcg tcaccacggg cgccgaggcc
     1201 gtcgagaact gcatcaagat cgcgcgcgcc cacacgggac gctcgggcgt gatcgcgttc
     1261 ggcggcggct tccacgggcg gacgttcatg gccatggcgc tcaccggcaa ggtcgcgccc
     1321 tacaaggccg gattcggccc gttcccggcc gaggtctatc acgcgccgtt cccgatcgcg
     1381 taccacggcg tgagcgtgcg ggagagcctc gaggccctcg agcacctctt caagtacgac
     1441 atcgagccgg ctcgggtcgc ggcgatcatc gtcgagccgg tgatgggcga gggcgggttc
     1501 tacatcgcgc cgccggaatt cctgcaagcg ctccgcaagc tctgcgacca gcacggcatc
     1561 gtgctcgtga tcgacgagat ccagaccggc ttcgcgcgta ccggccggat gttcgcgatc
     1621 gagcacgcgg gcatcgaacc cgatctcatg accatggcca agagcctcgc cggcggtttt
     1681 ccgctggcgg ccgtcgtcgg caaggcggag atcatggacg cccccggtcc gggcgggctc
     1741 ggagggacgt acgcgggttc gccgatcgcc tgcgccgcgg gcctcgcggt gatcgacttg
     1801 atccaggagg agaacctctg tgctcgggcc gcggcgatcg gggaactgat ggtcggtgcg
     1861 ctgcgcgagt tgcagcgcga actgccgtgc atcggcgagg tgcggaacct cggcgcgatg
     1921 gtcgcgatgg agctggtgaa gaaccgggat ccgcacgcgc cggatgccga tctcaccaag
     1981 gcgctcacca agcgcgcggc ggacaagggc ctcgtgctcc tgtcctgcgg tctctacggc
     2041 aacgtgatcc gcttcctggc accgctgacg gcgtcggacg ccatcctgcg cgaaggactg
     2101 gcgatcgtgg cggactcgct gcgggaactg acgggcgtgc cggccaaggc agcggtgaac
     2161 gcctgacggg acggcctgcc gcaagggttt gcggcgggtc ttcagggcga tttcggggaa
     2221 ttggctccga cacctggatt cgaaccaggg acccgctgat taacagtcag
//
LOCUS       DAOMFD010000282         2263 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_27818_length_2263_cov_30.516304, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000282 DAOMFD010000000
VERSION     DAOMFD010000282.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27818_length_2263_cov_30.516304"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /gene="mnmA"
                     /locus_tag="PL152_10990"
     CDS             <1..390
                     /gene="mnmA"
                     /locus_tag="PL152_10990"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF015042.2"
                     /note="catalyzes a sulfuration reaction to synthesize
                     2-thiouridine at the U34 position of tRNAs; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HQR49853.1"
                     /translation="GQRQGLEVGGVRGAAEAPWYVAAKDLPRNVLVVVQQHDHPLLMS
                     NEIETEPAHWVADAPPAGRFDCTLKTRYRQADQDCTVEIRPDGGCVVHTRLPQRAVTP
                     GQSAVFYAGPVCLGGAVIARSRYNSAA"
     gene            420..>2263
                     /gene="acnA"
                     /locus_tag="PL152_10995"
     CDS             420..>2263
                     /gene="acnA"
                     /locus_tag="PL152_10995"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HQR49854.1"
                     /translation="MKDSFKTRQSLAVGSRSYEIHSLPAIKGHDLGRLPYSLKILLEN
                     LLRFEDGVNVTRADIEALLKWDAKAAPSHEIAFTPARVIMQDFTGVPGIVDLAAMREA
                     MVKLGGNAQDINPLAPVELVIDHSVQVDNYGTADALRKNNEIDFARNGERYSFLRWGQ
                     SAFRNFKVVPPSTGIVHQVNVEHLARVVFAEERDGKQVAYPDTVVGTDSHTTMVNGLG
                     VLGWGVGGIEAEAAMLGQPVTMLIPHVIGFRLKGSLPAGATATDLVLTITEMLRKKGV
                     VDKFVEFFGDGLASLPLADRATIANMSPEFGSTCGIFPVDEETIRYLELTGRPAEQVA
                     LVEAYAKAQGLWRTNGMREADYTDVLELDLAMVVPSLAGPKRPQDRVPLSVSKETYRK
                     HVATMAAERATKNPSATGKASVTSGGKTFELVDGAVVIAAITSCTNTSNPNVLIAAGL
                     LARKAHQRGLAAKPWVKTSLAPGSRVVTDYLKKAGLLAELESVGFYVVGYGCTTCIGN
                     SGPLRPEISEGIKAGDIVATSVLSGNRNFEGRVHAEVKMNFLASPPLVVAYALAGTMD
                     IDMNRDPIGTGSDGKPVYLKDLWPSPQEVQQTVAASLDAGMFKKSYSSV"
BASE COUNT          390 a          783 c          748 g          342 t
ORIGIN      
        1 gggcagcggc agggcctcga ggtcggcggc gtgcgtggcg cggccgaggc gccgtggtac
       61 gtcgccgcga aggatctccc gcgcaacgtg ctcgtcgtcg tgcagcagca cgaccaccct
      121 ctgctcatga gcaacgaaat cgagacggag ccggcgcact gggtggcgga tgcaccgccg
      181 gccggccgat tcgattgcac gctcaagacg cgctatcgcc aggcggacca ggactgtacg
      241 gtggagatcc ggccggatgg cggctgcgtc gtgcacacgc ggctcccgca gcgcgccgtg
      301 acgcccggcc agtccgccgt gttctacgct ggcccggtct gcctcggggg ggcagtcatc
      361 gcccggagcc gttataattc cgcggcttag tcgggcccgt tcgctttccc ggaggtcgca
      421 tgaaggacag tttcaagacc cgccagtcgc tggcggtggg cagccgttcc tacgagatcc
      481 acagcctgcc cgcgatcaag ggccacgacc tcgggcgcct gccctactcg ctcaagatcc
      541 tgctcgagaa cctgctgcgc ttcgaggacg gcgtcaacgt cacgcgcgcc gacatcgagg
      601 cgctgctcaa gtgggacgcc aaggcggccc cgagccacga gatcgccttc acgccggcgc
      661 gcgtgatcat gcaggacttc accggcgtgc ccggcatcgt cgacctcgcc gccatgcgcg
      721 aggcgatggt gaagctcggc ggcaacgcgc aggacatcaa tccgctcgcc ccggtcgagc
      781 tcgtgatcga ccactccgtg caggtggaca actacgggac cgccgacgcc ctcaggaaga
      841 acaacgagat cgacttcgcg cgcaacggcg agcgttactc gttcctgcgc tggggccagt
      901 ccgccttccg caatttcaag gtcgtgccgc ccagcacggg catcgtccac caggtcaacg
      961 tcgagcacct cgcgcgagtg gtgttcgcgg aagagcggga cggcaagcag gtcgcatacc
     1021 cggacaccgt ggtcggcacc gattcgcaca cgacgatggt gaatggcctc ggcgtcctcg
     1081 gctggggcgt cggcggcatc gaggccgagg ccgccatgct cggccagccg gtcacgatgc
     1141 tcatcccgca cgtcatcggc ttccgcctca agggctcgct tccggccgga gcgaccgcca
     1201 cggatctcgt gctcacgatc accgagatgc tgcgcaagaa gggcgtggtg gacaagttcg
     1261 tcgagttctt cggcgacggc ctcgccagcc tgccgctggc cgaccgggcc acgatcgcca
     1321 acatgtcgcc cgagttcggc agcacgtgcg gcatcttccc cgtggacgag gagacgattc
     1381 gctatctcga actcaccggg cgcccggccg agcaggtcgc gctggtggag gcctacgcca
     1441 aggcgcaggg cctgtggcgc acgaacggca tgcgcgaggc ggactacacc gacgtgctcg
     1501 aactcgacct cgcgatggtc gtaccgagcc tcgccggccc caagcggccg caggaccggg
     1561 tcccgctcag tgtgtccaag gagacctatc gcaagcacgt cgcaacgatg gccgccgagc
     1621 gcgcgacgaa gaatccttcg gcgacgggca aggcctcggt cacgagcggc ggaaagacct
     1681 tcgaactcgt ggacggcgcc gtggtgatcg cggcgatcac cagctgcacg aacacgtcga
     1741 atccgaatgt actgatcgct gccggcttgc tcgcacgcaa ggcacatcag cgcggcctcg
     1801 ccgccaagcc gtgggtcaag accagcctgg cgcccggttc gcgcgtggtc accgattacc
     1861 tgaagaaggc cggcctgctc gccgaactgg aatccgtcgg cttctacgtc gtgggctacg
     1921 gttgcacgac atgcatcggc aactcgggcc cgctgcgccc ggaaatctcg gaggggatca
     1981 aggccggcga catcgtcgcg acctcggtac tgtcgggcaa tcgcaacttc gagggacgcg
     2041 tccacgccga ggtgaagatg aatttcctcg cctcgcctcc gctcgtggtc gcctacgcgc
     2101 tcgccggcac catggacatc gacatgaacc gcgacccgat cggcaccggc tccgacggca
     2161 agcctgtgta cctcaaggat ctctggccgt caccgcagga ggtccagcag accgtcgctg
     2221 catcgctcga cgcgggcatg ttcaagaaga gctactcgag cgt
//
LOCUS       DAOMFD010000283         2245 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_28218_length_2245_cov_33.360274, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000283 DAOMFD010000000
VERSION     DAOMFD010000283.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28218_length_2245_cov_33.360274"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1707
                     /gene="xsc"
                     /locus_tag="PL152_11000"
     CDS             <1..1707
                     /gene="xsc"
                     /locus_tag="PL152_11000"
                     /EC_number="2.3.3.15"
                     /inference="COORDINATES: protein motif:HMM:NF005713.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050487 - sulfoacetaldehyde
                     acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0019529 - taurine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoacetaldehyde acetyltransferase"
                     /protein_id="HQR49855.1"
                     /translation="LVANGVTDVFGIVGSAYMDALDIFPAAGIRFIPVAHEQGAAHMA
                     DGYARASGRHGVCIAQNGPGITNFVTAIAAAYWAHSPVVFITPETGSLTMGLGGFQET
                     DQLPIFSKITKYQGHVASPRRMAEIAARCFDRAMLEMGPCQLNIPRDHFYGDIQAVIP
                     PPTRIGRGPGDASALDEAAALLATARFPVIVAGGGVIHAGARDEAIRLAETLGAPVCT
                     SYLHNDAFPASHALWCGPLGYQGSKAAMKLIAQADVVLALGTRLGPFGTLPQYGMEYW
                     PAGAKIIQVDADAKVLGLVKPISVGIHGDARAAAAALIERLQGRELACVANRSQRNTK
                     VRAEKDAWEQELTAWTHERDPYSLEVAKDSRFMHPRQMLRELERAMPADAMVSTDIGN
                     ICSVSNSYLRFDRPRSMFAAMSFGNCGYAFPVACGAKVASPDRPAVAYVGDGAFGISL
                     NELLTCAREKIGVTVVVFNNGQWGAEKKNHVDFYSRRFLAVDLENPSWAGIADAFGCN
                     GVTVDRLEDVGPALRAAVAAQADGQTTVLEMMVTRELGDPFRRDALSKPVRYLDKYRT
                     YT"
     gene            1751..>2245
                     /locus_tag="PL152_11005"
     CDS             1751..>2245
                     /locus_tag="PL152_11005"
                     /inference="COORDINATES: protein motif:HMM:NF018028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HQR49856.1"
                     /translation="MPIDEARFEYVGFWARLLASLVDSVLVCMLVAPLLTLAYGSGYW
                     EPIVALWRDRGDLSALLAAADTATGAESVAGGGPVEFIVVWVLPAIAFIAFWLAREAT
                     PGKMLVGARIVDAETGGPITRRQAIGRYLGYYVSLLGGFLGFFWVGWDRRKQGWHDKL
                     AGTVV"
BASE COUNT          335 a          762 c          783 g          365 t
ORIGIN      
        1 ctcgtcgcga acggcgtgac cgacgtgttc ggcatcgtgg gctcggcgta catggatgcg
       61 ttggacattt ttcctgccgc tggcatccgc ttcatcccgg tcgcgcacga gcagggcgcg
      121 gcgcacatgg cggacggata cgcccgcgct tccggccgcc atggcgtctg cattgcgcag
      181 aacggccccg gcatcaccaa cttcgtcacg gcgatcgcgg cggcctactg ggcgcattct
      241 cccgtcgtgt tcatcacgcc cgagacgggc tcgctcacga tgggactcgg cgggttccag
      301 gagaccgacc agctgccgat cttctcgaag atcaccaagt accagggaca cgtggccagc
      361 ccgaggcgga tggccgagat cgcggcccgc tgcttcgacc gtgcgatgct cgagatgggt
      421 ccgtgccagc tcaacatccc gcgtgaccat ttctacggcg acatccaggc cgtgattccg
      481 ccgcccacgc gcatcgggcg cggaccggga gatgcgtccg ccctcgacga ggcggccgcg
      541 ctgctcgcga ccgcgcggtt cccggtcatc gttgccggcg ggggagtcat ccacgccggc
      601 gccagggatg aagcgatccg cctggcagag acgctgggcg ccccggtgtg cacgagttac
      661 ctgcacaacg atgcgttccc ggcgtcgcac gcgctgtggt gcggtccgct cggctaccag
      721 ggatcgaagg ccgcgatgaa gctcatcgcg caggccgacg tcgtgctggc cctgggaacg
      781 cgcctcggcc cgttcggcac cctgccgcaa tacggcatgg agtactggcc ggccggcgcg
      841 aagatcatcc aggtcgatgc cgacgccaag gtgctcggcc tggtgaagcc aatcagcgtg
      901 ggtatccacg gcgatgcgcg cgcggcggcc gcagcactga tcgagcggct gcagggtcgc
      961 gaactcgcct gcgtggccaa ccgcagccag cgcaacacga aggtccgcgc agagaaagac
     1021 gcctgggagc aggaactcac tgcgtggact cacgagcgcg acccgtattc gctcgaggtc
     1081 gcgaaggact cgaggttcat gcacccgcgg cagatgctcc gcgagctcga gcgcgcgatg
     1141 cccgccgacg cgatggtgtc caccgacatc ggcaacatct gctcggtgtc gaactcctac
     1201 ctgcggttcg accgcccgcg ctcgatgttc gcggccatga gcttcggcaa ctgcggctac
     1261 gccttcccgg tcgcctgcgg cgccaaggtc gcgagtccgg accggccggc agtcgcctac
     1321 gtcggcgacg gcgcattcgg gatcagcctg aacgagttgc tcacctgcgc gcgggagaag
     1381 attggcgtga cggtggtcgt cttcaacaac ggccagtggg gcgccgagaa gaagaaccac
     1441 gtggacttct attcacgccg gttcctggcc gtggacctcg agaacccgag ctgggccggc
     1501 atcgcggacg cgttcggctg caacggggtg accgtggatc gcctggagga cgtcgggccc
     1561 gccttgcggg ctgcggtcgc ggcgcaggcc gacgggcaga cgacggtgct cgagatgatg
     1621 gtcactcgcg agctgggcga tccgttccgt cgcgacgcgc tctcgaagcc ggtccgctac
     1681 ctcgacaagt accgcaccta cacctagtct caccgggtcc gccgctgtcg gcggcgcacg
     1741 gagaggtcgc atgccgatcg acgaggcacg gttcgagtac gtcgggttct gggcacgcct
     1801 gctcgcgtcg ctggtcgact cggtgctggt ctgcatgctc gtcgcgccgc tgctcacgct
     1861 ggcctacggc agcggctact gggaaccgat cgttgcgctc tggcgcgacc gtggcgacct
     1921 ctcggcactg ctggccgccg ccgacaccgc cacgggcgcc gagtccgtgg ccggcggcgg
     1981 tccggtggaa ttcatcgtcg tctgggtgct gccggcgatc gcgttcatcg cattctggct
     2041 tgcacgcgag gcgacgcccg gcaagatgct ggtcggcgcg cggatcgtgg atgcggagac
     2101 gggtggaccc atcacgcgcc ggcaggcgat cggtcggtac ctcggttact acgtctcgct
     2161 gctcggcggc ttcctcggct tcttctgggt cggctgggac cggcgcaagc agggctggca
     2221 cgacaagctc gcgggcacgg tcgtc
//
LOCUS       DAOMFD010000284         2244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_28243_length_2244_cov_31.938785, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000284 DAOMFD010000000
VERSION     DAOMFD010000284.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28243_length_2244_cov_31.938785"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="PL152_11010"
     CDS             <1..590
                     /locus_tag="PL152_11010"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HQR49857.1"
                     /translation="QVTTALPPIFQVSLEKLPPLELPPALEREPGPTSAPETPQGPPR
                     KTRPVPILVDTPVANPVPPGTQVKQYEMGDELMEGEPGPPSPPGAVVGGTGDTAGTQQ
                     LPVIEKVALERMKPPVYPPRCLRMGIEGLVKVRVLVGEDGRPQEITLAKSSGESSLDE
                     AAMDAVKDWIFKPAQRNGLPIRAWATVPIEFKLID"
     gene            887..1345
                     /locus_tag="PL152_11015"
     CDS             887..1345
                     /locus_tag="PL152_11015"
                     /inference="COORDINATES: protein motif:HMM:NF013760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HQR49858.1"
                     /translation="MQHHDQLTEDRLAGTLHRADFLDRALRASINREMERLETGLTFL
                     ATVGSTAPFVGLFGTVWGIFHALVKIGATGETSLDQVAGPVGESLIMTALGLAVAVPA
                     VLGYNLLVRNNRVIIASFHAFAEDMLAYLTTGARVPSSMDHHRTHMKRAA"
     gene            1364..1801
                     /locus_tag="PL152_11020"
     CDS             1364..1801
                     /locus_tag="PL152_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005411739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HQR49859.1"
                     /translation="MAFSGLGGSGQPHTTPMAEINTTPLVDVMLVLLIIFMITAPLLA
                     HKIKIDLPQASTIPLEEKPDTIVIALLPDGTMFLNDARIDRESLVRRLASEAQRKPQP
                     ELHLHVDRTTQFQVLATIMADAQNAGMVKVGIATEPLKDRKQP"
     gene            1817..>2244
                     /locus_tag="PL152_11025"
     CDS             1817..>2244
                     /locus_tag="PL152_11025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HQR49860.1"
                     /translation="MPLTVSLEISDQELEHFRTVLRSARTKAARRQPHEIASAASAAV
                     EKLRSAAPSPFVAKRLQKVDALIRMLDDPEWRLPHAERCRVLDGLAYVAEIHDLVPDD
                     VPVLGLVDDAIMLELVLRELQHELESYGEFDDYRRQEHLEV"
BASE COUNT          395 a          788 c          733 g          328 t
ORIGIN      
        1 cgcaggtgac gacggcgctg ccgccgatat tccaggtgag cctggagaag ctcccgccgc
       61 tcgaactccc tccggccctg gaacgggaac cgggcccgac gagcgcgccg gaaacgccgc
      121 agggaccacc gcgcaagacg cggcccgtcc cgattctcgt cgacacccct gttgcgaatc
      181 cggttccacc gggcacccag gtcaagcagt acgaaatggg cgacgaactc atggagggcg
      241 aacccggtcc gccgagcccg cccggcgcgg tggtcggcgg gacgggcgat acggctggca
      301 cgcagcaact gccggtcatc gagaaggtcg cgctggagcg gatgaagccg ccggtctacc
      361 cgccgcggtg cctgcgcatg ggcatcgaag gcctggtgaa ggtccgcgtg ctcgtcggcg
      421 aggacggccg accccaggag atcacgctgg cgaagtcgag cggcgagtcg tcgctcgacg
      481 aggcggcgat ggacgccgtg aaggactgga tcttcaagcc cgcgcaacgt aacgggctgc
      541 caatacgagc ctgggccacg gtgcccatcg aattcaagtt gattgactga cggtcgagga
      601 gcgagacatg catcccaccg aatcgctgac gacgatgagc ttcgccaact tcctccggca
      661 gacggacgcc gtcgggctgt tcgtcttcgg gctgctggtg ctcatgtcga tcgtctcctg
      721 gtacttcatg atcatcaagt tcctgcgcgt cgcgcgcatc cggacgcggt cgcaccacgt
      781 cgtctcctcg ttctggaaga cgccgcccga ccaggccatc gccttcctcg agcagcagcc
      841 ggactgggag ccgttctcga aaatcgccct cgatgccgag ttcgcgatgc agcaccacga
      901 ccagctgacc gaggaccggc tcgccggaac gctgcatcgg gcggacttcc tcgaccgcgc
      961 gctgcgcgcc tcgatcaacc gcgagatgga acggctggag accggtctca ccttcctcgc
     1021 gaccgtcggc agcaccgcgc cgttcgtcgg cctgttcggc acggtctggg ggatcttcca
     1081 cgcactggtg aagatcggcg cgacgggcga gacctccctc gaccaggtgg cgggcccggt
     1141 gggtgaatcg ctgatcatga cggcgctggg cctcgcggtc gcggtgccgg cggtgctcgg
     1201 ctacaacctg ctcgtgcgca acaaccgcgt gatcatcgcg agcttccacg cattcgcgga
     1261 agacatgctc gcgtacctca cgaccggggc gcgcgtcccg tcgtccatgg accatcaccg
     1321 cacccacatg aagcgtgcgg cctgagcgcg aacggagcca gccatggcat tcagcggact
     1381 cggcggcagc ggccagcctc acacgacccc gatggcggag atcaacacca cgccgctggt
     1441 ggacgtgatg ctggtgctgc tcatcatttt catgatcacg gcgccgttgc tggcgcacaa
     1501 gatcaagatc gacctgccgc aggcctcgac gatcccgctg gaggaaaagc ccgacacgat
     1561 cgtgatcgcg ctgttgcccg acggcacgat gttcctcaac gacgcgcgca tcgaccgcga
     1621 gagcctcgtg cggcggcttg cgtccgaggc gcagcgcaag ccgcaaccgg agctgcacct
     1681 gcacgtggac cgcacgaccc agttccaggt cctcgcgacg atcatggcgg acgcgcagaa
     1741 cgccggcatg gtcaaggtgg gcatcgcgac cgagccgctc aaggaccgaa agcagccgta
     1801 gcgcgggaga gccggcatgc ccctgaccgt ctcgctcgag atctccgacc aggagctcga
     1861 acactttcgc accgtcctgc ggagcgcccg caccaaggcg gcgcgccggc agccgcacga
     1921 gatcgcctcc gcggcaagtg ccgccgtcga aaagctgcgc tccgccgcgc cctcgccctt
     1981 cgtcgcgaag agactgcaga aagtcgacgc cctgatccgc atgctcgacg accccgagtg
     2041 gcggctgccc cacgccgagc gctgccgggt cctcgacggc ctcgcctacg tcgccgagat
     2101 ccacgacctc gtgcccgacg acgtgcccgt gctggggctc gtggacgacg ccatcatgct
     2161 cgaactggtg ctgcgcgaac tgcagcacga gctcgagagc tacggcgagt tcgacgacta
     2221 ccgccggcag gagcacctcg aggt
//
LOCUS       DAOMFD010000285         2241 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_28297_length_2241_cov_34.322964, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000285 DAOMFD010000000
VERSION     DAOMFD010000285.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2241)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2241
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28297_length_2241_cov_34.322964"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="PL152_11030"
     CDS             <1..426
                     /locus_tag="PL152_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HQR49861.1"
                     /translation="WMPFVPHCELVSIKLFRADPVRGEVISVLRAPPGVELPRYRHAG
                     SATIYTVQGRWKYLEHDWVAGPGSVVIETAASRHTPQVMPDGTDDVIMFIVTEGELLL
                     LDEDGNVVASENWRTAVDRYLEYCRANDIEPRDVTGSSA"
     gene            complement(442..1938)
                     /locus_tag="PL152_11035"
     CDS             complement(442..1938)
                     /locus_tag="PL152_11035"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HQR49862.1"
                     /translation="MGLRDYGDYDALGLADLVRTGQVSATELLDEALARTAAVNPRIN
                     AVIHLMEGRARDAIAAGLPDGPFRGVPFLIKDLMTAYAGEPMTSASRLFRDYVPAADE
                     ELTRRYRRAGLVIFGKTNTPEFGATNVTEPELFGPARNPWNLERTPSGSSGGSAAAVA
                     ARIVPAANANDGGGSIRTPASNCGLVGLKPSRGRVPSGPLAPDLWFGFIAEHAVTRTV
                     RDCAALLDATAGDYPQQLMKLPPASGPYLDEVGRDPGRLRIAYTFDPGLATSLHPENR
                     AGLEATVAALTKSGHDLVEVHLPLPSDQFIASYALLVSADIAATLRYAKQLVGREARR
                     EDVELSNWILARIGEAHRAVEVSAAYWEAQAFSRRWLEWFSAFDVLLTPTVGVPPIPV
                     GANRLSRSRRLGLRLLAALPDGMVRRQQPKVIEAFRSTFEIAPYTMVANVTGQPSMSL
                     PLHWTADGLPMGMLFTARTGDEATLFRLAAQLEQVMPWKERRAPHAAA"
     gene            1941..>2241
                     /locus_tag="PL152_11040"
     CDS             1941..>2241
                     /locus_tag="PL152_11040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49863.1"
                     /translation="MSLRLDVIESMIAGPRGEIDRRLYVAAALADCTRASRRTCDGER
                     LRHVALQTLLPAAAGRPTLDASTRVQDRHMMRSRAAMLTIAIVCLAGLPGAEPSLA"
BASE COUNT          343 a          791 c          767 g          340 t
ORIGIN      
        1 tggatgccgt tcgtgccgca ttgcgagctc gtctcgatca agctcttccg ggcggacccg
       61 gtgcgcggcg aagtcatcag cgtgctgcgc gcgcccccgg gcgtggagct gccgcgctac
      121 cgccacgccg gttcggcgac gatctacacg gtgcagggcc gctggaagta cctcgagcac
      181 gactgggtcg cgggacccgg cagcgtcgtg atcgagaccg cggcgtcgcg ccacaccccg
      241 caggtgatgc cggacggcac cgacgacgtg atcatgttca tcgtcaccga aggcgagttg
      301 ctgctgctcg acgaggacgg caacgtggtc gcctccgaga actggcgcac cgccgtggat
      361 cgctacctcg agtactgccg tgcgaacgac atcgagccgc gcgacgtgac gggatcgagc
      421 gcctgaggat ccgcgccgac gctacgccgc cgcgtgcggg gcccgccgct ccttccacgg
      481 catcacctgc tcgagctgcg ccgcgagacg aaagagcgtc gcctcgtctc ccgtgcgcgc
      541 cgtgaacagc atgcccatcg gcagcccgtc cgccgtccag tgcagcggca gcgacatcga
      601 gggctgtccc gtcacgttcg cgaccatggt gtagggtgcg atctcgaacg tgctgcggaa
      661 cgcctcgatc accttcggct gctggcgacg caccatgccg tcgggcagcg cggcgagcag
      721 gcgcagtccc aggcgccgcg accgcgacag gcggttcgcg ccgaccggga tgggcggcac
      781 gcccacggtc ggcgtcagca gcacgtcgaa tgccgagaac cactcgagcc accgccgcga
      841 aaaagcctgc gcctcccagt acgcggcact cacctcgacg gcccggtgcg cctcgccgat
      901 gcgcgccagg atccagttcg acagttccac gtcctcgcgc ctcgcctcgc gaccgaccag
      961 ctgcttcgca tagcgcagcg tcgccgcgat gtcggcggac acgagcagcg cgtagctcgc
     1021 aatgaactgg tccgacggca agggcaggtg gacctcgacg aggtcgtgac cagacttcgt
     1081 cagtgccgct accgtagctt cgagcccagc ccggttctcc gggtgcaacg aagttgcgag
     1141 gcctgggtcg aacgtgtacg caatgcgcag gcggccggga tcccgcccca cttcgtcgag
     1201 gtacggaccc gacgctggcg gcagcttcat gagttgctgc ggatagtcgc ccgcagtcgc
     1261 atcgagcagc gccgcgcagt cgcgcaccgt gcgggtgacg gcgtgttccg cgatgaatcc
     1321 gaaccacagg tccggcgcga gaggtccgct cggtaccctg ccccggctcg gcttcaggcc
     1381 gacgaggccg cagttcgagg ccggcgtgcg tatcgagccg ccgccatcgt tggcattggc
     1441 ggccggcacg atgcgcgccg cgaccgcggc cgccgagccg ccgctcgagc cgctcggcgt
     1501 gcgctcgagg ttccacggat tgcgcgccgg gccgaagagc tccggctccg tgacgttggt
     1561 cgcgccgaat tccggcgtgt tggtcttgcc gaagatgacc aggcccgcgc gccggtagcg
     1621 tcgcgtgagt tcctcgtccg cggccggcac gtagtcgcgg aacaggcgcg aagcgctggt
     1681 catcggctcg ccggcatacg cggtcatcag gtccttgatg aggaacggga cgccgcggaa
     1741 cggaccgtcg ggcagccccg ccgcgatggc gtcgcgcgca cggccttcca tcaggtggat
     1801 gactgcgttg atcctgggat tgacggccgc cgtcctcgcc agcgcctcgt cgaggagttc
     1861 cgtggcgctc acctgcccgg tccgcaccag gtcggccagg ccgagcgcgt cgtaatcgcc
     1921 gtagtcgcgc aatcccatgg atgtcgctcc gtctcgatgt gatcgagagc atgatcgccg
     1981 gtccgcgcgg ggaaatcgat cgtcgcttgt acgtggccgc agcactcgcc gactgcacac
     2041 gtgcgtcacg aagaacgtgc gacggtgaac gcctgcggca tgttgcactg caaacgctct
     2101 tgccagcggc ggcgggccga ccgacactcg acgcgtccac acgcgtacag gaccgacaca
     2161 tgatgcgtag ccgtgccgcg atgctgacca tcgcgatcgt ctgtctcgcc gggcttccag
     2221 gtgccgagcc gtcgctcgcg a
//
LOCUS       DAOMFD010000286         2235 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_28425_length_2235_cov_36.932110, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000286 DAOMFD010000000
VERSION     DAOMFD010000286.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28425_length_2235_cov_36.932110"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..1169
                     /locus_tag="PL152_11045"
     CDS             171..1169
                     /locus_tag="PL152_11045"
                     /inference="COORDINATES: protein motif:HMM:NF024039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HQR49864.1"
                     /translation="MLNKSTALAMTAAPLKEAVQQYGLDFAELARRAGIDPELLSRAN
                     ARYPAEGLQRLWELSAAASGDPLFGIRVGNLIRPGMFHALGLGIVSSTSVLGSLKRIE
                     RYSSVVSTNGRMVVVGHDGTVGLETRPNETTIMPTTQSVDAIVVGLCRLLEQCAGPSA
                     IPAKVVFMRPRVAPAEAYRAVLKCPVEFDGNHVGMLFDAVAVEQPVLTGNAELAAEAD
                     RLATRYIEGLVPDSTAAKVRALLLKAMPSGEFDQDVIAKSLNQSPSTLQRRLRKEGTT
                     YQGLLDATRRDLAFDYLRQGRDSLADIAFLLGFTDQANFTRAFRRWTGRTPREFLS"
     gene            complement(1201..2109)
                     /locus_tag="PL152_11050"
     CDS             complement(1201..2109)
                     /locus_tag="PL152_11050"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HQR49865.1"
                     /translation="MHASSLRGVVAMLVAVAVFSGMDAQLKLLAAHYGPMQVAFIRGL
                     TSLPFVLLPVLLRGRVARLRIVNLRLHLVRGGIAVLMLSAFIYAVRGSSLANTYSIFM
                     CAPLVVAALSAPMLGERVAGAQWVAIAVGLAGVLLMLRPSGGDWVSLASLAAVFSALC
                     YAFAAITMRLLARTDTTESMVFWFTVILTVGAGLLSIPAWRPFEPADWPLCVGVGVCG
                     AIAQHFITEAFRHAPVSVVTPLEYTALVWGVVLDVAIWHVLPAATTLAGGAIVVGAGL
                     YVIRREALQRTRAAGPRAAARRDGTG"
BASE COUNT          386 a          762 c          780 g          307 t
ORIGIN      
        1 cggcgcaacc ttgtcttatc gtccatgggg cagcactcta ggcaggcgag cggctggcct
       61 cttgtcatcg gacggcaacg tcttgtccgg gcaggtcagg gggccgcctg gatgaacata
      121 ttgccggcca gccgccaaca tcgggcgggt ccggctggct cgagacggcg atgctcaaca
      181 agtcgactgc gctggcgatg accgcggccc cgctgaagga ggcggtgcag caatacggcc
      241 tggatttcgc ggaactcgcg cggcgggcgg gcatcgaccc tgagttgctc tcgcgtgcca
      301 atgcgcgtta cccggccgag ggcctgcagc gactctggga actctcggcc gctgcgtcgg
      361 gcgatccgct gttcggaatt cgcgtcggca acctcatccg accgggcatg tttcacgcgc
      421 tcgggctcgg catcgtgagc agcacgagcg tcctcgggtc gctcaagcgc atcgaacgct
      481 acagcagcgt cgtcagtacc aacggacgca tggtcgtcgt agggcacgac ggcacggtcg
      541 gcctcgagac ccggcccaac gagaccacga tcatgccgac gacgcagagc gtggacgcga
      601 tcgtcgtcgg tctgtgccgc ttgctggagc agtgcgcggg accgtcggcc attccggcga
      661 aagtcgtctt catgcgtccg cgagtcgcac cggccgaggc gtaccgcgct gtgctgaaat
      721 gcccggtcga gttcgacggc aatcacgtcg ggatgctgtt cgacgccgtg gccgtggaac
      781 agcccgtgct gaccgggaat gcggaacttg cggcggaagc ggaccggctg gcgacgcgtt
      841 acatcgaagg cctggtgcct gactcgacgg cggccaaggt tcgggcgctg ctgctgaaag
      901 cgatgccgtc gggggagttc gaccaggacg tcatcgcgaa gtcgctgaac cagagcccga
      961 gcacgctgca gcggcgcctg cgcaaggaag ggacgacgta ccagggcctg ctcgacgcga
     1021 cgcgacgcga cctggcgttc gactacctgc ggcaggggcg cgacagcctc gccgacatcg
     1081 ccttcctgct gggattcacc gaccaggcca atttcacgcg cgcattccgc cgctggacgg
     1141 ggcgtacgcc gcgcgaattc ctctcctgac cggcgcggcc gtggcgatgc gcgggcccga
     1201 tcacccggtg ccgtcgcgac gagcggcggc tcgcggccct gcggctcgcg tgcgctgaag
     1261 cgcctcgcgc cggatgacgt acaacccggc accgaccacg atcgcgccac cggccagcgt
     1321 cgtcgctgcc ggcagcacgt gccagatcgc gacgtcgagc acgacgcccc agacgagcgc
     1381 ggtgtattcg agcggcgtaa ccaccgacac cggcgcgtga cggaaggctt ccgtgatgaa
     1441 gtgctgggcg atcgcgccgc agacgccgac acccacgcac agcggccagt cggccggctc
     1501 gaacggccgc cacgccggaa tggacagcag tcccgctccc acggtgagga tcaccgtgaa
     1561 ccagaacacc atgctctccg tggtgtcggt gcgcgcgagc agccgcatgg tgatcgccgc
     1621 gaacgcgtag cagagtgccg agaacaccgc cgcgaggctt gccagcgaca cccagtcgcc
     1681 gccggagggg cgcagcatga gcagcacgcc cgcgaggccg acggcgatgg ccacccactg
     1741 ggcgccggcg acccgctcgc ccagcatcgg cgcggacaac gcggccacga ccagtggcgc
     1801 gcacatgaag atcgagtagg tgttggcgag cgacgagccg cggaccgcgt agatgaacgc
     1861 cgacagcatc agcaccgcga tcccgccacg gaccaggtgc aggcgcaggt tgacgatcct
     1921 caggcgcgcg acgcgcccgc gcagcaggac cggcagcagc acgaacggca gcgaagtgag
     1981 gccacggatg aaggcgacct gcatcgggcc gtaatgcgcg gccagcagct tgagctgggc
     2041 gtccatgccg gagaagacgg ccaccgcgac cagcatcgcg accacacccc tcaggctgct
     2101 cgcgtgcacg ggatcggctc cctcggggag tgaatggcct cgccggcggc taccggccga
     2161 cggctcagcg cttcatggcg tcgaagaact cggcgttggt cttggtgtcc cgcatcttgt
     2221 cgatgaggaa ctccg
//
LOCUS       DAOMFD010000287         2222 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_28720_length_2222_cov_44.923858, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000287 DAOMFD010000000
VERSION     DAOMFD010000287.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2222
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28720_length_2222_cov_44.923858"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="PL152_11055"
     CDS             <1..678
                     /locus_tag="PL152_11055"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HQR49866.1"
                     /translation="WDSAVVFGDVDGAPGSEAVAMLSSNSGGSGELVYVAVFGTRDGK
                     LVNLATAMVGDRTKLQNLWLQQGHVVMDVVEAGPKDPACCPTQLARKTYALEGGALKL
                     AKNEVLGTLTMGTLAANEWQLISIDGKPLPEGAKPPVVHFEPKDVRGFAGCNRFTAPI
                     KETSPGVIVVGPAAATRMSCPEPAMQLEQEFLQSLEKTSGYTFLAGRLALTRSGKEGN
                     GTMLFSK"
     gene            complement(745..1914)
                     /locus_tag="PL152_11060"
     CDS             complement(745..1914)
                     /locus_tag="PL152_11060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HQR49867.1"
                     /translation="MLSDRALDSLPSPAARRVRDVFDAMKIATRVENKKVLVGLAPSV
                     ARGFLLKEGKQGEPVGMRASHDAYFDFRYQGDYPEMYDLYRRAVQNQWDGDRQLDWST
                     DVDPLHPERPVFPLELAPMEGLAAYGIRLTRDEQLRFVADFSSWMLSQFMHGEQGALY
                     ASAQVTESVHWVDGKFYGATQVMDEARHLEVFLRYLESKLGKLYQVNDNLFTILDALM
                     RDSRWDMKFLGMQIMIEGLALGAFGTMYQATREPLLKEMLRYVIQDEARHVHYGVLAL
                     KRHFAELSPAELREREDWTFEVACLMRDRFLAHEIYDEWFAGIMKRSAWDEVISSSPF
                     LQQFRQIMFRRLVPNLEYIGIFSPRIRSHYEKVGLTQYAGGRNASQLTAEDLLAA"
     gene            complement(1956..>2222)
                     /locus_tag="PL152_11065"
     CDS             complement(1956..>2222)
                     /locus_tag="PL152_11065"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster binding
                     domain-containing protein"
                     /protein_id="HQR49868.1"
                     /translation="HAAHITFAASGKRVTARPGQTVLQAGLEAGVDLPYSCTMGGCGA
                     CKVHKSSGSVVASEPNCLTDSEREAGYVLACCSYADTDLTIDNQ"
BASE COUNT          355 a          731 c          774 g          362 t
ORIGIN      
        1 tgggacagcg cggtcgtatt cggtgacgtc gacggtgcgc cgggcagcga agccgtcgcg
       61 atgctgagtt ccaacagcgg cggcagcggt gaactcgtct acgtcgcggt gttcggcacg
      121 cgcgacggga agctcgtgaa cctcgccacg gcgatggtcg gcgaccgcac caagctgcag
      181 aacctctggc tgcagcaggg ccacgtcgtg atggacgtcg tggaggctgg cccgaaggat
      241 ccggcctgct gcccgaccca gctcgctcgc aagacttatg cgctcgaggg cggggcgctc
      301 aagctcgcga agaacgaggt gctcggcacg ctcacgatgg gaacgctcgc cgccaacgag
      361 tggcagctga tctccatcga cggcaagccg ctgcccgagg gcgcgaagcc gcccgtcgtg
      421 cacttcgagc caaaggacgt gcgcgggttc gcaggctgca atcggttcac cgcgccaatc
      481 aaggagacgt caccgggcgt gatcgtggtg ggtccggccg cggccacacg catgtcctgc
      541 ccggagcccg cgatgcagct ggagcaggag tttctccagt cattggagaa gacgtctggc
      601 tacacgttcc tcgccggccg cctcgcgctc acccggtccg gcaaggaagg caacgggacg
      661 atgctgttct cgaagtagtg cgacgcacgg gtgccggcgg cggcaggcgc gcgccgcagg
      721 ccgccagccc gtcgtggaac agcatcacgc cgcgagcagg tcctccgccg tcagttgcga
      781 cgcattgcgc cctccggcgt actgcgtgag cccgaccttc tcgtagtgcg agcggatgcg
      841 cggcgagaag atgccgatgt actcgaggtt cggcaccagt cgccggaaca tgatctgccg
      901 gaactgctgc aggaagggcg aggacgagat cacctcgtcc caggccgagc gcttcatgat
      961 cccggcgaac cactcgtcgt agatctcgtg cgcgaggaag cggtcgcgca tcaggcaggc
     1021 gacctcgaac gtccagtcct cgcgctcgcg cagctccgcc gggctcagct ccgcgaagtg
     1081 ccgcttgagc gccagcacgc cgtagtgcac gtggcgcgcc tcgtcctgga tgacgtagcg
     1141 cagcatttcc ttgagcagcg gctcgcgcgt ggcctggtac atggtgccga aggcgccgag
     1201 cgcgaggccc tcgatcatga tctgcatgcc gaggaacttc atgtcccagc gcgagtcgcg
     1261 catcagcgcg tcgaggatgg tgaacaggtt gtcgttcacc tggtagagct tgccgagctt
     1321 gctctcgagg tagcgcagga acacctcgag gtggcgcgcc tcgtccatca cctgggtcgc
     1381 gccgtagaac ttgccgtcga cccagtgcac ggactcggtc acctgcgccg aggcgtagag
     1441 ggcaccttgt tcgccgtgca tgaactggct cagcatccag gatgagaagt cggcgacgaa
     1501 gcgcaactgc tcgtcacgcg tcaggcggat gccgtacgcg gcgaggcctt ccatcggcgc
     1561 gagttcgagc gggaacaccg gtcgctcggg gtggagcggg tcgacgtccg tcgaccagtc
     1621 gagctgcctg tcgccgtccc actggttctg caccgcgcgg cggtacaggt cgtacatctc
     1681 cgggtagtcg ccctggtagc ggaagtcgaa gtacgcgtcg tgcgacgcac gcatccccac
     1741 cggctcaccc tgcttgcctt ccttgagcag gaagccgcgc gccaccgagg gcgcgaggcc
     1801 gaccaggacc ttcttgttct cgacgcgcgt cgcgatcttc atcgcgtcga agacgtcgcg
     1861 gacccggcgg gcggccgggc tgggcaggga atcgagggcg cgatccgaaa gcatgtgtgt
     1921 ctccgtggtg ccgaacggcg ctgggccgcg gcgtgtcatt ggttgtcgat ggtcaggtcg
     1981 gtgtcggcgt agctgcagca ggcgaggacg tagcccgcct cccgctcgct gtccgtaagg
     2041 cagttcggct cgctggcgac gaccgagccg ctcgacttgt gcactttgca ggctccacag
     2101 ccgcccatcg tgcagctgta gggcaggtcg acgcccgcct ccagccccgc ttgcagcacg
     2161 gtctggcccg ggcgcgccgt cacgcgcttg ccgcttgccg cgaaggtgat gtgcgcggcg
     2221 tg
//
LOCUS       DAOMFD010000288         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_29656_length_2181_cov_37.919567, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000288 DAOMFD010000000
VERSION     DAOMFD010000288.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29656_length_2181_cov_37.919567"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="PL152_11070"
     CDS             <1..1020
                     /locus_tag="PL152_11070"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR49869.1"
                     /translation="LIVGIPLMQILLFGYAINFDVRNLRAAVVDEAGTSLSRALVADL
                     RATGVIALRAPSPSVAELRRRINAGEISVGIVIPADFERRRLESDRAAVQILVDGSEP
                     MVDSIARSLASTPIPGRHGLRPAGGRTYEIYTEYNPERRTAVQVVPALIGVILNMTMV
                     IFTSAAIVRERERGNLELLITTPIRSGELMTGKLLPYVFIGLLQATLVLLVGMWLFDV
                     PLNGSLVQFYLAAGLFIASTLTLGLVISTVAQTQLQAFQMAFMTMLPSILMSGFVFPF
                     DGMPKPAQWFAQLLPLTHFVDMVRGVILRGAPLADLKVQALKLAVFFVVTLAIATRRF
                     RKRLD"
     gene            complement(1085..>2181)
                     /locus_tag="PL152_11075"
     CDS             complement(1085..>2181)
                     /locus_tag="PL152_11075"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR49870.1"
                     /translation="IQYSPQTPNVYAEPVLVVPAWIMKYYILDLSPKNSMVNWLTSLG
                     HTVFMISWKNPTADDRDITMDDYIEHGVLAALDAVTTVVPKHKVHAVGYCIGGTLLAI
                     AAALMAAREDERLASVTIFAGQTDFSEPGEISIFISPAQLAMLEAMMWKSGVLESRQM
                     GGAFQMLRTYDLLWAPTVGTYLRGERASLNDLMAWNADGTRMAYRMHTDYLHQLYLGN
                     DLAEGRYVAMGEPIQLSDITVPMFVVGTETDHVAPWRSVYKAGRLVRSRDYTFCLTSG
                     GHNAGIISGPQHPKRRHRILQTRAGTRLTPPDKYLDRAEMLQGSWWPTWAKWLEERSG
                     AHKVKPPAMGAPKKGLKPICDAPGTYVKQS"
BASE COUNT          304 a          752 c          742 g          383 t
ORIGIN      
        1 ttgatcgtgg gcatcccgct catgcagatc ctgctgttcg ggtatgcgat caacttcgac
       61 gtgcgcaacc tgcgcgcggc ggtcgtggac gaggcgggca cgagcctgtc gcgggcgctg
      121 gtcgccgacc tccgggcgac cggcgtgatc gcgctgcggg cgccgagccc cagcgtcgcg
      181 gagctgcgcc gccgcatcaa tgccggcgag atcagcgtcg gcatcgtgat cccggccgat
      241 ttcgaacggc ggcggctcga gtcggaccgc gcggccgtgc agatcctcgt ggacggcagc
      301 gaaccgatgg tcgacagcat cgcgcgttcc ctcgcctcga cgccgattcc cggtcgccac
      361 ggactgcggc cggccggcgg gcgcacctac gagatctaca ccgagtacaa ccccgagcga
      421 cgcaccgcgg tgcaggtcgt gcccgcgctg atcggcgtga tcctgaacat gaccatggtg
      481 atcttcacct cggcggcgat cgtccgcgag cgcgagcgcg gcaacctgga gctgctcatc
      541 accacgccga tccgttccgg cgagctgatg accggcaagc tgctgccgta cgtcttcatc
      601 ggcctgctgc aggcgaccct ggtgctgctg gtcggcatgt ggctgttcga cgtgccgctg
      661 aacggcagcc tcgtgcagtt ctacctggcg gcggggctgt tcatcgcttc gacgctgacg
      721 ctgggcctgg tgatctcgac cgtggcgcag acccagctgc aggccttcca gatggccttc
      781 atgaccatgc tgccgtcgat cctgatgtcc gggttcgtgt tcccgttcga cggcatgccc
      841 aagccggcgc agtggttcgc gcagctgctg ccgctcacgc acttcgtgga catggtgcgc
      901 ggcgtgatcc tgcgcggcgc gccgctcgcg gacctcaagg tgcaggcgtt gaagctcgcc
      961 gtgttcttcg tcgtgacgct cgccatcgcc acgcggcggt tccgcaagcg gctggactga
     1021 gaccgcggcc agcggccagg cgcgccgccc cggccccgga gtccggcact gcgaccgccg
     1081 ctggtcagct ctgcttcacg taggtccccg gcgcgtcgca gatcggcttc aagcccttct
     1141 tcggcgcgcc catcgcgggc ggcttgacct tgtgggcacc cgagcgctcc tcgagccact
     1201 tcgcccacgt cggccaccac gagccctgca gcatctcggc tctgtcgagg tacttgtccg
     1261 gcggtgtcag gcgcgtgccc gcccgcgtct gaaggattcg atgccggcgc ttcgggtgct
     1321 gcgggccgct gatgatgccg gcgttgtgcc cgccgctcgt caggcagaac gtgtagtcgc
     1381 gcgaccgcac cagccggccg gccttgtaca ccgagcgcca cggcgcgacg tgatcggtct
     1441 cggtgccgac gacgaacatc ggcacggtga tgtccgacag ctggatcggc tcgcccatgg
     1501 cgacgtaccg gccttccgcg aggtcgttcc cgaggtacag ctggtgcagg tagtcggtgt
     1561 gcatgcggta ggccatgcgc gtgccgtcgg cgttccaggc catcaggtcg ttcaggctgg
     1621 cacgctcgcc gcgcaggtag gtgcccaccg tcggcgccca cagcaggtcg taggtgcgca
     1681 gcatctggaa cgcgccgccc atctgccggc tctcgagcac gcccgacttc cacatcatcg
     1741 cctcgagcat cgcgagctga gccgggctga tgaagatgga gatctcgccg ggctcgctga
     1801 agtcggtctg gcccgcgaag atcgtcacgc tcgcaaggcg ctcgtcctcg cgagcggcca
     1861 tcagggccgc cgcgatggcg agcagcgtgc ccccgatgca atagccgacc gcgtgcacct
     1921 tgtgcttcgg cacgacggtc gtcaccgcgt cgagcgcagc cagcacgccg tgctcgatgt
     1981 agtcgtccat ggtgatgtcg cggtcgtcgg cggtcgggtt cttccacgag atcatgaaca
     2041 cggtgtggcc gaggctcgtg agccagttga ccatcgagtt cttcggcgac aggtcgagga
     2101 tgtagtactt catgatccac gccggcacga cgagcaccgg ctcggcgtag acgttcggcg
     2161 tctgcggcga gtactggatc a
//
LOCUS       DAOMFD010000289         2181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_29657_length_2181_cov_36.954374, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000289 DAOMFD010000000
VERSION     DAOMFD010000289.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29657_length_2181_cov_36.954374"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1278
                     /gene="purD"
                     /locus_tag="PL152_11080"
     CDS             <1..1278
                     /gene="purD"
                     /locus_tag="PL152_11080"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020651200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HQR49871.1"
                     /translation="MKVLIIGSGGREHALAWKAAQSPRVTQVYVAPGNAGTAFEPRVR
                     NVEVDADDVPGLLRFALAEKIDLTIVGPEAPLVLGVTDEFSAAGLRCFGPSRLAARLE
                     GSKAFMKEFLRRHSIPTAAYATFTRGTFDAAWVRAQRAPLVVKADGLAAGKGVVICAT
                     VDEAVATAKDMFAGRFGTAGSTIVVEEFLEGEEASFIAMVDGTHVLPLATSQDHKRRD
                     DGDRGPNTGGMGAYSPAPVVTPEVHERAMREVMWPTVRALASEGTPYVGFLYAGLMIA
                     ADGAPRVLEFNCRLGDPETQPILSRLRSDITLHCEAAIDGRLATEHADWDPRAAVGVV
                     LAAGGYPDSVRKGDVIHGLDAAARLPGKVFHAGTALRDGSVVTNGGRVLCAVGLGDSV
                     RIAQQQAYDLARAISWDGMQYRRDIGYRAIARE"
     gene            complement(1279..>2181)
                     /locus_tag="PL152_11085"
     CDS             complement(1279..>2181)
                     /locus_tag="PL152_11085"
                     /inference="COORDINATES: protein motif:HMM:NF026213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase dimerization domain-containing
                     protein"
                     /protein_id="HQR49872.1"
                     /translation="RDVYANLNNEVLHLANDGVTINEVQNVYRLPPSLKTRWEAHSYH
                     GSEEHNSRAVINRYLGYWDGNPATLIPLSPQDSAPLYVEMMGGSQKIMAKGRQLYDSG
                     QYLLATEILNKLVYAEPGNQAAKDLLADCFEQIGYQKESSSLRNSFLAAALELRSGIP
                     GGITPKTATPDVVRAMSTGLWLDYLGILLDADKAEGMHFVINIVTPDNGEKYAVELSN
                     STLTNIEGFAAPHPDLTVTVNQADLQRVMMQQATFASLAGEGKAKLEGNAQVFQQLMG
                     CMTQFDPAFEIMPGTKAAAPAPAR"
BASE COUNT          323 a          734 c          750 g          374 t
ORIGIN      
        1 atgaaagtac tcatcatcgg ttccggcggc cgcgagcacg cgctcgcgtg gaaagcggcg
       61 caatcgccgc gcgtgacgca ggtgtacgtg gcgccgggca atgcgggcac ggcattcgag
      121 ccgcgcgttc gcaatgtcga ggtcgacgct gatgacgtcc ccgggctgct gcgctttgcg
      181 ctggcggaaa agatcgacct gaccatcgtc ggccccgagg ccccgctggt gctcggtgtc
      241 accgacgagt tctccgccgc gggactgcgc tgcttcggcc cgagtcggct ggcggcgcgc
      301 ctcgaaggct cgaaggcctt catgaaggaa ttcctgcgcc ggcattcgat cccgaccgcc
      361 gcgtacgcga cgttcacgcg cgggacgttc gacgccgcgt gggtgcgcgc tcagcgcgct
      421 cccctcgtgg tcaaggccga cggtctcgcg gccggcaagg gcgtggtcat ctgcgccacg
      481 gtcgacgagg ccgtcgcgac cgcgaaggac atgttcgccg ggcggttcgg gacggcgggt
      541 tcgaccatcg tcgtcgagga attcctcgag ggcgaggaag ccagcttcat cgcgatggtg
      601 gacggcacgc acgtgctgcc gctcgcgacg tcccaggatc acaagcgccg cgacgacggc
      661 gaccgcggcc cgaacacggg cggcatggga gcatattcgc cggcgccggt ggtgacaccc
      721 gaggtgcacg agcgcgcgat gcgcgaggtc atgtggccga cggtgcgtgc gctcgcatcg
      781 gagggaaccc cgtacgtagg cttcctgtac gccggcctga tgatcgctgc cgacggcgct
      841 ccccgcgtgc tcgagttcaa ctgccggctc ggtgacccgg agacgcagcc catcctgtcg
      901 cggctgcgct cggacatcac ccttcactgc gaggccgcaa tcgacgggcg cctcgcgacg
      961 gaacacgcgg actgggaccc gcgcgctgcc gtgggtgtcg tgctggccgc cggcggctat
     1021 ccggacagtg tgcgcaaagg tgacgtgatc cacggcctcg acgccgcagc ccggctgcct
     1081 ggcaaggtct tccacgcggg caccgccctg cgtgacggct ccgtcgtgac gaacggcggc
     1141 cgcgtgctct gcgccgtcgg gctcggcgac agcgtcagga tcgcgcagca gcaggcctac
     1201 gacctggccc gggccatctc ctgggacgga atgcagtacc gcagggacat cggctaccgg
     1261 gcgatcgctc gcgagtagct accgcgccgg cgccggtgcc gcagccttcg tccccggcat
     1321 gatctcgaac gccgggtcga actgggtcat gcagcccatc aactgctgga acacctgtgc
     1381 attgccctcg agcttcgcct tgccctcgcc cgcgagcgac gcgaaggtcg cctgctgcat
     1441 catcacgcgc tggaggtccg cctggttcac ggtgaccgtg aggtcggggt gcggcgctgc
     1501 gaagccctcg atgttggtca gcgtcgagtt gctcagctcg accgcatact tctcgccgtt
     1561 gtcgggcgtg acgatgttga tgacgaagtg catcccctcc gccttgtcgg cgtcgagcag
     1621 gatgccgagg tagtcgagcc acaggcccgt cgacatcgcg cggacgacgt ccggcgtcgc
     1681 ggtcttcggc gtgatgccac ccggtatgcc cgacctcagc tcgagcgccg cggcgaggaa
     1741 gctgttgcgc aggctcgaac tttccttctg gtaaccgatc tgctcgaaac agtcggccag
     1801 caggtccttg gccgcctggt tgccgggctc ggcgtacacg agcttgttca aaatctccgt
     1861 cgcaagcagg tactggcctg aatcgtagag ctgccggccc ttcgccatga tcttctgcga
     1921 accgcccatc atctccacgt agagcggcgc cgagtcctgc ggcgacaacg ggatcagcgt
     1981 cgccggattg ccgtcccagt aacccaggta gcgattgatg acggcgcggc tgttgtgctc
     2041 ctcggagccg tggtagctgt gcgcttccca ccgtgtcttc aggctcgggg gcagacgata
     2101 gacgttctgc acctcgttga tggtgacgcc gtcgttcgcg aggtgcagga cctcgttgtt
     2161 caggttcgcg tacacgtcgc g
//
LOCUS       DAOMFD010000290         2161 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_30181_length_2161_cov_36.381766, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000290 DAOMFD010000000
VERSION     DAOMFD010000290.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2161)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2161
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30181_length_2161_cov_36.381766"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1424)
                     /locus_tag="PL152_11090"
     CDS             complement(<1..1424)
                     /locus_tag="PL152_11090"
                     /inference="COORDINATES: protein motif:HMM:NF023169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMIN domain-containing protein"
                     /protein_id="HQR49873.1"
                     /translation="MTAASFNTIEPREGARQSHGRRRPAALVALAGLAAFLAQAVFAQ
                     DALRLEKVDVQPQPGQQVEVRLVLNGPAPQPVTFTVDNPARLSLDLPGASLGLPSRRV
                     DVNAGGVDTIVAAEASGRVRLVFNMDRMLPYQTRVSGDTVFVTIGGSGPTQAASVPAP
                     ASGGGKAASTAAAVQKVDFRRGTDGAGRVLVTTSSSNVQASLKQEGGRIVIDFPNTTV
                     PQEYLKRFDVVDFATPISSFDVTQGPNGARIVVAAAGDYDQLAYQSDRDYVLEVRQRS
                     KAAVVDPSKREYKGERLTLNFQDIETRAVLQLLAETSGQNIVVSDSVQGNVTLRLQNV
                     PWDQALDIVLRTKGLDKRQDGNVIYVAPSEELQAREKQQLEAQKAIVELAPVRTEYLQ
                     VNYAKASDLAALIKSQGKTSLLSDRGSVSVDERTNTLLLQDTADRLADIRRLVSTLDI
                     PVKQVLIESRIVIVNDSFSRELGVR"
     gene            complement(1444..1953)
                     /locus_tag="PL152_11095"
     CDS             complement(1444..1953)
                     /locus_tag="PL152_11095"
                     /inference="COORDINATES: protein motif:HMM:NF016254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilP"
                     /protein_id="HQR49874.1"
                     /translation="MTTALRIVLAAAILATAAGCSDDTEQLRKQIAEIKSRPGERIQP
                     LPEVKPYETFAYPDADQRSPFEPGVPASASAPGAIRPDANRPREFLEQFSLDTLKMVG
                     TLKLQGRVFGLVQTKDGLVHRVLPGNHLGQNDGRITAIDEGKISLIEIVPDGMGGFIE
                     RPAALALSD"
     gene            complement(1982..>2161)
                     /gene="pilO"
                     /locus_tag="PL152_11100"
     CDS             complement(1982..>2161)
                     /gene="pilO"
                     /locus_tag="PL152_11100"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HQR49875.1"
                     /translation="RIVTLHDIEIVPANKDSGNAGDLVLNVTAKTYRYLDEEEQAADD
                     KAGGKKGGKPPAQAK"
BASE COUNT          277 a          742 c          763 g          379 t
ORIGIN      
        1 cgcacgccca gctcgcggct gaagctgtcg ttcacgatca cgatgcgcga ctcgatcagc
       61 acctgcttga ccgggatgtc gagcgtcgag accaggcggc ggatgtccgc cagccggtcg
      121 gcggtgtcct gcagcaggag cgtgttggtg cgctcgtcga ccgacacgct gccgcggtcg
      181 gacagcagcg aggtcttgcc ctgcgacttg atcagcgccg cgaggtcgga ggccttcgcg
      241 tagttgacct gcaggtactc ggtgcggacc ggggcgagtt ccacgatcgc cttctgcgcc
      301 tcgagctgct gcttctcgcg cgcctgcagt tcctcggacg gcgcgacgta gatgacgttg
      361 ccgtcctggc gcttgtcgag gcccttggtc ctgagcacga tgtcgagcgc ctggtcccac
      421 ggcacgttct gcagccgcag ggtgacgttg ccctgcaccg agtcgctcac cacgatgttc
      481 tggccgctcg tctcggcgag cagctgcagc accgcgcggg tctcgatgtc ctggaagttg
      541 agcgtcagcc gctcgccctt gtattcgcgc ttgctcgggt cgaccaccgc cgccttcgac
      601 ctctggcgga cttcgagcac gtagtcgcgg tcgctctggt aggcgagctg gtcgtagtcg
      661 ccggccgcgg cgaccacgat gcgggcgccg ttcgggccct gcgtcacgtc gaacgagctg
      721 atcggcgtgg cgaagtccac cacgtcgaag cgcttcaggt actcctgggg cacggtcgtg
      781 ttcgggaaat cgatgacgat gcggccgcct tcctgcttga ggctcgcctg cacgttggag
      841 ctgctggtcg tcacgaggac gcggccggcg ccatccgtgc cccggcggaa gtccaccttc
      901 tgcacggccg ccgccgtgct cgcggccttg ccgccgccgg atgccggcgc cggtacggac
      961 gcagcctgtg tcgggccgct cccgccgatg gtcacgaaga cggtgtcgcc ggacacgcgc
     1021 gtctggtagg gcagcatccg gtccatgttg aacacgaggc gcacgcggcc ggaggcctcg
     1081 gccgccacga tggtgtcgac gccgccggcg ttgacgtcca cccggcgcga aggcagcccg
     1141 agcgaggccc cggggaggtc gagcgacagc cgcgcggggt tgtcgaccgt gaaagtgacc
     1201 ggctgcggcg ccggaccgtt caggacgagg cgcacctcga cctgctggcc gggctgcggc
     1261 tggacgtcca ccttctcgag gcgcaacgca tcctgcgcga agacggcctg cgcaaggaac
     1321 gcggcgaggc ccgccagcgc gacgagcgcc gcgggcctgc gccggccgtg cgactggcgc
     1381 gcgccttcgc ggggttcgat cgtgttgaac gatgcagcgg tcatgctcga gtctccttgc
     1441 agatcagtcg ctcagcgcaa gcgcggcggg gcgttcgatg aagccgccca tgccgtcggg
     1501 gacgatctcg atgagcgaga tcttgccttc gtcaatggcc gtgatacggc cgtcgttctg
     1561 gcccaggtgg ttgccgggta gcacgcggtg cacgagcccg tccttcgtct gcaccagccc
     1621 gaagacgcgg ccctgcagct tcagcgtccc gaccatcttc agcgtgtcca gcgagaactg
     1681 ctcgaggaac tcgcgcgggc ggttggcgtc gggccggatc gcgcccgggg cgctcgccga
     1741 cgccggcacg cccggctcga acggcgagcg ctggtccgcg tcggggtagg cgaaggtctc
     1801 gtacggcttg acctcgggca gcggctggat gcgctcgccc ggccggctct tgatctccgc
     1861 gatctgcttg cgcagctgct cggtgtcgtc cgagcatccc gccgccgtcg cgaggatggc
     1921 cgccgccagg acgatgcgca gtgctgtggt catgttcgct cgcgcctgtg ccatcgctgc
     1981 ctcacttggc ctgggcgggc ggcttgccgc ccttcttgcc gcccgccttg tcgtccgccg
     2041 cctgctcctc ctcgtcgagg tagcgatagg tcttggcggt cacgttcagc acgagatcac
     2101 ccgcgttacc cgagtccttg ttggccggga cgatctcgat gtcgtgcagc gtgacgatgc
     2161 g
//
LOCUS       DAOMFD010000291         2125 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_31107_length_2125_cov_30.604348, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000291 DAOMFD010000000
VERSION     DAOMFD010000291.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31107_length_2125_cov_30.604348"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /gene="zwf"
                     /locus_tag="PL152_11105"
     CDS             <1..1200
                     /gene="zwf"
                     /locus_tag="PL152_11105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HQR49876.1"
                     /translation="DSLEKHGGIDEAAFAKLCSLLRYVDGDYADPATFAALRKTLGGA
                     QRPAHYLAIPPVLFGKVVEQLAASGCSSGARVVVEKPFGRDLESARKLNAILHATFDE
                     EHVFRIDHYLGKRPVHNMVFFRFANAFLESFWNRKFVKSVQITMAESFGVQGRGAFYD
                     TVGTVRDVVQNHLFQVLANLCMEPPARADSESLRDEKVKVLKSIPPIAESDLVRGRFR
                     GYLDEPGVAKDSRTETFAALRLEIDSWRWAGVPFYIRAGKSLPVTCTEVLVRLRRAPR
                     IFPGCTGVPNHLRFRISPEMTIALGATVMDIDDKSIGQQMELTASRRPGPEEMDAYER
                     VLGDAMEGDRTLFAREDYVEEAWRIVDPVLKAGTPVHEYEPGTWGPPEVSAVAPPGGW
                     ADPVVQA"
     gene            1204..1956
                     /gene="pgl"
                     /locus_tag="PL152_11110"
     CDS             1204..1956
                     /gene="pgl"
                     /locus_tag="PL152_11110"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HQR49877.1"
                     /translation="MSAVQVEVFDDAQAVAEAAAGYIAGQARVAVAARGRFLLAVSGG
                     RTPWVMLAALATRPLPWSAIEVFQVDERVAPAGDPDRNLVHLREALLQHAPLQPEQVH
                     AMPVEAADLTRAARDYAAILESCSGVPPVLDLVHLGLGPDGHTASLVPGDPSLEVSDA
                     DVTLSGPYQGHRRMTLTYPVIDRARRVMWVVTGAEKAAMLRRLLDGDRAIPAGRVCRD
                     RAIAFVDRDAAGDVERAQFPGSNGAVPARGNA"
BASE COUNT          313 a          714 c          766 g          332 t
ORIGIN      
        1 gacagcctcg agaagcacgg cggcatcgac gaggcggcgt tcgcgaagct ctgctccctg
       61 ctgcgctacg tcgacggtga ttacgccgac ccggccacct tcgccgcgct gcgcaagacc
      121 ctgggtggcg cgcagcggcc cgcgcactac ctcgcgatcc cgccggtgct cttcggcaag
      181 gtcgtggagc agctcgcggc atccggctgc agttccggtg cccgcgtcgt cgtcgagaag
      241 ccgttcgggc gcgatctcga gtcggcgcgc aagctgaacg cgatcctcca tgccaccttc
      301 gacgaggagc acgtcttccg catcgaccac tacctcggca agcggccggt gcacaacatg
      361 gtgttcttcc gcttcgcgaa cgcgttcctc gagtcgttct ggaaccggaa gttcgtgaag
      421 agcgtgcaga tcacgatggc ggagagcttc ggcgtgcagg ggcgcggggc gttctacgat
      481 accgtcggca ccgtgcgcga cgtcgtccag aatcacctgt tccaggtcct cgccaacctg
      541 tgcatggagc cgccggcacg cgcggacagc gagtcgctgc gcgacgagaa ggtgaaggtc
      601 ctgaagtcca tcccgccgat cgcggagagc gacctcgtgc gcggccggtt tcgcggctac
      661 ctggacgagc ccggtgtcgc gaaggactcg cgcaccgaga ccttcgccgc gctgcgtctc
      721 gagatcgact cctggcgctg ggccggcgtg ccgttctaca tccgcgcagg caagtcgctg
      781 ccggtgacgt gcaccgaggt gctcgtgcgg ctgcggcgcg cacccaggat ctttccgggt
      841 tgcaccggtg tgccgaacca tcttcgcttt cgtatcagcc ccgagatgac gatcgccctc
      901 ggggcgacgg tgatggacat cgacgacaag tcgatcggcc agcagatgga gctgacggcg
      961 agccgccgtc cggggcccga agagatggac gcctacgagc gcgtgctcgg cgatgcgatg
     1021 gagggcgacc gcacgctgtt cgcccgcgag gactacgtcg aggaggcgtg gcgcatcgtc
     1081 gacccggtgc tgaaagccgg cacgcccgtc cacgaatacg agcccgggac ctgggggccg
     1141 ccggaggtct ccgcggtggc gccaccgggc ggctgggcgg atcccgtcgt gcaggcctga
     1201 gcggtgtcag cggtgcaagt cgaggtcttt gacgatgcgc aggcggtcgc cgaggccgcg
     1261 gccgggtaca tcgccgggca ggcgcgcgtt gcggtggcgg cgcgaggtcg attcctgctc
     1321 gcggtgagcg gcggtcgcac gccatgggtg atgctcgccg cccttgccac gaggccgctg
     1381 ccgtggagcg cgatcgaggt cttccaggtg gacgagcgcg ttgcaccggc gggagatccg
     1441 gaccgcaacc tggtccacct gcgcgaggca ctgctgcagc acgccccgct gcaaccggag
     1501 caggtgcacg cgatgcccgt ggaggccgct gacctcacga gagcggcgcg ggactatgcc
     1561 gccatcctcg aatcgtgcag cggcgtgccg cccgtgctcg acctcgtgca cctcggcctc
     1621 gggccggatg gccacaccgc ctcgttggtt ccgggcgacc cgtcgctcga ggtcagtgac
     1681 gccgacgtga ccctgtcggg tccctaccag ggccatcggc ggatgacgct cacgtatccg
     1741 gtgatcgatc gcgcgcggcg ggtgatgtgg gtcgtgaccg gcgcggagaa ggcggccatg
     1801 ctgcgccggt tgctggatgg cgatcgcgcg attcccgcag gccgcgtatg ccgcgatcgc
     1861 gcgatcgcat tcgtcgaccg cgacgcggcc ggcgacgtgg agcgcgcaca atttcccgga
     1921 agcaacgggg ccgtgccggc ccgaggcaat gcatgaatac caatggctcc tgtgcctggc
     1981 gtggcttggt gtcggtgctc ctgctggcga cggggatggc cggcacggtc cgtgccggcg
     2041 agacggcgac cccgatacaa cgcggccagt accttgcggc ggcgggcgac tgcatttcct
     2101 gtcacaccgt gaacggcggc aagcc
//
LOCUS       DAOMFD010000292         2104 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_31668_length_2104_cov_27.097609, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000292 DAOMFD010000000
VERSION     DAOMFD010000292.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31668_length_2104_cov_27.097609"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(170..757)
                     /locus_tag="PL152_11115"
     CDS             complement(170..757)
                     /locus_tag="PL152_11115"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HQR49878.1"
                     /translation="MTTNTAPSTPGHRINGFESQALTQVVGALTQDRSLARFEFRARN
                     QWIDGGENRSTIQGFYGAGAEDRSRERPFTITNDEPPLLLGNNTAANPGEFVLHALAG
                     CITTTTVLHAAARGINVRSIATRLAGDVDLQGLLDLDPAVRPGYQQIRVTMDIQADCS
                     DEALDELLAFVHSHSPVCDTLMRPVPVTLERVRHD"
     gene            869..1894
                     /locus_tag="PL152_11120"
     CDS             869..1894
                     /locus_tag="PL152_11120"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HQR49879.1"
                     /translation="MTAARHRRPLETLVLALPQTAGSALYGLVDVLTTTGQLWQMLLR
                     RDARTSPFHVQIASTSTRAFRCGNGVPVSPAVSIRTRPAADIVVIPELWLGPDEDLAG
                     RYADVTAWIRARYAGGAHVYSVCSGALLLAETGLLDGREATSHWGYQQLFERRYPRIR
                     FRPEPNLCFSDAAGRLVTAGGATSWQDLALHIVSRHVGPDEALRMAKAFLLKPHEEGQ
                     LPYRPLVKPRRHSDSAVRRCELWLAEHFRERHAVARVVAYSGLAERTVKRRFKAATGA
                     TLIDYLQDVRIEEAKRLLETTQRAAEAISVEVGYQDASFFRRLFSRRTGLTPSGYRRM
                     FQPLRRS"
BASE COUNT          296 a          704 c          742 g          362 t
ORIGIN      
        1 gttgtcctcg acgagcagca gcgttgagcc agggctctgc ggcatgagtg gatcgtggtc
       61 gtcgtctctg acgttgttcg ccggttgata tcacaggtcc cgggccccgt cgactgacgg
      121 gtccggcgat gccgggccac cggccttgcg gccggcggcc cgcgcgccgt cagtcgtggc
      181 gcacccgctc gagcgtcacc ggcaccggcc gcatcagcgt atcgcaaacc ggcgagtgcg
      241 aatggacgaa cgccaggagt tcgtcgagcg cctcgtcgct gcagtccgcc tggatgtcca
      301 tcgtcaccct gatctgctgg taccccggcc gcaccgcggg gtcgaggtcg agcaggccct
      361 gcagatcgac gtcgcccgcg agccgcgtgg cgatcgagcg cacgttgatc ccgcgagccg
      421 cggcatgcag gacggtcgtc gtcgtgatgc aaccggcgag ggcgtgcagc acgaactcgc
      481 ccgggttcgc ggccgtgttg ttgccgagca gcaggggcgg ctcgtcgttc gtgatggtga
      541 atggccgttc gcgcgaccga tcctcggcgc cggctccgta gaagccctgg atggtggagc
      601 ggttttcgcc gccgtcgatc cactggttgc gggcccggaa ctcgaacctt gcgaggctgc
      661 ggtcctgcgt cagtgcgccg acgacctgcg tgagcgcctg gctctcgaag ccgttgatgc
      721 ggtgacccgg ggtcgacggt gcggtgttcg tggtcatgag gattctcctt ggcgacgggg
      781 tggaaacgtg gctcaacgat aggcatccga gggcacggcc gtaagcggcg cgcttgccgc
      841 tttcggtcgt ttcatgccag tgtgtgtcat gactgctgcc cgccaccgtc ggccgctcga
      901 gacactggtg ctcgccctgc cgcagacggc aggttccgcg ctgtacgggc tcgtcgacgt
      961 gctgacgacg accggccagc tgtggcagat gctgctccgt cgagacgcac ggacttcgcc
     1021 gttccacgtg cagatcgcat cgaccagcac ccgcgcgttt cgctgcggca acggcgtccc
     1081 ggtttccccg gccgtgtcga tccggacccg tccggcagcg gacatcgtcg tcatccccga
     1141 actgtggctc ggaccggacg aggacctcgc gggccgctac gccgacgtca cggcctggat
     1201 ccgcgcgcgg tacgcgggcg gcgcacacgt atattccgtg tgttccggag cgctgctgct
     1261 cgcggagacg gggctgctgg acggaaggga ggcgacgtcg cactggggtt accagcagct
     1321 gttcgagcgg cgctatccgc ggatccgctt ccggccggag ccgaacctgt gtttctccga
     1381 tgcagcgggt cgcctggtca ccgctggcgg agccacgtcc tggcaggacc tcgcgctgca
     1441 catcgtgtcc cggcacgtcg gcccggacga ggcgctgcgg atggccaagg cctttctcct
     1501 caagccgcac gaagagggcc agctgcccta tcgcccgctg gtgaagccgc ggcggcactc
     1561 ggactcggcg gtacgccgtt gcgaattgtg gctggccgag catttccgcg agcgccatgc
     1621 cgtcgcaagg gtcgtggcgt actcgggact cgccgagcgc accgtcaagc gccgcttcaa
     1681 ggcggccact ggcgcgacgc tgatcgacta tctgcaggac gtgcgtatcg aagaagcgaa
     1741 gcgcctgctc gagacgacgc agcgcgccgc ggaggcgatc agcgtcgagg tcggctatca
     1801 ggacgcgtcg ttcttccgac gcttgttcag tcggcgcacc ggcctgacgc cgtcaggcta
     1861 ccgacgcatg ttccagccgc tacgccgatc atgatcccgc gtgcgcccgc caggccacga
     1921 ctttcggacc ggcaagcagg tgaagctgca gctgagcgtc ccttcggatc agtcggtgcg
     1981 accacgctga tcgtcagggt gcggcactgc gtagcgagcc gtcgtcagcg aatgccggcg
     2041 ccggccaggg cgtcgggctg ccagtcggac ttcgggaacg gtccgcgctg ctcgatgccg
     2101 tagt
//
LOCUS       DAOMFD010000293         2098 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_31834_length_2098_cov_32.129222, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000293 DAOMFD010000000
VERSION     DAOMFD010000293.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2098)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2098
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31834_length_2098_cov_32.129222"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..473
                     /locus_tag="PL152_11125"
     CDS             <1..473
                     /locus_tag="PL152_11125"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HQR49880.1"
                     /translation="QSTRPGWMLDLSYGFRGGEMDGKPRPDMVTAMVSMDLPLFRGNR
                     QDREVAAARAEAEGLHEMHKDHQREMSAMLQEAWGVVDRTGQLERYYETDLVPLADQT
                     VQAALLAYRNNRAMVDDVVSARRVALETNLKRLRLAADRAQAQYELDYLVGAEP"
     gene            470..1717
                     /locus_tag="PL152_11130"
     CDS             470..1717
                     /locus_tag="PL152_11130"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HQR49881.1"
                     /translation="MNSRVTGLIAGFVVAGLLAAGCSRQETPAQPSAGAAGDAASAAP
                     AKPLYYRHPHDPQRTSAVPMKDEMGMDYVPVFADAAGPEVRISPTVMNNLGVRVEPAA
                     MGALPRTADTVGYVGFDERNVRQVRPRAEGWVESLAVRTMGSTVRAGQPLFTLYSPML
                     ESAQQEYVDAMRIGNADLVAASKDRLGALGLDKATAERLGKSGKVSGHVTYTAPISGV
                     VTELELREGSMVTPEMVAMTITGLGSLWVIAEVPEAQSGWVATGTAAELSFPSLPGRR
                     VSGHVEYVYPELDRETRTVKARITLDDAPPEIRPNMLASITLVGRAGPEVVSIPRSAL
                     IRTGGGDRVVIALGDGRFAPRRVVAGHESGERIEIRDGLVAGENVVVAGQFLLDSEAN
                     LRAGLGRLGEESPSPAPADTARP"
     gene            1737..>2098
                     /locus_tag="PL152_11135"
     CDS             1737..>2098
                     /locus_tag="PL152_11135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011711636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49882.1"
                     /translation="MINSIIRASLENRALVALLAALLTAVGIYSFAHTPVDALPDLSD
                     VQVIVRTPYAGQAPRVVEDQVTYPLTTALLSVPGATTVRGYSFYGDSFVNVLFADGTD
                     PYWARSRVLEYLSQAEARL"
BASE COUNT          337 a          706 c          753 g          302 t
ORIGIN      
        1 agcagtccac gcggccgggg tggatgctcg acctgagcta cggattccgc ggcggcgaga
       61 tggacggcaa gccgcgtccc gacatggtca ccgcgatggt gagcatggac ctgccgctgt
      121 tccgcggtaa ccggcaggac cgcgaagtcg cggcagcgcg ggccgaggcg gaaggcctgc
      181 atgaaatgca caaggaccat cagcgcgaga tgagcgccat gctgcaggaa gcatggggcg
      241 tggtggaccg caccggccag ctcgagcggt actacgagac cgacctcgtg ccgctcgccg
      301 accagaccgt gcaggccgcg ctgctcgcat accgcaacaa tcgcgcgatg gtcgacgacg
      361 tggtgagcgc tcgacgcgtc gcgctcgaga cgaatctgaa acgcctgcga ctcgccgctg
      421 accgtgccca ggcgcaatac gaactcgact acctcgtcgg agcagagcca tgaacagccg
      481 tgtcacgggc ctgatcgcgg gattcgtcgt cgccgggttg ctggcggccg gatgcagtcg
      541 gcaggagacg ccggcgcagc cgtctgccgg agccgcgggc gacgcggcca gtgctgcgcc
      601 ggcgaagccg ctctattacc gccatccgca tgatccgcag cgcacgtccg cagtgccgat
      661 gaaggacgag atggggatgg actacgtgcc ggtatttgcc gacgcagccg gccccgaagt
      721 ccgcatctcg ccgacggtca tgaacaacct cggcgtgcgc gtcgagcccg cggcgatggg
      781 tgcgctgccg cgcacggccg acacggtcgg gtacgtgggc ttcgacgaac gcaacgtgcg
      841 acaggtgcgt ccgcgcgcag aagggtgggt ggagagcctc gccgtgcgga cgatgggatc
      901 gaccgtccgg gccgggcagc cgctcttcac gctgtactcg ccgatgctcg agtcagccca
      961 gcaggaatat gtcgatgcaa tgcgcatcgg caacgcggac ctcgtcgcgg cgtcgaagga
     1021 ccggctcggc gcgctcggcc tcgacaaggc aacggccgag cggctcggga aatccggcaa
     1081 ggtgtccggc cacgtcacct acacggcgcc gatctcgggc gtcgtcacgg aactcgagtt
     1141 gcgcgagggc tcgatggtga ccccggagat ggtggcgatg accatcaccg ggctcggcag
     1201 cctgtgggtc atcgccgagg tcccggaggc gcagtcgggc tgggttgcga cgggcaccgc
     1261 ggcggagctg tcgtttccct cgctgccggg tcgtcgcgtg tcgggccacg tcgagtacgt
     1321 atacccggaa ctcgacaggg agacacgcac cgtcaaggcg cgcatcaccc tcgatgacgc
     1381 gccccccgaa atacgcccga acatgctcgc gtccatcacg ctggtcggca gggcgggccc
     1441 cgaggtcgtc agcatcccgc gcagcgcgtt gatccgcacc ggcggcggcg accgcgtcgt
     1501 gatcgcgctc ggggacggcc gcttcgcgcc gcgccgcgtc gtggcgggcc acgagagcgg
     1561 cgagcggatc gagattcgcg acggcctggt ggctggcgag aacgtcgtcg tcgcaggcca
     1621 gttcctgctg gactccgaag ccaacctgcg ggccggcctc gggcgcctcg gcgaggaatc
     1681 gccatcacca gccccggccg acactgccag gccatgagcg ggcggaggat gcgctggtga
     1741 tcaattcaat catccgggcc tcgctcgaga accgggcgct ggtggccctg ctcgccgcgc
     1801 tgctcacggc agtcggcatc tactcgtttg cgcacacgcc cgtggacgcg ttgccggact
     1861 tgtcggacgt ccaggtcatc gtgcgcacgc cgtacgctgg gcaggcaccg cgagtcgtcg
     1921 aggaccaggt gacgtatccg ctcaccacgg cgctgctgtc cgtgccgggc gcgacgacgg
     1981 tgcgcggcta ctccttctac ggcgactcgt tcgtgaacgt gctgttcgcg gacggcaccg
     2041 acccgtactg ggcgaggtcc cgcgtgctcg agtacctgag ccaggcggag gcgcggct
//
LOCUS       DAOMFD010000294         2090 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_32057_length_2090_cov_35.154791, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000294 DAOMFD010000000
VERSION     DAOMFD010000294.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2090)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2090
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32057_length_2090_cov_35.154791"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..370)
                     /locus_tag="PL152_11140"
     CDS             complement(<1..370)
                     /locus_tag="PL152_11140"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HQR49883.1"
                     /translation="MRFIYAAVALVGLVASPAYAAGDGFYLGGGVGYFQLDLGHGWDA
                     GDTAFKVEGGYNFAKYFAADVEWIYGGRLKSEGLAWDLDGWNFSLVGRYPIDQQFDVF
                     AKLGAFMWNADGKSCNYAFCA"
     gene            complement(540..>2090)
                     /gene="polA"
                     /locus_tag="PL152_11145"
     CDS             complement(540..>2090)
                     /gene="polA"
                     /locus_tag="PL152_11145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HQR49884.1"
                     /translation="SRAKLGHHLKYDAHVLRNHGIELQGMEFDSMLESYVLDSTATRH
                     DLDSVAQHYLGVSTIHYEDVAGKGAKQLPFAEVPVDRAGEYSAEDADVAMRLHAALWP
                     RIEALPKVRRLYEEIEQPLVTVLLDMEHTGVLIDAAMLKRQSHELSKQLLELQAKAHA
                     VAGGPFNLDSPKQLQEVLFGKLGLPVQRRTSTGQPSTAEDVLEELADEYELPRIVLDY
                     RGMAKLRSTYTDKLPEQVNPRTGRVHTSYHQAIAATGRLSSTDPNLQNIPIRTPEGRR
                     IRQAFVAPPGHVLVSADYSQIELRIMAHLSGDEGLLRAFAEDRDIHQATAAEVFGLEP
                     AKVAPEQRRAAKAINFGLIYGMSAFGLARQLGIERAAAQQYVDLYFQRYPGVKRYMDE
                     TRARAREQGYVETVAGRRLYLPDIRSRNQQLRQYAERSAINAPMQGTAADIIKRAMID
                     VHRWLADGRTGARLVMQVHDELVLEVAASAVDQVVTGLRERMSAAGDLRVPLKVEVGR
                     GSNWDEAH"
BASE COUNT          310 a          728 c          693 g          359 t
ORIGIN      
        1 ccgcgcagaa ggcgtagttg cagctcttgc cgtcggcatt ccacatgaag gcgccgagct
       61 tcgcgaacac gtcgaactgc tggtcgatcg ggtagcgccc gacgagggag aaattccagc
      121 cgtcgaggtc ccatgccagg ccttcactct tcaggcggcc gccgtagatc cactcgacgt
      181 cggcggcgaa gtacttggcg aagttgtagc cgccttcgac tttgaaggcc gtgtcgccgg
      241 cgtcccagcc gtggccgaga tccagctgga aatatcccac gccgccaccc aggtagaacc
      301 cgtcacccgc ggcatacgca ggggacgcga ccaggccgac aagcgcaacc gccgcgtaga
      361 tgaatcgcat ggtgcctccc taccggttcg gttccggcat cgtatcgggg cgcgagatcc
      421 tcaccacgac gtgcctgaaa tccctccgga ccgcgcccat gctaggtgtc gtcagcctcg
      481 cgacgcaagg cgggattgca tacgggcgca gcagcctgtc gcgtggcacg cgaaccgctt
      541 cagtgcgctt cgtcccagtt cgagccgcgc ccgacctcga ccttgagcgg gacccgcagg
      601 tcacctgcgg ccgacatgcg ctcgcgcagt cccgtcacga cttgatcgac ggcgctcgcc
      661 gccacctcga gcacgagctc gtcgtgcacc tgcatgacca gccgcgcgcc ggtccgaccg
      721 tcggcgagcc agcggtgcac gtcgatcatc gcccgcttga tgatgtccgc cgccgtgccc
      781 tgcatgggcg cgttgatggc gctgcgctcc gcgtactggc gcagctgctg gttcctcgag
      841 cggatgtcgg gcaggtacag gcgacggccc gcgaccgtct cgacgtagcc ttgctcgcgc
      901 gccctggcac gcgtctcgtc catgtagcgc ttcacgcccg ggtagcgctg gaagtacagg
      961 tccacgtact gctgcgccgc ggctcgctcg atgcccagtt gccgcgccag cccgaacgcc
     1021 gacatgccgt agatgaggcc gaaattgatc gccttcgcgg cacgtcgctg ctcgggcgcg
     1081 accttcgcgg gctcgagccc gaagacctcg gcggccgtcg cctggtggat gtcgcggtcc
     1141 tcggcgaatg ccctgagcag accctcgtcc ccggacaggt gcgccatgat gcgcagctcg
     1201 atctgcgaat agtccgcgga caccagcacg tggcccggcg gtgcgacgaa agcctgccgg
     1261 atgcgacgtc cctcgggcgt gcggatcggg atgttctgca ggttcgggtc ggtcgacgag
     1321 aggcgccccg tcgctgcgat ggcctggtgg tacgaggtgt gcacgcgccc ggtccgcgga
     1381 ttcacctgct cgggcagctt gtccgtgtac gtcgagcgca gcttcgccat gccgcggtag
     1441 tcgagcacga tgcgcggcag ctcgtactcg tcggcgagtt cctcgagcac gtcctcggcg
     1501 gtcgacggct gcccggtcga cgtccggcgt tgcacgggca gcccgagctt gccgaacagc
     1561 acctcctgca gctgtttcgg cgagtcgaga ttgaagggcc cgccggccac ggcgtgcgcc
     1621 ttcgcctgca gttccagcag ctgcttcgac agctcgtggc tctgtcgctt gagcatcgcc
     1681 gcatcgatca gcacgccggt gtgttccatg tcgagcagca cggtcacgag cggctgctcg
     1741 atctcctcgt acagccggcg caccttcggc aacgcctcga tgcggggcca cagggcagcg
     1801 tgcagccgca tcgcgacgtc ggcatcctcc gccgagtatt cccctgcgcg gtcgaccggc
     1861 acctcggcga acggcagctg tttcgcgccc ttgcccgcga cgtcctcgta gtggatcgtc
     1921 gacacgccga ggtagtgctg cgccaccgaa tcgaggtcgt ggcgcgtcgc cgtgctgtcg
     1981 agcacgtagg actcgagcat cgagtcgaac tccatgccct ggagctcgat gccgtggttg
     2041 cgcagcacgt gggcgtcgta cttgaggtgg tggccgagct tggcgcgcga
//
LOCUS       DAOMFD010000295         2088 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_32109_length_2088_cov_35.249877, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000295 DAOMFD010000000
VERSION     DAOMFD010000295.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32109_length_2088_cov_35.249877"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PL152_11150"
     CDS             <1..914
                     /locus_tag="PL152_11150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HQR49885.1"
                     /translation="RDLEQVMLDFYHKRANLLLCTTIIESGIDVPTANTIIIDRADRF
                     GLAQLHQLRGRVGRSHHQAYAYLLAPPRNLMTTDAAKRLEAIESLEDLGAGFVLATHD
                     LEIRGAGELLGEGQSGQIQEIGFALYMELLERAVAAMRSGRVPELEKPLHHGPELDLH
                     VPALLPEPYLPDIHARLVLYKRVAGVASELELDELQSETVDRFGVLPEPARNLFRIAR
                     LRVAAARLGIERLDVGPAGGSIAFGPDTPVDPGALILLVQKSNRALRFDGPTRIRFGG
                     DYPAAEDRYAVAQSRLDALARCVTRPS"
     gene            complement(898..1146)
                     /locus_tag="PL152_11155"
     CDS             complement(898..1146)
                     /locus_tag="PL152_11155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49886.1"
                     /translation="MYSNLKALLLAIYLLAIVSACCVALPGGIWLQKVAVILVALHVI
                     EVAIAFDSVKRHPGPLVDSIALTLLFGFLHWLPLRKAV"
     gene            1280..1984
                     /locus_tag="PL152_11160"
     CDS             1280..1984
                     /locus_tag="PL152_11160"
                     /inference="COORDINATES: protein motif:HMM:NF013488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family oxidoreductase"
                     /protein_id="HQR49887.1"
                     /translation="MRVDVWSDVVCPFCYIGKRRLEAALAGLPHSDTVEVVWHAFQLL
                     PDLVTDPTRNAVQNMAERKGWPMAYARQVAGQLTALAAADGLAFHYEQTRVANTFDAH
                     RLQHFATTQGKGDAMAEALFKAFFTDGRNIADPDTLAALASAVGLQESQAREVLASDA
                     HAEDVRRDIDAALQLGVTGVPFFVIDGRYAVSGAQDSSIFRQALQRALADPTARERAP
                     ANDMDASACRVDGECP"
BASE COUNT          341 a          722 c          708 g          317 t
ORIGIN      
        1 agcgggacct cgagcaggtg atgctcgatt tctaccacaa gcgcgccaac ctgctgctgt
       61 gcactacgat catcgagagc ggcatcgacg tgcccaccgc gaacacgatc atcatcgacc
      121 gggcggatcg cttcggcctc gcgcagctcc accagctcag gggccgcgtc ggacggtctc
      181 atcaccaggc ctatgcctat ctcctggcgc cgcccaggaa cctgatgacg acggacgccg
      241 ccaagcgcct ggaagccatc gagtcgctgg aggacctcgg cgccgggttc gtgctggcga
      301 cccacgacct ggagatccgc ggcgcgggcg agctgctcgg cgaaggccag agcggccaga
      361 tccaggagat cggctttgcg ctgtacatgg aactgctcga acgcgccgtt gccgcgatgc
      421 gctccggccg cgtgcccgag ctggagaagc cgctgcatca tgggccggag ctggacctgc
      481 acgtgccggc cctgttgccg gagccatacc tgccggacat ccacgcgcgg ctcgtgttgt
      541 acaagcgggt ggccggcgtc gcttccgagc tggaactcga cgaactgcag tccgaaacgg
      601 tggatcgctt cggcgtgttg cccgagccgg cgcgcaacct gttccggatc gcgcggctca
      661 gggtcgcggc ggcgcggctc ggcatcgagc ggctggacgt ggggcccgcc ggcgggtcga
      721 tcgctttcgg gccggacaca cccgtggatc cgggcgcgct gatcctgctc gtccagaaaa
      781 gcaatcgcgc cctgcgcttc gacgggccga cccgcatccg cttcggcggc gactaccccg
      841 cggcggaaga ccgctatgcg gtcgcgcagt ccaggctcga cgcgctcgcg cgctgcgtca
      901 cacggccttc ctgagcggca gccagtgcag gaagccgaac agcagcgtca acgcgatgct
      961 gtcgacgagc ggccccggat gacgcttgac gctgtcgaat gcgatggcca cctcgatcac
     1021 gtgcagcgcg accaggatga ccgccacctt ctgcagccag atcccgcccg gcagcgcgac
     1081 gcagcaggcg gagacgatgg cgagcaggta gatcgcgagc agcagcgcct tgaggttcga
     1141 atacatgtcg ggctccctgg ccgttccggg cattgcgtgg gaccatgcta catgcaatcg
     1201 gacgtggcgg gccccgacat tttcgcggcc gtgagctcca cggagtgcgc accgcatgcc
     1261 gagcgcaggt gaaccggcca tgagagtcga tgtctggagc gacgtggtct gtcccttctg
     1321 ctacatcggc aagcgccggc tcgaagctgc gctcgccggc ctgccgcaca gcgacacggt
     1381 ggaggtcgtc tggcacgcgt tccaactgct gcccgacctc gtcaccgacc ccacccggaa
     1441 cgcggtgcag aacatggccg agcgcaaggg ctggccgatg gcgtacgcgc ggcaggtcgc
     1501 cgggcaactc accgcactgg ccgctgccga cggcctggcg ttccactacg agcagacccg
     1561 ggtcgccaac acgttcgatg cccaccggct gcagcacttt gcgacgacgc agggcaaggg
     1621 cgacgcgatg gccgaagccc tgttcaaggc attcttcacc gatggccgca acattgccga
     1681 ccccgacacg cttgcggcat tggcctcagc cgtggggttg caggagtcgc aggcgcgcga
     1741 ggtgctcgcg tcggatgcac acgccgagga cgttcgccgc gacatcgacg cagccctgca
     1801 gctgggcgtc accggtgtcc cgttcttcgt catcgacgga cggtatgccg tgagcggggc
     1861 ccaggacagc tcgatctttc gccaggcgtt gcagcgagcc ctcgcggacc cgaccgcccg
     1921 ggagcgtgcg ccggcgaacg acatggacgc gtccgcatgc cgagtcgacg gcgaatgccc
     1981 gtgatgcggc ggaaacgcgg ctggcgatag tcccggtgct ggctacaggc ttacgccgac
     2041 gactacgttt ccgcccgacc gcttcgcgct ctcgactgcg cgttgtga
//
LOCUS       DAOMFD010000296         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_32235_length_2084_cov_34.164120, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000296 DAOMFD010000000
VERSION     DAOMFD010000296.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32235_length_2084_cov_34.164120"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PL152_11165"
     CDS             complement(<1..518)
                     /locus_tag="PL152_11165"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HQR49888.1"
                     /translation="MAPADARDDRRLRALIEERLRGTAPGGDPLAHLTRDRTPEEVEV
                     VRRLVPTQYSRAAVLVPIVERAEGLTLLLTRRASHLKNHPGQISFPGGRVESADEGPI
                     AAALRETEEEIGLARTHVSPIGYLADHAVISGYVVTPVVAFVRPGFALALDSTEVEEV
                     FEVPLAFVLDPV"
     gene            complement(519..1925)
                     /locus_tag="PL152_11170"
     CDS             complement(519..1925)
                     /locus_tag="PL152_11170"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HQR49889.1"
                     /translation="MAADDFRLERDSMGELRVPAGALWGAQTQRAVQNFQLSGLRMPR
                     GFIRALGLVKWAAAGANLELGELDKVRAFAIQRAALEVAEGRHDAHFPVDVFQTGSGT
                     SSNMNANEVIAHLATAYAGGQPVHPNDDVNRSQSSNDVVPTAIHLSAALAVAEQLLPA
                     LQELSETIGRKAADVGEIVKTGRTHLMDAMPLTLGQELGAWRTQVEDSIARIRACRPR
                     LHCLAQGGTAVGTGVNAPAGFAAAVAALLSRQTGLDLRPSRDFFASLSSQDTAVELSG
                     QLRTLATCLMKIANDLRWMNSGPLAGLGEVALPALQPGSSIMPGKVNPVAAEAVAMVA
                     AKVIGNDLTVTIAGQSGNFQLNVMLPVIAHDLLQSIDLLANASRSLARSAIAGFAVNT
                     RQVAAALERNPMLVTALNPVIGYDKAAEIAKKAYLEGRPIRQVAAEMTDLGADRLAKL
                     LDPAALTRGGVRRGKAGD"
BASE COUNT          289 a          754 c          715 g          326 t
ORIGIN      
        1 tggactggat cgagcacgaa cgcgagcggc acttcgaaca cctcctccac ctccgtcgag
       61 tccagcgcga gcgcgaagcc gggccggacg aatgcgacga ccggcgtgac gacgtatccg
      121 gagatcacgg cgtggtcggc gagatagccg atcggcgaca cgtgagtccg ggcaaggccg
      181 atctcttcct ccgtctcgcg cagggcggcg gcgatcggtc cctcgtccgc ggattccacg
      241 cggccgccgg ggaagctgat ctgccccggg tgattcttga ggtgggacgc gcgccgtgtg
      301 agcagtagag tcagtccctc cgcacgctcg acgatcggca cgagcaccgc cgcgcgcgag
      361 tactgggtcg ggacgagacg gcggacgact tcgacctcct ccggagtccg gtcgcgtgtc
      421 aggtgcgcga gcgggtcccc ccccggcgcg gtgccgcgca gccgctcctc gatcagggcc
      481 ctgagccgcc ggtcgtccct ggcatccgcg ggcgccactc agtcccccgc cttgccgcgc
      541 cgcacacctc ccctcgtcag ggccgcaggg tcgagcagct tcgccaggcg gtccgcgccg
      601 aggtccgtca tctccgcggc cacctgccgg atcgggcggc cctcgaggta cgccttcttg
      661 gcgatttccg ccgccttgtc ataaccgatg accggattga gggccgtcac cagcatcggg
      721 ttgcgctcga gcgccgcggc gacctgccgg gtgttgaccg caaatcccgc gatggcgctg
      781 cgagccaggc tgcgcgatgc attggccagc aggtcgatgc tctgcaggag gtcgtgcgcg
      841 atcacgggca gcatcacgtt caactggaag ttgccggatt gtccggcaat cgtgacggtc
      901 aggtcgttgc cgatcacctt cgccgccacc atcgccaccg cctcggccgc gaccgggttg
      961 accttgccgg gcatgatgct gctgccgggc tgcagcgccg ggagcgccac ttcgccgaga
     1021 ccggcgagcg ggccgctgtt catccagcgc aggtcgttcg cgatcttcat caggcaggtc
     1081 gccagcgtgc gcagctggcc ggacagttcg acggcggtgt cctgcgagct caacgaggcg
     1141 aagaaatccc ggctcggacg caggtccagc ccggtctgcc gcgacaggag cgccgcgacg
     1201 gcagcggcaa atccggcggg cgcattgacc ccggtcccca cggcggtccc gccctgcgcc
     1261 aggcaatgca gcctcggccg gcatgcgcgg atgcgcgcga tcgagtcctc gacctgcgtg
     1321 cgccacgcgc cgagctcctg gccgagcgtg agcggcatgg cgtccatcag gtgggtccgc
     1381 ccggtcttca cgatctcgcc gacgtccgcc gccttgcggc cgatcgtctc ggacagctcc
     1441 tgcagggccg gcaacagctg ctcggccacc gcgagagcag cgctcaggtg gatcgcggtc
     1501 ggcacgacgt cgttgctgct ctggctgcgg ttgacgtcgt cgttggggtg gaccggctgg
     1561 ccgcccgcat aggcggtggc gaggtgcgcg atcacctcgt tcgcattcat gttgctgctg
     1621 gtcccggaac cggtctggaa cacgtccacc gggaagtgcg cgtcgtggcg gccctccgcc
     1681 acctcgagcg ccgccctctg gatggcgaac gcccgcacct tgtcgagttc gcccagctcg
     1741 agattggccc cggccgcagc ccacttcacg agcccgaggg cgcggatgaa cccgcgcggc
     1801 atgcggaggc cgctcagctg gaaattctgg accgcgcgct gggtctgcgc gccccacagc
     1861 gcgcccgccg gtacgcgcag ttcgcccatg ctgtcgcgtt cgaggcgaaa gtcgtctgcc
     1921 gccatgccac tccggctccg ggaagtccgc gcaagcgccg cttgctgcgg ctgcgctatc
     1981 atttcggatc gtagcacctc ccggattgcc gcatgagcct cgacaccctg acggccctct
     2041 cgccggtcga cggacggtac gccagccgct gcacggagct gcgc
//
LOCUS       DAOMFD010000297         2084 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_32236_length_2084_cov_30.473632, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000297 DAOMFD010000000
VERSION     DAOMFD010000297.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2084)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2084
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32236_length_2084_cov_30.473632"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1351
                     /gene="purB"
                     /locus_tag="PL152_11175"
     CDS             <1..1351
                     /gene="purB"
                     /locus_tag="PL152_11175"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020648851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HQR49890.1"
                     /translation="TALSPVDGRYASRCTELRELFSESGLIRARVRVEVAWLLALAAS
                     PDIPEMHGLSQSDLEVATRLEAAFTEADAAAVKAIERETNHDVKAVEYFVKDRLREHA
                     AWRPRLEFVHFACTSEDINNVAYALLCLQARDEILLPRLEALAETLRAMAHTHAHDAM
                     MSRTHGQPATPTTLGKEVAVFVHRLRRACERLRAVPILGKMNGAVGNYNAHLAAYPDV
                     DWPGTARRLVESLGLQWNPYTTQIDPHDWMAEYFDAVARANTVLIDLCRDFWGYISVG
                     AFRQKVIAGEVGSSTMPHKVNPIDFENAEGNFGVANALLRFMADKLPVSRWQRDLTDS
                     TVQRNIGVALAHSDVAIQSVSRGLARLEVDRTQLAAELDANWELLAEPIQTVMRRYAL
                     PGAYEQLKALTRGRRLDAAALADFIDGLPLPAEAKGRLRELTPASYLGLAARLARDV"
     gene            1344..1850
                     /locus_tag="PL152_11180"
     CDS             1344..1850
                     /locus_tag="PL152_11180"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HQR49891.1"
                     /translation="MSERRPAAPVRIRPATWQADRDDLMRVRRRVFIEEQGIPESEEM
                     DEHDPVCRHALALAPSGNVIGTGRLTPAGKIGRISVLPAYRRRGVGGALVVHLVNQAT
                     ELGLAQVYLHAQADSVGFYERLGFKPEGPVFDEVGIPHRRMRRGIERSDGNQAGRDGN
                     EEHPLDAR"
     gene            1864..>2084
                     /locus_tag="PL152_11185"
     CDS             1864..>2084
                     /locus_tag="PL152_11185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49892.1"
                     /translation="MAELATRAQRSLSIYTPDLEPQLYDQDCFLEPMKRLVLARSHAR
                     LRVLISDPARASREGNRFMQMARRLTSYI"
BASE COUNT          334 a          748 c          696 g          306 t
ORIGIN      
        1 gacggccctc tcgccggtcg acggacggta cgccagccgc tgcacggagc tgcgcgagct
       61 cttcagcgag tccggcctga tccgggcccg ggtccgcgtc gaggtcgcct ggctgctggc
      121 cctggcggcg agccccgaca tccccgagat gcatggcctg tcgcaatcgg atctcgaggt
      181 cgcgacgcgg ctcgaggcgg ccttcacgga ggccgatgcc gctgcggtca aggccatcga
      241 gcgcgagacc aaccacgacg tcaaggccgt cgaatacttc gtcaaggaca ggctgcgcga
      301 gcacgcggcc tggcgcccga ggctcgagtt cgtgcatttc gcgtgcacgt ccgaggacat
      361 caacaacgtg gcctatgcgc tgctctgcct gcaggcgcgc gacgagatcc tgctgccacg
      421 cctcgaagcc ctggccgaga cgctgcgcgc gatggcacac acgcatgcgc acgacgcgat
      481 gatgtcgcgc acgcacgggc agccggcgac gccgaccacg ctcggcaagg aggtcgccgt
      541 cttcgtgcat cgcctgcggc gcgcctgcga gcgactgcgc gccgtaccga tcctgggcaa
      601 gatgaacggg gccgtcggca actacaacgc gcacctcgcg gcgtaccccg acgtcgattg
      661 gcccggcacg gcgcggcggc tggtggagtc gctcgggctg cagtggaacc cctacacgac
      721 gcagatcgat ccgcacgact ggatggccga gtacttcgac gccgtggcgc gtgccaacac
      781 ggtgctgatc gacctgtgcc gcgacttctg gggctacatc tccgtcggtg ccttccggca
      841 gaaggtcatc gccggcgagg tcggttcctc gaccatgccg cacaaggtca acccgatcga
      901 cttcgagaac gccgagggca acttcggcgt ggccaacgca ttgctgaggt tcatggcaga
      961 caagctcccg gtgtcacggt ggcaacggga cctgaccgac tccaccgtcc agcgcaacat
     1021 cggcgtcgcg ctcgcccact cggacgtggc gatccagtcg gtgtcacgcg gcctggcccg
     1081 gctcgaggtg gaccggaccc agctcgccgc cgagctcgac gccaactggg aattgctcgc
     1141 cgaaccgatc cagaccgtga tgcggcgtta cgcgctgccc ggagcctacg agcagctcaa
     1201 ggcgctgacc cgcggacgcc gcctcgatgc cgctgcgctc gcggacttca tcgacggcct
     1261 gccgctgccg gccgaggcca agggccggct gcgcgagctg acacccgcga gctaccttgg
     1321 cctcgcggcc aggctggccc gcgatgtctg agcgacggcc cgctgcaccg gtgcgcatcc
     1381 gcccggccac ctggcaggcg gatcgcgacg acctgatgcg cgtgcggcgc cgcgtgttca
     1441 tcgaggaaca gggcatcccc gagtccgagg aaatggacga gcacgacccg gtctgccggc
     1501 atgcgctcgc cctggccccc agcggcaacg tcatcgggac gggtcgcctc acccccgctg
     1561 gcaagatcgg ccgcatttcg gtgctgcccg cataccgccg gcggggcgtg ggcggagcgc
     1621 tggttgtcca tctcgtgaac caggccacag aactcgggct ggcgcaggta taccttcacg
     1681 cgcaggccga ttccgtgggc ttctacgagc ggctgggctt caagcccgag ggaccggtgt
     1741 tcgacgaagt gggcatcccg caccgacgca tgcgccgcgg gatcgagcgg agcgatggaa
     1801 accaggctgg acgtgacggg aacgaggagc atcctctcga cgctcgatga gtatcgggcc
     1861 gccgtggccg aactcgccac ccgcgcccag cgttcccttt cgatctacac gcccgaccta
     1921 gagccccagc tgtacgacca ggactgcttc ctcgagccga tgaagcgcct tgtgcttgcg
     1981 cgcagccacg cgcggctccg cgtgctcatc tccgaccccg ctcgcgcctc ccgcgagggc
     2041 aatcgcttca tgcagatggc ccgtcgcctg acgagctaca tcga
//
LOCUS       DAOMFD010000298         2081 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_32332_length_2081_cov_31.178677, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000298 DAOMFD010000000
VERSION     DAOMFD010000298.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32332_length_2081_cov_31.178677"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="PL152_11190"
     CDS             complement(<1..616)
                     /locus_tag="PL152_11190"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HQR49893.1"
                     /translation="MIQDLVISIVFITYAISVLMGFGTILTWVERKQAAVMSDRIGAN
                     RAYFRIPFTQVKLVWWGLFHGMADGLKMILKEDFKPKTYDWYAYAVAPWVVFTPVLLV
                     FAVIPFGGVLVPGHLIPMLADWFGDKSYPMQIARLDAGLLMVFAFGGMTIIGAMLAGW
                     SSSNKFSLLGALRAGSQMISYEVIMGLTVLGLIVIFGTVDLNSIV"
     gene            complement(613..>2081)
                     /locus_tag="PL152_11195"
     CDS             complement(613..>2081)
                     /locus_tag="PL152_11195"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HQR49894.1"
                     /translation="LPSRRPRMPDFFINGQPVTAAKGQTVMQAAKAAGFFIPTFCWHP
                     NLSISGNCRICVVEVEGEGGGSSWMEIACNMPVTEGMRVLTDSEAVRARRRETLQFIL
                     LNHPVDCGICDKAGECTLQDYHYEMNGLPSVSTVPKVHATKYFELSSRIVLDNERCIV
                     CSRCVRFTREISKSHALGIQNRGDASLVRPVESRDFESDPYSDNVIDICPVGALLSKQ
                     FLHKARVWYLKATPSVCPGCSRGCTVDLWHRKAEWKLNALDPRENTAIARVTPRENPE
                     VNGPWICNKGRDLARIFERPRAEQAMQKGKPAALGDAIAAARRLIGEAKRPAALVSSW
                     GSNEELAAFKATLGDRVECIVKSDWQAQPGERLEDDLLIRADKNPNTRRAQELFGAEA
                     HLVPAESDLVLVWGEGASFADLPRGAKVILLGSYLAPENGHADVFIPVSIQTERAGHY
                     TNFAGVVSRFEPIVRKKDGVVHAEQLFAQLAARSEAIA"
BASE COUNT          366 a          691 c          660 g          364 t
ORIGIN      
        1 gcacgatcga gttgagatcc accgtgccga agatgacgat caggcccagc accgtcaggc
       61 ccatgatgac ttcgtaggag atcatctggc tgcccgcgcg cagcgcaccg agcagcgaga
      121 acttgttgga cgacgaccat cccgccagca tcgcgccgat gatcgtcatg ccgccgaagg
      181 caaagaccat cagcagcccc gcatctagcc gtgcgatctg catcggatag ctcttgtcgc
      241 cgaaccagtc cgccagcatc ggtatgagat gtccgggcac cagcacgccg ccgaacggga
      301 tcacggcgaa caccagtagt accggcgtga acacgaccca gggggcgacc gcgtacgcgt
      361 accagtcgta ggtcttgggc ttgaagtcct ccttcaggat catcttcagg ccgtcggcca
      421 tgccgtggaa caggccccac cagaccagct tcacctgcgt gaacgggatg cggaagtagg
      481 cacggttggc accgatgcgg tccgacatca cggcggcctg cttgcgctcg acccaggtca
      541 ggatcgtgcc gaagcccatc agcaccgaaa tggcgtaggt gatgaagacg atggagatga
      601 cgagatcctg gatcatgcga tcgcctcgct ccgtgcggcc agctgcgcga acagctgctc
      661 ggcgtgcacc acaccgtcct tcttgcggac gatcggctca aagcggctca ccacgccggc
      721 gaagttcgtg tagtgcccgg cgcgctcggt ctggatgctc accggaatga agacatccgc
      781 gtggccgttt tccggcgcca ggtaggaacc gagcaggatc accttcgcgc cacggggcag
      841 atcggcgaag ctcgcgcctt cgccccagac gagcacgagg tcggactcgg ccggcaccag
      901 gtgcgcttcg gcgccgaaca gctcctgtgc cctacgcgtg ttcgggttct tgtcggcacg
      961 gatcagcagg tcatcttcca gtcgctcacc cggttgcgcc tgccagtcgg acttgacgat
     1021 gcactcgacg cggtcgccga gcgttgcctt gaatgccgcc agctcctcgt tcgagcccca
     1081 gctcgacacc agcgcggccg gccgcttcgc ttctccaatg aggcgccgcg cagccgcaat
     1141 cgcgtcaccg agtgcggctg gcttgccctt ctgcatcgcc tgttcagcgc gggggcgctc
     1201 gaagatccgg gcgagatcgc ggcccttgtt gcagatccag ggcccgttga cctccgggtt
     1261 ctcgcgcggc gtcacgcggg cgatggcggt gttttcgcgc gggtcgagcg cgttgagctt
     1321 ccactcggcc ttgcggtgcc acagatccac ggtgcatccg cggctgcagc ccgggcacac
     1381 cgaaggcgtg gctttcagat accagacgcg cgccttgtgc aggaactgtt tcgaaagcag
     1441 cgccccgacc gggcagatgt cgatgacatt gtcggagtac gggtcggact cgaagtcgcg
     1501 gctttcgacc ggcctcacca gggatgcgtc gccgcggttc tggatgccaa gcgcgtgcga
     1561 tttggaaatc tcgcgcgtga agcgcacgca acgtgagcag acgatgcagc gctcgttgtc
     1621 gagcacgatc cggctcgaca gttcgaagta cttcgtcgca tggaccttcg gcacggtcga
     1681 taccgagggc agcccgttca tttcgtagtg gtagtcctgc agcgtgcact cgccggcctt
     1741 gtcgcagatg ccgcagtcga ccgggtggtt caacaggatg aactgcagcg tctcgcggcg
     1801 ccgcgcccgc accgcctcgg agtccgtcag cacccgcatg ccctcggtga ccggcatgtt
     1861 gcaggcgatc tccatccagc tgctgccgcc cccttcgcct tcgacctcga ccacacagat
     1921 acggcagttg cccgagatcg acagattggg atgccagcag aaggtcggaa tgaagaagcc
     1981 agcggccttt gccgcctgca tgaccgtctg gcccttggcc gcggtgaccg gctgcccgtt
     2041 gatgaagaag tcaggcatgc gcggcctcct gctcggcaac g
//
LOCUS       DAOMFD010000299         2060 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_32962_length_2060_cov_26.907232, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000299 DAOMFD010000000
VERSION     DAOMFD010000299.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2060)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2060
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32962_length_2060_cov_26.907232"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="PL152_11200"
     CDS             <1..236
                     /locus_tag="PL152_11200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49895.1"
                     /translation="YKRERAANASGTLFRRDYAEALYAYALAQPGDAAGREARQAALT
                     QASELVAGASAEARQTVEMRELAGWIAEARKTS"
     gene            259..1878
                     /locus_tag="PL152_11205"
     CDS             259..1878
                     /locus_tag="PL152_11205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HQR49896.1"
                     /translation="MHGLMQDHPLLISSLIRHAAANHGRREVVSRLGDGSIHRTNYAS
                     VERRARRLARALEMRGIRSGDRVATLAWNTFRHLECFYGVSGLGAVLNTVNPRLFHDQ
                     IEYIVRHAENRVLIFDADLLPLVEAIAPQVPCVERYVVMCDRASMPDSRLQLDAYEDW
                     IAGADDDFQWPDMDERAASSLCYTSGTTGNPKGVLYSHRSTLIHALSASQADCFGLSS
                     LDSVMPIAPMFHANAWSLPYACPMMGAKMVLPGRHMDAASLCELLQDEGVTFSVGVPT
                     IWTMLLAHMAERGVSLPKLRRCVVAGTALPPAVQRALKEQHGVTAIHAWGMTETSPIG
                     TMATPTPEVLALPEDRQAEELLKQGRVLYGVEIEARDVDGGPAPRDGRSFGPLWIRGP
                     WVASGYFKGEGGAVLDANGWFPTGDVATWDEYGFMKITDRTKDVIKSGGEWISSIDVE
                     NAAMSHPKVALAAAVAAFHPKWDERPVLVIVPREGQQPTREEILEFLAPRMAKWWLPD
                     DVVFIREMPLTATGKILKAKLREQYWHHLER"
BASE COUNT          344 a          672 c          729 g          315 t
ORIGIN      
        1 actacaagcg cgaaagggcg gccaatgcga gcgggacgct gttccgccgc gactatgcgg
       61 aagcacttta cgcctacgcg ctggcgcagc ccggcgatgc ggcgggccgc gaggcccgcc
      121 aggcggcgct gacgcaggcg tcggaactgg tcgccggagc gtccgccgaa gcccggcaga
      181 ccgtcgagat gcgcgagctg gccggctgga tcgccgaggc gcgcaagacc tcctgagcat
      241 ctccgcggga gtaccgccat gcacggcctg atgcaggacc atccgctgct gatctcgtcg
      301 ctgatcaggc acgcggcggc aaaccacgga cggcgcgagg tggtgtcgcg actcggcgac
      361 ggcagcatcc accgcacgaa ctacgcgagc gtcgagcgcc gtgcgcggcg cctcgcgcgc
      421 gccctcgaga tgcgcggcat ccgctccggg gaccgcgtgg ccacgctggc ctggaatacc
      481 ttccgtcacc tcgaatgctt ctacggtgtc tccggcctcg gcgccgtgct caacacagtc
      541 aacccgcggt tgttccacga ccagatcgaa tacatcgtgc gtcacgcgga gaatcgcgtg
      601 ctcatcttcg acgccgacct cctgccgctc gtcgaggcga tcgcgccgca ggttccctgc
      661 gtggagcgct acgtcgtgat gtgcgatcgc gcctcgatgc ccgattcgcg gttgcagctc
      721 gacgcctacg aggactggat cgccggcgcc gacgacgact tccagtggcc ggacatggac
      781 gagcgtgccg cctcctcgct ctgctacacg tcgggcacca cgggcaatcc gaagggcgtg
      841 ctctacagcc accgctcgac gctgatccac gccttgagcg catcgcaggc ggactgcttc
      901 gggctgtcct cgcttgacag cgtgatgccg atcgcgccga tgttccacgc caacgcgtgg
      961 tcgctgccct acgcctgccc gatgatgggc gcgaagatgg tgctgcccgg ccggcacatg
     1021 gacgctgcga gcctgtgcga actgctgcag gacgaaggtg tgaccttctc ggtcggggtg
     1081 cccacgatct ggaccatgct gctcgcacac atggcggagc ggggtgtgag cctgccgaag
     1141 ctgcggcgct gcgtcgtggc gggcacggcg ttgccgcccg cggtgcagcg ggcgctgaag
     1201 gaacagcacg gagtgaccgc gatccacgcc tggggcatga cggagacgag tcccatcgga
     1261 acgatggcga ccccgacgcc ggaggtgctc gccctcccgg aggaccggca ggccgaggag
     1321 ttgctgaagc agggccgcgt cctctacgga gtcgagatcg aagcgcgcga cgtggatggc
     1381 ggtcctgcgc cgcgcgacgg ccggagtttc ggcccgctgt ggatccgtgg gccgtgggtc
     1441 gcgtcgggct atttcaaggg tgaaggcggt gcggtgctcg acgcgaacgg ctggttcccg
     1501 accggcgacg tcgccacgtg ggacgagtac gggttcatga agatcaccga ccggaccaag
     1561 gacgtgatca agtccggcgg cgagtggatc agctcgatcg acgtcgagaa cgcggcaatg
     1621 tcgcacccca aggtcgcgct ggccgcggcg gtcgcggcgt tccatccgaa gtgggacgag
     1681 cggccggtgc tggtgatcgt gccgcgggaa ggccagcagc cgacgcgcga ggagatcctg
     1741 gagttcctcg cgccgcgcat ggcgaagtgg tggctgcccg acgacgtggt gttcatccgg
     1801 gagatgccgc tcaccgcgac cggcaagatc ctcaaggcca aactgcgcga gcagtactgg
     1861 catcacctgg aaaggtagat ggggacattc tcctatttcg cgctgccgaa ataggagaat
     1921 gtcccccttt cattcatgcc gcagggcgtc gatcggattc aggcccgcgg cgcggcgcgc
     1981 cgggaagtag ccgaacagca tgccaatcgc ggtggcgaag ccgaatgaca gcaggttgat
     2041 gcccgggctg aagatatagg
//
LOCUS       DAOMFD010000300         2059 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_32998_length_2059_cov_34.314870, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000300 DAOMFD010000000
VERSION     DAOMFD010000300.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32998_length_2059_cov_34.314870"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..432
                     /gene="rpsJ"
                     /locus_tag="PL152_11210"
     CDS             121..432
                     /gene="rpsJ"
                     /locus_tag="PL152_11210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011929568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HQR49897.1"
                     /translation="MARQNIRIRLKAFDHRLIDNSAREIVETAKRTGATVRGPVPLPV
                     KMERFTLLVSPHADKDARDQYELRTHKRLMDILNPTDKTVDALMKLELPAGVDVQIKL
                     N"
     gene            658..1335
                     /gene="rplC"
                     /locus_tag="PL152_11215"
     CDS             658..1335
                     /gene="rplC"
                     /locus_tag="PL152_11215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003103877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HQR49898.1"
                     /translation="MTIGLIGRKAGMTRVFTDAGESIPVTVIEALPNRVTQVKAVEGD
                     GYRAVQVAFGERKASRLSRPLAGHYASAKVAPGESLVEFRLADGEGAELAPGAEIKVD
                     IFAAGQVVDVTGTTVGKGFAGTMKRHNFAGGYASHGASVVHRAPGSIGQRQTPGRVFP
                     GKRMSGHMGSVRRTTENLKVVEVDAARNLLLVRGAVPGAPGGQVIVRPSLKTARRALR
                     KTKGARK"
     gene            1339..1959
                     /gene="rplD"
                     /locus_tag="PL152_11220"
     CDS             1339..1959
                     /gene="rplD"
                     /locus_tag="PL152_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017395329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HQR49899.1"
                     /translation="MATQLKFLTDTAAQKHLEVSEKAFGRDFNEALVHQVVVAYMAAG
                     RAGTKRQKSRAEVRGGGIKPWKQKGSGRARAGSIRSPIWVGGGRAFAARPRDYSQKVN
                     RKMYQGAMRAMLSELARLERLVVVESLALEAPKTRLLAAKLKEYGLQDALILVEAYDE
                     KLDLASRNLPGVTVLPVAAIDPQSLISHGHVVATVGAVRMLEEKLA"
BASE COUNT          340 a          725 c          725 g          269 t
ORIGIN      
        1 gcctgcgctt tgcgatccgc gagggcggac gcacggtggg cgcgggcgtg gtgtccaaga
       61 ttctgaagtg acgtcgcacc agccgacgga cgctttatcg ctctttacag gtaccgacac
      121 atggcacgcc agaacatccg catccgcctc aaggcgttcg accaccggct gatcgacaac
      181 tcggcccggg agatcgtcga gacggccaag cgcacgggcg ccaccgtgcg cggcccggtg
      241 ccgctgccgg tcaagatgga gcgcttcacg ctgctcgtgt cgccgcacgc ggacaaggac
      301 gcgcgcgacc agtacgagct gcgcacccac aagcgcctga tggacatcct gaatcccacc
      361 gacaagacgg tggacgcgct catgaagctc gagctgcccg ccggcgtgga cgtgcagatc
      421 aagctcaact agcccggaac ttgcaatcga ccgagccgcc gctacaatag gcggctctct
      481 cgccgaggcc tggccgggtt gaccggtacc gctcgagcgg cgcgaccccg gacccccgcg
      541 gacgcacctc tcagcccttg acgagcacct gcccgccgat ccgtccaccg ggacgggcgg
      601 gcgagttttt tcgagccccg gccaatcgca gtcggggccg cgtacgagga agaatcgatg
      661 acgatcggtc tgattggccg caaggccggc atgacgcgcg tcttcacgga cgcgggcgaa
      721 tcgatcccgg tgacggtgat cgaggccctg ccgaaccgcg tcacccaggt gaaggccgtc
      781 gagggcgacg gctaccgcgc agtgcaggtc gccttcggcg agcgcaaggc ctcgcgcctc
      841 tcgcgcccgc tggcgggcca ctacgccagc gccaaggtcg ccccgggcga gtcgctcgtg
      901 gagttccgcc tggccgacgg cgagggcgcc gagctcgcgc cgggcgccga gatcaaggtg
      961 gacatcttcg cggccgggca ggtggtcgac gtgaccggca ccaccgtcgg caagggcttc
     1021 gccggcacca tgaagcgtca caacttcgcg ggcggctacg cctcgcacgg cgcctcggtc
     1081 gtccaccgtg ccccgggctc gatcggccag cgccagacgc cgggccgcgt gttcccgggc
     1141 aagcgcatgt ccggccacat gggcagcgtg cggcgcacca ccgagaacct gaaggtcgtc
     1201 gaggtggacg cggcccgcaa cctgctgctg gtccgcggcg ccgtccccgg cgcgccgggc
     1261 gggcaggtca tcgtgcggcc gtcgctcaag accgcgcgcc gggcgctgcg caagaccaag
     1321 ggcgcgagga agtaagccat ggccacgcag ctcaagttcc tcaccgacac cgcggcgcag
     1381 aagcacctcg aggtgtccga gaaggcgttc gggcgcgatt tcaacgaggc gctcgtccac
     1441 caggtcgtgg tggcctacat ggccgccggc cgtgccggca ccaagcgcca gaagagccgc
     1501 gccgaagtgc gcggcggcgg catcaagccg tggaagcaga agggcagcgg ccgcgcccgg
     1561 gcgggctcga tccgcagccc gatctgggtc ggcggcggcc gcgcgttcgc cgcgcgcccc
     1621 cgcgactact cgcagaaggt caaccgcaag atgtaccagg gcgcgatgcg cgcgatgctc
     1681 tccgagctcg cccgcctcga gcggctggtc gtcgtcgaat cgctcgcgct cgaggcgccg
     1741 aagacgcgcc tgctggcggc gaagctcaag gagtacggcc tgcaggatgc cctgatcctg
     1801 gtcgaggcct acgacgagaa gctggacctc gcctcgcgca accttccggg cgtgacggtg
     1861 ctgccggtgg ccgcgatcga cccgcagagc ctcatctcgc acggccacgt cgtggcgacg
     1921 gtgggcgcgg tgcgcatgct cgaggagaag ctcgcatgag cagcaaggaa cgactgatga
     1981 ccgtgctgct cgcgccgcac gtgtcggaga agagcgcgat cgcggccgag aaggccaacc
     2041 aggtcgtgtt ccgcgtgcg
//
LOCUS       DAOMFD010000301         2058 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_33022_length_2058_cov_53.415876, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000301 DAOMFD010000000
VERSION     DAOMFD010000301.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33022_length_2058_cov_53.415876"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="PL152_11225"
     CDS             complement(<1..292)
                     /locus_tag="PL152_11225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49900.1"
                     /translation="MRTLFLHRSWRDRFASTGRVRLRTFAERSVEHLKHWAVVYVAIA
                     AIALWFHAHYGFGLNASPSLPHRLFLIHKGEMPSRGDFVAFRWAGGGPYPAGV"
     gene            complement(310..675)
                     /locus_tag="PL152_11230"
     CDS             complement(310..675)
                     /locus_tag="PL152_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49901.1"
                     /translation="MNARAAALLVGANALVSAALIALVHLWLVPERLPALAVLDVAEL
                     YRLKELQVAAVLVKRDASDEERAGALRRAAGFGTEVSTLLQALPGECRCIVLARGAVM
                     GSEPLLRDLTPDVRRRLGL"
     gene            complement(672..>2058)
                     /locus_tag="PL152_11235"
     CDS             complement(672..>2058)
                     /locus_tag="PL152_11235"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5934 domain-containing protein"
                     /protein_id="HQR49902.1"
                     /translation="ARGFELSNDTMMATQALIASLPMTLSAPFHADLQRMKRVTTKTS
                     ANAVHLAPLVAEWQGTGTPVLLFGGRRGQVMQIDVYDNPAGNYNVAIAGTSGSGKSLL
                     LNEIAAAYLGTGARVWIIDVGRSYEKACRNFGGSFIEFTENAALSLNPFTLVEDIDED
                     MELLQPLLAQMVSPREALDGFRYSTLGAAIKKVWKALGRAMTVTDIHDLLATGRLDED
                     EAGTPPTGEGDRRLKDLAAMLHPYTRDGAYGKYFHAESTIDFSADLIVLELEELKSKK
                     DLQTVVLLIVMYRITREMYFSRDRKKIVIIDEAWDLLSGGATAEFIEAGYRRARKYRG
                     AFMSATQGVDDYYRNPAAKAALDNSDWMFLLRQKPESIEMMDKLGQLTMDDAMKRLLL
                     SLRTEHGAYSEVFIHSPAGNGIGRLIVDPYSLLLFSSRAEDFNAINAKRAAGLDVSAA
                     IDAVLRERGLA"
BASE COUNT          316 a          681 c          732 g          329 t
ORIGIN      
        1 tgacgcccgc cgggtacggt ccgccgccgg cccagcggaa ggcgacgaag tcgcctcggc
       61 tcggcatctc gcccttgtgg atgaggaaga gccggtgcgg cagactgggc gaggcgttca
      121 gcccgaagcc atagtgggcg tgaaaccaga gcgcgatcgc ggcgatcgcg acgtagacga
      181 cggcccagtg cttcaggtgt tcgaccgagc gctcggcgaa cgtgcgcagc cggacccttc
      241 cggtcgatgc gaaacggtcg cgccagcttc gatgcaggaa cagggtgcgc atgcggactc
      301 ccgatgcagc tacaggccga gccggcgccg cacgtccggc gtcaggtctc gcagcagggg
      361 ttcggagccc atcacggcgc cacgcgccag cacgatgcag cggcactcgc ccggcagcgc
      421 ctggagcagc gtgctcacct ccgtgccgaa gcccgcggcg cggcgcagtg cgccggcgcg
      481 ctcttcgtcg ctggcgtcgc gcttgaccag caccgcggcg acctgcagct ccttcaggcg
      541 atagagctcc gccacgtcca gcacggccag cgccggcagg cgctcgggca cgagccacag
      601 gtgaacgagt gcgatcagcg cggcgctcac cagcgcgttg gcgcccacga gcagggcggc
      661 ggcccgtgcg ttcacgcgag cccccgttcg cgcaagaccg cgtcgatggc ggcggagacg
      721 tccaggcccg ccgcgcgctt ggcgttgatg gcgttgaagt cctcggcgcg gctgctgaag
      781 agcagcaggc tgtacggatc gacgatcagc cgtccgatgc cgttgcccgc cggcgagtgg
      841 atgaagacct cggagtacgc gccgtgctcg gtgcgcagcg acagcagcag ccgcttcatc
      901 gcatcgtcca tcgtgagctg accgagcttg tccatcatct cgatcgactc gggcttctgg
      961 cgcagcagga acatccagtc ggagttgtcc agtgccgcct tcgccgccgg attgcggtag
     1021 tagtcgtcca cgccctgcgt ggccgacatg aaggcgccgc ggtacttgcg cgcgcgccgg
     1081 tagccggctt cgatgaactc ggcggtggcg ccgccggaga gcaggtccca ggcctcgtcg
     1141 atgatcacga tcttcttgcg gtcgcgcgag aagtacatct cgcgcgtgat gcgatacatg
     1201 acgatcagca gcaccaccgt ctgcaggtcc ttcttggact tgagctcctc gagctcgagc
     1261 acgatgaggt cggcgctgaa gtcgatggtg gactcggcgt ggaagtactt gccgtaggcg
     1321 ccgtcgcgcg tgtacggatg cagcatcgcg gcgaggtcct tcaggcgtcg gtcgccttcg
     1381 ccggtgggcg gggtgccggc ctcgtcctcg tcgagccgtc cggtcgcgag taggtcgtgg
     1441 atgtcggtga ccgtcatcgc gcggccgagc gccttccaga ccttcttgat cgccgcgccc
     1501 agcgtcgagt agcggaagcc gtcgagcgcc tcgcgcgggg acaccatctg tgcgagcagc
     1561 ggctgcagaa gctccatgtc ctcgtcgatg tcctcgacca gggtgaacgg gttgagcgac
     1621 agcgcggcgt tctcggtgaa ctcgatgaag ctgccgccga agttgcggca cgccttctcg
     1681 taggagcggc cgacgtcgat gatccagacg cgcgcgccgg tgccgagata ggcggcggcg
     1741 atctcgttga gcagcagcga cttgcccgag cccgaggtgc cggcgatcgc cacgttgtag
     1801 ttgccggccg ggttgtcgta gacgtcgatc tgcatcacct ggccgcgtcg accgccgaag
     1861 agtaggaccg gcgtgccggt gccctgccac tcggcgacca gcggagccag gtgcaccgcg
     1921 ttcgcggaag tcttcgtggt gacgcgcttc atgcgctgca ggtccgcgtg gaacggcgcc
     1981 gacagcgtca tcggcagcga tgcgatgagc gcctgggtgg ccatcatcgt gtcgttcgag
     2041 agctcgaagc cgcgcgca
//
LOCUS       DAOMFD010000302         2056 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_33089_length_2056_cov_25.982009, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000302 DAOMFD010000000
VERSION     DAOMFD010000302.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2056)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2056
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33089_length_2056_cov_25.982009"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(99..1874)
                     /locus_tag="PL152_11240"
     CDS             complement(99..1874)
                     /locus_tag="PL152_11240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HQR49903.1"
                     /translation="MTDAPAEREDRGLWQRLRRRKVGQWAIAYVAGAWGFLQGLQYTS
                     GLLQWPEQLQKLAGLALLIGLPIVIVVAWFHGEGGGQRVTRAELAILTLLFLLGGGLF
                     WRFQHAATPVVAADRSSTLPAAGAAPGDHSIAVLPFVNMSPDASQEYFADGIAEELLN
                     LLARIPQLRVISRSSAFSFKGRNLDVTEIARQLHVRYVLEGSVRKAGDQVRISTQLVD
                     ARSDTQLWAETYDRPLDNIFAVQDDIAAAVVSQLKLKLLGAAPTARAIDPKAFALYLQ
                     GRYLMRQGTREGFGRSLELFRQALEIQPDYPAAWDGLARDYTNEVNSGARPIDEGYRL
                     AREAADRALAIDPEFAPAHSRMGVIAMNYDGDLAAAARHFERALQLAPTSTDIIGNAA
                     VLAHNLGRVDESIALGEYVTARDPVNPTAFANLGFSYQNAGRLDDAIASYRTVLALSP
                     DYDGANYATGVALLLKGDAAGGLQALQKEAGGWGEIGLPMAWYAVGDRARSDAALESL
                     IRTKAADSAYNIAYVLAYRGETDRAFEWLEKAAQYRDPGLAEIAVEPLFANLRSDPRW
                     LPFLRRLGMAPEQLAAIEFVVKVPD"
BASE COUNT          323 a          714 c          701 g          318 t
ORIGIN      
        1 aggcgggcaa cgtcaacctc gacccgacgt cgctgccggc gtaccgggcg gcggccgact
       61 acctcattgc gcgcgggaag tgatccggcg gcccgggctc agtcgggaac cttcacgacg
      121 aattcgatgg ccgcgagctg ctccggcgcc atgccgagcc tgcgcaggaa cggcagccac
      181 cgtgggtcgg agcgcaggtt ggcgaacagg ggttcgacgg caatctccgc cagtcccgga
      241 tcgcggtact gggccgcctt ttccagccac tcgaacgccc gatccgtctc gccgcggtac
      301 gcgagcacgt aggcgatgtt gtaggccgag tcggccgcct tggtgcgtat caacgattcg
      361 agcgccgcgt ccgatcgtgc ccggtcgcca acggcatacc aagccatcgg caggccgatc
      421 tcgccccagc ccccggcttc cttctgcaac gcctgcagcc cgccggcggc gtcgcccttc
      481 agcagcagcg ccacgccggt cgcgtaattc gccccgtcgt agtccggact cagggcgagc
      541 accgtgcgat agctggcaat ggcgtcgtcg aggcgtccgg cgttctgata ggaaaaaccc
      601 aggttggcga atgcggtcgg attgaccgga tcgcgcgccg tgacgtactc gccgagcgcg
      661 atgctctcgt cgacccggcc gaggttgtgc gcgagcaccg ccgcattgcc gatgatgtcg
      721 gtgctggtcg gggccagctg caatgcccgc tcgaagtgtc gcgcggcggc cgcgaggtcc
      781 ccgtcgtagt tcatggcgat cacacccatg cgcgagtgcg ccggcgcgaa ctcggggtcg
      841 atcgcgagcg cgcgatccgc ggcctcgcgt gccagccggt agccctcgtc gatcgggcgt
      901 gcgccgctgt tgacctcgtt ggtgtagtcc cgggcaagtc catcccacgc ggccggatag
      961 tcgggctgga tctcgagcgc ctgcctgaac aattccagcg atcgtccgaa accctcccgc
     1021 gtcccctgtc gcatcaggta acgaccctgc aggtacagcg caaaggcctt cggatcgatg
     1081 gcccgcgccg tcggcgcggc gccgagcagc ttgagcttga gttgcgagac caccgcagcc
     1141 gcgatgtcgt cctggacggc gaagatgttg tcgagtggcc ggtcgtaggt ctcggcccac
     1201 aactgggtgt cggaacgcgc gtcgaccagc tgcgtgctga tccggacctg gtcgccggcc
     1261 ttgcgcaccg aaccttccag gacgtagcgc acgtgcagct ggcgggcaat ctccgtgacg
     1321 tcgaggttcc tgcctttgaa cgagaacgcg gacgatcggg agatcacccg cagttgcggg
     1381 atcctcgcca gcaggttgag cagttcctcg gcgattccgt cggcgaagta ctcctggctc
     1441 gcatcggggc tcatgttcac gaacggaagc acggcgatgg agtggtcccc gggagcggcg
     1501 ccggccgcgg gcagcgtcga gctccgatcg gcagccacca ccggcgtggc cgcgtgctgg
     1561 aagcgccaga acagcccgcc accgagcagg aacaacaggg tcaggatcgc gagctcggca
     1621 cgcgtgacgc gctggccgcc accctcgccg tgaaaccagg cgaccacgat cacgatcggc
     1681 aggccgatca gcaaggcgag gccggcgagc ttctgcaact gttccggcca ctgcagcaga
     1741 ccgctggtgt actgcaggcc ctgcaggaat ccccacgcgc cggcgacata ggcgatcgcc
     1801 cactgtccga ccttgcggcg gcgcaggcgc tgccacagtc cccggtcctc acgctctgcc
     1861 ggcgcgtcgg tcacggctgc ctccccgatg cgcccagtgt agcgaaatat ggggggtggc
     1921 cggcgtcgcc gggatcggcg ctgtcagcgg cgggccgcgt gcgcccgcac taccgggagc
     1981 gctgcagccg tgggcaatcc cgcgcctatt tcgggaacat gaactggacc tggacgcgca
     2041 actgccagtc cgcgcc
//
LOCUS       DAOMFD010000303         2053 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_33169_length_2053_cov_33.372372, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000303 DAOMFD010000000
VERSION     DAOMFD010000303.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33169_length_2053_cov_33.372372"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..879
                     /locus_tag="PL152_11245"
     CDS             <1..879
                     /locus_tag="PL152_11245"
                     /inference="COORDINATES: protein motif:HMM:NF021391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2092 domain-containing protein"
                     /protein_id="HQR49904.1"
                     /translation="HDLARLRLASDRGRHEAAEERIVSMSGSMIHRFGRGEHPMESNL
                     RSPLTARFAQCCALLALATASVAAQAQSAGVAPEAKQILKASTDFLASQQRFSADTRN
                     TLEIVLKSGQKIEFNSTGRQSVQRPNKLRSERSGDLVEQLFVYDGKSLTLSQPQDKVY
                     AQVAAPGTLEEMLDFARIKYDVVAPLGDMIFKNAYDILMDGVTEGIVVGQAVIEGVLC
                     DHLAFRAPDVDWQIWIQQGAQPLPRRIVVTTLDLPNAPQFAATVTRWNLEPSFDAQTF
                     TFTPQAGMKQIELLPR"
     gene            896..1372
                     /locus_tag="PL152_11250"
     CDS             896..1372
                     /locus_tag="PL152_11250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49905.1"
                     /translation="MSKNRNLKALAVTTALPLLFVAGVSPDAPGALTLLQDAGAVVGA
                     PLTPASAAGVARRTTRRVVVAESASTQQQQAATAQQQSATAQQQSATAQQQSATAQQQ
                     AAAAARPPGAPPVGTVVSALPAGCKPEAKGGVEYQNCGGVYYRAAFQGNNLVYVVQ"
     gene            1419..1736
                     /locus_tag="PL152_11255"
     CDS             1419..1736
                     /locus_tag="PL152_11255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49906.1"
                     /translation="MKHGITHRTRAVALATIMIAGFAGTAMAVDPGVGVGKPGVGVVP
                     GAGVGAPGVGTRDPGINQPGVAGNVGTPGVGAPGIGVVDPGLNQPGVAGNVGGVARRS
                     VRR"
BASE COUNT          358 a          672 c          685 g          338 t
ORIGIN      
        1 catgatctcg ctcgtctccg gctggcatcc gatcgtggaa ggcatgaagc ggctgaagag
       61 cgaatagttt ccatgagcgg cagcatgatc catcgattcg gcagaggaga acatcccatg
      121 gaatccaacc ttcgcagtcc cctgacggcg cggtttgcgc agtgctgcgc gcttctggca
      181 ctggcgacgg cgtcggtggc cgcacaggcc cagtccgcgg gtgttgcgcc cgaggcgaag
      241 cagatcctca aggcatccac tgacttcctg gcaagccagc aacggttcag tgccgatacc
      301 cgcaacacac tcgagatcgt gctcaagtcc gggcagaaga tcgagttcaa cagcacgggc
      361 cggcagtccg tgcagaggcc gaacaagctg cgctcggagc gcagcggcga tctcgtcgag
      421 cagctgttcg tctacgacgg caagtcgctg acgctttccc aaccgcagga caaggtctac
      481 gcccaggtcg cggcccccgg cacgctggag gaaatgctcg atttcgcgcg catcaaatac
      541 gatgtcgtcg cgccgttagg tgacatgatc ttcaagaatg cgtacgacat cctgatggac
      601 ggtgtcaccg aaggcatcgt ggtcggccag gcggtgatcg agggggtgct gtgcgatcac
      661 ctcgcgtttc gcgcgccgga cgtcgattgg cagatctgga tccagcaggg agcgcagccc
      721 ctgccgcgaa ggatcgtggt caccacgctc gacctgccga atgcgccgca gttcgcggcc
      781 acggtgaccc ggtggaacct cgagccttcg ttcgacgcgc aaacattcac gttcacgccg
      841 caggcgggca tgaagcagat cgagcttctg ccgcgttgat ccagaggcaa ctcacatgag
      901 caagaaccgg aacctcaagg cgctcgccgt cacaacggcg ctgccgctgc tgttcgtggc
      961 cggggtctcg cccgatgcac cgggtgcatt gactctgctg caggacgcgg gcgccgtggt
     1021 gggggctccg ctcacgccgg cttccgcggc cggcgtggct cgacgcacga cccggcgcgt
     1081 cgtcgtggcc gagtcggcct ccacccagca gcaacaggcg gccacagccc aacagcagtc
     1141 ggccacggcg cagcagcagt cggccaccgc acaacaacag tcggcgacgg cgcagcaaca
     1201 ggctgccgct gcagcgcgac ccccgggtgc gcctccggtg ggcactgtgg tcagtgcgct
     1261 tcccgcaggt tgcaagcccg aggccaaggg cggtgtcgag taccagaact gcggcggcgt
     1321 ctactatcgc gcagctttcc agggcaacaa cctggtctac gtggtccagt gatctccgtc
     1381 gaagcggggg ctcgcggcgt catgcaggag aatccgacat gaaacatgga atcacgcatc
     1441 ggacgcgagc ggttgcgctc gccacgatca tgatcgcggg ctttgccggc acggccatgg
     1501 cggtcgatcc gggcgtcggc gtcggcaagc cgggcgtcgg cgtggtgccc ggcgccgggg
     1561 tgggcgcacc gggtgttggc actcgcgacc caggcattaa ccagcccggt gttgccggca
     1621 atgtcggtac gccaggtgta ggcgcgccag gcatcggcgt cgtcgacccg ggcctgaacc
     1681 agccgggcgt ggcgggcaac gtcggcggcg tagcccggcg ttcggtccgg cgctgaacgg
     1741 ggcgtccggc cggatccggt tgagaatcag gccgcttctg gccgatttta cgcctacacc
     1801 agtgtccgct ctccctcgga tcagcgccta gcgagataag agaatcggcg agtcgttcac
     1861 ggagtccgtg agccgcagtc ggcggtgtta tgccggccga tccataggca gcccaaacaa
     1921 ctcccgatcc gtcgacgctg tgctttccct gctccgctgc ggaccggcca gcacgtcagt
     1981 gtgctggcgt tcgcgcgggc cgcctgacat tcagtcaggc cggcaatgat caccgcgctt
     2041 acccttccgt gtg
//
LOCUS       DAOMFD010000304         2037 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_33615_length_2037_cov_33.953582, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000304 DAOMFD010000000
VERSION     DAOMFD010000304.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2037)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2037
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33615_length_2037_cov_33.953582"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..459)
                     /locus_tag="PL152_11260"
     CDS             complement(<1..459)
                     /locus_tag="PL152_11260"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019692099.1"
                     /note="catalyzes the carboxylation of acetyl-CoA to
                     malonyl-CoA; forms a tetramer composed of two alpha (AccA)
                     and two beta (AccD) subunits; one of the two catalytic
                     subunits that can form the acetyl CoA carboxylase enzyme
                     together with a carrier protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HQR49907.1"
                     /translation="MPLSFLEFEQPIAELEAKIDELRFVSSDAEVNIGEEIARLRAKS
                     RALTTSIFANLTPWQVAQLARHPQRPYTLDYAAAIFEDFQELHGDRMYADDLAIVGGL
                     ARLGGRPVMLIGHQKGRDTKERVRRNYGMPKPEGYRKALRLMKLAERFRLP"
     gene            complement(733..>2037)
                     /locus_tag="PL152_11265"
     CDS             complement(733..>2037)
                     /locus_tag="PL152_11265"
                     /inference="COORDINATES: protein motif:HMM:NF025930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HQR49908.1"
                     /translation="HIFDLMEKFAGYGFNKSHSAAYAVLSYQTAWLKAHYPAAFMAAV
                     LSSDMDKTDKVVTIIDECNRMALAVEPPDVNASQYMFTVSGPKSIRYGLGAIKGVGQA
                     AVETMLAERTAHGRFKDLRDLCRRLDLNRVNRRVLEALVRSGALDSFGENRATLMHQL
                     PAAMQAADQSSRAKAAGQDDLFGLAESAPAQADETGESGERLPDWSEAVRLAGERDTL
                     GLYLTGHPITEYERELKPIVSGRIADIGGARPVGSNEGGWRGPGRSVTVAGLVLEVRK
                     RGGRTSFVLDDRSGRLEVTMFEDVYQQYRALVARDAILVVDGSLRWDDFIEDWRLAAK
                     RILDVDQAREQYARRVVLRWPRGEKSGDGRRIVSQIEQALRPSRGGRCSVSIRYARSD
                     AAAFLNFGEEWTVRPSRDLVERLGQILGRDAVEIVYAPRAEG"
BASE COUNT          279 a          757 c          689 g          312 t
ORIGIN      
        1 cggcaggcgg aaccgctcgg ccagcttcat gagcctgagc gccttgcgat agccctcggg
       61 cttcggcatg ccgtaattgc ggcgcacgcg ctccttggtg tcgcggccct tctggtggcc
      121 gatcagcatc accggccggc cgccgaggcg cgcgagtccg ccgacgatcg ccaggtcgtc
      181 cgcgtacatg cggtcgccgt gcaattcctg gaagtcctcg aagatcgccg cggcgtagtc
      241 cagcgtgtag gggcgctgcg gatgccgtgc gagctgcgcg acctgccagg gcgtcaggtt
      301 cgcgaagatg ctggtcgtga gcgcgcggct cttggcgcgc agcctcgcga tctcctcgcc
      361 gatgttgacc tccgcgtccg acgacacgaa gcgcagctcg tcgatcttcg cctcgagctc
      421 ggcgatcggc tgctcgaatt ccaggaagct cagcggcatg ggggcgcagg atgccgcacc
      481 ctgcgcgccc gggcaaatgc tgccggcgcg gcgcgtggac cctctggcgc gcgggcagcg
      541 gcacgcggcc acaaggtccg tgggcatccg gcacttggac gaagggtgcg gtcgggccgg
      601 gcgtcgcgcg gcatggacgc cgcgcgcgag cccccatgga tgggttcacg gcgtcccggc
      661 ccgaccgcac cctttggccg agtgctcacg tgtgaattgg gcgccgaggg ccggtccgcc
      721 cgggcgcgcg gctcagccct cggcacgcgg tgcgtacacg atctcgacgg cgtcgcgccc
      781 gaggatctgc ccgagtcgct ccacgagatc ccgcgacggc cgcacggtcc actcctcgcc
      841 gaaattgagg aacgccgccg cgtcgctgcg cgcgtagcgg atggacaccg agcagcgccc
      901 gccccggctc ggccgcaacg cctgctcgat ctggctcacg atcctgcggc cgtcgccgga
      961 cttctcgccc cgcggccagc gcaatacgac acgccgcgcg tactgctcgc gcgcctggtc
     1021 gacgtcgagg atgcgcttgg cggcgaggcg ccagtcctcg atgaaatcgt cccagcgcag
     1081 gctgccgtcc acgaccagga tcgcgtcccg ggcaaccagc gcgcggtact gctggtacac
     1141 gtcctcgaac atcgtgacct cgaggcgccc gctgcggtcg tccagcacga agctcgtccg
     1201 accgcctcgc ttgcgcacct cgagcaccag cccggccacc gtcacgctcc gccccgggcc
     1261 gcgccaccct ccctcgttcg acccgaccgg ccgcgccccg ccgatgtcgg cgatgcggcc
     1321 gctgacgatc ggcttcagct cacgctcgta ctcggtgatc gggtgaccgg tcaggtacag
     1381 ccccagcgtg tcgcgctcgc cggcgagtcg cacggcttcg ctccagtccg gcaggcgttc
     1441 gccggattcg ccggtctcgt cggcctgcgc cggcgcggac tccgcgagcc cgaacaggtc
     1501 gtcctgcccc gccgccttcg cgcgcgacga ctggtcggcc gcctgcatcg ccgcgggcaa
     1561 ctggtgcatc aacgtcgcgc gattctcccc gaaggaatcg agcgcgcccg agcgcaccag
     1621 cgcctcgagc acgcggcgat tgacgcgatt caggtccagc cgccggcaca ggtcgcggag
     1681 gtccttgaat cggccgtggg cggtgcgctc ggcgagcatg gtctcgaccg ccgcctggcc
     1741 gacgcccttg atcgcaccca gtccgtagcg gatcgacttc gggccggaca ccgtgaacat
     1801 gtactgcgac gcgttcacgt cgggcggctc caccgccagc gccatgcggt tgcactcgtc
     1861 gatgatggtc accaccttgt ccgtcttgtc catgtcggac gacagcaccg ccgccatgaa
     1921 cgccgccggg tagtgcgcct tgagccacgc cgtctggtac gacagcacgg cgtacgccgc
     1981 ggagtgcgac ttgttgaagc cgtagcccgc gaacttctcc atgaggtcga agatgtg
//
LOCUS       DAOMFD010000305         2036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_33647_length_2036_cov_24.209490, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000305 DAOMFD010000000
VERSION     DAOMFD010000305.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33647_length_2036_cov_24.209490"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="PL152_11270"
     CDS             <1..1148
                     /locus_tag="PL152_11270"
                     /inference="COORDINATES: protein motif:HMM:NF012935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HQR49909.1"
                     /translation="PYPMPPLPVKSSGVLLEQGARKLGLHPFPAPMAIASKPYRGRPA
                     CAHCGFCIGFGCEMQAKSSTLYTAIPDAEATGRCEVRSESYVFRVDTNERNRATGVAY
                     FDRDGREHFQKARAVVLCANGAETPRLLLMSAGPRHPDGLANSSGLVGRNLMFNTYNE
                     VGAIFEHELNEYKSVQVTRIVHDFYLTDPKRGFYGGGGIDGRMGPSPIGWPLFSTPPD
                     RNWGAEFKALLEAMPRAMVSATHGTSLPMESNRIDLDPDLKDAWGRPCIRVTYKDHPD
                     DLANTRYLQDRAEEILQAAGAQQTWKMPVTESTLGVHLLGTCRMGNDPKSSVVDRYHR
                     SHDVRNLFICDGSSLVTGGRGQPTMTIQALAFRAGDHIARCAKRGEI"
     gene            complement(1171..>2036)
                     /locus_tag="PL152_11275"
     CDS             complement(1171..>2036)
                     /locus_tag="PL152_11275"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR49910.1"
                     /translation="WTLMVDRMDDERARRLFGIVAAGGTTGGLVAPLATDLLIVPIGR
                     TGILVVAGCLFGIAILCQSLLLRHLRREPAAVRAGQERPIGGNPFAGITLVLRSPYLL
                     AFCAFVLLLASCTTFLYFEQLRVVTDAFPDEDQRTQVFARIDFTVQALTLATQVLFTG
                     AIARRLGVAVLLCSVPIAMVGGFVALAVVGNMWVLAAVMIVRRAGEYALTRPGREILF
                     TSVDTEAKYKAKNLIDVVVYRGGDALSAQLRGGLRASEMSSAALAMVGAGIAAVWALV
                     GYWLARRHSRG"
BASE COUNT          372 a          721 c          656 g          287 t
ORIGIN      
        1 agccctatcc gatgccgccg ctgccggtga aatcctcggg cgtgctgctc gagcaaggcg
       61 cgcgcaagct cggcctgcat ccgttcccgg ccccgatggc gatcgcatcc aaaccgtatc
      121 gcggccgccc tgcctgcgcg cactgtggct tctgcatcgg cttcggctgc gagatgcagg
      181 ccaagtcctc gacgctctac acggcgatcc ccgacgcgga ggcgaccggc cgctgcgaag
      241 tccgcagcga aagctacgtc ttccgggtcg acaccaacga gcgcaatcgc gcgacgggcg
      301 ttgcgtattt cgatcgcgac ggccgggagc atttccagaa ggcacgcgcc gtggtgcttt
      361 gcgccaacgg cgccgagact ccacgactgc tgctcatgtc cgccggaccg cgtcatcccg
      421 acgggctggc gaactcgagc ggcctggtgg gcaggaactt gatgttcaac acctacaacg
      481 aggtcggcgc gatcttcgag cacgagttga acgagtacaa gagcgtgcag gtgacgcgca
      541 tcgtgcacga cttctacctc accgacccga agcgcggctt ctacggcggc ggcggcatcg
      601 acggccgcat gggtccgagc cccatcggct ggccgctgtt ctccacgccc ccggaccgta
      661 actggggtgc cgaattcaag gcgctgctcg aggcgatgcc gcgcgccatg gtgtcggcca
      721 cgcacggcac ctcgctcccg atggaatcga accgcatcga cctcgacccc gatctcaagg
      781 acgcatgggg ccgaccctgc atccgcgtga cgtacaagga tcacccggac gacctcgcga
      841 acacgcgcta cctgcaggat cgcgccgaag agatcctgca ggccgcgggc gcgcagcaga
      901 cgtggaagat gccggtcacc gagtcgacgc tcggcgtgca cctgctcgga acctgccgca
      961 tgggcaacga tccgaagtcc tcggtggtgg atcgctacca ccgcagccac gacgtgcgaa
     1021 atctcttcat ctgcgacggc tcgagcctgg tgaccggtgg acggggccag ccgacgatga
     1081 ccatccaggc actcgccttc cgcgcgggcg accacatcgc gcgctgcgcg aaacgcgggg
     1141 aaatctgaca gcggcagcga tgcgatccgc tcagccccgg gaatgacggc gcgcgagcca
     1201 gtaaccgacc agcgcccaga ctgcggcgat tccggccccg accatggcga gggccgcgga
     1261 actcatctcg ctcgcccgca gaccgccgcg cagttgcgcc gagagcgcgt ccccgccgcg
     1321 gtagaccacg acgtcgatca ggttcttggc cttgtacttg gcctcggtgt cgacggatgt
     1381 gaacaggatc tcccgccccg gccgcgtcaa cgcgtattcg ccggcccgtc gcacgatcat
     1441 taccgccgcc agcacccaca tgttgccgac caccgcaagt gccacaaagc cgcccaccat
     1501 tgcgatcggc acggagcaca gcagcacggc cacgccaagc ctgcgcgcga tggcgccggt
     1561 gaagaggacc tgggtcgcga gcgtgagcgc ctgcaccgtg aagtcgatgc gcgcgaagac
     1621 ctgtgtccgc tggtcctcgt cgggaaacgc gtcggtgacg acccgcaact gctcgaaata
     1681 cagaaacgtc gtgcacgacg cgagcagcag gacgaacgcg cagaacgcga gcaggtaggg
     1741 tgatcgcagc accagcgtga tcccggcgaa cgggttccct ccgatcggtc gctcctgacc
     1801 cgcgcgcacg gccgccggct cccgtcgcag gtgccgcagc aacagtgact ggcagaggat
     1861 ggcgatgccg aacaggcagc ccgcgacgac caggatcccc gtacgaccga tgggtacgat
     1921 cagcaggtcg gtggcgagcg gcgctacgag accgcccgtc gttccgcccg ccgcgacgat
     1981 tccgaacagt cggcgggcac gctcgtcgtc catgcgatcg accatcaggg tccaga
//
LOCUS       DAOMFD010000306         2035 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_33677_length_2035_cov_37.152525, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000306 DAOMFD010000000
VERSION     DAOMFD010000306.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2035)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2035
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33677_length_2035_cov_37.152525"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..1312
                     /locus_tag="PL152_11280"
     CDS             104..1312
                     /locus_tag="PL152_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HQR49911.1"
                     /translation="MTQTIVIVGASHASVQAVDTLRREGHEGPIVLVGEELHLPYNRP
                     PLSKKFFSGELERERLLLRSPHFYEQARVETKLGFRVTAIDRAAQRLRLCDTSELTYD
                     RLLLCVGGRPRTLDVPGHDLPGIHCLRTIADVDAIRADLAGARRLVVVGAGYIGLEAA
                     ASARHLGLDVTVLEMADRPMNRVVAPEISAFYVRRHEREGVKIHCGETVTAFVGEDRV
                     RAVMCGERSYPADLVIVGVGILPETSLAAAAGLRCENGIRVDETCQTSDPNVWAAGDC
                     TNHPSVRYGRRVRLESVDNAVEQAKVSAMNMCGKPARHEHVPWFWSDQYDVKLQIAGL
                     SDGYDQAVLRGNPDSGSFALYYLAQGELLAVDAVNSPKDFMTGKRWIAERKRPAPDRL
                     ADPACDLKTL"
     gene            1323..>2035
                     /locus_tag="PL152_11285"
     CDS             1323..>2035
                     /locus_tag="PL152_11285"
                     /inference="COORDINATES: protein motif:HMM:NF018827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49912.1"
                     /translation="MTPTTRKTSPALKTDDASRALLATLAQWTGGMSPQAFGGAWINV
                     LSRLALTPGRQAVLARNALKKSFALAQFAGDAVRSGKHAPEAEGTPYASRFSDPAWAK
                     FPFNVMAQTFITASELAREAVKNVPGADPASENIVGFATREGMELLAPDNYLATNPQL
                     IRQTIDENGRNLLRGAKHLAEDVVRTLRGQGPAGVEQYKVGEHVGITRGKVILRTRLM
                     ELIQYSPQTPNVYAEPVLVV"
BASE COUNT          336 a          699 c          714 g          286 t
ORIGIN      
        1 gcggcgccgc tcgcgctcga cctgttcctc gacgacaagg ggccgtatat aaaggacgcc
       61 gtgtcgctca ccggcggcca tccttgaagc agcggacgag tccatgaccc agacgatcgt
      121 catcgtggga gcgagccacg cctcggtgca ggcggtggac acgctgcgcc gcgaaggcca
      181 cgagggcccg atcgtgctgg tgggcgagga gctgcacctg ccgtacaacc gcccgcccct
      241 gtcgaagaag ttcttctcgg gcgagctcga gcgcgagcgc ctgctgctgc gcagccccca
      301 tttctacgag caggcccggg tggagacgaa gctcgggttc cgggtgacgg ccatcgaccg
      361 tgcggcgcag cgattgagac tatgtgatac gtcggaattg acgtacgaca ggctgctgct
      421 gtgcgtcggc ggccgaccac gcacgctcga cgtgcccggc cacgacctcc cgggcatcca
      481 ttgcctgcgc acgatcgcgg acgtcgacgc gatccgggcg gacctcgcgg gagcccggcg
      541 gctggtcgtg gtgggcgccg gctacatcgg gctcgaggcc gccgcgagcg cccggcacct
      601 cgggctcgac gtcacggtgc tcgagatggc ggaccgcccc atgaatcgcg tcgtggcgcc
      661 ggagatctcg gctttctacg tgcgccgcca cgagcgcgag ggcgtgaaga tccattgcgg
      721 ggagacggtc actgcgttcg taggcgagga ccgggtccgc gcggtgatgt gcggcgagcg
      781 gtcgtaccct gcggacctcg tgatcgtcgg cgtcggcatc ctgccggaga ccagcctggc
      841 cgcggcggcc gggctgcgct gcgagaacgg catccgggtc gacgagacct gccagacctc
      901 ggatccgaac gtgtgggccg cgggcgactg caccaaccac ccgagcgtgc gctacggccg
      961 gcgggtgcgg ctcgaatccg tggacaacgc ggtcgagcag gccaaggtct ccgcgatgaa
     1021 catgtgcggc aagccggctc ggcacgagca cgtgccgtgg ttctggtccg accagtacga
     1081 cgtgaagctg cagatcgccg ggctgtccga cggctacgac caggccgtgc tgcgcggcaa
     1141 ccccgattcc ggcagcttcg cgctctatta cctcgcgcag ggcgagctgc tcgccgtcga
     1201 tgcggtgaac agccccaagg atttcatgac cggcaagcgc tggatcgccg aacgcaagcg
     1261 cccggcgccc gaccggctgg ccgacccggc ctgcgacctg aagacgcttt gagggcaccg
     1321 acatgacacc gaccacgcgc aagacctcgc ccgccctcaa gaccgacgac gcctcgcgag
     1381 cgctgctcgc gacgctcgcg cagtggacgg gcggcatgtc tccgcaggca ttcggcggag
     1441 cgtggatcaa cgtgctgtcg cgcctggcgc tgacaccggg gcgacaggcc gtgctggcgc
     1501 gcaatgcgct caagaaatcg ttcgcgctgg ctcagtttgc cggcgacgcg gtgcgcagcg
     1561 gcaagcacgc gcccgaggcg gaaggaactc cttacgccag ccggttcagc gacccggcat
     1621 gggccaaatt ccccttcaac gtgatggcgc agacgttcat cacggcttcc gagctggcac
     1681 gcgaggccgt gaagaatgtc ccgggcgcgg accccgcctc ggagaacatc gtcggattcg
     1741 ccacgcgcga gggcatggag ctgctggcgc ccgacaacta cctcgcgacc aacccgcagc
     1801 tgatccggca gacgatcgac gagaacggcc gcaacctgct gcgcggtgcg aagcacctcg
     1861 ccgaggacgt ggtccgcacg ctgcgcggcc aggggcccgc cggcgtcgag cagtacaagg
     1921 tcggcgagca cgtcggcatc accaggggca aggtgatcct gcggacccgg ctcatggagc
     1981 tgatccagta ctcgccgcag acgccgaacg tctacgccga gccggtgctc gtcgt
//
LOCUS       DAOMFD010000307         2019 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_34138_length_2019_cov_37.036660, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000307 DAOMFD010000000
VERSION     DAOMFD010000307.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34138_length_2019_cov_37.036660"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PL152_11290"
     CDS             complement(<1..383)
                     /locus_tag="PL152_11290"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HQR49913.1"
                     /translation="MAANATKRVYAFEEGDGRDRMLLGGKGANLCEMTRIGLRVPPGF
                     VITTECCLEYLADSQRQLPEGAMDEVRSHMAALERKSGKRFGGGDDPLLVSVRSGSAM
                     SMPGMMDTILNLGLNETTLAALIRRT"
     gene            573..1613
                     /locus_tag="PL152_11295"
     CDS             573..1613
                     /locus_tag="PL152_11295"
                     /inference="COORDINATES: protein motif:HMM:NF014036.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HQR49914.1"
                     /translation="MPANLSPEYKAAADAYRKARDPEEKLAHLREMLSVIPKHKGTDH
                     LQADLKHRIKELDEELAGPKRGGARSGPSLVVRPEGAAQLALVGPPNSGKSLLHARLT
                     HSAAKSGPYPYTTQVPEPGMLKWEDVAFQLVDLPAIAAEHPTPWLGSTLQSADACLLV
                     VDLSDPDCLQHVQSVQAELRERKVRLDTRWPAEADAAESTPAEGVDGLDVFAIRLPTL
                     LIANKADRLDHADTDIEVLQELEEPHFPALLVSAESGQGLSDIGPWLWRALGLVRVYT
                     KTPGKPALHDRPFTLRHGEQTVADVARLVHRDMAKSLKFARVWGRSVVAEGQHVGRDH
                     VLADRDVVELHA"
     gene            1654..>2019
                     /locus_tag="PL152_11300"
     CDS             1654..>2019
                     /locus_tag="PL152_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49915.1"
                     /translation="MIPFWFKLAYTAFVLYVAVVWLKHYGWKNFLWFSDIAFLGAVPA
                     MWLENAALSSVLAVAVLLPEILWNVDYVGRLVLRRRITGLTDYMFEPERPLLLRGLSL
                     FHVPLPLVLVWLLVAYGYDS"
BASE COUNT          306 a          689 c          699 g          325 t
ORIGIN      
        1 gtgcggcgga tgagcgcggc gagcgtggtc tcgttgagcc cgaggttgag gatcgtgtcc
       61 atcatgcccg gcatggacat cgcggatccc gagcgcacgg acacgagcag cgggtcgtcg
      121 ccgcccccga agcgcttgcc gctcttgcgt tcgagcgcgg ccatgtgcga acgcacttcg
      181 tccatggcgc cctcgggcaa ctggcgctgc gagtcggcga ggtattcgag gcagcactcg
      241 gtggtgatca cgaagccggg gggcaccctg agcccgatcc gggtcatttc gcagaggttg
      301 gcgcccttgc ccccgagcag catccggtcg cggccgtcgc cttcctcgaa cgcgtacacg
      361 cgcttcgtcg cattggctgc catggtcgat cctccggcgc gcccgggcgg cgtgctcacg
      421 gccaaaaaat accgccgcgc tgccccggcc gaaatacggg aaattcgccg gcggccgctc
      481 gcggtttgcc acgttgcaat ccggtcccga gcgggtacat atgtgaggcg atcgtcggcc
      541 cgacgggccg gtcaccgctg cggatcccac ggatgcccgc gaatctttcg cccgaataca
      601 aggctgcggc cgatgcgtac cgcaaggcgc gcgatccgga ggagaagctc gcgcacctgc
      661 gcgagatgct gagcgtcatc ccgaagcaca agggcacgga tcacctgcag gcggacctca
      721 agcatcggat caaggaactc gacgaggaac tggctggacc gaagcgggga ggcgcacgca
      781 gcgggccatc gctcgtggtg cggcccgagg gcgccgcgca gctcgcgctg gtcgggccgc
      841 cgaacagcgg caagtccctg ctgcacgcgc ggctgacgca ctcggcggcg aagagcggcc
      901 cttatccgta caccacgcag gtccccgagc ccggcatgct caagtgggag gacgtagcct
      961 ttcagctcgt cgacctgccg gcgatcgctg cggagcatcc cacaccgtgg ctcggcagca
     1021 cgctgcagtc ggcggatgcg tgcctgctcg tcgtggacct gagcgatccc gactgcctgc
     1081 agcacgtgca gtcggtccag gccgaactca gggagcgcaa ggtccgcctg gacacccggt
     1141 ggccagcgga ggccgacgct gccgagtcca cgcccgccga aggcgtggac ggcctcgacg
     1201 tgttcgcgat tcgcttgccg acgctgctga tcgcgaacaa ggccgaccgg ctcgaccacg
     1261 ccgacaccga catcgaggtc ctgcaggaac tcgaggagcc gcatttcccg gcgttgctcg
     1321 tctccgcgga gagcgggcag ggtctgtccg acatcggccc ctggttgtgg cgagcgctcg
     1381 gcctcgtgcg cgtgtacacc aagacgcccg gcaagccggc gctgcacgac cggccattca
     1441 cgctgcgtca cggcgagcag acggtcgccg acgtggcgcg cctggtgcac cgggacatgg
     1501 cgaaatcgtt gaagttcgcg cgcgtctggg gtcgttcggt cgtggcggaa ggccagcacg
     1561 tgggccgcga ccacgtgctc gcggaccgcg acgtggtcga actgcacgcc tgaagcgcgt
     1621 cccgcgaccc ggcaggcctg ctacgatccg ggcatgatcc cgttctggtt caagctcgcc
     1681 tacacggcgt tcgtcctgta cgtcgccgtc gtctggctga agcattacgg gtggaagaac
     1741 ttcctgtggt tctccgacat cgcgttcctc ggcgcggtgc ccgcgatgtg gctcgagaat
     1801 gccgcgctgt cgagcgtgct tgcggtcgcg gtgctgctgc ccgagatcct gtggaacgtc
     1861 gactacgtcg ggcggctcgt gctcaggcgc cgcatcaccg ggctcaccga ctacatgttc
     1921 gaacccgagc gaccgctgct gctgcgcggg ctgtcgctgt tccacgtgcc gctgccgctc
     1981 gtgctcgtct ggctgctggt cgcgtacggg tacgactcg
//
LOCUS       DAOMFD010000308         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_34386_length_2010_cov_35.069565, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000308 DAOMFD010000000
VERSION     DAOMFD010000308.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34386_length_2010_cov_35.069565"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /gene="prfC"
                     /locus_tag="PL152_11305"
     CDS             <1..387
                     /gene="prfC"
                     /locus_tag="PL152_11305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650533.1"
                     /note="stimulates the release of release factors 1 and 2
                     from the ribosome after hydrolysis of the ester bond in
                     peptidyl-tRNA has occurred; GDP/GTP-binding protein;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HQR49916.1"
                     /translation="LRMKALQKGLDQLCEEGATQLFRPLRNNDLILGAVGQLQFDVVA
                     FRLQDEYSVQATWDAVNVYTARWVYCDDPKKLAEFRAKAHDNLALDHSGALVYLAPTR
                     VNLQLTEERWPDITFRETREHLASAA"
     gene            362..1642
                     /locus_tag="PL152_11310"
     CDS             362..1642
                     /locus_tag="PL152_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HQR49917.1"
                     /translation="MSTSHPRRSVLARPPVVGWALYDWANSAFATTVMAGFFPVFFKQ
                     YWNAGVAATESTFRLGVTSGIASLVVALMAPAMGAIADRVGARVRLMMVFTVLGSVAT
                     LALATVGQGDWFGAAVLYLVASLGFWGGIVFNDSLLLHVAEPAEYDLVSGYGYALGYL
                     GGGLLFAANVLMTLQPGWFGLSSAAEAVRWSFVSVGVWWFVFALPCAIFVRERRGPAA
                     RVAVATAVKQGFAELKGTLHEISRYRPLLWFLAAYILYIDGVNTVIKMAVDYGLSLGF
                     DQSYLVKALLLTQFVAFPAALAFGWLGGKIGARAGIFIGLAVYLGATCYAYFMDEARD
                     FYSLAVVIGLVQGGVQSLSRSYYGSLVPPDKSSEFFGFYNMMGKASAIIGPALVGVTA
                     AVTHDSRLSILSIVVLFLAGGALLVVATRQGPRE"
     gene            1661..>2010
                     /locus_tag="PL152_11315"
     CDS             1661..>2010
                     /locus_tag="PL152_11315"
                     /inference="COORDINATES: protein motif:HMM:NF014215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HQR49918.1"
                     /translation="MNLSRLSVAIICGFAFLQFSSMHQASAASAQSAAGQELQEPPAP
                     EVDLQWGLRIPMRDGVQLNGTVYRPHGQTERLPVIFTLTPYIADSYHARAMYFAQHGY
                     VFVLVDVRGRGNSGG"
BASE COUNT          274 a          642 c          708 g          386 t
ORIGIN      
        1 ctgcgcatga aggcgctgca gaaggggctc gaccagctct gcgaggaggg cgccacgcag
       61 ctgttccgcc cgctgcgcaa caacgacctc attctcggcg cggtcggtca gctgcagttc
      121 gacgtcgtgg ctttccgcct gcaggacgaa tactcggtgc aggcgacctg ggatgcagtg
      181 aacgtgtaca cggcacgctg ggtgtactgc gacgatccga agaagctcgc ggagttccgc
      241 gccaaggccc acgacaatct cgcgctggat cactccggcg cgctcgtgta cctggcgccc
      301 acgcgcgtca acctgcagct gaccgaggaa cgatggcccg acatcacgtt ccgggagact
      361 cgtgagcacc tcgcatccgc ggcgtagcgt cctcgcacgt ccgccggtcg tcgggtgggc
      421 gctctacgac tgggcgaact cggcgttcgc gaccacggtc atggccgggt tcttcccggt
      481 cttcttcaag cagtactgga acgcgggcgt cgcggcgacc gagagcacct tccgcctcgg
      541 ggtgacgagc gggattgcga gcctggtcgt cgcgctgatg gcgcccgcga tgggcgcgat
      601 cgccgaccgg gtgggcgccc gggtgcggct gatgatggtc ttcacggtgc ttggatcggt
      661 cgcgacgctc gcgctcgcca cggtcggaca gggcgactgg ttcggggcgg cggtgctgta
      721 cctcgtggcg tcgctcggct tctggggcgg gatcgtgttc aacgattcgc tcctgctgca
      781 cgtcgcggaa ccagccgagt atgacctcgt gtccggctac gggtatgccc tggggtacct
      841 cggcggcggc ctgctgttcg ccgccaatgt gctcatgacg ctgcagcccg gctggttcgg
      901 actgtcgagt gccgcggaag cggtgcgctg gtccttcgtg agcgtcggcg tgtggtggtt
      961 cgtattcgcg ctgccgtgcg cgatcttcgt gcgcgagcgc cgcggccccg cagctcgcgt
     1021 ggcggtcgcc acggcggtga agcagggctt cgcggagctc aaggggacat tgcacgagat
     1081 ctcgcggtac cgcccgctgt tgtggttcct cgccgcctac atcctgtaca tcgacggcgt
     1141 gaacacggtg atcaagatgg ccgtggacta cgggctgtcg ctcggcttcg accagtccta
     1201 cctcgtgaag gcgctgttgc tgacgcagtt cgtggccttt cctgcggcgc tcgcgttcgg
     1261 ctggctcggc ggcaagatcg gcgcgcgggc ggggatcttc atcgggctcg cggtttacct
     1321 cggcgcgacc tgctatgcgt acttcatgga cgaggcccgc gacttctact cgctggcggt
     1381 cgtgatcggg ctggtgcagg gcggcgtgca gtcgttgagc cgcagctatt acggcagcct
     1441 cgtgccgccg gacaagtcat ccgagttctt cggtttctac aacatgatgg ggaaggcatc
     1501 ggcgatcatc ggcccggcgc tggtcggggt cacggccgcc gttacgcacg actcgcgcct
     1561 gtcgatcctc tcgatcgtgg tgctgttcct ggccggcggc gccctgctcg tggtggcgac
     1621 gcggcagggt ccgcgtgagt gatgaatggg gggtagacgc atgaacctgt ccagactttc
     1681 cgtcgcgatc atctgcggct tcgcgttcct gcagttttcg tcgatgcatc aggcgtcggc
     1741 tgcaagcgcg cagtcagctg cgggccagga gcttcaggaa ccgcccgcgc ccgaggtcga
     1801 cctgcagtgg ggcctcagga tccccatgcg cgacggcgtg cagctgaacg gcaccgtcta
     1861 ccgaccgcac gggcagacgg agcggctgcc ggtgatcttc acgctgacgc catacatcgc
     1921 ggattcctac cacgcgcgcg ccatgtactt cgcgcagcac ggttacgtgt tcgtgctggt
     1981 cgacgtccgt ggcaggggca attccggcgg
//
LOCUS       DAOMFD010000309         2010 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_34387_length_2010_cov_34.749872, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000309 DAOMFD010000000
VERSION     DAOMFD010000309.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2010)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2010
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34387_length_2010_cov_34.749872"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1098
                     /locus_tag="PL152_11320"
     CDS             <1..1098
                     /locus_tag="PL152_11320"
                     /EC_number="4.1.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-bisphosphate carboxylase large subunit"
                     /protein_id="HQR49919.1"
                     /translation="SIIGNVFSFKPLKAARLEDMRFPVAYVKTFRGPPTGIVVERERL
                     DKYGRPLLGATIKPKLGLSGKNYGRVVYEALLGGLDFTKDDENINSQPFMHWRDRFGF
                     CMEAVQKAQAASGEVKGHYLNITAGTMEEMYKRAEFARDLGSVIVMVDLIVGWTAIQS
                     ISNWARDNDMILHMHRAGHGTYTRQKSHGVSFRVIAKWLRLAGVDHLHAGTAVGKLEG
                     DPLTVQGYYNVCRDSYTKQDLPRGIFFDQDWAGLRKVMPVASGGIHAGQMHQLLDLFG
                     DDVVLQFGGGTIGHPTGIQAGATANRVALEAMVLARNEGRDIRNEGPEILAAAAKTCK
                     PLEAALMTWKDVTFNYASTDTSDFVPTASVA"
     gene            1113..1499
                     /locus_tag="PL152_11325"
     CDS             1113..1499
                     /locus_tag="PL152_11325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose bisphosphate carboxylase small subunit"
                     /protein_id="HQR49920.1"
                     /translation="MRITQGTFSFLADLTDEQIAKQVDYALAQGWAVSLEFTDDPHPR
                     NVYWEMWGLPMFDLKDAAGVIAELRDCRKAHPECYIKLNAFDSTRGFETVRMSFMVHR
                     PKVEARYRLRREEGAGRLVHYTLERA"
     gene            1539..>2010
                     /locus_tag="PL152_11330"
     CDS             1539..>2010
                     /locus_tag="PL152_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007186401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HQR49921.1"
                     /translation="MSTDAAGRTFDLRALLAESGVGAVLDELDRELVGLAPVKLRIRE
                     IASLLVVDKARAELGVAGAKPSLHMCFTGNPGTGKTTVATRMATILHRLGHVRRGHLV
                     AVTRDDLVGQYIGHTAPKTKEVLKRAMGGVLFIDEAYYLYRPENERDYGQEAIEI"
BASE COUNT          350 a          682 c          683 g          295 t
ORIGIN      
        1 tcgatcatcg gcaacgtctt cagcttcaag ccgctcaagg ccgcgcgcct cgaggacatg
       61 cgcttcccgg tcgcctacgt gaagaccttc cgcggaccgc cgacggggat cgtcgtggag
      121 cgcgagcgcc tcgacaagta cggccggccg ctgctcggcg ccacgatcaa gccgaagctg
      181 ggactgtcgg gcaagaacta cggccgcgtc gtctacgagg cgctgctcgg cggcctcgac
      241 ttcaccaagg acgacgagaa catcaactcg cagcccttca tgcactggcg cgaccgcttc
      301 gggttctgca tggaagcggt gcagaaggcg caggccgcga gcggcgaggt gaaggggcat
      361 tacctcaaca tcaccgccgg cacgatggag gagatgtaca agcgcgcgga gttcgcgcgc
      421 gacctgggct ccgtgatcgt gatggtggac ctgatcgtcg gctggacggc catccagtcg
      481 atctccaact gggcccgcga caacgacatg atcctgcaca tgcaccgcgc ggggcacggc
      541 acctacacgc ggcagaagtc gcacggcgtg tcgttccgcg tgatcgcgaa gtggctccgg
      601 ctcgcgggcg tcgaccacct gcacgcgggc accgccgtcg gcaagctcga gggcgacccg
      661 ctcaccgtgc agggctacta caacgtctgc cgcgacagct acacgaagca ggacctgccg
      721 cgcgggatct tcttcgacca ggactgggcg ggcctcagga aggtcatgcc cgtagcgtct
      781 ggcggcatcc acgccggcca gatgcaccag ctgctcgacc tgttcggcga cgacgtcgtg
      841 ctgcagttcg gcggcggcac catcggccac ccgacgggaa tccaggcggg cgccacggcg
      901 aaccgggtcg cgctcgaggc gatggtcctc gcgcgcaacg agggtcgcga catccgcaac
      961 gaggggccgg agatcctcgc ggcggcggcg aagacctgca agccgctcga ggccgcgctc
     1021 atgacctgga aggacgtcac gttcaactat gcctcgaccg acacgtcgga cttcgtgccg
     1081 acggccagcg tcgcctgagt gcggaggtcg ccatgcgcat tactcaagga acgttctcct
     1141 ttctggctga cctgaccgac gagcagatcg ccaagcaggt cgactacgcg ctcgcgcagg
     1201 gctgggcggt gtcgctcgag ttcaccgacg acccgcaccc gcgcaacgtg tactgggaga
     1261 tgtggggcct gccgatgttc gacctgaagg acgccgccgg cgtgatcgcg gaactgcgcg
     1321 actgccgcaa ggcgcacccg gagtgctaca tcaagttgaa cgcgttcgac tccacccgcg
     1381 gcttcgagac ggtacgcatg tcgttcatgg tccaccggcc gaaggtggag gcccgctacc
     1441 ggttgcgtcg cgaagagggc gccggccgcc tcgttcacta cacgctcgag cgggcgtgag
     1501 gagcgcgggg cagcctcctg cttcccggag cccatgacgt gagcacggat gccgccggcc
     1561 ggaccttcga tctccgggcg ctgctcgccg agtccggcgt cggtgccgtg ctcgacgagc
     1621 tcgatcgcga gctcgtcggc ctcgcgccgg tgaagctgcg gatccgcgag atcgcctcgc
     1681 tgctggtggt ggacaaggcg cgcgccgagc tcggagtggc gggcgcgaag ccctcgctgc
     1741 acatgtgctt caccggcaat ccagggaccg gcaagacgac cgtcgccacc cgcatggcga
     1801 ccatactgca tcgcctcggt cacgtgcgca ggggacacct cgtggcggtg acccgcgacg
     1861 acctcgtcgg ccagtacatc ggccacacgg cgccgaagac caaggaagtg ctgaagcgcg
     1921 ccatgggagg cgtgctgttc atcgacgagg cctattacct ctaccggccc gagaacgagc
     1981 gcgactacgg ccaggaggcg atcgagatcc
//
LOCUS       DAOMFD010000310         1998 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_34763_length_1998_cov_33.456511, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000310 DAOMFD010000000
VERSION     DAOMFD010000310.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1998)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1998
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34763_length_1998_cov_33.456511"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1458)
                     /locus_tag="PL152_11335"
     CDS             complement(<1..1458)
                     /locus_tag="PL152_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49922.1"
                     /translation="MLVNVVRVALRKPYTFVVMALLLLIIGPLAAMRTPTDIFPEIRI
                     PVIAIAWQYTGMPVDDMAGRVTTLSQRAITTTVNDVEHIEATTYPGMGIVKIFFHPGV
                     DIATANAQVTAISQTMTRQMPAGMTPPLILNYNASTVPILQLALSGKGLTEQNLFDIG
                     TNQIRSRLITVQGAAVPWPYGGKQRQVQIDLDLAALQSRGLSAVDVANALAAQNVILP
                     VGTQKIGSIEYPILLNNAPDLIDEIGDLPIKVVNGSTIYIRDVAHVHDGNSPQTNIVH
                     VDGDRSVLMSVLKNGTTSTLAIVDGILKKLEEIRPQLPDNIKVVPLNDQSIFVRAAIR
                     GVALEGLIAAALTSVMILLFLGSWRSTLIIAVSIPLSVLGSIIGLAATGETLNIMTLG
                     GLALAVGILVDDATVTIENINWHLEQGKDVETAILDGANQIVTPAFVSLLCICIVFVP
                     MFFLEGVARFLFVPMAEAVMYAMVCSFVLSRTLVPT"
     gene            complement(1449..>1998)
                     /locus_tag="PL152_11340"
     CDS             complement(1449..>1998)
                     /locus_tag="PL152_11340"
                     /inference="COORDINATES: protein motif:HMM:NF024212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HQR49923.1"
                     /translation="IISLDMIGPGAARAAAHPIPGPTTPAPTMDRLANFGFLLKDISR
                     LYSRNFERHAAGHGLTLEQCRVLGHLSRHEGISQARLAYLADMDPMTLGRLLTRMEAS
                     GLIERRAVRGDRRVRALYLRKPAGPVLETIWKLRDLSHAEALAGLSTRQQAQLMAAMN
                     RIHANLDGLLPGVADRGRKRCS"
BASE COUNT          325 a          636 c          697 g          340 t
ORIGIN      
        1 ggtcggcacg agcgtcctcg agagcacgaa cgagcagacc atcgcgtaca tcaccgcctc
       61 ggccatcggc acgaacagga accgtgccac tccctcgagg aagaacatgg gcacgaacac
      121 gatgcagatg cacagcagcg acacgaacgc cggcgtcacg atctggttgg cgccgtccag
      181 gatggccgtc tcgacgtcct tgccctgctc gaggtgccag ttgatgttct cgatggtgac
      241 ggtcgcgtcg tcgacgagga tgccgaccgc gagcgccagg ccacccagcg tcatgatgtt
      301 gagggtctcg ccggtcgccg cgaggccgat gatcgagccc aggacggaca gcgggatcga
      361 cacggcgatg atgagcgtcg agcgccagct gccgaggaac agcaggatca tcacgctggt
      421 gagggcggcg gcgatcagcc cctcgagcgc caccccgcgg atcgccgcgc ggacgaagat
      481 cgactggtcg ttgagcggca ccaccttgat gttgtcgggc agctgcgggc ggatctcctc
      541 gagcttcttc aggatgccgt ccacgatcgc gagcgtcgag gtggtgccgt tcttcagcac
      601 cgacatcagc acggagcggt cgccgtccac gtgcacgatg ttggtctgcg gcgagttgcc
      661 gtcgtgcacg tgcgccacgt cgcggatgta gatcgtgctg ccgttgacga ccttgatcgg
      721 caggtcgccg atttcgtcga tcaggtccgg agcgttgttg agcaggatcg ggtactcgat
      781 cgaaccgatc ttctgcgtgc cgacgggcag gatcacgttc tgggcggcaa gcgcattggc
      841 gacgtccacc gccgacaggc cgcgcgactg cagtgccgcg agatcgaggt cgatctgcac
      901 ctgccgctgc ttgccgccgt acggccacgg cacggccgcc ccctgcaccg tgatcagccg
      961 cgagcggatc tggttcgtgc cgatgtcgaa caggttctgc tcggtcagtc ccttgcccga
     1021 cagcgcgagc tgcaggatcg gcacggtgga ggcgttgtag ttcaggatca ggggcggtgt
     1081 catgcccgcc ggcatctgcc gggtcatggt ctgggagatg gccgtcacct gggcgttggc
     1141 cgtcgcgatg tcgacgcccg ggtggaagaa gatcttcacg atgcccatgc ccgggtacgt
     1201 cgtcgcctcg atgtgctcca cgtcgttgac cgtcgtcgtg atcgcgcgct gcgacagcgt
     1261 ggtgacgcgg ccggccatgt cgtccacggg catgccggtg tactgccacg cgatggcgat
     1321 caccgggatg cggatctccg gaaagatgtc ggtcggggtc cgcatggccg cgagcggccc
     1381 gatgatgagc agcagcagcg ccatcaccac gaaggtgtag ggcttcctga gcgcgactcg
     1441 gacgacgttt acgagcaccg cttgcgcccc cggtctgcga cgcccggcag cagcccgtcg
     1501 aggttggcgt ggatgcgatt catcgccgcc atcagctgtg cttgctgccg cgtgctcagg
     1561 ccggcgagcg cctccgcgtg cgaaaggtcc cgcagcttcc agatcgtctc gagcaccggg
     1621 ccggccggct tgcgcaggta cagcgcgcga acccttcggt cgccacggac ggcacggcgt
     1681 tcgatcaggc cactcgcttc catgcgcgtg agcaggcgac cgagcgtcat cgggtccatg
     1741 tccgcgagat aggccaggcg cgcctgggag atgccctcgt gccgcgacag gtggccgagc
     1801 acgcggcact gctccagcgt caggccgtgg ccagcggcgt ggcgctcgaa attccgcgag
     1861 tagagtcgcg aaatgtcctt gagcaggaag ccgaagttcg cgagccggtc catcgttggt
     1921 gctggtgttg ttggcccggg aatcgggtgg gcggccgcac gagcggcgcc cgggccaatc
     1981 atatcaagtg atatgatc
//
LOCUS       DAOMFD010000311         1972 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_35620_length_1972_cov_35.012520, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000311 DAOMFD010000000
VERSION     DAOMFD010000311.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1972)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1972
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35620_length_1972_cov_35.012520"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1743)
                     /locus_tag="PL152_11345"
     CDS             complement(115..1743)
                     /locus_tag="PL152_11345"
                     /inference="COORDINATES: protein motif:HMM:NF009410.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HQR49924.1"
                     /translation="MKIKPFAITLDPGSSLANRTGSWRTVRPVYVDRLPPCNHACPAG
                     ENIQGWLFHAEAGQYEQAWRLLTQENPLPAVMGRVCYHPCETSCNRGHLDESVGINAI
                     ERFLGDEALRQGWKFEAPATASGKRVLVVGAGPSGLSAAYHLARLGHSVTIREAGPVA
                     GGMMRFGIPKYRLPREVLEAEIQRIVDLGVTIELNSKVSNVLEAMAGGRFDAVFLAVG
                     AHIAKRAYIPAGSAAKMLDAVSVLRGMEGADQPLLGRRVVVYGGGNTALDVARTAKRL
                     GATESIIVYRRTREKMPAHESELEEALQEGVLVKWLSTIKQAGESSITVEKMALDEHG
                     HAQPTGEFETLEADSVVLALGQDVDLSLLDGVPGLEIEDGVVKVDAGMMTGHPGIFAG
                     GDMVPGERTVTVGVGHGKLAARNIDAWLRGATWTHPPRHEIATFDRLNTWYYSDAPKS
                     TQPVLDTIRRQSTFEEVVGSFDEWTAQFEARRCLSCGNCFECDNCYGVCPDNAVVKLG
                     PGQRYAFNYDYCKGCGLCVSECPCGAITTVPEEI"
     gene            complement(1740..>1972)
                     /locus_tag="PL152_11350"
     CDS             complement(1740..>1972)
                     /locus_tag="PL152_11350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49925.1"
                     /translation="AKETGLFPVFEAEHGTVTHVSPIKKQLPVEEYLKPQQRFAHLFK
                     EPGRPDIVTRIQQIADRNIRRYRLLEGLERRA"
BASE COUNT          292 a          684 c          664 g          332 t
ORIGIN      
        1 ctcgaagtac gtcgcgatcg gcgagtccga ccgcatggtc ctgcgcttcg tcaaggagaa
       61 ataggggaca ttcccctatt tcaagttgaa ataggggaat gtcccctatt tctcctatat
      121 ctcctcgggg acggtggtga tcgcgccgca ggggcactcg ctcacgcaca gcccgcagcc
      181 cttgcagtag tcgtagttga acgcgtagcg ctggccgggc ccgagcttga cgaccgcgtt
      241 gtcggggcac acgccgtagc agttgtcgca ctcgaagcag ttgccgcacg acaggcagcg
      301 gcgcgcctcg aactgcgcgg tccactcgtc gaagctgccg acgacctcct cgaaggtcga
      361 ctgccggcgg atcgtgtcga gcacgggctg cgtggacttc ggcgcgtccg agtagtacca
      421 ggtgttgagc cggtcgaacg tcgcgatctc gtgccggggc ggatgcgtcc acgtcgcgcc
      481 ccgcagccac gcgtcgatgt tgcgcgccgc cagcttgccg tggccgacgc cgacggtgac
      541 ggtgcgctcg ccgggcacca tgtcgccgcc cgcgaagatg ccggggtgcc ccgtcatcat
      601 gcccgcgtcg accttgacga ccccgtcctc gatctcgagt cccggcacgc cgtcgagcag
      661 cgacaggtcc acgtcctggc cgagcgcgag caccaccgag tcggcttcga gggtctcgaa
      721 ctcgccggtc ggctgcgcgt ggccgtgctc gtcgagcgcc atcttctcga ccgtgatgga
      781 cgattcgccg gcctgcttga tcgtcgacag ccacttcacg agcacgcctt cctgcagcgc
      841 ctcctcgagt tccgactcgt gcgccggcat cttctcgcgc gtgcgccggt acacgatgat
      901 cgactccgtg gcgccgaggc gcttcgccgt gcgcgcgacg tcgagcgcgg tgttgccgcc
      961 gccgtagacg acgacgcgtc gcccgagcaa tggctggtct gcgccttcca tgccgcgcag
     1021 caccgacacc gcatcgagca tcttcgccgc gctgcccgcg ggaatgtacg cgcgcttggc
     1081 gatgtgcgcg cccacggcga ggaacaccgc atcgaaccgc ccgcccgcca tggcctcgag
     1141 cacgttcgac accttggagt tgagttcgat cgtgacgccg aggtccacga tgcgctggat
     1201 ctccgcctcc aggacctcgc gcggcagccg gtacttgggg atgccgaaac gcatcatgcc
     1261 gcccgcgacc ggtccggctt cgcggatcgt caccgagtgc ccgagccgcg caaggtgata
     1321 ggcggccgac aggccggacg gtcccgcgcc gacgacgagc acgcgcttgc cgctcgcggt
     1381 cgcaggcgcc tcgaacttcc aaccctgccg cagcgcctcg tcgccgagga agcgctcgat
     1441 cgcattgatg ccgaccgact cgtcgagatg cccgcggttg caggaggtct cgcacgggtg
     1501 gtagcagacc cgtcccatca cggcgggcag ggggttttcc tgcgtgagca ggcgccaggc
     1561 ctgctcgtac tggccggcct cggcgtggaa cagccagccc tggatgttct cgcccgccgg
     1621 gcacgcgtga ttgcagggcg gcaggcggtc cacgtacacg gggcggaccg tgcgccacga
     1681 gcccgtgcga ttcgcgaggc tggagccagg atcgagcgtg atcgcaaagg gcttgatctt
     1741 catgcgcggc gctccaggcc ttcgagcagc cggtagcggc ggatgttgcg gtccgcgatc
     1801 tgctggatgc gcgtcacgat gtcgggccgg cccggctcct tgaacaggtg cgcgaagcgc
     1861 tgctgcggct tgaggtattc ctcgaccggc aactgcttct tgatcggcga gacgtgagtg
     1921 acggtgccgt gctcggcctc gaacaccggg aacagccccg tctccttggc ga
//
LOCUS       DAOMFD010000312         1960 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_36018_length_1960_cov_36.313386, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000312 DAOMFD010000000
VERSION     DAOMFD010000312.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1960)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1960
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36018_length_1960_cov_36.313386"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..826)
                     /locus_tag="PL152_11355"
     CDS             complement(<1..826)
                     /locus_tag="PL152_11355"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HQR49926.1"
                     /translation="MLRWFERLVTPYPEHAPAAPPKGFFAFLWACTEGLRPYILAMTL
                     CTAVIGAFEALLFAMLGRIVDWLAKVEPSRLWIEERGTLLLLGSILAGSVVLVALQTI
                     LKHQTLAANFPMRLRWNFHRLLLGQSMSFYQDEFAGRVATKVMQTALAVRDTWFTVAD
                     ILVFTVIYFVTLVSVVGHFDLLMLAPFLGWAACYGIALAYFVPRLGKIGAAQADARSL
                     MTGRVTDAYTNIATVKLFSHTQREAGYARAAMQDFMQTAYAQMRLVSGFEIANHVLS"
     gene            1023..>1960
                     /locus_tag="PL152_11360"
     CDS             1023..>1960
                     /locus_tag="PL152_11360"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HQR49927.1"
                     /translation="MVDMDPAGLRKTLEQIDRAAQEHAEWHANVLRSIVCETPCDPND
                     LTASAHLLCRFGHWYYEQSAAEIREDPIFAAIGVEHRLVHRIAARILREVAAGRPVDR
                     EDFDDMLAGSARLRGDLDMLRRDVQGALRHRDERTGAFDRAQVLPELRHWRALVKQGV
                     QPCCIAFMDLDHLADINRKHGHPTGDAVLAAAVRYLRDHLRPASKVFRYGGDEFLISL
                     PGADFADAHELIKRVRQGLATDPLSGLPEHLEYPVTASFGLARLDPDARVEDSIERAA
                     QALLIAKSSGGDRAINWDESVITRRDLRRLEVDRHET"
BASE COUNT          335 a          660 c          678 g          287 t
ORIGIN      
        1 tgctgagcac gtggttggcg atctcgaagc cgctcacgag gcgcatctgc gcgtacgcgg
       61 tctgcatgaa gtcctgcatc gcggcgcgtg cgtagccggc ctcgcgctgg gtgtgcgaga
      121 agagcttgac ggtcgcgatg ttggtgtacg cgtccgtgac gcggccggtc atgagcgagc
      181 gcgcgtccgc ctgcgcggcg ccgatcttgc cgagcctggg cacgaaatac gcgagcgcga
      241 tgccgtagca cgcggcccag ccgaggaacg gcgcgagcat cagcaggtcg aagtggccga
      301 cgaccgagac cagcgtgacg aaatagatga ccgtgaacac gagaatgtcg gcaaccgtga
      361 accacgtgtc gcgcaccgcc agcgccgtct gcatgacctt ggtcgcgacg cgccccgcga
      421 actcgtcctg gtagaagctc atgctctggc cgagcagcag gcgatggaag ttccagcgca
      481 gccgcatcgg gaagttggcg gccagcgtct ggtgcttcag gatcgtctgc agcgcgacca
      541 gcacgacgct gccggcgagg atcgatccga gcagcagcag ggtgccgcgc tcctcgatcc
      601 agagccgcga cggctcgacc ttcgcgagcc agtccacgat gcgaccgagc atcgcgaaga
      661 gcagcgcctc gaacgcgccg atcaccgcgg tgcagagcgt catcgcgagg atgtagggac
      721 gcaggccctc ggtgcacgcc cacaggaatg cgaagaagcc cttcggtggc gcggcggggg
      781 cgtgctccgg gtaaggcgtg acgagtcgtt cgaaccagcg gagcacggca cgttctccga
      841 cgcgttgcgg agccgacatc ctacgcgggc gggcgtccgg gccctagagg gcgcgggtcc
      901 ggcggccaat tccggaatct gtgatccggt gcacggtaat agtgacctcc tggcgcgctc
      961 gaaagcttgt gccgccggtc caaacgggag gaacctgagc ccgtcacgac ggaggatcac
     1021 acatggtcga catggatcct gccggactac gcaagaccct cgagcagatc gatcgagccg
     1081 cgcaggagca tgccgaatgg catgccaacg tattgcggtc catcgtctgc gagactcctt
     1141 gcgatccgaa tgatctcacc gcgtccgcgc acctgctgtg tcgcttcggt cactggtact
     1201 acgagcaatc ggcggccgag atacgcgagg acccgatctt cgcggcgatc ggcgtcgagc
     1261 atcggctggt tcaccgcatt gccgcacgca tcctgcgcga ggtggcggcg ggccggcccg
     1321 tggaccgcga ggacttcgac gacatgctgg cgggcagcgc gcgcctgcgc ggcgacctcg
     1381 acatgctgcg gcgcgacgtc cagggggcgc tgcggcaccg cgacgagcgg acgggtgcct
     1441 tcgaccgcgc acaggtgctt cccgagcttc gccactggcg tgcactggtc aagcagggcg
     1501 tgcagccctg ctgcatcgcc ttcatggacc tcgaccatct cgccgacatc aaccgcaagc
     1561 acggccatcc gaccggggac gcggtcctcg ccgcggcagt gcgctacctc cgggatcacc
     1621 tccgcccggc cagcaaggtg ttccgctatg gcggggacga gttcctgatc tcgctgccgg
     1681 gcgccgattt cgcggacgcc cacgagctca tcaagcgggt ccgccagggg ctggccaccg
     1741 acccgctgtc cggcctgccg gagcacctcg agtacccggt cacggcgtcc ttcgggctcg
     1801 cgaggctcga cccggacgca cgcgtcgagg actcgatcga gcgcgcggcg caggccttgc
     1861 tgatcgccaa gagctccggc ggcgaccgcg ccatcaactg ggacgaatcg gtcatcacgc
     1921 ggcgcgacct ccgccggctg gaagtcgaca ggcacgagac
//
LOCUS       DAOMFD010000313         1949 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_36384_length_1949_cov_28.828933, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000313 DAOMFD010000000
VERSION     DAOMFD010000313.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1949
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36384_length_1949_cov_28.828933"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /locus_tag="PL152_11365"
     CDS             complement(<1..1026)
                     /locus_tag="PL152_11365"
                     /inference="COORDINATES: protein motif:HMM:TIGR03505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimV/HubP family polar landmark protein"
                     /protein_id="HQR49928.1"
                     /translation="MSPNALYALGLGEIHLHSALNQPFDADIDLVAAGDEDLSALRAS
                     LAPSDTFARYGLDKPGYLADFTFRVVRGPDGKDYLKVTSPRPVTEPFVTLLVQADWPR
                     GKLLREYTVLLDPPVFAPAQAAAEPQVATPRVQSGASAPAPAPRAESAPSSAAGAAGA
                     VTPTSAAAGSTYRVRPNDTLWKIASEAHPGSRSDVNRAMVAIYQANPHSFDGNINVLR
                     AGSSLRIPDADEISAIPAAAAAAEVSRQYQLWRESSAAAQPAESTAGRLKLVTPEQGT
                     TAASTATTPAPAGAPPAGAPTTDMQARVKQLEADLAEARRLLEVRNAELATLQGQAAA
                     GQPAAGQP"
     gene            complement(1269..>1949)
                     /gene="aroC"
                     /locus_tag="PL152_11370"
     CDS             complement(1269..>1949)
                     /gene="aroC"
                     /locus_tag="PL152_11370"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HQR49929.1"
                     /translation="IARKYLAEHAGVVVRGYLAQAGDVVIEPRDLAEADRNPFFCPDP
                     ARVPDLEALIERLRREGDSIGARVTVVATGVPPGLGEPVFDRLDADLAHAMMGINAVK
                     GVEVGDGFAVVGQRGSQHRDELTPGGFVSNHAGGTLGGISSGQPLVVSVALKPTSSIV
                     IPGRTIDVEGRPVEVSTTGRHDPCVGIRAVPIAEAMMALVLMDHLLRHRAQNAGVTTV
                     TPDICRGA"
BASE COUNT          289 a          685 c          689 g          286 t
ORIGIN      
        1 gggctggccc gcggcaggct gccctgcggc ggcctggccc tgcagcgtcg ccagctccgc
       61 gttgcggact tcgagcaggc ggcgcgcctc ggcgagatcg gcctcgagct gcttgacgcg
      121 cgcctgcatg tcggtcgtcg gcgcgccggc cggcggcgcc ccggcaggtg ccggagtcgt
      181 ggcggtcgat gctgcggtcg tgccctgctc gggcgtgacc agtttcagtc gtcctgcggt
      241 cgattcggca ggttgtgcgg ccgccgagct ctcgcgccac agctggtact gccgcgagac
      301 ctccgccgcc gctgcggctg cggggatcgc cgagatttca tcggcgtccg gaatcctcag
      361 cgagctgccg gcgcgcagga cattgatgtt cccgtcgaac gagtgcgggt tggcctggta
      421 gatcgccacc atcgcccggt tgacgtcgga ccgcgacccg gggtgagcct cgctcgcaat
      481 cttccataga gtgtcgttcg ggcggacacg gtaggtcgac ccagcggcgg cggatgtcgg
      541 agtgactgcg ccggctgcgc cggctgcgct ggacggcgca gactcagccc ggggcgccgg
      601 tgcaggggcg gaggcccccg actgcacgcg cggggtcgcg acctgcggct cggcagctgc
      661 ctgcgccggc gcaaagaccg gcgggtccag cagcacggtg tactcgcgca acagcttgcc
      721 gcgcggccag tcagcctgga cgagcagcgt gacgaacggc tcggtgacgg ggcgcggcga
      781 agtgaccttg aggtaatcct tgccatccgg gccgcggacg acccggaacg tgaaatccgc
      841 gaggtagccg ggcttgtcga ggccgtaacg cgcaaacgtg tcgctcggag ccagcgacgc
      901 ccgcagcgcc gacaggtcct cgtcgcccgc cgccacgaga tcgatatcgg catcgaaagg
      961 ctggttgagc gccgaatgga ggtgaatctc gccgaggccg agcgcgtaga gcgcgttcgg
     1021 cgacatcagg gcgctcagga gtaggacacg gcgaacggct gacttcatcg atgctcccac
     1081 cagtacgaca tgacccgggc ggcagacgcc atgtccaccg ggatcgggct cgttggctgc
     1141 tgcgaattaa acataactat aaccgacgct ctcacccgcg cccacagccc cgcggatccg
     1201 ccaaccttgt agcgcgtcac ggtcccgcga gatcggacgc gccgcacact tccgcgctct
     1261 tgccccgatc aggccccgcg gcagatgtcc ggcgtgaccg tagtgacccc cgcattctgg
     1321 gcgcgatgcc tgagcaggtg atccatcagc acgagcgcca tcatggcctc ggcgatcggc
     1381 acggcccgga tcccgacgca cgggtcgtgg cgtccggtgg tgctgacctc gacgggccgc
     1441 ccttcgacgt cgatggtgcg gcccgggatc acgatgctcg aggttggctt cagcgcgacg
     1501 ctcacgacca gcggctggcc ggaggagatg ccacccagcg tgcctccggc gtgattggac
     1561 acgaagccgc ccggggtcaa ctcgtcgcga tgctgcgacc cgcgctgccc gacgaccgca
     1621 aagccgtccc ccacctcgac tcccttgact gcgttgatgc ccatcatcgc atgcgcgagg
     1681 tccgcgtcca gcctgtcgaa caccggctcg ccgaggcccg ggggcacgcc ggtcgcgacg
     1741 accgtgaccc gcgctccgat ggaatcgccc tcgcgacgca ggcgctcgat cagcgcctcg
     1801 aggtccggca cgcgcgccgg gtccgggcag aagaacggat tacggtccgc ctccgcgagg
     1861 tcgcgcggct cgatgaccac gtcgccggcc tgcgcgagat agccgcgaac gacgacgccg
     1921 gcgtgctcgg cgaggtactt gcgcgcgat
//
LOCUS       DAOMFD010000314         1925 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_37217_length_1925_cov_29.291444, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000314 DAOMFD010000000
VERSION     DAOMFD010000314.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1925)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1925
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37217_length_1925_cov_29.291444"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="PL152_11375"
     CDS             complement(<1..328)
                     /locus_tag="PL152_11375"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR49930.1"
                     /translation="METSKPHIRSVGHGPTVVLLHSSGSSGRQWEGLVTALQSRYRLH
                     AIDLHGHGSTPAWTAARPMRLEDDLTLVEPLLSGSGRVHLVGHSYGGALALKLAARMP
                     ERIASVA"
     gene            complement(493..1479)
                     /locus_tag="PL152_11380"
     CDS             complement(493..1479)
                     /locus_tag="PL152_11380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49931.1"
                     /translation="MEAVADITIEAPAAETAAVSGDERSRYAKAIEVSKRVRWDIDRD
                     VIRGRRFDFGKKFLPDGLSKLDELAFVTAAERRFLSQIQGRTYANVFGLVERFIGAKV
                     LEISREHWLGDQTAMEALVRFTDEELKHQELFRRLEAMAAEGMPAGYHFVPQPNDVAA
                     VVLGKSTWAVLGLTLDIELFTQAHYRASIDPDPDLSPLWKDVFLFHWKEESQHAILDE
                     LEWKRENARITPAERDEAVNDLIALVAAVDGIVQAQAQSDTQYFVLQAGRSFTAQQRA
                     AIHAKLLEAYRWQYIFSGVQDARFLEVLGGMITPAQGERIHAALGSIASATN"
     gene            complement(1516..>1925)
                     /locus_tag="PL152_11385"
     CDS             complement(1516..>1925)
                     /locus_tag="PL152_11385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HQR49932.1"
                     /translation="ATDRDRAADLDGWARRAAAANGFGEARADAQVTQVSHDVHRLAI
                     EARGARSASLAAVLATLMATASAAAQRLRASWARARSARATYRALRELDARTLHDLGL
                     DRSEMRSVAAEIAGESDRTRAQALRTLRALSLF"
BASE COUNT          283 a          630 c          674 g          338 t
ORIGIN      
        1 ccgcgacact ggcgatgcgc tccggcatcc gcgccgccag cttcagcgcc agggcaccgc
       61 cgtaggagtg tccgacaagg tgcacgcgac cggagcccga gagcaacggc tccaccagcg
      121 tcaggtcgtc ttcgaggcgc atgggccgcg ccgcagtcca cgccggggtc gagccgtgcc
      181 cgtgcagatc gatcgcatgc aggcggtagc gcgactgcag tgccgtcacg agcccctccc
      241 attggcggcc ggagcttccg ctcgagtgca gcagtacgac tgtcggtccg tgtccgacgc
      301 tgcggatatg cggcttgctg gtttccatcg cttgattccc ttgtggaacg ccagcggcgc
      361 gaggatgggg gcgcgctggc tggcgcgcgg caccgatcgc tggcgctgtc gcaggcgtgg
      421 ttgttgactg gatttgaatg cggcgccccg atcgccgtcg tcgggcgacc gaggcgcctg
      481 acctggctcg gctcagttcg tcgcgctggc gatcgatccg agcgctgcgt gaatccgctc
      541 gccctgcgcc ggcgtgatca tcccgcccag cacctcgagg aatcgcgcat cctgcacgcc
      601 ggagaagatg tactgccagc gataggcctc aagcagcttg gcgtggatcg cggcgcgctg
      661 ctgtgcggtg aacgaacgcc cggcctggag cacgaagtac tgcgtgtcgg actgcgcctg
      721 ggcctggacg atcccgtcaa cggcggccac cagcgcgatc aggtcgttga ccgcctcgtc
      781 acgctccgcc ggtgtgatgc gcgcgttctc acgcttccac tcgagctcgt cgaggatcgc
      841 gtgctgcgac tcctccttcc agtggaacag gaacacgtcc ttccacagcg gcgagaggtc
      901 gggatcgggg tcgatgctgg cgcgatagtg cgcctgggta aagagctcga tgtcgagcgt
      961 caggccgagg accgcccatg tcgacttgcc cagtaccacg gctgcgacat cgttcggttg
     1021 cggcacgaag tgatacccgg cgggcatgcc ctcggcggcc atcgcttcga gccgccggaa
     1081 cagctcctgg tgcttgagct cttcgtccgt gaagcggacc agggcttcca ttgccgtctg
     1141 gtcgccgagc cagtgctcgc ggctgatttc gaggaccttc gcgccgatga agcgctcgac
     1201 gagtccgaag acattggcat aggttcgccc ctggatctgg ctgagaaagc ggcgctcggc
     1261 ggcagtgacg aaggcgagct cgtcgagctt cgacagtccg tcgggcagaa acttcttgcc
     1321 gaagtcgaag cgtcgtccgc ggatgacatc gcggtcgatg tcccagcgga cgcgcttgga
     1381 aacctcgatg gccttcgcgt agcggcttcg ttcgtcgccg ctgacggccg cggtttcggc
     1441 tgcgggggct tcaatggtga tgtctgcaac agcttccatg ggattgctct cctgacggtc
     1501 ggggttgaac gacacttaaa aaagggaaag ggcgcggagc gtgcgcaggg cctgcgcacg
     1561 ggtacgatcg gactcgccgg cgatttcggc ggcgaccgag cgcatctcgc tgcggtcgag
     1621 gccgaggtca tgcagcgtgc gggcgtccag ctcgcggagc gccctgtagg tggcgcgtgc
     1681 ggacctcgcc cgcgcccagg aggcgcgcag tcgttgcgcg gccgccgagg cagtggccat
     1741 cagcgttgca aggaccgcgg ccagactggc gctgcgtgcg ccgcgggcct cgatcgccaa
     1801 tcgatggacg tcgtgactta cttgcgtgac ttgtgcgtct gccctcgctt ccccgaaacc
     1861 gttcgcggca gccgccctcc gcgcccagcc gtccaggtcg gcggcgcggt cgcggtcggt
     1921 tgcgt
//
LOCUS       DAOMFD010000315         1880 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_38836_length_1880_cov_28.535890, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000315 DAOMFD010000000
VERSION     DAOMFD010000315.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1880)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1880
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38836_length_1880_cov_28.535890"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(151..897)
                     /locus_tag="PL152_11390"
     CDS             complement(151..897)
                     /locus_tag="PL152_11390"
                     /inference="COORDINATES: protein motif:HMM:NF023197.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 family protein"
                     /protein_id="HQR49933.1"
                     /translation="MRTTRSCWSLAAPVAAMLAFALVTSSVARADGQFPTVSPDGLQL
                     VKSTDHGAVYVKQGATFSQYRQIGFLECYVQFAKDWAQDYNENEADLSMQVTPEDQTR
                     IKNWLSAEFNKVFVDELGKNGDFQIVTSQAAGVLMLRPALLNVVVTAPTTDSPSPSVD
                     LVASAGQMTLMLELWDGGTSTLLARVIDGEADTSFGGMAHQANQVTNEAAANTILKSW
                     ADRLRKALDAARAAAPSPAPPAAPAPAVVN"
     gene            complement(936..>1880)
                     /locus_tag="PL152_11395"
     CDS             complement(936..>1880)
                     /locus_tag="PL152_11395"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HQR49934.1"
                     /translation="VSQQTDKPRVRSIMLGVRSKVMEGHTLADGLAEFPRSFPEIYRA
                     TVAASEQSGHLDAVLERLAEYTDNRQQLRERTLSAMLYPVLLFLVCVVIVFFLLVSVV
                     PKVVEVFRTSQAQLPLLTQGLIAFSDFMRHFGIWLVVGVAGSIFAFNRWVQLEANRRV
                     WHQFLLSLPLIGKVVRGSNTARFARTLSTLTASTVPVLEALRISSEVVSNLPMKAAVE
                     AAAVRVREGAPIGKSLAVGRLFPPMMIHLISSGESSGELDTMLERAAIHQERELDALL
                     QGVVGLLGPLMILLMGGLVLVIVLAMLLPIFELNQLVK"
BASE COUNT          303 a          632 c          645 g          300 t
ORIGIN      
        1 gctccggccg ggcggccgcc tgttccatta cacgggggcg ccgaattcgc gttcgagggg
       61 ccgcaacctc gccggcgagg tggtcgagcg cttgcgtgtc gccgggttcg acgcgcgacc
      121 cgaactcgac ggggtgctcg cgacccggcg ctaattgacg acggcaggcg cgggcgcggc
      181 cgggggtgcc ggcgagggcg cggcggcgcg tgccgcgtcc agcgccttgc gcagccggtc
      241 ggcccagctc ttcaggatcg tattcgcggc cgcctcgttg gtgacctggt tggcctggtg
      301 cgccatgccg ccaaagctcg tatcggcctc gccatcgatg acccgcgcga gcagcgtgct
      361 cgtgcctccg tcccagagct ccagcatgag cgtcatctgg cctgcggatg caaccaggtc
      421 cacgctcggt gagggcgaat ccgtggtggg cgccgtgacc accacgttca ggagcgcggg
      481 acgcagcatc agcaccccgg ccgcctgcga cgtgacgatc tggaagtcgc cgttcttgcc
      541 cagttcgtcg acgaacacct tgttgaactc ggcggacagc cagttcttga tgcgcgtctg
      601 gtcctcgggc gtgacctgca tggacaagtc ggcctcgttc tcgttgtagt cctgggccca
      661 gtccttggcg aactgcacgt agcactcgag gaacccgatc tggcggtatt gcgagaaggt
      721 cgccccctgc ttgacgtaca ccgctccgtg gtcggtcgac ttgacgagtt gcagcccgtc
      781 cgggctgacc gtcgggaatt gtccgtcggc gcgcgcaacg ctcgacgtca ccagcgcaaa
      841 tgcgagcatg gcggcgacgg gggcggcgag tgaccagcag ctcctggttg ttctcatggt
      901 gggctccgtg ccggcgcagg ctctgggcga aacgtctatt tcaccagctg gttgagctcg
      961 aagatcggca gcagcatcgc cagcacgatc acgagcacca ggccgcccat cagcaggatc
     1021 atgagcgggc cgagcaggcc gaccacgccc tgcagcagcg cgtcgagctc gcgctcctgg
     1081 tggatggccg cgcgctcgag catcgtgtcg agttcgccgc tcgattcgcc gctcgagatc
     1141 aggtggatca tcatcggcgg gaacaagcgc ccgacggcca gggacttgcc gatcggcgcg
     1201 ccctcgcgga cccgcacggc ggctgcctcg accgccgctt tcatcggcag gttcgacacg
     1261 acctcggacg agatgcgcag cgcttccagg accgggacgg tactcgccgt gagcgtgctc
     1321 aacgtgcgtg cgaagcgcgc ggtattgctg ccgcgcacca ccttgccgat cagcggcaac
     1381 gacagcagga actggtgcca gacccggcga ttggcctcga gctgcaccca gcgattgaac
     1441 gcgaagatgc tgcccgccac cccgaccacc agccagatgc cgaagtgccg catgaagtcg
     1501 ctgaacgcga tcagtccctg cgtgagcaga ggcagctgcg cctggctggt gcggaacacc
     1561 tcgaccacct tggggacgac cgacacgagc aggaagaaca cgatgacgac gcacaccagg
     1621 aacagcagca ccgggtagag catcgcgctc agcgtgcgct cacgcagttg ctgccggttg
     1681 tcggtgtatt ccgcgagccg ctcgagcacc gcgtcgaggt ggccggactg ctcgctggcc
     1741 gcgaccgtgg cgcgatagat ctcgggaaac gaccgcggga actcggcgag cccgtcggcg
     1801 agcgtgtggc cctccatcac cttggagcgg acgccgagca tgatgctgcg cacgcgcggc
     1861 ttgtcggtct gctgcgagac
//
LOCUS       DAOMFD010000316         1876 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_38999_length_1876_cov_37.386601, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000316 DAOMFD010000000
VERSION     DAOMFD010000316.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1876)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1876
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38999_length_1876_cov_37.386601"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1876
                     /gene="aceE"
                     /locus_tag="PL152_11400"
     CDS             <1..>1876
                     /gene="aceE"
                     /locus_tag="PL152_11400"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045250 - cytosolic pyruvate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004738 - pyruvate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006086 - acetyl-CoA biosynthetic process
                     from pyruvate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HQR49935.1"
                     /translation="HPWLMPDFWQFPTVSMGLGPMMAIYQARFTRYLEHRGIVPATDS
                     KVWAFLGDGEMDEPESLGALTMAVREKLDNLVFVINCNLQRLDGPVRGNGKIIQELEA
                     AFLGAGWNVIKVIWGSRWDPLLAQDTRGLLRRLMMECVDGEYQNFKAKGGAYTREHFF
                     GKYAELKEMVANMSDDDIWRLNRGGHDPHKVYNAYAAAVAHQGQPTVILAKTIKGYGM
                     GRAGEGMNITHQQKKLDDEALKDFRDRFNIPVSDEDIAKVPYYRPAEGSEELEYLRER
                     RRSLGGFLPQRRTRAAALTVPPLETFAPLLEASGDREISTTMALVRILSILVKDKGIG
                     AHVVPIVPDEARTFGMEGMFRQIGIYSSMGQLYTPQDADQLLYYREDRKGQILEEGIN
                     EAGAMCSWIAAGTSYANHGIAMVPFYIYYSMFGFQRVGDFAWAAGDMQSRGFLIGGTA
                     GRTTLAGEGLQHQDGHSQLVATTIPNCVAYDPAYAYELAVIVQDGLRRMYAEQENVFY
                     YITCMNENYAQPALPSGVEEGILKGAYLLKSGGRGKVRVNLLGSGTILREVLAAAEIL
                     EGEFGVPADVFSVTSFSELRREALDAERWNLLHPDQPPRQPYLRRCLEGHAGPFVAAT
                     "
BASE COUNT          335 a          628 c          632 g          281 t
ORIGIN      
        1 cgcatccgtg gctgatgccg gacttctggc agttcccgac ggtgtcgatg ggcctcggcc
       61 cgatgatggc gatctaccag gcgcggttca cgcgctacct cgagcatcgc ggcatcgtgc
      121 cggcgaccga cagcaaggtg tgggcgttcc tcggcgacgg cgagatggac gagcccgagt
      181 cgctcggcgc gctcaccatg gccgtgcgcg agaagctcga caacctggtg ttcgtcatca
      241 actgcaacct gcagcgcctg gacggcccgg tgcgcggcaa cggcaagatc atccaggaac
      301 tggaagcagc cttcctgggg gctggctgga acgtcatcaa ggtgatctgg ggctcgcgct
      361 gggacccgct gctcgcgcag gacacgcgcg ggctgctgcg ccggctgatg atggagtgcg
      421 tggacggcga gtaccagaac ttcaaggcca agggcggcgc ctacacgcgc gagcatttct
      481 tcggcaagta cgccgagctg aaggagatgg tcgccaacat gtcggacgac gacatctggc
      541 gcctcaatcg cggcggccac gacccgcaca aggtctacaa cgcctatgcg gccgcggtcg
      601 cgcaccaggg acagccgacg gtgatcctcg ccaagaccat caagggctat ggcatgggtc
      661 gcgccgggga aggcatgaac atcacccacc agcagaagaa gctggacgac gaggcgctca
      721 aggattttcg cgaccggttc aacatcccgg tgtccgacga ggacatcgcc aaggtgccgt
      781 attaccgccc cgccgagggc agcgaggagc tcgagtacct gcgcgagcgc cgccgctcgc
      841 tgggaggatt cctcccgcag cgacgcaccc gcgcggccgc gctcacggtc ccgccgctcg
      901 aaacgtttgc cccgctgctc gaggccagcg gcgaccgcga gatctccacg accatggcgc
      961 tggtgcgcat cctctcgatc ctggtcaagg acaaggggat cggcgcacac gtcgtgccga
     1021 tcgtgccaga cgaagcgcgg acgttcggca tggaagggat gttccggcag atcggcatct
     1081 actcctcgat gggtcagctc tacacgccgc aggacgcgga ccagctgctc tattaccgcg
     1141 aggacaggaa ggggcagatc ctcgaggaag gcatcaacga agccggcgcg atgtgcagct
     1201 ggatcgccgc cggcacgtcc tacgccaacc acggcatcgc gatggtgccg ttctacatct
     1261 actactcgat gttcggcttc cagcgggtcg gcgatttcgc ctgggccgcc ggcgacatgc
     1321 agtcgcgcgg attcctgatc ggcggcacgg cgggacgcac cacgctcgcg ggcgagggcc
     1381 tgcagcacca ggacggccac agccagctcg tcgcgacgac catcccgaac tgcgtcgcgt
     1441 acgacccggc ctacgcgtac gagctcgccg tcatcgttca ggacgggctg cgacggatgt
     1501 acgccgagca ggagaacgtc ttctactaca tcacctgcat gaacgagaat tacgcgcagc
     1561 cggcgcttcc gtccggggtc gaggagggca tcctgaaggg tgcgtacctg ctcaagtccg
     1621 gcggccgcgg caaggtgcgc gtcaacctgc tcgggtccgg gaccatcctg cgcgaggtgc
     1681 tggccgccgc ggagatcctg gaaggcgagt tcggcgtgcc ggccgacgtg ttcagcgtca
     1741 cgagcttctc cgagttgcgc cgcgaagcgc tcgacgccga gcgctggaac ctgctgcacc
     1801 ccgaccagcc gccgcgtcaa ccctacctgc ggcgatgcct cgagggccac gccgggccgt
     1861 tcgtcgcggc cacgga
//
LOCUS       DAOMFD010000317         1866 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_39420_length_1866_cov_35.414688, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000317 DAOMFD010000000
VERSION     DAOMFD010000317.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1866)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1866
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39420_length_1866_cov_35.414688"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1008
                     /locus_tag="PL152_11405"
     CDS             <1..1008
                     /locus_tag="PL152_11405"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HQR49936.1"
                     /translation="IDGSPQGFTAWRDRPYVAPVGDYPKGYAGYAAATNEQVLDAVNW
                     AYANDMQLLTHANGEAASDLLIATTTIAQLKNGGVKDRRPVLIHGQFLREDQVDSYKR
                     LGVLPSLFPMHTFYWGDWHTSKTVGPVLGQNISPTGWVRKRGMIFTSHHDAPVAFPDS
                     MRVLDATVTRVARGSGKIIGPDQRVDVITGLKAMTIWPAWQHYEEKSKGSIEVGKLAD
                     FVILSRDPTRGDVNTIDQIKVTETIKEGTTVFKLTAEEQRKADLMLRPGRDGANVFTR
                     FLTAAAVYRDVSQLPPYLRQPGTVEYFLNSPHDTACVVRVLDEMLLAMLQDGVVPAQA
                     D"
     gene            complement(1105..1407)
                     /locus_tag="PL152_11410"
     CDS             complement(1105..1407)
                     /locus_tag="PL152_11410"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HQR49937.1"
                     /translation="MRVGTGATARSGQDVVVHYTGWLTDGRKFDSSRDRGEPFSFGLG
                     AGQVIAGWDEGVAGMQVGGLRKLTIPPRLGYGEFGAGGVIPPNATLVFEVELLEVG"
     gene            complement(1446..1724)
                     /locus_tag="PL152_11415"
     CDS             complement(1446..1724)
                     /locus_tag="PL152_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49938.1"
                     /translation="MTMKKTELEKRKGLKIANALRQSGSRFDTTTPTDRRAQRERDRA
                     AGLVPFAVKLPGELLRQLHEQSQQRQLPLNELVEQLLRSGLKTKEEGR"
BASE COUNT          310 a          636 c          605 g          315 t
ORIGIN      
        1 atcgacggct cgccgcaggg cttcaccgcc tggcgcgacc ggccctacgt ggcgccggtg
       61 ggcgactacc cgaagggcta cgccggttac gccgcggcga ccaacgagca ggtgctggac
      121 gccgtgaact gggcctacgc caacgacatg cagttgctga cccacgccaa cggcgaggcg
      181 gcctcggacc tgctcattgc aacgacgacg atcgcgcagc tcaagaacgg tggcgtcaag
      241 gaccgccgtc cggtgctgat ccatggccag ttcctgcgcg aggaccaggt cgattcttac
      301 aagcggctcg gcgtgctgcc atcgctgttc ccgatgcaca ccttctactg gggcgactgg
      361 cacaccagca agaccgtggg cccggtgctc gggcagaaca tctcgccgac cggctgggtc
      421 cgcaagcgcg gcatgatttt cacgtcgcac catgacgcgc cggtggcgtt cccggactcg
      481 atgcgtgtgc tggatgccac cgtgacccgc gtggcacgcg gctctggcaa gatcatcggc
      541 cccgaccaga gggtcgacgt catcaccggg ctcaaggcga tgacgatctg gccggcgtgg
      601 cagcactacg aggagaagtc caagggctct atcgaggtgg gcaagctcgc cgacttcgtg
      661 atcctttcgc gtgacccgac caggggcgac gtcaacacga tcgaccagat caaggtgacc
      721 gagacgatca aggaaggcac caccgtcttc aagctgacag ccgaggagca gcgcaaggcg
      781 gacctgatgc tacgtccagg gcgggacggc gccaatgtgt tcacgcgctt cctcacggcg
      841 gccgcggtct accgcgacgt gtcgcagttg ccgccctacc tgcggcagcc cggcacggtc
      901 gagtactttc tcaacagtcc gcatgacacg gcgtgtgtcg tccgcgtact ggacgagatg
      961 ctgcttgcga tgctgcagga cggagttgtg ccggcgcaag cggactgagg gcgattgcag
     1021 gcaccggtac tccggtgccg ccgcggtgaa acttcttcca ggtgcgtgtt gcgccgtcgg
     1081 caccgcagac gcgagctggg cggctcagcc gacttcgagc agttcgacct cgaataccag
     1141 cgtcgcattc ggcggaatga caccgccggc gccgaactcg ccgtagccca gccgcggcgg
     1201 gatcgtcagc ttgcgcaggc cgccgacctg catgccggca acgccttcgt cccatccggc
     1261 gatgacctgc ccggcaccga ggccaaagga gaacggctcg ccacgatcgc ggctggaatc
     1321 gaacttgcgg ccatccgtga gccagccggt gtagtggacg acgacgtcct gcccggaacg
     1381 cgccgtggcg ccggtgccga cgcgcatttc ctcgtactgc aagccgctcg ctgtcgtgac
     1441 tgtgctcatc gtccttcctc cttcgtcttc aatccgctgc gcagcaactg ctcgaccagt
     1501 tcgttcagtg gcagctggcg ctgttgtgac tgctcgtgca gctgccgcag cagctcgccc
     1561 ggcagcttga ccgcgaaagg taccagcccg gcggcccggt cgcgctcgcg ctgcgcgcgc
     1621 cggtccgtcg gcgtcgtcgt gtcgaaccgc gaaccggact gccgcagggc gttggcgatc
     1681 ttcagtccct tgcgcttctc gagctccgtc ttcttcatcg tcatggcact tgaccccgcg
     1741 catgatcctg cgcataagcg cgcacgatcg actcgatgtc gctatctgcg gtgaacccga
     1801 gcgtgagcgc acgtgccgcg tcgaagcgcg cgggccagcc gtagacgatc ttcgcaatgc
     1861 gctcgt
//
LOCUS       DAOMFD010000318         1856 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_39782_length_1856_cov_32.856191, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000318 DAOMFD010000000
VERSION     DAOMFD010000318.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1856)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1856
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39782_length_1856_cov_32.856191"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="PL152_11420"
     CDS             <1..507
                     /locus_tag="PL152_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HQR49939.1"
                     /translation="DRVETTALGLLRQVREPWALEEPLAELVLRWAARLHEIGLDIAH
                     SHYHRHGAYLLEHADMPGFPREEQRLLSVVVGSHRRKLALEPCESLVPPWHVKSHFLI
                     VLLRLAVLLNRGRGPRALPRVDLAVRERSLELGFPKGWLAEHPLTEADLEQEAEYLRA
                     AGFRLRVA"
     gene            complement(517..>1856)
                     /gene="ppk1"
                     /locus_tag="PL152_11425"
     CDS             complement(517..>1856)
                     /gene="ppk1"
                     /locus_tag="PL152_11425"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020650005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HQR49940.1"
                     /translation="DEETEDLLRAVEGELASRRYGDAVRLETSVDCPAHLRDFLLEHF
                     ELGPEDHYLVDGPVNLHRLMSVCELVDRRDLKYPPFVPAVPRRLAGRHDLFEAIREKD
                     VLLHHPFHSFAPVIDFLRQAASDPHVLAIKQTLYRTGPESPVVDALVAAARAGKEVTV
                     VVELLARFEEAANIALANRLQEAGAHVMYGVVGYKTHAKLIMVVRRDPDRLRRYCHLG
                     TGNYHPQTTRQYTDYGLFTCDEAIGEDLHELFLQLTSPTRIARMRRILQSPFTLHEAM
                     LEKTRREIDHARAGRPARIIAKMNSLIEPQIVRALYEASIAGVRVDLIVRGICSLRPG
                     VPGISDNITVRSIVGRFLEHTRVYHFENGGEPETLCASADWMERNFFRRIEVCFPVLR
                     ASHRARVLADLELYLADNCDAWELAADGTYRRCSPGTSAAVAAQLALLETLAD"
BASE COUNT          263 a          636 c          674 g          283 t
ORIGIN      
        1 gaccgcgtcg agacgaccgc gctcggcctg ctccggcagg tgcgggaacc gtgggccctc
       61 gaggagccgc tcgcggagct cgtgctgcgc tgggccgcgc ggctgcacga gatcggactc
      121 gacatcgcgc actcgcacta ccaccggcac ggggcttacc tgctcgagca cgccgacatg
      181 cccggcttcc cgcgcgagga acagcggctg ctgtcggtcg tggtcggcag ccaccgtcgc
      241 aagctggcgc tcgagccgtg cgaatcgctc gtgccgccat ggcacgtcaa gtcgcatttc
      301 ctcatcgtgc tgctgcgcct cgcggtgctg ctgaaccgcg ggcgcggccc gcgggcgctg
      361 ccacgggtgg acctcgccgt gcgtgagcgc tcgctggaac tcggcttccc gaagggctgg
      421 cttgcggagc acccgctgac cgaggcggac ctcgagcagg aggcggaata cctgcgggcg
      481 gccgggttcc ggctgcgcgt ggcctgagcc ggcggctcag tcggccagcg tctcgagcag
      541 ggcgagctgg gcggccaccg ctgcggaggt tcccggcgag cagcggcggt aggtgccgtc
      601 ggcagcgagt tcccacgcgt cgcaattgtc ggccaggtag agttcgaggt ccgcgagcac
      661 ccgggcgcga tggctcgcgc gcagcaccgg gaagcacacc tcgatgcgac ggaagaagtt
      721 gcgctccatc cagtccgcac tcgcgcagag ggtctccggc tccccgccgt tctcgaagtg
      781 gtacacgcgc gtgtgctcga ggaatcgccc cacgatggaa cgcaccgtga tgttgtccga
      841 gatgcccggc acgcccggcc tgagcgagca gatgccgcgc acgatcaggt ccacgcgcac
      901 cccggcgatc gacgcctcgt agagcgcgcg cacgatctgc ggctcgatca gcgagttcat
      961 cttggcgatg atccgcgccg ggcggccggc gcgcgcgtgg tcgatctcgc gccgggtctt
     1021 ctcgagcatc gcctcgtgca gcgtgaacgg cgactgcagg atccggcgca tgcgcgcgat
     1081 gcgcgtcggg ctggtgagct gcaggaacag ctcgtgcaga tcctcgccga tcgcctcgtc
     1141 gcaggtgaac aacccgtagt cggtgtattg ccgcgtcgtc tgcgggtggt agttgccggt
     1201 gccgaggtgg cagtagcggc gcagccggtc cgggtcgcgg cgcaccacca tgatcagctt
     1261 ggcgtgcgtc ttgtagccga ccacgccgta catgacgtgc gcgccggcct cctggaggcg
     1321 attggcgagc gcgatgttgg ccgcttcctc gaagcgcgcc agcagctcga cgaccaccgt
     1381 gacttccttg ccggcgcgcg ccgcggccac cagcgcgtcg acgaccggcg actcggggcc
     1441 ggtgcgatac agcgtctgct tgatcgcgag cacgtgcggg tcggacgccg cctggcgcag
     1501 gaagtcgatg accggcgcga aactgtggaa cgggtggtgc agcaggacgt ccttctcgcg
     1561 gatcgcctcg aacaggtcgt ggcgacccgc gagccgcctc ggtaccgcgg gcacgaacgg
     1621 cgggtacttg aggtccctgc ggtcgacgag ctcgcacacg gacatcaggc ggtgcaggtt
     1681 gaccggcccg tccacgagat agtggtcctc gggccccagc tcgaagtgct cgagcaggaa
     1741 gtcgcgcagg tgcgccgggc agtcgaccga tgtctcgagc cgcacggcgt cgccgtagcg
     1801 ccgcgaggcg agttcgccct cgaccgcgcg cagcaggtct tccgtctcct cgtcgt
//
LOCUS       DAOMFD010000319         1844 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_40226_length_1844_cov_30.376747, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000319 DAOMFD010000000
VERSION     DAOMFD010000319.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1844
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40226_length_1844_cov_30.376747"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1599
                     /locus_tag="PL152_11430"
     CDS             <1..1599
                     /locus_tag="PL152_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007187138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HQR49941.1"
                     /translation="RATMAAYRPFLELALRSPWAVTILATLVVAATLWPASRLGTEFM
                     PELDEGDFLYMPTTLPGLSTDAARALLQQTDRLIRTVPEVERVFGKAGRAESATDPAP
                     IEMFETVVKLKPRSEWRPGMTPEKLKAELDARVKFPGLSNAWGYPIKTRIDMLATGIR
                     TPVGIKIMGPDLATIQRLGEQIENILRNVPGTTSVYAERTATGRYLDVDIDRVAAGRY
                     GLNVKDVQDIVGSAVGGMTVSSTVEGLERYPINLRYPQDFRDSLEKLRALPIVAPTGA
                     NVTLADVARVDVAEGPAMIRSENARPSGWVFVDIAGRDLGGYVKEAKARIASDLKLPP
                     GYSLGWSGQYEYLERAVERLKVVVPLTLAIIVLLLYLNFRNARDVLLILFALPFALVG
                     GIWLLYALGYDLSIAAAVGFIALAGVAAETGVVMVMYLDHAWEARCERARAEGRAPTR
                     AELHSAIVEGALLRLRPKLMTVITIIVGLLPIMWGSGTGSEVMRRIAAPMVGGMVSAT
                     VLTLVIIPSLYLIVNGWRLRGAVD"
     gene            1604..>1844
                     /locus_tag="PL152_11435"
     CDS             1604..>1844
                     /locus_tag="PL152_11435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49942.1"
                     /translation="MHDPTQHDAQRGGPSAGSCCHAEPAAAPSPAIHEHQHAGAAPAG
                     AASSSAHACCHAPAAPEVAKPATHDCCHGAPVAGVP"
BASE COUNT          274 a          632 c          654 g          284 t
ORIGIN      
        1 cgcgcgacga tggccgcgta ccggccgttc ctggagctcg cgctgcgctc gccgtgggcc
       61 gtcacgatct tggcgacgct ggtcgtggcg gcgacgctgt ggcctgcctc gcggctcggc
      121 acggagttca tgccggaact cgacgagggt gatttcctgt acatgcccac gacactccct
      181 gggctctcga ccgatgcggc gcgcgcgctg ctccagcaga cggaccgcct gatccgcacc
      241 gtgcccgagg tcgagcgcgt cttcggcaag gcaggacgcg ccgagtcggc gaccgatccg
      301 gcgccgatcg agatgttcga gaccgtcgtg aagctgaagc cccggagcga gtggcgaccg
      361 ggcatgacgc cggagaagct caaggcggag ctcgacgcgc gggtcaagtt ccccgggctg
      421 tcgaacgcct ggggctaccc catcaagacg cgcatcgaca tgctggcgac cgggatccgc
      481 acgccggtgg gcatcaagat catggggccg gacctcgcga cgatccagcg gctcggcgag
      541 cagatcgaga acatcctgcg caacgtgccg gggacgacct cggtctatgc cgagcggacc
      601 gcgaccgggc gatacctcga cgtggacatc gaccgcgtcg ccgccggccg ctacggcctc
      661 aacgtcaagg acgtgcagga catcgtaggc agcgcggtcg ggggcatgac cgtgagctcg
      721 acggtggagg gactggagcg ctacccgatc aacctgcgct acccgcagga tttccgcgac
      781 tccctcgaga agctgcgcgc gctgcccatc gtcgcgccca ccggtgccaa cgtcacgctc
      841 gccgacgtgg cccgggtcga tgtggcggaa ggcccggcga tgatccgcag cgagaacgcg
      901 cgcccctcgg gatgggtctt cgtggacatc gccggccgcg atctcggcgg ttacgtcaag
      961 gaggccaagg cgcggatcgc gtcggacctg aagctgccgc cgggctacag cctcgggtgg
     1021 tccgggcagt acgagtacct cgagcgtgcg gtggaacgac tgaaggtcgt cgtacccctc
     1081 acactcgcga tcatcgtgct gctgctctac ctgaatttcc gcaacgcgcg cgacgtgctg
     1141 ctgatcctgt tcgcgctgcc gtttgcgctg gtgggcggca tctggctcct gtacgcactg
     1201 ggctatgacc tgtcgatcgc cgccgcggtc gggttcattg cgctcgcggg cgtggccgcg
     1261 gagaccggcg tcgtcatggt catgtacctc gaccacgcct gggaggctcg ctgcgaacgg
     1321 gcaagggcgg agggcagggc gccgacccgc gccgaacttc acagcgcgat cgtcgaggga
     1381 gcgttgcttc gtctgcgtcc gaagctgatg accgtcatca cgatcatcgt gggcctgctg
     1441 cccatcatgt ggggttccgg cacggggtcc gaggtgatgc gccgcatcgc cgcgccgatg
     1501 gtgggcggca tggtctcggc gaccgtgctg acgctggtga tcatcccgtc gctgtacctg
     1561 atcgtgaacg gctggaggct gcgcggcgca gtagactagt cacatgcacg acccgaccca
     1621 gcacgatgcg cagcgtggag gcccgtccgc gggatcctgc tgccacgccg agccggcggc
     1681 ggccccgtcg ccggcgatcc atgagcacca gcatgccgga gcggcgcccg cgggcgccgc
     1741 gtcgtcttcg gcacacgcgt gctgccacgc gccggccgcg cccgaggtcg ccaagcctgc
     1801 gacgcacgac tgctgccacg gcgctccggt cgcgggtgtc ccgc
//
LOCUS       DAOMFD010000320         1838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_40466_length_1838_cov_36.254627, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000320 DAOMFD010000000
VERSION     DAOMFD010000320.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40466_length_1838_cov_36.254627"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /gene="sucC"
                     /locus_tag="PL152_11440"
     CDS             complement(<1..1000)
                     /gene="sucC"
                     /locus_tag="PL152_11440"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HQR49943.1"
                     /translation="MNLHEYQSKQLFERYGIPVPTGYVATTPDGAADAARRLGGSVWV
                     VKAQVHAGGRGKAGGVKVVRTVDDVAAAAAGMLGKRLTTKQTGADGLPINQVYVESGS
                     KIERELYLSLLLNRDTGRIAFVASAAGGMDIEEVAAKTPEKIIRVEVHPATGLQGSHC
                     RALAFGLGLKGAQVAEFETIARGLYKLYLECDASLVEVNPLIVTGEGRVVALDAKINI
                     EDNALFRQKDLVAWRDTSQEDEMERVAGEADLNYVSLDGDIACMVNGAGLAMATMDLI
                     KLHGGEPANFLDVGGGATRERVTTAFKLILENPKVKAILVNIFGGIVRCDMIAEGVI"
     gene            1188..>1838
                     /locus_tag="PL152_11445"
     CDS             1188..>1838
                     /locus_tag="PL152_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49944.1"
                     /translation="MPDPRPARAGPADLPWRVLGLLNIFRLTVPMVLLLVFFFDAPQR
                     SVGAHSPGLFIGICAAYFAFGLVSIQPVQRRWPSVEWLALFQLAVDTAAILMFVHASG
                     GAASGLGMLLIVPAGGTATIVSPRLALFTASMLALALLIQTVVAGLAGTSHSTDFLIA
                     GLTGASLFAVTLLAIPLANRLRESEALVQQRDIDLANLSELNEFIVQHLRESILVVD"
BASE COUNT          260 a          642 c          598 g          338 t
ORIGIN      
        1 cgatgacgcc ctcggcgatc atgtcgcagc ggacgatgcc gccgaagatg ttgaccagga
       61 tcgccttcac cttcgggttc tcgaggatca gcttgaacgc ggtggtgacg cgctctcgcg
      121 tcgcgccacc gccgacgtcg aggaagttgg caggctcgcc gccgtgcagc ttgatcaggt
      181 ccatggtcgc catggcgagg cccgcgccgt tcaccatgca ggcgatgtcg ccgtcgagcg
      241 agacgtagtt gaggtccgcc tcgccggcga cgcgctccat ctcgtcttcc tgcgacgtgt
      301 cgcgccaggc gacgaggtcc ttctggcgga acagcgcgtt gtcctcgatg ttgatcttgg
      361 cgtcgagcgc gacgacccgg ccctcgccgg tgacgatgag cggattgacc tcgacgaggc
      421 tcgcgtcgca ctcgaggtag agcttgtaga ggccgcgcgc gatggtctcg aactcggcga
      481 cctgcgcgcc cttcagtccc aggccgaacg ccagtgcgcg gcagtgcgat ccctgcagcc
      541 cggtggccgg gtggacctcg acacgtatga tcttctcggg cgtcttcgcg gcgacttcct
      601 cgatgtccat tccgccggcc gcggaggcga cgaatgcgat gcggccggtg tcgcgattga
      661 gcaggaggct caggtacaac tcgcgctcga tcttcgatcc ggactccacg tacacctggt
      721 tgatcgggag gccgtccgca cccgtctgct tggtcgtgag gcgcttgccg agcattccgg
      781 ccgccgccgc ggcgacgtcg tccacggtcc tgacgacctt cacgccgccg gccttgcctc
      841 gaccgccggc gtgcacctgt gccttgacca cccagacgct cccgccgagg cgtcgcgcgg
      901 cgtcggccgc accgtcgggc gtcgtggcca cgtaaccggt cggtaccggg atgccgtagc
      961 gctcgaagag ttgcttcgac tggtattcgt gcaggttcat gggcgcggcc cgctcgggta
     1021 caaggccgcg tagtttacct tcaacccgcg aaattggggt cagaccccat cttccaaaaa
     1081 atggggactg cgggaaatgg ggacattctc cttgtttcct gccggaaaaa ggagaatgtc
     1141 ctcatttccc ggcggtcgat tcaggcaaac tgccgtcgat gcgtgtcatg cccgatcccc
     1201 gccctgcccg cgccggaccg gccgacctcc cttggcgggt gctcggcctg ctcaacatct
     1261 tccggctgac ggtgccgatg gtgctgctgc tggtgttctt cttcgacgcg ccgcagcgca
     1321 gcgtcggcgc ccacagcccc ggcttgttca tcggcatctg cgccgcctac ttcgcgttcg
     1381 gcctcgtctc gatccagccg gtccagcgcc gctggccgtc ggtggagtgg ctcgcgctgt
     1441 tccaactcgc ggtggacacc gcggcgatac tcatgttcgt ccacgcgagc ggcggcgccg
     1501 cgagcgggct cggcatgctg ctcatcgtcc cggccggcgg caccgcgacc atcgtgagcc
     1561 cccgcctggc gctgttcacg gcatccatgc tggcgctcgc gttgctcatc cagaccgtgg
     1621 tggccggact cgccggcacc agccacagca ccgacttcct gatcgcggga ctcacgggag
     1681 cgagcctgtt cgccgtcacg ctgctcgcga tcccgctcgc caaccgcctg cgcgagagcg
     1741 aggcgctggt ccagcagcgc gacatcgacc tcgccaacct gagcgagctg aacgagttca
     1801 tcgtgcagca cctgcgcgag agcatcctgg tggtggac
//
LOCUS       DAOMFD010000321         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_42825_length_1783_cov_36.236690, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000321 DAOMFD010000000
VERSION     DAOMFD010000321.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42825_length_1783_cov_36.236690"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(24..779)
                     /locus_tag="PL152_11450"
     CDS             complement(24..779)
                     /locus_tag="PL152_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HQR49945.1"
                     /translation="MAGTDSNAGTGSKKVALVVGAGDATGSAIARRFAREGFVACCTR
                     RSADKLQPLLDSIRSDGGEAHGFGSDARQEQQVTKLVEHIESAVGPIEAFVFNIGANV
                     PCSILEETPRKFFKIWEMACYAGFLTGQAVAKRMAMRGRGTILFTGATASVRGSANFA
                     AFATAKHGLRALAQSMARELGPANIHVAHVVIDGAIDTAFIRDNFPDKYAAKNQDGIL
                     SPEHIADAYWYLHQQPRDAWTFEMDLRPWMERW"
     gene            940..1500
                     /locus_tag="PL152_11455"
     CDS             940..1500
                     /locus_tag="PL152_11455"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HQR49946.1"
                     /translation="MRYSSEHKDAARRRLLESGGRHAKKHGYAAAGMDALAAAAGVTT
                     GSLYRHFQGKSDLFAAIVEHELARSAALYPAPEADGPAAVARVIRNYLSVAHVEHPER
                     GCVLPSLTAEVARADSTVRAAFESGVSDVHDRMAKAIGSADKAWAILAQIVGGVMLAR
                     AMVDADARQRVLRSVESNCLSILNGG"
     gene            1516..>1783
                     /locus_tag="PL152_11460"
     CDS             1516..>1783
                     /locus_tag="PL152_11460"
                     /inference="COORDINATES: protein motif:HMM:NF016750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HQR49947.1"
                     /translation="MSRIETTACILCSRNCGLQVEIDDGHFTRVRGDEAHPNSKGYLC
                     QKAARLEHYQNHADRLRHPLKRMPDGRFERVGWDEALDDIARRLV"
BASE COUNT          288 a          580 c          617 g          298 t
ORIGIN      
        1 cgtgcgggct cgatgggtga cggtcaccag cgctccatcc aggggcgcag gtccatctcg
       61 aacgtccacg cgtcgcgcgg ttgctggtgc aggtaccagt acgcatccgc gatgtgctcg
      121 ggagacagta tcccgtcctg attcttcgcc gcgtacttgt cggggaagtt gtcgcggatg
      181 aatgccgtat cgatcgcccc gtcgatcacg acgtgggcca cgtgaatgtt ggcgggaccg
      241 agttcgcggg ccatgctctg cgccagcgcc cgcaggccat gcttcgccgt ggcgaatgcc
      301 gcgaagttcg ccgagccgcg gaccgaggcc gtcgcaccgg tgaagaggat cgtgccgcgg
      361 ccgcgcatcg ccatgcgctt cgcgacggcc tggccggtca ggaatcctgc gtagcaggcc
      421 atctcccaga tcttgaagaa cttgcgcggc gtctcctcga ggatgctgca ggggacgttg
      481 gcgccgatgt tgaacacgaa cgcctcgatc ggtccaacgg cagactcgat gtgttcgacg
      541 agcttggtga cttgctgctc ctggcgcgca tcggatccaa agccgtgcgc ctcgccgccg
      601 tcggaccgga tcgaatcgag caggggctgg agcttgtcgg cgctgcggcg cgtgcagcag
      661 gcgacgaagc cttcccgcgc gaaacgacgc gcgatcgcgg agccggttgc gtctccggcg
      721 ccgacgacga gcgcgacctt cttcgagcca gttcctgcgt tcgagtcagt tcctgccacg
      781 ggccggcccc tgcatgtgtc ctgtccacct tgttacccgt cgctgatccc agcctgtccg
      841 cgggcgcctc atgtggcact ggacgtttgt aggtaacgat cgttatccta tgaaacgagc
      901 gggtgtacgt caacgcaccc agggacaccg gaggcagcct tgcgctattc gagcgaacac
      961 aaggacgccg cgcgccgtcg gctgctggaa tccggcggtc ggcacgcgaa gaagcatggt
     1021 tacgcggccg ccggcatgga cgccctggcc gccgccgcag gcgtgacgac gggatcgctg
     1081 taccggcatt tccagggaaa gagtgatctc tttgccgcca tcgtcgagca cgaactcgcg
     1141 cgatccgcgg cgctgtaccc ggccccggag gccgacgggc ccgcggcggt ggcgcgcgtc
     1201 atcaggaact acctgagcgt ggcgcacgtc gagcaccctg agcgcggctg cgtattacct
     1261 tcgctcacgg ccgaggtggc gcgtgccgac tcaaccgtgc gagcggcttt cgagtcagga
     1321 gtgagcgacg tgcatgaccg gatggcgaaa gcgattggct cggcggacaa ggcatgggcc
     1381 atcctggcgc agatcgtggg cggcgtgatg ctcgcgaggg cgatggtcga cgcggacgcg
     1441 cggcagcggg tgctccggtc cgtggagagc aactgcctgt ccatcctcaa tggcggctga
     1501 cacgaggacg ctgcgatgag ccgaatcgag accaccgcct gcatcttgtg ctcgcgcaac
     1561 tgcggtctcc aggtcgagat cgacgacggc catttcacgc gggtccgcgg cgacgaggcg
     1621 catccgaact cgaagggcta cctctgccag aaggcggcgc gcctcgagca ctaccagaat
     1681 cacgccgacc ggcttcggca cccgctgaag cgcatgcccg atggccgttt cgagcgcgtc
     1741 ggctgggacg aggcgctcga cgacatcgcg cggcgcctgg tgg
//
LOCUS       DAOMFD010000322         1783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_42826_length_1783_cov_34.170718, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000322 DAOMFD010000000
VERSION     DAOMFD010000322.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1783
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42826_length_1783_cov_34.170718"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..>1783
                     /locus_tag="PL152_11465"
     CDS             39..>1783
                     /locus_tag="PL152_11465"
                     /inference="COORDINATES: protein motif:HMM:NF024827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiheme c-type cytochrome"
                     /protein_id="HQR49948.1"
                     /translation="MKSRPLPIVITVAVLLLVAAIAWRYGPFRSPPAATQQVETVEST
                     PQRPAGYVGSQACAACHAKESAAWQGSQHSVAMQVADERTVLGNFDNASIEHFGVTSR
                     MIRKDGGFVVRTDGADGKLADFAVTHTFGVYPLQQYLVDIGGGRLQALPIAWDARPAA
                     DGGQRWFHLYPDEKIDHTDELHWTRRQQNWNYMCADCHATDLQKNYDRKSNTFATRWA
                     ELSVGCEACHGPGSAHVDWARKAGSASHAAADSGTDDDATGKVSTPSNGLTVHFGDRR
                     AAQWHIDAATGSAVRSKPRHGDTEIEVCAQCHSRRGQFSNGYHAGERLMDHYLPVTLT
                     APLYYPDGQQRDEDYNWGSFLSSRMYSKGVTCGDCHDPHTGKPRAQGNTLCAQCHLAS
                     KYDAATHHFHKPGTPGSRCVDCHMATTTYMVVDPRHDHSFRIPRPDLTTSLGVPNACS
                     SCHREMSSTWAEAQLRKRYPHPKQGFQDFAAAFASADRGEPDATIALAQLVANQEESA
                     IARASALDRLALLGGENAVLAAEAGLKDPSPLVRHSAVGVYDSVPPEQRLAVVPLLTD
                     PLRAVRMRAARTLAPL"
BASE COUNT          306 a          658 c          567 g          252 t
ORIGIN      
        1 gactgcacaa ttgcgcgtcg tccgccgcga gccgtagcgt gaaatcgagg cccctgccga
       61 tcgtcatcac cgtcgccgtg ctgttgctcg tggcggcgat cgcgtggcgc tacggcccgt
      121 tccgatcccc gcccgcagcg actcagcagg tcgagacggt ggagtccacg ccgcaacggc
      181 cagccgggta tgtgggatcc caggcgtgcg cggcttgcca cgcgaaggaa tccgccgcct
      241 ggcagggatc gcagcactcc gtggcgatgc aggtcgccga cgaacgcacg gtgctcggca
      301 acttcgacaa tgcctcgatc gagcacttcg gagtcacgag ccgcatgatc cgcaaggacg
      361 gcgggttcgt ggtgcgcacc gatggcgcgg acggcaagct cgcggacttc gcggtcacgc
      421 acacgttcgg cgtgtacccc ctgcagcagt atctcgtcga catcggcggc ggacggctgc
      481 aggcactgcc gatcgcctgg gatgcgcggc cggccgccga cggtgggcag cgctggttcc
      541 atctctatcc cgacgagaag atcgaccaca ccgacgaact gcactggacc cggcgccagc
      601 agaactggaa ctacatgtgc gccgactgcc acgcgaccga cctgcagaag aactacgacc
      661 ggaagtccaa caccttcgcc acgcgctggg ccgagctcag cgtcggatgc gaggcctgcc
      721 atggcccggg ctccgcccac gtcgattggg cccggaaagc cgggtcggcg agccatgccg
      781 ccgcggattc gggcacggac gatgacgcaa ccggcaaggt ctcgacaccg tcgaatggcc
      841 tcaccgttca cttcggcgac cgccgcgccg cccaatggca catcgacgct gcaaccggca
      901 gcgccgtacg cagcaagccg cgccacggcg acacggaaat cgaggtctgc gcgcagtgcc
      961 actcgcgccg ggggcaattc tcgaacggct accacgccgg cgagcggctg atggatcact
     1021 acctgcccgt gaccctcacg gcaccgctct actaccccga cggccagcag cgcgacgagg
     1081 actacaactg gggctcgttc ctctcgagcc gcatgtactc gaaaggcgtc acctgtggcg
     1141 actgccacga cccgcacacc ggcaagccgc gcgcgcaggg caacaccttg tgcgcgcagt
     1201 gccacctcgc ctcgaagtac gacgcggcga cccaccactt ccacaagccc gggacgcccg
     1261 gatcgcgctg cgtcgactgc cacatggcga ccacgaccta catggtggtc gacccgcgac
     1321 acgaccacag tttccgcatt ccgcggccgg acctcacgac cagcctcggc gtgccgaacg
     1381 cctgcagttc gtgccaccgg gagatgtcct ccacctgggc cgaggcacag ctccgcaagc
     1441 gctatccgca cccgaagcag ggattccagg atttcgctgc cgcgttcgcg tccgcggatc
     1501 gtggtgaacc cgacgcgacg atcgccctgg cccagctggt cgcgaaccag gaggaatcgg
     1561 ccatcgcgcg cgcctccgcg ctcgatcgcc tggcgctgct cggcggcgag aacgccgtgc
     1621 tggctgccga agcgggactc aaggacccga gccccctcgt gcgtcattcg gccgtcggcg
     1681 tgtacgactc cgtgccaccc gaacagcgac tcgcggtggt cccgttgctc accgatccgt
     1741 tgcgtgccgt gcgcatgcgt gccgcgcgaa ccctcgctcc gct
//
LOCUS       DAOMFD010000323         1769 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_43425_length_1769_cov_33.051342, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000323 DAOMFD010000000
VERSION     DAOMFD010000323.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1769)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1769
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43425_length_1769_cov_33.051342"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..429
                     /gene="ccsA"
                     /locus_tag="PL152_11470"
     CDS             <1..429
                     /gene="ccsA"
                     /locus_tag="PL152_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQR49949.1"
                     /translation="ASFTFLALATGSIWGKPMWGTWWVWDARLTSELILLFLYLGYMA
                     LRASFDEAQRADRASAVLAIVGVINVPIIHYSVKWWSTLHQAASVTKLAKPSIALPML
                     IPLFIMIAGFQLYFGWLICRRLRAEILRRERNASWLKEVG"
     gene            433..606
                     /gene="ccmD"
                     /locus_tag="PL152_11475"
     CDS             433..606
                     /gene="ccmD"
                     /locus_tag="PL152_11475"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HQR49950.1"
                     /translation="MELREFLHMGGYAGYVWTAYGLSTLVLVLNWVSARRTEAEQREL
                     AMRRMASDKEART"
     gene            603..1058
                     /gene="ccmE"
                     /locus_tag="PL152_11480"
     CDS             603..1058
                     /gene="ccmE"
                     /locus_tag="PL152_11480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020204483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017003 - protein-heme linkage [Evidence
                     IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HQR49951.1"
                     /translation="MTPRRKRMVTVAVILVGVGIATAFALKAFQKNLLYYYSPSQIKA
                     GEAPDNRAFRVGGLVEKGSFKREPGSLEVHFTLTDYGQEIIVSYTGVLPDLFREGQGV
                     IARGKLRPDGTFVAEEVLAKHDENYMPPEVKQSLKPHAAAAQGGAETST"
     gene            1084..>1769
                     /gene="ccsA"
                     /locus_tag="PL152_11485"
     CDS             1084..>1769
                     /gene="ccsA"
                     /locus_tag="PL152_11485"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HQR49952.1"
                     /translation="MVVELGLFSLVVALLLAVAQAWFGLAGAAYGNRRWMTATRSAVT
                     GQWVFVVMSFGVLCWAFYQNDFSVLYVASNSNSELPTFYRFTAVWGAHEGSLLLWALA
                     LATWSVAVGVFSRNLPLSFAARVLGVLGVVSIGFLSFILFTSNPFERLVPAAMDGRDL
                     NPLLQDFALAVHPPTLYAGYVGSAVAFAFAVAAMLEGRLDEAWARWTRPWTTISWLFL
                     TMGIAGGSWWA"
BASE COUNT          255 a          576 c          610 g          328 t
ORIGIN      
        1 gcctcgttca cgttcctcgc gctcgccacc ggctccatct ggggcaagcc gatgtggggc
       61 acgtggtggg tgtgggacgc gcgactcacc tcggaactca tcctgttgtt cctctatctc
      121 ggctacatgg cgctcagggc gtctttcgac gaagcgcagc gcgcggatcg ggccagcgcg
      181 gtgctcgcga tcgtcggcgt gatcaacgtg ccgatcatcc actactcggt gaagtggtgg
      241 tccacgctgc accaggccgc gagcgtgacc aagctcgcga agccgtccat cgccctgccg
      301 atgctgatcc ccctgttcat catgatcgcc ggcttccagc tctatttcgg ctggctgatc
      361 tgccggcgcc tgcgggccga gatcctgcgc cgcgaacgca acgcgtcgtg gctcaaggag
      421 gttggttgaa ccatggaact gcgtgaattt ctgcacatgg gcggttacgc gggctacgtc
      481 tggaccgcgt acggcctgtc gacgctcgtg ctggtgctga actgggtgtc cgcccggcgc
      541 acggaggccg agcagcgcga gctcgccatg cggcgcatgg catcggataa ggaggcacga
      601 acatgacccc gcgtcgcaag cgcatggtga cggtggcggt gatcctggtc ggagtcggga
      661 tcgccacggc gttcgcattg aaggcattcc agaagaacct gctctattac tacagcccgt
      721 cgcagatcaa ggcgggcgag gcgccggaca accgggcctt ccgcgtcggc ggcctcgtcg
      781 agaagggcag tttcaagcgc gagcccggca gcctcgaggt gcacttcacg ctgaccgatt
      841 acggccagga gatcatcgtg agctacaccg gcgtgctgcc ggacctgttc cgggaggggc
      901 agggcgtcat cgcgcgcggc aagctccggc cggacggcac cttcgtcgcc gaggaagtcc
      961 tcgccaagca cgacgagaac tacatgccgc ctgaggtcaa gcagagcctg aagccacacg
     1021 cggccgccgc gcagggcggc gccgagacga gcacttgagc cgcgccggcc gagggaggac
     1081 cgcatggtcg tagaactggg tttgttctca ctggtcgtcg cgctgctgct tgcggtggcg
     1141 caggcgtggt tcggcctcgc aggggccgcg tacggcaacc gtcgctggat gactgcgacg
     1201 cgctcggccg tcaccggcca gtgggtattc gtcgtgatgt cgttcggcgt gctgtgctgg
     1261 gcgttctacc agaacgactt ctcggtactg tacgtcgcca gcaattccaa ttcggagctg
     1321 ccgaccttct accgcttcac ggcggtgtgg ggcgcgcacg aaggatcgct gctgctgtgg
     1381 gcgctcgcgc tcgccacctg gtccgtggcg gtcggcgtct tcagccggaa cctgccgctg
     1441 tcgttcgccg cgcgcgtgct cggcgtgctc ggcgtcgtga gcatcgggtt cctgtcgttc
     1501 atcctgttca cgtcgaaccc gttcgagcgc ctggtcccgg ccgcgatgga cggacgcgac
     1561 ctcaacccgt tgctgcagga cttcgcgctg gccgtccatc ctcccaccct ctatgcgggc
     1621 tacgtcgggt ccgcggtcgc attcgcattc gcggtcgcgg ccatgctcga gggccggctc
     1681 gacgaggcgt gggcgcgctg gacgcgcccg tggaccacga tttcgtggct gttcctcacg
     1741 atggggatcg cgggcggcag ctggtgggc
//
LOCUS       DAOMFD010000324         1758 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_43951_length_1758_cov_35.185555, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000324 DAOMFD010000000
VERSION     DAOMFD010000324.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1758
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43951_length_1758_cov_35.185555"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1758
                     /locus_tag="PL152_11490"
     CDS             <1..>1758
                     /locus_tag="PL152_11490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49953.1"
                     /translation="AERQLAAQREREAATRRALVRARTIAAVCAVLALVAAVSAVFGF
                     VATQRAKKADAQAQQARTDAEKLVGFLIEDFYAELAPTGRLDTMGKLAHEAVSYYDGL
                     PAELMTPQTQVYRGMALIREGQAQLGGKDIKGGTAKIAEARALFEKMRAGGNTSEAVT
                     YGLALSLMAPFYAWGPGGGPGSKPNDLQVAADALRPYAYAPDAARQVKITYADTLNYL
                     SHSKDSKEEGIAVCEEARKLLAGLGALDLTDLDATAAYADVTDSEARHALALGRFEQS
                     ARLEQEVYELAEKALARRPGDLREMSNRYFASDMLSHLAMSRHDYAAALRFATQSAQA
                     GEDAVRFNPTDLTTWGFWIQGRGLMASALLEQGRIQQAIDVHRGTVELARDPRAPSSL
                     NPQLEDTWYNLAILQAELGQLSAANASLQASLNSFDEFARMMPEGDPRRAIGAARLPL
                     RSARIKLLGGDAAAAYDLSTRSVRAVEAVAVAADSPASAYRAQILQISLGMVCESALR
                     VGRYADAEAASRRRLALPPSPFADDPLEDMSRRRMLLGLSIAKQGRGPEALQVLVPAM
                     DYYKRERAANASGTLFRRD"
BASE COUNT          294 a          571 c          649 g          244 t
ORIGIN      
        1 ggcggagcgg cagctcgcgg cgcagcgcga gcgcgaggcc gcgacgcgcc gcgcgctggt
       61 gcgtgcgcgg acgatcgccg ctgtctgcgc ggtgctggcg ctcgtcgctg cggtgagcgc
      121 cgtgttcggg ttcgttgcca ctcagcgggc gaagaaggcg gatgcgcagg cccagcaggc
      181 gcggaccgac gccgagaagc tggtcggctt cctgatcgag gacttctacg ccgagctcgc
      241 ccccacggga cggctcgaca ccatgggcaa gctcgcgcac gaggcagtct cgtactacga
      301 cggcctgccg gcggaactga tgacgccgca gacacaggtc taccgtggca tggcgctcat
      361 ccgtgaaggc caggcgcagc tgggcggcaa ggacattaag ggcggcacgg cgaagatcgc
      421 cgaggcgcgc gcgctgttcg agaagatgcg cgcgggcggg aacacgagcg aggcggtgac
      481 ctacgggctc gcactgtcgc tgatggcgcc gttctacgcc tggggaccgg gcggcgggcc
      541 gggatcgaaa ccgaacgacc tgcaggtggc agcggacgcg ttgcggccgt acgcatatgc
      601 gcccgacgct gcgcgacagg tgaagatcac ctatgcggac acgctgaatt acctcagtca
      661 ctccaaggac agcaaggagg agggcatcgc ggtctgcgag gaggcgcgca agctgctggc
      721 cgggttggga gcgctggatc tcacggacct cgacgcgacc gcggcctatg cggacgtgac
      781 ggattcggag gctcgccacg ccctggcgct cggtcgcttc gagcagtcgg caagactgga
      841 gcaggaagtc tacgagctgg cggaaaaggc gctggccagg cggcccggtg acctgcgcga
      901 gatgagcaac cgctacttcg cctcggacat gctcagtcac ctggcgatgt cccggcacga
      961 ctacgcggcc gcgctgcgct tcgcgaccca gtcggcacag gccggcgagg acgcggtgcg
     1021 cttcaacccg accgacctca cgacctgggg attctggatc cagggacgcg gcctgatggc
     1081 atccgccctg ctagagcagg gcaggatcca gcaggccatc gacgtgcatc gcgggaccgt
     1141 cgagctggca cgcgacccac gcgcaccgtc gagcctcaat ccgcagctcg aggacacgtg
     1201 gtacaacctg gcgatcctgc aggcggagct cggccagctg tcggcagcga acgcctcgct
     1261 gcaggccagc ctcaactcgt tcgacgagtt cgccaggatg atgccggagg gtgatccacg
     1321 gcgcgccatc ggtgcggcgc gactgccact gcgcagtgcg cgcatcaagc tgctgggcgg
     1381 ggacgcggct gcggcctacg atctgtcgac gcgatcggtg cgcgcggtgg aggccgtagc
     1441 ggtcgccgcc gacagcccgg catccgcgta tcgtgcgcag attctccaga tcagcctggg
     1501 aatggtctgc gagtcggcgt tgcgggtcgg gcgctacgcg gacgccgaag cggcgagccg
     1561 gcggcgcctg gctcttccac cgagcccgtt cgccgacgac ccgctggagg acatgtcgcg
     1621 acggcgcatg ttgctcgggc tttccatcgc gaagcagggc cgcggaccgg aagcgttgca
     1681 ggtactcgtg ccggcgatgg attactacaa gcgcgaaagg gcggccaatg cgagcgggac
     1741 gctgttccgc cgcgacta
//
LOCUS       DAOMFD010000325         1734 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_45062_length_1734_cov_34.509232, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000325 DAOMFD010000000
VERSION     DAOMFD010000325.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1734)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1734
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45062_length_1734_cov_34.509232"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..434
                     /locus_tag="PL152_11495"
     CDS             48..434
                     /locus_tag="PL152_11495"
                     /inference="COORDINATES: protein motif:HMM:NF022526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2780 domain-containing protein"
                     /protein_id="HQR49954.1"
                     /translation="MNELIKRLMKELGITKDQARGGLVALLRAAQQNMARADFDQFVV
                     DIPGADKLLKEAPPPSTLSSLAGGLGSLFGGKKSPGRWAGLAASFTELGIPIDTAKKF
                     GPIVIDYVEHHGGEDLVDKIKEALGL"
     gene            473..1249
                     /locus_tag="PL152_11500"
     CDS             473..1249
                     /locus_tag="PL152_11500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49955.1"
                     /translation="MRLAITSLALSLLLTAVAGCKPRSHELAPGMYRAVVELPGGEVP
                     FGLDVKRENGGFVLYLVNGKERVRVAATVDANGRLVAELPGSVNHLEATVSGDDLEGE
                     VTLSGAAQSMGREARAQANPGAPPSGGRATVLTFSATRGPGWRFVEEPLTDNADFSGT
                     WNITFTDDRGTTTRGVADLAQSFHTVTGTVRTAGGGDTTLAGDARDEELQLSLFDGTQ
                     AVLYHGKLDERGRLVGECWSTSSGHARYTAVRNPEATLER"
     gene            1253..>1734
                     /locus_tag="PL152_11505"
     CDS             1253..>1734
                     /locus_tag="PL152_11505"
                     /inference="COORDINATES: protein motif:HMM:NF003819.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HQR49956.1"
                     /translation="MPSFDIVSEIDAHEVTNAVDQASREVDQRFDFKGTGAKFELKEA
                     VVTLSAPADFQLKQMLEILRLKLSKRGIDVACLKVDEPVTTGQTARQVVTLRQGVDTE
                     LGKKIQRLVKDSKLKVQAAIQDKQVRVTGKSRDDLQAAIALVRKGGFDLPLQFTNFRD
                     "
BASE COUNT          302 a          583 c          597 g          252 t
ORIGIN      
        1 gctggtctgg cggccttgac gtagaatccg cgcggggagg accggcgatg aacgaactga
       61 tcaagcggct catgaaggag ctcggcatca ccaaggacca ggcgcgtggc ggcctcgtgg
      121 cgctgctgcg cgcggcgcag cagaacatgg cgcgcgccga cttcgaccag ttcgtggtcg
      181 acatcccggg cgccgacaag ctcctgaagg aggcgccgcc gccgagcacg ctcagctcgc
      241 tcgcgggagg tctcgggtcg ctgttcgggg gcaagaagag ccccggccgc tgggcgggac
      301 tcgccgcatc gttcaccgag ctcggcatcc cgatcgacac cgccaagaag ttcgggccga
      361 tcgtgatcga ctacgtcgag caccacggcg gcgaagacct ggtggacaag atcaaggaag
      421 cgctgggcct ttgaccgcgg ctcgctcgat tcaccacgcg agctgcctgc gggtgcgact
      481 cgcgatcacg tcgctggcgc tgtcgctgct gctcactgcg gtcgcgggct gcaagccgcg
      541 aagccacgag ctcgcgcccg gaatgtatcg cgccgtggtc gagctgccgg gcggcgaggt
      601 tcccttcgga ctcgatgtca agcgggagaa tggcggcttc gtgctctatc tcgtgaacgg
      661 caaggagcgc gtgcgcgtcg cggcgacggt ggacgccaac ggacgcctcg tcgccgagct
      721 gccgggcagc gtcaatcacc tggaggcgac ggtctcgggc gacgacctcg agggtgaagt
      781 gacgctctcc ggcgcggcgc aatcgatggg ccgcgaggcg cgggcccagg cgaaccccgg
      841 cgcaccacct tcaggtggcc gtgcgaccgt gctgaccttt tctgcgacac gtggcccagg
      901 ctggcgcttc gtcgaggagc cgctcaccga caacgccgac ttttccggga cgtggaacat
      961 cacgttcacg gacgaccgcg gcacgaccac gcgtggtgtg gccgacctgg cacagtcgtt
     1021 ccacacggtg accggtaccg tgcgcacggc cggcggcggc gacacaaccc tggccgggga
     1081 tgctcgcgac gaggaactgc agctctcgct tttcgacggc actcaggccg tgctgtatca
     1141 cggcaagctc gacgagcgcg gccggctcgt cggcgagtgc tggagcacgt cctcgggcca
     1201 tgcccgctac actgcggtcc gcaatcccga agccaccctc gagcgttgac ccatgccctc
     1261 cttcgacatc gtctccgaga tcgacgcaca cgaagtcacc aacgccgtgg accaggcgag
     1321 ccgcgaggtc gaccagcgtt tcgacttcaa gggcacgggc gcgaagttcg agctgaagga
     1381 ggccgtggtg acgctctccg cgccggccga cttccagctg aagcagatgc tggagatcct
     1441 gaggctcaag ctctcgaaac gcggcatcga cgtggcctgc ctgaaggtgg acgaaccggt
     1501 cacgaccggc cagaccgccc ggcaggtggt gaccctgcgc cagggcgtgg acaccgagct
     1561 cggcaagaag atccagcggc tcgtgaagga ctcgaagctc aaggtgcagg ccgcgatcca
     1621 ggacaagcag gtgcgcgtga ccggcaagag tcgcgacgac ctgcaggccg ccatcgccct
     1681 ggtccgcaag ggcggattcg acctccccct gcagttcacc aacttccgcg acta
//
LOCUS       DAOMFD010000326         1717 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_45856_length_1717_cov_26.663057, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000326 DAOMFD010000000
VERSION     DAOMFD010000326.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1717)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_45856_length_1717_cov_26.663057"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..383)
                     /locus_tag="PL152_11510"
     CDS             complement(<1..383)
                     /locus_tag="PL152_11510"
                     /inference="COORDINATES: protein motif:HMM:NF014639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HQR49957.1"
                     /translation="MYPDWLTRRNGNLLGFLACVGLIGYALFVQYGLELEPCPLCILQ
                     RISVITAGLVFLAAFLHDPGDRMARVYGVLVDLAALGGILVASRHIWIIAQPPGTVAE
                     CGASLEYMMDVLPLNEVLGKVLTGSG"
     gene            complement(392..1132)
                     /gene="phoU"
                     /locus_tag="PL152_11515"
     CDS             complement(392..1132)
                     /gene="phoU"
                     /locus_tag="PL152_11515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HQR49958.1"
                     /translation="METSDLSHHILSRFNADLEGVRSAVLQMGGLVEQQLRNGVKALH
                     GGDSRLGEEVARLDHKVNAMEVAIDDDCSRILATRGPTASDLRLIVAVIKTITDLERI
                     GDEAEKLGHIAARMGSLDRPGDRYREIRHMGDSVADMVHRALDAFARMDTDAALAVAR
                     GDRVVDEEFDAIQRQCITFMMEDPRTIRRALDVLWVARALERIGDHAKNICEYVIYMV
                     LGKDIRHLSIEDVEEQLAALRAARAGAA"
     gene            complement(1286..>1717)
                     /locus_tag="PL152_11520"
     CDS             complement(1286..>1717)
                     /locus_tag="PL152_11520"
                     /inference="COORDINATES: protein motif:HMM:NF028240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative porin"
                     /protein_id="HQR49959.1"
                     /translation="SGQSVFNVGSLPLTLFADWSQNMASGVQYDTAWAVGAALGKASN
                     YRTWEASVMYESMDKDAMFAQFIDSDFAGGVTDADGWQFKAGYAPVKNVVINATYFLN
                     TTNKDVYAVSGNVLTGLYAGPYAEGKDMNYDRFQLDFNYKF"
BASE COUNT          270 a          579 c          573 g          295 t
ORIGIN      
        1 cccgagcccg tgaggacctt gccgagcacc tcgttcaggg gcagcacgtc catcatgtat
       61 tcgaggctcg caccgcactc ggccaccgtg cccggcggtt gcgcgatgat ccagatgtgg
      121 cgggacgcga cgaggattcc gccgagcgcg gcgaggtcga cgagcacgcc gtagacccgc
      181 gccatgcggt cgcccgggtc gtgcaggaat gccgcgagga agaccaagcc cgcggtgatc
      241 accgagatcc gctgcaggat gcacagcgga cacggctcga gctcgaggcc gtactgcacg
      301 aacagcgcgt agccgatcag gccgacgcag gccaggaagc cgaggagatt gccgttacgc
      361 ctggtcagcc agtccggata catgtctcac ctcaagccgc tcccgcgcgg gccgcacgta
      421 gtgcggccag ctgctcctcg acgtcctcga tcgacaggtg gcggatgtcc ttgccgagca
      481 ccatgtagat cacgtattcg cagatgttct tggcgtggtc gccgatgcgc tcgagcgcac
      541 gcgccaccca cagcacgtcg agcgcccggc ggatcgtccg cgggtcttcc atcatgaacg
      601 tgatgcactg gcgctggatc gcgtcgaact cctcgtccac gacccggtcg ccgcgtgcga
      661 cggcgagcgc ggcatcggtg tccatgcgcg caaaggcatc gagcgcccgg tggaccatgt
      721 ctgcgacgga gtctcccatg tgccggattt cgcgataccg gtcgcccggc cggtcgagtg
      781 aacccatgcg agccgcgatg tggccgagct tctcggcctc gtcgccgatg cgctcgaggt
      841 cggtgatggt cttgatcacc gcgacgatga gccgcaggtc ggacgccgtc gggccccggg
      901 tcgcgaggat ccggctgcag tcgtcgtcga tggcgacttc catcgcgttg accttgtggt
      961 cgaggcgcgc gacttcctcg ccgagccggc tgtcaccgcc gtggagggcc ttgaccccgt
     1021 tgcgcagctg ctgctcgacc agcccgccca tctgcaggac cgccgaccgc accccttcga
     1081 ggtcggcatt gaaccggctc aggatgtggt gggagagatc ggacgtttcc atgcgggtct
     1141 ccggcggggt gcgacggtat acagcagcgc cgccccgcgg gcaagcggcc gcgcgtccct
     1201 tgcgcaccct gcgacgtgtt cgactggatc aggacggccc gggttcccgc gcgcatccct
     1261 gcacgcgggc gcctggactc acgcgtcaga acttgtagtt gaagtccagc tggaagcggt
     1321 cgtagttcat gtccttgcct tccgcgtacg ggccggcata gaggccggtc agtacatttc
     1381 cgctcacggc gtagacgtcc ttgttggtcg tgttcaggaa gtacgtcgcg ttgatgacga
     1441 cgttcttgac cggcgcgtag cctgccttga actgccagcc gtcggcatcc gtgacgccac
     1501 ccgcgaagtc ggagtcgatg aactgcgcga acatcgcgtc cttgtccatg gactcgtaca
     1561 tcacgctcgc ctcccaggtg cggtagttgg aagccttgcc gagcgcggcg ccgaccgccc
     1621 aggccgtgtc gtactgcacg cccgaggcca tgttctgcga ccagtcggcg aacagcgtca
     1681 gcggcaggct gccgacgttg aacacgctct ggccgga
//
LOCUS       DAOMFD010000327         1713 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_46056_length_1713_cov_36.667069, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000327 DAOMFD010000000
VERSION     DAOMFD010000327.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1713)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1713
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46056_length_1713_cov_36.667069"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1091
                     /locus_tag="PL152_11525"
     CDS             <1..1091
                     /locus_tag="PL152_11525"
                     /inference="COORDINATES: protein motif:HMM:NF019154.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HQR49960.1"
                     /translation="WSDMRSPSDRSSLAASVIVERCGLAKPIAVLRVRMGFPPETFAM
                     AVQPLIDAYAEYVQPTSASGYERCGGPGGQLHRGLVTALRALDHRRGQILPRNAAPEV
                     LGALGHRWTYAVFAAALLHDLGGGSPDLPGQLFERWVPASVRAWLDEDPALMAELRAV
                     LSGDASPGSAIAELVARAAARTWPLCEAPVPSGAPTVQTFDAVESAIEPAAPSPAEDS
                     PEFLDAVQTDAADPARRFMDWIRRGIVDGTLTVNTSGALVHGVEEGLLLVSPGIFRAY
                     VQHDGAGSSEKRDTAKRLQREVLRAGWHLRAEGGVNLLGYAWKQDGRADSRVHGIVIV
                     DPRRFVHPVPRINSALARVAQNEAAPDR"
     gene            1169..>1713
                     /locus_tag="PL152_11530"
     CDS             1169..>1713
                     /locus_tag="PL152_11530"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HQR49961.1"
                     /translation="MLLGPGLFLLAFPWHLLLATALATHAAWRGAAGLRTLRYRANLR
                     RRRRYEVASAEIPWATDRLFLGRGFQWDQRHTQRLFEARLPKNEPLLEAGVLGNALEV
                     FSSDSSVPAGVGGDPAIHGVEPDEAEIWMDLEDRVGHTLVLGTTRVGKTRLAELLIAQ
                     DIRRGEVVIVFDPKGDVDLLRR"
BASE COUNT          229 a          577 c          631 g          276 t
ORIGIN      
        1 catggagcga catgcgatct ccgagcgatc ggtcgtcgct cgcggcgtcc gtgatcgtcg
       61 aacgatgcgg cctcgcgaaa ccgatcgcgg tgctccgcgt ccggatgggc ttcccgccgg
      121 agaccttcgc gatggccgtg cagccgctga tcgatgccta tgcggaatac gtgcagccga
      181 cgtcggcgtc gggctacgaa cgctgtggcg gccctggcgg acagttgcac cgcggcctcg
      241 tcaccgcgct gcgcgcgctc gatcaccggc gcggccagat cctgccgcgc aacgcggcac
      301 cggaagtgct gggcgcgctc ggccatcgat ggacctacgc ggtcttcgcg gccgcgttgc
      361 tgcacgatct cggtggcgga agtccggacc ttcctgggca gctgttcgag cgctgggtac
      421 cagcgagcgt ccgggcgtgg ctcgatgaag atcccgcact gatggcggag ctgcgcgccg
      481 tgttgagcgg cgacgcgtcg ccgggcagtg cgatcgcgga gctggtggcg cgtgcggctg
      541 cccgcacctg gcccctgtgc gaggcgccgg tgccgtccgg tgcgccgacc gttcagacat
      601 tcgatgcggt ggagtccgcc atagagcctg cggcgccgtc gccagccgaa gattcgcccg
      661 aattcctgga cgctgtgcag acggatgccg cggatcccgc gcgccgtttc atggactgga
      721 ttcggcgcgg gatcgtggac gggacactga cggtgaacac gagcggcgcc ctggtgcatg
      781 gcgtcgagga ggggctgctg ctggtgtcgc ccggcatctt tcgggcgtac gtccagcacg
      841 acggcgccgg ctcgagcgaa aagcgtgaca ccgcgaagcg gctgcagcgc gaggtcctgc
      901 gggccggctg gcaccttcgg gccgagggcg gcgtgaacct gctcggctac gcgtggaagc
      961 aggacgggcg agcggacagc cgcgtccacg gcatcgtgat cgtcgatccc cgccgcttcg
     1021 tgcatccggt gccgcggatc aattcggcgc tcgctcgtgt ggcgcagaac gaggcggctc
     1081 cggatcgatg acctatccgc tggaggctca actgcggccg gcgcacgagc tccgcgccgc
     1141 tgtggtctcg gcactggcag cggcgctggt gctgctcggc ccgggactct tcctgctggc
     1201 ctttccctgg cacctgctgc tcgcgactgc gctggcgacc catgccgcat ggcgaggtgc
     1261 cgcgggcctg cggacgctgc gctatcgggc caacctgcgg cgccggcgac gctacgaggt
     1321 ggcctccgcc gagatcccgt gggccaccga tcggctcttc ctggggcgcg ggtttcaatg
     1381 ggatcaacgc cacacgcagc gcctgttcga ggcgcggctg ccgaagaacg agccgctgct
     1441 cgaagccggc gttcttggca acgcgctgga ggtcttctcg tctgactcgt cggtccctgc
     1501 cggcgtcggc ggcgatcccg ccatccacgg cgtcgagccc gacgaggccg agatctggat
     1561 ggacctggaa gaccgggtgg ggcacacgct ggtgctcggc accacgcggg tcggcaagac
     1621 gcgcctggcc gaactgctga tcgcgcagga catccgccgc ggcgaggtcg tgatcgtgtt
     1681 cgatccgaag ggcgacgtcg atctgctgcg ccg
//
LOCUS       DAOMFD010000328         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_46201_length_1710_cov_33.294260, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000328 DAOMFD010000000
VERSION     DAOMFD010000328.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46201_length_1710_cov_33.294260"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..474
                     /locus_tag="PL152_11535"
     CDS             <1..474
                     /locus_tag="PL152_11535"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR49962.1"
                     /translation="MFANSPYETKTSSERAMAQSFVNQVGNIGKILGAIVTAVFFTML
                     LVTANTMAQSVRERTNEIGVLKTLGFTNRGVLAMVLAESVLITLVGGALGLAAAWWMG
                     VQFEPVFRQYLPGFSLDAGAVMAGFAWMVGLGLAAGAVPSAQAMQLQIVEALRRD"
     gene            482..1672
                     /locus_tag="PL152_11540"
     CDS             482..1672
                     /locus_tag="PL152_11540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HQR49963.1"
                     /translation="MRPWINQLVAITAMNLRNLPQRLGTSLVAIVGIGGVVAVLVSLL
                     SMAQGFRAALELSGSEDVAIVLRGGAGDELSSSFDREQVSVIVSAPGIARDAHGAIAS
                     PEFYTIVDLPMRSTGTAANVPFRGVTDRAAAVRNDFRIIAGRMFEPGKDEVIVGRGAF
                     AQFGNVDLGRKVTWGSHEWRVVGVFECGGSVSESEVWTDLEVLRGVYRRTSSVQVVRA
                     RLTSPAALATLAGALAGDPRLNASVRGEREFYADQSRLLVTLVNYVGTTIALLMGIGA
                     LFAALNTMYSAVSSRTREIATLRALGFGAAPVVASVLLEAGALGLLGGIAGGALVYFG
                     LNGYQASTLNWASFSQIAFAFTVTPGLLVTGLLYALGLGLVGGLLPGVRAARLPVTVG
                     LREL"
BASE COUNT          217 a          537 c          646 g          310 t
ORIGIN      
        1 atgttcgcga actcgccgta cgagaccaag acctcctcgg agcgcgcgat ggcgcagtcg
       61 ttcgtcaacc aggtcggcaa catcggcaag atcctcggtg cgatcgtcac cgcggtgttc
      121 ttcaccatgc tgctggtcac ggccaatacc atggcgcagt cggtgcgcga gcgcaccaac
      181 gagatcgggg tgctcaagac gctgggattc accaatcgcg gcgtgctggc gatggtgctc
      241 gccgaatcgg tgctcatcac gctggtcggc ggcgcactgg gcctggcggc tgcgtggtgg
      301 atgggcgtcc agttcgagcc ggtattccgc cagtacctgc cgggattcag cctggacgcg
      361 ggtgcggtga tggcgggatt cgcgtggatg gtggggctgg gcctagcggc cggcgcggtt
      421 ccttcggcgc aggccatgca gctgcagatt gtcgaggcgc tgcggcgcga ctgaggaacg
      481 catgcgtccc tggatcaacc agctcgtggc gatcaccgcg atgaacctgc gcaacctgcc
      541 gcagcggctc ggcacgtcgc tcgtggcgat cgtgggaatc ggcggcgtgg tcgcggtgct
      601 cgtgtcgctg ctctcgatgg cgcagggctt ccgcgcggca ctcgagctgt ccggcagcga
      661 ggatgtggcc atcgtgctgc gcggcggcgc cggcgacgag ctgtcgagct cgttcgatcg
      721 cgagcaggtc tcggtcatcg tctcggctcc cgggatcgcg cgcgacgccc acggggcgat
      781 cgcgtcgccg gagttctaca cgatcgtgga cctgccgatg cgcagcacgg gaacggccgc
      841 caatgtcccg ttccgcgggg tgacggatcg cgctgccgcc gtccgcaacg acttccggat
      901 catcgcgggc cggatgttcg agcccggcaa ggacgaggtg atcgtgggac gcggtgcgtt
      961 cgcgcagttc ggcaacgtcg acctcggtcg caaggtgacg tggggttcgc acgagtggcg
     1021 cgtggtcggc gtgttcgagt gtggcggcag cgtctcggag tccgaggtct ggaccgacct
     1081 cgaggtgctg cgaggcgtgt accggcggac cagttccgtg caggtggtgc gggcgaggct
     1141 gacgtcgccg gcggcactcg ctacgcttgc cggtgcgctg gcgggcgacc cgcggctcaa
     1201 cgcgtcggtg cgcggggagc gtgagttcta tgccgaccag tcgcgcctcc tggtcacgct
     1261 ggtgaattac gtcggtacca ccattgcatt gctcatgggc atcggcgcct tgtttgcggc
     1321 gttgaacacc atgtattcag ccgtctcgtc gcggacccgc gagatcgcga ccctgcgcgc
     1381 gctcgggttc ggcgcggcgc cggtcgtcgc gtcggtgctg ctggaggccg gggcgctcgg
     1441 cttgctcggc ggcatcgccg gcggggcgct cgtgtacttc gggctgaacg gctaccaggc
     1501 gtcgacgctc aactgggcga gcttcagcca gatcgcgttc gcgttcacgg tcacgccggg
     1561 cctgctggtg acgggtctgc tgtacgcact cgggctcggg ctggtcggtg gcctgctgcc
     1621 cggggtgcgt gcggcgcggt tgccggtgac cgtcgggctc cgggagctct gagcaatggc
     1681 cggatccacg atgcgtcgtc gcacgcgccg
//
LOCUS       DAOMFD010000329         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_46456_length_1705_cov_36.914545, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000329 DAOMFD010000000
VERSION     DAOMFD010000329.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46456_length_1705_cov_36.914545"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1149
                     /locus_tag="PL152_11545"
     CDS             <1..1149
                     /locus_tag="PL152_11545"
                     /inference="COORDINATES: protein motif:HMM:NF012437.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HQR49964.1"
                     /translation="ENFEVLQAESGVRALEMLEAGEQVDLVLLDVMMPRLSGFEVCRE
                     IRKTRLPTQLPVVMVTARNQTQDLKTGLQVGANDYITKPVAKGELLARIRTHLNLLRI
                     NSAYSHYVPHEFLRYLSRESITDVRLGDNVEMEAAVLVSDIRDFTTMSEHMTPDENFR
                     FVNDYLAVAGPPIREHGGFVDRYSGDGILAVFPGGAGDALEAARETMRGLREFNAARA
                     RRGEPAVRIGIGLHLGRLRLGIVGEVQRRQGDIFADAVNVASRIEGMTKTYGSAVIVS
                     DAFLAGLADPERSLSSRRTLGSVQMKGRVEAVVLHEVFEFDATDLVAHKQATREAFEM
                     AQRHFEAQRYADAVATLDSILRAHGGDAAARHLRTEAAARAARESSPA"
     gene            1146..>1705
                     /locus_tag="PL152_11550"
     CDS             1146..>1705
                     /locus_tag="PL152_11550"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HQR49965.1"
                     /translation="MSARLAGSGILSRMNPDFFPLRPWSLVACVILLAAAPEVFAATA
                     AAPAAGTSWPVPVGQPLPDVVMSGLNGPPRSLSSYRGRPMIINVWASWCGPCRAEAAS
                     LERLAWSKAGGRYAIIGVSIDDYRAEALRWLGHSNATISHYIDARLTLEHLLGASTIP
                     TTVLVDAQGRVVARFRGARDWDSAESV"
BASE COUNT          257 a          578 c          605 g          265 t
ORIGIN      
        1 gagaatttcg aggtgctgca ggccgagagc ggcgtgcgcg cgctcgagat gctcgaggcc
       61 ggcgagcagg tggacctcgt gctgctcgac gtgatgatgc cgaggctctc ggggttcgaa
      121 gtctgccgtg agatccgcaa gacgcgcctg ccgacgcagc tgccggtcgt catggtcacg
      181 gcgcgcaacc agacgcagga cctgaagacc gggctgcagg tcggcgccaa cgactacatc
      241 accaagcccg tggcgaaagg cgagctgctc gcgcgcatcc ggacgcacct caacctgctg
      301 cgcatcaatt cggcctattc gcactacgtt ccgcacgagt tcctgcgcta cctgagccgc
      361 gagagcatca ccgacgtgcg actcggcgac aacgtggaga tggaggccgc ggtgctcgtc
      421 tccgacatcc gcgacttcac cacgatgtcc gagcacatga cgccggacga gaacttccgc
      481 ttcgtcaacg actacctcgc cgtcgcgggt cctccgatcc gcgagcacgg cggcttcgtc
      541 gaccgttact ccggcgacgg catcctcgcg gtcttcccgg gcggggcggg cgacgcactc
      601 gaggcggcgc gggagacgat gcgcggcctg cgcgaattca acgccgcgcg cgcccgacgc
      661 ggcgagcctg ccgtgcgcat cggcatcggc ctgcacctcg gcaggctgcg tctcggcatc
      721 gtgggcgaag tgcagcggcg ccagggagac atcttcgccg acgcggtgaa cgtcgcctcc
      781 cgcatcgaag gcatgaccaa gacctatggc agcgcggtga tcgtgagcga cgcgttcctg
      841 gcggggctcg cggatccgga gcgttcactg tcgagtcgcc ggacgctcgg cagcgtgcag
      901 atgaaggggc gggtcgaggc cgtggtgctg cacgaggtct tcgagttcga tgccacggac
      961 ctcgtcgccc acaagcaggc gacccgcgag gcattcgaga tggcgcagcg gcacttcgag
     1021 gcgcagcgct atgccgatgc ggtggcgacg ctggattcga tcctgcgggc gcacgggggc
     1081 gatgccgccg cacggcatct gcgcaccgaa gctgcggccc gtgctgctcg ggaatccagt
     1141 ccggcgtgag cgccaggctg gcgggctcgg gcatactttc acgcatgaat ccggacttct
     1201 tccccttgag gccgtggtcc ctggtcgcgt gcgtgatcct gctggccgca gcacccgaag
     1261 ttttcgcggc gacggccgct gcacccgcag ccggcacgtc gtggcccgtc ccggtggggc
     1321 agccgcttcc ggacgtggtc atgtccggat tgaacggtcc gccgcgatcg ctgtcgtcgt
     1381 atcgcggccg gccgatgatc atcaacgtgt gggccagctg gtgcgggccg tgtcgcgcgg
     1441 aagcggcctc gctcgagcgc ctggcgtgga gcaaggcggg cgggcgctac gccatcatcg
     1501 gcgtgtcgat cgacgactac cgcgccgagg ccctgcggtg gctggggcac tcgaacgcca
     1561 cgatcagcca ctacatcgat gcccggctca cgctcgagca cctgctcggc gcctcgacga
     1621 tcccgacgac ggtgctggtc gacgcgcagg gtcgcgtcgt cgcgcggttc cgcggcgcgc
     1681 gcgactggga cagcgccgag tcggt
//
LOCUS       DAOMFD010000330         1653 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_49136_length_1653_cov_31.891114, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000330 DAOMFD010000000
VERSION     DAOMFD010000330.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49136_length_1653_cov_31.891114"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..223
                     /locus_tag="PL152_11555"
     CDS             <1..223
                     /locus_tag="PL152_11555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016445439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HQR49966.1"
                     /translation="SDYGLASSVWTQDVGKALTVASRLQYGCTWINTHFMLVSEMPHG
                     GLKRSGYGKDLSMYALEDYTVVRHVMAKL"
     gene            complement(517..1473)
                     /locus_tag="PL152_11560"
     CDS             complement(517..1473)
                     /locus_tag="PL152_11560"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HQR49967.1"
                     /translation="MAVDRGRKRVAATFESAARGVMARHVPSDLSASISVGTGLRLAS
                     TPDTTLTGGSVGTCLRWMSDSGPLFVKTTEANLAWVFEAEADGLRELAAASAIRVPRV
                     LGVGATTDHAWLALEWLDLRPASPVSEAMLGEQLAAQHRETRNTFGWHRTNTIGLSVQ
                     RNEWLDDWPGFFAERRLGCQLDLAEAGGQPSRLVDRGRRLCERVPELLAGHSPVPSLL
                     HGDLWAGNHAEDGTSRPVIFDPSVYYGDREADLAMTRLFGGYGRALYAAYEAAWPLDP
                     GAGQRFTLYNLYHVLNHLNLFGGGYRAQAQAMIDSLLAEAGG"
BASE COUNT          249 a          577 c          558 g          269 t
ORIGIN      
        1 ctccgactac ggcctcgcct cctccgtgtg gacgcaggac gtcggcaagg cgctcacggt
       61 cgcgtcacgg ctgcagtacg gctgcacctg gatcaacacg cacttcatgc tggtgagcga
      121 gatgccgcac ggcggcctga agcgctcggg ctacggcaag gacctgtcga tgtacgcgct
      181 cgaggactac accgtggtgc ggcacgtgat ggcgaagctg tgacggtcgc cgtcatggct
      241 cggtaacaat cacgcggcat cctgtgcacg tcgggcgagt ccacccccca ggaccaccgg
      301 cgatcgcggg acacgctccc gtccccaggc cggtgcagga acccgcccct gcgccggcct
      361 acttttttgc atgcaccctg cccggctgcg tggccgggat cgcggtcgcg tggctgccgt
      421 ccaggatctg gccgctgccc gaaccgagtc accggacagc gacgggcggc cagcgcgcgg
      481 gcccgagcag ctagcgccca gcgcggccga acgacgctat ccgcccgcct cggccagcag
      541 gctgtcgatc atcgcctgcg cctgcgctcg atacccgcca ccgaacaggt tgaggtggtt
      601 gagcacgtgg tagaggttgt agagggtgaa gcgctggccg gcaccgggat cgagcggcca
      661 cgcggcctcg taggcagcgt agagcgccct gccgtagccg ccgaagagtc gcgtcatggc
      721 gaggtccgcc tcccgatccc cgtaatagac cgacggatcg aagatcaccg ggcgactcgt
      781 gccgtcttcg gcgtgatttc ccgcccagag atcgccgtgc agcagtgatg gcacgggtga
      841 atgcccggcg agcaattcag gcacccgctc gcaaaggcga cggccgcggt cgacgagccg
      901 ggatggttgt ccgcccgctt ccgcgaggtc gagctggcat ccgagacggc gctcggcgaa
      961 gaagccgggc cagtcgtcga gccattcgtt ccgctgaacg ctcagaccga tggtgttggt
     1021 gcgatgccag ccgaacgtgt tgcgggtctc gcggtgttgc gctgcgagtt gttcgcccag
     1081 catggcttca ctgaccggtg acgcgggacg gaggtccagc cattcgagcg ccagccacgc
     1141 atgatccgtg gtcgcgccga cgcccagcac gcgcggcacg cggatcgcgc tggccgccgc
     1201 gagctcacgc agcccgtcgg cctcggcttc gaatacccag gcgaggttgg cctccgtcgt
     1261 cttgacgaac agcggtccgg aatcactcat ccagcgcagg cacgtgccga cgcttcctcc
     1321 cgtcagcgtt gtgtccggcg tcgatgcgag cctcaggccc gtgccgaccg aaatggaggc
     1381 ggagagatcc gacgggacgt gcctggccat gactcccctg gcggcagatt cgaacgtcgc
     1441 ggcgacgcgc ttgcggccac gatccacggc cactaattta cccttctacg cggagctccc
     1501 atgcgtcgcc tgttgatcgc cgccggattg ctgctggtcg ctgccgggct gctgtggccc
     1561 tggctgtccc ggtcgccgct cggccggctc ccaggcgacc tcgtcatcga ccggccgggt
     1621 ttccggttct atgcgccgtt cgcgaccatg ctc
//
LOCUS       DAOMFD010000331         1638 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_49991_length_1638_cov_42.138345, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000331 DAOMFD010000000
VERSION     DAOMFD010000331.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1638)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1638
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49991_length_1638_cov_42.138345"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..929
                     /locus_tag="PL152_11565"
     CDS             <1..929
                     /locus_tag="PL152_11565"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HQR49968.1"
                     /translation="VGSRLGETDWWGKAPYWRHPSEQKMIQVDLDGHILGANKPAELA
                     ILADAKLFLAALAEALAPRAGRMNLDARRRKVAKYRETVADERAKLDKKLTDMYSPMN
                     TAHVAHTCQQVFPEGSVLVADGGNTAVWAMMYHEMRVPNTILSTFKFGMLGAGVAQAL
                     GAAVARPDLPVCCIIGDGAMGFHPQEVETAVRNGLRVVYVVLCDKQWGMVKMTQSLAL
                     KPVKMLVKKSLDPDENIKSDLGEIRFDDLGRSMGAHGERVADPAALKPALERALASGG
                     PAVVHVDVDPVKHMWVPGLIHFKDMHQEPKGR"
     gene            926..>1638
                     /locus_tag="PL152_11570"
     CDS             926..>1638
                     /locus_tag="PL152_11570"
                     /inference="COORDINATES: protein motif:HMM:NF013885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HQR49969.1"
                     /translation="MNAIDAVRLHFTPGSLVALDLILAFVLFGVALDMRLEDFKGIAT
                     APRGTLIGLAAHSLLLPAVAYLLTIALRPSPSIALGMILVASCPSGNISNFLVNYAHG
                     NPALSVTIAAFSMLGSILFTPFNIAFWGALNPATREILRVVDLSPLQVLGTIVVLLAI
                     PTLLGMTVAHRFPNFAQRARRPFRMLSLAFFALFVLGALVANLEFFLDYVPRVALFVF
                     LLNATALALGYYTAKLARLP"
BASE COUNT          248 a          562 c          556 g          272 t
ORIGIN      
        1 tcgtggggtc gcggctcggc gagacggact ggtggggcaa ggcgccgtac tggcggcacc
       61 cgtccgagca gaagatgatc caggtggacc tcgacgggca catcctcggc gcgaacaagc
      121 cagcggagct cgcgatcctc gccgacgcga agctgttcct cgccgcgctt gccgaggcgc
      181 ttgcgccacg tgctggccgc atgaacctcg acgcgcgccg gcgcaaggtc gcgaagtacc
      241 gcgagaccgt cgccgacgag cgcgccaagc tcgacaagaa gctcaccgac atgtactcgc
      301 cgatgaacac cgcgcacgtc gcgcacacct gccagcaggt gttcccggaa ggctcggtgc
      361 tcgtcgccga cggcggcaac acggccgtgt gggccatgat gtaccacgag atgcgcgtgc
      421 cgaacacgat cctctcgacg ttcaagttcg ggatgctcgg cgccggcgtc gcgcaggcgc
      481 tcggcgcggc cgtcgcgcgg cccgacctgc ccgtctgctg catcatcggc gacggcgcga
      541 tgggattcca tccgcaggag gtcgagaccg ccgtgcgcaa cggactgcgc gtggtgtacg
      601 tcgtgctctg cgacaagcag tggggcatgg tgaagatgac gcagagcctc gcgctcaagc
      661 ccgtcaagat gctggtgaag aagtcgctcg acccggacga gaacatcaag tccgacctgg
      721 gcgagatccg cttcgacgac ctcggccgct cgatgggagc acacggcgaa cgagtcgccg
      781 atcccgcggc gctcaagccc gcgctcgagc gtgcgctggc gagcggcggc ccggcggtcg
      841 tgcacgtcga cgtggacccg gtgaagcaca tgtgggtgcc gggcctcatc cacttcaagg
      901 acatgcacca ggagccgaag gggcgatgaa cgcgatcgac gcggtccggc tgcacttcac
      961 gccgggatcg ctcgtcgcgc tggacctgat cctcgccttc gtgctgttcg gcgtggcgct
     1021 ggacatgcgg ctcgaggact tcaagggcat cgccaccgcg ccgcggggca cgctgatcgg
     1081 gctcgcggca cactcgctgc tgctgccggc ggtggcttac ctgctgacga ttgcactgag
     1141 gccgtccccg agcatcgcgc tcggcatgat cctcgtcgcg tcctgcccga gcgggaacat
     1201 ctcgaacttc ctggtcaact acgcgcacgg caacccggcg ctgtcggtga cgatcgcggc
     1261 gttctcgatg ctgggctcga tcctgttcac gccgttcaac atcgcgttct ggggcgcgtt
     1321 gaaccccgca acgcgcgaga tcctgcgcgt cgtcgacctg agcccgctgc aggtgctggg
     1381 cacgatcgtg gtgctgctcg cgatcccgac cctgctcggg atgacggttg cccatcgctt
     1441 cccgaacttc gcgcaacgcg cgcggcggcc gttccgcatg ctgtcgctcg cgttcttcgc
     1501 gctgttcgtg ctcggcgcgc tggtcgccaa cctggagttc ttcctcgact acgtgccgcg
     1561 cgtggcgctg ttcgtgttcc tgctgaatgc gacggcactg gcgctcggtt actacaccgc
     1621 gaagctggcg cggctgcc
//
LOCUS       DAOMFD010000332         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_50326_length_1632_cov_35.299937, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000332 DAOMFD010000000
VERSION     DAOMFD010000332.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50326_length_1632_cov_35.299937"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="PL152_11575"
     CDS             <1..914
                     /locus_tag="PL152_11575"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HQR49970.1"
                     /translation="DLFEPTPAEEKAVESLLTVDVPTRDEMKEIETSNRLYEEGNAVY
                     MTATVGSKLDSPTPESSAITFILANDRLITNRYVDPTPVRRFMQYVERSPAACTSSAT
                     LLAGLLESFVERIADLLERVQMELDSVSHTIFPRGNGTAPAGGKATTLPSSSNLQAMI
                     LKIGSNGDLVSKARESLVSFQRLLMYVQQATTVTLSQDQRGRFKSTLRDVQSLSDHAT
                     FLGNKIQFLLDAVLGLINLEQNNIIKIFSVAAVMLMPPTLVASVYGMNFHFMPELDEH
                     WGYPFALLAMVVSAVVPYLYFRRKGWL"
     gene            921..1538
                     /locus_tag="PL152_11580"
     CDS             921..1538
                     /locus_tag="PL152_11580"
                     /inference="COORDINATES: protein motif:HMM:NF013933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HQR49971.1"
                     /translation="MTPAAFLTISATVLVAAVTPGPAATAIVARAIADGLRPALALSA
                     GVLTGDLLFLTLAAAGMAAAAHSMGEFFTVLRWAGAAYLAWQGVSLWRARPHAATVRA
                     SAHEGHFWRNYGAGLLLMMGHVQAILFYAALLPGVVNFAAFTTADVLVVAAMLVVIIG
                     GVNAGWAFVGARARGFFSDERALRAVNRVAGTLMLAAAALVATRV"
BASE COUNT          239 a          572 c          549 g          272 t
ORIGIN      
        1 ccgacctgtt cgagccgacg ccggccgagg agaaggccgt cgagtcgctg ctcacggtcg
       61 acgttccgac gcgcgacgag atgaaggaga tcgagacgtc caatcgactc tacgaggagg
      121 gcaacgcggt ctacatgacg gccaccgtcg gcagcaagct cgactcgccc acccccgagt
      181 cgagcgcgat cacgttcatc ctcgccaacg accgcctgat caccaaccgc tacgtcgacc
      241 cgacgccggt ccggcgcttc atgcagtacg tggagcgctc gcccgccgcg tgcacgtcgt
      301 cggcgacgct gctcgcaggg ctgctcgaat cgttcgtcga gcgcatcgcc gacctgctcg
      361 agcgcgtgca gatggaactc gactcggtct cgcacacgat cttcccgcgc ggcaacggca
      421 cggcgcccgc cggcggcaag gccacgaccc tgccgagcag cagcaacctc caggcgatga
      481 tcctgaagat cggttccaac ggcgacctgg tgtcgaaggc gcgcgagagc ctggtgagtt
      541 tccagcgcct gctcatgtac gtgcagcagg cgacgaccgt gacgctgtcg caggaccagc
      601 gcggccgctt caagtccacg ctgcgcgacg tgcagtcgct ctccgaccac gcgaccttcc
      661 tcggcaacaa gatccagttc ctgctcgacg cggtgctcgg gctcatcaac ctcgagcaga
      721 acaacatcat caagatcttc tcggtggcgg ccgtgatgct gatgccgccg acgctggtgg
      781 cgagcgtgta cggcatgaac ttccacttca tgccggagct cgacgagcac tggggctatc
      841 cattcgccct gctcgcgatg gtcgtgtcgg ccgtcgtgcc ctacctctat ttccgtcgca
      901 agggctggct gtagccttcg atgaccccgg ctgcgttcct gacgatctcc gcgacggttc
      961 tcgtcgccgc ggtgacgccg ggacccgcgg cgacggcgat cgtcgctcgc gccatcgcgg
     1021 acggactgcg gcccgcgctc gcgctctcgg ccggggtgct gaccggcgac ctgctgttcc
     1081 tcacgctggc cgcggccggc atggcggcgg ccgcgcactc gatgggcgag ttcttcacgg
     1141 tgctgcgctg ggccggcgcg gcctacctcg cgtggcaggg cgtctcgctg tggcgagcac
     1201 ggccacacgc ggcgacggtg cgtgcttccg cgcacgaggg tcacttctgg cgcaactacg
     1261 gcgcggggct gctgctcatg atgggccacg tgcaggcgat cctcttctac gcggcgctgc
     1321 tgccgggcgt ggtgaacttc gcggccttca cgaccgccga cgtgctcgtc gtcgccgcca
     1381 tgctggtggt catcatcggc ggcgtgaacg cgggctgggc gttcgtcggc gcgcgcgccc
     1441 gcgggttctt ctccgacgag cgcgcgctcc gcgcggtgaa ccgcgtcgcc ggcacgctga
     1501 tgctcgcggc ggccgcgctg gtggcgactc gggtgtagga tggcggcatg gcgaaaccag
     1561 cgatcaacaa catggccgtc ttctgcgact tcgagaacgt ggcgctcggc gtgcgcgacg
     1621 cgaagtacgc ga
//
LOCUS       DAOMFD010000333         1623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_50844_length_1623_cov_33.029974, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000333 DAOMFD010000000
VERSION     DAOMFD010000333.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50844_length_1623_cov_33.029974"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1623)
                     /locus_tag="PL152_11585"
     CDS             complement(<1..>1623)
                     /locus_tag="PL152_11585"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: protein motif:HMM:NF006914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004591 - oxoglutarate dehydrogenase
                     (succinyl-transferring) activity [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HQR49972.1"
                     /translation="TLREIVAQLKHIYCGTIGAEFAHVSNETERLWLQDRFQVGRMQQ
                     HYSPDARKTILHGLTAAEGLERYLATKYPAQKRFSLEGADSLIPLLEDLIQASGAHGV
                     EDIVIGMAHRGRLNVLVNVLGKVPEVLFSEFEGKYDLGKLKGSGDVKYHKGFSCDVRT
                     PSGNVHVALAFNPSHLEVVDPVVEGSVRARQERRGDAAGDKVMAVLIHGDSAFAGQGV
                     IQETLQMSQARAFYTGGTVHVIVNNQVGFTTHDPRDTRSTLYCSDVAKMIEAPILHVN
                     GDDPESVLFAGRLALEYRMRFHKDIVIDLVCYRRLGHNEADEPAATQPVMYATIRAKP
                     TTRQRYAEKLATDGVLAAGEADAMAEEYRHGLDDGDGRPQARNVIGMVGNVHTVDWSR
                     YHDVDWTEQVRTAVQLATLKDLGMRATTFPEGFTLHRQVQRIMDDRRRMAAGEVLLDW
                     GFAETLAYATLLDEGYEVRLTGQDSGRGTFFHRHAVGHDQKTGASYIPLRHLKAGQPR
                     FRVTDSLLSEEAVLGFEYGYATTDPNCLVIWE"
BASE COUNT          239 a          555 c          544 g          285 t
ORIGIN      
        1 cttcccagat cacgaggcag ttcgggtccg tcgtcgcgta gccgtactcg aagccgagca
       61 cggcttcctc ggagagcagc gaatccgtca cgcggaagcg cggctggccg gccttgaggt
      121 gacgcagcgg gatgtaggac gcgcccgtct tctggtcgtg accgaccgcg tggcgatgga
      181 agaacgtgcc gcggccgctg tcctggccgg tgaggcggac ttcgtagccc tcgtcgagca
      241 gcgtcgcgta ggccagcgtc tccgcgaatc cccagtcgag caggacctcg ccggcggcca
      301 tgcggcgacg atcgtccatg atcctctgca cctggcgatg caacgtgaaa ccctcgggga
      361 acgtggtggc tcgcatgccg aggtccttca gcgtcgcgag ctggaccgca gtgcgcacct
      421 gctcggtcca gtcgacgtcg tggtaccggc tccagtccac cgtgtgcacg ttgccgacca
      481 tcccgatcac gttgcgcgcc tgcggtcgtc cgtcgccgtc gtcgaggccg tgccggtact
      541 cctcggccat cgcatctgcc tcgccggccg cgagcacgcc atcggtcgcg agtttctcgg
      601 cgtagcgctg ccgtgtcgtg ggcttcgcac ggatcgtcgc gtacatcacc ggctgcgtcg
      661 ccgcgggctc gtcggcctcg ttgtggccga gccgccggta acacacgagg tcgatcacga
      721 tgtccttgtg gaaccgcatc cggtactcga gggccaaccg gcctgcgaac aggacggact
      781 ccgggtcgtc gccgttcacg tgcaggatcg gcgcctcgat catcttcgcg acgtcgctgc
      841 agtagagcgt cgagcgcgtg tcccgcggat cgtgcgtggt gaatcccacc tggttgttga
      901 cgatcacgtg gaccgtgccg ccggtgtaga aggcgcgcgc ctgcgacatc tgcagcgttt
      961 cctggatcac gccctgcccg gcgaacgcgg aatcgccgtg gatcagcacc gccatcacct
     1021 tgtcgccggc ggcatcgcca cggcgttcct gccgggcgcg caccgagccc tcgacgaccg
     1081 gatccacgac ctcgaggtgc gaggggttga aggcgagcgc gacgtgcacg ttgccggacg
     1141 gcgtgcgcac gtcgcaggag aaacccttgt ggtacttgac gtcgcccgag cccttgagct
     1201 tgcccaggtc gtacttgccc tcgaactccg agaacagcac ctcgggcacc ttgccgagca
     1261 cgttcaccag cacgttcagg cggccgcggt gcgccatgcc gatcacgatg tcctcgacgc
     1321 cgtgcgcgcc gctcgcctgg atcaggtcct cgaggagcgg gatcaggcta tccgctccct
     1381 cgagcgagaa gcgcttctgg gcggggtact tggtcgcgag ataacgctcg agtccctcgg
     1441 ccgcggtaag cccgtgcagg atcgtcttgc gggcgtcggg cgagtagtgc tgctgcatcc
     1501 gccccacctg gaagcggtcc tgcagccaca ggcgctcggt ctcgttcgac acgtgggcga
     1561 actcggcgcc aatcgtgccg cagtagatgt gcttcaactg cgcgacgatc tcgcgcagcg
     1621 tga
//
LOCUS       DAOMFD010000334         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_51063_length_1619_cov_30.507033, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000334 DAOMFD010000000
VERSION     DAOMFD010000334.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51063_length_1619_cov_30.507033"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(55..531)
                     /locus_tag="PL152_11590"
     CDS             complement(55..531)
                     /locus_tag="PL152_11590"
                     /inference="COORDINATES: protein motif:HMM:NF019932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HQR49973.1"
                     /translation="MNRTICALLAGLLVSCAASAADLSPEEQAVWQLEETYWRAVQAG
                     DVDAYLALWHPNFVGWPCFAWEPADVSQIGDWVREIRDKHWKVTYQLKPRAVRVTGDV
                     ATALYAAEYVTDYGDGTRSGAGIWRKFTHTWIRQDGRWRIITGMCAAQEPLKTPRE"
     gene            complement(591..1097)
                     /locus_tag="PL152_11595"
     CDS             complement(591..1097)
                     /locus_tag="PL152_11595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49974.1"
                     /translation="MDLATVVDVSQIVAALTVVGGTLFAVLQLREIKQQRQEAAALDL
                     LRAFMGPEFAEAMAIVTSLPDGLTVDGLRRAGPDAEKAATLMCTTFEAMGVLVHRRIA
                     PLSLVQDIAGGIILVTWRRLEPWLTELRIEQGNPSDSEWFQWLAEQLERRKTEKQPAY
                     LLHREWQP"
     gene            complement(1160..>1619)
                     /locus_tag="PL152_11600"
     CDS             complement(1160..>1619)
                     /locus_tag="PL152_11600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49975.1"
                     /translation="TLRQPNVLATLSGRYGLNGPLYPGKWLSKSFRPVSDRSGRGYNS
                     FGHFGRVVQRFPMATLIAPSRYDRRPAYQLVYRAYHSLFGALHMVDEVRRLEAGRYLG
                     IGTFGFTDAQRRVPYPFLLTGPVATCRDDIGRERAGFRIESEVPALSGRA"
BASE COUNT          254 a          591 c          529 g          245 t
ORIGIN      
        1 ggccgagcgc cgcagcaaag cgctcggccc gtgtatttcc ggggccgccg cccctcactc
       61 acgcggcgtc ttgagcggct cctgcgcggc gcacatgccg gtgatgatcc gccagcggcc
      121 gtcctgcctg atccaggtgt gcgtgaactt ccgccagatc cccgcgccgc tgcgcgtgcc
      181 gtcgccgtag tccgtcacgt actccgcggc atacagcgcg gtggcgacat cgcccgtcac
      241 ccgcaccgca cgcggcttga gctgataggt gaccttccag tgcttgtcgc gaatctcgcg
      301 cacccagtcg ccgatctggc tcacgtccgc cggctcccag gcgaagcacg gccagcccac
      361 gaagttcggg tgccacagcg ccaggtaggc gtccacgtcg cccgcctgca cggcccgcca
      421 gtacgtctcc tcgagctgcc agacggcctg ctcctccgga ctgagatccg cggcggacgc
      481 cgcacaggac accaggagcc ctgcgagcag ggcgcaaatc gttcggttca tgtggatgcg
      541 acgcctcccc gatgcgtgct cgtccgagga tatcagctcg acgcccgctc ctacggctgc
      601 cattcccggt gcagaaggta cgccggttgc ttctcggtct tgcgtcgctc gagctgctcg
      661 gccagccact ggaaccactc ggagtcggac gggttgccct gttcgatgcg caactcggtc
      721 agccacggct cgagccggcg ccacgtgacc aggatgatgc cgcccgcgat gtcctgcacg
      781 agcgacagcg gcgcaatccg gcgatgcacg agtacgccca tggcctcgaa cgtcgtgcac
      841 atgagcgtcg ccgccttttc ggcgtcgggt ccggcgcgcc gcaggccatc gaccgtcagg
      901 ccgtcgggaa ggctcgtgac gatcgccatg gcctcggcga actccggtcc catgaacgcg
      961 cgcagcaggt ccagggcggc cgcctcctgg cgctgctgct tgatctcgcg cagctgcagc
     1021 acggcgaaca gcgtgccgcc gacgaccgtc agcgcagcga cgatctgcga cacatcgacg
     1081 acggttgcga ggtccatggt caacgtctcc tgggcgagat gagcgcccat tggtcccgcg
     1141 cgaggtccgc gtcttgcggt caggcccggc ccgacagcgc cggcacctcg ctctcgatcc
     1201 tgaaccccgc ccgctcccgg ccgatgtcgt cgcgacacgt cgcgaccggc cccgtcagca
     1261 ggaacggata gggtacgcgg cgctgcgcgt ccgtgaagcc gaaggttccg atgccgagat
     1321 accgccccgc ctcgagccgc cgcacctcgt ccaccatgtg cagggcgccg aacagggaat
     1381 ggtacgcgcg gtagacgagc tgatacgccg ggcgacgatc gtagcgcgac ggcgcaatga
     1441 gcgtcgccat cgggaagcgc tgcaccacgc ggccgaagtg cccaaacgag ttgtagccgc
     1501 ggccactgcg gtcgctcacc ggccggaacg acttggacag ccatttcccc ggatacagcg
     1561 gaccattgag cccgtagcgc ccggacaggg tcgcgaggac attgggctgc cgaagggtt
//
LOCUS       DAOMFD010000335         1599 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_52224_length_1599_cov_25.551166, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000335 DAOMFD010000000
VERSION     DAOMFD010000335.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1599)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1599
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52224_length_1599_cov_25.551166"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..215
                     /locus_tag="PL152_11605"
     CDS             <1..215
                     /locus_tag="PL152_11605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49976.1"
                     /translation="FIVAAVALGVALGAFARSRRHAARRAAEERERARRRRRTLPLVS
                     ANVRGLPQEREDLWKADPPVRKDRVA"
     gene            complement(222..623)
                     /locus_tag="PL152_11610"
     CDS             complement(222..623)
                     /locus_tag="PL152_11610"
                     /inference="COORDINATES: protein motif:HMM:NF016701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HQR49977.1"
                     /translation="METPDLAVHRGGCHCGAVAFEVDAPTRVTVHDCNCSMCSMTGYL
                     HLIVPASRFRLVRGAEMLETYTFNTGTAQHLFCRRCGIKSFYVPRSNPDGFSVNLRCV
                     DRSTFERVDVEPFDGRNWEDAGESLAHLSRT"
     gene            complement(632..>1599)
                     /locus_tag="PL152_11615"
     CDS             complement(632..>1599)
                     /locus_tag="PL152_11615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HQR49978.1"
                     /translation="AIVNPAAGMPRSRGSWDAVERSLRAARVPLEVVRTECEHDGVEI
                     ARRAVREGRRRLLAVGGDGSVNDVVNGVMDAGLADTREVTLAVAPTGTGNDWARSLGI
                     GREPESVTAAIAAGRTMLHDVGIITGPSVTGYMERANGTELPHRHWYINVAGAGYDAY
                     VTARVPRPVPSAFTYLGIALRGLLSYQSPQFRVDADGTRFDQRMLLVFAANARYCGNR
                     MHVAPDALLDDGMLDVVAIEEVGLLRALPKIVKLYRGRLYGDPVVRHVRCARVRIESD
                     PPAEVQADGQMAGHTPAEFSLERQALTVIVPPEGPCPPRRGPHTA"
BASE COUNT          247 a          559 c          562 g          231 t
ORIGIN      
        1 cattcatcgt ggcagcggtc gcgcttggcg tcgcgctcgg cgcgttcgca cggtccaggc
       61 gacatgcggc gaggcgtgcg gcggaggaac gcgagcgggc gcgtcgccgc cgtcgcacgt
      121 tgccgctcgt gtcggcgaac gtgcggggcc tgccgcagga acgcgaggac ctctggaagg
      181 cagacccacc ggtccgcaag gaccgcgtgg cctgagcgcg cctacgtgcg cgagaggtgc
      241 gccagggact cgcccgcgtc ttcccagttg cgcccgtcga acggctccac gtccacgcgc
      301 tcgaaggtcg agcgatcgac gcagcgcagg ttgacgctga acccgtccgg attcgaccga
      361 ggcacgtaga aggacttgat cccgcagcga cggcagaaga ggtgttgcgc ggtgcccgtg
      421 ttgaacgtgt aggtctcgag catctccgcg cctcgcacca gccggaagcg gctcgccggc
      481 acgatgaggt gcaggtagcc ggtcatcgag cacatcgagc agttgcagtc gtgcacggtc
      541 acgcgggtcg gcgcatccac ttcgaaggcg acggcgccgc agtggcagcc gccacggtga
      601 acggcgagat ccggcgtttc cattacacga ttcacgctgt gtgcggaccc ctgcgcggcg
      661 ggcaggggcc ttccggcggc acgatcaccg tcagggcctg ccgctccagc gaaaattccg
      721 cgggcgtgtg tccggccatc tgcccatccg cctggacctc ggccggcgga tcggactcga
      781 tgcgcacccg cgcgcagcgg acgtggcgca cgacgggatc gccgtagaga cggccgcggt
      841 agagcttcac gatcttcggc agcgcgcgca gcaacccgac ctcctcgatt gcaaccacgt
      901 cgagcattcc gtcgtcgagc agtgcgtcgg gcgccacgtg catgcgattg ccgcagtacc
      961 gcgcattcgc ggcgaacacg agcagcatgc gctgatcgaa ccgcgtcccg tcggcgtcga
     1021 cccggaattg cggcgactga tacgacagca ggccgcgcag cgcgatgccg aggtaggtga
     1081 atgccgacgg gaccgggcgt ggcacccgtg ccgtcacgta ggcgtcgtag cccgcgcccg
     1141 cgacattgat gtaccagtgt cgatgcggca actccgtgcc gttcgcgcgc tccatgtagc
     1201 cagtgaccga gggtcccgtg atgatgccga cgtcgtggag catcgtgcgc cccgccgcga
     1261 tcgcggccgt cacggactcg ggctcgcgcc cgatcccgag ggatcgggcc cagtcgttgc
     1321 cggtgccggt cggtgcgacg gcgagcgtga cttcgcgcgt gtcggccagg ccggcgtcca
     1381 tcacgccgtt gacgacgtcg ttcaccgatc cgtcgccgcc caccgcgagc aaccgacggc
     1441 gaccctcccg gaccgcccgc cgcgcaatct cgacaccgtc gtgctcgcac tccgtgcgaa
     1501 cgacctcgag gggaactcgc gcagccctca gcgaccgttc gacagcgtcc cagctgccgc
     1561 gcgagcgcgg catgccggcg gcggggttga cgatggcga
//
LOCUS       DAOMFD010000336         1598 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_52273_length_1598_cov_40.034349, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000336 DAOMFD010000000
VERSION     DAOMFD010000336.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1598)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1598
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52273_length_1598_cov_40.034349"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1119
                     /gene="gcvPB"
                     /locus_tag="PL152_11620"
     CDS             <1..1119
                     /gene="gcvPB"
                     /locus_tag="PL152_11620"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HQR49979.1"
                     /translation="LQEMLKEVTGMRGVSLTPMAGAQGEFAGVAMIRAYHLARKDPGR
                     TEIIVPDAAHGTNPATATMCGCTVKEVASLPNGDIDLDALRAAVGPHTAGIMLTNPST
                     LGVFERRIREVAGIVHAAGGLLYYDGANLNAILGKVRPGDMDFDVIHMNLHKTFSTPH
                     GGGGPGSGAVGVGERLLPFLPVPVVGRDGDRYRWLDERDLPQTIGRLSAFMGNTGVLL
                     RAYVYMRMLGRDGMRRVAEFATLNANYLMKRLADAGFELAYPQRRAGHEFIVTLRREA
                     RDYGTSAMDFAKRLLDYGFHAPTTYFPLLVPECLLIEPTETESKQELDAFVDAMVAIR
                     EEARTDPAKLKGAPYTLPVRRLDDVRAAKQLDLAWRPA"
     gene            1119..1487
                     /locus_tag="PL152_11625"
     CDS             1119..1487
                     /locus_tag="PL152_11625"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004143 - diacylglycerol kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HQR49980.1"
                     /translation="MRDGVRPTGLRRLWLATTHTMRGFRNCYRSEAAFRQETWLAIVL
                     VPAGLWLGRSGLERALLVGTVVVVLIVELLNTGIELVVDRIGTEHHHLSGFAKDVASA
                     AVFCSLVLLGSTWVLVLIFP"
BASE COUNT          237 a          570 c          537 g          254 t
ORIGIN      
        1 ctgcaggaga tgctgaagga agtcaccggc atgcgcggcg tgtcgctcac gcccatggcg
       61 ggcgcgcagg gcgagttcgc cggcgtggcg atgattcgcg cgtaccacct cgcgcgcaag
      121 gatcccggac gcaccgagat catcgtgcct gacgccgcgc acggcaccaa cccggcgact
      181 gcgacgatgt gcggctgcac ggtcaaggaa gtggcgtcgc tgccgaacgg cgacatcgac
      241 ctcgacgcat tgcgcgccgc ggtcggaccg cataccgccg ggatcatgct gaccaatccc
      301 tcgacgctcg gcgtgttcga gcggcgcata cgcgaggtcg ccggcatcgt ccacgcggca
      361 ggcgggcttc tctactacga cggcgccaac ctgaacgcga tcctgggcaa ggtccggccg
      421 ggcgacatgg atttcgacgt catccacatg aacctgcaca agaccttctc gacgccgcac
      481 ggcggcggcg gcccgggctc gggcgccgtg ggcgtgggcg agcgcctgct gccgttcctg
      541 ccggtgcccg tcgtgggccg cgatggcgac cgctaccgct ggctcgacga gcgcgacctg
      601 ccgcagacca tcggccggct ctccgcgttc atgggcaaca ccggcgtgct gctgcgcgcc
      661 tacgtgtaca tgcgcatgct cggccgcgac ggcatgcggc gcgtggcgga gttcgcgacg
      721 ctcaatgcca actacctgat gaagcgactc gcggacgccg ggttcgagct tgcgtacccg
      781 cagcggcgcg cagggcacga attcatcgtc accctgcgcc gggaggcccg cgactacggc
      841 acgagcgcca tggacttcgc caagcgcctg ctcgactacg ggttccacgc cccgacgacc
      901 tatttcccgc tgctcgtccc cgagtgcctg ctcatcgagc ccaccgagac ggagtcgaag
      961 caggagctcg acgcgttcgt ggacgcgatg gtcgcgattc gcgaggaagc ccgcaccgac
     1021 ccggccaaac tcaagggagc gccctacacg ctgcccgtgc gacgcctcga cgacgtgcgc
     1081 gcggccaagc agctcgacct cgcgtggagg ccggcctgat gcgtgacggc gtgcgtccca
     1141 cgggcctgcg acggctctgg ctcgcgacga cgcacaccat gcgcggcttc cggaattgct
     1201 atcgcagcga ggcggcgttc cgccaggaga cgtggctcgc gatcgtgctg gtgccggccg
     1261 gcctgtggct cggccgcagc ggcctggagc gcgcgctcct ggtcgggacg gtcgtcgtcg
     1321 tgctcatcgt cgagctgctc aacacgggca tcgagctcgt ggtggaccgc atcggcacgg
     1381 agcaccacca cctctcggga ttcgccaagg acgtggcctc cgccgccgtg ttctgctcgc
     1441 tcgtgctgct cggctcaacc tgggtactgg tgctcatctt tccatgacag cgctcatctg
     1501 cggctccatc gcgttcgaca ccgtgatggt gtttcccggg cggttccggg accacatcct
     1561 gccggaccgc atccacctgc tgaacgtgtc gttcctcg
//
LOCUS       DAOMFD010000337         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_52693_length_1591_cov_40.760417, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000337 DAOMFD010000000
VERSION     DAOMFD010000337.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52693_length_1591_cov_40.760417"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..624)
                     /locus_tag="PL152_11630"
     CDS             complement(<1..624)
                     /locus_tag="PL152_11630"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HQR49981.1"
                     /translation="MKPAEFDLDSQSARVRAALEHVQALEPARATERSLELASLLAQL
                     RADEDVVLAGLLMPWLEAGSLDDARAQDYFGETAVRLAREVERVGSIGIPPEWNPGHP
                     MKPQQAEGLRKLLLALASDVRLVLLRLVLQLVRMRNLKEASAAEQRWAALETREIYAP
                     LANRLGIWHIKWELEDLAFRYSEPEEYKRIAGWLKSKRAERERYIEDV"
     gene            676..1254
                     /gene="ruvC"
                     /locus_tag="PL152_11635"
     CDS             676..1254
                     /gene="ruvC"
                     /locus_tag="PL152_11635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HQR49982.1"
                     /translation="MPRLSRVGRRWAIPSRDRAASGESVRILGLDPGSVATGFGIIDW
                     GEGEARYVTSGAIRTSGGEFPPRLRQIFEGVHELVREYRPAEVAVERVFMHRNADSAL
                     KLGQARGAALCAVFAATPEVFEYAPREVKLAVVGQGAAQKEQVQLMVRTLLRLTGELG
                     PDAADAIGIALCHAYSRQARVAIARSAGVDGR"
     gene            1251..>1591
                     /gene="ruvA"
                     /locus_tag="PL152_11640"
     CDS             1251..>1591
                     /gene="ruvA"
                     /locus_tag="PL152_11640"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020649815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HQR49983.1"
                     /translation="MIGFLRGRIAGKHPPTLVVDVGGVGYELEAPMSTFYGLPAVGAE
                     VILHTHLVVREDAHVLFGFGTERERSLFRELIKVSGVGPRIALAILSGVSVDEFQRCV
                     EAEDVASLVRVP"
BASE COUNT          219 a          527 c          582 g          263 t
ORIGIN      
        1 gacgtcctcg atgtagcgct cgcgctcggc gcgcttcgac ttgagccagc ccgcgatgcg
       61 cttgtattcc tccggttcgc tgtagcggaa ggcgaggtcc tcgagttccc acttgatgtg
      121 ccagatcccg agccggttgg cgagcggtgc gtagatttcc cgggtctcga gcgcggccca
      181 gcgctgctcg gcggcggacg cctccttgag gttacgcatc cgcaccagct gcaggaccag
      241 ccgcagcagc accaggcgca cgtcggaggc cagcgcgagc aacagcttgc gcaggccctc
      301 ggcctgctgc ggcttcatcg ggtgtcccgg attccattcg ggcggaatgc cgatgctgcc
      361 gacgcgctcg acttcccgcg cgagtcgcac ggccgtctcg ccgaagtagt cctgcgctcg
      421 cgcatcgtcg agggagccgg cctcgagcca gggcatcagc aagccggcca gcaccacgtc
      481 ctcgtccgcg cgcagctggg cgagcagcga tgcgagttcc aggctgcgct cggtggcgcg
      541 ggccggctcg agggcctgca cgtgctcgag cgccgcccgg actcgggcgg actgggaatc
      601 gagatcgaat tctgccggct tcacagcgtg cgttatcata cgccgccgtc attgcgtctc
      661 ccgcggggtg aggacttgcc caggctcagt cgtgttggcc ggcgctgggc gatcccgtcg
      721 cgggaccgcg cggcgagcgg cgagtcggtt cgcatactcg gactcgatcc cggctcggtc
      781 gcgaccgggt tcggcatcat cgactggggc gagggcgagg cccgctacgt cacgagcggc
      841 gcgattcgca ccagcggcgg cgagtttcct ccgcggctgc gccagatctt cgaaggcgtg
      901 cacgaactcg tgcgcgagta ccggcctgcg gaagtggcag tcgagcgggt attcatgcat
      961 cgcaatgccg acagcgcact caagctcggc caggcgcgcg gcgcggcgct gtgcgccgtg
     1021 ttcgcggcga cgccggaggt gttcgaatac gcgccgcgcg aggtcaagct cgcggtcgtg
     1081 gggcagggtg ccgcgcagaa ggagcaggtg cagttgatgg tcaggacgtt gctcaggctc
     1141 acgggcgaac tcgggcccga cgcggccgac gcgatcggca tcgcgctgtg ccacgcctat
     1201 tcgcgccagg ccagggtcgc gatcgcccgc agtgccgggg tcgacggacg atgatcggat
     1261 tcctgcgcgg ccggatcgcc gggaagcatc caccgacgct cgtcgtcgac gtgggtggcg
     1321 tgggctacga gctggaagcg ccgatgtcga cgttctacgg cctgccggcg gtcggtgcgg
     1381 aagtgatcct gcacacgcac ctcgtggtgc gcgaggatgc gcacgtgctg ttcgggttcg
     1441 gcaccgagcg tgagcgcagc ctcttccgcg agctcatcaa ggtgtcgggc gtggggcctc
     1501 gcatcgcgct cgcgatcctg tccggcgtca gcgtcgacga attccagcgc tgcgtggagg
     1561 ccgaggacgt cgcgtcgctg gttcgcgtgc c
//
LOCUS       DAOMFD010000338         1583 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_53192_length_1583_cov_39.610602, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000338 DAOMFD010000000
VERSION     DAOMFD010000338.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1583)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1583
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53192_length_1583_cov_39.610602"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="PL152_11645"
     CDS             complement(<1..617)
                     /locus_tag="PL152_11645"
                     /inference="COORDINATES: protein motif:HMM:NF014564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HQR49984.1"
                     /translation="MGPLDGVRILELAGIGPGPFCGMLLADLGAEVVVVDRKGGSLPF
                     NAPPKFDVTRRGKRSIAVDLKQPGAAEVILRLVERCDGLVEGFRPGVMERLGIGPEAC
                     LARNPRLAYGRLTGWGQDGPLAQAAGHDINYVALSGILYHGGHRDSAPSIPPTVVGDI
                     GGGAMFMAVGLLAAILSARSTGQGQVIDAAITDGCALMSALLQGMR"
     gene            complement(626..>1583)
                     /locus_tag="PL152_11650"
     CDS             complement(626..>1583)
                     /locus_tag="PL152_11650"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HQR49985.1"
                     /translation="GGVGGDVRHEAVVAEEMGGHGITSLGHVVHSIVAHYVLNHGTEE
                     QKHRWLPRMATGELVGAIAMTEPGAGSDLQGIKTRAQRVGDEYVVNGSKTFISNGLHA
                     GLVGVVARTDPTKGSKGISIIMVETTDRAGYRVGRVLDKIGQNGWDTAEMFFDDCRVP
                     ADCLLGGVEGQGFIQLMRDLPYERALLALGGVSAMEYALRLTVDYARSRKLFGQSLLE
                     MQNTRFKLAEVKSQVHVARLFIDDCIERLRNDTLDTVTASMAKLWITEAQCRVMDECL
                     QLFGGYGYTTEYPIAKLYVDSRVQRIYGGASEVMKEIVARSL"
BASE COUNT          224 a          583 c          532 g          244 t
ORIGIN      
        1 cgcatcccct gcagcagcgc gctcatgagc gcgcagccgt cggtgatcgc cgcgtcgatc
       61 acctgcccct ggccggtgct gcgggcgctc aggatcgccg cgagcagccc caccgccatg
      121 aacatcgcgc cgccgccgat gtcgccgacc acggtcggcg ggatggacgg cgccgagtcc
      181 cggtggccgc cgtggtagag gatgccgctc agcgcgacgt aattgatgtc gtggcccgcg
      241 gcctgcgcga gcggcccgtc ctgtccccag ccggtgagcc gcccgtaggc caggcgcggg
      301 ttgcgcgcga ggcacgcctc ggggccgatc cccagccgct ccatcacgcc cggccggaat
      361 ccctcgacga ggccgtcgca gcgctcgacg aggcgcagga tcacctccgc cgcgccgggc
      421 tgcttcaggt cgaccgcgat cgagcgcttg ccgcgcctgg tgacgtcgaa cttcggcggc
      481 gcgttgaacg gcagcgagcc gcccttgcgg tccaccacga cgacctcggc gccgaggtcc
      541 gcgagcagca tgccgcagaa cggaccgggg ccgatgccgg cgagctcgag gatgcggacg
      601 ccgtcgagcg gtcccatgcg gcggctcaca gcgacctcgc cacgatctcc ttcatcacct
      661 cgctcgcgcc gccgtagatg cgctgcactc gcgagtcgac gtagagcttg gcgatcgggt
      721 attccgtggt gtagccgtag ccgccgaaca gctgcaggca ctcgtccatc acgcggcact
      781 gggcctcggt gatccagagc ttggccatcg aggcggtgac ggtgtcgagc gtgtcgttgc
      841 gcagccgctc gatgcagtcg tcgatgaaca ggcgtgccac gtgcacctgc gacttcacct
      901 cggccagctt gaagcgcgtg ttctgcatct cgagcagcga ctggccgaac agcttgcgcg
      961 atcgcgcgta gtcgaccgtg agcctgagcg cgtactccat ggcggacacg ccgcccagcg
     1021 cgagcaaggc gcgctcgtac ggcaggtcgc gcatgagctg gatgaagccc tgcccctcga
     1081 ccccgccgag caggcagtcg gcgggaacgc ggcagtcgtc gaagaacatc tccgcggtgt
     1141 cccagccgtt ctgcccgatc ttgtcgagca cgcgcccgac ccggtagccg gcgcgatccg
     1201 tggtctcgac catgatgatc gaaatgccct tggacccctt cgtggggtcg gtgcgcgcga
     1261 cgacgcccac cagcccggcg tgcagcccgt tcgagatgaa ggtcttcgag ccgtttacga
     1321 cgtactcgtc gcccacgcgc tgcgcccgcg tcttgatgcc ctgaaggtcc gagcctgcgc
     1381 ccggctcggt catcgcgatc gcgcccacga gttcgccggt cgccatgcgc ggcagccagc
     1441 ggtgcttctg ctcctcggtg ccgtggttca ggacgtagtg cgccacgatg ctgtgcacca
     1501 cgtgtcccag gctggtgatg ccgtgcccgc ccatctcctc cgccacgacc gcctcgtggc
     1561 gcacgtcgcc accgacgccg ccg
//
LOCUS       DAOMFD010000339         1582 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_53260_length_1582_cov_25.070727, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000339 DAOMFD010000000
VERSION     DAOMFD010000339.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1582)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1582
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53260_length_1582_cov_25.070727"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="PL152_11655"
     CDS             complement(<1..208)
                     /locus_tag="PL152_11655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49986.1"
                     /translation="MQRIPEPELMDEFEQARAYAEADFSEPNQRFVQLFEAEFPALTS
                     GCVVDLGCGPGDIAVRLARRHAGLH"
     gene            complement(241..498)
                     /locus_tag="PL152_11660"
     CDS             complement(241..498)
                     /locus_tag="PL152_11660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49987.1"
                     /translation="MGTRTKITVLGLAFAVLAACGAEQPPQDKAAAQAKGRDTDATVL
                     DDMIQTEDKARGVEATQMAAKAATDAAIDQQTDGGSPDQSR"
     gene            complement(597..1109)
                     /locus_tag="PL152_11665"
     CDS             complement(597..1109)
                     /locus_tag="PL152_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007183373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HQR49988.1"
                     /translation="MGDRYVVFSHGKDSEPWGSKIAAMAEIAREEGFQVESVDYRGID
                     DPKDRVTRLLAFCKDLRGSLVLVGSSMGGYVSVAGSSLLQARGMFLLAPALYMPGYEK
                     FSPRPAACPTTIIHGWRDDVIPVDNSYRFAREQKATLHVVDSDHGLLDQIRAINHYFS
                     YFLFSIEEFL"
     gene            complement(1172..>1582)
                     /locus_tag="PL152_11670"
     CDS             complement(1172..>1582)
                     /locus_tag="PL152_11670"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HQR49989.1"
                     /translation="GPQTVVLVRARRASLASGGMNDAADAILAVVRAVPPGKVTTYGA
                     VAERAGWPGRARLVGHVLGKLPKASGVPWHRVVAAGGRIAFPAGSPSRDRQLRLLRKE
                     GVRAVDGRVDLERDGWGAAPGDLDRLLWRVGDEN"
BASE COUNT          239 a          565 c          519 g          259 t
ORIGIN      
        1 cgtgcagccc ggcgtggcga cgagcgagcc tgaccgcgat gtcgcccggg ccgcaaccca
       61 ggtcgacgac gcagcccgac gtgagcgcgg ggaactcggc ttcgaacagc tgcacgaagc
      121 gttggttcgg ctcggagaag tccgcctcgg cgtaggcgcg agcctgctcg aactcgtcca
      181 tgagttcggg ttccgggatg cgttgcatgg gattccccgt ggcgcgcagg ccgcggccgc
      241 tcacctggac tggtctggcg atccgccgtc cgtctgctgg tcgatcgccg cgtccgtcgc
      301 ggccttcgcg gccatctgcg tcgcctcgac cccgcgcgcc ttgtcctcgg tctggatcat
      361 gtcgtcgagc acggtcgcat cggtgtcgcg gcccttggct tgcgccgcag ccttgtcctg
      421 cggaggctgc tcggcgccgc acgccgcgag gactgcgaac gcgaggccca ggacggtgat
      481 cttcgtgcgc gtgcccatgt gcacctccgg cggacgtggc tgcgcagcat cttacggaaa
      541 ttggggacat tctcctattt ccgcaggggg aaataggaga atgtccccaa tttccgtcag
      601 aggaactctt cgatcgagaa caggaagtag gagaagtagt ggttgatcgc gcggatctgg
      661 tcgagcaggc cgtgatcgga gtcgaccacg tgcagggtcg ccttctgctc gcgcgcaaat
      721 cggtagctgt tgtcgaccgg gatcacgtcg tcgcgccagc cgtggatgat cgtggtcggg
      781 cacgcggccg gccgcgggct gaatttctca tagcccggca tgtacagcgc cggggccagc
      841 aggaacatgc cgcgcgcctg cagcagcgac gaccccgcca cggacacgta gccgcccatg
      901 ctcgagccga ccagcaccag tgagccgcgc aggtccttgc agaacgcgag caggcgcgtc
      961 acgcggtcct tgggatcgtc gatgccgcga tagtccacgg actcgacctg gaagccctcc
     1021 tcgcgcgcga tctcggccat tgccgcgatc ttgctgcccc agggctcgct gtccttgccg
     1081 tgcgagaaga ctacgtaacg atcgcccatt cgacccgtcc tgccactggc ccaccgcgaa
     1141 tcatatgctg cccgtgacgc cgattccgga ctcagttctc atcgccgacg cgccacagca
     1201 aacggtcgag gtcgccgggc gccgcgcccc agccgtcgcg ctcgagatcc acccgtccat
     1261 cgaccgcccg gaccccttcc ttgcgcagca gtcgcagctg tcgatcgcgc gacggcgacc
     1321 ccgccgggaa tgcgatgcga ccgccggccg cgacgacccg gtgccacggc acgccgctcg
     1381 ctttcggtaa tttcccgagc acgtgcccga cgaggcgcgc gcgccctggc cagccggccc
     1441 gctctgccac cgcgccgtag gtcgtcacct ttccgggagg caccgctcgc accacggcga
     1501 ggatcgcatc ggcagcatcg ttcatgccgc cggatgctag cgacgcgcgc cgtgcccgca
     1561 caagcacgac cgtttgcggg cc
//
LOCUS       DAOMFD010000340         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_53740_length_1574_cov_35.163924, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000340 DAOMFD010000000
VERSION     DAOMFD010000340.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53740_length_1574_cov_35.163924"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1355)
                     /locus_tag="PL152_11675"
     CDS             complement(<1..1355)
                     /locus_tag="PL152_11675"
                     /inference="COORDINATES: protein motif:HMM:NF020976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase D family protein"
                     /protein_id="HQR49990.1"
                     /translation="MTNLLFCAISCNGQQDGAWRQFSEFVERSQPSFIIMMGDQVYLD
                     EDKPDIFDDHFHSDSTARRRAMAEKYRLNWSRDDVRRVLANTPTYMVWDDHDIRDGWG
                     SIASDSPTMAARHPRGAEIFRLSTEFFEDARDVYWHFQGCRNPLPGDHREPLTGLPDP
                     AFPNYIDGPLPHGLRRGMPFVFRCGRMMMLVLDSRGERDVFRKDFPILGARQWQFIDD
                     VYARLPPDVDVLAVVTATPIASQDPDGQTQRLMGDRTDDVEAFKRGDEKELFSPKSTK
                     DVTDLLQAVASTKISRFTGQNPNLGNFQISNLDEARDQWSHRFSRREQKDLLTKTFKA
                     RHTNRSAASGREVIFLSGDIHIGCIFDITCLRPPTKAVSLTSSGISQTDDTQPLVGTF
                     IDEEFSLAFGIRSTLRDVVNQFNFGVVQVQPTGNGAEISAVLAHEGNSFVFGLDVKDL
                     I"
BASE COUNT          263 a          488 c          546 g          277 t
ORIGIN      
        1 tagatcaggt ccttaacatc caggccaaac acaaaactgt tgccttcgtg tgcgagcaca
       61 gccgagatct cggcgccgtt gccggttggc tgcacctgga cgacgccgaa gttgaactga
      121 ttcaccacgt cgcgcagcgt cgaacggatg ccgaacgcga gcgagaactc ctcgtcgatg
      181 aaggtgccga ccaggggctg cgtgtcgtcg gtctggctga tgcccgagga cgtcagcgaa
      241 accgccttcg tgggcggccg caggcaggtg atgtcgaaga tgcagccgat gtgtatgtcg
      301 ccggagagga atatcacctc tcgcccggat gctgcgctgc gattcgtgtg gcgcgccttg
      361 aaggtcttgg tcagcaggtc cttctgctcg cgccgggaga agcggtgcga ccactggtcg
      421 cgcgcttcgt cgaggttcga aatctggaag ttgcccaggt tcgggttctg accggtgaac
      481 cggctgatct tggtgctcgc cacggcctgc aggaggtcgg tgacgtcctt ggttgatttg
      541 gggctgaaca attccttctc atcgccgcgc ttgaacgcct cgacgtcgtc ggtgcggtcg
      601 cccatcagcc gttgcgtctg cccgtccggg tcctgcgagg caatcggcgt cgcggtgacg
      661 accgcgagca catcgacgtc cggcggcagg cgcgcgtaca cgtcgtcgat gaactgccac
      721 tgtcgcgcgc cgaggatcgg gaagtcctta cggaagacat cgcgctcgcc gcggctgtcg
      781 agcacgagca tcatcattcg gccgcagcgg aacacgaacg gcatgccgcg acgcagccca
      841 tgcggcagcg gaccatcgat gtagttggga aacgccggat cgggcagccc ggtcagcggt
      901 tcacggtggt cgccgggcag cggattgcgg caaccctgga agtgccagta gacgtcgcgc
      961 gcgtcctcga agaactcggt cgacaggcgg aagatctccg cgccgcgcgg gtgccgcgcc
     1021 gccatcgtcg ggctgtcgga ggcgatcgag ccccagccgt cgcggatgtc gtggtcgtcc
     1081 cagaccatgt aggtcggagt gttggccaga actcgccgca catcgtcgcg cgaccagttg
     1141 agacggtact tttcggccat cgcgcggcgc cgcgccgtgc tgtcggaatg aaagtggtcg
     1201 tcgaagatgt cgggcttgtc ttcgtcgaga tagacctggt cgcccatcat gatgatgaac
     1261 gacggctgcg agcgctcgac gaactcgctg aactgccgcc acgcgccgtc ctgctggccg
     1321 ttgcacgaga tcgcgcagaa cagcaggttg gtcatcgacg ccggcggcgg cagcgtgcgg
     1381 accgttccgc tcgcgccctt gacgaagcgg cccgcgcgca ccacccggta gcggtagcgc
     1441 aggtcgggct tgaggccggt gacggttgcg atcgcggtgc cgaattcgac ggcgccgggc
     1501 tcggtgctga cgaacgggaa gagcccgacg tcttcaacgc gcaggccata gcgcgccggg
     1561 tcgtcggaga cctg
//
LOCUS       DAOMFD010000341         1566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_54243_length_1566_cov_29.591661, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000341 DAOMFD010000000
VERSION     DAOMFD010000341.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54243_length_1566_cov_29.591661"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="PL152_11680"
     CDS             complement(<1..518)
                     /locus_tag="PL152_11680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HQR49991.1"
                     /translation="MTTTRYVTALLATLLAAAPVLAADTTAPTPSAATTPDKPKADDQ
                     PVPQGKVWVTRHKVRIDGQVIAYTATAGTLLMKNDKDEPVALFGFTAYTRDGADPVTR
                     PVMFAYNGGPGSASAWLHMGILGPRRTAIEDLQVNSKGPFRTVDNEYSILDRTDLVMI
                     DPVGTGLSRVVG"
     gene            675..>1566
                     /locus_tag="PL152_11685"
     CDS             675..>1566
                     /locus_tag="PL152_11685"
                     /inference="COORDINATES: protein motif:HMM:NF014998.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004144 - diacylglycerol O-acyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="wax ester/triacylglycerol synthase family
                     O-acyltransferase"
                     /protein_id="HQR49992.1"
                     /translation="MKQLSGSDAMFLQFETGNNFMHVASLAIYDPSTAPGGGVRFKDI
                     LKFFTSRLEQFPQFRRRLVSVPLSLDRPYWIEDPGFDVEFHVRHIALPHPGDWRQLCI
                     QVARIHSRPLDRSKPLWEAYVIEGLHNIPGVPKGSFAVYSKMHHAIVDGESGTELLKA
                     LHSISPEPPDLDEAETRRLLHVDREPTAIELYSRAVVHNLQRVPGLAKFSLGSARRLG
                     TLGAEFAGRIKDEGVFSLKTLRELLSGDLGALLPQMPPKTRFSGDVSAHRVFEAVGLP
                     LSDFKAIRHHVGDVTINDQFL"
BASE COUNT          241 a          534 c          535 g          256 t
ORIGIN      
        1 ttgccgacga ctcgcgacag gccggtgccg accgggtcga tcatgacgag gtcggtgcgg
       61 tcgaggatgc tgtattcgtt gtcgacggtg cggaacggac ccttgctgtt gacctgcagg
      121 tcctcgatgg cggtacgccg cgggccgagg atgcccatgt gcagccaggc ggaggccgag
      181 cccgggccgc cgttgtaggc gaacatgacc ggtcgcgtga ccgggtcggc gccgtccctg
      241 gtgtacgccg tgaagccgaa cagggcgacg ggctcgtcct tgtcgttctt catcagcagc
      301 gttccggcgg tcgccgtgta ggcgatgacc tgcccgtcga tgcggacctt gtgcctggtg
      361 acccatacct tgccttgcgg cacgggctgg tcatcggcct tgggcttgtc cggggtcgtc
      421 gcggcgctgg gcgtcggcgc ggtcgtgtcc gcggcaagaa ccggcgccgc ggccagcagc
      481 gtggccagca gcgcggtcac gtaacgagtc gtcgtcatgg caggcgatcc tccccgggga
      541 aacggcggga gtataggaca gccgtccagc gcagcgcggc aaaggccccg ccgatggact
      601 cggcgacccc cggccagtag tctcacatcc cccttccgaa aacaggcggc cgcgcgcaat
      661 cgcggcagat ccacatgaag cagctctccg gctccgacgc gatgttcctg cagttcgaga
      721 ccgggaacaa tttcatgcac gtggcgtcgc tcgcgatcta cgacccgtcc acggcgccgg
      781 gcggcggcgt gcgcttcaag gacatcctga agttcttcac ctcgcgcctc gagcagttcc
      841 cgcaattccg tcggcggctg gtgagcgtcc cgctgtctct cgaccggccg tactggatcg
      901 aggaccccgg cttcgacgtc gagttccacg tgcggcacat cgcgctgcca cacccaggcg
      961 actggcgaca gctgtgcatc caggtggcgc gcatccattc gcgcccgctc gaccgcagca
     1021 agccgttgtg ggaggcctac gtgatcgagg gcctgcacaa catcccgggc gtgcccaagg
     1081 gcagcttcgc cgtctacagc aagatgcacc acgcgatcgt cgacggcgaa tcgggcaccg
     1141 agctgctgaa ggcgctgcac tcgatctcgc cggagccccc ggacctggac gaggccgaga
     1201 cgcggagact cctgcacgtc gaccgcgagc ccacggcgat cgagctctac tcgcgcgcag
     1261 tggtgcataa cctgcagcgc gtgccgggac tcgcgaagtt ctcgctcggc tccgcgcgtc
     1321 ggctgggcac gctcggcgcg gagttcgccg gccggatcaa ggacgaaggc gtgttcagcc
     1381 tgaagacgct gcgtgaactg ctgtccggcg acctgggcgc gctgctgccg cagatgccgc
     1441 ccaagacccg cttcagcggc gacgtctcgg cgcaccgcgt gttcgaggcg gtcggcctgc
     1501 cgctgtcgga tttcaaggcg atccgccacc acgtgggcga cgtcacgatc aacgaccagt
     1561 tcctgt
//
LOCUS       DAOMFD010000342         1556 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_54845_length_1556_cov_77.676216, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000342 DAOMFD010000000
VERSION     DAOMFD010000342.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54845_length_1556_cov_77.676216"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..932)
                     /gene="rpoA"
                     /locus_tag="PL152_11690"
     CDS             complement(<1..932)
                     /gene="rpoA"
                     /locus_tag="PL152_11690"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007185075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HQR49993.1"
                     /translation="MQSSIAEFLRPRVVKVTPTAPRHARVVIEPFERGFGHTLGNALR
                     RVLLSSMPGSAITEAEIEGVLHEYTSIEGVQEDVVEILLNLKQVAIRLNAREESELRL
                     SKKGPGPVRAGDIQGDHDVEILNPDLVIANLTKAGELVMTLKVEKGRGYRPAGQRLAF
                     EEASRPIGRLLLDASFSPIRKVTYNVEAARVEQRTDLDKLIIDIETNGTIDPEEAIRR
                     AGSILKDQLSVFVDLQGQDEDKIKPSEHHFDPLLLRPVDELELTVRSANCLKAENIHY
                     IGDLVQKTEVELLRTPNLGKKSLTEIKEVLESHGL"
     gene            complement(951..>1556)
                     /gene="rpsD"
                     /locus_tag="PL152_11695"
     CDS             complement(951..>1556)
                     /gene="rpsD"
                     /locus_tag="PL152_11695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HQR49994.1"
                     /translation="PKCKLSRREGTDLFLKSGVKSLESKCKLEVPPGGIKGERRARVS
                     GYGLQLREKQKLRRMYGVLERQFRGYYQKAAGRPGATGETLLQLLEGRLDNVVYRMGF
                     ATTRNEARQLVSHRSVTVNGEVVNVPSFQCKGGEVVALREKAQKQARVQQALQIRGQV
                     GFPDWVEVDEKKFSGVLKSLPERGDILPDINESLVVELYSK"
BASE COUNT          214 a          519 c          539 g          284 t
ORIGIN      
        1 aggccgtggc tctcgagcac ttccttgatc tcggtgagcg acttcttgcc gaggttcggc
       61 gtgcgcagca gctcgacctc ggtcttctgc accaggtcgc cgatgtagtg gatgttctcg
      121 gccttgaggc agttcgcgct gcgcaccgtg agctcgagct cgtcgaccgg acgcagcagc
      181 agcgggtcga agtgatgctc gctcggcttg atcttgtcct cgtcctggcc ctgcaggtcg
      241 acgaacaccg acagctggtc cttgaggatc gagccggcgc ggcggatcgc ctcttcgggg
      301 tcgatcgtgc cgttggtctc gatgtcgatg atcagcttgt cgaggtcggt gcgctgctcg
      361 acgcgcgcgg cctcgacgtt gtaggtgacc ttgcggatcg ggctgaacga cgcgtcgagc
      421 agcaggcggc cgatcgggcg gctcgcctcc tcgaacgcga ggcgctggcc ggccgggcgg
      481 tagccgcggc ccttctcgac cttgagcgtc atcacgagct cgccggcctt ggtcaggttg
      541 gcgatgacga ggtcgggatt gaggatctcg acgtcgtggt cgccctggat gtcgccggcg
      601 cgcaccgggc ccgggccctt cttcgacagg cgcagctcgg actcctcgcg ggcattgagg
      661 cggatcgcga cctgcttgag gttcagcagg atctcgacca cgtcctcctg cacgccctcg
      721 atgctggtgt actcgtggag cacgccctcg atctcggcct cggtgatcgc gctgcccggc
      781 atcgacgaga gcagcacgcg ccggagcgcg ttgcccagcg tgtggccgaa gccgcgctcg
      841 aagggctcga tcaccacgcg ggcgtggcgg ggggccgtcg gcgtcacctt gacgacgcgc
      901 gggcggagga actcggcaat ggacgactgc atgaaagtca ccctgcgtga ttacttggag
      961 tacaactcga cgacgaggct ctcgttgatg tccggcagga tgtcgccgcg ctcgggcagc
     1021 gacttcagca cgccggagaa cttcttctcg tcgacttcga cccagtcggg gaaaccgacc
     1081 tggccacgga tctgcagcgc ctgctgcacg cgggcctgct tctgcgcctt ctcgcgcagc
     1141 gcgacgacct cgccgccctt gcactggaag gacgggacgt tgacgacctc gccgttgacc
     1201 gtcaccgagc ggtggctcac cagctggcgc gcctcgttgc gcgtcgtggc gaagcccatg
     1261 cggtagacga cgttgtcgag gcggccctcg agcagctgca gcagcgtctc gccggtggcg
     1321 cccggacggc cggccgcctt ctggtagtag ccgcggaact ggcgctcgag cacgccgtac
     1381 atgcggcgca gcttctgctt ctcgcgcagc tgcaggccgt agcccgacac gcgcgcgcgg
     1441 cgctcgccct tgatgccgcc cggcggcact tcgagcttgc acttcgactc gagcgacttc
     1501 acgccgctct tcaggaagag gtcggtgccc tcgcggcgcg agagcttgca cttggg
//
LOCUS       DAOMFD010000343         1529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_56633_length_1529_cov_32.426730, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000343 DAOMFD010000000
VERSION     DAOMFD010000343.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1529
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56633_length_1529_cov_32.426730"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1505)
                     /locus_tag="PL152_11700"
     CDS             complement(<1..1505)
                     /locus_tag="PL152_11700"
                     /inference="COORDINATES: protein motif:HMM:TIGR03507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmcA/MtrC family decaheme c-type cytochrome"
                     /protein_id="HQR49995.1"
                     /translation="MNQGTTESAAVGTWVDHGNGTYTYTFKQSVLGIADIPYDPNLTH
                     RVGYEIRINSSGGSSEPYIPANNALYDLVPAGNVPTAITASGRTIVAQTDCDACHQKL
                     EAHGDGRHDVNYCVMCHESYSYDAQTGNTIDFRVMIHKIHMGNDLPSYVASVQAGEPW
                     PYGIYGFYNVFSGYGDYEYLGKKYPGVRYPQDIRNCQTCHHENNADAPQGDAWRTTVN
                     VTTCSACHDTINFTTGEGHGAGGAATDEQCYTCHGPNSAMAGVMTASAHVIPEQAAAA
                     KFKYEVLNVANTAPGQMATVTIRVVDPTNGNAPYDIKAPGGPFQNSAASLTVDVAFST
                     RPDFTNTGSGSATATTGTPAQPIRIDFKANGVPDPAYPGGFTATAATAIPAAATGSGT
                     AFLEGHPGVDLDGNGTPDPVPVQAAEKPFAITDATPVAYSPIVDINRCDNCHKQLALH
                     GNNRVNNVELCAACHNPNATDINRRVAGTKCETVTGTLDDQAIDLKYMIHA"
BASE COUNT          204 a          465 c          553 g          307 t
ORIGIN      
        1 atcgcgtgga tcatgtactt caggtcgatc gcctggtcgt cgagcgtgcc ggtgaccgtc
       61 tcgcacttcg tgccggccac ccggcgattg atgtcggtcg cgttcgggtt gtggcaggcg
      121 gcgcagagct cgacgttgtt gacgcggttg ttgccgtgga gcgccagctg cttgtggcag
      181 ttgtcgcacc ggttgatgtc cacgatcggc gaataggcca ccggagtggc gtcggtgatc
      241 gcaaagggct tctcggcggc ctgcaccggc acgggatcgg gcgtgccgtt gccgtccagg
      301 tcgacgcccg ggtgcccttc caggaacgca gtgccagagc cggtggcagc agccgggatc
      361 gcagtcgccg cggtcgccgt gaatccgccg gggtaggcgg gatcgggcac gccgttcgcc
      421 ttgaagtcga tgcggatcgg ctgggcgggc gtacccgtgg tcgcggtagc ggaaccgctg
      481 cccgtgttgg tgaagtccgg cctcgtcgag aacgcgacgt ccaccgtgag cgaggccgcg
      541 gaattctgga acgggccacc aggcgccttg atgtcgtacg gcgcattgcc gttggtcgga
      601 tccacgacgc ggatcgtcac ggtggccatc tggcccggcg cggtgttcgc gacgttcagg
      661 acctcgtact tgaacttcgc cgcggccgcc tgctcgggaa tcacgtgcgc gctggccgtc
      721 attacgccgg ccatggcgga gttcggaccg tggcaggtgt agcactgctc gtcggtcgcc
      781 gcgccgcccg cgccgtgtcc ctcgccagtc gtgaagttga tcgtgtcgtg gcaggcgctg
      841 caggtggtga cgttgaccgt cgtgcgccac gcgtcgccct gcggcgcatc cgcgttgttc
      901 tcgtggtggc aggtctggca gttgcggatg tcctgcgggt acctgacgcc cgggtacttc
      961 ttgcccaggt actcgtagtc gccatagcca ctgaagacgt tgtagaagcc atagatccca
     1021 tagggccagg gctcgcccgc ctgcacgctc gcgacgtagc tcggcaggtc gttgcccatg
     1081 tggatcttgt ggatcatcac gcggaagtcg atcgtgttgc cggtctgcgc gtcgtacgag
     1141 tacgactcgt ggcacatgac gcagtagttc acgtcgtggc ggccgtcccc atgcgcctcg
     1201 agcttctggt gacatgcgtc gcagtcggtc tgcgcgacga tcgtgcgtcc ggacgcggtg
     1261 atggccgtcg gcacgttgcc cgccggaacc aggtcgtaaa gcgcattgtt cgcggggatg
     1321 tagggttcgc tcgagccacc cgacgagttg atgcggatct cgtagccgac gcggtgcgtg
     1381 aggttcgggt cgtacgggat gtcagcgatg cccagcacgc tctgcttgaa cgtgtaggtg
     1441 taggtgccgt tgccgtggtc cacccacgtg ccgacggcgg ccgactcggt ggtgccctgg
     1501 ttcacgggtg gcgaattcgc cgtgcaggt
//
LOCUS       DAOMFD010000344         1521 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_57156_length_1521_cov_47.558663, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000344 DAOMFD010000000
VERSION     DAOMFD010000344.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1521)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1521
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57156_length_1521_cov_47.558663"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="PL152_11705"
     CDS             complement(<1..866)
                     /locus_tag="PL152_11705"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HQR49996.1"
                     /translation="MTPASRFVPYSYARDNAILLVPKSERDAEVWISDATPLAALNEV
                     MRVFPGKVKPIVVEPTKLTEAISQTYGDQSGSAQQIVGDIEGEFDITRMMQEVPDVED
                     LLETEDDAPIIRMINALLTQAARDGASDIHIEPFESYSLVRFRVDGTLRDVVRPKRAL
                     HAALVSRIKIMAALDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEK
                     DLSKLELTNLGMSKEALDRFDTLIHQPHGIILVTGPTGSGKSTTLYAAIRRLDKNTTN
                     IMTVEDPIEYDI"
     gene            complement(889..1179)
                     /locus_tag="PL152_11710"
     CDS             complement(889..1179)
                     /locus_tag="PL152_11710"
                     /inference="COORDINATES: protein motif:HMM:NF016895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HQR49997.1"
                     /translation="MAAPSSTGAFAATQGASTGPASNLLQVAAVSEISRGRELQRQLR
                     AAGFDAYWESVKSKKGADIVRVRVSVDPATQSMAETVARLKAMGFDPIVVTP"
BASE COUNT          211 a          499 c          533 g          278 t
ORIGIN      
        1 tcgatgtcgt actcgatcgg gtcctcgacc gtcatgatgt tggtcgtgtt cttgtcgagc
       61 cggcgtattg cggcgtagag cgtcgtcgac ttgcccgagc cggtcgggcc ggtcaccagg
      121 atgatcccgt gcggctggtg aatcagcgta tcgaaccggt cgagcgcttc cttcgacatg
      181 ccgaggttgg tcagttcgag tttggagaga tccttctcga gcagacgcag caccgcgcgc
      241 tcgccatggc cggtcggcaa ggtcgaaaca cgcacgtcca cgggacggcc gccgacccgc
      301 aatgtgatcc gtccgtcctg cggcaggcgc ttctccgcga tgtcgagcgc ggccatgatc
      361 ttgatacgcg acaccagcgc ggcatgcagc gcacgcttcg gccgcacgac gtcgcgcagc
      421 gtgccgtcga cgcggaagcg caccagcgag taggactcga atggctcgat gtggatgtcg
      481 ctcgcgccgt cgcgcgccgc ctgcgtcagc agcgcgttga tcatgcggat gatcggcgcg
      541 tcgtcctcgg tctcgagcag gtcctcgacg tccgggacct cctgcatcat gcgcgtgatg
      601 tcgaactcgc cctcgatgtc gccgacgatc tgctgcgccg acccgctctg gtcgccatag
      661 gtctgcgaga tcgcttcggt gagcttggtc ggctcgacga cgatcggctt gacctttccg
      721 ggaaacaccc gcatcacttc attgagtgcg gcgagcggcg tggcgtccga gatccagacc
      781 tcggcgtcgc gctcgctctt cggcaccagc aggatcgcgt tgtcccgcgc gtacgaatag
      841 ggcacgaagc ggctggcggg agtcacggta tccgtccggc ggtgctgatc agggcgtgac
      901 aacgatcggg tcgaagccca tcgccttcag gcgcgcgacc gtctcggcca tgctctgcgt
      961 agccgggtcg accgaaacgc gcacgcgcac gatgtctgcg cccttcttgg acttcacgct
     1021 ttcccagtag gcgtcgaacc ccgcggctcg cagctgcctt tgcagctcac ggccgcggct
     1081 gatttccgag acggcggcga cctgcagcag attgcttgcc ggccccgttg atgcgccctg
     1141 tgttgccgcg aaagcgcccg tggagctggg cgccgccact gacgacacgg ctggatttgc
     1201 aacaaccggg gcgggctcaa cggttgcagt cgtggcgacc ggtgggatcg atgctgccgc
     1261 ggctggcgcg gcctgggtgg gtgcaccgac gacgggttcc ggcggcgcct ctgcagcccg
     1321 ctcgacgggc gtcgttggcg ctgcggcact ggccggcggg ctcacgttcg cagcggtcgc
     1381 caggtccttc ggcggtgccg aacgcggctc ggggctcgta ggggctaatg cggtcgtgtc
     1441 tggccgtgga gcgggcatgt cgcgcgacgg cgctggcgcg ggcgccgcgg tactcggtgc
     1501 ggcagcgtca gtcgtcgcaa c
//
LOCUS       DAOMFD010000345         1509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Steroidobacteraceae bacterium isolate AMR_MDS_5011
            NODE_57970_length_1509_cov_41.884457, whole genome shotgun
            sequence.
ACCESSION   DAOMFD010000345 DAOMFD010000000
VERSION     DAOMFD010000345.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32867170
            Sequence Read Archive: SRR5278469
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Steroidobacteraceae bacterium (wastewater metagenome)
  ORGANISM  Steroidobacteraceae bacterium
            Bacteria; Pseudomonadota; Gammaproteobacteria; Steroidobacterales;
            Steroidobacteraceae.
REFERENCE   1  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/01/2023 17:09:41
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,345
            CDSs (total)                      :: 2,328
            Genes (coding)                    :: 2,321
            CDSs (with protein)               :: 2,321
            Genes (RNA)                       :: 17
            tRNAs                             :: 14
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 0 of 7
            Pseudo Genes (incomplete)         :: 7 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1509
                     /organism="Steroidobacteraceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57970_length_1509_cov_41.884457"
                     /isolate="AMR_MDS_5011"
                     /isolation_source="Ecological wastewater treatment"
                     /db_xref="taxon:2689616"
                     /environmental_sample
                     /geo_loc_name="China"
                     /collection_date="2015-07-01"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..344)
                     /locus_tag="PL152_11715"
     CDS             complement(174..344)
                     /locus_tag="PL152_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003087298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit 3"
                     /protein_id="HQR49998.1"
                     /translation="MDAGTLRGIFTLVVMLAFVGLVAWAYSRRRKADFDEMANLPFHE
                     YPPGKEPGSKTP"
     gene            complement(348..956)
                     /gene="ccoO"
                     /locus_tag="PL152_11720"
     CDS             complement(348..956)
                     /gene="ccoO"
                     /locus_tag="PL152_11720"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007184050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome-c oxidase, cbb3-type subunit II"
                     /protein_id="HQR49999.1"
                     /translation="MNHESVEKNVGLMGVLIALVISFGGLVEIVPLYFQGAGTQPAPG
                     VVPYDALRLAGRDVYIREGCYLCHSQMIRPFRSETERYGPYSVAGESVYDHPFQFGSK
                     RTGPDLARVGGRYSDDWHRVHLMNPRDVVPESNMPRFPWLKDAKLDGPLVSRKMEALR
                     KVGVPYTDADIAGAPAAVEGKTELDALVAYLQGLGTNARAGN"
     gene            complement(970..>1509)
                     /locus_tag="PL152_11725"
     CDS             complement(970..>1509)
                     /locus_tag="PL152_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009425113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HQR50000.1"
                     /translation="GAWHKLRTDPILKFMIVSLSFYGMSTFEGPMMSIKTVNALSHYT
                     DWTIGHVHSGALGWVAMISIGTMYALIPKLYGREQMYSTRMIDVHFWTTTIGVVLYIT
                     SMWIAGVMQGLMWRATNPDGTLTYAFIESIKSTYPYYAVRLLGGTLVLAGMFMMAWNV
                     WKTVAARTSTADAPVVQPA"
BASE COUNT          243 a          501 c          504 g          261 t
ORIGIN      
        1 cggcagcggg ttgttgtact cgcgcaggtc cttgtcccac acgtgcccgg tgtcggcccc
       61 gccgccgatc ttctctccgg gcgccctggg tttggacgtc cagcgcagca gccagacaca
      121 ggcgaggatg ttgaggatcg tcacgaggac gacgaagaga ctccaaccgc tagtcatggg
      181 gtcttgctcc ctggttcttt tccgggcgga tactcgtgga acggcaggtt cgccatctca
      241 tcgaaatccg ccttgcggcg tctgctgtag gcccatgcca cgagtccgac gaacgcgagc
      301 atcacgacga gcgtgaagat cccgcgcagc gtgccggcgt ccatggctca gttgcccgcc
      361 cttgcgttcg tgcccaggcc ctgcaggtag gcgacgagcg cgtcgagctc ggtcttgccc
      421 tcgaccgctg ccggcgcgcc ggcgatgtcg gcgtcggtgt acgggacccc gaccttgcgc
      481 agcgcctcca tcttcctgga caccagcggg ccgtcgagct tggcgtcctt gagccacggg
      541 aagcgcggca tgttggactc cggcacgacg tcgcgcgggt tcatcaggtg cacgcggtgc
      601 cagtcgtccg agtagcggcc gccgacgcgc gcgaggtcgg gcccggtgcg cttggagccg
      661 aactggaacg ggtggtcgta caccgactcg ccggcgaccg agtacgggcc gtagcgctcg
      721 gtctcggagc ggaacggccg gatcatctgc gagtggcaca ggtagcagcc ctcgcggatg
      781 tacacgtcgc gcccggcgag gcgcagcgcg tcgtacggca cgacacccgg cgccggttgc
      841 gtccccgccc cctggaagta cagcggcacg atctcgacca ggccgccgaa gctgatgacg
      901 agcgcgatca gcacgcccat caggccgaca tttttttcga ctgattcgtg gttcattcgg
      961 ctctccccat caggccggct gcacgaccgg ggcgtccgcc gtgctggtgc gcgccgcgac
     1021 cgtcttccag acgttccagg ccatcatgaa catgcccgcg aggacgagcg tgccgccgag
     1081 caggcgcacc gcgtagtacg ggtaggtcga cttgatcgac tcgatgaacg cgtaggtcag
     1141 ggtcccgtcc gggttcgtcg cgcgccacat caggccctgc atcacgccgg cgatccacat
     1201 cgaggtgatg tagagcacca cgccgatggt ggtggtccag aagtgcacgt cgatcatgcg
     1261 cgtgctgtac atctgctcgc ggccgtacag cttcgggatc agcgcgtaca tcgtgccgat
     1321 cgagatcatc gccacccagc cgagcgcgcc ggagtgcacg tggccgatcg tccagtcggt
     1381 gtagtgcgag agggcgttga ccgtcttgat cgacatcatc ggcccttcga aggtcgacat
     1441 gccgtagaac gacagcgaca cgatcatgaa cttgaggatc gggtcggtgc gcagcttatg
     1501 ccacgcgcc
//
